LOCUS       DAKTLH010000001        61839 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.001, whole genome shotgun sequence.
ACCESSION   DAKTLH010000001 DAKTLH010000000
VERSION     DAKTLH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 61839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61839
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.001"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            164..367
                     /locus_tag="O0394_000001"
     CDS             164..367
                     /locus_tag="O0394_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498118.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            550..1485
                     /locus_tag="O0394_000002"
     CDS             550..1485
                     /locus_tag="O0394_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCY0498119.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPETLDE"
     gene            complement(1567..2535)
                     /locus_tag="O0394_000003"
     CDS             complement(1567..2535)
                     /locus_tag="O0394_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0498120.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVKVDKHFGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(2668..3012)
                     /locus_tag="O0394_000004"
     CDS             complement(2668..3012)
                     /locus_tag="O0394_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0498121.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEH"
     gene            complement(3054..3464)
                     /locus_tag="O0394_000005"
     CDS             complement(3054..3464)
                     /locus_tag="O0394_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCY0498122.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(3844..5910)
                     /locus_tag="O0394_000006"
     CDS             complement(3844..5910)
                     /locus_tag="O0394_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCY0498123.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGIGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            complement(6211..7950)
                     /locus_tag="O0394_000007"
     CDS             complement(6211..7950)
                     /locus_tag="O0394_000007"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCY0498124.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAIASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(7997..8686)
                     /locus_tag="O0394_000008"
     CDS             complement(7997..8686)
                     /locus_tag="O0394_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCY0498125.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(8679..9074)
                     /gene="cidA"
                     /locus_tag="O0394_000009"
     CDS             complement(8679..9074)
                     /gene="cidA"
                     /locus_tag="O0394_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HCY0498126.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(9287..10165)
                     /locus_tag="O0394_000010"
     CDS             complement(9287..10165)
                     /locus_tag="O0394_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0498127.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            10332..10550
                     /locus_tag="O0394_000011"
     CDS             10332..10550
                     /locus_tag="O0394_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCY0498128.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            10939..11370
                     /locus_tag="O0394_000012"
     CDS             10939..11370
                     /locus_tag="O0394_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0498129.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
     gene            complement(11635..11739)
                     /locus_tag="O0394_000013"
     CDS             complement(11635..11739)
                     /locus_tag="O0394_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498130.1"
                     /translation="MLNIKAIKPSEKGIKVLSHQFKQVSKTQLNYFFN"
     gene            complement(11902..12036)
                     /locus_tag="O0394_000014"
     CDS             complement(11902..12036)
                     /locus_tag="O0394_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498131.1"
                     /translation="MTDKNGHGIAITGIILFVINALQINNDLPISISSKLKKYIHKPD
                     "
     gene            complement(12130..12387)
                     /locus_tag="O0394_000015"
     CDS             complement(12130..12387)
                     /locus_tag="O0394_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498132.1"
                     /translation="MEIIIEIYMYLRDGIKEHFILKKVRKKFKKLNEINSDIQNMYAE
                     NQGLLETDKTIIDKVLSTDVTNSHDINELYLFLKNYLDKTQ"
     gene            complement(12966..14243)
                     /locus_tag="O0394_000016"
     CDS             complement(12966..14243)
                     /locus_tag="O0394_000016"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCY0498133.1"
                     /translation="MQNLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAKVAEALKKEPRANTQAAEHILQEIRQQ"
     gene            14497..15663
                     /locus_tag="O0394_000017"
     CDS             14497..15663
                     /locus_tag="O0394_000017"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCY0498134.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(15833..16354)
                     /locus_tag="O0394_000018"
     CDS             complement(15833..16354)
                     /locus_tag="O0394_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCY0498135.1"
                     /translation="MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLA
                     IFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVG
                     KLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
     gene            16763..18868
                     /gene="clpL"
                     /locus_tag="O0394_000019"
     CDS             16763..18868
                     /gene="clpL"
                     /locus_tag="O0394_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HCY0498136.1"
                     /translation="MNNGFFNSDFDSIFRRMMKDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGGNHSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNILKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            complement(18937..19107)
                     /locus_tag="O0394_000020"
     CDS             complement(18937..19107)
                     /locus_tag="O0394_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCY0498137.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDINRDCCG
                     TEQHTANHFPGK"
     gene            complement(19120..21114)
                     /gene="feoB"
                     /locus_tag="O0394_000021"
     CDS             complement(19120..21114)
                     /gene="feoB"
                     /locus_tag="O0394_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCY0498138.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKYLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAEQVGAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(21126..21353)
                     /locus_tag="O0394_000022"
     CDS             complement(21126..21353)
                     /locus_tag="O0394_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCY0498139.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(21569..24058)
                     /gene="farE"
                     /locus_tag="O0394_000023"
     CDS             complement(21569..24058)
                     /gene="farE"
                     /locus_tag="O0394_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HCY0498140.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAG
                     SAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVVFAVLSALTLLPALISIFHKRI
                     KVNKLQSKFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFASVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKHQDAHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQL
                     NSKKQATNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            24203..24751
                     /gene="farR"
                     /locus_tag="O0394_000024"
     CDS             24203..24751
                     /gene="farR"
                     /locus_tag="O0394_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HCY0498141.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTMSDTINNKEDLREIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFIHWIKDE
                     KIDWPGEDIDNIFHRLINIKIK"
     gene            complement(24828..24995)
                     /locus_tag="O0394_000025"
     CDS             complement(24828..24995)
                     /locus_tag="O0394_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498142.1"
                     /translation="MRYVISIIMGIVLAIWSFKQLSQSHLDSGFIFFFIVYVLCISCF
                     NSDKHDKNKKR"
     gene            complement(25107..26651)
                     /gene="pruA"
                     /locus_tag="O0394_000026"
     CDS             complement(25107..26651)
                     /gene="pruA"
                     /locus_tag="O0394_000026"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HCY0498143.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(26841..27440)
                     /locus_tag="O0394_000027"
     CDS             complement(26841..27440)
                     /locus_tag="O0394_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCY0498144.1"
                     /translation="MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATN
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            27679..27870
                     /gene="cwrA"
                     /locus_tag="O0394_000028"
     CDS             27679..27870
                     /gene="cwrA"
                     /locus_tag="O0394_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HCY0498145.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            28110..30518
                     /locus_tag="O0394_000029"
     CDS             28110..30518
                     /locus_tag="O0394_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCY0498146.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLILTETTTFKAVPGHGIEATIDH
                     HYILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
     gene            30809..31015
                     /gene="copZ"
                     /locus_tag="O0394_000030"
     CDS             30809..31015
                     /gene="copZ"
                     /locus_tag="O0394_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCY0498147.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
     gene            complement(31105..32103)
                     /locus_tag="O0394_000031"
     CDS             complement(31105..32103)
                     /locus_tag="O0394_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCY0498148.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNFIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(32126..33280)
                     /locus_tag="O0394_000032"
     CDS             complement(32126..33280)
                     /locus_tag="O0394_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0498149.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSKVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            complement(33707..35215)
                     /locus_tag="O0394_000033"
     CDS             complement(33707..35215)
                     /locus_tag="O0394_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0498150.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYEDYIELRQLRYIYDVYFDHDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIERGV"
     gene            complement(35227..36090)
                     /locus_tag="O0394_000034"
     CDS             complement(35227..36090)
                     /locus_tag="O0394_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HCY0498151.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFDNERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            complement(36127..37254)
                     /gene="crtQ"
                     /locus_tag="O0394_000035"
     CDS             complement(36127..37254)
                     /gene="crtQ"
                     /locus_tag="O0394_000035"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HCY0498152.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            complement(37260..38753)
                     /locus_tag="O0394_000036"
     CDS             complement(37260..38753)
                     /locus_tag="O0394_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0498153.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            complement(38746..39234)
                     /gene="crtO"
                     /locus_tag="O0394_000037"
     CDS             complement(38746..39234)
                     /gene="crtO"
                     /locus_tag="O0394_000037"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HCY0498154.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGPRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            complement(39409..40176)
                     /locus_tag="O0394_000038"
     CDS             complement(39409..40176)
                     /locus_tag="O0394_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0498155.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            complement(40537..42348)
                     /locus_tag="O0394_000039"
     CDS             complement(40537..42348)
                     /locus_tag="O0394_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0498156.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
     gene            complement(42859..43347)
                     /locus_tag="O0394_000040"
     CDS             complement(42859..43347)
                     /locus_tag="O0394_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0498157.1"
                     /translation="MAINIIEYNRSYKEELIEFILSIQKNEFNIKIDRDDQPDLENIE
                     HNYLNNGGQFWLAINNHQNIVGTIGLIRLDNNMSALKKMFVDKGYRNLKIGKKLLDKV
                     IMTCKEQNIDGIYLGTIDKFISAQYFYSNNGFREIKRGDLPSSFPKLDVDNRFYYRNL
                     KD"
     gene            complement(43623..44324)
                     /gene="isaA"
                     /locus_tag="O0394_000041"
     CDS             complement(43623..44324)
                     /gene="isaA"
                     /locus_tag="O0394_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HCY0498158.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSISYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
     gene            complement(44932..45987)
                     /locus_tag="O0394_000042"
     CDS             complement(44932..45987)
                     /locus_tag="O0394_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCY0498159.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            complement(46114..46242)
                     /locus_tag="O0394_000043"
     CDS             complement(46114..46242)
                     /locus_tag="O0394_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498160.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSENSEFCDILFFIVLSYR"
     gene            46397..46966
                     /locus_tag="O0394_000044"
     CDS             46397..46966
                     /locus_tag="O0394_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0498161.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            47164..47664
                     /locus_tag="O0394_000045"
     CDS             47164..47664
                     /locus_tag="O0394_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498162.1"
                     /translation="MTSIFVFAYLNNTSGNMLFRQQMICACLLANVRHSSIVCLNNIC
                     CIVDMRCLNTKIGGIDVSETDEPQGFERTHNILNINQSSLGVVTYITNKLKSTLKQHI
                     IIARGKKRIDYRLSYNFYTTYYDNVEIKKIDCEYTYAMPIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            47884..48114
                     /locus_tag="O0394_000046"
     CDS             47884..48114
                     /locus_tag="O0394_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCY0498163.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(48264..48641)
                     /locus_tag="O0394_000047"
     CDS             complement(48264..48641)
                     /locus_tag="O0394_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0498164.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(48658..49479)
                     /locus_tag="O0394_000048"
     CDS             complement(48658..49479)
                     /locus_tag="O0394_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HCY0498165.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK"
     gene            complement(49499..49798)
                     /locus_tag="O0394_000049"
     CDS             complement(49499..49798)
                     /locus_tag="O0394_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HCY0498166.1"
                     /translation="MSLNKEQRRITAEELQAHFEASTLSVQMIAEKLNVTTEDVEKVL
                     AMTAPLGIFSHQLQRFIHLVWDVRDVINDNIKGNGQTPEPYTYLKGEKEDYWFLR"
     gene            49929..50486
                     /locus_tag="O0394_000050"
     CDS             49929..50486
                     /locus_tag="O0394_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0498167.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVGRND"
     gene            50479..51183
                     /locus_tag="O0394_000051"
     CDS             50479..51183
                     /locus_tag="O0394_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCY0498168.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGA
                     DGPTGTFINGDGEMPW"
     gene            51281..52291
                     /locus_tag="O0394_000052"
     CDS             51281..52291
                     /locus_tag="O0394_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0498169.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPVSYYFKNNFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSP
                     NN"
     gene            52311..53141
                     /locus_tag="O0394_000053"
     CDS             52311..53141
                     /locus_tag="O0394_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0498170.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWLEFEIRQYTHSNITLDDFTKY
                     SDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(53288..54178)
                     /locus_tag="O0394_000054"
     CDS             complement(53288..54178)
                     /locus_tag="O0394_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCY0498171.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHKSLHQMINHQFVQ
                     SPVKCTKAEFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(54276..55616)
                     /locus_tag="O0394_000055"
     CDS             complement(54276..55616)
                     /locus_tag="O0394_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HCY0498172.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            complement(55610..56716)
                     /locus_tag="O0394_000056"
     CDS             complement(55610..56716)
                     /locus_tag="O0394_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HCY0498173.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTEIYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGKYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVLTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQDVIENYQKNQMYLDDYSCCEVSCTC"
     gene            complement(57050..57346)
                     /locus_tag="O0394_000057"
     CDS             complement(57050..57346)
                     /locus_tag="O0394_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HCY0498174.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFENMKQNLDSEIQRGEVTQQ"
     gene            complement(57334..57702)
                     /locus_tag="O0394_000058"
     CDS             complement(57334..57702)
                     /locus_tag="O0394_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498175.1"
                     /translation="MSNKLDEINKIITAKHEQMDDLYDEKREVKALIDESDALNHSID
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKVEEEMIDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            complement(57717..57995)
                     /locus_tag="O0394_000059"
     CDS             complement(57717..57995)
                     /locus_tag="O0394_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HCY0498176.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLMSKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
     gene            complement(58337..59203)
                     /locus_tag="O0394_000060"
     CDS             complement(58337..59203)
                     /locus_tag="O0394_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCY0498177.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRLDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLQDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMDKILQDTTS"
     gene            59421..60485
                     /locus_tag="O0394_000061"
     CDS             59421..60485
                     /locus_tag="O0394_000061"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCY0498178.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTAFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            60640..60915
                     /locus_tag="O0394_000062"
     CDS             60640..60915
                     /locus_tag="O0394_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498179.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMM"
     gene            complement(61101..61823)
                     /locus_tag="O0394_000063"
     CDS             complement(61101..61823)
                     /locus_tag="O0394_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCY0498180.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
BASE COUNT        19971 a        11530 c         9403 g        20935 t
ORIGIN      
        1 agataattaa agtaggatat tatttttagt tttctgatag gaatgatgat agtctatagg
       61 ttgaatcttt actttttttt aaagctaaat ttacatcaac ttaacaatgg ttggttatac
      121 tgaagatgaa aatatttagt ataacttagt ggaggcgata aaggtgcaat tgagtcattc
      181 cgttaaagtt gcaatttcta tctatttagc acttatcttt ataacgttca cttcttattt
      241 agtcattatt ttatatacga gtatgactgg acatgatgta tcacatttcg tgttagatag
      301 tcagcattct catcatggat ctcttacgca aaaacatttg agtcttcctg aaatctcatt
      361 taaatagttt atcctctgtg tttcaacatt catttcccat atcgattcat ttatctatca
      421 tctagaccac tacatcttag atgatttttt tattttctca tttcactctt tcttaaaagt
      481 cgatataatg aattaaatca ttatcataca ccgacatatt ttatgttgtt ggtgttaagt
      541 tttaaagggg tgagatactt ggcgaataat cattcagctt tgcgtttaaa aggaattata
      601 cttgccattg tcggtgcttg tttatgggga ttaggtggta ctgtttctga tttcttgttc
      661 aaatataaga atattaatgt cgattggtac gtcactgctc gacttgtagt cagtggtgtt
      721 ttcttactta ttatgtacaa aatgatgcaa cccaaacgtt caatatttag cgtattccaa
      781 gatcgacgta tgttaggcaa attacttatc ttcagtatac tgggcatgtt agtagtacaa
      841 tatgcttata tggcatctat taatacaggt aatgctgcga ttgcaacatt actacaatac
      901 attgcgccag tttatattat tatttggttt gtcataagag gcgttgcaaa actaacatta
      961 tttgatgtgc ttgctattat catgacacta ttaggaacat ttttattatt aacaaatggt
     1021 tcattttcta atttagtcgt caatcctgca agtttattct ggggtatttt agctggtgta
     1081 gcactcgctt tttacacaat ttatccttca gacctactta accgcttcgg ttcgattcta
     1141 attgtcgggt gggcaatgct tatttctggt gttgcgatga atttacgcca tccaatttgg
     1201 cacattgata tcactaaatg ggacatatca attatattat ttttaatctt tggtattatc
     1261 ggtggtaccg cactcgcatt ttatttcttt atcgacagtt tacaatacat atcagcgaaa
     1321 gaaacaacat tattcggaac tgttgaacct gtcgtagccg ttatcgcaag cagtctatgg
     1381 ttacatgtgg cattcaaacc atttcaaatc gtaggcatca ttcttattat gattttaatt
     1441 ttattactat cacttaaaag acaacctgaa acattagatg aataagaaaa ctctgataat
     1501 cactttagca agtaactatt atttaacaac gtagttacct tataggtgat atcagagttt
     1561 tttattttag ttaataatat ttttcacttg gtataaaaag cgtcgtcgct ctggtatcgg
     1621 aaatactgga taaatatgga attggtaata tccaggtata aagtcatgtt cgataccttt
     1681 cgcactcaac aattgcgata agttcaaagc atctgggtac agtacttctt ttgtaccaac
     1741 agttaatgta atacgaccta aaccatctaa gtcaccatta attggtgata ctttgtagtt
     1801 atctaaaggt gtgtcccctg cccattgttc agctaagaac acactgccat ccacacctac
     1861 cattgggtct ttctttaagt agtcaggaat ttcaggatgc tgcatcgttg catctaaaac
     1921 tggtgaaatt aatacaatat gtcctggttg cacaatatgt ttttctttta acaattgagc
     1981 aaatgataaa gcaatttgac cgcccgcaga gtcacccatt acaacgattt gtttagaatc
     2041 tgctacttga ttcaataaat catggtacaa cttttcaaaa agcacatacg tcgcttgata
     2101 atcttgatgc ggaatcttcg gatatactgg catgatgact ttagcattga gtgtttctgc
     2161 aagttcatca ataaattcga aatgaatttt gagtgggtct tggaaccatg cgcctccatg
     2221 tgcatataat acaacgcgtt gatgcttatc ttgtttatcg ttaacggtat aaacggttga
     2281 accgaagtgc ttatccactt ttactggcat cttaaactga taattggttc cctcatattc
     2341 gcctgctgtc tgtggcgcca ttttttctaa tgctttcatc gcttcttctt tatacataaa
     2401 acgacgctta gctctagata atcgtatacc ttgttctaac atataacttt tcacactgcg
     2461 tttttcttta attctaatat gtgcgtatgc tgcaactaaa aatccaaacg caagtgtaga
     2521 ccatttttta cgcatttgtc gtcactcctc tttgatgatt tctactatag ttctactgta
     2581 ccatgctgat tctaattact accaatattt actgggaaat aagttatgat gtctacataa
     2641 tcttagcaat gctaatcatt tgcgatatta atgctcatcg acatcgttat ttacactatc
     2701 attcatttgc ttaagatgat gttcaaaacg ttgcatatcg ataaaacgcc cttgacgatg
     2761 catttctttt ccattcataa aaattaaatc cacaggtaca gtgaaaatat ttaattctcc
     2821 agcaatagct tccacctgac tttgattaat cacagctaat ggcacattgg gatatgattg
     2881 catcaagtct tcaatttgtg gtaaaacggc atgacacacg ctacactgat cgcgcatgac
     2941 atgtacaact gctaacggat attgatgaat aaattcacgg aatgcttcga cactatttaa
     3001 ttgcttaacc atttcgaaca ccttcttact ttttatattt taaaatgaag tcattatttt
     3061 gttgctaaaa tgtcttgaaa cacttgtggc acagaggtta tcttcttatc actcaatctg
     3121 taaaaaccgc ctgtctgtgt accaatacaa tgcacgccat gtgcggacac aatttcaaac
     3181 tgaaacaccc atttcatctt gcttgcttta actaagtaaa tacgacctac tggtttgtca
     3241 tgtattgtga caggtacttt atactctgct tccgttttca taagtatagg tgaaatgcct
     3301 tgattgatca ttgttgagta attcatgtgc tgattaataa acgctgttct caaatcttcg
     3361 aaccatctaa tataaacgat attactaacg acacccatcg catcaatgtc gtaaccattc
     3421 acttcgattt ctttctctgc aactaataga ccttctgttg tcataatgac aaatctcctt
     3481 atcttacaaa tagatttaaa atcatgttaa tttattttta ttactttcat gataaaaata
     3541 caagcaattg aactttatta cggaaaatca aacttttcaa tgagactatt aatttggcta
     3601 gtcgcgaaga tgcatttttc taaaatgtga caaaaacata agcaatgttt tctatatcta
     3661 acactaatat ttaacttatt atcatcaaca cacctgctat acatcattgc gcactaaaaa
     3721 ggcagtagct tcattttaag ctactgcctt ttgtaacgta ttctctttca actcgctact
     3781 tcgcatacgc actacgaatg actaaaagtt cattcatttg atatgctgtt tcatttttaa
     3841 tcattatttg actgtcataa ttagcttacc tggatcagca tcttgtacat cttcaatgac
     3901 aagttcttta ttttcaagat ttgtaataat cattggtgta acgatgcttg gtgcgtgtgc
     3961 tttcaagtat gctaaattca ctttcattaa tggttgacct tgtgttactt tttcatcaac
     4021 gttaatcaga ctttcgaatc cttcaccatt cagtttcact gtatcgatac caatatgaat
     4081 aagtacttcg acgccacttt cagattctaa tcctatcgca tgtttcgtag ggaagattgt
     4141 tttcactgta ccatcaaacg gtgctacaat ttcacctttt tcagggataa agccaacacc
     4201 atcacccatc attttaccag cgaacacttg atcaggtact tctgataatg gaatgatttg
     4261 accgatacct ggtgcataga tgtctgttgt tccaagtgct tctacgtgaa ctggttcatc
     4321 tgacatttct tcagtcactg tcgtttcact tggtttcgta atttcaccac tcataatctt
     4381 ggccatatca tgtttaattt gatctgattt tggaccaaag atagcttgca tattgtttcc
     4441 aacttctaat acacctgatg cgcctaaagc tttaatacct gctacatcta cttttgattt
     4501 atcaaccact tctacgcgta gacgtgtaat acatgcatct aaatgtttaa tgttttcttt
     4561 tccacccatt gcatctaaga catcaaatgg taattttgcg acactagagt tacgaatttc
     4621 agtttcttca tcttcacgac ctggtgtttt caatttaaac ttacgaattg caaagtcgaa
     4681 taagaagtaa tacacgatag catatacaat accgactgga ataactaata atgcgtgtga
     4741 acgatcccag tttaataaac catataaaat ataatctatg aaaccacctg agaatgtcat
     4801 accaatttta acgcctaata aatgcattac taagaatgat gtaccagcta ataatacgtg
     4861 aattccataa agtactggtg ctacaaataa gaatgaaaat tctaatggct cagtgatacc
     4921 agttaaaaat gcagttaatc ctgctgataa cattaaacca cccacgactt ttttacgttc
     4981 tggtcgtgca tttttataaa tagcaaatgc cgccgctggt aaaccaaaca tcataaatgg
     5041 atatttacca gtagtaaatg caccagcagt aaatggtacg ccatctttca attgtgccat
     5101 ccaaatacgt tggtcaccac gaaccaattc acctgcgtga tttgtataac ttccgaattc
     5161 aaaccagaac ggtgaataga aaatatgatg taaaccaaat ggaattaatg agcgttcaat
     5221 aataccgaat ataaacgttg ttaatgttaa atttttattt aataagaaat tcgataaact
     5281 atttaatcca tcttgaattg gtggccacgc aaagcttaaa agcacacctg ttgcgattgc
     5341 tacgaccgat gtcacaatcg gtacaaatcg tttacctgca aagaatccta aaaatggtgg
     5401 tagtgtaata ttataaaatt tgttgtaaca ccatgcggct aaagcaccca tgataatacc
     5461 accaaacaca cccgtttgta acgttggaat acctaatact aaagcatgtg ctggttcttt
     5521 cgctgcttga cttaattctt ttgccccttt ggcatatgag aaaatgtcat caattgtaat
     5581 gtgcaacact ttccccattg ttgcattcat aattaagtaa cctactagcg ctgctaatgc
     5641 tgcaacaccg tctcctcctg ctaatccaag tgctgtacca actgcaaata ataatggcaa
     5701 gttatcaaat acaacttgtc ctgctgcttc catgaccgac gaaatcatta caatgatatc
     5761 gttttttaac catggtgcaa tttctactaa ttgttcgttg tgcattgcgt taccaaacgc
     5821 taataaaata ccagctgctg gtaaaatcgc aacaggtaac attaatgctt taccgatacg
     5881 ttgcaattgt ccaaacaatt tcttaaacat ataaacatcc cctatctcaa tttccgcttc
     5941 catgtacaaa aaaagcaggc atgaacaaac aagactattc tcatagtgcg gttgtttgct
     6001 catgcctgca ttaccagtaa cacgcaagtg atatctaatt catagctatt atacatattt
     6061 gtaaaagcgt tttcaacaac tttttcacaa ttttatcatc aattcagtac gatcatttgt
     6121 gataaataaa caaaacggca attcccattt tgttgaaata gaaattaccg tttttatagt
     6181 tcaattcaat tgtgataacc tttaaatcag ttataagaaa cgttttgctg ccactgaaat
     6241 tggtggaatt tgatctaaat ctaaatgttt atcttcagta attgatctaa atgcccactt
     6301 accataacca agcgcttctt catttacaat tttacctggt aatggcgcag cattaggatc
     6361 aacatataca tctacaatcg ttggtacatc ttgtgctaat gcctcttcga ctatagcatc
     6421 tacttcgcta gcactcttaa ttgtataacc tttaccacct gctgcctcag caaattttgc
     6481 atgatccata tcagaaaaat caactgcata ttctaattca ccagctgctt gttgttcata
     6541 tttaataaat gctaactgtt tgttattaag tacaaataca gttaaaggta aatcatattg
     6601 tactgctgta gcgaagtctt gcattaccat ttggaatgca ccgtcaccag caattgcgat
     6661 ggcttgtcta tttggatatg caatttttga tgcaattgca cctggaagac cgcaacccat
     6721 tgtacctaac caacttgaaa tgatgaactt gttatttaca ccaaggttta agtatcgagt
     6781 tgaccaaact gttgctgtac ctacatctgc tgaaatcact gcatcatctt taataaattt
     6841 attgattgat gccattaatc gttctggacg taatggttta ctattattat ttttatcttg
     6901 ttccatccat ttatcccaaa ccgctttacg ttctaacgtt ttgtttaaga atggtctttc
     6961 agcaacatgt ttaatatttt cagttaactg atgcaacgca attttactat ctccaacaat
     7021 tcctacatta atattgaaac gatgtccgat atttttagga tttgtgtcaa tttgaatggc
     7081 tttaatattt ttcttaggta agtaatccac atatggatag tttgtaccaa ccataattaa
     7141 taaatccgct tcctgcattg tttgataaga tgttttggta ccgattttac ctaagttacc
     7201 gatactatat ggatgatcat ccggcaagat tgttttagct ggtaatgaat gaatgacagg
     7261 aattttagcc atttcaataa attcacgtag ctcatctttc gcatgtttcg cacctacacc
     7321 aattaacatg acaggctttt tacttttatt aattagttta accgcttttt tgatgtcttt
     7381 atattttggt gatactactg ttggtcttga tgtatctact ggtttattcg ttgtatcttt
     7441 aattttttca gttaataagt cgttaggaca aataacaaca gctacacctt tttgttcata
     7501 tgccgtacga attgcttcgt taacgatttc aaacacattg tcaccttttt caatttggtg
     7561 attataaacg gctacatctt cacataattt ttgtaaattt gtttcttgga atgctttcgt
     7621 tccaagtgct gtactattcg tttgtccaga taatattaat tgcggtacat tatccatttt
     7681 ggcatcatac ataccattta ataaatgaat taaaccaggg ccaccgatac ttaatgccac
     7741 accgatttta ccagttaatt ttgtgtaacc agcagccgct aagcttgcta cttcttcatg
     7801 acgtacatga taaaatttaa attgatctct cactgtacgt aaactatcga ctactgcgtc
     7861 gattgagtct cctggaatac catacaagtg atctatatcc catgcttgta atgctttaac
     7921 taatgcttca tttgctttta tttttgccat tattactaat agcctccctt tctgtctatc
     7981 attttaaata tctaaattaa tagaataata aaattagaac aggtattaac actgaactaa
     8041 taactgcagt taaaatcatc ccaattgaac taaatgcacc ggattcaata tccatttcta
     8101 gtgctttagc tgtgccaaat gcatgtgacg cattcccata cgttaatcct ttggcgatag
     8161 aagattcaaa tcttccaaat cttaataaca tcgaacctaa aatactaccg attaaacccg
     8221 ttgtgataat aaaaagtacg gtcatcgtat ctgtaccacc tagttcatgt gacacttcga
     8281 tacctactgc ggctgttata gatcggggca ataacgttac aatgacgtct ttagaatagc
     8341 caaatgcttt aagtgttaag aacactaaca tgaaattcag catgacgcca gttaatacac
     8401 ttgcaaaaat gatagagaca ttgtctttaa ttttctctct atttttataa agtgggtacg
     8461 ctaaacatac gaccgttgcg tttaaaatat ggttgatcca actgccacct ttcatatacc
     8521 cgttataact aattccaaag ataagtaaga caaaaataat tcctaaagat gcaattaatg
     8581 ctggattcaa aaatgggttc ggatattttt gttgtaacct tttagcgaaa taatataaaa
     8641 cgacagtcaa caaaatcatt aataaggctt gcacgtaatc attcataagc gtctacacct
     8701 ttacgatgtt tatgtttaac agacattttt tcagcaatat aacctgaaga taatgcaacg
     8761 atacatgttc ctatgataat gactgcgaaa aagagtatat aatttagcgt aatttcggaa
     8821 gcaacatcca taatacctac aactgacggt atgaagaaaa agaccatcgt ctttaataaa
     8881 aagtttgcac cgtcttctac ccaagttagc ggtacaatct taaattgtaa tagtaaataa
     8941 aataaaaata gaccaacaat actgccggct aagggaagat gaaaaatctt ttgaatttct
     9001 gtgccaatat aagtaatcac aatgatgatt cctagttgta gtagtagttt gattattaat
     9061 tggactttgt gcatggcgcc atccctttct aaatatgtct aaattgttac aataactatt
     9121 ataaagatgg cgtacacaat ataaaaatac tcttttttta tagtttgtat tactattttc
     9181 tatggaaacg ctctctaaat ttcacttcat tttcacaaaa aaacactcat tatttcaatg
     9241 acccacgtaa tgatgcgcct taaataatga gtgctttatt aatatgttag cctaaacggt
     9301 ctttcaaaaa ttctatccat ttcgttgtgg catgacttaa ttgtttatcc ttcttccaaa
     9361 caacacctaa ttcccaatgt acatgagcat cttcaatgcg tagcagcttc acatctccat
     9421 ttagttgctc tgaaattgat gttggtaaaa tactaatacc taattcattc gtaactaaat
     9481 cttctataac atgccattgt gaaatttgcg ctacagtatt cggaacaaag ccaacgtttt
     9541 tagcattttc aataatttta tcattcaagt aaaagtcttt attaaataaa atgaagtctt
     9601 cacctgctaa atcttcgagt ttaacagttt catattttgc gagtcgatgc tctctgctca
     9661 cgataagtcg caaatcttcc ttatctaggg tagtataatc gaaaatatga tgatcgactg
     9721 gcaaagtggt cacgcctata tctacttcat cattgataat ttgctgttca attgttttac
     9781 cgccattttc tatcaaatta tatgtaacat ttggatattg ttgatggaat gcaccaagaa
     9841 tattgataag aatcttcata ttcatgactg ccgacatgcc catgtttata tgtcctgttt
     9901 ccagtccatt caagcgttcc atttcagatg gtaaataatc atacagtgca acaatttctt
     9961 tacttttctc ataaaaaatt tgacctgcat cggtaagaat taaatgtctt ttacttctat
    10021 caaataaggg cgtgcccatt tcattttcaa tatctttaat tgctttacta attgtaggtt
    10081 gtgcaatata taatgatttg gaagcattag tcatgcctcc ttgcttaacg acttcaataa
    10141 aatatttcat atgtttgata tccactaaca tcatcctaac tatttcaaca cgtttatttc
    10201 attatacaca acttcttaaa cattcaataa atttagattt ataaaattgc tttataaaaa
    10261 gttttatgta aaatacacat tcttttatgg tattatatta atggctcaag tatattaatc
    10321 gaggtgaggt attgagcttt tacgaattta tgcaaagttt tcttggtgat gacacaccat
    10381 taggtgaatt ggttgattgg attaatcaag atagcaattt ccctagagaa gtgaagagcc
    10441 atagtgaaat attgtcatat tttcgaacaa atccatgtcc tgaaagcatc tcagtacctg
    10501 taattaaacg ggcactatca gttttcaacc aattcacaaa tgtacaatga tacatgaata
    10561 catggagtca catcatctca tgttagcgct ctgattgcag ggcgctattt ttaatgttat
    10621 tgctacattt agtatattgt gattgctatt ttctttcagt tatagaacca tgtacctttt
    10681 ttttaaatca ttttatattt tcccactaat atccgctgtt aatcaatcct gacatccttg
    10741 tatcactatg acaattaatt gttaaataca tgaatttcta cattttatga aaaaatccat
    10801 ttttattaca attcaacact ttatatgaca acttcattac agttactttt attgttgatt
    10861 gcttacattg ttttctaaaa aaaatttgtt atcataatta acgttgaata aagaaaaaaa
    10921 ttaacttggg agataaaaat ggaatataaa aagatactaa ttcgtttatt aattgctttt
    10981 gcagtacttt tctcagcaga tttcacttat caatccgtcg aacaaacgca tcaatcgcat
    11041 gccgcagtta attattatag taaaaaccaa tgtacatggt gggcatttaa acgtcgcgca
    11101 caagtcggta aacctgtttc taatagatgg ggcaatgcta aaaattggta ttacaatgca
    11161 cgtaaatcaa aatatgcgac tggtcgtaca ccaagaaaat ttgctgtcat gcaatcaact
    11221 gcaggatatt atggacatgt cgcagttgtt gaacaagtat ataaaaacgg tagtattaaa
    11281 gtttcagaat acaactttta tcgcccatta aaatacaata cacgtgtact aagcaaaaag
    11341 gcagcacgta actttaacta tatttactaa tcaaaaaact tctatcacga acgcttcaat
    11401 ttcctgtatg cgtgtgatag aagtttttat tttatgaaat tatattatta cttctacaaa
    11461 tttcaaattg ccgtaattga acgtatattt cttcttcaac tattatttca tctttagcat
    11521 aatctatata taaaatttta tgctattatt taaataattc gctataactt aacatacgtt
    11581 ttcgatataa accttgttct aaatctcaat aattttttgc tgttttcatc gtcattagtt
    11641 aaaaaaataa tttaactgag tttttgagac ctgtttaaat tgatgactca aaacctttat
    11701 ccccttttca ctcggtttaa ttgctttaat atttaacaca gtctcattgt aattttgcta
    11761 ctaagtttga aatatttcga ttcgaatgtg aaacatcatt tattattact ttcgacagct
    11821 agaaaaatat tgttaaacca aaactataat taccactttt atatagaact atatataaat
    11881 tttaactttg agacagaact gctagtcagg tttatgaata tatttcttta gtttacttga
    11941 tatacttatt ggtaaatcat tatttatttg aagagcattt ataacaaaaa gtataatccc
    12001 agttatagcg attccatgac catttttatc agtcaaagat tcatcaacaa aaactttttg
    12061 ataaatttat atatttgtat aatttattat ggtagatatc tacacccgta tctataacag
    12121 ctacagttat cattgcgtct tatccaaata gtttttaaga aataaatata attcattaat
    12181 atcatggcta tttgtaacgt cagtgcttaa gaccttgtca attattgttt tatctgtttc
    12241 taagagcccc tgattttctg catacatatt ttgtatatcg ctgttaatct catttaattt
    12301 tttaaatttt tttctaacct ttttcaaaat aaaatgctct ttaattccat ctcttaaata
    12361 catataaatc tctattataa tctccataaa tgtcatatct cctctctcaa aaaacattat
    12421 actcaatata ttaaaaataa gaaaggtttt ttcagaattt ttagtatttt cagatattta
    12481 ttcttaagta tcttattatt acttcttttt aacgctagtg gaataattaa taataattgt
    12541 atttaaatgc aatgctgttg ttctttcata cttacaagca agtcataaga aatgagaaat
    12601 taaattctta tatgatgaat aaatgatgac tgcagaagaa attgctaaaa aaggagtgga
    12661 gtgaggcatt ctactgttca tattgttaac aaatatcaaa atgataggaa cttgaagaat
    12721 ctaagactgt taaaatcgag acaataagaa actcgttcca aatttgaatc ctataatgaa
    12781 ttcgtctcat gaagaagtta tttacttttc attagactga tttactaatc aaaaaacttc
    12841 tatcacgaac gcttcaattt cctgtatgcg tgtgatagaa gtttttattt ttgttcatat
    12901 taatttatct aagcgctacg atggactgac ttaaactttt ctttaaaatc gctattcgcc
    12961 atttactatt gttgtctaat ttcttgtaaa atatgttccg ctgcttgtgt atttgcacgg
    13021 ggttcttttt tcaaagcttc agctacttta gcaatttcat caccttttgc ccctacaacg
    13081 atagctaatg atttatattg taaactcata tgaccttgtt gaataccttc tgacacaagc
    13141 gcgcgacatg ctgcaaagtt ttgcgctaaa ccaacggcag caactacatg acctaattct
    13201 tgtgctgact ctacatttag tagctctaat gaagctttag caattggtaa tacttttgta
    13261 ccaccgccaa cgattgccaa tgtcataggc acttcaattg taccaatcaa tcgttgacga
    13321 tcttgatcgt aacgccatgt agcaatacca cgatactgtc cgtcacgact cgcgtatgca
    13381 tgcgcacttg cttctgcacc acgcgtatca tttcctgttg ctaaaacaac agcatgtatg
    13441 ccattcataa cacctttatt atgtgttgct gcacgatgaa tatctacttg ggccaataca
    13501 gaagcacgtt ccattcgttt ggcaacctct tctccagttc tctcgcccct tgctaaatct
    13561 ttaacatcaa tttcgccttg aactttaaca acggacgctg ttgcatgatt ggataaaata
    13621 ctcattaaaa tgtcgctttg cggaaattca ttttttaaaa atgcagttat ggcctctaaa
    13681 atcgtattaa gcatattagc gcccatagca tctttcgtat caacaaatac ttttaaagat
    13741 agtaactgtt gctcaggaaa tgtatcaatc gctatacgtt ggtaaccacc accacgcgct
    13801 ttaatagaag gatatgcctc atccgcaatt ttatgaattt gcttttctaa agctttaatg
    13861 tctgctgata atttttcagt atcgtcaacg ccatcaaaga cgatttgacc tatcataata
    13921 cgttcagaag ataccgtttt aaatccgcca gtctgattca ctagctttgc accataacta
    13981 gctgcagcga caactgaagg ctcttccacc atcataggta caacatatgc cttatcgtcc
    14041 acaatgatat tcggtaataa tccaacgggt aatgcacctt gcgcgatgac attttcaatt
    14101 aaactattgg ctacttcttc atcgattaat ggatgattca gtaaaatgtc gaattgttct
    14161 tctgataacc attgcttatc aaccaattgt tgtaactttt ctttacgaga taaatgtcga
    14221 aaattcttat ctaaattttg catggacgta ctccttttac ttcacataat ttttaacatt
    14281 tcaatcacta ctatttttac cacaaaataa cgtcattcgt cttaaaattc aattgaataa
    14341 ttgtcgtttt gactttaaaa taaaacaagg taaattaaaa cgcttacaag aaacgacaaa
    14401 tcatttttaa atttagtata tttctttgta taaaattagc atattctgat atgatacaag
    14461 tgttgctttt ataaatttga aaggatgtaa aaccttatga caataggtat cgataaaata
    14521 aacttttacg ttccaaagta ctatgtagac atggctaaat tagcagaagc acgccaagta
    14581 gacccaaaca aatttttaat tggaattggt caaactgaaa tggctgttag tcctgtaaac
    14641 caagacatcg tttcaatggg cgctaacgct gctaaggaca ttataacaga cgaagacaaa
    14701 aagaaaattg gtatggtaat tgtggcaact gaatcagcag ttgatgctgc taaagcagcc
    14761 gctgttcaaa ttcacaactt attaggtatt caaccttttg cacgctgctt tgaaatgaaa
    14821 gaagcttgtt atgctgcaac accagcaatt caattagcta aagattattt agcaactaga
    14881 ccgaatgaaa aagtattagt tattgctaca gatacagcac gttatggatt gaattcaggc
    14941 ggcgagccaa cacaaggtgc tggcgcagtt gcgatggtta ttgcacataa tccaagcatt
    15001 ttggcattaa atgaagatgc tgttgcttac actgaagacg tttatgattt ctggcgtcca
    15061 actggacata aatatccatt agttgatggt gcattatcta aagatgctta tatccgctca
    15121 ttccaacaaa gctggaatga atacgcaaaa cgtcaaggta agtcgctagc tgacttcgca
    15181 tctctatgct tccatgttcc atttacaaaa atgggtaaaa aggcattaga gtcaatcatt
    15241 gataacgctg atgaaacaac tcaagagcgt ttacgttcag gatatgaaga tgctgtagat
    15301 tataaccgtt atgtcggtaa tatttatact ggatcattat atttaagcct aatatcatta
    15361 cttgaaaatc gagatttaca agctggtgaa acaatcggtt tattcagtta tggctcaggt
    15421 tcagttggtg aattttatag tgcgacatta gttgaaggct acaaagatca tttagatcaa
    15481 gctgcacata aagcattatt aaataaccgt actgaagtat ctgttgatgc atatgaaaca
    15541 ttcttcaaac gttttgatga cgttgaattt gacgaagaac aagatgctgt tcatgaagat
    15601 cgtcatattt tctacttatc aaatattgaa aataacgttc gtgaatatca cagaccagag
    15661 tagtcggtgt atttaaaaca catataataa aacctaaaag cagcagtaag accacttcta
    15721 attgaaatcg tcttactgct gttctctatt tataacactt cgtattgaat gaattcatta
    15781 tgcctatttg acacattatt gaagttttcc taatgcctgg atcctttata cgttacggct
    15841 tcgtgctatg ttttggtaca taaagctttg acatatcgat attctccaac tctaacagct
    15901 taattttatt attaatcgtt ccaccgaacc ctgttaagct acccgtttta ccgacaacac
    15961 gatgacatgg cacgataata gataatggat tacttccgac tgcacctcca accgcttggg
    16021 ctgacatttt tggcttgtta agcagcttgc ctactttttt ggcaatagca ccatacgttg
    16081 ttagagtccc ataaggaacc tgtcttaatt cattccaaac acactgttga aaatgactac
    16141 ctgttggctt taaaggtatt gtgatttcag gattgtcacc tttaaaatac gcgtctaacc
    16201 actgtgtcgc ctctctaaat atcgctaaag acgtattttc ttccctagta ccatcacctt
    16261 gttgattttc aaacaaaaca gcggtcagac ttaccccatc actcaaaagt tccaatcgtc
    16321 ctacaggcga atcatagtaa ctcttatact ccataaaaat cccccctttt tctaatcata
    16381 cgccttaatt gttaaattag aaaatattca tgttcacatt ttttaaaaat aaataagtcg
    16441 tcaattacgt atataaacac gtaataccag ctatcacttt gctgcaatat acagttacat
    16501 atcttactac acgtgctaac ctcttacttt gtaaaccaaa tcttaaatta aaatattgaa
    16561 aatgcaatga atccttaata ttttattaaa cctataatta cttattaaaa ataacacaca
    16621 atattcataa agttttaaaa atattctgtt ttatcaccta ctattagtgg aaaagtacaa
    16681 ttgcaattgt atatagtttg cataacgctt caaaagtaat ttcttttttg tttagttcaa
    16741 aaaaatttag aggtgatgtt atatgaataa cggttttttc aatagcgact ttgattcaat
    16801 ttttcgaaga atgatgaaag atatgcaagg ttcaaatcaa gtcggaaaca aaaagtacta
    16861 tattaatggt aaagaagttt cacctgaaga actagcgcaa ctcacacaac aaggtggcaa
    16921 tcactctgct gaacaaagtg cgcaagcttt tcaacaagca gcacaaagac aacaagggca
    16981 acaaggtggc aacggcaatt atttagaaca aattggtcgt aaccttacgc aagaagcacg
    17041 tgacggttta ttagatccag tcattggtcg tgataaagaa attcaagaaa ctgctgaagt
    17101 tttaagtaga cgaactaaaa acaatcctat attagttgga gaagctggtg ttggtaaaac
    17161 tgcgattgtt gaaggtttag cacaggcaat cgttgaagga aatgtaccag cagcaatcaa
    17221 agacaaagaa attatttctg tagacatttc atcattagaa gctggaacgc aatatcgtgg
    17281 tgcttttgaa gaaaatattc aaaaattaat cgaaggtgtt aaatcttcac aaaatgccgt
    17341 actattcttt gatgaaatcc atcaaattat cggttcaggt gccacaggaa gtgattcagg
    17401 tagcaaaggg ttatctgata ttttgaaacc tgcattaagt cgtggtgaga tttctattat
    17461 tggtgcaaca acacaagatg aatatcgaaa caatattctt aaagatgctg cattaacgcg
    17521 cagatttaat gaagtgcttg ttaatgaacc aagcgctaaa gatactgttg aaattttaaa
    17581 aggtattcgc gaaaaattcg aagaacacca tcaagtaaaa ttaccagatg acgtattaaa
    17641 agcatgtgtt gacttatcaa ttcaatatat tccacaacga ttattaccag ataaagcaat
    17701 cgatgtgtta gatattacag cagcacattt atctgcgcaa agtccagctg tcgataaagt
    17761 tgaaactgaa aaacgaattt ctgaattaga aaatgataaa cgtaaagcag taagtgctga
    17821 agaatataaa aaagctgacg acattcaaaa tgaaatcaaa tcattacaag ataaattaga
    17881 aaatagtaat ggtgaacata ctgctgttgc tacagttcat gatatttcag atactattca
    17941 acgattaact ggtattccag tttctcaaat ggatgataac gatattgaac gtttaaaaaa
    18001 tatttctaat cgtttaagaa gtaaaatcat aggtcaagat caagctgtag aaatggtttc
    18061 acgtgcaatt cgccgtaatc gtgctgggtt tgatgacggc aaccgtccaa ttggcagttt
    18121 cctatttgtt ggccctactg gtgttggtaa aacagagctt gctaaacaat tagcaattga
    18181 tttatttggt aataaagatg cactgattcg acttgatatg agtgaatata gtgacacaac
    18241 agctgtttca aaaatgattg gtacaactgc tggttatgtt ggttatgatg acaattcaaa
    18301 tacgttaact gaaaaagtac gccgtaatcc atactcagtc attctatttg atgaaatcga
    18361 aaaagcaaat ccacaaattt taacattgtt attacaagta atggatgatg gtaatttgac
    18421 tgatggtcaa ggtaatgtca tcaactttaa aaatacaatt attatttgta catcaaatgc
    18481 tggctttggc aatggcaatg acgctgaaga aaaagatatt atgcacgaaa tgaaaaaatt
    18541 cttccgccct gaattcctta accgcttcaa cggcatcgtt gaattcttac atttagataa
    18601 agatgcattg caagatatcg tcaacttatt attagacgat gtacaagtta cattagacaa
    18661 aaaaggtatt acgatggacg tttctcaaga tgcgaaagat tggttaattg aagaaggcta
    18721 tgatgaagaa ttaggtgcac gtccattaag acgtattgtt gaacagcaag tacgtgacaa
    18781 aattacagat tactatttag atcatacaga cgttaaacat gtggatatag atgttgagga
    18841 taacgaatta gtcgtaaaag gtaaataacg acactttaac atatcgcgca tcaaaaatga
    18901 gcatcaggtc gcccttgcct gtgctcattt ttttaattat ttccctggaa aatgattcgc
    18961 tgtgtgctgt tctgttccac aacaatcacg attaatgtca catgtaccac attttccttg
    19021 ttttgaacgc ttgaaaaatt ttactagtgt atataaggca tatccgaaaa ttgctaaaaa
    19081 aattaaaatg ttaataatga ctgacacttt aaccactcct taaacaaata aatgtccgac
    19141 ttgataaaaa atgaatgtta agacatatgc agtgactaga ggataggcaa ctgcaagtgc
    19201 cgtccatttc catgaataag tctctttacg gattgctgct actgtagaaa cacaaggaat
    19261 atacaatagt ataaatatca taaatgcata cgcagatagc ggtgtgaatt gattttgaat
    19321 cacattaaca aggcctgcat cacctgatga atagataatc gccatcgaac ttacgataac
    19381 ttcttttgct aaaaatcctg gcactaacgt agcacctgct tgccatgttc caaatccgag
    19441 cggttgcact aacataccaa agaaactacc aaccatatgt aaaaaacttt gattgatatt
    19501 cacattgata ccatgtggtc ctacataact taatagccaa atgactactg agccgccaaa
    19561 aataaatgta cctgctttac gaacaaagcc cttagccttt tcccaagtac tacgccacaa
    19621 cgttttaatg gaaggcacac ggtatgttgg caattccaca ataaagattg cattatcatt
    19681 ttttaaaatc gtcttagtaa gtactgtact gactaaaaat gccataataa tacctaaaac
    19741 atacaggctt aatactacta aagattgatt ctctttgaaa aagataccta cgaacaacgc
    19801 atacactggc agtctagcag agcatgacat gaatggtgca attaatatcg ttgttaaacg
    19861 ctctttttca ttttcaatac tgcgcgcagc cataatactc ggtacattac aaccaaatcc
    19921 gataatcatt ggtataaaag acttcccgct taaaccgaac gattccataa tacgatccat
    19981 taaaaccgca atacgtgcca tataacccga atcttctaat aaagatataa agaaaaagag
    20041 cacaacaatt tgtggtacaa agactaatac tgatcctaca ccagcaataa tgccatctgt
    20101 aattaaatct tgtaaaaatg gtataacacc aagataattc ataatcgtct tcacactatc
    20161 tgtaaatgta ccacctataa atgcatcgag ttgatccgac aaaggtgtgc caatccatgt
    20221 aaatgtagtt tgaaagatca accacataat tgctagaaag ataggcatcc ctatatattt
    20281 atgtgttaat atcttgtcta ttctagagct gaaatattgc ttatcttcat ctggatacgt
    20341 taccacgtct tgcaataacg tctcaatata atgattgcgt atacgctcca tctctcgacg
    20401 aacagataca gcccctactt gttcagcaac ttgatcacgt aaactcgaca atttatttac
    20461 aacctctgaa ttaagttcgt ttgcaatttg catgttattt aataagaatt gaatcgcaat
    20521 aaacctagct tgatacttat catgagatgt ctctgtcatt attatttgac acatattttt
    20581 aattgtctct tcaatcttct caccataatt gattttaaaa tgcggttgat acccttcccc
    20641 tagatgcttt atttcgccaa gtaaatattt tgttcctttg cctgtacgtg ccacaactgg
    20701 aaaaataggt gtttttaact ttttcatcaa tttatgataa tcgattttta tcccgcgctt
    20761 tgtagctaca tcaatcatat ttaatccgat gtatattggt tgattaagtt ctaacaattg
    20821 tactgttaat tgcatatttc tttttagttg actcgcatca acaatgttaa tgattcctga
    20881 aaatgaatcg tttaataaat agtctgtcac tacagtttca tctttagaaa tcggcgataa
    20941 atcatatgta cctggtaaat caattaattg tcctacattt tctttaagtt tccctacttt
    21001 tttctctacc gttacgccac tccagttgcc tatatattca tacgaaccag ttaaagcgtt
    21061 aaacaaagat gttttaccaa cattaggatt tcctaaaata caataatttt ccattcgtcc
    21121 ggctcctatt cttctaatgc aatagaacaa gcatcgcaat gtctaatact taactgttgt
    21181 ccgtttactt caataataca tggcccttta aataaacatt tttgtttaat cgttatgata
    21241 gcgtcatctg ttaacccaaa ggcacttaga cgatacaaca tattctcatt agcaatatcc
    21301 attcgcttta ttttataagc cttattcatt tcaccatttt taatgtttaa catactattt
    21361 cgctctccta ttagaaataa taatcattat cacttaaaaa tcataaccct taaaattgta
    21421 gctcgcaata ctttatttaa ataattttca tttttcatgt aaaatttgtg acattgcaaa
    21481 aatgttttgt ataatcactt ccatgcaaaa aacctccgct tcaaatgtca ttgatagaca
    21541 tttaaagcgg aggcatgtac atgttatttt atgatttatt tcgtagattt ttttctaaca
    21601 attgattgag cttttcaata tttttattca actgttctat attcgttgct tgtttttttg
    21661 aatttagttg atgcttgttt tgataatcta ttaaagcatc atataagaac aatgaacttg
    21721 ctgattgctg tttcacttct tggtacaatg ccatcgtttt atcatctagt actattgaat
    21781 gtgcatcatc ttgcccatgt gcatcttgat gtttcgatac atttgttgca ccttgtggat
    21841 atactttaaa ggttgtgtcg tatgtttcct tactatcttt agcttcagat tcctgtgatt
    21901 caaccgtttt atatttttca agtgcatgtc cttcaatatc aactcgtgga ataatgcgat
    21961 tcaaccatgc tggtaaatac cacgaacctt ttccaaacaa tttcgttaat gcaggaatta
    22021 acatcattct gactacgaag gcatcaaaga gtacaccaaa cgctaatgcc atacccattg
    22081 atttaatcat gacatcttct tggaatacaa acgcaaagaa gacactaaac ataattaatg
    22141 cagctgctac aataacagga ccgctttctt tcaatcctac tttgatagaa taatcattat
    22201 cccctgtttt actatattct tcatgaattc gcgacataag gaagacttca taatccatcg
    22261 ctaatccaaa taagatacct atagtaataa ccggtaaaaa tgctagcatt ggtcctgtcg
    22321 tttcaatacc aaacagacct ttcataaaac catcttgcat tactaatgtt gtaaatccta
    22381 atgttgccat taatgacaag acgaatccta aaactgcttt taatggtatt agaattgaac
    22441 ggaagacaat cattaataag aaaaatgcta atacaacaat gactgaggca aataaaggta
    22501 tcgcctcatt taacttttta gacatatcaa tattaatgac actttgtccc gaaatctccg
    22561 ttttgaaccc atatttatct tgtgcatctt tatgataatc tcgtaaatca tgcactaaat
    22621 catttgtact ctctgcatta ggcccttgct taggtatcac gaccatcaaa gcgtaatcat
    22681 tatctttact catttgtggt ggcgtaacga tatctacatt tttcttatct ttaatatctt
    22741 tatatacaga ctgtaaatct tgttgtaatc cttgtggatc atccttttta tctttcacat
    22801 ttatcaacat cggtatttgg ccattaaatc cttcaccaaa tttatccgag ataatatcgt
    22861 aagctttttt ctgtgtagaa tctgctggtt taacaccgtc atctggaata ccaagtcgca
    22921 tatgactaac tggtattgca gctgctacta atatgattaa acctagtaat actgccgcaa
    22981 gtgcatttcc tgtaataaat ttagaccatg gcgtatcaat atcttttttg aatttagact
    23041 gtaatttatt cactttaatg cgtttatgga aaatgcttat taatgcaggt aataaagtta
    23101 aagcgctaag tactgcaaaa acaacactaa ttgccgaagc aaatcccatt accgctaaga
    23161 agtcaatgcc tactaatgat aaaccacata ctgcaattac aactgttaca ccagcaaaaa
    23221 caactgcact acctgctgtt cctattgcaa gaccaatgcc tttaatgtaa tctgtttcag
    23281 ttttcataac ttgtcgatat ctgaataaaa taaataatgc ataatcgata ccaactgcta
    23341 gtccaatcat tacggctaat gtcagtgtga catttggtat atcgaatgca taagttaaca
    23401 aactgataat acctacacca gaggctagac caatcaatgc acttataatt ggtaatcctg
    23461 cagcaatgac tgaaccgaat gtgattaaca gtacaacaaa tgcaacaata ataccaacta
    23521 gttcagaatt accgcctact tctgtagatg tcattcctgt accagttaac tctgtttgta
    23581 cattatgatt gtcacgtaag tctttcaaat gacttttaac attatctcta gagccatctt
    23641 ttaaagatgt ttgactaacg tcatatgtga tatctgcaaa tgcagttgtt ttatctttac
    23701 taatttgctt attttcataa ggatctgata ttttatcaat gtgcttgtca tcttttttaa
    23761 tatcatctaa cgttttctta atatctttag taatgttcgg ttgcacaata ccatcatctt
    23821 tagtcgtctt aaagacaaca cgtatttgtg ccttttcact atcttgatta aaatgttttt
    23881 caatcttttt attcgtatct aacgactcta atcctgtcat tttaatatca ttgtcaaatt
    23941 tcggtgcatt tgtagcaagt ggtatcaata ttgcagctac aatcactatc catgcaatga
    24001 ccgcggacca tttatgtttt gcgatgaatg tccccatctt atataaaaat tttgccaaag
    24061 tatattgcct ccttttaaaa tcaacgttat agtttaaata tacagtgtag attattgttc
    24121 gattatagta tctatccccg acctcttaaa gaatcaattg gaaaattttg tatattaaac
    24181 tacacacaaa ggagaaatgt agatgaaaga gactgattta cgagttataa agacaaaaaa
    24241 agcattgtcg agtagcttgc tacaattgtt agaacagcaa ttattccaaa cgattactgt
    24301 caatcaaatt tgcgacaacg cactcgtaca ccgtacaaca ttttataaac atttttatga
    24361 taaatatgat cttctagagt acttgttcaa tcaattgact aaagactact ttgctagaga
    24421 tatcagtgac cgtcttaatc atccattcca aacgatgagt gatacgatta ataataaaga
    24481 ggatttgaga gaaatcgcag aattccaaga agaagacgct gaatttaata aagtattaaa
    24541 aaatgtctgc attaaaatta tgcataacga tatcaaaaat aatagagacc gtatcgatat
    24601 tgacagcgac atcccagata atctcatatt ttatatttat gactcgttga ttgaaggttt
    24661 tatacattgg ataaaagatg aaaaaattga ttggcctggc gaagatattg ataacatttt
    24721 ccatagatta atcaatatta agattaaata gtagatgaga aactcatgag cgttaccaac
    24781 attcataata aaaacgatag tgtacacgtt aatgaattcg tgtactacta tcgtttttta
    24841 tttttatcgt gcttatcgct attaaaacaa ctgatacaca acacataaac tatgaagaaa
    24901 aaaataaatc cgctatctaa atgactttga ctcagttgtt taaatgacca aattgctaat
    24961 acaattccca ttattattga aataacgtat ctcacattct tatacctata atccttttct
    25021 aaaaatatgg ttgctattac ttaattttta aagttataaa taaaaagagc caaccgcaat
    25081 ggatggccct tgttcattat gaagcattag aacatttctg aaacaacctt ttgttctaag
    25141 aagtgtaata agtagtctgg actacctgtt ttagcgtccg tacctgacat tttgaaacca
    25201 ccaaatggat ggtatccaac aactgctgaa gtacagcctc tgttaaggta taaattgcct
    25261 acatcaaatt cgtttaccgc tttaatccaa tgctcgcgat tatttgtaat cactgcacca
    25321 gttaaaccgt aatctgtatc atttgcaacc tcaattgctt catcaaaatc gttaactttc
    25381 acaaagccaa caactggacc aaaaatttct tcttgcatga ttctatcttt agatttaagt
    25441 cctgaaatga ttgttggttc tacaaagtaa ccttttgaat catcagtgcc gccaccttgt
    25501 tctaatttac cttcttcttt accaatctca atataatttt taatcttatc aaattgtttt
    25561 ttattaataa ctgggcccat atacgtattg tctacagtat tgcccaacgt taattctttt
    25621 gttaatttga ttgatttctc taatacttcg tcataaacgt ctttatgcac aattgcacgt
    25681 gaacatgctg aacatttttg accagaaaaa ccaaatgctg acgttacaat agcttctgct
    25741 gccatatctg tatcaatatt ttcatcaact acaatggcat ctttaccacc catttcagcg
    25801 ataacacgtt tcaagaagtt ttgaccttct tgaacaacgg cactacgttc ataaattcta
    25861 gtacctgtcg cacgtgatcc tgtaaatgta acgaaatgcg tatctttatg atcaactaag
    25921 taatcaccaa tttctttcgg atcaccagga acaaagttaa ctacgccttt tggtaatcct
    25981 gcttcttcta aaatttccat taatttataa gcgatataag gtgtatcctc agcaggtttc
    26041 aataacactg tattacctgc cacaactggt gctaaagttg taccagccat aatcgcaaac
    26101 gggaagttcc acggcggaat tgtaacacct gtaccaattg atttatagaa atatttattg
    26161 tgttcacctt cacgatcaag tactggctta ccttgagcca agtccatcat tgaacgtgca
    26221 tagtattcaa taaaatcaat accttcagct gcatcaccaa ctgcttcatc ccatggctta
    26281 cctgcttcat aaaccataat tgctgcaatt tccgcttttc gacgacgaat aattgccgaa
    26341 acacgtaaca taagctctgc acgatcattt gctgaccatg ttttccaaga tttataagct
    26401 tcgtttgctg ctttaaacgc atcttcaaca tcttgttttg ttgcctttga tgcatttgca
    26461 atcacttgtg atgtgtctgc aggattgatt gatttaattt tgtcatcttt gaaaatcttc
    26521 tctccattaa tcactaatgg tatgtcttga cctaattctt tttccacgtc tttcaatgct
    26581 ttcttaaaca tatccacatt ttcttggact gaaaaatcgt aaccaggttc atttttaaat
    26641 tctactacca tgtacactta ccccctataa attttgaaag tggtttaacc ctttgattta
    26701 atgatataac atcatttaaa ctcattttac tatgattaag gttagttttg caatcgcttt
    26761 catttttatg ttttatcact tattctcaag tattttgaaa ttgattggtt actttttaaa
    26821 atttatatgg gtcgcaactg ctactttatc gtttcgtcat ttaatgtttc ggatggtagg
    26881 tcattatcaa ttttacgaac gactttacaa gggtttccaa ccgctaagct gtgtggcgga
    26941 atatctttag tgacaacact accagcacca atcacactgc cttctccaat cgtcacccct
    27001 ggtaacacgg ctacatgacc gccaaaccaa gtattactgc caatatgaat gggtccggct
    27061 ttttcaaaac cttcatttct atgatggaaa ttaagtggat gtgtcgctgt gtagaatcca
    27121 caattaggtc ctataaaaac attatcgcca attgtaatct gtccaccatc cataaagtaa
    27181 caattggtgt ttacatagac attttttcct agtttaacgt tccaaccata atctgtatca
    27241 aaaggaatcg aaatacttac attgtctgtt gttgtttgaa ataattgatc aattaattcc
    27301 tttcttttat ttgtagcact cggtcttgta tgatttaatt caaagcaaat atctttcgct
    27361 cgtgcacgtt cattgattaa gtattgatca aagtttgcat cgtaccattt ttctgctaac
    27421 attttttctt tttcagtcat tacacctttc aactcctaat aacttattta cttgtttaaa
    27481 agttaatcaa ataaaccttc gcctatgcaa ctaatacgct ataacattat gaaatcatga
    27541 ccttatcacc cttatctata caattctcgc atcaaatact gctaaagtag tagataaatt
    27601 caatactaca gacgcattca ttttttaatc tattaacgta caatgtgagt aagagaaata
    27661 taaaggagta tgatagcgat gagaatatta attacaggca cagttgctat cttaatcatt
    27721 ctaggtttgg tcaaaacgat acaagattac gaaatgacaa acgacacgag tcgtcagttg
    27781 tcagacaaca aagatgatga taaagtcatc catcttaata attttaaaaa tttacatgcg
    27841 aaagaattta acccatctga tttcttttaa gtcacctaag aattgcaaat ccagaagtca
    27901 tttaagtttt acctttcatt catacatcct ttaatattaa ttacgacttc ttttatatag
    27961 atgctaagta gagagattgt tgtgcaatgt ttgcacggca atctctcttt ttctttttaa
    28021 aattggtaaa agtaaaacgc aacgattgac ttatatacct atagggggta cattagacgt
    28081 gtaacaatga atcacaggga ggcaataatg tggctaatac gaaaaaaaca acattagata
    28141 tcactggtat gacttgtgcc gcatgttcaa atcgtatcga aaagaaactg aataaacttg
    28201 atgacgttaa tgcccaagtg aatttaacta cagagaaagc aactgttgag tataaccctg
    28261 atcaacatga tgtccaagaa tttattaata cgattcaaca tttaggttac ggtgtcgctg
    28321 tagaaactgt cgaattagac attacaggta tgacttgtgc tgcatgctca agccgtattg
    28381 aaaaagtgtt aaataaaatg gacggcgttc aaaatgcaac ggtcaattta acaacagagc
    28441 aagctaaagt tgactattat cctgaagaaa cagatgctga taaacttgtc actcgcattc
    28501 aaaaattagg ttatgacgcg tctattaaag ataacaataa agatcaaacg tcacgcaaag
    28561 ctgaagcgct acaacataaa ttgattaagc ttatcatatc agcagtatta tctttaccac
    28621 tattaatgtt aatgtttgta catcttttca atatgcatat accagcacta tttacgaatc
    28681 catggttcca atttatttta gctacacctg tacaatttat tattggatgg caattttatg
    28741 taggtgctta taaaaactta agaaatggtg gcgccaatat ggatgtactt gttgctgttg
    28801 gtacaagtgc agcatatttt tacagtattt atgaaatggt tcgttggcta aatggctcaa
    28861 caacgcaacc gcatttatac tttgaaacaa gcgccgtact aattacctta atcttattcg
    28921 gtaagtattt agaagctaga gcgaagtctc aaacaaccaa tgcgcttggc gaattattaa
    28981 gtttacaagc taaagaagca cgcattttaa aagatggtaa tgaagtgatg attcctctaa
    29041 atgaagtaca tgttggagat acacttatcg ttaaaccagg tgaaaagata cctgttgatg
    29101 gcaaaattat taaaggtatg actgccatcg acgaatctat gttaacaggt gaatctatcc
    29161 ctgttgagaa gaatgttgat gatactgtaa ttggttcaac gatgaacaaa aacggtacta
    29221 ttactatgac agcaacaaaa gttggcgggg acactgcgtt ggcaaatatt attaaagttg
    29281 tcgaagaagc tcaaagttct aaagcgccga ttcaacgatt ggcagatatt atttctggtt
    29341 atttcgttcc tatcgttgtt ggtatcgcac tattaacatt tatcgtgtgg attactttag
    29401 ttacaccagg tacatttgaa cctgcacttg ttgcgagtat ttccgttctc gtcattgctt
    29461 gtccatgcgc attgggactt gctacaccaa cttctattat ggtaggtact ggtcgcgctg
    29521 ctgaaaatgg tattttattt aaaggtggcg agtttgttga acgcacacat caaattgata
    29581 ccatcgtttt agataagacg ggtaccatta caaatggtcg tccagtcgtg acagattatc
    29641 atggtgacaa tcaaacgcta caactacttg ctactgctga aaaagattct gaacacccat
    29701 tggcagaagc cattgtcaat tatgcaaaag aaaagcaatt aatattaact gagacaacaa
    29761 catttaaagc agtacctggc catggtattg aagcaacgat tgatcatcac tatatattgg
    29821 ttggtaaccg taaattaatg gctgacaatg atattagctt gcctaagcat atttctgatg
    29881 atttaacaca ttatgaacga gatggtaaaa ctgctatgct cattgctgtt aattattcat
    29941 taactggtat catcgcagtg gcagatactg tcaaagatca tgccaaagat gctataaaac
    30001 aattgcatga tatgggcatt gaagttgcca tgttaactgg cgataataaa aacactgctc
    30061 aagccattgc aaaacaagta ggcatagata ctgttattgc agatatttta ccagaagaaa
    30121 aagctgcaca aattgcgaaa ctacagcaac aaggtaagaa ggttgcgatg gttggtgacg
    30181 gtgtaaatga tgcacctgca ttagttaaag ctgatatcgg tatcgccatt ggtacaggta
    30241 cagaagttgc cattgaagca gctgatatta ctattcttgg tggcgacttg atgcttattc
    30301 ctaaagccat ttatgcaagt aaagcaacca ttcgtaatat tcgtcaaaat ctattttggg
    30361 cattcggcta taatattgcc ggtatcccta tagctgcatt gggcttactt gcgccatggg
    30421 ttgctggtgc tgcaatggca ctaagttcag taagtgttgt cacaaacgca cttagattga
    30481 aaaagatgcg attagaacca cgccgtaaag atgcctagat tccttaataa tgaaggattc
    30541 gttggtgatt ctgagatagg ctagtgattg gctctataat gtcgcggttt acagttggat
    30601 cttcgctcca actgcataag agccactaat tattataaat aataagtaca ctacggttta
    30661 cagttggatc ttcgctccaa ctgcataaga gccactaatt attataaata ataagtacac
    30721 tacggtttac agttggatct tcgctccaac tgcataagag ccactaatta ttataaataa
    30781 taagtggctc taaaaggagg tgaatgctat gtcacaagaa attttaaatg ttgaaggtat
    30841 gagctgtggt cactgcaaaa gtgctgttga atctgcatta aataatattg acggtgtcac
    30901 ttcagctgac gttaaccttg aaaatggtca agtaagtgtt caatatgatg acagtaaagt
    30961 tgctgtatct caaatgaaag acgcaattga agatcaaggt tacgatgtcg tttaattagg
    31021 caatattcaa cgtcatcaac accaaattaa aaaatcgaac tgatgagaat cccaacaatc
    31081 caaattatct catcagttcg atttttaatt tactcgtaac ctagtatctc cagtctgcaa
    31141 tacatctaat gttgcatcta atgcatcgac aattagattt ttaactgcag cttcagtata
    31201 aaacgcaata tgtggtgtta atatgacatc ttccctgtca atcaacgatt ctaacaatgg
    31261 atcgttcagt gttttgcccc tttgatcact tgggaaaagt ttgcgttcaa attcatacgt
    31321 atcaagtgct gcacctttaa tcacaccatt gtctaatgcg tctaataacg ccttagtatc
    31381 tactaaagaa cctctcgcac aattgacaaa tactgcgccc tttttaaaat gtttaaataa
    31441 ttcagcatta aatagataat gattatattt cgttgcaggt acatgtaatg tcacgatatc
    31501 agcaccttca accgcttcct caatcgtatc tttgtaatcg acatacgttg caattttagc
    31561 attaggaaac gggtcgtatg cgaccacatc actttgataa ccattggcaa atatatcggc
    31621 tactacacgg ccaattcgac ctgtaccaat aacagctact tttaaatctt taatggattt
    31681 cgataaaata gtaggttccc atctaaaatc atgctcccgc actttcgttt gaatttgatt
    31741 aaaatgacga accacattaa tagcctggtt cacagcaaac tccgcaattg aattcggaga
    31801 gtatgacggc acatttgaca caataaagtt atacttgttt gctaactcca aatcatatgt
    31861 atcaaatcca gcactacgtt gtgcgatttg tttaatacct agttcattta atcgtttata
    31921 aacatgctct gataatggta tttgttgtga tagcgataag ccatcataac cagcgacacc
    31981 ttcaacattg tcatcagtta atgcttcttt agtaatatct acctcaacat gatgtttctc
    32041 tgcccacgcc ttgatataag gcatatcttc atcacgtaca ctcatgattt taatttttgt
    32101 cattttaaca tcacccttaa ctttattatt catataaata tgctagttct gttaatctta
    32161 ttgcagcttc gtctaatttc tggtcatcta acgccaatga aattctcaca taacgattac
    32221 cattctctcc aaatggtttc cctggagcaa caagtattga cttctcttgc actaaaaatt
    32281 gctcaaattg ctcgctgtca taaccaggcg gtgtttccaa ccatacatat atgccacctt
    32341 tagcatgaac aaatggcaaa tcagcttttg caagcatggc ttcgaatcgg tcacgacgtg
    32401 ttttaaatac attgctttgt tcttctaaaa aatcatcata atgattcaaa gcatatattg
    32461 cggcatcttg taatgcacca aacatcccag catttgtgtg cgtttggtac tttttcaaag
    32521 cttgaatcat atctttatta ccaactgcaa aaccgactct aaaacctgac atgttataac
    32581 ctttagacaa ggaataaatt tcaatcgcaa catctttccc attttccgaa gcaagtatac
    32641 taggattttt agcgtcgaaa ccgaaagcac cataagcaaa atcatgcacg attttagtgt
    32701 ctgtaccttt aaatttagct atcgcttcat caaaaacttc tttcgtagct gtcgatccag
    32761 ttggattatt tggatacgtt aaataaatga gttttgtttt atctattatt tgtgaatcaa
    32821 ctttggacca atctggcaaa taatgtggcg gttctaaatt aagcgggact ggcttgccat
    32881 cagctaaaag tacacctgct aaataatccg tgtagcctgg atcaggtagt aatacatagt
    32941 ctcctggatt gataacacat gttggtactg ccactaatcc attttttgta ccatataaaa
    33001 tgcatacttc atcttcttta tctaacgtca cattatattg tctttgataa aaatctacaa
    33061 tagcttgctt gaacgcttct ttaccatgaa aagcaccata tttttgattt tcaggaatag
    33121 ttagtgcttt ttgaaaatga tcaataatac cttgtggcgt gggcccatca gggattccaa
    33181 ctgccatatt aattaatggc aatggtccat gttcgatttt acgtcccatc gttttcccga
    33241 aataactatc agggatattt gctaatttgt tagagatcat caaattcctc ctctatcatt
    33301 aaacatagcc tgggcgacta tcataatcct aacaacttgt atcactctca tttagatggt
    33361 tacaatgaca tcgccattca ccgttatgtt caacagaact tatgacacac gttgtattga
    33421 atgaatttat tttcatttta ggtaggtata atatcattgt caatattagg aattttcaga
    33481 ttaatatgca ctcaatcgtt atgatttaac tgtcatgcat atccgcatgc gcaaccagtt
    33541 agatatgctt atataaagta taacgcccat caaggtacgt attcaaacgt gaaccttaac
    33601 aggcgtcatt cattgttaaa taaaacttct taagcacata cttatttcac tatgcctttt
    33661 acgttcccct tatacttttc tcacatcttt ctcttagact actcccttat acgccccgct
    33721 caatatcttt aatcatttca tctacagtta ttttcgcact cgttaagaca ataggaacgc
    33781 ctgcacctgg atgcgtactt gcacctgcaa aatataaatc tttataatct cgcgatacat
    33841 tttgtggacg ataataatta ctttgcgcta aagttggcat taaaccgaat gccgaaccaa
    33901 atttcgcatg atacgtttgc tcaaaatcat ttggcgtaaa gattgtttct gaaacaatat
    33961 gcgattttat atcttcaaat acttcaatcg ttgctaattt acgataaata atttccttta
    34021 tttgttgcgt caaagcttca tctgaccaat cgattccgct acctgtttta agttccggcg
    34081 tcggcattag cacataaata ccagttttgc cttctggcgc aagtgattta tcagcgaccg
    34141 ctggtacata cacataaata gaaggatcat atgataaacg tccctcaaat atttcttcaa
    34201 tattgcctct aaagtcatct gaaaaaataa cattatgaag tctcacttga tctgtcacat
    34261 caatatctat accgatatac attaaaaatg ctgaacaaga gtaatctaag tctgcaattt
    34321 tatgtggtgg atacttttta ataggtgcaa aatctggcat taatgattcc gcaacactag
    34381 ggaaatcagc cgtacataaa attttatcaa attttcttat gtcaccattc acttttatcg
    34441 catcggcccg tttgaattta ggatcaataa taatttgctc aatttcagca tttagttcaa
    34501 tattaacgcc taagtcttta tttaattgcg ctagcccttg agccatgcca tacataccgc
    34561 ctttaataaa atgcacacca aacatcattt caatcatagg aataattgaa tatagtgacg
    34621 ggcctcgttt tggatcaatt cctatgtata acgtttgaaa cgctaaaagc ttttgtatct
    34681 tttcgttatc aatataatgt tcaattagct gatctgcatg atttaacgtt tttaacttag
    34741 caccttgcac aagtgacgtc atattataaa agtcactcgg tttgcgatac gttctttcta
    34801 agaaatagcg acgtgcaatt tcatattttt tataaacatc cgttaaaaag gacataaaac
    34861 catgcgttga accaggttct atactttcta gcatttgctg taattcagct aaatctgtag
    34921 gcaccgttat acgatcatcg tggtcaaaat acacatcgta aatataacgt aattgtctca
    34981 attcaatata atcttcataa tttttaccac acgctgtaaa aacatcttta taaacatctg
    35041 gcatcatgac aattgtggga cccatatcaa atgtaaagcc gtctttcttt aattgattca
    35101 tacgcccgcc tacattatta tttttttcaa atatcgtcac ttcatgacct tgagaagcaa
    35161 tacgggctgc cgctgctaat cctgtgacac ctgcaccaat tactgcaatc ttcattattc
    35221 aaccacctat attctatgat atttactatt tatttcatga aacaactttg cctttttcct
    35281 cttatccaca aaaacacgtt catgtaatgt atagttagcc tgtctcactt cgtccagtat
    35341 ttcaatatat atacgtgctg ctaattctat gattggttgt gcttcaatac taaatacttt
    35401 gatttgatcc ataacatctt gaaaatcttt ttctgcgata gctgcataat attcccataa
    35461 gtcaatataa tgattattaa caccattttg gtacacttca gcaatatcaa cttcatattg
    35521 ctttaatcgt tgcttactaa aatatatccg ttcattgtca aaatcttcac cgacatctct
    35581 taatatatta atcaattgca acgattcacc aagtcttctt gcgacatcgt atgtctgatg
    35641 tgtttcatga tcacttaaaa tcggcgtcaa tacttcacct actgtaccag caacaccata
    35701 acaatatccg aataattcag cgtccgtttc aaacattgta aaatgttgat ctttatatac
    35761 agtatcaatg agattataaa aagattgaaa ggcgatattt ttatgttgtg caacatgctg
    35821 aagcgccatc atgattctac gatcactttg aaagtgatga tgttcatatg ggtatttttc
    35881 aatagattgt atatcttctt ttatttgatt taaaaattga atatcgccat aaacatctat
    35941 actgtcatca attttacgac acacagcata aattgcccaa accgcttttc tttgatcttc
    36001 tggtaacaag tcaaaagcgt aagaaaagct ttttgaatgt ttcttcatga ttttatgaca
    36061 atatttaaaa ttcatatcca tcattgtcat actagtcctc ctatattgaa atgccattac
    36121 cttgatttat tgttctttag atatagaata ttgacgacct ttccattcaa ctacaccata
    36181 acgatgcgtt tgtttccaag attggatgaa aattttagta aaaaacataa ataacaatgg
    36241 atgacatacc attaataaat tagaaaattg gccgaccctt cgatgcagat aaataaattg
    36301 tgtagtatag ctcaagtaaa gtgctaccat ttttcttaca gacatttggc gatattttaa
    36361 actaagacat agccctaaaa tactcgctat agaaccaaac aaccacaaca caattgctgt
    36421 catgatctta ggctttgtgc cacctgcccc agttgacaaa tgctttgtcc atccttcttg
    36481 taatgactga aatccttctt gatacatgcg aaatgcaaca aatggaaacc cttcataaac
    36541 tgttacgggc aatgattgtg aagtatatgc acttcctaaa gcaaatcctt caataatatg
    36601 acggtttgca cttttatgac ctccagttgc ataataatct tctttatttg ttaatgtcac
    36661 cggtccaaat gcatgctggt tagtccgacc gtcgtctaag gtagaaaata cattcatacc
    36721 aacgactgtc attaaattaa atatcgctga aaacccttcg tagaaacgct ttgttatatg
    36781 ataaggctgt acgcttaaca atcctttttc accttgtaat tgatactgat taatcaacgt
    36841 ttcaacagca tctttcctta agaaagttac atcagcatct acaaaggcaa tgcgattcgt
    36901 acatgcatgc gtcacacctt gataacaagc atgtgatttc ccataccatt tgccgtcagt
    36961 atcatcaaca acatctacta cagtcgcacc atatgaacgt gctacacgtg ctgtttcatc
    37021 tgtcgatccg tcattcataa caatgacatc tactggaact tgctgttgta ttatcgaatg
    37081 tagtaaatga cctattcttt tttcttcgtt tctagccgga ataataattg ttaatgcttt
    37141 atgattgaag ttcagcgttt tcgcctttag ctgatgtcta cgattaaata tcaatgcacc
    37201 acacgccata gacatggtca ctattactgt taatattcgt gataaccatt tcatagatat
    37261 cacttcctat tcttcgcttc tcgcgcgttt attttgtctg cgacttgttg cccacttaat
    37321 gtaaccattg gcatgccacc accaggattt actgatccac ctacaaagta caagttttca
    37381 aaatactgac tttctttagg aaatttaaat cctttgtttt tctttttatc tgctacaaca
    37441 ccatatattg caccacgatt agaacgataa tttttttcaa tatcctccgg tgtccaaaca
    37501 tcttcataaa taatgtgttt tcttaaatca gtaagtccca ttttttctaa tttatccaaa
    37561 attttatccc taaacttcgc ataatcttca gtggtaaaag gctgatcttg aatatatgga
    37621 atatgtggta agactttgat attttcataa cctactggcg cttgtgtgtg atcagtttta
    37681 ttcgtattta ctagataaat ggtcggatca tctggcaata ccttttcatg aaaaacttgt
    37741 tgataattga gataagcatt ttccgtaaaa aagaaattat gatgtgctaa ttgcgggtat
    37801 tggcaagcaa cacctaaatg catcacatat cctgagcttg ccggctcaaa ttccctctct
    37861 aatttgttta atcgttgagt atcaaggtga attaaatatt tataagtagg tatgacttcc
    37921 atatttgaaa taatataatc tgcctttaca aactcacctg tatctaatct gacacccgtc
    37981 acacgtcttt gatatgtttt aatattgtcc acacgtgcac ctgtatgaat tgtgacacct
    38041 tcttcacgcg ctagcttttc caaggcattg gctaaatgat ggattccacc ttctacatac
    38101 caaaggcctt gctcttgttg catatggaat aacatagata atacagctgg cgcatcgtat
    38161 gatgaagaac ctacatattt gataaaatag cctaacattt gtcgcaagta tggattcgag
    38221 atacgcttgt ctatggcctg ttgcatagta tgtacataat cataattaat aagagcattt
    38281 aatggcccat gaaatttaat aatttgagat agtgtatcta aaccatagtt gaaataccct
    38341 ttttcagtaa tacgatcgat tcgtcttgta taattcaaat aattttgcag ttcctctata
    38401 tcctgtttcg acaatatcgc attatgctga cctgtttctt taataccttc atacaaatcg
    38461 atagtcgttc catctggaaa aaagctacgc cattgatgtg gcaatcgctt gattgtaacg
    38521 tagtctgaca tttgcttctt gctatattcg aataattttt caaaaatata aggcatcgtt
    38581 aaaatagatg gacctaaatc aaagccaaag ccatctgatt catgacgatt cactttgcct
    38641 cctatatgat tattttgttc atataatgag accgaatagc cactttgtgc cattcgaatt
    38701 gctgcagaaa tcccacctaa gccaccacca ataacgatga tatgtttagt catgacgttc
    38761 acctcttcgt tttaatatgc gtagtaactg cgttaatctc ggtcgattat agcgttgcac
    38821 aataatgatt ggaacattag ctatcattgc atagaaaata tttatatact ttactaaacg
    38881 agggccttta ttgaatatga tgactggaag tatcgatatc caatgaatca actctgccct
    38941 ctttgtctct ataatcattt tttcaatttc atcagtattg attgtcatta aatgacgctg
    39001 atcatatata ttttgattaa gctgatgacc atctaaaatc ttatgtttcc attttcttac
    39061 gtaaaaccat ttgttccaaa attttccatg cttctcaaag ttaaatgatt taaatattat
    39121 gtacttctga cgaaaatact tatcaggaat tcttgtacct aaatttgcta tatttagttg
    39181 aacaattaac caatacatac tacaaaaaaa tgctgtttta atatattttt tcatggtttt
    39241 catctaaatt gaatcactct caatcatact gacatagtat aaattatcac tttattactt
    39301 agttccctaa agatcagtga ataaaccgct ctcatttata tttatataaa acaaaaacgc
    39361 caatactctc atgttagaga atattgacgt catttgattt acatttgatt aatgaatgaa
    39421 attatatgaa cctgcttggt ttgctgaaat tgtacgagac gtaaccacac cagcaccatg
    39481 tccatagttc atttctgaaa cacgaacaga accgttgctg ttaacgcctt caacgtaagc
    39541 aacatgaccg taatagcctt gtgttgtttg catgatagca ccaacttttg gtgtattgtt
    39601 cactgtatag ccagatgatg cagctgcgtt agcccaatta cttgcgttac cccatgttga
    39661 accaattttc ccaccaacac gatcaaatac ataataagta cattgtcctg aagtatataa
    39721 gttacttcct gatgcataac cattagaaat tgaacgacca tttgaagatg gcgctgcagt
    39781 tgtagtcaca tgaacattat tacttgttgt gctataactt gcacctgaac caccagtata
    39841 atagttattt gttgctgttt gactattgtt tgtatagtta ttgtattgat atgaatgatt
    39901 atagtagtta ttgtaagatg cattgttata actataagta ttgtagttgt tgtagtagta
    39961 tgtgttgttt tgcgttaatt gacttggatt ccaatttcct gtccaagtgt aatgataatt
    40021 accttgtgca tcaattgtat acgtatagct gtatgattga gcgtcattag aattatatcc
    40081 attgttattt tgttctgcgg cttgtgcatc atgtcctgca aatgcgatag tggcaagtcc
    40141 tgctgtagcg attgtagctg taacgatttt tttcataatt aaatatcctc ccaataatca
    40201 aaacactcta tacatatagt cattagcgac tcaattattt taaattttat attttttatt
    40261 tttttagtat gcaaacaatt ataactttta tagttttgcg acaaaacaaa ctataacaga
    40321 gattaggtgt cttgcgggct ttgttatcat aatgtaatct gttcctaata aagcttcatt
    40381 gaatgtaata taccaattag aatgtttaca acactttttc tgcaccgtta ctatgggatt
    40441 tgaaaataaa aaagcactaa acttttaatg aaatacatca tttttgataa taaatatcag
    40501 aagtaatgta atatttcaaa agtttagtgc atcaaattat ttcttatttg tagcatgtgt
    40561 ttccatcgtt tttacaatca aatcagtcag cgctttacta cctgcatatt ctaagtgaat
    40621 accgtcatat gcaaagtatt ccggatgacc tgcagaagct ttataccaat cgactagatg
    40681 tacattagag cgcttttcag ctgcctcata aattaattta tttattctac cttcataatc
    40741 tctaggtact ctaatagaaa ctaaatatat gtctgctttt ccaaaactat ccaatagttc
    40801 atttaattga tctttcgtaa atgccccatt tgtaccaagc tctactacaa ctttttgacc
    40861 ttttttagca tagtctttat attgcgattt cacaattggt gtagcatcaa cgagttgccg
    40921 tccaacttta ccatcaattt gtgcatttgg tattttctta gtaaagacat taccaatatc
    40981 caccatgacc gagtcaccaa ttaatagtgg tgatgacttt ttaatatcct ctttgctatt
    41041 cgctgtctgc ttatcctctt gcttatcttt tttaacgact tttttcttcg ttgttttaaa
    41101 ttccgtcttc ttctctttct tctcggcttc atgttgtttg ccaagtgcat caaactgtcc
    41161 actgagcaca acgatagacg gaaccaataa taggataact aacactgttc tagcaaattg
    41221 ccccttcttt ttaggtaaaa atgcaaaagc tttaaatcct tttttacgta taggtgtttc
    41281 aataaagcga tacgaaattt cagccattaa cgctgttaac aaaatttcta taatataaac
    41341 gtataccggt atttgtcctt gtacgtaata actgttcaca aaaacaatga taggataatg
    41401 ccataagtat aagctatatg atcgtttacc tataattagt aaaggtttca tacttaaaaa
    41461 tttagcaaat aaactagaag gatgtaccgc aattgcaata atgaataaag ttgcaaatga
    41521 tataatgtaa aatcctccat tatagatcca ttgatcttgg tctccaacta taaagaacaa
    41581 agtcattaga accgcaaaac cagatatccc tataatatct aatgatacga caatcttttt
    41641 agaaatatct tttttcaaag caaacggagg ccaaataaat gctaatatac aaccaagcaa
    41701 taaagtttgc agtcgtgtat ctgtcccaaa atacacacgt gaattatctc cagtgatgaa
    41761 atgaatcact atcataagtc ctaaagaaat caacgataca ataaatagcg tttgaataat
    41821 atttctcggt ttaaatctat gtaataagaa cgtgataacc aatgggaaaa gcaagtaaaa
    41881 ttgttcttca atggctaaag accataaatg tttgagtggt tcaatagcaa attggttaaa
    41941 ataatctaca ttctgtgaaa tgtaccacca gtttgaaaca tagaatatag ctgcaatagc
    42001 atctcgtttc atttgtataa tcaattccgg tttaaatatc agtgtgaacg taagcacgac
    42061 acagattaaa aataacactg ccggaatgag tcgtttcaat cgtcgcttcc aaaactctag
    42121 caaatcgatt ttttgcgtcc gataatactc acttatcaac aaacttgtta ttaaataacc
    42181 tgaaataacg aagaatgtat ctactcctaa aaagccccca cttaaccatt gtgcattcaa
    42241 gtgataaata atgattccta taactgcgaa tgccctcaat ccatctaatc caggtaagta
    42301 tcgcggggaa tacatttttt ctaaacgttt aaagtctttt gtatccatgt taataaacgc
    42361 cccatttatt tttctctatt ttgtagcata tcacaatatt tttgaaaata aaatattgca
    42421 ctgattttca ttaattgatt taacccttaa ttaagatagt tttaaatttt ttattaagta
    42481 gaaaacaatt attacagttg atttcattac tgcaaaccac atataaattt gtcgatttta
    42541 ctacataaca tagattatca tagattcttg aatttttagc aaaataactg ttattttcat
    42601 tatattttta caaaaaaggt tcgttttata ttttatgcat attactgtaa cagaatcatt
    42661 aagatatgct attcgaatat actttttcaa aatttatata atgaataaat taacatgtat
    42721 tgaaaaaaaa gcgaaatgca gcctatcctc taatgtaaac caaacgatat atctcgtcag
    42781 actttatatt taaacgctat gtgtcacttt taaaatgaat attactaaga ttgtcatatc
    42841 aattattatt gcatcgaatt aatcttttaa atttctgtaa taaaacctat tatctacatc
    42901 taatttagga aaactacttg gtaaatctcc tcgcttaatt tcacggaagc cattattaga
    42961 ataaaaatac tgtgcactaa taaatttatc aattgttcct aaataaatac catcgatatt
    43021 ttgttcttta catgtcatta taactttatc taaaagtttt ttacctattt ttaaattcct
    43081 ataaccttta tcaacaaaca tttttttaag tgcagacata ttattatcta gtctaatcaa
    43141 acctatagta ccaacaatat tttgatgatt gtttattgca agccaaaatt gccctccatt
    43201 attcaaatag ttatgttcga tgttctccaa atcaggttga tcatctctat caatttttat
    43261 attaaattca tttttttgaa tcgataaaat aaactcgatt agctcttcct tataagacct
    43321 attatattca attatgttta tagccatttt tatctccttt ttcatttaat ttaattataa
    43381 aatgtgcgtt tagtttgtat ctagtgtact cagtacagcc tcaaatgaag tttcattcca
    43441 cttggcactt aataaagaca agtattttag cagtaataca ataaagtcca ataaatttcc
    43501 ctaacttcaa tatccacttt ttaaaaaatg tatttttaat taataaaaaa actctcccca
    43561 atttctatgg gaagagctat atatttaatg tctaaacatt acttttattt attatgaagg
    43621 aattagaatc cccaagcacc taaaccttgt gctttgtatg ctttaacagc tgcgttgatt
    43681 tgttggtcaa cagtgtttgt tggaccccaa cctggcatag tttggaataa acctgaagca
    43741 cctgatgggt tgtaagcatt tacttgacca tttgattcac gagcgatgat tgcagcccat
    43801 gtagaagctg aaacaccagt acgttgagcc atgatttgag ctgctgatga accagtagca
    43861 cctgcagtat taccattgct taatctcact gaacttgaag tagttgaagt gctgtagtta
    43921 tggtaagttg gagctgaaac agcttcaacg tttgagttac ttgattgtgc attgtagctt
    43981 attgattgta catttgaacc ttggttgtat gaagtagtgt agtctgcacc tgcaacgttt
    44041 gagaaaccag cagtttgacc attagctgct tcatagctcc atgaccatgt agtaccattt
    44101 gaagtgaagt tatattggaa accatctttt acaaagtgga tgtcatatgc accatctttg
    44161 attggagctg catttaattg atcttggtga ttatgcgcta agtcaactaa gtgtgcttga
    44221 tcaacgttta cttcagcagc gtgtgcttga tgtcctgtac ctgctgcgta acctgttaca
    44281 cctaatgcca ctgctaatga tgatgccata attgtctttt tcatagtaaa aaatcctcca
    44341 gtaataattg taagtttatg tttttagtaa ttatattttg aatttgaatg tcgtagtgca
    44401 agtttaaatt gtcttttatt tctttcaacg gtactcacta tatcacaaaa aaccagccag
    44461 taaattacac tttctttaca aaacattaca atatcaagtg ttatttgtaa tgttgaaata
    44521 tggctgtttt atactgtaat gtgaaatatg tgccctttag aatccaatca acccttgaaa
    44581 tagtctttaa cacataagat ttttactata tttagctcaa ctattacagc tttcgtaata
    44641 ttacagattg tatttttgtt acatagctgt aatatatctg acatgtaact tctcttattt
    44701 tcgtacatta aacgcaatta aaaagcaatc aacaaatatg tttctacaca tgtattgatt
    44761 gctattattg ttgtatattc aaagttttaa aacacacatc ttttgtgaat tgtcttatct
    44821 tttattagcg caaataaact gcagctcaat tatattgttc aacttcattc tcgcaattca
    44881 caataacatt aaataatttt tggtctcata ttttcaaaaa acatactgtt attatcccat
    44941 gaatttaaaa atatcattag tatataaacg aaacacttta cgataaatga tatctgcaag
    45001 ccaagctgtt acaaatggta caacaaagaa cgctactaca attagtaaga cactcaacca
    45061 agcagaatca acctccataa atttaaatgc attaatcggt cctaccattc ctataaaacc
    45121 aaatccagct gactctttcg ttccatgaat acctactaat gctgatacca aacctgatac
    45181 aatggctgtc gttaatattg gtaacataag aattggatat ttcaccatat taggtatcat
    45241 cattttaacg cctccaaaga agacggataa cggcacccct aaacgattca ctttacttgt
    45301 accaattatc aatactgctt cagtcgcgga gataccaatt gacgctgatc cagctgctaa
    45361 acctgtaata cctatcgcaa aggcaatggc cacagttgat agtggcgaaa taataataag
    45421 actaaatacc attgaaatca aaatactcat gacaatcggt tgtaattctg taaaaccatt
    45481 aaccatatta ccgatggctg ttgtaatcat tttcgtatac ggcaatatta aaacaccaat
    45541 tgcacctgaa ataccgccaa caactgttgg gaatacaatc aatgccatac tacctacgcg
    45601 atgttgaata agtaaaatga ataacactgc aatcgctgct gtaatcattg tattaattaa
    45661 atcaccaata cccgtaatca tccaagcacc atttttaaac tgcgctgcac cgcttcctac
    45721 atatgctgca cttgccacaa cagcaattgc taatggcgat aggtcaaatt tcatggcaac
    45781 caatgcacca atcaaagcag gtactgtaaa ttgaattgca acgacaacgc ctaataacgt
    45841 tttaaaaatc ggatgataat ccataaagta tttaaaaatt tctccaagta tcgcattagg
    45901 aactaaaccc gcaacaatac ctatggcgac acctgataaa actctaaata taaaatcttt
    45961 gggtgtaatt gttttaattg atgtcataat atcatccttc catttatgta tatacatctg
    46021 tatgcaaata ataaagagcc ttaagttata agctgccact agcttaaatt ctaagatgtg
    46081 catgccgatg ttgttatatt taggctagca gtatcatcta taactcaaga ctatgaaaaa
    46141 tagtatatca caaaattctg aattttcaga taaagaaatt ggcaattttt caaacatatt
    46201 gttacaatac acttttattt tatcttcatt tttaaaatcc attaatacaa tagaagaaag
    46261 acattcaaat gcttaccaaa aaggtacatt atttgttagg agcgtatcag cacttacata
    46321 tcatcaacac aattgacaat ataatagaag atactgataa taagtgttaa aacaacagat
    46381 gttaggtagt gaacaaatga tggaaagtaa atccatagat ccaagaatcg ttagaaccaa
    46441 acaattgctt gtcgatgctt ttcttaaaat ttctagagaa aagaaattat ctcaaattac
    46501 agttaaagat atcactgata ttgctacgct aaatcgtgca acattttacg ctcatttcac
    46561 tgataaagaa gacctcctag actacacatt atctgtaacc attttaaaag acttgaatga
    46621 taatttgagc atttctaatg tcattaatga aaaggttctg cgtaatattt tcatttcaat
    46681 tgcgagttat atcaaagatg ctgcaaagtc ttgcgaatta aatagtgaag cattttgcaa
    46741 caaagcacat caacgtatta ataatgaatt agaagatatt tttgcgatta tgttagaaaa
    46801 cagctatccg gagcatcaac gagatatcat tgtaaatagt gcgagttttt tagcagctgg
    46861 tatctcaggc ttagcattac attggtttaa cacgagtcaa gagacagccg atgtgtttat
    46921 cgatcgcaac cttccatttt taattcatca tatagcacat ttttaataaa acttggtatt
    46981 tagtcatgca tcttgaaatc actatgtgac ttaggttcat acttgtacac acaataaaat
    47041 ttaacgtatt acgattgatt agccgtgtct aggacataaa tcaacgtcct atactctaca
    47101 atgtcatatt agcagtcgtt aactgaatga aaataagctt gtcattaaaa catatagatt
    47161 ttagtgacaa gcatttttgt ttttgcgtac ttaaacaaca cttcaggcaa tatgttgttt
    47221 aggcaacaaa tgatatgtgc gtgtttattg gcaaacgtac gacatagtag tatagtatgt
    47281 ctaaacaaca tatgttgcat agttgatatg cgttgtttaa atactaagat aggagggatt
    47341 gacgtgagcg agacagatga acctcagggg tttgaacgca cgcataatat attaaatatt
    47401 aatcagagta gtctgggtgt agtgacatac attacaaata aattaaagtc gacgttgaag
    47461 caacacataa taattgctcg tggtaaaaag cgaatcgact atcgactgtc gtataacttt
    47521 tacactacgt attatgataa tgtagaaatc aagaaaatcg actgtgaata tacctatgct
    47581 atgcccattg caattttaat aagacacacg atgtcattcg acaatgctca tttctttgct
    47641 cagttacgtc atcctgtctt ataaaacaac attgcagaca tgtatatcaa acgacacttc
    47701 aataacatca ctttgccatc gtactactag taaaatcgtg tctcaaatcc cttattttaa
    47761 ttccaaaaat ctgctggtca aaagaccgag aaactaaaaa cattacttaa tgtgttgata
    47821 aattaccata taaaaataat ctcaaaatat atcaacactt gattctaagg aggatatgac
    47881 aatatgaaaa ttttagatag aattaatgaa cttgcaaata aagaaaaagt acaaccactt
    47941 actgtagctg aaaaacaaga acaacatgca ttgcgtcaag actacttaag catgatccga
    48001 ggacaagtat taacaacatt ttccacaata aaagtggttg atccaatcgg tcaggatgtc
    48061 acaccagata aagtttatga tcttcgccaa caatacggtt atattcaaaa ttaatatttg
    48121 ctcacgaggt attgcactta aggtgccaac tgacctcata aacaaagccc atactgattg
    48181 aagacactaa tgtgtcgcac catggtgcac attacgcttc atctctgtat gggcttttta
    48241 tttattcttt tgagaatttc attttagcag accaaaaaat taaaatggtg tccatcaaga
    48301 tctttgaaca ttgctccata aaacccttgg cttactgttg gttcttgaac gactgtgcca
    48361 cccgcttctt tcactttatt caccaactgg tcaacttctt catttgtgtt cacacctaga
    48421 gaaatcatca cttcatttgg ttcagtatta aggctttgct gacttacatt ttgaaaatgc
    48481 ttgttttcta ttaaaattac ggttgtttga cctatttgaa tgccgaccat tttatctaac
    48541 atttgtgggt ttctatttat tttaaatcct aacgctttat aaaactgtgc gctcttttct
    48601 aaatcttgca catgcaaatt aaaccacatt gattgaatca tgattgcacc ccattcatta
    48661 cttattatag ttttggactt taagccaatc acttaatgat aatcttgttg gatttatttc
    48721 agccattaat tcaaagtcta cttcataacc tttttcttcc aaccattgct tttctgcaac
    48781 accactaaca aattctcctt ctataacagt agatttacct gtcacttcac taaaaattgt
    48841 tgctgcttca cttaatgtaa cttcatcgga accaatctct attgattgat gcgtaaagct
    48901 ttgtggatgt gcaaaaatat acgatgcaat tttagctata tcaatagaag aaatcattgt
    48961 gaattttata ttcggattaa taaattctgg taatgtaata cgttcatctt cgactttagc
    49021 aatgcgtaaa aaattatcca taaagaatga tggtttgata actgttgcat ttatattaga
    49081 ttccattaat ctattttcta tttttgctag tacttcaaag tgtgggccag ttcgatttcg
    49141 attaacccct cccgcagtac tatacacaat atgttgaata ttttcttgct cagctatttc
    49201 aattatcttc ataccttgtc ttaattcttc gctaacatca tctttaacga ttggctgaat
    49261 actgtataag ccatacttac ctttcatcgc tgattgcaaa ctaacattat cactcagatc
    49321 accttcaacg attgataaat gcggatgtcc tatgtctgaa agtttacgat tattcttatt
    49381 tctagttaat gcacttacat accatccatc ctctaacaac tgttttacaa ctgcattacc
    49441 ttgcttccct gttgcgccta ttactaaaat atctttcatt ttagatccac tcctcttttt
    49501 atcttaaaaa ccaatagtcc tctttttcac cttttaaata cgtatatggt tctggtgttt
    49561 gtccatttcc tttaatattg tcgtttatta catctctgac atcccatact aaatgaataa
    49621 atcgttgtaa ttgatgacta aaaatgccta gtggcgctgt catagctaat actttttcca
    49681 catcttctgt agtgacattc agtttttcag caatcatttg aaccgataag gtagatgctt
    49741 cgaaatgtgc ttgcaactct tcagctgtga tgcgtctttg ctctttattt aaagacatta
    49801 tcaacactct tttcttttat gatatttaat agttattgca aattcattgc taactttctt
    49861 aacacgtttt ataataaacg gagagttatc cgtttgtcaa ttttagagaa ttttaagtag
    49921 gtgtaatcat gcgaaaagat gcaaaagaga ataggcaacg aattgaagaa atagcgcaca
    49981 aactttttga tgaagaaggc gttgaaaaca ttagtatgaa tcgtattgct aaagaactag
    50041 gtatcggtat ggggacattg tatcgacatt tcaaagataa aagcgattta tgctactacg
    50101 tcatacaaag agaccttgat atttttatca ctcattttaa gcaaattaaa gatgactatc
    50161 attctaatta tgaggttatg caagtgtcgt tagattatct tttacaattt aaaatcgata
    50221 ataaggcact actgcaatgt attgaagcag gcaacaataa attacgtttt taccaaagcg
    50281 cattttatca agaattattt gatttttatt atgacttatt taaaagcgat gacgacactt
    50341 acacgaagtt caaaacagat atgctgttgc aatcattatc aactagtgta tttgccttcc
    50401 aaatagagca tcgccatatt tcaattgaag cgtacagaaa ttatttatta aacatttatt
    50461 tagatgaggt gggacgtaat gactaaaatt gtgttaatca caggaggcaa taaagggtta
    50521 ggctatgcaa gtgcagaggc acttaaagca ttgggttaca aagtttatat aggttctcgg
    50581 aatgatgtaa gaggacaaca agcatcacaa aaattaggtg ttcattatgt acaattagat
    50641 gttacaagtg actactcagt caaaaatgct tataacatga ttgctgaaaa agaaggccgt
    50701 cttgatatcc tcattaacaa tgcaggtata tctggtcaat tttcagcacc ttccaaattg
    50761 acacctcgtg acgttgaaga agtatatcaa acgaatgtat tcggtatcgt acgaatgatg
    50821 aatacattcg tccctctctt agaaaaatct gaacaacctg ttgtcgtcaa cgtatcaagt
    50881 ggtttaggtt catttggaat ggttacaaac cccgaaacag ctgaatctaa agtgaattca
    50941 ttagcttatt gttcgtctaa atcggcagta acaatgctaa cgctacaata cgctaaagga
    51001 ttacctaaca tgcaaatcaa tgctgctgat ccaggcgcta caaatactga tttagttggt
    51061 gattttagta ataattccaa acacgtttct gaaggaatca agccaatcat tcaattagca
    51121 acgattggtg cagatggccc aacaggtaca tttattaatg gcgatggaga gatgccttgg
    51181 tagatcaatt ttatgttaca aacattgaaa ttagaggtat ataaaaaatg cttctgcaat
    51241 agatgcaata aacatgcaat acaatatgga ggcgaagtaa atgaaaagta ttacgtttga
    51301 agaacattat gtcattgaag atattcaaaa agaaacgatg aatgcgatat cagcagatcc
    51361 taaaggtgta ccgatgaaag taatgttaga aggccttgaa aaaaagacag gttttacaaa
    51421 tgccgacgaa ttatcacatc atgatgaacg tatccaattt atgaataatc aagacgttca
    51481 aattcaagtc ttatcttatg gaaatggttc tccttcaaat ttggttggtc agaaagccat
    51541 tgaattatgt caaaaagcaa atgatcaatt ggcaaactat attgcacaat atcccaatcg
    51601 ctttgtaggc tttgcaactt tacctatcaa tgagcctgaa gccgcagcgc gagaattcga
    51661 acgttgcatc aatgatttag gattcaaagg tgcgctcatt atgggacgtg cacaagatgg
    51721 ttttcttgat caagacaaat atgacattat tttcaaaaca gctgaaaatt tagacgtacc
    51781 gatttatcta catcccgcgc cagttaacag tgacatttat caatcatact ataaaggaaa
    51841 ttatcctgaa gtaactgcgg caacatttgc ttgttttggt tatggttggc acattgatgt
    51901 cggcattcat gcaatacatc tagtattatc tggtattttt gatcgttatc caaagttaaa
    51961 tatgattatt ggacattggg gtgagtttat cccattcttc ttagaacgta tggatgaagc
    52021 tttattcgct gaacatttga accaccctgt aagctattac tttaaaaata atttttatat
    52081 cacaccgagt ggcatgttaa cgaagccaca gtttgattta gtcaagaaag aagtaggtat
    52141 tgatagaatt ctttatgctg ctgattatcc atatattgag cctgaaaaat taggtgtatt
    52201 tttagatgaa ctgggtttaa cagatgaaga aaaagagaaa ataagttata caaatggtgc
    52261 taaattatta ggtttatcac ctaacaatta acaaatggag gtcattgaat atggaaactt
    52321 tagaattaca aggcgctaaa ttacgatacc accaagtcgg acaaggaccc gtgctcatct
    52381 ttattcctgg tgcaaacggt accggagaca tttttctgcc tcttgcagaa cagttaaaag
    52441 accattttac tgttgtagcc gttgatcgtc gtgattatgg agaaagcgag ttaactgaac
    52501 cactccctga ttccgcttca aaccctgaca gtgattatcg tgtcaaacgc gacgcacagg
    52561 acattgccga acttgcaaag tcattaagtg atgaacctgt ctatatatta ggttcaagtt
    52621 caggttcaat cgttgcgatg catgtgttaa aagattaccc tgaagtcgtt aaaaagattg
    52681 catttcacga accaccaatt aatacatttt taccagacag tacttattgg aaagataaaa
    52741 atgatgacat tgtgcatcaa attttaacag aaggcttaga aaaaggcatg aaaacatttg
    52801 gcgaaacatt aaatatcgca ccaattgatg caaaaatgat gtctcaacct gctgatactg
    52861 aagaaggacg tatagaacag tacaaacgta cgatgttctg gttagaattt gaaattagac
    52921 aatatacgca ttcaaatatt actttggatg atttcacaaa atacagcgat aaaatcaccc
    52981 tactaaatgg gacagattct agaggctcct tcccacaaga tgttaacttt tatataaata
    53041 aagaaactgg catacctatt gtagatattc caggcggtca cttaggttat attcaaaaac
    53101 ctgaaggctt tgctgatgta ttattaaata tgtggggtta attgattgtt ctctaataaa
    53161 acagcttgga cagttttcaa attatatttg atggctgacc aagcttattt ttatttacgc
    53221 ttaaacgttc aaatcaactc gttattatac atgaaggcat gttaaccatt ctcttccact
    53281 gaaaacacta ctgttccaaa cattgatagt cttcttttga aatacccttt ccgataagca
    53341 ccaaatattc tggcactttt tttgaaaatg ctacaggtgt taattcatat tgtccttgtg
    53401 taaattgaat gagatacgta tgtgcggtgt cttcaaatgt cataaaccct ttcaacctat
    53461 aaatatgaga cggaaggcat gctaaacgtt ttataaactc tgctttagta catttgactg
    53521 gtgattgcac aaattgatga ttaatcattt gatgtaaaga cttatgttta ttgtcagaag
    53581 ttgctgtcat ttgtctaact gatattggca aagtgacgct gccatgcata ccgacttgta
    53641 tatcggcctc tgggttaata acttctaaat ctttcaatag tttgctcgtt gtttcaacat
    53701 ctgctgaatc tattttatta acaaataaga cagaacaata tgctaattgc tcataaaata
    53761 agccttggat gtctttaggg aatgatttaa tgtgtttata catgcttgcg tctattacac
    53821 caatcatatg tgtaattgtt gtaaacggag ctaaaatagg cgttaaacaa gcatctaaga
    53881 cagagaccgg ttctgcaatc ccactacatt caataaatac aatgtctggt tgctctttta
    53941 aatataattg atgtaactgt tctgatacat ctgctttcat tgcacaacag atacacccct
    54001 ctgtcagttc acttaaaggt atgtcttcag atactaaggc accatcaaca ctcattttcc
    54061 caaattcatt catgatgact gctggtttca gcgattcctt taatgattca gcgagcaaat
    54121 gatttaagac agtcgtttta ccgccaccta aaaacccacc tataataact attttcatct
    54181 gcgactctcc tcttatactt catatgaata gcatattcca agatagatct cctattgact
    54241 gacatgactc tatattcagt tatagttcat tttttttaaa taatcagcat tactatttta
    54301 acaatgatgc ctacaccaat aactaaagac aatgatgtca ccatttgctt tccaattaat
    54361 tttagtgctg actgaccacc taaatgtttc aaaagcatcg tcattgtgac cgagcacgct
    54421 gtaaatgttg aactaaaaaa cacaagtagt agtaactgtg ttgctgtcat tccttgtaat
    54481 aaggcgccct gatgcaaatt aaacaagagc atgccgtctt ttcgaatcat tgaaaataaa
    54541 atccctggtg acaattctga cgagatgcct aataacgata atataggtgt aaatatttgt
    54601 gataaaacat tcaaaattgg cgttagtgat aaaatactaa caataagaca gattgtgata
    54661 aaaataggta gcgcttgaac gataaacatt tgtatatttt gccacatttg tagcaacatt
    54721 tgacgtatat ttggcatatg taattgccta tcataaggta gcggaacgct aagttgttga
    54781 tcattctttt tcaaccatat cctattatgt aagatgccac ctaaaagtac taatattaag
    54841 tacggcataa atagccatga ctttccagct acactaaaaa tagataatgt cgcacctatt
    54901 tgataactac aagaactacc aaagcttatt aaactcatac actgtgtctt cgtgcaggta
    54961 tggcattggt gtgctgcttg tgaaatagct gctgcattac atccaaaccc ttctaatagt
    55021 ggcacgatat cattaccttg taatcctatc cataacattg acggttcaat tgcccatatc
    55081 atccatgatt tgagtcctgt ttgatcaatt atagcagttg ataaactaat caatattaca
    55141 accggcaatg cccatacgag cgaatatgtt ccaagtgata gcacaccata atcaccaaat
    55201 aatatatgct gtaatatatc gtgattgagc aatgttgttt gagttaaata ttctatccat
    55261 gctgacacat actgattttg aaaccaatct gaaaattgat aagcgatacc gattggcaat
    55321 gcaaacatta aaaatatcaa caaccaggct aaaaatgtcg cacttatttt aggtcttaaa
    55381 tgtcgtcgct catgttgaaa tagtgtcaat tgttctatca catgttcact cgctataggc
    55441 gaagaaacta cactttctgc caatgaaata tctaatttct gttttatgat taataatctt
    55501 aatttcgctt tttctgttgc ggtaagttta tccgcatgac taataaccaa ataatgaggt
    55561 tcattaggta tgggcgtata atgcccagtt ctcatatcat atttcacttc taacatgtgc
    55621 atgacacttc acaacatgaa taatcatcta aatacatttg atttttttga taattttcaa
    55681 tgacatcttg tttagctggt aagccttccc gctgtgttaa aagatgtgca agtactgcaa
    55741 atcgcgctct aaatttatag atatagcata atttggcatt atcattttca actgttgcac
    55801 caatcataaa aatattcgga taacgtgtcg attcatcatg tgttgttaat ttaatatctt
    55861 gatttgttgt cacaaataat tgttgaacga ttggattttt tgttgcatca aagccagttg
    55921 ctagtattgg ttcatgaggt gtaagcacac tttgtccgct atcaaaactg atatgatact
    55981 gtccattgtt aaaatcaata tctttaactg tataatgtac attcatttcg atgcgagcac
    56041 cttgcttaat gacattacct agtcgctgac gtgtataagg tgacaatcta acactaggat
    56101 cagcatccgg atcatttaaa ccggttgtgc tagtataaag tgcgatgtca gagccatttt
    56161 ttgcaagttg atatgcagca tcaaagccac tttcattacc tccgataacc acatatttcc
    56221 ccttattaaa gttatcaaag tcttcaattt cactataatg aataccatat ttaaatggct
    56281 ttttagggaa attataatca cctgttgcga caaagatata atccgcgtga tatatctctg
    56341 ttgtcgttgc aatcgtataa tatgcatcat ctacagatat atttgtgaca actgtatttt
    56401 caaagatatt cagctcgtaa tggttggcaa ccacttgtaa atattcagca tatgtttctc
    56461 cggaaatatg ttcttcatta aatgtaaatg ctggtgaagt atccatggaa attgcattca
    56521 tatcaggcat gccaaatcca ttagacgtaa atgatggcgt gatcgtacgg gtcgatttcg
    56581 gccaatgttt aaatgaatgt cctactgttc ctttttctaa aataatgaca tctgttatac
    56641 cgaaatcttt taaggtaatg gccataccta tacctgcagc accggcaccg ataatagcca
    56701 ctttatgatg ttgcatatta acactccctc aatgatctat ttcaagttat tgtttttgat
    56761 tataaatcgt aataattacg atttataaag tatagaccta tccttgagaa atttcaatat
    56821 taaaagaaaa acttccgtta ttgtagagaa ttcatctaca tttcggaagt tggtatgtgt
    56881 ctttttttaa aatttaaggc tgctacgttt agttcaaatg attatgaatg acatttcatg
    56941 gtatcatcca gcctcagtac ttggagataa cattttggcg gtgagacaga aatcatattt
    57001 ttacaaaatt tatttcgtcg tcccacccca ataaagaaaa agtatttctt tattgttgtg
    57061 taacttctcc acgttgtatt tcactatcca aattttgttt catattttca aacaactctt
    57121 gaaatctaac ctcatcttga tcagaataac ctttaaaaat aactgaatct atattttcct
    57181 cgttaaaata ataaatttca tcttctacaa caccaatcgg atatttacat gcagaatagt
    57241 caaacatata cttttgattt tcaatttcta caattggtcc tctattaata atcattaact
    57301 tttgcgaacc ttcttttaaa taaataatgc ttcctattgt ttccatttat tttcctcctt
    57361 tgtcaccgtc tcaatttcaa cattccttcg catttccaag tcaatcatct cttcttccac
    57421 tttacgaatg ccatgttgaa tttgctgttg ctgctcgtat aacgcttccg ttgaacgtcg
    57481 tttcgcaaaa tgaaattcat cgcggaactg ttccatacga ctagccatat tgctactata
    57541 ataacgctca cctaaatgtt gatataattg atctatcgaa tgattaagcg catcactttc
    57601 atctatcaat gctttaacct ctcgcttttc atcatataag tcatccattt gctcatgttt
    57661 cgctgtgatt attttattga tttcatccag tttattcgac atcatttcac cacgttttaa
    57721 tttttaccaa tttcattacc aatcattacg tcagtttggt caaaagcctc accaactttt
    57781 tgtatattat cgctcgcagt tcgtattgct tcagcaacag attgattctt actcatcaaa
    57841 tcatctatca cactatgagc attattattt ccacttactg ttgtactttc atctttatcc
    57901 ggtttcgaca cattattaca tgtttcaaca gcgcttttaa tagatgatga tgcactattg
    57961 gtcttaaaaa tatcactttg aactgtactc atcatttaca cctctctcca tttaaattat
    58021 tttaaaatta ttagaaactg tatcaatata tgatattcaa acattcaatt atacttaaat
    58081 attgttttat gattatattc aaaattattg tatcaataaa tatgtatcat tcctagaatt
    58141 ttattgaaca aaaagttata aaatcacttt tattgtaact caaccttgtt ttacattaca
    58201 gaaattaaca atagcgatta aaattttatt ttccaacaat aaaaacgcac aaatattttt
    58261 attgaggcag ctgtatcgct tctcattgta aaaatatctg tgcatattat ctatttatct
    58321 caatctaacg tcttaactaa cttgttgtat cttgtaaaat cttatccata gaatgcgcaa
    58381 cactatcacg gtacatctca ccaaatttca ataaatggac catcaataaa tataaacgat
    58441 aaaattcaag tctataggat gcaccttttg cgagtggata atgtttatta tacgcatcat
    58501 aaaattcgct cgtaaaacca ccaaataccg ttgtaatacc gatatcgaat tctctgtcac
    58561 catataatgg cgctggatca aataacgccg gacgaccatc ttgtaagaac atataattac
    58621 caccccatag gtcaccatgt aataaagacg gtttactttg atgcttttct aattccgcca
    58681 caatttgacg tcgcactttg tcatatactt tgatatcgtt ggcatcccat agccctctat
    58741 ttagcaactc atctttcaaa tggtccaagc gtttgtcgac aaaaatggta caccaatcgt
    58801 cttgccaatg attatcaaaa gaaatatcgc caccttcata aggtaatgag aagccaaatt
    58861 tgccttcttc ttgttgctga ctgtgtaatt gagctacgag ttgccctaat tggcgttgac
    58921 tccctgaagc cccttcttct aaatacgtca tcactaaata cgcatcaccg ttaacctcgc
    58981 cacttgcaat tactctaggt gccgtgatac ctgcacgttc aaattcattt aaacctgcaa
    59041 tttctgcagc ataaaatgat tctttacgtc cacgttggac aagtaagaaa aatgtatccg
    59101 tatctgtttc gactcgatat gcttcgttta catcaccacc actcactggt gaaatctctt
    59161 taatatcttt taaaggtaaa tgctctaacc attgttcatt catacaaaca ccttccaatc
    59221 tacttacttt atactataat attgcctaat taggatgatt taatcttaaa aacgttattc
    59281 aaacttgcgt caattttcaa aactttttag ttttaactag gaaattatgt atggaaatgc
    59341 tagtctatta aaagaatata tttcacatat attaagttga ggatagtaag cctcaaatat
    59401 aatgaaatag gagcatactc atgtacaaat taattaaacc tttcttattc aaaatcgaac
    59461 ccgaaaaagc acacggacta actatcgatg cattaaaaac gttacaaaag tttccggttt
    59521 tattcccagt cgtcgataaa ctatttactt ataagaatcc aacgttatca caaacgatac
    59581 aaggtaatac gtacgacaat ccaattggct tagcagctgg tttcgacaaa tcttgcgaag
    59641 taccaaaagc attggaacac cttggattcg gtgctttaga attaggtggt atcacaccta
    59701 aacctcaacc gggtaaccct caaccacgca tgtttagatt attagaagat gacgccttga
    59761 taaatcgaat gggcttcaat aatattggta tgaacaaagc actaagtcat ttgcgtaaaa
    59821 atgcttatca agtacctgtt ggtatcaatg ttggtgtgaa taaaatgaca ccttatgaag
    59881 cgcgttatca agattatata aaggttattg atacgtttaa acacgacgtt tcatttttca
    59941 cagtcaacat cagttctcca aatactgaaa atcttcaaaa cttccatgat aaagatgaat
    60001 tttcaatgtt atgccaagct ttaacagcat ttaaaaaaca acatgatgta acagtgccaa
    60061 tttatttaaa actaacgtct gatatggatt tcgatggctt aaaagcacta ttaccagcga
    60121 ttactgagac atttgacggt atcatcttag caaacacaac gcgacaacga gatggtttaa
    60181 cttctgctaa taaagtcgaa gaaggcggtt tgagtggtcg tccattattt gaacgtaatt
    60241 taaaattgat taagtatgct tatcagcaaa caaatggtga atttttaatt ataggtacag
    60301 gcggcgtatt cagtactgaa gatgcaatca aaatgatgcg tcacggtgcg tcacttattc
    60361 aaatttattc atcacttgtt attgaaggcc caggtttaac taagaaaatg aacaaaggca
    60421 tcgcacgtta cttaaaagat catcattttg acaatgtcag tgatattata ggactagatg
    60481 cctaatccaa catagtcaaa taatacttta aaacttgctg aatcgtctta ttaaaattat
    60541 gatataactc aagaagatat atttatcacc atttttcaaa ataatggctt aaatatcaca
    60601 tatcattaaa tatagcgttt tataacggag gcgtttgtta tgactaaaga aacagataat
    60661 aaaaaaacag tagcgacttc tgacaacaaa gacacacagc aacaaaatca acctgaagaa
    60721 gatgaacgtc aaggtttgaa tggttatcgt aaaacagatc ttgatttaga aatcgagcaa
    60781 gagctacgtg aaatgatgaa aacaggagaa aatgaaacga aaaatgacta taaaaagttt
    60841 aaagtgtttt cacttatttc aacacttgtc attgtcattt tagcaattat aagatttgtt
    60901 cataaaatga tgtaattaga gtgagacatt gttttatgtc tcaggatcca gttattcatt
    60961 atatctacaa tatttacgat tatataaata acccgagatt ttagtatgat tcatttcact
    61021 aaaatctcgg gtttctattt gataattttt aatgggatat ggcatgtata cgttcttgcc
    61081 ttttatctca tttccaatga ttaatctgga tattgttcta aaaatgcttt cgcttcttta
    61141 ttaactgttt taaaatcaat accttgttgc atcgctgcaa agacacatcc acaataacac
    61201 tgcctaaaga tattatagtc attacacatt tctatggaac gctcataacc tttacttttc
    61261 ttaaaatcac ttggcaaata gttcacatcg tatatttttt ggacatccat accaagttca
    61321 ttgattaatt gtgcgttctt tttaggtgat aatgtgattg cactgccaaa ataatcatag
    61381 ccatgttcta cagctgcttt cgctacaata tccaaacgca tttcaaaaca agcggtacaa
    61441 cgtaagccgc cttctttttc atcagctaat tctttatcct tcaccatttt cacaaactta
    61501 tgcggttcat aaggtgcttc aatatacttc acattcgcac cagtcttgcg attaaaatct
    61561 tccacaaatt gttcttgcac tttagcacgt cgtaagtact catttttcgg atgaatattt
    61621 gaattcgcga aataaattgc aatgtctgca tattgtgtta aaaactctaa tgtatatgta
    61681 ctacaaggtg cacaacaact atgcaataag attttaggtc tgattgcttc tctttcccac
    61741 tgcccgatta atttcttcaa caccttgtca taattaattt tttgattttt cattttactg
    61801 ataataggtt cagcattaat catgatttaa cctcccaca
//
LOCUS       DAKTLH010000002        53781 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.002, whole genome shotgun sequence.
ACCESSION   DAKTLH010000002 DAKTLH010000000
VERSION     DAKTLH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 53781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53781
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.002"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(218..1708)
                     /locus_tag="O0394_000064"
     CDS             complement(218..1708)
                     /locus_tag="O0394_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HCY0498181.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDKALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
     gene            complement(1900..2121)
                     /locus_tag="O0394_000065"
     CDS             complement(1900..2121)
                     /locus_tag="O0394_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCY0498182.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            complement(2121..2621)
                     /locus_tag="O0394_000066"
     CDS             complement(2121..2621)
                     /locus_tag="O0394_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCY0498183.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            complement(2633..3061)
                     /gene="msrB"
                     /locus_tag="O0394_000067"
     CDS             complement(2633..3061)
                     /gene="msrB"
                     /locus_tag="O0394_000067"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCY0498184.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            complement(3054..3587)
                     /gene="msrA"
                     /locus_tag="O0394_000068"
     CDS             complement(3054..3587)
                     /gene="msrA"
                     /locus_tag="O0394_000068"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCY0498185.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            complement(3673..4512)
                     /gene="fakB2"
                     /locus_tag="O0394_000069"
     CDS             complement(3673..4512)
                     /gene="fakB2"
                     /locus_tag="O0394_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCY0498186.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            complement(4527..5006)
                     /locus_tag="O0394_000070"
     CDS             complement(4527..5006)
                     /locus_tag="O0394_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCY0498187.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            complement(5206..6162)
                     /locus_tag="O0394_000071"
     CDS             complement(5206..6162)
                     /locus_tag="O0394_000071"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCY0498188.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            complement(6586..7023)
                     /locus_tag="O0394_000072"
     CDS             complement(6586..7023)
                     /locus_tag="O0394_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCY0498189.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            complement(7039..8163)
                     /locus_tag="O0394_000073"
     CDS             complement(7039..8163)
                     /locus_tag="O0394_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HCY0498190.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIQEVMDGVLEEVLTTYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPVVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            complement(8201..8452)
                     /locus_tag="O0394_000074"
     CDS             complement(8201..8452)
                     /locus_tag="O0394_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HCY0498191.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            complement(8464..8658)
                     /locus_tag="O0394_000075"
     CDS             complement(8464..8658)
                     /locus_tag="O0394_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HCY0498192.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            8903..9607
                     /locus_tag="O0394_000076"
     CDS             8903..9607
                     /locus_tag="O0394_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HCY0498193.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            10148..10549
                     /locus_tag="O0394_000077"
     CDS             10148..10549
                     /locus_tag="O0394_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCY0498194.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVQNALLYTDSKLIADSIEAGYVKNANFKPYFDQIEIFEKDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKKNK"
     gene            complement(10608..41873)
                     /gene="ebh"
                     /locus_tag="O0394_000078"
     CDS             complement(10608..41873)
                     /gene="ebh"
                     /locus_tag="O0394_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HCY0498195.1"
                     /translation="MNYRDKIQKFSIRKYTVGTFSTVIATLVFLGFNTSQAHAAETNQ
                     PASVVKQKQQSNNEQTENRESQVQNSQNSQNGQSLSATHENEQPNISQANLVDQKVAQ
                     SSTTNDEQPASQNVNTKKDSATAATTQPDKEQSKHKQNESQSANKNGNDNRAAHVENH
                     EANVVTASDSSDNGNVQHDRNELQAFFDANYHDYRFIDRENADSGTFNYVKGIFDKIN
                     TLLGSNDPINNKDLQLAYKELEQAVALIRTMPQRQQTSRRSNRIQTRSVESRAAEPRS
                     VSDYQNANSSYYVENANDGSGYPVGTYINASSKGAPYNLPTTPWNTLKASDSKEIALM
                     TAKQTGDGYQWVIKFNKGHAPHQNMIFWFALPADQVPVGRTDFVTVNSDGTNVQWSHG
                     AGAGANKPLQQMWEYGVNDPHRSHDFKIRNRSGQVIYDWPTVHIYSLEDLSRASDYFS
                     EAGATPATKAFGRQNFEYINGQKPAESPGVPKVYTFIGQGDASYTISFKTQGPTVNKL
                     YYAAGGRALEYNQLFMYSQLYVESTQDHQQRLNGLRQVVNRTYRIGTTKRVEVSQGNV
                     QTKKVLESTNLNIDDFVDDPLSYVKTPSNKVLGFYSNNANTNAFRPGGAQQLNEYQLS
                     QLFTDQKLQEAARTRNPIRLMIGFDYPDAYGNSETLVPVNLTVLPEIQHNIKFFKNDD
                     TQNIAEKPFSKQAGHPVFYVYAGNQGNASVNLGGSVTSIQPLRINLTSNENFTDKDWQ
                     ITGIPRTLHIENSTNRPNNARERNIELVGNLLPGDYFGTIRFGRKEQLFEIRVKPHTP
                     TITTTAEQLRGTALQKVPVNISGIPLDPSALVYLVAPTNQTTNGGSEADQIPSGYTIL
                     ATGTPDGVHNTITIRPQDYVVFIPPVGKQIRAVVYYNKVVASNMSNAVTILPDDIPPT
                     INNPVGINAKYYRGDEVNFTMGVSDRHSGIKNTTITTLPNGWTSNLTKADKNNGSLSI
                     TGRVSMNQAFNSDITFKVSATDNVNNTTNDSQSKHVSIHVGKISEDAHPIVLGNTEKV
                     VVVNPTAVSNDEKQSIITAFMNKNQNIRGYLASTDPVTVDNNGNVTLHYRDGSSTTLD
                     ATNVMTYEPVVKPEYQTVNAAKTATVTIAKGQSFSIGDIKQYFTLSNGQPIPSGTFTN
                     ITSDRTIPTAQEVSQMNAGTQLYHITATNAYHKDSEDFYISLKIIDVKQPEGDQRVYR
                     TSTYDLTTDEISKVKQAFINANRDVITLAEGDISVTNTPNGANVSTITVNINKGRLTK
                     SFASNLANMNFLRWVNFPQDYTVTWTNAKIANRPTDGGLSWSDDHKSLIYRYDATLGT
                     QITTNDILTMLKATTTVPGLRNNITGNEKSQAEAGGRPNFRTTGYSQSNATTDGQRQF
                     TLNGQVIQVLDIINPSNGYGGQPVTNSNTRANHSNSTVVNVNEPAANGAGAFTIDHVV
                     KSNSTHNASDAVYKAQLYLTPYGPKQYVEHLNQNTGNTTDAINIYFVPSDLVNPTISV
                     GNYTNHQVFSGETFTNTITANDNFGVQSVTVPNTSQITGTVDNNHQHVSATAPNVTSA
                     TNKTINLLATDTSGNTATTSFNVTVKPLRDKYRVGTSSTAANPVRIANISNNATVSQA
                     DQTTIINSLTFTETVPNRSYARASANEITSKTVSNVSRTGNNANVTVTVTYQDGTTST
                     VTVPVKHVIPEIVAHSHYTVQGQDFPAGNGSSASDYFKLSNGSDIADATITWVSGQAP
                     NKDNTRIGEDITVTAHILIDGETTPITKTATYKVVRTVPKHVFETARGVLYPGVSDMY
                     DAKQYVKPVNNSWSTNAQHMNFQFVGTYGPNKDVVGISTRLIRVTYDNRQTEDLTILS
                     KVKPDPPRIDANSVTYKAGLTNQEIKVNNVLNNSSVKLFKADNTPLNVTNITHGSGFS
                     SVVTVSDALPNGGIKAKSSISMNNVTYTTQDEHGQVVTVTRNESVDSNDSATVTVTPQ
                     LQATTEGAVFIKGGDGFDFGHVERFIQNPPHGATVAWHDSPDTWKNTVGNTHKTAVVT
                     LPNGQGTRNVEVPVKVYPVANAKAPSRDVKGQNLTNGTDAMNYITFDPNTNTNGITAA
                     WANRQQPNNQQAGVQHLNVDVTYPGISAAKRVPVTVNVYQFEFPQTTYTTTVGGTLAS
                     GTQASGYAHMQNATGLPTDGFTYKWNRDTTGTNDANWSAMNKPNVAKVVNAKYDVIYN
                     GHTFATSLPAKFVVKDVQPAKPTVTETAAGAITIAPGANQTVNTHAGNVTTYADKLVI
                     KRNGNVVTTFTRRNNTSPWVKEASAATVAGIAGTNNGITVAAGTFNPADTIQVVATQG
                     SGETVSDEQRSDDFTVVAPQPNQATTKIWQNGHIDITPNNPSGHLINPTQAMDIAYTE
                     KVGNGAEHSKTINVVRGQNNQWTIANKPDYVTLDAQTGKVTFNANTIKPNSSITITPK
                     AGTGHSVSSNPSTLTAPAAHTVNTTEIVKDYGSNVTAAEINNAVQVANKRTATIKNGT
                     AMPTNLAGGSTTTIPVTVTYNDGSTEEVQESIFTKADKRELITAKNHLDDPVSTEGKK
                     PGTITQYNNAMHNAQQQINTAKTEAQQVINNERATPQQVSDALTKVRAAQTKIDQAKA
                     LLQNKEDNSQLVTSKNNLQSSVNQVPSTAGMTQQSIDNYNAKKREAETEITAAQRVID
                     NGDATAQQISDEKHRVDNALTALNQAKHDLTADTHALEQAVQQLNRTGTTTGKKPASI
                     TAYNNSIRALQSDLTSAKNSANAIIQKPIRTVQEVQSALTNVNRVNERLTQAINQLVP
                     LADNSALKTAKTKLDEEINKSVTTDGMTQSSIQAYENAKRAGQTESTNAQNVINNGDA
                     TDQQIAAEKTKVEEKYNSLKQAIAGLTPDLAPLQTAKTQLQNDIDQPTSTTGMTSASI
                     AAFNEKLSAARTKIQEIDRVLASHPDVATIRQNVTAANAAKSALDQARNGLTVDKAPL
                     ENAKNQLQHSIDTQTSTTGMTQDSINAYNAKLTAARNKIQQINQVLAGSPTVEQINTN
                     TSTANQAKSDLDHARQALTPDKAPLQTAKTQLEQSINQPTDTTGMTTASLNAYNQKLQ
                     AARQKLTEINQVLNGNPTVQNINDKVTEANQAKDQLNTARQGLTLDRQPALTTLHGAS
                     NLNQAQQNNFTQQINAAQNHAALETIKSNITALNTAMTKLKDSVADNNTIKSDQNYTD
                     ATPANKQAYDNAVNAAKGVIGETTNPTMDVNTVNQKAASVKSTKDALDGQQNLQRAKT
                     EATNAITHASDLNQAQKNALTQQVNSAQNVQAVNDIKQTTQSLNTAMTGLKRGVANHN
                     QVVQSDNYVNADTNKKNDYNNAYNHANDIINGNAQHPVITPSDVNNALSNVTSKEHAL
                     NGEAKLNAAKQEANTALGHLNNLNNAQRQNLQSQINGAHQIDAVNTIKQNATNLNSAM
                     GNLRQAVADKDQVKRTEDYADADTAKQNAYNSAVSSAETIINQTTNPTMSVDDVNRAT
                     SAVTSNKNALNGYEKLAQSKTDAARAIDALPHLNNAQKADVKSKINAASNIAGVNTVK
                     QQGTDLNTAMGNLQGAINDEQTTLNSQNYQDATPSKKTAYTNAVQAAKDILNKSNGQN
                     KTKDQVTEAMNQVNSAKNNLDGTRLLDQAKQTAKQQLNNMTHLTTAQKTNLTNQINSG
                     TTVAGVQTVQSNANTLDQAMNTLRQSIANKDATKASEDYVDANNDKQTAYNNAVAAAE
                     TIINANSNPEMNPSTITQKAEQVNSSKTALNGDENLAAAKQNAKTYLNTLTSITDAQK
                     NNLISQITSATRVSGVDTVKQNAQHLDQAMASLQNGINNESQVKSSEKYRDADTNKQQ
                     EYDNAITAAKAILNKSTGPNTAQNAVEAALQRVNNAKDALNGDAKLIAAQNAAKQHLG
                     TLTHITTAQRNDLTNQISQATNLAGVESVKQNANSLDGAMGNLQTAINDKSGTLASQN
                     FLDADEQKRNAYNQAVSAAETILNKQTGPNTAKTAVEQALNNVNNAKHALNGTQNLNN
                     AKQAAITAINGASDLNQKQKDALKAQANGAQRVSNAQDVQHNATELNTAMGTLKHAIA
                     DKTNTLASSKYVNADSTKQNAYTTKVTNAEHIISGTPTVVTTPSEVTAAANQVNSAKQ
                     ELNGDERLREAKQNANTAIDALTQLNTPQKAKLKEQVGQANRLEDVQTVQTNGQALNN
                     AMKGLRDSIANETTVKTSQNYTDASPNNQSTYNSAVSNAKGIINQTNNPTMDTSAITQ
                     ATTQVNNAKNGLNGAENLRNAQNTAKQNLNTLSHLTNNQKSAISSQIDRAGHVSEVTA
                     TKNAATELNTQMGNLEQAIHDQNTVKQSVKFTDADKAKRDAYTNAVSRAEAILNKTQG
                     ANTSKQDVEAAIQNVSSAKNALNGDQNVTNAKNAAKNALNNLTSINNAQKRDLTTKID
                     QATTVAGVEAVSNTSTQLNTAMANLQNGINDKTNTLASENYHDADSDKKTAYTQAVTN
                     AENILNKNSGSNLDKTAVENALSQVANAKGALNGNHNLEQAKSNANTTINGLQHLTTA
                     QKDKLKQQVQQAQNVAGVDTVKSSANTLNGAMGTLRNSIQDNTATKNGQNYLDATERN
                     KTNYNNAVDSANGVINATSNPNMDANAINQIATQVTSTKNALDGTHNLTQAKQTATNA
                     IDGATNLNKAQKDALKAQVTSAQRVANVTSIQQTANELNTAMGQLQHGIDDENATKQT
                     QKYRDAEQSKKTAYDQAVAAAKAILNKQTGSNSDKAAVDRALQQVTSTKDALNGDAKL
                     AEAKAAAKQNLGTLNHITNAQRTDLEGQINQATTVDGVNTVKTNANTLDGAMNSLQGS
                     INDKDATLRNQNYLDADESKRNAYTQAVTAAEGILNKQTGGNTSKADVDNALNAVTRA
                     KAALNGADNLRNAKTSATNTIDGLPNLTQLQKDNLKHQVEQAQNVAGVNGVKDKGNTL
                     NTAMGALRTSIQNDNTTKTSQNYLDASDSNKNNYNTAVNNANGVINATNNPNMDANAI
                     NGMANQVNTTKAALNGAQNLAQAKTNATNTINNAHDLNQKQKDALKTQVNNAQRVSDA
                     NNVQHTATELNSAMTALKAAIADKERTKASGNYVNADQEKRQAYDSKVTNAENIISGT
                     PNATLTVNDVNSAASQVNAAKTALNGDNNLRVAKEHANNTIDGLAQLNNAQKAKLKEQ
                     VQSATTLDGVQTVKNSSQTLNTAMKGLRDSIANEATIKAGQNYTDASPNNRNEYDSAV
                     TAAKAIINQTSNPTMEPNTITQVTSQVTTKEQALNGARNLAQAKTTAKNNLNNLTSIN
                     NAQKDALTRSIDGATTVAGVNQETAKATELNNAMHSLQNGINDETQTKQTQKYLDAEP
                     SKKSAYDQAVNAAKAILTKASGQNVDKAAVEQALQNVNSTKTALNGDAKLNEAKAAAK
                     QTLGTLTHINNAQRTALDNEITQATNVEGVNTVKAKAQQLDGAMGQLETSIRDKDTTL
                     QSQNYQDADDAKRTAYSQAVNAAATILNKTAGGNTPKADVERAMQAVTQANTALNGIQ
                     NLDRAKQAANTAITNASDLNTKQKEALKAQVTSAGRVSAANGVEHTATELNTAMTALK
                     RAIADKAETKASGNYVNADANKRQAYDEKVTAAENIVSGTPTPTLTPADVTNAATQVT
                     NAKTQLNGNHNLEVAKQNANTAIDGLTSLNGPQKAKLKEQVGQATTLPNVQTVRDNAQ
                     TLNTAMKGLRDSIANEATIKAGQNYTDASQNKQTDYNSAVTAAKAIIGQTTSPSMNAQ
                     EINQAKDQVTAKQQALNGQENLRTAQTNAKQHLNGLSDLTDAQKDAVKRQIEGATHVN
                     EVTQAQNNADALNTAMTNLKNGIQDQNTIKQGVNFTDADEAKRNAYTNAVTQAEQILN
                     KAQGPNTSKDGVETALENVQRAKNELNGNQNVANAKTTAKNALNNLTSINNAQKEALK
                     SQIEGATTVAGVNQVSTTASELNTAMSNLQNGINDEAATKAAQKYTDADREKQTAYND
                     AVTAAKTLLDKTAGSNDNKAAVEQALQRVNTAKTALNGDERLNEAKNTAKQQVATMSH
                     LTDAQKANLTSQIESGTTVAGVQGIQANAGTLDQAMNQLRQSIASKDATKSSEDYQDA
                     NADLQNAYNDAVTNAEGIISATNNPEMNPDTINQKASQVNSAKSALNGDEKLAAAKQT
                     AKSDIGRLTDLNNAQRTAANAEVDQAPNLAAVTAAKNKATSLNTAMGNLKHALAEKDN
                     TKRSVNYTDADQPKQQAYDTAVTQAEAITNANGSNANETQVQAALNQLNQAKNDLNGD
                     NKVAQAKESAKRALASYSNLNNAQSTAAISQIDNATTVAGVTAAQNTANELNTAMGQL
                     QNGINDQNTVKQQVNFTDADQGKKDAYTNAVTNAQGILDKAHGQNMTKAQVEAALNQV
                     TTAKNALNGDANVRQAKSDAKANLGTLTHLNNAQKQDLTSQIEGATTVNGVNGVKTKA
                     QDLDGAMQRLQSAIANKDQTKASENYIDADPTKKTAFDNAITQAESYLNKDHGANKDK
                     QAVEQAIQSVTSTENALNGDANLQRAKTEAIQAIDNLTHLNTPQKTALKQQVNAAQRV
                     SGVTDLKNSATSLNNAMDQLKQAIADHDTIVASGNYTNASPDKQGAYTDAYNAAKNIV
                     NGSPNVITNAADVTAATQRVNNAETGLNGDTNLATAKQQAKDALRQMTHLSDAQKQSI
                     TGQIDSATQVTGVQSVKDNATNLDNAMNQLRNSIANKDDVKASQPYVDADRDKQNAYN
                     TAVTNAENIINATSQPTLDPSAVTQAANQVSTNKTALNGAQNLANKKQETTANINQLS
                     HLNNAQKQDLNTQVTNAPNISTVNQVKTKAEQLDQAMERLINGIQDKDQVKQSVNFTD
                     ADPEKQTAYNNAVTAAENIINQANGTNANQSQVEAALSTVTTTKQALNGDRKVTDAKN
                     NANQTLSTLDNLNNAQKGAVTGNINQAHTVAEVTQAIQTAQELNTAMGNLKNSLNDKD
                     TTLGSQNFADADPEKKNAYNEAVHNAENILNKSTGTNVPKDQVEAAMNQVNATKAALN
                     GTQNLEKAKQHANTAIDGLSHLTNAQKEALKQLVQQSTTVAEAQGNEQKANNVDAAMD
                     KLRQSIADNATTKQNQNYTDASQNKKDAYNNAVTTAQGIIDQTTSPTLDPTVINQAAG
                     QVSTTKNALNGNENLEAAKQQASQSLGSLDNLNNAQKQTVTDQINGAHTVDEANQIKQ
                     NAQNLNTAMGNLKQAIADKDATKATVNFTDADQAKQQAYNTAVTNAENIISKANGGNA
                     TQAEVEQAIKQVNAAKQALNGNANVQHAKDEATALINSSNDLNQAQKDALKQQVQNAT
                     TVAGVNNVKQTAQELNNAMTQLKQGIADKEQTKADGNFVNADPDKQNAYNQAVAKAEA
                     LISATPDVVVTPSEITAALNKVTQAKNDLNGNTNLATAKQNVQHAIDQLPNLNQAQRD
                     EYSKQITQATLVPNVNAIQQAATTLNDAMTQLKQGIANKAQIKGSENYHDADTDKQTA
                     YDNAVTKAEELLKQTTNPTMDPNTIQQALTKVNDTNQALNGNQKLADAKQDAKTTLGT
                     LDHLNDAQKQALTTQVEQAPDIATVNNVKQNAQNLNNAMTNLNNALQDKTETLNSINF
                     TDADQAKKDAYTNAVSHAEGILSKANGSNASQTEVEQAMQRVNEAKQALNGNDNVQRA
                     KDAAKQVITNANDLNQAQKDALKQQVDAAQTVANVNTIKQTAQDLNQAMTQLKQGIAD
                     KDQTKANGNFVNADTDKQNAYNNAVAHAEQIISGTPNANVDPQQVAQALQQVNQAKGD
                     LNGNHNLQVAKDNANTAIDQLPNLNQPQKTALKDQVSHAELVTGVNAIKQNADALNNA
                     MGTLKQQIQANSQVPQSVDFTQADQDKQQAYNNAANQAQQIANGIPTPVLTPDTVTQA
                     VTTMNQAKDALNGDEKLAQAKQEALANLDTLRDLNQPQRDALRNQINQAQALATVEQT
                     KQNAQNVNTAMSNLKQGIANKDTVKASENYHDADADKQTAYTNAVSQAEGIINQTTNP
                     TLNPDEITRALTQVTDAKNGLNGEAKLATEKQNAKDAVSGMTHLNDAQKQALKGQIDQ
                     SPEIATVNQVKQTATSLDQAMDQLSQAINDKAQTLADGNYLNADPDKQNAYKQAVAKA
                     EALLNKQSGTNEVQAQVESITNEVNAAKQALNGNDNLANAKQQAKQQLANLTHLNDAQ
                     KQSFESQITQAPLVTDVTTINQKAQTLDHAMELLRNSVADNQTTLASEDYHDATAQRQ
                     NDYNQAVTAANNIINQTTSPTMNPDDVNGATTQVNNTKVALDGDENLAAAKQQANNRL
                     DQLDHLNNAQKQQLQSQITQSSDIAAVNGHKQTAESLNTAMGNLINAIADHQAVEQRG
                     NFINADTDKQTAYNTAVNEAAAMINKQTGQNANQTEVEQAITKVQTTLQALNGDHNLQ
                     VAKTNATQAIDALTSLNDPQKTALKDQVTAATLVTAVHQIEQNANTLNQAMHGLRQSI
                     QDNAATKANSKYINEDQPEQQNYDQAVQAANNIINEQTATLDNNAINQAATTVNTTKA
                     ALHGDVKLQNDKDHAKQTVSQLAHLNNAQKHMEDTLIDSETTRTAVKQDLTEAQALDQ
                     LMDALQQSIADKDATRASSAYVNAEPNKKQSYDEAVQNAESIIAGLNNPTINKGNVSS
                     ATQAVISSKNALDGVERLAQDKQTAGNSLNHLDQLTPAQQQALENQINNATTRGEVAQ
                     KLTEAQALNQAMEALRNSIQDQQQTEAGSKFINEDKPQKDAYQAAVQNAKDLINQTNN
                     PTLDKAQVEQLTQAVNQAKDNLHGDQKLADDKQHAVTDLNQLNGLNNPQRQALESQIN
                     NAATRGEVAQKLAEAKALDQAMQALRNSIQDQQQTESGSKFINEDKPQKDAYQAAVQN
                     AKDLINQTGNPTLDKSQVEQLTQAVTTAKDNLHGDQKLARDQQQAVTTVNALPNLNHA
                     QQQALTDAINAAPTRTEVAQHVQTATELDHAMETLKNKVDQVNTDKAQPNYTEASTDK
                     KEAVDQALQAAESITDPTNGSNANKDAVDQVLTKLQEKENELNGNERVAEAKTQAKQT
                     IDQLTHLNADQIATAKQNIDQATKLQPIAELVDQATQLNQSMDQLQQAVNEHANVEQT
                     VDYTQADSDKQNAYKQAIADAENVLKQNANKQQVDQALQNILNAKQALNGDERVALAK
                     TNGKHDIDQLNALNNAQQDGFKGRIDQSNDLNQIQQIVDEAKALNRAMDQLSQEITDN
                     EGRTKGSTNYVNADTQVKQVYDETVDKAKQALDKSTGQNLTAKQVIKLNDAVTAAKKA
                     LNGEERLNNRKAEALQRLDQLTHLNNAQRQLAIQQINNAETLNKASRAINRATKLDNA
                     MGAVQQYIDEQHLGVISSTNYINADDNLKANYDNAIANAAHELDKVQGNAIAKAEAEQ
                     LKQNIIDAQNALNGDQNLANAKDKANAFVNSLNGLNQQQQDLAHKAINNADTVSDVTD
                     IVNNQIDLNDAMETLKHLVDNEIPNAEQTVNYQNADDNAKTNFDDAKRLANTLLNSDN
                     TNVNDINGAIQAVNDAIHNLNGDQRLQDAKDKAIQSINQALANKLKEIEASNATDQDK
                     LIAKNKAEELANSIINNINKATSNQAVSQVQTAGNHAIEQVHANEIPKAKIDANKDVD
                     KQVQALIDEIDRNPNLTDKEKQALKDRINQILQQGHNGINNAMTKEEIEQAKAQLAQA
                     LQDIKDLVKAKEDAKQDVDKQVQALIDEIDQNPNLTDKEKQALKYRINQILQQGHNDI
                     NNALTKEEIEQAKAQLAQALQDIKDLVKAKEDAKNAIKALANAKRDQINSNPDLTPEQ
                     KAKALKEIDEAEKRALQNVENAQTIDQLNRGLNLGLDDIRNTHVWEVDEQPAVNEIFE
                     ATPEQILVNGELIVHRDDIITEQDILAHINLIDQLSAEVIDTPSTATISDSLTAKVEV
                     TLLDGSKVIVNVPVKVVEKELSVVKQQAIESIENAAQQKINEINNSVTLTLEQKEAAI
                     AEVNKLKQQAIDHVNNAPDVHSVEEIQQQEQAHIEQFNPEQFTIEQAKSNAIKSIEDA
                     IQHMIDEIKARTDLTDKEKQEAIAKLNQLKEQAIQAIQRAQSIDEISEQLEQFKAQMK
                     AANPTAKELAKRKQEAISRIKDFSNEKINSIRNSEIGTADEKQAAMNQINEIVLETIR
                     DINNAHTLQQVEAALNNGIARISAVQIVTSDRAKQSSSTGNESNSHLTIGYGTANHPF
                     NSSTIGHKKKLDEDDDIDPLHMRHFSNNFGNVIKNAIGVVGISGLLASFWFFIAKRRR
                     KEDEEEELEIRDNNKDSIKETLDDTKHLPLLFAKRRRKEDEEDVTVEEKDSLNNGESL
                     DKVKHTPFFLPKRRRKEDEEDVEVTNENTDEKVLKDNEHSPLLFAKRRKDKEEDVETT
                     TSIESKDEDVPLLLAKKKNQKDNQSKDKKSASKNTSKKVAAKKKKKKAKKNKK"
     gene            complement(42271..43662)
                     /gene="norB"
                     /locus_tag="O0394_000079"
     CDS             complement(42271..43662)
                     /gene="norB"
                     /locus_tag="O0394_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HCY0498196.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEIFYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
     gene            complement(43819..45141)
                     /locus_tag="O0394_000080"
     CDS             complement(43819..45141)
                     /locus_tag="O0394_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0498197.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            complement(45172..46212)
                     /gene="tdcB"
                     /locus_tag="O0394_000081"
     CDS             complement(45172..46212)
                     /gene="tdcB"
                     /locus_tag="O0394_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCY0498198.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            complement(46307..47425)
                     /gene="ald"
                     /locus_tag="O0394_000082"
     CDS             complement(46307..47425)
                     /gene="ald"
                     /locus_tag="O0394_000082"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCY0498199.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE"
     gene            complement(47900..48778)
                     /locus_tag="O0394_000083"
     CDS             complement(47900..48778)
                     /locus_tag="O0394_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HCY0498200.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
     gene            complement(48798..52238)
                     /locus_tag="O0394_000084"
     CDS             complement(48798..52238)
                     /locus_tag="O0394_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCY0498201.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEGLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLIGRQETTYQVATDNAQDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDMSYSTIEDFINSDLEKLKSHLNKNQ
                     LAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQALKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTDYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTRVYLDQIHQRLFLEQSLITERIKKYFNKQLTEQIAPIVQQLADLHVIINPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLELLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            52542..>53781
                     /locus_tag="O0394_000085"
     CDS             52542..>53781
                     /locus_tag="O0394_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCY0498202.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWMIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGAL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     EQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVAIGMGFVSWIKRTKN
                     TAIKVPHRVKKPASISLIICL"
BASE COUNT        14301 a         9580 c         8914 g        20986 t
ORIGIN      
        1 actatttaaa aagtatatgc tcattgcata ttaaaccatg aattgttatc agcgatgata
       61 gaaattgcgt tatttacatc atttgtaaat gtctaattta tatcttttag agttagacgt
      121 caataaaaat aaacacgcct cctaaattgc tatctttgca aatttaggag gcgtgtcatt
      181 aacataataa taatacaatt ttagtagtgt gtatcgctta ttttaaaata ttaatcaact
      241 tatcgagaac atcatcatgt ttattagctt tttcaactaa taactcagta aatttattat
      301 tcgtttcttt attaaattca ccagttacct caagtttatt cgcttgttgg aaagctttaa
      361 cttgattttc taaagcttta tcaaattgcg ttgattcatt atcaacttta taacctaaag
      421 ctgataaacc aattttaata gttttaatat ttttatcatc gtctccaact ttaaatgttt
      481 tcgtattagg aatgacattt aaagattgat atttaggtgt gtcaatagta acgtctggtt
      541 taatgccttt accgtgaata taatgaccat ctggcgttaa ccatttcatt tcagtatatt
      601 ttaacaatga accatcctta aactctcttg tagtttgtac gacacctttg ccgaatgttt
      661 ttgacccata aactttagct ttattatagt cttttagcgc accagtaaac acttcagaag
      721 cgctagctga accttcattc actaagatgg atatatccat gtctttcgct tcttttaacg
      781 catcattaga agtttgaatt gcttcagtat ctttaccttt ttctagttta acaacagttt
      841 ttcctttatc gataaaaata tttgccattt taacagcttc atctagtagt ccacctggat
      901 tatttcttaa atctaaaaca atctttttca aaccatcttt gtgagctttt agaactgcat
      961 ctttcaattc acctgatgta tcattctgga atttattaat agtaataact ccaactttac
     1021 cttttttctt atactcaaca cttttaacat gaattttttc acgtttaatc ttaacgtctt
     1081 tttcttcact acctcgttga acagttaaag tgacttcagt gttttcttta ccacgaacat
     1141 ctttgacaac ttcatctaat gctttacctt taattgattt tccatttact ttagtaatga
     1201 catctttagg acgaatgcca gcacgttctg ctggagatcc cttcatagga ctagtaacca
     1261 taatttgatc atttttcttt tgcatttctg caccaatacc tacaaaatca cctgaaacac
     1321 cttcattaaa ggatttcgtt tgttcttttg ttaaatattc agaataagga tcttttaatt
     1381 ctttgaccat gccatcaatt gcagctttac ttaacttgtc agagtcctgt tttttgtaat
     1441 aatcactatt taagatttta tacacatttt caattttatt taagtttgct tgatcagttt
     1501 tgtttaaacc acttattttt tgatttataa aaatatatgc aacaactgtg atgacagctg
     1561 ttattaggat tgtaccaatt aatattgata tgaattgcca acgttttaag tgaacgcgtt
     1621 tcgatgaatt agtttcttgg tcttgattgc ttgttgcaat ttcagcgcgt tcatcatcgg
     1681 atgaagatgt gtgttgctta tcatccattg tatcacttcc ttattatgta ttatgtactt
     1741 ttaaattctg tgtagttaat catatcaatt tatgcaagat agcgcaccat atttcactat
     1801 aattgattaa tttttagtca caacttagtt tattttaaat taaaaccagg ataacgaatt
     1861 gcatcaccct ggttctaact attattatta aaaattatat taaaatttta gccattccgt
     1921 atattcttca tataaatcat caaatacaga cattggcaat gtgaaatcac catgtgtctc
     1981 aatataatca gacagtatgt taaaatcatc atcgtgtttt gggaaagcaa gatcgtcaaa
     2041 tatctcttct gctagacgac ctttatcgtc gtgtcgacca cgaactgtca tgacaaattg
     2101 ataaaacgaa tagtttttca ttagttcatt gtcacatcaa tcacttttgt ttcaccttta
     2161 atcacagcat tttcatcata aatattaatt gaagctgctt gatcagtgtt agtaattata
     2221 attggtgaaa ttacagattt agcgttatta ttaatatatt caaggttgaa tcttactaat
     2281 ggatctccga cgttaacttc gtcaccacta gacactaaca cttcaaatcc ttcaccgtct
     2341 aattgaactg tgtctaaacc gatatgaact aataattcta atccgttatc tgcttttaac
     2401 ccaatcgcat gcttagttgg aaagacattg tcaacacgtc ctgcaattgg agacacaact
     2461 tctccttcag ttggattaat accaaaacct tcgcccatca ttttttgtgc gaatacagga
     2521 tctggaatat cttcaatttt cacgaattct ccagttaatg gtgcataaat tgcgatatct
     2581 ttctgaactt ctttgccttt tccgaataat tttttaaaca tactttccac tcctacttat
     2641 caaaatgtga tattaaatcg ccataaccca attcttctaa cttttcatat ggaataaatt
     2701 gaattgcagc ggaattgata cagtatctta agccgccact ttctttaggt ccatcattaa
     2761 agacatgtcc taaatgacta tttgattctt ctgaacgcac ttcagttctc aacataccaa
     2821 atgatttgtc gactaattct ataatttcat cgtcatcaag cgctttggaa aagctaggcc
     2881 atccacattc agaatgaaac ttttcttcag atgtaaataa aggtttacca gaaattttat
     2941 ctacataaat tcctttagca aaatgattcc aatattcatt cataaatggt ggttcagtgc
     3001 cgttttcttg tgtaacaata tattctatat ctgttagttc acttttatct tttttaagca
     3061 ttttgattcc cccaatgtga ttctataaac gcttttctac ctgaaccacg ttgatattgg
     3121 taataatgta ccgggttctt tttgtaataa tcttgatggt agtcttcagc tggatagaaa
     3181 tttttatatg gtttaatagg tgtaatcact ggtttcttga aaataccttg ttcatttaat
     3241 tgttgctttt taaactcagc agcctttttc tgatgttcat catgatagaa aatgactggt
     3301 tgatagcttt cgcctctatc gaaaaattgc ccttgatcat cagttgggtc aaatgttttg
     3361 aaatatatgt ctaatatatt ttcaaaggaa gtaacctctg gatcaaacgt aatttgtact
     3421 gcttcgacat ggccggtttg attcgtacat acctgttcat aagttgggtt gtcaacatga
     3481 ccgccactat aaccagatac gactgacttg atgcctggat atgatgtaaa tggtttaacc
     3541 atgcaccaga aacatcctcc tgctaatgtt gcatattctt ttgtcatatg aattacctcc
     3601 tctatctatc taattataaa tttagtctat aaaggattga aatggaagta attaacactg
     3661 aaaagattaa atttacttct taaggactac gaggccaatc gcaccttgac cagtatgtgc
     3721 agaaataact ggtgtagtta catttatatc gtaattattc acatgaaaag cttcattaaa
     3781 aactttcttc aatttatcaa catattcaat gacgttagca tgtgcgacac caatggattt
     3841 gatttcatga tctcctataa attcagcaat ttcctttttc aagtattgga tactagaatt
     3901 ttgagttctc gcattgtgca caagctctaa gcgaccatca tctagtgtac caattggttt
     3961 aattttcata agattaccaa tcaaaccttt tgttttacta attctgccac ctttaattaa
     4021 ttgattcaat tgccctataa ctacaaataa tttaatgttt tctcttaaat gatttaactt
     4081 tttaactatt tcagaagttg agacaccttc ttttacaagc tctactaggt gttgtatttg
     4141 ataccctaaa ccaaaagaaa tagattttga atcaataaca gttacattag catctaccat
     4201 ttgacttgct tggtaagcag tgttatatgt accacttaat cctgaagaaa gatgaatact
     4261 tatgatttca gagccatctt ttcctagttc ttcataagca gatataaatt cacctatggc
     4321 tggctgactt gtctttacat cttcatcatt ttcaatatga ttaataaatt cttctgatgt
     4381 aatatctact tggtcaacgt atgaagctcc ttcaatagtt aaacttaaag gaattacatg
     4441 aatgttgttt gcttctaagt attctttaga taaatcggat gttgagtctg ttactataat
     4501 ctgttttgtc atggtcgttt tcccccttat tttttacgaa ttaaatgtag aaaggtatgt
     4561 ggaattgtat ttttctcatc tagtttacct tcaactgaag aggcaacttc ccagtcttca
     4621 aatgtataag gtggaaagaa cgtatcacca cggaatttac cttcaataac agtaatatac
     4681 atgtcgtcca ctttatcaat catttcttca aataatgttt gccctccaaa tatgaaaaca
     4741 tggcccggta gttggtaaat atcttcaata gagtgaatta catcaacgcc ctctacgttg
     4801 aaacttgtat ctgaagtaag tacaacattt cgacgattcg gtagtggttt accaatcgat
     4861 tcaaatgtct tacgacccat tactaaagta tgacctgttg ataatttttt aacatgcttc
     4921 aaatcatttg gtaggtgcca aggtaattga ttttcaaaac caattactcg ttgcaagtca
     4981 tgtgcaacta gaatggataa agtcataatt atcctccttc ttctatcatt tcatttttta
     5041 ttactaagtt atctttaatt taacacaatt tttatcataa agtgtgatag aaataatgat
     5101 tttgcataat ttatgaaaac gtttaacaca aaaaagtact tttttgcact tgaaaatact
     5161 atgatgtcat tttgatgtct atatggttag ctaactatgc aatgactaca ctgctattgg
     5221 agcttttatt gctggatgtg attcatagtc aacaatttcc aaatcttcat aatttatgtc
     5281 gaaaatagac ttgtcactgt taatttttaa tgttggagga ttgaagcttt cacgtgctaa
     5341 ttgtgtttga atcgcatcaa tatgatttga ataaatatgt gcatctccaa atgtatgcac
     5401 aaattcaccc acttcaagtc cacattcttt ggcaataagg tgtgtcaata aagcgtagct
     5461 tgcgatatta aatggcacac ctaaaaagat atctgcgcta cgttggtata actggcaact
     5521 taacttacca tcttggacat aaaactggaa catggtatga caaggcggaa gtgccattgt
     5581 atcaatttct gttggattcc atgcagatac gatgtgtcgc cttgaatctg gattatgctt
     5641 aatttgttca attactgttt taagttgatc aaaatgatta ccatctttat caacccaatc
     5701 tcgccattgt ttaccataaa catttcctaa atccccgaat tgcttcgcaa atgtatcatc
     5761 ttcaagaata cgttgcttaa attgtttcat ttgttcttta tattgttcgt taaattcagg
     5821 atcactcaat gcacgatgcc cgaaatctgt catatctgga cctttatact cgtctgattt
     5881 gatataattt tcaaaagccc attcgttcca tatattatta ttatatttta ataagtattg
     5941 gatgtttgta tctcctttaa tgaaccataa taattcggtt gctactaatt taaaagaaac
     6001 tttctttgtc gttaatagtg gaaatccttt agataagtca aagcgaagtt gatgaccaaa
     6061 tttcgaaatc gtacctgtat ttgtgcgatc atttcgtgta tttcctattt ctaaaacttc
     6121 ttcacaaaga ctgtgatatg ctgcatcaaa tgaattcaac atatgcgata acacctcatt
     6181 ttcattattt atagtatgta tatttagttt gatataactt aactttatgt agcattttgt
     6241 tatcactcat tttaggaata tgatattaat atcatgaatt ccgttacttt atttataaaa
     6301 tgctgattaa gtacctaccc catcgtaacg tgatatatgt ttccaattgg taattgttta
     6361 cccaaatcta taactttaat gctaaaaaat tttaaaaaag aggttaacac atgatttgaa
     6421 tattatgttt gatgtcctat taaaacagtt aaatttctag aaaatatagt tggtaaaaac
     6481 ggactttatt taacaaatag aatacaacta tattctctat tttcaatgac agacaccatt
     6541 tttaatatta taaaatgtgt taacctttat atttatttat gtgtactatt tacaattttc
     6601 gtcaaaggca tcctttaagt ccattgcaat gtcattaata tctctacctt cgataaattc
     6661 tctaggcata aaataaacta aatcttgacc tttgaataaa gcatacgaag gactagatgg
     6721 tgcttgctga atgaattctc gcattgtagc agttgcttct ttatcttgcc cagcaaaaac
     6781 tgtaactgta tttgtaggtc tatgttcatt ttgtgttgca actgctactg cagctggtct
     6841 tgctaatcca gctgcacagc cgcatgtaga gttaataact acaaaagtag tgtcatcagc
     6901 atttacttgg ttcatatact ccgatactgc ttcgctcgtt tctaaacttg taaaaccatt
     6961 ttgagttaat tcgccacgca tttgttgcgc aatttctttc atataagcat catatgcatt
     7021 catatttaat tcctccaatt aaattgttct gtttgccatt tgtttccata ctgaaccaag
     7081 tgcttcatct ccgttttcaa tacgagatat ggccatttca atttgtaatt taacttcaaa
     7141 cgcattgtca ttaatatggg cttttagtgc gggaagctgc tctgcattac cttcatcaaa
     7201 gataaacata gcagcacgcc acctaacgat tttctgtgga tcatctaata gtagcaccat
     7261 ttctggtagt gcctctggat accctaaatc gcttatgcaa tcccctgctg ttcttcttac
     7321 aacaggactt ttatcacgaa gccccttata taaatacggt aaaatttctt tactttcaat
     7381 catacctaat aatacaatcg cttgacgtct aaccggtact ttttcatcag ataaagctaa
     7441 atcaagcagc ggtatatctt caaaagtcgg ctttggaaaa tggtttaaca ttcgtaatcg
     7501 agtcttccaa ttatcagttg catgatattc atccaaagag acatgtcgat agaaatgata
     7561 attattagtt gcgtgatttt cttctaaagc atgttttacc aatacgggta actgtgattc
     7621 tggataggta gttagcactt cttctaggac accatccatt acttcttgaa tatttccata
     7681 gcgatttcct aaatctagcc atttacgcat aaaaacaata ttgtcatgtg ctgtttgcgc
     7741 ttgagtcata tggtcaacat atgtttgtgg taattgttct cttaattctt ggtcagcaga
     7801 agttagctta atttgatacg gtataccctt aaaagttaat aattcagctt taatttcacc
     7861 aaaatgattg tcaatttgag gttcatttac agattctaaa accttattcg catcagaaaa
     7921 agcagcttta atatcaggta atatgacttc ccaatcagct tttggtgcct tatcaacagc
     7981 taagaagttc atgacatgaa aaatggaagt gataccatct atagataaca actgatttat
     8041 aaatcttggt tgtgtttctt ctactttttt ataagtatta gataacttgt cttctcttgt
     8101 atatgacaaa acaactttca ttgtatttgg acttggtgtt ggctctatac gtaaaatttc
     8161 catatacttt attccccccg aatactagtt gatgaaaaat ttattcgaat acagcctcta
     8221 cttttggcaa tactgtttcc caatttgcgt cattttcttt atctactgaa ataaagtcca
     8281 taacatggaa aattgattta acgccttcaa cctttaagat gtcattaata aatgctggct
     8341 gtgaatcatc aactttagta tacgtatctg atgtcatacc ttctctgctt tcactaagtg
     8401 taatcttcat tgtgttgtgg ttcggtgttt ctgatataga tataattttc atttgacttc
     8461 ctcctattga agttcatttc ctaattgttt tatttctttc cctattgaac actcattgat
     8521 acaaaaatga tgcgccttcg ttttcccctc taatttacgc agacgagttt tgattggaca
     8581 ttgattgcaa taagtattca ttaaatcatc tattttcgcg agtgcatttt gctctgaaaa
     8641 agttaaaata cgcgacactt cctttattac ttcaaaatat tgcaaccttg taaataagtg
     8701 ttactgtttt aaatttgaca taaaaatatt ataacatttt tgaccctaag atttgaatga
     8761 aaccttttac aattctatac gtaaaatgta tttatattca aatgctattg catcgcaaac
     8821 gatttttcag taaaatgagt tagttattta ttgagcggtt tctaaacacc cgcatgatca
     8881 aaatttagga ggacatttta atatgtataa tgaaatttta ggactagtta cgtttattgc
     8941 aacgttcgtg cttatggtac ttatgtatcg cttttttggt aaacaaggtt taattgcatg
     9001 ggttgcaatt ggcacaatca ttgccaacat acaagtgatt aaaactgtag aaatctttgg
     9061 tatttcagca actttaggta atgtcatgtt tgcttctatt tatttagcaa cagatatatt
     9121 aaacgatatt tatgggcgta gagttgcaaa aagagcagtt tggttaggct tttcatcaac
     9181 attaattatg attattgtta tgcaattgtc attacatttt attcctgcac cagaagatat
     9241 ggcacaaaaa gcattacacg caatctttga tgttgtgcca cgcattgctt taggctcaat
     9301 cgtcgcatat attattggtc aacatattga tgtatttatc ttttcactaa ttaaaaaagt
     9361 atttagttct gataaaacgt ttttcatccg tgcatatggt agtacatttt taagctcaat
     9421 aattgataca gctttatttg tagctatcgc atttatcgga agtttacctg gtacagttgt
     9481 atttgaaata tttattacaa cgtacgtatt aaaattagct tcaacagttt tcaatgtacc
     9541 atttggatat attgctaaat cattttatcg taaaggtaag attgataagt tagatcaagg
     9601 ctattaacgc acttactaca caaaagaata agttttaagg gagtgggaca gaaataatat
     9661 tttcgcaaaa tttatttcgt cgtcccaccc caacttgcat tgtctgtaga aattgggaat
     9721 ccaatttctc tttgttgggg cccatcccaa acttgcacat tattgtaagc tgacttttcg
     9781 tcatcttctg tgttgggacc cacaccccaa ctcgcattgc ctgtagaatt tcttttcgaa
     9841 attctctgtg ttggggcccc tgactagaat tgaaaaaagc ttgttacaag cgcattttcg
     9901 ttcagtcaac tactgccaat ataacatcgt agagcataga acattgattt atgtcccagc
     9961 ctgaagatga tttttttagg aagtatatta cagtactacg tattagaaaa tcatctttga
    10021 atcagcgagt attttaaaca cgtgttactt atttaagtga tgcgtgtttt ttaatggatt
    10081 acccaacata ttttgtatta cagtaatagt aactttaact aagtattgaa tagaaagtag
    10141 gtttattatg gcgaaaataa attttgatgc tgcgacgaaa ggaaatccag gcataagtac
    10201 atgtgccatt gtaatcaaag aagatgagca gcattataca tatacacatg agttaggcga
    10261 aatggataac cacactgcag aatgggctgc atgtatttat gcactagaac atgcacgtga
    10321 attaaatgtt caaaacgcac tattatatac agactcaaag ctaattgcag atagcattga
    10381 agctggttat gtgaaaaacg caaatttcaa accttatttt gatcaaatag aaatatttga
    10441 aaaagatttt gatttattat ttgttaaatg gataccgaga gaacaaaaca aagaagcgaa
    10501 tcaacacgct caacaggcat tgtataaatt aattaaaaag aataaataaa aggagcaccg
    10561 actgatgtga tgtcaatcgg tgctcctcta tttatcaaag aaacaaatta ttttttattt
    10621 ttcttagctt tctttttctt ctttttagct gctacctttt tagaagtatt ttttgatgct
    10681 gactttttgt ctttggattg gttatctttt tgatttttct ttttagccaa taataaagga
    10741 acgtcctcat ctttagattc aatactagtt gttgtttcaa catcttcctc tttatctttg
    10801 cgtcgttttg cgaataagag tggtgaatgt tcgttatctt tcaacacttt ttcatctgtg
    10861 ttttcatttg taacttccac atcttcttca tcttctttac gacgacgttt tggtaagaag
    10921 aacggcgtat gtttaacttt atcgagtgac tcgccattat ttagcgaatc tttttcttca
    10981 acagtaacat cttcttcatc ttcttttctg cgacgtttcg caaataaaag tggtaaatgt
    11041 tttgtatcgt ctaaagtctc ttttattgaa tctttattat tatctcttat ttctaattct
    11101 tcctcttcat cttctttacg acgacgtttg gcaatgaaga accagaaact agctagtaaa
    11161 ccagagatac ccaccacacc aatagcgttt ttaataacat taccgaaatt attactaaag
    11221 tgacgcatat gaagtggatc aatgtcatca tcttcatcaa gtttcttttt atgtccaata
    11281 gtcgaactgt taaatggatg atttgcagtt ccataaccaa ttgttaaatg gctattagat
    11341 tcatttccag tacttgacga ttgtttagca cgatcagatg ttacaatttg tactgctgaa
    11401 attcgagcaa taccattgtt caatgcagcc tcaacttgct gtaatgtatg cgcattatta
    11461 atatctctaa ttgtttcaag cacaatttcg ttaatttgat tcattgctgc ttgtttttca
    11521 tcagctgtgc caatttcact atttcgaata ctatttattt tttcatttga aaagtcttta
    11581 attctactaa tagcttcttg cttgcgttta gctagttctt ttgctgttgg attagctgct
    11641 ttcatttgag ctttaaattg ttccaattgc tcacttattt catcgatgct ttgcgcacgt
    11701 tgaatcgctt gaattgcttg ttcttttaat tgatttaact tagcaatagc ttcttgcttc
    11761 tctttatctg ttagatcagt acgagctttg atttcatcaa tcatatgttg aattgcatct
    11821 tcaatcgatt taattgcatt tgattttgct tgttcaatcg taaattgttc tggattaaat
    11881 tgttcaatat gcgcttgttc ttgttgttga atttcttcaa ctgaatgaac atcaggtgca
    11941 ttgttaacat gatcaattgc ttgttgttta agcttattaa cttctgcaat tgcagcttct
    12001 ttttgttcca gtgttaatgt cacactatta ttgatttcat taatcttttg ttgtgccgca
    12061 ttttcgattg attcaattgc ctgttgtttg actactgaca attctttttc tacaactttt
    12121 acaggaacat taacaatcac ttttgatcca tcaagcaatg taacttcaac ttttgctgtt
    12181 aagctatcag aaatcgttgc agttgatggt gtatcgatga cttctgctga aagctgatca
    12241 attaagttta tgtgtgcaag aatatcttgt tctgtaatga tgtcatcacg atgtacaatg
    12301 agttcaccat taactaggat ttgctcaggt gttgcttcaa aaatttcatt tacagcaggt
    12361 tgttcatcaa cctcccatac atgtgtattt ctaatgtcat ctaaacctaa gtttaatcct
    12421 cgatttaatt gatctatagt ttgagcattc tcaacgtttt gtagtgctcg tttttcagct
    12481 tcgtcaattt ctttgagcgc ttttgctttt tgctcaggtg ttaaatctgg atttgaattg
    12541 atttgatcac gcttcgcatt agctaaggct tttattgcat ttttcgcatc ttctttagct
    12601 ttcactaaat ctttgatgtc ttgcaatgct tgtgcaagtt gtgcttttgc ctgctcaatt
    12661 tcttctttag tcagcgcatt gttaatgtcg ttatgacctt gttgaagtat ttgattaatt
    12721 cgatatttaa gtgcttgttt ttccttatct gttagatttg gattttgatc gatttcgtca
    12781 attaaagctt gaacttgttt atcaacatct tgtttcgcat cttctttagc tttcactaaa
    12841 tctttgatgt cttgtaatgc ttgcgcaagt tgtgctttgg cttgttcaat ttcttcttta
    12901 gtcatcgcat tgttaatgcc gttatgacct tgttgaagta tttgattaat acgatcttta
    12961 agtgcttgtt tttccttatc tgttagattt ggatttcgat caatttcgtc aattaatgct
    13021 tgaacttgct tatcaacgtc tttattggca tcaatttttg cttttggtat ttcattggca
    13081 tgcacttgtt caatcgcgtg gttgcctgct gtttgaactt gagatacagc ctgattactt
    13141 gttgctttat taatgttgtt gatgatgctg tttgccaatt cttctgcttt atttttcgca
    13201 ataagcttgt cttgatccgt cgcatttgaa gcttcgattt cttttagctt attagctaaa
    13261 gcttgattaa tagattgaat tgccttgtct ttagcatctt gtagtcgttg atcaccatta
    13321 agattatgga ttgcatcatt gactgcttgg attgcgccat tgatatcatt cacatttgtg
    13381 ttatcactat ttagcaatgt atttgctaga cgtttggcat catcgaagtt tgttttagca
    13441 ttatcgtcag cgttttggta attgacagtt tgctctgcat ttggaatttc attgtcaact
    13501 aaatgtttca atgtttccat tgcatcattt aagtcaattt gattattaac aatatctgtt
    13561 acatctgata cagtatcggc attgttaatt gctttatgtg caagatcttg ttgctgttga
    13621 tttaatccat ttaacgaatt aacaaacgca tttgctttat ctttggcatt tgcaaggttt
    13681 tggtctccat ttaatgcatt ttgagcatcg ataatatttt gtttcaattg ctctgcttca
    13741 gcttttgcaa ttgcattacc ttgcacttta tctaactcat gtgctgcatt cgcaattgca
    13801 ttatcataat ttgctttcaa attgtcatct gcattgatgt aatttgtgct gctgataaca
    13861 ccaaggtgct gttcgtcaat atattgttgt actgcaccca ttgcattatc taatttagtt
    13921 gctctattaa ttgctcgaga tgctttattt agcgtttcag cattattaat ttgttggatt
    13981 gctaattgtc tttgagcatt gtttagatgt gttaattgat ccaatctttg taatgcttca
    14041 gctttacgat tattaagtct ttcttcacca tttaatgctt tcttagctgc agtgactgca
    14101 tcatttaatt tgataacttg ttttgcagtt aagttttgac cagtcgattt atcaagtgct
    14161 tgtttcgctt tatcaaccgt ttcatcatat acttgtttga cttgtgtatc tgcattgaca
    14221 tagttcgtgc tacctttcgt gcgtccttca ttgtcagtga tttcttgtga caattgatcc
    14281 attgcacgat taagtgcctt agcctcatct acaatttgtt ggatttgatt taaatcgttt
    14341 gattgatcga tgcgaccttt aaatccatct tgttgagcat tgtttaatgc attcaattgg
    14401 tcgatgtcat gtttaccatt tgttttagca agtgctacac gttcatcacc atttaatgct
    14461 tgttttgcat ttaaaatatt ttgaagtgct tgatccactt gttgcttatt cgcattttgt
    14521 ttcaatacat tttcagcatc agcaatagct tgtttataag cattttgttt atctgaatct
    14581 gcttgtgtgt aatctacagt ttgctcaacg ttagcatgtt cattaactgc ttgttgtaat
    14641 tgatccatag attgattcaa ttgcgttgct tgatctacta attcagcaat tggttgaagt
    14701 ttcgtcgctt gatcaatgtt ttgtttagca gttgcaattt gatcagcatt taaatgtgtt
    14761 aattggtcaa tagtttgttt cgcttgtgtt ttagcttcag cgactctctc attaccattt
    14821 aactcatttt ctttttcttg aagcttagtt aatacttggt ctacagcgtc tttattcgca
    14881 tttgaaccat tagttggatc tgtaatgctt tctgcagctt gtaacgcttg atctactgct
    14941 tcttttttat cagttgacgc ttcagtgtaa tttggttgag ccttatctgt attcacttga
    15001 tcaactttat ttttcaatgt ttccatcgcg tgatcaagtt cagtagcagt ttgaacatgt
    15061 tgtgcaacct ctgttcttgt aggcgctgca tttatagcat cagttaatgc ttgttgttgt
    15121 gcatgattta agtttggcaa tgcatttaca gttgttactg cttgttgttg atcacgagca
    15181 agtttttgat caccatgtag attatctttt gcagttgtta ctgcttgtgt caattgttct
    15241 acttgtgatt tgtcgagtgt tggattacct gtttggttaa ttaaatcttt tgcattttga
    15301 actgctgctt ggtaagcatc tttttgcggt ttatcttcat tgataaactt gctaccagat
    15361 tctgtttgtt gttgatcttg aatactatta cgtaatgctt gcattgcttg atcaagcgct
    15421 tttgcttcag ctaatttttg tgctacttcg ccacgagttg ctgcgttgtt tatttggctt
    15481 tcaagtgctt gacgttgcgg attattcaaa ccatttaatt gatttaaatc agtaaccgca
    15541 tgttgtttat cgtctgcaag tttttgatca ccgtgtaggt tatctttagc ttggttaaca
    15601 gcttgtgtca attgttcaac ttgtgcttta tcaagcgttg gattgttagt ttgattaatt
    15661 aaatcttttg cattttgaac tgctgcttgg taagcatctt tttgtggttt atcttcattg
    15721 ataaacttgc tacccgcttc cgtttgctgt tgatcttgaa tgctattacg taaagcttcc
    15781 attgcttggt taagtgcttg tgcttcagtt aatttttgtg ctacttcgcc acgagttgtt
    15841 gcattattaa tttgattttc tagcgcttgt tgttgagctg gtgttaattg atctaaatga
    15901 tttagagaat ttccagcagt ttgcttatct tgagctaatc gttcaacacc atctaatgca
    15961 tttttagatg atattactgc ttgagtcgca cttgatacat tacctttatt gatagttgga
    16021 ttatttaatc ctgcaatgat agactcagca ttttgaactg cttcatcata ggattgtttt
    16081 ttattcggtt ctgcattgac atatgcactg ctcgcacgtg ttgcatcttt gtcagcaata
    16141 ctttgttgta atgcatccat aagttgatct aatgcttgtg cttcagtcaa atcttgctta
    16201 actgctgttc tagttgtttc actatcaatt aacgtatctt ccatatgttt ttgtgcattg
    16261 tttagatgtg ctaattgact aaccgtttgc ttagcatgat ctttatcatt ttgtaacttc
    16321 acatcaccat gtaatgctgc tttcgttgta ttcacagttg tcgctgcttg attaatcgca
    16381 ttattatcta atgttgcagt ttgttcattg ataatattat ttgcggcttg aacagcttga
    16441 tcatagtttt gttgctctgg ttgatcttcg ttgatatatt tgctatttgc tttagttgct
    16501 gcgttatctt gaatgctctg tcttaaacca tgcattgctt ggttaagcgt attcgcattt
    16561 tgttcaattt gatgaactgc agttactaaa gttgcagctg taacttggtc ttttaatgct
    16621 gttttttgag gatcatttaa gcttgttaaa gcatcaattg cttgcgtcgc atttgtttta
    16681 gcaacttgta aattatggtc tccatttaac gcttgaagtg ttgtttgaac tttagtaata
    16741 gcttgttcta cttctgtttg gttcgcattt tgaccagttt gtttgttaat cattgctgct
    16801 gcttcattta ccgctgtatt ataagcagtt tgtttatcag tatcagcatt gatgaagtta
    16861 ccacgttgtt caacggcttg atgatctgca atcgcattaa ttaagttacc catcgcagta
    16921 tttaaagatt ctgctgtttg tttgtgacca ttaactgcag caatatcaga tgattgcgta
    16981 atttgtgatt gtaactgttg cttttgcgca ttattcaaat gatctaattg atcaagtctg
    17041 ttgtttgctt gttgtttagc tgctgcaagg ttttcatcac catctaatgc aactttcgta
    17101 ttattcactt gtgtcgttgc accattaaca tcatctggat tcatcgtagg cgatgtagtt
    17161 tgattaatga tattattagc agctgttaca gcttggttat agtcattttg tctttgcgca
    17221 gttgcatcat gataatcttc agacgctaat gtcgtttgat tatccgcaac actatttctt
    17281 aataattcca tcgcatgatc taacgtttgt gctttttgat taatcgtagt gacatctgta
    17341 acaagtggcg cttgtgtaat ttgactttca aatgattgtt tttgtgcatc atttaagtgt
    17401 gttaagttcg ccaattgttg ttttgcttgt tgttttgcat ttgccaaatt gtcattacca
    17461 tttaatgctt gtttcgctgc gttcacttca ttagtgatgc tttcaacttg tgcttgtact
    17521 tcattagtac cactttgttt attcaataat gcttcagctt ttgctactgc ctgtttatac
    17581 gcattttgtt tgtcaggatc tgcatttaag taattaccgt ccgctaatgt ttgagcttta
    17641 tcattaatag cttgtgataa ttgatccatt gcttgatcta ggctcgttgc tgtttgttta
    17701 acttggttca ctgtagcaat ttcaggcgat tgatcgattt gaccttttaa tgcttgtttt
    17761 tgagcatcgt ttaaatgcgt catcccactt acggcatctt tagcattttg cttttcagtt
    17821 gccaatttag cttcaccgtt taagccattt ttagcatcag tcacttgagt taatgcacgt
    17881 gttatttcat ctgggttaag cgttggattt gtcgtttgat tgataatacc ttccgcttga
    17941 gacactgcat ttgtatatgc tgtttgctta tcggcatcag catcatgata gttctcactt
    18001 gctttgacag tatctttgtt tgcaatacct tgtttcaagt tactcattgc tgtattcaca
    18061 ttttgtgcat tttgtttagt ttgttcaact gtagctaacg cttgtgcttg attgatttgg
    18121 ttacgtaatg catcacgttg tggttgattt aaatcgcgta acgtatcaag atttgctaaa
    18181 gcttcttgtt tcgcttgtgc taatttttca tcaccgttta atgcatcttt cgcttgattc
    18241 atagttgtca ctgcttgtgt tactgtatca ggcgtcaata caggtgttgg tatgccattt
    18301 gcgatttgtt gcgcttggtt agccgcattg ttatatgctt gttgtttgtc ttgatccgct
    18361 tgtgtaaagt caactgactg tggtacttga ctgttcgctt gaatttgttg tttcaatgta
    18421 cccattgcat tatttaacgc atcagcattt tgcttaatag cattaacacc tgtaacaagt
    18481 tctgcatgcg acacttggtc ttttaatgct gttttttgtg gttgatttaa gtttggtaac
    18541 tgatcaatgg ctgtatttgc attgtcttta gcaacttgta agttatggtt accgtttaaa
    18601 tcacccttag cttgattcac ttgttgtaac gcttgagcca cttgttgtgg atccacgttt
    18661 gcatttggtg taccactaat tatttgttca gcatgtgcta ccgcattgtt gtaagcattt
    18721 tgcttatcag tatcagcatt gacaaagtta ccatttgctt tagtttggtc tttatctgca
    18781 ataccttgtt tcaattgtgt cattgcttga tttaaatctt gtgctgtttg cttaatcgtg
    18841 tttacatttg caacagtttg cgcagcatcg acttgttgtt ttaatgcatc tttttgcgct
    18901 tgatttaaat catttgcatt tgtaatcact tgtttcgctg catcttttgc acgttgtaca
    18961 ttgtcattac cattcaatgc ttgtttcgct tcgttcacac gttgcatcgc ttgttccact
    19021 tcagtttgac ttgcattgct gccatttgct ttagataaaa taccttctgc atgtgaaacc
    19081 gcattagtat aagcatcttt cttagcttga tctgcatcag taaagttaat gctatttaat
    19141 gtctcagttt tatcttgtaa tgcattgttt aagttagtca tagcattatt cagattttga
    19201 gcattttgct taacattatt aactgttgca atatctggtg cttgttcaac ttgagttgtt
    19261 agcgcttgtt tttgagcatc atttaaatga tctagtgtac caagtgttgt cttagcatct
    19321 tgtttggcat cagctaattt ttgattaccg ttaagtgctt gatttgtgtc attcacttta
    19381 gttaatgctt gttgaattgt atttggatcc attgttggat ttgttgtttg ttttaacaat
    19441 tcttctgctt ttgttactgc attatcatat gctgtttgct tgtcagtatc agcatcgtga
    19501 tagttctcgc tacctttaat ttgtgcttta ttcgcaatac cttgtttcaa ttgtgtcatc
    19561 gcgtcattaa gcgttgtcgc cgcttgttga atagcattga cgtttggtac aagtgttgct
    19621 tgcgtgattt gtttgctgta ttcatcacgt tgcgcttggt ttaagtttgg caattgatca
    19681 atagcatgtt gaacattttg tttcgccgtt gctaagtttg tattaccatt taaatcattt
    19741 ttagcttgcg taactttatt taacgctgca gtaatttcgc taggtgtaac gacaacatca
    19801 ggcgtagcac taattaatgc ttcagctttc gctactgctt gattatatgc attttgctta
    19861 tcaggatctg cattgacaaa gttaccatca gcttttgttt gttctttatc tgcaatgcct
    19921 tgttttaatt gtgtcatagc attgtttaac tcttgtgctg tttgtttaac attgtttaca
    19981 ccagctacag tagttgcatt ttgaacttgt tgttttaatg cgtctttttg tgcttggtta
    20041 aggtcattag agctattaat taatgctgtt gcttcgtctt ttgcatgttg aacgttggca
    20101 ttaccattta atgcttgttt tgcagcatta acttgtttga ttgcttgttc aacttcagct
    20161 tgtgttgcat tgccgccatt agcttttgaa atgatatttt cagcatttgt aacagcagtg
    20221 ttatatgctt gttgttttgc ttgatctgca tcagtgaagt taactgtcgc tttcgtagca
    20281 tctttgtcag ctatcgcttg tttcaagtta cccatcgctg tatttaagtt ttgcgcattt
    20341 tgcttaattt gatttgcttc atcaacagta tgcgcgccat taatttgatc agtaactgtt
    20401 tgtttttgcg cattatttaa gttatctaat gaacctaatg attgtgacgc ttgttgtttc
    20461 gctgcctcta ggttttcatt accatttaat gcatttttag ttgtgcttac ttgtccagca
    20521 gcttgattga taacagtcgg atctaaagtt ggacttgtag tttgatcaat aataccttgt
    20581 gcagttgtga cagcattatt gtacgcatcc tttttattct gacttgcatc agtataattt
    20641 tggttttgtt ttgttgtcgc attatctgca atactttgac gtaatttgtc cattgctgca
    20701 tcaacattgt ttgctttttg ctcattacct tgtgcttctg caacagtagt cgattgttgt
    20761 accaattgtt ttaatgcctc tttttgtgca tttgttaaat ggcttaaacc gtcaattgct
    20821 gtatttgcgt gttgtttagc tttttcaagg ttttgagtac cattaagcgc tgcttttgta
    20881 gcattcactt gattcatagc tgcttcaact tgatctttag gcacgttcgt acctgtagat
    20941 ttatttaaaa tattttcagc attatgaacc gcttcattgt atgcattttt cttctctgga
    21001 tctgcatctg caaagttttg actgccaagt gtagtgtctt tatcattcaa gctatttttc
    21061 aagttaccca tcgctgtatt cagttcctga gcggtttgaa tggcttgcgt tacttcagct
    21121 acagtgtgcg cttgattgat gtttccagta acagcacctt tttgtgcatt gtttaagtta
    21181 tctaacgtag ataatgtttg gtttgcattg tttttagcat ctgttacttt tctatcacca
    21241 ttcaacgctt gtttcgtagt tgttacagtt gaaagtgctg cttcaacttg tgattggttc
    21301 gcatttgtac catttgcttg attaataata ttttcagcag cagttaccgc attgttgtat
    21361 gctgtttgtt tttctggatc tgcatctgta aagttaacac tttgtttcac ttgatctttg
    21421 tcttggattc cgttgattaa acgttccatt gcttgatcta attgttcagc tttagttttc
    21481 acttgattta ctgtgctaat atttggtgca tttgtcactt gcgtatttaa atcttgcttt
    21541 tgagcattat ttaaatgact taattggttg atgttagcag tcgtttcttg ctttttattc
    21601 gctaagtttt gtgcaccatt aagcgcagtt ttgttagtgc tcacttgatt agctgcttgt
    21661 gttactgcag atggatcaag tgtcggctga ctcgttgcat taatgatatt ttcagcattt
    21721 gtaactgctg tattgtatgc attttgttta tctctatctg catcaacata tggttgactc
    21781 gcttttacat catctttatt cgcaatacta tttcgaagtt gattcattgc attatcaaga
    21841 tttgtcgcgt tgtctttcac actttgaacg ccagttactt gtgtcgcgct atcaatttga
    21901 ccagtaatac tttgtttttg tgcatcagat aaatgtgtca tttgacgtaa tgcatcttta
    21961 gcttgttgct tcgcagttgc taagtttgta tcaccgttta aacctgtttc agcattatta
    22021 acacgttgtg ttgctgctgt aacatctgct gcatttgtaa tcacattagg cgaaccattt
    22081 acaatgtttt tcgcagcatt atatgcatca gtataagcac cttgcttatc aggactcgcg
    22141 ttagtgtaat taccactagc tacaattgtg tcatgatcag caattgcttg ttttaattga
    22201 tccatcgcat tattaagtga tgtagcacta tttttcagat cagttacacc tgatacacgt
    22261 tgcgcagcgt tcacttgttg ttttaatgct gttttttgtg gtgtattcaa atgtgtcaag
    22321 ttatcgatag cttgtatagc ttcagtttta gcgcgttgta agttcgcgtc accgttcaaa
    22381 gcattttcag tagacgttac actttgaatt gcttgttcaa cagcttgctt atctttattc
    22441 gcaccgtgat ctttatttaa gtaagattca gcttgtgtga tagcattatc aaatgctgtt
    22501 ttcttagttg gatctgcgtc gatgtaattt tcgctcgctt tagtttgatc tttatttgct
    22561 atagctgatt gtaatcgttg cattgcacca tctaagtctt gtgctttcgt tttaacacca
    22621 tttacaccgt tgactgttgt tgcgccttcg atttgtgatg ttaaatcttg tttttgtgca
    22681 ttatttaagt gtgttaatgt acctaagttt gctttcgcat ctgattttgc ttgtcttaca
    22741 tttgcgtcac cgtttaaagc attcttagca gtcgttactt gatttaatgc agcttcaact
    22801 tgtgctttcg tcatattttg accgtgtgct ttatctaaaa taccttgagc attcgtaaca
    22861 gcatttgtgt aagcatcttt cttaccttgg tcagcatctg taaagttcac ttgttgttta
    22921 acagtgtttt ggtcatttat accattttga agttgaccca ttgctgtatt taattcatta
    22981 gcagtatttt gtgcagcagt tacgcctgct actgtcgttg cattgtcaat ttgactaatt
    23041 gctgcagttg attgcgcatt attcaagtta ctatatgaag ctaatgcacg tttcgctgac
    23101 tcttttgctt gagcaacttt attatcacca ttcaagtcat ttttagcttg attcaattgg
    23161 tttagtgctg cttgaacttg tgtttcattc gcgttgctgc cattagcatt agtaattgct
    23221 tctgcttgtg taaccgcagt atcatacgct tgttgttttg gttgatccgc atctgtgtaa
    23281 ttgacactac gtttcgtatt atccttttca gcaagtgcat gtttcaaatt acccatcgct
    23341 gtgtttaacg atgttgcttt atttttagcc gctgtgacag ctgcaagatt tggtgcttga
    23401 tccacttcag catttgccgc agttcgttgt gcattgttca agtctgtcaa acgaccgata
    23461 tctgatttcg cagtttgttt tgctgctgct aatttttcat caccgttcaa tgcagacttc
    23521 gcactgttca cttggctcgc tttttggtta attgtatcag gattcatttc agggttattc
    23581 gttgcactaa taataccttc agcattagtt accgcatcat tgtatgcatt ttgtaaatct
    23641 gcattcgcgt cttgataatc ttcgcttgat ttagtcgcat ctttagaagc aatactttgt
    23701 cttaattgat tcattgcttg atctaaagta ccggcattag cttgaatacc ttgaacacct
    23761 gcaacagtcg taccactttc gatttgcgat gttaagtttg ctttttgagc atcagttaag
    23821 tgtgacattg tcgctacttg ttgtttagct gtgttcttcg cttcatttaa tcgctcgtca
    23881 ccatttaatg ctgttttagc agtattcaca cgttgtaatg cttgttcaac agctgcttta
    23941 ttgtcatttg aaccagctgt tttatctaat aacgttttag ctgctgttac agcgtcattg
    24001 tatgcagttt gtttttctct atctgcatca gtatatttct gagctgcttt tgtagctgct
    24061 tcatcattaa taccattttg taagttgctc attgctgtat ttaattcaga tgccgttgta
    24121 gacacttgat ttacacctgc aactgttgtc gcaccttcaa tttgtgattt caatgcttct
    24181 ttttgtgcat tattaattga tgttaggtta ttcaatgcat ttttcgcagt tgtcttagcg
    24241 ttcgcaacat tttgattacc gttcaattcg tttttagcac gttgtacatt ttctaacgca
    24301 gtttcgacac cgtcttttga agtatttgga ccttgtgctt tatttaaaat ttgttcagct
    24361 tgcgtcactg catttgtata tgcattacgt ttcgcttcgt cggcatcagt gaagttaaca
    24421 ccttgcttaa tcgtattctg atcttgaata ccatttttca agttcgtcat agctgtattt
    24481 aatgcatccg cattattttg tgcttgtgtt acttcattaa catgcgttgc accttcgatt
    24541 tgacgtttca ctgcatcttt ttgagcgtca gttaagtcac ttaagccgtt caaatgttgc
    24601 ttcgcatttg tttgcgcagt tcttaagttt tcttgaccgt ttaacgcttg ttgtttagct
    24661 gtcacttggt ctttcgcttg attaatttct tgcgcattca ttgatggact agttgtttga
    24721 ccaatgattg cttttgctgc agtgactgca ctgttgtagt cagtttgttt gttttgactt
    24781 gcatctgtgt agttttgacc tgctttaatc gttgcttcat tcgcaatgct atctcgtaga
    24841 cctttcattg cagtgtttaa tgtttgtgca ttatcacgaa cagtttgaac atttggcaac
    24901 gtcgtcgctt gacccacttg ttctttaagt tttgcttttt gcggaccatt taaagaagtt
    24961 aaaccatcaa ttgcagtgtt agcattttgt ttcgctactt ctaaattatg attaccgttt
    25021 aactgcgtct tagcattcgt tacttgcgtt gctgcatttg taacatctgc tggtgttaac
    25081 gttggtgttg gtgtaccact aacgatattt tcggcagctg taactttttc atcatatgct
    25141 tgacgtttat tcgcatcagc attgacatag ttaccactag cttttgtctc agctttatca
    25201 gcaatggcac gctttaaagc tgtcatcgca gtatttaatt cagtcgcagt atgttcaaca
    25261 ccatttgctg cagatacacg tcctgcactt gttacttgtg cttttaatgc ttctttttgt
    25321 tttgtattta agtccgaagc atttgtaatc gctgtgttag cagcctgttt cgcacgatct
    25381 aagttttgaa taccgtttaa tgcagtattt gcttgtgtaa cagcttgcat tgctctttca
    25441 acatctgctt taggtgtatt accgccagct gttttattta aaatagttgc tgctgcattt
    25501 actgcttgag aataagcagt tcgtttagca tcatcagcat cttgataatt ttgactttgt
    25561 aacgtcgtgt ctttatcacg aattgatgtt tctaattgac ccatagcacc atctaattgt
    25621 tgcgctttgg ctttaactgt attaacacct tcaacatttg ttgcttgtgt aatttcattg
    25681 tctaacgctg tacgttgtgc attattaatg tgtgttaatg tacctaacgt ttgtttcgca
    25741 gctgctttag cttcatttaa tttcgcatca ccgttcaacg ccgtcttcgt actgttcaca
    25801 ttttgcaatg cttgttcaac tgctgctttg tctacatttt gaccactagc ttttgttaaa
    25861 attgctttcg ctgcatttac tgcttgatca taagctgatt tcttacttgg ctctgcatct
    25921 aggtatttct gagtttgttt tgtttgtgtc tcatcattga taccattttg taaactatgc
    25981 attgcgttat ttaattctgt tgcttttgca gtttcttgat ttacaccagc tactgttgtt
    26041 gcaccatcaa tgctacgcgt taacgcatct ttttgtgcat tgttaattga tgttaagtta
    26101 ttcaagttgt ttttcgcagt tgtcttagct tgagctaagt ttcgcgcacc atttaatgcc
    26161 tgttctttag ttgtcacttg tgatgttact tgcgtaatag tatttggttc catcgttggg
    26221 ttcgatgttt gattaatgat tgcttttgct gcagtaactg cactgtcgta ctcgttacga
    26281 ttatttggac ttgcgtcagt gtagttttga cctgctttaa ttgttgcttc attcgcaata
    26341 ctatctctta agcctttcat cgctgtattc aacgtttgag aactattttt aacagtttga
    26401 acaccatcta atgtagttgc actttgaact tgttctttta attttgcttt ttgtgcatta
    26461 ttcaattgtg ctaagccgtc aattgtattg ttggcatgct ctttcgctac acgtaagttg
    26521 ttatcaccat ttaatgctgt tttagccgca ttgacttgtg atgccgcact atttacgtca
    26581 ttgactgtta atgtcgcatt cggtgtacca ctaatgatat tttcagcgtt agtcactttt
    26641 gaatcatacg cttgacgttt ttcttgatca gcattgacat aattaccgct tgcttttgtt
    26701 ctttctttat cagcaatagc tgctttaagt gctgtcatcg cactgttcaa ttcagttgca
    26761 gtgtgttgaa cgttatttgc atcagataca cgttgtgcat tgttaacttg tgtttttaat
    26821 gcatcttttt gtttttggtt taagtcatgt gcgttgttaa ttgtgttcgt cgcatttgtt
    26881 ttagcttgag ctaagttttg tgcaccattt aacgctgctt ttgttgtatt gacttgattt
    26941 gccatgccat taatcgcatt agcatccata tttggattgt tcgttgcatt aataacacca
    27001 tttgcattat ttacagcagt attgtaatta tttttgttgc tgtcagatgc atcaagataa
    27061 ttttgacttg ttttcgtcgt attatcattt tggatacttg tacgtaatgc acccatggca
    27121 gtatttaacg tattaccttt atctttaaca ccatttacac ctgctacatt ttgcgcttgt
    27181 tcaacttgat gcttcaagtt gtctttttgt aattgtgtta agttaggtaa accatcaatc
    27241 gtatttgttg ctgaagtttt cgcatttctt aagttgtcag caccatttaa agccgctttc
    27301 gctcttgtaa ctgcatttaa tgcattatca acgtctgctt tagatgtgtt accaccagtt
    27361 tgtttattta aaatgccttc agccgctgtg acagcttgcg tatatgcatt tcgttttgat
    27421 tcatccgcat caagataatt ttgatttctt aatgtcgcat ctttatcatt gattgaacct
    27481 tgtaagctat tcattgcgcc gtctaatgta ttggcatttg tttttacagt attaacgcca
    27541 tcaacagtcg tcgcttgatt gatttggcct tctaagtcag tacgttgtgc attcgtaata
    27601 tggtttaaag tgcctaagtt ttgtttagct gccgctttcg cttctgccag ttttgcatca
    27661 ccattcaatg catctttcgt acttgttact tgttgtaatg cacggtcaac tgctgcttta
    27721 tctgaatttg aacctgtttg tttatttaaa attgctttcg cagcagctac agcttgatca
    27781 taagcagttt tcttactttg ttcagcgtca cgatattttt gagtttgttt tgttgcattt
    27841 tcatcatcaa taccatgttg taattgaccc atagctgtat taagttcatt tgcagtttgt
    27901 tggatacttg ttacatttgc aacacgttgc gcacttgtaa cttgtgcttt taacgcatct
    27961 ttttgcgctt tatttaagtt agtagcacca tcgatggcat ttgttgctgt ttgtttcgct
    28021 tgcgttaaat tatgtgtacc atctaatgca tttttcgttg atgtcacttg tgtagcgatt
    28081 tggttaattg cattagcatc catatttgga ttgcttgttg cattaatgac accattagca
    28141 ctatcaacag cattgttata gtttgttttg ttacgttctg tagcatcaag atagttttgg
    28201 ccatttttcg tagctgtgtt atcttgtatg ctatttctta acgtacccat agcaccattt
    28261 aatgtgttgg cacttgattt aacagtatct acacctgcaa cattttgtgc ttgttgcact
    28321 tgttgtttca atttatcttt ttgagcagtt gttaaatgtt gaagtccgtt tatagtagtg
    28381 tttgcatttg atttagcttg ctctaaatta tggttaccat ttagggcacc tttcgcatta
    28441 gcaacttgtg acaacgcgtt ttcaacggca gttttgtcta aatttgatcc actattttta
    28501 tttaaaatat tttctgcgtt cgtaacggct tgagtataag cagttttctt atctgaatca
    28561 gcatcatgat agttttcact tgctagtgta tttgttttat cattaatacc attttgcaag
    28621 ttagccatcg ctgtattcaa ttgtgtactc gtattagata cagcttcaac accagctaca
    28681 gttgttgctt gatcaatttt agttgttaag tcacgttttt gtgcattatt aattgacgtt
    28741 aagttattta atgcattttt agctgcattc ttcgcatttg taacgttttg atcaccattc
    28801 aatgcatttt tagcacttga aacattttga atagccgctt caacatcttg tttagacgta
    28861 tttgcacctt gcgttttatt cagaattgct tcagctctgc ttaccgcatt tgtatacgca
    28921 tcacgtttag ctttatctgc atcagtaaat ttaacacttt gtttaactgt gttttgatca
    28981 tggatagctt gttccaagtt acccatttgc gtattcaact cagttgctgc atttttagta
    29041 gcagttacct cactcacatg acctgcacga tcaatttgtg atgagatggc agatttttgg
    29101 ttatttgtta agtgtgataa tgtatttaag ttttgcttag cagtgttttg tgcatttctt
    29161 aagttttcag caccgtttaa accattttta gcattattca cttgtgttgt agcttgggta
    29221 atcgcactag tatccatagt cggattgtta gtttgattaa tgataccttt cgcatttgac
    29281 acagcgctat tatatgttga ttggttattc ggacttgcgt ctgtatagtt ttgacttgtt
    29341 ttgactgttg tttcgttagc aatactatct cttaagcctt tcattgcatt gttcaatgct
    29401 tgtccatttg tttgaacagt ttgtacgtct tctaatctat tggcttgtcc cacttgttct
    29461 tttaatttag ctttttgagg tgtatttaat tgtgttaatg catcaatagc agtattggcg
    29521 ttttgttttg cttcacgtaa tctttcgtca ccatttaatt cttgtttcgc gctgtttact
    29581 tgattagctg cagctgttac ttctgaaggt gtcgtaacaa ccgttggcgt accgctaata
    29641 atatgttcag cattggtaac tttagttgtg taagcatttt gtttagtgct atcggcatta
    29701 acatatttac tgcttgctaa cgtattcgtc ttatctgcga tggcatgttt taatgtgccc
    29761 attgccgtgt tcagttcagt cgcattgtgc tgtacatctt gtgcattaga tacgcgttga
    29821 gcaccattag cttgtgcttt taatgcatct ttttgttttt gatttaaatc agatgcgcca
    29881 ttgattgctg taatcgctgc ttgtttcgca ttgtttaagt tttgcgtacc atttaatgca
    29941 tgtttcgcat tattaacatt attaagtgct tgttcgactg ctgttttcgc tgtattcggt
    30001 ccagtttgtt tatttaaaat ggtttcggct gctgatacag cttgattgta tgcattacgt
    30061 ttttgctcat cagcatccaa gaagttttgg ctcgctaatg ttcctgactt atcgttgata
    30121 gccgtttgta agttacccat agcaccatct aaactattcg cattttgttt aacagattca
    30181 acaccagcta agtttgtagc ttgtgaaatt tgatttgtta aatcattacg ttgagctgta
    30241 gtgatatgcg ttaaagtacc taaatgttgt ttcgctgcgt tttgagctgc aattaatttt
    30301 gcatcaccat tcaatgcatc tttcgcatta ttaacacgtt gtaatgctgc ttcaactgca
    30361 ttttgcgcag tgtttggacc tgtcgattta tttaaaatcg ctttcgctgc agtaatagca
    30421 ttatcatact cttgttgttt atttgtatca gcatcacgat atttctcaga tgatttcact
    30481 tgagattcgt tgttaatacc attctgtaag ctagccatag cttggtctag atgttgcgca
    30541 ttttgtttta cagtatcaac accactcact cttgtcgcac tagtaatttg actaatcaaa
    30601 ttgttctttt gagcatctgt aatacttgtc aatgtgttta agtacgtttt cgcattttgt
    30661 tttgcagcag ctaagttttc atcaccgtta agtgccgttt tagaactatt cacttgctct
    30721 gctttttgtg taatcgtact tggattcatt tctggattac tattagcatt aataatcgtt
    30781 tcagcagcag ctactgcgtt gttatatgct gtttgcttat cattattagc atctacgtaa
    30841 tcttcacttg ctttagtcgc atctttgttg gcaatacttt gtcttaacgt attcatggct
    30901 tgatctaatg tattggcatt tgattgaacc gtttgaacac cagcgacagt agtaccacta
    30961 ttaatttggt ttgttaaatt cgttttttgt gcagttgtta aatgcgtcat attatttaac
    31021 tgctgttttg ctgtttgctt cgcttgatct aataaacgcg taccatctaa gttattttta
    31081 gcagaattca cttgattcat cgcttcagta acttgatctt tcgttttatt ttgaccattt
    31141 gatttattta aaatatcttt cgcagcttgt accgcatttg tgtatgctgt tttcttacta
    31201 ggtgtcgcat cttgatagtt ttgactatta agcgtcgttt gttcatcatt gattgcacct
    31261 tgcaagttac ccatcgctgt atttaaatct gtaccttgtt gtttaacagt atttacgcca
    31321 gcaatatttg atgcagcatt aattttagat ttaacatctg ctttttgtgc attatttaaa
    31381 tgtggtaatg catcaattgc tcttgcagca tctgttttag attgtgctaa tttttcataa
    31441 ccatttaatg catttttatt agaagtaaca gctgaagttg cacgattaac atcatcaaca
    31501 gacatcgttg gatttgttgt ttgattaatg attgtttcgg cacttgaaac tgcactgtta
    31561 tatgcatttt gtttagctgt atctgcatcc gcataatctt ctgtacgttt cacttgatct
    31621 ttatctgcaa cagcttgtct taagttaccc attgcactat tcaagtttgt tgcattttgc
    31681 ttaattgtat taactgcatc aatttgatgc gcaccattaa tttgcgattg taagttttga
    31741 cgttgtgcat tatttaaatt gtttaaatga cctaatgcag tattcgcttc ttgtttcgca
    31801 gcatttaact tagcttcacc attcaatgca tgttctttac ttgtgacatt tgataaagca
    31861 ttgttaacat cacttggtgt tataactgga tgttgtgcat taccattaat aatgtcattc
    31921 gcatggttgt atgcattgtt gtaatcattt ttcttattag tatctgcgtt gacataatta
    31981 tcactttgta cgacttggtt atgattagca acgccacgtt ttaaacctgt catagcagta
    32041 tttaagcttt gagtcgtttg tttaatatca tttactgctt gcacgttttg tgcactattc
    32101 acttgttgtg ttaatgcatt cttttgtgct tggtttaaat cacttgcatg cgtaatcgca
    32161 tttgttgctt ctgttttcgc acgttgtaag ttttgttgac catctaaagc atctttcgtc
    32221 gatttaacag atgctgcttt ttggttcact gtgttaacat ccatcgttgg attagtcgtt
    32281 tctccaatga cacctttagc cgcattaact gcattatcat acgcttgttt attagctggt
    32341 gttgcgtcag tgtaattttg atctgattta attgtattat tatccgcaac actgtctttt
    32401 aatttcgtca tcgcagtatt taaagccgta atgttagact taattgtttc aagcgcagca
    32461 tgattttgag cagcattaat ttgttgcgtg aaattatttt gttgtgcttg gtttaagtta
    32521 gatgcaccat gtaatgttgt taacgctggc tgtctatcta atgttaaacc ttgacgtgct
    32581 gtatttaatt gatccttagc ttggtttgcc tctgtcactt tatcattgat attttggaca
    32641 gttgggttgc cattcaacac ttgattaatt tcagttaact tttgacgcgc tgcttgtaat
    32701 ttttggttgt acgcatttaa cgaagcggtc gtcatacctg ttgtatccgt tggttgatta
    32761 atgctttgtt ctaattgcgt tttcgcagtt tgaagcggcg ctttatctgg tgttaaagct
    32821 tgacgtgcat gatctaaatc agatttagct tgatttgctg tagacgtatt tgtattaatt
    32881 tgttctacag tcggtgaacc tgctaatact tgattgattt gttgaatctt attacgtgca
    32941 gctgttaact tcgcattgta tgcatttata gagtcttgtg tcataccagt tgtacttgtt
    33001 tgcgtgtcaa tactatgttg tagttgattt ttcgcatttt ctaaaggcgc tttatcgact
    33061 gttaagccat tacgtgcttg atcaagtgct gatttagcgg cattcgctgc tgtcacgttt
    33121 tgacgtattg tcgcaacatc tggatgtgag gctaatacac gatcaatttc ttgaatttta
    33181 gttctagctg ctgaaagttt ttcattaaat gctgcaatag atgcgcttgt cataccagtc
    33241 gtactcgttg gctgatcaat atcattttgc aactgagttt ttgcagtttg taatggtgcc
    33301 aagtctggag ttaatccagc aattgcttgt tttaagctat tatatttttc ttctactttt
    33361 gttttttctg cggcaatttg ttggtcagtc gcatcaccat tgttaataac attttgtgca
    33421 tttgttgatt ctgtttgacc cgcacgttta gcattttcat atgcttggat tgatgattgt
    33481 gtcataccat cagtagttac tgatttattg atttcttcat caagtttcgt cttagcagtt
    33541 tttaaagcac tattatcagc taaaggtact aattgattaa ttgcttgcgt taatcgctca
    33601 ttgacacgat ttacatttgt taacgcagat tgcacttctt gtactgttct tattggcttt
    33661 tgaataatag cattagcgct atttttagca cttgttaagt cactttgaag tgcacgaatc
    33721 gaattattgt aagcagtaat acttgccggc ttcttaccag tcgttgtacc tgtgcgattc
    33781 aattgttgca ctgcttgctc taaggcatgt gtatctgcag ttaaatcatg tttcgcttgg
    33841 tttaatgctg ttaatgcgtt atcgacacga tgtttttcat ctgaaatttg ttgtgcagtt
    33901 gcatcgccat tgtcaataac acgttgagct gcagttattt cagtttctgc ttcacgcttc
    33961 ttcgcattat agttatcaat actttgttgc gtcataccag cagttgatgg tacttggttc
    34021 acagaacttt gtaagttatt tttagacgtt actaattggc tattatcttc tttattttga
    34081 agtaatgctt tagcttgatc aatcttagtt tgtgctgcac gaactttagt tagtgcgtca
    34141 gaaacttgtt gtggtgttgc acgctcatta ttaatcactt gttgtgcttc tgttttcgca
    34201 gtattgattt gttgttgcgc attatgcatt gcattattgt actgcgtaat tgtacctggc
    34261 tttttacctt cagtgcttac tggatcatct aaatgatttt tagctgtgat taactcacgt
    34321 ttatccgctt ttgtgaaaat ggactcttgt acttcttcag tactaccatc attgtaagtt
    34381 actgtcacag gaatcgtcgt tgtgctacca ccagctaaat tagtaggcat tgctgtgcca
    34441 tttttaatcg ttgcagtacg tttattagca acttgaactg cattgttaat ttcagctgct
    34501 gttacatttg aaccataatc tttcacaatt tcagttgtgt tgacagtatg agctgccggt
    34561 gcagttaatg tacttggatt actacttact gagtgacctg tacctgcttt cggagtaatt
    34621 gtgattgatg aatttggttt tatagtattg gcattgaacg tcactttacc agtttgtgca
    34681 tctaacgtta catagtcagg cttattcgca attgtccatt gattattttg accacgaaca
    34741 acattaattg tcttactatg ttctgcacca ttacccactt tttcagtgta agcaatatcc
    34801 attgcttgag ttggattaat taaatgtcct gatggattat taggcgtgat atcaatatga
    34861 ccattttgcc aaatcttagt agtcgcttgg ttcggttgtg gtgcgacaac tgtgaaatca
    34921 tcactacgtt gctcatcact cactgtctct ccgcttcctt gcgttgcaac aacttgaatt
    34981 gtatcagcag ggttgaaagt acctgctgca acagtaatac cattattagt tccagcaata
    35041 cctgctacag ttgctgcaga tgcttctttc acccatggac tcgtattatt gcgacgtgta
    35101 aatgtcgtca caacgttacc attacgttta ataactaatt tatcagcgta tgtcgttacg
    35161 ttaccggcat gtgtattcac tgtttggttt gctccaggtg caattgtaat cgctcctgcc
    35221 gctgtttcag tcacagttgg tttcgctggt tgcacatctt ttactacaaa tttcgctggt
    35281 aaagatgttg caaaagtatg tccgttatag atgacgtcat attttgcgtt aacgacttta
    35341 gccacattcg gtttattcat agctgaccag tttgcgtcat ttgtacctgt agtatcacga
    35401 ttccatttat acgtaaatcc atctgttggt aaaccagtag cattttgcat atgtgcatat
    35461 cctgatgctt gcgtaccact tgctaaagtg cctccaaccg ttgtcgtata agtagtttga
    35521 gggaattcaa attgatatac attaacagta acaggaactc gtttagcagc tgaaatacct
    35581 ggatatgtga catcgacatt taaatgttgc acgcctgctt gttggttatt tggttgttgt
    35641 ctatttgccc atgctgcagt gataccattt gtgtttgtat ttggatcaaa tgtaatgtag
    35701 ttcatcgcat ccgttccatt agtcaaattt tgacctttca catcacgtga tggcgccttt
    35761 gcattagcaa ctggataaac tttgactgga acttcaacat tacgcgtacc ttgaccatta
    35821 ggtaatgtta caaccgcagt tttatgagtg ttaccgactg tattcttcca tgtatctgga
    35881 ctatcatgcc atgcaaccgt tgccccatgt ggcgggtttt gaataaatct ttctacgtgt
    35941 ccgaaatcaa aaccgtcgcc acctttaata aatacagcgc cttcagtagt tgcttgtaat
    36001 tgtggtgtca ctgttactgt tgcactgtca tttgaatcaa cagattcatt tcttgttact
    36061 gtaacaactt gaccatgttc gtcttgcgtc gtatacgtca cattgttcat tgaaattgaa
    36121 gattttgctt taattccgcc atttggtaac gcgtcactta ctgtcacaac cgaactaaaa
    36181 ccgctaccat gagtaatatt tgtgacattt aatggtgtat tatctgcttt aaataatttt
    36241 actgacgagt tatttaatac gttattaact ttaatttctt ggtttgtaag acctgcttta
    36301 tatgtcacag agtttgcgtc aattctaggt gggtcaggtt taactttaga taaaatagtt
    36361 aaatcttctg tttgtctatt atcatatgtc actctaataa gacgagtaga tatgcctaca
    36421 acatctttgt taggaccata tgttccaaca aattggaaat tcatatgttg cgcatttgtc
    36481 gaccaagaat tatttactgg cttaacatat tgtttcgcat catacatatc tgaaacacct
    36541 gggtataaaa cacctctggc tgtttcaaag acatgtttcg gtacagttct tactacttta
    36601 tatgttgctg ttttcgtaat cggcgttgtt tcgccatcaa ttaagatatg tgcagttaca
    36661 gttatatctt caccaatacg tgtattatct ttatttggcg cttgtccact tacccatgta
    36721 atagttgcat ctgcaatgtc actaccatta gataacttaa agtaatctga tgcactagaa
    36781 ccattacctg ctgggaagtc ttggccttgt acagtgtaat gcgaatgtgc aacgatttct
    36841 ggaatgacat gctttacagg tacagtcact gttgatgttg ttccatcttg ataagtaaca
    36901 gttactgtga cattggcatt atttccagta cgactgacat tactaactgt tttactagtg
    36961 atttcattcg cacttgctct tgcataactt ctatttggta ctgtttcagt aaacgttaac
    37021 gaattaataa ttgtcgtttg atcagcttgt gatactgtcg cattattcga aatattggca
    37081 attctcacag gattagcagc cgttgatgaa gtaccaactc gatatttatc acgcaaaggt
    37141 ttcactgtta cattgaacga agttgtagct gtattgccgc ttgtatcagt tgctaataaa
    37201 ttgattgtct tattagttgc tgatgtcaca tttggtgccg ttgcagaaac atgttgatgg
    37261 ttattatcaa cagtacctgt aatttgtgat gtatttggta cagttacaga ttgcacacca
    37321 aagttatcat tcgctgtaat agtatttgta aatgtttcac ctgagaacac ttgatgatta
    37381 gtgtaattac ctactgaaat tgttggattc actaagtcac ttggtacaaa ataaatgtta
    37441 atagcgtcag tagtatttcc tgtattttga tttaaatgtt caacatattg ttttggacca
    37501 tatggcgtta agtataactg tgctttataa actgcatcac ttgcattatg tgtagaatta
    37561 ctttttacaa cgtggtcaat tgtaaatgcg ccagcaccat tagctgccgg ttcgtttacg
    37621 ttaacaacag ttgagttact atggtttgca cgagtatttg aatttgtaac aggttgccca
    37681 ccataaccgt ttgaagggtt gatgatgtct aacacttgaa tcacttgacc attcaacgta
    37741 aattgacgtt gaccatcagt tgtcgcattt gattgtgaat aaccagtcgt tctaaagtta
    37801 ggtcttccgc cagcttctgc ttgtgatttt tcattaccag taatgttatt tcgcaatcca
    37861 ggcactgtag ttgttgcttt taacattgtt aaaatatcat tcgtcgtaat ttgagtacct
    37921 aatgtagcat cataacgata aattaaagat ttatggtcat cagaccatga taaaccacca
    37981 tctgttggtc tgtttgcaat ttttgcattc gtccatgtca ctgtataatc ttgtgggaaa
    38041 ttaacccaac gcaagaaatt catattagct aggtttgacg cgaatgattt cgttaatcga
    38101 cctttattaa tatttactgt aatagtactt acattagcac cattaggtgt atttgtaact
    38161 gaaatatcac cttcggcaag cgtaattaca tctctatttg cattaataaa tgcttgtttt
    38221 acttttgaga tttcatcagt agttaaatca tatgttgatg tacgatatac acgttgatcg
    38281 ccttctggtt gtttcacatc gatgattttc aaactaatat agaagtcttc actatcttta
    38341 tgatacgcat ttgtagcagt tatatggtat aactgcgtgc ctgcgttcat ttgactaact
    38401 tcttgtgcag ttggaatagt tctatcagat gtaatatttg taaatgtgcc acttggaata
    38461 ggttgtccat tacttaaagt aaaatattgt ttaatatcac caatactaaa tgattgtcct
    38521 ttagcaatcg ttaccgttgc tgttttagca gcattgacag tttggtattc aggtttcaca
    38581 actggttcgt atgtcatcac atttgtagca tcaagcgttg tcgatgagcc atcacggtaa
    38641 tgtaatgtga cattaccatt attatcgaca gttactggat cagttgatgc taaatatcct
    38701 cttatatttt ggtttttatt cataaaggca gtaattatgc tttgcttttc atcattagat
    38761 acagcagtcg gattgactac tacaactttc tcagtatttc ctaatacaat cggatgagca
    38821 tcttcactaa ttttacctac atgaattgaa acatgtttag attgactatc atttgtcgta
    38881 ttattgacat tgtctgtcgc tgacacttta aatgtaatat cactgttaaa tgcctgattc
    38941 atactcactc tacctgtaat agataatgag ccattattct tatccgcttt tgttaaattt
    39001 gatgtccaac cattcggcaa tgtcgtaata gttgtatttt ttataccaga atgtctatca
    39061 gagacaccca ttgtaaagtt gacttcgtcg cctcgatagt atttggcatt tattccaaca
    39121 ggattattga ttgttggtgg aatgtcatct ggcaaaatag taacagcatt actcatatta
    39181 gatgcaacta ctttattata ataaactact gctctaattt gtttacctac aggtggtatg
    39241 aatacaacat aatcttgcgg tcgtatagta attgtattat gcaccccatc aggtgtacca
    39301 gtcgcaagta tcgtataacc agatggtatt tgatctgcct cactaccacc attcgtagtt
    39361 tgatttgttg gtgcaactaa ataaaccaat gccgatggat ccaacggtat tcccgaaata
    39421 ttaacaggca ctttttgtaa tgctgtacct cttaattgct cagctgtcgt tgtaattgtt
    39481 ggtgtatgtg gtttaacacg aatttcgaat aattgttctt tacgtccaaa acgtatcgtt
    39541 ccaaagtaat cccctggtaa taagttacca acaagttcaa tattgcgttc tctggcatta
    39601 ttaggtctat ttgtcgagtt ttcaatgtgt aatgtacgcg gaatacctgt aatttgccaa
    39661 tctttatctg taaaattctc attacttgtt aaattaatac gtaatggttg aatagatgtt
    39721 acgctaccac ctaaattcac ggaagcattc ccttggttac ctgcatatac atagaaaact
    39781 ggatgcccag cttgttttga aaatggtttt tcagcaatat tttgagtatc gtcattttta
    39841 aagaatttaa tattatgttg gatttcaggt aataccgtta agttaacagg aactaaagtt
    39901 tcactattac cataagcatc aggatagtcg aaaccaatca ttaatcttat tgggtttcta
    39961 gttcttgctg cttcttgtaa tttttgatca gtaaataatt gacttaattg atattcattt
    40021 aattgttggg ctccacccgg tctaaaagca ttagtatttg cattattcga ataaaatcct
    40081 aacactttat tactcggcgt cttaacataa cttaaaggat catcaacaaa atcatctata
    40141 tttaggtttg tactttctaa tacctttttc gtttgtacat ttccttgact cacttctaca
    40201 cgtttagttg tacctatgcg atatgtacga ttaaccactt gtcttaaacc attaagacgt
    40261 tgttgatggt cttgcgttga ttcgacgtat agttgactgt acataaataa ttgattgtac
    40321 tctaaagcac gcccacctgc tgcatagtac aatttattaa cagttggacc ttgtgtttta
    40381 aatgaaattg tataacttgc atcaccttga ccgatgaaag tataaacttt aggaacaccc
    40441 ggtgattcag caggtttttg accattaata tattcaaaat tttgtctacc aaaagcttta
    40501 gtagcaggtg tcgctccagc ttcactaaaa taatcactcg ctctagataa atcttctaaa
    40561 gaataaatat ggacagttgg ccagtcatat attacttggc cacttctatt tcttatttta
    40621 aagtcatgtg aacgatgagg atcatttact ccatattccc acatttgttg aagtggttta
    40681 tttgcacctg ctcctgctcc atgactccat tgtacatttg ttccatctga attaactgtt
    40741 acaaagtcag ttcttcctac tggcacttgg tctgctggta atgcaaacca aaagatcata
    40801 ttttgatgtg gagcatgtcc tttattaaac ttaataaccc attggtaccc gtctccagtt
    40861 tgtttcgctg tcataagagc aatttccttt gagtcagagg ccttcaatgt attccatggt
    40921 gtagttggta aattatatgg cgccccttta ctagaagcat tgatatatgt accaacagga
    40981 tagcccgaac catcattagc attttcaaca taatatgatg aatttgcatt ttgatagtct
    41041 gatactgatc taggctctgc agctcttgac tcaaccgaac gcgtttgaat tctatttgaa
    41101 cgtcggctag tctgttgacg ttgaggcatt gtacgaatta aagcaacagc ttgttccaat
    41161 tctttgtatg caagttgcaa gtctttattg tttattggat cattactgcc taataacgta
    41221 ttaatcttat caaaaatgcc ttttacatag ttaaatgtgc cagaatctgc attttcacgg
    41281 tcaataaagc gataatcatg ataatttgca tcaaaaaacg cttgtaattc atttcggtca
    41341 tgttgtacgt taccattatc agatgaatct gaagctgtta ctacatttgc ttcatgattt
    41401 tctacatgag ccgctctatt gtcgtttcca tttttattag cagattgact ttcgttttgt
    41461 ttatgcttac tttgttcttt atctggttgt gttgtcgcag ccgttgccga atctttcttt
    41521 gtatttacat tttgagatgc tggttgttca tcattagtag tagatgattg cgctactttt
    41581 tgatctacta aattagcttg actaatattt ggttgctcat tttcatgagt agcagataat
    41641 gattgaccat tttgtgaatt ttgagaattt tgtacttgag attctcgatt ctcagtctgt
    41701 tcattattac tttgttgttt ctgtttaacc acgcttgctg gttgatttgt ttcagcagca
    41761 tgtgcttgtg atgtattgaa tcctaaaaat accaatgtcg caatgacagt tgaaaatgta
    41821 ccaactgtat atttacgaat actaaacttt tgaattttat cacgataatt cacagctatt
    41881 gttcactcct tatcttgttg ttatgtctct gttgtgttgt gaatgtattt acttttgatt
    41941 gcttgtgtat tgtaatgctc attccattct catttaaaaa tttaaaacga tgacgattca
    42001 atacaatttc gcaatctatc caaaaagtat tgaaacacat tacaactatg tgttgaacaa
    42061 tttatttatg ctccagaata taataacaca aaatatattg taaggtaaat tgaatttgtg
    42121 acaatttata ataattataa ataaagtagc gtaaaagagt aataaaaaac caatccctat
    42181 gttcatttaa aataatgaca cataagaatc ggtttatatt tttgtttaat attgtagcga
    42241 cttgtatgat ttcaatttaa ttctcaatta tcataattga gtatcgtttt gtttaggcac
    42301 aagtaacaaa atgataacga atgataatat tcccatacct gcatttaacc ataatgcaat
    42361 cattgcacct gtataaatgt ttgtcatatt tgatacgatt gcatatactg caccactcaa
    42421 tgcgacgcca aatgctccac ctaatgcaga agccatttta tagatacctg cagcaacgcc
    42481 tactttttct aacggtgcat ttgcaattgc tgtatctgtt gatggtgtag catatatccc
    42541 tagtcctaaa ccaaagaata aataacctat aatacaacaa atgacataga atatttctgg
    42601 caagaaagtt aatgaaatga gacattctcc gacaataaga actcctgttc caattaacat
    42661 tggtttcttg catccgagtg tttgaagtaa cttttcacca acacgaatca taattagtac
    42721 cattactaaa taagtgattg ataaacttcc tgcttgcaat gaagaatatc ctaaacctct
    42781 ttgaacaaat gtgttggcta ctattaatgt tcctgcaaca ccatttaaca aaaagtttga
    42841 agctgttgca cctgtgtaag ctttattttt aaataattta aaatcgatta aaggatttgt
    42901 agcacgcttt tcaagaacta taaataaact aaaagatcca attgcaatag ctaataaagt
    42961 aataaaaaga agtgaggtta cacctaattc tgatccttta gtaattaaaa tatttaaact
    43021 gaggagcata atgactaaaa gaaccagacc tttaatgtca aatttattta gagaaatcga
    43081 tttagattta gtttcaggtg tgcctttaat aagaaacagt gcaattaatg aaattataat
    43141 tgataggatg aaaatccaac gccaacctaa aagcgttgca actgcacctc caaaaaatga
    43201 acaaacacca gagccgcccc atgagccaat tgaccaataa cttaaagcgc gttgtctatc
    43261 tttcccaatg taatatgact taataataga caaagttgca ggcataatac atgctgctga
    43321 aagtccttga attaatcttc ctataataag taataaagga atatttgaaa taatgattaa
    43381 taatgaacct aatatattta agataatacc aatgttcgtg agtttaattc tgccatattt
    43441 atcagcaaga ccacctgctc ctactacaaa cattcctgaa aataaagcag ttatactaac
    43501 ggcgatatta accgttccaa tatctgtatt gaaactatct tcaagtattg gtacaacatt
    43561 aaccaatgat tgtgcaaata gccaaaacgt tattacactt agaacaattc ctattaacaa
    43621 cttattattg ccttcaaatg cctctcttga cggcttttcc atctctattt gcctccctat
    43681 acttttgaaa aacagacaaa ttccataaca gaaatttgtc tgttccatag aataattgaa
    43741 tggtttttga ttttaatgtg taaaacttta aatttattag tagtccatcc atttattctc
    43801 aatgctagtt atcttacctt atgctgcttt ttgtttcttt ttatagtaat aaactggtat
    43861 accaagtgct gttattagaa ttccaatgat tgctaatata aattgtgtaa acagtgtatt
    43921 aattaataca aatgatcctg ccaaaatagc aattaaaggt atgatcggat ataacggtac
    43981 tttatatggt cgttccatat ttggttcacg ttttcttaaa attattaccg caacaaatga
    44041 catacaatag aacaaccaaa taacaaagat taacatattt gtaattgtat caaatgctcc
    44101 cattgacatc atgatgatag cgattataag ttgtataatt gcgccaaacc atggtgcgcc
    44161 agattttgtt aattttgcga ataaatggct aaatggcaat aattttcttt cagccattgc
    44221 atatggtacg cgcataccag tcatagtata gccattgatc gtaccataaa cagaaattaa
    44281 tataccgatt gtaataatct taccgccatt ttcaccaaat aatatttttg atgtatctga
    44341 agctgcattt aaattaccag caagtagttc tattggcaac gttaataaga atgtagcgtt
    44401 aattaataaa tacacagcca taatacaacc gataccaact gaaatcgcta aaggtaaatc
    44461 gcgtttagga tttttaagtt cccccgcaac atttcctaca tgaatccaac catcatatgc
    44521 aaacatagtt gctaataaac cactaccaat tgctgtaaag aagccatttc ctgaattacc
    44581 tgtagttgga attaatgaaa aagtgatatc tccagattga aaaataccaa aaattacaat
    44641 aacgatgatt ggaatcagtt taattactaa agtaactgat tgtaaaattc cgcctgcttt
    44701 tgaacctagg aaatttatca acacaataga taacgcagat gcgattgcta ttggtattaa
    44761 cgaacctata gataaatgga ataaattaat tagctgtgtc gcaaatacga tagacaatgc
    44821 tgctacgtta gctggaaaat aaataaatga ttgcgcccaa cctgatagga agccccagaa
    44881 atcaccgtat gtatattcta tatacttcgt taagccacct gtttcaggga ttgcagcagc
    44941 aagttctgct gctgttaacc ccgcacaaat ggtaatgatg ccgcctagga accatacaaa
    45001 caaggccatt cctgctgttc ctgttacttc tgttacgttt gatattttaa agaatactcc
    45061 tgaaccaata actgtcccca taacaatagc aaacgctgag aagaaaccta tatttttttg
    45121 taattcttta ccatttgaca taattacacc tcatttttaa gattaaatgt tttaacctac
    45181 cacacccttg cttgtatctg caatattcag tccatgttca atgacacctg aaactctagt
    45241 taagtcaaca ttcccgcctg aaactaatgc aacaacattt ttatcttcaa gccatttatt
    45301 gtttattttt ccacttaaaa ttgcagctgt tggtaatgcg cctgcacctt cagtaataat
    45361 tttggcacgc tgcattaaat ctttcatagc atgttcaatt tcttcttcag taacaagaat
    45421 aaattcatct actaaatgtt taactacttc atatgtttgt tcaccaggaa cttttacatc
    45481 acaaccatct gctattgtgc tatccactcg atgttcagtt aaatctctct tatagaaaga
    45541 ctcagccata ccatgaacat tctcagattg aacaccgata atatgaattg aagggttaaa
    45601 tgattttaat gcggtggcaa tacctgcaat taatcctcca ccgccaactg gtacgatgac
    45661 tgtattcaca ttccaaatat catctaaaat ttctaaacca attgttcctt ggcctgccat
    45721 tacaaactta tcgtcatatg gatgaacgat tgtcatgcca ttttctttcg ctaattcttc
    45781 catataaagt ctagtttcgt taaagttttt accttttaaa ataacctttg ccccatagcc
    45841 ttttgttgct tgttgtttcg cttgtggtgc tgtttcaggc attacaatcg ttgcatcaat
    45901 gcctaataat ttagctgtta aagcaacacc ttgtgcatgg ttcccagcag atgctgcgat
    45961 aatgcctttt tctttttgtt catctgttaa gtgattaatt ttattgctag cgcctctaaa
    46021 tttaaaagat cctgtgaatt gcatattttc taattttaga aatacattcc ctttagttat
    46081 actttggctt aaatacattg atttaattag aggtgttcga cgaataaatg gtttaatgct
    46141 tgctttagct tcttcgatat ctcctaaact tacaatatgt gctgtttgta atgtaactgt
    46201 gttggttgtc ataataatta catccttccg ttttgtaaat ttttagtcca aactataatg
    46261 ctatatttaa aatattcatt tcactcaatt tcatttaaat tactatctat tcgataacat
    46321 ttaatatttc tttataatct aggtcatgtg atgaagctaa tccttgattt gtcacttgtc
    46381 cttggtaaat gtttacacca gtacttaagg cttcattatc tttaattgct tgttctaagc
    46441 ctttgtcaca aatttctaat atataatcaa tatttccttg tgctaatgcc attgttgaag
    46501 ttcttgggac tgctcctggt tgatttggta caccataatg aatcacacct tcttcttcat
    46561 acactggatc agaaattgta gttggtctaa ttgtttcaat agttccacct tggtcaatag
    46621 ctatatcgat taatactgaa ccttttttca ttgatttaac catctcacga gtaaccaatt
    46681 ttggcggttt cgcacctgga attaaaattg tagaaataaa tacatctgct ttcttaattt
    46741 gttctgctaa attttctggt gttgatttga ctactgtgac atctttttct gcatacatat
    46801 cttcaagata tttaatgcgg tcatcgttta actcgataat gattacttta gcatttagtc
    46861 ccaaggcaac atttgctgca tttgttgctg ctactccacc accgaaaatc acatatgtac
    46921 taccaggtat atccacattt tcatgtacac cagtcactaa agtaccttga ccaccatgtt
    46981 gtgcttcaga gtagtaagct cccataattg ctgagcgttg acctgctata gcactcattg
    47041 gcgctaataa ttctgctttt ccatttttta taatggtttc accactaatc gcagttacac
    47101 caacttcttg cattttttct actatttctt ttgaagatgc tagatgtaaa aatccccaga
    47161 taatttgatt ctttttgaaa tattgatatt cgctttcatg aggttctttt actttgataa
    47221 caagatcagc ttcccatgct tgttcgtgag ttacgatctt agcgccttct ttttcataca
    47281 tatcgttaga aaatcctgaa ccaatgccag catttttttc aacaattact ttatgtcctg
    47341 catccgttaa cttacgcaca ttttcgggtg tgcaagctac acgtccttca ccttgtttta
    47401 attctttgac tactgcaact aacatttgca acactcctct ttcatttgtg aataatttca
    47461 caattatttt atcctttggt tatgaatatg taaataccat taattaatta ttcatatttt
    47521 atctataatt tgttaataaa tatttaatat attattctta ttcttaaata ttatgtgcaa
    47581 ttataccact atatttattt cttatactcc atttgattta tagttgtatt aatacattga
    47641 ctcaaaaact aattaatcaa atatgttttt tagattaata aagttgtaac tgtactattt
    47701 tgtagtgtat ggtaatttat ttggatgaaa tataattctt tatactctat tgattaatca
    47761 aatatgtatc tatcaaaatt cggcttattt attcactctg acatatccaa atacgcaaaa
    47821 agactatttc taccttgtat cgatagaaat agtcttttta tacttaattg gtttaatttt
    47881 tcataaaatc ttatactttt taaaaatggg atgaaatata ttttcctgaa acatgtaatt
    47941 catgttcgtt acaaattgat aaaatatgat taagcgttgt tgcaaatgac attttctcaa
    48001 aaagcgcttc actatcaatt ggtacttgtg tgtgaatttc agccagtcgc ttcgataaat
    48061 atagttcatc taaattgtca ttaatcttat tacgttgccc agctgataat gcatcaatgt
    48121 tttcaaccac attttcaacg ctttgatatt gctgaattaa cttaatagcc gttttctcgc
    48181 caataccttt aacacccgca tatccatctg ctgtatcacc cataaacgct ttaatatcga
    48241 ttagttgttg tggttcaagg gcatattctt cgttaaaacg atgtaatgta tatctattat
    48301 aaatgttaaa acctttttta attagccaaa cttcaacatt gtcattaata cattgcagta
    48361 aatctttgtc gcccgtaata atatagacat cgttatcagt tgaatattgt tgtgctaatg
    48421 tacctataac atcatccgct tcatagtttt taacgccaat atttacaaag ccaaattgct
    48481 ctgaaatttc tttaacataa tcaaattgtg gtatcaattc ttctggtggt gcagaacgat
    48541 tttgcttata accatcaaac atatcatttc taaaagttga ttgtcccata tcccaacata
    48601 cagctacatg tgtagggcgt atttcatgta ttgccgaaaa gatatgacgc acaaatcctt
    48661 gtattccatt tgtaggttca ccttgtgaat tgtacataaa ttgtttatga agacttgtag
    48721 catagaaatg tctaaataat agcgccatac catctacaag taatatttta ttaggcattt
    48781 aaaacgttcc ttctttctta atataaaata gttttcagtt gttgatgttt atctttcaat
    48841 tgattgataa gtgttgtatc taaatcaaat gctaataaat cgtttatttg cgtttgaata
    48901 ttagcttcaa aactttcaaa ttgcgattca gcttctacag ccatttgctt tacatataat
    48961 tcaagctgtt gcctcaataa tcccatttgt ggttgtaata attctaacgt actttgacaa
    49021 attgattcat gtatatctct ttgcccattt ggattcaaaa ttttacgttt tgttaattgt
    49081 ttaggcaatg catttagcat atcgttgaaa tcgatgtgca ataatggttg ctctatatta
    49141 gctgattcaa agttaaactg aggattaata atgacatgta aatctgctaa ttgttgaacg
    49201 attggtgcaa tttgctcagt gagttgctta ttaaagtatt tttttatacg ttctgtaatt
    49261 aaagattgct ctaaaaacaa tcgttgatga atttgatcta agtatactct cgtagacact
    49321 ttcttttctt cattaaaatc actattttgc gtcatttgag aattaaacac tgatttaaca
    49381 tcgtcaagta gttgtagttt taaacgggca tttaaatgat aaacttgttc ttcaacttcg
    49441 ttgtctgtat gttgtaacgt cgcatcaatt aattgatgtt gtaaacgctg tttatcttta
    49501 taatctgtta acctttgttg gcgacgtgaa atatcagctt tatttgtttc aaattctgta
    49561 atcatctcta cataagaacg atccatttgt tgaagctgat gaatcatttg ttgttctaaa
    49621 attgatttag attcaacatc aacaaattgt tgtatgcttt gttttaattg atcaatgccc
    49681 ttatcttcag cttgcaatgc atttcgactt gatacagcaa aaatgtctga ttgtaagtgt
    49741 acttgtctta atgcatctga tacatatgtt tcaactgctt caagatcatc ttgactttct
    49801 gctaaatcag cagcattaat taccattttt aatgcttggt tttcattcaa ctggttcata
    49861 tctttcatgt gttctataaa cgctttgtca ttatcagtaa atgaatgatt aaaataactt
    49921 acatacaata ttaagtctga agaagttaaa atttgttcgg tttcatttgt atgcctttgg
    49981 ttatttgagt gtagccctaa tgaatcaaca attattttac cttttaacca atcatgcatt
    50041 aatgcaatgt gtactgtctt aacaaatgtt gcatattcat cttctgcact ccactttttc
    50101 aattcctgtt gattaatggc atgtttttct ccattttcca acatattgac atacaattta
    50161 taatgttttt caactgcctg aataaaagcg agttgatttt tatttaaatg tgattttaac
    50221 ttttctaaat ctgaattaat aaagtcttct atagtcgaat aagacatatc ttggtattca
    50281 actactgcat taatttcatc taataattgc gattgtgatt ttagcgttat ataactctcg
    50341 tctccataag atatttctgt agtagctgct gtagtaggat ttggagaact gactaaaata
    50401 tgctcgccta ataatgcatt tatcaaacta cttttaccag cactaaatgt tccaaataca
    50461 cctattttta ttaatttatt atctatacgt gttaatgttt cgtggatatc ttgctttgta
    50521 cgcttgaaca aaggcacatc cgaaattata tcaagcgctt tttgaatatc tatagacata
    50581 tttgttgtag ctgtattttg atttagctgc tgattatcac gattatcctg agcattatca
    50641 gtagccactt gatatgttgt ctcttgtcga cctattaatt tatctagaga ttcatctaaa
    50701 tgaatatagt agtgacgata attcttagtc gtcaatgact gacgcagctc atttaattct
    50761 gtataacgtt gatattcttt taaatcatca ctttcttctg ttggtaattc atctgcctgc
    50821 acattttcta ttatttcttt aaaaattggt gttgattgtt gttcaacata tttcttaatg
    50881 gctttaacca cttcgtctga aaatgtaagt acataagtat tgctgattga tgtttgtggt
    50941 tgatataaac cttcaatcat ttctggctta acgtcataat gctgatttaa tactttatct
    51001 gaagcttctt ttttattgat aaaacgcgta acaaatgaca tatcttctcg cattggttga
    51061 cgaatttgtt gattaacatg ttcttgtaac gcatctgtcg ctgttaataa tcgctgttgt
    51121 tgaatttgta gttttttctt ctttttatta aaaaatccgc caacattaaa gtcttgagac
    51181 atactttcca aataacttcg taacatttct ctcatattat gcggcataat gtatgcattt
    51241 tctaaaatat ttttacgctt attctttaaa aatgccatta attcatctgg attatttaaa
    51301 agttgtgcct cttcactaat tgcttgatgt tgttgactat ttaaaaatgc ttgttcaaac
    51361 tccgcttctt cgatacctaa atcttctagt acttcctgaa tttcagactg aatgtaatct
    51421 agctgagctt cggtaatgta ttcaaccgtt cttgatgtat aatcctctat tgtctctcta
    51481 tgttgatcta atgaaattag ataacttgat aatacttcaa gttcattttc agggtgatca
    51541 aatttagata cataaaaggt gcgttctaat ttaatacccc aatctgcaat tgatttttca
    51601 actcgagatt taaacgtaga gaatgacaat tcatcgtctt gatgcttgtc aatttgatta
    51661 atgataaaca caacaggtat tccaacatca tttatatgct tcataaactt aaagttaagt
    51721 tcagattgaa cgtggttata gtcaaccgta taaaatatca tattacttgt atacatatat
    51781 tgttctgtta ttgactgatg tgatgcaaca tttgaatcaa cacctggtgt atcttgcaac
    51841 gtaaacccat tttcaaattt agctgattga aaattaattt ctacagattc aacgtcgaca
    51901 ttttggcgat tcatttccct tacttcatca taattagata atttggcata cgtttgattc
    51961 ggcaaattag caataatatc gtgattgtct gaaactgaca caatagcagt attacttgtc
    52021 gttggtacag gtgaacttgg taagatatct tgttcaatta ataaatttat cagtgtcgat
    52081 ttacctgcag aaaaatgtcc aacgaacgaa catgtatatt gctgcaaata tactttctta
    52141 attacttggt taattgtatg taaaagtgct tcatttcgcg acttttcaac ttcttttttt
    52201 aatttatata aaagatctaa ttgttcttta ttaatcatgt tactttcccc tttggttcgt
    52261 tcacatagaa atgcttgctt gttacaacga attcatacaa tatcgatgtc catttaaaca
    52321 tagatcgtta aatggctcgc attgtattat gtttaaattt tatcatagct gcttgataaa
    52381 acatacgaat aatcaatttt cttcaaacat tccaacttaa aagttcattt ttctaaaaat
    52441 cacaaatcat cgttaaattt ttctagaaaa taaatattgt cacagtttgt tcacacacat
    52501 cacagaattg acaccgtata atagccaatt gggagggaaa tatgaaaaat acatttaatc
    52561 cattacaaag attacatttc tatgcagcaa tatttattgc tccactgtta atcactttaa
    52621 ccatttcggg cattggttac ttgttctttc cagaagttga aaataatatt tataagaatg
    52681 agttttttgg tgacagtgat gtaaaaacgc atcaaacatt aaatgatgca gtacatcaag
    52741 ttgaacaaca atatgaagga ttctttgtaa gtaaagttag catacttgat gaaccatata
    52801 acaaacgaat tacactgagt gatatggcag gaaatcaacg ttacgtcttt ctagatcata
    52861 acaatcaatt tgttgcagat caaaatgcga aacatacgta ttctaatgtg atgcgaagta
    52921 tacatagttc tttgtttact gaaaatacta ttattaatta tttagtagag ttaaccgcat
    52981 gttggatgat attcatgatt ttatctggta cttatttact cattaagaag catttaattt
    53041 ctaacaaaag taaggcactt cgttggcaaa agtggcacgc aatgattgga gttatcattg
    53101 caattccagt atttgtatta gtcttaactg gattgccatg gtctggtttt atgggcagta
    53161 aaattgccgg tatgatggac acaaacggtg cccttggtca aggtgaatta gcgattaatc
    53221 cacctaaatc agatttgaac gaattacctt gggctacacg taaaaataaa cagccagctt
    53281 catccgaaaa aggttcaagt ggtcatcatg gtaatgcagc aatgcctcaa accaaattag
    53341 attatcaaat atctattgat aaggtcgttg aacaggcgca aaaagctggt attaaaaagc
    53401 cgttttcaat cgtatatcca agtgataaaa atggtacctt tattgtatct aatactagta
    53461 attcaggtgt tactgggcta gatgtatcac catacaagga acaaacactt tatttcgatc
    53521 aatatagcgg taaaaagcta ggtacgatta aatatgatga ctacggtatt attgctaaat
    53581 ggtttacatg gggcattccg cttcacgaag gtcatttatt cggcatttta aataaaatca
    53641 ttaatttatt tgtatgtatc gctttattag tagccattgg catggggttt gtctcttgga
    53701 taaagcgtac aaaaaatact gcaataaaag taccacatcg cgtaaaaaaa ccagcatcta
    53761 tatcactcat aatatgttta a
//
LOCUS       DAKTLH010000003        52332 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.003, whole genome shotgun sequence.
ACCESSION   DAKTLH010000003 DAKTLH010000000
VERSION     DAKTLH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 52332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52332
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.003"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            33..296
                     /locus_tag="O0394_000086"
     CDS             33..296
                     /locus_tag="O0394_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0498203.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            complement(434..1006)
                     /locus_tag="O0394_000087"
     CDS             complement(434..1006)
                     /locus_tag="O0394_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCY0498204.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTMNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(1187..1555)
                     /locus_tag="O0394_000088"
     CDS             complement(1187..1555)
                     /locus_tag="O0394_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCY0498205.1"
                     /translation="MKTILKTITYLVLTIIGAYAALFILKTIDSHGITDQFNPLVKKD
                     DSYVKTTEVSTRMDDQLRSYSQSVFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            complement(1924..2535)
                     /locus_tag="O0394_000089"
     CDS             complement(1924..2535)
                     /locus_tag="O0394_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCY0498206.1"
                     /translation="MFKKYDSKNSIVLKSILSLGIIYGGTFGIYPKADASTQNSSSVQ
                     DKQLQKVEEVPNNSEKALVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLE
                     GTYTVAGRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV"
     gene            2735..3442
                     /locus_tag="O0394_000090"
                     /pseudo
     CDS             2735..3442
                     /locus_tag="O0394_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505297.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            3481..4419
                     /locus_tag="O0394_000091"
     CDS             3481..4419
                     /locus_tag="O0394_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCY0498207.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNYFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHLTLDIEHSLKYLVLKLITENNQEDGY
                     KIIDEFLCIDKSYSNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPEKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQPIIAN
                     LNYDSRLPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQKRYKRNESYYNNALAIKRFFIALDKIIDFNRPKV"
     gene            complement(4685..4927)
                     /gene="rpsR"
                     /locus_tag="O0394_000092"
     CDS             complement(4685..4927)
                     /gene="rpsR"
                     /locus_tag="O0394_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCY0498208.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(4979..5482)
                     /gene="ssb"
                     /locus_tag="O0394_000093"
     CDS             complement(4979..5482)
                     /gene="ssb"
                     /locus_tag="O0394_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0498209.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            complement(5503..5799)
                     /locus_tag="O0394_000094"
     CDS             complement(5503..5799)
                     /locus_tag="O0394_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCY0498210.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            6043..6234
                     /locus_tag="O0394_000095"
     CDS             6043..6234
                     /locus_tag="O0394_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498211.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            complement(6320..7417)
                     /gene="ychF"
                     /locus_tag="O0394_000096"
     CDS             complement(6320..7417)
                     /gene="ychF"
                     /locus_tag="O0394_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCY0498212.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(7429..7632)
                     /locus_tag="O0394_000097"
     CDS             complement(7429..7632)
                     /locus_tag="O0394_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCY0498213.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIESHDDTQR"
     gene            complement(7662..8543)
                     /locus_tag="O0394_000098"
     CDS             complement(7662..8543)
                     /locus_tag="O0394_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCY0498214.1"
                     /translation="MNQVMNIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVEENIDNVDKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAIEDTRVILRISAETIPGEGFAGARIIRKEVQKMFLQEGI
                     KTPQPIMTAYNHSENGV"
     gene            complement(8700..9545)
                     /locus_tag="O0394_000099"
     CDS             complement(8700..9545)
                     /locus_tag="O0394_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCY0498215.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQEDFVRIIEQLN
                     RRYGK"
     misc_binding    9774..10143
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10209..11312
                     /locus_tag="O0394_000100"
     CDS             10209..11312
                     /locus_tag="O0394_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0498216.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     gene            11309..12469
                     /locus_tag="O0394_000101"
     CDS             11309..12469
                     /locus_tag="O0394_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HCY0498217.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISD
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            12438..14279
                     /locus_tag="O0394_000102"
     CDS             12438..14279
                     /locus_tag="O0394_000102"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HCY0498218.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAFLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLYGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            14276..16504
                     /gene="metE"
                     /locus_tag="O0394_000103"
     CDS             14276..16504
                     /gene="metE"
                     /locus_tag="O0394_000103"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCY0498219.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGIGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            16547..17299
                     /locus_tag="O0394_000104"
     CDS             16547..17299
                     /locus_tag="O0394_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HCY0498220.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIEKFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(17382..18563)
                     /locus_tag="O0394_000105"
     CDS             complement(17382..18563)
                     /locus_tag="O0394_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCY0498221.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            19102..20235
                     /locus_tag="O0394_000106"
     CDS             19102..20235
                     /locus_tag="O0394_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HCY0498222.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFASVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLTSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISYSLGIL
                     VMSYIVSLLFKGHDHVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            complement(20602..21231)
                     /locus_tag="O0394_000107"
     CDS             complement(20602..21231)
                     /locus_tag="O0394_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HCY0498223.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMICASILLLISFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIAYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(21231..22073)
                     /locus_tag="O0394_000108"
     CDS             complement(21231..22073)
                     /locus_tag="O0394_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0498224.1"
                     /translation="MTTLLNVDSVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSITFFGEEHTVDDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYMTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMEILEDFVAQGGGILISSHISEDIEHLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTEDKDVEIPKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            complement(22098..22811)
                     /locus_tag="O0394_000109"
     CDS             complement(22098..22811)
                     /locus_tag="O0394_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HCY0498225.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGFGIENLTFATYNNVV
                     VISIVATIIIILVEAIVLMNQRRALKYKQLVDKEVDIDATDQYELLANRYVLNGSILS
                     ILQTVIAFLVLLIFVVGHAAANAILFFLIPFFASAIFNTQFTLFNRRFDDRIPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIVVIGLYSITTGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            complement(22808..23011)
                     /locus_tag="O0394_000110"
     CDS             complement(22808..23011)
                     /locus_tag="O0394_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0498226.1"
                     /translation="MRNRLKELRARDGLNQTQLAKQAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETVFIIEEDEA"
     gene            23252..23641
                     /locus_tag="O0394_000111"
     CDS             23252..23641
                     /locus_tag="O0394_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCY0498227.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSNHPVAQKSNKTIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLQDQAVISELIPQ"
     gene            23741..23965
                     /locus_tag="O0394_000112"
     CDS             23741..23965
                     /locus_tag="O0394_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HCY0498228.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            23982..24683
                     /gene="tatC"
                     /locus_tag="O0394_000113"
     CDS             23982..24683
                     /gene="tatC"
                     /locus_tag="O0394_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HCY0498229.1"
                     /translation="MVHFSELRHRLVKILLSFVITVIVVYVSSFWWMTPFITYITRAH
                     VSLHAFSFTEMIQIYVMIIFFIAFCFISPVMFYQLWAFIAPGLHNNERQFIYKYSFFS
                     VLLFCAGVAFAFYVGFPMIIQFALKLSLTLNISPVIGFKAYLIELIRWLFTFGLLFQL
                     PILFMGLAKFGLIDTTSLKHYRKYIYFACFVLASIIAPPDLTLNILLTLPLILLFEFS
                     MFIVKFTCRGKPPTH"
     gene            complement(24775..26487)
                     /locus_tag="O0394_000114"
     CDS             complement(24775..26487)
                     /locus_tag="O0394_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCY0498230.1"
                     /translation="MKHYLTKFVAMLITAAMVCSFGLLKSQAAEQQSISDVYSVITDA
                     KSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSESNAVKSDVRKLEDAKANDNQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSNTSANDQYHVSQLNDALEKAIKAIDDNQLSDADAALTHFIETWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDLNNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFIIGIALAIVILIIFALLFRFIVKLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQGAYIMVVAILIF
                     KFKK"
     gene            complement(26468..27697)
                     /gene="efeB"
                     /locus_tag="O0394_000115"
     CDS             complement(26468..27697)
                     /gene="efeB"
                     /locus_tag="O0394_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCY0498231.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKNNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            complement(27694..28548)
                     /locus_tag="O0394_000116"
     CDS             complement(27694..28548)
                     /locus_tag="O0394_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498232.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDIIPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            complement(28816..29361)
                     /locus_tag="O0394_000117"
     CDS             complement(28816..29361)
                     /locus_tag="O0394_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0498233.1"
                     /translation="MFGMKVNEQITLKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFADGNGFQCGIWYEGTLVGVIGLHEINHMHRKTSLGYYLDKE
                     FEGHGIMTQAVEALIKYCFEELDLNRIEISAAVNNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYDQK"
     gene            complement(29441..29725)
                     /locus_tag="O0394_000118"
     CDS             complement(29441..29725)
                     /locus_tag="O0394_000118"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498234.1"
                     /translation="MDINQSNRHYNAYWRGPNKEADEKSAYNNVQVGDGPQHREIGSV
                     ISTDNASWRGPNIENFEKKFYKQCKLAGPQHREFRKEILQAMQVGEGQQI"
     gene            29835..30830
                     /locus_tag="O0394_000119"
     CDS             29835..30830
                     /locus_tag="O0394_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCY0498235.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSKLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSALLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            complement(30890..31456)
                     /locus_tag="O0394_000120"
     CDS             complement(30890..31456)
                     /locus_tag="O0394_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCY0498236.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQSTMRTAKVQQQVFEEENNQYDF"
     gene            complement(31470..32531)
                     /locus_tag="O0394_000121"
     CDS             complement(31470..32531)
                     /locus_tag="O0394_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCY0498237.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPLKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNILPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASPKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            complement(32545..33471)
                     /locus_tag="O0394_000122"
     CDS             complement(32545..33471)
                     /locus_tag="O0394_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0498238.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLENNGI
                     KSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAVNTPQMSDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPVEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            33779..35137
                     /gene="glpT"
                     /locus_tag="O0394_000123"
     CDS             33779..35137
                     /gene="glpT"
                     /locus_tag="O0394_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCY0498239.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            complement(35220..35660)
                     /locus_tag="O0394_000124"
     CDS             complement(35220..35660)
                     /locus_tag="O0394_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HCY0498240.1"
                     /translation="MCKSKILLKNIFSEESEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKSGYFVTCWTKDEDNYNRPYKIEEFADYLIVAVIDDELNGYFLFPRELLV
                     EKGILASSKYQGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFFEY"
     gene            complement(35764..37119)
                     /gene="mepA"
                     /locus_tag="O0394_000125"
     CDS             complement(35764..37119)
                     /gene="mepA"
                     /locus_tag="O0394_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HCY0498241.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMVSMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMSAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMASLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            complement(37226..37645)
                     /gene="mepR"
                     /locus_tag="O0394_000126"
     CDS             complement(37226..37645)
                     /gene="mepR"
                     /locus_tag="O0394_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HCY0498242.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            37857..39812
                     /locus_tag="O0394_000127"
     CDS             37857..39812
                     /locus_tag="O0394_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCY0498243.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQATPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEA"
     gene            39817..40260
                     /locus_tag="O0394_000128"
     CDS             39817..40260
                     /locus_tag="O0394_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0498244.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            40262..40546
                     /locus_tag="O0394_000129"
     CDS             40262..40546
                     /locus_tag="O0394_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCY0498245.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            40561..41907
                     /locus_tag="O0394_000130"
     CDS             40561..41907
                     /locus_tag="O0394_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCY0498246.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAIAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLAVGIVFGNAVKYMGLFGAILFIIIVGATTILLKGRQKEQQ"
     gene            complement(42000..43025)
                     /locus_tag="O0394_000131"
     CDS             complement(42000..43025)
                     /locus_tag="O0394_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HCY0498247.1"
                     /translation="MNNKVLATGGTGFVGMRIISRLLEQGYDVQTTICDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAVDS
                     MAKYHLI"
     gene            complement(43340..44362)
                     /locus_tag="O0394_000132"
     CDS             complement(43340..44362)
                     /locus_tag="O0394_000132"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCY0498248.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGID
                     DIKEAKRYELTDADWEAIDELADKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIYGDFFGQGDIKDVEEALQGTKMTREDLTHQLKQLDIVYYFSNVTVEAL
                     VDMILS"
     gene            complement(44340..45284)
                     /locus_tag="O0394_000133"
     CDS             complement(44340..45284)
                     /locus_tag="O0394_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HCY0498249.1"
                     /translation="MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDVADYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLHRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITAALRNDSTTKNNNIGETEDVLNRTD"
     gene            complement(45274..46074)
                     /locus_tag="O0394_000134"
     CDS             complement(45274..46074)
                     /locus_tag="O0394_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HCY0498250.1"
                     /translation="METLKSNKARLEYLINDMHRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LSAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFNRDAE"
     gene            complement(46075..46407)
                     /locus_tag="O0394_000135"
     CDS             complement(46075..46407)
                     /locus_tag="O0394_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCY0498251.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            complement(46441..47442)
                     /locus_tag="O0394_000136"
     CDS             complement(46441..47442)
                     /locus_tag="O0394_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCY0498252.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEDFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEKVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            complement(47556..47648)
                     /locus_tag="O0394_000137"
     CDS             complement(47556..47648)
                     /locus_tag="O0394_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498253.1"
                     /translation="MESIFKIKLMNGICRSENMNMKKKNKGEKI"
     gene            47744..48943
                     /locus_tag="O0394_000138"
     CDS             47744..48943
                     /locus_tag="O0394_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCY0498254.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLINWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            49016..49843
                     /locus_tag="O0394_000139"
     CDS             49016..49843
                     /locus_tag="O0394_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0498255.1"
                     /translation="MTEIKDKVITKDAFALPYTIIKAKNQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSVNETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTNSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMM"
     gene            complement(50085..52157)
                     /gene="lip2"
                     /locus_tag="O0394_000140"
     CDS             complement(50085..52157)
                     /gene="lip2"
                     /locus_tag="O0394_000140"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HCY0498256.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTSTN
                     AAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQSTQ
                     NSKTIRMQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQ
                     SNDVDKSQPSIPAQKVIPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTH
                     QPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAP
                     KTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKF
                     KVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKT
                     YKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGG
                     HNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFK
                     QLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSS
                     HTGPLGYENPDLGTFFLMATTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVTN
                     DEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATES
                     KGTQLKAS"
BASE COUNT        16614 a         9993 c         7882 g        17843 t
ORIGIN      
        1 aattttaaat caagaagcga ggttaatgaa caatgaatat gcatatttta tataacttac
       61 gaactaaaca taatttagaa attgacgaat tagcacagca attaaatgag aaatatggta
      121 ctaaatatga agcacatcaa atttgggaat gggagaatca tcaccatgaa cctaaattta
      181 aagatgccat gcatttagct gacttctttg atgcaccata tgaaatgttt ttagaaagta
      241 aggttaaaga atatcagaaa catttagaag aagtcgatat tcgcatggat aaatagatgc
      301 aaataaaccc tcacaacacg tttggcatat atcctttcaa atctatactg gatatattac
      361 attacgttgt gagggtattt tattaattaa tatgaattaa gacattttac aagcgttaat
      421 gcaacgaatc tttttagtga tcttgctcac tctttaatac tttaccgttc ttagcatcaa
      481 cagtaacttc ttgtttttta ttaccttttt tcaaatcgat attgtaaaca agtttgccat
      541 catctttttc aagtgaccat tctttaatat caccatcaaa ttctttttgt ccttctttaa
      601 tggctttttt gtaatctata gcatcgctat atttaaagtt atcattttca ttcattgtat
      661 cttctttttc agtctttttg ttaatcactt ttttattttt atcagcaaca agtacttctg
      721 actcttcacc agatttttgt tgcgtcactt tataagccca ttcaccatta gaattttcaa
      781 atgaaattcc tttcaacttt tggcctttgt aagtttcttc agcttttttc acagcatctt
      841 ctgggcttgt tttaacatct tttaaagcaa taacatcttt tgttgtatta gtgtcttgat
      901 tagtatttga ctctgttgat tttgtttcat cttttggagt atcattgcca catgcagtaa
      961 gcaccaccgc tgacattgat aacactgcta atgattttaa tttcataata tcactctctc
     1021 ttcctatttt tgaaactcat aacaaaagct tatatgctat atagattgta ttaccccttg
     1081 tttttaattt tattcataat tattacaaat atttttaaat taatcgtcat gcgttacttt
     1141 cgttcgtact ccttttataa atgaaccatg taatataagc atgctattat cgactcagtt
     1201 tgtctaatgc tttctttggt acttcttcct tttcaacttc ttcaaaagtt tctacatgat
     1261 gacctttatg tgtaattttc aagtatctat gcggtttaac atcaaatgta gcagtataca
     1321 ttaattgcgt ctctttccct tctttattaa aaacactttg actataactt cggagttgat
     1381 catccattct agtagacacc tctgtcgttt taacataaga atcatccttc tttactaatg
     1441 ggttaaattg atctgttata ccatgggagt ctattgtttt taaaatgaat aaagcagcat
     1501 aagcgccaat gatagtaagt acaagatatg ttattgtttt taaaatcgtt ttcaaaagat
     1561 cacctctgac aaaatataaa ataggatgta tctgaaaaca acttacataa ttgtaattaa
     1621 agtgtaagta ttaggagaaa atgatggtat ttgaatctga aagatacgaa tcgtttttaa
     1681 actaagttat tttatagaga ctttattatg aaattgaaag aagttaattt taaggttgtc
     1741 gaatgtaaaa acatattgat taaccataaa agagaaatta tttttgtaga tttcggcata
     1801 aacgtcatta aagaattttc tatatgatgg tgctaatcat aacaaagaaa gctaggcacc
     1861 tttagatacc taactttcaa agtattagcg ataaaacact atatatacta atttagctta
     1921 atttcaaact tgttcaatgt cattaacact tttcacaagt ttgaaagtta cattttttat
     1981 atcggctgta ttaattttta cattttccct atctttttgt agctctttat gcgactctaa
     2041 tgtatattta ccaccatctt ttgtatttat gacgatgtta cctttaggca aatgttctcc
     2101 catatacaag ccataggaaa tatgagcaaa tcttatgata tgatccaatt cttttaaagt
     2161 gacaacttct ttattaagag taatattcct cttaggtgta tacactctgc cagcaactgt
     2221 gtatgttcct tctaaatgta tacgaacttg gttttcattt aaaggtctct ctgaataaac
     2281 ccaattccta gatttattag attttccatt tattgtatcc ttgctgtatc tatcgtaaag
     2341 ttttttaacc aaagcttttt ctgaattatt tggtacttct tcaacttttt gtaattgttt
     2401 atcttgtaca cttgaggaat tttgtgttga cgcgtctgct tttggatata ttccaaatgt
     2461 tcccccatag atgataccta gcgatagaat agattttaat acgattgaat tttttgagtc
     2521 atattttttg aacatattta attacctcct tgatgtaaag ctttatttgc tacaattata
     2581 aaaataatag acgtgttcat gaattaaatt catcttaact cttgattaac tttaatttgc
     2641 taccactctg aatttaataa ctataaatcg tctacacata attggacaaa atctaagaga
     2701 ataaaatttg ttaatttaaa atagcaagca attcaaagtt atatgtgtaa tagataaaat
     2761 agatatccct atagtgatgc gttactagct aaacataata acacattaga agataatgaa
     2821 gttaaggagt tactggattg tttcgactat gtaattaagt ataaaaatat ccaacgacaa
     2881 aacgtaatta taaaatggta aaagctatgg tacagtttca aattgctaat gacatgcgta
     2941 tcggtgagct acttgcaata aagagagtaa atataaacta tgaagataaa acgctagata
     3001 tcgacggtaa agttaattgg ataactgaaa aaagacggga gcattcggag taaaggagac
     3061 aactgaaaga agtaatagct ataaggccac agggctcact acccaaagca tcgacttact
     3121 aagaacactt atgcttggaa tgataagttt attgatagag agtacatatt cacaaatacg
     3181 tctggtagcc ctatcgactc gaacaaaatt agccacatta ttaaaggggg gcgctgatat
     3241 tagttctatt aagaaaccta taacgacgca tacattacat cattcgcata tatctacact
     3301 tgctcaatta ggaattaact taaaagcaat gcaagagcat gtaggtcatt cagattataa
     3361 aaaaaatcta gagatataca cacatgttac taatcagatg gcgaaagata tgatgaataa
     3421 atttgaacga ttggggagtt aaaattggaa aaagatgata cactagcaga aattaagcct
     3481 atgctcaatt ttgatgagca aatagcgaaa ttaaaacaga tgaatatatt ttttaatatt
     3541 attgacaccg aaaaagcaaa tgaaattctt agaaaaaata attacttctt caaactagct
     3601 tatttccgaa aaaatttcga aaaaaagaat ggcggctatt tcatagaatt tgcttattta
     3661 tcagatttag caactataga tatgaaatta agatacacaa tgttgcattt aactttagat
     3721 attgaacata gtttaaagta tctagtctta aaactaataa cagaaaataa ccaagaagat
     3781 ggttataaaa taatagatga gttcttatgt attgataaat catatagcaa ttcaaatttt
     3841 gacacaaatt caagaacacc agaagaagtt atggaaacca aaatcaaaaa taaaaacgaa
     3901 atattcaagc atatgaataa acgaggacaa ctacccgaga agttgaataa atactatcaa
     3961 aatccacccg catgggtttg cattgaattc atgcaactag gtcaattcgt ttcgtttctc
     4021 aacttctatt acaagaagta caatgacgaa gaattgagag ttgctaatat tttaatgcct
     4081 ttagttaaaa atataagaaa caaatcagct cataaccaac ccatcatagc aaatctaaat
     4141 tatgacagta gattacctca atatttattt gaaaaaggga ataatatagg catatctaga
     4201 aacatgttcg gaataaaaaa tttcatagat actttcgcta cgctagaatt acataatcaa
     4261 gtttgtagta atgcaattat ccaagcaaga tatcacgatt tggaccaact tcaaaagcga
     4321 tataaaagaa acgaaagcta ttataataat gcattagcta tcaaaagatt ttttatagct
     4381 ttagataaaa ttattgactt caacagacca aaagtataaa ctatctagtg aggaaagaga
     4441 cttataggtc tcgcgagtta ttttaattcg tatgcaagaa aaagaagagc tatgcatttt
     4501 atttaaaatg cgtagttctt tttttatgca tctaaattca tattattttt gcaatataaa
     4561 catatctttg tgcaaattcc gaacacaaaa cattcacatc atcctttttt gccctttttc
     4621 tataccccaa aacacaaaaa gccccgtaag cctatgccta cggggtttga caataaatta
     4681 tatattattg ttcttcttta acatatggta ataatgccat atgacgagaa cgtttgatag
     4741 ctgtagtcaa catacgttga tatttagctg aagtaccagt tacacgacgt ggtaaaattt
     4801 taccgcgttc tgagataaaa cgttttaata attcagtgtc tttgtagtcg atatgtgtaa
     4861 taccatttgc tgtgaaatag catacttttt tacgacgacg tccgcctctt cttggtccac
     4921 ctgccatgat tgtgtgcctc ctttgataat tttttcgctt taatttcgtt aatttttatt
     4981 agaatggtaa gtcatcatca cttatatcaa tcggtccgtt tgcatttgca aatggattat
     5041 cagattgttt cgtgtttgat gaattattgt acgaattgtt ttgtcctgat tgttgaccac
     5101 cgaatccttg accgtaatct tggaattcat tttgttgacg ttggccacca ttttgttgcg
     5161 catttttagg ttcaaggaat tgaacgctat cacacacaac ttcagtaaca aacacacgac
     5221 gaccttcttg attttcataa ttacgggatt gtaagcgacc atctacacca gctaaactac
     5281 ctttagataa atagttattt acattatctg cttgtcttct aaaaacaaca cagttaataa
     5341 aatctgcttc gcgctcccct tgagcattcg tgaacgtacg atttactgca agagtgaatg
     5401 tcgctacact cacacctgag ggagtggttc tgtattccgg atctttcgtt aaacgaccta
     5461 ctaatacaac tctatttagc atttaaacgc cccctctaat tattacttgt cttcgtcttc
     5521 acgaataacc atgtaacgaa tgatatcgtc actgatttta gctagacgtt ggaattcgtc
     5581 agtagctttg ttgttatcag atttaacacg tacgatgttg tagaagccat ctttgaaatc
     5641 attgatttca taagctaggc gacgtttacc ccagtctttt gcttctaaaa cttctgcacc
     5701 ttcagtagct aagataccgt tgaaacgttc aactaacgct tttttagcat cttcctcaat
     5761 gtttgggcgt acgatgtaca taacttcata tgttctcatt ttatatttgc acctccttgt
     5821 ggtctatacg gcttatcaat cttaaaacag ataagcaagg aataattttc attactcaca
     5881 ataaagaatt atatcatgcg ccattacttt ttacaataat aattcaaact actcttcata
     5941 tcatttttga tattaattca tttgaaactt tctatgatat ttttaaaaat acacttcaca
     6001 aaagcgaaca tatgttctat aatagttgtg aggtggtaag gaatgaattt aaatattgat
     6061 tggtctaaag attttcaaga attccaagag atacttaata gtggtattca tcctgaatgg
     6121 ctttattgtg caaaggctaa tcttgtttta gagcctgctt atactggcga aggcaaacaa
     6181 tttttcagca ctcaagatat tattaacgcg agtaaaatta ttccattctt ttaataaatt
     6241 atacgcataa ttaaaagagg cgaacaattg attccgcctc ttctacatat atactattta
     6301 attaacttca ctctatcgtt tatacattaa atctgaaatg aacgatatcg ccatcttgca
     6361 taatatattc tttaccttct aatcgttgtc tgcccgcttc tttagcgcca ctttcgccac
     6421 catattgtac atagtcgtca taacttgtta cttcggcacg gataaatcca cgttcaaaat
     6481 cagtatgaat gataccagca cattgaggtg cagtcatacc ttgtttaaat gtccaagcac
     6541 gtacttcttg cacaccagca gtaaaatatg ttgataatcc taataattca taagttgttc
     6601 taattaatcg atctaatcct ggttcttcga tacctaaatc ttctaagaac atttctttat
     6661 cttcatcatc taatgtagca atttcttctt caatttttgc actaataaca atcacttcag
     6721 agtcttcttg cgctgcatat tcacgaatcg cttttacttt atcattatca tcatcaccaa
     6781 tttcatcttc accaacatta gcgatataaa gcattttttt agaagtcagt aattgcgctt
     6841 gattcaccca tttttgatct tcttcattaa agtcaatact acgagcgggt ttaccatttt
     6901 ctaaagcttc tttaatagtt gttaaaatac gtacttccat ttcagcagtc ttatcttttt
     6961 gacgtgctaa tttttcaatt ctaggcaaac gtttctcaac agattctaag tccgctagta
     7021 ctaattccat attaataact tcaatatcat caatagggtc tactcgacca gcaacatgag
     7081 ttacgttatc atcatcaaat gcacgaacga cctgacaaat cgcatctact tctctaatat
     7141 gtgataagaa tttattacct aacccttctc cctttgaagc acctttcaca ataccagcga
     7201 tatctgtaaa ttcaaatgta gtcggcaatg tctttttagg ttgaaccatt tcttctaatt
     7261 taagtaatct agcatctggc acttctacta tccctacatt aggatcaatc gtagcgaatg
     7321 gatagttcgc tgctaaagca cctgcttttg ttattgcatt aaataatgtt gatttaccaa
     7381 cgtttggcaa tccaacgata cctgctgtta aagccattaa tcattctcct atctttgtgt
     7441 atcatcatga gattcgatga tttttttaag ttttttatca aacgtttgac gtggaatcat
     7501 aatacttctt tgacaatttt cacatttaat tcttatgtct gcacccattc taataatctt
     7561 aaaacggttt gttccacacg catgttgttt tttcatttct actatatcat ttattccata
     7621 ttttgacgcc attaaatatg acctccatgt attataaact actaaacacc gttttcacta
     7681 tgattataag cagtcataat tggttgaggt gttttaatac cttcttgtaa aaacattttt
     7741 tgtacttctt tgcgaataat tcgagctcca gcaaatcctt cacctggaat tgtttctgca
     7801 gatattctca atattactct tgtatcttca atagcatcaa taccaataac aaccggatca
     7861 ctaacaaata agtaatattt actacgtaaa gaagtaaata gtttgtttag ttttttatca
     7921 acattatcaa tgttttcttc gacagacact ggaattttaa cgatagctgt accatttgta
     7981 attgagtagt tcgttatttc acccatacta ctatttggta aaattgttaa ttctcctgaa
     8041 attgtattga ttcgtgttga acgaagtcca attgatttaa ctgttccctc tgctacagtt
     8101 gtaccaccgt tatttatctt aacataatca cctacatcaa attggctttc aaatataata
     8161 aagaatcctg taattacgtc tttaactata gtttgagcac caaaacctac tgctaagcct
     8221 acgacaccag cactggcaat taccccttca acactaatgc caaatttact taaaatcgtc
     8281 gtaataacta taaaccaaac gatatacttc actacatttt gaacaagaga tattaaagtt
     8341 ttagagcgct ttttgttact ctttttactt ttattttgaa tcttaaatcc ctgttcaatc
     8401 attttattca gtattttaat aactatgagg gctacgataa tataaataac aatcatagcg
     8461 attttagttg caatgttttc atatgtttct atttttgtta atggctcaaa tagagatgaa
     8521 ataatattca tgacttgatt catgataagc gttactccct tctatatgat atatcaatat
     8581 tgtattcata tttttatata cttccaacct acgaaagtct caatatatct agaatcaaat
     8641 gttcactgat tacaatttat ttacacttgc acaaagagtt attatataaa attgtgtaac
     8701 tatttaccat acctacgatt taattgttca attattctca caaaatcttc ttgtgaatca
     8761 aactcaaatg agattttacc aaccgatttt tttattgata tatctacttt ggtaccatac
     8821 tgttctcgca actgtcgttc ttgctgcttt ataaatttag gcttagttat atctactttg
     8881 tctgtttccg actttgacga aacatttttt aattcattaa catggttttc taaatatctg
     8941 acactccact tttctttaac aacccgtttc gctaacctaa gcatttgttg ttcatcttta
     9001 attgccaata acgttcgtcc atgtgcactt gtcagtcgcc catcttttac catgtcagca
     9061 atctttttcg gcaaatgtaa taacctcaac atattcgcta tatacgggcg cgacttactc
     9121 aatcgtttcg ctacttcttg ttgtgtaatt ttcaaatctg tcatcaaacg ttgataactt
     9181 tcagcttctt caatcgcatt taagtcttct cgttgtaaat tttcgatgac cgccagttcc
     9241 atcatatctt catctgttaa atctttgata atcgctgata cgtattttag accagcaatt
     9301 ttcgaagctc taaaccttct ttcaccaact acaatgtaat aaccttgaac tgtttttcta
     9361 agcacaattg gttgcaaaat tccatattgc ttaattgaat ctgccaaatc atttaaatga
     9421 ttttcatcga aagtttttcg gggctgatac ggattcggtt tgatatccga tatatctatt
     9481 tgcttaattt gttcatcttt tgttttagta atacgttgat cttcactttt tgacaattca
     9541 ctcacatcac tttttatttg ataacaacag ttaaatatat tttatgtatt ctaaatgagt
     9601 cagacaacca ttaaccacac tttatttaaa aaataattca tttttcaact tcaagatacc
     9661 aattacaatt tcacaaacaa attaactttt aaatcaaaat tttctgaata attgttgaca
     9721 aatctttttt tactctgtaa gctaattaat atcttataac agtttaatga aacgtaaaca
     9781 caataaagag gaaagtaaaa cacaccctgc ttatacagag agtctttagt agctgagaga
     9841 agattttgaa agcgtgtttg aaaatggcct tggagtgttg atgccaatat gaggtgtcta
     9901 cgggttcgcc cgttatagcg atacagtatt aacattgatg ttaaatggcg tactggattc
     9961 tttacgcacg attttttgtt aataagtatg ggatagcaca ttactatatc cttacttact
    10021 gactttaatt gtgataattg ttcagtaagc atatttactt ttaatgcgta ctgaataagg
    10081 ttatttcagc gatggaataa caaataaagg tggtaccgcg aaacataagc tttcgtcctt
    10141 tttatccgat tcattcgggt acgaaggacg gaagcttttt ttattttttc ttataggagg
    10201 gtcttaatat gaaggataca cagttagccc aaatcacatt aaccgatgat tcaaccggtg
    10261 ctatagcgaa tccaatccat ttatctactg cctacaagca tccaaaacta ggacaatcga
    10321 caggttttga ttatacacgt actaaaaatc ctacacgctc aacatttgaa acctgttttg
    10381 ccaaacttga gcatggtatt gcatcattcg ctacatcaag tggaatgtca gccattcaat
    10441 taatatgtaa tctatttaaa cctcatgatg aaattttagt ttcattcgat ttatatggtg
    10501 gcacatttag attatttgaa ttttacgagc aacaatacga tatcaaattt aagtacgttg
    10561 attttacaga ttatgaacaa gttgaaaaag aaatcactga taaaacagtt gcattattca
    10621 ttgaaccaat atctaaccca caaatgattg ctattgatgt aaagccatac tatcaacttt
    10681 gtaaagctaa aggcttattg tcaattatcg acaatacttt tttaacacct tatctttcaa
    10741 caccactagc agaaggtgct gatatagtct tacattcagc cacgaaatat attggcggac
    10801 ataacgatgt actagcaggt gtcgtaaccg tcaaagatga atcactcgcg caacagttgt
    10861 ttgattttca caacatgact ggcgcaacac tttcaccaat agatagttat ttgttgttac
    10921 gtggacttaa aactttgcat ttacgcattg agcgtgcgca atcaaacgct agaaaacttg
    10981 ctaaaaaatg tcagtcactt caagcaattg acgaagtact atatagcggg caaactggca
    11041 tgcttagttt aagacttaac aaggcctata gcgtcgctaa attattagaa aatttagaca
    11101 tttgcatttt tgcagaaagt ttaggaggta ctgaaacatt agtgaccttc ccttacaccc
    11161 aaacacatgt tgatatgcca gatgctgaaa aagataaacg tggcattgat gagtatttaa
    11221 tccgcttatc actaggtgtt gaaaattatg aagacatcga acgcgatatc atccaagcat
    11281 tagataaagc tcagattgga gagattgtat gacactttca aaagagacag aagtgatatt
    11341 cgattggcgt agaggtgtgg aatatcattc agctaaccca ccactctatg attcttcaac
    11401 attccatcaa acaagtcttg gtggcgatgt taaatatgat tatgcacgaa gtggcaaccc
    11461 taaccgtgaa cttttagaag agaagttagc acgattagaa cagggaaaat tcgcttttgc
    11521 ttttgcatca ggtattgctg ctatttcagc agtacttttg actttcaaat ctggtgatca
    11581 tgtcatctta cccgatgatg tatatggcgg tacttttcgc ctcactgagc aaattttgaa
    11641 tcgatttaat attgaattta caaccgtaga tactacaaag ctcgaacaaa tcgagggtgc
    11701 cattcaatca aacacaaaat taatttatat cgaaacacca tcgaatccct gttttaaaat
    11761 tactgatatc aaagctgttt ctaaaatagc cgaaaagcat gaactactgg tagctgtgga
    11821 caatacattt atgacaccgt taggtcaatc acctttatta cttggcgctg atattgtcat
    11881 tcatagtgct acaaaatttt taagtggaca tagcgattta attgctggtg ctgtcataac
    11941 taataatgag gcaattagtg acgctcttta tttaatacaa aatggtacag gcaatatgtt
    12001 atctgctcag gatagctgga cacttgctaa acatttaaaa acttttccaa tcagatttaa
    12061 acaatctgtc gaaaacgctc aaaaaatcgt gtcattttta ataaagcaag atgaaatttc
    12121 agaagtttat tatccgggac tcactactgc tcatttagaa caagctaaaa atggcggtgc
    12181 cgttattggc tttcgtttag ctgatgagtc taaagcacaa caatttgtcg atgcactgac
    12241 attaccactc gtttcagtga gtctgggcgg tgttgaaacg atcctttcac atccagcaac
    12301 aatgtctcac gctgcactac ctgaagaagt gagacaagaa cgtggtatca ctttcggtct
    12361 attccgatta agtgttggtc tcgaagatcc tgatgaactc attgcagaca tcaaatacgc
    12421 attaaaggag gcattcaatg agtcaattcc tcacacaatt gaaagataat gttttagtag
    12481 ctgatggcgc tataggaacc attttatact ctgaaggatt agacacctgt ccagaagcat
    12541 ataatcttag ccatccagat aaagttgaac gcatccatcg ttcatatatt gaagccggtg
    12601 ctgatgtcat tcaaaccaat acttatggtg caaattttga aaagttaaaa cgattcggtc
    12661 ttgaagataa agttaaagca atacatcaag ccgccgttcg catcgcaaaa aaagcagcaa
    12721 ataaagatac gtatatatta ggcacagttg gtgggtttag aggtatcaaa caagaggata
    12781 tcagcttaca aactattctt tatcatactg aaattcaaat agacacctta attgaagaag
    12841 gcgttgacgc gctacttttc gaaacgtatt acgacctaga agagttaaca aatgtcattt
    12901 cacgaacgag aaagaaatac gacattccaa tcattgctca attaaccgct tcaaacacaa
    12961 attacttagt taatggtcag gcaatcaatg aaggattaaa acaactcgtt caatgtggtg
    13021 caaacatcgt gggactcaat tgtcatcatg gcccgcacca tatgcaagag tctttcacac
    13081 atattgaatt accagagcac gcattcttat cttgttatcc aaatgccagc ttattagata
    13141 ttgaaaatag tgaatttaag tatagtgaca atgcacaata tttcggtcaa gttgctcaaa
    13201 atctaattcg cgaaggtgtt cgtttaattg gtggttgctg tggtacaacg ccagagcaca
    13261 tcaaatttat taaagaatct attcagacac ttaaacctgt taatgacaaa aaagtgattc
    13321 cgataccaac gaaagcactt ttcaatccat ctcaaaataa agttagacaa tcattaacat
    13381 ctaaggttca agaacgtcca accgttatta tcgaattgga tacaccgaaa catttagaca
    13441 cggatagatt ttttgaaaat atcgctaaac ttgataaagc taatgtagat gcggtaacac
    13501 tcgcagataa ttcattggca actgtcagaa ttagcaatat tgctgctgct agcttaatta
    13561 agcaatatta caatattgaa ccactcgtac atattacatg tcgagaccga aacttaatcg
    13621 gcttgcagtc ccatttactt ggattatcgc tcattggcgt taacgaaata ttagccataa
    13681 ctggtgatcc ttcaaaagtt ggtcacttac caggtgcaac caatgtctat gatgttaatt
    13741 ctaaaggatt aactgaactc gctctaagat ttaatcaagg tattaacact gacggtgatg
    13801 cgctgaagaa acgtacacac ttcaacatcg ctggcgcctt taaccctaat gttcgtaaat
    13861 tatatggtgc cgtcaaaaga ttagagaaaa agatagaaag cggaatgtct tattttataa
    13921 cacaacccgt gtacagcaaa gagaaaatca ttgaaattta ccatgccact aagcacttga
    13981 acaaaccatt tttcataggc attatgccta tcgcaagtta caaaaacgca ctctttttgc
    14041 ataatgaagt gccaggaatc aagatgtcag atgaaatttt acaacaattt gaagcagtta
    14101 aagatgataa agccaaaaca cgagaactaa gtcttaagct ttcaaaggat ttaatcgata
    14161 ctgttcatga atattttaat ggtttataca ttatcacacc gtttcaaaat gtcgaagatt
    14221 cattagaact tgcagcatac tcaaaatcta ttactgctca caaggaggca atattatgac
    14281 aacaattaaa acatcaaact taggattccc aagattaggt agaaaaagag aatggaaaaa
    14341 agccatcgaa agttattggg caaagaagat ttctaaagaa gaattagatc aaacattaac
    14401 tgatctacat aaagaaaatt tattattaca aaagtactat cacttagata gcatcccagt
    14461 tggtgatttc tctttatatg accatatatt agatacgtca ttattattca acatcatccc
    14521 tgaacgtttc caaggaagaa ctattgatga tgatttatta tttgatattg ctcgcggtaa
    14581 taaagaccat gttgcaagtg cacttataaa atggtttaac actaactatc attacatcgt
    14641 acctgaatgg gacaatgttg agcctaaagt gagtcgcaat gtattattag atcgctttaa
    14701 atatgcccaa tctttaaacg ttaacgctca ccctgtcatt gttggtccga ttacttttgt
    14761 taaattatca aaaggcggtc accaaacttt tgaagaaaaa gttaaaacgt tattaccact
    14821 ttataaagaa gtgtttgaat cattaattga tgcaggtgct gagtacattc aagttgatga
    14881 gccaatctta gttacagacg acagcgaaag ctatgaaaat attacacgtg aagcttatga
    14941 ctatttcgaa aaagctggtg ttgctaaaaa attagtcatt caaacatact ttgaacgtgc
    15001 acatcttaaa ttcttaagtt ctttacctgt tggtggtata ggtttagact ttgtccatga
    15061 taatggctat aaccttaaac aaattgaagc tggagatttt gataaatcaa aaacattata
    15121 cgctggaatt attgatggtc gtaatgtatg ggcaagtgac attgaagcta aaaaagtctt
    15181 aatcgataaa ttgttagcac acactaatga acttgtcatt caaccatcat cttcattatt
    15241 acatgttcca gtatctttag atgatgaaac attagataca agtgttggcg aaggcttaag
    15301 ctttgcaact gaaaaattag acgaattaga tgcattgcgt cgcctattta atcaaaacga
    15361 tagtgttaaa tatgataaat taaaagcacg ttatgagcgt ttccaaaatc aatcattcaa
    15421 aaacttagat tatgatttcg aaagcgtgcg tacttcaaga caatcaccat tcgcgcaacg
    15481 tattgaacaa caacaaaaac gtttgaactt accggattta ccaacaacaa ctattggatc
    15541 attcccacaa agccgagaag ttcgaaaata ccgtgcagat tggaagaaca aacgcattac
    15601 agacgaagca tatgaaacat tcttaaaaaa tgaaattgct cgatggatta aaattcaaga
    15661 agacattggc ttagatgtat tagttcacgg tgaatttgaa cgtaatgaca tggttgaatt
    15721 cttcggagaa aaattacaag gtttcttagt aactaaattc ggttgggtgc aatcatatgg
    15781 ttcacgtgcc gtaaaaccac caatcattta tggtgatgta aaatggacag cgcctttaac
    15841 tgttgatgaa acagtttatg cacaaagctt aacagataaa ccagttaaag gtatgttaac
    15901 tggacctgta acaattctaa actggtcatt tgaacgtgtt gatttaccac gtaaagtcgt
    15961 tcaagatcaa attgctttag caatcaacga agaagtatta gcacttgaag ctgcaggaat
    16021 caaagttatc caagttgacg aacctgcatt acgtgaaggc ttaccattac gctctgaata
    16081 tcacgaacaa tatcttaaag atgctgtttt atcatttaaa cttgcaacgt cttcagttcg
    16141 tgatgaaact caaatccata cacatatgtg ttattctcaa ttcggtcaaa tcattcatgc
    16201 tattcatgac ttagatgctg atgttatttc aattgaaaca tctcgtagcc atggtgattt
    16261 aattaaagac tttgaagata ttaattatga tttaggtatt ggtctaggtg tatatgatat
    16321 tcatagtcca cgtattccaa caaaagaaga aattactaca gcaatcaatc gttcactaca
    16381 acaaatcgac cgctcattat tctgggtaaa ccctgactgt ggtttaaaaa cgcgaaaaga
    16441 agaagaagtt aaagatgcat tgactgtgct tgtgaatgct gttaaagcta aacgccaaga
    16501 ataatcgcag catttatcat ataataaact aaaaacgggt gattttatga cacaatatcc
    16561 tttatggcaa caattaaata ctttaaaaca agcaacttgg gttgatttaa cgcatacatt
    16621 tgatccggaa ataccacgtt ttagtgagtt tgaaaaaggc gaagtttcaa ctttattcac
    16681 ggttaaagat catggtttct atgtgcaacg ttggaatatt gtaacgcaat atggtactca
    16741 cattgatgca ccaattcact ttgtcgagaa taaacgttat ttagaagaca ttgatttaaa
    16801 agaattggta ttgccgttaa ttgtattaga cttttcaaca gaagtagcga acaacaatga
    16861 tttcatcgta acacgtgcgc atatagaagc atgggaaaaa gagcatggta ctattgaacc
    16921 tggcactttt gttgcacttc gtaccgattg gtcaaaacgt tggcctaata ttgaaaagtt
    16981 tgaaaacaaa gatgcgaacg gacaacaaca tgcaccgggt tggggattag atgctttgaa
    17041 gtacttaatt gaagaacgtc gtgttgaagc tgttggtcat gaaacattcg ataccgatgc
    17101 atctgtggac gttgtaaaaa atggtgattt agttggcgaa cgttatattc ttggtcagga
    17161 taaataccaa gtcgagttat taactaattt agatcaatta ccaactcgtg gcgccatcat
    17221 ttacgctatc agccctaaac caaaagatgc acctgggttc cctgttagag catttgcaat
    17281 taaaccatca aatgactaat attacccaat aaatccataa acaaataaaa acaggttatg
    17341 ccaacatgta attaatactg ttgtgataac ctgttttctt attatttata ctttgataca
    17401 actgcagcga tagcttgacc gccaccaata cacaatgatg ttaaaccagt ttcaacttca
    17461 tcattcagtt gatgcaataa tgtcactaat actctagcac cagatgcacc aataggatgt
    17521 cctaatgcaa tagcgccacc tttaacattc accttttcag gaggtaattt taattcacga
    17581 tcaacagcta atgattgtgc tgcaaatgct tcatttaatt caaatacatc aatatcgctt
    17641 aattcttttt tactacgttt caaagccttt tctacagcgc caactggtgc aatacccata
    17701 atagaaggat ctacaccatg acttccaaag ccatcaagca ctgccaatgg ttcgatattt
    17761 aattctttag ctttgtcttc tgacatgact aacatcatcg cagcaccatc attgattcct
    17821 gatgcattac ctgctgtaac tgtaccgtct tttttgaaag ctggtcttaa tcgacttaat
    17881 ttttcgactg atacattttc acgtacacct tcatccttag tgactacgat tggttcacct
    17941 ttacgttgag gaatcgatac tggaactatt tcactatcaa attcaccatt ttgctgtgca
    18001 cgtactgctt tttgttgtga gtttacagca aatgtatctt gttcttctct tgaaatacca
    18061 tattgctcta ctaaattttc agcagtaata cccatatgat attgattaaa tacatctgtt
    18121 aaaccatcat ataccatgct atcaaccatt gattgatgtc ccattttaaa accaaagcga
    18181 ctgttgttga caagcattgg tgattgagac atattctcca taccgccagc tagcacgatg
    18241 tcattttcac cagtcacaat agattgatat gctaattgaa tcgactttaa cccagaacca
    18301 catactttat tcaccgtaaa tgcaggtact gtttctggca agccaccttt catagcagca
    18361 attcgtgctg gattttgtcc ttgtcctgct tgtagtacgt taccgatgat aacttcatca
    18421 atctcacttg gattcaaacc cgtctcttta ataatatgtt ctattaaagt cgcacctaaa
    18481 tcataggctg gcacgtcttt aaacgcacct ccaaaaacgc caataggtgt cctgtatgct
    18541 gctgctaata cgactctcgt catgtcatat tcatctcctt atgatatata caattttatt
    18601 actaatacgt ctaatgaacc aatacgccac taacatccaa tttatgaaac caattgctac
    18661 gtatgttaca ttttcattct tgttatgcat ttaacaaatc atattataca atgatgtaat
    18721 ttgtttgggc aatcggaaac gtaaacggtt tcactttaac atttcaccct cttaaatatg
    18781 ctctatcatc ctcatctata gtacaaaatt atgctttgat ttaccacaaa ttatcattaa
    18841 tcatttcgcg taatgactct aattaaattc tatctatcat tgcatcgcta ttcacagctg
    18901 gtttcaccat caatttctca tcaatttttt attaccatga cgtctccagt caaatattaa
    18961 atcgctgatg attgttaatt ccgcaattac cttgcagtca cctctccttc cacttcatcg
    19021 ccaaatctgc ccctattcac tagaaaaaat aaatagtata cttagtaaat taattacaca
    19081 tcttaaagga gttaaaattt tatggttaat gcattaacac caaaacattt agctgaaaag
    19141 cgtgcaggat tccatgagtt gttctttgat ttaattttcg tctatgccat tcaaaaaatt
    19201 gcacatgtga ttttaacaac acaaaacggt tccatttcag ctgatttatt tttcaaatac
    19261 atcgtcatga gtttgttctt atggttaatg tggtctcatc aaacattttt tacaaatcga
    19321 tttggtcaag ttacattcaa agacgtctcg tttatgatgt ttaatatgtt catcatggtc
    19381 ttcctatcta acagcctata tccggacttt gaaaagacat tcttcccatt cttcttatgc
    19441 gtggcaatta tgtatttaag tatcggatta cagtatttat tacatatacg tacagggtta
    19501 gattatggcg ataagcgtac atgtcaagct tttgcgagcg tagcctttgt gatttcattt
    19561 ttatcatttc tatcattagt actaccacaa agtatccatt acatccctgg atttttaggc
    19621 gtattcattg cagcgacagg acttattcca tttcagaaat atcttgtatt atcacctgtt
    19681 aatatgatgc atttagtaga acgtttttcg ttactaacaa ttattatctt tggcgaggta
    19741 ctggttggtt tagcttcgtc atcatttagc attgatcatt ttagttacat ttatatattc
    19801 caatttatga ttttaattag tcttttcggt gtatattgga tcattactga aaattatatt
    19861 aatcataaat taacatctat cggtttccgt ttatcgtata cgcatctatt aatcaatatc
    19921 gctttaggtg tgattaatgc tgcaattgtt tttagtaata acaataaact aaatgacttg
    19981 tttgaaatta atatgatgta tatcagtgtt ttaatattct atattggatt atggttaatc
    20041 acgccttatt tccataatga actgacaaac gcgaagtata tttcatattc actaggtata
    20101 ctcgttatgt cttatattgt cagcctttta tttaaaggtc acgaccatgt aatgattatc
    20161 tcagtatcag ttgcaacctt ctgcatcatg ctgatttatt ttaaaaatca acgtctacgt
    20221 aataaacatg aataaatagt atttacctcg gtcaaagtat tggaactgaa tccaaaagtg
    20281 tcgaacttca cattatactt ttgtgacaaa cttcagttat gctacatgat cgaggttttt
    20341 aaatatttcc gaggaaatcg ttagcctata caatcatcaa atgatactta taaataacat
    20401 atatcctcta atccccacta tctaaaagga aataattccc acacaacata accacctcaa
    20461 aacaaataaa tcaatataga tatgtcacct atacttatcc cccacatttc attccacacc
    20521 aaaaagaaca gatgacaatc gtaaagattt atcacctgct ctaaatatta tttcaaatac
    20581 tatcatcacc aaaatcgaca attatagttc ttgtttgtta aaaattttaa tagacaatat
    20641 ataagaaacg ctcgttataa ctataccaat gaccgcatat gctatgacaa ctagccatcc
    20701 ttcagtgcta attttttcaa attctaatgc agatgcacca ctaaccatac catatataaa
    20761 gaaaaagacc acgaaagaaa taagtagtag tatagatgca catatcataa tgacattgga
    20821 gttttcagcg ccaaatttaa atgtcaatgg gaaaatgata gagtatgccc ctatgatacc
    20881 aaaactcatt aaacctgaca taataccaat catcacactt tgggtcacaa tcgtagtcac
    20941 aactaatcca atcattaaac ttgcaccgaa taagattaaa taaaaggcaa agtatgactt
    21001 aatataatca ctacgtttaa ccggtaaagt agatacataa tacatccatc ttgagtcttt
    21061 ttcatgttta atattatcag taataggtgt gattaacata accccagcca ttgccgaact
    21121 catcaacgga ttaaatactg caaagtatcc tgcagctata atagcgacta taaaataaat
    21181 atatgtttgc tttctcgttg cataaaaact acttaggaac atacctttca ttatacttca
    21241 cctcgcatta tgatttttgt agcgtcatca atgtgtttta aaggctctgc attttcaatt
    21301 tctgcataat ctttaactaa aatttgatat ttcccctttg acaatctaga agcaatgatt
    21361 aaatgcttag gaatttcaac atctttatct tctgtcgtaa caattccata ttgtgctaaa
    21421 agtatatctt tctgttcagt taaaatcatt cgtccatctt tcataaacac taatttatca
    21481 gctaaatgtt ctatatcttc agaaatatgc gatgatatta agatgcctcc accttgtgcg
    21541 acaaaatctt ctagtatttc cattacttct tctcttccag aaacatccat acctgcagtt
    21601 gcttcatcta agattaataa cttcacatca tgagaaagcg caatcgttaa agctatcttc
    21661 attcgcatcc ctcttgaaaa agttttaatt ttagtttgta gtggtaactc gaaatatttg
    21721 attaaatcaa agaatttttg actattccaa gtcatataaa tagattgaaa tactttatca
    21781 atatctttaa tagtcaattt attaggcact ctcaaatcat caaacactac acctatgtgt
    21841 tctttatatt cgacatcatc cacggtatgt tcttctccaa aaaatgtgat actaccgtta
    21901 tctttatgtc gattgcctac tagaatatta attaatgtcg atttacctga gccatttttc
    21961 ccaattaatc caactgtctc attagtagaa atcgttaaag atgcatcttg caatttaaaa
    22021 tccgaatctt tgtattgttt gttcacacta tctacgttta acaatgttgt catatccatg
    22081 ctcctctttg tttaatttta ataaaaacgt cttctcttca ataagtaact aaaggcgtta
    22141 tatatgaaaa tagcaatgat aagtagcaaa ctaaagcttt gattaattcc agtagtaatt
    22201 gaataaagcc caattactac aatggctagt atcaatattg ataagttgat cgcatatgtt
    22261 ttaaataatg caattaattc tatatggcgt tcaccttcat ccaatatttc caatcgcttt
    22321 tcagtgtaat tcttatctgc aatttttggt attctgtcat caaaccttct attaaacagt
    22381 gtaaattgtg tattgaaaat agcactagca aaaaatggta ttaaaaagaa tagtattgca
    22441 tttgctgcag cgtgccctac cacaaaaata agcaatacta aaaaagcaat aactgtctgc
    22501 agaatactta atatacttcc atttaaaaca taacgattcg caagcaactc atattgatct
    22561 gttgcatcga tatctacctc tttatctaca agttgcttat acttcaatgc acgtctttga
    22621 ttcatcaaaa caatggcttc taccaatatg ataataatcg tcgcaacaat cgatattaca
    22681 acgacattgt tatatgtcgc aaacgtcaag ttctcaatac caaatccatc aaaattacct
    22741 aaaattccac ctatgatacc ccctacaagt ccacccagta aaagatatcc gatatatctt
    22801 agtattttca tgcctcatcc tcctcaataa taaaaacagt ttccaccgtt tcattgaaaa
    22861 tgcgagcaat ttttattgcc gttaatactg atggcataaa attgtttcgc tcaattagcg
    22921 atatggtttg tcttgaaacg cccgcttgtt tagcaagttg cgtttggttt aagccatctc
    22981 gtgctcgtaa ttctttcaat cgattacgca catcgcatca actccttaat tacactgtaa
    23041 atgatatgcg tctttttgac aactatattt gtcaaatcta caccaaaaaa tatgattatc
    23101 cacctatgta tgacattttg aaacaaacac ctcaacgcct acaagtcata attgtttact
    23161 ttcgttacac cttcctgcat aattaacagc attctaattt tagtatgatg cacgcatttt
    23221 cactaaatca aaccattcaa aggagactat tatggcattt acattatctg caattcaaca
    23281 agcacatcaa caatttactg gtgttgactt tccaaaacta ttcaaagctt ttaaagatat
    23341 ggggatgact tacaatatcg tcaacattca agatggcact gcaacatacg tacatcaatc
    23401 agaagatgat atcgttacgt catctgtaaa aagtaatcat cctgttgctc aaaaatcaaa
    23461 caaaacaata gttcaagacg tcttaactag acatcaacaa gggcaaacag attttgaaac
    23521 attttgtgat gaaatggctg aagctggcat ttataaatgg catatcgata ttcaagcggg
    23581 cacttgtact tatatcgact tgcaagacca agctgttatt tcagaattaa tccctcaata
    23641 aactatattt atagcaacat tttaattatt tcataaaatt ttattgataa tcattatcgt
    23701 tcggtataaa gtaaatacta tatactactt atgagtgagg ttgattatca tgataactaa
    23761 cacttttatt ttaggcatca caggcccaac aagtcttgtc gtcattagca ttatcgcttt
    23821 aattattttt ggtccgaaaa aattaccaca atttggccgt gccatcggtt ctactttaaa
    23881 agaatttaaa tctgcaacag aagatttaga taaagagtct cacgatacac ccagtaagga
    23941 atcgaaacaa cagcgagagc aatagcactg accacacctt actggttcac tttagcgaac
    24001 tacgccatcg gttagtaaaa attttattgt cgttcgtcat tacggtcatc gtcgtatatg
    24061 tttcatcatt ttggtggatg acaccattca taacgtatat tacccgtgca catgtgtcct
    24121 tacatgcatt ttcattcaca gaaatgatac aaatatacgt gatgattata tttttcatcg
    24181 cgttttgttt catttcacca gtcatgtttt atcaattgtg ggcattcatt gcacctggat
    24241 tacataataa tgaacgtcaa tttatttata aatatagttt tttcagcgtg ttattattct
    24301 gtgctggcgt cgcttttgca ttttatgttg gctttccaat gatcattcaa ttcgcgttaa
    24361 aattatcatt aactttaaac atttcgcctg tcattggctt taaagcatat ttgattgaac
    24421 tcatacgttg gttattcaca tttggtcttc tatttcaatt accaatatta tttatgggtc
    24481 ttgcaaaatt cggtcttata gataccacat cattgaaaca ttatcgtaaa tatatttatt
    24541 tcgcttgctt tgtattagca agtatcattg caccacccga tttaacatta aatatattac
    24601 tcacattgcc actcatattg ctctttgagt tcagtatgtt catcgttaag ttcacatgcc
    24661 gaggcaaacc gccaacacat taaccttata cgaatcaatg ctgtgattat cgttatactg
    24721 caataccgaa tatttccaat atagcaacaa aaaaacaggc actcggcctg ttttttattt
    24781 tttaaattta aagattaaga tagcaactac cataatataa gcaccttgtg ctatcaatgg
    24841 ttcataactt ggataaaagc ccaaccagtt aatcgttggg aatccttcaa taacatgtct
    24901 tggcatcgca cctaataatt gtaacttttg aatacttacg ccaagcattt tgaatcccat
    24961 aataaaaata aagatcgaca acactctaaa tatatagaaa ataggtatta atttaactat
    25021 aaatctaaat aataatgcaa agatgattaa aataacgata gctaaagcaa taccaataat
    25081 aaaatctttg gtcgctagct cacctatcat ccccatatag aaaatgataa cctcgacacc
    25141 ttcacgcaac acagatatta aaccaatcgt cgctaacaat accaaattac cattactaat
    25201 cgcattagca tacatatttt taatcatgtc attccaacgt tttgcatttg aacgtttgtg
    25261 catccaaaca ccaacgataa acattaatat gaccgcaacg atacctaatc ccgcttccat
    25321 actttcacga agaatgccac tattccctaa agtttctaca aacgtaattg ctaagataat
    25381 actcagtaca agtccggcaa ttgcaccacc aatcacactt gcagtccctt tcttatcttt
    25441 tacattacgc gtcatggtag tcaatgtcat tacaattaac aacacttcta gcccttcacg
    25501 taaaaagata atcatcacat cgacgaagct ataactatgg ccaacaacct ctttaatttg
    25561 gttatttaaa tctactaaac catctttcac atgtgcttta ttatgttcgt ctaatacact
    25621 ttgataatat ggtattttat cttcaatttt cgtatacaaa gcaccgtctt tagtttgaat
    25681 ttgaccttca acatacggcc aagtttctat aaaatgtgta agcgcagcat cagcatccga
    25741 caattgattg tcgtcgatag ctttaatcgc cttctctaac gcatcattta attgtgatac
    25801 atggtattga tcatttgcag acgtattact ttttttatcg acatgatcaa tatttgattt
    25861 aaaagttgtc caagcatgtg acacttttgc cgtatctaat ggtgacttat gaattgcaat
    25921 tctaagttgt aataatgcga cttcaatttg tccatattga tttgcgtcat aattgcgaat
    25981 cactgtttca ttacttgtcc aaatctgatt caaactattg ttcaaagatt ctaattccgc
    26041 tttattttta tctttaatcg cttttgtcat cgcagcatct ttagcatcga cttgctgttg
    26101 caatagttta attttagaac ccgcatcttt actagccaat ttctcttcat aagcaattaa
    26161 tgacttcgtt aattgcgaaa gtgtatcttt ttgattatca ttcgcttttg catcttcaag
    26221 ctttctcaca tctgatttga cagcattact ttcactatta tcttcaagcg ataatttctt
    26281 aactgcactt accacttgct caattgcttt ctgcttattg tcattcgata tcgaattatt
    26341 agaaagtgca gatttcgcat ccgttatcac actatataca tcactaatac tttgttgttc
    26401 tgctgcctga cttttcagta acccaaagct acacaccata gcagcagtta ttagcattgc
    26461 tacaaattta gtcaaataat gtttcaccaa ggtatcctcc cttactaaca cctggtaata
    26521 ctaaaaatga agcagaacct ctatgtgtaa tatattcatt taatttatca ttactaccta
    26581 aattattttg tatatcgata aattgttttg tcgctttttg aaaagcaata aaaagtaagc
    26641 ctgtttcgaa gttacctgtg cggtcatccg taccatccac ataattaaag gctctacgta
    26701 aaattgacgt atttgcttct ttcgctagcc tcgtatgggc atctttatca ataatatact
    26761 cgccatgact atctttcgct tttaagtcaa tttcatcaaa ctctttccca cctgttaacg
    26821 gtgcaccact atgtcgtttc cgaccaaatg tagcctcttg ttcttccagc gcagtacgat
    26881 cccacgtttc aatgtgtatt tgaatacgtc tgacaacgca ataagttcca tgtttcgccc
    26941 atccgtcatc aataaacaca taatctttaa gttgattatt cttcctagga ttaattgttc
    27001 catctttaaa tgccattaaa tttctaggtg tttccttacc tttagcagag ataaaaccag
    27061 attgcgccca acgtacctta acaatatctc gaaatggacg aactaaatta tgaaccgcat
    27121 gaaaggatac ttgcgaatca tttgagcatg cttgaatcat aatatcacca tcgctgtaat
    27181 cgtctattaa ctgatcattc ggaaaatgcg gtaaatcttt aaaggcatcg ggaattttac
    27241 tagataaccc aattttcttc atcaaagact tactaatccc aaaggtaatc gttaacttgc
    27301 ttgcacctaa tcctatcgat tcaccggtat ctactggtgg cattaaagga ttgttactcg
    27361 gcttgccaac tgtatcacca tccatcatac gatcagccat aaccgtccac tttttaaaca
    27421 ttgccttaat tgcatctcta tcttttgact tcaaatctaa cgcaacgaaa ttgcatgttt
    27481 tttgcgtggg tgtggtaatg cctggttgca ctttaccata aaattcatac gcatcttttt
    27541 ccggatcttc tggtgtattg aacattgatt tgaaagacca catgctacca acaccacttg
    27601 cgccaattgc aacaccggca ccgccaatac ctaacatttt caaaaatgta cgtcttgaaa
    27661 attgcgtact atcgttaact tgttcataat ttgtcattca gtaatcacag ccattttact
    27721 tagtggctct cctaatgcat taacagcatc cgcaaatgct ttacggtctt tcttcgatac
    27781 tttttcaaat gactcataac cgccgttgtt atctttatat ttatccaata attgattcac
    27841 tttatcgaag ttcatttgga tatcatcact taatttttta tcttttttct ctaaaatagg
    27901 tttaaataag tcataaattt tttgtgcgcc ttcaacgttc gctttaaaat catataaatc
    27961 tgtatgtgaa taaatttctt cttcacctgt gattttagaa gttgcaactt catttaatag
    28021 gtcaacagaa ccttgtaaca ttaattttgg tatgatatct aatgtatcag ctttggcatg
    28081 caattctttt gcatctttca ataattgttg tgcatctttt ttagtcacat catcaatttt
    28141 cttatcttcg tataatgctt tttcaatctt atgatatcct gaccattctt tttccttttt
    28201 ctcttctttc atatctgcaa gacgtgcatc aattttagga tctaaatctc caaatgcttc
    28261 tgcaactggt tcagagcgtt cataatacat acgaactttt ggatataacg cttttgcttg
    28321 ggccatatca ttattttcaa tcgctttaac aaatttttct gtacctttta aaaattcatt
    28381 taactgttca tcagtatatt tatcatatgc ttttgttgct tgttttaatt ctgctttaac
    28441 accatcatct tttttcgatg tctttgaatc atccttctta ctatcgtcgt tcccacatgc
    28501 agcaattaat aacgttgatg ctaataatag cgttgttaac tttttcattt tgacacctca
    28561 ttatagaaat tctattatcc ccatagatgt gttcttataa atattatttt cgcattccct
    28621 acacgctaaa ataatacatt acacttaaaa cggctgtttt aaagcatcct cccataaaca
    28681 tcatctagtt gataataatt atcaaaatca ttatattgtt ataaaataat actgtcaaca
    28741 accttttatt tttaaaaatt aaataaaaga gcgctgccat tttaaatgtc atcactcttt
    28801 tgtaagtcta atttgtcatt tttggtcgta ttctgatttt aataaactat agatgtaact
    28861 cgatgaataa ataccattta gtaattcatt gtcacgtaac ataccttctc tagtaaatcc
    28921 cagcctttca ggaatagccc ggcttttttc attattaact gcggcactaa tctcaattcg
    28981 gtttaagtca agctcttcga aacaatactt tatcaatgcc tcaactgctt gtgtcataat
    29041 cccatgaccc tcaaattctt tatctaaata gtaccctaat gaagtttttc tgtgcatgtg
    29101 attaatttca tgtaaaccga tgacaccaac tagcgttcct tcataccaaa tgccacactg
    29161 aaatccatta ccatcagcaa attgcaaaag tcctctttta ataaatgcac gcgtatctga
    29221 tggttgctca gttgcatcta cccaaggtaa ccattcccta agtgaatttc ttgaacgatt
    29281 gactaaattg aaaagcgctt ctgtgtcatg agcttctaaa atttttaatg ttatttgttc
    29341 attcactttc attccaaaca taccatcaca tcctcattca tttttcatat aattctgtaa
    29401 attatcatta taataacata ttaatgttac gcattgtatc ttaaatttgt tgtccttccc
    29461 caacttgcat tgcttgtaga atttcttttc gaaattctct atgttggggc cccgccaact
    29521 tgcattgctt gtagaatttc ttttcgaaat tctctatgtt ggggccccgc caacttgcat
    29581 tgtctgtaga aattacggac ccaatttctc tatgttgggg cccatcccca acttgcacat
    29641 tattgtaagc tgacttttcg tcagcttctt tgttggggcc ccgccaataa gcgttgtagt
    29701 gcctattact ttgattgatg tccaagtttc ctatcacaca acttaattta atcactactt
    29761 tattattcat tccaaattta tatttaaaaa tacgacaaaa cagtgctaaa atgctattaa
    29821 aggaggcact gctcatggca tcattgaaaa ataagcattt tatgattggg ctatcactaa
    29881 catttatagt cgcattattt agctttttag cagctaaatt gccaatccta gataaggttg
    29941 gcgcattaac tatcgcgata ttaatcgcta ttctatatcg tcactttaga ggatatcctg
    30001 aacaatatag ctcaggtatt acgttttcat ctaaatattt attaagattt gcaatcatcc
    30061 tatatggact taaactcaat atattcgata ttatcggtca aggtagtaaa ctattagcca
    30121 tcgatgtagg tgtcgttatt ttcagtattg taatgatgct ctttgtcaac aaactgttgc
    30181 atggtgacaa aaacatcgca ttattactag gtgtcggcac aggcgtttgt ggtgctgctg
    30241 ctattgccgc agtcgctcca atattcaaat cacgtgaaaa ggatacagct attagtatcg
    30301 gtatcatcgc attgattggt acgatatttt cacttatata tacagctatc tacgctatct
    30361 tttcaatgac gacaaatgtt tatggcgctt ggtctggtgt tagtcttcat gaaattgcac
    30421 acgttgtctt agctggtggc tttggtggta gtgatgcact taaaattgca cttcttggta
    30481 aacttggtag agtattctta ctgattccat taaccatcgt acttatttta attatgcgtt
    30541 tccgttcatc agaatcatct agcaagggtc gaataagcat tccatacttt ttaattggat
    30601 ttgtcattat ggccttagtg aatacatatg taactattcc gtcagcattg ctaaatattt
    30661 taaatactgt atcaacaatt tgcttattaa tggccatggt tgctttaggg ttaaatgttg
    30721 catttaaaga tcttaaaaat cgcgcactta aaccgcttat gacaatcatt attacctcaa
    30781 tatgcttgtc atcacttgcc tttatcgtcg ttcattggct atatagttaa tatgaaaaga
    30841 cgctcaaaca cttacaaatt tagtgtgccg agcgtcttat ttttatactt taaaagtcgt
    30901 attggttgtt ttcttcttca aacacttgtt gttgtacttt ggcagttcgc attgtggatt
    30961 gtgccaacgc ttttaaccga aatacaacat catcattgac aatttgttga tttgaaaaat
    31021 ctttctcttc aataaataca tacgtttgca tcgtatgtgc tttcatgtaa ctcaatattg
    31081 gttttaaatg catttcagga attaaataat gtttactaga acctgctgtc gctacaagtc
    31141 ctattacctt gtcacgaaac gcattgactg gaagtagatc aaacacattt ttcaaagcac
    31201 cagggatgga agcttgaaaa attggaaaac caataaaaat cacatcagcc tgcattaacg
    31261 acgtcgttaa tttatataca tctcctgtag tatctagata attctttcca acgctaaatt
    31321 ctaattcaag ttctcgtaaa tccatcaact ctatttgatg tccctcattg atgacttcta
    31381 gttcattttt taaatcatcc atagcaattc tcgttttaga acctactgtg gaacctgaca
    31441 ataatacaat attcatgatg acgcccctcc tattttgata aatgcttttt aatcgccggt
    31501 ataatgtcgt tgccaattaa ctcaatattc ttcataacat tttcaaatgg cacaccgcca
    31561 aaatcaagct gtgccataaa acgttgatga ccatacaact cgtgttgata caatatcttt
    31621 tcaataattt gttgcgggct tccaaccatt agcgcttctc gataatctga cgaattagca
    31681 aattgctgtt tcggataacc aacaccacga ataaatgaca tccctgtatt caaatgtgga
    31741 taaaattctc tcatagcatc ctgagttgtt tcagctgtat aaaacagact cgctgtactt
    31801 actggtaaag acttaggcga agcatcgaaa cctgcttcag ttgccgcttg acgataagca
    31861 tctatagaac ctttaaagtt cattgctggg ccaccaaggg ttgtaatcat cattggcaca
    31921 ccttgttttc ccgctttaat agcacttgca ggtggaccac caacagcacg ccatattggc
    31981 aatatattat cgattggtct tgggaatatt ttcatatttc taagttctgg acgatatttt
    32041 ccagaccaag taatacgctc agttttattt aactctaaaa gtaaacctaa tttttcttca
    32101 aacaattcat catagtcttt taaatcatag ccaaacaagt caaaaatacc tgttcttgat
    32161 gctctgccag ctacaatttc ggctctacca tgagaaatca aatctaatgt cgcgaagtct
    32221 tcaaatactc ttacaggatc tgttgcacta ataatcgttg aagaacttga aactttaata
    32281 tgctttgtcg cttgggcaat tgcacctaac acaaccgtat gtgcctgtgt tgtaaaatgc
    32341 tcctgatgac tttcaccaac tgcaaaaaca tcaatacctg cttcatctgc taatttactt
    32401 gcttcaataa tttcattaat acgttgctca taactaactt tttcaccttt caatggattc
    32461 aataaatgat cacctaagga atacaaacca aactcaagag gcgtattttt attcatttct
    32521 aatttggcca ttaaacattc tcctttatat ttcgtgtaac ttactttcaa tttgttcacg
    32581 ttctgcttct aaaaagtcag gcaagttcaa ttgacttcct aattgttcaa caggtgtatc
    32641 aatagtaaat ccaggcgctt cagtcgcaaa ttcatacata attgaattat ggcgatagta
    32701 taatgattta aagaaatagc gatcaattat acctgaatta ctttgtggtt gttgttgtaa
    32761 tttcttgtaa attgcatcta agtcactcat ttgtggtgta ttaactgcaa tatgatggat
    32821 ataacctcgt ccaggtcttt cacgttgtcc ctgttgctca ataactacaa aatccgaata
    32881 taaaccttgt ggtgctaatg tcagcacatc gaatgattta ttatctcttt tgcgataacc
    32941 taagacattt tccaaaaatt ctaccgtacg cgctgcattt ctaactctta attcgactgg
    33001 ccctattcct aaaatttgat atgcttgagg tatttcacta taagcgttat gttgccattg
    33061 atgtggtact tcaaaactat cattaacaag caatatgatt tctaagtcgt cttcatcttt
    33121 aaagacaaca ccttcttgtc caagatagta caaacgttca gacttaatac cattattctc
    33181 aagacgtttt tcaaaataat gaagtgccgc ttgatttggt actaataatg aaaatcgata
    33241 aatcgtttca gtacctggct gcttatgacc cgcattggga atttcaaaaa agcttaaaat
    33301 tgttccggct gtacctactt cgtccccata aaacaaatga tacattgaag gattgtcttg
    33361 attaaccgac ttttcaacta atcgtaatcc aaggacattt gtgtaaaaat ccttattacg
    33421 ttttgcatct tttgtataca tggatatgtg atgatgccct actatattca tactatccct
    33481 cctattagta taaattttat accagcttac ataaaaaaga tagttatatg ccatttaccc
    33541 cttgcaaata aaaatatgtt tgtactcatt cgtagataca cagctagtat gtataacgac
    33601 ataataattg cataattata aatttatgtg tagcaattta ttttttctaa atgtttataa
    33661 aatacttcaa taactaaata tgcaagattt cacaaaagca tattgacaac gctttcatac
    33721 atgtctaata taagtttatc gcacaaaatg catataaata catattaagg aggattttat
    33781 gaattttctt aaacctgcaa agcatattaa gcctttgcca gaaaatcaga tagatgatac
    33841 ctataaacga ttacgtctcc aagtatttct tggtattttc atcggttatg ctgggtacta
    33901 tttattacgt aaaaactttt cgttagcgat gccggcattg caagagcaag gttttacaaa
    33961 agcggaacta ggttttgcac tttctgctgt ttccatcgca tatggattta gtaagttctt
    34021 tatgggtact gtaagtgatc ggagcaatgc tcggatattc ttagttcttg gattagcact
    34081 cactgctatc gtcaatttgt taatgggatt tgtaccgttc ttcacatcag gtatcggtat
    34141 tatgtttgtc ctattattct taaatggatg gtttcaaggt atgggctggc caccttcagg
    34201 ccgtgttctc gttcactggt ttagtgtaag tgaacgcgga agtaagactg ccctttggaa
    34261 cgttgcgcat aatgttggtg gaggtattat ggcacctatt gctgcttggg gtattacaac
    34321 aactgcattt atcaactttg gttatttaaa aggtttcgaa ggtgtattca tttaccctgc
    34381 actcttagca cttatcattg ccgcaatttc atacgtattg attagagaca cacctcaatc
    34441 tcaaggttta cctccaatcg aaatttataa aaatgacttt gctacaagcg ataagaaaac
    34501 attagaaaca gaattaacta caaaagaaat tttatttaaa tatgtactga acaataaatg
    34561 ggtatgggca attgcctttg caaatatatt tgtttatttc gtgcgttatg gtgtacttga
    34621 ttgggcgcca gtctacttaa gtgaagaaaa acatttcgac ttaaaagcat caggttgggc
    34681 atacttctta tacgaatggg ctggaattcc tggtacatta ttatgtggtt acatttctga
    34741 taaattattc aaaggtcgtc gtggacctgc aggtttcttc tttatgttag gtgtcacagt
    34801 atttgtatta atttattggt taaatcctcc aggcaatgct tggttagaca atgtctcatt
    34861 aattgccatt ggtttcttaa tatatggacc agttatgtta attggtttac aagcattaga
    34921 ttatgtacct aaaaaagcag ctggcacagc agctggatta acaggattat ttggttatct
    34981 gtttggtgct gtaatggcca acatcgtctt aggtgctgta gttgataaat tcggatggga
    35041 tgtcggtttt attttattaa cagcaattag tgtgtttgca atgttgagct ttatcctcac
    35101 ttggaataaa gtaggacaag aaaccgttca tcattaaatg ataaaaaata aagtcatatg
    35161 gttatcttat cgaaagatga tatattcatc tcttataagt tcaaccatat gactttttat
    35221 tagtattcaa aaaaatattt acattgccac ttttgtgttt gccctgctgt tttattcaat
    35281 tgattacacc acttaggata aactctaaaa gccattttcc cttgatactt agatgaagct
    35341 aagatacctt tttctaccaa taattcccta ggaaatagaa agtagccatt taattcatca
    35401 tcgataacag caacaatcag gtaatcagca aactcttcaa ttttgtatgg ccgattataa
    35461 ttatcttcgt cttttgtcca acatgtcacg aaatatcccg atttagtcgg tgttttctta
    35521 gctaacctac tttgatatgt ttcctcttta aagctaaatg ttaatgcttc gtaatcttga
    35581 ttatattttt cttcagttaa atctttaact tctgattctt cactaaaaat atttttcaac
    35641 agtattttag atttacacat atgccaatct cactttattt tttcataatc gtatcatata
    35701 tttatttttt tcgaaaaata cacttccgac cgaaatatac tcagccagaa gtggacgaaa
    35761 tatttaacct tctattaatt cagatgtatc aactgtcaaa cgatcacgta ataaatagac
    35821 gattaacatt gctgctaaag cacaaagtga ctcagcaatt aataatgacc aaatgacacc
    35881 tgttagtcca aacaaagcat tcataataaa taatactgga atgataattg caccttgtaa
    35941 aatggccata attgtagcac cacgaccttg cccagtcgct tgaagcatac cagtaaacaa
    36001 gaaacctata ccatttaata ataatgatgc cattgttact ttcaaaataa atgtcgccat
    36061 ctcaacaatg gcttgatcag tagtaaatag tccgaccata tgatgtccaa ttgtaaatac
    36121 agcactcata catacaacaa agataacgcc gatagacatg ataactgctt tgataacgtc
    36181 tttcatacgg cctttatttg ccataaagtt atatgcaatt agtggtacaa caccttcaca
    36241 taatcccatg ataataagtt ctggaaattg cacaagtcta aatgagatac cataacttgc
    36301 aatcgcgaag tttccataat gtgctaaaaa taaatttaaa actaatcctg tgaatcccat
    36361 taagatactc attaaaaatg caggaatacc gattttaaag atttcagaaa gcatttcttt
    36421 attaggtttc gcaagtttaa tattaactga cacaacgtca ctatttttca taaaataaat
    36481 gataaagaac agagcagcag caacattact gattgcagta cccaaagctg caccaacaac
    36541 gtttaaatca aaaccaaaaa ttaaaattgg atctaaaata atatttaagc ctacactagc
    36601 taacatacca atcatagaaa ccattggtgc cccaattgca cgtgcaaatt gttctaatat
    36661 gaagaacaaa attacaaaag gtgcacttaa aaacattact ttcaaataat tacttgttaa
    36721 agctaacgtt tcacctctcg cccctaaaat tgctgcgatt tgatcactga atggtaaagt
    36781 aactaaaatc acgataagtc ctagtgcaat accaccataa atagagaaac tacttacaaa
    36841 tttactctta ctatagtctt tcgcacctaa taaacgtgaa atataagttc ctgcaccaac
    36901 gccaaataaa ttacctaacc ccattaagat agcaaatact ggcagtgtta gagagatagc
    36961 agaaatcatg tggctatctt ctaaaaatcc tataaagtaa atatttaata tgccataaat
    37021 aacgcttaat aaagtcccta tcatcattgg caatgagaaa tgcatcatcg ctttaaatac
    37081 tggcgatttc tcaaaataat ataattgttc gtctttcatt gttcaatact ccttgtcttt
    37141 tccaataatt agcttactaa caattagata tctaactata atattaaaga caaagtgact
    37201 gatttctacc agtcacactt atcatttatt gtaaactaga taacatttta gttaagtttg
    37261 ctttcatttg ttcattttct tcttcagata actgcgatac gagtgtttgt tccatttcat
    37321 caaatatcga agtgaatgct tctacgagtt taatcccaga ggtagtcagc cctatattct
    37381 ttcttctcgt atcttgtgca tcgacatagc gatagatcag ctttttacgt tcaaggttcc
    37441 ttaataaatt actgacagtt ggacctgttc gttgtaatgc tttagcaata tcattttgtg
    37501 tcagtccatc ttgttgatgt gcataaagat aacctaacgt atgaccttgc tcatttgtaa
    37561 tatcaaattg ctctaacttt tgatcagcct tttgtttcat ctcatgacta atcattctaa
    37621 ataaatacga ataagtgaat tccatttatt gcaccaccat ttcgttagat gtctaactaa
    37681 atatactgcg acatttttaa attgacaaca tgaatatcta atttatttta aaagttccta
    37741 taaaatcttg cgccataaac attttgatat gcaatttttc ggattaaaat atgaaagaga
    37801 ttgcatatct acgtttttaa gaaatcgctt acaattacaa ataggaggat attacgttgt
    37861 taagttatag acaaatcgaa attttgtatc ttttaattca tgagcaaacg tttattccaa
    37921 tcaataccat tgctaatcaa cttggtgtat cacctcgtac cattcaatat gatatagcat
    37981 atattgagca atatgctgaa acttatcatt accaagtcag ccgtaataaa gctgcaggca
    38041 tcaaagtaac aacagctcac gcgacactat taaatgaatt agagcacaat ctaacaaatc
    38101 aaattcattt ttcaaaagat gaacgactca cacatatcgc tttaaagtta ttcgaaacaa
    38161 ccgatcctgt ttcaacaaag caacttgcgc aagatgttaa tgtttcgcgt cggacaattg
    38221 cagatgatat taaaatgatt caagcccaac tagatcaata ccatttaaag ttaaattatg
    38281 tccacaataa aggattcaat attattggtg aggaagatca ttatcgtaaa gcgtatgcac
    38341 actttataca tcaatatatg aaacaagcta caccttttat agaggcggat atctttaatt
    38401 cagaatcaat cgcattggtt cgccgtgcca ttattaagac attaaatagt gaaaattatc
    38461 atttagttca gtcggctatc gatggcttaa tctatcatat actcattgcc attcagcgtt
    38521 taaatgaaaa tttttcgttc gatataccta tcaatgaaat tgataaatgg cgacatacta
    38581 atcagtatgc tattgcttca aaaatgatag aaaacttaga acgcagttgt aatgtcacat
    38641 ttccagagtc tgaaattatc tttatcacgt tacatttact aggctctaaa atgactgaac
    38701 atacagcatc ttcaattacc tttgaatacc atgatttatc gcaaaatata catgaattga
    38761 tcacttgtgt tagccaagaa ttaggcattg atatgtcaaa agacaacaag ttacatacca
    38821 gtctgatcac acatatcaaa ccagctatac atcgtattaa atacgatatg ctacaaccta
    38881 atcctttgag gcaagaagtt atgcgtcgct atcctcaaat cattgaagcc gttagcaagc
    38941 atattagtcc aattgaacaa gatgctgcta ttcgcttcaa cgaagatgaa ttaacataca
    39001 ttacaattca cttcgcatca agtatagagc gtgttgcaac acataaacaa tcaatgatta
    39061 aggttgtctt actatgtggt tctggtatag gcacgtcaca acttttaaaa tcaaaactaa
    39121 atcacctgta tcctgagttt cacatttggg atgcctattc catttatcaa ttggaagaaa
    39181 gtcgattatt gcaagataac attgattatg tcatttcaac agtaccttgt gaaatatcag
    39241 ctgtaccagt tattcatgtc gatccattta tcaatcaaca atctcgtcaa aaattgaatc
    39301 aaattatcaa tgactcaaga gaacaacgag tcatgaaaat ggcaactgat ggcaagtcac
    39361 tcgcagattt attgcctgaa catcgcatca ttataaataa acaaccatta tcaattgaat
    39421 ccgcaattgc agtggctgtg caacctttaa tcaatgatgg cattgtctat tcaaattata
    39481 cagctgcaat tttaaaacaa tttgaacaat tcgggtcata tatggtcatt agtccacata
    39541 ttgcacttat tcacgctggt actgattatg tacagaatgg tgtaggtttc gcactaacat
    39601 atttcactga agggattatc tttggtagta aagctaacga tcccgttcac cttgtaatta
    39661 cattagcaac ggaccacccc aatgcacatt taaaggcatt gggacagtta agcgaatgct
    39721 taagcaacga cttatatcga caagatttct tagatgggaa tatttttaaa attaaacaac
    39781 acattgcttt aactatgaca aaggaggctt aataacgtgt cattagacat tttgtcaaca
    39841 acacgcatca ttgtaaaaga acaagtaaat gattggactg aagctataac tatagcttct
    39901 cagccattac tacaagaaca aattattgaa caaggctatg ttcaagcaat gattgatagc
    39961 gttaatgaac ttggacctta tatcgttatc gcacctgaaa ttgcaattgc acatgcaaga
    40021 ccgaacaatg acgtacatca agttggttta agtctattaa agttgaatca acatgtggca
    40081 ttttgtgatg aagatcacta cgcatctctc atttttgtat tgagtgccat cgacaatcat
    40141 tcacacttat ctgtattaca aaatttagca accgtactgg gcgataacca aacagtccag
    40201 caactattaa ctgcaacaaa tgcacaagac attaaaaaca ttttaaagga gcatgattaa
    40261 tatgaaaatt ttagtagtat gtggccacgg tttaggaagt agttttatgg tagaaatgaa
    40321 cgcacaagaa gcacttaggc aacttaatgc accatctgat atcgaagttg aacatagtga
    40381 cattatgaca gcaagtccag agatggctga cttgtttatt tgtggtagag atttagctga
    40441 aaatgccgaa cgtctagggg atgtcttagt tcttgataat attttagata aagctgaatt
    40501 acaacaaaag ctctcagaaa aattacaaca acttaacatg atttaaagga ggtacgacct
    40561 atgcaagcaa tccttaattt tatagtcgat attttaagtc aaccagccat tcttgttgca
    40621 ctgattgcct ttataggttt aatcgttcag aaaaaacctg ccgcaacgat cacttcagga
    40681 accattaaaa cgatattagg cttcttaatt ttaagtgcag gtgctgatgt cgtcgttcga
    40741 tctcttgaac cattcggcaa aatattccaa cacgcatttg gtgtgcaagg tatcgtacct
    40801 aacaacgaag ctatcgtctc actagcctta aaagattttg gaacaacagc tgcactcatc
    40861 atggtctgtg gcatgattgt taatatttta attgcccgct tcactaattt aaaatatatc
    40921 tttttaacag gtcatcatac attttacatg gctgcgtttt tagcaatcat tttaacagtc
    40981 agtcatatta aaggctggct aacgattgtt atcggcgcac tcgtattagg attaatcatg
    41041 gcagtattac ctgcattact ccaacctacg atgcgaaaaa ttacagggaa tgaccaagta
    41101 gctttaggtc attttggctc aatcagttac tttgccgcag gtgctgtagg tcaattattc
    41161 aaaggtaagt ctaaatcaac ggaagagatt aaatttccaa aaggcttaag tttcttacga
    41221 gaaagtacaa ttagtatctc gattacgatg gcattacttt acttcatcgc atgcttattt
    41281 gcgggcgtta gttatgtaca cgaatctatt agtgatggtc aaaactttat tgtcttttca
    41341 ttaattcaag gtgtgacatt tgctgctggt gtatttatta ttttaacggg cgttcgttta
    41401 atcttagctg aaatcgtccc agcatttaaa ggaatttctg aaaagcttgt accaaattct
    41461 aaacctgcat tagactgccc tattgtgttc ccttatgcac aaaatgcagt attaattgga
    41521 ttctttgtca gctttattac aggtgtcatc ggtatgttta tcttattctt atttggtggc
    41581 gtcgtcattt tacctggcgt agttgcacac ttcttcttag gtgcaacggc tgctgtattc
    41641 ggtaatgcaa gaggcggtat taaaggtgct attgctggcg ccgctctaaa tggtatccta
    41701 atcacgtttt taccattatt attcttgcca tttttaggcg aattaggtgg tgctgcaaca
    41761 acattctcag atacagactt tttagctgtc ggtatcgtgt tcggtaacgc agtaaaatat
    41821 atgggattat ttggtgcgat tctatttatt attatcgtag gtgcgacaac aattttatta
    41881 aaaggccgtc aaaaagaaca gcaatagtgt taacgtagaa atataaaaca ccgtcacata
    41941 ttgagtgaat gcccctttta tcaagaggaa agccacttac ttatggacgg tgttttgtat
    42001 tatattaaat gatacttagc catactatcg acagctgcta aaattgcttc ttcttgtgtc
    42061 gcaatcggtt cccaaccaag taatgttttt gcacgttcgt tacttacatt gcggttcata
    42121 tctaataaaa gtttaccttc tttagcttga tgattaaatt tagcacctag actcaaaata
    42181 aagtctggta attttttagt agaaactttt tgagctattt caggtctctt ttctttaatt
    42241 aattttgcaa tttccaacaa attaatttgt ccatcagccg tcgcaataaa tcgcttgcca
    42301 ttagcttgtt catttgtcat tgccaaaatg tgcagttcag ctacgtctct cacatcaaca
    42361 acatttaacg gaatttgcgg tacacgtttc attgaaccat tcaataaatt ttctaataaa
    42421 tgaaagcttc ctgaaacgtg tgcatctaat gatggcccaa aaattgcaac tggattgatt
    42481 gtggcaaatt ctactgttgt attttcattc tcaacaaaat cccacgctgc cttttcagct
    42541 aacaattttg atttttcata tactgataag cctggttcat cttcatttgt ccaatgactt
    42601 tcatttgtga ttgaattttt atctttatta ctaaaaccaa ctgcaccaaa gtttgcagtc
    42661 attaccacac gtttaacacc cgcatgttct gcagctctta aaatacgttg tataccttca
    42721 attgcaggct tcgccatcac ttctgcatcg tctgttttac cgaaaaacac cggagatgct
    42781 acactcaaga catacttgca atctttcatt gcttcatccc aatgttcatc ttgtgataaa
    42841 tccgcttcga caaacattaa tcgctctgtg gaaatgccat tgtcttgcat tgttttaatt
    42901 actttatcag ctttacttaa atcacatatc gtcgtttgta cgtcataacc ttgttctaat
    42961 aatcgtgaaa taattcgcat gccaacaaac cctgtaccac cggttgctaa tactttatta
    43021 ttcatcacat aatctcctct ttgtgtctat tgattactca ttatagcagt cattatattt
    43081 tgtatatatc aacagactta tattgcttaa tattctatat gaattgtaaa agtaaacatc
    43141 attatattcc ttaaaatatt aaactcatct attttcaaat acccaatcat ttagaaacaa
    43201 aaaccatata tatatcatac tttgaatagt taaaggcaat aatttccact catatgttca
    43261 accttcacca aacactattg tcaccttttc aagagcaagc aaacacttcc aatgattcag
    43321 catacataaa ataacaatat taacttaaaa tcatatccac taatgcttct accgtaacat
    43381 tgctaaaata ataaacgatg tctaattgct ttaactgatg cgttaaatct tctcttgtca
    43441 tttttgttcc ttgtaatgct tcttccacat cttttatatc accttgtcca aagaaatccc
    43501 cataaatacg acaatctgcg atacgatttt gttcaacaga aattgttatg tctaccgtac
    43561 ctgaagataa tctttcactt cgattgtatt catatttggg tgacttgcca taattccaat
    43621 cccaattttt atacttttta tcagctaatt catcaatcgc ttcccaatct gcatccgtta
    43681 attcatatcg tttcgcctct ttaatgtcat cgattcccaa aatctgcgtc accattaaat
    43741 ttttaaattc ttcaatggtt atatcacgat acttttcatc cagtgcttct ctaagatgac
    43801 caacacgtgc gcgcacagat ttaatccctt tcgatgcaat ctttttgcga ttaggcttta
    43861 acactttatc cattgcttca taattaacat caagtaatag cgaataaccg ccataaatac
    43921 gattattcat taatgtcatt gcggcgcctg acactttttt accattcaat gttaaatcat
    43981 ttctaccgct ttgtaccaca tctgttgcac ctaatgtatg caacgccttt atagctggtt
    44041 gataaaatcg ttgaaaatca ccataaattg aagtgtcttg ttctaaaata caacacatat
    44101 taacggcacc tttatcaaca tacacagcgc caccaccagt atctcggcga actacttgaa
    44161 tactgtgttt atctatataa tcttgattca cttctatagc agtattttga aaacgtccaa
    44221 tttccacttt tggatcacaa taataaggga ataaaatatc ttcatctaaa aagatatgct
    44281 ggttaacata aacttgcata gcgagtgcaa tcgcaccatc agtaatatat tctccatttc
    44341 taatcggttc tattaagtac atcttctgtc tctccaatgt tgttattttt cgttgtgctg
    44401 tcgttccgga gtgctgctgt aatcaatgtt gagatatcct cagttaaatg tatggtacgt
    44461 tcttgaattg aattcggaat gcgatatgcc tttttattca tcgtcatata aagggcattt
    44521 tcatttttac gtgtcatacg ctgaaaagga tgcttcacaa attgtggtgt agtataacca
    44581 attccaattt ccaaatacaa cactttatca tcttgatgtt gctctagaaa agcattataa
    44641 cgatgtagtt gcgcatgaaa ttcagcatct tcaaccatcc caacttccgc tttacgttta
    44701 ttcacttcca ttggggcatc acattttgga catcttggaa tcatctccca aggtataagc
    44761 atatcttgtt gcgcaacaac cattttacga attaaatcat cattgcgata cgtttgagca
    44821 tgacaatgct gactacactg ttgcagtata tactcccctt gtatatgaaa tacatgagtc
    44881 atatcataat cagctacatc gaaagcatta tctgcattcg tagttataat gtggtactgt
    44941 ttaccttcca ccaaggattt taaagcgagg taagactgac ctacaggttg atctaaatag
    45001 tttaatgtaa taaaacgact ctcaaatgcc caatactctt gccaactgcc ataaggatgt
    45061 aaactcgctt gcaacatatc aaagaagcga tatttttcaa taaaatctgg gaaattttcc
    45121 gtaaaacgct ctcctacata tgtaaatccg tcagatgcag acatgcctgc accaattcca
    45181 atcactatcg catctgcttc atcaatcgca gtacgcaata cttcagcctg ctttgtcttg
    45241 tcatccatta acagagacat tgcattccac ttactactct gcatcacggt taaatgcctc
    45301 cttatacagt tgtaaatcct tatctgtaaa tacattgaac acgactttca atgttgaatt
    45361 tgtttctttg agatagcttt ctactgttcg aacagcaatt tctgctgctt catcttgagg
    45421 aaaagcaaat acacctgtag atatacagca aaaagcgaca tgatttaaac tatgttgatc
    45481 agccaattta agacagctaa gataacattt agctaacaag tcctgattca tctttgaaac
    45541 aggcaatcga cgtatttgcg gaccaaccgt atgaattata tactttgctg acaaattata
    45601 tccacgtgtt attttggctt tacctacacc ttcattgcgc ccttgttgtc gaatgatctc
    45661 tgcacaatca agtcgaactt gaacacccgc ttttgtatga ataatattat caatgcagtc
    45721 atgattagct tgcatacatc ctagaaaacg actatttgca gcattaacaa tagcatcgat
    45781 ttttaacgtc gtgatatcac cttgccaaac aaagatattg tcacctttta tcggcttcaa
    45841 atccttcaaa tccgtaacat gttgacgatt caaatcactt aacatagcat cttgtacagc
    45901 taaatattca tcacttacag gtaatgccgg tctgacattt gctaagcctc gatataattc
    45961 ccacaaatct tcaaatgaag atggcattac caatacgtca ttgtcatttc tctctcgatg
    46021 catatcattg attaaatatt caagtctcgc tttatttgat tttaacgttt ccatttaagc
    46081 ctccggtaat gctaggaatg cttctttatc gacatcatcc aatttgaaca accaattttc
    46141 ttctggtttt tcagagttta aaattgtcgg ttcttcttcc gcttttgtat ttcgctcaat
    46201 aatcgttcct gacaatggcg tttgcacatc aatgaccgtt ttcgatgctt cgatactcac
    46261 aatttcatca tccactttaa cttcatctgg acttacgaat tcaacataac ctactgtccc
    46321 aatatcatct tgcaattcag gtgtcatact aaacacatac aaatctccta ctttttctac
    46381 ccataaataa ttggctaact ttttcatctt atcttcatcc tttctcttct atttaaaatg
    46441 ctacaaataa atttccgcga gtaattttaa tgttttacaa cgctgttcaa tacctggaat
    46501 aagcggtgct actaacacct catcaacttc aaacgtagca atgaaatcat ctaattgtgc
    46561 tttaaccttt tcttgtgtac ctgcaatgat gcgtgcttga tgtgcttgaa tcatctcttt
    46621 gtctcgatca ttaagcttat acttttgtgc tgtgtctact gaaggaaaat cttcaaattc
    46681 tgcaaattgt aatttaccta ataaccaaac atctaaggca tgttgtaatg ctgctacttc
    46741 cgcttcgtta tcagctacaa tgacaaatac agatgccatc acctttgcgt ccattttaat
    46801 cgttgatgct tggaaatgtt ttttgtaaat atcaatgtta tccttcgctg catgtatcgc
    46861 atttatatct ggtagcaaaa atgttccaac agaaagccct atacctagtt cggcagctat
    46921 tttggcagat gttgcgctac tacttaataa ccacatttct ggaaaatgat caatgtgtgg
    46981 ttggacacct aacgtatgcg cacttggttt atcctttatt gtaagataat cacgtaataa
    47041 cgaaatcgat tcatcgtaac tatcatatgt aggatttatt ccatctaaag cttgctttac
    47101 catagtagta cctggattat taccaatacc taaatcaata cgatttggat ataacgctgc
    47161 catcattcta aaatgctcag caattttata aggtcgatag tgcggcagca tcacaccacc
    47221 agagccaact cgtatgtgat ttgtctgcgc caatgtatgc atcatcaaaa gttctggact
    47281 actacacgca aacgctggta cattatgatg ttccgtaaac caaattcgct taaagccaag
    47341 tcgatctgct aattttgcaa gtgtcactga atcttgcaat gccttttgtg catccttacc
    47401 ttcatctatt aaggcatagt ctaatacgct taatttaacc aatccgtcat ctccaaactt
    47461 atcctgtcat gtcaaaccga cataacattt tagcgttctt aataccattc cctcttcata
    47521 tacccacgta tatgataacg tttttcaatt aacttttata tcttttcgcc tttatttttc
    47581 tttttcatat tcatattttc agacctacaa atgccattca ttagtttaat tttaaaaata
    47641 ctttccatgt accaattcta ttctatcaag actgttcatc attgattatt cattctttcc
    47701 atggtacttt gaaaacaata ttgaatagaa aaggaatcat agtatgtacc gatacaaacc
    47761 acttttacaa tcaatccact tacctaatgg catcaaaatt tcgaatcgct ttgtattatc
    47821 accaatgacc gtaaacacat caacaaaaga aggctatatt acaaaagaag acttggctta
    47881 tgctgcacgt cgctctaatt cagctggaat gcaagtcacc ggcgctgctt acattgaacc
    47941 ttatggtcaa ttgtttgaat atggctttaa tattgatcat gatgcttgta tccctggatt
    48001 aactaacatg gcatctacga tgaaacaaca tggtagcctc gctattatcc agctagcgca
    48061 tgctggtcgt ttttcaaatc aagccatttt aaattttgga aaggtgtatg gaccaagccc
    48121 tatgacattg cattcaccga ttgaacacga tgttatcgcc atgtctcatg agaaaatcaa
    48181 tagcatcatt cagcagtatc gcgatgctac attacgcgcg attaaagcag gttttgatgg
    48241 tgtggaaatt tcgattgcac aacgtttgct gattcaaaca tttttctcaa ctttttcgaa
    48301 taaacgtaca gaccattacg gtgccgactc attaaaaaat cgtgcaagat tatgtttaga
    48361 agttatgcgt gcggtacaag aagtgattga taaagaagca cctgacaatt ttattttagg
    48421 atttcgagca acgccagaag aaaccagagg cagtgattta ggatatacca ttgacgagtt
    48481 caaccaatta atcaattggg tcatggacgt ttcaaacatc caatacttag caatcgctag
    48541 ctggggtcga catatttatc aaaatacgtc tcgtacacca ggtgatcatt tcggtcgacc
    48601 agttaaccaa attgtctatg aacatttagc aggtcgtatc cctttaattg caagtggcgg
    48661 gattaattct ccagagtctg ctttagatgc tttacaacat gcagatatgg tcggcatgtc
    48721 ttcgcctttt gtcacagaac ctgactttgt gcataaactt gctgaacagc gaccacatga
    48781 tatcaatctt gaattttcta tggcagattt agaagattta gccattccac atgctgcttt
    48841 taaagatatt gttaaaatga tggattatgg ggaaggtcta aagaagcata cccgtgacgc
    48901 tttacgtcaa ttggaacaga attatcatga ctcatcgtct taagactatt tttcatggaa
    48961 atttaagaca gtgttataat gacatcaata tcaaaaataa ggagcctatt aaatcgtgac
    49021 tgaaattaaa gataaagtaa ttactaaaga tgcatttgca ttaccttaca caattattaa
    49081 agcaaaaaat caaccaacaa aaggcgctat tgtctacatc cacggtggtg gattaatgtt
    49141 cggtaaagct aacgatttat ccccacagta tatcgatatc cttacggaac attacgattt
    49201 aattcaacta agttatcgtt tattaccaga agtaagtctc gattgcatta ttgaggatgt
    49261 ctacgcatca ttcgatgcga ttcaatcaca atattctaat tgccctattt ttacctttgg
    49321 cagatcatca ggtgcatatt taagtctatt aattgcacga gacagagata ttgatggtgt
    49381 tattgatttt tatggttata gtcgcatcaa cactgaacct tttaaaacaa ctaatagtta
    49441 ttacgcaaaa atagctcaaa gcgtaaacga aacaatgatt gcacaactca cttcaccaac
    49501 accggttgtt caagatcaaa tcgcacaacg ttttttaatc tacgtttatg cacgtggcac
    49561 aggtaaatgg ataaatatga ttaacattgc tgattatact aattcaaaat ataatattgc
    49621 accagatgaa cttaaaacac ttccacctgt tttcattgcg cattgtaatg gcgattatga
    49681 tgttcccgtt gaggaaagcg aacatatcat gaatcatgtt ccacattcaa catttgaacg
    49741 cgtgaataaa aacgagcatg attttgatcg tagacctaat gacgaagcaa tcactattta
    49801 tcgtaaagtt gttgatttct taaacgctat aacaatgatg taatatatca ttattttaaa
    49861 aaactcaatt tatcatcatg ataaacagtt attcgatcaa ccatattatt catacaattt
    49921 attaagctca taaacagata cgcctgtccc tttagttttc gctaatgaga caggcgcttt
    49981 atttcacttt actattttta aattcaacac tgtgatgcta cttatatctg atgtgccgtg
    50041 ataacagcac gatttacata gcatattaaa ttcagaagat gaatttaact tgctttcaat
    50101 tgtgttcctt tactttcagt cgcttcaaca cgcaacaagt catttataat acctgtatag
    50161 aagttggcaa gttctgcacc tttacgtttg aaatccagga agtccacacc gataaaatcg
    50221 acatgatccc atccttgtat gattggttta acttgccaga tacctctgcg tgtggcaggt
    50281 tcatcattcg taacattaac aaatggttga ttggacggat gtaatgacga aatcactggt
    50341 acgacaccat catttttacg ccattcttct cttgcatcat gaccaataat tctactcgtt
    50401 gtagccatta agaaaaatgt acctaaatca ggattttcat aacctaatgg accagtatga
    50461 gatgatacac ctgtataagt cgtatacgta atattaggat tcatacttgt catgttgttc
    50521 aattttgcag agccatctaa cgttaaatca taggcagcat tgtcgtctga tgtccaaatt
    50581 ttgcttttac taacgcgttt tatatagtca atgtaactct catttggtaa ttgtttaaag
    50641 ccccattgcg ttaatcctaa atcgatattc gaatacttgt tacccataaa tcgatttaaa
    50701 gcgaacatga tttttctaac agcttctgta tttccaaact tatcagctgc ttgtgaacca
    50761 ttatgtggtg ttgctaatgt tgtgattgat gcaaccatat tgttatgacc accagtgaat
    50821 aatggtgata tttctccacc atgcgcttta tgataggcaa tttcttcttt gttaccattt
    50881 cttaaaaact cttccattaa acgaattgtt tgaccaccca tactatgccc tacaagatgt
    50941 acctttttac caggttccca attaggcatg attcctttat aagtcttacc atagcgctca
    51001 tgtccgtatt tagctgcatg tgctgcgcca taatctacgc gaccaccttt aatgtaataa
    51061 taaagttcta cagcgcgatc atagttacta ccaaatgcac ttacacttgc ttgatgtaca
    51121 ttatagcctt gctttctcaa ttcttcgata actttaaatt tatttccacc ccaataattt
    51181 ggatataaag caggtgcatt atcgcctact aatcctaaaa atccatgtac aaaaacaact
    51241 ggatatttgt taagtggttg tacttgattc gctttaagtg gtcgaacctt tttagcatct
    51301 gctgctgcat ttgtagtcga acgtgtttta ggcgcttgaa gcgcatcttt gattagttgt
    51361 ttattatcag tatttttatc tgtcggtttc tctggggaat tttgaccatc gatatgttta
    51421 cttaaatcgc caacttgtgg tttctgttca ctttggcgaa cagtatcatc ttgctttgca
    51481 tctatgattt gatgcgcagg ttgatgtgta tcttgttgat ttggatgttt gtccgtggtt
    51541 gcatcttgtg cttttgttga tttaggtgca gttttatcat tagacggggg tgtagttgaa
    51601 gttggtgctg ctttatcatg attgggtatt accttttgtg ccggaatgga tggttgtgat
    51661 ttatcaacat cattcgattg tgacgttgtc gattgtgatc cattacgttc agcatgatgg
    51721 gtattttgaa cctgatcatt ttgacgttca gtattattag tcgcattatt ttggtgtgac
    51781 tgtttagaac cttgtcgttc agaatcttgc tttacttgat tatcattttg cattctgatt
    51841 gttttactat tttgtgttga ttgatgcttc gatgattgaa gcgtatcttt accttctgtc
    51901 actgttccac ttttaccatt ctctttatgc atatcgtcta attgctttcc tgactgcatt
    51961 tgatgtgacg ttatcgcatt cccttgttct cccggttgat ttagtgtttc tttttgtgcc
    52021 gctgcattag ttgatgtttt ttccgaggct tgtgcttcat gtgatgacac aacaaacatt
    52081 gtagccgcta acactgacac cacgcctatt gaatactttc taatactata ctttctttct
    52141 tcttgtcctc ttaacatata atcacctcaa aagtattttt ttgaccccct cttgagaatg
    52201 aaaaacaact gcaccttcaa tataacatga catatattat tttttgatta agactacaaa
    52261 attaataaaa atttaataat acgatattaa atcgatacta atcatttaca aatatgtgta
    52321 tttccaaata at
//
LOCUS       DAKTLH010000004        51561 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.004, whole genome shotgun sequence.
ACCESSION   DAKTLH010000004 DAKTLH010000000
VERSION     DAKTLH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 51561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51561
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.004"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            28..936
                     /locus_tag="O0394_000141"
     CDS             28..936
                     /locus_tag="O0394_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0498257.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            1335..2276
                     /locus_tag="O0394_000142"
     CDS             1335..2276
                     /locus_tag="O0394_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCY0498258.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            2397..2951
                     /locus_tag="O0394_000143"
     CDS             2397..2951
                     /locus_tag="O0394_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCY0498259.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGIK"
     gene            3023..3793
                     /locus_tag="O0394_000144"
     CDS             3023..3793
                     /locus_tag="O0394_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0498260.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIGEYAISKNH
                     KPIILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(3965..4762)
                     /gene="fdhD"
                     /locus_tag="O0394_000145"
     CDS             complement(3965..4762)
                     /gene="fdhD"
                     /locus_tag="O0394_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCY0498261.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPMQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            5052..5834
                     /gene="modA"
                     /locus_tag="O0394_000146"
     CDS             5052..5834
                     /gene="modA"
                     /locus_tag="O0394_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0498262.1"
                     /translation="MKLKRLFAVVIAMLLVLAGCSNSNDNNESKKDDADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA"
     gene            5848..6519
                     /gene="modB"
                     /locus_tag="O0394_000147"
     CDS             5848..6519
                     /gene="modB"
                     /locus_tag="O0394_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCY0498263.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYRRKGSWVKLLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            6520..7125
                     /locus_tag="O0394_000148"
     CDS             6520..7125
                     /locus_tag="O0394_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0498264.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            7295..8299
                     /locus_tag="O0394_000149"
     CDS             7295..8299
                     /locus_tag="O0394_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HCY0498265.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKYRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            8330..8836
                     /locus_tag="O0394_000150"
     CDS             8330..8836
                     /locus_tag="O0394_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HCY0498266.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFDTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     DGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(8838..9332)
                     /gene="moaC"
                     /locus_tag="O0394_000151"
     CDS             complement(8838..9332)
                     /gene="moaC"
                     /locus_tag="O0394_000151"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCY0498267.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTLIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSNQ"
     gene            9395..10654
                     /locus_tag="O0394_000152"
     CDS             9395..10654
                     /locus_tag="O0394_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCY0498268.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETKTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQKDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFVDGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            10651..11136
                     /gene="mobB"
                     /locus_tag="O0394_000153"
     CDS             10651..11136
                     /gene="mobB"
                     /locus_tag="O0394_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCY0498269.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            11150..11596
                     /locus_tag="O0394_000154"
     CDS             11150..11596
                     /locus_tag="O0394_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HCY0498270.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            11602..11835
                     /gene="moaD"
                     /locus_tag="O0394_000155"
     CDS             11602..11835
                     /gene="moaD"
                     /locus_tag="O0394_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HCY0498271.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            11842..12441
                     /gene="mobA"
                     /locus_tag="O0394_000156"
     CDS             11842..12441
                     /gene="mobA"
                     /locus_tag="O0394_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCY0498272.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            12461..13483
                     /gene="moaA"
                     /locus_tag="O0394_000157"
     CDS             12461..13483
                     /gene="moaA"
                     /locus_tag="O0394_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCY0498273.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(13878..14048)
                     /locus_tag="O0394_000158"
     CDS             complement(13878..14048)
                     /locus_tag="O0394_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498274.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNGMECDINVKEPLKIYYV
                     VILKSGIEMIFS"
     gene            14174..14524
                     /gene="sarV"
                     /locus_tag="O0394_000159"
     CDS             14174..14524
                     /gene="sarV"
                     /locus_tag="O0394_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HCY0498275.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(14626..15837)
                     /locus_tag="O0394_000160"
     CDS             complement(14626..15837)
                     /locus_tag="O0394_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0498276.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFLVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(15830..16270)
                     /locus_tag="O0394_000161"
     CDS             complement(15830..16270)
                     /locus_tag="O0394_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HCY0498277.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLREAHND"
     gene            16432..17196
                     /locus_tag="O0394_000162"
     CDS             16432..17196
                     /locus_tag="O0394_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0498278.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(17188..17439)
                     /locus_tag="O0394_000163"
     CDS             complement(17188..17439)
                     /locus_tag="O0394_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498279.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLVIIHMGILAHSTYVYLY"
     gene            17828..19093
                     /gene="femX"
                     /locus_tag="O0394_000164"
                     /gene_synonym="fmhB"
     CDS             17828..19093
                     /gene="femX"
                     /locus_tag="O0394_000164"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HCY0498280.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMKTSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            19210..22377
                     /locus_tag="O0394_000165"
     CDS             19210..22377
                     /locus_tag="O0394_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCY0498281.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVATIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(22564..22881)
                     /gene="mspA"
                     /locus_tag="O0394_000166"
     CDS             complement(22564..22881)
                     /gene="mspA"
                     /locus_tag="O0394_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HCY0498282.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(23126..23290)
                     /locus_tag="O0394_000167"
     CDS             complement(23126..23290)
                     /locus_tag="O0394_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498283.1"
                     /translation="MSLENQLAELKYDYVRLQGDIEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            23423..24331
                     /locus_tag="O0394_000168"
     CDS             23423..24331
                     /locus_tag="O0394_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCY0498284.1"
                     /translation="MTEQFTMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFVMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVYVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     TFIGMTTNLTIIISIIILYVYQAVFV"
     gene            24502..24633
                     /locus_tag="O0394_000169"
     CDS             24502..24633
                     /locus_tag="O0394_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498285.1"
                     /translation="MYGAYLIYEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(24750..25613)
                     /locus_tag="O0394_000170"
     CDS             complement(24750..25613)
                     /locus_tag="O0394_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HCY0498286.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(25794..26681)
                     /locus_tag="O0394_000171"
     CDS             complement(25794..26681)
                     /locus_tag="O0394_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0498287.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            26747..26842
                     /locus_tag="O0394_000172"
     CDS             26747..26842
                     /locus_tag="O0394_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498288.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            26868..29003
                     /locus_tag="O0394_000173"
     CDS             26868..29003
                     /locus_tag="O0394_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCY0498289.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSL
                     TVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            29116..30450
                     /locus_tag="O0394_000174"
     CDS             29116..30450
                     /locus_tag="O0394_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCY0498290.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(30642..31031)
                     /locus_tag="O0394_000175"
     CDS             complement(30642..31031)
                     /locus_tag="O0394_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498291.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            31388..31696
                     /gene="rpsJ"
                     /locus_tag="O0394_000176"
     CDS             31388..31696
                     /gene="rpsJ"
                     /locus_tag="O0394_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCY0498292.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            31724..32386
                     /gene="rplC"
                     /locus_tag="O0394_000177"
     CDS             31724..32386
                     /gene="rplC"
                     /locus_tag="O0394_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCY0498293.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            32413..33036
                     /gene="rplD"
                     /locus_tag="O0394_000178"
     CDS             32413..33036
                     /gene="rplD"
                     /locus_tag="O0394_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCY0498294.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            33036..33311
                     /gene="rplW"
                     /locus_tag="O0394_000179"
     CDS             33036..33311
                     /gene="rplW"
                     /locus_tag="O0394_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCY0498295.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            33344..34177
                     /gene="rplB"
                     /locus_tag="O0394_000180"
     CDS             33344..34177
                     /gene="rplB"
                     /locus_tag="O0394_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCY0498296.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            34244..34522
                     /gene="rpsS"
                     /locus_tag="O0394_000181"
     CDS             34244..34522
                     /gene="rpsS"
                     /locus_tag="O0394_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCY0498297.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            34551..34904
                     /gene="rplV"
                     /locus_tag="O0394_000182"
     CDS             34551..34904
                     /gene="rplV"
                     /locus_tag="O0394_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCY0498298.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            34928..35581
                     /gene="rpsC"
                     /locus_tag="O0394_000183"
     CDS             34928..35581
                     /gene="rpsC"
                     /locus_tag="O0394_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCY0498299.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            35584..36018
                     /gene="rplP"
                     /locus_tag="O0394_000184"
     CDS             35584..36018
                     /gene="rplP"
                     /locus_tag="O0394_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCY0498300.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            36008..36217
                     /gene="rpmC"
                     /locus_tag="O0394_000185"
     CDS             36008..36217
                     /gene="rpmC"
                     /locus_tag="O0394_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCY0498301.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            36241..36504
                     /gene="rpsQ"
                     /locus_tag="O0394_000186"
     CDS             36241..36504
                     /gene="rpsQ"
                     /locus_tag="O0394_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCY0498302.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            36536..36904
                     /gene="rplN"
                     /locus_tag="O0394_000187"
     CDS             36536..36904
                     /gene="rplN"
                     /locus_tag="O0394_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCY0498303.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            36940..37257
                     /gene="rplX"
                     /locus_tag="O0394_000188"
     CDS             36940..37257
                     /gene="rplX"
                     /locus_tag="O0394_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCY0498304.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            37284..37823
                     /gene="rplE"
                     /locus_tag="O0394_000189"
     CDS             37284..37823
                     /gene="rplE"
                     /locus_tag="O0394_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCY0498305.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            37846..38031
                     /locus_tag="O0394_000190"
     CDS             37846..38031
                     /locus_tag="O0394_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCY0498306.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            38063..38461
                     /gene="rpsH"
                     /locus_tag="O0394_000191"
     CDS             38063..38461
                     /gene="rpsH"
                     /locus_tag="O0394_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCY0498307.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            38486..39022
                     /gene="rplF"
                     /locus_tag="O0394_000192"
     CDS             38486..39022
                     /gene="rplF"
                     /locus_tag="O0394_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCY0498308.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            39053..39412
                     /gene="rplR"
                     /locus_tag="O0394_000193"
     CDS             39053..39412
                     /gene="rplR"
                     /locus_tag="O0394_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCY0498309.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            39433..39933
                     /gene="rpsE"
                     /locus_tag="O0394_000194"
     CDS             39433..39933
                     /gene="rpsE"
                     /locus_tag="O0394_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCY0498310.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            39950..40129
                     /gene="rpmD"
                     /locus_tag="O0394_000195"
     CDS             39950..40129
                     /gene="rpmD"
                     /locus_tag="O0394_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCY0498311.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            40146..40586
                     /gene="rplO"
                     /locus_tag="O0394_000196"
     CDS             40146..40586
                     /gene="rplO"
                     /locus_tag="O0394_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCY0498312.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            40586..41878
                     /gene="secY"
                     /locus_tag="O0394_000197"
     CDS             40586..41878
                     /gene="secY"
                     /locus_tag="O0394_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCY0498313.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            41895..42542
                     /locus_tag="O0394_000198"
     CDS             41895..42542
                     /locus_tag="O0394_000198"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCY0498314.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            42735..42953
                     /gene="infA"
                     /locus_tag="O0394_000199"
     CDS             42735..42953
                     /gene="infA"
                     /locus_tag="O0394_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCY0498315.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            42985..43098
                     /gene="rpmJ"
                     /locus_tag="O0394_000200"
     CDS             42985..43098
                     /gene="rpmJ"
                     /locus_tag="O0394_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCY0498316.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            43121..43486
                     /gene="rpsM"
                     /locus_tag="O0394_000201"
     CDS             43121..43486
                     /gene="rpsM"
                     /locus_tag="O0394_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCY0498317.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            43510..43899
                     /gene="rpsK"
                     /locus_tag="O0394_000202"
     CDS             43510..43899
                     /gene="rpsK"
                     /locus_tag="O0394_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCY0498318.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            43974..44918
                     /locus_tag="O0394_000203"
     CDS             43974..44918
                     /locus_tag="O0394_000203"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCY0498319.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            44935..45303
                     /gene="rplQ"
                     /locus_tag="O0394_000204"
     CDS             44935..45303
                     /gene="rplQ"
                     /locus_tag="O0394_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCY0498320.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            45841..46650
                     /locus_tag="O0394_000205"
     CDS             45841..46650
                     /locus_tag="O0394_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCY0498321.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPYDEMHRRVSEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDNAEELTRIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            46647..47507
                     /locus_tag="O0394_000206"
     CDS             46647..47507
                     /locus_tag="O0394_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCY0498322.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKKKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            47497..48303
                     /locus_tag="O0394_000207"
     CDS             47497..48303
                     /locus_tag="O0394_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCY0498323.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWVFALILFIMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTISLTMIIPIAIILFVLKYSGV"
     gene            48308..49111
                     /gene="truA"
                     /locus_tag="O0394_000208"
     CDS             48308..49111
                     /gene="truA"
                     /locus_tag="O0394_000208"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCY0498324.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLGKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            49351..49788
                     /gene="rplM"
                     /locus_tag="O0394_000209"
     CDS             49351..49788
                     /gene="rplM"
                     /locus_tag="O0394_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCY0498325.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            49802..50200
                     /gene="rpsI"
                     /locus_tag="O0394_000210"
     CDS             49802..50200
                     /gene="rpsI"
                     /locus_tag="O0394_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCY0498326.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            50745..>51561
                     /locus_tag="O0394_000211"
     CDS             50745..>51561
                     /locus_tag="O0394_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCY0498327.1"
                     /translation="MRGDQRTQGEKSRKEGGKIFGSGSRAPIAISILVKDGSYNHDIY
                     YNDIGEYLTREQKLDTLMKHQSIVNLKSLNVLPDKNNDWINQRDINYENYLPMYDSKD
                     IENSIYLDQFNGVNSARDNWVTNFSNEKALVNAKLLVDNYNSEIDRLIDILDSRERIN
                     LVNKDETFISWTRGLTQKFSKGKNISINPERIVKFMHRPFTKKWIVYDKNIMEMPSRY
                     YNIMENTGQVIYIQGQGMNKEFSAMITDILPNFQFIGNGKGFATYKGKDSLRLV"
BASE COUNT        18643 a         7623 c         9831 g        15464 t
ORIGIN      
        1 gataatcatt ttcaatagga ggaaattatg aaaaaactat tattaccatt aataattatg
       61 ttattagtgt tagctgcgtg tgggaaccaa ggtgaaaaaa ataacaaagc tgaaactaaa
      121 tcttataaaa tggacgatgg caaaacggta gatattccga aagaccctaa acgcattgca
      181 gtagttgcgc caacatatgc tggtggactt aaaaaattag gtgcaaacat tgtagctgta
      241 aatcaacaag tcgatcaaag caaagtatta aaagataaat ttaaaggtgt tacaaaaatt
      301 ggtgatggcg atgtagaaaa agttgctaaa gaaaagccag atttaattat tgtatactct
      361 actgacaaag atattaaaaa atatcaaaaa gtagcaccaa cagtagttgt tgactataat
      421 aagcataaat atttagaaca acaagaaatg ttagggaaaa ttgttggtaa agaagataaa
      481 gtaaaagctt ggaagaaaga ttgggaagaa acaactgcta aagacggtaa agaaattaaa
      541 aaagcaattg gacaagatgc aacagtgtca ttgtttgatg aatttgataa aaaattatac
      601 acttacggcg ataactgggg tcgtggtgga gaagtattat atcaagcatt tggtttgaaa
      661 atgcaaccag aacaacaaaa gttaactgca aaagcaggtt gggctgaagt gaaacaagaa
      721 gaaattgaaa aatatgctgg tgattacatt gtgagtacaa gtgaaggtaa acctacacca
      781 ggatacgaat caacaaacat gtggaagaat ttgaaagcta ctaaagaagg acatattgtt
      841 aaagttgatg ctggtacata ctggtacaac gatccttata cattagattt catgcgtaaa
      901 gatttaaaag aaaaattaat taaagctgca aaataattca gctatataag ttagtgaaat
      961 gagagtctga aacatatcaa tcttttgata ttgtattagg ctcttatttt tatagctaga
     1021 aagttagata tttgtatttt tttaaataat aagtgccgtt gttatcgttc aatttaatta
     1081 atgatagatt agtattatta tagctaaagt agtatacctg agaaaatagc tcaatgtatc
     1141 tctttattaa taagttatat cataattatt ttagtgcata ctttatggaa gggatatcag
     1201 ggaatggctt tcaattaaag aagaggttta aaaggattac aacagaatgt tatgattttg
     1261 tagaaagata tataacaacg ttttataaaa acataatatt gttaatggaa aatgaaatgt
     1321 aagggggatt tcgagtgact aagaaagttt attttaacca cgatggtggt gtagatgatt
     1381 tagtatctct atttttatta ttacaaatgg aaaacgttca attgataggg gtcagtacaa
     1441 ttggtgctga ttgttattta gagccatctt tgagcgcatc agtaaaaatt attaatcgtt
     1501 tttcaaatga agatattcaa gttgcgccat catatgaacg aggaaaaaat ccatttccta
     1561 aagaatggcg tatgcatgcc ttttttatgg acgcattgcc aattttaaat gagccagtca
     1621 aacatgttgc ttcaaatgtg agcgacaaag aagcctttga agacattatt caaactttaa
     1681 agagacaatc agaaaaagta acattattat ttacaggccc gcttacagat ttagcaaaag
     1741 cactacaaaa agattcatct atcgttcagt atatagaaaa attagtttgg atgggtggca
     1801 cctttttacc aaaaggaaat gttgaagaac ctgagcatga tggttctgca gaatggaatg
     1861 catattggga tccagaagcg gttaaaattg tttttgatag cgatatagag attgatatgg
     1921 ttgctttaga aagtacgaat caagtaccgc taacgttaga tgttagacaa agatgggcga
     1981 atgaacgcca atatacgggc attgattttt taggagtaag ttatgcagcg gtaccaccat
     2041 taacacactt tataacaaat tctacttact ttttatggga tgttttaacg actgcttata
     2101 ttggtaacaa ggacttggtt cattcaattg agaaaaaagt cgatgtaata agttatggac
     2161 caagtcaagg taagacattt gagtgtaaag atgggcgcaa aattaatgtc ataaatcatg
     2221 tagataacaa cgcatttttt gattatataa ctgcacttgc taaaaaagta aattaacagc
     2281 tgtgtagaat aattaaggtt ttaatttata tagaacaact tattgtaaac ttttcatttc
     2341 ttaaagttta caatggtgct ataataatgg tcatgaaata cgaaaggaag taaaaaatga
     2401 caacaaaaca gttagtatat acagctttaa tgacagcgat tatcgctatt ttaggattgg
     2461 taccggtaat tccactacca ttttcttcag taccaattgt acttcaaaac attggtattt
     2521 tcttagcagg tgtgatttta ggacgtaaat atggcacatt aagtgttatc gtctttttat
     2581 tattagtagt tgctggcttg ccattgttat caggtggtcg cggtggcatc ggtgtattcg
     2641 caggtccttc agcagggttt ttactattat atccagttgt agcattcatg attggggcga
     2701 ttcgagatag attcatcaat gaaattaatt tctggatttt attcgttggt attttagttt
     2761 ttggtgttat agcattagat gttattggta cattgattat gggcatgatt attaacatac
     2821 catttacgaa agctatttca atttcattag cttatttgcc tggtgatata ttaaaagcaa
     2881 ttgtagcaag tttgattggt acagctttac ttaatcactc gcagtttcgt caaattatgg
     2941 gaataaaata atcatattta agatagtaaa gtaattgaat aagttgcttt gaaatttata
     3001 aaagtgaaag gagtaggtgt caatggctag tataagtatg tcagatatat attgtaacgg
     3061 cactatattt gaaaatgacg acgagcagtt gatttattta acgccttctt ttccacaacg
     3121 atacacaagt aacacatgga tatataaaaa gacgcctacc caagagcgat ggctgaaaga
     3181 cttagaacgt caacatcaat tacatacaaa tcaaggttca aatcattatg cgtttagttt
     3241 cccggaaaat gaacaacttg ataatcattg gatggctatg tttaaagata tgaattttga
     3301 actaggtatt atggaattgt atgccataga aagtgatgcg cttgccaatt tgccgcgtaa
     3361 ctctgacgtt gaaattgcca tcgttgacga gtcgcatata gatgcctatt taaaagttgc
     3421 atatcagttt agtttgccat ttggaaaaga ctatgcagat gcacatgaag aaatggtaag
     3481 ggaacattat caaaaagatg tgattaaacg cttagtagct tatttaaata atgaacctat
     3541 tggcgttgta gatgtcattg aaagtgaaaa ttacattgaa ttagatggat ttggtgtatt
     3601 agaacaattt cggcaccaag gaattggatc tacaattcaa tcgttgatag gtgaatacgc
     3661 catatcaaaa aatcacaaac caatcatatt agttgcagat ggtgaagata cagcaaaaga
     3721 tatgtatgca aagcaaggtt atgtctatca atcgttttgt tatcaaatat taaaagaaga
     3781 tattggaaat taacgattga tgataggttt gtattagaat aaaaaagcag taagctggtt
     3841 tcgagtagaa atcaacttac tgctttttaa attgttttga gctacttata cttataaaaa
     3901 tagtgcgttt aaattgttga ttcatgtaga atatcgttca ttatgacaca ctataatgaa
     3961 tatgttattg ttcagaatca atgatacgtt ctggatgact gtatatatta aagccaccat
     4021 ttcgaataaa tccaactgcc gtaatattta ggtcattagc taaggttaca gcaagcgttg
     4081 tcggagctga tttagataaa atgacgccaa caccaatttt tgcggcttta attaaaattt
     4141 ctgatgaaat acgtccacta aaaattaata ctttatctcg gacagtaata tgtcgctgaa
     4201 tacaaaatcc atataattta tctagagcgt tatgtctacc aatgtcttgt cgatgtacaa
     4261 aaaatgtcaa accatcgctt atagcagcat tatgtaagcc acctgtttct tggtaaatat
     4321 gacttgcact ttgtaatcga gtcatcatgt taataatttg cattggagtt aaagtgattt
     4381 tagacataga tgttttagcg atagcagcat cattttgaaa ataaaactca cgactctttc
     4441 cgcaacaaga tgcaatcatt cgttttgtgg aatattgaaa gcgatcgcct aaatctttat
     4501 taagttcaac atgggcaaaa cctttactat catcaatcag tacagatttt aattcatctc
     4561 gctttaaaat ggcaccttcc gaagccagaa atccaatgac taactcctca aggtttgttg
     4621 gactgcatat aacagtcgca aattcttcac cattcaccat aattgtaagt ggaaattctg
     4681 tcacatattg atctgttgta ttgaataatt ttccatcttc atatctaaca attggttgac
     4741 ctaaagatac atctttgttc attatctaac ccctttaatt agcttaaact ttattttaaa
     4801 gcaatttgct taaaatttta acatatttgc ttaagtttga aatttgattg ataaaaatta
     4861 atagcgagca atctgtttga tttaaattga attcgagaat atacatacta gggcatcaat
     4921 taataaatat caatcttatg caaatttgac aattgtttga atcaatatat aaacaggcaa
     4981 cggttctttt caaatataat agtaagtgta taatgaaaat gtaaatatta ttaaaaatgg
     5041 gggttcactc aatgaaattg aaacgtttat ttgctgttgt gattgcaatg cttttagtat
     5101 tagctggttg ctctaattct aacgataata atgaaagtaa aaaagatgac gcagacaatg
     5161 gtaagaaaca agagattcaa gttgcagcgg cagcaagttt aacagatgta accaagaaat
     5221 tagcttcaga atttaaaaaa gagcataaaa atgctgatat taaatttaac tatggtggat
     5281 caggggcatt aagaaaacaa attgaatcag gcgcacctgt tgacgtattt atgtctgcaa
     5341 atactaaaga tgtagatgca ttaaaagaca agaataaagc gcatgataca tataaatatg
     5401 cgaaaaatag tctagtatta attggtgata aagattcaaa ttacacttca gtaaaagact
     5461 taaaagacaa tgataaatta gcattaggtg aagtgaaaac tgtaccagca ggaaaatatg
     5521 cgaaacagta tttagataac aataacttat ttaaagaagt cgaaagtaaa atcgtttatg
     5581 ctaaagatgt aaaacaagta ttaaattatg ttgaaaaggg taatgcgaaa caaggttttg
     5641 tgtataaaac tgacttatat aaacaaaata aaaaaattga tactgtaaaa gtaattaaag
     5701 aagtagaact taagaagcca atcacatacg aagctggtgc tacatcagat agtaaattag
     5761 caaaagagtg gatggaattc ttaaaatcag ataaagctaa agaaatacta aaagaatacc
     5821 actttgcagc ataaggagtt gtaatccatg cctgacttaa cacctttttg gatatcaata
     5881 cgagttgctg taatcagtac gattattgta acggttttag gtatttttat atctaaatgg
     5941 ttgtatcgtc gtaagggttc gtgggttaaa ttattggaaa gtttattgat attacctatt
     6001 gttttgccgc caacggtatt aggttttatt ctattaatca tcttctcgcc aagaggacca
     6061 atcggtcaat tctttgcgaa tgtactacat ttacctgtag tgttcacttt gacaggtgct
     6121 gtgatagcat ctgtcattgt tagttttcca ctaatgtatc aacatactgt gcaaggcttc
     6181 agaggtatag acacgaaaat gattaataca gctagaacga tgggagcaag tgaaacgaaa
     6241 attttcctca aattaatttt accattagct aaacgctcta ttttagcagg tataatgatg
     6301 agttttgctc gtgcattagg tgagtttggt gctacattaa tggttgcagg atatattcca
     6361 aataaaacga atacactacc tttagaaata tacttcttag tggaacaagg tagagaaaat
     6421 gaagcgtggt tatgggtatt agtgctagtc gcattctcta ttgtggttat atctacaatt
     6481 aatttattga ataaagataa atataaggag gtcgactaga tgcttaaaat caatgtgaaa
     6541 tatcaattaa agaacacttt aattcgcatc aatatagatg atactgaacc aaaaatttat
     6601 gcagttcgtg gtccatctgg cattggtaaa actactgttt taaatatgat tgccggatta
     6661 cgtaaagcag atgaagctat tatcgaagtg aatgggcaat tacttactga tacggcaaaa
     6721 aacgtgaatg ttaaaattca acaacgacgt attggatatc tgtttcaaga ctaccaattg
     6781 tttcctaata tgacggtcta taaaaatatt acttttatgg ctgaaccatc tgaacacatc
     6841 gatcaattaa ttcaaacttt aaacattgat catttgatga aacaatatcc tatgacattg
     6901 tcaggtggag aggcacaacg tgtagcactt gcacgtgcac ttagcacgaa accagattta
     6961 attttattag atgaaccttt ttctagtttg gatgatacta caaaagatga gagtattaca
     7021 ttagttaaac gtattttcaa cgaatggcaa ataccaatca tatttgtgac acattcaaac
     7081 tatgaagcag aacaaatggc tcatgaaatt attacaattg ggtaatcatt tatttgccat
     7141 taaagagttt agaacgtatt taaaattgta gaagtgaatg cttctatcag cattttaatg
     7201 atgttttaaa ctctttttta ggggcagttt ttttgagaga cattgacgcg cgtcatataa
     7261 tgaaagtaat gataaaaaga aaggataact taatgtgagt caagaacgtt attcaaggca
     7321 aattttattt aaacaaatag gtgaaatagg tcaaagcaaa ataaatcaaa aatgtgcgtt
     7381 gattattggt atgggcgcat taggtacaca tgtggccgaa ggacttgtta gagcaggcat
     7441 tgccaaacta atcattgttg atagagatta tattgaattt agtaatttac aaagacaaac
     7501 attgtttact gaagaagatg ctttgaaaat gatgcctaag gtggttgcag ctaaaaagca
     7561 tttgctagcg ttacgtagtg atgttgatat tgatgattat attgcccatg tggattatta
     7621 ttttttggaa acacatggac aggacgttga cgttattatt gatgcaaccg ataactttga
     7681 aacacgacaa ctgattaatg attttgcata taaatatcgt ataccttgga tttatggtgg
     7741 tgttgtacag agtacatata cagaagctgc atttatacct ggtaaaacac cttgctttaa
     7801 ctgtttggta ccacaattgc cagcattaaa tttaacatgt gatacagtag gggtcattca
     7861 acctgccgtg acgatggcaa caagtttaca attaagagat gcgatgaaag tattaacgga
     7921 acaaccaatt gacacaaaaa taacttatgg cgatatttgg gaaggtagtc attattcatt
     7981 tggtttcagt aaaatgcaac gttcagactg tacaacttgt ggagatgtac caagttatcc
     8041 gtatttaaac aagaatgaac aacgttatgc aacattgtgt ggtagagaca ctgtacagta
     8101 tgaaaatgca tcaattacac acgacattct tgttcaattt ttaaaacaac atcagttaaa
     8161 ttatcgcagt aattcgtata tggttatgtt tgaatttaaa ggacaccgca ttgttgcttt
     8221 taaaggtgga aggtttttaa tacatggcat gacacgcaca tcagatgcca cacatctaat
     8281 gaatttattg tttggataaa aaaagataag acaaaaggag tgtaatatta tgggcgaaca
     8341 tcaaaacgtt aaattgaatc gtacagttaa agcagccgta ctaacggtat cagatactag
     8401 agactttgat acagataaag gtggtcaatg cgtgcgccaa ctattacaag cagatgacgt
     8461 tgaagtgagt gacgcacatt atacaattgt gaaagatgaa aaagtagcca tcacgacgca
     8521 ggtgaagaag tggttagaag aagatattga tgtcatcatt acgactggtg gaacaggtat
     8581 tgcacaacgt gatgtgacga ttgaagcagt aaaaccactt ttaactaaag agatagatgg
     8641 ctttggggaa ttgtttagat atttgagtta tgttgaagat gttggcacgc gtgcattatt
     8701 gtctcgtgct gtagcaggta cagttaataa taaattgata ttttcgattc caggatcaac
     8761 aggcgcagtt aaattagcat tagaaaagct cattaaacca gaattaaatc atctgattca
     8821 tgagcttaca aaataattta ttgatttgat tggcgttgaa aatctccaga tttaccgcca
     8881 gacttgcttt caaggtaggt ttcgccaata atcatacctt tatcaactgc tttcgtcatg
     8941 tcgtaaatgg ttaaagccgt tgctgatgca gcggttaaag cttccatttc aacaccggtt
     9001 ttgccagttg tagagacagt tgtttgaatg tttaaagtat aaaggggtgc atttgtttca
     9061 tcccagctga agtgaacatc tatgccagtc aatggtaatg gatggcacat cggaataagt
     9121 gttgatgtat ttttggcagc cataatacca gcgatttgag cagtgttcaa tacattgcct
     9181 ttggtattgg tgttattaga aatctgctta aaaatagttt catttacagt aatgcttgaa
     9241 tgagcgacag cagttctttt tgtaatttgt ttgtctgata catcgaccat tttggcgtgg
     9301 ccttgttgat taatatgagt aaactcagtc attttacccc tcctagtgca tctagtatat
     9361 catgaaaaaa taaaagtttt ggagatgatt tttaatggta gtagaaaaaa gaaacccaat
     9421 cccagttaaa gaagcaattc aacgtatcgt taatcagcag agttcaatgc cggcaattac
     9481 ggtagcactt gaaaaaagtc taaatcatat cttagcagaa gatattgtag ctacttatga
     9541 tataccaagg tttgataaat caccttatga tggttttgca attcgcagtg ttgattcaca
     9601 aggggcaagt ggtcagaatc gcattgagtt taaagtgatt gatcatattg gtgcaggttc
     9661 agtttctgat aaattagttg gggatcacga agcggtgcgt attatgactg gagcacaaat
     9721 acctaatggc gcagatgctg ttgttatgtt tgaacaaacg attgaactag aagatacatt
     9781 tacaattcgt aaaccatttt caaaaaatga aaatatatct ttaaaaggtg aagaaacaaa
     9841 gacaggcgat gttgttctaa aaaaaggaca agtaattaat ccaggggcta tcgcggtcct
     9901 tgcaacatat ggctatgcag aggttaaagt tattaagcaa ccgagtgtcg ctgttattgc
     9961 aacaggaagc gaattattag atgttaatga tgtattagaa gatggaaaaa ttcgtaactc
    10021 taatggccca atgattcgtg ccttagcaga aaaattaggt cttgaagttg gtatttacaa
    10081 aacacaaaaa gatgatttag atagtggcat ccaagtcgtt aaagaagcta tggaaaaaca
    10141 tgatatcgtt attacaacgg gcggagtttc tgttggagat tttgactatt tacctgagat
    10201 ttataaggct gtaaaggcgg aagtgttatt taataaagta gcaatgcgtc ctggtagcgt
    10261 aacaacggtt gcatttgtag atggaaagta tttgtttgga ttatctggaa atccatcagc
    10321 ttgttttaca ggatttgaac tatttgtgaa accagctgtt aaacatatgt gtggcgcact
    10381 agaagtcttc ccgcaaataa ttaaagcaac attaatggaa gattttacca aggcaaaccc
    10441 attcacacga tttatacgtg ctaaagcaac gttaacaagt gctggagcta ctgtagtacc
    10501 ttcaggattc aataaatcag gtgcggttgt agcgattgca catgctaact gtatggtcat
    10561 gttaccagga gggtcacgtg gttttaaagc ggggcataca gtagatatta tattgactga
    10621 atctgacgct gctgaagagg aacttctttt atgattttac aaattgtagg ttacaaaaag
    10681 tctggtaaga caacattgat gaggcatatt gtctctttct taaagtcaca tggttataca
    10741 gttgctacta ttaaacatca tgggcatggt aaggaagata ttcaattaca ggattcagac
    10801 gtcgatcaca tgaagcattt tgaagcgggg gcagatcaaa gtattgtaca aggttttcaa
    10861 tatcagcaaa ctgtaacacg tgtagataat caaaatctta ctcaaattat tgaaaaatct
    10921 gttacaattg acaccaatat cgtattagtt gaaggcttta aaaatgctga ttttgaaaaa
    10981 gtcgtagtct atcgaaatga agaagagttg caagtattac aacaattgtc gaatgtttgt
    11041 tatagcatta atgtaaggga gcatgaagat tttacagcat ttgagcaatg gttattaaat
    11101 aaaattaaaa atgattgtga tacacaatta acatagagga ttgaaatgaa tgaaacaatt
    11161 tgaaatcgtg acagaaccga tacaaacaga acaatatcgt gaattcacta taaatgaata
    11221 tcaaggtgca gtagttgttt ttaccggtca tgttcgcgaa tggactaaag gcgtcaaaac
    11281 ggaatattta gaatatgaag cgtatattcc aatggctgaa aagaaattgg cacaaattgg
    11341 agatgaaata aatgaaaaat ggcctggaac gataacgagt attgttcata gaatagggcc
    11401 attacaaatt tcagatatcg ctgtattaat tgcggtttct tcaccgcatc gtaaagatgc
    11461 ctatcgagca aatgaatatg caattgagcg tataaaagaa attgttccga tttggaaaaa
    11521 agaaatttgg gaagatggtt caaaatggca agggcatcaa aaagggaatt atgaagaagc
    11581 gaagagggag gaataagaga gatgaaggta ctttacttcg cagaaattaa agatatatta
    11641 caaaaagcac aggaagatat tgtgcttgaa caagcattga ctgtacaaca atttgaagat
    11701 ttattgtttg aacgttatcc gcaaatcaat aataaaaagt ttcaagttgc tgtaaatgag
    11761 gaatttgtac aaaaatcgga tttcattcaa cctaatgata ctgttgcatt aattccaccg
    11821 gttagtggag gttaagggag catgaaagca ataattcttg caggtggtca ttcagtgcga
    11881 tttggtaagc ccaaagcttt tgcggaagtg aacggtgaga ccttttatag tagagtaatt
    11941 aagacattag aatcaacaaa tatgttcaat gaaattatta ttagtacaaa tgcgcaattg
    12001 gcaacgcaat ttaaatatcc aaatgttgtt atagatgatg agaatcataa tgataaaggt
    12061 ccattagcag gaatttatac aatcatgaag caacatcctg aagaagaatt gttttttgtc
    12121 gtttctgttg atacaccaat gattactggt aaagctgtaa gcacgttgta tcagttttta
    12181 gtttctcatc ttattgaaaa tcatttagat gtcgcagctt ttaaagaaga tggacgtttt
    12241 attccaacaa ttgcatttta tagtccgaat gcattaggcg ctataactaa agcactacat
    12301 tctgataatt acagttttaa aaatgtatat catgaattat caacggatta tttggatgta
    12361 agggatgtag atgcgccctc atattggtac aaaaatataa attatcagca tgatttggac
    12421 gctttaattc aaaaattgta agctgttagg aggtccacaa atggtagaac aaataaaaga
    12481 taaactagga cgtcccatcc gtgacttacg gttatctgtg acagatcggt gtaactttag
    12541 gtgtgattat tgcatgccta aagaggtatt tggagatgat ttcgtatttt tacctaaaaa
    12601 tgaactttta acgtttgatg aaatggctag aatcgctaag gtatatgcag aattaggtgt
    12661 aaaaaaaata cgcattacag gtggagaacc attgatgcga cgggatttag atgtacttat
    12721 agctaaatta aatcaaatcg atggtattga agatattggt ttgactacaa atggtttgtt
    12781 attaaaaaag catggacaaa agttatatga tgctgggctg cgcagaatta atgtcagttt
    12841 ggatgctatt gatgatacgc tatttcaatc aatcaataat cgtaatatta aagcgactac
    12901 gattttagaa caaattgatt acgcgacgtc tattggtttg aatgtaaaag taaatgttgt
    12961 tatacaaaaa ggtattaacg atgatcaaat cataccaatg cttgaatatt ttaaagataa
    13021 acatatagag attcgattta tagaatttat ggatgttggt aatgataatg gatgggattt
    13081 cagtaaagtt gtaactaaag atgaaatgct tacaatgata gagcagcact ttgaaatcga
    13141 tcctgtagaa ccaaaatatt ttggggaagt agcaaaatat tatcgccata aggataatgg
    13201 tgttcaattt ggtttgatta caagtgtttc acaatcattt tgttctacat gtacacgcgc
    13261 aaggctgtca tcagatggga agttttacgg atgtttattt gcaactgtcg atggatttaa
    13321 cgttaaagcg tttattcgtt ctggcgtgac cgacgaagaa ttaaaagaac aatttaaagc
    13381 tttatggcaa ataagagatg atcgatattc agatgagaga actgctcaaa cagttgccaa
    13441 tcgtcaacgt aaaaagataa acatgaatta tattggtggt taatgtgtag ggaccactac
    13501 atattaaatc attagagatg ttttaatatt tctgtcttac tccctaaaat acaatattat
    13561 ttattaaagt aaaaacggtc atatctatgc cagatttaat agaaatgatc gtttttaaag
    13621 tttttacaag ttggcggggc cccaacacag aagctgacag aaagtcagct tacaataatg
    13681 tgcaagttgg cggggcccca acatagagaa tttcaaaaag aaattctaca gacaatgcaa
    13741 gttggggaac ggggccccaa cacagaaggt gacgaaaagt cagcatacaa taatgtgcaa
    13801 gttggcgggg ccccaacata gagaatttca aaagaaattc tacagacaat gcaagttggg
    13861 gatcaacgaa ataaatttta tgagaatatc atttctatcc cactcttaag aatcactaca
    13921 taataaatct ttagtggttc tttaacattg atgtcacact ccatgccatt gagttgtaat
    13981 atatcttttt taggtataaa tgttgtcgaa taaacaacaa gttgtccaaa agatataaat
    14041 ctaaacaaga tatagccagc aatttaatat ttgtaataga taaaatgcta agtttgatat
    14101 ataataaatt taagtaattg tataataata tgaattacaa acatctaaga agaaacatag
    14161 gaggcatcat attatgagta ataaagttca acgttttata gaagcagaaa gggagttaag
    14221 tcagttaaag cactggttaa aaacaacaca taagatttca attgaagaat ttgtagtcct
    14281 ttttaaagtg tatgaagctg aaaagattag cggtaaagaa ttgagggata cattacattt
    14341 tgaaatgcta tgggatacaa gtaaaatcga tgtgattatc cgtaaaatct ataaaaaaga
    14401 gcttatttct aaattgcgtt ctgaaacgga tgaaagacaa gtattctatt tctatagtac
    14461 ttctcaaaag aaattgttag ataaaattac taaagaaata gaagtgttaa gcgttacaaa
    14521 ctaaaaactt aaaaagcatg ccaatcagta catcataatt gcgtcttggg gacagacaaa
    14581 tgatgaatag agattggcat gctttttatt tttgaatata aatatttagt tcatggcatt
    14641 tctagttaca tgacgtccat gaattaagaa gtaaacaagc atagtaatga ttgctaaagc
    14701 ggccataaag ccgaagattt cactatatga aaacatatga gtaaataacc caaggaatga
    14761 tggaccgaag ccgacacctg catctagacc aacgtaaaaa gtagatgtcg cgataccata
    14821 tttaatcggg ggtgagactt ttatcgcaat agattgcatt gcagatgata aatttccata
    14881 ccctaaacct aggcaagcac cagcaagtaa tattaaccag ctttgatagc ttgaaattaa
    14941 gcatacaaat gaaaggaaaa gcatgataaa tgctgggtag acaataatat tttcattttt
    15001 atcatccatc aatctaccag caataggtct agtaattaac gatgctatag catagcaaat
    15061 aaagaaatag cttgctgcag tgactaggtg tcgctctaaa gcaaatgctt gtaaataagt
    15121 taggatggac gcataggtaa cgccaattaa aagcataatt acagcaacag gaatggcctc
    15181 ttttgcaata aattgatgaa tactaaatct tggtttatca atgacattag tttcagtttt
    15241 gttatttgtt acttcgaaat caacttttat aaataatgag ataatgagtc cgagtatgcc
    15301 taatatgaca caaataataa acagtaagtc aattgcgtat tttgtaataa gtaacatgcc
    15361 tagaaatggg ccaatcgctg tacctaatac taaacttaag gaaaataaac tgatgccttc
    15421 actttttcta ttaacagggg taacgtatgc cgcaatagta cctgttgcag ttgtcacaac
    15481 tgcagttgcg ataccgttta tgagacgtac aaagattaaa aaagctaaag atccatcaat
    15541 aaaataaagt aattgcgtga taattaaagc aattaaacca ataaataata atcgtttagg
    15601 tccgatttga tttacaaatt tacctgtagc aaatcgacca attaaagacc cgacaataaa
    15661 tagtcctaca actaatccgg ctaatgaatc ggaagcgtcg aaagcttgct tactatagcc
    15721 agcaataact acaagtaaca aatacatgca taggtatacg ataaagttga ctaaaaagtt
    15781 aattgtaaaa cttttggtga aaataggtga tttatttaat gagccttgat tagtcattgt
    15841 gtgcctccct taataatatt tcatgaattt gattcatcac atttatggtt tcttgcattt
    15901 gatcttgcga aatgtttgtt ttggcaacta ctgcttgttg gagcttttgt acatcaagac
    15961 aaacaatctc atataattca tgccctttag gtgttaaagt taaaaacttt tgtcgtttat
    16021 cctctaaact gttttctact gttataaggt cattttctat tagagctttt aaagtttttc
    16081 ttgcagtagg cttttcaatt gcacgtctat gtgaaatttc aatgagagta gtgggctgat
    16141 gtttagcgat atcgcgtaag attaaccatt ggccataata tatattgtgt ttttctaaaa
    16201 tcggctcggc taattttaaa taggggcgat atattgttat aaaactattg aaaaattctt
    16261 gtgatagcat agtgacatct cctaagacaa aatagttagc ttagctaacc tttttacaac
    16321 aatagtaatt ataaaacggg agcaattaga aatcaatata taattattaa gagcaaaaat
    16381 aattatactt tgttaaaata agcgtaatta catgtaaata gggggatact aatgatattg
    16441 aaatttgatc acatcattca ttatatagat cagttagatc ggtttagttt tccaggagat
    16501 gttataaaat tacattcagg tgggtatcat cataaatatg gaacattcaa taaattaggt
    16561 tatatcaatg aaaattatat tgagctacta gatgtagaaa ataatgaaaa gttgaaaaag
    16621 atggcaaaaa cgatagaagg cggagtcgct tttgctactc aaattgttca agagaagtat
    16681 gagcaaggct ttaaaaatat ttgtttgcgt acaaatgata tagaggcagt taaaaataaa
    16741 ctacaaagtg agcaggttga agtagtaggg ccgattcaaa tggaaagaga tacacataaa
    16801 gatggtaagg taaagtggca attgctttat ataatgaatc aggatgatga tgaaattaag
    16861 ccaccatttt ttattcaatg ggaagaaagt gattccatgc gtactaaaaa attgcaaaaa
    16921 tattttcaaa aacaattttc aattgaaact gttattgtga aaagtaaaaa ccgatcacaa
    16981 acagtatcga attggttgaa atggtttgat atggacattg tagaagagaa tgaccattac
    17041 acagatttga ttttaaaaaa tgatgatatt tattttagaa ttgaagatgg taaagtttca
    17101 aaatatcatt cggttatcat aaaagacgca caagcaactt caccatattc aatttttatc
    17161 agaggtgcta tttatcgctt tgaaccatta gtataaatat acgtaagtgc tatgagcgag
    17221 aatgcccata tgaataatga caagcacaat ggaaagaatc gttaatatat tatttaatcg
    17281 tgatgactta attaaaatga aaaagattga taatataaat gtgaaaaaga taagtataac
    17341 ccgtaaacta aagtaattca cggtgagagg ttgactcaat gtcataatga ttgcaacgat
    17401 gttcataatt ataaatagac ttaaaataat tgttctcata tcaaacacct cattgttaga
    17461 ttattgacat tataacaggg gtaattgtat atgaacatta atgtggttgc ttgaggaaaa
    17521 atttattcat tgaagtcaag ttggttcatt ttagaaatga atatcgtgtt agatgatgaa
    17581 agtatattga agtataggta actagttgaa aagtattaat tgtacgataa cattaaattt
    17641 aacacgaaac atagatataa aatgattcac aattaaaatg ggtaaatttg aacttgctaa
    17701 actattaatt ggagcatgga catttcaaaa ataagagttc aaatcttaca caagctctga
    17761 atcgacacta taagatacaa actgtataat taaaggtatt gttaaataga aggagatatc
    17821 ataaatcatg gaaaagatgc atatcactaa tcaggaacat gacgcatttg ttaaatccca
    17881 cccaaatgga gatttattac aattaacgaa atgggcagaa acaaagaaat taactggatg
    17941 gtacgcgcga agaatcgctg taggtcgtga cggtgaagtt cagggtgttg cgcagttact
    18001 ttttaaaaaa gtacctaaat taccttatac gctatgttat atttcgcgtg gttttgttgt
    18061 tgattatagt aataaagaag cgttaaatgc attgttagac agtgcaaaag aaattgctaa
    18121 agctgagaaa gcgtatgcaa ttaaaatcga tcctgatgtt gaagttgata aaggtacaga
    18181 tgctttgcaa aatttgaaag cgcttggttt taaacataaa ggatttaaag aaggtttatc
    18241 aaaagactac atccaaccac gtatgactat gattacacca attgataaaa atgatgatga
    18301 gttattaaat agttttgaac gccgaaatcg ttcaaaagtg cgcttggctt taaagcgagg
    18361 tacgacagta gaacgatctg atagagaagg tttaaaaaca tttgctgagt taatgaaaat
    18421 cactggggaa cgcgatggct tcttaacgcg tgatattagt tactttgaaa atatttatga
    18481 tgcgttgcat gaagatggag atgctgaact atttttagta aagttggatc caaaagaaaa
    18541 tatagcgaaa gtaaatcaag aattgaatga acttcatgcc gaaattgcta aatggcagca
    18601 gaagatgaaa acatctgaaa agcaagctaa aaaagcgcaa aatatgatta atgatgcgca
    18661 aaataaaatt gctaaaaatg aagatttaaa acgagaccta gaagctttag aaaaggaaca
    18721 tcctgaaggt atttatcttt ctggtgcact attaatgttt gctggctcaa aatcatatta
    18781 cttatatggt gcgtcttcta atgaatttag agatttttta ccaaatcatc atatgcagta
    18841 tacgatgatg aagtatgcac gtgaacatgg tgcaacaact tacgatttcg gtggtacaga
    18901 taatgatcca gataaagact cagaacatta tggattatgg gcatttaaaa aagtgtgggg
    18961 aacatactta agtgaaaaga ttggtgaatt tgattatgta ttgaatcagc cattgtacca
    19021 attaattgag caagttaaac cgcgtttaac aaaagctaaa attaaaatat ctcgtaaatt
    19081 aaaacgaaaa tagattaacg actgaaatct gaacgctcat aagactgtca tttgcgttca
    19141 gattttttta cacaatatag aatggttgag taaaatattt ttgaatatag tgaaagaggg
    19201 ggaagtactg tgataaaaaa gctattacaa ttttctttag ggaataagtt tgctatcttt
    19261 ttaatggttg ttttagttgt cttgggcggt gtatatgcga gtgctaaatt gaaattagaa
    19321 ttactaccaa atgtacaaaa tccagttatt tcagttacaa caacaatgcc gggtgcaacg
    19381 ccacaaagta cccaagatga aataagtagt aaaattgaca atcaagtaag atcattggca
    19441 tatgtgaaaa atgttaaaac gcaatccata caaaatgctt caattgtaac agttgaatat
    19501 gaaaataata cagatatgga taaagcagaa gaacagctta aaaaagaaat cgataaaatt
    19561 aaatttaaag atgaagttgg tcaaccagaa ttaagacgta attcgatgga tgcttttccg
    19621 gttttagcat attcattttc aaataaagag aatgacttga aaaaagtaac gaaagtactg
    19681 aatgaacaat taataccaaa attgcaaacg gtagatggtg tgcaaaatgc gcaattaaat
    19741 gggcagacga accgtgaaat cacccttaaa tttaagcaaa atgaacttga aaaatatggg
    19801 ttgactgctg atgatgtaga aaactatcta aaaacggcaa caagaacaac gccacttgga
    19861 ttgttccaat ttggtgataa agataaatca attgttgttg atggtcaata tcaatctgtt
    19921 gatgctttta aaaacataaa tattccatta acgttggcag gaggacaagg gcaatctcaa
    19981 tcccaaagtg acaataaaca aaattcagcc atgtcagacg ttaatcaggc atcaccacag
    20041 caaaattcaa aagcgtcagc accaaataat ataagtggta tgccgacagc gaaactagga
    20101 gatttagctg atattacagt tggtgatgtg cgtacttcta tttctaaaac gaatggaaag
    20161 gatgcggtta atctacaaat aactaaagct caagatgcca atacagttca agtagccaaa
    20221 gatgtacaac gtaaaattga tacattcgtt gatgaaaata aagattttaa tgtcacaaaa
    20281 acaatggata ctgcaaagcc tgttgagaaa tcactttata cgatggttga aaaagcatca
    20341 ttaggtacaa tcgtggcaat tatagttatt ttgctgtttt taagaaacat tcgtacgacg
    20401 gcaatttcta ttatatcgat tccgttatca cttcttatgg cgcttattgc tctgaaattg
    20461 agtgatgttt cattgaatat actaacgtta ggtgcattaa cagtagcgat tggacgtgtg
    20521 atagacgatt cgattgtagt tgttgaaaat atttatcgac gcttaacaga ttcagaagaa
    20581 caactaaaag gtgaaaattt aattatcagt gcgacaactg aagtatttaa accaataatg
    20641 tcatcgacac tagttactat tatcgtcttc ttaccacttg tgtttgtatc aggttcagta
    20701 ggcgaaatgt ttagaccttt tgcattggct attgcattta gtttattagc atcgttatta
    20761 gtgtcaatta cactcgttcc agcgttggca gctacactat ttaaaaaagg cgttaaacgt
    20821 cgtaataaac aacatcaaga aggattaggt gttgttagta caacttataa aaaagtatta
    20881 cattggtcat taaatcataa gtggattgta attatattaa gtacattaat tttggttgca
    20941 actattgtat ttggaggacc gagactaggc actagcttta tttcagcagg tgacgataaa
    21001 tttttagcta ttacttatac accgaagcct ggtgaaacgg agcaagcagt gttgaatcat
    21061 gcgaaagatg ttgaaaaata tttaaaacag aaaaagcatg taaaaacaat tcaatactca
    21121 gttggcggta gtagtccagt agatccaacg ggtagtacaa atagtatggc aatcatggtt
    21181 gaatatgata atgacacgcc taattttgat gtagaagcgg ataaggttat taaacatgca
    21241 gatggcttta aacatcctgg agagtggaaa aatcaagatt taggaacagg tgcaggtaat
    21301 aaatctgtag aggttactgt aaaaggtcca tcaatggatg ccataaaatc aactgtaaaa
    21361 gatattgaac agaaaatgaa acaggttaaa ggactagcca atgtcaaatc tgatttatcg
    21421 caaacatatg atcagtatga aattaaagtc gatcaaaata aagcggcaga aaatggtatt
    21481 tctgcaagtc aacttgcaat gcacttgaat gaaaacttac cagaaaaaac agttacgact
    21541 gttaaagaaa atggtaaaac tgttgatgtt aaagtcaaac aaaataagca aacagactgg
    21601 tcagaagata agttgaataa tataactttg aaaaagccga ctggtggtac gattaaattg
    21661 ggagatatcg ctacgttagt taaaacaacg acaccaagta aattgacgca agaacaagga
    21721 gattatgcaa cgacggtatc tgctaaagta acaaataaag atgtgggtgg cacaacacga
    21781 caagtgatgt ctaaaataaa taatttggac aaaccgaata atgtaaaggt taatatcggt
    21841 ggtgcatcag atgatattaa caatgcaatg acacaattag cctttgcaat gttagctgca
    21901 atcattatcg tatatttaat cctagttatt acatttaaag gtggcctagc accatttaca
    21961 attttattct ctttaccatt tacagttatc ggtgtaatta ttgcactatt aatcacagga
    22021 gaaacaatat cagtaccaag tttaattggt atgctaatgt taattggaat cgtagtaaca
    22081 aatgccattg tgttaataga ccgtgttatt aataatgagc aacagggcat ggagatgaaa
    22141 gaagcattaa tcgaagcagg cggtactaga attagaccga tattaatgac ggcgattgca
    22201 acaattggtg cattagttcc tttgttattt ggtcaagata gctcgattct tatttcgaaa
    22261 ggattagctg ctacagttat tggtggttta atttcatcaa ctttattaac acttgtagtt
    22321 gtaccagtaa tatatgaaat tctctttact ttaaaaaaac gattcactaa acgatagata
    22381 atataaaagc cataaaagcg gtcatgatat tgactacttt tatggctttt taaaatttga
    22441 aattttagac cttatttcac gtgcgattta atcgatgtgt taataaaaca atactggcat
    22501 tcaacatgta atccgataat aaaataaggt tgcaattatg ttattgcata ttttgtttat
    22561 tacttagaat aatacgatgg ttaagatgaa atatatgaca aagataaata atgacattaa
    22621 attcaagatg tttacgcctt ttagatcttt tttaagcatt ttaaatcctg tcacttcgac
    22681 attaccgact aagagtaata agacgataaa tacccaccaa ttctcttttg gaaaactcat
    22741 cgtcgttaat gctaagacga ataataacaa cacacccata ataattctca aaatgcgagt
    22801 aaatacaact tccattttaa aaatactaat aaaactaaca attagataaa gtattgctag
    22861 taaaatcaga taaaattgca tatgataact tcctctccgt ttcaaagggt tcatgtagta
    22921 cagcagcgta gaaaacataa atagcagtgt tgggatagat atgcgtgtat gttgttaatg
    22981 aaacactaaa tcataacacg acaacatctt gtgattctag atgtaattga gcgaaaaatg
    23041 aagcaattca tcaatataac gtattcagag caatgctagt caaatttatg taacaaaaag
    23101 catttattgc ttgaatgata gattattaat cttgcatttg agcacgaacg tttctaattt
    23161 cattttcaat atctttaagt tgacgaacaa gtgcggaagt atctaaattc aaagattccc
    23221 gtttttctat gtcaccttga agacgaacat aatcatattt aagttcggct agttggtttt
    23281 ctaaagacat actataacac tcctttgaaa tataattaag tgcatttaat gtagattata
    23341 gcatgaaaca gtagacttct aaaatatctg tggagtatgt taaagttata aattatgaag
    23401 attattgaaa gaagggataa cagtgacaga acaatttact atgattattt tattaattgc
    23461 gcttggttac tttttgaagc gcattaattt tataaaagca acagatagtc aagtcattgc
    23521 tacattagtt cttaatgtca cactcccatc tttagttatt gtgaatttaa acagtgcaga
    23581 attaaaattg tcattctcca ttttaccaat tttaatgatt atatatggta ttgtggcgaa
    23641 aatgattgct gtctggtttt ttagaaaata tgataatcac atgcgtggat cagtaggtat
    23701 gatgactggt gctatgaata ttggattgtt tgcatatcca ctaattgaag tgatatggcc
    23761 taaaacggga cttatttatt ttgttatggc tgatataggt ggtgcctttg tgatgtttgg
    23821 aattacttac tttgtaggta gctactttag tgaaggtagt gatcaattcg attttaaata
    23881 tttaggtaag aaattattac aatcagtgcc actcgttaca tacattgtaa tgtttatatt
    23941 gaatataagt cacatccatt taccacatgt ggcaatcgat ttctttagca tactttccca
    24001 tgcaaatatg ccgttatcaa tgattttgct aggtgtcatg ttgaatttta ctctggaaag
    24061 aaagtatata cctgctacta ttaaatattt atgtttgcat tatggattag cattagttgc
    24121 gggattgtta gtatatgtgt tgttaccagt atcagatgat atgattaaga cgacattgtt
    24181 ggtcacttgg atgttcccgg taggtgttgc aattatttct tatggtattc agtttaaata
    24241 tcgaacactg acatttatag gtatgacgac caatttgaca atcattatta gtatcattat
    24301 tttgtacgtg tatcaggcag tgtttgtcta gtttacacat ttgaaaataa aatccatttt
    24361 agtattcgta gaaaatgaga gcccccttta ccacaatatg taaatgtata ttgtgaaaag
    24421 ggggctttta tttatgacat agttgttcaa ctcatacatt caagttgata atgcagtgcg
    24481 ttatgcagct atccacttca tatgtatggc gcatacttga tatatgaata tcatcaagat
    24541 tttaaaagta tcgctaattt taaagtctta aaacgcatga ttaatgtcat gatgtgttat
    24601 tgcgttttgt ttttagagcc acgtatttcc taggacatat agaggaacat tcaacgtaaa
    24661 attcccgaaa attaatttta atcattctat aagtgaatgg ttattttcag aagtgattac
    24721 atttttacgt tacatgagta tttaactttc tatttcaaat tacctagaat tatagcagct
    24781 atcacgataa taataatacc tgcccaaata cccgtcatct gacgacgatc ttttctttct
    24841 cctaaaatga aaataccgcc taaggttgaa acgataacaa gtaactgtga taatgagaaa
    24901 cttgtagcta caccaacttt tggttgagaa tagaacatga acaagttacc aattccccaa
    24961 atcacacctg gcaatagatt aagtgctgtt gatttaagtg atgttttatg attcatggat
    25021 aggataaagc caccaattgc cataccgaca gattggaaga acaatgcatc agttccacca
    25081 acaccaaaga tgtcaccaag tacaacgaaa cctacatatc caactgtaga tacaattaaa
    25141 atacccattg cttttttaaa ttcaggatta tctgattgac gttcattttt agctttaagt
    25201 gaagtaagtg ctacaccagt aactaataat atcatggcga ttaaaccaaa gataatttga
    25261 gtcattgaag accattcacc taaaaagata acgctgaata atgttgtacc aactaattgc
    25321 atacctgtag aaactggcat agtatttgat acaccgatta aactaataga tttaagctga
    25381 ttcgcttgtc caaacgccca taatgcacca gaaataagac cgacaataat tacagtagga
    25441 ttattgaatt ttgcgaaacc agtaataagt aaacctaatc caacaatgag tgcgcctagc
    25501 gtcgtaccac gaatttggtt gtaaggtcca ccgccgacga acacattaat aagaacgaca
    25561 cttccccaga ataaagcagg taaaagtgcg attaagaaat caagaaattg caagactact
    25621 cactctccta tactatttat gcaagtgctc cccttaattg acacttgcat gttattgtaa
    25681 agcattctgc cctaatagta aaaaacaact acttataatt ttcaatattt acaaaaagta
    25741 gaaaaaccca caatacatgc taatgatgta ctgtagggta tttgcttggt gttttattca
    25801 gggattgttt gatcttttac gttacgtgat tcgaatttga atttaatgta ctcatagttg
    25861 atatggaaac ctagaccatt aaatcgttcg atgagtttat tatttttatt acgaatttta
    25921 aaataaactt ttctaatatc aaaattatca aaagcatatt gcaatgcata cgcaagcaac
    25981 tcgaaagcga tacctttcaa acggtaatct acatgagaac tgaagtattt aatctcggcg
    26041 attgactgtt gtgaatcaat ttcaaggtat aaataacctt taagtaaacc ttcgctaaca
    26101 aataaaaaca aatgatgatg gtcgtctaaa ctatcaatga tatcttgtgg tgactgtgcc
    26161 tgatatttaa atgtagttgt gtgtaatttg ctgaaagcac gataaaaccc tttgtgataa
    26221 ggaatgatat ttggttggtg catatcttct tctaatcttg tacgggcttc tatgtaatag
    26281 tcagtgaagt tataacttgc ttgaataact ttcattaatg gtttgtattg ttgaatgcct
    26341 tcttcaaaag aaaagttaaa gacggcatcg tcaggttggt tcgaagtcat tgctttaaat
    26401 agcgtttcaa acgtatcgac agataataca taattgtcag ccacgaaagg gcctatgact
    26461 ttgaatttat ttttatcgta tgcaaaagac aatatgattg ctttgattga atcgttttct
    26521 ttaatcgaaa atacaccagg tgattcaatc gcatcggtta ttaatacatc tatttcattg
    26581 tactgttgag gaagtttata taagtatgat gctgattcat aattcgaatg ttcaatgaac
    26641 gattttattt gtgtaatatc atataactga actatgctca tgtcattacc tccgtacttt
    26701 ttgttacttt tattatatag tatttcaact gaaatgaaag ttaatagtga tattaacatg
    26761 ttacaataca tttaacacat catttgcaat cattgaattt aaatcaaaga ttagtggaat
    26821 agatgaagca cgttcgaaat aaaagaacgt atgagaaagg ataatttatg aaatctttaa
    26881 tattagctga aaaaccatca gtcgcaagag atattgctga tgctttacaa ataaatcaga
    26941 agcgtaatgg ttactttgaa aataaccaat atattgtcac gtgggcgtta ggtcatctag
    27001 tgacaaatgc gacacctgaa caatacgata aaaatttaaa ggaatggcga ttagaagacc
    27061 ttccaattat acctaaatat atgaaaactg ttgttattgg taaaacaagc aaacaattta
    27121 aaacagtaaa agcgttaatt ttagataata aagtgaaaga tattattatt gcaacagatg
    27181 ctggacgaga aggtgaacta gttgcaagat tgattttgga taaagttggt aacaaaaagc
    27241 caatccgtcg attatggatt agctcagtta ctaaaaaagc tattcaacaa ggttttaaaa
    27301 atttaaaaga cggtcgtcaa tataacgatt tgtattatgc agcgttagcg agaagtgagg
    27361 cagattggat tgttgggatt aatgcaacgc gtgcactaac aacaaagtat gatgcacagc
    27421 tatccctggg acgtgttcag acaccaacga ttcaattagt aaatacacga caacaagaga
    27481 ttaatcagtt caaaccacaa caatacttta cattatcatt aacggtaaaa gggtttgatt
    27541 ttcagctaga atcaaatcag cgatatacca ataaagaaac tttagaacag atggttaata
    27601 atttgaaaaa tgtcgatggt aagattaaat ctgttgctac taaacataag aagtcgtatc
    27661 cgcaatcact gtacaattta acagatttac aacaagatat gtatagacgt tataaaattg
    27721 gacctaaaga aacattgaat acacttcaaa gcttatatga gagacataaa gtcgtaacct
    27781 atccaagaac agattcaaac tatttaacaa ctgatatggt agatactatg aaagaacgta
    27841 ttcaggcgac gatggcaaca acatataaag accaagcacg cccattaatg tctaaaacat
    27901 tttcatcaaa aatgtcgata tttaataatc aaaaagtatc tgatcaccat gcaattattc
    27961 ctacagaagt gagacctgtc atgtcagact taagtaatag agaattaaag ttatacgata
    28021 tgattgtcga gcgtttttta gaagctttaa tgcctccgca cgagtatgac gcgataactg
    28081 taactttaga ggttgcaggg cacacatttg ttttgaaaga gaatgtaaca actgttttag
    28141 gttttaaatc tattagacaa ggtgaatcta ttacagagat gcaacagcct ttttcagaag
    28201 gcgatgaagt gaagatttca aaaacaaata ttagagaaca tgaaacaaca cctccagaat
    28261 attttaatga aggttcgtta ttaaaagcga tggagaaccc tcagaacttt attcaattga
    28321 aggataaaaa atatgcgcaa actttaaaac aaacaggtgg tatcggcaca gttgcaacaa
    28381 gggccgacat tatcgataaa ttatttaata tgaatgccat tgaatcaaga gacggtaaaa
    28441 ttaaagtaac gtcaaaaggt aaacaaatat tagaattagc accagaagaa ttaacgtcgc
    28501 cacttttaac tgcacaatgg gaagaaaaat tacttttaat tgaacgtggt aaatatcagg
    28561 cgaaaacatt tattaatgaa atgaaagatt ttacgaaaga tgttgtaaat gggattaaaa
    28621 atagtgatcg taaatataaa cacgataatt taacaaccac agaatgccca acgtgtggta
    28681 aattcatgat taaagttaaa actaaaaatg gtcagatgct tgtgtgccaa gatccatctt
    28741 gtaagacgaa aaagaatgta cagcgcaaaa caaatgcaag atgtccaaac tgtaaaaaga
    28801 aattaacgtt gtttggtaaa gggaaagaag cggtatatcg ttgtgtttgt ggacattctg
    28861 aaacgcaagc acatatggat cagcgtatga agtctaaatc ctctggtaaa gtatctcgta
    28921 aagaaatgaa aaagtatatg aataaaaatg aaggtttaga caataatccg tttaaagatg
    28981 cattaaagaa cttgaattta tagataaaat cgaacaaagt tgaatcagaa aaacgaaaag
    29041 ttcgcttttg gtattgtttt ttattaagaa tgatattaaa ctattaaggt attttaaaaa
    29101 aaggagcatc cattcgtgaa aaactatttc cagttcgata aatatggaac aaactttaaa
    29161 agagaaatct taggcggtat cacaactttc ttatctatgg cctatatttt agcagttaac
    29221 ccgcaagttt taagtttagc aggtgttaaa ggcgtatcag aagatatgaa aatggaccaa
    29281 ggtgccattt ttgtagcgac tgcattagca gcatttgtag gctcgctatt catggggcta
    29341 atagctaaat atccaattgc gttagcacca ggtatgggat taaatgcatt ctttgcattt
    29401 actgttgtgt taaccatggg tattccttgg caagttggtt taacaggcgt tttattctca
    29461 ggtatattct ttgcgatatt aaccgtcaca gggtttaggg aagttattat taatgcaatt
    29521 ccttatcaaa tgaagatggc tgtttcagca ggtataggat tattcattac atttgtaggt
    29581 ttacaaagtt cgggtattat agttaaaaat gaatctacat tagtaacatt gggacattta
    29641 actgatccac cagtactttt ggcgatattc ggtatcgtta ttacagttat tttatatgca
    29701 attaaattgc cgggttctat ctttatcggt atgatcatca cagcaatcgt tggtatgttc
    29761 actggattga ttcaaatgcc atctggtatt gtgggtaaga ttccaagtat tgaaccaacg
    29821 ttcggtgctg catttgaagc gtttaaagac ccaagtcaat tattaacgat acagttctta
    29881 attgttattt taacgttctt atttattgat ttctttgata cagctggtac attagtagct
    29941 gttgctactc aagcaggtat tatgaaagat aataaattac caagagctgg tagagcattg
    30001 ttctcagatt ctttagctac gattgtagga tcagtttttg gaacaactac aacaacttct
    30061 tatatcgaat caacttcagg tgttgctgta ggtgctagaa caggatttgc tagtatcgta
    30121 acaggttgtt gtttcctatt agcgctgttc tttagtccgt taatcgctgt tgtaacaagt
    30181 gcagttacga caccagcctt agtggtggta ggtgtattaa tggctgcaaa ctttgctgag
    30241 ataaactgga aaagttttga agttgcagtg ccagccttca ttacaatcat tatgatgcct
    30301 ttatcatatt caattgcaac tggtattgcg tgtggattta ttttctatcc aattacaatg
    30361 cttatctcta aaaaacataa agaagtacat ccgataatgt atgtattaat ggtactattc
    30421 attctgtact tcatatttgt tcatggttaa atccagacac aaaaaacgac tgccaatgat
    30481 tggtagtcgt ttttttcttt gaagtaagga taaaataaga agcgaaataa cataaagcga
    30541 tattatgaat gtctttaaat atccgatata aatatacttc ttttaaaatt gttggacgtg
    30601 tttcttctat attatatacc taattaaaaa ggttttaaga attattctct atgtccatct
    30661 ttgtattgtg tgtaaatgac acgagcattt ttattaggta tataggttgg cgctgataat
    30721 acaactagga caaatagaat cattaggaaa aataaaatcc aatgaatcaa aattcctaag
    30781 aaggggataa tagctaaaaa actacctact ataccaaata ttggaataat cgctataggt
    30841 ctaatcgcat tttgtctatt cactaataat ataatagtaa gaattagata aagtaatgca
    30901 ttaaagagta aaggctgcca tccaaaactt aaaataaacg ttgcacctaa aaaaggaatg
    30961 ccatagaaaa actcacaaag tattaaaaag atacctataa gtgctaaaga taattttggt
    31021 ccatttttca tattcattca ccaccgttct tatgactaat tatatagaat aattttcaat
    31081 gataataata aaatgattca aggtacgaat ttacaataaa gaaggaaata aaaaatatct
    31141 cgaaaatagt tgaactgact aagtatataa agtaaaatat aaaagtatgt gttagacaga
    31201 tagaaaaatt atgtgaaaag ccttgattta tcgatgtttt tcgtgtaata taactaacgt
    31261 tggactttaa gaaaagcgat gaagcgaaag gttactgaca cacccggccg ctttgccatg
    31321 gcgctgtgta agatagtttt cgtggagaag tctatcacta aatgtagacg aataaggagg
    31381 gaaaattatg gcaaaacaaa aaatcagaat cagattaaaa gcttatgatc accgcgtaat
    31441 tgatcaatca gcagagaaga ttgtagaaac agcgaaacgt tctggtgcag atgtttctgg
    31501 accaattccg ttaccaactg agaaatcagt ttacacaatc atccgtgccg tgcataagta
    31561 taaagattca cgtgaacaat tcgaacaacg tacacacaaa cgtttaatcg atattgtaaa
    31621 cccaacacca aaaacagttg acgctttaat gggcttaaac ttaccatctg gtgtagacat
    31681 cgaaatcaaa ttataataga caattttagg aggtggactt tcgatgacca aaggaatctt
    31741 aggaagaaaa attgggatga cacaagtatt cggagaaaac ggtgaattaa tccctgtaac
    31801 agtagtagaa gctaaagaaa atgttgtatt acaaaagaaa actgtagaag ttgatggata
    31861 caacgcaatc caagttggat ttgaagacaa aaaagcatac aaaaaagatg caaaatctaa
    31921 taaatatgct aataaaccag ctgaaggtca cgctaaaaaa gctgacgcag cacctaagcg
    31981 cttcattcgt gaattccgca atgtagacgt ggatgcttac gaagtaggtc aagaagtctc
    32041 agtagatact tttgtagctg gcgacgttat tgacgtaaca ggcgtatcaa aaggtaaagg
    32101 tttccaaggt gcaattaaac gccacggaca atctcgtgga cctatgtcac acggttctca
    32161 tttccacaga gcaccaggtt ctgtaggtat ggcttcagat gcttctagag tatttaaagg
    32221 ccaaaaaatg ccaggacgta tgggtggaaa cactgtaact gttcaaaact tagaagtagt
    32281 tcaagttgac acagaaaaca aagttatctt agtaaaaggt aacgtacctg gacctaaaaa
    32341 aggtttagta gaaatcagaa cttcaattaa aaaaggtaat aaataataag aagtgaaagg
    32401 aggaaatgca taatggctaa ttatgatgtt ttaaaattag acggaactaa atcaggttca
    32461 atcgaattaa gcgatgcagt attcggtatt gagccaaata atagcgtttt attcgaagct
    32521 attaatttac aacgtgcttc attacgtcaa ggtacgcatg ctgttaagaa tcgttcagca
    32581 gtaagcggtg gcggacgtaa accatggaag caaaaaggaa caggtcgtgc tcgtcaaggt
    32641 acaatccgtg ctccacaatg gcgtggcggt ggtatcgtat tcggaccaac tccaagaagt
    32701 tatgcataca aaatgcctaa gaaaatgcgt cgtttagctt tacgctcagc attatctttc
    32761 aaagctcaag agaatggctt aactgtagtt gacgcattca acttcgaagc tccaaaaact
    32821 aaagaattca aaaatgtatt atctacatta gaacaaccta aaaaagtatt agtagttact
    32881 gaaaacgaag atgtaaatgt tgaattatca gcacgcaaca tccctggcgt tcaagtgaca
    32941 actgctcaag gtttaaatgt tttagatatc actaatgctg acagcttagt aattactgaa
    33001 gctgctgcta aaaaagttga ggaggtgctc ggataatgga agcaagagat attcttaagc
    33061 gccccgtaat cactgagaaa tcttctgaag caatggctga agacaaatac actttcgacg
    33121 ttgatactcg tgttaacaaa acacaagtaa aaatggcagt tgaagaaatc ttcaacgtaa
    33181 aagttgcaag tgttaatatc atgaattaca aacctaagaa aaaacgtatg ggccgttacc
    33241 aaggctatac aaacaaaaga agaaaagcga ttgtaactct taaagaagga tcaatcgact
    33301 tatttaacta ataaaacaat taccattaag gaggtaagcg acaatggcta ttaaaaagta
    33361 taagccaata acaaatggtc gtcgtaatat gacttcgtta gatttcgcag aaatcacgaa
    33421 aactacacct gaaaagtcat tattaaaacc gctaccgaaa aaagcgggac gtaacaacca
    33481 aggtaaattg actgtaagac accatggtgg tggacacaaa cgtcaatacc gtgttatcga
    33541 tttcaaacgt aacaaagatg gtatcaatgc aaaagttgat tctattcaat atgatccaaa
    33601 ccgctcagca aacatcgctt tagttgtata tgcagacggt gaaaaacgat atatcattgc
    33661 tcctaaagga ttagaagtag gtcaaatcgt tgaaagtggt gctgaagctg acatcaaagt
    33721 tggtaacgca ttaccattac aaaacattcc agttggtaca gtagtacaca acatcgagct
    33781 taaacctggt aaaggtggac aaatcgctcg ttcagctggt gcaagtgctc aagtacttgg
    33841 taaagaaggt aaatacgtat taatcagatt aagatctggt gaagttcgta tgatcttatc
    33901 tacttgccgt gctacaatcg gtcaagttgg taacctacaa cacgaattag ttaacgttgg
    33961 taaagccgga cgttcaagat ggaaaggtat ccgtccaaca gttcgtggtt ctgtaatgaa
    34021 ccctaacgat cacccacacg gtggtggtga aggtcgtgct cctatcggta gaccatctcc
    34081 aatgtcacca tggggtaaac ctacgcttgg taagaaaact cgtcgtggta aaaaatcatc
    34141 agacaaactt atcgttcgtg gacgtaagaa aaaataatat aatcaactta tttgggtgtg
    34201 cggcttaaag ctgcacgcac ataataagaa gggaggcgcc caaatggctc gtagtattaa
    34261 aaaaggacct ttcgtcgatg agcatttaat gaaaaaagtt gaagctcaag aaggaagcga
    34321 aaagaaacaa gtaatcaaaa catggtcacg tcgttctaca attttcccta atttcatcgg
    34381 acatactttt gcagtatacg acggacgtaa acacgtacct gtatatgtaa ctgaagatat
    34441 ggtaggtcat aaattaggtg agtttgctcc tactcgtaca ttcaaaggac acgttgcaga
    34501 cgacaagaaa acaagaagat aatatctatt aagtagagga ggacatccta atggaagcaa
    34561 aagcggttgc tagaacaata agaatcgcac ctcgtaaagt aagactagtt cttgacttaa
    34621 tcagaggtaa aaatgctgct gaagctattg caattttaaa attaacaaac aaagcttcat
    34681 caccagtaat tgaaaaagta ttaatgtccg ctttagctaa tgctgaacat aactatgaca
    34741 tgaacacaga tgaattagta gttaaagaag catatgctaa cgaaggacca acattaaaac
    34801 gtttccgtcc acgtgcgcaa ggtcgtgcaa gtgcgattaa caaacgtaca agccacatta
    34861 caatcgtcgt aagtgacggt aaagaagaag ctaaagaagc ttaattaact tttaaggagg
    34921 gaatactgtg ggtcaaaaaa ttaatccaat cggacttcgt gttggtatta tccgtgattg
    34981 ggaagctaaa tggtatgctg aaaaagactt cgcttcactt ttacacgaag atttaaaaat
    35041 ccgtaaattt attgataatg aattaaaaga agcatcagtt tctcacgtag agattgaacg
    35101 tgctgcaaac cgtatcaaca ttgcaattca tactggtaaa cctggtatgg taattggtaa
    35161 aggcggttca gaaatcgaaa aattacgcaa caaattaaat gcgttaactg ataaaaaagt
    35221 acacatcaac gtaattgaaa tcaaaaaagt tgatcttgac gctcgtttag tagctgaaaa
    35281 catcgcacgt caattagaaa accgtgcttc attccgtcgt gtacaaaaac aagcaatcac
    35341 tagagctatg aaacttggtg ctaaaggtat caaaactcaa gtatctggtc gtttaggcgg
    35401 agctgacatc gctcgtgctg aacaatattc agaaggaact gttccacttc atacgttacg
    35461 tgctgacatc gattatgcac acgctgaagc tgacactact tacggtaaat taggcgttaa
    35521 agtatggatt tatcgtggag aagttcttcc tactaagaac actagtggag gaggaaaata
    35581 ataatgttac taccaaaacg tgtaaaatat cgtcgtcaac atcgtcctaa aacaactggt
    35641 cgttctaaag gcggtaacta cgtaacattt ggtgagtttg gtttacaagc tacaacaacg
    35701 tcttggatca catctcgtca aatcgaatct gctcgtatag caatgacacg ttacatgaaa
    35761 cgtggcggga aagtttggat taaaatcttc ccacatacac catatactaa aaaaccttta
    35821 gaagtacgta tgggtgctgg taaaggtgcg gttgaaggct ggatcgcagt tgttaaacca
    35881 ggtagaattt tattcgaagt tgctggcgtt tctgaagaag ttgcgcgtga agcactacgt
    35941 ttagcaagtc acaaacttcc agtaaaaact aagtttgtaa aacgtgagga attgggtggt
    36001 gaaacaaatg aaagctaagg aaattagaga cttaaccact tcagaaatcg aagaacaaat
    36061 caaatcttca aaagaagagc tttttaacct acgctttcag ttagctacag gtcaattaga
    36121 agaaactgca cgtattcgta cagtaagaaa aacgattgca cgtctaaaaa ctgttgctcg
    36181 tgaaagagaa attgaacaaa gtaaggctaa tcaataatta agtaagagga ggttacaaaa
    36241 gtgagcgaaa gaaacgatcg taaagtttat gtaggtaaag ttgtttcaga caaaatggac
    36301 aagactatta cagtacttgt tgaaacttac aaaacacaca aattatacgg taaacgagta
    36361 aaatactcta aaaaatacaa aactcatgat gaaaacaatt cagctaaatt aggagacatt
    36421 gttaaaattc aagaaactcg tcctttatca gcaacaaaac gttttcgttt agtagagatt
    36481 gttgaagagt cagtaattat ttaatacaag tttagagata agggaggttt aactaatgat
    36541 ccaacaagaa acacgcttga aagtagcaga caactctggt gctcgtgaag ttcttacaat
    36601 caaagtatta ggtggatctg gtcgtaaaac agcaaacatc ggcgatgtta tcgtatgtac
    36661 tgttaaaaat gcaacaccag gtggcgttgt taaaaaaggt gacgttgtca aagctgtaat
    36721 cgtacgtact aagtcaggtg ttcgtcgtaa tgacggttca tacatcaaat ttgatgaaaa
    36781 tgcatgtgtt atcatccgtg atgacaaagg cccacgtggt actcgtatct tcggacctgt
    36841 tgctcgtgaa ttacgtgaag gtaacttcat gaaaatcgta tcattagcac cagaagtact
    36901 ttaattaata acaaccaata caaataagga ggtgcccaca tgcatatcaa aaaaggtgac
    36961 aacgttaaag ttatcgcagg taaagacaaa ggtaaagaag gtaaagtaat tgctactcta
    37021 cctaaaaaag accgtgtcgt tgtggaaggt gttaacatta tgaaaaaaca ccaaaaacca
    37081 actcaattaa atcctgaagg tggaatctta gaaacagagg cagcaatcca tgtttctaat
    37141 gtacaattat tggaccctaa aacaaacgaa ccaactcgtg taggttacaa atttgttgat
    37201 ggtaaaaaag ttcgtatcgc taaaaaatct ggcgaagaaa ttaaatctaa taattaataa
    37261 caagttgaaa ggaggatccc actttgaacc gtttaaaaga aaagtttaac actgaagtta
    37321 ctgaaaactt aatgaaaaaa ttcaattata gttcagtaat ggaagtacca aaaatagata
    37381 aaatcgttgt gaacatgggt gtaggtgacg cagtacaaaa ttctaaagta ttagacaatg
    37441 ctgttgaaga attagaattg atcactggtc aaaaaccatt agtaactaaa gctaaaaaat
    37501 caatcgcgac tttccgttta cgtgaaggta tgccaatcgg tgcgaaagta acacttcgcg
    37561 gtgaaagaat gtatgaattc ttagacaaat taatttcagt atcattacca cgtgtacgtg
    37621 acttccaagg tgtttctaaa aaagcatttg acggacgcgg taactacact ttaggtgtta
    37681 aagaacaatt aattttccca gaaatcgact atgataaagt aagtaaagtt agaggaatgg
    37741 atattgttat cgtaacgact gctaacactg atgaagaagc tcgtgaattg ttagctaact
    37801 tcggtatgcc attccgtaaa taattattta aaggaggcta attaagtggc taaaacttca
    37861 atggttgcta agcaacaaaa aaaacaaaaa tatgcagttc gtgaatacac tcgttgtgaa
    37921 cgttgtggcc gtccacattc tgtatatcgt aaatttaaat tatgccgtat ttgtttccgt
    37981 gaattagctt acaaaggcca aatccctggc gttcgtaaag ctagctggta ataaaaaaga
    38041 gtctgaaagg aggcaacaat caatgacaat gacagatcca atcgcagata tgcttactcg
    38101 tgtaagaaac gcaaacatgg tgcgtcacga gaagttagaa ttacctgcat caaatattaa
    38161 aaaagaaatt gctgaaatct taaagagtga aggtttcatt aaaaatgttg aatacgtaga
    38221 agatgataaa caaggtgtac ttcgtttatt cttaaaatat ggtcaaaacg atgagcgtgt
    38281 tatcacagga ttaaaacgta tttcaaaacc aggtttacgt gtttatgcaa aagctagcga
    38341 aatgcctaaa gtattaaatg gtttaggtat tgcattagta tcaacttctg aaggtgtaat
    38401 cactgacaaa gaagcaagaa aacgtaatgt tggtggagaa attatcgcat acgtttggta
    38461 ataaaaaata aggaggtgcc ataacatgag tcgtgttggt aagaaaatta ttgacatccc
    38521 tagtgacgta acagtaactt ttgatggaaa tcatgtaact gttaaaggtc ctaaaggtga
    38581 attatcaaga actttaaatg aaagaatgac attcaaacaa gaagaaaaca caattgaagt
    38641 tgtaagacca tctgattcta aagaagatag aacaaaccat ggtacaactc gtgctttatt
    38701 aaacaatatg gtacaaggtg tttctcaagg atacgtaaaa gtacttgaac ttgttggtgt
    38761 aggttaccgt gctcaaatgc aaggtaaaga cttaatcctt aacgttggtt attctcaccc
    38821 agtagaaatt aaagctgaag aaaacattac tttctcagtt gagaaaaaca cagtcgttaa
    38881 agttgaaggt atttcaaaag aacaagttgg agcattagca tctaacatcc gttcagtaag
    38941 acctccagag ccttacaaag gtaaaggtat tcgttaccaa ggtgaatacg ttcgccgtaa
    39001 agaaggtaaa actggtaaat aatagataaa ctctaaagaa aggagtattg aaatgatcag
    39061 taaaattgat aaaaataaag tgcgtttaaa aagacatgct cgtgttcgta ctaacttatc
    39121 aggtacagct gaaaagccac gtttaaacgt atatcgttca aacaagcata tctacgctca
    39181 aattattgat gataataaag gcgtaacatt agctcaagct tcttcaaaag acagcgacat
    39241 tgctactaca gcaactaaag ttgaattagc aactaaagtc ggtgaagcaa ttgctaaaaa
    39301 agctgctgac aaaggcatta aagaaatcgt atttgaccgt ggaggatatt tatatcacgg
    39361 acgtgttaaa gcattagctg aagcagcaag agaaagcgga ttagaatttt aatttaaagg
    39421 agggacaaat acatggctcg tagagaagaa gagacgaaag aatttgaaga acgcgttgtt
    39481 acaatcaacc gtgtagcaaa agttgtaaaa ggtggtcgtc gtttccgttt cactgcatta
    39541 gttgtagttg gagacaaaaa tggtcgtgta ggtttcggta ctggtaaagc tcaagaggta
    39601 ccagaagcaa tcaaaaaagc tgttgaagca gctaaaaaag atttagtagt tgttccacgt
    39661 gttgaaggta caactccaca cacaattact ggccgttacg gttcaggaag cgtatttatg
    39721 aaaccggctg cacctggtac aggagttatc gctggtggtc ctgttcgtgc cgtacttgaa
    39781 ttagcaggta tcactgatat cttaagtaaa tcattaggat caaacacacc aatcaacatg
    39841 gttcgtgcta caatcgatgg tttacaaaac cttaaaaatg ctgaagatgt tgcgaaatta
    39901 cgtggcaaaa cagtagaaga attatacaat taaggaggga aaactagtta tggctaaatt
    39961 acaaattacc ctcactcgta gtgttattgg tcgtcctgaa acacaacgta aaactgttga
    40021 agctttaggt cttaaaaaga ctaacagttc agtagttgtt gaagataacc ctgctattcg
    40081 tgggcaaatc aacaaagtta agcacttagt aacagtagaa gaaaaataat caaggaggtg
    40141 ccgaaatgaa attacatgag ttaaaaccgg cagaaggttc acgtaaagaa cgcaatcgtg
    40201 ttggacgtgg tgttgcgaca ggtaacggta aaacaagtgg tcgcggacac aaaggtcaaa
    40261 aagctcgttc aggcggtggt gtaagaccag gatttgaagg tggtcaatta ccattattcc
    40321 gtcgtttacc aaaacgtggt tttactaaca taaatcgtaa agaatatgct attgttaact
    40381 tagaccaact taataaattt gaagatggta ctgaagtaac tccagcttta ttagtagaat
    40441 ctggtgttgt taagaatgaa aaatctggta tcaaaatact aggtaatggt tcacttgata
    40501 agaaattgac agtgaaagct cataaattct cagcttcagc agcagaagct attgatgcta
    40561 aaggtggagc acacgaggtg atctaatgat tcaaaccctt gtgaacttct ttagaacaaa
    40621 agaggttcgt aacaagattt tcttcacact agcaatgtta gtaattttta aaatagggac
    40681 ttatatacca gctccaggag taaatcctgc agcttttgat aatccccaag gttctcaagg
    40741 tgccactgag ttattaaata cttttggtgg cggagccttg aaacgatttt ctatttttgc
    40801 aatgggtatt gtaccctaca tcactgcatc aatcgtaatg caattattac aaatggatat
    40861 tgtccctaaa ttctcagaat gggcaaaaca aggtgaagta ggtagaagaa agttaaataa
    40921 cgttactcgt tatttagcaa tttctttagc atttatccaa tctataggta tggcattcca
    40981 atttaataat tatctcaaag gtgcgctgat tatcaatcag tcaattatga gttatttatt
    41041 aatagcacta gttttgacag caggaactgc tttcttaata tggcttggtg atcaaatcac
    41101 tcagttcggt gttggtaatg gtatttctat tatcatattt gcgggtattt tatcaacgtt
    41161 gccagccagt ctaattcaat ttggacaaac tgcgttcgtt gggcaagaag atacatcatt
    41221 agcatggttg aaagtattag gacttttagt gtcactgata ctattaactg taggtgctat
    41281 ttatgtactt gaagccgttc gtaaaattcc gattcaatat gctaagaaac aaactgctca
    41341 aagattaggt tcacaggcaa cttatctacc tttaaaagtg aactcagccg gtgttattcc
    41401 tgtaatcttt gcaatggcgt tcttcttact gcctagaaca ttaacgttgt tctatccaga
    41461 taaagaatgg gcacaaaaca ttgcgaatgc tgcaaatcct tcaagtaatg ttggtatggt
    41521 tgtatatatt gtactaatta tactattcac atacttctat gcatttgtac aagttaatcc
    41581 ggaaaaaatg gctgataacc ttaagaaaca aggtagctat gttccaggta ttagacctgg
    41641 tgaacaaacc aaaaaatata ttactaaagt tttatatcgt ttaacttttg taggttctat
    41701 cttcttagcc gttatttcaa ttctaccgat attagcaact aagtttatgg gattaccaca
    41761 atcaattcag attggtggta caagtttact tatcgttatt ggtgtagcga ttgaaacaat
    41821 gaaatcttta gaagctcaag tatctcaaaa agaatataaa ggctttggtg gtagataatt
    41881 ttaggagggc acttatgaat atcattttga tgggtttacc tggcgcaggt aaaggaactc
    41941 aagcaagtga aattgtcaag aaattcccaa taccccacat ttcaactggt gacatgttca
    42001 gaaaagctat aaaagaagaa actgaattag gtaaagaagc taagtcttat atggaccgtg
    42061 gcgaattagt tcctgatgaa gtgactgtag gtatcgttaa ggaaagaatt tctgaagacg
    42121 atgcaaaaaa aggcttttta ttagatggct tcccaagaac aatcgagcaa gctgaggcat
    42181 taaataatat tatgtctgag cttgacagaa acattgatgc tgtcatcaat atcgaagttc
    42241 cggaagaaga attaatgaac cgtcttacag gtcgtcgaat ctgtgagtca tgtggtacaa
    42301 cgtatcatct tgtatttaat cctccgaagg tcgaaggtat ttgtgatatc gatggtggta
    42361 aattgtatca acgagaagat gataatcctg aaacggtagc taatcgtttg agtgttaata
    42421 ttaaacaatc taagcctatt ttagatttct atgatcaaaa aggtgtattg aaaaatattg
    42481 atggttcaaa agatattagc gacgttacca aagatgtcat tgatatttta gatcatttgt
    42541 aatagatcaa cttttagcgg tctattttaa gtgaggcatg atttaatcat attagataga
    42601 tgagagtggc aattaacgat aattaattcc cgtaattttt gtttaactta taaaattaat
    42661 cactgataga ttcgtaacaa taaagttgtc tattgacgat taccttacta ttgcaaaaag
    42721 ggggaagtta atcaatggct aaacaagatg taattgaatt agaaggtact gtattagata
    42781 ctttaccgaa cgcaatgttt aaagtagaat tagaaaatgg tcatgagatt ttagctcacg
    42841 taagtggtaa aatcagaatg aattacattc gtattctacc tggcgacaaa gtaactgttg
    42901 agatgtctcc gtacgattta acacgcggaa gaattactta tcgttataaa taatcgtcac
    42961 tccataatat agggaggtat aaaaatgaaa gtaagaccat cagtaaaacc tatttgcgaa
    43021 aaatgtaaag tcattaaacg taaaggtaaa gtaatggtaa tttgtgaaaa tccaaaacac
    43081 aaacaaagac aaggttaata aaagagaggt gtaaattaat atggcacgta ttgcaggagt
    43141 agatattcca cgtgaaaaac gcgtagttat ctcattaact tatatatacg gtatcggtac
    43201 gtcaactgct caaaaaattc ttgaagaagc taacgtatca gctgatactc gtgtgaaaga
    43261 tttaactgat gacgaattag gtcgcatccg tgaagttgta gacggttata aagtcgaagg
    43321 tgacttacgt cgtgaaacta acttaaatat caaacgttta atggaaattt catcataccg
    43381 tggtatccgt caccgtcgtg gtttaccagt tcgtggtcaa aaaacgaaaa acaacgcgcg
    43441 tactcgtaaa ggaccagtta aaacggtagc taacaagaaa aaataatagg taaaggaggc
    43501 aaattttaaa tggcacgtaa acaagtatct cgtaaacgta gagtgaaaaa gaatattgaa
    43561 aatggtgtag cacacatccg ttcaacattc aacaacacta ttgtaactat cactgatgag
    43621 ttcggtaatg ctttatcatg gtcatcagct ggtgcattag gattcaaagg atctaaaaaa
    43681 tcaacaccat ttgcagcaca aatggcttct gaaactgcat ctaaatcagc tatggagcat
    43741 ggtttaaaaa cagttgaagt aacagttaaa ggacctggtc caggtcgtga atcagctatt
    43801 cgtgcattac aatctgcagg tttagaagta actgcgatca gagacgttac tccagtacct
    43861 cataacggtt gtcgtccacc aaaacgtcgt cgtgtataat ttatgatggt attgttacag
    43921 gtcactgagc aaacatttta aattaagtcg acgtatataa ggaggatatt taaatgatag
    43981 aaatcgaaaa acctagaatt gagacaattg aaattagtga agatgctaaa ttcggtaagt
    44041 tcgttgttga accactagaa cgtggctacg gtactacact aggaaactcc ttacgtcgta
    44101 tcctactatc ttcattacca ggtgcagccg ttaagtatat tgaaattgag ggagttttac
    44161 atgaattctc agcagtagac aatgtagttg aagatgtttc tacaattatt atgaacatta
    44221 aacaattagc attgaaaatt tactctgaag aagataaaac tttagaaatt gatgtacgtg
    44281 atgaaggcga agtaacagca agcgacatta cacatgatag tgatgttgaa attttaaacc
    44341 cagagcttaa aattgcaaca gtatctaaag gtggtcactt aaaaattcgt ctagttgcta
    44401 acaagggtag aggttacgca ttagcagaac aaaataatac tagtgattta ccaattggtg
    44461 taatccctgt tgattcattg tattcacctg ttgaacgtgt gaactatact gttgaaaata
    44521 cacgtgtagg tcaaagcagt gattttgata aattaacatt ggatgtttgg actaatggtt
    44581 caatcacacc acaagaatca gtttcattag cagcaaaaat aatgactgaa cacttgaata
    44641 tcttcgttgg tcttactgat gaagcgcaaa acgctgaaat catgattgaa aaagaagaag
    44701 atcaaaaaga aaaagtatta gaaatgtcta ttgaagaatt agacttatct gtacgttcat
    44761 ataactgctt aaaacgcgca ggaatcaatt ctgttcaaga gttagctgac aaatctgaag
    44821 ctgacatgat gaaagtgcgt aatttaggtc gtaaatcttt agaagaagtt aaatacaaat
    44881 tagaagattt aggattagga ttaagaaaag aagattgata aaggaggtta actcatgggt
    44941 tacagaaaat taggtcgtac ttctgatcaa cgtaaagcta tgttacgtga cttagctaca
    45001 tcacttatta ttagtgaacg tattgaaact acagaagctc gtgcaaaaga agttcgcagt
    45061 gttgttgaga aattaatcac tttaggtaaa aaaggagatt tagcttctcg tcgtaatgca
    45121 gctaaaactt tacgtaatgt tgaaatctta aacgaagatg aaactacaca aactgcactt
    45181 caaaaattat ttggtgaaat cgcagagcgt tacacagaac gtcaaggtgg ttacactcgt
    45241 atccttaaac aaggccctcg tcgcggtgac ggtgctgaat cagtaattat cgaattagta
    45301 taagaattta tataaataca ctaactactt ttatataaag caaatgagtg caacgataat
    45361 gtttgccaca tacaaatatt gtctagctca gagtacccca tcaactaaat aaagatttaa
    45421 agcgtgaact caacaaactt atgatggggt gcgcgctttt ttattgaata aaatatcgga
    45481 cttatgaaca gaaatgtttt taagtccgat ttctttatat ttactttttt gagtgcgaat
    45541 ctaaaaatat gatgaagcta gtttgatcaa tttcactaga actgaatgaa gtgaaagtat
    45601 tatcgttgaa tcaaacgcga ccacccctat attagtaaat catttatttt attaacgttt
    45661 atacataatt gttagagagg aataaaagtc cttatcatta gttgttaaac aacactttaa
    45721 agactagaaa tgagtcagaa aatactagta atatatagag aacccagtca atgtcatata
    45781 cagcgattta taaatattgt aaaatatgat agatacacga atcgtaaagg agagagacaa
    45841 gtggaggata agaattcagt tattgtattt aaaaatgttt catttcaata tcaaagtgat
    45901 gcatccttca cattgaaaga tgtttctttt aatataccta aaggtcagtg gacatctatt
    45961 gttggtcata acggttctgg aaaatctaca attgccaagt taatgattgg catagagaaa
    46021 gttaaatctg gagaaatttt ttataataat caagctataa ctgatgataa ttttgaaaag
    46081 ttaagaaaag acataggaat tgtatttcag aatccggata atcaatttgt tggttcaatt
    46141 gtaaaatacg atgtggcatt tggactcgaa aatcatgcgg ttccatatga cgaaatgcat
    46201 agaagagtca gcgaagcact taaacaagtt gatatgttag aacgtgcaga ttatgaacct
    46261 aatgcattat cggggggaca gaagcagcgt gtggctatag caagtgtatt agcacttaac
    46321 ccctctgtca ttatattaga tgaggcgact tctatgttag atcctgatgc acgtcaaaat
    46381 ttattggatt tagtgagaaa agttaaatca gaacataata ttacaatcat ttctattacg
    46441 catgatttat ctgaggcgat ggaagcagat catgttatcg ttatgaataa agggactgtc
    46501 tataaagaag gcacagcgac tgaaattttc gacaatgcag aagagttaac aagaataggt
    46561 ctagatttgc cattcccaat caaaataaat caaatgctgg gacaccaaac atcattctta
    46621 acttatgaag ggctggtgga tcaactatga ctatacggtt tgacaatgta agttatacct
    46681 atcaaaaagg gacaccatat cagcatcaag ctattcatga tgttaataca gaatttgaac
    46741 aaggtaaata ttacgccatc gttggacaaa cgggtagtgg taaatcaacg ttgatacaaa
    46801 atattaatgc gctgttaaag ccgactactg ggacagttac agttgatgac attactatta
    46861 cacataagac caaagataaa tatattagac ctgtaagaaa aagaattgga atggtatttc
    46921 aatttcccga atctcaatta tttgaggaca cagtagagcg tgaaatgata tttggaccta
    46981 aaaactttaa aatgaattta gatgaagcca aaaactatgc ccatcgtctg ttgatggatt
    47041 taggcttttc aagagatgta atgtcacaat caccatttca aatgtcaggt ggacaaatgc
    47101 gtaaaatagc gattgtatcg atattggcaa tgaatcctga tattatcgtg gttgatgaac
    47161 ctacagcagg acttgatcca caaagtaaac gacaagtaat gagattacta aagtcactac
    47221 aaacagatga aaataaggca attatcctaa tttcacatga tatgaatgaa gtcgcgcgtt
    47281 atgcagatga agtcatcgtt atgaaagaag gtagtatagt gtcgcaaaca tcaccaaaag
    47341 agctcttcaa agataaaaaa aaattggcgg attggcatat tggtttgcca gaaattgttc
    47401 agttacaata tgactttgaa caaaaatatc aaacaaaatt aaaagatatt gccttaactg
    47461 aagaagcatt tgtaagcttg tataaggagt ggcaacatga aaaataaatt gattataggg
    47521 cgatatttac caattaattc ttttgttcat catcttgatc caagagcaaa gttaatgttt
    47581 gtttttcttt ttattatatt aatatttttc tgtcattcac cattaacata tctttgggtg
    47641 tttgcactta tcttattcat tatgagatta gcaaaaattc aattatggtt cctaatcaaa
    47701 ggtttaacac caatattttt ctttttaatc ttcacattaa tgatgcatat atttttaact
    47761 aaaggtggat atgtattagt tgaatggcat ggcatcacga ttgaaactaa tggtatttta
    47821 gaagggctat atatatcact gcgtttaatt gggattgtaa tgattgcaac aattatgaca
    47881 ctatctacaa gtccaattga tttaacagat gcgtttgaaa gattacttgc accactaaag
    47941 atgtttaaat taccagttca tcaattaagt atgataatgt ccattgcgtt acgattcatc
    48001 ccgacgttaa tggatgagtt agataaaatc attttggcgc aaaagtcgcg tggttctgaa
    48061 ataagttcag ggaacattgc aacacgtatt aaatcattta ttccattact agtgccacta
    48121 ttcatctcag ctttccaacg cgccgaagaa ttagcggtcg caatggaagt taggggttat
    48181 gatgccaatg ttaagagaac gagctacaga cagcttaaat ggcaattgag agatacgata
    48241 tctttaacca tgattatccc aattgcaatt attttattcg tattaaaata ttcaggagtg
    48301 taaattcatg cgtatattag tagaaattgc gtatcaagga aataattttc taggttttca
    48361 aattcaacaa aatggtcgaa cggtacagca acaatttgaa aagctattac aacgcatgca
    48421 taaaagacat gtgagaatcc atccttcaag taggactgat agaggtgtac atgcgataca
    48481 gcaatacttt catttcgaca cagagctgaa tatacctatg tcacaatggc aatatgcaat
    48541 gaaccgaacg ttacctgatg atatttatgt caacaatgta gttacagtcg atgacgattt
    48601 tcattgtcgt tatgattgtg taggtaaacg ttatcgctac aaagtatatc aagcacaaca
    48661 ccgcgatcca tttcaaagtg gtttgaaaac atttattcct gaaacgttgg atttaggtaa
    48721 aatgaacaga gcggcgcaac aattcatagg tacacatgat tttacgggat tttgttcgca
    48781 aaaaactgaa gtagaaagca aagttagaac attataccaa agtgaaatcg taaagacaga
    48841 tgatggcttt gattatattg tcactggatc tggattttta tataacatgg tgcgtgtctt
    48901 agttgcattt ttaatagaag ttggaaaagg gcgacatgaa gtatctgatg taccaaaact
    48961 tcttgaatca aagaatcgta agaatgtacc tttcactgca ccggctgaag gattatattt
    49021 agagaaaatt tatttagatg aaaatgagtt attaaaagac tttggtaacg atatcaaaat
    49081 acatcgtaaa aaatcattac aaaatgactg attgccattg acaaaaagct tgtgaaatta
    49141 taagattatt aacggtattg ttttatatcc accccacgat aagccccgga aacttattgt
    49201 gttacaagat atataagcag aaacgaacaa cagttaacaa aataaatgaa attaaacgtt
    49261 ttaaaaatga aacaaatgaa atcatctatt aggttatgaa actgtttata gcttgaatag
    49321 aagcatttat tttttaggag gacaattatt atgcgtcaaa catttatggc aaatgaatca
    49381 aacattgagc gcaaatggta tgttatcgat gctgaaggcc aaacattagg tcgtttatca
    49441 tcagaagtag catctatctt acgcggtaaa aataaagtaa cttacacacc acacgttgat
    49501 actggtgatt atgtaatcgt tattaatgca tcaaaaatcg aatttactgg taacaaagaa
    49561 actgacaaag tttactaccg tcactcaaat cacccaggtg gtatcaaatc aatcactgct
    49621 ggtgaattaa gaagaactaa cccagaacgt ttaattgaaa actcaattaa aggtatgtta
    49681 ccaagcactc gtttaggcga aaaacaaggt aaaaaattat ttgtatatgg tggcgctgaa
    49741 catccacacg ctgcacaaca accagaaaac tacgaattac gtggttaatt agaaggagga
    49801 aatgactttg gcacaagttg aatatagagg cacaggccgt cgtaaaaact cagtagcacg
    49861 tgtacgttta gtaccaggtg aaggtaacat cacagttaat aaccgtgacg tacgcgaata
    49921 cttaccattc gaatcattaa ttttagactt aaaccaacca tttgatgtaa ctgaaactaa
    49981 aggtaactat gatgttttag ttaacgttca tggtggtggt ttcactggac aagctcaagc
    50041 tatccgtcac ggaatcgctc gtgcattatt agaagcagat cctgaataca gaggttcttt
    50101 aaaacgcgct ggattactta ctcgtgaccc acgtatgaaa gaacgtaaaa aaccaggtct
    50161 taaagcagct cgtcgttcac ctcaattctc aaaacgttaa ttgtcggacg atatatacaa
    50221 aacacctcga tattatgtcg aggtgttttt ttgcgttttt gcggcgaata tggaatgtgt
    50281 agaatataaa tgaattttta ccttcccacc ataaaagatg aagaaccatg aaatgtggag
    50341 aacaataaat agttgatatt ctgttatttt tttggaagtg aagtgatttg aatactttac
    50401 tctaaacgat aaaaggttaa aaaaacaaca aaaagaaaat attactgtca taattggaaa
    50461 tacaccgtat tctgctggtc aatctagtgt aaataatgat aataaaaaaa tgaatatgaa
    50521 aaattaaatg caaaaatagc aaatacctat gcaaattttt caacagcaaa tttaaaaaaa
    50581 tcattatatg attcgtatat acaggctttt cgatgggcaa ctgatagagt tggaaaaaag
    50641 gtattatagg atttgtaaca aatggatcat ttattgatag tcaaagcact gatggattta
    50701 gaaaagtttt atacgatgaa tttaactatc tgtacataat taatttgaga ggcgaccaac
    50761 gaacacaggg tgaaaaatca agaaaagaag gtggaaaaat atttggatca ggaagtagag
    50821 caccgatagc tatatctatt ttagttaaag atggaagtta caatcacgat atctattata
    50881 atgacattgg tgaatattta actagagagc aaaaattaga tactttaatg aaacatcaat
    50941 caattgtgaa tttgaaaagt ttgaatgttt tacccgataa aaacaatgat tggattaatc
    51001 aacgagacat aaactatgag aattatttac caatgtatga ttccaaggat attgaaaatt
    51061 ctatatattt agatcaattt aatggagtaa attcagcaag agataattgg gtaacaaatt
    51121 tttcgaatga aaaagcatta gtaaatgcaa aattacttgt agataactat aattctgaaa
    51181 tagatagatt aattgatatc ttagattcaa gagaacgtat aaatttagtg aacaaagatg
    51241 aaacatttat tagttggaca cgaggactta cccaaaaatt ttctaaagga aaaaatattt
    51301 caattaatcc agagagaatt gttaaattta tgcatagacc atttacaaaa aaatggatag
    51361 tgtacgacaa aaatattatg gaaatgccaa gtagatatta taatataatg gagaatactg
    51421 gacaggtgat ttatatacaa ggacagggaa tgaataaaga attctcggct atgatcacgg
    51481 acatattacc taatttccaa tttattggta atggcaaagg ctttgctaca tacaaaggta
    51541 aagatagttt gaggcttgta g
//
LOCUS       DAKTLH010000005        48266 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.005, whole genome shotgun sequence.
ACCESSION   DAKTLH010000005 DAKTLH010000000
VERSION     DAKTLH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 48266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48266
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.005"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            135..1394
                     /locus_tag="O0394_000212"
     CDS             135..1394
                     /locus_tag="O0394_000212"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCY0498328.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            1483..1818
                     /locus_tag="O0394_000213"
     CDS             1483..1818
                     /locus_tag="O0394_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0498329.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            2056..3483
                     /locus_tag="O0394_000214"
     CDS             2056..3483
                     /locus_tag="O0394_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCY0498330.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
     gene            3730..5046
                     /gene="rho"
                     /locus_tag="O0394_000215"
     CDS             3730..5046
                     /gene="rho"
                     /locus_tag="O0394_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCY0498331.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            5164..5418
                     /locus_tag="O0394_000216"
     CDS             5164..5418
                     /locus_tag="O0394_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCY0498332.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            5765..6364
                     /locus_tag="O0394_000217"
     CDS             5765..6364
                     /locus_tag="O0394_000217"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCY0498333.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            6365..7441
                     /gene="prfA"
                     /locus_tag="O0394_000218"
     CDS             6365..7441
                     /gene="prfA"
                     /locus_tag="O0394_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCY0498334.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            7428..8264
                     /gene="prmC"
                     /locus_tag="O0394_000219"
     CDS             7428..8264
                     /gene="prmC"
                     /locus_tag="O0394_000219"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCY0498335.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            8348..9394
                     /locus_tag="O0394_000220"
     CDS             8348..9394
                     /locus_tag="O0394_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCY0498336.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKLGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            9391..9810
                     /locus_tag="O0394_000221"
     CDS             9391..9810
                     /locus_tag="O0394_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HCY0498337.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            9917..10441
                     /locus_tag="O0394_000222"
     CDS             9917..10441
                     /locus_tag="O0394_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCY0498338.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            10468..11706
                     /locus_tag="O0394_000223"
     CDS             10468..11706
                     /locus_tag="O0394_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCY0498339.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALGMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            11734..12363
                     /gene="upp"
                     /locus_tag="O0394_000224"
     CDS             11734..12363
                     /gene="upp"
                     /locus_tag="O0394_000224"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCY0498340.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            12387..13514
                     /gene="wecB"
                     /locus_tag="O0394_000225"
     CDS             12387..13514
                     /gene="wecB"
                     /locus_tag="O0394_000225"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCY0498341.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            13582..14034
                     /locus_tag="O0394_000226"
     CDS             13582..14034
                     /locus_tag="O0394_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HCY0498342.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYFYLIIILGGLYLYTHHAFILGLIIGVTGSAINTCIFECYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYLNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            14055..14783
                     /gene="atpB"
                     /locus_tag="O0394_000227"
     CDS             14055..14783
                     /gene="atpB"
                     /locus_tag="O0394_000227"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCY0498343.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            14826..15038
                     /gene="atpE"
                     /locus_tag="O0394_000228"
     CDS             14826..15038
                     /gene="atpE"
                     /locus_tag="O0394_000228"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCY0498344.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            15236..15757
                     /locus_tag="O0394_000229"
     CDS             15236..15757
                     /locus_tag="O0394_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCY0498345.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            15757..16296
                     /locus_tag="O0394_000230"
     CDS             15757..16296
                     /locus_tag="O0394_000230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCY0498346.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLR
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            16318..17826
                     /locus_tag="O0394_000231"
     CDS             16318..17826
                     /locus_tag="O0394_000231"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCY0498347.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            17857..18723
                     /locus_tag="O0394_000232"
     CDS             17857..18723
                     /locus_tag="O0394_000232"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCY0498348.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            18745..20157
                     /gene="atpD"
                     /locus_tag="O0394_000233"
     CDS             18745..20157
                     /gene="atpD"
                     /locus_tag="O0394_000233"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCY0498349.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            20177..20581
                     /locus_tag="O0394_000234"
     CDS             20177..20581
                     /locus_tag="O0394_000234"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCY0498350.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            20919..21011
                     /locus_tag="O0394_000235"
     CDS             20919..21011
                     /locus_tag="O0394_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498351.1"
                     /translation="MLKSKFPSPLSTVDISFFEIIYIFSYQDIL"
     gene            21143..21376
                     /locus_tag="O0394_000236"
     CDS             21143..21376
                     /locus_tag="O0394_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCY0498352.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            21487..22752
                     /gene="murA"
                     /locus_tag="O0394_000237"
     CDS             21487..22752
                     /gene="murA"
                     /locus_tag="O0394_000237"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCY0498353.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRDAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            22786..23226
                     /gene="fabZ"
                     /locus_tag="O0394_000238"
     CDS             22786..23226
                     /gene="fabZ"
                     /locus_tag="O0394_000238"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCY0498354.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(23285..23725)
                     /locus_tag="O0394_000239"
     CDS             complement(23285..23725)
                     /locus_tag="O0394_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HCY0498355.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            23919..24266
                     /locus_tag="O0394_000240"
     CDS             23919..24266
                     /locus_tag="O0394_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002466750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0498356.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSI"
     gene            24703..25398
                     /gene="sceD"
                     /locus_tag="O0394_000241"
     CDS             24703..25398
                     /gene="sceD"
                     /locus_tag="O0394_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HCY0498357.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGTNGADMSAQAPATNNV
                     APSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKSSEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     regulatory      25602..25704
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            25784..26473
                     /gene="tenA"
                     /locus_tag="O0394_000242"
     CDS             25784..26473
                     /gene="tenA"
                     /locus_tag="O0394_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HCY0498358.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLSGDIGADALRHYLKAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     gene            26466..27296
                     /gene="thiD"
                     /locus_tag="O0394_000243"
     CDS             26466..27296
                     /gene="thiD"
                     /locus_tag="O0394_000243"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCY0498359.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            27280..28071
                     /gene="thiM"
                     /locus_tag="O0394_000244"
     CDS             27280..28071
                     /gene="thiM"
                     /locus_tag="O0394_000244"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCY0498360.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYATYKTAIVIT
                     GKEDVIVQDNKAFVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            28073..28714
                     /locus_tag="O0394_000245"
     CDS             28073..28714
                     /locus_tag="O0394_000245"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCY0498361.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            28800..29672
                     /gene="yidC"
                     /locus_tag="O0394_000246"
     CDS             28800..29672
                     /gene="yidC"
                     /locus_tag="O0394_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCY0498362.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(30485..31132)
                     /locus_tag="O0394_000247"
     CDS             complement(30485..31132)
                     /locus_tag="O0394_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCY0498363.1"
                     /translation="MQQSDVISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNCHD"
     gene            complement(31160..32644)
                     /gene="cls"
                     /locus_tag="O0394_000248"
     CDS             complement(31160..32644)
                     /gene="cls"
                     /locus_tag="O0394_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCY0498364.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            32810..33103
                     /gene="csoR"
                     /locus_tag="O0394_000249"
     CDS             32810..33103
                     /gene="csoR"
                     /locus_tag="O0394_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HCY0498365.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            33115..33324
                     /locus_tag="O0394_000250"
     CDS             33115..33324
                     /locus_tag="O0394_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HCY0498366.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVF"
     gene            33452..33589
                     /locus_tag="O0394_000251"
     CDS             33452..33589
                     /locus_tag="O0394_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498367.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(33853..35055)
                     /locus_tag="O0394_000252"
     CDS             complement(33853..35055)
                     /locus_tag="O0394_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HCY0498368.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            35373..36443
                     /locus_tag="O0394_000253"
     CDS             35373..36443
                     /locus_tag="O0394_000253"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCY0498369.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            36458..37816
                     /locus_tag="O0394_000254"
     CDS             36458..37816
                     /locus_tag="O0394_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCY0498370.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLIYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLINDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
     gene            38333..39853
                     /locus_tag="O0394_000255"
     CDS             38333..39853
                     /locus_tag="O0394_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCY0498371.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
     gene            complement(40222..40917)
                     /locus_tag="O0394_000256"
     CDS             complement(40222..40917)
                     /locus_tag="O0394_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0498372.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(40917..43574)
                     /locus_tag="O0394_000257"
     CDS             complement(40917..43574)
                     /locus_tag="O0394_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HCY0498373.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVNLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SYEQIEFAVIDEGPGISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIYTDFNEGDV"
     gene            43712..43822
                     /gene="kdpF"
                     /locus_tag="O0394_000258"
     CDS             43712..43822
                     /gene="kdpF"
                     /locus_tag="O0394_000258"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCY0498374.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            43846..45522
                     /gene="kdpA"
                     /locus_tag="O0394_000259"
     CDS             43846..45522
                     /gene="kdpA"
                     /locus_tag="O0394_000259"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HCY0498375.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPIGGLGPMVLMMLNVVFGGEGVGLMNLLIFVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            45541..47568
                     /gene="kdpB"
                     /locus_tag="O0394_000260"
     CDS             45541..47568
                     /gene="kdpB"
                     /locus_tag="O0394_000260"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCY0498376.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNLKQLIKNPIMFVVEVGMIL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFTELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
     gene            47588..48148
                     /locus_tag="O0394_000261"
     CDS             47588..48148
                     /locus_tag="O0394_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCY0498377.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RMKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHVSTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
BASE COUNT        16942 a         7189 c         9155 g        14980 t
ORIGIN      
        1 gttattaatc aatcgaaaat agaaagacaa gattttaacg attaaaataa actattttac
       61 aaataaagta aaattaattt ataatgctaa taatgcaaaa aattaaaaag taatggacaa
      121 aggagataaa tgatatggct caagaggtaa taaaaataag aggtggacgc acactaaatg
      181 gggaagttaa tatcagtggc gcgaaaaaca gtgctgtagc tatcattcct gcaacattat
      241 tagctcaagg acatgtgaaa ttagaagggt taccgcaaat ctctgatgtt aaaactttag
      301 tgagtttatt agaagattta aatattaagg catcactaaa tggtacggaa ttagaagttg
      361 atacaactga aatacaaaat gctgcattac caaataataa agttgaatca ttacgtgctt
      421 catactatat gatgggtgcc atgttaggta gatttaaaaa atgtgtgatt ggtttaccgg
      481 gaggatgtcc acttgggcca cgtccaattg atcaacatat taaaggattt aaagctttag
      541 gtgctgaaat tgatgaatca agtacgacat caatgaaaat cgaagctaaa gagttaaaag
      601 gtgcacatat ttttctagat atggttagcg taggtgcaac aattaacatt atgctagctg
      661 cagtttatgc aacaggacaa actgtaattg aaaatgctgc taaagaaccg gaagtagttg
      721 atgttgctaa cttcttaaca agtatgggag ctaatattaa gggagcgggt acatcaacaa
      781 ttaaaatcaa tggcgtcaaa gaattacatg gttctgagta tcaagttata cctgatagaa
      841 ttgaagcagg cacatatatg tgtatcgctg cagcatgtgg agaaaatgtt atattaaata
      901 atattgttcc gaaacatgta gaaacattaa ctgctaaatt tagtgaatta ggtgtgaatg
      961 ttgatgtaag agacgaaaga attcgcatca ataataatgc gccatatcaa tttgttgata
     1021 ttaaaacact agtatatcca ggttttgcta ctgatttgca acagcctatt acaccattat
     1081 tatttatggc aaatggtcct tcatttgtaa ctgatacgat ttatccggag cgttttaaac
     1141 atgttgaaga attaaagcgc atgggtgcaa atattgaagt tgacgaaggc acagcaacga
     1201 taaaaccatc aacattacat ggtgctgaag tttatgctag cgatttaaga gcaggagctt
     1261 gtttaattat tgctggttta attgctgagg gtgtaactac aatttacaat gttaaacata
     1321 tttatagagg ttatacagat attgtagaac acttaaaagc tttaggtgca gatatttgga
     1381 cggaaactgt atagatttat cttgttttgt ggtataataa ttatactgat taaaaaatgg
     1441 tgttcgttta cattctaaat ttattgatga gaggtgaaga tgatggaagt atgtccgtat
     1501 ctcgaagaaa cttttaaaat acttggtaga agttggaatg gattaatcat taattatctc
     1561 tcaagatgta atgactgttc agcacacttt tccgatatga aaagagattt gaaaacaata
     1621 acaccacgtg ctttaagtct taagttgtca gagcttgcac aatgggagtt agttgagaag
     1681 caaatcattt ctacgagtcc agtacaaatt atttatgtgc taactgaaaa aggtaaagcg
     1741 ttagcagagg ctttacatcc aattgaagca tgggcgcaat catatgtcga tttaacagat
     1801 caacgtactg ctaaataatt acatgacgtt taaacatagc attgattata actatttcta
     1861 agtcttcgca ttatttgcga tgatgtggga atagttattt ttatttaaaa atataaaaaa
     1921 atagatgcag caaaatttta aagcatttta ttttgaacat attaaaaggg agcgtatcat
     1981 aatggaatgt aatgtttata tcgtatgcat tacggataaa taatatataa atcattcttg
     2041 aggagtgaaa gaataatgag agactacaca aagcaataca ttaatggcga atgggtagaa
     2101 agtaatagta atgaaacgat agaagttata aatccagcaa ccgaagaagt aatcgggaaa
     2161 gttgctaaag gtaataaagc tgatgttgat aaagccgtcg aggcggcaga cgatgtttat
     2221 ttagagttcc gtcatacatc tgtgaaagaa agacaagcgt tattagataa aattgtaaaa
     2281 gaatatgaaa acagaaaaga cgatattgta caagctatta cggatgaatt aggtgctcct
     2341 ttatcattat ctgagcgtgt ccattatcaa atgggactaa accattttgt tgcagcgaga
     2401 gacgcattag ataactacga atttgaagaa cgccgcggag atgatttagt tgttaaagaa
     2461 gcaatcggtg tatctggatt aattacaccg tggaacttcc ctacaaacca aacatcatta
     2521 aaattagcag cagcatttgc ggctggtagt ccagttgtac ttaaaccatc tgaagaaaca
     2581 ccatttgcag ctgttatttt agctgagatt tttgataaag tcggtgttcc taaaggtgta
     2641 tttaaccttg ttaatggtga tggtgctggt gttgggaatc ctttatctga acatcctaaa
     2701 gtacgcatga tgtcatttac aggatcaggc cctactggtt ctaaaattat ggaaaaagcc
     2761 gctaaagatt ttaaaaaggt atcattagag cttggtggca aatcaccata tatcgtccta
     2821 gatgacgtag atattaaaga agcggctaaa gcaacaacag gcaaagttgt taataatact
     2881 ggtcaagtat gtacagctgg tacacgtgtt ttagtgccta acaaaattaa agatgcattc
     2941 ttagctgaat taaaagaaca atttagccaa gtgcgtgtcg gtaatccaag agaagatggt
     3001 acacaagtag gccctatcat tagtaaaaaa caatttgatc aagtacaaaa ttatattaat
     3061 aaaggtattg aagaaggtgc tgaattattt tatggtggtc ctggtaaacc agaaggactt
     3121 gaaaaaggat actttgcacg tccgacaatt tttattaatg tagataatca aatgacgata
     3181 gcacaagaag aaatttttgg gccagtaatg tcagttatca cttataacga tttagatgaa
     3241 gcgattcaaa ttgcaaatga tacaaaatat ggtttggcag gatatgttat tggtaaggac
     3301 aaagaaacat tgcataaagt agctcgttct attgaagcag gtacagtaga aataaacgaa
     3361 gcaggtagaa agccagattt accatttggt ggctataaac aatctggttt aggtcgtgaa
     3421 tggggcgatt atggtattga agagttctta gaagtgaaat ctatagctgg atattttaaa
     3481 taaatttgat ttttgaatta aaaatcgcaa taaaacagtg cacatgacta attaagtttt
     3541 gtgtactgtt ttaattttgc aatttttata aatagatttt gtaattaaaa taaaaatttg
     3601 ctatagttat tcatgtattt aaaaggttgg ggattagcat aatgggattg tgctagcaca
     3661 gttatttatg cattgtcatg cctatctatt acttactaac taaaaaataa tgaaatgggt
     3721 gtaaactata tgcctgaaag agaacgtaca tctcctcagt atgaatcatt ccacgaattg
     3781 tacaagaact atactaccaa ggaactcact caaaaagcta aaactcttaa gttgacgaac
     3841 tatagtaaat taaataaaaa agaacttgtt ctagctatta tggaagcaca aatggaaaaa
     3901 gatggtaact attatatgga aggtatctta gatgatatac aaccagatgg ttatggtttt
     3961 ttaagaacag tgaactattc taaaggggaa aaagatattt atatatctgc tagccaaatt
     4021 cgtcgttttg aaattaaacg tggggataaa gtaactggga aagttagaaa acctaaagat
     4081 aacgaaaaat attatggctt attacaagtt gactttgtca atgaccataa cgcagaagaa
     4141 gtgaagaaac gtccgcattt ccaagctttg acaccacttt atccagatga gcgtattaaa
     4201 ttagagacag aaacacaaaa ttattcaacg cgcatcatgg atttagtaac accgattggt
     4261 ttaggtcaac gtggtttaat agtggcgcca cctaaagcag gtaaaacatc gttattaaaa
     4321 gaaatagcga atgcaatcag tacgaacaaa ccagatgcaa agctatttat tttgttagtt
     4381 ggcgagcgtc ctgaagaggt aacagattta gaacgctcag tagaagctgc tgaagtcgtt
     4441 cattcaacgt ttgacgaacc accagaacac catgttaaag tagctgaatt attacttgaa
     4501 cgtgcaaagc gtttagtaga aattggggaa gatgtcatta ttttaatgga ttctataacg
     4561 agattagcac gcgcttataa cttagttatt ccaccaagtg gtcgtacatt atcaggtggt
     4621 ttagatcctg catctttaca caaaccaaaa gcattcttcg gtgcagcgag aaatattgaa
     4681 gcgggtggaa gtttaacaat acttgcaact gcattagttg atacgggttc acgtatggac
     4741 gatatgattt acgaagaatt taaaggaaca ggtaacatgg agttacattt agatcgtaaa
     4801 ttgtctgaac gtcgtatctt ccctgcaatt gatattggca gaagttcaac gcgtaaagaa
     4861 gaattgttga taagtaaatc tgaattagac acattatggc aattaagaaa tctattcact
     4921 gactcaactg actttactga aagatttatt cgcaaactta aaaggtctaa gaataatgaa
     4981 gatttcttca agcagctaca aaagtctgca gaagaaagta ctaaaacggg tcgacctata
     5041 atttaataaa cattatatag gggcttgcgt tttgaattaa ttacctttat aattacacag
     5101 tattgggtaa aaactcacaa ataactctgt tccagatggt tcagggcaaa ggagctgaaa
     5161 ataatgaaac aaggaattca tcctgagtat caccaagtaa tctttttaga tactactaca
     5221 aacttcaaat tcttaagcgg ttctacaaaa acatcttcag aaatgatgga gtgggaagat
     5281 ggaaaagaat acccagttat tcgtttagat atttcatctg attcacatcc attctacact
     5341 ggacgtcaaa aattcgctgc tgcggatgga cgtgtggaac gtttcaacaa aaagtttggt
     5401 cttaaatcaa acaactaata tagtaatcgt taagctgtct gcatcctata atggaattgt
     5461 agacagcttt tttatttata agcgtagtaa gggtatatac ttacgactgt gttcaaagaa
     5521 gtgactcaat accatgttgt taatacataa tgataaattt ccccctttat taaagtttgt
     5581 attgagtaga aacatcgcac gaataagaag caacaaatta gaatagctgt atataaatat
     5641 gcaagacaaa tttataaaat aattgttcat gtacaatatc gatataatta tgaaaaagac
     5701 atttatgaat atttgaaata tgatataata aagcgattat gttttgaaaa aggtggaact
     5761 ctcaatgtat gaaacttacc attccggttg gatagaatgt attacaggta gtatgtttag
     5821 cggtaaatcc gaagaactaa taagacgtct aagaaggggt atatacgcta aacaaaaagt
     5881 cgttgtattt aaaccggcga ttgatgatcg atatcataaa gaaaaagtgg tctctcataa
     5941 tggtaatgcc attgaagcga ttaatatttc taaagcaagt gaaattatga cgcacgactt
     6001 aactaatgtt gatgttatag gaatagacga agtgcaattt tttgacgatg aaattgtaag
     6061 tatagtagaa aaactatcag cagatggaca ccgtgttatt gttgctggat tagatatgga
     6121 ttttaggggc gaaccgttcg aaccaatgcc taaattgatg gctgtcagtg aacaagtaac
     6181 gaaacttcaa gcagtttgtg ctgtttgtgg ttcttcatct agtagaacgc aacgattaat
     6241 aaatggtaaa ccagctaaaa tcgatgatcc gattatctta gtaggtgcca atgaaagtta
     6301 tgagccacgt tgtcgcgcgc accatatcgt agcacctagt gataataata aggaggaatt
     6361 ataagtgttt gatcaattag atattgtaga agaaagatac gaacagttaa atgaactgtt
     6421 aagtgaccca gatgttgtaa atgattcaga taaattacgt aaatattcta aagagcaagc
     6481 tgatttacaa aaaactgtag atgtttatcg taactataaa gctaaaaaag aagaattagc
     6541 tgatattgaa gaaatgttaa gtgagactga tgataaagaa gaagtagaaa tgttaaaaga
     6601 ggagagtaat ggtattaaag ctgaacttcc aaatcttgaa gaagagctta aaatattatt
     6661 gattcctaaa gatcctaatg atgacaaaga cgttattgta gaaataagag cagcagcagg
     6721 tggtgatgag gctgcgattt ttgctggtga tttaatgcgt atgtattcaa agtatgctga
     6781 atcacaagga ttcaaaactg aaatagtaga agcgtctgaa agtgaccatg gtggttacaa
     6841 agaaattagt ttctcagttt ctggtaatgg cgcgtatagt aaattgaaat ttgaaaatgg
     6901 tgcgcaccgc gttcaacgtg tgcctgaaac agaatcaggt ggacgtattc atacttcaac
     6961 agctacagtg gcagttttac cagaagttga agatgtagaa attgaaatta gaaatgaaga
     7021 tttaaaaatc gacacgtatc gttcaagtgg tgcaggtggt cagcacgtaa acacaactga
     7081 ctctgcagta cgtattaccc atttaccaac tggtgtcatt gcaacatctt ctgagaagtc
     7141 tcaaattcaa aaccgtgaaa aagcaatgaa agtgttaaaa gcacgtttat acgatatgaa
     7201 agttcaagaa gaacaacaaa agtatgcgtc acaacgtaaa tcagcagtcg gtactggtga
     7261 tcgttcagaa cgtattcgaa cttataatta tccacaaagc cgtgtaacag accatcgtat
     7321 aggtctaacg cttcaaaaat tagggcaaat tatggaaggc catttagaag aaattataga
     7381 tgcactgact ttatcagagc agacagataa attgaaagaa cttaataatg gtgaattata
     7441 aagaaaagtt agatgaagca attcatttaa cacaacaaaa agggtttgaa caaacacgag
     7501 ctgaatggtt aatgttagat gtatttcaat ggacgcgtac ggactttgta gtccacatgc
     7561 atgatgatat gccgaaagcg atgattatga agttcgactt agcattacaa cgtatgttat
     7621 taggagagcc tatacagtat atagttggct ttgcctcatt ttatggtaga acgtttgatg
     7681 taaactcaaa ttgtttgata ccaagacctg aaactgaaga agtaatgttg catttcttac
     7741 aacagttaga agatgatgca acaatcgtag atatcggaac gggtagtggt gtacttgcaa
     7801 ttactttgaa atgtgaaaag ccggatttaa atgttattgc tactgatatt tcacttgaag
     7861 caatgaatat ggctcgtaat aatgctgaga agcatcaatc acaaatacaa tttttaacag
     7921 gggatgcatt aaagccctta attaatgaag gtatcaagtt gaacggcttg atatctaatc
     7981 caccatatat agatgaaaaa gatatggtta cgatgtctcc aacggttacg agattcgaac
     8041 cacatcaggc attgtttgca gataaccatg gatatgctat ttatgaatca atcattgaag
     8101 atttacctca cgttatggaa aaaggcagcc cagttgtttt tgaaattggt tacaatcaag
     8161 gtgaggcact taaatcaata attttaaata aatttcctga caaaaaaatc gacattatta
     8221 aagatataaa tggccacgat cgaatcgtct catttaaatg gtaattagaa gttatgcctt
     8281 tgctatgatt agttaagtgc atagcttttt gctttatatt atgataaata agaaaggcgt
     8341 gattaagttg gatactaaaa tttgggatgt tagagaatat aatgaagatt tacagcaata
     8401 tcctaaaatt aatgaaataa aagacattgt tttaaacggt ggtttaatag gtttaccaac
     8461 tgaaacagtt tatggacttg cagcaaatgc gacagatgaa gaagctgtag ctaaaatata
     8521 tgaagctaaa ggccgtccat ctgacaatcc gcttattgtt catatacaca gtaaaggtca
     8581 attaaaagat tttacatata ctttggatcc acgcgtagaa aagttaatgc aggcattctg
     8641 gccgggccct atttcgttta tattgccgtt aaagctaggc tatctatgtc gaaaagtttc
     8701 tggaggttta tcatcagttg ctgttagaat gccaagccat tctgtaggta gacaattatt
     8761 acaaatcata aatgaacctc tagctgctcc aagtgctaat ttaagtggta gaccttcacc
     8821 aacaactttc aatcatgtat atcaagattt gaatggccgt atcgatggta ttgttcaagc
     8881 tgaacaaagt gaagaaggat tagaaagtac ggttttagat tgcacatctt ttccttataa
     8941 aattgcaaga cctggttcta taacagcagc aatgattaca gaaatacttc cgaatagtat
     9001 cgcccatgct gattataatg atactgaaca gccaattgca ccaggtatga agtataagca
     9061 ttactcaccc aatacaccac ttacaattat tacagatatt gagagcaaaa ttggaaatga
     9121 cggtaaagat tggtcttcta tagcttttat tgtgccgagt aataaggtgg cgtttatacc
     9181 aagtgaagcg caattcattc aattatgtca ggatgacaat gatgttaaac aagcaagtca
     9241 taatctttat gatgtgttac attcacttga tgaaaatgaa aatatttcag cggcgtatat
     9301 atacggcttt gagctgaatg ataatacaga agcaattatg aatcgcatgt taaaagctgc
     9361 aggtaatcac attattaaag gatgtgaact atgaagattt tattcgtttg tacaggtaac
     9421 acatgtcgta gcccattagc ggaaagtatt gcaaaagagg ttatgccaaa tcatcaattt
     9481 gaatcaagag gtatattcgc tgtgaacaat caaggtgttt cgaattatgt tgaagactta
     9541 gttgaagaac atcatttagc tgaaacgacc ttatcgcaac aatttactga agcagatttg
     9601 aaagcagata ttattttgac gatgtcgtat tcgcacaaag aattaataga ggcacacttt
     9661 ggtttgcaaa atcatgtttt cacattgcat gaatatgtaa aagaagcagg agaagttata
     9721 gatccatacg gtggaacaaa agaaatgtat gtacatacct atgaagaact tgtaagttta
     9781 attttaaaat taaaagatat tatttgctag taggaattct gtataattag tatatgttaa
     9841 cagttaaata agttataata gaggagtgta gcactttgct atattccttt tttattttta
     9901 ggaagggtgg gcttttatga aagatttgac aatgttatta gacgaattaa aagatatgtc
     9961 cttttttaat aaaggagata tatgtttaat tggatgttca acatcggaag tcattggcga
    10021 aaaaataggg actgtcggtt cgatggaagt tgcagaaact atttttaatg cattagatgt
    10081 tgtgagtaaa gagacaggcg ttacttttgc ttttcaagga tgtgaacata tcaacagagc
    10141 gataacaata gaaaaatctc agtataatcc attaacaatg gaagaagttt ccgtcgttcc
    10201 agatgtacat gctggaggga gtttggcaac atacgctttt cagcatatga aggatccgat
    10261 cgtggtcgaa catattactg ttccttgtgg aattgatatt ggtcaaacgt taataggcat
    10321 gcatattaag catgtatgtg tacctgtaag gacctcagta aagcaagtag gacaggcaat
    10381 tgtaactatt gccacatcta ggccgaaaaa gattggtggc gaacgtgcta aataccaata
    10441 agtttttttg aaagaagggg agattttatg tcttatatca ccaagcaaga taaagttatc
    10501 gcagaagcaa tcgagagaga atttcagaga caaaatagca acatagagtt aatcgcatcg
    10561 gaaaattttg tatcggaagc ggttatggaa gcacaaggtt cagtgttgac taataagtat
    10621 gctgaaggct atccaggacg ccgatattat ggtggctgtg agtttgtaga tgttactgaa
    10681 agcatcgcaa ttgatcgtgc taaagcattg tttggagctg aacatgtcaa tgttcaacca
    10741 cattcaggtt cacaagcgaa catggctgtt tacttagttg cattaggaat gggcgacaca
    10801 gttttaggta tgaatttgag tcatggtggt cacttgacac atggagcgcc tgttaatttt
    10861 agtggtaaat tctacaattt cgttgaatat ggagtagata aagacacaga acgaatcaat
    10921 tatgatgaag ttcgtaaatt agcgttagag cataagccta agcttattgt ggcaggagca
    10981 tcagcatatt caagaacaat tgacttcaaa aagtttaaag aaatcgcaga tgaagtaaac
    11041 gctaagttaa tggtagacat ggcacatatt gcaggattag tagcggcagg tttacatcca
    11101 aatccagtag aatatgctga ttttgtaaca actacaacac acaaaacatt acgcggacca
    11161 cgtggtggta tgattttatg taaggaagaa tataaaaaag acatagataa aacaattttc
    11221 cctggtattc aaggtggacc tcttgagcat gttattgcag caaaagcagt tgcttttgga
    11281 gaagcgttag aaaataattt caaaacgtat caacaacaag tggttaaaaa cgcaaaagtt
    11341 cttgcagaag cattaattaa tgaaggattt agaattgttt ctggcggtac agataatcac
    11401 ttagtagctg ttgatgtaaa agggtctata ggacttactg gtaaagaagc tgaagagact
    11461 ttagattcag ttggtatcac atgtaacaaa aataccattc cgttcgatca agaaaaacct
    11521 tttgtaacga gtggtatacg tttaggtaca cctgctgcaa caacgcgtgg atttgatgaa
    11581 aaagcttttg aggaagttgc aaaaatcatc agtttagcat tgaaaaatag taaagatgaa
    11641 gaaaaattac aacaagctaa agaacgcgtt gcgaaattaa cagctgaata tcctctatat
    11701 caataaacat acacaaagat aggagtaatt tttatgagta aagtacacgt tttcgatcat
    11761 ccattaattc aacacaaact aagctatatt agagatgtaa atacaggtac taaagaattt
    11821 agagaacttg tagacgaagt tggtatgttg atggcatatg aagtaactag agacttagag
    11881 cttcaggatg ttgatattga aacgcctgta actaaaatga cggctaaacg attagctggt
    11941 aaaaaattag ctatcgttcc aattttaaga gctggtttag gtatgactga tggtatttta
    12001 agtttagtac ctgcagccag agttggacat atcggtttat atcgagatcc cgaaacatta
    12061 aaggctgtag agtattttgc taagttacct caagatatta ctgaaagaca aatcattgtt
    12121 gttgatccta tgttagcaac aggcgcatca gcaattgaag ctattacttc attgaagaaa
    12181 cgtggcgcta aaaatattcg cttcatgtgt ttaattgcag caccagaagg cgtagaaaag
    12241 atgcatgaag cacatccaga tgtagatatt tatattgctg cacttgatga aaagctaaat
    12301 gacaaagcat atatcacacc agggttaggc gacgctggag atagattgtt tggtactaaa
    12361 tagaagaatc ggagataagt agtttgatga aaaagattat gaccatattt ggaacaagac
    12421 ccgaagccat aaagatggct cctttagtta aagctttgga acaagaaaaa atgctcgagc
    12481 caattgttgt tgttacagca cagcatagag aaatgcttga ttcagtatta agtacctttg
    12541 aaatcaaacc aaaatacgat ttgaatatta tgaaatcagg acagacactt tctgagataa
    12601 cttctaaatc aatcactcaa ttagaacaag tgattcaatt agaaaaacca gacatggtac
    12661 ttgtacatgg tgatacgatg acgacatttg caggtggttt agcggcattt tataaccaag
    12721 tgccgattgg acacgttgaa gcagggctta gaagttatga taaatattca ccttttccag
    12781 aagaggtgaa tagacaatta gttggtgtat tagctgactt acattttgca cctactaaaa
    12841 atgctgcatc gcatttgtta agtgaaggga aatattcaga aagtgttgtt gtcactggta
    12901 atactgcgat tgatgctatg aaatatacag ttgatgacaa ttataaatct aacatcatgg
    12961 ataaatacca tgacaaaaaa ttcattctta tgacagcaca ccgacgagaa aatatcggga
    13021 aacccatgga aaatatattt aaagcggtta gacgcttaat agatgagtat actgatttag
    13081 cactggtata tccaatgcat aaaaacccga aagttcgaga ggtggctcaa aagattttag
    13141 gtagtcatga cagaattgaa ttgattgaac ctttggacgt agtagatttc cataattttg
    13201 ctaaaaaatc ttattttatt ttgacagatt caggtggaat tcaggaagaa gcgccatcat
    13261 ttaataagcc agttttagtt ttgcgaagtg ttacagaacg tcctgaaggt gttgaagctg
    13321 gcacattaaa agtcattgga acaaataagc aaaatgtgta tcaagctgca aaagaattaa
    13381 tcgatgatga gagattatat catcaaatgt ctgaagcatc taatccttac ggtgatggat
    13441 ttgcttcaga aagaatagtt aatcatatta agtattattt gaatttaatc actgaaaaac
    13501 caagcgattt ctgaggaaaa tagacttttt gtctaattgt gtctaaattg tgtctaacta
    13561 tgcttatttt tttacaacaa tttgaataga attgacacca caaatcgcct atattatcat
    13621 ttagtttgta tgagtggaac ggttttcatg attgatatca gtcacacgga ggcatataaa
    13681 atgagtcgtt tctacaacat ttttaaacag ttcattcaat attattttta tctaataata
    13741 atattgggag gattatacct ttatacacac catgcattta tcttaggatt aatcattggt
    13801 gttactggtt ctgcaatcaa cacatgtatt tttgaatgct atttagctaa agctaaaaga
    13861 ccagacacta tgcatatttc aactggaaat atgtggcgat acttagttgc aattattgcc
    13921 tgtatgattt ggtaccttaa taaagcgcat gtaagtatca tcggtataat tattggttta
    13981 atgatttcat atgttgtagt tatcatacgt cctttactaa aggtgagcaa ataaattaag
    14041 aaagaggtga gattatggat cacaaatccc cgctcgtgag ttggaattta ttcggttttg
    14101 atatcgtttt caatttatca agtatattga tgatacttgt tacggcgttt cttgtttttc
    14161 tacttgctat catttgtacg cgtaatttga aaaaaagacc aactggcaaa caaaatttcg
    14221 ttgaatggat ttttgatttc gtgaggggaa tcattgaagg taacatggct tggaaaaaag
    14281 gtggtcaatt ccacttctta gcagtaacgc tgattctgta catttttata gctaatatgt
    14341 taggtcttcc gttttctata gtaacgaaag atcacacatt gtggtggaaa tcaccgacag
    14401 ctgatgcaac agtgacttta acgttgtcta caacgataat actgttaact cacttttatg
    14461 gaattaaaat gcgtggtacg aaacaatatc ttaaaggtta tgtacagccg ttttggccat
    14521 tggcaattat taatgttttt gaagagttca cttcaacatt aacgcttggt ctgcgtttgt
    14581 acggtaacat atttgcaggt gagatactat taacattact tgctggctta ttctttaacg
    14641 aaccagcatg gggttggatt attagtatcc caggattaat tgtttggcaa gcattttcaa
    14701 tatttgtagg aacaatccaa gcatatatct ttattatgct ttcgatggtt tatatgtcac
    14761 ataaagtggc agatgaacac taaaaatttc aataattata tacaatcaca ggaggaaatt
    14821 aaattatgaa tttaatcgca gcagcaatcg caattggttt atcagcatta ggagcaggta
    14881 tcggtaacgg tttaatcgtt tcaagaacag ttgaaggtgt agcacgtcaa ccagaagcac
    14941 gtggtcaatt aatgggtatc atgttcattg gtgtaggttt agttgaggca ttacctatca
    15001 tcggtgtagt aattgcattc atgacatttg ctggataatt aacagataaa agaggtcggg
    15061 acaaagcgca taggacataa ttcatgatgc atatatagta atatctttga actttattaa
    15121 atagttgaga tatgaacgca ccatgcctat cgcataaatt cagtaggtcc taacctcgtc
    15181 gtttttttct atataacact agcgattatt ttaatgaaag gagtgtcatg aacccgtgac
    15241 tgaaacagct aacttattcg ttcttggtgc agctggaggc gttgagtggg gtactgtgat
    15301 tgtacaggtc ctaactttca tcgtgttact tgcgttactt aaaaagttcg catggggtcc
    15361 attgaaagat gtaatggata aacgtgaaag agatattaac agagatatcg atgacgcaga
    15421 acaagctaag ttaaatgcac agaaacttga agaagaaaat aaacaaaaac ttaaagaaac
    15481 acaagaagaa gttcaaaaga ttttagaaga tgctaaggtt caagcacgtc aacagcaaga
    15541 acaaattatt catgaagcaa acgtacgtgc aaacggtatg attgaaacag cacaaagtga
    15601 aatcaatagc caaaaagaac gtgccattgc agatattaat aatcaagtat ctgaactatc
    15661 agtgttaatt gcttctaaag ttcttagaaa agaaatttct gaacaagacc aaaaagcatt
    15721 ggttgacaag tatctaaaag aggcaggcga taaataatgg taaaagtagc taacaagtat
    15781 gctaaagcat tatttgacgt gtcattagat acaaataatt tagagactat taatgaagaa
    15841 ttaacagtta taaatgaagc agtaaaagat aaaattgagc aattgagaat ggttgacagt
    15901 aatccaactc aaacagcaga acaacgtcgt gagttgatta atggtgtatt tactgacatt
    15961 aatccataca ttaaaaatat gatgtacgtg ttagcagata atagacatat ctcattaata
    16021 gctgacgtat tcaaggcgtt ccaaagctta tataacggac actacaatca agattttgca
    16081 acaattgagt caacatatga attgagtcaa gaagagttag ataagattgt caaactagta
    16141 actcaacaaa cgaagttatc taaagttatt gtagatacaa aaattaatcc agatttaatt
    16201 ggtggattta gagttaaagt cggcacaact gtattagatg gtagtgttag aaatgatctt
    16261 gtccaattac aaagaaaatt tagaagagtt aattaattat aaagaggagt gacatagatg
    16321 gccataaaag ctgaagaaat cagtgcatta cttcgctcac aaattgaaaa ttatgagtca
    16381 gaaatgtccg taactgatgt aggtactgta ttacaaattg gtgatggtat tgcattaatt
    16441 cacggattaa atgacgttat ggctggtgag ctagtagaat tccataacgg cgtacttggt
    16501 ttagcccaaa accttgaaga gtcaaacgtg ggtgtggtta ttttaggacc atacacaggt
    16561 attactgaag gtgacgaagt taaacgtact ggtcgtatca tggaagtacc agtaggtgaa
    16621 gaactaatcg gaagagttgt taatccatta ggacaaccta ttgatggaca aggaccgatt
    16681 aacacaacta aaacacgtcc agtagagaaa aaagctactg gtgtaatgga tcgtaaatca
    16741 gtagatgagc cattacaaac aggtatcaaa gcaattgatg ctttagtacc aattggtaga
    16801 ggtcaacgtg agttaatcat cggtgaccgt caaacaggta aaacaacaat tgcaattgac
    16861 acaattttga accaaaaaga tcaaggtacg atttgtatct atgttgctat tggtcaaaaa
    16921 gattcaacag taagagcaaa tgttgaaaag ttaagacaag caggcgcttt agactacact
    16981 attgttgtag cagcatcagc ttctgaacct tctccattat tatatattgc accatattca
    17041 ggtgtaacaa tgggtgaaga attcatgttt aacggtaaac atgttttaat cgtttatgat
    17101 gatttaacta aacaagcagc agcttatcgt gagttatcat tattattacg tagacctcca
    17161 ggtcgtgaag catacccagg tgacgtattc tacttacata gtagattatt agaaagagca
    17221 gcaaaattaa acgatgactt aggtggcggt tcaattactg cattaccaat tatcgaaaca
    17281 caagctggtg atatttcagc ttatgtacca acaaacgtta tttcaattac agatggacaa
    17341 atcttcttac aatctgattt attcttctca ggtgtaagac cagcgattaa tgccggacaa
    17401 tctgtatctc gtgttggtgg atctgcacaa attaaagcaa tgaagaaagt tgctggtacg
    17461 ttacgtcttg acttagcgtc atacagagaa cttgaatcat ttgcacaatt cggttcagac
    17521 cttgatgaat ttactgcaag taaattagaa cgtggtaaac gtactgttga agtcttaaaa
    17581 caagatcaaa acaaaccatt accagtcgaa caccaagtgt tgattattta tgcattaaca
    17641 aaaggatatt tagatgatat tccagttgta gatatcacac gttttgaaga cgagttaaac
    17701 cactgggcag aatcaaatgc tactgaactg ttaaatgaaa tcagagaaac tggtggctta
    17761 ccagatgctg agaagtttga cacagcaatt aacgaattca aaaaaagctt tagcaaatct
    17821 gaataataaa caagtttagt ataaggtggt gagatagtgg cttctcttaa agaaatagat
    17881 actcgaataa aatcaaccaa aaaaatgaag cagattacga aagcgatgaa catggtatca
    17941 agttcaaaac ttcgtagagc tgaaaaaaat acaaaacaat tcacaccata tatggataaa
    18001 atgcaagatg caattactgc agttgcaggg gcaagtagca atacaaacca tccaatgcta
    18061 agacctcgta aaattactag aagtggatat ttagttatca cgagtgataa aggtttagca
    18121 ggtgcatata gtgcaaacgt gcttaaaaaa ttgattactg atattgaagc gaaacatcaa
    18181 gatagtagcg aatacagtat tgtagtttta gggcaacaag gtgttgattt cctaaaaaat
    18241 agaggttatg acattgagta ttctcaagta gacgtacctg atcaaccttc tttcaaatct
    18301 gttcaagcac tagctaacca tgctatagac ttatacagtg aagaagaaat tgatgaatta
    18361 aatatatact atagtcatta tgtcagcgtt cttgaaaaca agcctacatc tagacaagta
    18421 ttaccattat ctcaagagga ttctagtaag gggcatggtc atttgtcttc ttatgaattt
    18481 gagccagata aagaatctat cttaagtgta atcttgcctc aatatgttga gagtttgatt
    18541 tacggaacaa tattagacgc aaaagcaagt gagcatgcaa cacgtatgac tgcgatgaaa
    18601 aatgccactg ataatgcaac tgaacttatt gatgacttat cattagaata taacagagcg
    18661 agacaagcag aaattacgca acaaattact gaaattgttg gtggttccgc agcgcttgaa
    18721 taatatttaa aggaggaaaa tagcatggga attggccgtg taactcaagt tatgggtcct
    18781 gtaattgatg ttcgatttga acataacgaa gttcctaaaa ttaataacgc cttggttatt
    18841 gatgtgccta aagaagaagg tacaatacaa ctaacattag aagttgcgct gcaattaggt
    18901 gacgacgttg ttcgtacaat tgcgatggat tcaactgatg gtgtccaaag aggcatggat
    18961 gtaaaagata caggcaaaga aattagtgta cctgttggtg acgaaacatt aggtcgtgta
    19021 tttaatgtac taggtgaaac aattgacctt aaagaagaaa ttagtgattc tgttcgccgc
    19081 gatcctatcc atcgtcaagc accagcattc gatgaacttt caacagaagt tcaaatttta
    19141 gaaacaggta ttaaagtagt agatttacta gcaccttata ttaaaggtgg taaaatcgga
    19201 ttgttcggtg gtgccggtgt aggtaaaaca gtattaatcc aagaattaat taacaacatc
    19261 gctcaagagc acggtggtat ttctgtattc gccggtgtag gtgaacgtac tcgtgaaggt
    19321 aacgatttat acttcgaaat gagtgacagt ggtgtaatta agaaaacagc catggtattc
    19381 gggcaaatga atgagccacc tggtgcacgt atgcgtgttg cattatctgg tttaacaatg
    19441 gctgaatatt tccgtgacga acaaggtcaa gacgtattat tattcatcga taacattttc
    19501 agatttacac aagctggttc tgaggtatct gcattattag gtcgtatgcc ttctgcagta
    19561 ggttaccaac caacacttgc tactgaaatg ggacaattac aagaacgtat tacgtctaca
    19621 acaaaaggat cagttacttc tattcaagcg gtattcgtac ctgccgatga ctatactgac
    19681 ccagcgcctg cgacagcgtt tgcccattta gatgcaacta caaacttaga acgtaaatta
    19741 actgaaatgg gtatttatcc agccgtggat ccattagcgt ctacatcaag agcattggaa
    19801 ccatcaattg taggtcaaga acattatgaa gtagctcgtg atgtacaatc aacacttcaa
    19861 aaataccgtg aattacaaga tatcattgct atcttaggta tggacgaatt atctgatgaa
    19921 gataaacaaa cagttgaacg cgcacgtaga attcaattct tcttatctca aaacttccac
    19981 gtagcggaac aatttactgg tcaaaaaggt tcttatgtac ctgttaagac aacagttgca
    20041 aactttaaag atatcttaga tggtaaatat gaccatattc cagaagatgc attccgttta
    20101 gttggtagca tggatgatgt tattgcaaaa gctaaagata tgggtgttga agtataacaa
    20161 ttaggaggaa tggataatga atacattaaa cctagatatt gtcactccta atggttctgt
    20221 ttacaatcgt gataatgttg aactcgttgt tatgcaaaca acagctggtg agataggtgt
    20281 catgagtgga catattccaa ctgtagctgc tttaaaaaca ggctttgtaa aagtgaaatt
    20341 tcacgatgga actgaatata ttgctgtaag cgatggcttt gttgaagtta gaaaagataa
    20401 agtttcaatc attgttcaga ctgcagaaac tgcaagagaa attgatgttg aaagagctaa
    20461 attagccaaa gcaagagcag agtctcactt ggaaaatgat gacgacaata ctgatattca
    20521 tagagccgaa agagctttag agagagcaaa taaccgtttg cgtgtggctg aattaaaata
    20581 gtaaataaag ggtcgaagat gtgatttcat atcttcgacc cttttttgaa ttatattgat
    20641 ttaaagatac aaaacatgag aggggggaag gaattgataa agaaccatta aagatttatg
    20701 atgtagtggt tctttatcat taaacacagc taatgtgtat ttaaaaatag gaatacatga
    20761 gtaaaactca tgtataagaa atactaattt ctaaagaaaa agtatttctt tatgttgggg
    20821 ccccgtcaac tactgccaaa tacaacacta tagagtctag acattgattt atgtccgact
    20881 cccaagaata gttttacttt tttacaatca ctaatagatt gctaaaatca aaatttcctt
    20941 caccactatc tacagtcgac atttcatttt ttgaaattat ctacattttt tcataccaag
    21001 atattttata gttatgatat ttatgtaaaa agaattatat agtaagttag cttaaacttt
    21061 actaaaaacg ggtattaaac tttgtatcat tatttaaatt tttcatgtac aatgtaatac
    21121 agtaatctta tgaggtgata aaatggatta tatcggacaa tatgcagtta tccatttagt
    21181 gttacatgtt gtatgtattt gtattgccta ttgggcttta caatcaatta gattagatca
    21241 attttttaaa aaaggatacg ccactcaatt acaagtgtgt atgatatttg ttgctatttt
    21301 attaggcact gcagtaagca attttattgt agatttgtta caatactcga cgcaggtaaa
    21361 atatttaata aaataagtct aactctatga tttgtaatca aaactagata taattaaata
    21421 atgacttaaa ataattttaa aatagggaaa tgtaaagtaa taggagttct aagtggagga
    21481 tttacgatgg ataaaatagt aatcaaaggt ggaaataaat taacgggtga agttaaagta
    21541 gaaggtgcta aaaatgcagt attaccaata ttgacagcat ctttattagc ttctgataaa
    21601 ccgagcaaat tagttaatgt tccagcttta agtgatgtag aaacaataaa taatgtatta
    21661 acaactttaa atgctgacgt tacatacaaa aaggacgaaa atgctgttgt cgttgatgca
    21721 acaaagactc taaatgaaga ggcaccatat gaatatgtta gtaaaatgcg tgcaagtatt
    21781 ttagttatgg gacctctttt agcaagacta ggacatgcta ttgttgcatt gcctggtggt
    21841 tgtgcaattg gaagtagacc gattgagcaa cacattaaag gttttgaagc tttaggcgca
    21901 gaaattcatc ttgaaaatgg taatatttat gctaatgcta aagatggatt aaaaggtaca
    21961 tcaattcatt tagattttcc aagtgtagga gcaacacaaa atattattat ggcagcatca
    22021 ttagctaagg gtaagacttt aattgaaaat gcagctaaag aacctgaaat tgtcgattta
    22081 gcaaactaca ttaatgaaat gggtggtaga attactggtg ctggtacaga cacaattaca
    22141 atcaatggtg tagaatcatt acatggtgta gaacatgcta tcattccaga tagaattgaa
    22201 gcaggcacat tactaatcgc tggtgctata acgcgtggtg atatttttgt acgtgatgca
    22261 atcaaagaac atatggcgag tttagtctat aaactagaag aaatgggcgt tgaattggac
    22321 tatcaagaag atggtattcg tgtacgtgct gaaggggaat tacaacctgt agacatcaaa
    22381 actctaccac atcctggatt cccgactgat atgcaatcac aaatgatggc attgttatta
    22441 acggcaaatg gtcataaagt cgtaaccgaa actgtttttg aaaaccgttt tatgcatgtt
    22501 gcagagttca aacgtatgaa tgctaatatc aatgtagaag gtcgtagtgc taaacttgaa
    22561 ggtaaaagtc aattgcaagg tgcacaagtt aaagcgactg atttaagagc agcagccgcc
    22621 ttaattttag ctggattagt tgctgatggt aaaacaagcg ttactgaatt aacgcaccta
    22681 gatagaggct atgttgactt acacggtaaa ttgaagcaat taggtgcaga cattgaacgt
    22741 attaacgatt aattcagtaa attaatataa tggaggattt caaccatgga aacaattttt
    22801 gattataacc aaattaaaca aattatacct cacagacagc catttttatt aattgataaa
    22861 gtagttgaat atgaagaagg tcaacgttgt gtggctatta aacaagtatc aggaaacgaa
    22921 ccattctttc aagggcattt tcctgagtat gcggtaatgc caggcgtatt aattactgaa
    22981 gcgttagctc aaacaggtgc ggtagctatt ttaaatagtg aagaaaataa aggtaaaatc
    23041 gctttatttg ctggtattga taaatgtcgt tttaaacgtc aagtagtacc tggtgatact
    23101 ttaacgttgg aagtagaaat cactaaaatt aaaggaccaa taggtaaagg taatgctaaa
    23161 gctactgtcg atggtcaact tgcttgtagt tgtgaactta catttgcaat tcaagatgta
    23221 aaataaaaca aaaaaaacat tcaaagattt aatgtgttgg cataatcttt gaatgttttt
    23281 tattttactc ttctaatttt tcatccttta actttggttt agactgcatc attcgattaa
    23341 atgatttttt taattcttca ccagataatc catcatcaat aagttgttct aataaacttt
    23401 cagcatactg ttgacgtaaa gtgtaaatat gacattcaaa tacgatagac attgttgttt
    23461 taaaattttt aatacttttc acagtagatt caaataaagc tggatcattg gctggacgcg
    23521 tgatgacgac acgaatatcc aataactgtt cattgtcttg atattcctta aatgtttcat
    23581 aatgctcatt agaaatgaca atttcaagca accaacccgt gccactttcc tctttattaa
    23641 ttattacgcc atcttctaat tcatattcta taattctgcc gtgttcattc acaatttgaa
    23701 accttactgc tttaaaagtt ttcatattcc cacatcccat tagtttaata tttatgattt
    23761 ttgaattatt tagaagattt aaggtcattt catgactaat ttctttaaat ataacaaata
    23821 acgagatgct atagctatta attatataaa atttgccatt ttgaccattt ttaagctagt
    23881 aattaagata taggcatagg aggtgaatca cacactacat gctaaataaa atcgtaattg
    23941 tcgggagact gacgaaagac gcacaaatat ttgaaaagga ggatagaaaa attgcaacgt
    24001 tttgtgttgc aacgcaccga aattataaag atgaaaatgg agaaatcgtc tgtgattact
    24061 tattctgtaa agcatttggc aagttagctt ctaatataga aaaatatact aatcaaggta
    24121 cattggttgg tataactggt caaatgagat caagaaagta tgataaagac ggacaaacac
    24181 actttgtcac tgaattatat gttgaaacaa taaaatttat gtcccctaaa tcccaaaata
    24241 atgaaattct ctcagatagt atttagatat tgactctcaa aatatagata atcatgactt
    24301 attagaaatt taaatccaat tatcctaaac atccttaata tacatttaat ccatcctaaa
    24361 ttttttatag atctaattaa cttgaaatat actcacttaa aaacgactta catgactagt
    24421 actgttgtgt aggtcgtctt tttgtgtagt ttttctaata gaaaatagac atagattgca
    24481 aaacgattac ataaaaaatt gcaaataaaa aaacattttt actgtcatga caattctcga
    24541 tagaatcata tctgaaatta tgggggattt cgttgtaatc actgtgtaaa aaacaaggct
    24601 atttttttat tttgttgtaa cctgcacgga ttttatagat gttacagtag tctctgtaaa
    24661 acttttagag aaacaaatta cttgtaggag gattttaaga ttatgaagaa aacattactc
    24721 gcatcatcat tagcagtagg tttaggaatc gtagcaggaa atgcaggtca cgaagcccat
    24781 gcaagtgaag cggacttaaa taaagcatct ttagcgcaaa tggcgcaatc aaatgatcaa
    24841 acattaaatc aaaaaccaat tgaagctggg gcttataatt atacatttga ctatgaaggg
    24901 tttacttatc actttgaatc agatggtaca cactttgctt ggaattacca tgcaacaggt
    24961 actaatggag cagacatgag tgcacaagca cctgcaacta ataatgttgc accatcagct
    25021 gttcaagcta atcaagtaca atcacaagaa gttgaagcac cacaaaatgc tcaaactcaa
    25081 caaccacaag catcaacatc aaacaattca caagttactg caacaccaac tgaatcaaaa
    25141 tcatcagaag gttcatcagt aaatgtgaat gctcatctaa aacaaattgc tcaacgtgaa
    25201 tcaggtggca atattcatgc tgtaaatcca acatcaggtg cagctggtaa gtatcaattc
    25261 ttacaatcaa cttgggattc agtagcacct gctaaatata aaggtgtatc accagcaaat
    25321 gctcctgaaa gtgttcaaga tgccgcagca gtaaaattat ataacactgg tggcgctgga
    25381 cattgggtta ctgcataagc catttatgca tagctaatta atagttatat aagtaacttt
    25441 tagattggaa tatatcgggg aattctcgat atgttccggt ttttttattt cgcaaatata
    25501 tgtcattata gaattattat ttaaagataa taatacaact gagcaacaat aattgaaaat
    25561 gttgaaaccg tttgctaaga ttaatttgca attgaatact aaggaactac taggggagcc
    25621 taatgatatg gctgagatga attgttcaga cccttatgac ctgatttggt tagtaccaac
    25681 gtaggaaagt agttattgaa gtgatggggc tagtatagca acattgccat caaactactt
    25741 tcatgttgga agtagttttt ttatttaggg aggatttttt acaatggaat tttcacaaaa
    25801 attgtaccaa gctgcaaagc caatcattaa tgatatttat gaggatgact ttattcagaa
    25861 gatgttatcc ggagatatag gagctgacgc attacgccat tacttaaaag ctgatgctgc
    25921 gtatttaaaa gaatttacaa acttatatgc tttgttaatt ccaaaaatga atagcatgaa
    25981 tgacgtaaaa tttttagtag agcaaataga atttatggtt gaaggtgaag tacttgcgca
    26041 tgatatttta gcgcaaattg ttggtgaatc atacgaagaa atcattaaaa caaaagtttg
    26101 gcctccaagt ggcgaccatt acataaaaca tatgtatttc caagcgcata gtcgtgaaaa
    26161 tgctatttat acaattgctg caatggcacc ttgtccatat atttatgcag aattagctaa
    26221 gcgctctcaa agtgatcata agcttaatag agaaaaagat acggcaaaat ggtttgattt
    26281 ttatagtact gaaatggatg acataattaa cgtatttgag gcattaatga ataaattagc
    26341 cgaatcaatg tctgacaagg aattggaaca agtaaagcaa gtatttttag aaagttgcat
    26401 acatgagcgt cgatttttca atatggctat gacactagaa caatgggaat ttggaggaaa
    26461 agtaaatgat taaacctaaa atagcattaa ccattgcagg tactgatcca acaggtggtg
    26521 ccggcgtaat ggctgattta aaatcatttc attcatgtgg tgtatatggt atgggcgtcg
    26581 ttacaagtat tgttgctcaa aatacattgg gcgtacaaca tattcataat ttaaatcatc
    26641 aatgggtaga tgaacaactt gatagtgtct tcaatgatac cttacctcat gctattaaaa
    26701 cggggatgat tgctacagca gatactatgg aaacgattcg tcattattta atgcaacatg
    26761 aatctattcc atatgtaatt gatcctgtta tgttggcgaa aagcggtgat tcattaatgg
    26821 ataatgacac aaagcaaaac ttgcagcata cgttattgcc attagctgac gtagtaacac
    26881 cgaatttacc agaagctgaa gaaataacgg gactaaccat tgatagtgaa gaaaaaatta
    26941 tgcaggctgg ccgcatcttt attaatgaga ttggtagtaa aggtgtcatc attaaaggcg
    27001 gtcattcaaa tgatactgat atagcaaaag attatttatt tactaacgaa ggtgttcaaa
    27061 catttgaaaa tgaacgattt aaaacaaaac atacgcatgg aacagggtgt acattttcag
    27121 cagttataac ggcagaactt gcaaaaggta gaccattatt tgaggctgta cacaaggcta
    27181 aaaagtttat ttcaatgagt atacaatata cgcctgaaat cggccgtggt agaggtccag
    27241 tgaatcattt tgcatattta aagaaagagg gattagacga tgaattatct aaataaaata
    27301 cgtattgaaa acccattaac aatttgttat acaaacgatg tagttaaaaa ttttacagcg
    27361 aatggtttat taagtattgg tgctagccct gcaatgagtg aagctcccga agaagctgaa
    27421 gaattttaca aagttgcaca agcgctatta atcaatatcg gtactttaac agcagaaaat
    27481 gaacaagata ttattgcgat tgctcaaacg gcaaatgagg caggcttacc tattgtattt
    27541 gaccctgtag ctgttggtgc ttctacatat cgaaagcaat tttgtaaatt attattgaaa
    27601 tcagcgaaag tatcagtaat taaaggcaat gcatctgaaa tattagcgtt gattgatgat
    27661 acagcaacta tgaaaggtac agatagtgat gctaatcttg atgcggttgc aatagcgaaa
    27721 aaggcttacg caacatataa aactgcaata gtaatcacag gtaaagagga cgttattgtt
    27781 caagataata aagccttcgt attagctaat ggatctccat tattagcacg agtaactgga
    27841 gctggttgtt tattaggagg cgttattgct ggatttttat ttagagaaac agaaccagac
    27901 atagaagcgt taattgaagc ggtaagcgta tttaatattg ctgctgaggt agctgctgaa
    27961 aatgaaaatt gtggtggtcc tggtacgttt tcaccattgt tgcttgatac gttatatcat
    28021 ttaaatgaaa caacctatca acaacggatt cgtattcaag aggtggaata atatgtttaa
    28081 ccaatcgtat ctaaatgtgt attttatttg tgggacctcc gatgttccga gtcatcgaac
    28141 tattcatgaa gtattagaag cagcattaaa ggcagggatt acacttttcc aatttcgaga
    28201 aaagggtgaa tcagctttaa aaggaaacga taaacttgta ttagctaaag aattacagca
    28261 tctctgtcat caatatgatg tgccttttat cgttaatgat gatgtatcct tggcaaaaga
    28321 aattaacgcg gatggtatac atgtcggtca agatgatgcg aaagtcaaag agatagcaca
    28381 atatttcact gataaaatta ttggacttag tattagtgat ttagatgagt atgcaaaatc
    28441 tgatttaact catgttgatt atataggtgt tggaccaatc tatcctacgc cgtcgaagca
    28501 tgacgcgcat ataccagtag gtccagaaat gattgcaacg tttaaagaaa tgaatccgca
    28561 actaccgatt gtagctattg gcggtatcaa tactaataat gtggcgccga ttgtagaagc
    28621 aggtgccaat ggaatttcag tcatttcagc catttctaaa agtgaaaata ttgaaaagac
    28681 tgttaatcga ttcaaagatt tttttaataa ttagttaaat ttttccactt attgtttcat
    28741 acgtactcat taatgtgata gaataaatcc tatgtgaata taatgataga ggtggaaaaa
    28801 tgaagaaaaa agcgttacta ccattatttt taggtattat ggtctttttg gctggttgtg
    28861 actattctaa acctgaaaaa cgtagtgggt ttttctacaa tacattcgta gatccaatga
    28921 aaaatgtatt ggattggttg ggaaataact tattaaacga caattatggt ttagctatta
    28981 ttatccttgt attggtaatt cgtattattt tattaccatt catgttgtca aactataaaa
    29041 atagtcatat gatgcgtcaa aaaatgaaag ttgcaaagcc agaagttgaa aaaattcaag
    29101 aaaaagtgaa acgtgcgcgt acacaagaag aaaaaatggc tgcaaaccaa gaattaatgc
    29161 aagtatataa aaagtatgac atgaacccga ttaagagtat gttgggttgt ttaccaatgc
    29221 taatccaatt accaatcatc atgggattat actttgtact taaagaccaa cttgtagatg
    29281 gtttgtttaa atatccacac ttcttatggt tcgatttagg acgtcctgat atttggatta
    29341 caattattgc cggtgtttta tactttatcc aagcatatgt atcaagtaaa acgatgccag
    29401 acgaacaacg tcaaatgggt tacatgatga tggtcatttc accaattatg attatctgga
    29461 tttcattaag ctcagcatca gcacttggtt tgtactggtc agtcagtgcg gcgttccttg
    29521 tagttcaaac acactttgcg aacatttatt atgaaaaagt cgctaaaaaa gaagtacaac
    29581 ctttcattga agcgtatgaa agagagcaca acggcggcag caataaaaaa ggcaaaaaca
    29641 cacaagttgt gtctaaaaag aaaaagaaat aaccttagca agtccaaata atgtgcaccc
    29701 taaaagttga agataatttt aactttatag ggcgcacatt atttttcgct tcaatagcga
    29761 aacttagtgt cacattatac aatatgtgat aaatagaaac agcagtaaga tgatttcaaa
    29821 tgtgaaagtc atcctactgc tgtttttatt tgaaataata ttaacgagtt ttcagtatgt
    29881 aaaaagtggt taataacaga gttgtcattc ttcatcgcaa tgtcatcttg acacaatgat
    29941 tcatcagtgt agcagtatat gtcgcaaatt tccacagcaa ttcacttata catgtaacag
    30001 tgtgaaataa taacgtcaca acggcataat acacattgca acgtcccaac aacatatcat
    30061 agaatcgtaa cggcatgaca tcaacaccaa acctggctat aacaccataa cagcgcaacg
    30121 ccacatcata gcactacatc aagtcgcatc gtagcaccag cacatcaaag taacatggca
    30181 tcaacatcaa accggtctat aacaccataa caccgcaatg ccacatcata gcactacatc
    30241 aagtcgcatc gtggtaccag cacatcaaag taacatggca tcaacgccaa acctgtctat
    30301 aacaccataa caccgcaatg ccacatcata gcactacatc aagacgcatc gtggtaccag
    30361 cacatcaaag tagcatgaca tgtcaccaag ctcccacaac aacacatcaa agtaacatgg
    30421 catgacacca agcttccaca acaacacgcc attacatcgc atcatcatac tacaacttca
    30481 atgtctagtc gtgacaattc cattccgtaa aaaactgttt caagtacatc atcataaagt
    30541 cgtgacgttc tttagcaatc atcttcgccg tatctgtatg cattaaagat tctaacttaa
    30601 gtaatttttc aaagaaatgt ttaattgcag atggtggcaa ctgttcaact aaatcatcat
    30661 taatcttatc tagtgacata tgttctgtcc acataggttc accaaagtgt cctgcaaatt
    30721 gaaatgttcg tgcaacacct atagcgccta tagcatcaag acgatctgca tccctgacaa
    30781 tttgaccttc taaagataaa gtgacatgat catttttgcc attgcgatag ctcatattat
    30841 taataataaa taaaatgtgc tcttgatctt cggttgatag tgataaagaa gataaaaatg
    30901 acttcaattc aacatattgt ttgttagcat ctacaacttt ttcgtcaacg gtatcatgaa
    30961 gcaaacatgc gagttcaatg actaaagtat cattaacacc ttcattttca gcgattgatt
    31021 tagctaaagc agtgacacga tatacatgcg caatatcatg gcctgtataa tcattttgat
    31081 gaatagattc catatatttt ttggcagcac taatgacgtc tgattgttgc attaaagtta
    31141 cactcctcat atttctattt tataagatag gtgacaataa ttgtgaaata ccttctttaa
    31201 atttaatcca aagacttcgc ttagcataac gtgcttttgt taattcagaa gatactgcta
    31261 aatcatctat aaaagcttgt tttaattttt tggcaatttg ttggtcataa ataaaagcgt
    31321 tgacttcgaa attcaatgtg aaactgcgat ggtccatatt agctgttccc acacttgcaa
    31381 tttcatcatc tataacaagt gtttttgagt gtaaaaagcc attgtcgtag tgaaatactt
    31441 taacaccggc atctaataag gatgctgcat tttttaaagt agcccaaaaa acaaacggat
    31501 ggtcaggttt attaggaatc atgatattga catcaacacc acctaatgcc gcaattttaa
    31561 tagaatctaa aaaggcttga tcaggtatga aatagggaga ttgaatataa atcgattttt
    31621 tagcagatga aatcattttc aaatagccgt atttaatctg ttcccattct tcgtcaggac
    31681 cactagaagc tatttgaacg ccaattgttc caccagaatt tacatctggg aaataacgat
    31741 catcatagga gatgtggtca cgtgtggctt gtgaattcca atctagaata aatcgtaatt
    31801 gcaatgcatt cactgcatcc ccgacaattc gtaaatgcgt atctcgccaa tagccgaatt
    31861 tttttgattt acctaagtac tcatcaccaa cattaaaacc accaacatat ccaatttgcc
    31921 catctattac aacaattttt cgatggtttc gattgttcat acgcaagtta attaaaggta
    31981 attttgatgg gaaaaatgct tcagcatgtc cacctttatt gcgaaacggg cgtaagcctt
    32041 ttttacgcag tccacgagaa cccatgtcat cataaagaat tttaacttct acaccttgtt
    32101 ccgctttttt accaagttca tttaaaatgg tacgacctaa ttcatcattt tgaataatat
    32161 agtactgaaa atgaatataa tcagtagcat tacggatgtc ttgtattagg tcatcaaatt
    32221 tttcttggcc gtctgtgtat atttttaaat cgttgtctgt tgttaaaaat gctgcgttat
    32281 tatataacaa catttgaatc atttctttaa attttacaat ttgataattg gaatttgaaa
    32341 agttttcatt ttttaaagca gctaattgct catcaacgat taactctaat ccttttttat
    32401 cttccttatc aattttgaaa atttggtcac gttgaatttg tcgtcctaat agtaagtata
    32461 aaatgaagcc gaataaaggc aagaaaacta agactagtaa ccaagcccag atagaattgg
    32521 cagaacgtct ttccatgaaa ataatggtaa aggcgaataa taagtttaaa ataaatgcac
    32581 caataaatat tgaatttaaa ataatattag aatgcttgag tgcaatggat aataactcta
    32641 tcatttgaaa cctcccatcg aaaatcttaa acgagtagta tatcttatta attgtatcat
    32701 gaagcaattt aaatatcatg acaatttagt ttgaaaaaca cactttgtgt tgacgttgtg
    32761 ttttatggga tatagtatag taaggaatgt aaatgaagga gtgaatgcta tgactgaaca
    32821 agataatgca catcattctg aacaaataaa aacgaatctt aaatcacgtt taaatcgaat
    32881 tgaaggacaa gtgagagcga ttaatcgcat gattgaagaa gatgtctatt gtgatgatgt
    32941 ccttacgcaa ataagagcga cacgttcggc gttaaacagt gttgcgataa agttattaga
    33001 acaacatatg aaaagttgta ttatgaataa agttaatcaa ggtgctcagg aagaggcaat
    33061 ggaagagtta ttagtgactt ttcaaaaatt gattaaagac taaaggagtt taagatgata
    33121 catcaaaata cgatttacac agcgggaatt gaaacagaag aacaagtaag tcaattgaca
    33181 gaacgcattt caaatatgat aggtgttcat caagtgaata ttaatataat agatggtcaa
    33241 gtaactgtat cgtatgagac accagcaaat ttgaatagta ttgaaaaaga aatctatgat
    33301 gaaggataca aaattgtatt ttagggtata atgtagagtg cgctatagat tttaattttg
    33361 aaaataaatt aaaaattttg taaatgatgt agtaaaggta tgtcgaatag ggtaagataa
    33421 tattgaaagc gatccaaacg aggtgataat aatgaaacgt cctgaaaaga ttcaaaatgt
    33481 agtcaaacta ttgtcatcat taggtgtgaa tattaaaaaa actaaatctc gtttagacat
    33541 tattaatact ttgccagcat ctaataaagt aagtcacgaa ttaaaataat tgactgtgat
    33601 tgtgaatcac acaccgttaa agctatcgtc ggactattcg atgatagctt ttttgactgg
    33661 atttactaaa atgaaagcaa gtactatata acaaaatccc tctttagagt gtacatttta
    33721 caatgtgtac ttcaaagagg gattgttatt atcgagtaat atctatatat gacattttaa
    33781 atcgacgacg taaatagact atataacgtc atcttgaaaa gttatgatat ttgaaactca
    33841 aaatagttta aattaattac tttttggatg gtataaatcg acatatcgtt ttggttcatg
    33901 ataataaatt gataagacta tacctattcc agtcatcata ctccatagcg cactaccacc
    33961 ataactaata aatggtaatg gaataccagt gattggtaac aactgaattg tcataccaat
    34021 attttgtaaa atatggaaca caagtaaagt gacgaaacca acgataaaga ttttgttaaa
    34081 ttgatcttca attttcgcag ctaatcttat tagatggaag attaaaaata aaaatattaa
    34141 gatcaatatg acagaaccga taaagccaag ttcctctcca atcactgaaa agataaagtc
    34201 agtatgattt tcaggtatat aaacttcacc gtgattgtat cctttaccta gtaactgtcc
    34261 agaaccgata gctttaagtg attcagttaa atgatagcca tcaccactac tatatgtata
    34321 ggggtcaagc catgaattga ttcgtcccat ttgatacagt tggacaccta ataaattttc
    34381 aattaatgcg ggtgcatata gaatacctaa aatgactgtc attgcaccaa caatacctgt
    34441 aataaagata ggtgctaaga tacgccatgt tataccactt actaacatca cacctgcaat
    34501 aatagcagct aatactaatg tagttcctag gtcattttgc agtaatatta aaatacttgg
    34561 tactaacgag acaccaataa ttttgaaaaa taataacaaa tcactttgga atgatttatt
    34621 gaatgtgaat tgattatgtc tagaaacgac acgcgctaat gctaaaatta aaataatttt
    34681 catgaattca gatggctgaa tactgatagg gccaaacgtg taccaacttt tggcaccatt
    34741 gataataggt gtaataggtg actcaggaat aacgagcaag cctattaata atagacagat
    34801 taagaaatac aataaatatg tataatgttt aatcttttta ggtgaaataa acatgatgat
    34861 acctgcaaaa attgcaccta aaatgtaata aaaaatttgt ctgataccga aattagcact
    34921 gtattgacca ccgcccattg ccgagttaat aagcagaaca ctgaaaattg ctaaaacagc
    34981 tatagtggct actaataccc agtctacttt gcgaagccaa tgcttatccg gctgttgacg
    35041 agatgaataa ttcattgcaa actcctttta tactcactaa tgtttatatc aattttacat
    35101 gactttttaa aaattagcta gaatatcaca gtgatatcag ctatagattt caatttgaat
    35161 taggaataaa atagaaggga atattgttct gattataaat gaatcaacat agatacagac
    35221 acataagtcc tcgtttttaa aatgcaaaat agcattaaaa tgtgatacta ttaagattca
    35281 aagatgcgaa taaatcaatt aacaatagga ctaaatcaat attaatttat attaaggtag
    35341 caaaccctga tatatcattg gaggaaaacg aaatgacaaa agaaaatatt tgtatcgttt
    35401 ttggagggaa aagtgcagaa cacgaagtat cgattctgac agcacaaaat gtattaaatg
    35461 caatagataa agacaaatat catgttgata tcatttatat taccaatgat ggtgattgga
    35521 gaaagcaaaa taatattaca gctgaaatta aatctactga tgagcttcat ttagaaaatg
    35581 gagaggcgct tgagatttca cagctattga aagaaagtag ttcaggacaa ccatacgatg
    35641 cagtattccc attattacat ggtcctaatg gtgaagatgg cacgattcaa gggctttttg
    35701 aagttttgga tgtaccatat gtaggaaatg gtgtattgtc agctgcaagt tctatggaca
    35761 aacttgtaat gaaacaatta tttgaacatc gagggttacc acagttacct tatattagtt
    35821 tcttacgttc tgaatatgaa aaatatgaac ataacatttt aaaattagta aatgataaat
    35881 taaattaccc agtctttgtt aaacctgcta acttagggtc aagtgtaggt atcagtaaat
    35941 gtaataatga agcggaactt aaagaaggta ttaaagaagc attccaattt gaccgtaagc
    36001 ttgttataga acaaggcgtt aacgcacgtg aaattgaagt agcagtttta ggaaatgact
    36061 atcctgaagc gacatggcca ggtgaagtcg taaaagatgt cgcgttttac gattacaaat
    36121 caaaatataa agatggtaag gttcaattac aaattccagc tgacttagac gaagatgttc
    36181 aattaacgct tagaaatatg gcattagagg cattcaaagc gacagattgt tctggtttag
    36241 tccgtgctga tttctttgta acagaagaca accaaatata tattaatgaa acaaatgcaa
    36301 tgcctggatt tacggctttc agtatgtatc caaagttatg ggaaaatatg ggcttatctt
    36361 atccagaatt gattacaaaa cttatcgagc ttgctaaaga acgtcaccag gataaacaga
    36421 aaaataaata caaaattgac taactgaggt tgttattatg attaatgtta cattaaagca
    36481 aattcaatca tggattcctt gtgaaattga agatcaattt ttaaatcaag agataaatgg
    36541 agtcacaatt gattcacgag caatttctaa aaatatgtta tttataccat ttaaaggtga
    36601 aaatgttgac ggtcatcgct ttgtctctaa agcattacaa gatggtgctg gggctgcttt
    36661 ttatcaaaga gggacaccta tagatgaaaa tgtaagcggg cctattatat gggttgaaga
    36721 cacattaacg gcattacaac aattggcaca agcttacttg agacatgtaa accctaaagt
    36781 aattgccgtc acagggtcta atggtaaaac aacgactaaa gatatgattg aaagtgtatt
    36841 gcataccgaa tttaaagtta agaaaacgca aggtaattac aataatgaaa ttggtttacc
    36901 tttaactatt ttggaattag ataatgatac tgaaatatca atattggaga tggggatgtc
    36961 aggtttccat gaaattgaat ttctgtcaaa cctcgctcaa ccagatattg cagttataac
    37021 taatattggt gagtcacata tgcaagattt aggttcgcgc gaggggattg ctaaagctaa
    37081 atctgaaatt acaataggtc taaaagataa tggtacgttt atatatgatg gcgatgaacc
    37141 attattgaaa ccacatgtta aagaagttga aaatgcaaaa tgtattagta ttggtgttgc
    37201 tactgataat gcattagttt gttctgttga tgatagagat actacaggta tttcatttac
    37261 gattaataat aaagaacatt acgatctgcc aatattagga aagcataata tgaaaaatgc
    37321 gacgattgcc attgcggttg gtcatgaatt aggtttgata tataacacaa tctatcaaaa
    37381 tttaaaaaat gtcagcttaa ctggtatgcg tatggaacaa catacattag aaaatgatat
    37441 tactgtgata aatgatgcct ataatgcaag tcctacaagt atgagagcag ctattgatac
    37501 actgagtact ttgacagggc gtcgcattct aattttagga gatgttttag aattaggtga
    37561 aaatagcaaa gaaatgcata tcggtgtagg taattattta gaagaaaagc atatagatgt
    37621 gttgtatacg tttggtaatg aagcgaagta tatttatgat tcgggccagc aacatgtcga
    37681 aaaagcacaa cacttcaatt ctaaagacga tatgatagaa gttttaataa acgatttaaa
    37741 agcgcatgac cgtgtattag ttaaaggatc acgtggtatg aaattagaag aagtggtaaa
    37801 tgctttaatt tcatagagat tagtcgaggg accttttact tataaaaatg atttgaatta
    37861 atactaaaag attacaaaga agaggtggtt ttgtgtgtaa atacaaaatt gcctttttct
    37921 ttttatgtta aatctataaa tttgaaacta aatcaaggtt aattctatgt acacacttta
    37981 tataggaagt agtttgaatg tttatataat gttttacaaa aagatgtagt attataatgt
    38041 ctaatttcac atgtgtttca gtaaaatttg ttgtggaatg ttaacgatat acgtatttta
    38101 taaaaaattt tttataatga ttattcgaat gatgcgtaac gcttacatct tatctaatgc
    38161 tagctttttg acaaaaatat gacaatcaat taatgtgatt ctaataaata ttcgcaaatt
    38221 gctttattgc gattaaattt ttttggtggt actatataga agttgatgaa atattaatga
    38281 acttatatgc aaaagtatat tgagaaataa acaggtaaaa aggagaatta ttttgcaaaa
    38341 ttttaaagaa ctagggattt cggataatac ggttcagtca cttgaatcaa tgggatttaa
    38401 agagccgaca cctatccaaa aagacagtat cccttatgcg ttacaaggaa ttgatatcct
    38461 tgggcaagct caaaccggta caggtaaaac aggagcattc ggtattcctt taattgagaa
    38521 agtagtaggg aaacaagggg ttcaatcgtt gattttagca cctacaagag aattggcaat
    38581 gcaggtagct gaacaattaa gagaatttag ccgtggacaa ggtgtccaag ttgttactgt
    38641 attcggtggt atgcctatcg aacgccaaat taaagccttg aaaaaaggcc cacaaatcgt
    38701 agtcggaaca cctgggcgtg ttatcgacca tttaaatcgt cgcacattaa aaacggacgg
    38761 aattcatact ttgattttag atgaagctga tgaaatgatg aatatgggat tcatcgatga
    38821 tatgagattt attatggata aaattccagc agtacaacgt caaacaatgt tgttctcagc
    38881 tacaatgcct aaagcaatcc aagctttagt acaacaattt atgaaatcac caaaaatcat
    38941 taagacaatg aataatgaaa tgtctgatcc acaaatcgaa gaattctata caattgttaa
    39001 agaattagag aaatttgata catttacaaa tttcctagat gttcatcaac ctgaattagc
    39061 aatcgtattc ggacgtacaa aacgtcgtgt tgatgaatta acaagtgctt tgatttctaa
    39121 aggatataaa gctgaaggtt tacatggtga tattacacaa gcgaaacgtt tagaagtatt
    39181 aaagaaattt aaaaatgacc aaattaatat tttagtcgct actgatgtag cagcaagagg
    39241 actagatatt tctggtgtga gtcatgttta taactttgat atacctcaag atactgaaag
    39301 ctatacacac cgtattggtc gtacgggtcg tgctggtaaa gaaggtatcg ctgtaacgtt
    39361 tgttaatcca atcgaaatgg attatatcag acaaattgaa gatgcaaacg gtagaaaaat
    39421 gagtgcactt cgtccaccac atcgtaaaga agtacttcaa gcacgtgaag atgacatcaa
    39481 agaaaaagtt gaaaactgga tgtctaaaga gtcagaatca cgcttgaaac gcatttctac
    39541 agagttgtta aatgaatata acgatgttga tttagttgct gcacttttac aagagttagt
    39601 agaagcaaac gatgaagttg aagttcaatt aacttttgaa aaaccattat ctcgcaaagg
    39661 ccgtaacggt aaaccaagtg gttctcgtaa cagaaatagt aagcgtggta atcctaaatt
    39721 tgacagtaag agtaaacgtt caaaaggata ctcaagtaag aagaaaagta caaaaaaatt
    39781 cgaccgtaaa gagaagagca gcggtggaag cagacctatg aaaggtcgca catttgctga
    39841 ccatcaaaaa taatttatag attaagagct taaagatgta atgtcttgag ctcttttttg
    39901 ttttcaataa ttgattctct gtagatatca aagtactaac gttttaaagg ttaaatattt
    39961 aattggattg agatctgtat gcggttatat cattctgtgt aaatatggtt ctccaccaaa
    40021 tgtggtgagt atataattta aagaactatt tttaaattaa gaataatcga acataaataa
    40081 actttatgaa atttcagtat catgttctta taaaaaacaa tagggctttt tgctgacgct
    40141 agtgcgcgat aaataataag ttgaatataa aaaagatcac tgccaatcat tcgtttaatg
    40201 gcagcgatct tttttattta attatttctc tttccactgc aacatttgat aaccaatgcg
    40261 tggatgtgtt ttaataatat cttttgcgtc ctcatgacat tgtgaaagtt tttgtcttaa
    40321 tgatgtcata tgcactctta aactaggcat ttcagtctta ttaacatagc catatattcg
    40381 ttttaatatc atctcataag tgagtacttt tcctttatga tttgacaata gttctaacaa
    40441 gctaaattca tttggcgtca aatgtacctc ctgattatta ataacaacag atttggagcc
    40501 aaagtcgatg cttagcaaac cgttagtaaa tacaatgtta gtttcttgat gtgacttagc
    40561 gattctctcg atgactcgta ttcgtgcccg aagctcatca acattaaaag gtttagtcat
    40621 atagtcattc gcaccgttat ctaaagcttg aataattgtt tgttcttctt gtcttgcact
    40681 tattacaatg ataggaatgt cagtatgttg cctgatttct gaaatcaaac ataatccatc
    40741 tttatctggt aaacctaaat ctaataaaat gacatctggt ttatcaattt gaattttaaa
    40801 gtgtgcttgt gtggcattgt cggctgtagt tacattgtaa taatctaaag ttaatgcaac
    40861 atcaagtaaa tgtgtgattg cgtgatcatc ttcaattatc aatattttag attgcattat
    40921 acgtctcctt cgttaaagtc tgtatatata ttgaaataga atatactgcc gtgtggttgg
    40981 ttcggtttat attgtaagtt tgattgatgt ttgtgtagga tagtctgtac taaatataag
    41041 cctagtccca tgctttcttt ttggttatct ttaaaatatt tatttgatcc tgtgtaaaaa
    41101 ggctcgaata tcttttgttg ttcttctaaa ctaattccag gtccttcgtc tataacggca
    41161 aattcgattt gttcatagct agcataacga atagataaat tgattttggt gtcagtagaa
    41221 gtgtgtttaa ctgcattttc aatcaaattg aataaagctt gtaaaatcaa cttactgtca
    41281 atgtgtataa actgtaaatt tactgaggat gatacagtta tacgcttttt taaatggcga
    41341 cgttctaaaa tcatatcgat ttcttctact aattcactaa cgagataagg ttgcaatttt
    41401 atctgaacat ttgatgactg taattttgtt aatgataaaa tatttgtcac taatagatat
    41461 aaatactgac tttcttgaaa actatgtaca agtaattgtt ccttttctat gatagacata
    41521 tctttactat gtgatactaa aatatctaaa tttcccataa ttgttgttaa cggtgtacgt
    41581 atgtcatgcg aaattgatct taaaaaattt gaatgtgtca gttgacgttc agcctgtaac
    41641 atggattctc tcgtttgttt aagtaacgtc acattttcaa cggcgagaga aagttcattt
    41701 aacattgatt ctaatattga tgcatcatat ggattaatca cttgagaact ttggtaatca
    41761 atggctagaa tgcctttaat cggagatgtg ccaattggta tcaaccattt attaatgcct
    41821 ggaaatgtat ctgttgttgc accagcttgt ctttcatttt taattaccca gcttaatgct
    41881 tgttcatgct gttgagtcgt attatcgata tggttttgca atggtattgt tttaattact
    41941 ttcgattgat tgataacgta tatagtaatt gattgttgca ataattgatt aatttggtat
    42001 ccagcattta ttagtaagtt ttcaactgta taagtttgtt taatcgaatc attaaattga
    42061 aataataaat ctgtacgata aagttgcttt ttagtaatgg agtattggaa tttaatttgt
    42121 tttaataaag cactcgttaa aatacttgtt aaaatgctaa cgataaatgt aataggatag
    42181 tcaaagcgat atacttcaaa tgtatatcta ggttccgtaa aaaaataatt aaaaacaaat
    42241 acgttaataa ttgctgctaa aaagccaatg atgaaggacc gcgtccaaat ggataataaa
    42301 atgatgccga tgagaaaaat cattaaaata atcgtactag actcatgctt atcaagttga
    42361 taaatccaca gtcccatcat tacacaaatt atttgaatca aaatcatttt taacatatct
    42421 atggcgaaac gtttgccttt aggacgatag ggtgatttgt tcattttcaa ttcaacaggt
    42481 atttgtttga ttggaacgat ttcgatttta tagctatgtt caaaggacat taaatggtca
    42541 attaaaggtg tattgaaaaa gtctcgccac ttatttctaa tatgttgtcc gataattaat
    42601 ttggttacat cttgattttt acaccattcg tctaatccta atgcaacggt ttggctataa
    42661 actactttta cttttgctcc taatgatttt gcaagcatga gatgttgatg cacttgcttt
    42721 tgactatctt tatattgcct gtttttttcg aatacatcta tataaatagc agtgaacttc
    42781 gcatgttctt tttgagcaat atggaatgcc tctttaatta ctgcttcatt ataaatgctc
    42841 ccactaattg ccacagcaat atgaggtttg agtgacgttt tatggttgtg tcggactttt
    42901 tctttatcac tcatcaagtc ggcaactgtt cttaacgtca atgtacgcag ttcgcttagg
    42961 tgggcatacg taaagaaatt actaaatgct acatctagcc tatccttttt atataccttg
    43021 ccagctttta agcgtttaat taattgttca ggtgagatat ctacgacttc taatacatcg
    43081 gcgctcatta tgaaatagtc gggtacacgt tctttaacat gtacaccggt catcagttca
    43141 atttgactac ttaaactttc aatatgttga atgttcaaag tggtatgaac atcgatacca
    43201 tgatttaaaa tttcttcaat atccatatat cgtttctcat gacgatctct agaaatattc
    43261 gtatgtgcta actcatcgat aagtactatt gtcggcgatt cttcgattat gcggtcgaca
    43321 tctaaatatt gaaaatgatg actaccatgt ttagtaaaat tggttgtgat tttaggtaat
    43381 tgttcagcta atgcatttgt ttcatcacgc tgatgcggtt caatatatcc tattttaata
    43441 tcaacgttac tttgaaatag ttcaatggca tttgaaagca tctcaaatgt tttaccaaca
    43501 cctggactgt aaccgatgta aattgttaaa gatccacgct tttttcctat gtttagcgat
    43561 tcagtgtttg acataacctt cacctcgata gcaatatcat aatattcttc acaattaaat
    43621 gctataaaat ttatttctgc atacacatct taatgatttc ttaataacta agtttgaaaa
    43681 ttaatatttc tctcataaac aagttgttac gatgttgttg tgttgtaagg aggttatggc
    43741 aatgctcatc gttttattac tggtagttat tgcattagtt ttgtatttat tttatgcctt
    43801 aattcgtagt gaaaaatttt agaaatatat taagtgaggg ttatcatgga aataattttg
    43861 tttctaacaa tgatggttat gattacgtat gtttttagtg gatatctata tcgcgtagca
    43921 ttggtgcaat cgtcaagagt agatttaata tttactagat ttgaaaatat gtgttttaaa
    43981 attatcggca cagatttaga acacatgtca gctaaaacat atgtcaaaca ttttctggca
    44041 tttaatggat ttatggggtt tataacattt gtattgctaa tagttcagca atggcttttt
    44101 ttaaatccaa atcataattt aaaccaatcg atagatttag cgtttaatac agcaatatct
    44161 tttttaacaa atagtaattt acaacactat aacggtgaat cagatgtgac atatttaacg
    44221 caaatgattg taatgacata tttaatgttt acatctagtg catcaggtta cgccgtttgt
    44281 atagcgatgt tgagacgttt aactggatta actaatatca ttggtaattt ttatcaagat
    44341 attgttcggt ttattgtccg agtactttta ccattatcat gtttaattag tattttattg
    44401 atgactcaag gtgtaccaca aacgttgcat gctaatttaa tgattcggac tttaagcgga
    44461 catattcaac atattgcatt tggacctatt gcatcacttg aatcaataaa acatcttggt
    44521 acgaatggtg gaggattttt agcaggaaat tctgcgacac cttttgaaaa tccaaatatt
    44581 tggagcaatt ttatagaaat gggcagtatg atgttacttc ctatgtcaat gttgttctta
    44641 tttggtcgca tgttaagtag acatggtaaa cgagtacatc gtcatgcgtt gatattattt
    44701 gtcgcaatgt ttttcatttt tatagcaatt cttacattaa ctatgtggag tgagtatcgt
    44761 ggtaatccaa tactagcgaa tttaggcatt tatggaccga atatggaagg taaagaggta
    44821 cggtttggag caggtttgtc agcactattt acagttatta cgacggcatt tacaacgggt
    44881 tctgttaata acatgcatga tagcttaacg cctataggtg gattaggacc aatggtatta
    44941 atgatgctaa atgttgtatt tggtggcgaa ggcgtaggac tcatgaattt attgatattt
    45001 gtcttactga cggtgtttat atgcagtttg atggttggta aaacaccaga atatttaaat
    45061 atgccaattg gcgcccgtga aatgaaatgt attgtcttag tctttctcat acacccaatt
    45121 ttaattttag tattttcagc acttgctttt atgattcctg gagcaagtga aagtataacg
    45181 aatccgtctt ttcatggtat ttcacaagtt atgtatgaaa tgacatcagc tgctgcgaac
    45241 aatggatcag ggtttgaagg actgaaagat gatacaacat tctggaatat ctctacagga
    45301 atcattatgt tgctttctcg ttatatacca attattttgc aattaatgat tgcatcaagt
    45361 ttagtgaata aaaaatcata ccatcaagat aaatatacaa tagcgattga taaaccttat
    45421 tttggcgtat cactaatcgt atttatcgtt ttactaagtg gtttgacatt tattccagta
    45481 ctattacttg gtcctattgg tgaattttta actttaaaat aaagtaaagg gatggaaaaa
    45541 atgcatcatg taaataaata tttcaatcaa acaatggtga tagaagcttt gaaaatgagt
    45601 ttttataagc tgaatcttaa acaattaata aaaaatccga taatgtttgt cgttgaggtt
    45661 ggaatgattt taacgttgat attaatatgt tttcctgaca tatttggaac tagctatcta
    45721 tcgcgaggtt atttgattac aatttttatc atattattaa taactatatt gtttgctaat
    45781 ttttcagaag cctttgctga aggtcgtgga aaagcgcaag ctgatagttt aagacaagca
    45841 caatcaaatt taactgctcg attgatagaa gaaaatggtg cgtatcggat agtgaacgcc
    45901 acagagttaa aagccggaca aaatattcgg gtagaaaatg gagaaacaat ccctgctgat
    45961 ggcgttgtaa taaatggatt agcaaccgtt gatgaatctg caattactgg tgagtctgca
    46021 cctgtgataa aagaatcagg tggcgatttt gatggagtca ttggcggtac acttgtaaca
    46081 tctgattggt tagaaatacg tgttgagagt gaagcgggta catctttttt agataaaatg
    46141 attgccttag ttgaaggggc ggaaagaaat aaaacaccaa atgaaattgc actttttaca
    46201 ttattaacaa cgttaacaat tatattttta gtcgttattg taacgttata tccaatagca
    46261 tcttacctgc atttaatttt acctattgcg atgctcattg cgttaacagt atgtttaata
    46321 ccaacgacaa tcggtggttt gttatcggcg attggtattg caggcatgga tcgtgttact
    46381 caattcaatg tattagcgaa aagtgggcgt gctgtagaag tatgtggaga tgtcgatgta
    46441 atgattttag acaagacagg cacaatcact tatggcaatc gaatcgctag tgaattttta
    46501 ccagtgaatc aacaaatgct tgaaaaattg atagtcgctg cttatatgtc atcaatctac
    46561 gatgatacgc cagaaggaaa aagtattgta cgattagcga agcagatgta tataaatgag
    46621 ctgcctaaag acattgatgg cacatataag ccttttacag ctgaaactag aatgagcgga
    46681 atcattacaa atgaaatatc ggtattcaaa ggtgcaccta atagtatgat aaatcttgtc
    46741 aaacaacaac aaggcaatat tcctcttaat attgagtctc tctgtatgga tgtatcgagt
    46801 aaaggtggca caccattaat agtcattgaa aacaatgtca tgcttggcgt gatttattta
    46861 aaagatgtga ttaaagatgg tcttgtagaa cgatttactg aattgagaaa aatggggatt
    46921 gaaacagtta tgtgtacagg tgataatgct ttaacagcag ctacaattgc aaaagaagcc
    46981 ggggttgata gatttgttgc agagtgtaaa cccgaagata aaattaaagt catcaaagat
    47041 gaacaagcga aaggccatat tgttgcgatg acaggagatg gtacaaatga tgcacctgcg
    47101 ctagcacaag cgaatatagg attagctatg aactcaggga caattagtgc taaagaggca
    47161 gctaatctaa ttgatttaga ttcgaatcct acaaagttaa tagaggttgt aaaaataggc
    47221 aaacaattat taatgacgag aggtgcatta acgacattta gtttggctaa tgacgtagca
    47281 aaatactttg caattttacc tgcactaatg atgtcaacaa ttccagaaat gacttcacta
    47341 aatattatgc atttatcgtc acctaaatct gcaattatat cagctttgat attcaatgca
    47401 ttaatcattg ttgccttaat tccaattgcc atgaaaggtg taaaagtaaa gggttacagc
    47461 attgatagaa tctttataaa taatatgttg atttatggat taggtggatt aatcgtcccg
    47521 tttttaggca ttaaattaat agatatgatt gtacaatttt ttgtgtaaag aggaaggagc
    47581 ctgtaccatg aatacgataa gaaatagtat ttgtttaacg ataataacta tggtattatg
    47641 tggattttta ttcccgttag ctatcacgtt aattggtcaa atattttttt atcaacaagc
    47701 aaatggcagt ttaattacgt atgacaatcg tatagttgga tcaaaattga ttggacagca
    47761 ctggacagag acacgatatt ttcacgggag accgagtgca gttgattata atatgaatcc
    47821 tgaaaaatta tataaaaacg gcgtatcttc aggtggaagt aatgaatcga atggaaacac
    47881 cgaattaata gcacgcatga aacatcatgt taaatttggg aattcaaatg taacaataga
    47941 tgctgcaaca agttctgggt cgggcttaga tccgcatatt acggtcgaaa atgcattgaa
    48001 gcaagcacct cgtattgctg atgctcgtca tgtatctact tcaagagtgg ccgacttgat
    48061 tcaacatcgt aagcagcgtg gtgtgttaac aaatgattat gtgaatgtgc ttgaactaaa
    48121 tatcgctctt gataagatga aagattgatt atagatgaaa ggcatcatgt tgataaaatt
    48181 atacatggtg tctttttagc aattcaagca ctttctgctt aattaaattt ttagatgtac
    48241 ctttaggaag aataaattag catgaa
//
LOCUS       DAKTLH010000006        44738 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.006, whole genome shotgun sequence.
ACCESSION   DAKTLH010000006 DAKTLH010000000
VERSION     DAKTLH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 44738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44738
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.006"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            86..1465
                     /gene="uhpT"
                     /locus_tag="O0394_000262"
     CDS             86..1465
                     /gene="uhpT"
                     /locus_tag="O0394_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCY0498378.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            complement(1824..2582)
                     /locus_tag="O0394_000263"
     CDS             complement(1824..2582)
                     /locus_tag="O0394_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0498379.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(2575..4131)
                     /locus_tag="O0394_000264"
     CDS             complement(2575..4131)
                     /locus_tag="O0394_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0498380.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKD
                     NRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            complement(4128..5096)
                     /locus_tag="O0394_000265"
     CDS             complement(4128..5096)
                     /locus_tag="O0394_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCY0498381.1"
                     /translation="MKSKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            5684..7933
                     /gene="pflB"
                     /locus_tag="O0394_000266"
     CDS             5684..7933
                     /gene="pflB"
                     /locus_tag="O0394_000266"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCY0498382.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            7956..8711
                     /gene="pflA"
                     /locus_tag="O0394_000267"
     CDS             7956..8711
                     /gene="pflA"
                     /locus_tag="O0394_000267"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCY0498383.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            9047..10810
                     /locus_tag="O0394_000268"
     CDS             9047..10810
                     /locus_tag="O0394_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HCY0498384.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWTEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSALFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDIVGLKMRQNGHEAKFVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHLENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(10974..11318)
                     /locus_tag="O0394_000269"
     CDS             complement(10974..11318)
                     /locus_tag="O0394_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HCY0498385.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAFQKRDILAAHYIAKSAIRTKNLDQMTKAKQRLESIY
                     NSISNPLHSQNN"
     gene            11508..13337
                     /gene="coa"
                     /locus_tag="O0394_000270"
     CDS             11508..13337
                     /gene="coa"
                     /locus_tag="O0394_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HCY0498386.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSGKSQVNAGSKN
                     GTLIDSRYLNSALYYLEDYIIYAIGLTNKYEYGDNIYKEAKDRLLEKVLREDQYLLER
                     KKSQYEDYKQWYANYKKENPRTDLKMANFHKYNLEELSMKEYNELQDALKRALDDFHR
                     EVKDIKDKNSDLKTFNAAEEDKATKEVYDLVSEIDTLVVSYYGDKDYGEHAKELRAKL
                     DLILGDTDNPHKITNERIKKEMIDDLNSIIDDFFMETKQNRPKSITKYNPTTHNYKTN
                     SDNKPNFDKLVEETKKAVKEADDSWKKKTVKKYGETETKSPVVKEEKKVEEPQAPKVD
                     NQQEVKTTAGKAEETTQPVAQPLVKIPQGTITGEIVKGPEYPTMENKTVQGEIVQGPD
                     FLTMEQSGPSLSNNYTNPPLTNPILEGLEGSSSKLEIKPQGTESTLKGTQGESSDIEV
                     KPQATETTEASQYGPRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETN
                     AYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHGNGQVSYGARPTQNKPSKTNAYNV
                     TTHANGQVSYGARPTYKKPSKTNAYNVTTHADGTATYGPRVTK"
     gene            complement(13519..13626)
                     /locus_tag="O0394_000271"
     CDS             complement(13519..13626)
                     /locus_tag="O0394_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498387.1"
                     /translation="MCVRTRLVSSSSARLSKAIIIAVIVVYHLDVRGLF"
     gene            complement(13940..15124)
                     /locus_tag="O0394_000272"
     CDS             complement(13940..15124)
                     /locus_tag="O0394_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCY0498388.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRAGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIQVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GFKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            complement(15154..17415)
                     /locus_tag="O0394_000273"
     CDS             complement(15154..17415)
                     /locus_tag="O0394_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HCY0498389.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKISIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDTIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPISHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYGAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            complement(17601..18812)
                     /locus_tag="O0394_000274"
     CDS             complement(17601..18812)
                     /locus_tag="O0394_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HCY0498390.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            complement(18924..20429)
                     /locus_tag="O0394_000275"
     CDS             complement(18924..20429)
                     /locus_tag="O0394_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HCY0498391.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFRAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDNDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILNQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            complement(20455..22032)
                     /locus_tag="O0394_000276"
     CDS             complement(20455..22032)
                     /locus_tag="O0394_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCY0498392.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPNDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQ
                     VKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEIYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
     gene            22473..23615
                     /locus_tag="O0394_000277"
     CDS             22473..23615
                     /locus_tag="O0394_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HCY0498393.1"
                     /translation="MSSASTQSTKTSDIHNESIDKQMEAKAHETAQNTDLKNEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMLSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVVLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            complement(23929..25404)
                     /locus_tag="O0394_000278"
     CDS             complement(23929..25404)
                     /locus_tag="O0394_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0498394.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKMLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(25602..25958)
                     /locus_tag="O0394_000279"
     CDS             complement(25602..25958)
                     /locus_tag="O0394_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCY0498395.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYAAK
                     DTKHNQAVVLQQLLNT"
     gene            complement(26115..26288)
                     /locus_tag="O0394_000280"
     CDS             complement(26115..26288)
                     /locus_tag="O0394_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498396.1"
                     /translation="MKLNQRYVKVFALYFVSIVTANIIVKNNNLIKTLIQTIAGYTVF
                     AVGLKYLTKRKNK"
     gene            complement(26314..27459)
                     /locus_tag="O0394_000281"
     CDS             complement(26314..27459)
                     /locus_tag="O0394_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HCY0498397.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGKNL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQV
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
     gene            27614..27721
                     /locus_tag="O0394_000282"
     CDS             27614..27721
                     /locus_tag="O0394_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498398.1"
                     /translation="MSVCTPTIERIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            28032..28985
                     /locus_tag="O0394_000283"
     CDS             28032..28985
                     /locus_tag="O0394_000283"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCY0498399.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYEEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(29305..30834)
                     /locus_tag="O0394_000284"
     CDS             complement(29305..30834)
                     /locus_tag="O0394_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0498400.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            31196..32131
                     /locus_tag="O0394_000285"
     CDS             31196..32131
                     /locus_tag="O0394_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCY0498401.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            32241..32339
                     /locus_tag="O0394_000286"
     CDS             32241..32339
                     /locus_tag="O0394_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498402.1"
                     /translation="MYVKNASKKHKTQKYMQKFQLLYFKRLKSQKI"
     gene            32470..34566
                     /locus_tag="O0394_000287"
     CDS             32470..34566
                     /locus_tag="O0394_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCY0498403.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLENHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQHEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDSISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKEVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRMHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            34551..35018
                     /locus_tag="O0394_000288"
     CDS             34551..35018
                     /locus_tag="O0394_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0498404.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            35041..35319
                     /locus_tag="O0394_000289"
     CDS             35041..35319
                     /locus_tag="O0394_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCY0498405.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            35546..36805
                     /locus_tag="O0394_000290"
     CDS             35546..36805
                     /locus_tag="O0394_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCY0498406.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQIICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQKVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            36823..37878
                     /locus_tag="O0394_000291"
     CDS             36823..37878
                     /locus_tag="O0394_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCY0498407.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAYHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKVLLKPLDLDENEGEN"
     gene            37880..38026
                     /locus_tag="O0394_000292"
     CDS             37880..38026
                     /locus_tag="O0394_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498408.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            38050..39093
                     /locus_tag="O0394_000293"
     CDS             38050..39093
                     /locus_tag="O0394_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCY0498409.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIIKVRATGICGSDTSR
                     YKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            39621..40337
                     /locus_tag="O0394_000294"
     CDS             39621..40337
                     /locus_tag="O0394_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCY0498410.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNVNGIN
                     NDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSEQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            40330..41355
                     /locus_tag="O0394_000295"
     CDS             40330..41355
                     /locus_tag="O0394_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCY0498411.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEQHHIIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKTYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            41377..43071
                     /locus_tag="O0394_000296"
     CDS             41377..43071
                     /locus_tag="O0394_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0498412.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKENITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     FESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            43499..44668
                     /locus_tag="O0394_000297"
     CDS             43499..44668
                     /locus_tag="O0394_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol--glycerophosphate
                     glycerophosphotransferase"
                     /protein_id="HCY0498413.1"
                     /translation="MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQNAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGQASQRICQTIFK"
BASE COUNT        14948 a         7226 c         8208 g        14356 t
ORIGIN      
        1 tggataattt aataatttta aggatattaa gcgcttacac cgacgtgata tatttggctt
       61 aacgaaaatg attgaggtga cagagatgaa cttttttgat atccataaga ttccgaacaa
      121 aggcattcca ttatcggtac aacgtaaatt atggcttaga aacttcatgc aagctttctt
      181 cgtagtgttc tttgtttata tggctatgta tttaattcga aacaacttta aggcggcaca
      241 accgttttta aaagaggaaa ttggattatc tacattagaa cttggttata tcggattagc
      301 atttagtatc acgtacggtt taggaaaaac attacttgga tattttgtcg atggacgtaa
      361 cacaaaacgt attatctcgt tcttacttat cttatctgcg attacagttt taattatggg
      421 atttgtttta agttactttg gttctgtaat gggattatta attgtacttt ggggacttaa
      481 cggggtgttc caatcagttg gtggacctgc aagttattca acgatttcaa gatgggcgcc
      541 aagaacgaaa cgtggccgat acttaggatt ctggaataca tcacataata tcggtggtgc
      601 catagcaggt ggtgttgcac tttggggtgc taatgtattc ttccatggaa atgttatagg
      661 gatgttcatt ttcccatcgg tgattgcatt acttattggt atcgcaacat tatttatcgg
      721 aaaagatgat ccggaagaat taggatggaa tcgtgctgaa gaaatttggg aagagccggt
      781 cgataaagaa aatattgatt ctcaaggtat gacgaaatgg gagatcttta aaaaatatat
      841 cctgggaaat cctgttatat ggattctatg tgtttcaaac gtctttgtat acattgtacg
      901 aatcggtatt gataactggg caccgttata tgtgtcagag catttacact ttagtaaagg
      961 cgatgcagtt aatacgatat tctactttga aattggtgca ttagttgcaa gtttattatg
     1021 gggctacgta tcagacttat taaaaggtcg tcgtgcaatt gtagctattg gctgtatgtt
     1081 tatgattaca tttgttgtct tattctacac aaatgctaca agtgtcatga tggttaacat
     1141 ttcattgttt gcattaggtg cgttaatctt tggtccgcaa ttattaattg gtgtatcatt
     1201 gactggtttt gttcctaaaa atgccatcag tgtagcaaac ggaatgacag gttcattcgc
     1261 gtatctattc ggtgactcaa tggcgaaagt tggtttggcg gctattgctg atccaacacg
     1321 taacggttta aacatctttg gatatacatt aagtggatgg acagatgttt tcatcgtctt
     1381 ctatgttgca ttattcctag gcatgattct attaggaatc gttgctttct atgaagaaaa
     1441 gaaaattaga agtttaaaaa tttaatataa atcggattaa aagtatcgcc aatctattgc
     1501 aatatagttg gcaatcctgc cccgacggca tgtgcgtgaa gagatgaaag atactgcttc
     1561 tacccttgca aatatatcat ctctatgtct cggggcagat cataattccc tgttatgaag
     1621 tatccttatt tgcccgactt agggtgactc aatgaattta ctccttacaa taaagacata
     1681 tagcggtgtc aatattgtag ggagtattgt tttatattta aactctctaa aaagcggact
     1741 gaaagaaaag tgaaaacttc tctatcagtc cgctttttca tagaacaaaa tggaggcgcc
     1801 ataatcatta gttatgtgct aatctatttt gcttgcttac aataatcact tggcgacatt
     1861 tgtaaatatt ttttaaaatg atagctaaac attttatact ctgaaaagcc tactttgtct
     1921 gcaatttcat agtgtttgta atgtcgatct aacaattgca gagattgtaa aatacgatag
     1981 cgatttaaat aatcgacaat tgtaatacca acatgatctt taaatgttcg catcgcatac
     2041 gattcactaa catcgatatg ttgaattaaa tctgaaacag tcactttcgt ttgataagat
     2101 tgcttaattt gatccacaat ctggtttaca taataatcat cgtattctac ttttaatagt
     2161 ggttggaagg catcatgaca agatgctaag ctacggccgt tctgtgattg ttgctctaat
     2221 aaggtacgga caagtcttcc taaaataact tctaattgtg catggtctac tggttttaat
     2281 aaataatcaa gaacatgatg ttgaatgccg gctttcatat attcaaagtc atcgtaactc
     2341 gataatatga tgacattaca atctagatgc gcaatatcat tgagtaaatc gacgccattt
     2401 ttacgtggca tacgaatatc agtaattact aattctggct gatgttgttg aattagtgat
     2461 aatgcttcaa caccatcttt agcagtgtat attgtattga aatgatagtc tccccaagga
     2521 atgatttgct ttaatccttc tcgaataatt cgttcatcat cacaaataac taccttaaac
     2581 atctacattc ccccttgaaa gtggtatttt ataacaaatt aacgtacctt gattacgctt
     2641 tgaaaaaata tggagtcgtg catgtgaacc atattgaatc attgctttat tgtgtaaatg
     2701 atttaatccc aaatgcttag tatcaaatac atcattatta agagattggc gtacatattg
     2761 caggcgagat gacgacatcc cgataccatt gtcgcaaact aaaacatgta aattctgacg
     2821 tgccaatgtc aggcgtatag taatgtccaa tgactcagta tctctaccat gtttaatagc
     2881 attttctatg agtggctgaa gcatcatttt accaattgtc tggtgacgcg cttcttcaga
     2941 actttcaata tggagcttaa tcatgtcatc aaaacggatg ttttgtattg caacatactg
     3001 ttcaatgtag ttcaactctt cgtttaattc cactgtatgt gagtttgtac gtaatgagta
     3061 acgtaacatt tgcgataatt gttggaccac agtttgtgct aatttcggag ataacgtaat
     3121 taaatattgt attgtttgca tcgtattgaa taggaaatga ggctggaatt ggcgttctat
     3181 ttcctttaac tgaatatcac gcaagcgacg ttctgtatgc tcgatagaat ggatcagttg
     3241 ctcatttgat tcaaataaat cgtaaatata attattaatt tcttctagtt cactgttgtt
     3301 ttttaaaggc gtatatgtac ctagatgacg atttttggca tagtaaattt tttgaataat
     3361 cgtttcgata tcttttgttt gtcgtttagc catattatct gcgctaatga aaccaaatat
     3421 tactagtaaa acaagaacta cggccataac aattaacaac gtgataccat cttcaatgtt
     3481 ttcatgtata tctttataaa taatgagacg atggtcagca tggtttaatt ttacagattc
     3541 attcataaat ccgaattgtt gtggtctata cttttcacct atagtaaaac ggtcatcgtt
     3601 ggcgtataaa atattgtcat attgatcaac gataagtgcg aattgtcggt tatctttctt
     3661 aatttcactt aaacgtgggg tgttagccat ataaatttta agcatatatg tactattttt
     3721 gaatttaagc tgatgcgttg aaaataaata catattttta gtgtttaaat gttcataatt
     3781 attggttata aactgatttg gtccagataa ttcataataa agtgttgcgg gctgttggtg
     3841 tattaatttt aataattcac gttttgtagc ggtcacatca tgatgatttg ttaaatcgag
     3901 ctcttgaaac gaattattat gctgtgtaat aaatgtctga atctgctttt cagtatgatg
     3961 taaagatgac tgactttcat caacatgttg atgaatcgta cgatgctcaa tccaaatata
     4021 gatggcatag aagcttacta gtccaataat aatgactaaa aatactggaa aaatagtaga
     4081 cgcaaataac gatcgtctta attgatgtct ataaggtttg tatgccgtca ttgaatcatc
     4141 tccaaaaatt tatgatgtgg aatatccggt aatttagatt tcggtattaa aggtatgttc
     4201 ttaagatttt cgatagactg atcgctttgt tcactaacat cctttcgaat tgacttggca
     4261 tcgaactctg caactaatcg ttgttgtact gagcggcttg ttaaatattg cactaacttt
     4321 ttacgcttag gatgagggtg tgcattttta actaaagcaa taccatcaac atttaacatt
     4381 gttccttcaa ttggataaac gattgataca ggataacctt tgtttttcca tgtgcgtgca
     4441 tcttgttcgt agcttagacc tgcgtaatat ttaccttttg caacatcttc aatgacttta
     4501 gacgtctttg acagttgcat cgcatggttt tggaattgat gcacatcact tactcgatga
     4561 tgcatgctat aaatagcacg catatgttga tagcctgtcg ttgttgtatt tggatttgag
     4621 tacgcaattt tacctttaag tataggttgt aataaatctt gataacctcg aatcttaata
     4681 tctccttgta aatctgaatt cactactata actgttggca ttaatagaaa actagtaaca
     4741 tatttattgt tcgagcgata atcctctaat tgctgtgtta cagatgtatc ttgataggga
     4801 acaaaatctt ctggatgatc aattgtttct gacaacacac cacccataaa gacatcacca
     4861 cgctccgaaa aatcttcgtt atgcaagttt gaaagcagta cttgagtaga tccgtgttta
     4921 atttcaattt tgacatgctc ttgtttttca aattcattta aaattggacg aatcaagttt
     4981 gattgatacg gagaataaac tgttaataca tttttatcgg attcagagtg acgcgtatta
     5041 gcgcatgctg ataaaaaaat gagaaataat agcaagatat aaatttttga tttcatgata
     5101 tcccatcaat tctatgtata ttttaataca ataattttag caataaatga cgcataagta
     5161 atgttaaata tttagaaatg tttatagatg acttgttaag acgttgcaaa tgttgtgata
     5221 gcacaaaatt tttgtttgtc aagacgattt accgaggctg taaaatcaaa ctgttatatt
     5281 ttatttgtag ctgttatata aaaatcggca agatattgaa cggttcaaaa gtgaattttt
     5341 acgtcaataa aagtatttaa tccagtctct tcatatataa aagtaaatct ttctaagtgt
     5401 tgatttaacg cttatcaaca atcatttttt ataaacaaat atatactcct aaattaactt
     5461 ttaaagcaat gaaaatagtg aacattataa ctgttgtgta acagaatgca attagcatat
     5521 tactgttaca caaattagta cagtttctat gttttgacat acatttgatg aaaattgtac
     5581 ataatttatg tgaaaaaaat cacaacaaac atgctacaat gactatgaaa acgttaacat
     5641 agcatttcaa attcacaaca ttatacagat ggaggcgttt agtatgttag aaacaaataa
     5701 aaatcatgca acagcttggc aaggatttaa aaatggaaga tggaacagac acgtagatgt
     5761 aagagagttt atccaattaa actacactct ttatgaaggt aatgattcat ttttagcagg
     5821 accaacagaa gcaacttcta aactttggga acaagtaatg cagttatcga aagaagaacg
     5881 tgaacgtggc ggcatgtggg atatggacac gaaagtagct tcaacaatca catctcatga
     5941 tgctggttat ttagacaaag atttagaaac aattgtaggt gtacaaactg aaaagccatt
     6001 caaacgttca atgcaaccat tcggtggtat tcgtatggcg aaagcagctt gtgaagctta
     6061 cggttacgaa ttagacgaag aaactgaaaa aatctttaca gattatcgta aaacacataa
     6121 ccaaggtgta ttcgatgcat attctagaga aatgttgaac tgccgtaaag caggtgtaat
     6181 cactggttta cctgatgcat acggacgtgg acgtattatc ggtgactatc gtcgtgtagc
     6241 tttatatggt gtagatttct taatggaaga aaaaatgcac gacttcaaca cgatgtctac
     6301 agaaatgtca gaagatgtaa ttcgtttacg tgaagaatta tcagaacaat atcgtgcatt
     6361 aaaagaatta aaagaacttg gacaaaaata tggtttcgat ttaagccgtc cagcagaaaa
     6421 cttcaaagaa gcagttcaat ggttatactt agcatacctt gctgcaatta aagaacaaaa
     6481 cggtgcagca atgagtttag gtcgtacatc aacattctta gatatctatg ctgaacgtga
     6541 ccttaaagca ggcgttatta ctgaaagcga agttcaagaa attattgacc acttcatcat
     6601 gaaattacgt attgttaaat ttgctcgtac acctgattac aatgaattat tctctggaga
     6661 cccaacttgg gtaactgaat ctatcggtgg tgtaggtatt gacggacgtc cacttgttac
     6721 gaaaaactca ttccgtttct tacactcatt agataactta ggtccagctc cagaaccaaa
     6781 cttaacagta ttatggtcag tacgtttacc tgacaacttc aaaacatact gtgcaaaaat
     6841 gagtattaaa acaagttcta tccaatatga aaatgatgac attatgcgtg aaagctatgg
     6901 cgatgactat ggtatcgcat gttgtgtatc agcgatgaca attggtaaac aaatgcaatt
     6961 cttcggtgca cgtgcgaact tagctaaaac attactttac gctatcaatg gtggtaaaga
     7021 tgaaaaatct ggtgcacaag ttggtccaaa cttcgaaggt attaacagcg aagtattaga
     7081 atatgacgaa gtattcaaga aatttgatca aatgatggat tggctagcag gtgtttacat
     7141 taactcatta aatgttattc actacatgca cgataaatac agctatgaac gtattgaaat
     7201 ggcattacat gatacagaaa ttgtacgtac aatggcaaca ggtatcgctg gtttatcagt
     7261 agcagctgac tcattatctg caattaaata tgcacaagtt aaaccaattc gtaacgaaga
     7321 aggtcttgta gtagactttg aaatcgaagg cgacttccct aaatacggta acaatgacga
     7381 ccgtgtagat gatattgcag ttgatttagt agaacgcttc atgactaaat tacgtagtca
     7441 taaaacatat cgtgattcag aacatacaat gagtgtatta acaattactt caaacgttgt
     7501 atacggtaag aaaactggta acacaccaga cggacgtaaa gctggcgaac catttgctcc
     7561 aggtgcaaac ccaatgcatg gccgtgacca aaaaggtgca ttatcttcat taagttctgt
     7621 agctaagatc ccttacgatt gctgtaaaga tggtatttca aatacattca gtatcgtacc
     7681 aaaatcatta ggtaaagaac cagaagatca aaaccgtaac ttaactagta tgttagatgg
     7741 ttacgcaatg caatgtggtc accacttaaa tattaacgta tttaaccgtg aaacattaat
     7801 agatgcaatg gaacatccag aagaatatcc acagttaaca atccgtgtat ctggttacgc
     7861 tgttaacttc attaaattaa cacgtgaaca acaattagat gtaatttctc gtacattcca
     7921 tgaaagtatg taacaaaatt taaggtggga gcactatgct taagggacac ttacattctg
     7981 tcgaaagttt aggtactgtc gatggaccgg gattaagata tatattattt acacaaggat
     8041 gcttacttag atgcttgtat tgccacaatc cagatacttg gaaaattagt gagccatcaa
     8101 gagaagtcac agttgatgaa atggtgaatg aaatattacc atacaaacca tactttgatg
     8161 catcgggtgg cggtgtaaca gtcagtggtg gcgaaccatt gttacaaatg ccattcttag
     8221 aaaaattatt tgcagaatta aaagaaaatg gtgtgcacac ttgcttagac acatcggctg
     8281 gatgtgctaa tgatacaaaa gcatttcaaa ggcattttga agaattacaa aaacatacag
     8341 acttgatatt attagatata aaacatattg ataatgacaa acatattaga ttgacaggaa
     8401 agcctaatac acacatcctt aacttcgcgc gcaaactgtc agatatgaaa caacctgtat
     8461 ggattcgaca tgtccttgtg cctggttatt ctgatgataa agacgattta attaaactag
     8521 gggaatttat taattctctt gataacgtcg aaaagtttga aattctgcca tatcatcagt
     8581 taggtgttca taagtggaaa acattgggca ttgcatatga attagaagat gtcgaagcgc
     8641 ccgatgatga agctgttaaa gcagcctacc gttatgttaa cttcaaaggg aaaattcccg
     8701 ttgaattata aatacaattc agaccgaaaa gaaagcatat gcaacttcaa gagtgaaggg
     8761 gcatatgctt ctttttcaat tgagtattga gtattagcaa gacgtagtaa gtatatgaga
     8821 caacttctac aatggttgaa ggaagacgtt tttgtaagta gctatgctga taaagaatgt
     8881 gatgtcttgt taaaggtggg gttccaatat catcatttag ctgatgttga atgggttatt
     8941 atttgctact tgcatatgaa tatgagtctt ttcaaatttt tattgaccct gagtaatgaa
     9001 aaatattaag atgaaactta atattaaagc aatgcggagc gtgattatga agagaattag
     9061 taaagatata tgggcagtat ttaaattact gtatcaaaat aaagggcgtt ttagcattaa
     9121 tgccttacta ttgcagttaa tcatgatttt tattagtagt acatacttaa ttttactatt
     9181 taatatgatg ttaaaagtag ctgggcaaag ccaacttacg attaacaatt ggacggaaat
     9241 cgttagtcat cccgccagtg tgatacttct tattatattc atattaagtg ttgcctttct
     9301 gatttatgta gagttttcat tgttagttta tatggtttat gccggctttg atcgacagat
     9361 tattacattt aaatccattt ttaaaaatgc ctttgtaaat gtgcgtaaac tcataggtgt
     9421 accagttatt ttctttgtca tttatttaat gttaatgata cccattgcca acctaggact
     9481 aagttcagta ttaacaaaaa atatttacat acctaaattt ttaacggaag aacttatgaa
     9541 aacgacgaaa ggtataatca tttacggtac ctttatgatt gctgtattta tattaaattt
     9601 taaattaata tttactctac cgttaacgat tttaaaccgc cagtcgttat ttaaaaatat
     9661 gagactaagt tggcaaatta cgaagcgaaa taagtttcgg cttgttatag aaatagttat
     9721 attagaactc atcattggtg cgattttaac attaattatt tcaggagcaa catatcttgc
     9781 tatttgtgta gatgaagaag gagataagtt tttagtctca tcaattttat ttgttgtatt
     9841 gaaaagcgca ttgttcttct attatttatt tacgaaatta tcattaatca gtgtgttagt
     9901 actgcactta aaacaagaga atgtattaga ccaaccgggc ttagaattta aatatccaaa
     9961 accgaaacgg aagtctaggt tctttataat ttcaatggtg cttgcagtga catgttttat
    10021 cggttataac atgtacttac tttacaataa tactatcaat acaaatatct ccattattgg
    10081 ccatcgtggt ttcgaagata aaggcgttga aaattctatt ccgtcattga aagctgctgc
    10141 aaaagcgaat gtcgaatacg ttgagttaga tacaattatg acgaaagata aacaatttgt
    10201 tgttagtcat gataacaatt tgaaacgttt aacaggtgtt aataaaaata tttctgaatc
    10261 taatttcaaa gatatcgtcg gtttgaaaat gcgtcaaaat ggacatgaag caaaatttgt
    10321 atccttagac gaatttattg aaacggctaa acaatcaaat gtgaagctac tagtagagtt
    10381 aaagccacat ggtaaagaac cagcagatta tacacaacgt gttattgata ttttgaaaaa
    10441 gcatggtgtt gaacatcaat atcgtgtgat gtctttggat tatgatgtga tgactaagtt
    10501 gaaaaaagaa gcgccatatc tcaagtgtgg ttatatcatt ccgttgcagt ttggtcattt
    10561 taaagaaaca tcattagatt tctttgtcat cgaagatttt tcttattcgc caagacttgt
    10621 taatcaagcg cacttggaaa ataaagaagt ctatacttgg actattaacg gcgaagaaga
    10681 tttaacgaaa tacttacaaa ccaatgttga tggtattatc acagatgacc cagcattagc
    10741 tgatcagatt aaagaagaaa agaaagacga aacatacttc gatcgttcta taagaatttt
    10801 gtttgaataa tataaacaaa gacctctaaa gttatcaaga tgataccttc agaggtcttt
    10861 ttaatgttgc catctatggg ataggcaatc gtttcattcg tttatattca tatgacaagt
    10921 atttgtatgg caatttggcg tcacaaacac ttacatgatt tattggtgaa ttattaattg
    10981 ttttgtgaat gcaaagggtt agaaattgaa ttgtaaatac tttctaatct ttgtttcgct
    11041 ttagtcattt gatccaaatt tttagtgcgt atagcggatt ttgcaatata gtgcgcagct
    11101 aaaatatcgc gtttttgaaa cgcatctaaa tttaggtacg ataatttatt taagtcagtg
    11161 tttgctatta attcatgtaa ttgatctaca agcgcttgat gttgatacgt atgtgatgta
    11221 gtttcagatt tgcttgctaa tttaatacca gtcgtatcaa ggagcgccgc tttaatacca
    11281 gcaactaaat atgttttgat tttcatttgt gttgtcatgc tttgttactc ctttgatgta
    11341 cattaatcaa aaaaattata cactattgta tattgcaaag ctaattaact ataacaaaaa
    11401 gatagttaat gctttgttta ttctagttaa tatatagtta atgtctttta atattttgtt
    11461 tctttaatgt agattgggca attacatttt ggaggaatta aaaaattatg aaaaagcaaa
    11521 taatttcgct aggcgcatta gcagttgcat ctagcttatt tacatgggat aacaaagcag
    11581 atgcgatagt aacaaaggat tatagtggga aatcacaagt taatgctggg agtaaaaatg
    11641 ggacattaat agatagcaga tatttaaatt cagctctata ttatttggaa gactatataa
    11701 tttatgctat aggattaact aataaatatg aatatggaga taatatttat aaagaagcta
    11761 aagataggtt gttggaaaag gtattaaggg aagatcaata tcttttggag agaaagaaat
    11821 ctcaatatga agattataaa caatggtatg caaattataa aaaagaaaat cctcgtacag
    11881 atttaaaaat ggctaatttt cataaatata atttagaaga actttcgatg aaagaataca
    11941 atgaactaca ggatgcatta aagagagcac tggatgattt tcacagagaa gttaaagata
    12001 ttaaggataa gaattcagac ttgaaaactt ttaatgcagc agaagaagat aaagcaacta
    12061 aggaagtata cgatctcgta tctgaaattg atacattagt tgtatcatat tatggtgata
    12121 aggattatgg ggagcacgcg aaagagttac gagcaaaact ggacttaatc cttggagata
    12181 cagacaatcc acataaaatt acaaatgaac gtattaaaaa agaaatgatt gatgacttaa
    12241 attcaattat tgatgatttc tttatggaaa ctaaacaaaa tagaccgaaa tctataacga
    12301 aatataatcc tacaacacat aactataaaa caaatagtga taataaacct aattttgata
    12361 aattagttga agaaacgaaa aaagcagtta aagaagcaga tgattcttgg aaaaagaaaa
    12421 ctgtcaaaaa atacggagaa actgaaacaa aatcgccagt agtaaaagaa gagaagaaag
    12481 ttgaagaacc tcaagcacct aaagttgata accaacaaga ggttaaaact acggctggta
    12541 aagctgaaga aacaacacaa ccagttgcac aaccattagt taaaattcca cagggcacaa
    12601 ttacaggtga aattgtaaaa ggtccggaat atccaacgat ggaaaataaa acggtacaag
    12661 gtgaaatcgt tcaaggtccc gattttctaa caatggaaca aagcggccca tcattaagca
    12721 ataattatac aaacccaccg ttaacgaacc ctattttaga aggtcttgaa ggtagctcat
    12781 ctaaacttga aataaaacca caaggtactg aatcaacgtt aaaaggtact caaggagaat
    12841 caagtgatat tgaagttaaa cctcaagcaa ctgaaacaac agaagcttct caatatggtc
    12901 cgagaccgca atttaacaaa acacctaaat atgttaaata tagagatgct ggtacaggta
    12961 tccgtgaata caacgatgga acatttggat atgaagcgag accaagattc aataagccat
    13021 cagaaacaaa tgcatataac gtaacaacac atgcaaatgg tcaagtatca tacggagctc
    13081 gtccgacaca aaacaagcca agcaaaacaa acgcatataa cgtaacaaca catggaaacg
    13141 gccaagtatc atatggcgct cgcccaacac aaaacaagcc aagcaaaaca aatgcataca
    13201 acgtaacaac acatgcaaac ggtcaagtgt catacggagc tcgcccgaca tacaagaagc
    13261 caagtaaaac aaatgcatac aatgtaacaa cacatgcaga tggtactgcg acatatgggc
    13321 ctagagtaac aaaataagtt tgtaactcta tccaaagaca tacagtcaat acaaaacatt
    13381 acgtatcttt acaacagtaa tcatgcattc tatgatgctt ctaactgaat taaagcatcg
    13441 aacaatcgga agcatatttc taaattattt attcattata gtcttaaaca taacatgacc
    13501 taatatatta ctaacctatt aaaataaacc acgcacatct aagtgatata cgacaatcac
    13561 agcaataata attgctttag aaagtcgtgc cgaactggaa cttacaagtc tagttcgaac
    13621 acacactgat gtgagtggtt ttctttattt taaacatgaa caatcagata agttactagc
    13681 attagcaaat attattaaat caaagggctt cgattcataa aatttaaaac aatgattaaa
    13741 attagacgtg taaatgttaa attctaaaac ggaaataacc accatcccat taaaccactt
    13801 ttttgttcaa tcactatatt tcacacagct tcattaataa aacgaaattg cttcaacccg
    13861 cttcaacttc aactggcttc aacttcagcc tacttcattc aataacaaaa cgaatccgct
    13921 tcatccaaaa tcaaccattc taacgcacat attcaaatat agcagctgca cccatgccga
    13981 caccaataca catcgtaacc atgccgtaac ggctatcggg acgtctaccc atttcattaa
    14041 gtaaacgcgc ggttaacatt gcgcctgtag cacctaatgg atgacctaaa gcaatagcgc
    14101 caccattcac attcgtacgt gatatatcta gacctacttc tttaatagat gcaatcgttt
    14161 gagaagcaaa tgcttcgttc aattcgatca aatcaatgtc ttcaacagat agattgctga
    14221 gtgacaatac ttcaggaatc gcatatgcag gcccaatacc cataattttc gggtcaacgc
    14281 ctactgcctt aaaaccaacg aatcgtgcaa taggtgtcac gccgagttct ttcactttat
    14341 ctccagacat taaaactaca aatcctgcac catcagaaag tggggcagat gttcctgcag
    14401 tcacagtgcc gtcagcttta aatactgtac gtaatttggc taatgcctcc atcgtggtgt
    14461 cagggcgtat aaattcatct tggtcaaaga tatttgtgtg tacttttggt cctgcgtttg
    14521 tatattcaac tgagtttact tgtattggaa taatttcatc tttgaaccga ccatcacgtt
    14581 gtgcgtcata ggcacgttga tgacttctga cagcataagc atcttgatct tcacgtgata
    14641 cgtcaaattg ggatgctaca ttttcagcag ttaaacccat aggatatgac gcacctatat
    14701 catcatattg taaggttgga ttgtttgtgg gctcgttgcc acccattggt acggcactca
    14761 tcaattcaac gccaccagct acaagtatat ctccttgacc agccataatt tgattggctg
    14821 caatcgcgat ggtttgtaat cctgatgagc agtagcgatt cactgtttga cccggtaccg
    14881 tgtcagataa tcccgcacgc aatgcaatcg ttcgtgcaat gttttggcct tgtaatcctt
    14941 ctggaaaagc cgtaccaaca atgacatctt caatcatatt cttattgaat tttccgtcaa
    15001 tacgtttcaa tacgccttgt aatactttgg ctgcgacatc atcaggtctt tcgtggaata
    15061 atgcgccttg ctttgctttc gctgcggctg aacgcccata agctacaatg tatgcttctt
    15121 gcatggttat catcctctct taatgactat cttttaatta cgtaatggct taccagtttt
    15181 taacatatgt gcaattcttt catatgattt tttagatttt agtaagtcaa taaagccaat
    15241 tttctccaac gattgaatgt aacgttgatt gataaatgta tttcttggta aatcaccacc
    15301 cgctaaaatt gtggcgatat ttaaggcaat atgataatca tggtcgctaa taaaatgacc
    15361 ccgtctttgc gcatctaatt gtccttggat caatgctttg aagtcttcac ctaaagcgat
    15421 atattgatgt ctaggattcg gaatatagtt tgtttctgct ccatatttcg cacgtttgag
    15481 cgcaacttcg acacgttgtg ctgtattgaa aataatcgta tctgtatcac gtaaataacc
    15541 ataacgacgt gcctcaaagg catttgtaga gactttcgca aatgcgatat tcgtcagtac
    15601 ttttgtcatg gaagcttgtt tgtcatcaaa cttatgcgat gtgcgtaata tgcgatcagc
    15661 catttctgca aggccaccgc cactcggtaa taagccaaca cctgcttcaa caagaccgat
    15721 atatgtttca cttgcagcga caacaatagg tgagtaaagt acaagctcac agccaccgcc
    15781 taaggcacga ccttgaacag ctgtgactac tggtttcaaa ctatacttca aacgattaaa
    15841 gctataatgt aatttatcaa ttgattgtgc aacgacatca tctacaagac cgtcttcatg
    15901 cgcctttttc attaagaaaa ggttagcacc cacactgaaa ttgttaccat ctgcataaat
    15961 aaccatactt gtgtaatggt cattttccag taaatcaatc gcatcaacta acgcatcgtt
    16021 gaattcatcg gtaatgacat tatttttact ttgtaatttc agtaacagtt gatcatcatg
    16081 agttacggaa agtttggcat cacctttatc ccaaagttca tcttttacga agtgagaaat
    16141 aggtgttgca tattcaatgg tctcatcttg tttataaaag ccaccatcta aatcactaat
    16201 ccattgtggt aagtctccaa gttcgtcttc catacgtgtt ttaacacgtt cgtatcccat
    16261 tgcatcccat aattggaatg gaccaagttt ccagttgaac ccccagacaa gcgcacggtc
    16321 tatgtctcgg aaatcatcgg tagctttagg tacattgata gcagagtaat agaaattatt
    16381 acgtaatgtc tcccataaaa atagtcccgc ttcgtcttgc gcattgaata tggtatcaag
    16441 gttatgcact aagtctttat taaattcatt taaaattggt aattgtggtt gcgatacagg
    16501 tacataatct tgtttttcaa catcgtaaac aagtcgagct ttagtttctt tatccttttt
    16561 gtaaaatcct tgtttcgttt tacgtccgag tgcgccattg tcaaacaacg tatttacaat
    16621 tttgacatca tgaaaataag gtgtttcttc aggtacttgt tgcatgcctt taattacaga
    16681 cactgcaata tctaaaccga ctaggtcaga tagcgcatat gtacctgttt taggacgacc
    16741 aatcgcttgc ccagttaaag catccacatc tacaatgctt atcttgtgtt gctcggcgcg
    16801 atacataata tcattcattg tttgcgtgcc gactctattt gcgacaaagc caggcacatc
    16861 attgacgaca atgacacctt tacctaacac attttgcgcg aaatttttta catctaatat
    16921 aatagattcc ttcgtgtgtg acgtaggtat taactccact aatttcataa tacgtggtgg
    16981 gttaaagaaa tgtagaccaa agaatcgttc ttgatccttc tcgttaaatg cttgagcaat
    17041 cgcattaatt ggaatacctg atgtatttgt agcgaataaa gcatcttctt tagcatgttg
    17101 tagaacttgt tgccaaacag catgcttaat ttcaatatct tctttgactg cttcgatata
    17161 taaatcagca tcatcattta ccaagtcatc atcaaaatta ccatatgtta aatgactcgc
    17221 tagatttaag tcgaatagta gcggccgttt cttatctgta attttatcgt aagatttttt
    17281 cgcaatgaga tttggatcgt ttttgtccac tacaatatct aatagtttta ctttaagtcc
    17341 agcattcaca aaaagtgctg ccagttgagc gcccattgtg cctgcgccaa gaacggttac
    17401 tttattaatt gtcatagtga ttcctccaat ttagttgagg ataagataac cattaagata
    17461 attggaataa cgttgctatt ttataaaatt aattaagtat ctttgacagt catcttagcc
    17521 tcttatttaa ggaaaaagct ttatgcttaa aataagtctt ttttagtgaa attaatgcat
    17581 ctcatataat tatttgctat ttatacgaaa gcagaatctc cagtcaaagc gcgtccaatt
    17641 actaaggcat taatttcatg tgtaccttcg tacgtgtaaa tcgcttctgc atcagagaag
    17701 aaacgtgcaa tatcataatc gtcagctagt atgccattac cacctgtaat accgcggccc
    17761 atagctactg tctcacgcaa acgtaaggca ttcatcatct tcgccgttga agttgcaacc
    17821 tcgtcatatt caccatgtgc ttgcatatta gctaattgag cacatgttgc cattgcttga
    17881 gctaaattac cttgcatcat tgctagcttt tcttgtatta actgatattt actaattggt
    17941 ttgccgaatt gcttacgctc agtgacataa tctaatgtgg cacgtaaagc gccagccata
    18001 ccacctgtag ccatataagc aacgcctgct ctcgttgaat aaagaatttt ggcaatatct
    18061 ttaaagcttg ttatgttttg taagcgatcc gcttcatcta ctttgacatt agttaattta
    18121 attagggcgt taggaacaat gcgaagtgcg attttattat caatgacttc aatatcgacg
    18181 ccatcttgtt ctggtctgac tacaaagcaa tggggtttgc cagtttcttt atttactgcg
    18241 aatactggaa tgacatcaga tacatgtgca ccaccaatcc atttcttttc accattgata
    18301 acccaagtat cgccttggcg ttcagcgact gtttcaagac ctcccgcaac gtccgaaccg
    18361 tgttctggtt cagttaaagc aaagcatgta cgcagttcat gtgactgtaa tttaggtaca
    18421 tatttcgcaa tttgttcttt gctacctccg aaatagaaag tgttatgccc taaaccttgg
    18481 tgaacaccga gtagggtagc taaggaaata tcaaatcgcg cgagtaggta agacatgaaa
    18541 aactgaaata gttgactagg cattttggcg tttggacgat ccttgtaaag taatggattg
    18601 ttaaaataat ttaattctcc cagatcttta aaatagtcct cgggtacagt agcgtctatc
    18661 caatgttgat taatattttc acggtactta ctttctagca atgaatctac ttgttgtaaa
    18721 aattcgactt caccgtctgt taaaccttta gcaatactaa gtacatcttc aggaaataat
    18781 gtttttaaga ccgtttcttt ttcaaatgtc atataaattc ctcctaaaaa taatatgaat
    18841 actaatgtga aatgcattta attcaaaaac aacacgcttt atttgtaaac gcttacacta
    18901 aatgtcaaaa atttttatca cctttaaagt gtttgcgaga ctttgtcatt catcatttgt
    18961 cgaatcgcaa gtttatctgg tttctgcgta ctgtttaacg gcatatgtgt cactggtaca
    19021 tacattcttg ggactttata acctgctaaa cgacttcgca tatgttgatt taaaatttca
    19081 gcgtaatgag gttcatcttc gcgaagtata atggctgcag caattgattc accatatttt
    19141 ggatgatcat agccaacgac cacacaccgg tctactagtg gatgctcagc taaagcattt
    19201 tcgacttcgg atggtaagac attttcgcca ccagttatga ttaattcttt tttgcggtca
    19261 ataataaata tatcgccatc gttgtccatc ttcgctaagt caccagttaa taaatatcga
    19321 ccatgaaatg ctttggcagt ctctgctggt ttattccaat atcctggcgt gacattttta
    19381 gccttaattg caagttcgcc aatctcacca gtaggtactt cctcaccgtt atcatcaagg
    19441 atacgtgcat caacgaacat gactgcttta ccaatactca ttggcttacg ttttgaattt
    19501 tccggtgtat taacaagtac aagaggtgct tcagttaaac catagccgtt aataatgttt
    19561 atgccatatt gtttaaaagc tgcttggata cttggtaatg gttgtgaacc accttggatg
    19621 atataatcca tagctctaaa attttcagga ttaaaattac tagcacgtag cgtactataa
    19681 tacattgtcg gaatcatgat aataaatgta gggtgatatt gtgcaatcat gtcattcaat
    19741 tcttcgccgt taaagtaacg ttgaagaata agtgtgccac ctgacattaa tactggtaat
    19801 acagtatcgt taaaccctaa aacatggaac attggtgttg atacaatcgt aatatagttt
    19861 gaattgaact tatacgtcag ctctaagttt gcaccgttat gaacaaatga ttcatatgag
    19921 aacatcacac ctttaggtga tccggttgta ccacttgtat aaattaatgc tgcaagatct
    19981 tgtggttcaa caggtgttgc ttgaaaaggt tggtgataat ctggatttac gatttcatca
    20041 tattgcgcca catcaatatc catatgcaat aagttttggt caatatcggt gagtgaactt
    20101 aaatgttttt cagcatagaa gagcagtttt aattgtgcat cttccacaat ggctgcaatt
    20161 tcttttgggt taagccgcca attcaatggt aaaaaaaccg cacctgtttt aaaacaagca
    20221 aacaataaat ctaatattgc aatatcattt ggcgcaaaaa taccgataac atcgcctttt
    20281 ttaacacctt gagatgttaa ataatgtgcc atattatcag cgcgtgcatt gagttgttgg
    20341 tatgtccaag atgtttgttt tgcgtgatca ataacggcag gcttgtcatc atcgaagtct
    20401 gaacgcgttt ttatccaatc gaaattcatt agtatacccc ctttagcttc actttcatac
    20461 tttatgaatt gattgtttaa gttgtcccca tttttctttg taaatgctgg tatcaattaa
    20521 ttttaaatga tcagcaataa ttggtttaaa agccatttga ttcaaaatat ctttatgcaa
    20581 atcaagacct ggtgcaattt caattagttt caagccttga ttggtgagtt cgaatactgc
    20641 acgatcagta acaaaataga tttcttgctc gagtgattgt gaatattgtg cattaaagtc
    20701 gatatggctc acatctgata caaatttctg gttttgtcct tcagtttcaa tgtttaatcg
    20761 ttgattatgg catgagacat gactgccagc tacaaaagta cctgaaaaga taattttatt
    20821 tacagattgc gtaatgtcta taaagccacc acatccattt agtcggtcat tgaagtaaga
    20881 cacgttgaca ttgccgtatt gatcaacctc agcaaagcta agataggcaa ctgatacacc
    20941 attgttataa ataaaatccc atgctcgatc atgaggcatg cgcacatctg cattgtaatt
    21001 cataccaaaa tgttcacgac tcccaacgaa tccaccgaaa atgccaacat ctaaaatcgg
    21061 ttgcacatca tgttcaacac attcttcatg caataaatta gagagttcat tattgatgcc
    21121 ataaccgatg ctaattgtat cgccataagt taaaaactga gcagcacgtc ggagaatcaa
    21181 tttgcgacta ttaaaaggta atgcgggttc aggtattcca tcaattcgtt cttctccaga
    21241 caaggctggt aaataatgac tttgaattac ttggcggtga ttcttttcat cttctgtgac
    21301 gtatacataa tcgacaagat ttcctgggat aacaacttca ttcggtttta gttgatagtc
    21361 gtcaactaaa gctttaactt gtacaataac tttcccatga ttggctttcg cgtttaatgc
    21421 gacatgataa cactcgctca agtacgcttc ttgagttaaa taaatgttac cttgttgatc
    21481 tgcgtatgtt cctctcagta gtgccacatc aacgctaggg aatgtgtaat gtaagtatgt
    21541 ttcatcgttg atggttacta atgaaactaa atcatccgtt gttcgtgtat ttactttacc
    21601 gccaccgtat ctaggatcaa cagctgtgtt taatccgatt ttagtaataa ctccaggtaa
    21661 taattgatta ctctgacgat aatgagttgc aatgatacct tgtggtaaaa aataagcttc
    21721 aatgtcatta tttttcattg cttgtgccgt tttggaagaa gccgttaaaa tactcataat
    21781 gacacgttta atcatgcgac gttctataaa atcatctaaa tccggtgcgg cacctaaact
    21841 atgaatatca ttcgctaata taaacgttaa atcattgggc gtatgatatg tgtcatgttg
    21901 cgctaacaca gcacgtagaa cttcggcggg taagttggct acagctaatg ctggtaaacc
    21961 aatcacatca ccatctttaa tgatatgttg taagtcgtgc catgtgattt gtttcaagca
    22021 agtcacctcc atcacatttg ataaaatata gcgtttttac actttgtgta aacccttaca
    22081 agaaatataa cataacgacg tttaaaatca attagaaata tctttttatt ctgataatag
    22141 acacagtata gacacatttt gacggtcgat aacaattgta atatcaaggg tttgtaatga
    22201 attgaatatc attaaaatac ttatataaaa atattgttcg gaatataaaa agttaaatag
    22261 gttttgattt ttaaatatga aatacaaagt gcccaatcga acaaagtatt tatattaaaa
    22321 tatggaaaat ccatcaatat taaattaaaa tagttttatt atgaaaagtg aaagtaggta
    22381 agtctatgga aggtcttaat catcgaagaa atacagaaaa agaagagaca acacaaacgc
    22441 aatcagttgc acctaataca ggtgaagagg ggatgtcatc agcaagtaca caatcaacta
    22501 agacgtccga catacataat gaatctatcg ataaacaaat ggaagctaaa gcgcatgaaa
    22561 cagcgcaaaa tacagattta aaaaacgaag caagaagttt atttgataat gcaaccaaat
    22621 caatcggtag actagcgggc aatgatgaaa gcttaaatct taatttaaaa gatatgcttt
    22681 ctgaagtatt taagccgcat actaaaaacg aagcagatga aatatttata gcgggtactg
    22741 ctaaaactac gccagcaatt tgtgacatat cagaagaatg ggggaagcca tggctctttt
    22801 ctcgagtatt catcgctttc acagtaacat ttattggatt atgggtcatg gcagcaattt
    22861 ttaataacac taacgcgatt ccgggtctca tttttatagg ggctttaaca gtaccattat
    22921 cgggtttgtt cttcttttat gaatcaaatg cgtttaaaaa tattagcatt tttgaagtta
    22981 ttatcatgtt ctttattggc ggcgtatttt cattactaag tacgatggta ttatatagat
    23041 ttgtcgtttt tagtgatcaa ttcgaaaggt ttggttcttt aacatttttc gatgcatttt
    23101 tagtaggatt agttgaagaa actggaaaag cactcattat tgtttatttc gtcaataaat
    23161 tgaaaacaaa taagattttg aatggattat taatcggtgc tgctattggt gcagggttcg
    23221 cagtttttga atcagcaggt tatattttga atttcgcttt aggagaaaat gtcccattat
    23281 tagatattgt cttcacacgt gcgtggactg cgattggtgg tcatttagtt tggtcagcga
    23341 ttgttggtgc tgcaatagtt attgcgaaag aacagcatgg ctttgaattc aaagatattt
    23401 ttgataaacg ctttttaata ttctttttat cagccgttgt tttacatggc atttgggata
    23461 catctttaac tgtacttggc agtgatacgt tgaaaatatt tattttaatc gttattgtgt
    23521 ggatacttgt attcatttta atgggggcag gtttaaaaca agtgaattta ctgcagaaag
    23581 aatttaaaga acaacagaaa aaagtagacg aataataatt aaagcttatg ttgctcatat
    23641 gtttgtgaca taagctattt ttataatttg tctttaaaag agtggaatag gaatactttt
    23701 tggagttaaa aaagtgtttc acgttaaaca aatagtgaca attagattta tataaaatga
    23761 acatgattca ctgaaagtat gtaataatca ttttattgaa attcatcaaa cagaaattaa
    23821 tacaatcata taagcaaatt aaaccacgcc ataatcatat tggatgactt cggcgtggtt
    23881 tttatagttg aagcagggct gagacataaa tcaatgtccc acactccctt atcgttcaat
    23941 cgttgttcga taatcgatta aatagatacc ttcaggtgtt actttataat ttttaacctt
    24001 agagttagca gcgactattt gatcgttgta agcaatataa ctgtttggta catctcgact
    24061 tgataattta ataatatcat tagaaatatt gtgacgttcc ttaacatcta cagtatgatt
    24121 caattgatta attaaatcat cgacgttgct attattgtag tctcctttat taatagcacc
    24181 atctttttta tatgcttgat taaagaaata acctgtatct ccacgaggaa ttgttccgaa
    24241 actatacatc gttgcatccc atgcagaacg gtcttttaag taaccttcta tgtcatcaac
    24301 acttttaatg tcgatttcaa tatttgcttt tttagcatct gattgtaata cttgcgcaat
    24361 tttcgatagc tctggacgac cgtcatacgt aattaactta atttttaaag ggtgttcttt
    24421 tgtataacca tctttagcta ataacatttt tgcttgttcg atattttgtt tggttaactt
    24481 aggttcttta atatatggaa ttttatcatt aaatggactc gttgcaggtt tcgcataacc
    24541 ttgataaata tgatctgcaa taccttgtct atcaatgata tgatctaatg cttcacgaac
    24601 ggatttagtc atttttttat tagtatgatt atacataagt aaagaagttc taaatccaga
    24661 ttcttttgac acttttaaat tttgattatt ttctatgtct tgaactttat taactgggac
    24721 atcagttatt aaatcatctt tttgagattc taaatttctg acgcgattat tgccgtcttc
    24781 ttggtacgtc acagtaatat gatcaagttt cggtttacct tgccaatagt ccttaaaatt
    24841 cgacaatgat atttttcgag attgcttata atcttttatt tggtaagggc ctgtaccaac
    24901 aggagtttga ttaacatctg atttagcatc tgtatcataa attgccataa aaggattagc
    24961 taattcagat acaagttcag ggtaagcgga gttggtttta attgtcagtt tttgaccttt
    25021 agcggtaatt gatgatattg gtaatgaata tttgaccaag tcgctttttt tcatgctatt
    25081 ttcaaggcta gatttcactt tttctgcagt caatttttga ccgttttgaa atttaatatt
    25141 atcttttaat tctatatcta acgttgtatc atttggttga tgatacgatt tcactaatgc
    25201 tttttctatt tttccttgat catttgtttt aaataatgat tctgcagcac caatcttaac
    25261 tggtacatct gtttcataag gtgcaataga ctttgttttt aacggtaacg aaatatttaa
    25321 gtctttgcca gatgaatgca ttgagccaca tcctgataac actaatactg ctgaaaatat
    25381 agttgctagt cttttaaact tcatttcatt aacactctct ttctaattac tatgtaaaac
    25441 ccaacaatta atattttaaa actttatttt gttaaagtaa aatgttgttc aagtttagta
    25501 attattaaag ttcaattaat tgtagtaatt atgcttttta aaaataaata ttagaaatga
    25561 agttagcgac atttatagtg attcacgata aacatatata actaagtatt gagcaactgc
    25621 tgtagtacta cagcttggtt atgtttagta tcttttgctg catataacaa tagaacatga
    25681 ttatgctgat ttacaatatc ctttaatttt tcaaaagcat ctttttgcgc atcctgatca
    25741 cgtaattctt tttcatattt ttctttaaaa gctccaaaaa gtttaggatc atgttggaac
    25801 cattgtcgca actcagtaga aggggcaatg tcttttaacc aataatctag gttagcagtt
    25861 cttttcgaaa tacctctcgg ccagactcta tcgactagga tacgaatagc gtcggtatta
    25921 tctttattgt cataaatccg tccaatatct acggtcatct tgtgaactcc tttcttatga
    25981 aattcagtga gcatacatca atgcatgttg tggtgggacg accaaataaa ttttgcgaaa
    26041 atatcatctc tgtcctactc ccaattaaaa aacagccatg acaaagtaaa gtcatagctg
    26101 ttttggtata gatgtcattt atttttacgt ttagttaaat acttcaaacc aactgcaaag
    26161 accgtgtacc cggctatggt ttgtatcaat gttttaatta aattattatt tttaacaata
    26221 atatttgcag taacaatact tacgaaatat aatgcaaata cttttacgta acgttgatta
    26281 agtttcatat gagcactaaa cctcttttct cagttatact gcaacgctta gtcttggaat
    26341 aaatgtttcg tagtgtacgc gatccatatc gtaatttaaa gatttaagtg cttcgatcat
    26401 agattgtaag aattttgtac caccacagat ataaatttca ggtttatttg ctaaaaatac
    26461 ttgtaattct tcagcaccaa tatagccttg tttatctttt aagtgtgtat ataatttagc
    26521 gttgtcatga tggcttgcga tactgttgaa gttgtctttg aaaggtaaat gttgttcatt
    26581 ttcagcaact tgaaccatct gtgtatctaa acctttggca gaggcagctt catacatagc
    26641 tactaaaggt gtaacaccaa tacctgaacc taagaaaagt tgtggttcag tcgtattctc
    26701 taatacgaat ccacctacag gcgcagctaa attaatcata tcgccttctt taatctcatc
    26761 gtgtaaaatt gttgaaactt cgccttcatg ttctgttgtg acatcacgtt taacgccaaa
    26821 agttaaatgg tttttttcac ctgatacgat agaatagtga cgtttagctc tatatggaag
    26881 tttatcacta gaaacatcaa ctgtgatgta ttggcctggt gtaaattcac taaagtcata
    26941 ttcttcagtt tcaactgtaa atgatttaat gtcttcagat tcttgtttaa tattggtaat
    27001 tttgaatggt ttaaaaccaa tccacatcat ttgatcataa atttcttttt caatttggat
    27061 gaacacatcc gcaataacgc catatgcttt tgcccaagct tgaatgacag ggtcattttc
    27121 ttctaatcct gtcacgtctt gaatggcttt taataaattt ttccccacaa ttggataatg
    27181 ttcagcataa acttgtagtg cgcagtgttt atatgcgact ggcataatga ctggtttaat
    27241 aacacttaag ttatcgatat taaccgctgc ggccattaca gcttgtgcta atgctgaaga
    27301 ttgcatgcct cgtttttggt tcgtttgatt aaacatgttt aaaagttcag gatgcgcttt
    27361 aaacattttt ggataaaaga ttgacgtaat ttctgtccct ttctctttaa gtaaaggcac
    27421 cgtttgtttg ataatgtctt tctcttgttc tgtaagcatg atactcctcc tttaatctgt
    27481 atattttgat tattctacta aaaatttcga tattcaatta attggtttga gaaaatataa
    27541 ataaaatggc aaatttgata attgtatgac atttttaatt ttttaaatac ttatcaataa
    27601 gcattgtgta caattgtctg tttgcacacc gacgattgag cgcatttatt tgactaattc
    27661 aaaaaacatt gttgttttcc tagaaaaaag taaacatgat aataaaaatg tgaaagtgta
    27721 aataatcact ggcgaagtac gaagactaaa gacatctaag atgtaatcgt atacaaatta
    27781 aaaaggtgta aaaattaaaa taaaatgtga aataaatcac aatttaatat tgacccagta
    27841 cttaatgcat gttacatttt atatgtgaaa taaatcacaa acttaaaagc ggatgacaca
    27901 tgacctttta agttatgcgt tgaaaataaa agagatgttt atttgctttt gtatcgtcaa
    27961 taagcagcat taaactaaca tatttgaagc tacatgtatg ttagtgaatt aatcataagg
    28021 gagtttttgt aatgaacaaa tttaaaggga acaaagttgt attaataggt aatggtgcag
    28081 taggttcaag ctacgcattt tcattagtga accaaagcat tgttgatgaa ttagtcatca
    28141 ttgatttaga cactgaaaaa gttcgaggag atgttatgga tttaaaacat gccacaccat
    28201 attctccaac aacagttcgt gtgaaagctg gcgaatacag tgattgtcat gatgcggatc
    28261 tagttgtcat ctgtgctggt gctgcacaaa aacctggaga aacacgttta gatttagtat
    28321 ctaaaaactt gaaaatattc aaatcaattg ttggtgaagt aatggcatca aaatttgatg
    28381 gtattttctt ggtagctaca aatcctgttg atattttagc gtatgcaaca tggaaattct
    28441 ctggtttacc taaagaacgt gttataggtt ctggtacaat tttagactct gcacgcttta
    28501 gattattgtt aagcgaagcg ttcgatgttg cgccacgtag cgtcgatgct caaattattg
    28561 gtgaacatgg tgacactgaa ttaccagtat ggtcacacgc taatattgcg ggtcaacctt
    28621 tgaagacatt acttgaacaa cgtcctgagg gcaaagcgca aattgaacaa atttttgttc
    28681 aaacacgtga tgcagcatat gacattattc aagctaaagg tgccacttat tatggtgttg
    28741 caatgggatt agctagaatt actgaagcga ttttcagaaa tgaagatgcc gtattgactg
    28801 tatcagcatt attagaaggc gaatatgagg aagaagatgt ttatattggt gttccagcag
    28861 tcatcaatag aaacggtatt cgcaacgtcg tagaaatccc attaaacgac gaagaacaaa
    28921 gcaagttcgc acattcagct aaaacattaa aagatattat ggctgaagca gaagaactta
    28981 aataactttt tataaaatct ataccatccc aaaaattgta aaaccttacc caaaaaattg
    29041 tataaagggc tatttgatac aactatatgt gtcgagtagc cctattttta atgtagtgaa
    29101 agtcgttgtt gaaattaaat tcaaatctgg cacttaagct ttaatcatag cattaagatg
    29161 attgtctagc agaggcgatt tgcgggctca ctacagtgca tgatgaactt aatgcttcaa
    29221 atgtaacatt aaaaataaaa gcaacgatgt cacttcttac ttcgtacatc gttgccatta
    29281 gttctgttga tatttcggtt agtcttaatc cccgagcaat tcttcaattt catttttgat
    29341 aactgtaacg tgaggcccat aaattacttg cacaccagtg ccttgctgga ttacaccttt
    29401 ggcaccagta ctttcgagta atactttatc gactttgtca ttttgatgaa gtgtgacgcg
    29461 tagtctcgtt gcacaacagt caacgatttc aatgttatct ttgcctccca aaccagcaac
    29521 aatagtttgt gctctttcag tagcctcaac ttgttgtgct gcagctttat cttctcgacc
    29581 aggtgttttg aaattaaatt tcgtaattaa gaatctgaaa acgatgtaat acaaacagaa
    29641 ccacacaatt ccaataggta tgacgtatag gtagtttgtt ttactattac cttgtagcac
    29701 accaaagagt aagaaatcga taaagcctcc actgaaggtt tgaccaattg taatgttgaa
    29761 aatgtctgcc atcataaatg ctaatccatc aaagaaggca tggattacat aaagaatagg
    29821 tgcgacaaac aagaaactaa actctaaagg ttcggtaata cctgttaaaa atgaagtgag
    29881 tgcagcggat aacattaaac cgccgacaac ttttttatgt tcaggtttag ctgtgtgata
    29941 aattgcaagt gcggcaccac ataagccgaa catcatcgta ataaaacggc ctgacataaa
    30001 gcgtgacaca cctgaataat acttcgtcac atctggatca ccaagttgag caaagaagat
    30061 gttctgcgta ccttgaacta agtgcccttt gacttctaaa gtaccaccaa gtgccgtctg
    30121 ccaaaacggt aagtaaaaaa tatggtgtaa accgagtgga cctaacaatc ttaagatgaa
    30181 gccataaaca aaagtaccga tggcacctgt tttcgttaca aatccaccaa catgataaat
    30241 gccggcttgt atgcttggcc aaatgaaaaa catcaataca cctaaaaaga ttgcggcaaa
    30301 tgctgtgaca atagggacaa atctagagcc accaaagaaa cctaaatacg gtggtaatac
    30361 cactttgtga tatttgttgt gaagtattgc ggtcataata cctgtgataa tcccgccaaa
    30421 aacaccggtt tcaaccgttt gtataccgag caccatgcct tgtccatttt gtgcaagctg
    30481 atcttttgcc aatgtgcccg tgatagttaa taagccattc atagttgcgt tcataattaa
    30541 gaaaccgagc agcgcagcta aacctgcagt acctttatcg cttctagata atccgattgc
    30601 gacaccaatt gcaaagatga ccggtaaatt ttggaaaaca atactacctg cagctgacat
    30661 taatgtaaaa atattttgta ataaggtaat atctaaaata gggtatgctt taacggtgtt
    30721 tggattactt aatgcaccac cgatacccaa caatagacct gcagctggta agattgcgat
    30781 aggtaacata aaggacttgc cgaactgctg tgctttttca aataaagatt tcatcaacat
    30841 ccctcctaat tattctcaat atagcttttg agaaatttaa tatcaatata tattctgtgt
    30901 atgaaaatat ttttcataaa aattgtttga atcatgtaac aatcatataa attgctgttt
    30961 atattgttgt gaaaatgagt tgacaaaagt cggtatagat atgtagacat cctattttta
    31021 gcgaggtagg ttgataaggc atatcggata aatttataag ccataaaata gataaatagt
    31081 gtttgtatcc aaaaatatga gaaagttaaa actatttttc aaaataaaca tgattcacca
    31141 cataaataaa tatacataag ctatcgataa caacttaaga aaggtggata tataaatgaa
    31201 aagaaagatt attatggatt gtgatccagg acacgatgat gcaatagcat taattttagc
    31261 gggggcaatt gacagtccac tagagatatt agctgtaaca acagtcgcag gtaatcaatc
    31321 agttgacaag aatacgacaa acgccttgaa cgtattggat attatgggac gccaagatat
    31381 agcagtagcg aaaggtgcgg ataggccgtt aattaaacca gctgcctttg cttctgaaat
    31441 acatggggaa tctggattag atggtccgaa actaccgtcg acaccatcac gtcaagcagt
    31501 tgcaatgcca gcatcagatg tgattataaa caaagtgatg acgagtgata cacctgtaac
    31561 aattgtagcg acaggtcctc ttacgaatgt agcaacggca ttgattcgtg agccaagaat
    31621 cgctgagcat attgaatcta ttactttgat gggtggtggt acatttggaa attggacgcc
    31681 tacagcagaa ttcaatattt gggtagatgc tgaagcagcg aagcgtgttt ttgaaagtgg
    31741 gattactata aatgtgtttg gtttagatgt aacacatcaa gttttagccg acgatcacgt
    31801 gattgaacgc tttgaaagta tcaataatcc tgttgcacag ttcgtcgtag aattattgca
    31861 attctttaag aagacataca agactcactt taatatggat ggtggtccaa tacatgatgc
    31921 ttgtacaatt ttgtatttgt tacaaccaga attgtttaca atggtacccg ttaatatcga
    31981 cattgaacat caaagtccac taacttatgg cactatggct gtcgatttaa atcatgttac
    32041 aggtaagcct gccaatgctt attttgctac agcagttgat gttgaagaag tgtggaactt
    32101 gatagaccat aagttacgta catacgaata ataatactta attaaataga tacagttaac
    32161 cctaaggcgc ctgatataag cgtccttagg gtttttgtat tgggttacaa tggtcatgac
    32221 aatttgataa tgattgagat atgtatgtta aaaatgcttc aaaaaaacac aaaacacaaa
    32281 aatatatgca aaaatttcaa ttgctttatt ttaaaagatt aaaatcacaa aaaatataga
    32341 tataattgaa atgttgttaa aacatcacaa tgatgtacat gctcattttg aaaatatgtg
    32401 ttcaaagtgt gtacatcggt gtgacgattg atgataaatt gagctcagat gctcaaagga
    32461 gaatcgaaca tgaacgggga taatcagcaa atactcagag aaattgtatt gaatcctact
    32521 attcatggta aagaacttga atcgatattt ggtttgtctc gtagacaact aggatatcgc
    32581 attcaaaaaa tcaatttgtg gcttgaacaa gagggttatc caaaacttga aagaacaagc
    32641 caaggaaatt ttattgtaag ttctgaaatc atgacgttat tcaagcgaga tgtatcggag
    32701 cagcaaatgt taaacggcaa caatgtcatt tttagcatag aaacacgtcg ttattattta
    32761 atgctcatgc tttttagtaa ggaaaacgca atgtctctaa accatttttc aattgattta
    32821 caagtcagta aaaatactgt cattcacgat ataaatcatg tgaaagagca attggaaaat
    32881 catggtttgt cacttaagta ttctcgaaaa catggttatg aaattgttgg tgatgaattt
    32941 gaagttcgcc gtttcttcat taagttgatt gatcaaaggt tgaatcatga tattactaaa
    33001 agtgaagttt taaaggcgct caacttaaca ttcgaagata tcgcatatca aaaagacaag
    33061 atcaaacagg tagaacaatt tttgaagagt cgctttatag acaaatcact tagttcattg
    33121 ccttatgtcc tttgtgtgat tcgtagacga attcaaagtg gtcatgtgat gaatccatta
    33181 aatattaatt atcagtattt gagggatacg aaagaatatc aagcaacgga gattatgacg
    33241 caacatgagc cggatttgcc agaagcggaa aagttatatt tgacattaca cttactttca
    33301 acaagtgtgc aatggactga tttgcaagaa tcagatagca tatcgaattt aacgatggcc
    33361 atcgctcaaa tgattcacca ttttgaacaa atcactttta ttaacattga agataaggag
    33421 aaattatcac agcaactctt gttacattta acgcctgctt tttataggat taaatataac
    33481 ttaacggatc gtgatgaatt aataaatcct ttacaaggaa attatcaatc cttatttcat
    33541 atggtgaaac aatcatgtca atcgttaact gaatatttcg gaaaatcgtt gcctgataat
    33601 gaaatagcat atttaaccat gttgttcgga ggtagtttga gacgtcaaga tgaaaacttc
    33661 gatggcaaga taaaagctat tatcgtgtgt acacaaggca cgtcagtatc acaaatgatg
    33721 ttatacgagt tgcgaaactt atttccagaa attattttct tagatgcgat ttcacttaga
    33781 acatttgaaa attacacatt agattatgac atcgtctttt caccaatgtt tgtcctaaca
    33841 cataaaaaat tatttatcac aaaagtagct ttatctgaaa atgagcaacg aaagttacgt
    33901 aaagaagtga tgaagtacat taataaggaa tcggctgaca ttgataagga aataaacaag
    33961 ttaatggcat taattgaacg cacaacgaca gttaatgaca ttacagaact acgtgatggt
    34021 ttagaagatt ttattgccaa ttataattca atttcaacca ttaatggatc gattgtcaca
    34081 caaaataaga cattagattt agctgacttg ataccggcaa ggcacgtgaa aagaatgcat
    34141 catgttgaaa atattgatga agctattgct aaagcaagtg atgtgttagt tgctaatcat
    34201 tttattgata ttaaatatat tcatgagatg caacaggtat ttgatgattc gtatatggtt
    34261 atcatgcaaa atattgctat tccacatgca tactctgaaa agcatgtaca taaaacagcg
    34321 atgagtatgt tgatattaca agaaccaata tacatgtcag atggcacagc aatccatatt
    34381 attgtaccta ttgctgctgt tgataaagtg acacacttaa gagcgttact acaattgaga
    34441 gatgtggcgc aagacaatga cgcaattaag cgcatcatac aaagtcgcaa aaattctgat
    34501 gtaaatgaga ttttaaaaaa ttattcaaat aaagaagcga gggaaaatgg atgggacagc
    34561 aattagtgca taaagaaaat ataatgctca atttgtcggc aactgataaa gaatccgtat
    34621 tgtcacaaat gtcagatgtg ttatttcaaa atgggttcgt gaagtcaacg tttaaagatg
    34681 cagtcatcga cagagaaaaa gaatttgcta ctggtttacc aacgcatcta tgttcggtcg
    34741 ctataccgca tacagatgtc gaacatatta accatagaac gataggtgtg gctgttctag
    34801 aaaaagaagt gccgtttatt gaaatgggaa cacttgatca acagacagaa gtgaaaatcg
    34861 tttttatgtt agcaatggat aaagtagatg atcaacttaa gctgttacaa cagttgatgc
    34921 aaatttttca aagtgaagaa aaattggagc agattctacg aacgaaagat gaaacaattt
    34981 tagcaacact aatcaatgat tatttggaat ataactaaaa attaattgga ggaattgaaa
    35041 atgaaacaag tattagtagc gtgtggtgca ggtattgcaa cgtcaacagt agtaaataat
    35101 gcaattgaag aaatggcaaa ggaacacaat attaaagtag atattaaaca aatcaaaatt
    35161 acagaagttg gaccttatga agacactgca gatttattag ttacaactgc aatgacaaaa
    35221 aaagaatata aattcccagt tatcaacgca cgtaatttct taactggtat tggtattgaa
    35281 gaaacaaaac aacaaatctt aacagagtta caaaaataac ggaattgata tgtaacgtgg
    35341 gtcgataaca tattaatata tgcaaattgc atataaatga ctatcacgac aaatgattgt
    35401 tgatgacata gaaacgcagt gactgtaatt caaaaggact gcagtcgaat ttagcaaggg
    35461 ttgtcgttga aatgactgta acgtcaaatg taatcacaat cggaaagtat cgtgacaatg
    35521 catatataac agggagggtt taaatatgag ttacttcact gattttgtaa ggggattttt
    35581 agatttaggt gcaactgtta ttttaccggt tgtcatattc ttgcttggcc tattctttag
    35641 gcagaaaatt ggagcggcat ttaggtctgg tttaacaata ggtgtggctt ttgtagggat
    35701 tttcttagtc atcgatttat tagttaaaaa tttagggcca gcagcacaag cgatggttaa
    35761 aaatttaggc gtcagtctga atgtgattga tgtaggttgg ccagcaacat catctatcgc
    35821 ttgggcatca tctgtcgcag catttattat tccactcgga atcatagtta acgttgtatt
    35881 gctagtaact aaagtgacaa agacgatgaa tgtagatatt tggaattttt ggcattatac
    35941 gtttacagca gcaatggttt atgccgtatc aggcagtatt tggcaagcgt tattagcagc
    36001 agttattttc caaattatct gtttgaaagt agcagattgg acagcaccga tgatgagtga
    36061 gttctttgat ttaccaggtg tatcgattgc tacaggaagt acaatttctt atgcaccagg
    36121 tatttactta gttaaattgt tacaaaaagt acccggtctg aataagttag atgctgatcc
    36181 tgaaacaatt caaaaacgtt ttggcgcatt tggagagtct atatttgtcg gcttgatttt
    36241 aggtttaggt attggtgtgt tagcaggtta caaacctgga gacatcatta atttaggaat
    36301 gtcaatggct gcagtaatgg tattaatgcc tagaatggta aaaatcttaa tggaaggttt
    36361 aatgccagtt tcagagtctg caagaacatg gctaaataaa cgttttggcg aacgtgaaat
    36421 ttatattgga ttggatgcgg ctgtagcatt aggtcatcca gcagttattt cgacagcatt
    36481 aattttagta cctatcactg ttttattagc cgttatttta ccaggaaacc aagtactacc
    36541 ttttggtgac ttagcaacga taccatttgt tgtcgcgttt attgttggtg cagcaagagg
    36601 aaacattatt cattctgtca ttgtgggtac gattatgatt gcaatttcac tatatattgc
    36661 aacagacgta gcacccattt tcacagatat ggcgaaaggt acgaatgtac aaatgccaaa
    36721 aggttcatct gaaatttcaa gtattgatca aggtggtaat atcgttaact atcttatctt
    36781 taaactattt agtctattca attaaaaatc gaggtgtttg tggtgaaagc tttagtaaaa
    36841 acaagagaag gacatggcaa cttagaactt cttgataaag aagttgcaac accgctagat
    36901 gataaagtaa agattaaagt acattatgca ggaatttgtg gcacagatat tcatacttat
    36961 gaaggtcatt ataaagttaa ttttccagtg acattaggtc atgaattttc tggtgaaatc
    37021 gttgaagttg gagcagacgt taaagatttt aaagttggtg accgtgtcac atctgaaacg
    37081 acattctatg tttgtaatga gtgtgaatac tgtaaatcaa aagactataa tttatgcaac
    37141 catcgaaaag gtattggaac acaagttgat ggcgcattta ctaattatgt cattgcacgt
    37201 gaagaaagtt tgcatcatat tccagacgaa gtatcgtatc agtctgcagc tatgacagaa
    37261 ccattagcat gtgcatatca tggcgtttct aagattcaag tcaattcagg cgatgtagca
    37321 gttgtaatgg gacctgggcc aatcggatta cttgtagcac aagtgttaaa aagtaaaggc
    37381 gcaactgttg tggtaactgg attggacaat gacaaagtca gattagataa agcagaagca
    37441 ttgcacatgg attatgtagt caatttacaa caaacagact taaaaacgta tatcaatgga
    37501 attacagacg gttacggtgc agatgttgtt gttgaatgtt caggtgcagt tccagcagca
    37561 cgacaaggtt tggatatttt acgcaaaaaa ggtttctaca gtcaaatagg tatttttaag
    37621 gatgctgaaa ttccatttga tatggaaaaa gtgattcaaa aagaaataac agttgttggt
    37681 agtagaagtc aaaagccagc agattgggaa ccttcattgc aacttatggc ggatggttta
    37741 gtaaatgctg aagctttggt gacaaaaata tatgatattt cgaaatggga cgaggcgtat
    37801 caacatttaa aatccggcga aggtattaaa gtattactta agccgctcga tttagatgaa
    37861 aatgaaggag agaattaata tggtagaatc aatgctaact tttatgcttg ggccattaag
    37921 acaaatcact gatttttata tggaacattt actcgtaagt aattccattg tcattgcagg
    37981 ttattttgcg acaggtattt ttaaaaagaa aaaagttgtg aattaaatca aatttgaggt
    38041 gatttacaag tgaaagcatt gaaattatat ggcgtggaag atttacggta tgaggataat
    38101 gaaaagccag tcattgaaag tgcgaatgac gttattatta aagtacgagc gactggcata
    38161 tgtggttcag acacgtcacg atacaaaaaa atggggccat acattaaagg tatgccattt
    38221 ggtcatgaat tttcaggtgt agtagatgcc attggaagtg atgttacgca tgttaatgtg
    38281 ggcgacaaag tgacaggttg cccagcaata ccttgttatc aatgcgagta ttgtttaaaa
    38341 ggtgaatatg cacgatgtga aaagttattc gtcattggct catatgaacc tggatcgttc
    38401 gcggaatatg tcaaattgcc agcgcaaaat gttttaaagg ttccagacaa tgttgattac
    38461 attgaagcag caatggttga gccatcagcc gttgttgcgc atgggtttta taaatcgaat
    38521 atacaacctg gtatgactgt tgcagtaatg gggtgtggca gtataggttt gttagctatt
    38581 caatgggcac gaatatttgg tgctgcacat atcatcgcta tagatataga tgcgcataaa
    38641 ctagatattg caacatcatt gggcgcacat caaacaatca attcaaaaga agaaaatctt
    38701 gagaaattca tcgaaaatca ttacgccaat caaatcgatt tagctataga atcatcaggt
    38761 gctaaagtta cgattggtca aatattgacg ctacctaaaa aaggtggcga ggtggtatta
    38821 ctcggaatac catatgatga tattgagatt gatcgcgttc attttgaaaa aattctgcgt
    38881 aacgagttga cagtatgtgg ctcttggaac tgtttgtcca gtaattttcc gggcaaagag
    38941 tggacggcaa ccttacatta tatgaagacg aaagatatta atgtaaagcc tattatttct
    39001 cattttttac cgttagaaaa aggcccggag acatttgata aattagttaa caagaaagaa
    39061 cgatttgata aagtcatgtt tacgatttat tagtatgcac ctttgaggac gaaaacgctg
    39121 gtatagttat agctatgaaa gtgcgaatgc cgtctggtct atagatacta tcgaaataat
    39181 tcatcttcga atatacgttg ataaatagcc ggtttacttg tgtgaaatat gcttgtgaat
    39241 cggttgtttt gcattttgta tacttaaaat gagatggcaa tatttgataa tttttaaagt
    39301 gaaaatcaag tacagccact taataagata aatttattat aatatatggt aaaatgatgg
    39361 cagtaataat gaatttgaaa aagagtaaac attaatacct ttaacaattt aatatcgtca
    39421 gagttaatga ttaactgcat ggcaaaacaa cttagaatgg tcagttacaa aaatacattt
    39481 ttataaaaaa ttatcacact attgtgacaa ctatctttgg attaataaaa gaggcaagtg
    39541 agcaataggt taggcttatg tgcgggcata ggtcagtaat gtataaatgg aaatgatgta
    39601 atgacagaat ggaggacaac atgatttatg caggtatttt agcaggaggt attggttcga
    39661 gaatggggaa cgtgccatta ccaaaacaat ttttagatat tgataataaa ccgattttaa
    39721 tccatacaat tgagaagttc attttagtga gtgaatttaa tgagattatt atcgcaacgc
    39781 cagcacagtg gatttcccat acacaggata ttttaaaaaa atataacatt acagatcaac
    39841 gtgtcaaagt agttgcaggt ggtacggatc gaaatgaaac aattatgaac attatcgacc
    39901 atattcgcaa tgtaaatgga attaataatg atgatgtgat tgtaactcat gatgccgtaa
    39961 gaccattttt aactcaacgt attattaaag agaacattga agtagcagca aaatatggtg
    40021 cagtagatac agtcattgaa gcaattgata cgattgtaat gtctaaagat aaacagaaca
    40081 tacacagtat ccctgtaagg aatgaaatgt atcaaggcca aacaccacaa tcatttaata
    40141 ttaaattatt acaagatagt tatcgcgcct taagtagtga acaaaaagaa atcttatcag
    40201 atgcatgtaa aatcattgtc gaatctggac atgcagttaa attggtacgt ggagaactat
    40261 acaacattaa agtgacaaca ccgtatgatt taaaagtagc aaatgccatt attcaaggtg
    40321 atattgccga tgattaatca agtatatcaa ctcgttgcac cgagacagtt cgacgtcaca
    40381 tataataatg ttgatattta tggtaatcat gtcatcgtaa gacctttata cttgtctatt
    40441 tgtgcagctg atcaaaggta ttacacaggt cgaagagatg aaaatgtact acgcaaaaaa
    40501 ttaccaatgt cattagttca tgaagctgtt ggtgaagttg tatttgatag taaaggggta
    40561 tttgaaaaag gtacgaaagt agtaatggta ccgaatacac ctacagagca acatcatatt
    40621 attgcggaga attacttagc ctctagttat tttagatcta gtggttatga tggttttatg
    40681 caagactacg ttgtgatggc acatgatcgt atcgttccgc tgcctaatga cattgatttg
    40741 agtacgattt catacacaga gttagtgtca gtaagttatc atgctataca acgatttgaa
    40801 cgtaaatcta tacctttgaa aaccagcttt ggtatttggg gtgatggtaa cttaggttat
    40861 attactgcta ttttgctacg taagttgtac ccagaagcta aaacttatgt atttggtaag
    40921 acagactata aattaagtca tttttcattt gtagatgaca tctttacagt aaatcaaata
    40981 ccagatgatc ttaaaattga tcatgcattt gaatgtgttg gaggtaaagg aagtcaagtt
    41041 gcacttcaac aaatagttga acatatttca ccagaaggca gtattgcttt gttaggcgta
    41101 agtgaattac ccgtggaagt gaatacacga ttagtacttg aaaaaggatt aacgttgatt
    41161 ggtagtagtc gaagcggctc taaagatttt gagcaagttg ttgatttata tcgtaagtac
    41221 ccagacatag ttgaaaagtt agcattatta aaaggacatg aaattaatgt atgtacgatg
    41281 caagatatcg tccaagcgtt tgaaatggat ttatcgacat cttggggaaa aacagtattg
    41341 aaatggacga tttaataaac cgatggggga ataacaatga caaaaacgaa acaagcaata
    41401 catattgata acatatactg ggaacgtgtt cagttatata ttgaaggaca tagtgaaggt
    41461 gtcgatttaa catcaggaca atttgttctg aggaatttaa ccgaaacaaa aacattagaa
    41521 gcaaatgaaa tgaaaataga cggtaataca tttatatgta gattcaacgt cgcaatatta
    41581 gacgatgggt attatttacc aatggataaa tatttatttg tttatcatga ccagttagag
    41641 tatattggac aacttaatcc aaatattatt gatcaagctt atgcggcatt aaatgaagag
    41701 caaattgaag aatacaatga gctgactaca caaaatggaa aagtgaacta tttattagcg
    41761 tatgatgcta aagttttccg taaaggtggc gtatcacaac atacggtcta taccattact
    41821 ccggaaatag caagtgacgt taacgaattt gtatttgata ttgaaatcac cttacctcaa
    41881 gagaaatcag gggtcattgc gacaagtgca cactggcttc ataaacaagg tcataaagct
    41941 tcatttgaaa gtagaagttt cttatttaaa gctattttta atattacaaa gttactacat
    42001 attaaaagaa gcaaaacaat attattcaca tcagattcgc gtccgaattt atcagggaat
    42061 ttcaagtatg tatatgatga gttactacgc caaaaagtag attttgatta tgatattaaa
    42121 acggtattta aggagaatat tacggataga cgcaaatgga gagacaagtt tagattgcca
    42181 tatttacttg gtaaggccga ttatattttt gttgatgatt tccatccatt aatttatacg
    42241 gttcgcttta gaccatcaca agaaattatt caagtgtggc atgctgttgg tgcctttaaa
    42301 acagttggct ttagtcgtac aggtaaaaaa ggtggtccgt ttatcgattc attaaaccat
    42361 cgtagttaca cgaaagcata tgtttcatca gaaaccgata ttccatttta tgctgaagca
    42421 tttggaatta gagaagaaaa tgttgtacca acaggtgtac cacgtactga tgtactattt
    42481 gatgaagctt atgcaacaca aattaaacaa gagatggaag atgaattgcc aattataaaa
    42541 ggtaagaaag ttattctatt cgcaccgaca tttagaggta atggtcacgg tacggcacat
    42601 tatccatttt ttaaaattga ttttgaacgt ttagcaagat actgcgagaa gcataatgca
    42661 gttgtgttat tcaaaatgca tccgttcgta aaaaatagac ttaatatttc acgtgaacat
    42721 agacaatact ttatcgatgt gtcagatcat cgtgaagtta acgatattct ctttgttaca
    42781 gacttgttga ttagtgatta ttcatcttta atatatgaat atgcagtatt taaaaagccg
    42841 atgattttct atgcatttga cttagaagat tacattacga cgcgtgattt ctatgaacca
    42901 tttgaatcat ttgttccagg taaaattgta cagtcctttg atgcattaat ggatgctttg
    42961 gacaatgaag attatgaggt tgaaaaagtt gtgccattct tagataaaca ttttaaatat
    43021 caagatggtc gctcaagtga acgtttagtc aaagatttgt ttagacgcta atgttggcat
    43081 atattacttg ccatgcaaat gagtcataag gcatagtttt caagaagggt tctgacaatg
    43141 aagtgagcaa catgtcatga tgagaagcag gactatacaa tgagaataac cttttgattt
    43201 ttcatgcata aggcgattca atcaaaagca atcaccttcc aactgaattg tcattttgta
    43261 aaataaaata atcgatccaa tcgttatcta attcataaat gtgtaaacac atacgttatg
    43321 aatggataac gattttttgt tatgttaaag tggtacatta atcatgtatt tcgtatgata
    43381 attaacgaca agtgtaatgg ttaaatgtat tttatgatga aatgctataa taggcatggt
    43441 tacaatgagc ttgctcatac atattaatat aattacaaaa acacgtcgga ggtacgacat
    43501 gattaaaaat acaattaaaa aattgataga acatagtata tatacgactt ttaaattact
    43561 atcaaaattg ccaaacaaga atctaattta ttttgaaagc tttcatggta aacaatacag
    43621 cgacaacccc aaagcattat atgaatactt aactgaacat agcgatgccc aattaatatg
    43681 gggtgtgaaa aaaggatatg aacacatatt ccaacagcac aatgtaccat atgttacaaa
    43741 gttttcaatg aaatggtttt tagcgatgcc aagagcgaaa gcgtggatga ttaacacacg
    43801 tacaccagat tggttatata aatcaccgcg aacgacgtac ttacaaacat ggcatggcac
    43861 gccattaaaa aagattggtt tggatattag taacgttaaa atgctaggaa caaatactca
    43921 aaattaccaa gatggcttta aaaaagaaag ccaacggtgg gattatctag tgtcacctaa
    43981 tccatattcg acatcgatat ttcaaaatgc atttcatgtt agtcgagata agattttgga
    44041 aacaggttat ccaagaaatg ataaattatc acataaacgc aatgatactg aatatattaa
    44101 tggtattaag acaagattaa atattccatt agataaaaaa gtgattatgt acgcgccaac
    44161 ttggcgtgac gatgaagcga ttcgagaagg ttcatatcaa tttaatgtta actttgatat
    44221 agaagctttg cgtcaagcgc tggatgatga ttatgttatt ttattacgca tgcattattt
    44281 agttgtgaca cgtattgatg aacatgatga ttttgtgaaa gacgtttcag attatgaaga
    44341 catttcggat ttatacttaa tcagcgatgc gttagttacc gactactcat ctgtcatgtt
    44401 cgacttcggt gtattaaagc gtccgcaaat tttctatgca tatgacttag ataaatatgg
    44461 cgatgagctt agaggttttt acatggatta taaaaaagag ttgccaggtc caattgttga
    44521 aaatcaaaca gcactcattg atgcattaaa acaaatcgat gagactgcaa atgagtatat
    44581 tgaagcacga acggtatttt atcaaaaatt ctgttcatta gaagatggac aagcgtcaca
    44641 acgaatttgc caaacgattt ttaagtgata acttaaaaac aataaaaaat tataaattaa
    44701 ttagttaagt gatataaata ataaacgaaa tgtttgct
//
LOCUS       DAKTLH010000007        43082 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.007, whole genome shotgun sequence.
ACCESSION   DAKTLH010000007 DAKTLH010000000
VERSION     DAKTLH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 43082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43082
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.007"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(274..1239)
                     /locus_tag="O0394_000298"
     CDS             complement(274..1239)
                     /locus_tag="O0394_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCY0498414.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
     gene            complement(1453..3090)
                     /locus_tag="O0394_000299"
     CDS             complement(1453..3090)
                     /locus_tag="O0394_000299"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCY0498415.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPDNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAENRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            complement(3250..4128)
                     /gene="hchA"
                     /locus_tag="O0394_000300"
     CDS             complement(3250..4128)
                     /gene="hchA"
                     /locus_tag="O0394_000300"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCY0498416.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            complement(4394..5581)
                     /locus_tag="O0394_000301"
     CDS             complement(4394..5581)
                     /locus_tag="O0394_000301"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCY0498417.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWNNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
     gene            5752..6927
                     /locus_tag="O0394_000302"
     CDS             5752..6927
                     /locus_tag="O0394_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0498418.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTFKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            complement(7209..8393)
                     /gene="tuf"
                     /locus_tag="O0394_000303"
     CDS             complement(7209..8393)
                     /gene="tuf"
                     /locus_tag="O0394_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCY0498419.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(8610..10691)
                     /gene="fusA"
                     /locus_tag="O0394_000304"
     CDS             complement(8610..10691)
                     /gene="fusA"
                     /locus_tag="O0394_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCY0498420.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            complement(10814..11284)
                     /gene="rpsG"
                     /locus_tag="O0394_000305"
     CDS             complement(10814..11284)
                     /gene="rpsG"
                     /locus_tag="O0394_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCY0498421.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            complement(11350..11763)
                     /gene="rpsL"
                     /locus_tag="O0394_000306"
     CDS             complement(11350..11763)
                     /gene="rpsL"
                     /locus_tag="O0394_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCY0498422.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            complement(11861..12115)
                     /locus_tag="O0394_000307"
     CDS             complement(11861..12115)
                     /locus_tag="O0394_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HCY0498423.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            complement(12252..15848)
                     /gene="rpoC"
                     /locus_tag="O0394_000308"
     CDS             complement(12252..15848)
                     /gene="rpoC"
                     /locus_tag="O0394_000308"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCY0498424.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIHHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            complement(16012..19563)
                     /gene="rpoB"
                     /locus_tag="O0394_000309"
     CDS             complement(16012..19563)
                     /gene="rpoB"
                     /locus_tag="O0394_000309"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCY0498425.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            complement(19778..20386)
                     /locus_tag="O0394_000310"
     CDS             complement(19778..20386)
                     /locus_tag="O0394_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0498426.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            complement(20561..20929)
                     /gene="rplL"
                     /locus_tag="O0394_000311"
     CDS             complement(20561..20929)
                     /gene="rplL"
                     /locus_tag="O0394_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCY0498427.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            complement(20972..21472)
                     /locus_tag="O0394_000312"
     CDS             complement(20972..21472)
                     /locus_tag="O0394_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCY0498428.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     misc_feature    complement(21510..21656)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(21744..22436)
                     /gene="rplA"
                     /locus_tag="O0394_000313"
     CDS             complement(21744..22436)
                     /gene="rplA"
                     /locus_tag="O0394_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCY0498429.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
     gene            complement(22644..23066)
                     /gene="rplK"
                     /locus_tag="O0394_000314"
     CDS             complement(22644..23066)
                     /gene="rplK"
                     /locus_tag="O0394_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCY0498430.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            complement(23247..23795)
                     /gene="nusG"
                     /locus_tag="O0394_000315"
     CDS             complement(23247..23795)
                     /gene="nusG"
                     /locus_tag="O0394_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCY0498431.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            complement(23808..23990)
                     /gene="secE"
                     /locus_tag="O0394_000316"
     CDS             complement(23808..23990)
                     /gene="secE"
                     /locus_tag="O0394_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCY0498432.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFR"
     gene            complement(24046..24189)
                     /gene="rpmG"
                     /locus_tag="O0394_000317"
     CDS             complement(24046..24189)
                     /gene="rpmG"
                     /locus_tag="O0394_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCY0498433.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            complement(24304..24873)
                     /locus_tag="O0394_000318"
     CDS             complement(24304..24873)
                     /locus_tag="O0394_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCY0498434.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRFDVENNYLNKLAIKELICQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            complement(24954..25478)
                     /locus_tag="O0394_000319"
     CDS             complement(24954..25478)
                     /locus_tag="O0394_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCY0498435.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            complement(25478..26224)
                     /gene="rlmB"
                     /locus_tag="O0394_000320"
     CDS             complement(25478..26224)
                     /gene="rlmB"
                     /locus_tag="O0394_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCY0498436.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLLTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            complement(26232..26636)
                     /locus_tag="O0394_000321"
     CDS             complement(26232..26636)
                     /locus_tag="O0394_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCY0498437.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            complement(26629..28029)
                     /locus_tag="O0394_000322"
     CDS             complement(26629..28029)
                     /locus_tag="O0394_000322"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCY0498438.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            complement(28013..28660)
                     /gene="cysE"
                     /locus_tag="O0394_000323"
     CDS             complement(28013..28660)
                     /gene="cysE"
                     /locus_tag="O0394_000323"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCY0498439.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            complement(29083..30537)
                     /locus_tag="O0394_000324"
     CDS             complement(29083..30537)
                     /locus_tag="O0394_000324"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCY0498440.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            complement(31094..32167)
                     /locus_tag="O0394_000325"
     CDS             complement(31094..32167)
                     /locus_tag="O0394_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCY0498441.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            complement(32192..33556)
                     /gene="radA"
                     /locus_tag="O0394_000326"
     CDS             complement(32192..33556)
                     /gene="radA"
                     /locus_tag="O0394_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCY0498442.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEVLSF
                     ALHS"
     gene            complement(34041..36497)
                     /locus_tag="O0394_000327"
     CDS             complement(34041..36497)
                     /locus_tag="O0394_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCY0498443.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            complement(36511..37518)
                     /locus_tag="O0394_000328"
     CDS             complement(36511..37518)
                     /locus_tag="O0394_000328"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HCY0498444.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            complement(37508..38074)
                     /locus_tag="O0394_000329"
     CDS             complement(37508..38074)
                     /locus_tag="O0394_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HCY0498445.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            complement(38093..38554)
                     /locus_tag="O0394_000330"
     CDS             complement(38093..38554)
                     /locus_tag="O0394_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCY0498446.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            38712..39926
                     /locus_tag="O0394_000331"
     CDS             38712..39926
                     /locus_tag="O0394_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCY0498447.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            complement(40134..40694)
                     /gene="pdxT"
                     /locus_tag="O0394_000332"
     CDS             complement(40134..40694)
                     /gene="pdxT"
                     /locus_tag="O0394_000332"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HCY0498448.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(40698..41585)
                     /gene="pdxS"
                     /locus_tag="O0394_000333"
     CDS             complement(40698..41585)
                     /gene="pdxS"
                     /locus_tag="O0394_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HCY0498449.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            41689..43071
                     /locus_tag="O0394_000334"
     CDS             41689..43071
                     /locus_tag="O0394_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCY0498450.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITVKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHV
                     DALIKSLIIK"
BASE COUNT        12983 a         8357 c         6306 g        15436 t
ORIGIN      
        1 attttgctgt tcaacaaatt ttctaaaaat tcaaaattaa ttaacagatt tctagaaaga
       61 ctatatcttt tagtataaac gtattaattt cacagagaca agtaatctgt gttttactaa
      121 tatactttac atacaaaaaa ctctttactt taaaatgaac taagctcgcg aattcaataa
      181 gtataatgaa taatattaga attcatgcac tagtttatta aaataaagag taatttaaaa
      241 tatcattccg tgtattaaag tgaatggaaa tgattagtta ttatttttaa cagtatcttt
      301 ttgttcaata gcttctaaca ttaatttagt catgctcgct aaatcatatt taggatcaaa
      361 tccccattcg ccacgtgaac agcttgtatc aatagaatcc ggccaactat tagcgatacc
      421 ttgtctaata ggatcaacat cgtaatctaa tgtaaaattg ggatagtatt cttgaattgc
      481 ttcttttacc atctctggat caaaactcat tgcgctcaaa ttataaccat ttctagtttc
      541 taatttagcg tcgtctgctt ccataagttt aataattgct tcaattgcat catccatata
      601 catcatatcc atatacgtgc ctttatctat gaagcttgta taatgaccct ctcttactgc
      661 tttgaagtat atttcaacag catagtctgt agtaccgcca cctggctctt taacatgcga
      721 gattaaacct gggaatctaa cacttcttgt atctacacca aaacgtttga aatagtattg
      781 acacaataat tctccagcta ctttatttac accatacatt gtcgtaggtt gctgaatcgt
      841 tacttgtggc gtattaactt taggagttga gtctccaaat gcaccaattg aacttggtgt
      901 gaaaaagtgc aaattataag ttcttgcagc ttctaatgca ttcattaatc cacccatatt
      961 taaatcccaa gctagaattg gatttttctc agcagttgct gataataatg ctgccatatg
     1021 cattagacta tccgcttcaa agtccctaac taactcaaac atacggtcac gatctgttac
     1081 gtctaagatt tcaaatggtc cattttgtac aggtgagtct gcttcaggtt ccctaatatc
     1141 tgtagcaaga acattatctg tcccataaat ttctctgcac ttaacaacta attctgtacc
     1201 aatttgtcct aatgcaccag taatcataat ttttttcata gaaatatctc ctttgtctct
     1261 tctatatagc tatagtccat cacaagcgga cataatattc attttcataa taattataat
     1321 ataaaagcgc tttcttgtat atatgacatg tacatgttgc tgatatttct gtaaatggaa
     1381 attctagttg tattaattga ttttagtaat ttatagcgtt tattattgct aattactgtc
     1441 aaattaaatt ttttatccct caactcttaa actctggata tctttcatta tattagcttt
     1501 tttataacca tggatatcat gtaaagcctt ataagcttta aataatgttt catacctttg
     1561 tactttttcc gcttctggat taatttcgta taaaattggc tctttcatag catcaactgc
     1621 gtcattaatt gaattatgtg cgccaccact gactgcacct aatattgctg cgcctattgc
     1681 tggtgcatac tcactatcca taacaattag ttttttattc agtacattcg catagatatc
     1741 catcataaca gcattctttt taggtatacc gccacatgca aatacctttt ctacttccat
     1801 atgccaatct tgatactgtt gcataatcat cttagtacca aatgctgtag cttctaaata
     1861 tgctctataa atatcctcat gcttagtttg taaagttaat ccaaagatac aacctgttaa
     1921 attgctatca cttaatacac ttcgatttcc attatgccaa tcaagagcaa tgagcccact
     1981 ttcacctggc atttgatgtt ttatcttttc attcattaat tcaaatacag tcatatttct
     2041 attttctgct tcatctacat atgactttgg tgcttgctta gcgacatact caaacaaatc
     2101 acctactgct gattgccccg cttcataagc aaataattct ggaataattg ctccttttac
     2161 agaacctgat atacctggca cttgatgttg cttttcattt aacataagat ggcatgtgct
     2221 tgttcccatc accatagtca tttctttatc tttttcagac ccaataccta ataaactagc
     2281 atgggcatca ataataaaag gacttaccat agtttctttt gataatccta atttctgtgc
     2341 cattttatca tccagtttcc ctactgcttc accaatatta acaaccggtg cagatacttt
     2401 atcttgaatt acttttgata atttggggtc tattttatca aataaatcat aatgaaaccc
     2461 tgtttcttct tcccaaaatg ctttgaaacc taatccacaa ttcgagcgta catttttatt
     2521 agttaattta tttacaatcc aatcgcccgc ttccataata taagccgttt tttccataat
     2581 ttctggtgct cgattcatga cctccattat tttgggaatc atccattcac tactaacatt
     2641 atatccataa tggcctaacc acttattatt attttcaata gcagtttgat ataatttctc
     2701 tgcctcttta tatgcaccat gatgtttcca aagtttcaca tacgcatgtg gattgttttt
     2761 aaattgtttt aagttatgta ccgggttaag gttttcgtca gtaaaaataa tagtagatga
     2821 agtaaagtct ataccaatac ctactatatt gtctggatct atttttgatt cacgtactat
     2881 atatgatatt ccttcttcca taatttctaa ataatcatta ctattttgaa gtgcatatgt
     2941 atgtggtatt ttcaaaccat ttaattcact ctcaatgaca ccatgtgtat atggtttcac
     3001 aaattttgat actacttgac cgttagttgt attaattaaa aacacacggc ctgaagcagt
     3061 tccataatca attccaatgc tataagacat catgtttcct ccatatcgct agttgcggtt
     3121 attagttttg caagcgtttt cttaaatcat actcataata ggttctattt tacaagtatc
     3181 acttaaattt tgccaaaaaa aaagagccct catacattta catatgaggc tcctctaatt
     3241 aatatttaat tatttatttt gtattgcatt taacatttca ttaactgcta attttcctaa
     3301 ctcatttgaa gctaaaggac tgtcacctgt taataattta cgatctttta acgttcttcc
     3361 tgtcatatcg tcgttaacta cttttaatcc ttgtttagtt aataaatcag caactaacca
     3421 tttcaagcgt ccaggtaaat aacctatttc aatatttgca ccttcatcta atgagtcagg
     3481 gaagacacaa acagagtatc cttctaatgg agatttttct ctgttaagcc ctgctgaaag
     3541 tagtgctgct ggtccatgac ataatgtaac tataaagcgg tcattgtcta atgcccaatc
     3601 taatgtttgt tgaacgtcct cactttcaga aataccaaca actgcagcat gtccgcctgg
     3661 gataaagaca gataaatagt ctgaatcagg tcctaattca tttttaatca catctgctaa
     3721 tttttttggc tgttttaatt tttctttcaa tttattataa gtacttataa ctgcctcgtc
     3781 ttcagttggc atagcccata attctaattt aactggataa ccagataatg tcgcaacatc
     3841 aacgtcaaaa cctgcttcca ttaaatgatg taaaggtaat aacatttcaa caggatgatt
     3901 acccgtagag aacatttttc cattttccaa taatacatat cgctcttcag cagcaatcat
     3961 caatactttc catttaccat ctttataggc acctttgtgt tcaacaccat caaaatctgt
     4021 tttaggtgct gtatattgac taagggaata tggtgatggg aaaaatgcgt tatcttctgc
     4081 tttatctggc gttggttgct tacttaattc atttacatct tgtgacatag tttattccta
     4141 ctttctctat atagttacta ctagtataga catatactac acactataaa catgtattac
     4201 tattaaaatg ctcattatca aaaataatca aaatgaattt ctttgaaaaa aatacaacta
     4261 aattctacaa gatgctttta aaattcaaac ttcaattttg gggagcacta aacattgata
     4321 tacgaactta ctgttaataa actaaacatt aaaaaaagcc cacgacaata tttgccgtgg
     4381 gaataaataa atattaaatc aacttcattt cttttcctac tttttcataa gccgcaattg
     4441 cttcatctaa catgtctttt gtatgtgcag ctgtaggcat atttcttaca cgtcctgtac
     4501 ctcttggtac tgttgggaaa acgatagatt tcacatagac accttcgtct tttaaacgct
     4561 tactaaattc ttgagttgtt ttttcatcac caataattac tggtgtaatt ggagtttctg
     4621 actcacctgt atcatatcct aattttgaca atccattttt taaatattgt gcattgttcc
     4681 ataatttatc atgtaattca gttgaatcca ttaacttttt aactgcttca gttattgctt
     4741 tggtatcccc aggtgctaat gatgtagaga ataagaatgg tcgtgattgt gcttttaacc
     4801 aatctattaa ctcttttgta cctgctacat aaccgccaac gacaccaatt gctttagaaa
     4861 gcgtacctat ttggaaatcg attttatctt gtaaaccaaa atgtttaacc gtaccagcgc
     4921 ctttacccat aacacctgaa ccatgagcgt cgtcaacata agttaataaa ccaaattctt
     4981 ctgcaatttc tacaatttca ggtaatttag ccacatcacc atccatacta aaaacgccat
     5041 cagtgatata catcacttta ttgtattgac ctgattcaac tgcttctttt gctttcgcac
     5101 gtaaatcatc catgtctgaa tggttaactc gaataatttt agctttagat aagcgacatc
     5161 catcaataat tgatgcatga ttaagctcat ctgataaaat agcatcattt ttattcatga
     5221 cagctgaaat agcagccata ttacaattaa atcctgattg ataagctatt gcagcttctg
     5281 ttcctttaaa ttttgctagt gtttcttcta attcgtcgtg taaatctaat gtaccattga
     5341 ttgtacgaac agcgcctgca cctacaccat gtgtatcaat agctgctttt gcagctgatt
     5401 tcaaatcttc atttgttgct agtcctaaat aattatttga agataagtta atgtatgatt
     5461 tcccattgat tttgatttct ggtccgtttg caccttcaat tgtatctatt tcattataca
     5521 aaccattttc ttttagataa tttatatttt cctctaaaaa ctcatgtaat gattgaacca
     5581 cagtatataa tcccccttaa gtattgtttg gtaatacctt tatcatacag taattctaca
     5641 taacttgaaa gccttatcat tcatagataa aatatctcaa agcaatcatt tagtacgata
     5701 atttcataaa atgttatact ataaacatta ttttagaaag tagtgataag ggtgttagat
     5761 tggttccaac tagcaaataa taaagaaaat aaaacaattc aactcagacg ttatttacac
     5821 caatatcctg agttatcttt tgaagaattt caaacacatg attatattgt taaccaatta
     5881 agccagttat cttgtgatat tgaaactcct atcggccgca atggtatcaa agccacattt
     5941 aaaggcttgg gcacaggacc taccattgca ttaagagctg actttgatgc gttacctgtc
     6001 gaggaattaa acgatgtgcc ttataagtct aaaaatccag gatgtatgca tgcgtgtggg
     6061 catgatggtc atacagcaat tttacttact gtagcagaga ttttagatga acataaacat
     6121 ttattagaag gtaatgttgt attaatattc cagtacggtg aagaaatcat gccaggtggt
     6181 tctcaagaaa tgatagatgc tggatgtctg gaaaatgtag atagaattta cggtacacat
     6241 ttatggagtg gttatcctac tggaactatt cattcacgtg cgggggcaat catggcctct
     6301 ccagatgaat ttagcgtcac atttaaaggt cgtggtggtc atggtgccaa acctcatgaa
     6361 accattgacc caatagtaat catggcagag ttcattttaa gtgcacaaaa aataatttca
     6421 cgtacgatcg atccagttaa acaagctgta ctttcatttg gaatgattca agcaggcacc
     6481 acagatagcg ttattcctga tcaagcattt tgtaaaggta cagttagaac tttcgattca
     6541 gatatacaga atcatgtaat ggacaaaatg gataagcttc tacaaggttt agcaatagca
     6601 aatgatatta actatgattt gaattatatt aaagggtatt tgcctgtcca taataacgag
     6661 aaagcatatc aagttattaa agaagcaaca aatgacttac atgtacgctt taatgaatca
     6721 gatttaatga tgattggaga agatttttca cattatttga aagtacgacc aggtgcattc
     6781 tttctgactg gttgtggtaa tgaaagtaaa ggtattacag cgccacacca taatcccaaa
     6841 tttgatattg atgaaaaatc attaaaatac gctgtagctg tatttttaaa aatcatagaa
     6901 ttagagcaag tatttaaaac aaattaagca tttaaattaa ttgaaagata tgaatctgag
     6961 acataaacta atgaaatgct cccttcaaag tagacattga aaaaatgaaa actttgaagg
     7021 gagcatttta caatgtccaa gactcaacaa ttttatttaa acagtattat cagttataag
     7081 ttttattagt cagtctttca tcaattattg ttcttctaaa tcgaatagcc attacaaaat
     7141 ttacacatta aaaaaagacc cctcgattga gggatcttct ttttatataa aatctaagaa
     7201 attagaaatt atttaatgat ttcagtaaca acgcctgatc ctacagtacg tccaccttca
     7261 cggattgaga aacgagtacc gtcttcaatc gcgattggag cgattaattc tactgtcatt
     7321 tcaacgttat caccaggcat taccatttca gtaccttctg gtaagtgaac aacaccagtt
     7381 acgtcagtag tacggaaata gaattgtgga cgatagtttg agaagaatgg agtgtgacgt
     7441 ccaccttcgt cttttgataa tacgtatact tctgctttga attcagtatg tggtgtaatt
     7501 gaaccaggag cagctaatac ttgaccacgt tgtacgtctt cacgagcaac accacgtaat
     7561 aatgcaccaa tgttgtcacc agcttcagcg tagtctaata atttacggaa catttcaaca
     7621 cctgtaacag ttgttttaga tgtgtcatgt aaaccgatga tttcaacttc ttcaccaact
     7681 ttgatttgac cacgttcaac acggcctgta gcaacagtac cacgaccagt gattgagaat
     7741 acgtcctcaa ctggcatcat gaatggtttg tcagaatcac gttctggagt tggaatgtaa
     7801 gtatctacag cttccattaa ttctaagatt ttttcttcgt attgagcatc gccttctaaa
     7861 gcttttaatg ctgaaccagc gattacaggt acatcgtcac ctgggaagtc atattcgctt
     7921 aataagtcac gaacttccat ttctactaat tctaataatt cttcatcgtc aaccatgtca
     7981 actttgttta agaatactac taatgctggt acaccaacgt tacgtgataa aagaatgtgt
     8041 tcacgagttt gtggcattgg accgtcagca gcagatacta ctaagatacc gccgtccatt
     8101 tgagcagcac cagtgatcat gtttttaacg tagtcagcgt gtcctgggca gtcaacgtga
     8161 gcgtagtgac gtttgtcagt ttggtactca atgtgagaag tattgattgt gataccacgt
     8221 tctttttctt ctggagcgtt gtcaatcatg tcatatgatt gtgcaactga gtcaccattt
     8281 tttgctaata cagtagcgat tgctgctgtt aatgttgttt taccatggtc aacgtgaccg
     8341 atagtaccga tattggcatg ttctttagaa cgatcgaatt tttcttttgc cattataaaa
     8401 tctctcctat tctaattaaa aataatattt aaatttatct ctcatgatag tttctcacca
     8461 tcatgagaaa ttatttatag cgatgcaaaa agaattttac aattctttta taatgcattc
     8521 accccaaaaa atcaaactat aaagatgata gcttacattt aagctcacct tgtattttaa
     8581 cctaggctag ctagtcaaaa caagttatat tattcacctt tatttttctt gataatatct
     8641 tcagcgattg attttggaac ttcagcatag tgatcgaagt acatagtgta agtaccgcga
     8701 ccttgagtgt ttgaacgtaa tgatgttgca taaccgaaca tttctgaaag tggtacataa
     8761 gcattaacaa cttgtgcatt accacgaggt tccataccat caacacgtcc acgacgagat
     8821 gttacgtcac ccatgatatc acccatgtac tcttcaggca tttcaatagt tactttcatc
     8881 attggttcta agattacagg atcacatttt ttagcagctt ctttaagtgc taatgatgca
     8941 gcaattttga aggccatttc agatgaatcg acatcatggt atgaaccatc atataattta
     9001 gctttaacat caattaaagg ataacctgct aaaacaccat tttccatagc atctttaaga
     9061 ccagcttcta ctgatggaat gtattcacga ggaactacac caccaacgat agcgttttcg
     9121 aattcgaaac ctgcgcctgt ttcgtttggt gtgaattcaa tgtgaacatc accgtattga
     9181 ccacgaccac cagattgacg agagaattta ccttgaactt gtgcagatga tttgaatgtt
     9241 tcacgatatg aaaccattgg agcacctacg ttacattcaa cgttgaattc tttcttcata
     9301 cggtctacta agatgtctaa gtgaagctca cccataccac cgatgataac ttgtccagtt
     9361 tcttcgtcag tgtgtgcatg gaatgttggg tcttcttctt gtaatttaac taaagcttga
     9421 gtcattttat cttggtcagc tttagatttt ggctctactg ataagtgaat aactggctct
     9481 gggaattcca ttgattccaa gataatgtca tttttctcac cacataaagt atcaccagta
     9541 cctgtatctt taagacctac cgcagcagcg atatctccag agtatacagt atcgatttct
     9601 tgacgtgagt tagcgtgcat ttgtaataaa cgacctacac gttcacgttt acctttagta
     9661 gagttcttaa cgtatgaacc agatgtcatt gtacctgaat acacacggaa gaatgttaat
     9721 ttaccaacat aagggtcagt cataactttg aacgctaatg cagcgaattc agctgaatcg
     9781 tctgctttcg cgattacttc ttcttcaggg ttgctagcac ggtgaccaat aattggttta
     9841 acgtctagtg gtgaaggtaa gtaatcaatt acagcgtcaa gcattaattg aacacctttg
     9901 tttttgaaag ctgtaccaca aagtactggg tagaattcta cgttagtagt agcttggcgg
     9961 atagcttctt ttaattcaga aactgaaatt tcttcgtcac caagatattt ttccattaat
    10021 tcgtcgctag tttctgcaac tgcttcgatt aagctagcac gagcttcttc agctctatct
    10081 aagtggtctt cgggaatttc aatttcttca atttcagtac ctaaatcatt tgtatatttg
    10141 aaacatttca tttcaactaa gtcaatgatt gcttcgaatt cgtcttccgc accaattggt
    10201 aattggattg gagcagcgtt agcttgtaaa cgatcatgta atgtacttac agagtattcg
    10261 aagttagcac ctaatttgtc cattttgttt acaaatacga tacgtggaac accataagtt
    10321 gtagcctgac gccaaactgt ttcagtttga ggttcaacac ctgattgtgc atcaagtact
    10381 gtaactgctc cgtcaagtac acgtaatgaa cgttcaactt ctacagtgaa gtctacgtgt
    10441 ccaggtgtat cgataatgtt tacacggtga ccttcccaag ctgctgttgt tgcagcagat
    10501 gtgatagtaa taccacggtc ttgttcttgc tccatccagt ccatttgtga agcaccttcg
    10561 tgtgtttcac caattttgtg gatacggcca gtgtaataaa gaatacgttc agtcgtagtc
    10621 gttttaccag catcaatgtg agccatgata ccgatattac gagttttttc taatgaaaat
    10681 tctctagcca tgtatttttt ctccttccag tttatattac tgaataaata cgatagatat
    10741 ggcgatgccc taagcatgaa cgcttaatga aaattcatta atatagaaca cactcagggt
    10801 aaaagctttt atcttaccaa cggtagtgag caaatgcttt gtttgcttca gccattttgt
    10861 gagtgtcctc acgtttctta acggcaccac ctgtattatt tgctgcatct aaaatttcgt
    10921 tagctaaacg atcttccatc gttttttcac cacgaagacg tgcatagtta actaaccaac
    10981 gtaaacctaa agtagtacga cgctctggac gaacttctac tggtacttga tagttagaac
    11041 cacctacgcg acgagcttta acttctaata ctggcataat gttgttgatt gcttcttcga
    11101 atacttctaa tgcatcacga ccactgcgtt gttcaactag gtcgaatgct gaataaagaa
    11161 ttctttgtgc tgttccacgt ttaccatcta acataatttt gttaattaat ttagttacta
    11221 acttagagtt atgaattgga tctggtaata cgtctctttt aggtactgat cctttacgag
    11281 gcataatgta aatcctccct tccttatatt aaatatttta agtttaagat ttaattaaaa
    11341 actaaattct tagtttttag gtttcttagt tccgtataat gaacgacctt gtctacgtcc
    11401 gtcaacacct gaagtatcaa gtgctccacg tacaatatgg taacgcacac ctggtaagtc
    11461 ttttacacgt ccaccacgta caagtacaac actgtgttct tgtaagttat gtccgatacc
    11521 agggatgtat gcgttaattt cgatgttgtt tgataaacgc acacgtgcat atttacgtaa
    11581 cgctgagtta ggttttttag gtgtcattgt acctacacga gtacatacac cacgtttttg
    11641 tggtgagttt aagtcagtaa attttttctt tttactgttg aaacctttat ttaaagctgg
    11701 agaatctgat tttttgattt tgctttgtct tggtttacgt actaattggt taatagttgg
    11761 catgtgatat gtcctcctct cttcactttt taatcccaca catccaggtg gttcattttt
    11821 aggttaaata aaatttagta agtaaaacac ttactaattc tcatttaatc aatgctacta
    11881 ttgtcgcatt gacgttaata cctacatgtt tacccaaagc atgtttgctt ttgaaaaaag
    11941 atacaggtat atttttctga ttgatttggc ttaacacgcg agtcattaaa tatacttcaa
    12001 cgtcttcagc aataatcaaa gatgtaactt gatctttctt taacgcttta agcgtttctt
    12061 taagaccaac tacaaaatgt tgtttgttaa agcgtgcaac tttttcctta gacaataatt
    12121 tatcctccaa agttctgctt gcatcaacct ttaatatatt aacattgaac aagagaattc
    12181 gtcaacatta aatcaaataa tttacataaa aataacaaga ggctaaagca ttagcctctg
    12241 ttatatactt gttattccgt tacttcagtt tgagattcaa cttctgcaac tggtttagct
    12301 gttttttcgt attttacgtc gctataacgt ctcataccag taccagctgg aattaactta
    12361 ccaataatta cgttttcttt aagacctaat aagtcatcac gcttaccttt aattgctgca
    12421 tctgtaagaa ctcttgttgt ttcttggaat gatgctgcag ataagaaact ttctgtttca
    12481 agtgatgctt tagtaatacc aagtaatact ggtttagctg ttgcaggacg cttacggtgt
    12541 ttaaatgctt ctctatttgc atctgtaaag ttatgaatat caactaatga acctggtaat
    12601 aacttcgtat cacctgcttc gataattcta actttacgta acatttgtcg aaccataacc
    12661 tcaacgtgtt tatcgtcgat ttctacacct tgcatacggt aaactttttg tacttctttt
    12721 aataagtagc tttcagtcgc gtttaatcca gcaacagata agtaattctt aggttcaata
    12781 gaaccttcag ttaatacttc accacgttga actggttgac cgatttctac aataattctt
    12841 gaagtacctg aagcaaggta tgatcttgtt tcattagcac ctttaacaac aatttcttgt
    12901 tgtctatctt ttgctaattt aatatcttct acgacacctt cgatttccgt aattaccgct
    12961 tgacctttag ggttacgtgc ttcgaaaatc tcttgaatac gaggaagacc ttgtgtgata
    13021 tcgctacctg ctaccccacc tgtatggaat gtacgcattg taagctgtgt acctggttca
    13081 ccgatagatt gggctgcaat tgtaccaact gcttcaccaa cttcaacttt ttcaccagta
    13141 gcaaggtttt taccgtaaca tttttcacaa acaccatgtc gtgcgttaca agtaaatgct
    13201 gagcgaatat acatttgttc aataccagca tctgtaattt tcttagcaat ttcaggtgta
    13261 attaattcat caggacgaat gattatttca tcagtttcag gatgatgaat tgtttcttta
    13321 gaataacgac cttcaatacg ttcgataaat ggttcaatca tttctgtacc ttctttaata
    13381 tcagaaacta ataaacctct atcagtacca cagtcttctt cacgaacaat aacatcttgt
    13441 gccacgtcaa caagacgacg agtaagatat cctgagtcag ctgttttaag tgctgtatcg
    13501 gcaagacctt tacgtgcacc gtgagttgag atgaagtatt caagtactgt taaaccttca
    13561 cggaatgaag atgtgattgg taattcgata atcttaccag atggtgcggc catcaatcca
    13621 cgcatacctg ctaactgtgt aaagttagat gcgttaccac gggcacctga atcactcatc
    13681 atgaagattg ggttagtttt atcaagtgat tgcatcaatt caccttgaat ttgatctttt
    13741 gcatctgtcc aaatttcaac aactgcatta tatctttctt cttcagtgat taaaccacgg
    13801 ttgaattgtt ttgtaattct gtcgactaat ttttcatgct catcaagtat ttgttgctta
    13861 tcaggtaata ctacgatatc agcaacacct actgtaatac cagctttaga tgagaatttg
    13921 aatcctaagt ctttcatacg gtctaacatc attgatgtat cagtgatgct aaatctgttg
    13981 aatacttctg caataatatt acctaagaat tttttgttga aaggttcaat taattcttca
    14041 ttttcaaagt attcttttaa tccaccttca cctaaagttg taggatcgat gaaatatctg
    14101 tttggtgtct ttctttctaa gttttcttgc gtaggttcat taatataagc aaatgaatct
    14161 ggaatgattt cattgaatat aattttacct actgacgtag caagaatctt tttgttttgt
    14221 tcttcagtaa atgttggatt attgaacgaa cttgcatgta caccaattct agtgtgtaaa
    14281 tgtacaaagc catttgcata tgcttttaat acttcatttg tattattaaa gattgcgcct
    14341 gtatttactg catcttttct ttctaaagta aggtaatagt taccaagtac catatcttgt
    14401 gatggtgtaa ctacaggttt accatcttta gggttcaaga tgttttgtgc tgctaacatc
    14461 aacattcttg cttcagcttg tgcctctttt gataaaggaa cgtgaaccgc catttggtca
    14521 ccgtcaaagt cagcgttata agctgttgtt acaagtggat gtagacgaat cgcacgacct
    14581 tcaactaaag ttggttcaaa tgcttgaata ccaagtctat gaagtgttgg tgcacggtta
    14641 agtaatacag gatgttctct aattacttct tccaatacgt cccaaacttc atcatccata
    14701 cgttcgattt tactcttcgc atttttaatg ttagttgcaa tttcacgttg aactaattct
    14761 ttcattacga atggtttaaa tagttcaagt gccatttctt ttggtaaacc acattggtac
    14821 attttcaagc ttggacctac tgcaataact gaacgtcctg aatagtcaac acgtttacca
    14881 agtaagtttt gacggaaacg accttgttta ccttttaaca tatgagataa agattttaat
    14941 ggacggttac ctgggccagt aactggacga ccacgacgac cattatcaat taaagcgtca
    15001 acggcttctt gtaacatacg tttttcgttt tgaacgatga taccaggtgc acctaaatct
    15061 aataaacgtt tcaaacgatt atttcgatta attacacgac ggtataagtc gtttaagtca
    15121 cttgttgcaa atcgtccacc atctaattga accattggac gaatttctgg tgggatgatt
    15181 ggaagtacat ctaaaatcat ccatgaaggt ttgttacctg aattacggaa tgattcaaca
    15241 acttctaaac gtttaattgc acgagtaagt ctttgaccag tagctgattc caactcatcg
    15301 cgtaacaatt taagttcttc gtcaagatca atctcttcaa gtaaatcttt aataccttct
    15361 gcacccattt ttgcaacgaa ttgacctggg tatttatcat aataatctct gaattcagct
    15421 tcagataata aagttttctt ttctaaacca gttggacctg gatctacaac aacataagaa
    15481 gcaaagtaaa taacttcttc taatgctctt ggtgacatgt caagtaataa tcccatacga
    15541 cttggtatac ctttgaaata ccaaatgtga gaaactggag cagcaagttc aatgtgaccc
    15601 attctttcac gacgtacttt agatttagtt acttcaactc cacatctgtc acagaccatg
    15661 cctttgtagc gaacacgttt gtatttacca caactacatt cccagtcttt tgtaggtccg
    15721 aaaattcttt cacagaatag accatctttt tcaggtttta atgtacggta gttgattgtt
    15781 tcaggttttt taacttcacc aaaagaccaa gaacggattt tttcaggtga agccaatcct
    15841 attttcatat aatggaaatt atttacatca atcaaggagc ctacctcctt caatttagat
    15901 tagctgtgct atttgattga tttgcattta tttaaaatgg cttaaagtac taaactgaat
    15961 catcaataaa attcagctta gtatcttttt gcctgttttg taaattgcgt attaatcagt
    16021 aacttctttt tgtgtttcag gagcatcatt ttgttgtaaa tctactttgc gttctacaac
    16081 atcatcgtca tcaacgtctg tcatttcgat ttcattatct tgctcatcca taacttttac
    16141 atctaaacct aaactttgta attctttcat caatactcgg aatgattctg gaacacttgg
    16201 tctagagatg ttttcacctt taacaatagc ctcgtatgtt ttcacacgtc ctactgtatc
    16261 atcggatttg taagttaaga tttcttgtaa tgtgtatgca gcaccatatg cttcaagtgc
    16321 ccatacctcc atctcaccaa aacgttgtcc accgaattgc gctttaccgc caagtggttg
    16381 ttgtgtaaca agtgaatatg gtcctgttga acgcgcatgt aatttatcat caaccatgtg
    16441 cgcaagtttc aacatgtaca ttacacctac tgaaatacgg ttatcgaatg gttcacctgt
    16501 acgtccatca taaagtacag ttttaccatc acgagccata ccagcttctt caattgttga
    16561 ccatacatcg tcatcgtttg caccgtcaaa tactggtgat gcaacgtgaa taccaagatt
    16621 tttagcagcc atacctaagt gtagctctaa tacttgtccg atgttcatac gagatggtac
    16681 accaagagga tttaacatga tatcgatcgg acgtccatct ggtaagtaag gcatatcttc
    16741 ttcaggaaca atcttagaaa tgacaccttt gttaccatgt cgaccacaca tcttatcacc
    16801 aacatgaatt ttacgttttt gaacgatata tacacgtact aattggttta caccaggtga
    16861 taatgtatcg tcgccttctt cacgattgaa tacttttaca tcaagaacga taccgccagc
    16921 gccgtgaggt acacgtaatg aagtatctct aacttcacgt gctttttcac caaagattgc
    16981 atgtaacaat ctttcttcgg cagttaactc agttacacct ttaggcgtta ctttaccaac
    17041 taaaatatct ccatctttta cttctgcacc aatataaacg ataccacgat cgtctaagtt
    17101 cttaagtgca ctttcagaaa cattaggaat atctcttgtg atttcttcag gtcctaactt
    17161 agtatcacgt gcttctgatt catactcttc aatatgaata gaagtataca cgtcatcttt
    17221 cacaagtctt tcactcatga taacggcatc ctcatagttg taaccgtccc aagtcatgaa
    17281 accaactact acgtttctac ctaatgccat ttctcctaat tccatagatg gtccatctgc
    17341 taaaatctcg ttatactcaa caacatctcc aactgcaacg attggacgtt ggttgtaaca
    17401 tgtacctgag tttgaacgtt taaatttagc taatggatag cgatctaatt caccttcatg
    17461 ctcaacgccg ttctcttcaa ctagacgacg aacaagaatt tcattagatt caacatgttc
    17521 aacacgacct ctgtgcttag ctgtaatagc cgcaccagaa tcacgtgctg caacgtgttc
    17581 catacctgta ccaacaaatg gtgcttctgg attcatcaaa ggcactgctt gacgttgcat
    17641 gttcgcaccc atcaatgcac ggtttgagtc atcattttct aagaatggaa tacatgctgt
    17701 cgctgctgaa acaacttgct tcggcgatac atccatataa tccatttttt ctttagccat
    17761 aactgtattg ttaccacgga aacgacatac aacttcatca tccatgaaac gaccattttc
    17821 atctaattta gagtttgctt gtgctacaac atagctatct tcttcgtcag ctgttaaata
    17881 gtcaatttga tcagtgatag catgtgtatc taaatcaact ttacgatatg gtgtttcaat
    17941 aaagccgaat tcatttacac gtgcataact tgataatgag ttaatcaatc caatgtttgg
    18001 tccctcaggt gtttcaattg gacacatacg gccatagtga gagtagtgaa cgtcacgtac
    18061 ttccatttga gcacgttcac gtgttaaacc accaggtcct aatgctgata gacgacgttt
    18121 atgcgttaac tcagctaatg ggtttgcttg gtccatgaat tgtgataatt gagagctacc
    18181 aaagaattct ttaatagatg caataacagg tcgaatatta attaattgtt gaggtgtgat
    18241 agactcagta tcttgaattg acattctttc acgtacaact ctttccattc ttgataaacc
    18301 gatacggaat tggttttgta gtaattcacc tacagaacgt aaacgacggt tacctaaatg
    18361 gtcaatatca tctgtatatc caataccgct taataagtta aagaagtaac tcattgaagc
    18421 aatgatatct gctggtgtaa tgcatttaac ttctgagtca gggaaagcat taccaattac
    18481 agttgtcgta cgaccttcat catcgttagg aacatatact ttaattgatt gaatttctac
    18541 tggctcgtct ataacgctac catgcaattc aaacacttcg ctgtttgcat ttgattcaag
    18601 tacatccatg atttcgtcga ttttacgacg atcaagcact gtaccttctt caactacaat
    18661 ttcaccagtt tcagtattta caattggctc agctaatttt tgattaaata aacgatgttt
    18721 taaatgtaat tttttgtttg ttttataacg acccacgctt gctaagtcat agcgttttgg
    18781 atcaaagaaa cgtgaataca atagactttt agcattttca acagttggtg gttcacctgg
    18841 acgtaaacgt tcatagattt ctaataacgc ttgttcagtg ttttcagtgc cgtctttctc
    18901 taaagtatta cgtaaatatt cattgtcacc taaaaggtca acaatttctt ggtcgcttga
    18961 gaaacctaat gcacgtaaca atactgttaa tggtagttta cgtgttctat caatacgtac
    19021 gtatacaaca tctttagcat ctgtttcata ttctaaccat gcaccacggt ttggaataat
    19081 tgttgcatca tagttttcac gaccattttt gtcgattttt tcattgaaat aaacggatgg
    19141 tgaacgaact aattgagata cgattacacg ttctgcacca ttgataacga acgtacctgt
    19201 atcagtcatt aatgggaaat cacccataaa gacttcttgt tctttaactt ctcctgtttc
    19261 tttaatgatt agacgcactt ttacacgaag aggtgcagca taagtagcgt cacggttttt
    19321 agattcttct aaatcatatt ttggttctcc taaacggtaa tccacaaact ctaatgacaa
    19381 attaccagta aaatcttcaa ttggagaaat gtctctaaac atttcgatta aaccttctct
    19441 taggaaccac tcgtaagatt tagtttgaat ttctattaag tttggtaatt ctaatacttc
    19501 tgaaattctc gcgtagtttc tacgtttacg atgtcttcca tattggacaa cttgacctgc
    19561 caaacagatt cacccctcaa aaattatgtg atccgctttt agctaaatac cataagcata
    19621 ttaataaaaa gacaaaaaga aaacggcaac actaatgatg acaccatttt cgattctata
    19681 tttattctta attaacttat ctatttaacg taaaagtaca cacttattga acataaattt
    19741 ttacattata taactataac agaatatcca tttcatttca agcttttata cttctcagaa
    19801 tgtaatatcc tttatcttta tttaccactt ctacatttcc aaaaagttca ttcattcttt
    19861 tctttgcaga tggcatacct tgcttcttct gaattacaac gaatagttca ccgttcgagt
    19921 ctaatctatg taatgcttgc tcgaatatac gatgcacggt ttctttccct gctcttattg
    19981 gtggattggt taaaataaaa tcaaaacttt tgtcttccac agcagacaaa gcatcacttt
    20041 cctttacgat cacattatca ataccattta attttttgtt tttttcaact aaggctagcg
    20101 ctctgtgatt aacatctagc attgtaattg aatgatgtgg tgatacttta gcaatcatca
    20161 aaccaattgg tccgtaacca caaccaacat cggcaattcg cttacttgga ccaggtggat
    20221 gcgcttttaa aaaagtttga acaagaacat ctgaaccata atctacttta tctttcgaaa
    20281 acactccgtt atcagttatt aaatcaattt tatgatggtt taattgatat tgaatacgtt
    20341 gttcattgct aattacactt ggatcttcat cgtaataatg actcattatt ccacctcatc
    20401 ttcactttgt aactcgtcat tataattata acatttcact cagtctttaa atgaataaaa
    20461 ccccgttatc aaaataacgg ggttttaact tctcaaagaa tcattatatt atagcaatac
    20521 ttgctataaa actttgatta ttaagtttaa gatacttgaa ttattttaat tctacagtag
    20581 ctccaacttc ttctaattgt tctttaagtt tttcagcttc ttctttaggt aaagcttctt
    20641 tgattacttt aggagctccg tctactaatt ctttagcatc ttttaatcct aaaccagttg
    20701 cttctttaac agctttaaca actttgattt tagatgaacc agctgaagtt aactcaacgt
    20761 caaattcagt tttttctgct gcagcgtcag cgccaccagc tgcacctgct actgctactg
    20821 gagcagctgc agttacacca aattcttctt caattgcttt tactaagtcg tttaattcta
    20881 atactgacat ttctttaatc gcttcaatga tttgttcatg attagccatt ttataattcc
    20941 tccattattt ttaatttttt tacgctaaaa attattcagc gttttcttct ttttgttctc
    21001 caatagcttt aaccgcataa gcgaagttgc gtacaggagc ttgtaatact gataaaagca
    21061 tagatacaag accatcgtgt gaaggtaatg aaccaacagt tttaacttct tctgctgtaa
    21121 taacattgcc ttccataacg cctgatttaa tttctaatgc ttcatgatct ttagcaaatc
    21181 cagaaattac tttcgctgca gctacagcat cttcacttga agttgcaata gcagtaggac
    21241 ctgttaagaa ttcatctaag ccttcgatac cagctttttc agctgcacga cgtaccatag
    21301 tgtttttgta tactttatac tcaacaccag cttcacgtaa ttgtgaacgt aagtcagtaa
    21361 cttcagctac tgttaatcca cggtagtcaa cgattactgt tgaaactgaa tttgatagta
    21421 cctcagcaat ttcatcaact agttgttttt tagcttcaat gatagcagac attcagacac
    21481 ctccatttaa atttttggtg ctttttttaa aattaataaa aaaagcactt tctacccacg
    21541 gcaaaaagtg cttgaaagat catagatcat cacgttcaag tcaattttag cctcggcagg
    21601 ataaatttta agttataaat aactcctact gtcttaggta aaataatcac aataagtaat
    21661 ataacttgga aattacttat tgtcaatata tttttacaga ctgaaagata tttctttcag
    21721 cctgtaattg tttatatcat ttattatttg aaacttgcag tatcaatttt aactccagga
    21781 cccattgttg tagttacagc aacagatttg aagtatgtac ctttagcaga tgatggttta
    21841 gctttagcta atacatcttg taaagtattg aagttttcaa ttaattgttc atcagtaaat
    21901 gaaactttac caattgatgc atgtacgata ccagcttttt cagcacgata ttctacttta
    21961 ccagctttga tttcttcaac agctttttta acatccattg ttacagttcc agttttaggg
    22021 tttggcatta aaccttttgg tcctaataca cgacctaatt taccaacttc acccatcatg
    22081 tctggtgtag caactactac atcgaagtcg aaccaacctt gttggatttt ttgaacgtat
    22141 tctgcttcac ctacatagtc agcacctgct gcttcagctt cagcaatttt gtcaccttta
    22201 gcgaatacta atacactttg tgatttacca gttccgtttg gtaatacaac tgcaccacgg
    22261 atttgttggt catttttacg tgtatcaatt cctaaacgga atgcaacttc aacagaagcg
    22321 tcaaagttag caatgcttgt ttctttagct aatttaattg cttcttcaac actgtagtgc
    22381 tgagtacggt caactttact agctgcttct tgatactttt tacctttttt agccatttat
    22441 agttcctcct ttagtggttt tagcggaatt tcctcccaca ttacttgcta tatgcaagtt
    22501 aagagcagac aacagaagaa ttaatcttat gaataaatca atcattaatc tagtcattca
    22561 gattatattt ataattcatc attattatct ctgttatctg cttcatcgtt taaatcatgt
    22621 aatcaaattt aatattcgta aaattattct acaacgatac ccatactacg tgcagtacct
    22681 tcgataatac gcatagctgc ttcttcgtca gcagcgttta agtcttgcat tttgctgtta
    22741 gcaatttcgc gtacttgatc tttagttact gtagcaactt tagttttgtt tggttcgcct
    22801 gaaccttttt caataccagc tgctttttta agtaatactg gagccggtgg agtttttgta
    22861 ataaatgtaa atgaacgatc ttcataaaca ctgatttcta ccggaataat taaacctgct
    22921 tgatcttgag tacgtgcatt gaactcttta cagaatccca tgatgttcac acctgcttga
    22981 cctaatgctg gaccaactgg tggtgctgga ttcgctttac ctgcaggaat ttgtaattta
    23041 acaactttat ctactttttt agccacgatg tgcacctcct tgatatcgtg atgtggtcac
    23101 agggctcagt tttgccctcc cactcttaac atttcgtgac gaaatgggcg ctctaaaagc
    23161 gcgaccacat aattttaaca cttttctaat taaaaaatca atactctttt ttatcttttg
    23221 gttagtttaa taactttaat tgttaattaa agcttttcta tttgatcgaa ttcaacttct
    23281 actggtgttt ctcggccaaa catatctact aatactgtta gcttaaactt atctgtttca
    23341 atttcttgaa cttcaccaac ttgattcgca aatggacctg atttaatacg aacttgctcg
    23401 ccaacttcga gttcaacatc gatagtcttt tctttaagac ccatttgttt taagatgaag
    23461 cgaacttctt ctggtaacaa tggatttggc ttagaccctg cacctgcaga acctacaaaa
    23521 ccagtaacgc ctggtgtatt tcttaccaca taccatgatt catctgtcat gattaattcc
    23581 actaaaacat atccagggaa tgttttttta acagtcgttt tagctttgcc atcttttact
    23641 tgagtttctt cttcttccgg tatgactact ctaaagattt gttcagtcat attcatagat
    23701 tctactcttt tttctaaatt ctttttaact ttattttcat atccagaata tgtatgcact
    23761 gcataccaac gctttgcgcc aacttcttca gacatgtctt cactcctcta acgaaataat
    23821 aaatttttca atgctgtaat tcctaaatct aaggcataga aaaagactaa gaagaatata
    23881 acagtagaaa ctacaattac agtatattta aatagctctt ctttcgtcgg ccaacttgtt
    23941 ttttccattt cagacttaac gcctttaaag aaactttctt ttttagccat ctgctcaacc
    24001 tccaaatcct ttgaacatat tatcaaagta ttatttcgaa tgtatttatt tcgattcttt
    24061 atgaattgtg tgcgcgttac attttggaca atatttcttt aaggttaatc ttgttgccga
    24121 gccttcttgc ttaggaacat tataatttct attgccacaa gcttcacaat ttaaaggtat
    24181 ttttctcaca ataattcacc tttattcatt ataataccag tatactatac ataaaattta
    24241 taacaaatgt caaaccatca gtaagtaaca cgttataatt caattcacgt cctaaggcgc
    24301 ttttcaaatc attttgaaat aacgttttat tttattttta catcgttgta tggcattata
    24361 aatcactttc tcttttacat gcatcaattg agcaatttct ctcggcttat attgttcaca
    24421 cattaaatac atgacatctt tttcaaatgc actcaaatac ttaaactgac aaatcaactc
    24481 tttaattgct aatttattca aataattgtt ttcaacatca aaacgcttta ttgcacatgg
    24541 atacgtaact ctatattcat taatcaaatt atccattctt ttatttgtag ctaaatattt
    24601 tctcaaataa tcatacttta cagacgtaat taaacattga acataatttg tgaaaggctg
    24661 ctctccacta aaatcaaatg tttggaccga cctatacatc cgtataagta tttcttgata
    24721 taagtcttct aaatcataat gattaaatcc aaatgttttg atgcgtcgaa taattaatgg
    24781 ttgtagatcc attactaact tttcgaagtc tttatcattt attgcattgt tacgtttgat
    24841 tgtactgtct tgagttgtca aatcgtattt caaatattaa actaacccct tctatctaaa
    24901 atttaaggtt agtttaatat tgttacattc aaaatttcaa gatgacggaa atgtcatttc
    24961 ttatgatgtc ctcttcgtat tttttcaaat tctgcaagga tttcagaaga taacggaatt
    25021 cgagttcttg gcttgttttc acttatatca tctaatgatt tactcacatc aatttcattt
    25081 tcttttaaat ctctccacat ttcgcgagat gatattctat atgcacctga tccaaagata
    25141 gcatgttgct cactcatatc acttgttaca actgtaatat gcttagtatg cttgtcataa
    25201 agttcataaa cataacgttc tatgaaacta tcagctgttt ctttttcctt ggtaaaaatc
    25261 gttttaacgc catgatacat gtattctctt tcaacacccg attggtcata agcatcgaaa
    25321 acacaaataa tttcatctga aataactgca ttataatttg caattgcatc tattaattgc
    25381 attctagctt cttctaaatt ctcttttgca atggcgctta gcgttggtga ttgtcctatc
    25441 atattgtatc catcaatgat taagtaacgt tctttcatta tatttctcca acatcatgtc
    25501 tttttcgaaa tacttcgtac atcattaaac ttgctgcaac cgaagcattc aaactgttta
    25561 catgtccaac cattggaatc ttaatataaa aatcgcattt atcacttact aggcgactca
    25621 taccctgtcc ttcgctacca attacaatag ccaatgacat gtccgcttct agatttctat
    25681 aatctgttgc attattagct tcagtgccag ctacccaaaa gccattatct tttagttcat
    25741 cgatagtttt agctaaattt gtcactcgaa taactggtac atgttcaatt gcacctgttg
    25801 aggcttttgc aactgtttgc gttagtgtaa ctgaacgacg tttaggaata ataacaccat
    25861 caactcccgt tgcatcggct gttcttaaaa ttgatcccaa gttatgtggg tcttctaagc
    25921 cgtctaatat aagtactgtc aataaacctt ctttttcttt ttgctgtttt aaaaattgat
    25981 cgaagtcagc atattcatat ggtgcaataa gcgctgcaac accctgatgt ggtgcatttg
    26041 ctaaaaaatc taatttagat tttggtacag tttgaacaat gattttttga tcttttgcat
    26101 tttttaaaat ttcattaatt tgttgctttt taataccttc ttgaatcaat atcttattta
    26161 tcggatgccc agtaataatc gcttctctaa cagcatgcct accaacaata accgtatctt
    26221 ccacatcgtc actacctttc gtttactatt gttattattt tatttaataa tgcctctaat
    26281 cgttcttctc ttttttctaa ataaagaaaa cctatcactg cttctatcgc tgaactttta
    26341 cgatatgttt gaacatcagt gtttttagct ttagtatgac ttttcgcgtt acgccctcgc
    26401 ttcaaaatat ccatttcttc gtctgtaaac cattcttgct ccattaaata ttctaacgtt
    26461 tgcgcctgac ttttggcaga tacatatttt ttagacattt gatgtagttt attaggctta
    26521 cttttaagct ttaaaacgat ataggtacgt acatattgat ctaagactgc gtctcccata
    26581 tatgctaagg tcaatggatt caataattta atgtgattat cttgttgatt atccacgttt
    26641 aaatctaacc ccttgaggtg tgtcttctaa tataatgttt tgtgatttca gcatgtctcg
    26701 aatttcatct gctcgtgcaa aatctttgtt tttccttgct tcattacgct cttcgattaa
    26761 tttttcaaca tcttcatcca ataattcatc tgcattttta gattttaacg gtacacctaa
    26821 aacatcgctg aaaatttgat aaactgcttt aaatttatca attacttctg ttgatgttgt
    26881 gttctctagt acatatttat tcgcaagttt tgctaaatca taccaagctg taattgcatt
    26941 agctgtatta aaatcatcat tcataactgt ttcaaaacga tttaaaatcg catcaatttg
    27001 atcaatatat gtctgttgat tttcaatatt agtagcaatt tgtgcgcgct cttcaattaa
    27061 ttgataacta ttgcgaatac gctctagtcc actacgtgct gattctacca attctagatt
    27121 atagttaatt gggcttctat aatgtacgct aatcataaag aatcttagta catctggatc
    27181 aacttcttta ataatatcgt gaactaaaat aaagttgcct agtgatttac tcattttttc
    27241 attatcaata ttaatgaaac cattatgcat ccaataatta gcaaatggcg catgattatg
    27301 tgcttctgat tgtgctattt cattttcatg atgtggaaat tgtaaatctg aaccacccgc
    27361 atgtatatca attgtaggtc ctagctcatg aaatgccatt acagaacatt ctatatgcca
    27421 tcctggtcta ccttcaccaa atgggctatc ccaactaatc tcgccaggct tcgctttttt
    27481 ccacaatgta aaatcaagtg catcttcttt atgctctcct gcatctatac gagcacccac
    27541 ttttaagtca tctatggatt gatgacttaa tttaccataa ccttcaaatt tacgtgttct
    27601 aaagtaaaca tcgccaccac tttcatatgc ataaccttga tccaccaaat ctttaataaa
    27661 ttgaataatg tcatccatat ggtccattac ccttggattt gaagtcgctt ttctaacatt
    27721 taacgcacca acatcttcat gaaaagcagc gatatatttt tctgcaattt cgggaacaga
    27781 ctgatttaat tcttgagaac gtttaattaa tttatcatct acgtctgtaa aatttgatac
    27841 atattctaca ttatatcctt ggtattcaaa gtaacgtctc actacgtcat aattaattgc
    27901 tggtcttgcg ttaccaatat gaatgtagtt atatacagta ggaccacata catacatttt
    27961 tactttccct ggttctatag gcttgaacac ttctttttga cgtgtaagcg tattatataa
    28021 tgtaatcatc ttgaatctct ccattcctag tcttttcaag ttgtcgttct aaatgcttaa
    28081 tttgttcata aattggatca ggtagatggc gatgatcaaa tgtttttcca actcgaacac
    28141 catcttgctt aacaatatgt cctggtatac caacaaccgt tgaataactt ggaactgatt
    28201 gtaaaacaac tgaatttgca ccaatattta catttgaatt tattttaata tttcctaaaa
    28261 ctttcgcacc ggctgctatt aaaacattgt ctcctatatc tgggtgtctt ttccctcttt
    28321 ctttccctgt cccaccaagt gtcacgcctt gatagattgt cacattatca ccaattgtac
    28381 atgtttctcc tattacaacg cccataccat gatctataaa tagacgcttt ccaattttag
    28441 cacctggatg gatttctata cctgtgaaaa atcttgaaat ttgagatatc gcgcgtgctg
    28501 caacatattt tttttggttg tataacttat gtgcaatcaa atgactccaa actgcatgta
    28561 aacctgcata cgttgtaatg acttctaatg ttgaacgtgc cgctggatcc tgctcaaata
    28621 ccatttttat atcgtctctc attcttttta acaagatcat ttcctcctca atgattgaac
    28681 tacgtaaata cataattgaa gtacctgcga aattaaatat caaaaaagca ccactaacat
    28741 acaagttgta ttgttagagg cgcttccgca cggttccact ctgaatttag cgaataacat
    28801 taataatatt gcgggcgctt ccaaattatc aaggaaacta agtcaactta atgctcatca
    28861 ctctcattat atatttaatt cattttacga aggtgcattc attaatttct acgttgtact
    28921 cacagcaacc gtacactctc tgcatcgtat aaatttaatt actaatcctt cgttttatat
    28981 aaaatacttt attcatattg ttgtaatttg ttaatacaac tttatcatat gtggttacgt
    29041 tataaaattc aagtatatac taccttgatc ttgtctattt cattacttat attgttttaa
    29101 acggtttagc actttttctt taccaagtac ttcaattgta tttggtaatt caggaccatg
    29161 catttggcct gttacagcaa cacgaatagg cataaataat tgcttgcctt ttattcctgt
    29221 ttctttttga acttctttaa ttgtcttttt aatttcagcc gcttcaaatg gttcaagtgc
    29281 ttctaattta ctgaataagt gcgtcattaa ctctggtact tgctctccat taatcacttg
    29341 ttgttcttct tcaccaagag ctggcatttc tttaaagaac atttctgata aaggtacaat
    29401 ttcaccggca taactcattt ctttttgata aagcgcaatt aatttgcgtc cccaagataa
    29461 atcctcttct gacggcacct caggaatcaa atttgcttta attaaatgag gtaatgctaa
    29521 ttggaatact gtttcagtat ctttttgttt catatattgg ttattaaccc atgctaattt
    29581 ttgcttatcg aaaaatgctg gtgattttga caaacgcttt tcatcaaaga ttttgataaa
    29641 ttcttcttta gaaaagattt cttcttcacc ttcaggagac caacctaata acgcaataaa
    29701 attaaataac gcttcaggta aataacctaa gtcacgatat tgctcaataa attgtaaaat
    29761 ttgcccatca cgtttactta actttttacg ttcttcatta acaattaatg acatatgacc
    29821 aaaacgaggt ggctcccagc caaatgcttc ataaatcata atttgtttag gcgtgtttga
    29881 aatatgatca tcaccacgaa ttacatctga aatttgcatg taatgatcat ctatagctac
    29941 tgcaaaattg tacgttggaa tgccatcttt ttttacgata acccagtcac caataccatt
    30001 tgaatcaaat gaaatatttc cttttaccat atcatcaaat gaatacgttt ggttttgagg
    30061 tactcggaaa cgaattgatg gttggcgtcc ttctgcttca aattgttgac gttgttcttc
    30121 agtcaaatgc gcatgttgac caccatagcg aggcatttca ccacgagcga tttgcgcttc
    30181 acgttcagct tctaattctt cttctgtcat atagcattta tatgctttat cttctgctag
    30241 taactgatct attaatggtt ggtagatatg ttgacgttca gattgacgat atggtccgta
    30301 gccattgtct ttatctacag actcatccca atctaatcct aaccatttaa gattatcaaa
    30361 ttgtgatgtt tctccatctt ctaaattacg ttttttatca gtatcttcaa ttcgaatcac
    30421 aaaatctccg ttgtaatgtt tagcatacaa gtaattgaat aatgctgttc ttgcattacc
    30481 aatatgaaga tacccagttg gacttggtgc atatcttact cttatacgat cgctcatttt
    30541 tttcactcct aaattaaata tcagattttc aagttagttc atataaattg ttcatttgct
    30601 atcttcgacc gtcataacaa atgtctaact cgtcttattg ttaaaacgaa acaatgcttt
    30661 ttaacatgac cttaaaataa tttcattgtt taatcataac ataattccct gggtaatatg
    30721 cttaaatttt aaatagaaag ctgttgtttt ttcaacactt taaaaaagct atccctaaga
    30781 atcaagactg cactacataa acatttcaac gacttaaaaa tgaatattat gtactgtgaa
    30841 attaaacttc aaattaacta ttcaaatacg ttaaaattga ttctaatttt gtatgtcttg
    30901 attgctataa gaataacttt attaatatct aaaatttaac acttaatgaa cttgtttcaa
    30961 tgatatatta gcactatttg tattttttga taactaatat gttttgcatt tatttatagt
    31021 tatacttcaa attacaaact tcgccatttc atataccttt taatatctat tttgttttcg
    31081 tcaactacag tttttataat gatactgtat cttcgatttt tttagcaaaa acaattcttc
    31141 ctgaagatgt ttgcaataag ctgactactt ctaaattgac atgactgcca ataagatttt
    31201 tagcattatc aacaactacc atcgtaccat catctagata tcctactgcc tgaccaggct
    31261 ccttacccat ttttgtcagt aaaatatgca gttgatcacc ttgatgtaca ttaggtttga
    31321 ttgcttctga taaatcatta acatttaatg ctttgatacc atgtacatga caaactttat
    31381 ttaggttgaa atctgtcgtt ataatacttg catgatattg ttttgctaat tttaataaca
    31441 tcgtatcaat atcactatgt gttttagttg gatgtataac ctttgtagga tagtctaaat
    31501 catacaattc atttaaaata tctaagcctc ttttaccctt ttcacgttta acactgtcat
    31561 ttgaatctgc aacaatttgt aattcattaa taacaccttg tggaattaaa atattgccat
    31621 cgataaaacc gcaacgaatg acttctaaaa tacgaccatc aataattgcg cttgtgtcga
    31681 taatttttgg cgtagcactt ttagtatgtt gtgacatgga acgcgctata ttctcaggta
    31741 aaaacattaa catttcatct cgttttttaa ggccaaattg gaaaccgaaa taacatagta
    31801 atatcgtaat tatgacagga atgaaatgat taaaaataga gttgccaatt gattctaata
    31861 taaacgacac cataacagaa ataagtaatc cgattattaa acctattgtt gcgaatagta
    31921 tttcaacagc acttctacgc ataataaaat gttctaaacc ttttatagcg ttagtaactc
    31981 gtctaataaa tacaccaaaa attaagaaca taaaaatact accgataatg ccatctacat
    32041 agtgattttt taaaaagctg gagttttgta atccaagatc atttgcaatt tcaggaataa
    32101 taattattcc taatgcgctc ccaataatta agtaaataat aataaccatt agtttaacga
    32161 tattcacaca atgtcctcct ttcttgatgt tttatgaatg aagagcaaat gacaatactt
    32221 catgtacagt agttacacct attacttgta taccttcagg atatgtccat ccgcctatat
    32281 tatttttagg aataattaca cgtttgaaac ctagttttgc agcctcttgc acgcgttgtt
    32341 ctatccgaga tacacgacgt acctcacccg ttaaaccaac ttctccaata tagcaatcta
    32401 atccgtcgac agctttatct ttaaagctag atgcagttgc tacaattaca cttaaatcaa
    32461 ctgctggctc cgttaacttt acaccgccag ctactttgat ataagcatct tgttgttgta
    32521 atagataatt ttctttcttt tccaaaacag ccatcaacaa acttaatcga ttatgatcaa
    32581 tccctgttgc cattcgtctc ggattgttaa aagtcgttgg agttaccagc gcttgaactt
    32641 ctattaaaag tggtctggtt ccctccatgg ttgcaacaat tgttgaacct ggaacatttg
    32701 ttgaacgttc ttctaaaaac atttcagatg gattatttac accttttaat ccactttgct
    32761 tcatttcgaa gattcccatt tcattcgttg aaccaaaacg gtttttaaca gctcgcaaaa
    32821 ttcgatatgc gtggtgttca tcgccttcaa aataaagcac agtatcaacc atgtgttcta
    32881 gcaatcttgg gccagcaatt tgaccttctt tcgttacatg acccactata aaagttgcaa
    32941 tgttcatttg tttagcaata ttcattaaac tttgtgtact ttcacgaact tgtgaaacag
    33001 aacctggcgc agagctgatt tcaggatgat atattgtttg aatcgaatcc actactaata
    33061 aatcaggttg ttcttctttt actgtttgat aaataacttc aagatctgtt tcagctaata
    33121 cttgcaattc acttgaatct tcatctaatc gctctgcacg taatttagtc tgactaagcg
    33181 attcttctcc agtaatatat agtacttttt tcttttgaga taacgatgca caaatttgta
    33241 aaagtaacgt tgacttacca atacctggat ccccaccaat aagtactaac gatccgctca
    33301 caatacctcc acctaataca cggttgaatt ctgctgaatc tgttaacact ctcggcgttg
    33361 tttcatgttt aatactattt aatttttgta ctttacctgc taattccttg gttttaactc
    33421 catgtttagg attggctgct ttttcaacaa tttcctccat ttgattccaa gcgccacaat
    33481 taggacattt ccccatccat ttaggagatt gataaccaca agccatacat tcaaaaatca
    33541 cttttttctt ggccaaaatt gcacctccac tttcctttga acaaatctat tttaaacgca
    33601 cattcccatt atgacaaatt aaatgtgagt tacatttgtt tgtattttaa catgactact
    33661 aacgcaaact gttttaccag atttattttt tatgaatatt ataaaataaa cttacatcat
    33721 gttgatagtc ggtatctttc aaagataaaa tcttaataat ttcttagtaa actcttttct
    33781 ctagatttat cacaatatta tatagaccta ttttattttg acgtaagttg ctagtatctt
    33841 caaacaaaaa acctttataa aaattcatac ctttatgcta tcgctgtagg ctcattaact
    33901 tgttacatat aattcttaac tatcctttga tgattgtttt attagattgt ttcgttgatg
    33961 gatactttca cgaatttcta tagttcaatg ctactaaaaa aacagcccaa aactttaatt
    34021 tgttttggac tgttttataa ttatgcttgc gatggtgttt tagtttctga agtttgttca
    34081 gcaatgtcat atttaaactc tttaccatca tgatctactg taactttctt accttcaatt
    34141 tgattaccat ctaatattaa ttcacttaaa ttatcttcga tagttttttg tatcgctcta
    34201 attaatggtc ttgcaccata ttctggatca tatccttctt ctgcgatttt gtctttcgct
    34261 ttatcagtta caataatatt tatgttttgt tcagataatc gatttgttaa tttattaacc
    34321 atcattgtta caatttcttt taattcttct tttgttagtt tatggaatac aatgatatca
    34381 tctacacggt ttaaaaattc tggacggaat gaatttttta attcttttaa catcgttttt
    34441 cgaattgttt cataatcttg tccatcactt gaaccaccga atccagcaaa tcgttgatct
    34501 tgtaattctt gtgccccaac gtttgatgtc attatgataa ttgtatttct gaaatcaact
    34561 gtacgtcctt ttgtatctgt caaatgtcca tcatctaaaa cttgtaatag aatattaaat
    34621 acatctggat gagctttttc aatttcatca aataaaatta cagaatatgg tttacgtcta
    34681 actttttcag ttaattgtcc accatcatca tgaccaacat atcctggagg agcaccaact
    34741 aatcggctca ctgcgtgttt ttccataaat tcactcatgt ctacacggat catcgcatca
    34801 tcatcgccaa acattgattc agctaaagct ctagctaatt cagttttacc aacaccagtt
    34861 ggtccaagga agataaagct accaattggt cgtttaggat cttttaaccc tgcacgggca
    34921 cgtctaaccg ctttactgat tgaattaaca gcatcttttt gcccaataac tctctcatgt
    34981 aatgtatctt ctagactaag aagtttttca gattctgttt cattgatttt agttaatggg
    35041 atacctgtcc atcctgcaat aacttcagca atatcttctt ctgacaatga agttgacatg
    35101 ccattttgtg cattcttcca ttcattttta gcttcttcat attgcttttc aagttttgtt
    35161 tgtttatcac gcaggttagc agcattttca aactcttgag catgtactgc ggcatctttt
    35221 tcatttttaa ctttttcaat ttcttgttca atttctttta aattattagg tgtcgtatga
    35281 ctcttaagtc ttactttaga acttgcttca tcaattaaat caattgcttt atctggtaag
    35341 aaacgatctg aaacgtatct gttacttaat ttaacagctg cttcaatagc ttcgtctgaa
    35401 atattaatac gatggtgtgc ttcgtaacga tctcttaatc cttttaaaat agcaactgta
    35461 tctactactg aaggttcatc aacttgtaca ggttggaaac gacgttctaa agccgcgtct
    35521 ttttcaatat ttttgcgata ttcatctaat gtagtagcac caatacattg taattcacca
    35581 cgtgctaatg ccggcttcaa aatattcgaa gcatcgatag caccttcagc accaccagca
    35641 ccaactaaag tatgcaactc atcaataaat aggatgacat tacctgcttg ttggatttct
    35701 tccataacct ttttcagacg ctcttcaaat tcaccacgat atttagtacc tgcaactact
    35761 gttcccatat ctaaagacat aacacgctta tcttttaatg tctctggtac ctcattattc
    35821 actatggctt gcgctaaacc ttcagcaata gcagttttac caacacctgg ctctccaata
    35881 agcacaggat tgtttttcgt acgtctactt aatacttcaa ttacacgtgt aatttcttta
    35941 tcacgtccta taacaggatc taatgtaccg tctttggcaa tgactgttaa gtcacgagct
    36001 aaactatcta aagttggagt attatttgac ttactagctt gtgcattttt attactcatt
    36061 tcagggtttc ctaaagcttt cacaacttgt gcacgtgctt tagtaatatt taaatctaga
    36121 tttgcaaaaa ctcttgctgc aacaccttca ttttcacgaa tcaagcctaa taaaatatgt
    36181 tccgttccaa caaaattgtg atgtaatttt ctagcttcat ccatcgataa ttcaatgact
    36241 tttttagctc taggtgtata atgcaatgta ccaacatgat cttgaccatg tccgattaat
    36301 ttttcaactt cttcaattac tttatcttca gtgatattaa aactttctaa tacttttgca
    36361 gcaattcctt caggttcttt cattaacccc aataataggt gttctgttcc tatatttgaa
    36421 tgatttaaac gaattgcttc ttcttgggca tgtgctaata cgcgctgtgc acgctcagtt
    36481 aatctaccaa ataacataaa taatgacctc ctactttata tgttctctta gtatatctgc
    36541 tcgtttttct tttacagatt tgtcatcttc ttcatctaat aaaaatggtg actgtatagc
    36601 taccatcaat tcattaaatt taaagttttg taattcaatg taatttaaat ctataccaag
    36661 tttaacttcg cttaatctat aagaagcctc ttccatagtt atcattctac agttttgtaa
    36721 aatacctagc gagcgaaaaa cacggtcttg tgtttctaat tgattataag tgtctaactt
    36781 ttgtcgtatt tgtttttctt catgaatgat ttgattaaca acttctgtta atgtttctat
    36841 gatttctaac tcagatttac caagtgtaag ttggttggat acttgataag tatgtccata
    36901 aacttgcgaa ccttcaccgt aaatacctct gattgtatat ccaaaacgat taatggtttg
    36961 agcaatccgt gtcattcttt tcataataga tagacctggc aaatgtagca tcacgcttgc
    37021 tctcatacca gtacctatat tggtaggaca tgtagttaaa taaccaagtt gttcatcata
    37081 acttatatca aggcttcgat ctaattcatc atcaattgat gaagcttgat tatataaagc
    37141 ctgtaatgtc gtgtcagttc ccatagcttg aatacgaata tggtcctctt catttatcat
    37201 gacacttaaa gattcatcgt cattcactaa tactgcggct gctggttgtt ttattagttc
    37261 aggactaatc aaatgctttg caaccatttt cattttactt tgttgatcca tttgatcaag
    37321 acgcattaat tcaaagtttg gcaaggcatc ttgtacctca tttataactc taaatccatc
    37381 attttcagta gcatacatta gtggatgcac atgattttct aaattacgcg ctaaccgaat
    37441 tctagaagac ataacaattg gtgtttcttc attacttttc atccattggc tgatattatc
    37501 atgaatatta tgcgtcatca tgttgcacct cactctcagc ttttagtgct ttaatttcat
    37561 ctctaacaat ggctgcttcc tcaaaatctt ggatttcaat aagttttttc aaatattcat
    37621 tcttttcttc gatttttcgc tttaaagcta tctttttatg tgaagaatgt ggtgtctttc
    37681 caacgtgctc aaattgtcca ccttgaactc tgcggacgat atcaatgatg tcatctttaa
    37741 atgttgcata acaattagca cacccaaatt taccaacatg tgcaatatct ttcaaagtca
    37801 tatgacatga tgggcaacgt ttttcttctt taaacgctac ttcttgaaaa ttaattccat
    37861 gtttcgtagc taaatgttgt aaaatttgct taacaacaaa tgcttcttcg aaattatctt
    37921 gatgttcttg atattcaggt tgttcattag cttgattcca cggatggtgt ccctcagcac
    37981 aagtttgaca caccattttt tcttctgttt tatttttact tgtaacttta acttttaatt
    38041 ccgcttcatt aagttgacaa ttttcacaaa gcacttcaac acctcatttc atttagtaat
    38101 aatttataac tggtaacaaa cgttttaaaa tatttgctct aataatatct ctagaaacca
    38161 tatccattga tagcgtttct ctatcaataa ctgcttgaat cattttagct tcacgttcat
    38221 ttattaacat tttatctaaa agcccatcaa taatataata agcttgttgt tgagaaatag
    38281 aaggtccaat cagctgaagc aaatgattaa tataacctgt tgcatcttta ttttcaattt
    38341 tagtgattcg gatgtaacca ccaccaccac gtttactttc gatttcataa ccatgttcat
    38401 tagtgaatcg tgttttgatt acataattta attgtgatgg tacgcaatca aaacgctgtg
    38461 cgatattcgc tctctgaatt tcaacgacat cttcattcga ctcttcaaat aaacgtttga
    38521 tgtattgttc tatgatgtca gacatattgt gcatgtatat cacccctttt tgaccttctt
    38581 tgactatatt atatgaccta ttttgacctt tttcaattaa tttgttttta tattttcaaa
    38641 aatttatgat gttagccctt ccattttatt gtatgataga ttgtagaaaa aactaaagga
    38701 atgagaaata tatgcatatt ctaattggga ttatcggaat tatcttcttt ttagcacttg
    38761 cgtttctatt tagctcagac aggaaaaata ttcgctggaa atatgtagga attttacttg
    38821 caattcagct tatctttgca tttatcttgc ttaaaactac aatgggaatc aaggttattg
    38881 gtggtatttc aaacggtttt aactacttat tattaaaagc agcagatggt gttaactttg
    38941 tattcggtgg tattcaatat atcgatccta agcaaccacc attcttcttc agtgtattat
    39001 tacctatcgt atttatttca gcgattatcg gtatccttca atacacaaag attttacctt
    39061 taatcattaa tgttttagga ttcttaattt ctaaaattaa tggtatggga cgtttagaat
    39121 catacaatgc agttgctgca gcaattttgg gacaatctga agtatttatt tcaattaaaa
    39181 aacaattacc atacatacct aagcaacgtt tatacacatt aacagcttca gcaatgtcga
    39241 ctgtttctgc ttcaattatc ggtgcatact ttaccttaat tgaacctaaa tatgttgtaa
    39301 cggctgttgt cttaaactta tttggtggtt tcatcatcgc ttcaattatt aacccttata
    39361 aagttaatga agaagatgac aaattattag ttgaagaaac tgaagaaaga caacaatcat
    39421 tcttcgaaat gctaggagaa tatattttag atggatttaa agtagctgtc attgtaggcg
    39481 caatgttaat cggatacatt gccattatcg ctctattaaa tggaattgta agcaatatct
    39541 ttagtacagt ttcaggtggc gctatttctt gggatttcca aacattaatt ggttttgtct
    39601 ttgcaccatt tgcattctta gtcggtgtgc catggcaaga tgcagttcaa gccggttcag
    39661 ttatggcgac aaaactatta tctaacgaat ttgtagcaat gcaagcactt ggtaaactta
    39721 gtgatttatc tgaacacgca aaaggtgtta catctgtatt cttagtatca tttgctaact
    39781 ttagctctat cggtattatt tcaggtgcga ttaaatcact taatgacaaa aaaggagata
    39841 cagttgcccg ctttggtttg aaattattat tcggtgcaac actagtatct ttcatttcag
    39901 ctgcaatcgc tggattcttt atttaacaaa tacatcaaca tcgaattaac cccttcaaaa
    39961 ttttgactat tctcagtctc tttgaagggt ttttttctta tgtatagctt tattttttat
    40021 catttttaat tttgacataa aaaataactc ctccaaagct aagtttgaag gagatatttt
    40081 cttctcaaca aatctataaa tatcaataat tcgttgattt agcatacatt aagctatgct
    40141 ttttttacaa tatgattaat aaagtaatca gttactctat agtcatctgt taattcagga
    40201 tggaatgata cgcctaaata tttaccttgc tgaacagcta caattttctc attaacctta
    40261 cataggatat ctacgccttg acctactttt tcaatatgtg gggctcttat aaagacacct
    40321 tcaatatctg tagcgatacc tttaatatct aattctgttt caaagctgtc aacttgtcta
    40381 ccgaatgagt ttcgttgtac agtaatattc aacttgttaa ggtatccttc ttcaccaact
    40441 atatcttgcg ctagaactat taatcctgcg catgtaccaa acataggtaa agttgaattt
    40501 tgtaaagcct ctttaaatcc atataaattc attaatcgac gtaacgttgt agactcgcca
    40561 ccaggtaata ttaagccctc gatttcttct aattgttcaa cttttttaac tgcaatacct
    40621 tcatgaccac ttaattcaat atgtctaata tgttcacgta ctgcaccttg taatgctaat
    40681 acacctattt tcatatctta ccaaccacgc tcttgcatac gttcttctaa tgataattga
    40741 ttgatatcta aacctttcat agcagtgcca agttcacttg ctaatcttcc aattagttca
    40801 tagtcttggt aatgtgttgt tgcttgaaca attgctttag caaatttttc tggatcttct
    40861 gatttaaaaa tacctgatcc aacgaataca ccgtcagcac ctaattccat cattaaagca
    40921 gcatcttgag gagtcgcaac gccaccagct gcaaagttaa ctaccggtaa acgaccattg
    40981 tctttaattt gttttaaaat ttcataaggc gcaccgatat ctttcgcaaa agtcataatc
    41041 tcatcatcat tcattacagt caatcgacta acttctgaat taacttgtct catatgtctt
    41101 acagcttcaa caatattacc tgtacctggt tcacctttag tacgtaacat agcagcacct
    41161 tcaccaattc tacgcgcagc ttcacctaaa ttacgacatc cacatacaaa tggtactgta
    41221 aattgatctt ttcttaagtg atattcctca tctgctggtg ttaacacttc tgattcatca
    41281 atatagtcaa cacccatcgc ctctaatact cttgcttcag tgatatgacc aatacgtgct
    41341 ttagccatga ctggaataga aacagcattc attacttctt ctacaatttt agggtttgcc
    41401 atacgtgcaa caccaccagc agctctaata tcagaaggta ctcgttctaa tgccataact
    41461 gctaccgcgc cagcttcttc tgcaattctt gcttgctcag cattaacgac atccataata
    41521 acgccgcctt tttgcatttc agccatacct cttttgactc tgtctgatcc aataatttta
    41581 ctcattatac ataaaacccc ttttcaagtt gattaaatac attgtaatgt ataaactgat
    41641 atttaaaaag gtacaaaata agataaattt aggaggtcag ttataaaatt gaaaaacact
    41701 ttataccatc aactttatga aaaattaaaa aaacaaataa ttgaaggtca atttaaagaa
    41761 ggcgataaat tttattctaa aagacaatta agcaaacatt tatctattag ccaaacaacc
    41821 gtagagcatg catatcaact tctattagac gaaggttaca tttattcaag acctcgctct
    41881 ggatattttg tgtccgaaat tgaatcttta acaattttaa acaatcaacc tatcccctct
    41941 ctttttgatg atgattcata taaaccaaaa gcgagtgatg aagcttatga ttatgctttt
    42001 aatttggatg aaatagatac gaaacatttc cctatagaac ttttcagaaa gtattccaaa
    42061 gatctttatg atacaaatca tttaaatcaa ttacgtagag gacattttca aggagaatta
    42121 catttgcggt ttcaactggc attttattta tttactaatc gtggcgtaat ttgtgatcct
    42181 aatcaaatta taattggctc atctaccgaa caattagtaa atcaattagt cgatttactt
    42241 tatacttcaa catttattat cgaaaaacct agttatccac caatcaaaaa tattttagat
    42301 aaaaaacaag ttgaatatga acaaattgaa gttgaagata atggaataaa cgtagatgaa
    42361 gttattaaat cacaaaaaaa tatagtatat ataacgcctt cccatcaatt tccaactgga
    42421 tatgtgatgg acttaaaaaa acgaactcaa ttaatacaat gggctcaaga aaaagaagaa
    42481 cgatttatta tagaagatga ttatgattcc gagtttagat atttcggcaa acccattcca
    42541 gcaattcaag gtctatattc aagaggagaa aaagtgattt atatcagtac attttcaaag
    42601 tctatttttc ctagctgtcg agttgcatat atggtgttac cctattctat aatgaaaaaa
    42661 tatcattctc aaaatcacat tgaaggcaac actgtacctg ttcatatgca aaaccttatt
    42721 gcaacgttta tatcttccgg tggttttgaa aggcatttaa ataaaatgag acgaatttat
    42781 cgtagaaaac taacctatat tttaaaaaga ttgaaacctt acaaagagca gcttgatatt
    42841 caaggtgctg aaacgggtat gcattttaca attacagtaa aaaatggatt aacattacaa
    42901 gagtgtcttg atagagcaaa caaagtaaag ttaaaattac aagtttataa ttttgatgat
    42961 gatcaagact ataagaaaac ccctaaattt atattaggct ttggcggtat agacgatgat
    43021 gctttaaaac cgcatgtaga cgctctaata aaatcattaa ttataaagta acacttcttt
    43081 ga
//
LOCUS       DAKTLH010000008        41468 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.008, whole genome shotgun sequence.
ACCESSION   DAKTLH010000008 DAKTLH010000000
VERSION     DAKTLH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 41468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41468
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.008"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(443..541)
                     /locus_tag="O0394_000335"
     CDS             complement(443..541)
                     /locus_tag="O0394_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498451.1"
                     /translation="MNWIIELDLWLWCVGAINLINAVNDYANEILL"
     gene            complement(746..1342)
                     /gene="recR"
                     /locus_tag="O0394_000336"
     CDS             complement(746..1342)
                     /gene="recR"
                     /locus_tag="O0394_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCY0498452.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            complement(1349..1666)
                     /locus_tag="O0394_000337"
     CDS             complement(1349..1666)
                     /locus_tag="O0394_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCY0498453.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            complement(1756..3453)
                     /gene="dnaX"
                     /locus_tag="O0394_000338"
     CDS             complement(1756..3453)
                     /gene="dnaX"
                     /locus_tag="O0394_000338"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCY0498454.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPVQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            complement(3522..4046)
                     /locus_tag="O0394_000339"
     CDS             complement(3522..4046)
                     /locus_tag="O0394_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0498455.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            complement(4184..4285)
                     /locus_tag="O0394_000340"
     CDS             complement(4184..4285)
                     /locus_tag="O0394_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498456.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSELDVQFE"
     gene            complement(4354..4623)
                     /gene="ffs"
                     /locus_tag="O0394_000341"
     ncRNA           complement(4354..4623)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="O0394_000341"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4685..5413)
                     /gene="treR"
                     /locus_tag="O0394_000342"
     CDS             complement(4685..5413)
                     /gene="treR"
                     /locus_tag="O0394_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCY0498457.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRIK"
     gene            complement(5438..7078)
                     /gene="treC"
                     /locus_tag="O0394_000343"
     CDS             complement(5438..7078)
                     /gene="treC"
                     /locus_tag="O0394_000343"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCY0498458.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWYSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTIGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLAREGCVVIQTGTVENNTISGFGAIVIETNA"
     gene            complement(7142..8569)
                     /gene="treP"
                     /locus_tag="O0394_000344"
     CDS             complement(7142..8569)
                     /gene="treP"
                     /locus_tag="O0394_000344"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCY0498459.1"
                     /translation="MAVKREDVKAIVTAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
     gene            complement(9040..9132)
                     /locus_tag="O0394_000345"
     tRNA            complement(9040..9132)
                     /locus_tag="O0394_000345"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9094..9096),aa:Ser,seq:tga)
     gene            complement(9401..10864)
                     /locus_tag="O0394_000346"
     CDS             complement(9401..10864)
                     /locus_tag="O0394_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HCY0498460.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLAAAEELNLLGYQVTIYERARESGGLLMCGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            complement(10882..15381)
                     /gene="gltB"
                     /locus_tag="O0394_000347"
     CDS             complement(10882..15381)
                     /gene="gltB"
                     /locus_tag="O0394_000347"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCY0498461.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEVVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKR
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGDLEDALEALGREAVNAVKQGAQILVLDDSGLVDSNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATSGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            15562..16446
                     /locus_tag="O0394_000348"
     CDS             15562..16446
                     /locus_tag="O0394_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0498462.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPSLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
     gene            16561..17679
                     /locus_tag="O0394_000349"
     CDS             16561..17679
                     /locus_tag="O0394_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCY0498463.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMADGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            17676..18458
                     /locus_tag="O0394_000350"
     CDS             17676..18458
                     /locus_tag="O0394_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCY0498464.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(18627..19112)
                     /locus_tag="O0394_000351"
     CDS             complement(18627..19112)
                     /locus_tag="O0394_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0498465.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFVGRPVHIMALTI
                     K"
     gene            complement(19102..19497)
                     /locus_tag="O0394_000352"
     CDS             complement(19102..19497)
                     /locus_tag="O0394_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCY0498466.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESQVHALL
                     REVKEELNLTLTMDEIEYVGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            19646..19915
                     /locus_tag="O0394_000353"
     CDS             19646..19915
                     /locus_tag="O0394_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498467.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            complement(20100..21104)
                     /gene="aaa"
                     /locus_tag="O0394_000354"
     CDS             complement(20100..21104)
                     /gene="aaa"
                     /locus_tag="O0394_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HCY0498468.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRG
                     YNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(21436..22278)
                     /gene="gmpC"
                     /locus_tag="O0394_000355"
     CDS             complement(21436..22278)
                     /gene="gmpC"
                     /locus_tag="O0394_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HCY0498469.1"
                     /translation="MKRLIGLVIVALVLLAACGGNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            complement(22315..22974)
                     /locus_tag="O0394_000356"
     CDS             complement(22315..22974)
                     /locus_tag="O0394_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0498470.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            complement(22978..24003)
                     /locus_tag="O0394_000357"
     CDS             complement(22978..24003)
                     /locus_tag="O0394_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCY0498471.1"
                     /translation="MIEFRQVSKTFNKKKQKIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGK
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGDFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFEDTAINQYFKEKN
                     IQFEEVH"
     gene            complement(24297..25439)
                     /locus_tag="O0394_000358"
     CDS             complement(24297..25439)
                     /locus_tag="O0394_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCY0498472.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIATLENGKHGLAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            complement(25423..26337)
                     /locus_tag="O0394_000359"
     CDS             complement(25423..26337)
                     /locus_tag="O0394_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCY0498473.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGAEVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKQIFNYEENNNEQEN"
     gene            complement(26554..27891)
                     /locus_tag="O0394_000360"
     CDS             complement(26554..27891)
                     /locus_tag="O0394_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HCY0498474.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVIGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIVIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            complement(28056..28169)
                     /locus_tag="O0394_000361"
     CDS             complement(28056..28169)
                     /locus_tag="O0394_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498475.1"
                     /translation="MGIAPEVVMWRDVGDIDGVVCGEILAHVYSVEPENLM"
     gene            complement(28347..29081)
                     /locus_tag="O0394_000362"
     CDS             complement(28347..29081)
                     /locus_tag="O0394_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCY0498476.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            complement(29118..29792)
                     /locus_tag="O0394_000363"
     CDS             complement(29118..29792)
                     /locus_tag="O0394_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCY0498477.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISLA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            29830..30246
                     /locus_tag="O0394_000364"
     CDS             29830..30246
                     /locus_tag="O0394_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498478.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVRRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            complement(30475..30837)
                     /locus_tag="O0394_000365"
     CDS             complement(30475..30837)
                     /locus_tag="O0394_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCY0498479.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(30999..33704)
                     /locus_tag="O0394_000366"
     CDS             complement(30999..33704)
                     /locus_tag="O0394_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HCY0498480.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPEMTIESVLTHFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHYFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVAKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQP
                     HSVLLNDTQAVDENNSELNQMGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAVAEHHTSTDTLAWVYVPDT
                     LSSIALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGTLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVIQAPDYVVARLLANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            complement(33717..35201)
                     /locus_tag="O0394_000367"
     CDS             complement(33717..35201)
                     /locus_tag="O0394_000367"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HCY0498481.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGITLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHIVHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILGWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYVITHNYFYAVLQNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKAFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            35722..35787
                     /locus_tag="O0394_000368"
     CDS             35722..35787
                     /locus_tag="O0394_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HCY0498482.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            35819..35884
                     /locus_tag="O0394_000369"
     CDS             35819..35884
                     /locus_tag="O0394_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HCY0498483.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            35936..36004
                     /locus_tag="O0394_000370"
     CDS             35936..36004
                     /locus_tag="O0394_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HCY0498484.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            36068..36130
                     /locus_tag="O0394_000371"
     CDS             36068..36130
                     /locus_tag="O0394_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HCY0498485.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(36789..37991)
                     /locus_tag="O0394_000372"
     CDS             complement(36789..37991)
                     /locus_tag="O0394_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCY0498486.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKMLSALQPTAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTELDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            38110..38370
                     /locus_tag="O0394_000373"
     CDS             38110..38370
                     /locus_tag="O0394_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498487.1"
                     /translation="MSALLYNGDKDISHERESRCINMLFVILVLYVTGIAFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            complement(38660..38974)
                     /locus_tag="O0394_000374"
     CDS             complement(38660..38974)
                     /locus_tag="O0394_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498488.1"
                     /translation="MTLIEPDMTLRMPDISTTVETLNLISKMEAQKENIRTVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(38993..39316)
                     /locus_tag="O0394_000375"
     CDS             complement(38993..39316)
                     /locus_tag="O0394_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498489.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            complement(39313..40641)
                     /locus_tag="O0394_000376"
     CDS             complement(39313..40641)
                     /locus_tag="O0394_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498490.1"
                     /translation="MLSRKYKIDLKAINQNTESTSAISKASYEVENANNNGLSKRDVI
                     NQFNDLKKMKKFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNLQDEAFKK
                     LKEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFI
                     TGHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITR
                     FVAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYA
                     EENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQ
                     LIDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMV
                     REPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            complement(40631..41443)
                     /gene="lpl10"
                     /locus_tag="O0394_000377"
     CDS             complement(40631..41443)
                     /gene="lpl10"
                     /locus_tag="O0394_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl10"
                     /protein_id="HCY0498491.1"
                     /translation="MEYIKKIALYMSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEE
                     QIKKSFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTILTGFSKSNKPGVLDDE
                     GMVLYLNRNAKKATGYYFVNKVYDDISKNHNEKKYRVELKNNKIVLLDNVEDKKLKQK
                     IENFKFFSQYADFKDLKNYQDGNITTNENVPSYEAQYKMNNSDKNVKKLREIYPITTN
                     NSPNLKLYIDGDIKGSSVGYKKIEYKFSKDKGQETTLRDYLNFGPSEGENVE"
BASE COUNT        12971 a         8508 c         5965 g        14024 t
ORIGIN      
        1 tttcttaccg acaagaatta attatacatt ttattaacat ttaagtcaat aactttttaa
       61 aattaatttt cgttagtaat tcaagttatt tcttaagttg agttgttttg tttgcctcaa
      121 caaataaata ctatacaggc tttcaaactg cttaacaaca gtaaaaatta cacctttgtc
      181 atctgtactg aacgccctta tcctaaaaat cgaacttaaa aaatccccac ttataaatag
      241 tacgtttttt agaacaagaa acagattttt ctctaaccat atcaatcaaa aaaaacgcaa
      301 aacagaacgt tattttttta ccagaaatta aaaataaatt tacaagtttt aaataatctc
      361 tcaaaacacc gctatacagg ctgatttaat aagctttaga ccaaagaagt acaacttact
      421 aagccagcat ctatcatttc aattacaaaa gaatttcatt tgcatagtca ttaaccgcat
      481 ttatcaaatt aatagcacca acacaccata accatagatc taattcgata atccaattca
      541 catcattact attagaagta acaaatcaca accaacggct tgcttcctaa agattaccca
      601 tccctcagta aaactaggta aaccatacca ccgccacacc gcaaataaag tagtacatca
      661 caccacacca taaaaaaagc cacaatgatt acactgtgac ttttcttact atagtattaa
      721 aatcaaaaat gtttaataga agacattaca tttctgttct acctgcgatt gctttagata
      781 atgttacttc gtcagcatac tctaaatcgc cacctaccga taacccttgt gctaatctcg
      841 tcactttgat acctataggc ttaactaatc tagaaatata catggctgta gattccccct
      901 ctaagttcgg gttcatagct aagattaatt cgctaacttc atcgtttttc aagcgttcaa
      961 tcaatgaagg aatattaata tcttctggtc caatgccatc cataggcgaa atagacccat
     1021 gtaaaacgtg atataaacct ttgtattctc tcattttttc catagctatg acatctttgt
     1081 catcttccac aacacaaata actgaacgat ctctttgctt atcttcacaa atataacatg
     1141 gatcattttc agtaatgtga ccacatacgc tacaatatgt taattctctc ttaacatcta
     1201 ctaatgcttt ggcaaactga acaacatcgt cttctttcat atctaaggta tgaaaagcca
     1261 gacgttgggc tgtctttgga ccaatgcctg gcaatttcat aaagctatca ataagttttg
     1321 atataggttc tggataatgc atctatgatc acattccagg gatgtttaag ccttgagtat
     1381 gtttacctaa acgttcttga gtaagctcat cagctttatt catcgcttca ttagtagctg
     1441 ctaacactaa gtcttgtagc atttcaatat cgtctgggtc tacagcttct tctttgattt
     1501 caacgtcgac aacttcttta tgaccagtta cagtaactgc aaccatgcca ccgccagctg
     1561 ttcctacaat acgctcttct ttaagttttt cttgttcttg agccattttc ttttgcattt
     1621 tttgcatttg tttcatcatt tgttgcatgt ttccgccacc gcgcatattc catttcctcc
     1681 ttgaaaatca ataaatattt atcaataaaa tgatgtttct ttcattatac atacgattat
     1741 atcgcttgtc atgtatcact cttcatctat cacatgtaca gtttcttcac cgaaaagatc
     1801 ttttgctttt tgagcaatat ctgtttgttg tgcttgttgc tttggcatat catcgccttc
     1861 gtttttacga ttttgtaaat attccgttcg aactctttgc cattgatctg atggtacacc
     1921 aacaacttta acgtttttat taacgatatt acatacaaca ctttctatac tactacgttt
     1981 ctcgtcgtct ttattgacga tttcacaatg gatctcttcc tcaaatttca caagtacgtg
     2041 atcttcactt gccgccacag gttccgaatt ttgcaataaa ctaacgagtg attttttatc
     2101 attatttttg gcatgatcaa tcacttcttg ccaatgatct ttcaacaatt tgatatctgc
     2161 cttattcgct ttatctagca cttttgcaat ttgttgcatt gaaaatgcat ttttagattt
     2221 ttgtatgcct ctcgcaggct ttttcgaaga tttttgaaca ggagcgacac tcactccttg
     2281 tgcttttagt gtttttagtt cttgctctaa ctgttccata cgttgcaaca atacatctgt
     2341 gtttggcgat gaagcaattt gtgctggttc agctacattc gcaatcactt gtggttgacc
     2401 cttaatctgc tcagctaatt ttactaacaa cacttcaaaa tgaacgtttt gattcacact
     2461 aaaacgaatc gacactaatg tatcattaat aagatcaatc atttgatata acatatctaa
     2521 ttctaagttc atcagtgctc gatactcagt atctttctca gatgttttat tcataatcgt
     2581 atctctgaca aaataaatca tatcatttat taggcgattc acttctttac cttctgttat
     2641 aaactgatgg tattttttaa aagatgcttg tacgtcacct tgtacaatat catcaaacaa
     2701 gtgatccaac gcttcatcat gtacgctacc tgtgacattc aacgcatctt gcaatgttaa
     2761 cgtaccatca ccaaatgcaa tagcctgatc cataatactt aatgcatcac gcataccccc
     2821 ttcagacgct ttagcgataa atgccaaggc ttcatcttca cattcaattt gttgtgcatc
     2881 tgctacaaat tttaaacgtt caacaatttg atctaggcta attgctttaa aatcaaaacg
     2941 ttgtgcccta gaaatgattg ttggagggat tttatgtggt tctgtcgttg ccaatataaa
     3001 aatagcgtgt gctggaggtt cttctaacgt ctttaaaagg gcattaaaag cacctgttgt
     3061 tagcatgtgc acctcatcta taatataaac tttatatttc gattcacttg gtgcatattt
     3121 aactttgtct ctaatatttc ttatttcatc aaccccatta ttactagcag catcaatttc
     3181 tatcacatct gaattagtcc cctgcgtaat gcctttacaa atatgacatt cattacaagg
     3241 ttctccatca gtgctattta gacagttgat tgctttagca aacactttgg caatactcgt
     3301 tttccccgta cctctcggac cactaaaaat ataagcatgc gactgttttt ctttcgaaat
     3361 cgcattgcgc aatgtcttcg tgacatgttc ttgtccgacg acatcctcga aactttgggg
     3421 tctgtacata cgatataagg cttgataatt caagttagca cctccataaa caattacctc
     3481 tcattatagc atgataatac ctttacttct taattgaaca attaataaaa atgttctgga
     3541 aattttacga ttccgtgtgg tgcatccgtc aaattatccc ataaatattt tacaagtaac
     3601 ggtgcatcac cagattctaa tttaatgtca tgctcagcag cattttgata acccaacttt
     3661 tcaaagtagt caaaacaatg gtctacaaca accgtactat actcttgtgc tttggcacgc
     3721 tcttctactg cttgaaccaa gccacgacct aatttttgtc cacgtaattc aggatgaact
     3781 gataaagagg caatcgccaa accataatac gtcttatcat cactattaat ttctacttca
     3841 attaataaaa cgtgtccaac gacatcgtta ttttcatttt tcgctattac ttctaattca
     3901 aaattatagc aaggagattt tcttaaatgt tttactttcg cacgtgcttg ccaactcgtt
     3961 tcaggattat catcaaaact ttcttcaata ctatttaaag atttatcata atctaactct
     4021 gttaaagtac ttaaatatat ttgcatatgt cctccgtagg catttgattg tcaataatca
     4081 taacgtatct cattaataat tctattgtaa gatactcccc ctcatttcac catcatttct
     4141 ttatcatcaa attataactt cttacttttc attgacacaa aaatcattca aactgcacat
     4201 cgagttcact tgaatcaaac ttcacatata aaaaagctac ttcccacaaa catgttccac
     4261 gtataatacg ctgaattgtc ttcaagaaag tagcttctat aattatatat tttcaactct
     4321 taataatcgg tttaatattt aaaaaataaa aaaccgtgca cctaagcatc gaacgtaatt
     4381 cacgaacgta accaaagtcg ttagctaaat ctcggctacc ctacggcaca tatgatgatc
     4441 cacttaatgc tgcttccgtc aggacctgac atggttcatg ggttcatatt gcataggacc
     4501 gaaatcttca aacactacgt gctttgggca gacttcgcaa aaatacggcc tcaacaaagg
     4561 aattaagcct cgcataaagc ggatttcgag tacagggaac cgctacctcc ccacctagca
     4621 cggcaagata tatattacta tattttaata gttaattgca agtataaatc atttatatca
     4681 ttgtttactt tatacgacgt cttgagaagt cattaaattt aaattcattt gcaagatgtt
     4741 ttgaaatatt atattgaaac ggcattgtat tttctaaata cacaatactt cgaactgttg
     4801 ctgaataagc caccgataca tcaccaaaca attgatatgc ttgttcatca aacggttcaa
     4861 aagtaataga cttacttgaa taactaatat taagatttaa taccttttcc aaataatcat
     4921 aaatagaatc actcgcaaca tcattaccta tatctgaaac aatcgacttt agaaaataat
     4981 cttcatcaac aatcttcaca tgttggttaa gccgacgagt tctaacaata tgaatgagtg
     5041 attcactaga attgatgttt aaagcatgtt gaacttctgg aacttcatgc gcttcaacaa
     5101 cctcattcac aacaacttca gttaaatatg cgacgcccat ttcttcttgc atttctttaa
     5161 aactaacaag ttcagaaaat ggaaactctg taacctcctg ataaatgaca agtgaccctt
     5221 taccatgaat cttttgaatc atgccgtcta atgccaacaa atctaatgcc ttacgcacgg
     5281 tctctcgaga tgactggtac aattgcacca aatcatgttc agacggaatt tgttcaccat
     5341 attgaatctg cccgtttaat atatcttctt tcaacgcctc ataaatcttc ataaactttt
     5401 tttgtttcgc catataaacg catccactca atttatttta cgcgtttgtt tcgattacaa
     5461 ttgcaccaaa cccgcttatc gtattatttt ccactgtgcc tgtttgaatc acaacacaac
     5521 cttctctagc caatccttct ggcaaatcaa cagccgatgc tgagaaattc gcaattacta
     5581 gccattgttg attcttataa tgacgttcat aaacaaataa atgatcatga tccataaaac
     5641 gtggcacaat gtctccatac gtaataatat catgcgtatg tcttaattgt attaattttt
     5701 gatacgtata gaaaatagac tctttattct gaagtgcttg tctaacgttg acctgatgat
     5761 aattttccga aatatcaatc caaggcttac cagctgtaaa tccagcatta acatcatcac
     5821 tccattgcat aggcgttctc gaattgtctc gtgacttctg tcctaaaatc gctaacacat
     5881 ccgcttcagc atgcccttca cttaacaact gatggtaggc attaatcgat tcaacatcac
     5941 gatattgtgc tattgatgta aaatgtgggt ccgtcatacc aatttcttca ccttggtaaa
     6001 tatatggcgt cccttgcaac atatgcagtg cgatagctaa cattttagca ctttgtatcc
     6061 tcatctcttc cgacgtatca tcaccaaatc tagacactac ccgtggctga tcatgattac
     6121 accagaaaat cgcgttccat ccgccaccgt cataaatacc tcgttgccat tgcatcagaa
     6181 tttcctttaa cttatgaaaa tcaagcttcg catttgtcca cttttcacca tcaacataat
     6241 caacctttag atgatgaaaa ttaaaaacac tattcaattc ttggcgttct ggttgtgtat
     6301 acttaataca attttcaatc gtcgtcgaag acatttctcc tatagtcatc atgtcagtgt
     6361 taccaaacgt ttgacgattt aattcatgca gaaactcatg cactctagga ccatccgtat
     6421 aaaattcttt acctattttg tcagagtcct taaattcacc tttagaaatt aagttaatga
     6481 catcaaatcg aaaaccatca acgccgaagt ctatccaatg attgactatg cgatataacg
     6541 attgacgtac ttccggatta tcccaattta aatcagcttg actgacatca aataaatgta
     6601 aataatattc atctgtctca gaatcatact tccatgcatt accaccgaat ttagaatacc
     6661 aatttgttgg cggcccgtct tcagatgatc tgaaaaagta ataatctcta taagggttat
     6721 ctttagattt acgggcttct ttaaaccatt catgctccgt cgacgtatga ttaatgacaa
     6781 tatctaacat cactttcaag tctttttgat gcgcaacttt gattaacttt tcaaaatcat
     6841 ccatcgttcc aaagtcttca ttgatttcta aataattgct gatatcatag ccattatcat
     6901 tcatcggtga ttcatacact ggtgttaacc aaatataatc aacacccaat aacttgatat
     6961 aatccaattt ttctataatt ccattgatat ctcctatacc attccccgtc gtatcattaa
     7021 acgacttagg ataaatttga tatacaacgg attttctcca atctatttct ttcgacacac
     7081 aattaccacc ttataacgta ataaaggacg tccaaataac gaacgcccct tttttatttt
     7141 attaatcttc aacaatttct ttcgcttttt gtttactaaa atgagacatc acaattgtta
     7201 gtatacatgg tacaacaata gcaatagctg tcacaataag atatactggc caaaattctt
     7261 tttgaattga aatgaatgct ggcacgccac caacaccaac ttttccaagt acgttattca
     7321 taccaacgat tgcccccaat acacaagacg ttgatatcgc agcgataaat ggatatttca
     7381 gaggtaagtt cacaccgaac atggctggtt cagtaacacc taacatacca gaaatacaag
     7441 atgttaatgc caagccttct tctttaacca ttttacgacg tttatagaca aaccatgctc
     7501 caaatgctgc agagccctga caaatattgg aaatcgcaac aattggccat aaatacgtac
     7561 cgcctaagct gctacccatc aattggaaat ctactgctaa aaacatatgg tgtagtcctg
     7621 taattacaag tggtgcatat aacaatccat atattgctcc gccaagccat cctgcatgtt
     7681 ggaatataaa tgtaacacca gatgtaatac ctgtaccaat caataacgca actggtccaa
     7741 tgataataaa tgctaaaaat ccagtaacta aaagcgctac gggtccaacg accaacattt
     7801 ttatcgaatc gtgaacgact ttatttaatc ctttttcaat tttagctaga acgtaagctg
     7861 caattaaaac tggcaacact tgaccttggt aattcaactg cttaatctct aagccaaata
     7921 agttccacgt cggaatattc ccttttgcca aatcatactg agatactaat tgcggatgca
     7981 ttaaaatcaa acctaagact atgcctagaa tcggactacc accaaataca cgcatactac
     8041 tccaaccaat taatgctggt aagaaaataa atgccgtact cgcaatcaca ttaatgatgt
     8101 ttgaaatatc agcaatttgt ggatacatct caataagtgc ttttggacca aataaacctt
     8161 tcattgtaag taaattattg attcccatta acaaaccagc tgtcacaatc gcaggtaata
     8221 ttggtataaa aatatccccc aacaatttga tcaaacgttg tactggattc cctttttgtg
     8281 cagctgcttg tttcgcttca tctttcgaag cttcttgagc acctgtttca tcaataaact
     8341 gcttatacac ttcatcgact gttcctggac caatgacaat ttgatattga tggtctgctt
     8401 taaactgccc cttgaccaac gcgttattac ttaatgcgtc tttatcaact ttactttcat
     8461 ccttcagcac taaacgtaat cgtgttacac aatgcgttgc agcttcaaga ttttcttttc
     8521 ccccaatagc ggttacgatg gcttttacat cttctctttt tacagccata tatttcactc
     8581 ccttaatgtc ttaaaacgag tataacttgt ctagacaagt tatgtaaagg ctttcaacaa
     8641 aatattttta ttttgtcaca attatctggg gtagccattt cagaaaatca atgagccata
     8701 acgacatatg tagcaataac tcctcgctac aattgcgtta ttaacaatac ctcaccacgc
     8761 tctcaccatt ataaagtgta gatgcatggc caactcctaa attgaaaaat gcatatctat
     8821 aaatagcaaa cttgtccaca caagccatcc atcatatttc acaaaacaaa aaagacattt
     8881 agctatgtat attgcacaag ccaaatgccc ttccaatctc aatgccttat catttctatt
     8941 atcaaaaatt acgagccatt caaagacaaa taaaaaaggt aaatactcaa aatttgagca
     9001 cttaccttca ttatatatag aatttaatat aaatattgat ggcggaggaa gagggattcg
     9061 aacccccgcg agccgttaag cccctgtcgg ttttcaagac cgatcccttc agccggactt
     9121 gggtattcct cctaaacaca atatttatca tatatgaacg ttctgtaaaa gtcaatattg
     9181 tttttaagca ttacatgatt accacaaatc attgtcttcc actacagttg acctataaac
     9241 gaatgtaaaa aatcactact tataattaat ctatttgttg tcttacaaac ctttgttttt
     9301 aggttacaaa gatggcatgt gacgttgata gatactagat tttttcaaac tcgattcagc
     9361 atgtcacaac gtcaacgtaa ccaccatttc catacaaaga ttatacacaa actttactag
     9421 ctaaatactg atctactgct ttcgctacgc ctctaccttc tttaattgcc catacaacta
     9481 aactttgacc acgtctagca tctccagcag caaatacctt ttcattatta gtttgatagt
     9541 ttgtatcatc cgccacgatt cgatttctat ccgttttaat gttaaaagca ttcggtactg
     9601 ttggttctgt accttcgaag ccgattgata ataatacaag gtcagcaggc caaaatcttt
     9661 caggtccttc tttcatgacc ataccatttt cgccttgctc taaaatttga gtatacaaac
     9721 cacgtatgtg tcctttatcg tcaacatcgt aacgcattgt ttgaacacca tatgcacgtg
     9781 gttccttacc aaacttagct tcgtactctt ggtgcgcata gtccatttta aacaccggca
     9841 ttgctaaagg ccatgatgca ttttctgtaa atgtaattgc ttctggcaat ttcgtatatt
     9901 tattaaattg aacaatcgat ttacaatttt ctcttaatgc tgtcgctaca cagtctgccc
     9961 ctgtatcacc agcaccaatg ataatgacat tcttatcttt tgcagttatt gttatatcat
    10021 caatttctcc atttaacaac tgcgtttgtt cagttaaata atccatagcg aaatgtatac
    10081 catcacccat gcgtccttct aaaggtaaat ctctaccttt ttgtgcacca gtacataata
    10141 taatggcatc atactcagat tctaacgttg ctttatcaat atcaacgccg acttcaacac
    10201 cattaatgaa agtaatgccc gcttcttcca ttaacttaat acgacgtcga accacatctt
    10261 tatcaagttt catattcgga ataccacaca ttaataaacc gcctgattct ctagcacgtt
    10321 cataaatagt tacttgatat cctagtagat taagttcttc agcagcagct aatcctgctg
    10381 gaccgcttcc aacgattgcc actttttcat ctctacggcg actcggaact ttcggcgcta
    10441 cccaaccatt ttcaaaagct tcatcaataa ttgtgcgttc aataccttta atcgcaatcg
    10501 attctctatt aatcttcatc acacaagcac tttcgcatgg tgcaggacat acacgccctg
    10561 taaagtcagg aaagttattt gtttcgctta agcgttcata agcagtttta aaatcttgat
    10621 gatacactaa gtcgttccat tcaggaatgt agtttccaat tggacaacct attgtttccc
    10681 taccatactg ttgtccggtt tgacaaaacg gcgttccaca atccatacat cgtgcacctt
    10741 ggatagaggc atcttcttta gtaaatcgtt gttgatatgc tttatgatgc ttcaaacggt
    10801 ctaccagtga taattcacct aagtactgtt tgtcatactt cataaatcct ttaaattcac
    10861 ccatcgtatc tccccctttc cttaatacac aacggctggt ttatgtttag catcgattgt
    10921 tttactgtca tcgtaaaatg cagctaacat cgcttcatct tcattgtcat gtaatgattt
    10981 gtgcaaatga attttttgca tcattaattg ataatcttta ggaataactt taacgacgac
    11041 atcttcaatg cgatcaaaat gttttaacac atgaatcgct ctcgtactat tcgtgtgtga
    11101 cacatgttct tccagcattt gcttaatgaa tgctttttct tcttggtgtt taatctttgt
    11161 aaacgaaagc gtatctagtt gattattttc aacaaaagct tctacatcag acgggataac
    11221 gtaagcaata ccaccactca taccttgacc gaagttctta cctacatcac ctaaattaat
    11281 gacatgtcca ccagtcatat actctaatcc atggtcgccg ataccttcaa cgacaacatc
    11341 tacaccacta tttctaatac agaatctttc tcctgcacta ccgttaataa atgccttacc
    11401 acttgtcgca ccatagaatg agacgttacc agcaataatt tcattttgtc gttcttcaaa
    11461 aggtgctttg acaatgaccg taccaccaga taatccttta ccaacatagt cattcgcatc
    11521 tccagtatga tgaatcatta agcctttcgg tgcatatgct gcaagacttt gaccagcatg
    11581 accattcgta taaacattaa ttgtattttc aggaagtcct gcttctccat attgtttcga
    11641 aatctcacta cctgtaataa cccctacatc acgttgttca ttatttactg taaagctacc
    11701 tgtatagcga cgcccttcag caatatatgg cttcgttact tcatataaat ttgttaaatc
    11761 aaatccatgc tcaagattat gattttgttg aatttctttt gtgtttggcc catcgaaagg
    11821 acataacagt ttttcaacat caatactagc cgctttgcta ttcgctttta atgttgatga
    11881 tcgttgtaat aaatcagttc ttccaactaa gtcttctaca cgtttcaaac ctaaagatgc
    11941 taaaatttct cttaattctt gtgcaataaa atgcataaaa ttaacaacat gatgtgcttt
    12001 acctctatat aaagcacgta aatctttgtt ttgagttgca actcctactg gacatgtatc
    12061 tttatggcat acacgcatca taatacagcc caacaccact aatggtgcag ttgcaaatcc
    12121 aaattcttcc gctccaagcg cacatgcgta cgctacatct ttaccagtta ataacttacc
    12181 gtctgtttct aacttaacac gacttcttaa gtcatttagt tttaatgttt gatgtgtttc
    12241 tgctaaacca atctcccaag gaacaccggc atgctgaata ctcgttttgg gtgaagcccc
    12301 tgtaccacca tcgtaaccac tgatgacaat tttatctgca aatgcttttg ccaccccaga
    12361 tgcaatggta ccaacacctg ttttcgaaac taattttacc gcgatatctg catctttatt
    12421 cgcatttttc aaatcatgta tcagttgcgc taaatcttct attgaataaa tatcatgatg
    12481 tggcggtggt gaaatcagac cgatacctgg cgttgaccct cttgtcttcg caatccacgg
    12541 atatacctta gtaccaggta attgaccacc ttcaccaggc tttgcacctt gcgcaacttt
    12601 aatttgaatt tctttggcat gttgtaaata atcactagtt acaccaaaac gcccagaagc
    12661 aacttgttta atcgcactta ctttgttgct tccatcaact tgtacttcat aacgttttgc
    12721 atcttcgcca ccttcaccac tattactctt tccacctaat tggttcatgg cttgtgctaa
    12781 cgtttcatgt gcttccgctg aaatcgatcc ataactcatc gcccctgtat taaagcgttt
    12841 gacaatgtca cttaccggtt caacttggtc gatgtcaatc ggtgtacatg ctttaaattc
    12901 aagtaaatgt ctaatgtgat ctgttctatt tttgttcacc gcttcagagt atgctttaaa
    12961 ttgcgcatag tcattttctt tacatgcgtg ctgcaataag aaaatagatt ccggattaaa
    13021 agcatgatgt tgaccttgtt gtctccattg gaatgtacta cctgatgcaa gataattatc
    13081 atcactttgt tgacgtgctt tattttcagc atcaatttga tcaatcgaaa taccagataa
    13141 cttagactgt gtcccagtaa aataacgatc aatcacatca tgagacaagc caatcgcttc
    13201 aaatatttgt gccccttgat aactttgcac tgtcgaaatt cccatcttag ccattacttt
    13261 aatgacacct tctgacaata catccgtata tgtcttaaca ttatcgacaa cggtgccttg
    13321 taacccttct gtcaatgtca gttgttcaac tgtacgttgc gctaggtatg gcacaattgc
    13381 attcgcgcca tatgcgagta aacaagcaac atgatgcact tctcgtgtct caccagattt
    13441 agcgactaaa cttgtagaca tacgtaaatc tgctttaata agtaattgat gcacatgact
    13501 tattgcgagt aacatcggca ttgcaaagcc attgctatca actaatccac tatcatctaa
    13561 cactagaatt tgagcgcctt gctttacagc attcactgct tctcggccta atgcttctaa
    13621 cgcatcttcc aaatcccctt catataccgt tgataaataa gttaatttaa aatgttcctg
    13681 atcaatcgct gctaagtgtg attcattcaa taccggcctt ttcaattgaa tacgatctaa
    13741 aaccgtttcg tcaggtgcta gtaagttacc ttcgccacct aaataagaaa gttcactcgt
    13801 tacgattttt tcacgatacg catcaattgg tggattcgta acttgtgcaa acagctgttt
    13861 aaagtaatta aatagtgatt ctggtcgctc gttcaacact gcaattggcg catcatatcc
    13921 cattgcaccg ataggatcct tcttaccttc tacaagttcc tgaatatact tatgaatctc
    13981 ttctttcgtg tatgcaaact gacgttgtaa tttaaataac gtctcatctt tccattgcga
    14041 atcttgatat tgtatatttt caaaatcaaa gtcaacttta tggttatcaa tccacgcttt
    14101 atatggtaat tctccagcaa tcgcaccttt taaatcatta ttttcaatga ctttattctg
    14161 tttaaaatca acaagcaata actttccagg attcaattga cctttaaaag caacattact
    14221 ttcaggtacg tccacaacac ccacttcaga tgaaaagaca ataaagttat ctttagtaat
    14281 cgtataacga cctggacgta atccatttct atctgtaagc gcgccaagtt tgtcaccgtt
    14341 acagaacgaa atcattgtag gaccatccca cggttccatt aaataactat aaaattcata
    14401 aaacgcacgt acatttgcat cattcgcttc attatataac caaggttcag gtatgagtaa
    14461 catcgctgcc ttttctggct ccatggctaa cgataagaac tctagcgcat tatctacaat
    14521 ggcagagtca ctaccatcct catcgacaat ttgaaacact ttatgttgat cctcgccaaa
    14581 taatgtttcg attaatttat gttggcgtgc tcgcatccag tttacattac ctttaatcgt
    14641 gttaatctca ccattatgca ttaacatacg gttaggatgt gcccttttcc aactcgggaa
    14701 tgtattcgta ctaaatctcg aatgcactaa ccctagcttt gattgatata aatcatccga
    14761 taaatctgta tatagttttt taatttggtc tgatcgtaac caacctttat atacaattgt
    14821 tttgcgtgat aagctcgtaa aatacaattc taaatcgcac tgagtcgaat agaactctaa
    14881 ttgttttctc gctaaaaaca aacgcttttc aacatcttca atgtccctaa tatcaataaa
    14941 cacttgttga atgactggca tcgtatctgc tacatgttta gcaatggcat ctttattaac
    15001 tggtacatta cgataaccaa gaattgataa cccttcgcct tcaaaatatt ttttaaaaac
    15061 tacttcatgt tcagaaccta aaatgcgttc tttggaaaaa aataacccca cggcatattc
    15121 accttcacct gggatatcaa agtccgttac atgttgtttg aaaaatgcaa aaggtatttc
    15181 agtcataata cctgcgccat caccagtgat gccatctgcg ccgaccccgc ccctgtgatc
    15241 taagcgtcgc aacatttcaa gcgatttatc aatgatgtcg tgagaccttt tattatccat
    15301 attcgcataa aaaccaatac cacacgcatc atgttcctca cgatagtcat ataagccttt
    15361 aattaatttc tcattgtgca tgatgcacac ccctttttta gaatattctg atattagtta
    15421 ttaatgataa ttactataat ctaaaaaatt tatcttttca atatataaaa tagaagatat
    15481 tcatctaaaa aatagaacat ttatcaataa tattgctata atagttacaa aacactcatt
    15541 tttggaggtg cctatatcgc tatggaaatt aaacaattaa gatattttat cgaggtcgct
    15601 aaaagagaac atatctccga aacagcactt gaactaaaca ttgctcaatc agcgataagc
    15661 cgtcaaatta cattattaga acaagaactt aatgtgtctt tatttaaaaa gcaaggacgc
    15721 aatattacgc tcacatctga agggaaatta ttatttaatg aagctctgcg tattattgaa
    15781 catttagata gcacgattga acaatttcaa agccatggat taactaaaaa taaaagtatc
    15841 tacattggct atgatgagag tgacgtatcc cacatgttac taccacttat ccaaacgttt
    15901 catttacaaa atgatacaca tgtcataccg agcttattag aacatgacac aattataaat
    15961 agcgtattaa atgggaatat agacattggt ttcacggaac ttacaccaga aatacgtaaa
    16021 cacaaacaat tacatatgct acctttattc gaagagcact atcatttata tgcgccttca
    16081 gacgatccaa ttacgatggc gacacaccca ccattaattc agtttgagca tagtcatatt
    16141 tattgcttgg caccatttgc cgaaactgta aaaaaacaat tgcgcaagat tactaaatca
    16201 gatgtataca caatcagtag ccaaccactt gcgcagtatc tccttcgtca aaaagaaggt
    16261 tacatcattt catcacaaaa tatacattta ccagaatcta aatcatggat agatataaaa
    16321 ttagatcata ctgaattaaa aagaacgata tgtgctatta caaaggagcc ttatacaaag
    16381 agtgatattg gcatattatt aacacttatt cagcaattaa tgactaaaac gtctacattc
    16441 cattaagtaa ttcgatgcta tcaatgatga ttttcgtttt acacttagca gtaatatctt
    16501 tgaaaataaa aaattaaggg cgtataatca ccatattaat tcattcatag aattggggtt
    16561 ttgtttttga aatatagaaa tttatttaca aagccattta atataatttt aggtttgttc
    16621 tgcatcgcat tcattggtgc tattttattt acattcaata atgagaactt ttacaacaaa
    16681 ccaattggtc aaattataga tgtcaaacat gtttcttcga cacctacaaa agatgcacaa
    16741 aataatcgtg atattaaata taaaaatcaa ttaaaagtca aaatattaaa tggtcaattt
    16801 gctggagaaa cgacgacgat taatcatcag tacgtcaaat cgcaagcaga ttctgaggca
    16861 tttcgcacac acgaaaaagt attattacat atttctaaca agccgagtga tgcttatatt
    16921 atagaaaaga agcgtgatac cttaacggtt atcatcactg gtttgttctt attaactgta
    16981 ttattggttg gtcgcaaagt cggactgcaa tcgattttat cgttaatctt gaattctatt
    17041 gccatattaa tcgcaattta cattcatatt caacatagca atattaattt attcttactc
    17101 atgaccattg ccatgatctg ttcaacaata ctcactttat tgctcgttac aggctggcat
    17161 atgcgtacac tcattaccat agccagtaca attatcggaa cttttttgag tataggttta
    17221 acagaattaa tcatatatat ggcagatggt aaaggcatta aatatgaaac gatgaacttt
    17281 ttatcattac caccgaaaga tatcttccta gcctcagtgt taataggttc acttggcgct
    17341 attatggatg ttgcaattac aattgctagt ggtatgcatg aaattttaca gcgtacgcca
    17401 cacatttcaa tgaggagatg ggcgcttgct gggcgaaaca tcggccaaga tattatggga
    17461 acaatgacta acatcttatt attctcatat ttatccggtg cactacctat gtttttaatc
    17521 tttttaaaaa atgccaatac agtgacttat actatttcga tgaattggtc gttagaaatt
    17581 gcgcgcgcac taacaggtgg tataggtatc gtacttacaa ttcctatcac aatattgttt
    17641 atggaaatat ttgaaacatt aaggagggcg aaacaatgaa tgctgtattt atattggcgt
    17701 tattgctcct ctttctgatg ataattttcg gaggtaaaaa gggattcatc tcttatttaa
    17761 ccctattttt aaatttcgct attttaatta ttagtattgt attgattatt tttggcgtac
    17821 cgatttatgt cgtcacattc tttttctgca ttattattgg cgcttgtaat ttattcgtac
    17881 tcaatagtta caatgttaaa acacaagctg catttatcag cactatcgtt acaacaatat
    17941 tgttgattac actcatttat ttatcggtct acttcggtca tctacaaggc ttttcaaccg
    18001 aacaacaaga tgaaacatat gtgtattcaa tgaatattgg cattgatatg gttcaattta
    18061 tggtgttcac tattattctt gctgttattg cagctgtgat agatttaaca attaccatta
    18121 gttcacccat ttatgaattg catcaagtga atccaacgct tacgcaatac gaattattcc
    18181 aatctggcat gcgtgttggt agagaaattt tagcgacatc tgcgaacacg atttaccttg
    18241 ccttttttgg tggccaactt gctttattta tttggttttt caaacttaaa tattcatttg
    18301 gtcatattat aaactcgaaa atatttgccc aagaatttat ttctattatt ttaggcggta
    18361 ttgccgttgc aatcagtatt ccgattacgg cttggattac ggcattttta ataaaaagac
    18421 catctcgaca gcgcaaaaat gatgagtcta tcaattaatc gcaactagca atataactta
    18481 agttaagcga cctgtggata tatgtcaatt gttcactagg tcgcttttta ctatagtaaa
    18541 ttgtccgatg atctataaca aaaagcacct ttattcatca ttaattgttg tggtcgacga
    18601 tgagtaaaga tgcttttaaa tttaatttat tttatagtta acgccataat atgaacaggt
    18661 cttccgacga acatactatc tcccatatat tgatatcctt gttggcgata aagtgccatg
    18721 gcatgtggat tgtcagtatt aaccgttaac acaatctcat taatatcctg aaatgttgaa
    18781 gtgataaatg acgccaattt ttccattacc actttaccta ttcctctatt acgataacgt
    18841 tgatcaacac taaatgacct gaaaaatact gcatcttggt tatcgctaaa tggtgcgacc
    18901 ccttttccac gatgtaatgt aaaaaaggcc acacattgcc tgccatccat tacaacgatg
    18961 ggataccttt cattatcaga cattgcagct gtaatatttt cctctggcgt tttaacaaac
    19021 ttcaaatcac tttcacttaa ttgaaaagct tctaacatcg aataatcttg tgggacataa
    19081 tgtcgtaaca tgatggtgtc attatttgtc atgtttacca ccataagttt caatccaaac
    19141 tttgacagca ggcgcaatca acgcatcatt atctttatca atccagcgta tatccgtaat
    19201 ttcattattg atagttacgt tttcccaatc gattttggtt aatgcgcgaa atccatttaa
    19261 ctcagttaac atatcctgtt gtggatatgc aggacctaca attgtcccga catattcaat
    19321 ttcatccatt gttaatgtta aatttaattc ttcttttact tctcttaaca gcgcgtgtac
    19381 ttgtgattcc ccttcttcta ttttaccacc cgggaaataa tacttttcgc gattccttac
    19441 ttgaacaagc aatattttat cagctgtttc ttcaactaaa cagacacatt taatcatctt
    19501 tgacacccca actttgtgaa atcaattttt caaattatac tgtacaatta tgttatcata
    19561 tatgagtagt tatagcgcaa aacgttagca attcagcgca cccaactttt catataaaca
    19621 gaagatacta gggggaatta ttattatggc aaaacgttcc aaatcacaac gtttatcaag
    19681 tttactaaat gtcgcaggtt tcatagtcga cggctacaat ggctataaat atcatgctaa
    19741 aaataaaaaa ttagtatatc tttcattagg tttaagcact gtaggaaccg tgttagactt
    19801 ttacatttca attaagtcac cacgtaagtt caaaaaagca gtggcagttg ttactttaat
    19861 aacaaacggt gctagattat ttacaagcat tcgcaaagta aaacatgaat actaattcag
    19921 aaaaggattg gtcgaacata gaacatgaag ttcattcgac caatcctttt tcatatacaa
    19981 aaattcctat tacaacaatc acgactttga tagccccgcc ataaaattta ggattcaatc
    20041 caacttttca gcttgtgaaa tgtaatagga attcattata tatttatata cgtaagactt
    20101 tagtgaatat atctataatt atttacttgg taagctggta ccgttctgta agttaaaata
    20161 cctggtgcag ctgaatagtt catttctgaa actaaaatac taccatcgtt atttacacgt
    20221 tctacaaaca taacatgacc atagtaacct acatctgttt gagcgataga acctacagta
    20281 ggtctattgt cgatagtgta accatctgct gccgctgcgt tatcccagtt attagcattc
    20341 caccaataag tactaatacc tttaccaatt tcagcacgac gattaaatac atgatatgta
    20401 cattgacccc atgtatataa gttttggtga ctgaatactg gtgtattgta acctctattt
    20461 gtcgttgttg cagatcctga gttcgtagat gcattaccag ttactttcaa tttttgaccc
    20521 ggatatataa agaaattatt taagccattt aagctcataa ttttttgata agttgtacca
    20581 tattttgatg caattaatga caatgagtca cctgcttgta ctgtatagta tgatccgcca
    20641 cctgagttcg ttgatggacg gctactattg ctcgcagcgt tacttgagct agcagtacct
    20701 gatactttta atttttgacc tggataaata aagaaattat ttaaaccatt aagtcgcata
    20761 atgttttggt aagttgtacc atattttgat gcgattaatg ataatgagtc gcctgcttgt
    20821 actgtgtagt atgatccgcc acctgagttc gttgatggac ggctactatt actcgtagaa
    20881 ttacttgagc cagatacttt tagtacttgg tttgggaaaa ttagattaga tgttaaattg
    20941 tttaatgact ttaatttagc aatcgaaatc ccatacttat ttgaaattgc ccacactgat
    21001 tcacccggtt ttactgtgtg agttgtagcc gcatttgctt gagttgccgc aacagcgcta
    21061 atcgcgcttg tcccaataat agctgcaatt actttttttt gcactttaaa atcctcctct
    21121 tgcttaactt tcctaacatt cttttatccg aatttatgaa tactacatca ttatacgatt
    21181 ttattatgta taataggttg atgtttgatg acattatgat tacaaaaaaa tcatatactg
    21241 tatcatcaaa ttttataatt accccttaaa attattacaa cttattagat tttacaatat
    21301 ctaaattatt acaatttcat aatatttcac tataaaatga ttacaatccc tttctctatt
    21361 ggaaataatt ttattctcca acaataacgc cctacaaaca taagcatgaa ctttgcttgt
    21421 aggacgcaat ataatttatt ttgctaatga cgtttctatt gccttaatct catctttaga
    21481 taaattaaca ggtttctctc catctttgac atcttcctgc aacgcttttt gagcttcttt
    21541 tgaatgatac aattctacga ttttagcata tgttttgtta tccaagtctt tgtcattaac
    21601 tgcaacaata ttaatatatg gctttactgc atctgaattt gatttttcta aaaatatcgg
    21661 atcattttta ggatctttac ccgctttagt tgctacaccg ttattaataa ctgcaatatc
    21721 gacatcagat aaagcacgtg cagtttgttg tgcatctact gcagtaattt ttaaatgttt
    21781 tggatttgac gttatatctt tcaccgtgcc tgctaatccg aaatcttttt tcagttttat
    21841 taaaccagct gcttctaata gtttaagtgc acgtgcttgg tttgacacat catttggaat
    21901 gacaacttta gcaccatctt taaccttttt gacatcttta attttatctg agtaaatgcc
    21961 caacggtgct aaaactgttg tacttaatgc tgaaatcttt gttcctttat gcgccttttt
    22021 atattgatct aaaaatgcaa aatgttggaa tgcattcata tcaatatcac catcatttaa
    22081 tgctttattc ggtaaattgt aatctgagaa gtgcttaatc tccacatcaa tatcatcttt
    22141 tttagctaat tctttaacct tctcccaagc cttagtgtca tttgatgcga caccaattgt
    22201 tactttttta tcattgttac caccacacgc tgctaataat acaagtgcta cgataactaa
    22261 cccaatcaat cttttcattc tatcaattcc tttcaaaatc ttcactatat atcattaatg
    22321 tctacgtatg aatctagcta gaacattccc tagcgtttga atcacttgga caataatgac
    22381 taatacaata acggtaataa taatgaccgt cgtatcaaat ctttgataac catacactaa
    22441 agctaagtct cctataccac caccgccaac agctcctgcc atcgccgtac ttccaataag
    22501 tccaataatc gcagtggtaa ttgctaatac taacgaacct aaagcttcag gaattaaaaa
    22561 atatctaatg atttgtagtg gtgaagcgcc catcgctttc gccgcttcaa taatcccctc
    22621 gtctacttcc aataatgagt tttcaacaag tcttgcaatg taaggtgcca catatactgt
    22681 taaaggcacg atggcagcag tcgtaccaat tgaagtacct actactaatt ttgtgaatgg
    22741 cacaatcgca attaacaaaa taataaatgg tagtgacctt aaaatattga ttaaaggatt
    22801 taaaacttga tgtatcacta tattgggcca tatgccttgt tttcgagtaa ttaccaataa
    22861 gacacctaat ggaataccaa tcactgctcc taaaaataaa gcaatagata ccatatatag
    22921 cgtttcgtac aatgcttgta ataactgtgc actgtctaaa tcagaaccaa acatatgtta
    22981 atgcacctcc tcaaattgaa tatttttctc tttgaaatat tgatttattg ccgtgtcttc
    23041 aaattgttga tccatattaa atcgaagcca catataacat acggtgttac cttgtatttc
    23101 tgacatagat gaaaataaaa ttttaacctc tctgccacaa atttgaatca agtcatttat
    23161 aatcggttgt gtcacctgag tttcctcgac gaagatttta taatctttaa aatcgccaac
    23221 ttgttcgtca ttcaatcgac gaatcaatga tgtacttggc tcagtctgta taactgtaga
    23281 cacaaaattt tgagcaatcg tcgttttagg atgactaaac acctctttaa cagttcctgt
    23341 ttcaaccact ttcccctttt ccattacagc aacacgatta caaatgtctt taataacgcg
    23401 catttcatgt gtaatcatca taattgtaat gccaaaggtt tgattgacat tctttaataa
    23461 cgtcaatatc gaagcagtcg ttgctggatc caatgcgctt gttgcttcat cgcataggag
    23521 tattttcgga ttagtaacaa gcgctcttgc aatagccacc ctttgcttct gcccaccaga
    23581 taattcatca ggaaattggt cttttttatc actcaatcct acaaattcaa gcatttccgt
    23641 tactcgttgc ttaatttctg ttttgctttt cttacttaaa atgagtggca ttgctacatt
    23701 tttaaatacg gtagctgaat ttaataaatt gaaatgctga aatatcatac cgatatcttt
    23761 cttaatatcc ctcatcattt tatcgctata attcgtaata tcatgtccat ctacaatcac
    23821 ttgtccattc gaggcagctt caagatgatt cacgagtctt accaacgtac tttttcctgc
    23881 accactatat ccaatcacac caaaaatatc attgcgattg accgtaaatg atacgtcctt
    23941 caaagcatct attttttgct tctttttatt aaaggtctta cttacctgtc gaaactcaat
    24001 catcatcgcc cctcctacaa attcttatta atccactagg aataagagca ttatatgtaa
    24061 aattgcatat atcgtcaata caatttgccg aattttctaa aaaattaaaa aataagtaat
    24121 tcatgtgaca atgacgaatt gtgagactac tatgacattt atcaaattaa atccataaaa
    24181 atgtccacca atcctccaca acgcaattac taaatattaa catcgcacaa aaaagcacta
    24241 gcatattcaa gaacaacaaa cgttgaactc aaaatatatg ccagtgctgc tattatttat
    24301 aaagtatcta gtgcttgttt taaatcatcg actaaatctt cagtatcttc aatacctaca
    24361 gaaattctta caagtccgtc tgtaatacct tctttagctc gaatatctgc tggaatggat
    24421 gcatgtgtca tcaatgcagg tactgaaatt aaactttcca ctgcacctaa actttcagct
    24481 aatgtgtaat acgatgttgc tttaatcaat tgtttggcac tttctgtatt tttcacttca
    24541 aatgcaatca cacctgtatg gccatccgct tgagccatat ggacatcatg atttaaatga
    24601 ctttcaatac ttggatggaa cacttgttgc acagctggat gtgcttgtaa cattttaata
    24661 atttcaataa cgctgcgatt aatttgttcc atacgtaaac ctaatgtttt aatacccctc
    24721 acaagtaaat agctatcttg aggtcctaaa atgccacctg ttgaatttga aataaatgct
    24781 aaacgttctg caagcttgtc atccgatgtt gcaactaaac cagcaacgac atcactatgt
    24841 ccacctaaat atttcgttgc agaatgtaag acaatatcga tacctaaatc taatggattc
    24901 tgataataag gtgtcataaa tgtgttatca acaactgaaa tcaaaccgtg ttctttcgca
    24961 atttcagcag actttttaat gtcagtaaca cgtaataatg gattagaagg tgtttcaata
    25021 aacaacatct ttgttgttgg gcgtatcgct tgtacaattg aatctgtatg cgttgtatct
    25081 acaaaatcca cttcaatgcc aaatcgtgta aatacttttg tcaatgcgcg ataagtaccg
    25141 ccgtatacat ctgaatttaa aataatatga tctcctttgt ccaacagcat aacaactgca
    25201 ctgattgctg caacacctga actaaatgca aggccatgtt tgccattttc taatgtcgca
    25261 ataacgcttt ctacagaact tcttgttgga ttcgcagtac gagaatattc atatccttga
    25321 cgtaaatcac caatatcatc ttgtaaatat gtacttgttt gataaattgg tgttgtaacg
    25381 gcacctgtat aatcgtctgt tgtgtgccca ccatgaatta atttagtttt cttgttcatt
    25441 attattctcc tcataattaa atatttgctt agacatatat cgatcactac catctggaaa
    25501 tacgacaaca atcgtacctt cagataattg cgcttttaaa ttcaatgcac cttgtaatgc
    25561 tgcacctgaa gaactgccta ctaacaaccc ttcatttata gccaaacttt tgacatttcg
    25621 aaaggcatct tgatctttaa tcgtaaatat cccatctaca agacgtctct ctaaaaatat
    25681 cggccatttc tcagaaccga taccttcagt gtcatgtgca tgagctggcc ctccatttaa
    25741 cacggaccct tctggctcaa cggcataaca ttgcacgtga tgttgcttta aataacgtgc
    25801 ggtacctgta aatgtaccgc cagagccaat accagccaca aaataatcaa tttgctgtaa
    25861 tgctgaagtc aattcgggtc ccaatgtatg aaaatatgta tccggattat gttcggattc
    25921 aaattgattc atataaacgg caccatattt ttcagcatag gaacgtgcag ctaattgtgc
    25981 cccatgcata ccttcagact gactcgtcct tgaaacttct gcaccaagcg ctatcataat
    26041 attaatcttt tcttctgaaa aaccatacgg cgcaaagatc ttacatttca aatgatgtct
    26101 attcgctgca atagctaacc ctatgcctgt attaccagca gtcgcttcaa caatagtttg
    26161 acctgcacgc acacgccctt cttgaattgc cttctctact aaatatttcc cgagtctgtc
    26221 tttaacactg cctccaggat tccattgttc aagcttggca taaattttaa ctttatcatc
    26281 actataatgt tctaacagta ctaatggtgt attgccaatt aaatcataag taatcataga
    26341 tgcaccctca tctgacatgc cgatcaaatg aatgaaacct ttcttcatgt ctcaatttta
    26401 attcttactt ttcagataag aattataaac gacattttgt tattttgcaa ttatctaagt
    26461 ttcgattaat tcagaaccag tactaaattt tcaattccaa acaaaaaaac acctgagcaa
    26521 cacaaatact tgtgtgtcag atgcttctat atattaacta aataattgca cgataaagac
    26581 taaaataata acgacaggca tcgcatactt aattaagtaa taccaaccac tgaataatct
    26641 aaatcgatct ttaccaaaat attgttgtaa taatttttta tctaataatt gtcctacgac
    26701 aagcgtagta cctaatgcgc ctaatggcat caatacattc gaaacgatga aatccatatt
    26761 atcaaaaatc gttcccgcac cgaatcttac atcttttaag ataccaaaag ataaggttgc
    26821 tggaatacta atgataaata ctaaaatact accgatcact gcgacttttt tacgttttgt
    26881 attgtcattc ttcgtgaagt tagaaacatt taattctaat aaagaaatag atgacgttaa
    26941 agccgcaaat aagaacagca ctaagaatcc caaatagaat aatgtgccta gatgcatttg
    27001 actaaagacc attggcagta ctttaaataa taatccaggc ccttcttgtg gttcatagcc
    27061 aaaactatgt aaagccggaa atatagctag acctgccaat acagatacaa agatattcat
    27121 aacaacgata gaaatagctg atgacttaat cgtcatgtct ttagaggcat aactcgcata
    27181 agtaatcata cctgtagttc ctaatgataa cgtaaagaat gattgaccta gcgcaaacaa
    27241 gatgccatca gcagtaatct ctgatactct tggttgtaaa ataaatttca caccttctaa
    27301 ggcgccatct aatgttaaag acttaatcac aatgacgatt aaaaagacaa acagcaatgg
    27361 catcataact ttcgatgcct tttctaatcc tttttcaaca cctaacatga caataatcat
    27421 cgtagcgaat atgaatatac cttgccctat aacggttaac caaggatttg atattaccgc
    27481 ttcaaaattc atttcttgga gatgattgat gcgttgaaat ataactaatt gccataatac
    27541 ttgtccgatg taaatgacaa tccaaccacc gataacacta tagaagccaa ataaaataaa
    27601 cactgccaaa ttaccgttcc agccaatgat attgagccat tttttaccag ttaatttact
    27661 atatatttgt gttgtatatg tccgtcccat tttcccaaca gtgaattcca taatgagtaa
    27721 tggcaaccca acaaaaatgg tgaatattaa gaacatagct agaaaggcac cgccgccata
    27781 aatccctgcc atatatggga atttccacat ggcaccaaga ccgattgcag aacccgcact
    27841 agctaaaata aatccagttg atgacttcca ttgtgattgt tgtcttttca tcattcacat
    27901 ctcctttaaa gcgttaaagc gtctacgtgt ttaagtaaca ctctaacatg tatgtaaata
    27961 acatgcaata cacctgcatc aaaattgttt cattaatacg gtagcacctg gatttatcat
    28021 aatataatca actcttgtaa tgtgttgtca ttacgctaca ttaaattttc tggttcaacg
    28081 ctgtatacgt gcgccaatat ctcgccacat acaacaccat ctatatcccc aacatctcgc
    28141 cacataacaa cttcaggtgc aatgcccata aaatttccac gacattacaa tacaaatcaa
    28201 cttctggtat aactccataa tatttccacg acatcacact acaaattaac tttagctaag
    28261 ttccctatac tatcacgcca caaaaaacac tcaaatgaca aatataataa catcgtcact
    28321 caagtgttaa tttttaaaat cttcaattat gttaaagcat tgaaaaagcg aataacattt
    28381 tcttctaaaa tatctctgcc ttctccatgc gtcattaaat gatgcgaatc cttcagacca
    28441 tttaattctt tgtctactga tcctaaatgt tcataaataa aatgtgcact tgtttcatag
    28501 gatggcgcat ctttaccacc aaataatata ttagctggca ttttaataac atttaaattt
    28561 gtcatgatat catcaataaa atgttgaaac ttcgtaattt caccttcata atcatcaatt
    28621 gctgctaatt gcgcactcga tgcttgctga tctaaattta aaatattcga catgcgttga
    28681 ctataataaa ctaaatgttc tattaagcca tcgtcactct tttcctttgg tgctgacatg
    28741 acagcgatac gtttcaaagg atagtgttgc gccaatttta atgtcattaa tccacctaaa
    28801 gacacacccg ttgcactgat agattcataa ccttcattga ctaaaaattg gtaagctttc
    28861 tcaacttctt cccaccaatc atctacatta tatgtcatga aatctttcaa caataaacca
    28921 tgacctggat aattcggtgc ataacaacta aatccttggt catttaactc agctgcaaga
    28981 tgcttcacat cccgatttgt acctgtaaat gaatgtaata ataatatcgc atgtccattt
    29041 gtgcctttta aatacgatgg actcggtgtt ttaattctca tttttctata tacctccact
    29101 atgtctaaag atgtttgcta aacgcgttgt cgtcgatgat taataaagta tgggtatacc
    29161 attaagaata acgctaccca aatgattgct agtgacgtgc cgccaatgac atctgaaaag
    29221 taatgtgcat gaaaataaag gcgacaaaat aatatgctaa gccataatat tcccataacc
    29281 aacgcactca acacttttgt tattgtctta gcagcaagtg aaataataat gatcattaag
    29341 gcgaaatata ataatgtgct ggcgttggaa tgtccactcg gaaatgaaaa gcctgtatca
    29401 acggctaaat gattatatgg tcttggacgt aatacagtat ctttaattaa tttgttcatg
    29461 atgacacctg aaaccaaata tgtcacaaac caaaccgcta aatgcctctg tttaataaac
    29521 agtatgattg tgacgataat ggaaatcaac acgacacctt tgacatctcc aatttccgca
    29581 ctaaacgtca tatagtaatt aaacaaattg ttaacatact gacgttgtgg ctcaccgaaa
    29641 taatctgtaa accatgttaa tgatcccata tctatatttt taagccattc ttgatttgtc
    29701 actacactgt aaaacatacc tataaataca atcagcgcga ttaaaaataa aggcactgtc
    29761 attttcggtg atgttaattt tttatctatc atcttacaat ctcctcgtat catcattttc
    29821 attttacaaa tgttatccat aatatcaatg tgccacaaat ttcactttac cgacaatatc
    29881 aaaattataa agttcatatt gttatgtata ttgcaaataa aacattgtat aattgaaata
    29941 acaatatttt gctattttca atttagtacg atttatattt attatacaga gggggtaagg
    30001 cgtatcaata gagttatttt tgtctatata gcgttaatca ttacattagt tagattcttt
    30061 acccctattc atccatcatt ttcaaatttg atttactgga tatttgtatt atattttatt
    30121 cctattatac tatgcgttat cggtttcaag gccgaaaaac ttattgcaac aatggtcatt
    30181 atacctaatt ttttaggaat actttatcga ttatatgcct acgtcacaca tatactcttt
    30241 atgtaaaagg atagtgggca tgtctcgcaa caaatatgct tgcgtcgact tgtcaccatt
    30301 cgcaaatttt atgacatacg cctttcacgg gctatattca gacccacgca ttcatccacg
    30361 taataaacac atcatgtaat agaaaaacag cacacccaaa tatatggcgt tgcgctgttt
    30421 aaccaagcat acttctatag ctttaataag ccagcagaag catacctaac cttcttaaat
    30481 atgcttttcc aaattatcct caagtttgaa tacgataata cgttcacctg taactgtact
    30541 taaatcacta tggaagctca tcactttgat acctgtaatt ttaaaaatga tatcattcaa
    30601 atcttgctca ccggattcaa ctaattcaga acgtgttcgt ttaatattta ataatccttc
    30661 attcgtacta catacacgat attcagctgg cgttaagata ccttgtaaac taataatcac
    30721 catatctctt aaaatgtctg atttaactga caaggaacct ctgccaagga aatctttttc
    30781 ccattgcgta atggctttac tgatttcagc ttcgatttca cctttcgttc ttttcatatc
    30841 actaactcca ataatattaa aattgattac ttcatctttg tatcgttatc acgacatcaa
    30901 ctattacatt aagtttatca tttttagtat attttaagaa gctagaacat tgtagatatg
    30961 atgatatatt agttacttag catcgcaaca tatcatcgtt aaatccaact tttaaaacgc
    31021 ccttcctcat taacgctcat taaacgcagc caatgattag acaccttcct agcgaaatgc
    31081 tcattattcg cgagtagtct tgctacaaca tagtcgggtg cctgaataac gacaagtaaa
    31141 cgaattggcg aatgatacat cgtccgatca gcagccataa cagattgcca tgataagcca
    31201 tacatcagat cactcgcatt accttgcatg acaccaacac ctgacgtgac ggtttgtgtc
    31261 gctttattcc cacttccgta aaaatgcggc gcaactgtcg acgcataata ttgtaaatta
    31321 atccattgtg ccacaagtgc cggaccagaa atgatggtat ttaataatgt gccatcttta
    31381 tctttacgcc aatcataatt gtgtaaaaat gtccgccctt ctaaatcaat gccttttgtt
    31441 aattggcgtc gtccaattat aaatgatgca tttttagcca atccccattc tggacgtacc
    31501 tcactccaat cactcgcaaa ccgctgcgct tcttccactg gatgattcac acgaccaatc
    31561 gttggcagtt tgtccaaacg ttcgcgattc gcgtgttcag aaatcatcgg catcgcgtca
    31621 ttcaatgatt catatgcatc taaagcaata gaagataatg tgtctggcac atatacccat
    31681 gccaacgtat cagtagacgt atgatgttct gctaccgcaa aaacagttgt ctctggaata
    31741 tacacacctg attgttttaa tccttgtctg acatttggac gattacatat catcgctaat
    31801 aacttagcat taaaaccgct tgatgcgcca ccacaagccc cacattcaag tgatgcatga
    31861 tgtggattat tgtgagaatg actagcatga cctgctaaca caacgaacgg cgcaaatgct
    31921 tcggttaaat ccatcaattt caacgcttgt aacgcgaaat caatttgctc ttgctcagta
    31981 aatccaacag gtaagtctga tgttcggtca aactcacgat caatcgtcaa ctttgtttca
    32041 ggctttttca accacttttg ttttattttt tgtaaagacg cgcgactttt tctaggcata
    32101 atcgaattga caatggtact taagcttaaa aatggcccac ttaattcagg caataacaga
    32161 ctaggcatga cattattttt catcaattta aatgtgtaaa acatcgatga cattgtctgt
    32221 tgctgttgtc gataaacatt catatcgtag cggtctgcaa attctttaat gcgatatgcc
    32281 ggcggtacca tgacaggtaa tgaatcatgt ttgaattgtt cgtctacggc atctttttga
    32341 ataggtaatc caaagaagcc tgcaatacca atcgtttcaa agggccctgc tgcttcgata
    32401 tgtctacgaa atggttctga acgaacatct atacaaaatg caatttgcgc tttcgttgat
    32461 gtgcccatct gatttagctc gctattattt tcatcaactg cttgtgtgtc atttaacaat
    32521 actgaatgtg gctgattagc gttatcattt tctgagacat ttacttggtt tacatctaat
    32581 gcgcccgcca cactttcatg acctgcttta attttttgtt ttaactgaga ttcgtatgtc
    32641 atttcccagg caattagcca taaattttta aatacatttt tattcatagt tgctgcaaaa
    32701 tgaataaacg tttgaatttc attgacgtca tgttgtagta atacatcgct aggcatatca
    32761 ctgtagtaac accatgatgc aacagtttgc ttaaaccaat tttccgatct actttcacaa
    32821 tctttagcga ctgacttaaa ctcatcacca actagcaatt gttcgacaac taaccgaatt
    32881 gccaaataat ccgttaacaa atgttgttca aagtaatgct gttgtgaacg gtaatacaac
    32941 atacctgccc aacccggtaa cgccaaaaga tgtccttcaa cataagcttg gtagtcttcc
    33001 tgatctattg aaaaatgagt taatactgac tctatcgtca tttcaggatc attgggtaag
    33061 cctttaatca cttggcgctg tgctttagta aaactatggt catgttgcgc taaatgcaac
    33121 catgcatggt aaaaactttg ctcacgcttc ggcattgtcc aactcgatag aaattgatcg
    33181 atataaagtt tcgtccattt aatcatttga cgattcactt gttcgctaag tggctcacct
    33241 tgttcatcta ttattgcatc actcatcgga cgtacatcat agtgatgata tgattcagcc
    33301 atatcacgtt ttgatttttc taatagtaga tcagcaacaa catcaacatt tgaatgattc
    33361 atatatgatg caggtacgtc ttttaatgtt ttaatgttat caatataaag attgatgtag
    33421 tgttgcggga tattgtagtg atgttcaagt aacatatcag taacaagttg attaaagaca
    33481 ctttcatcta attcaccacg tgccacagcg ctttctatta atgctttatt tgggaaaata
    33541 tccacatctc gaacatcacg taaccatttt gcgacatctt caaacgtatc cgcttctaat
    33601 ccttcccatg gatttcgtgc tgcaaaaatc gaaattggtg ataatggtgt aataacacgt
    33661 ttcgcatttt caatgactga attgatattt aactgtgttg tcataccttt cacctcctat
    33721 aaatacttct tcaaataatt cggatgactt tctatcgctt tcgagcgtgc ttcacctaga
    33781 ttaactaacc acacgtacaa taccgcaaaa gccttagagt atcgatgccg cgccacccaa
    33841 atacttaata aactgccaaa gattaaaata acaacactaa tgatgacact cactgtaggc
    33901 ggcgttgtcg catgtgttgt tatattttgt aatacagcgt aaaaataatt atgtgtgatg
    33961 acgtagataa atgtcacgat tgcaatcaaa atcataccaa caagacgtgc catgcgtcct
    34021 ttactaaagg ctaccatttg attccaagat acaagtaatg accatcctag aatgagtgca
    34081 cttaacactt catatgcact tctgtcacta ctcatccaaa atagaaatgc cacgataata
    34141 gctaatacac gtcccatgac aatccagcca taagcgtctt tagcagatgc ttgttttgga
    34201 atattgaatc gcttcacgat agaacctgat tgtaaaaata atgttgcttt aaaaataccg
    34261 tgcaatatta aatgaataat cgctgctgaa tatacaccca atgcacattg aactaacata
    34321 aagcccattt gactcatcgt agagcccact aattggcgtt tatagtcaac ttgaactaag
    34381 ctaatacccg atcctaacaa tacagaaata ctagaaagga taagtaataa tgataacgca
    34441 aatccattat caaatatcgg cgcaaaacga gttagaataa caccacctgc attcacaatt
    34501 cctgcatgca taattgccga tactggcgtt ggtgccgtta cagattcaat caaccatcga
    34561 tgaaaaggaa attgtgctgc cggtatcatg acagctaata caagtagtac attcgtcaac
    34621 aatgaccatg tcggatgaac tatatgttgt ggtacccgcc actcgccagt cgcaatataa
    34681 atagttacaa ttgctccaac gaatgcaagc caaccacata aaaatgtcat gcttgataat
    34741 ttcgcagact cacgtggcac tttccaaaaa cgattaacgt tcatcagcaa tgttaaacat
    34801 aataatgtaa taccccagca gagtgccatc agtcttaagt cttcagacat ccatgctaaa
    34861 gatgcaaacg acgtaatcgc agtgaacaat ggaaagtaat gtctataatg atgatcacct
    34921 agtaaatatc gcattgaaaa cttttgaata ataaagccaa gcgccattac aaagccagct
    34981 aataaccaag ataaacgatc tattttaaat ggacctaaga catgttgacc atgaataccg
    35041 aaaaagccaa tgactgcaaa taatactggc atgactagta tgtataaatg taatttaata
    35101 tatctcattg gcataactgg tgctaaaaac aacaagccac ttatcaatgc aatgataagc
    35161 gcaataacaa acagtgaaaa tagcaattga aaacttaaca ctgcataacc tccttatttc
    35221 taatctctcg cataattgct tatgtataaa aataaaaacc tacaatagta gattctgtac
    35281 ataatggcag aaaatttact attgcaggtt tcagtttaac tagacactgc atcacggtac
    35341 gttgatatac cttgttgcag tgttctcttt aagcgtgctc ccatgcacat atgtatataa
    35401 aatgttactt ctgtctgttc aattcatctt cataaatatg ctttgcctag acgagaccta
    35461 acgtgttatt cgttttaaac ttataacata aaatataatt aaatttctgc ttcatgtcaa
    35521 attcatgagc ttaacctcta ttaaaccaat gattgtaaag attttgtaaa tgcacctgta
    35581 cagttaggca gtatttcccg tccttttaaa ataaaaaatt cgcagttatg atcataacaa
    35641 ttcaagttag gaaaaaaatc aattacgcac aagataacta tgtacaatga agttaactca
    35701 taagcaaagg aggtaatctt aatgggtatc atcgctggca tcattaaagt tatcaaaagc
    35761 ttaatcgaac aattcactgg taaataagat ttcataacaa acaaaggagg tctttcacat
    35821 gggtatcatt gcaggaatca ttaaattcat taaaggatta attgagaaat tcactggtaa
    35881 gtaagttata aaaatctcat agatatgaac atcttatttg aagggggcca ttcacatgga
    35941 attcgtagca aaattattca aattctttaa agatttactt ggtaaatttt taggtaacaa
    36001 ctaatctcaa acattaacga tcaacaactc atcactatgt taaatcaaca tacaggagga
    36061 caaaacgatg gctattgtag gtactatcat taaaatcatc aaagcaatta tcgacatttt
    36121 cgcaaaataa tttaagcgaa ttgaatactt aaaattctca ggccactata ccaataggga
    36181 tggtggcttt gctttgtgtt gatttattga tgtgaggtga gtcttgttag tttgttgcaa
    36241 ataaatggtc ttggtgtttt ttgtatagga cgttcttagt gggacatacg gaatattcgt
    36301 gatctttgta gtctgacgcg ttatattttt gtggcgtgtt ttatgtttga tactcgagtt
    36361 ctgagacatt catgatttgg catgcgaaat cttaatgatt ttcatgatct agcgcaagat
    36421 atattggcca cgtgcggaat tgcgttgcac gtttagactg aaacactcgt gtgaccgtaa
    36481 gtgttaatag tacattgata gctgcattta cttcactcat ttttatgact gttaaacaat
    36541 gattgtacct tcaattaaca gttggtacga tggttttgcc atttttcatc aacgtaaata
    36601 taaaaaggac taagacacat acatgtccta gccctatgga taaaatgcaa atttctgctt
    36661 tatcaaaact atcacacttt agatagattg aaaacaaaaa gatcctaaga acaccttaac
    36721 tttttattaa ttgtcataaa ttgcaaacaa ttaagccaca attcaaaaat gattatactt
    36781 cattcaactt atcgtgctgg tctaatttgc cattgatatg gatcttcaaa ttgttgccaa
    36841 tctgcatcaa tttcttgcgc attgactaag catgcgtcga gttcttttgt taatttttct
    36901 tcatctaatt ctgtaccaat aatgacaaat tgtgtatgac gatcgccata ttctggatcc
    36961 cattcagctg cgacatcttg acgttctgct aatatttgtg tttgttgcgc ttcagacata
    37021 ctagccaccc aatatgtaac tggatgaata ttgcaagatg accctgcttg agataataaa
    37081 catgctacgt gattgtattg tgctagccat acgatacctt ttgatcgaac gacattattt
    37141 ggcatgcttt ctaaccaatc attgaacctt ttagcatgga aaggtagacg acgtttatat
    37201 acaaacgatg atataccata ttcttctgtt tcaggtgtat gcgatgcatg cccaccagac
    37261 tcaagttctt tgatccatcc tgctgactcg ctcgcttttt caaaatcaaa acgctgcgta
    37321 ttcaagactt cttttaaatc tacttcagaa tttgttgtct taataatttt agcagtcggt
    37381 tgcaatgcgc ttaacatttt ttctaacttc gctagttctt cttcactaat taaatcaatt
    37441 ttattaataa tcaatacatc acaaaattca acttggtcaa ttaataaatc agcaatcgaa
    37501 cgctcatctg tttcgtcaac gctttgatca cgatccatca ataaatcttc tgagttgatg
    37561 tcatgtacga agcggttagc atccacaact gtaaccattg tatctaaacg gcaaatcgct
    37621 gtaagatcaa tgccaagttc atcatcaata tatgagaaag tttgtgcaac aggtactggc
    37681 tctgaaatcc ctgttgactc aataacaatt tgatcgatgc cacctttttt cactaaacgc
    37741 tcaacttctt ttaataaatc gtctctaagt gtacaacaga tacaaccatt agaaagttcg
    37801 actaattttt catctgtacg cgatagtccc ccaccatctg cgacaagatc tttatcgata
    37861 tttacttcac tcatatcatt tacaattacc gcgatacgtc gaccttctcg attttgtaaa
    37921 atatgattta acaacgttgt cttccccgag cctaaataac cacttaatac cgtaactgga
    37981 attttagcca tatatatacc ctcacttcaa tttatttgta aataggaata attccgtttt
    38041 acattatata ggagcgtttc ctctttcgca atcttcgata ataaaaaaat agtatactta
    38101 attaaattat tgagcgcttt actttataat ggagacaaag atatatctca cgaaagagaa
    38161 tcgaggtgta taaacatgtt atttgtcatt ttagttttat atgttactgg tattgcattt
    38221 attctactca gtgtttttgg ttcaaagact gaaggattat ctacgaaaca tactttatat
    38281 accattggca gtgctattat aacgattgct attttcattt caattggcta tgccattcaa
    38341 tacttaactg cagcgcttta tggtttgtaa ggtgaaggtg atgagtaacg ggtagttcgg
    38401 gagaggttaa cttgcgttga ttttgataaa gtgatcatag cttttagtac ttgaggattt
    38461 ttattgttgc tgttacgaat gtggtcatgt ttaatgcggg acagtaattt aagttgtttt
    38521 tttacaattg agagtgtgat atttcgattc ggttcgaatt actttacatg ggaataatat
    38581 aaattaaaaa gaagcggcct agtgtcagtt gtgaatatac tgaacattgg tcgctttatt
    38641 tagtagtatg atatgtagtt tagctattaa tttttttcag gtcatcctta atgctgtcta
    38701 tctcagacat ggcactttta acccaatctc cttgagctgc acctttaaaa ttagctttaa
    38761 aagcttcgca atgttgcgcc atttgttcaa ttaatacttt ttcttcacct tttaatccgt
    38821 tttcaatatc tttgtattta tgcttatgtt caggtgcaat aactgtgcga atattttctt
    38881 tttgcgcttc cattttagat atgagattaa gtgtttctac tgtagtactt atatctggca
    38941 ttcttaaggt catatctggt tctattagag tcatttaatc tcctccaaat tatcagtcac
    39001 ttagcttatc taactgcttt tcataagact tttttaagtc ttctttatat tcttctaatt
    39061 tccattcttg ctttctaatt atctcattga tttcttgctc atacgcttct atatttctcg
    39121 tcattttcct ttgggcatct tcactataat tcatttcgtg cttcaaatat tgaaaaacat
    39181 cataactacg ctcattttct tttcgagttc tatgcctaag ttcatttaaa ttttgcagtt
    39241 cattctctat acgttttttc tcagattcat atgattcttc taattcattt ttattattaa
    39301 tttgattaaa catcatagta atccacctat ttgtgatgca agcacttgat ctttatcaac
    39361 gatttcatta atactgagtc taactctcat aatataattt gatacagaag accctatcgt
    39421 tgaaagttgt ctttcttttg cagatatcat ttgttcaggt tctcttacca tactactttc
    39481 attcacttga ccttcatcta aagcattgag aactccattt acactcaaat cttcacctac
    39541 agcttttcca gctttttgag cgtcttccca attttttcca aatttctttt tcttttcatt
    39601 atacattttt ttgatttgat taatttcatc gtctatcagt tgactgaatg actgcgcaat
    39661 tgttaaagct gttaaacttt ctaaaacttt ttgctgcgaa gaagataatc ctcctccatt
    39721 tgtagtcatc atatttgctc taagtaattc tatactagct aatttagaga tagcattatt
    39781 tgaattcttt acaaatgact tattattttc ttctgcataa ccttgtagtt ttttaagttc
    39841 tttttttata gctttttgtt cttcttcggt cagaaagcct tccatttcat gacctttacc
    39901 atttttaaag actttatcat taccgaaaaa aacataatgc ttatatttct tcagattttc
    39961 tgtcaatgca tcattttctg caacaaacct agtaatatta cctttataat ttttatataa
    40021 ttctaataaa cgttttccat ctggggaatt aggattcaaa ctagttatag aaataggagc
    40081 tggattataa accacaatgt tcggaatacc attactcatt cctagaacta ccgcatctct
    40141 tccacctagt gaatgtccag taataaaatc aatgtcatac ttacttttta ttttatttat
    40201 aaattcttgt gtattcgcat atctatagtt ttgatcagat gcaggagaat ataatatttt
    40261 aagatctgca tatccatctt taactgtttc caaagcattg gtaacatttt gtcttgaaaa
    40321 ttcaccttct tttaactttt taaaggcttc gtcttgtaaa ttagtcccag tcatcccgag
    40381 agttactttg cctgtatctt tatttaaaaa agcagaagtt gttactccag taagagaatc
    40441 agtgtaacta tcaacatatt caagattaga agggaatttt ttcatttttt tcaaatcatt
    40501 aaattgatta atcacgtctc tttttgatag accattatta ttagcattct caacttcgta
    40561 tgacgctttc gaaatagcac ttgtagactc agtattttga ttaattgctt ttaaatctat
    40621 tttatacttc ctactcaaca ttctcacctt cagacggtcc aaaattcaaa taatctctta
    40681 atgttgtctc ttgaccttta tcttttgaaa atttatattc tattttttta tatcctactg
    40741 agcttccttt tatatcacca tctatatata attttagatt tggagagtta ttagttgtaa
    40801 ttggataaat ttctctaagt ttttttacat ttttatcact attgttcatt ttatattgtg
    40861 cttcatagct cggtacgttt tcattagttg ttatatttcc atcttgataa ttttttaaat
    40921 ctttaaaatc agcatactga ctaaaaaatt taaaattttc aattttttgt ttaagttttt
    40981 tgtcttctac attatccaaa agaacaatct tattattttt tagttcaaca cgatattttt
    41041 tctcattatg atttttgcta atatcatcat aaactttatt tacaaaataa taacctgttg
    41101 cctttttggc atttctatta agatataaca ccatgccttc atcatctaat actcctggtt
    41161 tgttactttt tgaaaaacct gtaagtatag tccacgtacc tttatcgcct tttttaaact
    41221 caccatctcg atatccttct ttgtcgtata aatcctcgag attcttaatt ggatacatat
    41281 ctaacgtttt cgcaaaactc tttttgattt gttcttcttt tgaatctgtt ttattcgttt
    41341 gttcctcttt tttctgttca tctttcatat ttccacatcc cccaataaaa atgattaaaa
    41401 gtaatacact catgtacaaa gcaatttttt ttatatactc catcatttca catctctctg
    41461 ttttaatt
//
LOCUS       DAKTLH010000009        38423 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.009, whole genome shotgun sequence.
ACCESSION   DAKTLH010000009 DAKTLH010000000
VERSION     DAKTLH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 38423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38423
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.009"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            119..1456
                     /locus_tag="O0394_000378"
     CDS             119..1456
                     /locus_tag="O0394_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCY0498492.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKFLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            1458..2075
                     /locus_tag="O0394_000379"
     CDS             1458..2075
                     /locus_tag="O0394_000379"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCY0498493.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            2103..2432
                     /locus_tag="O0394_000380"
     CDS             2103..2432
                     /locus_tag="O0394_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCY0498494.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            2646..3572
                     /locus_tag="O0394_000381"
     CDS             2646..3572
                     /locus_tag="O0394_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCY0498495.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQNMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            3573..4376
                     /locus_tag="O0394_000382"
     CDS             3573..4376
                     /locus_tag="O0394_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCY0498496.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIEDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            4393..4740
                     /gene="yabA"
                     /locus_tag="O0394_000383"
     CDS             4393..4740
                     /gene="yabA"
                     /locus_tag="O0394_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCY0498497.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            5014..5739
                     /locus_tag="O0394_000384"
     CDS             5014..5739
                     /locus_tag="O0394_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCY0498498.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRAERLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            5732..5980
                     /locus_tag="O0394_000385"
     CDS             5732..5980
                     /locus_tag="O0394_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCY0498499.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            5982..6821
                     /gene="rsmI"
                     /locus_tag="O0394_000386"
     CDS             5982..6821
                     /gene="rsmI"
                     /locus_tag="O0394_000386"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCY0498500.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            7106..9079
                     /gene="metG"
                     /locus_tag="O0394_000387"
     CDS             7106..9079
                     /gene="metG"
                     /locus_tag="O0394_000387"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCY0498501.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKSGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLNES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATKEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9110..9883
                     /locus_tag="O0394_000388"
     CDS             9110..9883
                     /locus_tag="O0394_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCY0498502.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            10050..10586
                     /gene="rnmV"
                     /locus_tag="O0394_000389"
     CDS             10050..10586
                     /gene="rnmV"
                     /locus_tag="O0394_000389"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCY0498503.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHAELIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            10597..11490
                     /gene="rsmA"
                     /locus_tag="O0394_000390"
     CDS             10597..11490
                     /gene="rsmA"
                     /locus_tag="O0394_000390"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCY0498504.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            11590..11853
                     /locus_tag="O0394_000391"
     CDS             11590..11853
                     /locus_tag="O0394_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCY0498505.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
     gene            12163..13011
                     /locus_tag="O0394_000392"
     CDS             12163..13011
                     /locus_tag="O0394_000392"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCY0498506.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            13025..13849
                     /gene="purR"
                     /locus_tag="O0394_000393"
     CDS             13025..13849
                     /gene="purR"
                     /locus_tag="O0394_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCY0498507.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            13866..14246
                     /locus_tag="O0394_000394"
     CDS             13866..14246
                     /locus_tag="O0394_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCY0498508.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNIDGHIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            14318..14620
                     /gene="spoVG"
                     /locus_tag="O0394_000395"
     CDS             14318..14620
                     /gene="spoVG"
                     /locus_tag="O0394_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HCY0498509.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            14810..14941
                     /locus_tag="O0394_000396"
     CDS             14810..14941
                     /locus_tag="O0394_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498510.1"
                     /translation="MRLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            14964..16316
                     /gene="glmU"
                     /locus_tag="O0394_000397"
     CDS             14964..16316
                     /gene="glmU"
                     /locus_tag="O0394_000397"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCY0498511.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            16463..17428
                     /locus_tag="O0394_000398"
     CDS             16463..17428
                     /locus_tag="O0394_000398"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCY0498512.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            17578..18231
                     /locus_tag="O0394_000399"
     CDS             17578..18231
                     /locus_tag="O0394_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCY0498513.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            18542..19114
                     /locus_tag="O0394_000400"
     CDS             18542..19114
                     /locus_tag="O0394_000400"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCY0498514.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            19114..22620
                     /gene="mfd"
                     /locus_tag="O0394_000401"
     CDS             19114..22620
                     /gene="mfd"
                     /locus_tag="O0394_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCY0498515.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNVEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            22610..24136
                     /locus_tag="O0394_000402"
     CDS             22610..24136
                     /locus_tag="O0394_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCY0498516.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILLFIAIFVFANN
                     IAHMMGDGHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLYAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRITGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            24136..25329
                     /locus_tag="O0394_000403"
     CDS             24136..25329
                     /locus_tag="O0394_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCY0498517.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHNQFEDVYIDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVIFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEK
                     GESNT"
     gene            25326..25589
                     /locus_tag="O0394_000404"
     CDS             25326..25589
                     /locus_tag="O0394_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCY0498518.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            25607..25999
                     /locus_tag="O0394_000405"
     CDS             25607..25999
                     /locus_tag="O0394_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCY0498519.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            26104..26505
                     /locus_tag="O0394_000406"
     CDS             26104..26505
                     /locus_tag="O0394_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCY0498520.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            26685..27980
                     /gene="tilS"
                     /locus_tag="O0394_000407"
     CDS             26685..27980
                     /gene="tilS"
                     /locus_tag="O0394_000407"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCY0498521.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKKWIIISKNGDE"
     gene            27985..28524
                     /gene="hpt"
                     /locus_tag="O0394_000408"
     CDS             27985..28524
                     /gene="hpt"
                     /locus_tag="O0394_000408"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCY0498522.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
     gene            28781..30874
                     /gene="ftsH"
                     /locus_tag="O0394_000409"
     CDS             28781..30874
                     /gene="ftsH"
                     /locus_tag="O0394_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCY0498523.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHQQA
                     PNIEKPYDPNHPDNK"
     gene            31103..31984
                     /gene="hslO"
                     /locus_tag="O0394_000410"
     CDS             31103..31984
                     /gene="hslO"
                     /locus_tag="O0394_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCY0498524.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            32163..33095
                     /gene="cysK"
                     /locus_tag="O0394_000411"
     CDS             32163..33095
                     /gene="cysK"
                     /locus_tag="O0394_000411"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCY0498525.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            33311..34114
                     /gene="folP"
                     /locus_tag="O0394_000412"
     CDS             33311..34114
                     /gene="folP"
                     /locus_tag="O0394_000412"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCY0498526.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAINRVKAMIDEGADII
                     DVGGVSTRPGHEMVSLEEEMNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDMI
                     NDQWAGLYDHRMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
     gene            34092..34457
                     /gene="folB"
                     /locus_tag="O0394_000413"
     CDS             34092..34457
                     /gene="folB"
                     /locus_tag="O0394_000413"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HCY0498527.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            34454..34930
                     /gene="folK"
                     /locus_tag="O0394_000414"
     CDS             34454..34930
                     /gene="folK"
                     /locus_tag="O0394_000414"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCY0498528.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK"
     gene            35324..35416
                     /locus_tag="O0394_000415"
     CDS             35324..35416
                     /locus_tag="O0394_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498529.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            35469..36956
                     /gene="lysS"
                     /locus_tag="O0394_000416"
     CDS             35469..36956
                     /gene="lysS"
                     /locus_tag="O0394_000416"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCY0498530.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            37514..37628
                     /gene="rrf"
                     /locus_tag="O0394_000417"
     rRNA            37514..37628
                     /gene="rrf"
                     /locus_tag="O0394_000417"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            37641..37716
                     /locus_tag="O0394_000418"
     tRNA            37641..37716
                     /locus_tag="O0394_000418"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37674..37676,aa:Val,seq:tac)
     gene            37733..37808
                     /locus_tag="O0394_000419"
     tRNA            37733..37808
                     /locus_tag="O0394_000419"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37766..37768,aa:Thr,seq:tgt)
     gene            37815..37887
                     /locus_tag="O0394_000420"
     tRNA            37815..37887
                     /locus_tag="O0394_000420"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37848..37850,aa:Lys,seq:ttt)
     gene            37921..37995
                     /locus_tag="O0394_000421"
     tRNA            37921..37995
                     /locus_tag="O0394_000421"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37953..37955,aa:Gly,seq:gcc)
     gene            38003..38091
                     /locus_tag="O0394_000422"
     tRNA            38003..38091
                     /locus_tag="O0394_000422"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38037..38039,aa:Leu,seq:taa)
     gene            38097..38173
                     /locus_tag="O0394_000423"
     tRNA            38097..38173
                     /locus_tag="O0394_000423"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38131..38133,aa:Arg,seq:acg)
     gene            38194..38267
                     /locus_tag="O0394_000424"
     tRNA            38194..38267
                     /locus_tag="O0394_000424"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38228..38230,aa:Pro,seq:tgg)
     gene            38291..38366
                     /locus_tag="O0394_000425"
     tRNA            38291..38366
                     /locus_tag="O0394_000425"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38324..38326,aa:Ala,seq:tgc)
BASE COUNT        13771 a         5207 c         7778 g        11667 t
ORIGIN      
        1 acaatagaca agctattgca taagtaacac taacttttgt caaagaagtg ttactatata
       61 attaatactt ttgaaagtaa ctaattccaa aacagtgtaa ataaaggaag cgtatatcat
      121 gaagcaacct attttaaata aattagaaag tttaaatcaa gaagaagcga tttctttgca
      181 tgttccgggt cataaaaata tgactatcgg tcatttatct caattatcaa tgacaatgga
      241 taaaactgaa atacctggat tagatgattt acatcatcct gaagaagtca ttttggaaag
      301 tatgaagcag gtggagaaac attcagatta tgatgcttat ttcttagtga atggcaccac
      361 ttcaggaata ttatctgtca tccagtcttt ttcacagaaa aaaggcgata tcttaatggc
      421 aagaaatgta cataaatctg tgttacatgc gctcgatatt agccaacaag aagggcattt
      481 tattgaaacg catcaaagtc cgttaacgaa tcattataat aaagttaatt taagccgttt
      541 gaataatgac ggtcacaaac ttgctgtgtt gacttatcct aactattacg gtgaaacatt
      601 taatgtagaa gaggttatca aatttttgca ccaattaaat attcctgtac tcattgacga
      661 agcacacggc gcgcactttg gattgcaagg atttccagat tctacattaa attatcaagc
      721 tgactatgtt gttcaatctt ttcataaaac gttaccagct ttaacgatgg gctcggtact
      781 ttatattcat aaaaatgcac cttatagaga aactattata gaatatctaa gctacttcca
      841 aacatctagt ccttcgtatt tgattatggc tagtttagag tcagctgccg agttctataa
      901 aacatatgat agtaccgtgt tttttgataa gagagcgcaa ttaatcgaat gtttggagaa
      961 gaagggtttt gaaatgcttc aagttgatga tccgttgaag ttgctgataa aatatgaagg
     1021 ttttacaggt catgatattc aaaattggtt tatgaatgca catatctatt tagaattagc
     1081 ggacgactat caagcattag cgatattgcc gttatggcat catgatgata cgtatttatt
     1141 tgattcgctt ttacgtaaaa ttgaagatat gattttaccg aaaaaatcag tttctaaagt
     1201 taaacaaaca caacttttaa caactgaagg taactataaa ccaaaacgct ttgaatatgt
     1261 tacttggtgt gatttgaaaa aggcaaaagg taaagttctg gcgcgacata ttgtcccgta
     1321 tccgccaggg attcctatta ttttcaaagg agaaacaata actgaaaata tgatagaatt
     1381 ggtaaatgaa tatctggaaa ctggaatgat agttgaagga attaaaaata ataaaatttt
     1441 agttgaggat gaataaaatg tcagctttta taacttttga gggcccagaa ggctctggaa
     1501 aaacaactgt aattaatgaa gtttaccata gattagtaaa agattatgat gtcattatga
     1561 ctagagaacc aggtggtgtt cctactggtg aagaaatacg taaaattgta ttagaaggca
     1621 atgatatgga cattagaact gaagcaatgt tatttgctgc atctagaaga gaacatcttg
     1681 tattaaaggt cataccagct ttaaaagaag gtaaggttgt gttgtgtgat cgctatatcg
     1741 atagttcatt agcttatcaa ggttatgcta gagggattgg cgttgaagaa gtaagagcat
     1801 taaacgaatt tgcaataaat ggattatatc cagacttgac gatttattta aatgttagtg
     1861 ctgaagtagg tcgcgaacgt attattaaaa attcaagaga tcaaaataga ttagatcaag
     1921 aagatttaaa gtttcacgaa aaagtaattg aaggttacca agaaatcatt cataatgaat
     1981 cacaacggtt caaaagcgtt aatgcagatc aacctcttga aaatgttgtt gaagacacgt
     2041 atcaaactat catcaaatat ttagaaaaga tatgatataa ttgttagaag aggtgttata
     2101 aaatgaaaat gattatagcg atcgtacaag atcaagatag tcaggaactt gcagatcaac
     2161 ttgttaaaaa taactttaga gcaacaaaat tggcaacaac aggtgggttt ttaagagcgg
     2221 gtaatacaac attcttatgt ggtgtcaatg atgaccgtgt agatgaaata ttgtctgtga
     2281 ttaatcaaac gtgtggtaat agagaacagt tggtttcacc tattacacct atgggaggca
     2341 gtgcggattc gtacattcca tatccagttg aagttgaagt tggcggtgct actgtatttg
     2401 ttatgccagt tgatgcattc catcaatttt aattctataa tacaatcatc aattagagat
     2461 acttaaaata gtgtattaat aagttattaa caattttggg ttgcttgact gcgactagtt
     2521 cagatgccaa tagatttgat ttttgtggtt ctaaaaataa tcacaaatca tggtcgctat
     2581 tgttgcagta attagttgct ctttggcaac ctttttatat aaaagcaaaa gggagtttgt
     2641 aatgaatgga tgaacagcaa caattgacga atgcatatca ttcaaataaa ttatcgcatg
     2701 cctatttatt tgaaggtgat gatgcacaaa cgatgaaaca agttgcgatt aattttgcaa
     2761 agcttatttt atgtcaaaca gatagtcaat gtgaaacaaa ggttagtaca tataatcatc
     2821 cagactttat gtatatatca acaactgaga atgcaattaa gaaagaacaa gttgaacaac
     2881 ttgtgcgtca tatgaatcaa cttcctatag aaagcacaaa taaagtgtac atcattgaag
     2941 actttgaaaa gttaactgtt caaggggaaa acagtatctt gaaatttctt gaagaaccac
     3001 cggacaatac gattgctatt ttattgtcta caaaacctga gcaaatttta gacacaatcc
     3061 attcaagatg tcagcatgta tatttcaagc ctattgataa agaaaagttt ataaatagat
     3121 tagttgaaca aaacatgtct aagccagtag ctgaaatgat tagtacttat actacgcaaa
     3181 tagataatgc aatggcttta aatgaagaat ttgatttatt agcattaagg aaatcagtta
     3241 tacgttggtg tgaattgttg cttactaata agccaatggc acttataggt attattgatt
     3301 tattgaaaca ggctaaaaat aaaaaactgc aatctttaac tattgcagct gtgaatggtt
     3361 tcttcgaaga tatcatacat acaaaggtaa atgtagagga taaacaaata tatagtgatt
     3421 taaaaaatga tattgatcaa tatgcgcaaa agttgtcgtt taatcaatta attttgatgt
     3481 ttgatcaact gacggaagca cataagaaat tgaatcaaaa tgtaaatcca acgcttgtat
     3541 ttgaacaaat cgtaattaag ggtgtgagtt agatgccaaa tgtaataggt gttcagtttc
     3601 aaaaagcggg aaaattagaa tattatacac ctaatgatat acaagtagat atagaagact
     3661 gggtagttgt cgaatctaaa agaggcatag agataggtat tgttaaaaat ccattaatgg
     3721 atattgctga agaggatgtt gtgttacctc ttaaaaatat tattcgcatt gctgatgaca
     3781 aagatattga taaatttaat tgtaatgaac gagatgctga aaatgcatta atactatgta
     3841 aagacattgt aagagaacaa ggtttggaca tgcgtttagt caattgcgaa tatacattag
     3901 ataaatcgaa agttattttt aattttacgg cggatgatcg tattgatttt agaaaattag
     3961 taaaaatatt agcgcaacat ttaaaaacac gtatcgagtt gagacaaatt ggtgtaaggg
     4021 atgaagccaa attgcttggc ggtatcggac cttgtggtag gtcgttatgt tgttctacat
     4081 ttttagggga ttttgaacca gtatcgatta agatggctaa ggatcaaaat ttatcattaa
     4141 atccaactaa aatttctggt gcatgtggtc gtttgatgtg ttgtttaaaa tatgaaaatg
     4201 actattatga ggaagtacgt gcacaattac ctgatattgg tgaagcaatt gaaacgcctg
     4261 atggtaacgg gaaagtagtt gctttaaata tattagacat ttctatgcag gtgaagcttg
     4321 agggacatga acagccactt gaatataaat tagaagaaat agaaactatg cattaaggag
     4381 gcattattac atttggatcg caatgaaata tttgaaaaaa taatgcgttt agaaatgaat
     4441 gtcaatcaac tttcaaagga aacttcagaa ttaaaggcac ttgcagttga attagtagaa
     4501 gaaaatgtag cgcttcaact tgaaaatgat aatttgaaaa aggtgttggg caatgatgaa
     4561 ccaactacta ttgatactgc gaattcaaaa ccagcaaaag ctgtgaaaaa gccattacca
     4621 agtaaagata atttggctat attgtatgga gaaggatttc atatttgtaa aggcgaatta
     4681 tttggaaaac atcgacatgg tgaagattgt ctgttctgtt tagaagtttt aagtgattaa
     4741 tcaagcacac tcaaatagtg ttataattat aaatgaatat ggtttggata agtctgagac
     4801 aatgcatgtt tcaggcttta attgtgtata aagttttggt gattgcataa gagatggcgg
     4861 tactaaatgt tattattaag tgtgcacgca gtatcattag ttataaaatg tagctgttaa
     4921 aagtcaaaaa tacatcgaat gtagttaggc atataatata aaaagagttt tcaattactc
     4981 aatagaaaaa ggttgtcttc ataggagtta aaaatgttaa aagagaatga acgatttgat
     5041 caactaatca aagaagattt tagtattatt caaaatgatg atgttttttc attttcaacg
     5101 gatgctttgt tgttagggca ttttacaaaa cctagaacaa aagatattgt gttggactta
     5161 tgttcaggca atggggtgat acccttgtta ttgtttgcga aacatccacg acatatagaa
     5221 ggtgttgaga ttcaaaaaac acttgtcgat atggcgcgac gcacatttca attcaatgat
     5281 gttgatgaat atttaacaat gcatcacatg gatttgaaaa acgttactaa agtatttaaa
     5341 ccttcacaat atactttagt aacgtgtaat ccgccttatt ttaaagagaa tcagcaacac
     5401 caacatcaaa aagaagcaca taagatagcg agacatgaga ttatgtgtac acttgaagat
     5461 tgcatgattg cagcccgtca tttattaaaa gaaggtggca ggctaaacat ggtacatcgt
     5521 gcagagagac taatggatgt cttgtttgaa atgagaaaag tgaatattga acctaagaaa
     5581 gtcgttttta tatatagtaa agtagggaaa tcagcacaaa cgatagtagt agaaggtcga
     5641 aaaggtggaa atcaaggttt agaaatcatg cccccatttt atatttataa tgaagatggt
     5701 aattatagcg aagaaatgaa ggaagtatat tatggatagt cattttgtat atattgtaaa
     5761 atgtagtgat ggaagtttat atacaggata cgctaaagac gttaatgcac gtgttgaaaa
     5821 acataaccga ggtcaaggag ccaaatatac gaaagtaaga cgtccggtgc atttagttta
     5881 tcaagaaatg tatgagacaa agtctgaagc attgaagcgt gaatatgaaa ttaaaactta
     5941 taccagacaa aagaaattgc gattaattaa ggagcgatag tatggctgta ttatatttag
     6001 tgggcacacc aattggtaat ttagcagata ttacttatag agcagttgat gtattgaaac
     6061 gtgttgatat gattgcttgt gaagacacta gagtaactag taaactgtgt aatcattatg
     6121 atattccaac tccattaaag tcatatcacg aacataacaa ggataagcag actgctttta
     6181 tcattgaaca gttagaatta ggtcttgacg ttgcgctcgt atctgatgct ggattgccct
     6241 taattagtga tcctggatac gaattagtag tggcagccag agaagctaat attaaagtag
     6301 agaccgtgcc tggacctaat gctgggctga cggctttgat ggctagtgga ttaccttcat
     6361 atgtatatac atttttagga tttttgccac gaaaagagaa agaaaaaagt gctgtattag
     6421 agcaacgtat gcatgaaaat agcacattaa ttatatacga atcaccgcat cgtgtgacag
     6481 atacattaaa aacaattgca aagatagatg caacacgaca agtatcacta gggcgtgaat
     6541 taactaagaa gttcgaacaa attgtaactg atgatgtaac acaattacaa gcattgattc
     6601 agcaaggcga tgtaccattg aaaggcgaat tcgttatctt aattgaaggt gctaaagcga
     6661 acaatgagat atcgtggttt gatgatttat ctatcaatga gcatgttgat cattatattc
     6721 aaacttcaca gatgaaacca aaacaagcta ttaaaaaagt tgctgaagaa cgacaactta
     6781 aaacgaatga agtatataat atttatcatc aaataagtta atcactttat cgattatatg
     6841 aaattttaaa cgattttata aacgcaagct gtaattttaa atggtaagtt atcattttgc
     6901 attgatactg ataaaatgat gttgactatg ataaaaaaat gatgacatcg acgtttttta
     6961 atgtaaaata aatacattga aagtaataaa taccttaaca ttgaataaga tgaaaatgag
     7021 atgacgagat aaatgttcgc gtccgttgaa atgcatagaa atcttagata ttatttgaag
     7081 tgagacatta cgaggaggaa cagttatggc taaagaaaca ttttatataa caaccccaat
     7141 atactatcct agtgggaatt tacatatagg acatgcatat tctacagtgg ctggagatgt
     7201 tattgcaaga tataagagaa tgcaaggata tgatgttcgc tatttgactg gaacggatga
     7261 acacggtcaa aaaattcaag aaaaagctca aaaatctggt aagacagaaa ttgaatattt
     7321 ggatgagatg attgctggaa ttaaacaatt gtgggctaag cttgaaattt caaatgatga
     7381 ttttatcaga acaactgaag aacgtcataa acatgtcgtt gagcaagtgt ttgaacgttt
     7441 attaaagcaa ggtgatatct atttaggtga atatgaaggt tggtattctg ttccggatga
     7501 aacatactat acagagtcac aattagtaga cccacaatac gaaaacggta aaattattgg
     7561 tggcaaaagt ccagattctg gacacgaagt tgaactagtt aaagaagaaa gttatttctt
     7621 taatattagt aaatatacag accgtttatt agagttctat gaccaaaatc cagattttat
     7681 acaaccacca tcaagaaaaa atgaaatgat taacaacttc attaaaccag gacttgctga
     7741 tttagctgtt tctcgtacat catttaactg gggtgtccat gttccgtcta atccaaaaca
     7801 tgttgtttat gtttggattg atgcgttagt taactatatt tcagcattag gctatttatc
     7861 agatgatgag tcactattta acaaatactg gccagcagat attcatttaa tggctaagga
     7921 aattgtgcga ttccactcaa ttatttggcc tattttattg atggcattag acttaccgtt
     7981 acctaaaaaa gtctttgcac atggttggat tttgatgaaa gatggaaaaa tgagtaaatc
     8041 taaaggtaat gtcgtagacc ctaatatttt aattgatcgc tatggtttag atgctacacg
     8101 ttattatcta atgcgtgaat taccatttgg ttcagatggc gtatttacac ctgaagcatt
     8161 tgttgagcgt acaaatttcg atctagcaaa tgacttaggt aacttagtaa accgtacgat
     8221 ttctatggtt aataagtact ttgatggcga attaccagcg tatcaaggtc cacttcatga
     8281 attagatgaa gaaatggaag ctatggcttt agaaacagtg aaaagctaca ctgaaagcat
     8341 ggaaagtttg caattttctg tggcattatc tacggtatgg aagtttatta gtagaacgaa
     8401 taagtatatt gacgaaacaa cgccttgggt attagctaag gacgatagcc aaaaagatat
     8461 gttaggcaat gtaatggctc acttagttga aaatattcgt tatgcagctg tattattacg
     8521 tccattctta acacatgcgc cgaaagagat ttttgaacaa ttgaacatta acaatcctca
     8581 atttatggaa tttagtagtt tagagcaata tggtgtgctt aatgagtcaa ttatggttac
     8641 tgggcaacct aaacctattt tcccaagatt ggatagcgaa gcggaaattg catatatcaa
     8701 agaatcaatg caaccgcctg ctactaaaga ggaaaaagaa gagattccta gcaaacctca
     8761 aattgatatt aaagactttg ataaagttga aattaaggca gcaacgatta ttgatgctga
     8821 acatgttaag aagtcagata agcttttaaa aattcaagta gacttagatt ctgaacaaag
     8881 acaaattgta tcaggaattg ccaaattcta tacaccagat gatattattg gtaaaaaagt
     8941 agcagttgtt actaacctga aaccagctaa attaatggga caaaaatctg aaggtatgat
     9001 attatctgct gaaaaagatg gtgtattaac cttagtaagt ttaccaagtg caattccaaa
     9061 tggtgcagtg attaaataac tgtattttta aaaattagga gagataatta tgttaatcga
     9121 tacacatgtc catttaaatg atgagcaata cgatgatgat ttgagtgaag tgattacacg
     9181 tgctagagaa gcaggtgttg atcgtatgtt tgtagttggt tttaacaaat cgacaattga
     9241 acgcgcgatg aaattaatcg atgagtatga ttttttatat ggcattatcg gttggcatcc
     9301 agttgacgca attgatttta cagaagaaca cttggaatgg attgaatctt tagctcagca
     9361 tccaaaagtg attggtattg gtgaaatggg attagattat cactgggata aatctcctgc
     9421 agatgttcaa aaggaagttt ttagaaagca aattgcttta gctaagcgtt tgaagttacc
     9481 aattatcatt cataaccgtg aagcaactca agactgtatc gatatcttat tggaggagca
     9541 tgctgaagag gtaggcggga ttatgcatag ctttagtggt tctccagaaa ttgcagatat
     9601 tgtaactaat aagctgaatt tttatatttc attaggtgga cctgtgacat ttaaaaatgc
     9661 taaacagcct aaagaagttg ctaagcatgt gtcaatggag cgtttgctag ttgaaaccga
     9721 tgcaccgtat ctttcgccac atccgtatag agggaagcga aatgaaccgg cgagagtaac
     9781 tttagtagct gaacaaattg ctgaattaaa aggcttatct tatgaagaag tgtgcgaaca
     9841 aacaactaaa aatgcagaga aattgtttaa tttaaattca taaagttaaa agtgagaaag
     9901 atcaccgcca taaatgtaaa cgatgctata ttcgtttaat atgctatggt tctttctcac
     9961 ttttttaaat taaaatatcg tgcatgtgga atacgtgcga tagagatggt tagagctttg
    10021 aaattaagaa ttgtaggaag gcgttttaaa tgaaaatcaa tgagtttata gttgtagaag
    10081 gacgagatga tactgagcgt gttaaacgag ctgttgaatg tgatacgatt gaaacgaatg
    10141 gtagtgccat caacgaacaa actttagaag taattagaaa tgctcaacaa agtcgaggcg
    10201 ttattgtatt aacagatcca gatttcccag gagataaaat tagaagtaca attactgaac
    10261 atgtcaaagg tgttaaacat gcgtatattg atagagaaaa agctaaaaat aaaaaaggga
    10321 aaattggtgt tgaacatgcc gaattaattg atattaaaga agcgttaatg catgttagtt
    10381 caccctttga tgaagcttat gaatcaattg ataaatctgt gctaatagag ttggggttaa
    10441 ttgttgggaa agatgcaagg cgccgtagag aaattttaag tagaaaattg cgaatcggcc
    10501 attccaatgg taagcagtta ttgaaaaagt taaatgcatt tggttatacc gaagcggatg
    10561 taaggcaagc tttagaagat gaatgaggaa gtgaaaatgt tggataataa agatattgca
    10621 acaccatcaa gaacgcgagc gttgttagat aaatatggct ttaattttaa aaaaagttta
    10681 ggacagaact ttttgataga tgtgaatatc attaataata tcattgatgc aagtgatatt
    10741 gatgcacaaa ctggggtgat tgaaattggt ccaggcatgg ggtcattgac agaacaattg
    10801 gccagacatg ctaaaagagt attggcattt gaaattgatc aacgtttaat acctgtatta
    10861 aatgatacac tatcacctta tgataatgtg acggtgatta atgaagatat tttaaaagcg
    10921 aatattaaag aagctgttga aaatcattta caagattgtg aaaaaataat ggttgttgca
    10981 aacctgccgt actatattac gacgccaatt ttattaaatt tgatgcaaca agatatacca
    11041 attgatggct acgtggtgat gatgcaaaaa gaagtgggcg aacgcttaaa tgctgaagta
    11101 ggttcaaaag catatggttc gttatcaatt gtcgtacaat actatacaga gactagtaaa
    11161 gtattaacgg tacctaaatc tgtatttatg ccaccaccta atgttgattc aatagttgta
    11221 aaactgatgc agagaactga accgttagta acagtagata acgaggaagc attctttaag
    11281 ttagcaaaag cagcatttgc acaaagaaga aagacaatta acaataacta tcaaaattat
    11341 tttaaagatg gtaaacaaca caaagaagtg attttacaat ggttggaaca agcaggtatt
    11401 gatccaagac gtcgcggtga aacgctatct attcaagatt ttgctaaatt gtatgaagaa
    11461 aagaaaaaat tccctcaatt agaaaattaa atgattgaca aagcaaagca ctattgttaa
    11521 aatttaaatt ttgtttgacg aaaacgttgc aaatatggta ttatgtaact tgtagcgagg
    11581 tggagcaata tgccaaaatc aattttggac atcaaaaatt ctattgattg tcatgtagga
    11641 aatcgtattg tactgaaagc caatggaggc cgtaagaaaa caataaaacg ttctggaatt
    11701 ttaaaagaaa catatccgtc agttttcatt gttgagttag atcaagacaa acacaacttt
    11761 gagagagtat cttatacata cactgatgtg ttaactgaaa atgttcaagt ttcatttgaa
    11821 gaggataatc atcacgaatc aattgcacac taaataagac atatagagat gttagacgtt
    11881 tcttagtata agaagtaaat attatgataa ttatttgagt gttgggcatt atgttcaata
    11941 ctctttttat ttacaaaatg tttaacactg atgtttcgct tatagatttt tcagtaaatg
    12001 gataattgta tttataaaca caaatacaag taaatactaa gtaattagat ggagaaaatt
    12061 acttttttat taaaaaaaca ctaaaaaaca aattaaaatg tcaaatatta attctcttta
    12121 tgttaaaatc atcatattaa gataacgaaa agagggcgga aaatgatata tgaaacggca
    12181 ccagccaaaa ttaattttac gctcgataca ctttttaaaa gaaatgatgg ctatcatgag
    12241 attgaaatga taatgacaac agttgattta aatgatcgtt taacttttca taaaagaaaa
    12301 gatcgaaaga tagttgttga gattgaacat aattatgtgc cttctaatca taaaaatctc
    12361 gcatatcgtg cagcgcaact atttattgag caatatcaac taaagcaagg tgtaacaatt
    12421 tctatcgata aagaaatacc tgtttctgct ggcttagctg gaggttcggc tgatgcagca
    12481 gcaacgttaa gaggattgaa tcgacttttt gatatagggg cgagtttgga agaattggct
    12541 ctactaggca gtaaaatcgg gacagatatt ccgttttgta tttataataa aactgcacta
    12601 tgtactggaa gaggagagaa aatcgagttt ttaaataaac caccttcagc ttgggtgatt
    12661 cttgctaaac caaacttagg catatcatca ccagatatat ttaagttgat taatttagat
    12721 aagcgttacg acgtacatac gaaaatgtgt tatgaggcct tagaaaatcg agattatcaa
    12781 caattatgtc aaagtttgtc taatcgatta gagccaattt ctgtttcaaa acacccacaa
    12841 atcgataaat taaaaaataa tatgttgaaa agtggtgcag atggtgcgtt aatgagtgga
    12901 agcggaccta ctgtgtatgg gctagcacga aaagaaagcc aagcaaaaaa tatttataat
    12961 gcagttaacg gttgttgtaa tgaagtgtac ttagttagac tattaggata gaagggttga
    13021 aaagatgaga tataaacgaa gcgagagaat tgtttttatg acgcaatatt tgatgaacca
    13081 tccgaataaa ttgattccat taactttttt tgtgaaaaaa tttaaacagg cgaagtcttc
    13141 aataagtgaa gatgtccaaa ttataaaaaa tacattccaa aaagaaaagt taggtacagt
    13201 aattactact gctggcgcaa gtggtggtgt tacgtataaa ccaatgatga gtaaagaaga
    13261 ggcgactgaa gttgttaatg aggtcattac tctattagaa gagaaagaac gtttgttacc
    13321 tggcggatat ttatttttat cagatttggt aggtaatcca tcgctactaa acaaagttgg
    13381 taagttaatt gccagtattt acatggaaga aaaattagat gctgttgtta ccattgcgac
    13441 aaaaggtatt tcattggcaa atgcggttgc taatatttta aatttaccag tagtagtgat
    13501 tagaaaagac aacaaggtga ctgaaggttc tacagtttca attaattacg tttcaggatc
    13561 ttcaagaaaa atagaaacaa tggtactttc gaagagaact ttagcagaaa attcaaatgt
    13621 tttagttgtc gatgatttta tgagggctgg tggctctatt aatggtgtta tgaatttaat
    13681 gaatgagttt aaagcccatg taaaaggggt atcagtactt gtagaatcaa aagaagttaa
    13741 acaaagattg attgaagatt atacttcctt agtgaaatta tctgatgtag atgaatataa
    13801 tcaagagttt aacgtagaac ctggcaacag tttatctaag ttttcataaa aggagtttta
    13861 gtattatgaa aatcattaac acaacaagat taccggaagc acttggacca tattcgcatg
    13921 caacagttgt gaatggtatg gtttatactt ctggtcagat tccattgaat attgatggac
    13981 atatcgtaag cgctgatgtt caagcacaga caaaacaagt tttagaaaat ttaaaggttg
    14041 ttttggaaga agcaggatct gatttgaatt ctgttgcgaa agcgaccatt ttcattaaag
    14101 atatgaatga tttccaaaaa ataaatgaag tgtatggtca atattttaat gaacacaagc
    14161 cagcgcgtag ttgtgtagag gttgcgcgtt tgccaaaaga tgtgaaagta gaaattgaat
    14221 tagtaagtaa aattaaggaa ttataatttt cgattaatat gtttaatcaa gcttctaaat
    14281 aaaacagaga gatatatact ataggggggc tcactacatg aaagtgacag atgtaagact
    14341 tagaaaaata caaacagatg gacgaatgaa agcactcgtt tccattacat tagatgaagc
    14401 tttcgtaatt catgatttac gtgtaattga aggaaactct ggcttgttcg ttgcaatgcc
    14461 aagtaaacgt acaccagatg gtgaattccg cgacatcgcg catcctatta attcagatat
    14521 gagacaagaa attcaagatg cagtgatgaa agtatatgat gaaacagatg aagtagtacc
    14581 agataaaaac gctacatcag aagattcaga agaagcttaa tcaattttat atttagcgat
    14641 gtaatacatt tgcaataagt tgatttgata ctgtcgataa agcataaagc tttgtcggca
    14701 gtttttttag tttgtattaa tgttttttta tttttaatga aaggctaata aatatatacg
    14761 ttaacagatt atgatgatat gaaaattatt gattcattgc tgataaaaat tgcgtttgaa
    14821 tgatgctcgt atttttgaag taagaaaaaa gttgttttta aaattacaac gaattaaaaa
    14881 caatgccttt tatatgttga aagagtattg cagattaaat tataataatg acgaagtgta
    14941 aaatttaatg ggggttaatg ttcatgcgaa gacacgcgat aattttggca gcaggtaaag
    15001 gcacaagaat gaaatctaaa aagtataaag tgctacacga ggttgctggg aaacctatgg
    15061 tcgaacatgt attggaaagt gtgaaaggct ctggtgtcga tcaagttgta accatcgtag
    15121 gacatggtgc tgaaagtgta aaaggacatt taggcgagcg ttctttatac agttttcaag
    15181 aggaacaact cggtactgcg catgcagtgc aaatggcgaa atcacactta gaagacaagg
    15241 aaggtacgac aatcgttgta tgtggtgaca caccgctcat cacaaaggaa acattagaaa
    15301 cattgattgc gcatcacgag gatgctaatg ctcaagcaac tgtattatct gcatcgattc
    15361 aacaaccata tggatacgga agaatcgttc gaaatgcgtc aggtcgttta gaacgcatag
    15421 ttgaagagaa agatgcaacg caagctgaaa aggatattaa tgaaattagt tcaggtattt
    15481 ttgcgtttaa taataaaacg ttgtttgaaa aattaacaca agtgaaaaat gataatgcgc
    15541 aaggtgaata ttacctccct gatgtattgt cgttaatttt aaatgatggc ggcatcgtag
    15601 aagtctatcg taccaatgat gttgaagaaa tcatgggtgt aaatgatcgt gtaatgctta
    15661 gtcaggctga gaaggcgatg caacgtcgta cgaatcatta tcacatgcta aatggtgtga
    15721 caatcatcga tcctgacagc acttatattg gtccagacgt tacaattggt agtgatacag
    15781 tcattgaacc aggcgtacga attaatggtc gtacagaaat tggcgaagat gttgttattg
    15841 gtcagtactc tgaaattaac aatagtacga ttgaaaatgg tgcatgtatt caacagtctg
    15901 ttgttaatga tgctagcgta ggagcgaata ctaaggtcgg accgtttgcg caattgagac
    15961 caggcgcgca attaggtgca gatgttaagg ttggaaattt tgtagaaatt aaaaaagcag
    16021 atcttaaaga tggtgccaag gtttcacatt taagttatat tggcgatgct gtaattggcg
    16081 aacgtactaa tattggttgc ggaacgatta cagttaacta tgatggtgaa aataaattta
    16141 aaactatcgt cggcaaagat tcatttgtag gttgcaatgt taatttagta gcacctgtaa
    16201 caattggtga tgatgtattg gtggcagctg gttccacaat cacagatgac gtaccaaatg
    16261 acagtttagc tgtggcaaga gcaagacaaa caacaaaaga aggatatagg aaataatcat
    16321 ttacgtattt aaaatggcta ggataaaagg ataatcctat gtaatattaa tgtaatcttt
    16381 atgatttaat gattcgcata gtaatggagt tacattttat atataatagt aattgcgtaa
    16441 gtaaataatt ggaggactat aaatgttaaa taatgaatat aagaattcgt cattaaagat
    16501 tttttcattg aaaggaaacg aagcattagc gcaagaagtt gctgaccaag taggaattga
    16561 actaggtaaa tgttcagtta aacgttttag tgatggagaa attcaaatta atatcgaaga
    16621 gagtattcgt ggttgtgacg tatttattat tcaaccaaca tcatatcctg tgaatctaca
    16681 tttaatggaa ttattaatta tgattgatgc ttgtaaacgt gcttctgcag caacaatcaa
    16741 tattgtagtg ccatattatg gatatgcaag acaagataga aaagcccgta gccgtgagcc
    16801 aatcactgct aaattagttg caaacttaat cgaaacagct ggcgcaactc gtatgattgc
    16861 gttagactta catgcaccac aaattcaagg attctttgat attccaattg accacttaat
    16921 gggtgtgcca attcttgcta aacatttcaa agatgatccg aatattaacc cagaagaatg
    16981 tgtcgttgtt tcaccagacc atggcggcgt tacacgtgca cgtaaattag ctgacatttt
    17041 aaaaactcca attgcaatta tagataaacg tcgtcctaga ccaaatgttg ctgaagtgat
    17101 gaacattgtt ggtgagattg aaggacgtac ggcaattatt attgacgata ttattgatac
    17161 agcaggtaca atcactttag ctgcacaagc attaaaagat aaaggtgcta aagaagtata
    17221 tgcttgttgt acacaccctg ttttatcagg accggctaaa gaacgtatcg aaaattctgc
    17281 tataaaagaa ttaatcgtaa caaactcaat tcatttagat gaagatcgca aaccatctaa
    17341 cactaaagaa ttatctgttg ctggtttaat cgcacaagct atcattcgtg tatacgaaag
    17401 agaatcagtt agcgtattat ttgactaata tttaaaaggc gtttgacgaa catattccaa
    17461 acgtgtataa tagtttcgtt cgtgattata cgaataaata aacacttgca agcaacgatg
    17521 atgttgatgg gtaagtgagg tgctcgtttt gagcaaaaat gaaaggtgga aatgagaatg
    17581 gcttcattaa agtcaatcat ccgtcaaggt aaacaaacac gttcagatct taaacaatta
    17641 agaaaatctg gtaaagtacc agcagtagta tacggttacg gtactaaaaa cgtgtcagtt
    17701 aaagttgatg aagtagaatt catcaaagtt atccgtgaag taggtcgtaa cggtgttatc
    17761 gaattaggcg ttggttctaa aactatcaaa gttatggttg cagactacca attcgatcca
    17821 cttaaaaacc aaattactca cattgacttc ttagcaatca atatgagtga agaacgtact
    17881 gttgaagtac cagttcaatt agttggtgaa gcagtaggcg ctaaagaagg cggcgtagtt
    17941 gaacaaccat tattcaactt agaagtaact gctactccag acaatattcc agaagcaatc
    18001 gaagtagaca ttactgaatt aaacattaac gacagcttaa ctgttgctga tgttaaagta
    18061 actggcgact tcaaaatcga aaacgattca gctgaatcag tagtaacagt agttgctcca
    18121 actgaagaac caactgaaga agaaatcgaa gctatggaag gcgaacaaca aactgaagaa
    18181 ccagaagttg ttggcgaaag caaagaagac gaagaaaaaa ctgaagagta attttaatct
    18241 gttacattaa agtttttata ctttgtttaa caagcactgt gcttatttta atataagcat
    18301 ggtgcttttt gtgttattat aaagcttaat taaactttat tactttgtac taaagtttaa
    18361 ttaattttag tgagtaaaag acattaaact caacaatgat acatcataaa aattttaatg
    18421 tactcgattt taaaatacat acttactaag ctaaagaata atgataattg atggcaatgg
    18481 cggaaaatgg atgttgtcat tataataata aatgaaacaa ttatgttgga ggtaaacacg
    18541 catgaaatgt attgtaggtc taggtaatat aggtaaacgt tttgaactta caagacataa
    18601 tatcggcttt gaagtcgttg attatatttt agagaaaaat aatttttcat tagataaaca
    18661 aaagtttaaa ggtgcatata caattgaacg aatgaacggc gataaagtgt tatttatcga
    18721 accaatgaca atgatgaatt tgtcaggtga agcagttgca ccgattatgg attattacaa
    18781 tgttaatcca gaagatttaa ttgtcttata tgatgattta gatttagaac aaggacaagt
    18841 tcgcttaaga caaaaaggaa gtgcgggcgg tcacaatggt atgaaatcaa ttattaaaat
    18901 gcttggtaca gaccaattta aacgtattcg tattggtgtg ggaagaccaa cgaatggtat
    18961 gacggtacct gattatgttt tacaacgctt ttcaaatgat gaaatggtaa cgatggaaaa
    19021 agttatcgaa cacgcagcac gcgcaattga aaagtttgtt gaaacatcac gatttgacca
    19081 tgttatgaat gaatttaatg gtgaagtgaa ataatgacaa tattgacaac gcttataaaa
    19141 gaagataatc attttcaaga ccttaatcag gtatttggac aagcaaacac actagtaact
    19201 ggtctttccc cgtcagctaa agtgacgatg attgctgaaa aatatgcaca aagtaatcaa
    19261 cagttattat taattaccaa taatttatac caagcagata aattagaaac agatttactt
    19321 caatttatag atgctgaaga attgtataag tatcctgtgc aagatattat gaccgaagag
    19381 ttttcaacac aaagccctca actgatgagt gaacgtatta gaactttaac tgcgttagct
    19441 caaggtaaga aagggttatt tatcgttcct ttaaatggtt tgaaaaagtg gttaactcct
    19501 gttgaaatgt ggcaaaatca ccaaatgaca ttgcgtgttg gtgaggatat cgatgtggac
    19561 caatttctta acaaattagt taatatgggg tacaaacggg aatccgtggt atcgcatatt
    19621 ggtgaattct cattgcgagg aggtattatc gatatctttc cgctaattgg ggaaccaatc
    19681 agaattgagc tatttgatac cgaaattgat tctattcggg attttgatgt tgaaacgcag
    19741 cgttccaaag ataatgttga agaagtcgat atcacaactg caagtgatta tatcattact
    19801 gaagaagtga tcagccatct taaagaagag ttaaaaactg catatgaaaa tacaagaccc
    19861 aaaatagata aatcagtgcg caatgatttg aaagaaacgt atgaaagctt taaattattc
    19921 gaaagtacat actttgatca tcaaatacta cgtcgcttag tagcgtttat gtatgaaaca
    19981 ccttcgacaa ttattgagta tttccaaaaa gatgcaatca ttgcagttga tgaatttaat
    20041 cgtattaaag aaactgaaga aagtttaaca gtagagtctg attcgtttat tagcaatatt
    20101 attgaaagtg gtaatggatt tataggacaa agttttataa aatatgatga ttttgaaaca
    20161 ttgattgaag gctatcctgt cacttatttt tcattattcg ctacaacaat gccgataaaa
    20221 ctaaatcata ttattaaatt ttcatgtaaa cctgtccaac aattttatgg gcaatatgac
    20281 attatgcgtt ctgaatttca acgatatgtt aatcaaaact atcatatcgt ggttttggtc
    20341 gaaaccgaaa ctaaagttga acgtatgcaa gcgatgttaa gtgaaatgca tattccatca
    20401 ataacaaaat tgcatcgctc aatgtcatcg gggcaagcag tgattattga aggcagttta
    20461 tctgaaggat ttgaactacc tgatatggga ttagttgtca ttactgagcg tgagcttttt
    20521 aaatcaaaac agaaaaagca acgaaaacgt acgaaagcta tctcaaatgc tgaaaaaatt
    20581 aagtcttacc aagatttaaa tgtgggagat tatattgttc atgtgcatca tggtgttggt
    20641 agatatttag gtgttgagac gctcgaagtg gggcaaacgc atcgtgatta tattaaattg
    20701 caatataaag gtacggatca actatttgtt ccagtagatc aaatggatca agttcaaaaa
    20761 tatgtagctt cggaagataa gacgccaaaa ttaaataaac tcggtggcag tgaatggaaa
    20821 aaaacaaaag ctaaagttca acaaagtgtt gaagatattg ctgaagagtt gattgattta
    20881 tataaagaaa gagaaatggc agaaggttat caatatgggg aagacacagc tgagcaaaca
    20941 acatttgaat tagattttcc atatgaactt acgcctgacc aagctaaatc tatcgatgaa
    21001 attaaagatg acatgcaaaa atcgcgtcca atggatcgct tgctatgtgg tgatgttggt
    21061 tatggtaaaa ctgaagttgc agtgagagca gcattcaaag ctgtaatgga aggaaagcag
    21121 gttgcatttt tagttcctac aactatttta gctcagcaac attatgagac gttaattgag
    21181 cgtatgcaag attttcctgt tgaaattcaa ttaatgagtc gttttagaac gcctaaagag
    21241 ataaaacaaa ctaaggaagg acttaaaact ggatttgttg acatagttgt tggtacacac
    21301 aaattactta gtaaagatat acagtataaa gatttagggc tgttgattgt agatgaagaa
    21361 caacgatttg gtgtacgcca taaagagcgt attaaaacat taaaacataa tgtagatgta
    21421 ctaacattga ctgcaacccc aatacctaga acattgcata tgagtatgct aggtgtgcgg
    21481 gatttgtcag tgattgaaac gccgccagaa aatcgtttcc cagttcaaac atatgtatta
    21541 gaacagaaca tgagttttat caaagaagct ttagaaagag aactatcccg tgatggccaa
    21601 gtgttttatc tttataataa agtgcaatcc atttatgaaa aacgagaaca actccagatg
    21661 ttaatgccag atgctaacat tgcagttgct catggacaaa tgacagagcg cgatttagaa
    21721 gaaacgatgt taagttttat caataatgaa tatgatattt tagtaacgac gacgattatt
    21781 gaaacaggtg tcgatgtccc aaatgcaaat actttgatca ttgaagatgc agatcgcttt
    21841 ggattgagtc agttgtatca attaagaggt cgtgttggtc gttcaagtcg tattggttat
    21901 gcatacttct tacatccagc aaataaggta ctaactgaga ctgcagaaga tcgattacaa
    21961 gcgattaaag aatttacgga gttaggctca ggatttaaga ttgcgatgcg tgatttgaac
    22021 attcgtggtg ctggtaattt gttaggtaaa caacagcacg gctttattga tacagttgga
    22081 tttgatttgt acagtcaaat gttagaagaa gctgtaaatg aaaaacgtgg tattaaggaa
    22141 ccagaatctg aggtgccaga agtcgaagtt gatttaaact tggatgcata tttgccaaca
    22201 gaatatattg caaatgaaca agctaaaatt gaaatttata aaaagctacg aaaaactgaa
    22261 acatttgatc aaattatcga cattaaagat gaattaattg atcgtttcaa tgattatcct
    22321 gttgaagtag cacgtttgct tgatatagtg gaaataaaag tacacgcatt acattcaggt
    22381 atcacgttga ttaaagataa agggaaaata attgatattc atttatctgt aaaagccact
    22441 gaaaatattg atggcgaagt gctgttcaaa gcaacacaac ctttaggtag aacaatgaag
    22501 gttggtgttc aaaataatgc aatgacaatt actttaacga aacaaaatca atggcttgat
    22561 agtttgaagt ttttagttaa gtgcattgaa gaaagtatga gaatcagtga tgaagcataa
    22621 agaagcattt aatggcgttg tcgtgttaac tgctgcatta attgtcatta aaattctgag
    22681 tgctgtatat cgaattccat atcaaaatat attaggcgat acaggtttgt atgcatatca
    22741 acaagtgtat ccaattgtag cattaggaat gatattatcg atgaatgcca ttcctagtgc
    22801 aattacacaa aatataggga agtatcatag tgacgaagca tatgcaaaag cagtcgctta
    22861 tatacaatta gttggtatat tattatttat tgctattttt gtgtttgcga acaatattgc
    22921 acatatgatg ggtgatggcc atttaacacc aatgattcaa gctgcaagtt taagctttat
    22981 atttataggt atgcttggcg tgttaagagg ttattatcaa tctgcaaata atatgacagt
    23041 tccggctatt tcccaggtta tagaacaagt tatacgagta ggtattatca ttgttactat
    23101 tgttattttt gtagacagag gttggacgat atatgaagcg ggaacaattg ctattttagc
    23161 atcaacgata ggttttttag gttcttcaat ttatttagta gcgcaccgac cttttaagtt
    23221 taaaatggta aataacactg caaagatcgt ttggaaacag ttcgcacttt cggttttgat
    23281 tttcgctatc agtcaattaa tcgtaatttt atggcaagtg attgatagtg ttactattat
    23341 taagtcactt caagcgatac gcgtgccatt cgatgttgcc ataactgaaa aaggagtcta
    23401 tgaccgtggt gcatcattta ttcagatggg attgattgta actacaacat ttagttttgc
    23461 gctcattcct ctgttaagtg acgcaattaa aatgaataat caggtactta tgaatcgtta
    23521 tgcaaatgcg tcattaaaga ttacgatttt aataagtaca gcagcaggaa taggattaat
    23581 taatttattg cctttaatga acggtgtgtt ttttaagacg aatgatttaa ccttaacgtt
    23641 aagtgtttat atgattacgg tcatttgtgt atcgttaatt atgatggata tggcattatt
    23701 acaagcgcaa catgctgtga gacctatttt tgttggtatg acggcaggat tggttattaa
    23761 atttatactt aatatcattt tgattcgttt aagtggcatt attggtgcga gcattagtac
    23821 tgttgtatca ttaattatat tcggtacgat tatccatatt gctgtcacga gaaaatacca
    23881 cttatatgcg atgagacgat tttttatcaa tgttgtttta ggtatggtat ttatgtcgat
    23941 tgttgttcaa tgcgtgttaa acatagtgac aacacacggt agaatcactg gactcattga
    24001 attattatgt gcagcagtat taggtatcat tgcattgttt ttctatattt ttagatttaa
    24061 tgttttgaca tataaagagt taacttattt accatttggt tcaaagttgt atcaaattaa
    24121 gaaaggaaga cgttgatggc acataccatt acgattgttg gcttaggaaa ctatggcatt
    24181 gatgatttgc cgctagggat atataaattt ttaaagacac aagataaagt ttatgcaaga
    24241 acgttagatc atccagttat agaatcattg caagatgaat taacatttca gagttttgac
    24301 catgtttatg aagcacataa ccaatttgaa gatgtctata ttgatattgt ggcgcaattg
    24361 gttgaagctg ctaatgaaaa agatattgtc tatgcggttc cgggtcatcc tagagttgct
    24421 gagacaacta cagtgaaatt actggcttta gcaaaggaca atactgatat agatgtgaaa
    24481 gttttaggtg ggaaaagctt tattgatgat gtgtttgaag cagttaatgt agatccaaat
    24541 gatggcttca cactgttaga tgcgacatca ttacaagaag taacacttaa tgttagaacg
    24601 catacattga ttacgcaagt ttatagtgca atggttgctg ctaatttgaa aatcacttta
    24661 atggaacgat atcctgatga ttaccctgtt caaattgtca ctggtgcacg aagcgatggt
    24721 gcggataacg ttgtgacatg cccattatat gaattggatc atgatgaaaa tgcattcaat
    24781 aatttgacga gtgtattcgt accaaaaatc ataacatcga catatttgta tcatgacttt
    24841 gattttgcaa cggaagtgat tgatacttta gttgatgaag ataaaggttg tccatgggat
    24901 aaagtgcaaa cgcatgaaac gctaaagcgt tatttacttg aagaaacatt tgaattgttc
    24961 gaagctattg acaatgaaga tgattggcat atgattgaag aactaggaga tattttatta
    25021 caagtgttat tgcatactag tattggtaaa aaagaagggt atatcgacat taaagaagtg
    25081 attacaagtc ttaatgctaa aatgattcgt agacacccac acatatttgg tgatgccaat
    25141 gctgaaacta tcgatgactt aaaagaaatt tggtctaagg cgaaagatgc tgaaggtaaa
    25201 cagccaagag ttatatttga aaaagtattt gcagagcatt ttttaaattt atatgagaag
    25261 acgaaggata agtcatttga tgaggccgcg ttaaagcagt ggctagaaaa aggggagagt
    25321 aatacatgag attagataaa tatttaaaag tatcacggtt aataaagcga cgtacgctag
    25381 caaaagaagt aagtgatcaa ggtagaatta caataaatgg taatgttgct aaagctggat
    25441 cggatgttaa agttgaagat gtgctgacga ttcgctttgg tcaaaaatta gtaacagtta
    25501 aagtaactgc attaaatgaa catgcatcta aagataacgc gaagggtatg tatgaaatca
    25561 ttgaagagcg tcgacttgaa gaagcgtaaa ttggaggtga caagcaatga aaaataaagt
    25621 agaacatata gaaaatcagt acacgtcgca agagaacaag aaaaaacaac gtcaaaaaat
    25681 gaaaatgcgt gttgttcgta ggcgtattac agtatttgcg ggcgtattac ttgcgataat
    25741 tgttgtttta tcaatcttgc ttgttgtcca aaaacatcgc aatgatattg atgcacagga
    25801 gcgaaaagcg aaagaagcac agtttcaaaa gcaacaaaat gaagaaattg cgttaaaaga
    25861 aaagttgaat aatctgaatg acaaagatta cattgaaaaa attgcgcgtg atgattatta
    25921 cttaagcaac aaaggtgaag tgatttttag gttgccagaa gacaaagatt cgtctagctc
    25981 aaaatcttcg aaaaaataaa tccaaattga ttcaaaatta tccgagtata gacattgtga
    26041 aaaaatccaa acaaggatat aataagggaa aatcgaatca aatcgggagg atttatttaa
    26101 catatgtcaa tcgaagttgg aaataagctt aaaggtaaag tcactggtat taaaaagttt
    26161 ggtgcattcg tagaattacc tgaaggaaaa agtggtttag ttcacattag tgaagtcgca
    26221 gataattatg ttgaaaacgt agaagagcac ctttctgttg gtgatgaagt agacgtaaaa
    26281 gtattatcta ttgctgatga tggaaaaatt agtctttcaa ttaagaaagc taaagaccgt
    26341 ccacgtagac aacatacgag taaaccaagt catcaaaaac cagtgcaaaa agccgaagat
    26401 tttgaaaaga aattaagcaa tttcttaaaa gatagtgaag ataaattaac ttcaatcaaa
    26461 cgtcaaacag aatctagacg cggtggcaaa ggttcaagac gttaattaaa ataaataaag
    26521 actgcttcga taaggaatat atttagaatg atgcgtatcg aataatcgat tgcagcgtta
    26581 gacaatctaa gactgtttct taaataagga gcagtctctt ttatttgtaa tgatataact
    26641 aagacttata ccatttttga aaattgtaaa agtgaggtga tgttatgcag ttaaatagta
    26701 atggttggca tgttgatgac catattgttg tcgctgtttc tacaggtatt gatagtatgt
    26761 gtttattgta tcaactacta aatgattata aagatagtta tagaaaacta acatgcttac
    26821 atgtcaatca tggcgttagg tcagcttcaa ttgaggaagc cagattttta gaagcatact
    26881 gcgaacgtca tcacatcgat ttacatatca aaaagttaga tttgtcgcat agtctcgacc
    26941 gaaataacag cattcagaat gaagctcgaa ttaaacgtta cgaatggttt gatgaaatga
    27001 tgaatgtatt agaagcggat gtattgctaa cggcgcatca tttggacgat caattagaaa
    27061 ctattatgta tcgtattttt aatgggaaat caacacgtaa taaactagga tttgatgagt
    27121 tatcgaagcg aaaaggttat cagatttatc gaccactttt agctgtctct aaaaaagaaa
    27181 taaaacaatt ccaagagaga tatcatattc catattttga agatgaatct aataaagata
    27241 acaaatatgt tagaaatgat attcgtaata gaattattcc agctattgat gaaaataatc
    27301 aacttaaagt atcgcattta ttaaaattaa aacaatggca tgatgaacaa tatgatattt
    27361 tgcaatattc agctaaacaa tttattcaag aatttgtgaa gtttgatgaa cagtcaaaat
    27421 atttagaggt ttctagacaa gcttttaata acttaccaaa ctcattaaag atggttgtgt
    27481 tggactgcct attatcaaag tattatgagt tgtttaatat tagtgctaaa acatacgaag
    27541 agtggtttaa acaatttagt agtaagaaag cacaattcag tattaatctc acggataaat
    27601 ggataattca aatcgcatat ggtaaattaa taataatggc taaaaataat ggcgatacat
    27661 attttagagt tcaaacaatt aaaaagccag gtaattatat ttttaacaaa tatcgattag
    27721 agatacattc taatttacca aaatgtttat ttccgcttac agtgagaaca cgacaaagtg
    27781 gcgatacatt taaactgaat gggcgcgatg gttataagaa agtgaatcgc ctgtttatag
    27841 attgtaaagt gccacagtgg gttcgggatc aaatgccaat cgtattggat aaacaacagc
    27901 gcattattgc ggtaggagat ttatatcaac aacaaacaat aaaaaaatgg attataatta
    27961 gtaaaaatgg agatgaatag cgttatgcat aatgatttga aagaagtatt gttaactgaa
    28021 gaagatattc aaaatatctg taaggaattg ggagcacaat taacaaagga ttatcaaggt
    28081 aaaccattag tatgcgtggg tatcttaaaa ggctcagcaa tgtttatgtc agatttaatt
    28141 aaacgaattg atacccattt atcaattgat ttcatggatg tttctagtta tcacggaggc
    28201 actgagtcaa ctggtgaagt tcaaatcatt aaagatttag gttcttctat tgaaaataaa
    28261 gacgtattaa ttattgaaga tatcttagag actggtacta cacttaagtc aattactgaa
    28321 ttattacaat ctagaaaagt taattcatta gaaatagtta ctttattaga taaaccaaac
    28381 cgtcgtaaag cggacattga agctaagtat gtaggtaaaa aaataccaga tgaatttgtt
    28441 gttggttacg gtttagatta tcgtgaatta taccgaaact taccatatat cggtacgtta
    28501 aaacctgaag tgtattcaaa ttaatttttt aatcaatttc agttattatt actatgcgtt
    28561 tgagaaataa tagtgtagac tcaaaaatat gaaaaatgta tttcatatat atttaatttt
    28621 agacaagaca tatgtcttga aaagttgaaa aatatagaga ttgataaaac taatacgggt
    28681 gtgaatgaca ttgatgttaa gctcaattac tagcttataa aacatgtcat atgttacaat
    28741 ttttgttagt tttattatgg gaagtaggag gaaatgacgc atgcagaaag cttttcgcaa
    28801 tgtgctagtt atcgtaataa taggcgttat tatttttggt ctattttcat atttaaacgg
    28861 taatggaaat atgccgaaac agcttacata taatcaattt actgagaagt tggaaaaagg
    28921 tgaccttaaa actttagaaa tccaaccaca acaaaatgtc tatatggtaa gtggtaaaac
    28981 gaaaaatgat gaagactatt catcaactat tttatataac aacgaaaaag aattacaaaa
    29041 aattactgat gctgctaaaa agcaaaacgg tgtaaaatta acgattaaag aagaagaaaa
    29101 acaaagtgtc tttgtgagta tactttcaac attaattcca gttgtagtca tagcgttatt
    29161 atttattttc ttcctaagcc aagcacaagg tggcggtagt ggcggtcgta tgatgaactt
    29221 tggtaaatct aaagcaaaaa tgtacgataa taataaacgt cgtgttcgtt tctctgatgt
    29281 agcaggggca gatgaagaaa aacaagaatt aattgaaatt gttgatttct tgaaagataa
    29341 taaaaaattc aaagaaatgg gatctaggat tcctaaaggt gtcttacttg ttggacctcc
    29401 aggtactggt aaaacattac ttgctagagc ggttgcaggt gaagctggcg caccattctt
    29461 ctctattagt ggttcagact ttgtagagat gtttgttggt gttggtgcga gccgtgttcg
    29521 tgacttattc gataatgcta agaaaaacgc gccttgtatc atctttatcg atgagattga
    29581 tgctgttggt cgtcaacgtg gtgcaggtgt tggtggcggt catgatgaac gtgaacaaac
    29641 cctaaaccaa ttattagttg aaatggatgg tttcggtgaa aatgaaggta tcattatgat
    29701 agctgctaca aaccgtcctg atatccttga cccagcctta ttacgtccag gtcgttttga
    29761 tagacaaatt caagttggtc gtccagatgt gaaaggccgt gaagcaattc ttcatgttca
    29821 tgctaaaaac aaaccacttg atgaaacggt tgatttaaaa gcaatttcac aacgtacacc
    29881 tggtttctca ggtgctgatt tagagaactt attaaatgaa gcatctttaa ttgctgtacg
    29941 tgaaggtaaa aagaaaattg acatgagaga tatcgaagag gcaacggata gagttatagc
    30001 cggacctgct aagaaatctc gagttatttc taagaaagaa cgtaatattg ttgctcatca
    30061 cgaagctggt catacaatta tcggtatggt acttgatgag gcagaagtag tgcataaagt
    30121 tactattgtt ccacgtggac aagcaggtgg ttatgcaatg atgctaccta aacaagatcg
    30181 tttcttaatg actgaacaag agttattaga taaaatctgt ggtttacttg gtggacgtgt
    30241 atcagaagat attaacttta acgaagtatc aacaggtgct tcaaatgact tcgaacgtgc
    30301 aacacaaatc gcacgctcaa tggttacgca atatggtatg agtaaaaaat taggaccatt
    30361 acagttcggt catagcaatg gtcaagtatt cttaggtaaa gatatgcaag gtgagcctaa
    30421 ttattcaagc caaatcgcat atgaaattga taaagaagtt caacgaatcg ttaaagaaca
    30481 atacgaacgt tgtaaacaaa ttttattaga gcacaaagaa caattaattt taattgctga
    30541 aacattatta acagaagaaa cattagttgc tgaacaaatt caatcattat tctacgaagg
    30601 taaattacct gaaattgatt atgatgcagc taaagttgtt aaagatgaag attctgaatt
    30661 taatgatggt aaattcggta aatcttatga agagattcgt aaagagcaat tagaagatgg
    30721 acaacgtgac gaaagtgaag atcgtaaaga agaaaaagat attgctgagg ataaaaaaga
    30781 agctgataaa tctgatgaaa aagatgaacc agcacatcaa caagccccaa atatcgaaaa
    30841 accttacgat ccaaatcacc cagacaataa ataatcgatt atattcagta cctctttcta
    30901 tgataaagtt atagaaagag gtacttttat cgtttttgaa aatacgtatt agattttaag
    30961 tcgttgaatt gttatagcag aaaataattg taaaacaagt tacttcatta tttagaatga
    31021 tgggtgtaga ataagtacaa ttgttgcatt ttatgaagta aagtaatttt ttaaatatag
    31081 agtaatagag gagattgaaa taatgacaca cgattatatt gttaaagcat tagcatttga
    31141 tggagagatt agggcttatg ctgctttgac aactgaaact gttcaagaag cacaaacgag
    31201 acattataca tggccgacag catctgctgc aatgggaaga acaatgacag caacagctat
    31261 gatgggcgca atgttgaaag gtgatcaaaa attaactgtc actgtagatg gccaaggacc
    31321 tattggacga attattgccg atgcaaatgc taaaggcgag gtgcgtgctt atgtagacca
    31381 tccacaaact cattttccat taaatgagca aggtaaactt gatgtaagac gagcggtagg
    31441 gacaaatgga tctattatgg ttgttaaaga cgttggaatg aaagactatt tctctggagc
    31501 aagtccaatt gtttcaggag aacttggtga agattttact tattattatg ctacaagtga
    31561 acaaacacct tcatcggtag gtcttggtgt attggtaaat cctgataata cgattaaagc
    31621 agcaggagga tttatcattc aagttatgcc aggtgccaaa gatgaaacaa tttcaaaatt
    31681 agaaaaagca attagtgaaa tgacaccagt ttctaaatta attgaacaag gattaacgcc
    31741 agaaggatta ctaaacgaaa tcttaggtga agaccatgtg caaattttag agaaaatgcc
    31801 tgttcaattt gaatgtaatt gtagtcatga gaaattttta aatgctatta aaggattggg
    31861 cgaggctgag attcaaaata tgattaaaga agatcatggt gctgaagcag tatgtcattt
    31921 ctgtggaaat aaatataaat atactgaaga agaattaaac gtgttgctag aaagtttagc
    31981 gtaatttaat ttaaatcaat acgctaaaat gtttattttt agcggtttag tgaaatgtag
    32041 aactaaatag ttgtataatc cttagtgatt ttgtttgctt tctagaattt atttgataaa
    32101 ataattctat atccgataaa taaactaaga tttcaacaac taactaaaaa ggagtgttct
    32161 taatggcaca aaaaccagta gataatatta ctcaaattat tggcggtaca ccggtagtca
    32221 aattgagaaa tgtagtagat gacaatgcag cagatgttta tgtaaaattg gaatatcaaa
    32281 atccaggtgg ttctgtaaag gatagaattg ctttagcaat gattgaaaaa gcagagcgag
    32341 aaggcaaaat taaacctggc gatacaattg tagaaccaac aagtggtaat acaggtatcg
    32401 gtttagcatt tgtatgtgct gctaaaggat ataaagcagt atttactatg cccgaaacaa
    32461 tgagccaaga gcgtcgtaat ttattaaaag catacggtgc ggaattagtt ttaacgcctg
    32521 gatcagaagc gatgaaaggt gcaattaaaa aagctaaaga attgaaagaa gaacatggtt
    32581 acttcgagcc acaacaattt gaaaaccctg cgaaccctga agttcatgag ttaactacag
    32641 gtcctgagtt attacaacaa tttgaaggga aaactatcga tgcgttccta gctggtgttg
    32701 gtactggtgg tacgttatct ggtgtaggta aagttctgaa aaaagaatat cctaacatcg
    32761 aaattgttgc tatagagcct gaggcttctc cagtattgag cggtggtgag ccaggtccac
    32821 ataaattaca aggtttaggt gctggattta ttccaggcac tttgaataca gaaatctatg
    32881 acagtattat taaagtagga aatgatacag cgatggaaat gtctcgtcga gttgctaaag
    32941 aggaaggtat tttagcaggt atttcatcag gtgctgcgat ttatgctgcc attcaaaaag
    33001 caaaagaatt aggaaaaggt aaaacagtag taacagtatt gccgagtaat ggtgaacgct
    33061 acttatcaac acctttatat tcattcgatg actaattaat gtcatttaaa agagtgagtt
    33121 atctttttga gataacttgc tctttttttc taccatgtat atttttaaaa atatgagcgt
    33181 taaattaaac atttttctga taaaaatatc cagtgaatga taagataata aacgtacata
    33241 ctaataacta gtaaatagca ggagtaaatt ttattagagt taaacaatac ataattaaag
    33301 ggtggttaac atgactaaaa caaaaattat gggcatatta aacgtcacac ctgattcatt
    33361 ctcagatggt ggaaaattta ataatgttga atcagctata aatagagtga aagccatgat
    33421 agatgaaggt gctgacatta tagatgttgg aggtgtttca acgagacccg gtcatgaaat
    33481 ggtttcatta gaagaagaga tgaacagagt attacctgtt gttgaagcta ttgtcggttt
    33541 tgatgtaaaa atttcagtcg atacatttcg aagtgaggtt gctgaagcat gtttaaaatt
    33601 aggcgttgat atgattaatg atcaatgggc gggtctgtat gatcatcgta tgttccaaat
    33661 tgtagctaaa tatgacgcgg aaattatttt aatgcataat ggaaatggta atcgtgatga
    33721 accggttgtc gaagaaatgt taacatcttt gttagcacaa gcacatcaag ctaaaatagc
    33781 tggtatacct tcaaataaaa tttggctaga tccaggtata ggtttcgcta aaactagaaa
    33841 tgaagaagcc gaagttatgg caagactgga tgaacttgtt gcaacagaat atccagtttt
    33901 attagcgaca agccggaaac gtttcactaa agagatgatg ggttatgata caacaccggt
    33961 tgaaagagat gaagtaactg cagctacgac tgcatatggt attatgaaag gcgttagagc
    34021 agtacgcgtt cataatgtcg agttgaatgc taaattagct aaaggtatag attttttaaa
    34081 ggagaatgaa aatgcaagac acaatctttc ttaaaggtat gcgcttttat ggatatcatg
    34141 gtgctttatc agctgaaaat gaaatagggc aaattttcaa agtggatgta actttgaaag
    34201 tagacttagc tgaagctggg cgtactgata atgttattga tacagttcat tatggtgaag
    34261 tgttcgaaga ggttaaatca attatggaag gtaaggccgt taatttactt gagcatctag
    34321 ctgaacgtat tgcaaatcgt ataaattcac aatataatcg tgtaatggaa acgaaagtga
    34381 gaatcactaa agaaaaccca ccgattccgg gtcattatga tggagtaggt atcgaaatag
    34441 tgagggagaa taaatgattc aagcatactt aggattaggt agtaatattg gtgatagaga
    34501 aagccagtta aacgatgcta taaagatttt gaatgaatat gatggtatta acgtatctaa
    34561 tatttctccg atttatgaaa cagcaccagt tgggtatact gagcaaccta actttttaaa
    34621 tttgtgtgtt gaaattcaaa caacactcac agtattacaa ctgttggaat gttgtttgaa
    34681 gacagaagaa tgtttacacc gtattagaaa ggaacgatgg ggtcctagaa ctttagatgt
    34741 ggatattttg ttgtatggag aagaaatgat agatttacca aaactgtcgg tgccacatcc
    34801 gagaatgaat gaacgtgcat ttgttttaat cccattaaat gatatagcag caaatgtcgt
    34861 agaaccacgt tcgaaattga aagtgaaaga tttagttttt gtcgatgaca gtgtaaagag
    34921 atataaataa tgtattgttg agaacattca tatttattgg gaatagatta ttgattattg
    34981 gagatgtttc cgcattttga ttatctctaa atgcatttga tttcgaatta gtatatgatg
    35041 atttagtatg tgttagaatt aagttatttc ataaaatcaa agtaagcaat atttgataaa
    35101 ttgctgtaat ttatttaggc ttttgttgaa gtttgtggaa gataattttt atacatttac
    35161 ttagggttgt attaatgata atcgtatttt aaatgaataa gtaattcata aacgaaaaca
    35221 gaaattgctt tattacattt acaagctctt gtaaaattaa aaaagtgata aaattgatgt
    35281 tatgaatata agaaaacatg ccacatcata actttaaggt gtaatggtta atgataaagt
    35341 attagaaaca tcgaaagaga tgtatgttga gcaaaaatgt ctgatatttt ataaaacttt
    35401 aaaggaaaat gtttgagtgt accagttgga atactaaagg attacaacaa gttaaaggag
    35461 agaaagttat gtcagaagaa atgaatgacc aaatgttggt tcgacgtcaa aaattacaag
    35521 aattatatga tcttggtata gacccgtttg gttctaaatt tgaccgttca ggtttatcta
    35581 gtgatttgaa agaagagtgg gaccagtatt ctaaagaaga attggtagaa aaagaagcgg
    35641 atagtcatgt cgctatagct ggacgattaa tgactaagcg tggtaaaggt aaagcaggat
    35701 ttgcacacgt tcaggactta gctggacaaa ttcaaattta cgttcgtaaa gatcaagttg
    35761 gcgatgacga atttgattta tggaaaaatg ctgatttagg cgatatcgtt ggtgttgaag
    35821 gtgtaatgtt caaaacaaat actggcgaat tatcggttaa agcgaagaaa ttcacgctac
    35881 taactaaatc attgcgacca ttaccggata aattccacgg tttacaggat attgaacaga
    35941 gatatcgtca aagatattta gatttaatta cgaacgaaga tagcactcgt acatttatta
    36001 atcgtagtaa aatcattcaa gaaatgcgta attatttaaa taataaaggt ttcttggaag
    36061 tagaaacacc tatgatgcac caaattgctg gtggagcagc tgctagacca tttgtaacac
    36121 atcataatgc attagatgca acgttataca tgcgtattgc tattgagttg catttaaaac
    36181 gtttaattgt cggtggactt gaaaaagtat atgaaattgg tagagtattc cgtaatgaag
    36241 gtgtatcaac tagacataac cctgaattca caatgattga attatatgaa gcatatgcag
    36301 attatcatga cattatggat ttaacagaat ctatggtgag acatattgcc aatgaagtgt
    36361 taggttctgc aaaagtacaa tacaatgggg aaacgatcga tttagaatct gcttggactc
    36421 gtttgcatat tgttgatgct gtaaaagaag ctactggtgt agatttttat gaagttaaaa
    36481 gtgatgaaga agctaaagct ttagctaaag aacatggtat tgaaattaaa gatacaatga
    36541 aatatggtca tattttaaat gaattctttg agcaaaaagt tgaagaaaca cttattcagc
    36601 caacgtttat ctatggtcat ccgactgaaa tttcaccttt agcgaagaaa aatcctgaag
    36661 atcctagatt tactgatcgt ttcgaattgt tcattgtagg tagagagcat gcaaatgcat
    36721 ttactgaatt aaatgatcct attgatcaaa aaggtcgttt tgaagcgcaa cttgttgaaa
    36781 aagcgcaagg taatgatgaa gcgcatgaaa tggatgaaga ttacattgaa gcgttagaat
    36841 atggtatgcc tccgacaggt ggtcttggta tcggtattga cagattggtt atgttattaa
    36901 ctgactctcc atcaatcaga gacgtattat tattccctta tatgagacaa aaataaatga
    36961 cgttgattgt tagtaagagc tctcgtgtat acaacatgtg tatgcgaggg ctttcttaat
    37021 tatggtaatt agttcgtgtt tgaatgtttt tgatagtaat agttaacgat agtggtgcta
    37081 tttttgactg ttaaacaagg tagttggctg atagatgaaa atgacgatac gtatatagag
    37141 tagttcggat gagaaatgtt aacgatagac attgagatat cttataacaa aaattgctat
    37201 attagtataa tttatcttaa tcagctataa aagtacttta aaattgtata gaatgtgtat
    37261 ggttttgtac atatgtgtat gatagaatac taaaagtgtt atgaattaga gggcacgaga
    37321 aatgtcagtt ttgaagaata aaaaagttga ttaaaagtgt tgactttatc aattgaatga
    37381 agtaatatat aaaagtcgcc aaaaacagac gaaacacact aaaagctgat gtgacaaagt
    37441 ttacatcaaa gtgtaaaatt aactattgca ccttattaat taagcgtgta tcatgaataa
    37501 gtaagttatt ttgtctggtg actatagcaa ggaggtcaca cctgttccca tgccgaacac
    37561 agaagttaag ctccttagcg tcgatggtag tcgaacttac gttccgccag agtagaacgt
    37621 tgccaggcaa atgacaaatc ggagaattag ctcagctggg agagcatctg ccttacaagc
    37681 agagggtcgg cggttcgaac ccgtcattct ccaccattta ttcttagata tagccggcct
    37741 agctcaattg gtagagcaac tgacttgtaa tcagtaggtt gggggttcaa gtcctctggc
    37801 cggcaccatc ttttgagcca ttagctcagc tggtagagca tctgactttt aatcagaggg
    37861 tcagaggttc gaatcctcta tggctcatta cgatttaatt tttatattta gcaaaataat
    37921 gcagaagtag ttcagcggta gaatacaacc ttgccaaggt tggggtcgcg ggttcgaatc
    37981 ccgtcttctg ctccattatt ttgccggggt ggcggaactg gcagacgcac aggacttaaa
    38041 atcctgcggt gagagatcac cgtaccggtt cgattccggt cctcggcacc attttagcgc
    38101 ccgtagctca attggataga gcgtttgact acggatcaag aggttatggg ttcgactcct
    38161 atcgggcgcg ccatttttaa attaattgaa taacgggaag tagctcagct tggtagagca
    38221 cttggtttgg gaccaagggg tcgcaggttc gaatcctgtc ttcccgatta cttcttaaat
    38281 tccattttat gggggcttag ctcagctggg agagcgcctg ctttgcacgc aggaggtcag
    38341 cggttcgatc ccgctagtct ccaccattta ttttttacac gatgaacatt gaaaactgaa
    38401 tgacaatatg tcaacgttaa ttc
//
LOCUS       DAKTLH010000010        38163 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.010, whole genome shotgun sequence.
ACCESSION   DAKTLH010000010 DAKTLH010000000
VERSION     DAKTLH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 38163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.010"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            53..370
                     /locus_tag="O0394_000426"
     CDS             53..370
                     /locus_tag="O0394_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498531.1"
                     /translation="MAENIKTEQHYYTKDFSGYRNEEDNFVANQELTVTITLNEYRKL
                     IEIKAVKDKEEDTYRGKYFAEERKNEKLEKENIKLKNKIYELQNEEDNEEDEENEEEV
                     NND"
     gene            363..665
                     /locus_tag="O0394_000427"
     CDS             363..665
                     /locus_tag="O0394_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HCY0498532.1"
                     /translation="MTKKYKDMTQEEVKDLLSEKSGELYELAKEIKGESKFDILLFSS
                     IGVIDGDYLAGSSSVIGHTFDLASLLDSTKSYKDIVNVLQMCKSQKFHGIDDNKEG"
     gene            670..930
                     /locus_tag="O0394_000428"
     CDS             670..930
                     /locus_tag="O0394_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCY0498533.1"
                     /translation="MYYKFGEIKNKIISFNGFEFKVSAMKKHDGISIQIKDMNNIPLK
                     SFHVVDLSELYFATDAMRDVINEWIEENTDEQDRLINLVMKW"
     gene            944..1306
                     /locus_tag="O0394_000429"
     CDS             944..1306
                     /locus_tag="O0394_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498534.1"
                     /translation="MAILEDIFEELKLLNNNLRVLNTELSTVDSSIVQEKVKEAPMPK
                     EETAQLESIEEVKETSADLTKDYVLSVGKEFLKKADTSDKKEFRNKLNELGADKLSTI
                     KEEHYEKIVDFMNARINA"
     gene            1303..2469
                     /locus_tag="O0394_000430"
     CDS             1303..2469
                     /locus_tag="O0394_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HCY0498535.1"
                     /translation="MKLDHSNRAHAKLSASGAKQWLNCPPSIKASEGIADKSTVFAEE
                     GTFAHELSELYFSLKYEGLTQFEFNKAFQNYKRNQYYSEELREYVEEYVANVEEKYNE
                     ALSRDNDVIALFETKLDLGKYVPESFGTGDVIIFSGGVLEIIDLKYGKGIEVSAIDNP
                     QLRLYGLGAYELLSLMYDIHTIRMTIIQPRIDNFSTEELPISRLLQWGADFVKPLARL
                     AYNGGGEFKAGSHCRFCKINHSCRTRAEYMQNVPQKPPHLLSDEEIAELLYKLPDIKK
                     WADEVEQYALNQAKENDKNYPGWKLVEGRSRRMITDTKAMLEKLVEAGYKPEDITETK
                     LLSITNLEKLIGKKAFSKITEGFIEKPQGKLTLATESDKRPAIKQSAEDDFDKL"
     gene            2495..3052
                     /locus_tag="O0394_000431"
     CDS             2495..3052
                     /locus_tag="O0394_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HCY0498536.1"
                     /translation="MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQESKYSISLIIP
                     KSDTSTIKAIEQAIEAAKEEGKVSKFGGKVPANLKLPLRDGDTEREDDVNYQDAYFIN
                     ASSKQAPGIIDQNKIRLTDSGTVVSGDYIRASINLFPFNTNGNKGIAVGLNNIQLVEK
                     GEPLGGASAAEDDFDELDTDDEDFL"
     gene            3120..5072
                     /locus_tag="O0394_000432"
     CDS             3120..5072
                     /locus_tag="O0394_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HCY0498537.1"
                     /translation="MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGAISAI
                     DMTKVDNEPFHADFETFKIALFDPAVKKYAFNANFERTCLAKHFNKQMPPEEWICTMV
                     NSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLE
                     KWQQFIDYCIRDVEVEMTIAHKIKDFPVTAIEQAYWVFDQHINDRGIKLSKSLMLGAN
                     VLDKQSKEELLNQAKHITGLENPNSPTQLLAWLKDDQGLDIPNLQKKTVQEYLKEATG
                     KAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLT
                     KHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFS
                     AIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELA
                     LGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRK
                     THHTHGVRFYMKKGFLMIELPSGRALAYPKASVGENSWGSQVVEFMGLDLNRKWSKLK
                     TYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETI
                     MNKPVDWAKGLNLNSDGFTSPFYMKD"
     gene            5085..5270
                     /locus_tag="O0394_000433"
     CDS             5085..5270
                     /locus_tag="O0394_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HCY0498538.1"
                     /translation="MQHQAYINASVDIRIPTEVESVNYNQIDKEKENLADYLFNNPGE
                     LLKYNVINIKVLDLEVE"
     gene            5270..5671
                     /locus_tag="O0394_000434"
     CDS             5270..5671
                     /locus_tag="O0394_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVL family protein"
                     /protein_id="HCY0498539.1"
                     /translation="MARRKVIRVRIKGKLMTLREVSEKYHISPELLRYRYKHKMRGDE
                     LLCGRKDSKSKDEVEYMKSQIKDEEKEREKIRKKAILNLYQRNVRAEYEEERKRRLRP
                     WLYDGTPQKHSRDPYWFDVTYNQMFKKWSEA"
     gene            5671..5928
                     /locus_tag="O0394_000435"
     CDS             5671..5928
                     /locus_tag="O0394_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HCY0498540.1"
                     /translation="MSIISNRKVDMNETQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVDRVFDLWEG"
     gene            5931..6131
                     /locus_tag="O0394_000436"
     CDS             5931..6131
                     /locus_tag="O0394_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498541.1"
                     /translation="MATQKQVDYVMSLQEQLELEDCEKYTDEQVKAMSHKEVSNVIEN
                     YKTSIRNEELYYECMSFGLPNC"
     gene            6146..6388
                     /locus_tag="O0394_000437"
     CDS             6146..6388
                     /locus_tag="O0394_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498542.1"
                     /translation="MTDSARKERLNQFFGSKRYLYQDNERVAHTHVVNGTYYFHGHIV
                     PGWQSVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            6403..6651
                     /locus_tag="O0394_000438"
     CDS             6403..6651
                     /locus_tag="O0394_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCY0498543.1"
                     /translation="MNNREQIEQSIISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEDQEND"
     gene            6644..7180
                     /locus_tag="O0394_000439"
     CDS             6644..7180
                     /locus_tag="O0394_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HCY0498544.1"
                     /translation="MTNTLTIDQLQELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWF
                     NTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKNSEF
                     HNMFNFKDKEFAQDAVVSTPQIIFKEFYPDQLAIVIAIDIAFNLYSIDQLIDAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            7217..7423
                     /locus_tag="O0394_000440"
     CDS             7217..7423
                     /locus_tag="O0394_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCY0498545.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAERKY
                     EAQVKRDAVIKVGQLFENIRECGK"
     gene            7420..7623
                     /locus_tag="O0394_000441"
     CDS             7420..7623
                     /locus_tag="O0394_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498546.1"
                     /translation="MTEVKIKTISDRVYYTTTDLASDDYINLVMNLVIEDFLPVKDVF
                     NNEVWVKRDEIESFTFIKEANDD"
     gene            7616..7852
                     /locus_tag="O0394_000442"
     CDS             7616..7852
                     /locus_tag="O0394_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498547.1"
                     /translation="MINIPKMKFPKKYTEIIKKYKNKAPEEKAKIEDDFIKEIKDKDS
                     EFYSPTMANMNEYELRAMLRMMPSLIDTGDDNDD"
     gene            7845..8231
                     /locus_tag="O0394_000443"
     CDS             7845..8231
                     /locus_tag="O0394_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498548.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGIFALLLVITLPIYTVASYQHK
                     ELHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFDSADIQARLKVGDKVE
                     VKTIGYRIHFLNLYPVLYEVKKVDKQ"
     gene            8228..8380
                     /locus_tag="O0394_000444"
     CDS             8228..8380
                     /locus_tag="O0394_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0498549.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYEKI
                     RAEVSW"
     gene            8448..8648
                     /locus_tag="O0394_000445"
     CDS             8448..8648
                     /locus_tag="O0394_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCY0498550.1"
                     /translation="MWIVISIVLAIFLLILLSSISHKMKTIEALEYMNSYLFKQLVKN
                     NGVEGLEDYENEVERIRKRFKS"
     gene            8936..11383
                     /locus_tag="O0394_000446"
     CDS             8936..11383
                     /locus_tag="O0394_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HCY0498551.1"
                     /translation="MLDKVTQIETIKYDRDVSYSYAASRLSTYWTNHNMAWSDFMQKL
                     AQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGYLKEGKRRAGQVMNRSMLTLDIDYA
                     AQDMTDILSMFYDFAYCLYSTHKHREISPRLRLVIPLKRNVNADEYEAIGRKVADIVG
                     MDYFDDTTYQPHRLMYWPSTSNDAEFFFTYEDLPLLDPDKILNEYVDWTDTLEWPTSS
                     KEESKTKRLADKQGDPEEKPGIVGAFCRAYTIEEAISTFIPDLYEKHSTNRYTYHEGS
                     TAGGLVLYENNKFAYSHHNTDPVSGMLVNSFDLVRIHLYGAQDEDAKTDTPVNRLPSY
                     KAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIVNSDDAWSETLEITSKGTFKASIPNI
                     EIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTNFKTRQWQDGDDSSLRSYIEKIYDI
                     HHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGHKRLEKLFIKYLGVEDTEVNRTTTK
                     KALTAGIARVMEPGCKFDYMLTLYGPQGVGKSALLKKLGGAWFSDSLVSVTGKEAYEA
                     LQGVWLMEMAELAATRKAEVEAIKHFISKQVDRFRVAYGHYIEDFPRQCIFIGTTNKV
                     DFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEIDQIWAEAKYYYEQGEELFLNPELE
                     EEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWDDLTIFERRRFYQGDVDMLPTGNVDY
                     VERNKVCALEVFVECFGKDKGDSRGSMEIRKISNILRQLDNWSVYDGNKSGKIRFGKD
                     YGVQIAYVRDESLEDLI"
     gene            complement(11492..11590)
                     /locus_tag="O0394_000447"
     CDS             complement(11492..11590)
                     /locus_tag="O0394_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498552.1"
                     /translation="MNLMQHWKQTGLNLTARRERGVVSFVASMLHH"
     gene            11724..12014
                     /locus_tag="O0394_000448"
     CDS             11724..12014
                     /locus_tag="O0394_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCY0498553.1"
                     /translation="MKESTLEKYLVKEISKLNGLCLKWVAPGTRGVPDRIIIMPEGKT
                     YFVEMKQEKGKLHPLQKYVHRQFENRDHKVYVLWNKEQVNTFIRMVGGTFGD"
     gene            11995..13362
                     /locus_tag="O0394_000449"
     CDS             11995..13362
                     /locus_tag="O0394_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCY0498554.1"
                     /translation="MEHLAIDFKPHSYQKYAIDKVIDNEKYGLFLDMGLGKTVSTLTA
                     FSELQLLDTKKMLVIAPKQVAKDTWVDEVDKWNHLNHLKVSLVLGTPKERNDALNTEA
                     DIYVTNKENTKWLCDQYKKEWPFDMVVIDELSTFKSPKSQRFKSIKKKLPLINRFIGL
                     TGTPSPNSLQDLWAQVYLIDRGERLESSFSRYRERYFKPTHQVSEHIFKWELRDGSEE
                     KIYKQIEDICLSMKAKDYLDMPDRVDTKQTVVLSEKERKVYEELEKNYILESEEEGTV
                     VAQNGASLSQKLLQLSNGAVYTDEEDVRLIHDKKLDKLEEIIEESQGQPILLFYNFKH
                     DKERILQRFKEATTLDDSNYKERWNSGDIKLLIAHPASAGHGLNLQQGGHIIVWFGLT
                     WSLELYQQANARLYRQGQNHTTIIHHIMTDNTIDQRVYKALQNKELTQEELMKAIKAR
                     IAKHK"
     gene            13375..13812
                     /locus_tag="O0394_000450"
     CDS             13375..13812
                     /locus_tag="O0394_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0498555.1"
                     /translation="MGNTIYDIKPGTFKYIESEIYNLNENKKEIKRLRLEILNPTKEQ
                     DSNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRL
                     KYWNKEKKLKMEQIGHECHMHRNTVTTIRKNFVKAVAYHAGIK"
     gene            13969..14283
                     /locus_tag="O0394_000451"
     CDS             13969..14283
                     /locus_tag="O0394_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0498556.1"
                     /translation="MTKHNNIYKHGRKSYQYDWFYHSKAWKKLREIALDRDNHLCQMC
                     LREDIVTDANIVHHIIYVDEDFNKALELDNLMSVCYGCHNKIHANGNDKSNLKKIRVL
                     KI"
     gene            14410..14715
                     /locus_tag="O0394_000452"
     CDS             14410..14715
                     /locus_tag="O0394_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HCY0498557.1"
                     /translation="MKLTKKQLKEYIEDYKKSDDILINLYIETYEFYCRLRDELKNSD
                     LMIEHTNKAGASNIVKNPLSIELTKTVQTLNNLLKSMGLTAAQRKKIVQEEGGFGDY"
     gene            14705..16396
                     /locus_tag="O0394_000453"
     CDS             14705..16396
                     /locus_tag="O0394_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCY0498558.1"
                     /translation="MTIKVLNEPSPKLLTTWYAEQVTQGKIKTSKYVKKECERHLRYL
                     ENGGKWVFDEELAHRPIRFIEKFCKPSKGSKRQLVLQPWQHFIIGSLFGWVHKETKLR
                     RFKEALIFMGRKNGKTTTISGVANYAVSQDGENGAEIHLLANVMKQARILFDESKAMI
                     KASPKLDKNFRTLRDEIHYDATISKIMPQASDSDKLDGLNTHMGIFDEIHEFKDYKLI
                     SVIKNSRAARLQPLLIYITTAGYQLDGPLVDMVEAGRDTLDQIIEDERTFYYLASLDD
                     DDDINDSSNWIKANPNLGVSINLDEMKEEWEKAKRTPAERGDFITKRFNIFANNDEMS
                     FIDYPTLQKNNEIVSLEELEGRPCTIGYDLSETEDFTAACATFALDNGKVAVLSHSWI
                     PKHKVEYSNEKIPYREWEEDGLLTVQDKPYIDYQDVLNWIIKMNEHYVVEKITYDRAN
                     AFKLNQELKNYGFETEETRQGALTLSPALKDLKEMFLDGKIIFNNNPLMKWYINNVQL
                     KLDRNGNWLPSKQSRYRKIDGFAAFLNTYTDIMNKVVSDKGEGNIEFISIKDIMR"
     gene            16401..17639
                     /locus_tag="O0394_000454"
                     /pseudo
     CDS             16401..17639
                     /locus_tag="O0394_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500053.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            17623..18396
                     /locus_tag="O0394_000455"
     CDS             17623..18396
                     /locus_tag="O0394_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCY0498559.1"
                     /translation="MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNK
                     LDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGD
                     TIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLEKTDAVSNSAYLDKAKDLDQEHLKQ
                     MLDAETWLTAEEALSFGLIDEILGANEIAASISKEQYKRFENVPEDLKKDVDKITKID
                     DVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMNY"
     gene            18408..19571
                     /locus_tag="O0394_000456"
     CDS             18408..19571
                     /locus_tag="O0394_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCY0498560.1"
                     /translation="MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLET
                     EKAGLQQRFNIVERQVQDIEEKEKAKVKDKGEAYQSLSDNEKMVKAKAEFYRHAILPN
                     EFEKPSMEAQRLLHALPTGNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGL
                     EIPRVSYTLDDDDFITDVETAKELKAKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVN
                     WVENALQSGLAAKERKDALAVSPKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHE
                     DYRDNATIYMRYADYVKIISVLSNGTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNY
                     FGINYDGTTYDTDKDVKKGEYLFVLTAWYDQQRTLDSAFRIAKAKENTGPLPS"
     gene            19640..19918
                     /locus_tag="O0394_000457"
     CDS             19640..19918
                     /locus_tag="O0394_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCY0498561.1"
                     /translation="MSLEEIKLWLRIDYNFENDLIEGLIQSAKSELLLSGVPDYDKDD
                     LEYPLFCTAIKYIIARDYESRGYSNDQSRSKVFNEKGLQKMILKLKKW"
     gene            19930..20262
                     /locus_tag="O0394_000458"
     CDS             19930..20262
                     /locus_tag="O0394_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498562.1"
                     /translation="MEFNEFKDRAYFFQYINKGPYPDEEEKMKLYSCFCKIYNPSMKD
                     REILKATESKSGLTIIVRSSKTEYLPQTNHLVKIDSALYSDKLFNIVEIRIDTPDIGY
                     NTVVLSEK"
     gene            20259..20660
                     /locus_tag="O0394_000459"
     CDS             20259..20660
                     /locus_tag="O0394_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498563.1"
                     /translation="MSVEIKGIPEVLNKLESVYGKQAMQAKSDKALNEASEFFIKALK
                     KEFESFKDTGASIEEMTKSKPYTKVGSQERAVLIEWVGPMNRKNIIHLNEHGYTRDGK
                     KYTPRGFGVIAKTLAASERKYREIIKKELAR"
     gene            20661..21056
                     /locus_tag="O0394_000460"
     CDS             20661..21056
                     /locus_tag="O0394_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCY0498564.1"
                     /translation="MNILNTIKGILLSDAELKTHINSRIYYYKVTENAETSKPFVVIT
                     PVYDLPSDFMSDKYLSEEYLIQIDVESSNNQKTIDITKRIRYLLYQQNLIQASSQLDA
                     YFEETKRYVMSRRYQGIPKNIYYKNQRIE"
     gene            21091..21732
                     /locus_tag="O0394_000461"
     CDS             21091..21732
                     /locus_tag="O0394_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0498565.1"
                     /translation="MAEGQGSYKVGFKRLYVGVFNPEATKVVKRMTWEDEKGGTVDLN
                     ITGLAPDLVDMFASNKRVWMKKQGTNEVKSDMSIFNIPSDDLNTVIGRTKDKNGTSWV
                     GENTRAPYVTVIGESEDGLTGQPVYVALLKGTFSLDSIEFKTRGEKAEAPEPTKLTGD
                     WMNRKVDVDGTSQGIVYGYHEGKEGEAEFFKKVFVGYTDSEDHSEDSAGSLPS"
     gene            21824..22279
                     /locus_tag="O0394_000462"
     CDS             21824..22279
                     /locus_tag="O0394_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCY0498566.1"
                     /translation="MTKTLKVYKGDDVVASEQGEGKVSVTLSNLEADTTYPKGTYQVA
                     WEENGKESSKVDVPQFKTNPILVSGVSFTPETKSIMVNTDDNVEPNIAPSTATNKILK
                     YTSEHPEFVTVDENTGAIHGVAEGTSVITAMSTDGSDKSGQISVTVTNG"
     gene            22337..22687
                     /locus_tag="O0394_000463"
     CDS             22337..22687
                     /locus_tag="O0394_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498567.1"
                     /translation="MIKFEIKDRKTGKTESYTKEDVTMGEAEKCYEYLELVNQENKKE
                     APNATKMRQKERQLLVDLFKDEGLTEEDVLNKMSTKTYTKALQDIFREINGEDEEDSE
                     TEPEEMGKTEEQSQ"
     gene            22729..22887
                     /locus_tag="O0394_000464"
     CDS             22729..22887
                     /locus_tag="O0394_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498568.1"
                     /translation="MEQYGWTLTEVRKQPYVKLLEILNEENKEETEEKQSEQKVITGT
                     DLRKLFGS"
     gene            22901..29101
                     /locus_tag="O0394_000465"
     CDS             22901..29101
                     /locus_tag="O0394_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCY0498569.1"
                     /translation="MNEKVEGMTLELKLDHLGVQECMKGLKRQLGVVNSEMKANLSAF
                     DKSEKSMEKYQARIKGLNDRLKVQKKMYSQVEDELKQVNANYQKAKSSVKDVEKAYLK
                     LVEANKKEKLALDKSKEALKSSNTELKKAENQYKRTNQRKQDAYQKLKQLRDAEQKLK
                     NSNQATTAQLKRASDAVQKQSAKHKALVEQYKQEGNQVQKLKVQNDNLSKSNDKIESS
                     YAKTNTKLKQTEKEFNDLNNTIKNHSANVAKAETAVNKEKAALNNLERSIDKASSEMK
                     TFNKEQMIAQSHFGKLASQADVMSKKFSSIGDKMTSLGRTMTMGVSTPITLGLGAALK
                     TSADFEGQMSRVGAIAQASSKDLKSMSNQAVDLGAKTSKSANEVAKGMEELAALGFNA
                     KQTMEAMPGVISAAEASGAEMATTATVMASAINSFGLKASDANHVADLLARSANDSAA
                     DIQYMGDALKYAGTPAKALGVSIEDTSAAIEVLSNSGLEGSQAGTALRASFIRLANPS
                     KNTAKEMKKLGIHLSDAKGQFVGMGELIRQFQDNMKGMTREQKLATVATIVGTEAASG
                     FLALIEAGPDKINSYSKSLKNSNGESKKAADLMKDNLKGALEQLGGAFESLAIEVGKD
                     LTPMIRAGAEGLTKLVDGFTHLPGWVRKASVGLALFGAAIGPAVLAGGLLIRTVGSAA
                     KGYASLNRRIAENTILSNTNSKAMKSLGLQTLFLGSTTGKTSKGFKGLAGAMMFNLKP
                     INVLKNSAKLAILPFKLLKNGLGLAAKSLFAVSGGARFAGVALRFLTGPIGATITAIT
                     IAYKVFKTAYDRVEWFRNGINGLGETIKFFGGKIIGGAVRKLGEFKNYLGSIGKSFKE
                     KFSKDMKDGYKSLSDDDLLKVGVNKFKGFMQTMGTASKKASDTVKVLGKGVSKETEKA
                     LEKYVHYSEENSRIMEKVRLNSGQISEDKAKKLLKIETDLSNNLIAEIEKRNKKELEK
                     TQELIDKYSAFDEQEKQNILTRTKEKNDLRIKKEQELNQKIKELKEKALSDGQISENE
                     RKEIEKLENQRRDITVKELSKTEKEQERILVRMQRNRNAYSIDEASKAIKEAEKARKA
                     RKKEVDKQYEDDVIAIKNNVNLSKSEKDKLLAIADQRHKDEVRKAKSKKDAVVDVVKK
                     QNKDIDKEMDLSSGRVYKNTEKWWNGLKSWWSNFREDQKKKSDKYAKEQEETARRNRE
                     NIKKWFGNAWDGVKSKTGEAFSKMGRNANHFGGEMKKMWSGIKGIPSKLSSGWSSAKS
                     SVGYHTKAIANSTGKWFGKAWQSVKSTTGSIYNQTKQKYSDASDKAWAHSKSIWRGTS
                     KWFSNAYKSAKGWLTDMANKSRAKWDNISSTAWSNAKSVWKGTSKWFSNSYKSLKDWT
                     GDMYSRAHDRFDAISSSAWSNAKSVFNGFRKWLSKTYDWIRDIGKDMGRAAADLGKNV
                     ANKAIGGLNSMIGGINKISKAITDKNLIKPIPTLSTGTLAGKGVATDNSGALTQPTFA
                     VLNDRGSGNAPGGGVQEVIHRADGTFHAPQGRDVVVPLGVGDSVINANDTLKLQRMGV
                     LPKFHGGTKKKDWLDQLKGNIGKKAGEFGATAKNTAHNIKKGAEEMVEAAGDKIKDGA
                     SWLGDKIGDVWDYVQHPGKLVNKVMSGLNINFGGGANATVKIAKGAYSLLKKKLVDKV
                     KSWFEDFGGGGDGSYLFEYPIWQRFGRYTGGLNFNGGRHYGIDFGMPSGTNVYAVKGG
                     IADKVWTDYGGGNSIQIKTGANEWNWYMHLSKQLARQGQRIKAGQLIGKSGATGNFVR
                     GAHLHFQLMQGSHPGNDTAKDPEKWLKSLKGSGVRSGSGVNKAASAWAGDIRRAAKRM
                     GVNVTSGDVGNIISLIQHESGGNAGITQSSALRDINVLQGNPAKGLLQYIPQTFRHYA
                     VRGHNNIYSGYDQLLAFFNNRYWRSQFNPRGGWSPSGPRRYANGGLITKHQLAEVGEG
                     DKQEMVIPLTRRKRAIQLTEQVMRIIGMDGKPNNITVNNDTSTVEKLLKQIVMLSDKG
                     NKLTDALIQTVSSQDNNLGSNDAIRGLEKILSKQSGHRANANNYMGGLTN"
     gene            29101..29925
                     /locus_tag="O0394_000466"
     CDS             29101..29925
                     /locus_tag="O0394_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCY0498570.1"
                     /translation="MQSFVKIIDGYKEEVITDFNQLIFLDARAESPNTNDNSVTINGV
                     DGILPGAISFAPFSLVLRFGYDGIDVIDLNLFEHWFRSVFNRRHPYYVITSQMPGVKY
                     AVNTANVTSNLKDGSSTEIEVSLNVYKGYSESVNWTDSEFLFDSNWMFENGIPLDFTP
                     KYTHTSNQFTIWNGSTDTINPRFKHDLKILINLNASGGFELVNYTTGDIFKYNKSIDK
                     NTDFVLDGVYAYRDINRVGIDTNRGIITLAPGKNEFKIKGDVSDIKTTFKFPFIYR"
     gene            29934..31517
                     /locus_tag="O0394_000467"
     CDS             29934..31517
                     /locus_tag="O0394_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0498571.1"
                     /translation="MDYHDHLSVMDFNELICENLLDVDYGSFKEYYELNEARYITFTV
                     YRTTHNSFVFDLLICENFIIYHGEKYTIKQTAPKVEGDKVFIEVTAYHIMYEFQNHSV
                     ESNKLDDDSSETGKTPEYSLDEYLRYGFANQKTSVKMTYKIIGDFKRKVPIDELGNKN
                     GLEYCKEAVDLFGCIIYPNDTEIGFYSPETFYQRSEKVIRYQYNTDTVSATVSTLELR
                     TAIKVFGKKYTAEEKKNYNPIRTTDIKYSNGFIKEGTYRTETIGSKATINFDCKYGNE
                     TVRFTIKKGSQGGIYKLILDGKQIKQISCFAKSVQSETIDLIKNIDKGKHVLEMIFLG
                     EDPKNRIDISSNKKAKPCMYVGTEKSTVLNLIADNSGRNQYKAIVDYVADSAKQFGIR
                     YANTQTNEDIETQDKLLEFAKKQINDTPKTELDVNYIGYEKIEPRDSVFFVHELMGYN
                     TELKVVKLDRSHPFVNAIDEVSFSNEIKDMVQIQQALNRRVIAQDNRYNYQANRINHL
                     YTSTLNSPFETMDIGSVLI"
     gene            31517..31807
                     /locus_tag="O0394_000468"
     CDS             31517..31807
                     /locus_tag="O0394_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498572.1"
                     /translation="MATEEVKIKALLENDKQYFPATHWKAINGIPYAGSSDIDGLPQD
                     GIISVDDKNKLDKLKIGEAGIIQNSIVQKSPNGKLWKITVDDSGKLGTVLFY"
     gene            31823..33733
                     /locus_tag="O0394_000469"
     CDS             31823..33733
                     /locus_tag="O0394_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498573.1"
                     /translation="MENLYLIKDLGALAGRDYRAKEIQNLQRIEQFALGLTTEFKLHQ
                     KAKTMQHFAEQIYYNGRSQAAVNKSLQSQINALVVAPRNNSANEIVQARVNVNGETFD
                     TLKEHLDDWETKTQINKEETIRELNKTKQEILDIEYRFEPDKQEFLFVTELAPLTNAV
                     MQSFWFDNRTGIVYMTQARNNGYMLSRLRPNGQFIDSSLIVGGGHGTHNGYRYIDDEL
                     WIYSFILNGNNENTLVRFKYTPNVEISYGKYGMQDVFTGHPEKPYITPVINEKENKIL
                     YRIERPRSQWELENSMNYIEIRSLDDVDKNIDKVLHKISIPMRLTNETQPMQGVTFDE
                     KYLYWYTGDSNPNNRNYLTAFDLETGEEAYQVNADYGGTLDSFPGEFAEAEGLQIYYD
                     KDSGKKALMLGVTVGGDGNRTHRIFMIGQRGILEILHSRGVPFIMSDTGGRVKPLPMR
                     PDKLKNLGMLTEPGLYYLYTDHTVQIDDFPLPREWRDAGWFLEVKPPQTGGDVIQILT
                     RNSYARNMMTFERVLSGRTGDISDWNYVPKNSGKWERVPSFITKMSDINIVGMSFYLT
                     TDDTKRFTDFPTERKGVAGWNLYVEASNTGGFVHRLVRNSVTASAEILLKNYDSKTSS
                     GPWTLHEGRIIS"
     gene            33733..35199
                     /locus_tag="O0394_000470"
     CDS             33733..35199
                     /locus_tag="O0394_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HCY0498574.1"
                     /translation="MSNLEKSVAINLENTAHYENISNLDITFRTGESDSSVLLFNIIK
                     NNQPLLLSEENIKARIAIRGKGVMVVAPLEILDPFKGILKFQLPNDVIKRDGSYQAQV
                     SVAELGNSDVVVVERTITFNVEKSLFSKIPSETKLHYIVEFQELEKTIMDRAKAMDEA
                     IKNGEDYASLIEKAKEKGLSDIQIAKSSSIDELKQLANSRISDLENKAQAYSRTFDEQ
                     KRYMDEKHEAFKQSVNSGGLVTSGSTSNWQKAKITKDDGKIMQITGFDFNNPEQRIGD
                     STQFIYVSQAINYPRDVSTNGTVEYLVVTSDYKRMTYRPNGTNKVFVKRKEAGSWSEW
                     SELAINDYNTPFETVQSAQSKANMAESNAKLYADDKFNKRYSVIFDGTANGVGSTLYL
                     NESLDQFILLIFYGTFPGGDFTEFGSPFGGGKISLNPSNLPDGDGNGGGVYEFGLTKS
                     SRTSLTISNDVYFDLGSQRGSGANANRGTINKIIGVRK"
     gene            35199..35588
                     /locus_tag="O0394_000471"
     CDS             35199..35588
                     /locus_tag="O0394_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HCY0498575.1"
                     /translation="MQILVNKRNEIISYAIIGGFEEGIDIENLPENFSQVFRPKAFKY
                     SNGEIVFNEDYSEEKDDLHQQIDSEEQNTVASDDILRKMVASMQKQVVQSTKLSMQVN
                     KQNALMAKQLVTLNKKLEEVKGETENA"
     gene            35581..35745
                     /locus_tag="O0394_000472"
     CDS             35581..35745
                     /locus_tag="O0394_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCY0498576.1"
                     /translation="MLKLISPTFEDIKTWYQLKEYSKEDIAWYVDMEVIDKEEYAIIT
                     GEKYPENLES"
     gene            35791..36090
                     /locus_tag="O0394_000473"
     CDS             35791..36090
                     /locus_tag="O0394_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCY0498577.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRTQEKIYDK
                     LDRNFEELRRDKEEDEKNKEKNAKNIRDIKMWILGLIGTILSTFVIALLKTIFGI"
     gene            36226..36528
                     /locus_tag="O0394_000474"
     CDS             36226..36528
                     /locus_tag="O0394_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCY0498578.1"
                     /translation="MDAKVITRYIVLILALVNQFLANKGISPIPVDEESVSSIILTVV
                     ALYTTYKDNPTSQEGKWANQKLKKYKAESKYRKATGQAPIKEVMTPTNMNDTNDLG"
     gene            36539..37993
                     /locus_tag="O0394_000475"
     CDS             36539..37993
                     /locus_tag="O0394_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCY0498579.1"
                     /translation="MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMLATGE
                     RLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESA
                     NLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGNK
                     AKGIIKQATTKKEAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLR
                     HAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGES
                     ASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSE
                     LGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGVVASEVKAPVKNEKNPPVPAGYT
                     LDKNNVPYKKEQGNYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRW
                     ITYIANSGQRRYIATGEVDIAGNRISSFGKFSAV"
BASE COUNT        14617 a         5158 c         7710 g        10678 t
ORIGIN      
        1 tttatcaaaa tatacaactt aattaaatca aaatatacgg aggtagtcaa ctatggctga
       61 aaatattaaa actgaacaac attattacac taaagatttc tcaggataca gaaatgaaga
      121 agataacttt gtagcaaatc aagaattgac agtaacaatc acattgaacg agtacagaaa
      181 acttattgaa ataaaggctg ttaaagataa agaagaagat acttacagag gtaagtattt
      241 tgcggaagaa agaaaaaacg aaaaattgga aaaagaaaat ataaaactaa aaaacaaaat
      301 ttatgaatta caaaacgaag aagataacga ggaggacgaa gaaaacgagg aggaagtcaa
      361 caatgactaa aaaatataaa gacatgactc aggaagaagt aaaagactta ttatctgaaa
      421 aaagcggaga attgtatgaa ttagcgaaag aaattaaggg agaaagtaaa tttgatattt
      481 tgcttttctc atcaatagga gttatcgacg gagattattt agcaggttca agttctgtga
      541 ttggtcatac tttcgatctt gcttccttat tggatagcac taagagttat aaagacattg
      601 tcaatgttct ccaaatgtgt aaatcacaaa aatttcacgg tattgatgat aacaaggagg
      661 gctaaaacaa tgtattacaa atttggtgag ataaaaaata aaattatcag ctttaacggg
      721 tttgaattta aagtgtctgc gatgaaaaaa catgacggta tcagtataca aatcaaggat
      781 atgaataata ttccacttaa atcatttcat gttgtagatt taagcgaact atattttgcg
      841 acggatgcaa tgcgtgacgt tataaacgaa tggattgaag agaacacaga tgaacaggac
      901 agactaatta acttagtcat gaaatggtag gaggatttaa tcaatggcaa tattagaaga
      961 tatttttgaa gaattaaaac tattaaataa caatttacgt gtgttaaaca ctgaactatc
     1021 aacagtagat tcatcaattg tacaagagaa agttaaagaa gcaccaatgc caaaagaaga
     1081 aacagctcaa ctggaatcaa ttgaagaagt taaggaaact tctgctgatt taactaaaga
     1141 ttatgtttta tcagtaggaa aagagttcct taaaaaagca gatacttctg ataagaaaga
     1201 atttagaaat aaacttaacg aacttggtgc ggataagcta tctactatca aagaagagca
     1261 ttatgaaaaa attgttgatt ttatgaatgc gagaataaat gcatgaagct agatcactca
     1321 aatagagctc atgcaaagct tagtgcaagt ggagcaaaac aatggctaaa ctgcccaccg
     1381 agtattaagg caagtgaagg tattgcagat aaaagtacag tttttgccga agaaggtaca
     1441 ttcgcccatg agttaagtga gttatatttc agccttaaat atgaaggcct aacacagttt
     1501 gagtttaata aggcttttca aaattataag agaaatcaat attacagcga agaattgcgt
     1561 gaatatgtgg aagagtatgt agctaatgta gaagaaaaat ataacgaagc tttgagtagg
     1621 gataatgatg taatagcttt atttgaaaca aaattagatt taggtaaata cgtccctgaa
     1681 tcttttggaa ctggtgatgt cattatattt tcaggtggtg tacttgaaat tattgacctt
     1741 aaatatggta aaggcattga agtttcagct atagataatc ctcaacttag attatatggc
     1801 ttgggcgcat atgaactgct tagtttaatg tatgacattc atacaattcg catgactatc
     1861 atacaacctc gaatagataa cttttctact gaagagttac caatatcaag attacttcaa
     1921 tggggagccg attttgttaa accattagcc agacttgctt ataacggtgg aggagagttt
     1981 aaagcaggta gtcattgtag attctgtaag ataaatcatt catgtagaac acgtgcagaa
     2041 tacatgcaaa atgtgcctca aaagccacca catttgttaa gtgatgaaga gattgcagaa
     2101 cttttatata aactgcctga tatcaaaaaa tgggctgatg aagtagaaca atatgcacta
     2161 aatcaagcga aagaaaatga taaaaactat cctggttgga agcttgtaga aggtcgttca
     2221 cgaagaatga taactgatac aaaagcaatg cttgaaaagt tagttgaagc gggttataaa
     2281 cctgaagata ttacagaaac caagttactt agcattacga atttagaaaa attaattggt
     2341 aaaaaagcat tttctaaaat tacagagggt tttatagaaa agccgcaagg taaattaaca
     2401 cttgctaccg agtcggataa acgaccagct ataaagcaat ctgctgaaga tgattttgac
     2461 aaactataaa aatttaaaag gacggtatat aaacatgaaa gcaaaagtat taaataaaac
     2521 taaagtgatt acaggaaaag taagagcatc atatgcacat atttttgaac ctcacagtat
     2581 gcaagaaggg caagaatcaa agtattcaat cagtttaatc attcctaaat cagatacaag
     2641 tacgataaaa gccattgaac aagctataga agctgctaaa gaagaaggaa aagttagtaa
     2701 gtttggaggc aaagttcctg caaatctgaa acttccatta cgtgatggag atactgaaag
     2761 agaagatgat gtcaattatc aagacgctta ttttattaac gcatcaagca aacaagcacc
     2821 tggtattatt gaccaaaaca aaattagatt aacggattct ggaactgttg taagtggtga
     2881 ttatattaga gcttcaatta atctatttcc tttcaacaca aatggtaata agggtatcgc
     2941 agttggattg aacaacattc aacttgtaga aaaaggcgaa cctcttggcg gtgcaagtgc
     3001 agcagaagat gatttcgatg aattagacac tgatgatgag gatttcttat aagtcaatag
     3061 gtggggtttt agccccactt taattttaaa gaaattgagg tgtcaagaat ttgagattta
     3121 tgaatataga tattgaaaca tatagcagta atgatatttc gaaatgtggt gcctataaat
     3181 acacagaagc tgaagatttc gaaattttaa ttatagctta ttcaatagat ggtggagcga
     3241 ttagtgcgat tgacatgact aaagtagata atgagccttt ccacgctgat tttgagacgt
     3301 ttaaaattgc tctttttgat cctgctgtaa aaaagtatgc attcaatgct aatttcgaaa
     3361 gaacttgtct tgctaaacat tttaataaac agatgccacc tgaagaatgg atttgcacaa
     3421 tggttaattc aatgcgtatt ggcttacctg cttcgcttga taaagttgga gaagttttaa
     3481 gactacaaaa ccaaaaagat aaagcaggta aaaatttaat tcgttatttc tctatacctt
     3541 gtaaaccaac aaaagttaat ggaggaagaa caagaaactt gcctgaacat gatcttgaaa
     3601 aatggcaaca atttatagat tactgtattc gagatgtaga agtagaaatg acgattgctc
     3661 ataaaattaa agactttcca gtaactgcaa ttgaacaagc atattgggtt tttgaccaac
     3721 atataaacga cagaggtatt aagctttcta aatcattgat gttaggagct aatgtgctcg
     3781 ataagcagag taaagaagaa ttgcttaatc aagctaaaca tataacaggt ttagaaaatc
     3841 ctaatagtcc tacacaatta ttggcttggt taaaggatga ccaaggatta gatataccta
     3901 atttacaaaa gaaaacggtt caggagtact taaaagaagc aacaggaaaa gctaaaaaaa
     3961 tgctagaaat tagattgcaa atgtctaaaa ccagtgtgaa aaaatacaac aaaatgcatg
     4021 acatgatgtg cagtgatgaa cgggtaagag gtctgtttca attttacggt gccggtactg
     4081 gaagatgggc aggtagaggt gtacaacttc agaatttaac aaagcattat atttcagata
     4141 ctgaattaga aatagcaaga gatcttatta aagaacaacg ttttgacgat ttagatttat
     4201 tactcaatgt tcatcctcaa gacttattaa gtcaattagt taggacgaca tttactgctg
     4261 aagaaggtaa tgaactagca gtaagtgatt tttctgcaat agaggcaaga gtcatagcat
     4321 ggtatgcaaa agaacaatgg cgtttagatg tgttcaacac acacggaaag atatatgaag
     4381 catcggcttc tcaaatgttt aatgtcccgg tagaaagcat aactaaaggc gaccctctca
     4441 gacaaaaggg aaaagtgtcc gaattagctt taggttatca aggtggcgct ggagctttaa
     4501 aagcgatggg cgcattggaa atgggcattg aagaaaatga attacaaggt ttagttgata
     4561 gttggcgtaa cgcaaatcct aacatagtta atttttggaa ggcttgccaa gaggctgcaa
     4621 ttaatactgt gaaatcccga aagacgcatc atacgcatgg agttagattt tatatgaaaa
     4681 aaggttttct aatgattgaa ctgcctagtg gaagagcttt agcttatcca aaagcttcag
     4741 ttggtgaaaa tagttggggt agtcaagttg ttgaatttat gggcttagat cttaaccgta
     4801 aatggtcaaa gttaaaaacg tatggtggga agttagtcga gaatattgtt caagcaactg
     4861 caagggattt acttgcgatt tctatagcta ggcttgaagc atcaggtttt aaaatagttg
     4921 gacatgtcca tgatgaagta attgtagaaa tacctagagg ttcaaatgga cttaaggaaa
     4981 tcgaaactat catgaataag cctgtcgatt gggcaaaagg attgaatttg aatagtgacg
     5041 gatttacttc tccgttttat atgaaggatt aggagtgtga ttgaatgcaa catcaagctt
     5101 atatcaatgc ttctgttgac attagaattc ctacagaagt cgaaagtgtt aattacaatc
     5161 agattgataa agaaaaagag aatttggcgg actatttatt taataatcca ggtgaactat
     5221 taaaatataa cgttataaat atcaaggttt tagatttaga ggtggaatga tggctagaag
     5281 aaaagttata agagtgcgta tcaaaggaaa actaatgaca ttgagagaag tttcagaaaa
     5341 atatcatata tctccagaac ttcttagata cagatacaaa cataaaatgc gcggcgatga
     5401 attattgtgt ggaagaaaag actcaaaatc taaagatgaa gttgaatata tgaagagtca
     5461 aataaaagat gaagaaaaag agagagaaaa aatcagaaaa aaagcgattt tgaacctata
     5521 ccaacgaaat gtgagagcgg aatatgaaga agaaagaaag agaagattga gaccatggct
     5581 ttatgatgga acgcctcaaa aacattcacg tgatccgtac tggttcgatg tcacttataa
     5641 ccaaatgttc aagaaatgga gtgaagcata atgagcataa tcagtaacag aaaagtagat
     5701 atgaatgaaa cacaagacaa tgttaaacag cctgcgcatt acacatacgg cgacattgaa
     5761 attatagatt tcatcgaaca agttacggca cagtatccac cacaattagc attcgcaata
     5821 ggtaatgcaa tcaaatactt gtctagagca ccgttaaaga atggtcatga ggatttagca
     5881 aaggcgaagt tttacgtcga tagagtgttt gacttgtggg aggggtaacg atggcaacgc
     5941 aaaaacaagt tgattacgta atgtcattac aggagcaact ggaattagaa gactgcgaaa
     6001 aatatacaga cgaacaagtt aaagcaatga gtcataaaga agttagcaat gtgattgaga
     6061 actataagac aagcataagg aatgaagaac tatattacga atgcatgtcg tttggactgc
     6121 ctaattgtta aaaggagtga cgaccatgac agatagcgca cgtaaagaac gcttaaacca
     6181 atttttcggc tctaaaagat atctgtatca ggataacgaa cgagtggcac atactcatgt
     6241 agtaaatggc acgtattact ttcatgggca tatcgtgcca ggttggcaaa gcgttaaaaa
     6301 gacatttgat actgctgaag agctcgaaat atatataaag caacatggtt tggaatacga
     6361 ggaacagaag caactaactt tattttagag gagatggaaa tgatgaataa ccgcgaacaa
     6421 attgaacaat caattatcag tgctagtgcg tataacggca atgacacaga gggattacta
     6481 aaagagattg aagacgtgta taagaaagcg caagcgtttg atgaaatact tgaaggttta
     6541 cctaatgcta tgcaagatgc actcaaagaa gatattggtc ttgatgaagc agtagggatt
     6601 atgacggggc aagtggtcta taaatatgag gaggatcagg aaaatgacta atacattaac
     6661 aattgatcag ttacaagagt tattacaaat acaaaaggag ttcgacgata gaataccaac
     6721 gctgaactta cgagatagca aaatagcata tgtagttgaa ttctttgaat ggtttaatac
     6781 attggaaacg tttaagaact ggaagaagaa accaggtaag ccgttagatg ttcagttaga
     6841 tgaattagcg gacatgttgg cgtttggatt gagtattgcg aatcaagtag gagtgtcatc
     6901 agaagagata aaagaagcga ttgaatcaag ttttaaaaat agtgaatttc acaacatgtt
     6961 taattttaaa gataaagaat ttgctcaaga cgcagttgtt agcacaccac agataatatt
     7021 caaagaattt tatcctgacc aattggcaat tgtaatagcg atagacatag ctttcaactt
     7081 atattctatc gaccagctca ttgacgcata caaaaagaaa atgaaaagga atcatgaaag
     7141 acaagatgga acagcagacg caggaaaagg atacgtgtaa agacatctta gatcgagtca
     7201 aggaggtttt ggggaagtga cgcaatactt agtcacaaca ttcaaagatt caacaggacg
     7261 caagcataca cacataacta aagctaaaag caatcaaagg tttacagttg ttgaggcaga
     7321 gagtaaagaa gaagctgagc gcaaatacga ggcacaagtt aaaagagatg cagttattaa
     7381 agtgggtcag ttatttgaaa atataaggga gtgtgggaaa tgacggaggt taaaattaaa
     7441 actatttcag atagagttta ttacacaaca acagatctag cttctgacga ttatattaat
     7501 cttgttatga atctagtgat tgaggatttt cttccggtca aagatgtgtt caacaatgaa
     7561 gtatgggtta aaagagatga gattgaatca tttacattta ttaaggaggc aaacgatgat
     7621 taacatacct aaaatgaaat tcccgaaaaa gtacactgaa ataatcaaaa aatataaaaa
     7681 taaagcacct gaagaaaagg ctaagattga agatgatttt attaaagaaa ttaaagataa
     7741 agacagtgaa ttttacagtc ctacgatggc taatatgaat gaatatgaat taagggctat
     7801 gttaagaatg atgcctagtt taattgatac tggagatgac aatgatgatt aaaaaactta
     7861 aaaatatgga tgggttcgac atctttattg ttggaatact gtcattattc ggtatattcg
     7921 cattgctact tgttatcaca ttgcctatct atacagtggc tagttaccaa cacaaagaat
     7981 tacatcaagg aactattaca gataaatata acaagagaca agataaagaa gacaagttct
     8041 atattgtatt agacaacaaa caagtcattg aaaattccga cttattattc aaaaagaaat
     8101 ttgatagcgc agatatacaa gctaggttaa aagtaggcga taaggtagaa gttaaaacaa
     8161 tcggttatag aatacacttt ttaaatttat atccggtctt atacgaagta aagaaggtag
     8221 ataaacaatg attaaacaaa tactaagact attattctta ctagcaatgt atgagttagg
     8281 taagtatgta actgagcaag tgtatattat gatgacggct aatgatgatg tagaggcgcc
     8341 gagtgattac gaaaaaatca gagctgaagt ttcatggtaa tagctattat catttttgaa
     8401 ttaattatat taatgtgttt agcaatagca ctggaggtgt tgtaaatatg tggattgtca
     8461 tttcaatcgt tttagctata tttttattga tcttgttaag tagcatttct cataagatga
     8521 aaaccataga agcattggag tatatgaatt cttatctttt caagcagtta gtaaaaaata
     8581 atggtgttga aggtttagaa gattatgaaa atgaagttga acgaattaga aaaagattca
     8641 aaagctaaag agggggctaa agccctctcc aggattaaaa gaagcggata ttttttattt
     8701 cgtttttgta aattaaaact aatttatgtg aaattgtctt tgaagtcttg ataattaaga
     8761 aggttgaatt ttctgagatt atattagtta cgggaaaggc ttttcctgag tgcaataaaa
     8821 ttaaagttct caagttttca ttttcgtatt tatcataaat gatttcgcgt aaagttttca
     8881 aaatttgtca cctctttaat tttattaaat taattataca agaaaggagc cgaatatgtt
     8941 agacaaagtc actcaaatag aaacaattaa atatgatcgt gatgtctcat attcttatgc
     9001 tgctagtcgt ttatccacat attggactaa tcacaatatg gcttggtctg actttatgca
     9061 gaagctagca caaacagtta gaactaaaga agatttaact gagtacaata aaatgtctaa
     9121 gtctgaacaa gcagatataa aagatgttgg cggatttgtc ggtggatatt taaaagaagg
     9181 caaacgacgt gctggtcaag tcatgaatcg ttcaatgcta acacttgata tcgattatgc
     9241 tgctcaagat atgactgaca tattatctat gttttatgat tttgcatatt gtttatattc
     9301 aacacataag catagagaga taagtccaag actgcgttta gtgattcctt taaaacgaaa
     9361 tgtaaatgca gatgagtatg aagctattgg gcgtaaagtc gcagatatcg ttggcatgga
     9421 ttacttcgat gatacaactt atcaaccaca taggttaatg tattggcctt caactagcaa
     9481 tgatgcagaa tttttcttta cctatgaaga tttacctttg ttagatccag ataaaatatt
     9541 aaatgaatat gttgattgga ctgacacatt agaatggcca acgtcttcaa aggaagagag
     9601 taagactaaa agattagcag ataagcaagg tgacccagaa gaaaagccgg gaattgttgg
     9661 cgcattttgt agagcctata cgatagaaga agctatatca acttttattc ctgacttata
     9721 cgaaaaacat tctactaacc gttataccta tcatgaaggt tcaactgcag gtggattggt
     9781 gttatacgaa aataacaagt ttgcctattc tcatcataat acggatcccg ttagcggtat
     9841 gcttgtgaac agttttgatt tagtacgcat acacttatat ggtgctcaag atgaagacgc
     9901 taaaacagat actccggtta atcgactacc tagttataaa gcaatgcagc aaagagcgca
     9961 aaatgatgaa gttgttaaaa agcaattaat taacgacaaa atgtctgatg caatgcagga
    10021 tttcgatgaa atagtaaata gcgatgatgc atggtctgag acgttagaaa ttacttcgaa
    10081 aggtactttc aaagctagta tcccaaatat agaaattata ttgcgtaatg atccaaattt
    10141 aaaaggaaaa atagcattta atgaatttac aaaacaaatt gaatgcttag ggaaagtgcc
    10201 atggaatact aattttaaga cacgtcaatg gcaagacggt gatgatagca gtttaagaag
    10261 ttatatcgaa aagatttatg acatacacca ttcaggtaaa acaaaagatg ccattataag
    10321 cgtagcaatg caaaatgctt atcatccagt aagggattat ctaaataaaa tatcgtggga
    10381 tggacataaa cgtcttgaaa agttatttat caaatactta ggtgttgaag atactgaagt
    10441 gaatagaaca actaccaaaa aagcattgac tgctggaatt gctcgagtaa tggagcctgg
    10501 atgtaaattt gactatatgc ttacacttta tggtcctcaa ggtgtaggta aatctgcttt
    10561 gctaaaaaaa ttaggtggtg catggttttc tgacagttta gtttctgtta caggtaaaga
    10621 agcctatgag gccttacaag gcgtttggct aatggaaatg gcagaacttg cagctacaag
    10681 aaaagctgaa gttgaagcta ttaagcattt catatctaaa caagttgacc ggtttcgtgt
    10741 tgcttatgga cattatattg aagattttcc aaggcaatgt attttcattg gtacaactaa
    10801 taaagttgat ttcttaagag atgaaactgg tggaagacgt ttttggccaa tgactgtaaa
    10861 tccagagaga gttgaagtga actggtctaa actaaccaaa gaagagatcg accaaatttg
    10921 ggcagaagct aaatattatt atgaacaagg agaagagtta ttcctcaacc ctgaactaga
    10981 agaagaaatg cgttcaatac aaagcaaaca tactgaggaa tctccatata caggcattat
    11041 tgatgaatat cttaacacac caattcctag caattgggat gacttaacta tctttgaacg
    11101 aagacgattt tatcaaggtg atgttgatat gttaccaaca ggaaatgtag attacgttga
    11161 aagaaataag gtctgtgcgc ttgaagtgtt tgttgaatgt tttggtaaag ataagggaga
    11221 tagtagagga tctatggaaa ttagaaagat ttcaaacatc ttaagacaat tagacaattg
    11281 gtctgtatat gatggtaata aaagtgggaa aattcgattt ggaaaagatt atggtgtaca
    11341 gatagcttat gtaagagatg aaagtttaga agatttaata taataaatat tgaataaata
    11401 tacattttag agtgttgtat cagatgttgc atcatttttt gagtgatgca acacgtgagt
    11461 gtaaaaagta atcgtaggtg ttgcatcatt tttagtgatg caacattgat gcaacaaatg
    11521 atacaacacc tctttccctt ctcgctgtaa ggttcaaccc tgtttgtttc caatgttgca
    11581 tcaaattcac tataaagttt aaaaagtagt gttagggagt aaagaggtat aggggtaacc
    11641 ctctaacagc tatttttaaa agtttggcaa gaattgatgc aacatcggaa cacaaatata
    11701 aattttgtat acaaggtgaa tatatgaaag aatcgacatt agaaaaatat ttagtgaaag
    11761 agatatcaaa gctaaacggt ttatgtttaa aatgggttgc acctggaaca agaggtgtgc
    11821 cagatagaat tattattatg ccagaaggaa aaacatattt tgtagaaatg aagcaagaaa
    11881 aaggaaagtt gcatccttta caaaaatatg tgcatagaca atttgaaaat agagatcata
    11941 aagtatatgt gttatggaat aaagaacaag taaatacttt tatcagaatg gtaggtggaa
    12001 catttggcga ttgacttcaa accacatagc tatcaaaagt atgcaataga taaagtgata
    12061 gataatgaga aatacggtct gtttttagat atgggtctag ggaaaacagt atcaacactt
    12121 acagcattta gtgaattgca gttgttagac actaaaaaaa tgttagttat agcacctaaa
    12181 caagttgcta aagatacatg ggttgatgaa gttgataagt ggaaccattt aaatcatctg
    12241 aaagtgtctt tagttttagg aacacctaaa gaaagaaatg atgcattaaa cacagaggct
    12301 gatatctatg taaccaataa agaaaatact aaatggttat gtgatcaata taaaaaagaa
    12361 tggccatttg acatggttgt gattgatgaa ctgtctacat ttaaaagtcc taagagtcaa
    12421 aggtttaaat ctattaaaaa gaaattacca ctcattaata gatttatagg attaacagga
    12481 acacctagtc caaatagttt acaggattta tgggctcaag tttatttgat agacagaggt
    12541 gaaagacttg agtcttcatt cagtcgttat cgagaaaggt actttaaacc aactcatcaa
    12601 gttagcgaac atatttttaa gtgggagcta agagacggat ctgaagaaaa gatatataaa
    12661 caaatagaag atatatgttt aagcatgaaa gcgaaagatt atctggatat gcctgacaga
    12721 gttgatacta aacaaacagt agtcttatca gaaaaagaaa gaaaagtata tgaagaatta
    12781 gaaaaaaact atattttaga atcggaagaa gaaggaacag ttgtagctca aaatggggca
    12841 tcattaagtc agaaactact tcaactatct aacggcgcag tttatacaga tgaggaagat
    12901 gtaagactta tacatgataa gaagttagat aagttagagg aaattataga ggagtctcaa
    12961 ggccaaccaa tactattgtt ttataacttc aaacacgata aagaaagaat acttcaaagg
    13021 tttaaggaag caaccacatt agacgattca aactataaag aacgttggaa cagtggagac
    13081 attaagttgc ttatagcaca tccagcaagt gcaggacatg gattaaactt acaacaaggt
    13141 gggcacatta ttgtttggtt tggacttaca tggtccttgg aattatacca acaagcaaat
    13201 gctagattat acagacaagg acaaaatcat acgactatta ttcatcatat catgaccgat
    13261 aacacaatag atcaaagagt atataaagct ttacaaaata aagaactaac gcaagaagaa
    13321 ttaatgaaag ctattaaagc aagaatagct aagcataagt aatggaggtc taacatgggg
    13381 aacacaatat atgatatcaa gccaggcaca tttaaatata ttgaatcaga aatatataat
    13441 ttaaatgaga acaagaaaga aataaaaaga ttgaggttgg aaatacttaa tccaacgaag
    13501 gaacaagatt ccaatattgt atatggacca ttacaaaaag gcgaaccagt tagaacaact
    13561 gaactaatgg caactagatt attaactaat aagatgttac gaaaccttga agaaatggtc
    13621 gaagcagttg agagtgaata cttaaagttg cctgaagatc ataagaaagt aataaggctc
    13681 aagtattgga ataaagaaaa gaagttaaag atggaacaga taggacatga atgccatatg
    13741 catcgtaata ctgttactac tatacgaaag aactttgtta aagcggtagc gtatcatgca
    13801 ggtatcaaat aacattgtgc aaagattgtg caaaaggcct acaaatctgt agtaatatga
    13861 tagtatcgga aagatgtata aagttatcta aaagttatac gacacaagta cacgaggcac
    13921 atcgctatgc ggtgtgtctt ttgttatgca atcaaagagg tgtaagagat gaccaagcac
    13981 aataacattt ataagcatgg tcgtaagtca tatcaatacg attggttcta tcattcaaaa
    14041 gcatggaaga agttaaggga gatagcatta gatagagata atcatctttg tcaaatgtgt
    14101 ttacgcgaag atattgtaac agatgcaaac atagtgcatc acattattta tgttgatgaa
    14161 gattttaaca aagctttaga attagataat ctaatgtcag tttgttatgg ctgtcataac
    14221 aaaattcatg caaatggtaa tgacaaaagt aatcttaaga aaattagagt tctaaaaatt
    14281 taaataaaaa aacatttaaa taaaatttta tagccccctg cccattggct taaaatgttt
    14341 tttcgccggg taccggcggg ggcccttcgc ttgcaacgcg gataaacttt tatgaaaggg
    14401 ggtctttata tgaaattaac aaaaaaacag ctgaaagaat atatagagga ttataaaaaa
    14461 tctgatgaca tattaattaa tttgtatata gaaacgtatg aattttattg tcggttaaga
    14521 gatgaactta aaaatagtga tttgatgata gagcatacaa acaaggctgg tgcgagcaat
    14581 attgttaaga atccattaag catagaactg acaaaaacag ttcaaacact aaataactta
    14641 ctcaagtcta tgggtttaac tgcagcacaa agaaaaaaga tagttcaaga agaaggtgga
    14701 ttcggtgact attaaagttt taaatgaacc ttcaccaaaa ctattaacaa catggtatgc
    14761 agagcaagtc actcaaggga aaataaaaac aagcaaatat gttaaaaaag aatgtgagag
    14821 acaccttaga tatctagaaa atggaggtaa atgggtattt gatgaagaat tagcgcaccg
    14881 tcctattcga ttcatagaaa agttttgtaa accttccaaa ggatctaaac gtcaacttgt
    14941 attacaacca tggcaacatt ttattattgg cagtttgttt ggttgggttc ataaagaaac
    15001 aaaactgcgc aggtttaaag aagctttgat atttatgggg cgaaaaaatg gtaaaacaac
    15061 tactatatct ggtgttgcta actatgctgt ttctcaagat ggagaaaacg gcgctgaaat
    15121 ccatctttta gcaaacgtaa tgaaacaagc tagaatatta ttcgatgaat ctaaggcgat
    15181 gataaaagct agcccaaagc ttgataaaaa tttcagaaca ttaagagatg aaatccatta
    15241 tgacgcaacg atatcaaaaa ttatgcccca agcatcagat agcgataagt tagatggatt
    15301 gaatacacac atggggattt ttgatgaaat tcatgaattt aaagactata aattgatttc
    15361 agttataaaa aactcaagag ctgcaaggtt acaacctctt ctcatctaca ttacgacagc
    15421 agggtatcaa ttagatggtc cacttgttga tatggtagaa gcgggaagag acaccttaga
    15481 tcaaatcata gaagacgaaa gaacttttta ttatttagca tctttggatg atgacgatga
    15541 tattaatgat tcgtcgaact ggataaaagc aaatcccaac ttaggtgtct ctataaattt
    15601 agatgagatg aaagaagagt gggaaaaagc taagagaaca ccagctgaac gtggagattt
    15661 tataaccaaa aggtttaata tctttgctaa taatgacgag atgagtttta ttgattaccc
    15721 aacactccaa aaaaataatg aaattgtttc tttagaagag ctggaaggca gaccatgcac
    15781 gattggttat gatttatcag aaacagagga ctttacagcc gcgtgtgcta cttttgcgtt
    15841 agataatggt aaagttgcag ttttatcgca ttcatggatt cctaagcaca aagttgaata
    15901 ttctaacgaa aaaataccct atagagaatg ggaagaagat ggcttattaa cagtgcaaga
    15961 taagccttat attgactacc aagatgtttt aaattggata attaagatga atgagcatta
    16021 tgtagtagaa aaaattactt atgatagagc gaacgcattc aaactaaatc aagagttaaa
    16081 aaattacggg tttgaaacgg aagaaacaag acaaggagct ttgaccttga gccctgcatt
    16141 gaaggattta aaagaaatgt ttttagatgg gaaaataata tttaataata atcctttaat
    16201 gaaatggtat atcaataatg ttcagttgaa actagacaga aacggaaact ggttgccgtc
    16261 taagcaaagc agatatcgta aaatagatgg ctttgcagca tttttaaaca catatacaga
    16321 tattatgaat aaagttgttt ctgacaaggg tgaaggaaac atagaattta ttagtattaa
    16381 agatataatg cgttaaggag gtgaatgtta tcgcaaaaga gaatattgtc acacgcataa
    16441 agaaaaaatt gatagacaat tggattgatc agtcagcttc taagctttat gactttagcc
    16501 catggaaaaa taaatctttt tggggtgtaa tcaataatac gcttgaaact aatgaaacga
    16561 tattttcagc tattacaaag ttatctaatt cgatggctag tttgcccttg aaaatgtatg
    16621 aagattataa agtagttaat acagaagtat ctgatttact tacagtgtca ccgaataatt
    16681 ctctgagcag ttttgatttt attaatcaaa ttgaaacaat cagaaatgaa aaaggtaatg
    16741 catatgtgct aattgaacga gacatctatc atcaaccatc aaagcttttc ttattaaatc
    16801 cagatgttgt tgaaatgtta attgaaaacc aatcacgtga actttattat tccattcatg
    16861 ctgcaactgg aaataaattg attgttcata atatggacat gttgcatttt aaacacatcg
    16921 tggcatctaa tatggtgcaa ggcattagtc cgattgatgt gttgaagaat acaactgatt
    16981 ttgataatgc agtaagaacc tttaatctta cagaaatgca aaaacctgat tctttcatgc
    17041 ttaaatatgg ttccaatgta ggtaaagaaa aaaggcagca agtgttagaa gatttcaaac
    17101 agtactatga agaaaacggt ggaatattat tctaagagcc tggtgttgaa atcgaaccgt
    17161 tacctaaaaa atatgtctct gaagatatag tggcaagcga gaatttaaca agagaaagag
    17221 tagctaacgt ttttcaattg ccctcagtat tcttaaatgc aagatcaaat acaaatttcg
    17281 cgaaaaatga agagttaaac agattttact tgcagcatac cttattgcca atcgtcaaac
    17341 agtatgaaga agaatttaat cggaaactac ttactaaaac agacagagaa aaaaataggt
    17401 attttaaatt taacgttaaa tcttatttaa gggctgatag tgcaacacaa gcagaagtgt
    17461 actttaaagc agttcgtagt ggttactaca ctataaatga cattagagag tgggaagatt
    17521 taccaccagt tgaaggtgga gataagccgc taataagcgg tgatttatac ccaattgaca
    17581 cgccacttga attaagaaaa tctttgaaag gtggtgataa aaatgtcaat gaaagctaag
    17641 tattttcaaa tgaaaagaaa atcaaaaagt aaaggtgaaa tatttattta tggtgatatt
    17701 gtaagtgata aatggtttga aagtgatgta actgctacag atttcaaaaa taaactagat
    17761 gaactaggag acatcagtga aatagatgtt catataaatt catctggagg cagtgtattt
    17821 gaagggcatg caatatacaa tatgctaaaa atgcatcctg caaaaattaa tatctatgtc
    17881 gatgccttag cggcatcaat tgctagtgtt atcgctatga gtggtgacac tatttttatg
    17941 cacaaaaata gttttttaat gattcataat tcatgggtta tgactgtagg taatgcagaa
    18001 gaattaagaa agacagcgga tttacttgaa aaaacagatg ctgttagtaa ttcagcttat
    18061 ttagataaag caaaagattt agatcaagaa cacttaaaac agatgttaga tgcagaaact
    18121 tggcttactg cagaagaagc cttgtctttc ggcttgatag atgaaatttt aggagctaat
    18181 gaaatagctg ctagtatctc taaagagcaa tataagcgtt tcgagaacgt cccagaagat
    18241 ttaaagaaag atgtagacaa aatcactaaa attgatgatg tagatacatc tgaattggtt
    18301 gaaacaccta aagaaagtat gtcactagaa gaaaaagaaa aaagagaaaa aattaaacgc
    18361 gaatgcgaaa ttttaaaaat gacaatgaat tattaggagg aaatgaaatg ccgacattat
    18421 atgaattaaa acaatcctta ggtatgattg gacaacaatt aaaaaataaa aatgatgaat
    18481 tgagtcagaa agcaacagat ccaaatattg atatggaaga catcaaacaa ctagaaacag
    18541 aaaaagcagg tttacaacaa agatttaaca ttgttgaaag acaagtgcaa gacattgaag
    18601 agaaagaaaa agcgaaagtt aaagataaag gagaagctta tcaatcttta agtgataatg
    18661 agaagatggt taaagctaag gcagagtttt atcgtcacgc gattttacca aatgaatttg
    18721 aaaaaccttc aatggaggca caacgtttat tacacgcttt accaacagga aatgattcag
    18781 gtggagataa gctcttacca aaaacacttt ctaaagaaat tgtttcagaa ccatttgcta
    18841 aaaaccaatt acgtgaaaaa gctcgtctaa ctaacattaa aggtttagag attccaagag
    18901 tttcatacac tttagacgat gatgatttca ttacagacgt agaaacagca aaagaattaa
    18961 aagcaaaagg tgatacagtc aagttcacta ctaataaatt caaagtattt gctgcaattt
    19021 cagatactgt aattcatgga tcagatgtag atttagtaaa ctgggttgaa aacgcactac
    19081 aatcaggatt agcagctaaa gagcgtaaag atgccttagc agtaagtcct aaatctggat
    19141 tagaacacat gtcattttat aatggatctg ttaaagaagt tgagggagca gacatgtatg
    19201 atgctattat taacgcttta gcagatttac atgaagatta tcgtgataac gcaacaattt
    19261 atatgcgata tgcagattat gtcaaaatta ttagtgttct ttcaaatgga acaacaaatt
    19321 tctttgacac accagcagaa aaagtatttg gcaaaccagt agtatttaca gatgcagcag
    19381 ttaaacctat tgtgggagat ttcaattatt ttggaattaa ctatgatgga acaacttatg
    19441 acactgataa agatgttaaa aaaggcgaat atttgtttgt attaacagca tggtatgatc
    19501 agcaacgtac attagacagt gcattcagaa ttgcaaaagc aaaagaaaat acaggtccat
    19561 tacccagcta agccccaaaa ggttaatgta acagctaagg ctaaatcagc tgtaatatca
    19621 gccgaatagg ggtgatgaaa tgagtttaga agaaattaaa ttgtggttga gaattgacta
    19681 taatttcgaa aatgatttaa ttgaaggtct cattcaatcg gctaagtctg aattactatt
    19741 aagtggggtt ccagattatg acaaagatga cttggaatac ccgctttttt gtacagcgat
    19801 taaatatatc attgcaagag attatgaaag tcgtggatac tcaaatgacc aatctagaag
    19861 caaggtgttt aatgaaaaag gattgcaaaa aatgattttg aaattaaaaa agtggtaggt
    19921 gatttttaaa tggaatttaa tgaatttaaa gatcgcgcgt atttttttca atatataaac
    19981 aaaggaccat atccagatga agaggaaaaa atgaaattgt atagttgctt ttgtaaaatt
    20041 tataatcctt ctatgaaaga tagagaaatt ttaaaagcga ctgaatcaaa atcaggatta
    20101 accataattg tcaggtcttc taaaactgaa tatctaccac aaacaaatca cttagttaaa
    20161 attgacagtg cattatattc cgataaatta ttcaacattg tagaaataag aattgataca
    20221 ccagatattg gctataatac agtggtttta tcagaaaaat gagtgtagaa attaaaggga
    20281 tacctgaagt gttgaataaa ttagaatcgg tatacggtaa acaagcaatg caggctaaga
    20341 gtgataaagc tttaaatgaa gcatctgaat tttttataaa ggctttaaag aaagagttcg
    20401 agagctttaa agatacgggt gccagtatag aagaaatgac taaatctaag ccttatacaa
    20461 aagttggcag tcaagaaagg gctgttttaa ttgaatgggt aggccctatg aatcgcaaaa
    20521 acattattca cttgaatgaa catggttata caagagatgg aaaaaaatat acaccaagag
    20581 gttttggagt tattgcaaaa acattagctg ctagcgaacg taagtataga gaaattataa
    20641 aaaaggagtt ggccagataa atgaatatat taaacaccat aaaaggaatt ttattatctg
    20701 atgcagagct caaaacacat ataaattcta gaatatacta ttacaaagtc actgaaaacg
    20761 ctgaaacttc caaacccttt gttgttatta cacctgttta tgatttgcct tcagacttta
    20821 tgtctgataa atatctcagt gaagaatact taattcaaat agatgtagaa tcttcaaata
    20881 atcagaaaac aattgatata acaaaacgaa taagatacct gttatatcaa caaaatttaa
    20941 ttcaagcatc tagtcagtta gatgcttatt ttgaagaaac taaacgttat gtgatgtcga
    21001 gacgatatca aggcataccc aaaaatatat attataaaaa tcagcgcatc gaataggtgt
    21061 gctttttaat ttttaaggag gaaataagca atggcagaag gacaaggttc ttataaagta
    21121 ggttttaaaa gattatacgt tggagttttt aacccagaag caacaaaagt agttaaacgc
    21181 atgacatggg aagatgaaaa aggtggtaca gttgacctaa atatcacagg tttagcacca
    21241 gatttagtag atatgtttgc atctaacaaa cgtgtatgga tgaaaaaaca aggtactaat
    21301 gaagttaagt ctgacatgag tattttcaat attccaagtg atgatttaaa cacagttatt
    21361 ggacgtacta aagataaaaa tggtacatct tgggtaggag agaatacaag agcaccgtat
    21421 gtaacagtaa ttggcgaatc ggaagatggt ttaacaggtc agccggtata tgtagcctta
    21481 cttaaaggta cttttagttt agattcaatt gaatttaaaa cacgaggtga aaaagcagaa
    21541 gccccagaac ctacaaaatt aacaggtgac tggatgaata gaaaagttga tgttgatgga
    21601 acgtcacaag gtattgtata cggttatcat gaaggtaaag aaggagaagc agaattcttc
    21661 aaaaaagtat tcgttggata cacggacagt gaagatcatt cagaggattc tgcaggttcg
    21721 ttacccagct aatccccaaa atgttgaagt agcagttaat tcaaaatctg caacagtttc
    21781 agcagaatag gggctttcaa aataaatcaa aggagaataa tttatgacta aaactttaaa
    21841 ggtttataaa ggagacgacg tcgtagcttc tgaacaaggt gaaggcaaag tgtcagtaac
    21901 tttatctaat ttagaagcgg atacaactta tccaaaaggt acttaccaag tggcatggga
    21961 agaaaatggt aaagaatcta gtaaagttga tgtacctcaa ttcaaaacca atccaattct
    22021 agtctcaggc gtatcattta caccagaaac taaatcaatt atggtaaata ccgatgacaa
    22081 tgttgagcca aacattgcac caagcacagc aacgaataaa atattgaaat atacaagtga
    22141 acatccagaa tttgttactg tagatgaaaa tacaggagca attcacggtg tagctgaagg
    22201 tacttcagta atcactgcta tgtctactga tggaagcgat aagtcaggac aaatttcagt
    22261 gacagtaaca aacggatagg gatttaaggc gcagtatatc tgcgtctttt ttatttgaat
    22321 aaaaggagct aatacaatga ttaaatttga aattaaagat cgtaaaacag gaaaaacaga
    22381 gagctataca aaagaagatg taacaatggg cgaagcagaa aaatgctatg agtatttaga
    22441 attagtaaat caagagaata aaaaagaagc acctaacgca acaaaaatga gacaaaaaga
    22501 gcgacagtta ttagtagatt tatttaaaga tgaaggattg actgaagaag atgttctgaa
    22561 caagatgagt actaaaactt atacaaaagc cttacaagat atatttcgag aaatcaatgg
    22621 tgaagatgaa gaagattcag aaactgaacc agaagagatg ggaaagacag aagaacaatc
    22681 tcaataaaag acattttatc gaacattaag aaaatacaac gtttctgtat ggagcagtat
    22741 gggtggacat taactgaagt cagaaaacag ccgtatgtaa aacttttaga aatacttaat
    22801 gaagagaata aagaagagac tgaagaaaaa caaagtgaac aaaaagtcat tacaggtacg
    22861 gatttaagaa aactttttgg aagctagaaa ggaggttaat atgaatgaaa aagtagaagg
    22921 catgaccttg gagctgaaat tagaccattt aggtgtccaa gaatgcatga aaggtttaaa
    22981 gcgacaatta ggtgttgtta atagtgaaat gaaagctaat ctgtcagcat ttgataagtc
    23041 tgaaaaatca atggaaaaat atcaggcgag aattaagggg ttaaatgata ggcttaaagt
    23101 tcaaaaaaag atgtattctc aagtagaaga tgagcttaaa caagttaacg ctaattacca
    23161 aaaagctaaa tccagtgtaa aagatgttga gaaagcatat ttaaagttag tagaagccaa
    23221 taaaaaagaa aaattagctc ttgataaatc taaagaagcc ttaaaatcat cgaatacaga
    23281 acttaaaaaa gctgaaaatc aatataaacg tacaaatcaa cgtaaacaag atgcgtatca
    23341 aaaacttaaa cagttgagag atgcagaaca aaagcttaag aatagtaacc aagctactac
    23401 tgcacaacta aaaagagcaa gtgacgcagt acagaagcag tccgctaagc ataaagcact
    23461 tgttgaacaa tataaacaag aaggcaatca agttcaaaaa ctaaaagtgc aaaatgacaa
    23521 tctttcaaaa tcaaatgata aaattgaaag ttcttacgct aaaactaata ctaaattaaa
    23581 gcaaacagaa aaagaattta atgatttaaa caatactatt aagaatcata gcgctaatgt
    23641 cgcaaaagct gaaacagctg ttaataaaga aaaagctgct ttaaataatt tggagcgttc
    23701 aatagataaa gcttcatccg aaatgaagac ttttaacaaa gaacaaatga tagctcaaag
    23761 tcatttcggt aaacttgcaa gtcaagcgga tgtcatgtca aagaaattta gttctattgg
    23821 agacaaaatg acttccctgg gacgtacaat gacgatgggc gtatctacac caattacttt
    23881 agggttaggt gcagcattaa aaacaagtgc agactttgaa ggccaaatgt ctcgagttgg
    23941 agcgattgcg caagcaagca gtaaagactt gaaaagcatg tctaatcaag cagttgactt
    24001 aggagctaaa accagtaaaa gtgctaacga agttgctaaa ggtatggaag aattggcagc
    24061 tttaggcttt aatgccaaac aaacaatgga ggctatgcca ggtgttatca gtgcagcaga
    24121 agcaagtggt gcagaaatgg ctacaactgc aactgtaatg gcttcagcga ttaactcttt
    24181 cggtttaaaa gcatctgatg caaatcatgt tgctgattta cttgcgagat cagcaaatga
    24241 tagtgctgca gatattcagt acatgggaga tgcattgaag tatgctggta ctcctgcaaa
    24301 agcattagga gtttcaatag aggacacttc cgcagcaatt gaagttttat ctaactcagg
    24361 tttagagggt tctcaagcag gtactgccct aagagcttca tttatcaggc tagctaatcc
    24421 aagtaaaaat acagctaagg aaatgaaaaa attaggtatt catttgtctg atgctaaagg
    24481 tcaatttgtt ggcatgggtg aattgattag acagttccaa gataatatga aaggcatgac
    24541 gagagaacaa aaactagcta cagtggctac aatagttggt actgaagcag caagtggatt
    24601 tttagccttg attgaagcgg gaccagataa aattaatagc tatagtaaat ccttaaagaa
    24661 ttccaatggc gaaagtaaaa aagcagcaga tttgatgaaa gataatctca aaggcgctct
    24721 ggaacaatta ggtggcgctt ttgaatcatt agcaatcgaa gtcggtaaag atttaacgcc
    24781 tatgattaga gcaggagcgg aaggtttaac aaaattagtt gatggattta cacatctccc
    24841 tggttgggtt agaaaagctt cagtaggatt agcacttttt ggtgcagcaa ttggacctgc
    24901 agttcttgct ggagggttat taatacgtac agttggaagt gctgctaaag gatatgcgtc
    24961 attaaataga cgtattgctg aaaatacaat cctttcaaat actaattcaa aagcaatgaa
    25021 atctttaggt cttcaaacat tatttcttgg ttctacaaca ggaaaaacgt caaaaggctt
    25081 taaagggtta gccggagcta tgatgtttaa tttaaaacct ataaatgttt tgaaaaattc
    25141 tgcaaagcta gcaattttac cgttcaaact tttgaaaaac ggtttaggat tagctgcaaa
    25201 atctttattt gcagtaagtg gaggcgcaag atttgcgggt gtagccttaa ggtttttaac
    25261 aggacctata ggtgctacaa taactgctat tacaattgcg tataaagttt ttaaaaccgc
    25321 atatgatcgt gtggaatggt tcagaaacgg tattaacggt ttaggagaaa ctataaagtt
    25381 ttttggtggt aaaattattg gcggcgctgt tagaaagcta ggagagttta aaaactatct
    25441 tggaagtatc ggcaaaagct tcaaagaaaa gttttcaaaa gatatgaaag atggttataa
    25501 atcattaagc gacgatgacc ttctcaaagt aggagtcaac aagtttaaag gatttatgca
    25561 aaccatgggc acagcttcta aaaaagcgtc tgatactgta aaagtgttag ggaaaggtgt
    25621 ttcaaaagaa acagaaaaag ctttagaaaa atatgtgcat tattctgaag aaaatagcag
    25681 aatcatggaa aaagtacgtt taaactcggg tcagatatca gaagacaaag caaaaaaact
    25741 tttgaaaatt gaaacggatt tatctaataa ccttatagct gaaatagaaa aaagaaataa
    25801 aaaggaactc gaaaaaactc aagaacttat tgataagtat agtgcattcg atgaacaaga
    25861 aaagcaaaac attttaactc gaactaaaga aaaaaatgac ttgcgaatta aaaaagagca
    25921 agaactcaat cagaaaatca aagaattgaa agaaaaagct ttgagtgatg gtcagatttc
    25981 agaaaatgaa agaaaagaaa ttgaaaagct tgaaaatcaa agacgtgata tcactgttaa
    26041 agaattgagt aagactgaaa aagagcaaga gcgtatttta gtaagaatgc aaagaaacag
    26101 aaatgcttat tcaatagacg aagcgagcaa agcaattaaa gaagcagaaa aagcaagaaa
    26161 agcaagaaaa aaagaagtgg acaagcaata tgaagatgat gtcattgcta taaaaaataa
    26221 cgtcaacctt tctaagtctg aaaaagataa attgttagct attgctgatc aaagacataa
    26281 agatgaagta agaaaagcaa aatctaaaaa agatgctgta gtagatgttg ttaaaaagca
    26341 aaataaagat attgataaag aaatggattt atccagtgga cgtgtatata aaaatactga
    26401 aaagtggtgg aatggcctta aaagttggtg gtctaacttt agagaagacc aaaagaaaaa
    26461 aagcgataaa tacgctaaag aacaagaaga aacagctcgt agaaacagag aaaatataaa
    26521 gaaatggttt ggaaatgctt gggacggcgt aaaaagtaaa actggcgaag cctttagtaa
    26581 aatgggcaga aatgctaatc attttggcgg cgaaatgaaa aaaatgtgga gcggaatcaa
    26641 agggattcca agcaaattaa gttcaggttg gagctcagcc aaaagttctg taggatacca
    26701 cactaaggct atagctaata gtactggtaa atggtttgga aaagcttggc aatctgttaa
    26761 atcgacaaca ggaagtattt acaatcaaac taagcaaaag tattcagatg cttcagataa
    26821 agcttgggcg cattcaaaat ctatttggag aggcacatca aaatggttta gcaatgcata
    26881 taaaagtgca aagggctggc taacggatat ggctaataaa tctcgcgcga aatgggataa
    26941 tatttctagt acagcttggt cgaatgcaaa atccgtttgg aaaggaacat cgaaatggtt
    27001 tagtaactca tacaaatctt taaaagattg gactggggat atgtattcaa gagcccacga
    27061 tcgttttgat gcaatttcaa gttcggcatg gtctaacgct aaatcagtat ttaatggttt
    27121 tagaaaatgg ctatccaaaa catatgattg gattagagat attggtaaag acatgggaag
    27181 agctgcggct gatttaggta aaaatgttgc taataaagct attggcggtt tgaatagcat
    27241 gattggcggt attaataaaa tatctaaagc cattactgat aaaaatctca tcaagccaat
    27301 acctacattg tctactggta ctttagcagg aaagggtgta gctaccgata attcgggagc
    27361 attaacgcaa ccgacatttg ctgtattaaa tgatagaggt tctggaaacg ccccaggtgg
    27421 tggagttcaa gaagtaattc acagggctga cggaacattc catgcacccc aaggacgaga
    27481 tgtggttgtt ccactaggag ttggggatag cgtaataaat gctaatgaca ctctgaagtt
    27541 acagcgtatg ggtgttttac caaagtttca tggaggtacg aaaaagaaag attggctaga
    27601 ccaacttaaa ggtaatatag gtaaaaaagc aggagaattt ggagctacag ctaaaaacac
    27661 agcgcataat atcaaaaaag gtgcagaaga aatggttgaa gcagcaggcg ataaaatcaa
    27721 agatggtgca tcttggttag gcgataaaat cggcgatgtg tgggattacg tacaacatcc
    27781 agggaaacta gtaaataaag taatgtcagg tttaaatatt aattttggag gcggagctaa
    27841 cgctacagta aaaatagcta aaggcgcata ctcattgctc aaaaagaaat tagtagacaa
    27901 agtaaaatcg tggtttgaag atttcggtgg tggaggcgat ggaagctatc tatttgaata
    27961 tccaatctgg caaagatttg gacgctacac aggtggactt aactttaatg gcggtcgtca
    28021 ctatggtata gactttggta tgccttctgg aacaaacgtt tatgccgtta aaggtggtat
    28081 agcagataag gtatggactg attacggtgg cggtaattct atacaaatta agactggtgc
    28141 taatgaatgg aactggtata tgcatttatc taagcaatta gcaagacaag gccaacgtat
    28201 taaagctggt caactgatag ggaaatcagg tgctacaggt aatttcgtta gaggagcaca
    28261 cttacatttc caattgatgc aagggtcaca tccagggaat gatacagcta aagatccaga
    28321 aaaatggttg aagtcactta aaggtagtgg cgttcgaagt ggttcaggtg ttaataaggc
    28381 tgcatctgct tgggcaggcg atatacgtcg tgcagcaaaa cgaatgggtg ttaatgttac
    28441 ttcgggtgat gtaggaaata ttattagctt gattcaacac gaatcaggag gaaatgcagg
    28501 tataactcaa tctagtgcgc ttagagacat caacgtttta cagggcaatc cagcaaaagg
    28561 attgcttcaa tatatcccac aaacatttag acattatgct gttagaggtc acaacaatat
    28621 atatagtggt tacgatcagt tattagcgtt ctttaacaac agatattggc gctcacagtt
    28681 taacccaaga ggtggttggt ctccaagtgg tccaagaaga tatgcgaatg gtggtttgat
    28741 tacaaagcat caacttgctg aagtgggtga aggagataaa caggagatgg ttatcccttt
    28801 aactagacgt aaacgagcaa ttcaattaac tgaacaggtt atgcgcatca tcggtatgga
    28861 tggcaagcca aataacatca ctgtaaataa tgatacttca acagttgaaa aattgttgaa
    28921 acaaattgtt atgttaagtg ataaaggaaa taaattaaca gatgcattga ttcaaactgt
    28981 ttcttctcag gataataact taggttctaa tgatgcaatt agaggtttag aaaaaatatt
    29041 gtcaaaacaa agtgggcata gagcaaatgc aaataattat atgggaggtt tgactaatta
    29101 atgcaatctt ttgtaaaaat catagatggt tacaaggaag aagtaataac agattttaat
    29161 cagcttatat ttttagatgc aagggctgaa agtccaaaca ccaatgataa cagtgtaact
    29221 attaacggag tagatggtat tttaccgggc gcaattagtt ttgcgccttt ttcattagta
    29281 ttaaggtttg gctatgatgg tatagatgtt atagatttaa atttatttga gcattggttt
    29341 agatctgtgt ttaatcgcag acatccttat tatgttatta cttctcaaat gcctggtgtt
    29401 aaatatgcag tgaatacagc taatgttaca tctaatttaa aagatggttc ttcaactgaa
    29461 attgaagtaa gtttaaatgt ttataaaggg tattctgaat cagttaattg gaccgatagc
    29521 gagttcttat tcgactctaa ttggatgttt gaaaatggaa ttcctcttga tttcacacct
    29581 aaatatactc atacatcaaa tcaatttact atttggaacg gttctactga tacgataaat
    29641 ccacgattca agcacgattt gaaaatatta attaatttaa atgcgagtgg aggatttgaa
    29701 ctggttaact atacaacagg tgatattttt aagtacaaca aaagtataga taaaaacact
    29761 gattttgttt tagatggtgt gtatgcatat cgagatataa atagagtggg aattgataca
    29821 aatagaggca ttataacatt agcgccaggt aaaaatgaat ttaagattaa aggagacgtc
    29881 agtgatatta aaactacatt taagtttcct tttatttata ggtaggtgat ttaatggatt
    29941 atcatgatca tttatcagta atggatttta atgaattgat ttgtgaaaat ttactagatg
    30001 tagattatgg ttcttttaaa gaatattatg aactgaatga agctaggtac atcaccttta
    30061 cagtttatag aactactcat aatagttttg tttttgattt attgatttgt gaaaacttca
    30121 taatttatca tggtgaaaaa tatacaatta agcagacagc gccaaaggtt gaaggtgata
    30181 aagtttttat tgaagttacg gcatatcaca taatgtatga atttcaaaat cactcagtgg
    30241 aatcaaataa gcttgatgac gacagtagcg aaactggtaa aacgccagaa tactctttag
    30301 atgagtactt aagatatgga tttgcaaatc aaaaaacttc ggtcaaaatg acctataaaa
    30361 taattggaga ttttaagcga aaagtaccga ttgacgaatt aggtaacaaa aacggcttag
    30421 aatactgtaa agaagcggta gacctgtttg gctgtataat ttacccaaat gatacagaga
    30481 ttggttttta ttctcctgaa acattttatc aaagaagcga gaaagtgatt cgatatcaat
    30541 ataatactga tactgtatct gcaactgtca gtacattgga attaagaaca gctataaaag
    30601 tttttggaaa aaagtataca gctgaggaaa agaaaaatta taatcctatt agaacaactg
    30661 acattaaata ttcaaatggt tttataaaag aaggtactta tcgtaccgaa acaattgggt
    30721 ctaaagctac tattaacttt gattgcaagt atggtaatga aacagttaga tttacaataa
    30781 aaaagggctc tcaaggtgga atatataagt tgattttaga cggcaagcaa attaagcaaa
    30841 tttcttgttt tgctaagtcg gttcagtctg aaacaataga tttaataaaa aatattgata
    30901 aaggcaagca cgttttagaa atgatatttt taggagaaga ccccaaaaat agaattgata
    30961 tatcttcaaa taaaaaagct aagccttgta tgtatgttgg aactgaaaaa tcaacagtct
    31021 taaatttaat tgctgataat tcaggtcgca atcaatacaa agcaattgtc gactacgtcg
    31081 cagatagtgc aaagcagttt gggattcgat atgctaatac gcaaacaaat gaagatatcg
    31141 aaacacagga taagctgtta gaatttgcaa aaaagcaaat aaatgatact cctaagactg
    31201 aattagatgt taattatata ggttatgaaa aaatagagcc aagagatagc gtattttttg
    31261 ttcatgaatt aatgggatat aacactgaat taaaggttgt taaacttgat aggtcacatc
    31321 catttgtaaa cgcaatagat gaagtgtctt tcagcaatga aataaaagat atggtacaaa
    31381 ttcaacaagc acttaacaga cgagttattg cacaagataa tagatataac tatcaagcaa
    31441 atcgtataaa tcatttatac actagtactt tgaattctcc tttcgagaca atggatatag
    31501 ggagtgtatt aatataatgg caacagaaga agttaaaatc aaagcgctac ttgaaaacga
    31561 taaacagtac tttccagcta cacactggaa agctataaat gggatacctt atgcaggcag
    31621 tagtgatatt gatggattgc ctcaagacgg tatcatttcg gtagatgata aaaataaatt
    31681 agataaatta aaaataggcg aagcaggaat tattcaaaat agcattgtac agaaatcccc
    31741 aaacggtaaa ttgtggaaaa taacagttga cgatagtggg aaacttggta cagtgctatt
    31801 ttattagaaa ggaaggtgca ttatggaaaa tttgtattta ataaaggatt tgggagcttt
    31861 agcaggtcga gattatagag ctaaagaaat tcaaaacctg caaagaatag agcaatttgc
    31921 gcttggcttg acaacagagt ttaagttgca tcagaaagct aaaacaatgc aacacttcgc
    31981 tgagcaaatt tattataatg gtagatcgca agcagcagta aacaaatctt tacaaagtca
    32041 aattaacgca cttgttgtgg caccacgtaa taacagtgct aatgagattg ttcaagctcg
    32101 agttaatgta aacggcgaaa cctttgacac attaaaagaa catttagacg attgggaaac
    32161 caaaactcaa attaataaag aggaaactat aagagaatta aataagacca aacaagaaat
    32221 tcttgatatc gagtatcgtt ttgaacctga taagcaagag tttttatttg tgacagaact
    32281 tgcacctctt acaaatgcag taatgcaatc cttctggttt gataatagaa caggcatagt
    32341 atacatgaca caagctagaa ataatggcta tatgctaagt cgtttaagac ctaatggtca
    32401 atttatagac agctcattga ttgtaggtgg gggtcatggt acacataacg gttatagata
    32461 tattgatgat gagttatgga tttatagttt tatcttaaat ggtaataatg agaatacatt
    32521 agttcgtttc aagtatacgc ctaatgtgga aattagctat ggcaagtatg gtatgcaaga
    32581 tgtatttaca ggacacccag aaaaacccta catcacccct gtcataaatg aaaaagaaaa
    32641 taaaattcta tacagaattg agagacctag aagtcagtgg gaacttgaaa actcaatgaa
    32701 ttatatagag ataagaagtt tagacgatgt tgataaaaat attgataaag ttttgcataa
    32761 aatcagtatc cctatgagac taacaaacga aacccaacca atgcagggtg tgacttttga
    32821 tgaaaaatac ttgtattggt atacaggaga cagtaatcca aataatagaa actatttaac
    32881 ggctttcgat ttagaaacag gagaagaagc gtatcaggtt aatgctgact atggtggaac
    32941 actagattca tttcctggcg aatttgcgga agcagaaggt ttgcaaatat actatgacaa
    33001 agatagtggt aaaaaagctt tgatgctagg tgttactgtc ggtggtgatg gaaatagaac
    33061 acatcgtatt ttcatgattg ggcaaagagg tattttagaa atacttcact caagaggcgt
    33121 tccttttatc atgagtgaca caggtggtag agttaaacct ttaccaatga ggcctgataa
    33181 acttaagaat cttgggatgt taacagagcc aggtctttac tatttataca ctgatcatac
    33241 agttcaaatc gatgatttcc cattaccaag agaatggcgt gatgcaggtt ggttcttgga
    33301 agttaagcca ccacaaactg gcggtgatgt aattcagata ttgacgcgta atagttatgc
    33361 aaggaatatg atgacttttg aaagggtgct ttctggaaga actggagaca tttcggactg
    33421 gaattatgtg cctaaaaata gtggtaaatg ggagagagta ccttcattca tcacaaaaat
    33481 gtcagatatt aacatagtag gcatgtcgtt ttatttaact acggatgata caaaacgttt
    33541 tacagatttt ccaactgaac gtaaaggggt agctggttgg aacttatatg tagaagcttc
    33601 aaacacaggt ggctttgttc ataggctagt tcgtaatagt gttacagcat ctgctgagat
    33661 actattgaaa aattatgata gtaaaacaag ttcagggcca tggactttac acgaagggag
    33721 aattataagt taatgagtaa tttagagaaa tctgtagcta taaatttaga aaacacagcg
    33781 cattatgaaa atatttcaaa tctagatata acttttagaa caggagagag tgattcttct
    33841 gttcttcttt ttaatatcat taaaaataat caaccgttat tactgagtga agaaaatatc
    33901 aaagcacgaa tagcgattcg aggtaaagga gtaatggtag ttgctccact agaaatatta
    33961 gatccattta aaggtatttt aaaatttcaa ttacctaatg atgtaattaa aagagatgga
    34021 agttatcaag ctcaagtttc ggttgcagaa ttaggtaatt cagacgtggt agttgtagag
    34081 agaactatca catttaacgt tgaaaaaagt ttgtttagca agattccctc tgaaacaaaa
    34141 ctacactata ttgttgagtt tcaagaatta gaaaaaacta ttatggatcg cgcgaaagca
    34201 atggacgagg ctataaaaaa tggtgaggat tatgcgagtc tgattgaaaa agctaaagaa
    34261 aaaggtctat cagatattca aatagcaaaa tcttcaagta ttgatgaatt aaagcaactt
    34321 gctaatagcc gtatatctga tttggaaaat aaagcgcaag catattcaag aacattcgat
    34381 gagcaaaagc gatatatgga tgagaaacat gaagccttca agcagtcagt gaatagtggt
    34441 ggtttagtca caagtggttc tacttcaaat tggcaaaaag ctaagattac taaagatgat
    34501 ggtaagataa tgcagattac tggatttgat tttaataatc cagaacaaag aataggtgat
    34561 tcaacccaat ttatttatgt ttcgcaagct ataaattatc caagagatgt tagtactaac
    34621 ggtactgtcg aatatttagt agtaacttca gattacaagc gtatgactta tcgaccgaac
    34681 ggtacaaata aagtgtttgt taaaagaaaa gaagcgggtt catggtctga gtggtcagaa
    34741 ttagctatta atgattacaa tacacctttt gaaactgttc aaagtgccca atcaaaagct
    34801 aatatggccg aaagtaacgc taaattatac gcagatgaca agtttaataa aaggtattcg
    34861 gttatttttg atggaacagc aaatggtgtg ggctctacat tgtacttaaa tgagagttta
    34921 gaccaattta ttttattaat tttttatggg acttttccag gtggtgactt tacagagttt
    34981 ggcagtcctt ttggaggagg aaagatttca ttgaatccct caaatcttcc agatggtgat
    35041 ggaaatggtg gaggtgttta tgagtttgga ttaactaaat ctagtcgtac atctttaact
    35101 atatcaaacg atgtctattt cgacttagga agtcaaagag gctctggtgc gaacgcaaat
    35161 agagggacaa ttaacaaaat tataggagtg agaaaataat gcaaatatta gttaacaagc
    35221 gtaatgagat aatttcatac gctatcattg gcggctttga agaaggtatt gatattgaaa
    35281 atttaccaga aaatttctct caagttttta gacctaaagc ctttaaatat tcaaatgggg
    35341 aaatagtttt taacgaagat tattcagaag aaaaagatga cttgcatcaa cagattgaca
    35401 gtgaagaaca aaacacagtc gcttctgatg acatcttacg aaaaatggtt gctagtatgc
    35461 agaaacaagt tgttcaaagt acaaagttat cgatgcaagt taataagcaa aatgcactaa
    35521 tggcaaaaca acttgtgaca cttaataaaa aattagaaga ggttaaagga gagactgaaa
    35581 atgcttaaat taatttcacc aacattcgaa gatattaaaa catggtatca attgaaagaa
    35641 tatagtaaag aagatatagc gtggtatgta gatatggaag ttatagataa agaggaatat
    35701 gcaattatta caggagaaaa gtatccagaa aatctagagt cataggttat aatcttatgg
    35761 ctttttaatt tgaataaagt gggtggtgta atgtttggat ttaccaaacg acacgaacaa
    35821 gattggcgtt taacgcgatt agaagaaaat gataagacta tgtttgaaaa attcgacaga
    35881 atagaagaca gtctgagaac gcaagaaaaa atttatgaca agttagatag aaatttcgaa
    35941 gaactaaggc gtgacaaaga agaagatgaa aaaaataaag agaaaaatgc taaaaatatt
    36001 agagacatca agatgtggat tctaggatta atagggacga ttctaagtac atttgttata
    36061 gccttgttaa aaactatttt tggcatttaa aggaggtgat caccatgctt aagggaattt
    36121 taggatatag cttttggtcg tgtttctggt ttggtaagtg taagtaatat ttaagagtca
    36181 gtgcttcggc actggctttt tattttggat aaaaggagca aacaaatgga tgcaaaagta
    36241 ataacaagat acatcgtatt gatcttagca ttagtaaatc aattcttagc gaataaaggt
    36301 ataagtccga taccagtaga tgaagaaagt gtttcatcga ttatcttaac agttgttgct
    36361 ttatatacta catataaaga taatccaaca tctcaagaag ggaaatgggc gaatcaaaaa
    36421 ttaaagaaat ataaagctga aagtaaatat agaaaagcaa cagggcaagc gccaattaaa
    36481 gaagtaatga cacctacgaa tatgaacgac acaaatgatt tagggtaggt gttgaccaat
    36541 gttgataaca aaaaaccaag cagaaaaatg gtttgataat tcattaggga agcagttcaa
    36601 tcctgatttg ttttatggat ttcagtgtta tgattacgcc aatatgttct ttatgttagc
    36661 gacaggcgaa aggctgcaag gtttatatgc ttataatatc ccgtttgata ataaagcaaa
    36721 gattgaaaaa tatggtcaaa taattaaaaa ctatgacagc tttttaccgc aaaagttgga
    36781 tattgtcgtt ttcccgtcaa agtatggtgg cggagctgga cacgttgaaa ttgttgagag
    36841 cgcaaattta aacactttca catcatttgg tcaaaactgg aacggtaaag gttggactaa
    36901 tggcgttgcg caacctggtt ggggtcctga aactgtgaca agacatgttc attattatga
    36961 caatccaatg tattttatta ggttaaactt ccctaacaac ttaagcgttg gcaataaagc
    37021 taaaggtatt attaagcaag cgactacaaa aaaagaggca gtaattaaac ctaaaaaaat
    37081 tatgcttgta gccggtcatg gttataacga tcctggagca gtaggaaacg gaacaaacga
    37141 acgcgatttt atacgtaaat atataacgcc taatatcgct aagtatttaa gacatgcagg
    37201 acatgaagtt gcattatacg gtggctcaag tcaatcacaa gatatgtatc aagatactgc
    37261 atacggtgtt aatgtaggca ataaaaaaga ttatggctta tattgggtta aatcacaggg
    37321 gtatgacatt gttctagaaa tacatttaga cgcagcagga gaaagcgcaa gtggtgggca
    37381 tgttattatc tcaagtcaat tcaatgcaga tactattgat aaaagtatac aagatgttat
    37441 taaaaataat ttaggacaaa taagaggtgt aacacctcgt aacgatttac taaatgttaa
    37501 tgtatcagca gaaataaata tcaattatcg cttatctgaa ttaggtttta ttactaataa
    37561 aaatgatatg gattggatta agaaaaatta cgacttgtac tctaaactaa tagctggtgc
    37621 gattcatggt aagccaattg gtggagtggt agctagtgag gtcaaagcgc cagttaaaaa
    37681 cgaaaagaat ccgccagtgc cagcaggtta tacactcgat aagaataatg tcccttataa
    37741 aaaagaacaa ggcaattaca cagtagctaa tgttaaaggt aataatgtaa gagacggtta
    37801 ttcaactaat tcaagaatta caggggtatt acccaacaac acaacaatta cgtatgacgg
    37861 tgcatattgt attaatggtt atagatggat tacttatatt gctaatagtg gacaacgtcg
    37921 ctatattgcg accggagagg tagacatagc aggcaaccga ataagcagtt ttggtaagtt
    37981 tagtgcagtt tgataattgt atatgatgaa tcttaggcag gtacttcggt acttgcctat
    38041 tatttaaaat taataaacag ttaattttta catgaatata ttaaatttta aaaaaacaaa
    38101 cgtttttagt atataaatta ttttgtgttc gtattgtgtg ctatgattaa aaagttgtta
    38161 tgg
//
LOCUS       DAKTLH010000011        37766 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.011, whole genome shotgun sequence.
ACCESSION   DAKTLH010000011 DAKTLH010000000
VERSION     DAKTLH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 37766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37766
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.011"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            345..809
                     /gene="accB"
                     /locus_tag="O0394_000476"
     CDS             345..809
                     /gene="accB"
                     /locus_tag="O0394_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCY0498580.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQAQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            809..2164
                     /gene="accC"
                     /locus_tag="O0394_000477"
     CDS             809..2164
                     /gene="accC"
                     /locus_tag="O0394_000477"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCY0498581.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            2179..2541
                     /locus_tag="O0394_000478"
     CDS             2179..2541
                     /locus_tag="O0394_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCY0498582.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            2601..2990
                     /gene="nusB"
                     /locus_tag="O0394_000479"
     CDS             2601..2990
                     /gene="nusB"
                     /locus_tag="O0394_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCY0498583.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKGHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            3007..4344
                     /locus_tag="O0394_000480"
     CDS             3007..4344
                     /locus_tag="O0394_000480"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCY0498584.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            4337..4567
                     /locus_tag="O0394_000481"
     CDS             4337..4567
                     /locus_tag="O0394_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCY0498585.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            4545..5426
                     /locus_tag="O0394_000482"
     CDS             4545..5426
                     /locus_tag="O0394_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCY0498586.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDTTKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
     gene            5858..6310
                     /gene="argR"
                     /locus_tag="O0394_000483"
     CDS             5858..6310
                     /gene="argR"
                     /locus_tag="O0394_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCY0498587.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            6326..8005
                     /gene="recN"
                     /locus_tag="O0394_000484"
     CDS             6326..8005
                     /gene="recN"
                     /locus_tag="O0394_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCY0498588.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELISDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR"
     gene            8156..9577
                     /gene="lpdA"
                     /locus_tag="O0394_000485"
     CDS             8156..9577
                     /gene="lpdA"
                     /locus_tag="O0394_000485"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCY0498589.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDTNEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            9593..10585
                     /locus_tag="O0394_000486"
     CDS             9593..10585
                     /locus_tag="O0394_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HCY0498590.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEERETLKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            10585..11568
                     /locus_tag="O0394_000487"
     CDS             10585..11568
                     /locus_tag="O0394_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCY0498591.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGL
                     QQKYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            11581..12855
                     /locus_tag="O0394_000488"
     CDS             11581..12855
                     /locus_tag="O0394_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCY0498592.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASATVEQTSTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTAEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIIKKPVVINDMIAIRNMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            13206..13643
                     /locus_tag="O0394_000489"
     CDS             13206..13643
                     /locus_tag="O0394_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCY0498593.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            13657..14637
                     /locus_tag="O0394_000490"
     CDS             13657..14637
                     /locus_tag="O0394_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCY0498594.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEVLLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(14764..14943)
                     /gene="prli42"
                     /locus_tag="O0394_000491"
     CDS             complement(14764..14943)
                     /gene="prli42"
                     /locus_tag="O0394_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HCY0498595.1"
                     /translation="MLVISSQVKSFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
     gene            15206..16339
                     /locus_tag="O0394_000492"
     CDS             15206..16339
                     /locus_tag="O0394_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCY0498596.1"
                     /translation="MINEQRLLNTFFELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAVVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARHSK"
     gene            16407..17813
                     /gene="gndA"
                     /locus_tag="O0394_000493"
     CDS             16407..17813
                     /gene="gndA"
                     /locus_tag="O0394_000493"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCY0498597.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            18032..18403
                     /locus_tag="O0394_000494"
     CDS             18032..18403
                     /locus_tag="O0394_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0498598.1"
                     /translation="MEKLNQVMLYVDDVEKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKHSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQDGQGNYFAVSD"
     gene            18342..18596
                     /locus_tag="O0394_000495"
     CDS             18342..18596
                     /locus_tag="O0394_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498599.1"
                     /translation="MVNVYLIFKMAKVTILQLVTKQSILIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLVLYSGWKRFPMQKLTGGD"
     gene            18597..19616
                     /locus_tag="O0394_000496"
     CDS             18597..19616
                     /locus_tag="O0394_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0498600.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETIASQNKINYQI
                     IETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTCIDIKPRMLGQQAGAAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            19632..21281
                     /locus_tag="O0394_000497"
     CDS             19632..21281
                     /locus_tag="O0394_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCY0498601.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDFG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
     gene            complement(21364..22230)
                     /locus_tag="O0394_000498"
     CDS             complement(21364..22230)
                     /locus_tag="O0394_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCY0498602.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(22460..23944)
                     /locus_tag="O0394_000499"
     CDS             complement(22460..23944)
                     /locus_tag="O0394_000499"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCY0498603.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
     gene            24257..25177
                     /gene="rnz"
                     /locus_tag="O0394_000500"
     CDS             24257..25177
                     /gene="rnz"
                     /locus_tag="O0394_000500"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCY0498604.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKI"
     gene            complement(25282..26097)
                     /gene="proC"
                     /locus_tag="O0394_000501"
     CDS             complement(25282..26097)
                     /gene="proC"
                     /locus_tag="O0394_000501"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCY0498605.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ"
     gene            26239..26994
                     /locus_tag="O0394_000502"
     CDS             26239..26994
                     /locus_tag="O0394_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0498606.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELGKTINVIPCDLNDLKSIQALQFEDNTSFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGEIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            27010..27258
                     /locus_tag="O0394_000503"
     CDS             27010..27258
                     /locus_tag="O0394_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498607.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILGDISVDQISDGTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(27474..28382)
                     /locus_tag="O0394_000504"
     CDS             complement(27474..28382)
                     /locus_tag="O0394_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0498608.1"
                     /translation="MQKNILKSGISLSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIFIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            28464..29006
                     /locus_tag="O0394_000505"
     CDS             28464..29006
                     /locus_tag="O0394_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCY0498609.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            29111..29560
                     /locus_tag="O0394_000506"
     CDS             29111..29560
                     /locus_tag="O0394_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCY0498610.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            29609..30496
                     /gene="xerD"
                     /locus_tag="O0394_000507"
     CDS             29609..30496
                     /gene="xerD"
                     /locus_tag="O0394_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCY0498611.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(30574..31080)
                     /locus_tag="O0394_000508"
     CDS             complement(30574..31080)
                     /locus_tag="O0394_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HCY0498612.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPVFERIIKANYPDYDYNQETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            31172..31903
                     /locus_tag="O0394_000509"
     CDS             31172..31903
                     /locus_tag="O0394_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCY0498613.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            31896..32438
                     /gene="scpB"
                     /locus_tag="O0394_000510"
     CDS             31896..32438
                     /gene="scpB"
                     /locus_tag="O0394_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCY0498614.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKDQLVELIENYSS
                     HGLMIQRFGMTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            32431..33168
                     /locus_tag="O0394_000511"
     CDS             32431..33168
                     /locus_tag="O0394_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCY0498615.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            33301..34026
                     /gene="srrA"
                     /locus_tag="O0394_000512"
     CDS             33301..34026
                     /gene="srrA"
                     /locus_tag="O0394_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HCY0498616.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            34007..35758
                     /gene="srrB"
                     /locus_tag="O0394_000513"
     CDS             34007..35758
                     /gene="srrB"
                     /locus_tag="O0394_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HCY0498617.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
     gene            36003..36932
                     /locus_tag="O0394_000514"
     CDS             36003..36932
                     /locus_tag="O0394_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCY0498618.1"
                     /translation="MKKFIGSVLATTLILGGCSTMENESKKDTKTETKSVPEEMEASK
                     YVGQGFQPPAEKNAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVH
                     CEDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKYDNLY
                     KFFKENEKKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIRKNDK
                     EFKEGMERARKEVNYAANTDAVATLFSTKKNFTKDNTVDDVIELSDKLYNLKNKPDKS
                     TITIQIGKPTINTKKAFYDDNRPIEYGVHSKDE"
     gene            36925..>37766
                     /locus_tag="O0394_000515"
     CDS             36925..>37766
                     /locus_tag="O0394_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498619.1"
                     /translation="MNKINDRDLTELSSYWVYQNIDIKKEFKVNGKRFKQVDSYNDDK
                     NSNLNGAADIKIYELLDDKSKPTGQQTIIYQGTSNEAINPNNPLKSSGFGDDWLQNAK
                     LMNNDNESTDYLKQTDQLSNQYKIKLEDADRLSNSDFLKKYRMESSNFKNKTIVADGG
                     NSEGGAGAKYQGAKHPNEKVVATDSAMIPYAAWQKFARPRFDNMISFNSTNDLLTWLQ
                     DPFIKDMPGKRVNINDGVPRLDTLIDSHVGYKRKLNRKDNTYDTVPLIKIKSVKDTEI
                     KNGK"
BASE COUNT        13778 a         5286 c         7002 g        11700 t
ORIGIN      
        1 aaaataattt ttaaaatata aaacgaatcg tctaattggt agtgaaaaaa catagacatg
       61 agtagaggta tatatctaca tatcatattg ttgaggataa aacataatag ctaataaaaa
      121 taacattgat atgtttaact gaatgcataa aatttttaat caagagttaa gatgagtcaa
      181 tgttaagtaa taaatacaca agtgttttta atttaaaatg tggttttatt ataactttgg
      241 gatagactta taatgtcgcc agcaatatga acatgcttga attgaagagt tgtctcaagt
      301 aaaatagacg ggtagatgaa aacaaactga aggagtcagt aataatgaac tttaaagaaa
      361 tcaaagaatt aattgaaatt ctggataaat caactttaac ggaaatcaat attgaagata
      421 ctaaaggcaa agtgacgctt aagaaagaaa aagaaactga gattatcacg ccacaaatct
      481 cacaaatgcc agttgaagct gcggcaatgc ctatgcctca agcacaatca actgatagca
      541 ataaaactga agctccaaag ccaacttcag ataatcacaa aacaattaat gcacctatgg
      601 taggtacatt ttacaaatcg ccatctccag acgaagaagc atatgtgcaa gttggggaca
      661 ctgtttcaaa tgaaacaaca gtgtgtattt tagaggcaat gaaactattt aatgaaattc
      721 aagcagaaat ttcaggtgaa attgttgaaa tcttagtaga agacggacaa atggtagagt
      781 atggccaacc gttatttaag gtgaaataat gaaaaaggtt ttaattgcaa accgcggtga
      841 aatcgcagtt aggattattc gcgcttgtcg tgatttaggc atccaaactg ttgcaatcta
      901 ttctgaaggg gataaagatg cgctacatac tcaaattgct gatgaagcat attgcgtagg
      961 tcccactttg tctaaagatt catatttaaa tattccgaac atcttatcta ttgcaacttc
     1021 tacaggttgt gatggcgttc atccgggtta tggcttttta gctgaaaatg ctgattttgc
     1081 agaattatgc gaagcatgcc aattgaagtt cattggacca agttatcaat ctatccaaaa
     1141 aatgggtatc aaagatgttg ctaaggcaga aatgatcaaa gccaatgttc cagttgttcc
     1201 tggtagtgac ggtttaatga aagacgtctc agaagctaag aaaatcgcca aaaaaattgg
     1261 ctatccggtc atcattaaag ctactgctgg cggtggcgga aaaggtatcc gtgttgctcg
     1321 tgatgaaaaa gaacttgaaa ctggcttccg aatgacagaa caagaagctc aaactgcatt
     1381 tggtaatggt ggactttata tggagaaatt catcgaaaac ttccgccata ttgaaatcca
     1441 aattgttggg gacagctatg gtaatgtaat tcatttagga gaacgtgatt gtacaattca
     1501 aagacgtatg cagaaattag tggaagaagc accttcccca attttagatg atgaaacacg
     1561 tcgtgaaatg ggaaatgccg cagttcgtgc agcgaaagct gtaaattatg aaaatgcggg
     1621 aacaattgag tttatatatg atttaaatga taataaattt tattttatgg aaatgaatac
     1681 acgtattcaa gtagaacatc ctgtaactga aatggtaaca ggaattgatt tagttaaatt
     1741 acaattacaa gttgctatgg gtgacgtgtt accgtataaa caagaagata ttaaattaac
     1801 aggacacgca attgaattta gaattaatgc tgaaaatcct tacaagaact ttatgccatc
     1861 accaggtaaa attgagcaat atcttgcacc aggtggatat ggtgttcgaa tagagtcagc
     1921 atgttatact aattatacga taccgccata ttatgattcg atggtagcga aattaatcat
     1981 acatgaaccg acacgagatg aagcgattat ggctggcatt cgtgcactaa gtgaatttgt
     2041 ggttcttggt attgatacaa ctattccatt ccatattaaa ttattgaata acgatatatt
     2101 tagaagcggt aaatttaata caaacttttt agagcaaaat agcattatga atgatgaagg
     2161 ttaataggag gtcaatccat ggtcaaagta actgattatt caaattcaaa attaggtaaa
     2221 gtagaaatag cgccagaagt gctatctgtt attgcaagta tagctacttc ggaagtcgaa
     2281 ggcatcactg gccattttgc tgaattaaaa gaaacaaatt tagaaaaagt tagtcgtaaa
     2341 aatttaagcc gtgatttaaa aatcgagagt aaagaagatg gcatatatat agatgtatat
     2401 tgtgcattaa aacatggtgt taatatttca aaaactgcaa acaaaattca aacgtcaatt
     2461 tttaattcaa tttctaatat gacagcgata gaacctaagc aaattaatat tcacattaca
     2521 caaatcgtta ttgaaaagta atgtcatacc taattcagta attaaataaa gaaaaataca
     2581 aacgtttgaa ggagttaaaa atgagtcgta aagaatcccg agtgcaagct tttcaaactt
     2641 tatttcaatt agaaatgaag gacagtgatt taacgataaa tgaagcgata agctttatta
     2701 aagacgataa tccagattta gacttcgaat ttattcattg gctagtttct ggcgttaaag
     2761 gtcacgaacc tgtattagac gagacaatta gtccttattt aaaagattgg actattgcac
     2821 gtttattaaa aacggatcgt attattttaa gaatggcaac atatgaaata ttacacagtg
     2881 atacacctgc taaagtcgta atgaatgaag cagttgaatt aacaaaacaa ttcagtgatg
     2941 atgatcatta taaatttata aatggtgtat tgagtaatat aaaaaaataa aattgagtga
     3001 tgttatatgt cagattattt aagtgtttca gctttaacga aatatattaa atataaattt
     3061 gatcaagatc cacatctcca atctgtattg attaaaggtg agctttcaaa tttcaaaaag
     3121 cattcaagtg gtcatttata ctttaatgtg aaagataaag aaagcgttat aagtgccatg
     3181 atgttcaaag gtagtgcttc taaattaaac ttcgaaccta aagaaggaga tgaagtctta
     3241 ttagaagcac gtgtttctgt ttttgaacgt cgtggaaact atcaaattta tgtaaataaa
     3301 atgcaattag atggtatagg gaacttatat caaaaattag aagcattaaa gaaaaaatta
     3361 actgaagaag gttgttttga taaagcgaat aagaaatcaa tacctaagtt tcctaaaaaa
     3421 attgcagttt taacagcgag tacaggtgcc gcaattcgag atatccattc aacgataaat
     3481 agtcgttttc cattagctga acaaatacaa attagtactt tggttcaagg tgaaaaagca
     3541 aaagacgaca ttattgaaaa aattgaatat gcagatagtt taggtgtaga taccattatt
     3601 gtaggtcgag gcggtggatc catagaagat ttatggaatt tcaacgaaga agctgtcgtc
     3661 cgtgcaatat ataattgtaa gacaccgatt atatcagcag ttggtcatga aacagacttt
     3721 acattaagtg attttgctgc agacatcaga gctgcgactc caactcaagc tgctgttatt
     3781 gcaacaccag accaatatga attgctgcaa caaatacagc aatatcagtt tacattgact
     3841 cgtttcatca agaaacattt agaacaacaa cgtaaacatg ttgaacattt gtcatcgtat
     3901 tacaagttta aacaaccaac tttattgtat gatcaacaga tacaacgtcg agatgattta
     3961 gaaaagagac tgaaacaaca aattcaggca acttttgaac aacagagaca tcgattaatg
     4021 ctgttgcaac aacgttataa tttaaaagca ttactaagta gtgttaatca agaacaacaa
     4081 aataatcttc aattgacaaa ccaacttgtt aaattattga attctaagat attaagttat
     4141 aaaaatgatt tgaaaaataa agtagaaaac ttaaacaatt taagtccaac taatacaatg
     4201 ttgcgtggat atgcaattgt taataaaaaa gacgaagtca ttacgagtac taaagattta
     4261 acagaaaatg atcaattgac attaacaatg aaagatggct tagtagatgc aaaagttacg
     4321 aaagtaaggt gtaataatga ctaaagaaac gcaaagtttt gaagaaatga tgcaagaatt
     4381 agagcaaatt gttcaaaaat tagataatga aacagtatct ttagaggaat cattagattt
     4441 atatcaacgt ggtatgaaac tatcagcagc ttgtgacaca actttaaaaa atgccgaaaa
     4501 aaaggtgaat gacttaataa aagaagaagc tgaggatgta aaaaatgacg aatctaccga
     4561 tgaataaatt aatagatgaa gtcaataatg aattatcggt tgcgataaat aaatcagtaa
     4621 tggatactca gctagaagaa agtatgttgt attcattaaa tgctggaggt aaacgcatcc
     4681 gaccagttct gttattactc actttagatt cactaaatac cgagtatgag ttaggtatga
     4741 agagcgcaat tgcactagaa atgattcata catattcact tattcatgat gacctaccag
     4801 cgatggataa tgatgattat cgacgaggaa aattaacaaa tcataaagta tatggtgagt
     4861 ggactgcgat attagcaggt gatgctttat taactaaagc atttgaactt atttcaagtg
     4921 atgatagatt aactgatgaa gtaaaaataa aagttctaca acggctgtca atagcaagtg
     4981 gtcatgttgg aatggtcggc ggtcaaatgt tagatatgca aagcgaaggc caaccaattg
     5041 atcttgaaac tttggaaatg atacacaaaa caaaaacagg agcattatta acttttgcgg
     5101 ttatgagtgc agcagatatc gctaatgtcg atgatacaac taaagaacat ttagaaagtt
     5161 atagttatca tttaggtatg atgttccaga ttaaagatga tttattagac tgctatggtg
     5221 atgaagcaaa gttaggtaaa aaagtgggca gcgatcttga aaataataaa agtacgtacg
     5281 tgagtttatt agggaaagat ggcgcagaag ataaattgac ttatcataga gacgcagcag
     5341 tggatgaact aacgcaaatt gatgaacaat tcaatacaaa acacttatta gaaatcgttg
     5401 atttatttta tagcagggat cactaaaagc aaatatatcg attagcaaca attgtaaagt
     5461 ttgaaatgtc tcaaaaggca tattcaactg acgttgtttg ttaatcgatt tttctcatgt
     5521 tgaaacttaa aattatgttg aagactttca tttaaaaaat agaattttca atttctatac
     5581 gacaatattc gtatctgtga tatcaatgtg attttaatta aatatgttca aacaattgac
     5641 gcttattaaa tctaataatt agcaagttta ttacacatat attcattttt atacatattt
     5701 taagcaattg ttatgcgtaa tatgaaaaat atttataaat agataaccaa gatagataaa
     5761 ccatgtttac cctttttacg taaaggtgtt tagtagtcgt aaatcagtgc atgtgttaaa
     5821 ataaatgtat aaatattcgt aatcagaggt gtttactgtg cccaaaaaat cggttaggca
     5881 tataaaaatt agagaaatta tttcaaatga acagatagag acacaagatg aattagttaa
     5941 acgattaaac gattatgatt taaatgtcac tcaagcaact gtttctcgtg atattaaaga
     6001 actacaactt attaaagtac ctataccttc aggtcaatat gtttatagtt taccaaatga
     6061 tagaaaattc catcctttag aaaaattggg acgttattta atggattcct ttgttaatat
     6121 agatggtact gataatttac ttgttctaaa aacattacct ggtaatgcac aatctattgg
     6181 agctatatta gaccaaatca attgggaaga agtactaggc acaatttgtg gtgatgatac
     6241 ttgtttaatt atttgtcgaa gcaaagaggc aagtgatgaa atcaagtcaa gaattttcaa
     6301 tttgttataa ggatgcgatg agtttatgtt acaaacctta tcaatcaagc aatttgctat
     6361 tattgaagaa ttagaaattc aattttctga tggtttaact gttttaagtg gtgaaacggg
     6421 ttcaggtaaa tcaattatta tagatgcaat tggacaatta attggaatga gagcttcatc
     6481 tgattttgta agacatggtg aaaaaaaggc tgtcatcgaa ggtatatttg atattgacga
     6541 gagtaaagat gctatacata tattgaaaaa tatggatatt gatgtagatg aagatttttt
     6601 attagttaaa cgggaaattt tcagctctgg taaaagtctt tgtaaaatta ataatcaaac
     6661 agttacttta caagatttac gaaaagttat gcaagaatta ttagatattc atggccaaca
     6721 tgaaacgcag tctttgttga aacaaaaata ccatttaaca ttattggata attacgcaga
     6781 atcacgttat caagatttgt tggataaata tcaccaaact tttcaaaatt ataaagccaa
     6841 aaagcaagag ttagaagatt tagaatcagc agaccaagca ttgctacagc gtttagattt
     6901 aatgaaattc caattagaag aattgtctga agcacatcta aaagaaggcg aaatagagca
     6961 acttgaaata gatattaaac gcattcaaaa ctctgaaaaa ttaagcttag cgcttaataa
     7021 cgcacatatg acattaacgg atgaaaatgc tatcactgat cgtttatatg aactaagcaa
     7081 ccatttgtta acaataaacg atatagttcc aaacaaatat gacaaattaa aagaagatat
     7141 tgatcaattt tactacattt tagaagatgc aaaacatgag ttatatgatg aaatggctaa
     7201 tacagaattc gacgaacaag tgttaaacga gtatgaatct cgtatgaatt tgctgaataa
     7261 tttaaaacgt aaatatggaa aagatatttc agaattaatc gcatatcaag aaaagcttaa
     7321 taatgaaatc aataaaattg aaaactatga acaaagtaca tcgcagttac gagaagaaat
     7381 aaatgcattg tataatcaag ttatagaggt tggacaagcg ttatcaaagc agcgtcgtat
     7441 agtcgctagg gagttaagag accacattgt atcggaaatt caaaacttac aaatgaaaga
     7501 cgcaaatctt gagatttcat ttaaaaaatt agaagaaccg aatattgatg gaatcgaatt
     7561 tgtagaattt ttaatcagtc caaataaagg ggaaccatta aaaagtttaa ataaaattgc
     7621 gtcaggtgga gaactttcta gaattatgtt agcgttaaaa agtatttttg ttaaatcgag
     7681 aggtcaaact gcaattttat tcgatgaggt tgactcaggt gtatctggac aagctgcaca
     7741 aaaaatggca gagaaaatgc gagatatagc agaatatata caagttattt gtatctctca
     7801 cttaccacaa gttgcatcga tgagcgacca tcatttatta atttcgaaat catcaaaaga
     7861 tgatcgtaca acaacacaag ttcaagagtt aattagcgat gataaagtag atgaaattgc
     7921 acgaatgatt tcaggtgcaa gtgttactga tttaactcga gaaaatgcaa gagaaatgat
     7981 acaacacaat caaagacgta gataatttcc aaagtgtgtt gaatactcat ttatggattt
     8041 atttatgtgc attcaaacta aatagacaat catgtaaaat aggtaattaa tggacatgaa
     8101 aactatgtat taataaattt tcaatggata tatatcatta gttaggagta agaacatgtc
     8161 agagaaacaa tatgatttag tcgttctcgg tggaggtact gcaggatatg ttgctgcgat
     8221 acgtgcgtct caattaggta aaaaagtggc aatagtggaa cgacaactat taggaggtac
     8281 ttgtctccat aaaggttgta ttcctactaa atcgctgctt aaatctgctg aagtatttca
     8341 aacagtaaaa caggcagcaa tgtttggcgt tgatgtcaaa gatgctaatg ttaattttga
     8401 aaatatgttg gcacgaaaag aagacattat taatcaaatg tatcaaggtg taaagcattt
     8461 aatgcaacac aatcacattg acatttataa tggcacggga cgtattctag gtacatctat
     8521 attttcacct caaagtggca caatttctgt cgaatatgaa gatggtgaat cagacttatt
     8581 acccaaccaa tttgtattaa ttgcaactgg ttcttcgcct gcagagttgc cattcttatc
     8641 atttgatcat gataaaattt tatctagcga tgatatatta tcattaaaga cgttaccatc
     8701 aagtattggt attattggtg gtggtgttat cggaatggaa tttgcatcat tgatgataga
     8761 tttaggtgtt gacgtaacag ttatagaagc aggtgaaaga attttaccaa ctgaaagtaa
     8821 acaagcttca caactattaa aaaaatcatt gtcagcacgt ggtgttaaat tctatgaggg
     8881 aataaaactt tctgaaaatg atataaatgt taacgaagat ggtgttacgt ttgaaatttc
     8941 ctcagacata attaaagtag ataaagtgtt gctatcgatt ggtagaaaac ctaacacatc
     9001 agatattggt ttaaacaaca ctaaaataaa actttcaaca tcaggacata ttttaacgaa
     9061 cgaatttcaa caaactgaag ataaacatat ttatgcagct ggtgattgta taggaaaatt
     9121 acaattggca catgttggat caaaagaagg tgttgtggct gttgatcata tgtttgaggg
     9181 gaacccaatc ccagtaaact ataacatgat gcctaagtgt atttattcac aacctgaaat
     9241 tgcttctatc ggtttaaaca ttgaacaagc aaaggcagag ggaatgaaag ttaaaagttt
     9301 taaagtacca tttaaagcaa ttggtaaagc agtgattgat agccatgaca caaacgaagg
     9361 gtatagcgaa atggtgattg atcaatcaac tgaagaaatt gtgggtatta atatgattgg
     9421 tccacatgta acagaattga ttaatgaggc atcactgtta cagttcatga atggctcggc
     9481 attagaatta ggactaacaa cacacgcaca tccttccatc tctgaagtgt tgatggaatt
     9541 aggattgaaa gcagaaagta gagctattca cgtataaata ggaggattta acatgattga
     9601 ttataaatca ttaggcctta gcgaagaaga cctaaaagta atatataaat ggatggattt
     9661 aggtagaaaa atagatgaaa gactgtggtt attgaaccgt gcaggtaaaa ttccatttgt
     9721 tgtaagtggt caaggacaag aagcaactca gattgggatg gcatacgcat tagaagaagg
     9781 tgatattaca gcgccttatt atagagatct agctttcgtt acttatatgg gtatctcagc
     9841 gtatgatacg tttttatcag cgtttggaaa gaaagacgat gttaactctg gtggaaaaca
     9901 aatgccatca cactttagta gtagagctaa gaatattttg tctcaaagtt caccggtagc
     9961 aactcaaata ccccatgctg ttggtgctgc tttagcttta aaaatggatg gtaagaagaa
    10021 aattgcaaca gcaactgttg gtgaaggtag ctcaaaccaa ggtgacttcc atgaaggttt
    10081 aaacttcgcg ggtgtacaca aacttccttt tgtatgtgtc attataaata ataaatatgc
    10141 aatttcagta cctgattcat tacaatacgc agctgaaaaa ttatcggata gagcacttgg
    10201 ttacggtata catggtgaac aagtagatgg taatgatcca ttagcaatgt ataaagcgat
    10261 gaaagaagca agagatcgag ctatttctgg tcaaggttca actttgattg aagcagtaac
    10321 aagtcgtatg acagctcatt catcagatga tgacgaccaa tatcgtacga aagaagagcg
    10381 tgaaacgctt aaaaaagcag actgcaatga aaagttcaaa aaagagttgc tttcagctgg
    10441 cattatcgat gatgcttggt tggcagaaat agaagcggag cataaagaca tcatcaataa
    10501 agcgacaaag gctgctgaag acgcgccata tccaagtgtc gaagaagcct atgcatttgt
    10561 gtacgaagaa gggagtctta actaatggct aaattatctt atttagaggc gatacgccag
    10621 gcacaagact tagcattaca acaaaataaa gatgtgttta ttcttggtga agatgttggt
    10681 agaaaaggtg gcgtatttgg tactactcaa ggactgcaac aaaaatatgg tgaagacaga
    10741 gttatcgata cgccattagc tgagtcaaat attgttggaa ctgccattgg tgcagctatg
    10801 gtaggtaaaa gaccaattgc agaaattcaa tttgcagatt ttattttacc tgcgacaaat
    10861 caaattatta gtgaagctgc gaaaatgcgt tatcgttcaa ataatgattg gcaatgccca
    10921 ttaacgattc gtgcaccatt tggtggcggt gttcacggag gactatatca ttcacaaagt
    10981 attgaaagta tatttgcttc atcgccaggt ttaacaattg ttattccttc cacaccgtat
    11041 gatgctaaag ggcttttatt atcatctatc gaatcaaatg atccagtatt gtactttgag
    11101 cataaaaaag cttatcgttt cttaaaggaa gaagtgccag aagaatatta cactgtacct
    11161 ttaggaaaag cagatgtgaa acgtgaaggt gaagatttaa cagtattttg ttatggatta
    11221 atggtcaatt attgtttaca agcagcggat attttagctg cagacggtat caatgttgaa
    11281 gtcgttgatt tacgaactgt ttatccatta gataaagaaa caattattga tcgtgctaaa
    11341 catacaggta aagttttact tgtaactgaa gataatttag aaggtagtat tatgtcagaa
    11401 gtgtcagcga ttattgcaga gcattgcttg ttcgatttag atgcaccaat catgcgttta
    11461 gctgctccag atgtaccatc tatgccattt tctcctgtat tagaaaatga aattatgatg
    11521 aatccagaaa aaatcttaaa taaaatgcgt gaattagcag aattctaggg agggaaagtc
    11581 atggaaataa caatgcctaa gttaggtgag agtgttcatg aaggcaccat tgaacaatgg
    11641 ttagtttctg ttggtgatca tattgatgaa tatgaaccat tatgtgaagt tattacagat
    11701 aaagtgacag ctgaagtccc ttccacgata tcaggaacaa ttacagaaat tttagttgaa
    11761 gcggggcaga cagtagctat tgatacaatt atctgtaaaa ttgaaactgc tgatgaaaag
    11821 acaaatgaaa caactgaaga gatacaagca aaagtggatg agcatactca gaaatctact
    11881 aaaaaagcta gtgcaacagt ggaacagaca tctactgcta aacaaaatca accacgtaat
    11941 aatggtcgct tttcacctgt tgtatttaaa ctcgcttcag agcatgacat tgatttatca
    12001 caagttgtag gtagtggatt tgaaggtcgt gtaactaaga aggatataat gtcagttatt
    12061 gaaaatggtg gtaccacagc tcaatctgac aaacaagttc aaacaaaatc aacatcagta
    12121 gatacatcaa gtaaccaatc atctgaagac aatagtgaaa acagcacaat accagtaaat
    12181 ggtgtgcgta aagcaattgc gcaaaatatg gttaatagtg taacagagat tccacatgca
    12241 tggatgatga ttgaagtaga tgctacaaat cttgtgaata cgagaaatca ttataaaaac
    12301 agctttaaaa ataaagaagg atataatcta acgttctttg ctttctttgt aaaagctgta
    12361 gcagatgctt taaaagcata tcctttatta aatagtagct ggcaaggaaa tgaaattgtc
    12421 ttacataaag acattaatat ttcaattgct gttgctgatg aaaataaatt atacgtacct
    12481 gtgattaagc atgcagacga aaagtcaatc aaaggtatag ctagagaaat taatacttta
    12541 gcaacgaaag cgcgtaataa gcaattgaca gctgaagata tgcagggcgg tacatttacg
    12601 gtaaataata ctggtacatt tggttcagta tcatcaatgg gtattataaa tcatccacaa
    12661 gcagcgattt tacaagtaga atcaatcatt aaaaagccag tagtaattaa tgatatgatt
    12721 gcaattcgta acatggttaa tttatgtatt tcaattgatc atcgtatttt agatggttta
    12781 caaacaggta aatttatgaa tcatattaaa cagcgtatcg aacagtatac tttagaaaat
    12841 acaaatatat attagtgata acatagatgc atctatcgac aacttgtttt atcttgttct
    12901 tgtcgatgga tgtattattt tttggcacta aaatatgtgc aatatattca aaaaagataa
    12961 agaacaataa tcaacatggt tgaatgcatt tttgcagtaa agtcaaaata agacatcata
    13021 cttgaaacat attaatgaaa acatgtgaac aaattagtta ccatgatttt aagcacaata
    13081 atgtttggta tattgttaaa attgtgtcta aatataggtg tgattcagat tagtttattg
    13141 aacaatatgt tattaattag tagaatgagg atagtttaaa tataaaggga taggtgattg
    13201 aacttatgga catgaatttc gatttataca tgaacggtgt tgtagaacaa gcaaggaatg
    13261 aaattgaatc tgcgggatat gagcaattaa ctactgcaga agatgttgac aaagttctta
    13321 aacaagatgg tacaacacta gttatgatca attctgtatg tggttgtgca ggtggtatcg
    13381 caagaccagc agcatcacat gctttacatt atgacgtatt acctgatcgt ctagtgacag
    13441 tatttgctgg acaagataaa gaagcgacac aaagagcgcg tgaatacttc gaaggttatg
    13501 cgccttcaag tccgtcattt gcattagtaa aagatggaaa gattacagaa atgattgaaa
    13561 gacatcaaat cgaaggtcat gatgtgatga acgtaattaa tcaattacaa acattattca
    13621 ataaatattg tgaagaaaga taagaggcgc taacccatgt taaagttaaa tccttacaag
    13681 attggattta gaacaataaa aacagcagtg ggtatgactt taggtgtaat tattagtaag
    13741 ctgttaggtt tagataatta tgcttcaagc gccatattag tcgtattatg tattaaacat
    13801 acaaaagtac attcgctaca agcgattatt tcaagattag tatcatgttt tttagtattg
    13861 tttttaggtt cagcaatatt tagtttatta ggtcagagtc caattgtact cggtattatc
    13921 gtattgttat ttataccatt aactgtcgta ttaaaagtac aagaaggtgt cattacgagt
    13981 tgcgttatat tacttcatgt ttttaatgca aaatcaattg atgcacattt aattgttaat
    14041 gaaacattat tactgttaat tggactaagc attgcattta caatgaattt aatgatgcca
    14101 agtttagaca aacaactaga cgaatacaaa tgtaaaattg agcaacaaat tgctgatatt
    14161 tttagtaaat atagttatat ttgtgaaaaa tatgaagata ccattgcgat tgaatttgaa
    14221 gtgttacttt taaatattaa aaaggcgaag tctatcgcgt tccgagatgt taaaaatcat
    14281 tttgttagaa acgaaaattc atactatcat tattttgata tgcgagaaga gcaagtggaa
    14341 ttgttaatga gaatgaaacc gctcatcgaa agtatctgtc ataaagatcc gcttttaaat
    14401 gatgttgcgt cactgctgaa agaaattggt ctaaatgtga atagtaatga ctatacagca
    14461 atgcgtttac acaatttgta tgagacgcgt ttgaaattgg atgaactgcc attgccaaca
    14521 acacacaaag cgcttaactc gagagcaagt attattcaaa tattaaatga attagaagaa
    14581 tatttacaaa tcaaatcgca atttggttca ttgaaaatgc atagtgaagt agcttaaaaa
    14641 ataatccatc cgtatgagcc atgtttgaaa gtaatcgttg caatacgata acttattaat
    14701 acatgcgttt ataatggatg gatttttaat tgtatgattt tgtataggta gttgagatat
    14761 agtttataaa attggaatga tactcgttaa taacaatgct aatacaactg atacaagcat
    14821 aacgataatg ataattctta aaactttatt ttgcattttg tgtgcctcca aatgaataac
    14881 ctttgtatta ataattacga cataagatat aaaactttta acttgagacg atataactag
    14941 caatcatcat aaaggaatat aataaaaaac atgagtaagt ttgcttatgt cattttcctc
    15001 attatacgat atctttgaaa aatcaatcaa tatatttttg acaaagctta gtttgagtta
    15061 caataattat atataataaa ttgaaaataa tatgacacaa tttacaaacc tgataaatct
    15121 gtgtaacgtc aattactgta acattttatt tcatcaattt ttaccgtttg gcgtacatga
    15181 aaattttgat aaaaggggtt ccattatgat taatgagcaa agattactta atacgttttt
    15241 tgaacttgta caaatcgatt ctgagacagg gaatgaatca acaatccaac ctattttaaa
    15301 agaaaaattc atagcattag ggttagatgt taaagaagat gaagcggcta aacatcctaa
    15361 attaggtgcc aataatctag tgtgtacaat gaatagtact atcgaagaag gcgaagtacc
    15421 taaattatat ttgacgagcc atatggatac tgttgtaccc gcaattaatg taaagccaat
    15481 tgtaaaagat gacggctaca tatactctga tggtacgact attctaggtg cagatgataa
    15541 agcaggatta gcagcgatgc ttgaagtttt gcaagtgata aaggaacaac aaatcccaca
    15601 tggacaaatt caatttgtga ttactgttgg agaagaatca ggtttaattg gtgctaaaga
    15661 attaaattca gagttgttgg acgcggactt tggttatgct attgatgcaa gtgctgatgt
    15721 cggtactacg gttgtaggtg caccgacgca aatgttaatt tcagctaaaa tttttggcaa
    15781 aacggctcat gcaagtacgc caaaagaggg tgttagtgct attaatattg cggcgaaagc
    15841 aattagtcga atgaaattag gacaagttga tgaaataact actgccaata ttggtaagtt
    15901 tcatggcggt tcagctacga atattgttgc agatgaagtc attttagaag cagaggcacg
    15961 ttctcacgat ccagaaagaa taaaaacaca agtcaaacat atgactgatg tgtttgaaac
    16021 tactgcatca gaattaggtg ggaaagcagt agttacagtg gagcaaagtt atccaggatt
    16081 taaaattaat gataatgaag cggttgttaa aattgcacaa gaaagtgctc gtaatttagg
    16141 attatctgca aatacaatta tttctggtgg cggttcagac ggcagtatta ttaatacatt
    16201 tggtattcct tcagtcattt taggagtggg ttatgaaaaa atccatacaa caaatgagag
    16261 aatgccgatt aaatcattga acttgcttgc aagtcaagtg ttagaaataa tcaaaatagt
    16321 tgcgcgacat tcgaaatgat ttagtgtagt gtatgtctat tttgtgatac aatactaatg
    16381 aaaaatcaac aagtgaggta agtaatatga cacaacaaat tggagttatt ggtttagccg
    16441 ttatgggtaa aaacctagct tggaatattg aatcacgcgg atatagtgta tctgtattta
    16501 accgctcaag tgaaaaaact gatttaatgg ttgaagaatc aaaagggaaa aatattcatc
    16561 caacgtattc attggaagaa tttgttaatt ctttagaaaa accacgtaaa attttattaa
    16621 tggttcaagc aggtaaagct acggacgcaa cgattgatag tttgttacct ttattagatg
    16681 atggagatat tttaattgat ggtggtaata caaactatca agatactatc agacgtaata
    16741 aagcattagc acaaagtgca atcaacttta ttggtatggg cgtttctggt ggtgaaattg
    16801 gagcgttaac tggtccgtca ttaatgccgg gtggacaaga agaagcttat aacaaagttg
    16861 cagatattct tgatgcaatt gctgcaaagg caaaagacgg tgcttcatgt gttacttata
    16921 ttggacctaa tggtgctgga cactatgtga aaatggtaca caatggtatc gaatatgctg
    16981 acatgcaatt aattgcagaa agttatgcta tgatgaaaga attattaggc atgtctcatg
    17041 aagacattgc tcaaacgttc aaagactgga atgctggtga attagaaagt tatttaattg
    17101 aaatcactgg tgatattttc atgaaattag atgaaaataa agaagcatta gttgaaaaaa
    17161 ttcttgatac tgcaggtcaa aaaggtacag gaaaatggac ttcaattaac gcattagaat
    17221 taggtattcc attaacaatc attactgaat ctgtatttgc acgttttatc tcttcaatca
    17281 aagaagagcg cgtaaatgct tctaaagaat taaatggtcc aaaagcatca tttgatggtg
    17341 acaagaaaga ctttttagaa aaaattcgta aagcattata tatgagtaaa atttgttctt
    17401 atgcacaagg tttcgcgcaa atgcgtaaag caagtgaaga taatgaatgg aatttaaaac
    17461 ttggtgattt agctatgatt tggagagaag gttgcattat ccgtgcacaa ttcttacaaa
    17521 agatcaaaga tgcttacgat aataatccag gattacaaaa cttattgcta gatccttact
    17581 ttaaaaacat cgttactgaa tatcaagatg cgttacgtga tgttgtcgca actggtgttc
    17641 aaaatggtgt accaacacct ggtttctcat caagtattaa ttactatgat agttaccgtg
    17701 ctgctgattt acctgcaaac ttaattcaag cacaacgtga ctacttcggt gctcatacgt
    17761 acgaaagaaa agataaagaa ggcgtattcc atacacaatg gattgaagaa taatattgat
    17821 taaaggatag ttgaaagata ctatcctttt ttatatcttt tgataaatat aaaagagagt
    17881 gtgatagagt agctacaacc tataaaaaat tacacaagta ttatccttga ccttaaaagc
    17941 atcccgccat atttttaagc attttgtgaa gtaataaaat atttactgtg ttatgatagc
    18001 atgtactata gagaaataga ggtactattt tatggaaaag ttaaatcaag ttatgttata
    18061 tgttgatgat gtcgaaaaag cgaaagcatt ttggacagaa actttagaat ttgttgttgt
    18121 aagtgaaaca ccgttagctg aggactatgt tgcagttgaa gtatcaccta ccaaagatgc
    18181 tgaaacgtca ttgacgatta tggcaaaaga atttattgaa aagcatagcc cggaagtgaa
    18241 cctaggaaca ccatctttaa tgtttaaaga gaaaaacttt gatgcattat actcaaaatt
    18301 aaatgattta ggtttaactg ggcatgatat agtagaaatg aatggtcaac gtgtatttaa
    18361 ttttcaagat ggccaaggta actattttgc agttagtgac taaacaatcg attttaatat
    18421 ttatacaaac ctttatattg attttcggat tgaatttcag tatgaaggtt ttatttttat
    18481 cgttttatgc aatcgtttgc acaaatcttt tgactttttt agaaagtgtg actaaactgg
    18541 tattatacag tggttggaag cgttttccaa tgcagaaatt aacaggaggt gattaaatgg
    18601 ttacgattaa agatgttgca ctaaaagccg gtgtttctcc ttcgacagtt tcaagagtta
    18661 taaaaggaaa tcaacgtatt agcgaagcga caatttcaaa agtgaagaaa gttatggaag
    18721 aattgaatta ttttcctaat accgctgcta gaactttaat tacaaaccaa acatataaaa
    18781 ttggtttagt gttaaaaggg tctgaggagc ctattcgact gaatccattc tacatcaatg
    18841 tattgctagg aatttctgaa acgtgtaacc agcatggcta tggtacacaa acgacagtct
    18901 caaataatat gaatgattta atggatgaag tttataaaat gattaaacaa cgaatggttg
    18961 atgcgtttat actgctctat tcaaaagaaa atgatccgat taaacaaatg ttaattgatg
    19021 aaagcatgcc atttattgtg attggtaagc ctacatcgga tatagatcat caatttacac
    19081 atatagataa tgataatata ttagcttctg aaaatttgac acgacatgtt attgaacaag
    19141 gtgtagatga attaatattt attacagaaa aaggaaattt tgaagtttca aaagatagaa
    19201 ttcaaggatt tgaaacgata gcttctcaaa ataaaattaa ttatcaaatt attgagacta
    19261 gtaatgaaag agaagttatt tttaattaca tgcaaaatct acatacgcgt ttgaaggacc
    19321 caaatattaa acaggcaatc atttcgttag atgctatgtt acatttagcg attttaagtg
    19381 tcctatatga acttaatatt gaaattccga aagatgtaat gacagcaacg ttcaatgatt
    19441 cttatttaac cgaaattgcg tcaccaccac aaacctgtat tgacatcaag cctcgaatgt
    19501 taggtcaaca ggctggtgca gcaattttaa atatattgaa aaacaaagca caggatgtta
    19561 ttgaactcgt cattatagat acagaattaa aaataagaaa atcaacacag cgatagcaaa
    19621 ggagttttaa aatgaataag caatggtgga aagaagcagt agcatatcaa gtatatccaa
    19681 gaagttttaa tgatagtaat cacgatggta ttggggattt acctggaatg attgataaat
    19741 tggactactt aaaagatttc ggtatcgatg tcatttggct cagtccaatg tttaaatcac
    19801 ctaatgatga caatggttat gatattagtg actaccaaga gattatggat gaatttggaa
    19861 cgatggaaga ctttgatcgt ttattaaaag gtgttcatga tagaggcatg aagcttattt
    19921 tagatttagt tgtaaatcat acatctgatg aacatccttg gtttatagaa tccaaatcta
    19981 gtaaagacaa tcccaaacgt gattggtaca tttggcaaga tccaaagcca gatggctctg
    20041 aacctaacaa ctgggaaagt atatttaatg gatctacatg ggaatatgat gctaatactg
    20101 agcaatatta tttccattta ttcagtaaaa aacaacctga tttgaattgg ggtaatccgg
    20161 aagttagaga tgctgtattt gaaatgatga actggtggtt tgataaaggc attgatggat
    20221 ttagagtaga tgcaattacg catattaaga agacgtttga agcgggtgac ttacctgtac
    20281 ctgagggtaa aacatatgcc ccagcatttg atgtagatat gaatcagcca ggtatacaaa
    20341 cttggttaca agagatgaaa gatcgctcat taagtaagta tgacattatg actgttggtg
    20401 aagcgaatgg tgtaagccct gatgatgctg atgactgggt cggggaagaa aatggtaaat
    20461 ttaatatgat attccaattt gaacatttgg gactgtggaa tagtggtgat tctcactttg
    20521 atgtaaattc gtataaatct gtattaaata gatggcaaaa acaacttgaa aataaaggtt
    20581 ggaatgcgtt gtttattgaa aatcacgacc aaccacgacg tgtatcgacg tggggtgacg
    20641 atgacaagta ttggtatgaa tcagcaacaa gtcatgcagc tgtttatttc ttgcaacaag
    20701 gtacgccatt catttatcaa ggtcaagaaa ttggtatgac gaattatcca tttgaaagta
    20761 ttgaaacgtt taacgatgtt gctgttaaaa atgactatca aatagtgaaa gctcaaggtg
    20821 gagatgtaga cgctttactt gcgaaatata aagatgagaa ccgagataat tctcgcacac
    20881 caatgcaatg ggatgatacg ttaaatggag gatttacaaa tggtgaaccg tggttcccag
    20941 tgaatccgaa ttataaaact atcaatgttg cacaacaatt agaagatgag cattcagtat
    21001 tacaatttta taaagattta attcaattaa gaaagtctaa tgatgtatac gtatatggtc
    21061 aatttgattt agtagatgct gaaaattcac aagtttttgc gtacacgaga acattaaatg
    21121 aaaagcaagt tcttattgta ggtaatctta ctaaccacga agcagaatta actgtaccat
    21181 ttgatttaag ccatggagaa gtaaagctat ttaattatga tgccaaagtt aatttaaaac
    21241 agttacgtcc atatgaagca tgtgttatcg aactaaatta acatattaat atgcccccta
    21301 atagaatcta aggccatttg tagacctata gatatattag ggggcttctt tgttttttat
    21361 tatttaatct tcgtcataag gactctgttt aacaggaatc caaagctgaa ttttagtgaa
    21421 tgggtcatta aatgaaatat cgagagggta cacttcaaca tataaatcat ttcgttcata
    21481 tggcaatgtt aactgcaaac ttgattcaat atagtaccaa gcttcattag ttgcataatc
    21541 aatttcacct tgtaaattga atttcgcaca atgtttgcca ggtaaaaatc gactttctaa
    21601 gtgtgcagga taacgttcac ttggtacacc tacaaatatt tctaaaccat tttcaagagg
    21661 acaactaata acaaatagtt caaatggact aacgtcatta tatcgtcgta actctttaat
    21721 tttaccatca atgagcaagt cttctaaaaa atccggaaca ttaaaaggat gtgacaaata
    21781 cttagtgtct ataaatcgtg catatccaac caatgaaata tcatctgtct cttctaatct
    21841 gtatggataa ggtgctctct cagtagttga taatttgata tataatcgct cttgaatttg
    21901 taattcatct tttttagtag aggcttgaat aggtgatacg ccgtgaaaat cactaaaatc
    21961 atttgcaaaa tcatttgaat ttgcatagtg atatttttta gcgatatcta caagtcgtgt
    22021 agcaccatta atcacatcat ttgcagcgag tgtcatttta cgcgcgcgtg cataagcttc
    22081 tggagataag ccgacaatca ttttaaatga ttgatcaaga tggtatggcg aaagaccaac
    22141 gtaatcactt aattcttgca aattgaaagg ctctaataaa cgatcttcaa tataaacaat
    22201 tgcctgttgt atttgcttga taacgtccaa aactttcact ccaattaatt caatcattta
    22261 tttttattct acattatttc tataaattat acacccattt gttcaatgat tattaaaata
    22321 gttttgggca ttgtaaaata taatttcata atatagtcta gaaaaaaagc gaatgataga
    22381 acaattgatt tacttgattc gtaatcaatc cttgtcattc gctcatttat ttttgtttaa
    22441 catgtgcgtt ttaattcaat tattgaatat cgtcccacca atggttacca tcacgagcaa
    22501 gtagtaaatc actttctaat ggaccattag tacctgattc atagttaggg aattctggat
    22561 caaccatatt ccattcatct tgaattgcat caacaaattt ccatgttgat tttaattctt
    22621 cccagtgcgt gaagttagtg gcatcacctt taagacaatc aaataataga ttttcatatg
    22681 catctacagt attcatttta tcttgagcgc tcattgagta agacaattgg acaggttctg
    22741 tttcgatacc ttgtgtattt ttcttagcat ttagatgtaa agatacacct tcattaggtt
    22801 ggatattgat tactaatagg tttgaatcta acagtttatc agtttcatag tataagttca
    22861 ttggtacttc tttaaattca acgacaactt gaattgtttt agatttcata cgtttaccag
    22921 tacgaatata gaatggtaca ccagcccatc taaagttatc aattgttaat ttacctgaaa
    22981 caaaggtagg tgtgttagag tcatctgcaa cgcgatcttc atcacggtat gctttaactt
    23041 gtttaccatc gatatagcct tcgccatatt gaccacgaac aaagttcttt ttaacatctt
    23101 cagattggaa atgacgcagt gatttaagta cttttacttt ctcagcacgg atatcttcac
    23161 tatttaaact aataggtgct tccatagcta ataatgcaac catttgtaac atgtggtttt
    23221 gcaccatatc ttttagcgcg ccacttgatt cataataacc accacgatct tcaacaccta
    23281 gtatttcaga agatgtaact tggatgtttg aaatatattt gttattccat aatggttcaa
    23341 acatcgcatt cgcaaaacgt aatacctcga tattttgaac catgtctttt cctaaatagt
    23401 ggtcaatacg ataaatttct tcttctttaa atgatttacg aatttgattg tttaatgctt
    23461 cggctgattt taaatcacta ccgaatggtt tttcgataac aaggcgttta aatccttttg
    23521 tatcagtaag accagaagat tttagataat cagaaataac gccaaagaat tgtggtgcca
    23581 ttgctaaata gaatagtcga ttacctttta attcaaattg gctatctaat tcattactaa
    23641 aatctagtaa ttcttgatag ctttcttcat tactaacatc atgtctatga tagaagacat
    23701 gttccataaa cgcgtcaatt ttgtttgtat cttttacgtg cttttgaatt gatgatttta
    23761 cttgattacg gaaatcatca ttagtaatgt cacgacgtcc aataccgatg atggcaatat
    23821 gttcatctaa attgtcttgt tggtagagat ggaatattga tggaaacaac ttacgatggc
    23881 ttaagtcacc agttgcacca aagattgtga ttaaacatgg gatgtgtttg tttttagtac
    23941 tcaagattaa aacctcaatt cttaattaga tatatgattt attattataa gataatccat
    24001 tcagtaggtc atacaatatg ctcgactgta tgcagtgtct taaatgaaat atcgattcat
    24061 gtatcatgtt taatgtgata cttattaatg ataagtataa cgtaattatc aaaatttata
    24121 tagttatgtc tacgttaaag ttagaaaaat taactagcaa agacgaattt ttaacagatt
    24181 ttgattcaag tataaattaa aactaaattg atacaaattt tatgataaaa tgaattgaag
    24241 aaaaggaggg gcatatatgg aagttacatt ttttggaacg agtgcaggtt tgcctacaaa
    24301 agagagaaat acacaagcaa tcgccttaaa tttagaacca tattccaatt ccatatggct
    24361 tttcgacgtt ggtgaaggta cacagcacca aattttacat catgcaatta aattaggaaa
    24421 agtgacacat atatttatta ctcatatgca tggcgatcat atttttggtt tgccaggatt
    24481 actttctagt cgttcttttc agggcggtga acagaagccg cttacattgg ttggaccaaa
    24541 aggaattaaa gcatatgtgg aaatgtctat gaatttatca gagtcacatt taaactatcc
    24601 aattacatat attgaaatcg atgatcatct gacatatcat catgatggtt ttactgtaga
    24661 ggcgcattta cttaatcatg gtattccatc atatggatat agagtaatgg cacctgaaac
    24721 tacaggtaca atcaatgttg aagcgttaaa aaacattggt cttgaaccag gtcctaaata
    24781 tcaagaagta aaatctcatg atacatttga acataatggt caagtttatc aatcaaaaga
    24841 ttttagaggt gagtccaaac agggtccagt tgtagcaatt tttggtgata caaaaccatg
    24901 ttctaatgaa cgtgtgattt ctagagatgc tgatgtgatg gtacatgaag cgacctatat
    24961 agatggtgaa aagcatttag ccaacaatta tcatcatagc catattgaag acgtatttgc
    25021 attgataaaa gaagccaatg ttaaacgtac gttgattaca catttgagca atcgctataa
    25081 tacagaagat ataaatgaaa tttatcaaac attgatacaa aatgaagata cacctaattt
    25141 taattttgta aaagattttg atagttttaa aatctaatga caaagtaata aacaatgctt
    25201 gccaaataac aataaattat aaaaataacc cgtcaaacac agtgcttgcg atgatgcata
    25261 catgtgttgg cgggtttgtt tttattggtc ttctatatta gaaagttcaa tactacggtc
    25321 gacggcagcg tttagacaat cttcgaaaat agatactaaa tcatattgtg acaatgtatc
    25381 aaggccagct tgtgtcgtac cacctttaga ggtaatattt tttcttaatt gagccatgct
    25441 caaatctgaa cgttcaatca tcttacttgt acctataata aggttgcgta tagattcttc
    25501 aacttgttct ttttctagac caagtttcgt accagcttta acatattgct cgaatacatg
    25561 atataaaaat gctgggccgc ttccggtgat agctgttact tgatgtaaat gatcttctga
    25621 tacttcaatt acagaaccaa atgctttaac taaatcgtta atttcatctt tagatttagg
    25681 gtcaaagttg tttgaaaaac taatgccagt aacagagtgt ccaacttgcg catttgtgtt
    25741 tggcataatt ctagcaactg gattttggca ttctaattgt tgtttaatat aatcaatcgg
    25801 aatacctgcc ataattgaaa tgaagcaatt gtcttttgta atatgtggtt tgatgcgtgt
    25861 tgctagagca tcaaaatcat gtggtttggt acctaaaaat acataatctg catcttttaa
    25921 taatgtcgca tcatcataac tatagttaac acctagtttt tcagcgaatg cttttaaagc
    25981 ttgttcatta gatttatttg ttaaatatat atcattggca tctaagttgc ttgagttaat
    26041 aattcctgta aatatagctt gtgccatatt accagctcca taaaatacga gtttcattaa
    26101 cgtcacatcc ctttagtttt tgatttgtat gttatatttt cttattattg tatatatatt
    26161 aggaaagata atcaacttgt ctcaattatg taaagttgat aaattgcatt ctatataatt
    26221 gaaaaggaag gttaattgat gaaaggacaa cattttatag tgactggtgg tacgagtggt
    26281 ttaggtttat cgattgtccg aaagttacta gaaaataaag tgcatgtaac gttgttggtg
    26341 cgtgatgttg ataaagcaac tagaatcttt gaacaggaat tggggaaaac tataaatgtt
    26401 attccttgtg acttaaatga tttgaagtcg atacaggcac tgcaatttga agacaataca
    26461 tcattcgatg gattcattta tagtgcaggt ttaggttatt tcaaatctat ttcagaccat
    26521 agtttttcag aaatgattga aacgtatcag cttaatttaa ttagttttaa tgttttatat
    26581 actgtgttga gaccatattt aacatctaac gcacatattg taggtatttc aagtcaagct
    26641 gcattttcca cacaagcaaa tgctgcacat tatggggcat cgaaagcagg gttttatgca
    26701 ttaatgaatg ctttaagact agagtcacca aacttacata tcatgacagt taatgttggc
    26761 ccgattgata caccttttca tcaaaaagcc gacccatcaa tgaagtatgc taaaaagatg
    26821 ggggaaatta tgcttgatgc taatcagctt gcagaagata tcatttatgg cattaaaaca
    26881 aaacaattgg aaattaatcg gcctaaatgg atgcatcatg cgttgaaaat gtatcaaatt
    26941 gcgcctagat tttttgaacg atgtttccct aaattattta aaaataaagc ataaataggt
    27001 gggatggcaa tgaactgtca atcggagagt gtggtaagac tttgtgtacg atatgcggaa
    27061 caactatcag tatttgaaga atttacagtg ctggatattt taggcgatat ttctgtagac
    27121 caaatttcag acggtacatt atattatact tgtgaaaaat tcaaactact tgtgttgcaa
    27181 ggaaatgtgc tgggtgtaca aataataact aataatgatg aatcaacatg cgaagtgaaa
    27241 tatagaaaga tgttttaata aaattattaa gagatgaagt ggcagaagtt tgcttaaggc
    27301 aatgccgcaa ggcgaaatta ctaaatactg atgcgtgagg acaacgcttt aatttctatc
    27361 ataagtgagt aatcataatc ataaattata gcgaattgta cataaacaac agcagtaagg
    27421 catttttata atgaaacgcc tcatgctgct gtttttatta ctgaaaatga cttctacact
    27481 aaatgattgg tatattccaa atcctttaca cgagctcttg ctgttttaat ctgatctaaa
    27541 ctaactttag tatgatagtt ttcaatattt tcttttaatt ggtcgacgct acttgcacct
    27601 acaatgatgg aaccaagcac gtcatgtgat gttaaataac taaatgtcaa tgcagataaa
    27661 ttactttcaa tttctttaat agaggctatt gtttcaccca attcatcatg agaataatca
    27721 aaaataccat ctttaaattt attgtcgagc acattaacac tgtttgaagt taataatcct
    27781 ttagacacag gtcctcttgc caatacttta acatcattgt tgtgaattgc atctaataat
    27841 gattctggac gattatcaat caaattgaat tgagacatta tcgtttcgat ttgactatgt
    27901 tttaaataat aatcaattac atttgggcga atagaagaaa taccgtaagc acgtataatt
    27961 ccttcttgtt tcaattcatc aaatgcgctt attgtttcgt ctaatgggtc atcaatggtt
    28021 ccgccatgaa gttgatataa atcgatatga tcgataccta aacgctttag tgaaccttta
    28081 actgcctctt taatatagga tttactcgga tcccatgttg tactgccatc ttttgttaaa
    28141 cgattgccta cttttgtacc aataaaaata tcatcgcgtt gttgatattt aagaagtgcc
    28201 ttaccaacaa ctgattcatt tataccttta tcgtacatat ctgctgtatc aaaataagtg
    28261 ataccatttt caacagcaca atctataatt tgttcggctt tttttaaatc tgtacctaaa
    28321 ctcatgcaac ctagacctaa ttcagataat gaaataccac tttttaatat atttttttgc
    28381 atgtctgttc tcctttcgat acactttagc taacaagcat agtgtatcaa tatataatat
    28441 gaatttaaag gaacgtgatt ataatggatt taaatgaaaa aacaattgat cgaacagtta
    28501 tttataatgg caaaattgta gatgtagaaa ttcatacagt gacattacca aacggtgaaa
    28561 cgtcaacaag agaattagtt tatcataatg gtgcagttgc tgtttgtgca gtgacaccta
    28621 aaaaagaagt cgtattagtg aaacagtatc gtaaaccagt agaaaaacca ttgctagaaa
    28681 ttccagctgg taaattagaa gatgatgaag atagagtcga agcggcaaaa cgtgaattag
    28741 aagaagaaac aggatatatt gccaaagagt taacacatgt tgtagatatg tatggttcac
    28801 caggtttttg cgatgaacaa ttatcaatat attttacgga taatttagaa gaaggtacag
    28861 ttcacttaga tgaagatgaa tttgtcgaag tcattaaagt tccgattgag aatgttaaat
    28921 cgatgctaat gaataaagaa atagaagatg cgaagactat cattgcattg caacacctat
    28981 tattaaatta taatcattct aaataacaaa tgagaaaagc ttgcatttta ttgagaaaac
    29041 tgttagtttt aattgtgaag tttgaaataa tttgtaatga ttttaattat tagtagggga
    29101 gtggacatcg ttggaagaac gattaaatcg cgttaagcaa caattacaac aatcatcata
    29161 taagctaacg ccacaacgcg aagctactgt tagagttcta attgaaaatg aaaaagatca
    29221 tctaagtgct gaagacgtat atctgaaagt aaaagataaa gcgcctgaaa ttggcttggc
    29281 gacagtatac agaacgttag agttgttagc tgaactaaaa gttgtcgaca aaattaactt
    29341 tggtgatggc gtcgctcgtt ttgatttaag aaaagaaggc gcaaaacatt tccaccatca
    29401 tttagtatgt atggaatgtg gtcgtgtaga tgaaatcgat gaagatttgt taccagaagt
    29461 tgaaaatcga gttgaaaatg agttcaattt taaaatttta gatcatcgtt taactttcca
    29521 tggtgtgtgt gaaacgtgcc aagctaaagg taaaggatag taaattgcgt aggttaaatt
    29581 aaccttcgct ttttttagag gtgtggttat ggaaacaatt attgaagaat atttgcgttt
    29641 tatacaaatt gaaaaaggac taagttctaa cacaattggt gcttatagac gtgatttgaa
    29701 aaagtatcaa gattatatga ctgaacatca tatctcgcat attgatttta tagatcgaca
    29761 attaattcaa gagtgtttgg ggcatttaat agaccaaggg caatctgcta aatctattgc
    29821 gcgatttatt tcaacaatcc gtagttttca tcaatttgct ataagagaaa aatatgcggc
    29881 gaaagatcca acggtattat tagattcacc aaaatatgac aaaaaattgc ctgacgtttt
    29941 aaatgttgac gaagtgttgg ctttattaga aacgccagat ttaaataaaa ttaatggata
    30001 tcgtgatcgt acgatgttag aacttctgta cgcaacggga atgcgtgtat ctgaattgat
    30061 acatttagag ttagaaaacg tgaacttaat aatgggattt gtacgcgtat ttggtaaagg
    30121 cgataaagaa agaattgtac cattaggcga cgcagtcatt gagtacttaa ctacttatat
    30181 tgaaacgatt agaccgcaac ttttaaaaaa gactgttact gaagtcttat ttttaaatat
    30241 gcatggtaaa cctttatcac gacaagcaat atggaaaatg attaaacaaa atggtgtaaa
    30301 ggcaaacatt aaaaagacgt taacgccaca tacgttacgc cactcttttg cgacacattt
    30361 attggaaaat ggcgcagatt taagagcagt gcaagagatg ttaggtcact ctgacatatc
    30421 tactacccaa ctctatacac atgtttcgaa atctcaaatt agaaaaatgt ataaccaatt
    30481 tcatcctaga gcataaagtg aacaataact caaaagtcac aatacacatg actaaaaatg
    30541 tctgtgctat tgtggctttt ttaaattggt tgattaatta cgtctatgtt ttcttaattg
    30601 aatcgcttct tcttttgctg caatcacttc tgaacgatca cggcgcatgt gatggtctac
    30661 aataaaagga tctgttgctg tttcctgatt ataatcatag tctggatagt tggccttgat
    30721 gatgcgttca aagactggag ttattggtaa tataacagat gaaaaaggct ttgctgcatt
    30781 caattttgca atctgttgct caattaacaa ctgataatca tttaaattaa ggtataacgc
    30841 atctctatct ttagcatttt gtattatttc tttagattta ttaaaagact tataggcgcc
    30901 ttttaaatta ttgcggcgat aatggtaaca agcagttgca aacaagatta aactaacaac
    30961 tgcatcttgc ttactgtagt tattttcagc tttccatgca tcttctaaaa tgtcatgaca
    31021 taggaaataa tgttgcttag tatgaaattg ataatagaaa tttatcagtg cctgttgcat
    31081 tttgttatca ccccaattta aaagtaagtt attttcatgc tataatattt tagagaatta
    31141 tgcacatatg acgcaatacg aggtagatat tatgtatgaa gttaaattag atgctttcaa
    31201 tggaccatta gatttattgc tgcatcttat ccaaaaattt gaaatagata tttatgatat
    31261 tcctatgcaa gcattaacag agcagtatat gcagtacgtt catgcaatga aacagcttga
    31321 aattaatatt gcaagtgaat acctagtatt agcgtcagaa ctcttaatga ttaaaagtaa
    31381 gatgctatta ccacaatcaa catcagatat ggatgttgat gatgacccac gggaagattt
    31441 agttgggcgt ttaatagaat atcaaaatta taaagaatat actgctattt taaatgacat
    31501 gaaagaagaa agagattttt attttacaaa aagaccgaca gatttatctc atttggaaac
    31561 agatgaatct tgggatccaa atcatacgat tgatttaact gaattaattg tagcttatca
    31621 aagagttaaa aatagagttg agttaaatac acctaaatct gttgaaatcc gaaaagagac
    31681 atttaccatt caacaagcta cagaacaagt gacatcgaga ttgaaagata aagatcattt
    31741 taacttcttt agtctgttta cgttttctga gccaattgaa caagtagtca ctcacttttt
    31801 agctatttta gagatgtcaa aagcaggaat aattaatatt gagcagcaac gtaattttga
    31861 agatattaac attattagag gagtgaacta ccattttgga taatcatggt atattagagt
    31921 cgcttttatt tacagctggc gatgaaggtt tagatgaaaa acaactatta gaaatattag
    31981 atatgtcgaa agaccaactc gttgaattaa ttgaaaatta ttcatcacat ggattaatga
    32041 tacaacgatt tggaatgacg tatgttttaa cgactaaaaa agaagcggca acgtatattg
    32101 aacaattaat tgaacaaaag tcacaaatga aattatcaca agcagcaatg gaagtactat
    32161 caattattgc ttataaccag ccattatcaa gaagtgatat tgaattaatt cgtagtatca
    32221 attcagatgg tgcagttaag acattgattg ccaaaggact agttgaggct aaagtggtta
    32281 atgaacagcg tagccaacag ttaattacta ctgatttatt tttaaatgta tttggtattt
    32341 ccaatataga agatttgccg acaactgaag aagacgatga agaaatggat gcttttttca
    32401 gtaatctagt caatcaaaaa ggagaaaata atgactaaag aattagaacg attacaaaaa
    32461 cgtatagcta atagcggtta tacttcaaga cgtaaggcag aaactttaat ttctgaagga
    32521 aaagtgaaag tgaatggtac gactgtcact gaattaggaa caaaagtgaa gccatcagat
    32581 acgattgaag ttgaaggtat taaaatagaa ctagaagaca aaatttacat tttattccat
    32641 aaaccaactc aagtgataac aagtgtatct gatgatagag gacgtacagt ggttacagat
    32701 tactttaaag atatagaagc acgtatttat cctgttggtc gtttagacta tgatacttct
    32761 ggattgttat tactcacaaa tgatggtgaa tttactaatt taatgacaca tccaagatat
    32821 caaattaaga aaaaatatgt tgcgaaatta aaaggttatt taatgagaga agaagtgaaa
    32881 gcgctagaaa aaggtattga attagaagat ggtatgacgc aaccggctga agtgaaagtt
    32941 aagaaacaag ataaagataa aaacacaaca ttggttgaaa ttacaattac agaaggtcgt
    33001 aatcgccaag tcagaagaat gttcgaacat tttggtcatc aagtgacgaa actgtctcgt
    33061 attgaatatg ggcctttaaa tgttgtcggt ttgaatgctg gtgaaggacg tgtattgacg
    33121 cctcatgaag taaaagtgat gcgtcattta gcagaacatg ggaaataatt aaataaaata
    33181 tgtatttatc acaaagtttg agaatttttt cacaaaattt taggttttta ataactgtat
    33241 gcgctttcct gtgatataat acttttgtaa agaaaagcat gtgtgggagg tatgacctgt
    33301 atgtcgaacg aaatacttat cgtagatgat gaggatagaa tcagaagatt acttaaaatg
    33361 tatttagaaa gagaatcttt tgaaatccat gaagcaagta atggccaaga ggcttatgaa
    33421 cttgcaatgg agaataatta tgcttgcata ctactagatt taatgttgcc tgaaatggat
    33481 ggtatccagg tggcaactaa attgcgtgaa cataaacaaa caccgattat tatgttgact
    33541 gctaaaggtg aagaaacaaa ccgtgttgaa ggttttgaat ctggtgcaga tgattatatc
    33601 gtcaaaccat tttcaccaag agaagtagtc ttaagagtta aagcacttct aagaagaacg
    33661 caatctacaa ctgtagaaca aagcgaacct cacgcacgtg atgtgattga atttaaacat
    33721 ttagaaatag ataatgatgc acatcgcgta cttgctgata atcaagaagt taatttgact
    33781 cctaaagagt acgaattatt aatatattta gctaaaacac caaataaagt atttgaccgt
    33841 gaacaattat taaaagaagt ttggcattat gaattctatg gtgatttaag aacagttgat
    33901 actcatgtta aacgacttag agaaaagtta aatcgtgtgt ctagtgaagc tgcgcatatg
    33961 attcaaacag tctggggcgt tgggtataaa tttgaggtta aatctaatga tgagccggct
    34021 aaatagtgtc gtaattaaac tgtggttaac tattatttta atagtgacga cagttttaat
    34081 tttattaagt attgctttaa ttacctttat gcaatactat ttcacacaag aaaccgaaaa
    34141 tgccataaga gaagatgcta gacgtataag ttcactggtc gaacaatcac ataataaaga
    34201 agaagcaata aaatatagtc aaacattaat tgaaaatcct ggtgggttga tgattataaa
    34261 taataaacat cgtcaatcaa cggcttcact ttctaatatt aaaaagcaaa tgttgaatga
    34321 agtagtcaac aacgaccatt ttgacgatgt gtttgataaa ggtaaatctg ttactcgaaa
    34381 tgtaacgatt aaagaaaagg gctcatctca aacatatatt ttgttaggct atccaacaaa
    34441 agcacagaag aatagtcata gcaaatatag tggagtcttt atatataaag acttgaaatc
    34501 aatcgaagat acaaataatg ctattacgat tatcaccata attacggctg ttattttctt
    34561 aacaattaca acagtctttg cgtttttctt atcgtcaaga attacaaaac ctttaagacg
    34621 tttaagagac caagctacac gtgtatctga aggggattac tcttataaac cttctgtcac
    34681 aacgaaagat gaaattggtc aattatcgca ggcatttaat cagatgagta cagaaatcga
    34741 agagcatgtc gacgcattat ccacatctaa aaatattaga gacagcttaa ttaactctat
    34801 ggtagaaggt gtcctaggta ttaatgagag tcgacaaatt atcttatcta ataagatggc
    34861 gaatgatatt atggacaata ttgatgaaga tgctaaagct ttcttattaa gacaaataga
    34921 agatactttt aaatcaaaac aaactgaaat gcgcgattta gaaatgaatg cacgattctt
    34981 tgttgtgacc acaagctata tcgacaagat tgaacaggga ggtaaaagtg gtgttgttgt
    35041 gacagttcgt gatatgacta atgagcacaa tctagatcaa atgaagaaag atttcattgc
    35101 taatgtatca catgaattac gtacaccgat atcattactt caaggttata ctgaatcaat
    35161 tgtagatggt attgttacag aaccggatga aataaaagaa tcgcttgcca ttgtccttga
    35221 tgaatcgaaa cgtttaaatc gtttagttaa tgaattgtta aatgtcgcac gcatggatgc
    35281 tgaagggtta tccgtaaata aagaagttca gcctattgca gcgttactag ataagatgaa
    35341 aattaagtat cgccaacaag ctgatgattt aggtctaaat atgactttta attattgtaa
    35401 gaagcgtgtt tggagttatg atatggatcg catggaccaa gtactaacga acttaattga
    35461 taatgcatca cgttatacga aacctggaga tgaaattgca attacttgtg atgaaaatga
    35521 aagcgaagat attttataca ttaaagatac aggtacaggc attgcaccag aacatttaca
    35581 acaagtattt gatcgttttt ataaagttga tgcagcgaga acgcgaggta aacaaggtac
    35641 cggtttaggt ttgttcattt gtaaaatgat tatcgaagag catggtggtt ccatagatgt
    35701 taaaagcgaa ttagggaaag gcacaacatt tattattaaa ctaccaaaac cagaataaaa
    35761 ttgaatatag ttatttcaga acgcatgtta ttgattagag actctaattt atagcatgcg
    35821 ttttttgatt gatgtgggga attttgtatg tggattagaa cttagggttt ttgcgaatat
    35881 caactattaa atatattact aatttataca aaaatataaa gtttgataaa gttatttatt
    35941 tgattataaa aatagggtaa aatatagata tattgtatta attaaattat tcgaggtgtc
    36001 atatgaaaaa attcattgga tcagttttag ctacgacatt aattttaggg ggatgttcca
    36061 cgatggaaaa tgaatcaaaa aaagacacaa aaacagaaac aaaatctgta ccagaagaaa
    36121 tggaagcttc aaaatatgta ggccaaggct ttcaaccgcc tgcagaaaaa aatgcgattg
    36181 aatttgcgaa gaagcatcgt aaagaatttg aaaaagtagg tgaacaattc tttaaagata
    36241 actttggact aaaagttaaa gctacaaatg ttgtaggtaa agatgatggt gtagaagttt
    36301 atgtgcattg tgaagatcat ggcattgtat ttaatgcaag tctacctttg tacaaagatg
    36361 ccatccatca aaaaggatca atgcgcagta atgacaatgg tgatgatatg agtatgatgg
    36421 tgggtacagt gctgagtggc tttgaatatc gagcgcaaaa agaaaagtat gataacttat
    36481 ataaattctt caaagaaaat gaaaagaaat atcaatatac aggctttaca aaagaggcaa
    36541 ttaacaagac acaaaatgtc ggatataaaa atgaatattt ttatattaca tactcttcta
    36601 gaagtttaaa agaatatcga aagtattatg aaccactgat tcgaaaaaat gataaagaat
    36661 ttaaagaagg aatggaacga gcaagaaaag aagtgaatta cgctgcaaat acagatgctg
    36721 ttgctacact tttttctact aagaaaaact ttactaaaga caatacagta gatgatgtaa
    36781 tcgaactaag tgataaatta tataatttaa aaaataaacc agataaatct acaatcacaa
    36841 tacaaatagg gaaacccact attaatacta agaaagcctt ttatgatgat aatcgtccaa
    36901 tagaatatgg ggtgcacagt aaagatgaat aaaattaatg atagggattt aacagaatta
    36961 agtagttact gggtttatca aaatattgat ataaaaaaag aatttaaagt taatggaaaa
    37021 aggtttaaac aagtagacag ttataatgat gataagaata gtaatttgaa tggtgctgct
    37081 gatattaaaa tatatgagtt attagatgat aaaagtaaac caactggtca acagacaata
    37141 atttatcaag gaacatctaa tgaggcaatt aatccaaata atccattaaa atcatcgggg
    37201 tttggagatg attggctcca aaatgctaaa ttaatgaata atgataatga aagcacagat
    37261 tatttaaagc aaacagatca attatcaaat caatataaaa taaagttaga agatgcagat
    37321 agattatcaa atagtgattt tttaaaaaaa tatagaatgg aatcaagtaa cttcaaaaac
    37381 aaaaccattg tggcggatgg cggtaattcg gaaggcggtg caggagcaaa atatcaagga
    37441 gcgaaacatc cgaatgaaaa agttgttgct actgactcag caatgattcc ttatgctgct
    37501 tggcagaaat ttgctagacc acgctttgat aatatgatta gttttaatag taccaacgat
    37561 ttattaacat ggttacaaga tccattcatc aaagatatgc caggaaaacg cgttaacatt
    37621 aatgatggtg tgcccaggtt agatacttta atagacagcc atgtaggtta taaaaggaag
    37681 ttaaatagaa aagataacac atacgatact gtaccactaa tcaaaataaa gtcggtaaaa
    37741 gatacagaaa ttaaaaatgg aaaaaa
//
LOCUS       DAKTLH010000012        35501 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.012, whole genome shotgun sequence.
ACCESSION   DAKTLH010000012 DAKTLH010000000
VERSION     DAKTLH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 35501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35501
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.012"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            194..514
                     /locus_tag="O0394_000516"
     CDS             194..514
                     /locus_tag="O0394_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCY0498620.1"
                     /translation="MPHILNVTVPIPETHVLITKDEYDELIGYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDESFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            511..672
                     /locus_tag="O0394_000517"
     CDS             511..672
                     /locus_tag="O0394_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HCY0498621.1"
                     /translation="MSDTYKSYLLAVLCFTVLAIVLMPLLYFTTAWSIAGFASIGTFI
                     YYKEYFYEE"
     gene            767..1027
                     /locus_tag="O0394_000518"
     CDS             767..1027
                     /locus_tag="O0394_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001794769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HCY0498622.1"
                     /translation="MTKNYKDMTQDELRGLLAEKNAELFDLASEIDEETEFDVLLFSN
                     VGISNGDFTPSSHCVIGNVVDIANLLKRRAVYRDIADVIKMR"
     gene            1067..1327
                     /locus_tag="O0394_000519"
     CDS             1067..1327
                     /locus_tag="O0394_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCY0498623.1"
                     /translation="MYYKTGDVCRKIINVDGFDFQLRVKKRAYSVEMVVLDHEGNSID
                     GLLVSDENDLYTALDILKQSVYEWIENNTDEQDKLMNLVMKW"
     gene            1336..1599
                     /locus_tag="O0394_000520"
     CDS             1336..1599
                     /locus_tag="O0394_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498624.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEVLRGHRKIKKGA"
     gene            1608..3551
                     /locus_tag="O0394_000521"
     CDS             1608..3551
                     /locus_tag="O0394_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCY0498625.1"
                     /translation="MKINKLTISNFAGIKEEKFNFDGKDAKIYGNNATGKTTTATALQ
                     WLLFDKGLDGSTKSFNPVPLNEKNEENYELIPTVFAEFEIDGKITTFKKESHPKYTIN
                     QKTNRKEYSRSRTKKQYINDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLDWKKR
                     RSLLFEIAKPINDEDVIKTNDDFKELNNILGDHEIETKKKILTDKIKQINKDIKDIPI
                     RINQTQQNKQDVPEFDNDRYAIIKQEIEQLENERIDIQNGKEEINLRNQLADKQSELK
                     RIEDNNSASNENKIHALTNELHVENGTVANLKTRLKQNKQQITHEENRRNQLLENHKG
                     LKSDLEKAKNQKFEYLDDNVCSCCGQKLPVEQVDMAREKALQKFNASKSKELETIQTS
                     INHIISEGKKIKLIIEKLEDDNNNLQIKINEAEERSARIQNKINKLKTTHVDVTQTDE
                     YKAVMLEINEINQKRSNIRKTIQDKVSGIDDKISELTQEKSEIEVSRSIEKSNKHLDD
                     VISELRNEEDRLLDEKEKYSHDLYILKEFTTTKVKMLTENINNEFEIAEFKLFNTLVN
                     GELEETCSTTVNGVEYDSGLNNASRINVGLDIINTLSKHFKVTAPIFIDNAESVTELI
                     KTESQQIQLIVNEQDKKLRMETI"
     gene            3553..4473
                     /locus_tag="O0394_000522"
     CDS             3553..4473
                     /locus_tag="O0394_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCY0498626.1"
                     /translation="MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYS
                     PSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQR
                     SYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIG
                     AYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVI
                     NRAAKRYINTSTDSNIFKYAQESEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYT
                     ELENDKPIDVSDFEEIKEPATEKESEEEPF"
     gene            4554..5171
                     /locus_tag="O0394_000523"
     CDS             4554..5171
                     /locus_tag="O0394_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0498627.1"
                     /translation="MKFERVQKHFKYKTRHIAGCLITHEHGDHAKYTKQFVDNGVISY
                     MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKVL
                     YVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSTLANRIMESHFSLEHAIG
                     MLKANDLTRLEEIHLIHLSSQNSNAKYIKSEIQKVTGAPVYFGGL"
     gene            5172..5642
                     /gene="ssb"
                     /locus_tag="O0394_000524"
     CDS             5172..5642
                     /gene="ssb"
                     /locus_tag="O0394_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0498628.1"
                     /translation="MLNRTILVGRLTRDPELRTTQNGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVIADSIQF
                     LEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANGPIELNDDDLPF"
     gene            5672..6565
                     /locus_tag="O0394_000525"
     CDS             5672..6565
                     /locus_tag="O0394_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCY0498629.1"
                     /translation="MTGWISIDRSIQNHWLFKEKRTFSKFEAWIYLLMEANHSKAKVP
                     IGNQIVTVERGQRLTSILTLSDLFNWSRFKVKTFLDLLESDGMLEVKTTSKYTLITIV
                     NYDFYQSEQGRNQHQNDIKPTSKQHQSNINPTSKQHQTNTNNNDNKDNNEKNVNNEKK
                     KTTAFDFFQDNGFGFITSYNLDDLNYYLDSFENDSDEIVTASLKIAKDRNKVTWGYAK
                     SILNTWLNANLKSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPE
                     VDENLEKDREAFIKRLNSKWE"
     gene            6572..6790
                     /locus_tag="O0394_000526"
     CDS             6572..6790
                     /locus_tag="O0394_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498630.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDGRHLVVGLKHTKFNGRRW
                     YLDDYELKTLIDNEQMELGHQTSLFEYI"
     gene            6799..7203
                     /locus_tag="O0394_000527"
     CDS             6799..7203
                     /locus_tag="O0394_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCY0498631.1"
                     /translation="MEIEIKFNEVFNAPMGSPRPRFSTKGRYAHTYMPTKYTEHKKYL
                     QNQMPKLNLENALKIELEFYFTPPKSWSKKKKTQAIGQLKVTKPDIDNLMKTVLDACN
                     NYLWKDDNQITEITSSKRYGIEPKIIIRIEEI"
     gene            7216..7584
                     /locus_tag="O0394_000528"
     CDS             7216..7584
                     /locus_tag="O0394_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498632.1"
                     /translation="MARKARIVTINDKPYRFTKSEMELIESHGITAGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKRKKEAELRRKKPHLFNVPQKH
                     PRGRYACYLMENDIFVKVKK"
     gene            7588..7830
                     /locus_tag="O0394_000529"
     CDS             7588..7830
                     /locus_tag="O0394_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phi PVL orf 51-like protein"
                     /protein_id="HCY0498633.1"
                     /translation="MTDNARKEYLNQFFGSKRYLYQDNERVAHIHVVNGTYYFHGHIV
                     PGWQGVKKTFDTAEELETYIKQQGLEYEEQKQLTLF"
     gene            7839..8210
                     /locus_tag="O0394_000530"
     CDS             7839..8210
                     /locus_tag="O0394_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0498634.1"
                     /translation="MKVNYETGFQIGVMEARLKKMRKQRDEYKKQRDELIGDIAKLRE
                     RNEELENMWRTLKNELFGRYEFYRFRLSELQIESRANKEVAIYRRAEINLSVILCRMD
                     KLDGTNEFYEFLGQMEDDTNE"
     gene            8203..8439
                     /locus_tag="O0394_000531"
     CDS             8203..8439
                     /locus_tag="O0394_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCY0498635.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEIRE
                     AINAQWVEYPEDWASEVLREVRELEYEEEQENEN"
     gene            8429..8671
                     /locus_tag="O0394_000532"
     CDS             8429..8671
                     /locus_tag="O0394_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498636.1"
                     /translation="MKINESLKKLKEKGYKENEDKAIFNLADGTLEIYIDHDEKTIIT
                     EFHDLKVFVSEDLKDKSMESVMYELAGIDEEDKEND"
     gene            8664..9197
                     /locus_tag="O0394_000533"
     CDS             8664..9197
                     /locus_tag="O0394_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HCY0498637.1"
                     /translation="MTNILQVKLLSENARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKIDAGYHGNLGINIKNDNETLESE
                     DMSNFGRSPAGIDGKYARLPVTDKILCMNGSYVINKGDKLAQLVIVPIWTPELKQVEE
                     FESVSERGAKGFGSSGV"
     gene            9234..9479
                     /locus_tag="O0394_000534"
     CDS             9234..9479
                     /locus_tag="O0394_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498638.1"
                     /translation="MSDMLEIFLIGFGVYLFYRIAIIFLKSKKTIHTNIYEMLMLATI
                     FMISTFAYKHQKTHILIAFLVMFFMSKLKQVQGSYEE"
     gene            9476..9682
                     /locus_tag="O0394_000535"
     CDS             9476..9682
                     /locus_tag="O0394_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCY0498639.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     EKQVKRDAVIKVGQLFENIRECGK"
     gene            9679..10065
                     /locus_tag="O0394_000536"
     CDS             9679..10065
                     /locus_tag="O0394_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498640.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGITALLLVVALPIYTVASYQNK
                     EVHQGTITDKYNKRQDKEDKFYIVLDDKQVIENSDLFFKGKFDSADIQARLKVGDKVK
                     VKTIGYRIHFLNLYPVLYEVKKVDKK"
     gene            10062..10211
                     /locus_tag="O0394_000537"
     CDS             10062..10211
                     /locus_tag="O0394_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0498641.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
     gene            10211..10411
                     /locus_tag="O0394_000538"
     CDS             10211..10411
                     /locus_tag="O0394_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCY0498642.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYMNDYLLDEVVKT
                     KGYNGLKEYRIELKRMNNDIKK"
     gene            10439..10855
                     /locus_tag="O0394_000539"
     CDS             10439..10855
                     /locus_tag="O0394_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0498643.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            11087..11386
                     /locus_tag="O0394_000540"
     CDS             11087..11386
                     /locus_tag="O0394_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0498644.1"
                     /translation="MMTKDERIRFYKSKEWQTTRKRVLERDNYECQQCKRDGKLTTYD
                     KSKRKSLDVDHILSLEHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            11516..11860
                     /locus_tag="O0394_000541"
     CDS             11516..11860
                     /locus_tag="O0394_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498645.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDDYL"
     gene            11857..13518
                     /locus_tag="O0394_000542"
     CDS             11857..13518
                     /locus_tag="O0394_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCY0498646.1"
                     /translation="MISNKYVDEYINLWKQGKIILNKERIDLFNYLQKHIYSRDDVYF
                     DEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGL
                     ISAISDFLSTPLHGVKEYHISIVANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYE
                     VSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKK
                     NRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWE
                     KANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWK
                     EILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDV
                     KLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAF
                     EDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKVKPDGNK
                     EYIKKDENRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSIDF"
     gene            13534..14721
                     /locus_tag="O0394_000543"
     CDS             13534..14721
                     /locus_tag="O0394_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCY0498647.1"
                     /translation="MSILEKIFKTRKDITYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DSKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVALMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLITKNYEKANSGENDEKEKDENTLKGGDEDE
                     SGD"
     gene            14705..15442
                     /locus_tag="O0394_000544"
     CDS             14705..15442
                     /locus_tag="O0394_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCY0498648.1"
                     /translation="MKAEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEA
                     IEQGFADSKMFENDNMQIVASNTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKVSANGFSRFLF"
     gene            15466..16611
                     /locus_tag="O0394_000545"
     CDS             15466..16611
                     /locus_tag="O0394_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCY0498649.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQSLSANQRSFFMDINKNVNYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETAFLKGTGKDQPIGLNRQVQKGVSVT
                     EGAYPEKEEQGTLTFANPRATVNELTQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEGTEETL"
     gene            16631..16915
                     /locus_tag="O0394_000546"
     CDS             16631..16915
                     /locus_tag="O0394_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498650.1"
                     /translation="MVKFKVVRAFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLELCESLQKKASSSMVKSEIVDLLNGEDNDD"
     gene            16905..17189
                     /locus_tag="O0394_000547"
     CDS             16905..17189
                     /locus_tag="O0394_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCY0498651.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            17173..17535
                     /locus_tag="O0394_000548"
     CDS             17173..17535
                     /locus_tag="O0394_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HCY0498652.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            17532..17936
                     /locus_tag="O0394_000549"
     CDS             17532..17936
                     /locus_tag="O0394_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCY0498653.1"
                     /translation="MSVKVIGDKALERELENRFGTQAMLKVQDRALIAGAKVIVEEVK
                     KQLKPSKDTGALINEVSFSKPEWINGKRTITVHWRGSKDRYKIVHLIENGHVQKGTGK
                     FIKPKAMGGVNRAIRQGQNKYFETLKRELKKL"
     gene            17933..18337
                     /locus_tag="O0394_000550"
     CDS             17933..18337
                     /locus_tag="O0394_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498654.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDERAYSYIVQIDVFVKFNEKYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSTRVYEGIFYEEE"
     gene            18341..18985
                     /locus_tag="O0394_000551"
     CDS             18341..18985
                     /locus_tag="O0394_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0498655.1"
                     /translation="MVVKQASAPKAYFNITGLGFAKLTKEGAKLEYSDITKTRGLQKI
                     GVETGGDLKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEEYDEYGVYKET
                     QGKQNNYVAIWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLIDNKKSVRKYIFDSANMTNHSGNGEKGEEAFLKKILGEEYTGNVTEDNGETL"
     gene            19012..19251
                     /locus_tag="O0394_000552"
     CDS             19012..19251
                     /locus_tag="O0394_000552"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCY0498656.1"
                     /translation="MKSVNIPDSFKTLKVGNTYDLNVVVEPSNQSKLLKYTTDQTNIV
                     SINSDGQVTAEAQGIATVKAYVGNMSDTITINVEA"
     gene            19301..19651
                     /locus_tag="O0394_000553"
     CDS             19301..19651
                     /locus_tag="O0394_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498657.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFAVKDLKERMHAPDGMNALREQVVFITQG
                     QQTEETRNFIQNMK"
     gene            19896..24425
                     /locus_tag="O0394_000554"
     CDS             19896..24425
                     /locus_tag="O0394_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCY0498658.1"
                     /translation="MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNF
                     KYTEKSTDSYKQRIKELDGTITGYKKNVDDLAKQYDKVSQEQGENSAGAQKLRQEYNK
                     QANELNYLERELQKTSAEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGL
                     KSIGKGMMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGSELKKLQNSFKDVY
                     GNFPADAETVGGVLGEVNTRLGFTGKELENATESFLKFSHITGSDGVQAVQLITRAMG
                     DAGIEASEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQW
                     EKSGVNTEIAFSGLKKAISNWGKAGKNPREEFKKTLAEIEKTPDIASATSLAIEAFGA
                     KAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGAD
                     VWASIESAFAPVMEELIKKLSVAVDWFSNLSDGSKRSIVIFGGIAAAIGPVVFGLGAF
                     ISTIGNAVTVLAPLLAGIAKADGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAV
                     AFTIAYKKSETFRNFVNDAIESVKQTFSNFIQFIQPFIDSVKNVFKQAVSAIVDFAKD
                     IWSQINGFFNENGISIVQALQNICNFIKVIFEFILNFVIKPIMFAIWQVMQFIWPAVK
                     ALIVSTWENIKGVIQGTLNIILGLIKFFSSLFTGDWRGVWDAIIMILKGVVQLIWNLI
                     QLWFVGKILGVVRYFGGLLKGLITGIWDVIKSIFSKSLSAIWNATKSIFGFLFNSVKS
                     IFTNMKNWLSNTWSSIRTNTIGKAQSLFSGVKSKFTSLWNATKDIFSNLRNWMANIWN
                     SIKDNTVGIASRLWSKVRGIFTNMRDGLQSIISKIKSHIGGMVDAIKKGLNKLIEGLN
                     WVGGKLGMDKIPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEII
                     EFPNGKRVITPNTDTTAYLPKGSKVYNGTQTYSMLNGTLPRFSLGTMWKDIKSGASSA
                     FNWTKDQIGKGTKWLGDKVGDVLDFMENPGKLLNYILEAFGIDFNSLTKGMGIAGDIT
                     KAAWSKIKKSATDWIKENLEAMGGGDLVGGILDPDKINYHYGRTAAYTAATGRPFHEG
                     VDFPFVYQEVRTPMGGRLTRMPFMSGGYGNYVKITSGVIDMLFAHLKNFSKSPPSGTM
                     VKPGDVVGLTGNTGFSTGPHLHFEMRRNGRHFDPEPYLRNAKKKGRLSIGGGGATSGS
                     GATYASRVIRQAQSILGGRYKGKWIHDQMMRVAKRESNYQSNAVNNWDINAQRGDPSR
                     GLFQIIGSTFRANAKRGYTNFNNPVHQGISAMQYIVRRYGWDGFKRAGDYAYATGGKV
                     FDGWYNLGEDGHPEWIIPTDPARRNDAMKMLHYAAAEVRGRKASKNKRPSQLSSVNGF
                     DDPSLLLKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIEKRERQ
                     ESTKVKFRKGGIAIQ"
     gene            24422..25906
                     /locus_tag="O0394_000555"
     CDS             24422..25906
                     /locus_tag="O0394_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCY0498659.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGSIYK
                     GRRLESYSFDIPLVVRNDYLSHNGFKTHDDVLNELVKFFNYEEQVKLQFKSKDWYWNA
                     YFEGPIKLHKEFAIPVKFTIKVVLTDPYKYSVTGYKNTAISDQVSVVNSGTADTPLIV
                     EARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITEDI
                     PSNDLGGKVGGDFVISNLGEGYKATNFSDAKGWVGAGTKRGLPKAMTDFQITYKCIVE
                     QKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQNK
                     PIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQRP
                     ASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSVVI
                     NEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQDRYL"
     gene            25922..29707
                     /locus_tag="O0394_000556"
     CDS             25922..29707
                     /locus_tag="O0394_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498660.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPMRYIWGIYEPQSDDQNMNETRLSSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNLIHQKLNDNISNINTIVKDVVDGELEYFERKIHKSDT
                     PPENPVNDTLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTKTLFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDNLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANA
                     YTDNKVKESTDAQRRTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNV
                     TDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNS
                     LNLSREGLDINVNRIGIKGGNNNRYVQIQNDSIELGGIVQRTWKGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRFAFTLSNADNAYSS
                     DGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIEAGYGKFNMLKRRD
                     GNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSAAANLHITSAGTIGRSTSARKYK
                     LSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDRKLSEDTYKLDRYV
                     GLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQLRIKKLEESKNAG"
     gene            29697..29849
                     /locus_tag="O0394_000557"
     CDS             29697..29849
                     /locus_tag="O0394_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498661.1"
                     /translation="MQDNKQGLQANPEYTIHYLSQEIMRLTQENAMLKAYIQENKENQ
                     QCAEEE"
     gene            29896..30183
                     /locus_tag="O0394_000558"
     CDS             29896..30183
                     /locus_tag="O0394_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498662.1"
                     /translation="MAKEIINNTERFILVQIDKEGTERVVYQDFTGSFTTSEMVNHAQ
                     DFKSEENAKKIAETLNLLYQLTNKKQRVKVVKEVVERSDLSPEVTVNTETV"
     gene            30241..30537
                     /locus_tag="O0394_000559"
     CDS             30241..30537
                     /locus_tag="O0394_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCY0498663.1"
                     /translation="MGWFKKHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIKL
                     DKTLDAIQKEREIDEKNKKENDKNIRDMKMWVLGLVGTIFGSLIIALLRMLMGI"
     gene            30687..30863
                     /locus_tag="O0394_000560"
     CDS             30687..30863
                     /locus_tag="O0394_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCY0498664.1"
                     /translation="MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIAL
                     IGLVIKLIELSNKK"
     gene            complement(30916..31023)
                     /locus_tag="O0394_000561"
     CDS             complement(30916..31023)
                     /locus_tag="O0394_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498665.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPNEPVKKTVAILD"
     gene            31075..31329
                     /locus_tag="O0394_000562"
     CDS             31075..31329
                     /locus_tag="O0394_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCY0498666.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            31341..32096
                     /locus_tag="O0394_000563"
     CDS             31341..32096
                     /locus_tag="O0394_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0498667.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYTPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF"
     gene            32287..32778
                     /gene="sak"
                     /locus_tag="O0394_000564"
     CDS             32287..32778
                     /gene="sak"
                     /locus_tag="O0394_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HCY0498668.1"
                     /translation="MLKRGLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVII
                     EKK"
     gene            33428..33763
                     /locus_tag="O0394_000565"
                     /pseudo
     CDS             33428..33763
                     /locus_tag="O0394_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(33858..34307)
                     /locus_tag="O0394_000566"
     CDS             complement(33858..34307)
                     /locus_tag="O0394_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-inhibiting protein CHIPS"
                     /protein_id="HCY0498669.1"
                     /translation="MKKKLATTVLALSFLTAGISTHHHSAKAFTFEPFPTNEEIESNK
                     KMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLKKGTKNSAQFEKMVILTENKGYYTV
                     YLNTPLAEDRKNVELLGKMYKTYFFKKGESKSSYVINGPGKTNEYAY"
     gene            34992..35342
                     /gene="scn"
                     /locus_tag="O0394_000567"
     CDS             34992..35342
                     /gene="scn"
                     /locus_tag="O0394_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HCY0498670.1"
                     /translation="MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNE
                     KLANELKSLLDELNVNELATGSLNTYYKRTIKISGQKAMYALKSKDFKKMSEAKYQLQ
                     KIYNEIDEALKSKY"
BASE COUNT        13485 a         4931 c         7019 g        10066 t
ORIGIN      
        1 aatgatgtgt agagttttgc tttcagcatc tttcatatgt tcttgtactt taataacttc
       61 accattaatt aaatgtaaat ctaaagaagt aacgatttct ttatctaaat attgagtgag
      121 caatctctta tgcatactta tcacctcctt tcactaggag ataacaacat tatacacgaa
      181 aggaaagata gaaatgccac acattttaaa cgtaacagtt ccaatacctg aaacacatgt
      241 acttatcaca aaagatgaat atgatgagct aattggttat tcattagacc ctgtatggaa
      301 catgagtgac ttaaagaaga aattaaaaat tgcatctgat gagactatca aggacagatt
      361 actatttcat cctagatttg aaaaagaact aagagcgcaa ggaattgtgc attacccaga
      421 tgagagtttt aatcgctgga gatttaacgc aagaaagatg aataaattcg tcgatgagca
      481 tttcaatgaa atatataagg agagaataaa atgagcgaca catataaaag ctacctatta
      541 gcagtgctat gtttcacagt cttagcgatt gtgcttatgc cattgctgta cttcactaca
      601 gcgtggtcaa ttgcaggatt cgcaagcatt ggaacattca tatactacaa agaatacttt
      661 tatgaagaat aaaaaaactg ctacttgcgt caacaagtaa cagtgacaaa cgattaacaa
      721 aattaattca ttttcaatat aaaacgaaac aaggaggaag tcaaccatga ctaaaaatta
      781 taaagatatg actcaagacg aattaagggg tttattggct gaaaagaatg cagaattgtt
      841 tgatttagcg agcgaaatcg atgaagaaac tgaatttgac gttttacttt tttcaaatgt
      901 agggattagc aacggagatt ttacaccgtc gtcacattgt gtgattggga atgttgttga
      961 tattgctaat ttattgaaac gtagagctgt ttaccgagat atcgctgatg ttatcaaaat
     1021 gcgttaaaaa ttctagctat agataatgac aaggaggacg agaacaatgt attacaaaac
     1081 gggtgacgta tgtcgaaaaa taattaatgt agatggcttt gattttcaat taagagttaa
     1141 gaaacgagcg tatagcgtcg aaatggttgt tctagaccat gaaggaaatt caattgacgg
     1201 gttactagtt tctgatgaga acgatctata cacagcttta gatattttga aacaaagtgt
     1261 ttatgaatgg attgaaaata acacagatga acaggacaaa ctaatgaact tagtcatgaa
     1321 atggtaggta taagcatgag agatacagaa agaaatatat tgaatatttt taagacgtta
     1381 ttcgacgaat atactttgtc aaaccaacga gcattattgg aaattgaacg taatcatcac
     1441 ggatacttat cgattaattt cttgcactat cacgacagtt acaaaacaaa caataagctt
     1501 gtgcagatac atgaaatcaa tccagacagc catgaacgaa taaaaaattt aattatcgag
     1561 gtactaagag gtcatcggaa gattaaaaaa ggagcatgag gaaggatatg aaaataaata
     1621 aattaacaat atcgaacttt gccggaatca aagaagaaaa atttaatttt gacggtaaag
     1681 atgcaaaaat atacggcaat aatgcgactg gtaagactac aacagcaacc gcattacaat
     1741 ggttgctttt cgataaaggt ttggacggtt caaccaaatc atttaaccct gtacctttaa
     1801 acgaaaaaaa cgaagaaaat tatgagttaa ttccgactgt tttcgcagaa tttgaaatcg
     1861 acggaaaaat tacgactttt aaaaaagagt cacatcctaa atacacaata aatcaaaaga
     1921 cgaatcgcaa ggaatactca cgaagtcgaa cgaagaaaca atatatcaat gatgaatcaa
     1981 taaaagtaaa ggattataaa gctcgtattg atgaacttat tgatgaagat gtattcaagt
     2041 taattacgaa ccctcaagca tttaacttac tagattggaa gaagcgaaga agtttgttgt
     2101 ttgaaattgc taaaccaatc aatgatgagg atgtcattaa aacaaatgat gattttaaag
     2161 aactaaataa tattcttgga gatcatgaaa ttgaaacaaa gaaaaagatt cttacggaca
     2221 agataaaaca gattaacaaa gatatcaaag atattccgat acgtattaac caaacacaac
     2281 aaaataagca ggatgtacca gaattcgata atgatagata cgcaattatc aaacaagaaa
     2341 ttgagcaact tgaaaatgag cgtatagata ttcaaaacgg taaggaagaa attaatttac
     2401 gtaatcaatt agctgataaa caatcagaat tgaaacgcat agaagacaat aacagcgcaa
     2461 gtaatgagaa caaaatccat gctttaacaa atgaattaca cgttgaaaat ggaacggtag
     2521 caaaccttaa aacgagatta aagcaaaaca aacaacaaat cacgcatgaa gaaaatagac
     2581 gtaatcaatt attagaaaat cataaaggat taaaaagtga tttagaaaaa gctaaaaatc
     2641 aaaaatttga atatcttgat gacaatgtat gtagttgttg tggtcaaaag ttaccagttg
     2701 aacaagtgga tatggcaaga gaaaaagcat tgcagaaatt caatgctagc aaatcgaaag
     2761 aattagaaac aatacaaaca tctatcaatc acattatttc agaaggcaag aaaataaagc
     2821 taatcatcga gaagttagag gatgacaata ataatcttca aattaaaatc aacgaagcag
     2881 aagagcgttc agcaagaata caaaacaaaa ttaataagtt gaaaacaact cacgttgacg
     2941 ttacgcaaac tgacgaatac aaagcagtaa tgttagagat aaatgagatt aatcaaaaac
     3001 gctctaacat caggaaaact attcaagata aagtttcagg aatagatgac aaaataagcg
     3061 aacttactca agaaaaatca gaaattgaag tgtcaagatc aatcgaaaaa tcaaataaac
     3121 atctagatga tgttatttct gaattaagaa atgaagaaga cagattattg gatgaaaaag
     3181 aaaagtattc acatgacctt tatatcttaa aagaatttac aacaacaaaa gtcaaaatgc
     3241 ttactgaaaa catcaataat gaatttgaaa ttgctgaatt taagctattc aataccttag
     3301 ttaacggcga attagaagaa acatgttcca caacggttaa cggcgtcgaa tacgacagcg
     3361 gtttaaataa cgcctcaaga attaatgttg gcttagatat catcaataca ctgtcaaaac
     3421 attttaaagt tacagcgcca atatttattg ataatgctga atcagtaaca gagcttatca
     3481 aaacagaatc acaacaaatt caattgatag taaatgaaca agataaaaaa ttaagaatgg
     3541 agactatata aaatgactga aaataataaa ttacaaacta ttgaacaaca attagtacaa
     3601 gaaaagaacg tatctgacaa cgtattaaac aaagtgagag ttttagagtc acaaggcaat
     3661 ttggaattgc caaatgatta ttcaccaagt aatgccatga aacaagcatg gttacaaatc
     3721 agccaagata acaaattaat gagttgtaac gatacaagca aagcaaatgc cttattagac
     3781 atggtaacgc aaggtttaaa tccagctaaa aatcaatgct actttattcc ttacggcaac
     3841 aaaatgcagt tacaacgtag ctatcacggt aatgtaatga tgttaaaacg tgatgcaggt
     3901 gctcaagatg ttgttgctca agtgatttat aaaggcgata cattcaagca agaaatggga
     3961 gaaacaggac gtatcaaagc gattaaacac gaacaagact tctttaacat cgacaaagaa
     4021 aacattatcg gtgcgtactg cacaatcgta tttaatgatg gacgagataa ctatattgaa
     4081 gtcatgacta ttgaacaaat taaacaagca tggatgcagt catcaatgat taaagatgaa
     4141 aaagcattac aaaattctaa aacacataat aatttcaaag aagaaatggc taaaaaaaca
     4201 gttatcaata gagctgctaa acgttatatc aacacatcaa cagatagcaa tattttcaaa
     4261 tacgcacaag aatccgaaca acgtcaacgc aaagaagtgt tggacgcaga agttgaagaa
     4321 aatgcaaatc aagaacaatt ggactttgaa caaccagttc ttgaagaagc acaatacaca
     4381 gaattagaaa atgataagcc tattgatgta tctgactttg aagaaataaa agaacctgca
     4441 acagaaaaag aaagcgaaga agagccattt taattgaaac aatagcaact ggttcaagtg
     4501 gtaactgcta cgtcttaaat gatggacgta ctacgttact acttgaggca ggtataaaat
     4561 ttgaacgtgt tcaaaagcat ttcaaatata aaacaagaca tatagcaggg tgtcttatca
     4621 cacacgaaca tggtgatcat gcaaagtata caaagcagtt tgtcgacaat ggtgtaatca
     4681 gctatatgac tgctggaaca caacaagcta tgaattttga aagtcatcgc ttatgcacga
     4741 ttaaggcaaa gcaagagctg cgaataggta catggtcaat tctaccgttt gacatcgaac
     4801 atgatgctaa cgagcctgtg gctttcttat tacaaagcac actaggttat aaggttctgt
     4861 atgttactga tacaaagtat ttgaaataca aatttaatgg cattacgcac atgatgctag
     4921 aagttaatta tatctatgaa caaatgcaag aaaacataaa aaacggcagt gtgcacagca
     4981 cattagcaaa cagaattatg gagtctcatt ttagcttaga acatgctatc ggaatgttaa
     5041 aagcaaatga tttaactaga ctcgaagaaa tacatttaat tcatttaagt agtcaaaatt
     5101 caaatgcaaa atacattaaa agtgaaatac aaaaagtgac gggcgcgccc gtttattttg
     5161 gaggtttata aatgctaaac agaacaatat tagttggtcg tttaactaga gacccagaat
     5221 taagaaccac tcaaaatggt gtaaatgtag catcattcac attagcagtt aaccgtacat
     5281 ttacaaatgc acaaggcgag cgcgaggcag actttattaa tatcatcgta tttaaaaaac
     5341 aagcagagaa cgttaataaa tacctatcta aaggatcgtt ggcgggcgta gatggtaggt
     5401 tacaaacgcg gaactatgaa aataaggaag gtcaacgtgt atacgttacg gaagttattg
     5461 ctgatagtat tcaattttta gaaccgaaaa actcaaatga cactcaacaa gatttatatc
     5521 aacaacaagt acaacaaaca cgtggacaat cgcaatattc aaataacaaa ccagtaaaag
     5581 ataatccgtt tgcgaatgca aatggtccga ttgaactaaa tgatgatgat ttaccattct
     5641 aatttaaccg gtttgaaagt gaggtgtgta tatgactggt tggataagta ttgatcgctc
     5701 aattcaaaat cattggctat ttaaagaaaa gagaacattt tcaaagtttg aagcatggat
     5761 atatttactc atggaagcga atcattcaaa ggcaaaagtg cctataggaa accaaattgt
     5821 aaccgttgaa agaggacaaa gattaacatc tattttgacc ttgtctgacc tttttaactg
     5881 gtcacgattt aaagtgaaaa ccttccttga cttactcgag agtgatggaa tgttagaagt
     5941 caaaacaaca tcaaaatata cccttataac cattgtcaat tatgactttt atcaaagtga
     6001 gcagggcagg aaccaacatc aaaacgacat caaaccaaca tcaaaacaac atcagtcaaa
     6061 catcaaccca acatcaaaac aacatcaaac caacacaaac aataatgata ataaagataa
     6121 taatgaaaag aatgtgaata atgagaagaa gaagacaacc gccttcgact tcttccaaga
     6181 taacggattc ggtttcataa cttcttacaa tttagacgat ttaaattatt atcttgattc
     6241 atttgaaaat gattcagatg aaatagttac cgcatcactt aaaatcgcta aagacagaaa
     6301 caaagttact tggggatatg ctaaaagcat tttgaataca tggcttaatg caaacttgaa
     6361 atctattgaa caagtacgtg catttgaaaa gcaacaactt gaaagcaaaa aacaaaatta
     6421 taaacctttc gttaaacaat caaaagaaaa aacacccaaa tggctcacag acagcacgag
     6481 agaaacgaaa acgccggaag tagatgaaaa ccttgagaaa gacagagaag cttttattaa
     6541 gcgtctaaat agcaaatggg agtgattgaa aatggatgca tttgataaat actatctatt
     6601 tgatcatgac ggcaacaaaa tgttttcagt tacaccacat tttaaagatg gacggcattt
     6661 agttgttgga ttaaaacaca caaaattcaa tggtcgacgt tggtacttag atgattatga
     6721 attaaaaaca cttattgata atgaacaaat ggagttagga caccaaacaa gcttatttga
     6781 atatatatga gggattacat ggagatagaa attaaattta atgaagtgtt taatgcgccg
     6841 atgggctcgc ctcgaccgcg ttttagcaca aaaggtagat atgcacacac atatatgcct
     6901 acaaaatata cagaacataa aaaatattta caaaatcaaa tgccaaagct aaatctagaa
     6961 aatgcattaa aaattgaatt agagttttac tttactccac ctaaatcatg gtcgaagaaa
     7021 aagaaaactc aagcgattgg acaattaaaa gtaactaaac cagatatcga taatttgatg
     7081 aagacagttc tagacgcttg taacaattat ttgtggaaag acgataacca aattacagaa
     7141 ataactagct caaagcgtta tggaattgag cccaaaataa tcatacgaat agaagaaata
     7201 taagaggtgg ataaaatggc gagaaaagca aggattgtaa caataaatga taaaccatat
     7261 aggtttacca aatctgaaat ggaattaata gaaagtcacg gtataaccgc tggaatggtt
     7321 tctaagagag taaaagacgg ttgggaacta catgaagcaa tggacgcacc agaaggtacg
     7381 cgtttaagcg agtacagaga aaagaaaaca atagaaagac tggaacaagc tagactcgaa
     7441 cgcaaattgg aaagaaagcg aaagaaagag gctgagctaa gaagaaagaa gccacatttg
     7501 tttaatgtac cacagaaaca tccaagagga cgttatgcgt gctacctgat ggaaaacgac
     7561 atattcgtga aagttaagaa gtagatcatg acagataacg cacgcaaaga atacctaaat
     7621 caattctttg gatctaagag atatctgtat caggataacg aacgagtggc acatattcat
     7681 gtagtaaacg gcacttatta ctttcatggg catatcgtac caggttggca aggcgtgaaa
     7741 aagacatttg atacagctga agagcttgaa acatatataa agcaacaggg tttggaatat
     7801 gaggaacaga agcaactaac tttattttag aggaggttat gaaagtgaac tatgaaacag
     7861 gattccaaat aggcgtaatg gaagctaggt tgaagaagat gagaaaacaa cgtgatgagt
     7921 acaagaagca acgtgacgag cttattgggg atatagctaa gttaagagag cgtaacgaag
     7981 agctggagaa catgtggcgc acgcttaaaa atgaattgtt tggaagatac gaattttacc
     8041 gttttagact tagcgaacta cagattgaga gcagagcgaa caaggaagta gctatatata
     8101 gaagagctga aatcaactta agtgttatat tgtgccgaat ggacaaacta gacggaacaa
     8161 atgagttcta cgaattttta gggcaaatgg aggatgacac aaatgaataa ccgcgaacaa
     8221 atcgaacaat cagttatcag tgctagcgcg tataacggca atgacacaga gggattgcta
     8281 aaagagattg aagacgtgta taagaaagcg caagcgtttg atgaaattcg cgaagctatt
     8341 aatgcgcaat gggttgagta tccagaagac tgggcgtcag aggttttgag agaagtaaga
     8401 gagcttgaat atgaggagga acaggaaaat gaaaattaat gaatctttga agaaattaaa
     8461 agaaaaagga tacaaagaaa atgaagataa agctattttt aatttagctg atggtacgct
     8521 agaaatttac atcgaccatg acgaaaaaac aataattaca gaattccatg atttaaaggt
     8581 attcgtgtca gaagatttaa aagataaaag tatggagagc gttatgtatg aattggctgg
     8641 aattgatgag gaggacaagg aaaatgacta acatcctaca agtaaagcta ttatcagaaa
     8701 acgctagaat gccagaacga aatcataaga cggatgcagg ttatgacata ttctcagctg
     8761 aaactgtcgt acttgagcca caagaaaagg cagtgatcaa aacagatgta gctgtaagca
     8821 taccagaggg ctatgtcgga ctattaacta gccgtagtgg tgtaagtagt aaaacgcatt
     8881 tagtgattga aacaggcaag atagacgcgg gatatcacgg caatttaggg attaatatca
     8941 agaatgataa tgaaacgtta gagagtgagg atatgagtaa ctttggtcgg agtcctgctg
     9001 ggatagatgg aaagtatgcc cgactacctg taacagataa aattttatgt atgaatggta
     9061 gttatgtcat aaacaaaggc gacaaactag ctcaattggt tattgtgcct atatggacac
     9121 ctgaactaaa gcaagtggag gaattcgaga gtgtttcaga acgtggagca aaaggcttcg
     9181 gaagtagcgg agtgtaaaga catattagat cgagtcaagg aggttttggg gaagtgagcg
     9241 acatgttaga aatattttta atagggtttg gcgtttatct cttttatcgc atagcaatta
     9301 tttttcttaa gagtaaaaag actatacaca caaacatata tgaaatgtta atgcttgcta
     9361 ctatctttat gatatctaca tttgcttata aacatcaaaa gacgcatatc ttaatagcat
     9421 ttttagtaat gttttttatg agtaagctca aacaagttca agggagctat gaggaatgac
     9481 acaatactta gtcacaacat tcaaagattc aacaggacgt aaacatacac acataactaa
     9541 agctaagagt aatcaaaggt ttacagttgt tgaggcagag agtaaagaag aagcgaaaga
     9601 gaagtacgag aaacaagtta aaagggatgc agttattaaa gtgggtcagt tgtttgaaaa
     9661 tataagggag tgtgggaaat gattaaaaaa cttaaaaata tggatgggtt cgacatcttt
     9721 attgttggaa tactgtcatt attcggtata accgcattgc tacttgttgt cgcattgcct
     9781 atctatacag tggctagtta ccaaaacaaa gaagtacatc aagggacaat tacagataaa
     9841 tataacaaaa gacaagataa agaggacaaa ttctatattg tattagatga taaacaagtc
     9901 atcgaaaact cagacttatt cttcaaagga aagtttgata gcgcagacat acaagctagg
     9961 ttaaaagtag gtgataaagt aaaagttaag acgattggat atagaataca ctttttaaat
    10021 ttatatccgg tcttatacga agtaaagaag gtagataaaa aatgattaag caaatactaa
    10081 gattattatt cttactagcg atgtatgagt taggtaagta tgtaactgag caagtatata
    10141 ttatgatgac ggctaatgat gatgtagagg cgccgagtga ttacgtcttt cgagcggagg
    10201 taagtgagtg atgtggatta ctatgactat tgtatttgct atattgctat tagtttgtat
    10261 cagtattaat agtgatcgtg caagggagat acaagcgctc agatatatga atgattatct
    10321 acttgatgaa gtagttaaaa ctaaaggata caacgggtta aaagaataca ggattgaatt
    10381 aaagcgaatg aataacgata ttaaaaagta atttatatta tcggaggtat tacattgaat
    10441 gataaagatt gagaaacacg atatcaaaaa gcttgaagaa tacattcagc acatcgataa
    10501 ctatcgaaga gagttgaaga tgcgagaata tgaattactt gaaagtcatg aaccagataa
    10561 tgcgggagct ggcaaaagta atttgccggg taacccgatt gaacgatgtg caataaagaa
    10621 gtttagtgat aacaggtaca atacattaag aaatatagtt aacggtgtag atagattgat
    10681 agatgaaagt gatgaggata cgcttgagtt attaaggttt agatattggg attgtcctat
    10741 tggttgttat gaatgggaag atatagcaca ttactttggt acaagtaaga caagtatatt
    10801 acgtagaagg aatgcactga tcgataagtt agcaaagtat attggttatg tgtagcggac
    10861 ttttacccta tgtaagtccg cattaaaaca gtttattatg ttagtatcag attaatattt
    10921 aaagttatta aatgctaata cgacgcatga acaagaggcg catcactatg tgatgtgtct
    10981 ttttatttat gaggtatgaa catgttcaaa ctaatagtaa atacattact acacatcaag
    11041 tatagatgcg tcttgatact acttaagtta tataaggtga aacattatga tgactaaaga
    11101 cgaacgcata cgattctata agtctaaaga atggcaaaca acaagaaaaa gagtgctaga
    11161 aagagataat tatgaatgtc aacaatgtaa gagagacggc aagttaacga catatgacaa
    11221 aagcaagcgt aagtcgttgg atgtagatca tatattatcg ctagaacatc atccggagtt
    11281 tgctcatgac ttaaacaatt tagaaacact gtgtattaaa tgtcacaaca aaaaagaaaa
    11341 gagatttata aaaaaagaaa ataaatggaa agacgaaaaa tggtaaatac ccccgggtca
    11401 aaaaaatcaa aagcgatcaa aatacttggg gaacggggag gggctcgact tcgcgataat
    11461 tttaaaaatc catgtatacc ccccctctta taaccatttt aaggcaggtg atgaaatgga
    11521 gattatagtt gatgaaaact tagtgcttaa agaaaaagaa aggctgcaag tattatataa
    11581 agacatacct agcaataaat taaaagtagt tgatggttta attattcaag cagcaaggct
    11641 acgtgtaatg cttgattaca tgtgggaaga cataaaagaa aaaggtgact atgatttatt
    11701 tactcaatct gaaaaggcgc caccatatga aagggaaaga ccagtagcca aactatttaa
    11761 tgctagagat gctgcatatc aaaaaataat caaacaatta tcggatttat tgcccgaaga
    11821 gaaagaagac acagaaacgc catctgatga ttacctatga ttagtaataa atacgttgat
    11881 gaatatataa atttgtggaa acaaggaaag ataattttaa ataaagaaag aattgatctc
    11941 tttaattatc tacaaaaaca tatatattca cgagatgatg tatattttga tgaacagaaa
    12001 atcgaggatt gtatcaaatt tattgaaaaa tggtattttc caacattacc atttcaaagg
    12061 tttatcatag ctaatatatt tcttatagat aaaaatacag atgaagcttt ctttacagaa
    12121 tttgctattt tcatgggacg tggaggcggg aaaaacggtc taataagtgc tattagtgat
    12181 tttctttcta cgcccttaca cggagttaaa gaatatcaca tctccattgt tgctaatagt
    12241 gaagatcaag caaaaacatc gtttgatgaa atcagaaccg ttttaatgga taacaaacga
    12301 aataagacgg gtaaaacgcc aaaagctcct tatgaagtta gtaaagcaaa aataataaac
    12361 cgtgcaacta aatcggttat tcgatataac acatcaaaca caaaaaccaa agacggtgga
    12421 cgtgaggggt gtgttatttt tgatgaaatt cattatttct ttggtcctga aatggtaaac
    12481 gtcaaacgtg gtggattagg taaaaagaaa aatagaagaa cgttttatat aagtactgat
    12541 ggttttgtta gagagggtta tatcgatgca atgaagcaca aaattgcaag tgtattaagt
    12601 ggcaaggtta aaaatagtag attgtttgct ttttattgta agttagacga tccaaaagaa
    12661 gttgatgaca gacagacgtg ggaaaaggcg aacccaatgt tacataaacc gttatcagaa
    12721 tacgctaaaa cactgctaag tacgattgaa gaagaatata acgatttacc attcaaccgt
    12781 tcaaataagc ccgaattcat gactaagcga atgaatttgc ctgaagttga ccttgaaaaa
    12841 gtaatagcac catggaaaga aatactagcg actaatagag agataccaaa tttagataat
    12901 caaatgtgta ttggtggttt agactttgca aacattcgag attttgcaag tgtagggcta
    12961 ttattccgaa aaaacgatga ttacatttgg ttaggacatt cgtttgtaag acaagggttt
    13021 ttggatgatg tcaaattaga acctcctatt aaagaatggg aaaaaatggg attattgacc
    13081 attgtcgatg atgatgtcat tgaaattgaa tatatagttg attggttttt aaaggctaga
    13141 gaaaaatatg ggcttgaaaa agtcatagct gataattata gaactgatat tgtaagacgt
    13201 gcgtttgagg atgctggcat aaaacttgaa gtacttagaa atccaaaagc aatacatgga
    13261 ttacttgcac cacgtatcga tacaatgttt gcgaaacata acgtaatata tggagacaat
    13321 cctttgatgc gttggtttac taataatgtt gcagtaaagg ttaaacccga tggtaataaa
    13381 gaatatatta aaaaagatga aaatagaaga aaaaccgatg ggttcatggc ttttgttcac
    13441 gcattatata gagcagacga tatagtagac aaagacatgt ctaaagcgct tgatgcatta
    13501 atgagtatag atttctaata gaggaggtga gacatgagta ttctagaaaa gatatttaaa
    13561 actaggaaag atataacata tatgcttgat ttagatatga tagaagatct atcacaacaa
    13621 gcgtatgtga aacgtttagc gattgatagt tgtattgaat ttgttgcgcg agctgtcgct
    13681 caaagtcatt ttaaagtatt ggaaggtaat agaattcaaa agaatgatgt ttactacaag
    13741 ttaaatataa aaccaaatac tgacttatca agcgatagtt tttggcaaca agttatatat
    13801 aaactaattt atgataacga ggttttaatc gtagtaagtg acagcaaaga attacttatc
    13861 gcagatagct tttacagaga agagtacgct ttgtatgatg atatattcaa agatgtaacg
    13921 gttaaagatt atacttatca acgtactttc acaatgcaag aggtcatata tttaaagtac
    13981 aacaacaata aagtgacaca ctttgtagaa agtctattcg aagattacgg gaaaatattc
    14041 ggaagaatga taggtgcaca attaaaaaac tatcaaataa gagggatttt gaaatctgcc
    14101 tctagcgcat atgacgaaaa gaatatagaa aaattacaag cgttcacaaa taaattattc
    14161 aatactttta ataaaaatca actagcaatc gcgcctttga tagaaggttt tgattatgag
    14221 gaattatcta atggtggtaa gaatagtaac atgccttttt ctgaattgag tgagctaatg
    14281 agagatgcaa taaaaaatgt tgcgttgatg attggtatac ctccaggttt gatttacgga
    14341 gaaacagctg atttggaaaa gaacacgctt gtatttgaga agttctgttt aacaccttta
    14401 ttaaaaaaga ttcagaacga attaaacgcg aaactcataa cacaaagcat gtatttgaaa
    14461 gatacaagaa tagaaattgt cggtgtgaat aaaaaagacc cacttcaata tgctgaagca
    14521 attgacaaac ttgtaagttc tggttcattt acaaggaatg aggtgcggat tatgttaggt
    14581 gaagaaccat cagacaatcc tgaattagac gaatacctga ttactaaaaa ctacgaaaaa
    14641 gctaacagtg gtgaaaatga tgaaaaagaa aaagatgaaa acactttgaa aggtggtgat
    14701 gaagatgaaa gcggagatta aaggcgtcat cgtttccaac gaagataaat gggtttacga
    14761 aatgcttggt atggattcga cttgtcctaa agatgtttta acacaactag aatttagtga
    14821 tgaagatgtt gatattataa ttaactcaaa tggtggtaac ctagtagctg gtagtgaaat
    14881 atatacacat ttaagagctc ataaaggcaa agtgaatgtt cgcatcacag caatagcagc
    14941 aagtgcggca tcgcttatcg caatggctgg tgaccacatc gaaatgagtc cggttgctag
    15001 aatgatgatt cacaatcctt caagtattgc gcaaggagaa gcgaaagatc taaatcatgc
    15061 tgcagaaaca ttagaacatg ttggtcaaat aatggctgag gcatatgcgg ttagagctgg
    15121 taaaaacaaa caagaacttg tagaaatgat ggctagggaa acgtggctaa atgctgatga
    15181 agccattgaa caaggttttg cggatagtaa aatgtttgaa aacgacaata tgcaaattgt
    15241 agcaagcaat acacaagtgt tatcgaaaga tgtattaaat cgtgtaacag ctttggtaag
    15301 taaaacgcca gaggttaaca ttgatattga cgcaatagca aataaagtaa ttgaaaaaat
    15361 aaatatgaaa gaaaaggaat cagaaatcga tgttgcagat agtaaagtat cagcaaatgg
    15421 attttcaaga ttcctttttt aatacaaaaa ataggaggtc ataaaatgac tataaattta
    15481 tcggaaacat tcgcaaatgc gaaaaacgaa tttattaatg cagtaaacaa cggtgaaccg
    15541 caagaaagac aaaatgaatt gtacggtgac atgattaacc aactatttga agaaactaaa
    15601 ttacaagcaa aagcagaagc tgaaagagtt tctagtttac ctaaatcagc acaatctttg
    15661 agtgcaaacc aaagaagttt cttcatggat atcaataaaa acgttaacta taaagaagaa
    15721 aaacttttgc cagaagaaac aattgataga atttttgaag atttgacgac gaatcatccg
    15781 ttattagctg atttaggtat taaaaacgct ggtttgcgtt tgaagttctt aaaatctgaa
    15841 acttctggcg tagccgtttg gggtaaaatc tatggtgaaa ttaaaggtca attagatgct
    15901 gcgttcagtg aagaaacagc aattcaaaat aaattgacag cgtttgttgt tttaccaaaa
    15961 gatttaaatg attttggtcc tgcgtggatt gaaagatttg ttcgtgttca aatcgaagaa
    16021 gcatttgcag tggcgcttga aactgcgttc ttaaaaggta ctggtaaaga ccaaccaatc
    16081 ggcttaaacc gtcaagtaca aaaaggtgta tcggtaactg agggtgctta tccagagaaa
    16141 gaagaacaag gtacgcttac atttgctaat ccgcgcgcta cagttaatga attgacgcaa
    16201 gtgtttaaat accactcaac taacgagaaa ggtaaatcag tagcggttaa aggtaatgta
    16261 acaatggttg ttaatccgtc cgatgctttt gaggttcaag cacagtatac acatttaaat
    16321 gcaaatggcg tatatgttac tgctttacca tttaatttga atgttatcga gtctacagtc
    16381 caagaagcag gtaaggtttt aacgtacgtt aaaggtttat atgatggtta tttagctggt
    16441 ggtattaatg ttcagaaatt taaagaaaca cttgcgttag atgatatgga tttatacact
    16501 gcaaaacaat ttgcttacgg caaagcgaaa gataataaag ttgctgctgt ttggaaatta
    16561 gatttaaaag gacataagcc agctttagaa ggtaccgaag aaacactata aaattttatg
    16621 aggtgataaa atggtgaaat ttaaagttgt tagagctttt aaagacatag agcacaatca
    16681 acacaagtac aaagtagggg agttgtatcc agctgaaggg tataacaatc ctcgtgttga
    16741 attgttgaca aatcaaatca aaaataagta cgacaaagtt tatatcgtac ctttagataa
    16801 gctgacaaaa caagaattat tagaactatg cgaatcatta caaaaaaaag cgtctagttc
    16861 aatggttaaa agtgaaatcg tcgacttatt gaatggtgaa gacaatgacg attgatgatt
    16921 tgcttgtcaa atttaaatca cttgaaaaga ttgaccataa ttcagaggat gagtacttaa
    16981 agcagttgtt aaaaatgtcg tacgagcgta taaaaaatca gtgcggagtt tttgaattag
    17041 agaatttaat aggtcaagaa ttgatactta tacgcgctag atatgcttat caagatttat
    17101 tagaacactt caacgataat tacagacctg aaataataga tttttcgtta tctctaatgg
    17161 aggtatcaga agatgaagaa agtgtttaaa aaacctagaa ttacaactaa acgtttaaat
    17221 actcgtgttc atttttataa gtatactgaa aataatggtc cagaagctgg agaaaaagaa
    17281 gaaaaattat tatatagctg ttgggcgagt attgatggtg tctggttacg tgaattagaa
    17341 caagctatct caaacggaac ccaaaatgac attaaattgt atattcgtga tccgcaaggt
    17401 gattatttac ccagtgaaga acattatctt gaaattgaat caagatattt caaaaatcgt
    17461 ttgaatataa agcaagtatc accagatttg gataataaag actttattat gattcgtgga
    17521 ggatatagtt catgagtgtg aaagtgatag gtgataaagc attagaaaga gaattagaaa
    17581 atcgtttcgg aactcaagcg atgttaaaag ttcaagatag ggcgctaatt gctggtgcta
    17641 aagtaattgt tgaagaagta aaaaaacaac taaagccatc aaaagatacg ggcgcattaa
    17701 ttaatgaggt aagttttagt aaacctgaat ggataaacgg aaaacgtaca attactgttc
    17761 attggcgagg ttctaaagac cgttataaaa tcgtacattt aattgaaaat ggacacgttc
    17821 aaaaagggac aggtaaattt atcaaaccta aagctatggg tggcgttaat agagcaataa
    17881 gacaagggca aaataagtat tttgagacgc taaaaaggga gttaaaaaaa ttgtgattga
    17941 tattttgtac aaagttcatg aagtgattag tcaagacaga attattagag agcacgtaaa
    18001 tatcaataat attaagttca ataaataccc aaacgtaaaa gatacggatg tacctttcat
    18061 agttattgac gatatagacg acccaatacc tacaacttat actgacggag atgagcgcgc
    18121 atatagttac attgtacaaa tagatgtatt tgtgaagttt aacgaaaagt ataacgcgag
    18181 aatcataaga aataagatat ctaatcgtat tcaaaagtta ttatggtctg aactgaaaat
    18241 gggaaatgta tccaacggaa aacctgaata tatagaagaa tttaaaacat atagaagcac
    18301 tcgtgtctac gagggcattt tttacgagga ggaataataa atggtagtaa aacaagcaag
    18361 tgcgccgaaa gcgtatttta acattactgg tttaggtttc gctaaattaa caaaagaagg
    18421 cgcaaagcta gaatatagcg atattacaaa aactagagga ttacaaaaaa tcggtgttga
    18481 aactggagga gacttgaaaa cagcatatgc tgatggtggt ccaatcgaat cagggaacac
    18541 agacggagaa ggtaaaattt cgttacaaat gcatgctttc cctaaagaga ttcgcaaaat
    18601 agtattcaat gaagagtatg atgaatacgg tgtttataaa gaaactcaag gtaaacaaaa
    18661 caactatgta gcaatttggt tcagacaaga gcgtcgagac ggcacattta gaacggtttt
    18721 attacctaaa gtcatgttta caaatcctaa aatcgatggc gaaacggctg agaaagattg
    18781 ggatttctca agcgaagagg ttgaaggtga ggcacttttc cctttaattg ataataaaaa
    18841 gtcagtacgt aaatatatct ttgattcagc aaacatgaca aatcatagtg gaaatggtga
    18901 aaaaggcgaa gaggctttct taaagaaaat tttaggcgaa gaatatactg gaaacgtgac
    18961 agaggataac ggcgaaactt tgtaacgcaa ccggcttcat cggaaactga gataaagtcg
    19021 gttaatatac cagatagttt taaaacacta aaagttggca acacatatga tttaaatgtt
    19081 gtagtagagc catctaatca aagtaagtta ttgaaataca caacagatca aacgaatatt
    19141 gtatcaatca atagtgatgg tcaagtcact gcggaagcac aaggtattgc cacggttaaa
    19201 gcatatgttg gtaatatgag tgacactata acaataaatg tagaagcata agaggggaca
    19261 acccctctat tttatttgaa aataaggaga gtattataaa atggcaaaat taaaacgtaa
    19321 cattattcaa ttagtagaag atccaaaagc aaatgaaatt aaattacaaa cgtacttaac
    19381 accacacttc atttcatttg aaattgtata cgaagcaatg gatttaatcg atgatattga
    19441 ggacgaaaat agcacgatga agccaagaga aatcgctgac agattgatgg atatggttgt
    19501 aaaaatttac gataaccaat tcgcagttaa agacctaaaa gaacgtatgc atgcacctga
    19561 tggaatgaat gcacttcgtg aacaagtggt tttcattacg caaggtcaac aaactgagga
    19621 aactagaaat tttatccaga acatgaaata aagcctgaag atttaacata taaagcaatg
    19681 ttgaaaaata tggatactct catgatggac ttaattgaaa atggtaaaga cgctaacgaa
    19741 gttttaaaaa tgccatttca ttatgtgctt tccatatatc aaaataaaaa caatgacatt
    19801 tctgaagaaa aagcagaggc tttaattgat gcattttaac cttaaccgtt tggttagggt
    19861 tatttttttg aactttttta gaaaggaggt aaaaaatggg agaaagaata aaaggtttat
    19921 ctataggttt ggatttagat gcagcaaatt taaatagatc atttgcagaa atcaaacgaa
    19981 actttaaaac tttaaattct gacttaaaat taacaggcaa caacttcaaa tataccgaaa
    20041 aatcaactga tagttacaaa caaaggatta aagaacttga tggaactatc acaggttata
    20101 agaaaaacgt tgatgattta gctaagcaat atgacaaggt atctcaagaa cagggcgaaa
    20161 acagtgcagg agctcaaaag ttacgacaag aatataacaa acaagcaaat gaactgaatt
    20221 atttagaaag agaattacaa aaaacatcag ccgaatttga agagttcaaa aaagctcaag
    20281 ttgaagctca aagaatggca gaaagtggct ggggaaaaac cagtaaagtt tttgaaagta
    20341 tgggacctaa attaacaaaa atgggtgatg gtttaaaatc cattggtaaa ggcatgatga
    20401 ttggtgtaac tgcacctgtt ttaggtatcg cagcagcatc aggaaaagct tttgcagaag
    20461 ttgataaagg tttagatact gttactcaag caacaggcgc aacaggcagt gaattaaaaa
    20521 aattgcagaa ctcatttaaa gatgtttatg gcaattttcc agcagatgct gaaactgttg
    20581 gtggagtttt aggagaagtt aatacaagat taggttttac aggtaaagaa cttgaaaatg
    20641 ccacagagtc attcttgaaa ttcagtcata taacaggttc tgacggtgtg caagccgtac
    20701 agttaattac ccgtgcaatg ggcgatgcag gtatcgaagc aagtgaatat caaagtgttt
    20761 tggatatggt agcaaaagcg gcgcaagcta gtgggataag tgttgacaca ttagctgata
    20821 gtattactaa atacggcgct ccaatgagag ctatgggctt tgagatgaaa gaatcaattg
    20881 ctttattctc tcaatgggaa aagtcaggcg ttaatactga aatagcattc agtggtttga
    20941 aaaaagctat atcaaattgg ggtaaagctg gtaaaaaccc aagagaagaa tttaagaaga
    21001 cattagcaga aattgaaaag acgccggata tagctagcgc aactagttta gcgattgaag
    21061 catttggtgc aaaggcaggt cctgatttag cagacgctat taaaggtggt cgctttagtt
    21121 atcaagaatt tttaaaaact attgaagatt cccaaggcac agtaaaccaa acatttaaag
    21181 attctgaaag tggctccgaa agatttaaag tagcaatgaa taaattaaaa ttagtaggtg
    21241 ctgatgtatg ggcttctatt gaaagtgcgt ttgctccagt catggaagaa ttaataaaaa
    21301 agctatcagt cgctgtcgat tggttttcga atttaagtga tggttctaaa agatcaattg
    21361 ttattttcgg tggtattgct gctgcaattg gtcctgtagt ttttgggtta ggtgcattta
    21421 taagcacaat tggtaatgca gtaactgtat tagccccact attagctggt attgcaaagg
    21481 ctgatggatt aattagtttt ttatcgacta aagtacctat attaggaact gtcttcacag
    21541 ctttaactgg tccaattggc attgtattag gtgttttagc tggcctagca gtcgcattta
    21601 caattgctta taagaaatct gaaacattta gaaattttgt taatgatgca attgaaagtg
    21661 ttaaacaaac atttagtaat tttattcaat ttattcaacc tttcattgat tccgttaaaa
    21721 acgtctttaa acaagcggtt tcagcaatcg ttgatttcgc taaagatatt tggagtcaaa
    21781 ttaatggatt ctttaatgaa aacggaattt ctattgttca agcgcttcaa aatatatgca
    21841 attttatcaa agttatattt gaatttatct taaattttgt aattaaacca ataatgtttg
    21901 caatctggca agtgatgcaa tttatttggc cggcggttaa agccttgatt gtcagtactt
    21961 gggagaatat aaaaggtgtg atacaaggaa ctttaaatat catactaggt ttaattaagt
    22021 tcttctcaag tttatttact ggagattggc gaggagtttg ggacgcgatt attatgattc
    22081 ttaaaggagt cgttcaatta atatggaatt taattcaatt atggtttgta ggcaaaatac
    22141 ttggcgttgt taggtacttt ggcggattgc taaaaggatt aataacaggt atttgggacg
    22201 taataaaaag tatattcagt aaatctttat cagcaatttg gaatgcgaca aaaagtattt
    22261 ttggattctt atttaatagt gtcaaatcaa ttttcacgaa tatgaaaaat tggttatcta
    22321 acacatggag tagtatccgt acgaatacga taggaaaagc acagtcatta tttagtggtg
    22381 tgaaatcaaa attcactagt ttgtggaatg cgacaaaaga tatttttagt aatctaagaa
    22441 attggatggc aaatatttgg aattccatta aagataatac ggttggaatt gcaagtcgtt
    22501 tatggagtaa agtgcgtgga atttttacaa atatgcgtga cggcttacaa agtattatca
    22561 gcaaaattaa aagtcatatc ggcggtatgg tagatgctat taaaaaagga cttaataaat
    22621 taattgaagg tttaaactgg gtcggtggta agctgggaat ggataaaata cctaagttac
    22681 acactggtac agaacacaca catactacta caagattagt taagaacggt aagattgcgc
    22741 gggatacgtt cgctacggtt ggggataaag gacgtggaaa tggtccgaat ggtttcagaa
    22801 atgaaataat tgaattccct aatggtaaac gtgtaatcac acctaataca gatactaccg
    22861 cttatttacc taaaggctca aaagtataca acggtacaca aacttattca atgttaaacg
    22921 gaacgcttcc aagatttagc ttaggtacta tgtggaaaga tattaaatcc ggtgcatcat
    22981 cggcatttaa ctggacaaaa gatcaaatag gtaaaggtac caaatggctt ggcgataaag
    23041 ttggcgatgt tttagatttt atggaaaatc ctggcaaact tttaaattat atacttgaag
    23101 cttttggaat tgatttcaat tctttaacta aaggtatggg aattgcaggc gacataacaa
    23161 aagctgcatg gtctaagatt aagaaaagtg ctactgattg gataaaagaa aatttagaag
    23221 ctatgggcgg tggcgattta gtcggcggaa tattagaccc tgacaaaatt aattatcatt
    23281 atggacgtac cgcagcttat actgctgcaa ctggaagacc atttcatgaa ggtgtcgatt
    23341 ttccatttgt atatcaagaa gttagaacgc cgatgggtgg cagacttaca agaatgccat
    23401 ttatgtctgg tggttatggt aattatgtaa aaattactag tggagttatc gatatgctat
    23461 ttgcgcattt gaaaaacttt agcaaatcac cacctagtgg cacgatggta aagcccggcg
    23521 atgttgttgg tttaactggt aataccggat ttagtacagg accacactta cattttgaaa
    23581 tgagaagaaa tggacgtcac tttgaccctg aaccatattt aagaaatgca aagaaaaaag
    23641 gtaggttatc aattggtggc ggtggtgcta cttctggaag tggtgcaact tatgcaagcc
    23701 gagtaatccg tcaagcgcaa agtattttag gaggacgtta taaaggtaag tggattcatg
    23761 accagatgat gcgagttgca aagcgtgaaa gtaactatca atcaaatgca gtgaataatt
    23821 gggatattaa tgctcaaaga ggagacccat caagaggatt attccaaatc atcggctcaa
    23881 cttttagagc aaacgctaaa cgtggatata ctaactttaa taatccggtt catcaaggta
    23941 tttcagcaat gcagtacatt gttagacgct acggttggga tggatttaag cgtgctggtg
    24001 attacgcata tgctacaggt ggaaaagttt ttgatggttg gtataactta ggtgaagacg
    24061 gtcatccaga atggattatt ccaacagatc cagctcgtag aaatgatgca atgaagatgt
    24121 tgcattatgc agcagcagaa gtaagaggga gaaaagcgag taaaaataag cgtcctagtc
    24181 aattgtctag tgtaaatggg tttgatgacc caagcttatt attgaaaatg attgaacaac
    24241 agcaacaaca aatagcttta ttactgaaaa tagcgcaatc caacgatgtg attgcagata
    24301 aagattatca gccgattatt gacgaatacg cttttgataa aaaggtgaac gcgtctatag
    24361 aaaagcgaga aaggcaagaa tcaacaaaag taaagtttag aaaaggagga attgctattc
    24421 aatgatagac actattaaag tgaacaacaa aacaattcct tggttgtatg tcgaaagagg
    24481 gtttgaaata ccctctttta attatgtttt aaaaactgaa aatgtagatg gacgttcggg
    24541 gtctatatat aaagggcgta ggcttgaatc ttatagtttt gatatacctt tggtggtacg
    24601 taatgactat ttatctcaca acggctttaa aacacatgat gacgtcttga atgaattagt
    24661 aaagtttttt aactacgagg aacaagttaa attacaattc aaatctaaag attggtactg
    24721 gaacgcttat tttgaaggac caataaagct gcacaaagaa tttgcaatac ctgttaagtt
    24781 cactatcaaa gtagtactaa cagaccctta caaatattca gtaacaggat ataaaaatac
    24841 tgcgatttca gaccaagttt cagttgtaaa tagtgggact gctgacactc ctttaattgt
    24901 tgaagcccga gcaattaaac catctagtta ctttatgatc actaaaaatg atgaagatta
    24961 ttttatggtt ggtgatgatg aggtaaccaa agaagttaag gattacatgc ctcctgttta
    25021 tcatagtgag tttcgtgatt tcaaaggttg gactaagatg attactgaag atattccaag
    25081 taatgattta ggtggtaaag tcggcggtga ctttgtgata tccaatcttg gcgaaggata
    25141 taaagcaact aatttttctg atgcaaaagg ttgggttggt gctggcacga aacgagggct
    25201 ccctaaagcg atgacagatt ttcaaattac ctataaatgt attgttgaac aaaaaggtaa
    25261 aggtgccgga agaacagcac aacatattta tgatagtgat ggtaagttac ttgcttctat
    25321 tggttatgaa aataaatatc atgatagaaa aataggacat attgttgtta cgttgtataa
    25381 ccaaaaagga gaccccaaaa agatatacga ctatcagaat aaaccgataa tgtataactt
    25441 ggacagaatc gttgtttata tgcggctcag aagagtaggt aataaatttt ctattaaaac
    25501 ttggaaattt gatcacatta aagacccaga tagacgtaaa cctattgata tggatgagaa
    25561 agagtggata gatggcggta agttttatca gcgtccagct tctatcatag ctatctatag
    25621 tgcgaagtat aatggttata agtggatgga gatgaatgga ttaggttcat tcaatacgga
    25681 gattctacca aaaccgaaag gcgcaaggga tgtcattata caaaaaggtg atttagtaaa
    25741 aatagatatg caagcaaaaa gtgttgtcat caatgaggaa ccaatgttga gcgagaaatc
    25801 gtttggaagt aattatttca atgttgattc tgggtacagt gaattaatca tacaacctga
    25861 aaacgtcttt gatacgacgg ttaaatggca agatagatat ttatagaaag gagatgagag
    25921 tgtgatacat gttttagatt ttaacgacaa gattatagat ttcctttcta ctgatgaccc
    25981 ttccttagtt agagcgattc ataaacgtaa tgttaatgac aattcagaaa tgcttgaact
    26041 gctcatatca tcagaaagag ctgaaaagtt ccgtgaacga catcgtgtta ttataaggga
    26101 ttcaaacaaa caatggcgtg aatttattat taactgggtt caagatacga tggacggcta
    26161 cacagagata gaatgtatag cgtcttatct tgctgatata acaacagcta aaccgtatgc
    26221 accaggcaaa tttgagaaaa agacaacttc agaagcattg aaagatgtgt tgagcgatac
    26281 aggttgggaa gtttctgaac aaaccgaata cgatggctta cgtactacgt catggacttc
    26341 ttatcaaact agatatgaag ttttaaagca attatgtaca acctataaaa tggtattgga
    26401 tttttatata gagcttagtt ctaataccgt caaaggtaga tatgtggtac tcaaaaagaa
    26461 aaacagctta ttcaaaggta aagaaattga gtatggtaaa gatttggttg ggttaactag
    26521 gaagattgat atgtcagaaa tcaaaacagc attaattgct gtgggacccg aaaatgacaa
    26581 aggaaagcgt ttagagctag ttgtgacaga tgacgaagcg caaagtcaat tcaacctacc
    26641 tatgcgctat atttggggga tatatgaacc acaatcagat gatcaaaata tgaatgaaac
    26701 acgattaagt tctttagcca aaacagagtt aaataaacgt aagtcggcag ttatgtcata
    26761 tgagattact tctactgatt tggaagttac gtatccgcac gagattatat caattggcga
    26821 tacagtcaga gtaaaacata gagattttaa cccgccattg tatgtagagg cagaagttat
    26881 tgctgaagaa tataacataa tttcagaaaa tagcacatat acattcggtc aacctaaaga
    26941 gttcaaagaa tcagaattac gagaagagtt taacaagcga ttaaacctaa tacaccaaaa
    27001 attaaacgac aatattagca atatcaatac tatagtaaaa gatgttgtag atggtgaatt
    27061 agaatacttt gaacgcaaaa ttcataaaag tgatacaccg ccagaaaatc cagtcaatga
    27121 tacgctttgg tatgatacaa gtaaccctga tgttgctgtc ttgcgtagat attggaatgg
    27181 tcgatggatt gaagcaacac caaatgatgt tgaaaaatta ggtggtataa caagagagaa
    27241 agcgctattc agtgaattaa acaatatttt tattaattta tctatacaac acgctagtct
    27301 tttgtcagaa gctacagaat tactgaatag cgagtactta gtagataatg atttgaaagc
    27361 ggacttacaa gcaagtttag acgctgtgat tgatgtttat aatcaaatta aaaataattt
    27421 agaatctatg acacccgaaa ctgcaacgat tggtcggttg gtagatacaa aaactttatt
    27481 tcttgagtat agaaagaaat tacaagatgt ttatacagat gtagaagatg tcaaaatcgc
    27541 catttcagat agatttaaat tattacagtc acaatacact gatgaaaaat ataaagaagc
    27601 gttggaaata atagcaacaa aatttggttt aacggtgaat gaagatttgc agttagtcgg
    27661 agaacctaat gttgttaaat cagctattga agcagctaga gaatccacaa aagaacaatt
    27721 acgtgactat gtaaaaacat cggactataa aacagacaaa gacggtattg ttgaacgttt
    27781 agatactgct gaagctgaga gaacgacttt aaaaggtgaa atcaaagata aagttacgtt
    27841 aaacgaatat cgaaacggat tggaagaaca aaaacaatat actgatgacc agttaagtga
    27901 tttgtccaat aatcctgaga ttaaagcaag tattgaacaa gcaaatcaag aagcgcaaga
    27961 agctttaaaa tcatacattg atgctcaaga taatcttaaa gagaaggaat cgcaagcgta
    28021 tgctgatggt aaaatttcgg aagaagagca acgcgctata caagatgctc aagctaaact
    28081 tgaagaggca aaacaaaacg cagaactaaa ggctagaaac gctgaaaaga aagctaatgc
    28141 ttatacagac aacaaggtca aagaaagcac agatgcacag aggagaacac tgactcgcta
    28201 tggttctcaa attatacaaa atggtaagga aatcaaatta agaactacta aagaagagtt
    28261 taatgcaacc aatcgtacac tttcaaatat attaaacgag attgtccaaa acgttacaga
    28321 tggaacaaca atcagatatg atgataacgg agtggctcaa gctttaaatg tggggccacg
    28381 tggtattaga ttaaatgctg ataaaattga tattaacggt aatagagaaa taaaccttct
    28441 tatccaaaat atgcgagata aagtagataa aaccgatatt gtcaacagcc ttaatttatc
    28501 aagagagggt cttgatatca atgttaatag aattggaatt aaaggcggta acaataacag
    28561 atatgttcaa atacagaatg attctattga actaggtggt attgtgcaac gaacttggaa
    28621 aggcaaacga tcaaccgatg atatattcac acgtcttaaa gatggacatc taaggtttag
    28681 aaataatacc gcaggcggtt cactttatat gtcacatttt ggtatttcaa catatattga
    28741 tggagaaggc gaagacggag gttcatccgg tactattcaa tggtgggata aaacttacag
    28801 tgatagcggt atgaatggca taacaatcaa ttcctatggt ggtgtcgttg cactaacgtc
    28861 agataataat cgggttgttc tggagtctta cgcttcatcg aatatcaaaa gcaaacaggc
    28921 accggtgtat ttatatccaa acacagacaa agtgcctgga ttaaaccgat ttgcattcac
    28981 gctgtctaat gcagataatg cttattcgag tgacggttat attatgtttg gttctgatga
    29041 gaactatgat tacggtgcgg gtatcaggtt ttctaaagaa agaaataaag gtcttgttca
    29101 aattgttaat ggacgatatg caacaggtgg agatacaaca atcgaagcag ggtatggcaa
    29161 atttaatatg ctgaaacgac gtgatggtaa taggtatatt catatacaga gtacagacct
    29221 actgtctgta ggttcagatg atgcaggaga taggatagct tctaactcaa tttatagacg
    29281 tacttattcg gccgcagcta atttgcatat tacttctgct ggcacaattg ggcgttcgac
    29341 atcagcgcgt aaatacaagt tatctatcga aaatcaatat aacgatagag atgaacaact
    29401 ggaacattca aaagctattc ttaacttacc tattagaacg tggtttgata aagctgagtc
    29461 tgaaatttta gctagagagc tgagagaaga tagaaaatta tcggaagaca cctataaact
    29521 tgatagatac gtaggtttga ttgctgaaga ggtggagaat ttaggattaa aagagtttgt
    29581 cacgtatgat gacaaaggag aaattgaagg tatagcgtat gatcgtctat ggattcatct
    29641 tatccctgtt atcaaagaac aacaactaag aatcaagaaa ttggaggagt caaagaatgc
    29701 aggataacaa acaaggatta caagctaatc ctgaatatac aattcattat ttatcacagg
    29761 aaattatgag gttaacacaa gaaaacgcga tgttaaaagc gtatatacaa gaaaataaag
    29821 aaaatcaaca atgtgctgag gaagagtaat ctttagcact atttttatac aaaaatttaa
    29881 ggaggtcatt taattatggc aaaagaaatt atcaacaata cagaaaggtt tattttagta
    29941 caaatcgaca aagaaggtac agaacgtgta gtatatcaag atttcacagg aagttttaca
    30001 acttctgaaa tggttaacca tgctcaagat tttaaatctg aagaaaacgc taagaaaatt
    30061 gcggagacgt taaatttgtt atatcaatta actaacaaaa aacaacgtgt gaaagtagtt
    30121 aaagaagtag ttgaaagatc agatttatct ccagaggtaa cagttaacac tgaaacagta
    30181 tgaaaagcta tgagttagat actcataatc tttattcttt tagaaagcgg gtgtactgaa
    30241 ttggggtggt tcaaaaaaca cgaacatgaa tggcgcatca gaaggttaga agagaatgat
    30301 aaaacaatgc tcagcacact caatgaaatt aaattaggtc aaaaaaccca agagcaagtt
    30361 aacattaaat tagataaaac cttagatgct attcaaaaag aaagagaaat agatgaaaag
    30421 aataagaaag aaaatgataa gaacatacgt gatatgaaaa tgtgggtgct tggtttagtt
    30481 gggacaatat ttggatcgct aattatagca ttattgcgta tgcttatggg catataagag
    30541 aggtgaataa aatgtttaaa ctaatctttg gttatagttt ctggacatgt ttttggttcg
    30601 gtaaatgtaa ataagtttta gtcagtgctt cggtactgac tttttattta ttgttgtaat
    30661 tatggtaata tgcagaagtg agcaagttgg atagatggtg gctatctgag tataaggagg
    30721 tggtgcctat ggtggcatta ctgaaatctt tagaaaggag acgcctaatg attacaatta
    30781 gtaccatgtt gcagtttggt ttattcctta ttgcattgat aggtctagta atcaagctta
    30841 ttgaattaag caataaaaaa taaccatcgc taactttggc tggtttcgat ggttaaatgg
    30901 ttattaattt aatctttaat ctaaaatagc caccgtcttt ttaacgggct cattagggta
    30961 acatgtttgc gcatgttgcc ctttttctat atataaatta acacaccata atataaatat
    31021 caaatagacg gcttattagt cgtcttttta ttttgggtaa aaggagataa gaatatgatt
    31081 aattggaaaa ttagaatgaa acaaaaatca ttttgggtag cgatattgtc agctatcttt
    31141 ttatttgctc aaaacatcgc aaaagctatt gggtatgata tccaagttta tacagagcaa
    31201 ttaacagacg gtttaaacgc tatattagga tttttagtat taactggtgt gattcaagac
    31261 ccgactacta aaggtatagg tgatagccac caagctttag aatatgaaga accaagaaga
    31321 aaatactagg aggtaaaata atgaaaacat acagtgaagc aagagcaagg ttacgttggt
    31381 atcaaggtag atatattgat tttgacggtt ggtatggtta ccaatgtgca gatttagcag
    31441 ttgattacat ttattggttg ttagaaatta gaatgtgggg aaatgcaaaa gatgcaatca
    31501 ataacgattt taaaaacatg gcaacagtat atgaaaacac accatcgttt gttccacaaa
    31561 taggtgatgt ggctgtattt accaaaggaa tatataaaca atacggtcat attggtttag
    31621 tgtttaatgg tggtaataca aaccaatttt taattttgga acagaactat gacggtaacg
    31681 caaatacgcc tgcaaagtta cgttgggata attattacgg ctgtactcac tttattagac
    31741 ctaagtataa aagtgagggc ttaatgaata agatcacaaa taaagttaaa ccacctgctc
    31801 aaaaagcagt cggtaaatct gcaagtaaaa taacagttgg aagtaaagcg ccttataacc
    31861 ttaaatggtc aaaaggtgct tattttaatg cgaaaatcga cggcttaggt gctacttcag
    31921 ccactagata cggtgataat cgtactaact atagattcga tgttggacag gctgtataca
    31981 cgcctggaac attaatatat gtgtttgaaa ttatagatgg ttggtgtcgc atttattgga
    32041 acaatcataa tgagtggata tggcatgaga gattgattgt gaaagaagtg ttttaattct
    32101 taggttaaaa tgttaaatat ttgttaatta ttttttaatg taattttagt ttcttttaat
    32161 attttattga tttttaatat tttctcaata taaaatgaag ttgttgatat ttatcatctt
    32221 aaataagggt gttagctata aaaagagata aataaaaaca aatatattat atttggagga
    32281 agcgccatgc tcaaaagagg tttattattt ttaactgttt tattgttatt attctcattt
    32341 tcttcaatta ctaatgaggt aagtgcatca agttcattcg acaaaggaaa atataaaaaa
    32401 ggcgatgacg cgagttattt tgaaccaaca ggcccgtatt tgatggtaaa tgtgactgga
    32461 gttgatggta aaggaaatga attgctatcc cctcattatg tcgagtttcc tattaaacct
    32521 gggactacac ttacaaaaga aaaaattgaa tactatgtcg aatgggcatt agatgcgaca
    32581 gcatataaag agtttagagt agttgaatta gatccaagcg caaagatcga agtcacttat
    32641 tatgataaga ataagaaaaa agaagaaacg aagtctttcc ctataacaga aaaaggtttt
    32701 gttgtcccag atttatcaga gcatattaaa aaccctggat tcaacttaat tacaaaggtt
    32761 attatagaaa agaaataaaa caaaatagtt gtttattata gaaagcaatg tcttgattga
    32821 atatgtgtag tgaaaattat ctttcatcaa attctcattc atgcacgaat ggttcttccc
    32881 cacctaatca gatattaggt gacttatggg gagaaatcag ttaggatgaa aaagtggata
    32941 atcctttttt aggcaggtac ttcggtactt gcctattttt ttatgttata atctttctag
    33001 acgtattcaa gggacgtctt tttagattgt atgttatagc tagctttcgg gctagttttt
    33061 tgttatgatg tgttacacat gcatcaacta tttacatcta tccttgttca cccaagcatg
    33121 tcactgggtg ttttttctta tgatagagag catagttttc atactactcc ctcgtagtat
    33181 atatgacttt agcattcccg tataatagtt tacggggtgc tttttatgtt ataattaact
    33241 gtatatagta ggagtgaact atatagcctg ttaagtggcc tagtaaccta acacttatcc
    33301 tgcaattgat atcctttttg cccttcactc gatacatata tctcaacaac atagaaatat
    33361 tacagtcgct acaccgcatc ttaaatggtg tggttatttt tattggaagt gtgtatcagg
    33421 tatcagtaat gttaaaacac cagctaaaaa tgaaaagaat tcaccagtgc cagcaggtta
    33481 tacactcgat aaaaacaatg taccgtataa aaaagagact ggttattaca cagttgccaa
    33541 tgttaaaggt aataacgtga gggatggcta ttcaactaat tcaagaatta caggtgtatt
    33601 acccaataac gcaactatca aatatgacgg cgcatattgc attaatggct atagatggat
    33661 tacttatatt gctaatagtg gacaacgtcg ttatatagcg acaggagagg tagacaaggc
    33721 aggtaataga ataagcagtt ttggtaagtt tagtgcagtt tgataattag atatataaag
    33781 gtttggcaag ttatgaaatg tctgccaaac ctttatataa aaaagaaata tctacctttt
    33841 aatttatgta actactatta gtatgcatat tcattagttt ttccaggacc attaattaca
    33901 taagatgatt tagactctcc ttttttaaag aagtatgttt tatacatttt acctagtaac
    33961 tcaacatttt ttctatcttc agcaagtggt gtattcagat atactgtata gtaaccttta
    34021 ttttcagtta aaataaccat tttttcaaat tgagcagaat tttttgtgcc tttctttaaa
    34081 taatttctca aacgttcatc taattttcct agcgttgtag gaagaccact atttttaaat
    34141 gattctttat aagctttttc tttctctaac attttcttat ttgattctat ttcttcattt
    34201 gtaggaaacg gttcaaaagt aaaagctttc gctgaatgat ggtgtgtact gattcctgcc
    34261 gttaaaaaac ttaatgctaa aactgttgtt gctaatttct ttttcataat gatgttaatt
    34321 ctccttaaat tctatattta aatttatagt tagttttgcg aaattcctaa aatgagttta
    34381 atctaatcga cgaaatatat taattaactt gaaattaata aaagattaat tatttttaac
    34441 taaaaattaa aattcaatta gtgttttgat gaatttggtc tcgataaatt gaaataatct
    34501 aaaaaacgct ataatttttc tattaatagt aattaatatg tgctatattt ttcttagaca
    34561 cagcaatgtg ttcaaatttt catctattca taagctagcc ttcgggctag tttttttgtg
    34621 ctatatatat ttgttttaat taaataaaat tagataatgc aatagtagcc attttatgtt
    34681 aatattacct tgggcgtttt caaggagcgc ctttcatttt ttatgtattg ctccccttcg
    34741 ggctagtata ttaaatttat ttttgcgctt tccaaatcaa tgtatatgtg ttatattgtt
    34801 tatgggaagt aggtaagcat ttcggtgctt accttttttt gtttttctat aaatacaata
    34861 aggtatgtca atttgataat ttattaattt tcatttaata agaagatcta tatagttaat
    34921 gaataattaa tgtacttttt tttagtcagt cattaaaata aattagtact aattactaag
    34981 gagaataaaa aatgaaaatt agaaaatcta tacttgcggg aactttagca atcgttttag
    35041 catcaccact agtaactaat ctagataaaa atgaggcaca agctagcaca agcttgccaa
    35101 catcgaatga atatcaaaac gaaaagttag ctaatgaatt aaaatcgtta ttagatgaac
    35161 taaatgttaa tgaattagct actggaagtt taaacactta ttataagcga actataaaaa
    35221 tttcaggtca aaaagcaatg tatgctctta agtcaaaaga ctttaagaaa atgtcagaag
    35281 caaaatatca acttcaaaag atttataacg aaattgacga agcactaaaa agtaaatatt
    35341 aaaaaaacca cccttttacg ggtggtttta attttctaga taatataaaa gtgttcataa
    35401 ataaaacagt ataggcaaac aataaagtat tgaaaaaagt aagtttaata tgaaaattgt
    35461 taaatgaacg acatcttttg tttttataaa tatcaagaga a
//
LOCUS       DAKTLH010000013        33718 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.013, whole genome shotgun sequence.
ACCESSION   DAKTLH010000013 DAKTLH010000000
VERSION     DAKTLH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 33718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33718
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.013"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            11..649
                     /locus_tag="O0394_000568"
     CDS             11..649
                     /locus_tag="O0394_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HCY0498671.1"
                     /translation="MYKINIIRSDSVYNNILKAPINDRDSIFTKEILVPFKKKFEVQH
                     MPIYNDDKQTMSAIQFLDAFQISPKDLRMSDQMSIQYLNNDFWSNCEKYLKVAIDQFS
                     NYSISSQVSNYHFTVLLGDRQKPLMYLNKNRGGDGGIPGYIMIYLVPSTSTINSMKSL
                     IAHEVNHNMRYQYIDWDGGSLIELIIAEGLAENYVESLYGKAHIGPWVTNTN"
     gene            668..925
                     /locus_tag="O0394_000569"
     CDS             668..925
                     /locus_tag="O0394_000569"
                     /inference="COORDINATES: protein motif:HMM:NF021537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498672.1"
                     /translation="MKNTIYNHLHLKHIFESMPYLYGDDINKLQGRPIVGLSHAAGYA
                     CGYHLVKYFLQKTNIPIEVATTLPAQKIINEVTEFWHTHTL"
     gene            904..1644
                     /locus_tag="O0394_000570"
     CDS             904..1644
                     /locus_tag="O0394_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0498673.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQISKLDKKISDLQLIRRSV
                     CEFIKGLSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTT
                     IFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(1902..2555)
                     /gene="nreC"
                     /locus_tag="O0394_000571"
     CDS             complement(1902..2555)
                     /gene="nreC"
                     /locus_tag="O0394_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HCY0498674.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(2578..3612)
                     /gene="nreB"
                     /locus_tag="O0394_000572"
     CDS             complement(2578..3612)
                     /gene="nreB"
                     /locus_tag="O0394_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HCY0498675.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            complement(3636..4088)
                     /gene="nreA"
                     /locus_tag="O0394_000573"
     CDS             complement(3636..4088)
                     /gene="nreA"
                     /locus_tag="O0394_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HCY0498676.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            complement(4108..4785)
                     /gene="narI"
                     /locus_tag="O0394_000574"
     CDS             complement(4108..4785)
                     /gene="narI"
                     /locus_tag="O0394_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCY0498677.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKPWMDAVGVSEHLYHIGAVYIGSIFGIITLI
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            complement(4778..5353)
                     /gene="narJ"
                     /locus_tag="O0394_000575"
     CDS             complement(4778..5353)
                     /gene="narJ"
                     /locus_tag="O0394_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCY0498678.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            complement(5346..6905)
                     /gene="narH"
                     /locus_tag="O0394_000576"
     CDS             complement(5346..6905)
                     /gene="narH"
                     /locus_tag="O0394_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCY0498679.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            complement(6895..10584)
                     /locus_tag="O0394_000577"
     CDS             complement(6895..10584)
                     /locus_tag="O0394_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCY0498680.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILQRAIDEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            complement(10893..11870)
                     /gene="cobA"
                     /locus_tag="O0394_000578"
     CDS             complement(10893..11870)
                     /gene="cobA"
                     /locus_tag="O0394_000578"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCY0498681.1"
                     /translation="MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVD
                     VNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNII
                     SKLQHYSIEAKPGICIIGEVVDYTENTPKSYDPMKQFYVVSGSKHDALMLCEHLYDEG
                     YGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
     gene            complement(11861..12175)
                     /gene="nirD"
                     /locus_tag="O0394_000579"
     CDS             complement(11861..12175)
                     /gene="nirD"
                     /locus_tag="O0394_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCY0498682.1"
                     /translation="METKEKIKVTTIDELTPLIGKKVIVKGKEVGLFLTESGKIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            complement(12179..14584)
                     /locus_tag="O0394_000580"
     CDS             complement(12179..14584)
                     /locus_tag="O0394_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0498683.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTLDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARRIFEV
                     EKV"
     gene            complement(14650..15381)
                     /locus_tag="O0394_000581"
     CDS             complement(14650..15381)
                     /locus_tag="O0394_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HCY0498684.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGDEQQVGVMVVAHGNINGKFTKAHEELQICAQNLQISKPT
                     YARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHFTP
                     SINFDPILKQIINDRLESLMIPMKI"
     gene            complement(15611..16147)
                     /locus_tag="O0394_000582"
     CDS             complement(15611..16147)
                     /locus_tag="O0394_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0498685.1"
                     /translation="MTKTMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            complement(16380..17204)
                     /locus_tag="O0394_000583"
     CDS             complement(16380..17204)
                     /locus_tag="O0394_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCY0498686.1"
                     /translation="MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMY
                     FTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(17421..17603)
                     /locus_tag="O0394_000584"
     CDS             complement(17421..17603)
                     /locus_tag="O0394_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498687.1"
                     /translation="MVERYIKVLILYIFTTLLSSISVTSKCVPNKVIRFILRTAIGYS
                     VFAYGLHYFSNLKKNK"
     gene            complement(17687..18154)
                     /locus_tag="O0394_000585"
     CDS             complement(17687..18154)
                     /locus_tag="O0394_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCY0498688.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(18340..19890)
                     /gene="adcA"
                     /locus_tag="O0394_000586"
     CDS             complement(18340..19890)
                     /gene="adcA"
                     /locus_tag="O0394_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HCY0498689.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHHGGYDPHVWLDPK
                     INQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISH
                     ESIGYLADCYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTET
                     IRKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAES
                     KHDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKD
                     LKAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFK
                     LVDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQ
                     IKEEMLAH"
     gene            complement(20079..20150)
                     /locus_tag="O0394_000587"
                     /pseudo
     CDS             complement(20079..20150)
                     /locus_tag="O0394_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20185..20451)
                     /locus_tag="O0394_000588"
     CDS             complement(20185..20451)
                     /locus_tag="O0394_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCY0498690.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALE
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(20451..20702)
                     /locus_tag="O0394_000589"
     CDS             complement(20451..20702)
                     /locus_tag="O0394_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCY0498691.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSVMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            complement(20867..20968)
                     /locus_tag="O0394_000590"
     CDS             complement(20867..20968)
                     /locus_tag="O0394_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498692.1"
                     /translation="MLRSVSDTNLLGQYEKAISISDMIEIVFLMYEV"
     gene            complement(20975..21574)
                     /locus_tag="O0394_000591"
     CDS             complement(20975..21574)
                     /locus_tag="O0394_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCY0498693.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVVYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(21593..21952)
                     /locus_tag="O0394_000592"
     CDS             complement(21593..21952)
                     /locus_tag="O0394_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCY0498694.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDEVHYYAYDFSDKRVSYKQDFDSR
                     RYYQQHDADYHEENMTN"
     gene            22207..23457
                     /gene="fmhA"
                     /locus_tag="O0394_000593"
     CDS             22207..23457
                     /gene="fmhA"
                     /locus_tag="O0394_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HCY0498695.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKHNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDKLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPLLYKVQTYLNHKRR"
     gene            complement(23554..24285)
                     /locus_tag="O0394_000594"
     CDS             complement(23554..24285)
                     /locus_tag="O0394_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCY0498696.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGMIGEQ
                     GAPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(24282..25001)
                     /locus_tag="O0394_000595"
     CDS             complement(24282..25001)
                     /locus_tag="O0394_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCY0498697.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(24982..25761)
                     /locus_tag="O0394_000596"
     CDS             complement(24982..25761)
                     /locus_tag="O0394_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCY0498698.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(25882..27321)
                     /gene="mdeA"
                     /locus_tag="O0394_000597"
     CDS             complement(25882..27321)
                     /gene="mdeA"
                     /locus_tag="O0394_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HCY0498699.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVVSFFCSFMLQGKKKEVDSRQ"
     gene            27495..27599
                     /locus_tag="O0394_000598"
     CDS             27495..27599
                     /locus_tag="O0394_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCY0498700.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(27753..28439)
                     /locus_tag="O0394_000599"
     CDS             complement(27753..28439)
                     /locus_tag="O0394_000599"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCY0498701.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(28766..29632)
                     /locus_tag="O0394_000600"
     CDS             complement(28766..29632)
                     /locus_tag="O0394_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCY0498702.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
     gene            complement(30185..30289)
                     /locus_tag="O0394_000601"
     CDS             complement(30185..30289)
                     /locus_tag="O0394_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498703.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKEVLYGEMQ"
     gene            30384..31694
                     /gene="sbi"
                     /locus_tag="O0394_000602"
     CDS             30384..31694
                     /gene="sbi"
                     /locus_tag="O0394_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein Sbi"
                     /protein_id="HCY0498704.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNEKDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKVESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNTYYKYKGAIDQTVLTVLGSGSKSYIQPLKVDDKNGYLAKSYAQVRNYVT
                     ESINTGKVLYTFYQNPTLVKTAIKAQETASSIKNTLSNLLSFWK"
     gene            32230..33159
                     /gene="hlgA"
                     /locus_tag="O0394_000603"
                     /pseudo
     CDS             32230..33159
                     /gene="hlgA"
                     /locus_tag="O0394_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
BASE COUNT        10943 a         6459 c         4759 g        11557 t
ORIGIN      
        1 gaggtttatc atgtataaaa tcaatattat tagatcagat tccgtctata ataatatact
       61 caaggcacca ataaacgacc gtgattcaat tttcactaaa gaaatattag taccttttaa
      121 aaagaaattt gaggtacaac atatgcctat ttataatgat gacaaacaga caatgagtgc
      181 gattcaattt ttagatgcct ttcaaatttc tcccaaagat ttacgcatgt ctgaccaaat
      241 gtcaatacaa tacctgaata atgatttctg gagtaattgc gaaaaatatt taaaagttgc
      301 tatagatcaa ttttcaaatt atagtatctc atctcaagtt tctaactatc atttcacagt
      361 attacttgga gatcgccaaa aaccacttat gtatctaaat aaaaatcgcg gtggtgatgg
      421 tggcatacca ggttatatta tgatttattt agtgccgagt acaagtacaa ttaattctat
      481 gaaaagctta attgcacatg aagtaaatca taatatgcgc tatcaatata ttgattggga
      541 tggcggaagt ttgattgaat tgattattgc agaaggatta gctgaaaact atgtagaatc
      601 attgtatggt aaagcgcata ttggaccgtg ggtaacaaat actaattgaa gtcgtgataa
      661 cgtaaaaatt aaaaatacta tttataatca tttacattta aagcatatat ttgaatcgat
      721 gccttatctc tatggtgatg atattaataa acttcaaggt aggcctatcg ttggcttatc
      781 tcatgctgcc gggtatgcat gtggctatca cttggtaaaa tactttttac aaaaaacaaa
      841 catacctatt gaagttgcta caacacttcc agcacaaaaa ataataaatg aggtgactga
      901 attttggcat acacatacac tttaaaagat attattgaaa ttacaggtgt aactaaaaga
      961 actttacatt attacgatga aataggatta ttagttccag ataaaaatga taaaaattat
     1021 cgcgtttata aacagcaaga cttagaaaaa ttacaaaaga ttttaatact caagtctttt
     1081 gattttgata tcgctaaaat aaaacaatac atttcgtatg ataatgaaca attgcgaaag
     1141 ttattatcag agcaaataag caagttagat aaaaagattt ctgacttaca attaattaga
     1201 cgctctgtat gtgaatttat taaaggactc tctctaatag ataccagcat tttaaacaag
     1261 acactacagt cacaatatga taaagaagca tctataaaat atggtcatac gaaagcatat
     1321 caatcattta ttagacgtaa agacagctta caatcgcagg atatcagaca taaattgaca
     1381 actatcttca ataaatttaa tcatatgtct ttgagtcatt atccaatcca agattgtagt
     1441 gatctcgtat ttgagtggaa ggcatttatg aacactatcg ctgattttga tgatgaaaca
     1501 ttatgctgta ttgctaaaac atatgaagat gatacgcgtt tcaaagatta ctttaattca
     1561 tatgataatc aaaatttagc atcatacatt tcagaagctg ttaattattt tttgagcaat
     1621 gtgaataaga gcgacaattt ttaatcctca tacagatgct acaacactga atacaccaac
     1681 taaaataatt gttcaaagta gaaaactaat catttattct ctaaatcaat aactatatta
     1741 aaagttatac ctttgcaaag cgaattagta taggttaccg aaaggagaaa ggattaggtt
     1801 ccattcgatt tataaaaaaa tatatttcga aacatacaaa agcgccagaa gataatcatt
     1861 tcctctcgcg cttttatact gaattatatt aatttcaaac tctaaaactc taataatttc
     1921 tttttcaatg catattcaac tagctcaggt ttactcttta agccaagctt tgtcataata
     1981 tgcgtcttat gtgcttctac tgttttcaca gatacaaata atttctctgc aatttcttta
     2041 ttcccgtaac ctttggcaat aagtggcaat atttctagtt ctcgttttga taagatttta
     2101 aaaggatctg ttgtgtttgc agtgtcctga tttgaattag atacaaattc attcactaaa
     2161 gatgttgtaa gtttcatatc tacatatgtt tcacctttat atacagttcg aatagctaac
     2221 aataattgtt catcaggtgc atttttcaat atgtaacctt tcgcaccatt acgcaacaca
     2281 tggaacaaat actcctcatc atcaaacatt gttaatatta gtattttagt ttcaggaaaa
     2341 ctgtcagcaa ttttactcgt agcgataaga cctgactcac ctggtggcat acttaaatcc
     2401 attagtaaca catcaggttt atattccatt actttttggt aagcttcgac gccatctgca
     2461 gccgttgcaa caacttccat atcattttga taatttaaaa tcatagagaa ccccgtacgg
     2521 acaacagcgt gatcatcggc aatgactatt ttcaatttta ttcccccaat gtatgtttca
     2581 aattggaatg ttcaatgtaa cattggtacc ctcaccaatt ttcgtttcaa tattgacgct
     2641 accgctgact aactcagctc gctcattcat tccatataaa ccgagtccag aacctttagg
     2701 cttagaactt ggatcaaaac catttcccgc atctatcact tctgctacca aatggcgccc
     2761 agtttgacgg atacctacat ttatttcatt tacatcagcg tatttcaacg catttaaaat
     2821 agcttcttgc actactcgat aaacaaccgt ttcaatatca ctatcaaagc gagtattttt
     2881 aatatttgat gtatatatga tttttattcc ataattttct tcaaactgtt taaaatatga
     2941 tttaaaagct gcttcaaggc ctagatcatc caaagaagcg ggtcttaatt caaccgacat
     3001 attacgtata tcatcaatta atttagcgac aatatattca atattttctg cgtcttccaa
     3061 aagcttagtt gtatcttctt gatattttaa taatctcaat tgaacatcta cattgagcat
     3121 ttcttgaatc acactatcat gtaactctct agaaattcgc tttctttcat tttcttgggc
     3181 tgagattgtt ttacgcatca tacgttgttg atgcaatttc tcttgctgtt caatttgtga
     3241 tgaaacattt tgaagcgtaa atgcatgaat tcccctgtct tgatcaatca actgatatgt
     3301 tgctgtaaat ggcatcactt tttgatcttt cgtcttcata aatacttgga aattcgtagc
     3361 ttgtacttgc atcgattcta agaaacagtc tttacatgat tgtacatcat aggcatttgt
     3421 atacccttca catctgtgac aaatcgcatt cgccacagca ctgtaattat cttcttcaga
     3481 taatatatct ttagcagcat cattcattgc aataatttta ccgttatcat cagcaaaaac
     3541 tatcttttcg attgaatgct cataatattt tttcaataaa gtatctaact gtatactgtc
     3601 ctcattaatc atgacttaca ccctaattca tctcattatt tatcatcatt gaaaatacca
     3661 aacttacgtt gaatatcatc attatcaaat atttttggta aaggacgacc atctctttga
     3721 ccaaataata gtacgccata cacttgattc ttataccaaa gcggcactgc taaaactgct
     3781 gttaatgatt cgctcaataa aattggatag tcaatctttt cttcaggccc taaagctaaa
     3841 ccaacattgg ctattaccat acgctttcct gttttcataa cagttccagc taatccacga
     3901 ccttttctta aaataatcaa tttaaatcga ttatttttat tacctgaaac atagtgccat
     3961 tttattggag atgatggttt gttagattca tagaaagcga ttgccgcaaa atcataaccc
     4021 tcttctttgc gtattttatc taatgtctct tgaaatctac gatcttcaat tattgcttct
     4081 ggtgtcaaat cctttcacct cttatgctta cactttattc ttacggtaaa taatatatct
     4141 gcgatttata tatgtcaaag gtacactcca aacatgcacc aaacgtgtaa atggccaaca
     4201 agccataata gtgaaaccta acaatatatg cattttaaat gcaatcggca caccactcat
     4261 caatgacgca tctggtttta acataaataa ttgtctaaac caaattgata atgaagttct
     4321 gtagttaaag tctggatgtt gtatatttgt tactaatgtt gcgtaacatc ccataaatac
     4381 gataagtaat aataagaaat ttacaaatat atccgacgct gaacttaatc ttcgaatact
     4441 tttcgtagta acacgtctcg ctgttaataa aaacatccct atcaaagtta ttataccaaa
     4501 gatgctacca atataaacag cgcctatatg atataaatgc tcagacacac ccactgcatc
     4561 catccatggt ttcggtatta acaatccaac tacgtgtcca aaaaacactg gaataatacc
     4621 taagtgaaat aataaacttc cccacatcaa cctttttctt tctattaatt cactagattt
     4681 agctgtccaa gaaaatttat cataacgata acgtgcaata tgacctgcga caaagacaac
     4741 taaacataaa tacggaaata taacccataa aaactgatta agcatgatgt ttcactcctt
     4801 ttggtgatgt caaacataat ttcaatgttt ttctaagtgc ttgaatcaca taggcatatg
     4861 gattgttatc ttcaccaagt gcattcgcca tcacatatgt tccatcctca ataatcataa
     4921 tgattaattg aatattctct tcagctcttg gatcatttcg ccattctgcc acttgcaaaa
     4981 attgaagcat caacggtaga taatcagaaa gttcattatc taccatttct agtccaaaca
     5041 tttcatataa tacctttaat ttagctaaca tttgcccacg ttctttttgc gtatcaaatt
     5101 tgttatacgt catatataat ggtgcttttt tcgtaaaatc aaatgtatct gtataaatcg
     5161 ctttgatttc tgataatgaa aatgtcatca ttacatttct aaatgctact aaatcttcat
     5221 atgctggatg atctttcgaa attgtttctt caaaagtttt tggatgaaaa gttaattttt
     5281 ctggaaaaca taactgttgt gccatatatc caaaactttc ttgatatttt ttaaaattat
     5341 cgaaattaat cacggaaaat ccctccatag aaattctcat tataaatttc ttgaccagtt
     5401 ttccctgaac ctactgcaac gccacagcct tcacagttat ctccaaaatg ctcgccgccg
     5461 taattgtatc ctgtactacc ttgtgcgtga tacgtatcta aataggtttc tttgtgtgat
     5521 gttggaataa caaatcgatc ttcatatttg gctagtccta ataaacgata catgtcttta
     5581 gtttggcgct cggttatacc taatcgctct aatcgagacg tgtcaaatgg ctgttgagta
     5641 acttgagatc tcatataact tctcatcatt gccatacgtt gtagggctcc ttttactggc
     5701 tctgtatctc ctgcagtgaa aatattagct aagtattcaa taggtaaacg catttcttca
     5761 atggctggga aaatcgcatc tggattttga gttgtatttt taccttcaaa atagctcata
     5821 attgggctaa gtggtgggca ataccaaacc atcggcatcg ttctaaattc aggatgtaac
     5881 ggaaatgcaa gtttatattc aattgctaac ttataaattg gagagttttg tgcagcttca
     5941 atccaatcgt aaccaatacc atctttttca gcttgagcaa tgacttcttc gtcaaatggg
     6001 tttaagaata tatctaattg tttttcatat aaatctttct cgtctactgc tgaagctgct
     6061 tcatgaactc gatctgcatc atataataaa acacctaagt aacgcatacg tcctgtacaa
     6121 gtttcagagc ataccgtagg catacccgcc tcgattctcg ggaaacagaa agtacacttt
     6181 tcagctttgt tcgttttcca attgaagtaa actttcttat atggacaacc tgtcatacag
     6241 taacgccatc cacgacatgc gtcttggtca actaatacaa tgccatcttc atcacgttta
     6301 tacatagcac ctgaaggaca cgatgcaacg caacttggat tcaagcaatg ttcacataaa
     6361 cgtggtaaat acatcataaa agtttcgtca aattggaatt taatatcttc ttctattttt
     6421 tggatgttag gatcttttgg acctgtaaca tgaccacctg ctaagtcatc ttcccagtta
     6481 ggtccccatt caatttcaat gttatccccc gtaatttctg aatacgctct agcaactggc
     6541 gaatgcttcc ctgatttcgc agttgttaaa tgttcataat tatagttcca tggctcataa
     6601 taatctttaa ttaatggcat atctgggtta taaaaaattt tacctaaagc aatttttgaa
     6661 attctacttc cagattttaa ttcaagtttc cctttacgat ttagtaccca accacctttg
     6721 tagtgttctt ggtcttccca acgtttcgga taccctacac ctggcttcgt ttctacgttg
     6781 ttgaaccaca tgtactcagc acctggacga tttgtccaag tgtttttaca tgtcacacta
     6841 cacgtatggc atcctatgca tttatctaaa tttaatacca tcgcaacttg cgctttaatc
     6901 ttcaagccaa ttaacctcct tcatctttct aactgctaca tataaatccc tttggttccc
     6961 aattggtcca taataattaa agtgataact aatttgtgcg tatcctccga ctagttgtgt
     7021 tggtttcaaa tggattctag tcggcgcgtt gtgtgaacca ccacgtgtat ctgtaatttc
     7081 tgacccaggc gtttgaatat gtttatcttg tgcatgatac ataaacattg tacctttagg
     7141 catacgatgc gaaataactg ctcttgccgt tacaacacca ttacggttat acacttctag
     7201 ccaatcatta tcttggatat cgtgtttttc agcatcttca tttgatatcc aaaccgttgg
     7261 accacctcta aatagtgtca acatatgctt attatcttga tacattgagt gtatattcca
     7321 ttttccatga ggcgttaaat aacgcagtac caaagcatct gtaccacctt taattttctt
     7381 atctctattc ccaaatacca ttggcggcaa tgtcggttta tatactggta agctctcccc
     7441 aaattgttgg aaaacttcgt gatccacata ataactttga cgtcctgtta atgttctaaa
     7501 aggtactaga cgttctatat tcgttgtaaa tggtgaatat cgtcgacctt gtttatttga
     7561 acctgggaat actgctgtcg gtattacttc tcgtggttgt gaagttatat ttaaaaacga
     7621 aattttctca gcagcgcgtt cgctagaaat atcttttaac ggcattccag tttgttcttc
     7681 gagatcttca tatgattttt gtgataattt accattcgta gcagatgaaa tacttagtat
     7741 tgcatcagct acattacgtg ctgtatcaat acgtggacga ttcgctctca cagaatcatc
     7801 atttgtatca ctccacgtac ctaacatact ttttaattct tcatattgtt cactgacacc
     7861 gaaacttaca ccatgtgctc caactttccc tttttcaagt acaggaccaa gcgtgacata
     7921 tttgtcgtaa attttagtgt agtcgcgttc tacaattgca aagttaggca ttgtacgtcc
     7981 aggtaccgct tcaatttcac ccttcgacca atctttcact acgccgtatg gtgttgaaat
     8041 ttcttgcttt gtatcatgac taagtggagt tgtcacaaca tctttaaacg ttccaggtaa
     8101 atagtctttt gccatttctg aaaatgcttt tgccaacgtt ttataaatat cccagtctga
     8161 acgcgattcc cataacggat caatggcagg attgaaagga tgtacatatg gatgcatatc
     8221 cgttgatgat aaatcatgtt tttcatacca agtcgctgcc ggcaaaacaa tgtcagaata
     8281 taacggtgtt gccgtcattc tgaagtctaa agagaccact aaatctaact tacctgttgt
     8341 ttcttcacgc cacgtaattt cttctggctt ttcatcttca tttggtgtag ctaataaccc
     8401 tgattttgtg ccaagtaaat gcttcataaa gtattcttga ccttttgcag aacttgaaat
     8461 taagtttgaa cgccatataa ataatgattt tggatgattc tttttcaaat caggatcttc
     8521 tattgcaaat tgtgtttgtt ttgatttcac ttcatcaatt gcacgttgca aaatcgcttc
     8581 atttgaatct ataccttcat ctttagcttc ttctgcaaac aacaaactat ttttattaaa
     8641 ttgtggatat gatggtaacc aaccaagtct agctgctaaa acattataat cagctggatg
     8701 ttgatgcttt aactcctctg ttttagctaa tggagatttt aaacgatcta catttgactc
     8761 ttcatatttc cattggtctg ttgcaaaata gaaccaactt gttccgtttt gcaaacgtgg
     8821 tggtccttgc cagtctttcg caaatgcgac agtactccat ccttcaatcg gacgacattt
     8881 ttcttgtccc acatagtgag cccaaccgcc accgttcaca ccttgacagc cacataacat
     8941 aactaagttt aagattgaac gataaatcgt atctgagtta aaccaatggt taatacccgc
     9001 acccatgata atcattgaac gcccttcagt atcgatagcg ttttgcgcaa attctttcgc
     9061 tacttgaatg acaacacttt gttttacgcc tgaaatggct tcttgccaag caggtgtata
     9121 ttttgattct gcatcgtcgt atccttttga ttctaattta tgatcaaaac gacgcacgcc
     9181 atattgactt gccattaagt caaaaattgt agcaatacgg actttgtcac catttgctaa
     9241 agtgacttgt cgagttggaa ttggacgatt gaatatccca tctccatcac tatcaaagta
     9301 tgggaattga attgtttcta attcgtatcc accttctgtc attgataatg tagggttaat
     9361 tttagaacca tcttctgttt ctagttttaa gttccacttc ttaccttctt cccaacgttg
     9421 acccattgtg ccattaggta ctactaaact atcgctgatt gcatcatgaa taactggctt
     9481 ccattcgcct tgctctgttg tttgacctaa gtcactcgct cttaaaaatc gacccgcttt
     9541 atatccattt tcatcttcat ccagcatgat aagaaacggc atatctgtat attgtttagc
     9601 gtaatttata aagcgttcat taggttgatt aacataatgt tcttgtaaaa taacatgcgt
     9661 cattgcttgt gcaattgcag catctgaacc aggattcggt gctagccagt tatctgcaaa
     9721 tttcacattt tctgcgtaat ctggtgctac tgaaatgact tttgtacctt tatagcggac
     9781 ttcagtcata aaatgtgcat ccggagtacg tgttaaaggt acattagagc cccacataat
     9841 aatgtatgat gcgttatacc agtcacttga ttcaggcaca tctgtttgct ctccccaaat
     9901 ttgtggagag gcaggtggta aatctgcata ccagtcataa aaactaagca tttcaccacc
     9961 aagcaaattg atgaatcgag cacctgctgc ataactaatc attgacatcg ctggaatagg
    10021 tgtaaatcct gcgattcgat ctggaccata tttttttatt gtatacagta attgtgctgc
    10081 gattatctct gtaacgtctt tccaatttga acgcacgtgc cctcccatac ctcgggcttg
    10141 cttatattgt ttggctttgt cttcattttc aacaatagac gcccatgcag caacgcgatt
    10201 accattgttt tcttctaatg cttcagtcca taaatcccag agttttccac gaatatatgg
    10261 atatttgatt cgaagcggac tgtattcata ccaagagaat gacgcacctc gtggacatcc
    10321 tctcggttca tattcaggca tatccggacc acaacttgga tagtcagttt gttgattttc
    10381 ccaggtaatc acaccatttt tcacaaatac tttccaagaa catgagcctg tacagttaac
    10441 accatgtgtt gttcttactt ctttatcgtg gctccaacgt tctctgtaca ttttttccca
    10501 ttctctactt ttactttcta ggatcgacca attcccatta aatttttctg ttggcttaaa
    10561 gaaattcaat ccaaattttc ccatatttat atcctcctac gtataaaaat acgatgtgta
    10621 gatgtcgtgt ttttaaatac tttaaaatgc ccaagactat tgctttaatt agattgtaca
    10681 ttttttcaca aacataaaat attagggaat cacctaatta cttaaggaat ttccctatca
    10741 ataacgggat ttcattgaaa taatacacaa tcatgtatgg tcatgcttat tgccaatcta
    10801 aatcgttcaa atttggcaca acgacaaata aggcttcaac acgaatatat tctctcggtt
    10861 gaaaccttac ttattcattt attttttata aattagtgac ataacactgt attagcatct
    10921 gcacgatcgg ttgaaatata tgttacattt tcttgctgct taataaatgc atcatagtaa
    10981 tcatattgcg acgaatgata tgtgccattc gatgtatcat ttgggtttag caaacagcca
    11041 taaccttcgt catataaatg ttcacagagc ataagggcgt catgtttaga accacttact
    11101 acataaaatt gcttcatagg atcatatgat ttaggagtgt tttcagtata atcaacaact
    11161 tcccctataa tacatatacc tggtttcgcc tcaattgaat agtgttgcaa ttttgaaata
    11221 atattactta aacgcccctt aacaacaaac tcgttaaaac acgatgcttg aaagacaatc
    11281 gctatcgggt aatcaatatc tgtgtattgt tgtatctgtg tgataatttt ccctaaacgt
    11341 tttaccccca tataaattgc taacgtgcca ccattcacta aggaattgac atccacttca
    11401 ttttcttctg aatctttaaa gtgacctgta gaaaatgtca cacttttagc aactgtacgc
    11461 attgtcaaac ctgtctgcat agtagcaact gctgcgctcg ctgatgtcac ccctggtaca
    11521 atttcaaacg caatatgatg ttcatttagt gtgtcgactt cttcttgcac acgaccaaat
    11581 atcgctggat cgccaccttt aagtctaaca accttgttat atcgacgcgc tgcttccacg
    11641 atacagtcat ttattttttc ttgctgaata tgttttgcat acggcttttt accaacatcg
    11701 ataatttcag tagtcaaatt cgcatattgt aaaattaacg gattcactaa tcgatcatat
    11761 agaatgacat ccgcttcacg tattaaacgc tcagcctttt tcgtcaaata attcggatta
    11821 cctggacccg cacctatcaa gtaaaccttg ccatattcct ctacagacat atatatacgt
    11881 tcccgtctgt aacttctacc tcataaacat ctacacaacc ttcatcaggt tcttgaacaa
    11941 tacctgtatt taaatcaatt ttttgatcgt ggagcgggca aaatacatat tccccactca
    12001 ctgtcccttc agacaatggt ccttgtttgt gtggacagat attgtgaatc gcatgaattt
    12061 tgccactttc tgttaaaaac aaccctacct ctttgccttt gacaataacc ttttttccaa
    12121 ttaggggtgt taattcatct atagttgtca ctttaatttt ttcttttgtt tccatgtatt
    12181 acaccttctc cacttcaaaa attctacgtg cttgcgcatt gctagttatt gcttcccaag
    12241 gttcagcttc gactgctttt ttagcatcca taatgcgttc aaatagttca ttttgtcttt
    12301 ctgggtcaag taagacttct tttacatttt caaatccaag tcttcttaac catggcgctg
    12361 ttctttcagc atatatacct gtttcgcgat aatattgcat caaagcacca cataatttga
    12421 ttacttcatc ttctgtttct acagttgtta aaaattcagc tttttcaact tctgtaccac
    12481 cattaccacc gatatagatt tggaatccat tttcaactga gataatacca aaatctttaa
    12541 cacctgattc aacacaactt cttgggcagc ctgatacacc cattttgaat ttatgaggtg
    12601 tatcgatgta ttcaaatgtt ttttctaaac gaatgccaag tcgtgtcgtg tattgcgtac
    12661 caaatcgaca aaactcttta ccaacacagc ttttaactga gcgtgttttc ttaccataag
    12721 ctgatgctga acgcatacct aggtcttccc atatatttgg taattcttct tttttaactc
    12781 catacaaacc aacacgttgt gaacctgtca ctttaactag tggcacatga tatttcttag
    12841 ccacttctcc tagacgaatc agttggtctg catctgtaac acccccacgc atttgaggta
    12901 taacagaaaa tgtaccatca ttttgaatat tcgcatggta acgttcgtta gcaaatcttg
    12961 attctctttc atcttcatga tcatgtggat aaaccatgtt taaataatag ttgattgctg
    13021 gtcgacattt tggacatcca cctttatttt taaagtttaa aacatgtcga acttctttag
    13081 atgtttttaa acctttcgct cttatttgcg ttactatttg atcgcgtgtc aaatcagtac
    13141 aaccacatat accagcaggt tttgcggcaa caaagtcatc tcctaaggtg tgctgcaata
    13201 tttgagcaat ttgcggttta catttaccac atgaattccc cgcttttgtt ttagccgtta
    13261 cttcttcaac tgttgtaaag ccattttccg taatcgcatt tactatagta cctttatcaa
    13321 caccattaca accacaaatt gtttcatcat cagccatatc agcaattgat agcgatgcct
    13381 cttctccacc tttagtaagc aatgatacaa gtgtgtaatc ttcagtggat tcaccttttt
    13441 tcatcatgtt ataaaagcgt gaaccatcat cgatatcacc atatagtact gcaccaacta
    13501 cattaccgtc ttttaaaaag atttttttat agttattatc aacactatta aatatttcaa
    13561 tacctttaat ttctgcattt tctacaattt gaccagcact atacaagtca cacccagaaa
    13621 cttttaatga cgtaaatgtt gttgatccct tgtatccgtt cgtttcttta tttgttaaat
    13681 gatcagctaa tactttacct tgttcatata gtggtgcaac gagtccataa actttgccgt
    13741 tatgttctgc acattcacca actgcatata cattgctatc acttgtttgc atcacatcat
    13801 tgacaacaat accacgatta acatctagac ctgattcttt ggctacttct gtgtatggtc
    13861 gtatacctac tgccataaca actaagtctg ccggaatctc gcgtccatca gccaatttaa
    13921 caccctcaac atcatcttct cctaagattt cagttgtgtt ggcttgcatt tcaaacttca
    13981 taccttgctt ttctagatct gctttaagca tatttccagc tttacggtct agttgcattt
    14041 ccatcaacca ttcagctaaa tgtaacaccg ttacttccat accttgatct aataaaccac
    14101 gtgcacactc taaacctagt aatcctccac caattacaat tgctttcttt ttagtcttag
    14161 caatgttcat catttgttca gtatcatcaa gcgttctcca accaattaca ctcggtaatg
    14221 tcgaacctgg aactggaatg acgaatgctt tagaacctgt cgcaaaaatc aatttatcgt
    14281 atgatacttc aataccattt gcagtagtaa ctgattgatt tgctctatct acttcaatta
    14341 caggatcatt tgtaattaac tcgataccat gttcctcata ccactcatat ggattcataa
    14401 ttgtttcttc aactgtcatt ttattttgta aaatatttga aagcatgatg cggttatagt
    14461 ttggataagg ttctttacct attaccgtaa tatcatataa atcgttggcg cgctctaata
    14521 tttcttcgat tgttcgaatg cccgccatac cgttaccaat cattactagt ttttgctttg
    14581 ccataaaata tgccccttta ctccataata tttatttcaa aaaaaggtat taatttttcg
    14641 ttagtgcttt tatattttca ttggaatcat taagctttct aatctatcgt taatgatttg
    14701 ctttaaaatt gggtcgaagt taattgaagg tgtgaagtgt atatctgtat taataaccat
    14761 gtcattcatt tgctgcttca ctttgttaac aagtcttccg tcatataaaa ataatggtac
    14821 gacaatcaat ttttgatacc gtttcgagat gctttctaaa tcatgtgtaa aactaatctc
    14881 tccatatagc gttctcgcat atgtcggctt gctaatttgc aaattttgag cgcatatttg
    14941 taactcttcg tgtgccttag taaactttcc attaatattg ccgtgtgcaa caaccataac
    15001 tccaacttgt tgttcgtcac ctgctaatgc gtcacaaata cgttgttcaa ttaatcgtct
    15061 cattaaagga tgtgtgccaa gtggctcgct tacttctacc tttatgtctg gataccgtcg
    15121 tttcatttca tgaacgatat tcggtatatc cttgagataa tgcattgcac taaagattag
    15181 caatggtaca attttaaaat ggtcaacccc actttgaatc aacgtcgtca ttaccgtctc
    15241 taaatcctga tgctcacttt ctaaaaacgc aatatcatag tgatgtatat catcttttac
    15301 taattcagaa ataaatgctt ctaacgcttg attctgtcgt ccgtgcctca tgccatgtgc
    15361 aacaatgata ttcccattca catttaccaa ccctttcaca cgtattgtat accaaatcat
    15421 tttgtttttg tgaaaagaat cacattataa tgtaaaatca gggaattccc tgatgcctgt
    15481 agtcatgcat attccttata cattttccct ttttgttaaa tcaaaaaaag cgaccgatat
    15541 atgaatccct actcaacatt tatttgagca agcattaata tatcggtcgc ttgtagtgta
    15601 tattattatc ttaaaatggt ggttggccta atattgtttc gtcaaagcgc tcgggtatca
    15661 atactttgcg catgatcaca cctaaatcgc catcatcatt ttcatgttcg ctgtatattt
    15721 cataacctct tttttcataa attttaagta accacggatg caatcttgca gatgtaccta
    15781 aagtaactgc cgctgacttt aacgtatctc gcaaaaatgc ttcttcaaca taagtaagta
    15841 attggctacc atagcctttc ccttcatact caggatttgt cgcaaaccac cagacaaaag
    15901 gataacccga aatacttttc acacttcccc aaggatatct aaccgtaatc gtagatataa
    15961 tttcatcatc aattgtcatg acaaatgtag tatttttatc tatattttct ttaacagcat
    16021 ctaaattagc attaaccgaa ggccaatcaa tacctagttc tcttagaggc gtaaatgctt
    16081 catgcatgag ttgttgcaat ttttctgcat cttgttcact tgcgagtcga atcatcgttt
    16141 ttgtcatatt aatccccact cttttttaaa tgatttaacc atattttatt tttaaaataa
    16201 atatccatca aagtgtatca ataaatttat cacatgtcag aaagtatgct tcatctgaat
    16261 acaccaatac tctcatgaaa cttattaaaa attactctct caacgtaaaa aaccattcaa
    16321 attcatgaat ggtttggaag aatgattcat tgttacgcta tttaatcact acatcttaat
    16381 tattgttgct ctaaacgatt acgcttacca tttaagaaag cataaacgag acctacaaaa
    16441 ataccgccac cgacaaagtt acctaagaaa gcaaaaacga tattttttaa aacgtgtaac
    16501 catgaaactg catcaaggtt aaagaatacc atacctgcat atagacctgc attgaacaca
    16561 acgtgctcat atcccatgta tacaaagacc acgacaccac aagctatgaa gaatgccttt
    16621 gttaagccgc ctttgaattg catagagatg aaaataccaa tattaataaa gaagttacag
    16681 aaaatacctt ttgtaaaaat attcaaccat gttgaatcaa cagtcttttt ctgaactaaa
    16741 gctgttaaag cttgtgtcat ttcaggtgtc atgacatgcg caaatttcat taagaaaaat
    16801 aacacaaacc cacctaaaat atttcctaag aaacaatata ataaaatcca agtcatcttc
    16861 ttaacagaaa cgactttata ataccagcct actgtaaagt acatgaagtt acttgttaat
    16921 aattcagagt tagttaatac aactaaaatc aaacctaaac taaacgcaat ggctcccatt
    16981 aaattgataa gtccatctac gtgagtactc gcaaactgcg tctttattcc gaacataaat
    17041 acagtaacga ttgagagtaa aaatccggcc atcattgctt taagcatata tcggccaggt
    17101 gtttgctgta ccattacttg cttcatttgt actgaagatg atattgaatc aacaattgcg
    17161 cctgttgaat atgtatcttc aactgttttc ttattttcta tcacagtact tcccccattt
    17221 actttgatca aataaaattt actttctaat tatattagta aatcaatgta attgatagat
    17281 taaatttcag aaaaatcttt ttattctctc gaaaatgtag ttttctttca taaaaacagc
    17341 gagataaatc gcgtgattgg ctaatatctt tatgatttaa gatattacga cgctcatttt
    17401 atctcgcttt atgtaaagta ttacttattc ttttttaaat ttgaaaagta atgtaaacca
    17461 tatgcaaata cactgtaacc aattgcagta cgtaaaataa accttataac tttattaggt
    17521 acacattttg acgttactga aatagaagat agtagtgttg tgaaaatata taatattaaa
    17581 actttgatat aacgttctac catcgaatca tccctcattt ttataatatt acaagattat
    17641 tataaaccga atgaaacaaa aaagttttta catattttca acaactttat ttcaataaag
    17701 catcaagttg atctaaagtt tgattcatac cttgttcaac acccatgttt atgacttgtt
    17761 gagcggcttc ttttgttgga aataccgatg ttgatgtcac tgtcgttttc gttgtattgc
    17821 tcttggaaaa tgacaaagta attttcatgc ttggcatttt tgtatctttt gcaccttgag
    17881 gtgtcgcaaa tgagtcaata tattcaatta aatatggacg cttaaccgtt ttatattctg
    17941 ctaatgtata gctggtcatt gtaggtgttt taatcgcata aaatgcatca ccgcctgaaa
    18001 cagcattaaa gcgaaacact ttggtactag cgtcttttgg atgaaaccat ttttcaaata
    18061 atgcttttgt cgtataggca tcaaacacct tttcaatcgg tgcttctatc gttcttgaaa
    18121 aaataatttt attatcttca actttaatcg tcatcttccc actccctatc ttttatattt
    18181 actcatctta acatgcattg agtaacatat gttaccaaat catttgttat aaactatagt
    18241 aatattaatc attccctctg ttgaaattga taactacgaa ataaaaaaca cactctattc
    18301 agctattaac agttgagtag agtgtgcaaa cctgttgaat taatgcgcta acatttcttc
    18361 tttgatttgg tctttattta attttgaagg ataatatgtt ggccagttat ccatttcttt
    18421 taataacgcg tcattatcat tacccataaa gatatggaag tgttctgcct ttttaggtgc
    18481 aatgttgtga tcgctaaatt ggatgaattt cggtaagtct ttatcattac catcgaccaa
    18541 tttaaacata aatctcacgc cacggttacc tttaggatat ttcaatgttt tcttaccatt
    18601 gtattcatat ttaccagtgt gtttcttacc atctttagta aatgtaattt catttccttt
    18661 tatatcaatg ttagtaatat cagttttata tcctttgtca taataagctt ttaaatcttt
    18721 agcagatttc ttcggatcat tttcagcttt atgttccatc acttcatcaa gcgtaccgtc
    18781 ttttaagtaa gggtaaacag attgccattc accagcataa tcgcttaatt cacggtcttt
    18841 aacttgctca tctttaaaat acccatcaga aattgctttg tcatgtttac tttcagcttt
    18901 gtcgtctttc actttaacac cactgtctaa agctttaccg atattttcaa tattagattt
    18961 cattaatgat tgatacgtaa tattatcttt tttctgttgt tctttattta aagactccat
    19021 gttgtagaat tttaaaggct tcgcatctgt ttctttacga attgtttcag tcactttatt
    19081 cgcaacatta tcttcgtaaa gaatatattt agcattgcta tctctaattt ctttaacaat
    19141 tttagttaat tctttttgtg atggatcttc agcattcatg ttttgaatac ctttttgaac
    19201 aaaaccataa caatcagcta agtatccaat tgattcatgt gaaatgaata ctgcattacc
    19261 ttgcttatct tttgtaactt gcttcatatc gttatcaatt ttcttaagat cgtcgtttaa
    19321 ttttttgtag tttttctcat agtcatcttt atgttttgga tcttttttca ctaattcatc
    19381 tttaatttct ttagcgaaag tttggttaat tttaggatct aaccatacgt gtggatcata
    19441 tccaccatga tgatgatgat gttcttcttt ctcatgatca tgtccctcat gttcatgctc
    19501 ttcaccatgc tcgtgttgat cagttaaaag ctttgcttta tctaatttat cctctaaaga
    19561 cagtttttta tctttatctt tgatagtaga tgcaactttc ttagcaaccg gatctaaatt
    19621 atcccctgta tacataaaca agtctgactt gcttgcactt aatatatctt tttgtgttgg
    19681 ttcatagcta tgtaaatctg tccctgctgg atagattgat gataccttca cgtgttttcc
    19741 accaatttgc tctgcaaatg attgcaatgg ataaactgtc gttttaattg ttaccttgcc
    19801 atctttatct tttccatcat cattcccaca tgcggctaat gataaagtta cggctggtac
    19861 aagaagtaac atacctaatt tctttttcat agtcaccctc ctaaatagta atctttacgt
    19921 tttaaaatta tatactcatg ctacttaaaa tacaagcact gtttttataa ataatataaa
    19981 gtcgaatcat actttaggta tttaccactt ttcgttttat ctaatacatg actattgtac
    20041 aaatatgata cagaaaactt tatagtattt acgaacgtca taatataaga aaaaaagaca
    20101 atctctatca tattgctaat agaaattgcc ttttcatatt gtcctaaaat aacttaccct
    20161 atttttatag tgtgactttt agatttaata atggtattta catgatgcta tttttatatg
    20221 attgtcatct actgtataaa ccaatctatg ttcgtgattg atacgtctac tataataccc
    20281 agtcagattc gattttaact tttcaggctt acctatacct tccaatgcac catttctgtc
    20341 aatactttta agtaactcat taatcttctt caccattttt ttatcgttct gctgaaaata
    20401 cttataatct tcaaaggctt gaggcgaaaa cgtaatattt aacctagcca ttatacatct
    20461 atatctttcg ttatagtttt cccacgttct agatctgcaa ttgattgagc taagtgttca
    20521 gcattatttg ggttctgttg gaggtaaagt gtttccatca tggagttata atctgattct
    20581 gacatgatta ctacgttttt atcatcagta gatgttacag ttaccataac actatcatca
    20641 tttacttttg tcataagtgc ctttaaattc tgtcgagcgt atgaataatt tttaataatc
    20701 atatttatcg cctcctttta acacacctca attgtacaaa tatctgtaca ataaaacaag
    20761 cgacacagaa aaaaactact aatcaagatt tcgcacgggg aagaatcaat acctatcctg
    20821 tactgattcc ctactaaaca atgtttacat gataatttcc acctatttag acttcataca
    20881 ttaaaaagac aatctctatc atatcgctaa tagaaattgc cttttcgtat tgtcctaata
    20941 aattggtatc acttactgat ctcaacaatt aagactattt gattttatct tttaataact
    21001 tctcataact ttcataatca tatggatctt caactttctc gccattaata aatgcagttg
    21061 gtgtcgtttt tatatgatta tctttcgcta tttttttatc tttctctgca gctttccaag
    21121 acttgctatc ttttgtcttg taatctttta taattttatt ttccgtctct ttatctaaat
    21181 gcagttgttt aatatgttta tctaatagtt cttttgttaa ccattcttta ttttcatctt
    21241 gctgggcagc aaataattgc ttttgaaaat ctaaaaatga tttaggtgca tacatcaata
    21301 ctgcatgact cgcacgcgaa ccaacaattg agtctttacc taagaaagct aaattgacaa
    21361 attggtattc cactttgtga ttatctatat aatttttacg caactttggc atgacttttt
    21421 catctaattc tttacaataa ggacatttat agtcgccata tacgacaact aatggtttgc
    21481 cgtttttcga agatgtcgtt gctgattctt ttttaccgca agctgttaaa attaacatgc
    21541 tcactataaa taatgttagt aattttttag tcataataac tcctattcat atctagttcg
    21601 tcatattttc ttcatgataa tccgcatcat gttgttgata atatcgtctc gaatcaaaat
    21661 cttgcttata tgatacacgt ttatcactaa aatcatatgc ataataatgc acttcatcat
    21721 tactcaaagg tttataagct aaaatgataa ttttatcctt atcatagaca taaatattag
    21781 catctttgcg ttcaaaatga tcaacacgat caccgttgcc ttttttggca agttgttctt
    21841 gtttttgatt ttgaatttta actgcttcat ctatttcttt cacatattta tcattaccac
    21901 atcctgatat aaagactagt aataataata ccgtcgccac aaatcgcttc aatgctccca
    21961 ctcctactat caacttccaa ctaactttca atataattaa tactatacac cgtcaaacat
    22021 taatgcgcta caactactac caatgcattt aatataaata tccaaagaaa taacagttat
    22081 gtatagtcaa agaaaaatat atttcatttt ctatgtgtat ttgtaatcag tttttgataa
    22141 aataaaaaaa tagcattatt aataaattca aaaattatag ttcaattcag agagggatgg
    22201 gataaaatga actttgtaac gttgacttcg gatgagttca atgcgtttac aacaaagcat
    22261 ttttcacatt acacacaatc agctattcat tacaatcata gagttgattt aaaaggcgat
    22321 gtgcatcttg taggggttaa agatgacaat ggtcaagtga ttgcaggatg cttattgaca
    22381 gaagcacgca cacttaaatt tttcaaatat ttttatacac atcgcgggcc agtgatggat
    22441 tatacaaatc aatcattagt agcatttttc tttaaagcat taacgtcata tttaaagaaa
    22501 cacaattgtt tatatgtcct tgtagatcca tatttaattg aaaatttacg caatgcagac
    22561 ggtgaaattg ttaaatctta tgataaccga gcatttgtta gaacaatgga taaattaggt
    22621 tataaacacc aaggtttccc tgtaggttat gattcaatga gccaaatccg ttggctgtca
    22681 gtgttagatt taaaagataa gactgaagac caacttttaa aagaaatgga ttatcaaacg
    22741 agacgtaata ttaaaaaaac atatgatatt ggtgtcaaaa ctaaaacgtt aacgattgat
    22801 gaaacgcaaa cttttttcga cttattccat atggctgagg aaaagcacgg tttcaaattc
    22861 cgtgagttac catactttga agaaatgcaa aagttatacg atgaccacgc catgttaaag
    22921 ttggcgtata ttgatttaaa cgagtattta aaaacgttac aattaaagca acaacaatta
    22981 acagctgaac tctcaggtgt tgaagaagca ttggaagaaa gtcctaattc aaagaaaaat
    23041 aaaacgaaac gcacacagct agaacaacag ctaaatagca ataagcgtaa aatcgacaat
    23101 acaatagaac aaattgaaca agatggtgca gtcttaaact tagcttctgc tttatttatc
    23161 tacaatgagc atgaagttta ttacttatct agtggttcaa accctaaata taatgcttat
    23221 atgggtgcct accatttgca gtgggaaatg attaagtttg ctaaagcgca tcatattgat
    23281 cgctataatt tttatggtat taccggtgac ttttcagagt catcagaaga ctatggcgtc
    23341 caacaattca aaaaaggttt taacgcacat gtagaagagt atatcggtga ctttattaag
    23401 cctatcaaac cattactata taaggtacaa acgtacttaa atcataaacg tcgttaaaat
    23461 atcgctccaa gattatgaca acataacaaa gaaccacgca tcatgtatat ggcttcatcg
    23521 ccatactaaa acatgatacg tggttctatt tgattattct tcatttataa catttaagaa
    23581 acgtcttaat tcttctgttt tcggacgatt gaacatctct tctggagccc cttgttctcc
    23641 gatcatgcct tcatgaataa atacaatgtt attagatact tctttagcaa aacgcatttc
    23701 atgtgtcaca atgaccattg tcatgccttc attagccaaa tctttaataa cctttaaaac
    23761 atcattcaca agttcaggat caagtgcaga tgttggttca tcaaacaaca tcactttagg
    23821 gttcattgct agtgctcttg caatagcaac acgttgttgt tgaccacctg ataatgcatg
    23881 tggacgttga tctttgacat gtgttaaacc aactttctca agtaactcaa gtgatttccc
    23941 acgtgcctca tcctttttca actttttaac tgtgataaga ccttccatta cattttctaa
    24001 tgccgtctta tgcggaaaaa ggttataact ttgaaatacc ataccagact gtttacgaac
    24061 ttctatttgt gattttttat ctttagatgt atatgttttg ccgttaacat aaactgtacc
    24121 ttcagttgga atttctaatg cattaatcat acgtaacaat gttgttttac ctgaaccaga
    24181 tcgaccgatt aaggttacaa cctcaccttg ttcaacagat aaatcaatac ctttgatgac
    24241 ttcaacatca ttaaatgatt tatggatatt gttcaattga atcatgagcg ataccctctt
    24301 tcaatataag attcatagat accttggata attgaaataa taaagcatac cacccagtac
    24361 attagcgcaa ctaacacata aattgttaaa tattcatatg ttgtagacgc aacttcttga
    24421 gcttttctaa acatttcagc cactaaaata aatcctaata atgatgtatc tttgattaaa
    24481 cttaaaaatg tattacctag tgcaggaatc gacactcgaa ttgcctgcgg taaaatgata
    24541 cgttgtatcg tttgaccata cgtcataccg atggaatatg cagcttctgt ttgtcctttc
    24601 ggtatagaaa taataccgcc acgaataatt tccgaagcat acgcacctac atttaatgat
    24661 aaaccaataa tagcagccac tacaggtgcc aatgtccatt ggttgtcagc gtcatttgtt
    24721 aataatctac ctaattctgg aataccataa aatatgataa atagttgtac tatcattggt
    24781 gttcctcgaa taatagatac atagacacgt gaaatacttc ttaaaatttt actcgttgaa
    24841 attcgcatta atgctgtaaa taatgcaata atcaatccta aaacaaatgt aactaatgta
    24901 ataggaattg aatatttgac caaaccttct agcataggtc cgaaagcttg ttttgcagca
    24961 tctagcgcat gttgttgttc gctatttaga tttagaaaca tcttgaccaa accatttctt
    25021 acctatttta gctaattcac cgttttcctc gatttttttc aagccatcat tgaatttttg
    25081 aactgtttca tcatctgctt ttttagaaaa tgcaaatgca gatctacttt gttcagcatt
    25141 acctttgatt gctttgatct tagcattagg tttttgtttt ttataatcca agtatgacag
    25201 actatcatta aatgtaccat caacacgctt agacaataat aaatccattg attggttaaa
    25261 gccatcaact tttgtaatat cagcaccttt atccttagct aatttaccat aattagatgt
    25321 gaatgtttgt gctaacttct tacctttaac atcatcaaaa tctttaatat ctttttcatt
    25381 ttcacgaata actaaaacag cacttgagaa tgtgtaaggc ttagaaaatt tatatttctt
    25441 ttctctatca ggattaatac ctacttggtt cgcgattaca tcaaaacgcc ctgcgtctaa
    25501 acctgcaaac atagaatccc aagaagtttc attaaattta agttttaaac cttcttcttt
    25561 agccactgct ttaataacat caatatcgta accagttaat ttaccatctt tatcgtggaa
    25621 actaaatggt gcataagtcc cctcagtccc aacattaatt gtcttgctat ctttactagc
    25681 ttccttgtct ttcgacgaat tgtttccgca tgcagccaat atgaaaacga aagctatcat
    25741 cacaaataaa agtcttttca tagaatatat ctccttattc ttattattct aatcggaata
    25801 aaaatatttt aatctgaaat gccggataag tcaatatgtt gaacttttgc taaaaagctt
    25861 tatatagcta gcaacttata tttattgacg agaatcaact tcttttttct taccttgtaa
    25921 cataaatgaa caaaagaaac taacaactgt aatcaataat gcaaataaaa atccagcgtg
    25981 ataaccatgc aacatcgtat caactttaat ttgattcatc atatcaattt tgttcatgcc
    26041 attatagtct gacatagttg gagaaaagtt tttgcttgca tgggataaaa tagtaattaa
    26101 tgcagctgtt ccgatagaac cagcaatttg ttgtactgtg tttgtcatag atgaaccatg
    26161 tgcattcatt tctggcttta attgattcat cgtatgggtc attactggaa ttaatcctaa
    26221 cgcgatacca accattcgaa tcgcataaac tgttgccaac atgattgttg atgtttgttc
    26281 atccattaca acaaaataag ccgtagtaat aacaacaatc gccataccta ctaaagcaag
    26341 atttctagga ccaaactttt catataaagc acccgaagtc atcgacataa atgccataac
    26401 aattgctcct ggtaataaag ttaatccaga atctaaagct gaacgatgca ataaattttg
    26461 cacaaagata ggtaaaatcg tttccgaacc aatcatcgat accattgtga cacccataat
    26521 aatgatacca actgtaaatt gtttattttt aaatacagca aaatttaata atggtgttga
    26581 tagccgatat tgacgtaata caaataatgc gactaaaaca acgcctccaa tcatagatac
    26641 taatacgata ggattatcaa aacctttttc tgaaattgaa ctcgttccat acaataaccc
    26701 gccgaaacct agtacagaaa aaatgacaga aataaaatca ataggtactt taattgtagt
    26761 tccaacattt tttatcgttt taaatccaat taaaaatgtc acagctgcaa ttggagcgac
    26821 aacatggaaa agcgatctcc agttaaaata ttcaacaagg taacctgaaa gagtaggtcc
    26881 aatagctggt gctaaaccaa ttaccaaacc aaatatcccc atatatttac cgcgttcatg
    26941 tggttggaaa acatccaaaa tcgttgtcat catcaatggc atcataatac ctgcgcctaa
    27001 cgcctgaata attctagcaa ctaataatgt tgcaaagtgc ggactcaagc cgccgagtaa
    27061 tgtacctaag aaaaagatag aaatacccac taaaaacact tgtcttgttg tataacgttg
    27121 tataacaagc gcggacaaag gaataacaat cccattagtc aataaaaaag ctgttgtcag
    27181 ccattgaact tgtgtatatg aaatttcaaa atctttcata atacttggta aagccgtcac
    27241 taatagtgtt tcatttaata atccaaaaaa tccacccatc agcatagtaa taatcatcgt
    27301 taatttttgt ttcttcgaca tttaaagctt ccccctcacc atctaaaaaa cgacacacat
    27361 ttttagatat ccggtaatta tacccatttt ccaaaaaatt agcacgactt tattacaatt
    27421 tctttaaaac gtaatcatta ctatttacat atgagtagtt atcggtcata atatatttac
    27481 aaggagggat agagatgaaa ttagatttac agactgctcg tcgtaattta aatagcccta
    27541 acattaaaac aagaaagcgt gctttaaaga ttattaagca acataaaaga gcgaaataat
    27601 tgttttaatt tgactatatt gtatgtctaa ctatattttc gagtatccct agctcgttaa
    27661 tataaactct atacttaact atttccttac attacttcac atatttaaga gctgcatata
    27721 cactcactcc ttgtgtacat gcagctcttt ttttataagt agtatttatc tataacttct
    27781 aaatcatccg ttaattcata aacaagcggt gcacctgttt taatttcata attaatgata
    27841 tcttcatctg acacatcttc aagatattta atcaatgcgc gaattgaatt tccgtgtgca
    27901 gaaactaata ccgtttgacc atctagcaaa tattgtgaaa tatgatctgt ccaaaatggt
    27961 atcactcgaa ctaaagtatc tttcagactt tcagaataag gcatcatacg tttatctaaa
    28021 tgattatatc gacgatcagc taagtaagct tcacgttgtt cttcggtttc agcaggtggt
    28081 ttcacatcat aagaacgacg ccaaatatgt acttgttctt ctccaaattc ttttctagca
    28141 tcatctttat ttaagccttg caatccacca tagtggcgtt catttaaacg ccagctttta
    28201 tatacaggaa tccattgttg tttagattca gttaaaatat aatgcgttgt atctaaagca
    28261 cgtgttaata acgatgtaaa agctacatcg atggcaatgt tattttctct tactttttca
    28321 cctgctctag tcgcttcatt aataccttgt tcagataaat taacatcttc ccatccagta
    28381 aataagtttt tagcattcca ctcgctttgt ccatgacgac ataaaattaa ttttggcata
    28441 acttataacc tctttccttt aatctttagg acgttatttc agggatattt taagatgatt
    28501 taatcttagt aattttcttt tcaatatatt ttaaaattca atgattaaat caattatcac
    28561 tagacattaa atacataaat cctattcccc attttcattt tttaattcat aaatgaatca
    28621 ataaccacct aataacaaat catattatac acctttgttc gctatttttc taaggtttaa
    28681 aaaatatttt taggtaaacc taaaaataga tgtaataaaa acgcctcctc agatatttat
    28741 atatctatga agacgtttaa atacattata gatggtctgg ttctgggtga acgtatactg
    28801 aggaaatacc ttttttgtgc aaatgatgtt cgacattgtc acaaatttga tgcgcttcta
    28861 ctaaggaaag gttagcatct acaacaattg tgacatcaat aaacacacta cttccatggt
    28921 aacgcccttt aatactttta acttcttgta cttcatcaac ttctaaaata tcattgcgat
    28981 acgcttctaa ttcagtttca ttgaaaccat cactcaacat aaaaattgct tctttaaaaa
    29041 taccaaaacc agtataaacg attagtaagc ctagtaatgt tgctaaaata atatcgacaa
    29101 ttgggaaacc gatttgcgta aaaattaatc ctatcgctgt tccaatgctg actaaactat
    29161 ccgataaatt atctttggca gccgaattta aagaactact tttcgttctt ttcgctagtc
    29221 tttgattgac tgcaaataca atcaacatta caagaccact gattaagctg acgataattg
    29281 ttattgcgtt aggtacaacg tcatcttctt tgaacaaacg aggtgcattt tgaataacta
    29341 cttggatacc tacaaacata atgacaaatg acaccaataa tgaagaaata ttttcagact
    29401 tcaaatggcc ataaggatga tttcgatcgg caggtttaat tgaaattttc aatccaataa
    29461 taacagctaa agaaacgata atatctgtca tattgtttaa tgcatcggct cttacagctg
    29521 cagagttaaa gacaaaaccc gtgacatact taacaataga taagattata tatacaatta
    29581 aactcaaata agcaccgcgt tgcgccaatt taagattttc attatgagac atgcgttgaa
    29641 ccaccttgaa ttagtatagt aacaatatta tgaatgattc attttaattt tacaacgttt
    29701 ttaattttta taaattttta taaaattaaa ctaatttatt cattcgcaac ccctaaaaat
    29761 aatttttagc ctttctgcga attttatgag ctagaaaggc gcccaactct ccctgtttgt
    29821 taactttcgc ctcgaaagtt tctatgttgg gaccctatgc attagtagcg aagctatcta
    29881 atgtgttcct ttattttctg cgaattttat gagctagaaa ggcttatgca gttgacgttt
    29941 tacgtccaac tgcgttcctc cgtcttcttc aaatttattt gttagaaagg cacccaactc
    30001 tccctgtttg ttaactttcg cctcgaaagt ttctatgtta gaaccctatg catgagttgc
    30061 gaagctatct aatgtcgtga actaattata tagaagaaaa agtgcatcaa tgacaaatta
    30121 aatgagattt ctactctacc aaactctctt cgaaagacaa ttttctcctc tatttattag
    30181 caaactattg catttctcca tatagtactt ccttacttaa aatacgctga atgttctgaa
    30241 ttaaacgctt ttttacatag ttaacactag ttaatctatt agttaacatt agttaataat
    30301 tagttaattt ccatttgtat tctcatgtga taaattctaa aagcatacaa taaatttaat
    30361 atgtaaaaag aaagggaata cacatgaaaa ataaatatat ctcgaagttg ctagttgggg
    30421 cagcaacaat tacgttagct acaatgattt caaatgggga agcaaaagcg agtgaaaaca
    30481 cgcaacaaac ttcaactaag caccaaacaa ctcaaaacaa ctacgtaaca gatcaacaaa
    30541 aagcttttta tcaagtatta catctaaaag gtatcacaga agaacaacgt aaccaataca
    30601 tcaaaacatt acgcgaacac ccagaacgtg cacaagaagt attctctgaa tcacttaaag
    30661 acagcaagaa cccagaccga cgtgttgcac aacaaaacgc tttttacaat gttcttaaaa
    30721 atgataactt aactgaacaa gaaaaaaata attacattgc acaaattaaa gaaaaccctg
    30781 atagaagcca acaagtttgg gtagaatcag tacaatcttc taaagctaaa gaacgtcaaa
    30841 atattgaaaa tgcggataaa gcaattaaag atttccaaga taacaaagca ccacacgata
    30901 aatcagcagc atatgaagct aactcaaaat tacctaaaga tttacgtgat aaaaacaacc
    30961 gctttgtaga aaaagtttca attgaaaaag caatcgttcg tcatgatgag cgtgtgaaat
    31021 cagcaaatga tgcaatctca aaattaaatg aaaaagattc aattgaaaac agacgtttag
    31081 cacaacgtga agttaacaaa gcacctatgg atgtaaaaga gcatttacag aaacaattag
    31141 acgcattagt tgctcaaaaa gatgctgaaa agaaagtggc gccaaaagtt gaggctcctc
    31201 aaattcaatc accacaaatt gaaaaaccta aagtagaatc accaaaagtt gaagtccctc
    31261 aaattcaatc accaaaagtt gaggttcctc aatctaaatt attaggttac taccaatcat
    31321 taaaagattc atttaactat ggttacaagt atttaacaga tacttataaa agctataaag
    31381 aaaaatatga tacagcaaag tactactata atacgtacta taaatacaaa ggtgcgattg
    31441 atcaaacagt attaacagta ctaggtagtg gttctaaatc ttacatccaa ccattgaaag
    31501 ttgatgataa aaacggctac ttagctaaat catatgcaca agtaagaaac tatgtaactg
    31561 agtcaatcaa tactggtaaa gtattatata ctttctacca aaacccaaca ttagtaaaaa
    31621 cagctattaa agctcaagaa actgcatcat caatcaaaaa tacattaagt aatttattat
    31681 cattctggaa ataatcaatc aaaaatatct tctctagttt tacatcattt tttaaataat
    31741 tttcgtaaca aaccgtgatt aaaaagaacc gttgattctc aatcgaatct acggttcttt
    31801 tttcattttc catcaattaa atgcttcttc gctatttgtc agcccacttt tttacctgca
    31861 acttgttaaa taatccttac atcgttaacg aatagttcat catttagttg aatcagctca
    31921 actttattaa cttcatattt tcacaaacta ttgcgcaatc cattcctttt ccactacaag
    31981 caccataatt aaacaacaat tcaataaaat aagacttgca aagcatagtt atgtagctat
    32041 ataaacgcct gcgaccaata aatcttttaa acataacata atgcaaaaac atcatttaac
    32101 aatgctaaaa atgtctcttc aatacatgtt gatagtaatt aacttttaac gaacagttaa
    32161 ttcgaaaacg cttacaaatg gattattata tatatgaact taaaattaaa tagaaagaaa
    32221 gtgatttcta tgattaaaaa taaaatatta acagcaactt tagcagttgg tttaatagcc
    32281 cctttagcca atccatttat agaaatttct aaagcagaaa ataagataga agatatcggc
    32341 caaggtgcag aaatcatcaa aagaacacaa gacattacta gcaaacgatt agctataact
    32401 caaaacattc aatttgattt tgtaaaagat aaaaaatata acaaagatgc cctagttgtt
    32461 aagatgcaag gcttcattag ctctagaaca acatattcag acttaaaaaa atatccatat
    32521 attaaaagaa tgatatggcc atttcaatat aatatcagtt tgaaaacgaa agactctaat
    32581 gttgatttaa ttaattatct tcctaaaaat aaaattgatt cagcagatgt tagtcagaaa
    32641 ttaggctata atatcggcgg aaacttccaa tcagcgccat caatcggagg cagtggctca
    32701 ttcaactact ctaaaacaat tagttataat caaaaaaact atgttactga agtagaaagt
    32761 cagaactcta aaggtgttaa atggggagtg aaagcaaatt cattcgttac accgaatggt
    32821 caagtatctg catatgatca atacttattt gcacaagacc caactggtcc agcagcacga
    32881 gactatttcg tcccagataa tcaactacct cctttaattc aaagtggctt taatccatca
    32941 tttattacaa cattgtcaca cgaaagaggt aaaggtgata aaagcgagtt tgaaatcact
    33001 tacggcagaa acatggatgc tacatatgct taagtgacaa gacatcgttt agccgttgat
    33061 agaaaacatg atgcttttaa aaaccgaaac gttacagtta aatatgaagt gaactggaaa
    33121 acacatgaag taaaaattaa aagcatcaca cctaagtaaa cagttcaatc atcttaaaaa
    33181 atcctgggac acttcatact tgtctcagga ttttttaaca aattgaatca gccttataac
    33241 attaaattat tttatcgtac attaaattta ataataacaa ctgattttta taagaataaa
    33301 gtatcgaacc atagtagata cacaaataat acaaatgaaa caatttaact tgaaagctta
    33361 aataaatatt atcaagttaa taaacaatta atttttagat ggattcatca aaaatcgtaa
    33421 aaagcacaat ttgtatttta caaacattaa ttaaaaaaga aagcaagaca ttcgtgcaat
    33481 cggttacctt aaattgttta caactgtcaa caataccaag gttttattaa ctatatttct
    33541 cacaaaatta gcttttagca ttccaaacaa aaaaggttaa atcgaacgga attatggcat
    33601 ttttaactta attgtaaaaa aagttgataa tggtcaattg ttaatgaaca gttaattata
    33661 ataacgccca aaatatatta ttatttaatt aagttaaata aaattataga aataaagt
//
LOCUS       DAKTLH010000014        33222 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.014, whole genome shotgun sequence.
ACCESSION   DAKTLH010000014 DAKTLH010000000
VERSION     DAKTLH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 33222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33222
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.014"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            228..530
                     /gene="gatC"
                     /locus_tag="O0394_000604"
     CDS             228..530
                     /gene="gatC"
                     /locus_tag="O0394_000604"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCY0498705.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            532..1989
                     /gene="gatA"
                     /locus_tag="O0394_000605"
     CDS             532..1989
                     /gene="gatA"
                     /locus_tag="O0394_000605"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCY0498706.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            2002..3429
                     /gene="gatB"
                     /locus_tag="O0394_000606"
     CDS             2002..3429
                     /gene="gatB"
                     /locus_tag="O0394_000606"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCY0498707.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
     gene            3469..3615
                     /locus_tag="O0394_000607"
     CDS             3469..3615
                     /locus_tag="O0394_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498708.1"
                     /translation="MIADLDAIEGLFISIEVKLLLTLFIVHVNGKNINFINALALIIL
                     RQTV"
     gene            4140..5087
                     /locus_tag="O0394_000608"
     CDS             4140..5087
                     /locus_tag="O0394_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCY0498709.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
     gene            5168..6529
                     /gene="rlmD"
                     /locus_tag="O0394_000609"
     CDS             5168..6529
                     /gene="rlmD"
                     /locus_tag="O0394_000609"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCY0498710.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPLAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            6699..7229
                     /locus_tag="O0394_000610"
     CDS             6699..7229
                     /locus_tag="O0394_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCY0498711.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            7476..8546
                     /gene="dinB"
                     /locus_tag="O0394_000611"
     CDS             7476..8546
                     /gene="dinB"
                     /locus_tag="O0394_000611"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCY0498712.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(8614..9168)
                     /locus_tag="O0394_000612"
     CDS             complement(8614..9168)
                     /locus_tag="O0394_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HCY0498713.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKIHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            9383..9475
                     /locus_tag="O0394_000613"
     CDS             9383..9475
                     /locus_tag="O0394_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498714.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            complement(9557..10057)
                     /gene="ftnA"
                     /locus_tag="O0394_000614"
     CDS             complement(9557..10057)
                     /gene="ftnA"
                     /locus_tag="O0394_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HCY0498715.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
     gene            10349..11662
                     /gene="murT"
                     /locus_tag="O0394_000615"
     CDS             10349..11662
                     /gene="murT"
                     /locus_tag="O0394_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HCY0498716.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            11664..12395
                     /locus_tag="O0394_000616"
     CDS             11664..12395
                     /locus_tag="O0394_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCY0498717.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
     gene            12481..12590
                     /locus_tag="O0394_000617"
                     /pseudo
     CDS             12481..12590
                     /locus_tag="O0394_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12619..13032
                     /locus_tag="O0394_000618"
     CDS             12619..13032
                     /locus_tag="O0394_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498718.1"
                     /translation="MKPTKVILKDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMDCDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINVAKSDFTKFINELASLH"
     gene            complement(13522..14508)
                     /locus_tag="O0394_000619"
     CDS             complement(13522..14508)
                     /locus_tag="O0394_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCY0498719.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            15036..15794
                     /gene="map"
                     /locus_tag="O0394_000620"
     CDS             15036..15794
                     /gene="map"
                     /locus_tag="O0394_000620"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCY0498720.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            16011..16397
                     /locus_tag="O0394_000621"
     CDS             16011..16397
                     /locus_tag="O0394_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498721.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            16412..17113
                     /locus_tag="O0394_000622"
     CDS             16412..17113
                     /locus_tag="O0394_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCY0498722.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            17110..18153
                     /locus_tag="O0394_000623"
     CDS             17110..18153
                     /locus_tag="O0394_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0498723.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            18143..18772
                     /gene="vraR"
                     /locus_tag="O0394_000624"
     CDS             18143..18772
                     /gene="vraR"
                     /locus_tag="O0394_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HCY0498724.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMDDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(18888..20105)
                     /locus_tag="O0394_000625"
     CDS             complement(18888..20105)
                     /locus_tag="O0394_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCY0498725.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIIFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
     gene            complement(20383..20658)
                     /locus_tag="O0394_000626"
     CDS             complement(20383..20658)
                     /locus_tag="O0394_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCY0498726.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            complement(20665..21129)
                     /locus_tag="O0394_000627"
     CDS             complement(20665..21129)
                     /locus_tag="O0394_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCY0498727.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            21276..21476
                     /locus_tag="O0394_000628"
     CDS             21276..21476
                     /locus_tag="O0394_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HCY0498728.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHE"
     gene            21479..22735
                     /locus_tag="O0394_000629"
     CDS             21479..22735
                     /locus_tag="O0394_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCY0498729.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAGKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            22825..23355
                     /locus_tag="O0394_000630"
     CDS             22825..23355
                     /locus_tag="O0394_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCY0498730.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(23615..24766)
                     /gene="yfkAB"
                     /locus_tag="O0394_000631"
     CDS             complement(23615..24766)
                     /gene="yfkAB"
                     /locus_tag="O0394_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HCY0498731.1"
                     /translation="MNSVKLKQPVSIYNDPWEAYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRNAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            24990..25151
                     /locus_tag="O0394_000632"
     CDS             24990..25151
                     /locus_tag="O0394_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498732.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            25282..25797
                     /locus_tag="O0394_000633"
     CDS             25282..25797
                     /locus_tag="O0394_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCY0498733.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            26138..26947
                     /gene="sgtB"
                     /locus_tag="O0394_000634"
     CDS             26138..26947
                     /gene="sgtB"
                     /locus_tag="O0394_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HCY0498734.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            27208..28026
                     /gene="recX"
                     /locus_tag="O0394_000635"
     CDS             27208..28026
                     /gene="recX"
                     /locus_tag="O0394_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCY0498735.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEIFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            28004..28318
                     /locus_tag="O0394_000636"
     CDS             28004..28318
                     /locus_tag="O0394_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HCY0498736.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            28333..29850
                     /locus_tag="O0394_000637"
     CDS             28333..29850
                     /locus_tag="O0394_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0498737.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHDQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            29859..30695
                     /locus_tag="O0394_000638"
     CDS             29859..30695
                     /locus_tag="O0394_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498738.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIVLIVFAVVMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGVCVLCFLLLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
     gene            complement(30956..31933)
                     /locus_tag="O0394_000639"
     CDS             complement(30956..31933)
                     /locus_tag="O0394_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCY0498739.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            32085..33122
                     /gene="mutY"
                     /locus_tag="O0394_000640"
     CDS             32085..33122
                     /gene="mutY"
                     /locus_tag="O0394_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCY0498740.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
BASE COUNT        11996 a         4701 c         5901 g        10624 t
ORIGIN      
        1 tttttggata tgagcaaatt tttataatta aaattgcata atcataatat tgaattgggt
       61 tgtctaatat taaaagagtg ttgttttcat aaaaaatgat ttgataaacg aatgataatg
      121 tttatacagt ttatatgaaa ttaaagtagc acctcataaa tacttagatt tttaattgga
      181 aatttgatac aatttagtga tgaatgactt aaaggaggct tttattaatg acaaaagtaa
      241 cacgtgaaga agttgagcat atcgcgaatc ttgcaagact tcaaatttct cctgaagaaa
      301 cggaagaaat ggccaacaca ttagaaagca ttttagattt tgcaaaacaa aatgatagcg
      361 ctgatacaga aggcgttgaa cctacatatc acgttttaga tttacaaaac gttttacgtg
      421 aagataaagc aattaaaggt attccacaag aattagcttt gaaaaatgcc aaagaaacag
      481 aagatggaca atttaaagtg cctacaatca tgaatgagga ggacgcgtaa gatgagcatt
      541 cgctacgaat cggttgagaa tttattaact ttaataaaag acaaaaaaat caaaccatct
      601 gatgttgtta aagatatata tgatgcaatt gaagagactg atccaacaat taagtctttt
      661 ctagcgctgg ataaagaaaa tgcaatcaaa aaagcgcaag aattggatga attacaagca
      721 aaagatcaaa tggatggcaa attatttggt attccaatgg gtataaaaga taacattatt
      781 acaaacggat tagaaacaac atgtgcaagt aaaatgttag aaggttttgt gccaatttac
      841 gaatctactg taatggaaaa actacataat gaaaatgccg ttttaatcgg taaattaaat
      901 atggatgagt ttgcaatggg tggttcaaca gaaacatctt atttcaaaaa aacagttaac
      961 ccatttgacc ataaagcagt gccaggtggt tcatcaggtg gatctgcagc agcagttgca
     1021 gctggcttag taccatttag cttaggttca gacacaggtg gttcaattag acaaccggct
     1081 gcatattgtg gcgttgtcgg tatgaaacca acatacggtc gtgtatctcg atttggatta
     1141 gttgcttttg catcttcatt agaccaaatt ggtccattga ctcgaaatgt aaaagataat
     1201 gcaatcgtat tagaagctat ttctggtgca gatgttaatg actctacaag tgcaccagtt
     1261 gatgatgtag actttacatc tgaaattggt aaagatatta aaggattaaa agttgcatta
     1321 cctaaagaat acttaggtga aggtgtagct gatgacgtaa aagaagcagt tcaaaacgct
     1381 gtagaaactt taaaatcttt aggtgctgtc gttgaggaag tatcattgcc aaatactaaa
     1441 tttggtattc catcatatta cgtgattgca tcatcagaag cttcgtcaaa cctttctcgt
     1501 tttgacggaa ttcgttatgg ttatcattct aaagaagctc attcattaga agaattatat
     1561 aaaatgtcaa gatctgaagg tttcggtaaa gaagtaaaac gtcgtatttt cttaggtaca
     1621 tttgcattaa gttcaggtta ctatgatgct tactataaaa aatctcaaaa agttagaaca
     1681 ttgattaaaa atgactttga taaagtattc gaaaattatg atgtagtagt tggtccaaca
     1741 gcgcctacaa ctgcgtttaa tttaggtgaa gaaattgatg atccattaac aatgtatgcc
     1801 aatgatttat taacaacacc agtaaactta gctggattac ctggtatttc tgttccttgt
     1861 ggacaatcaa atggccgacc aatcggttta cagttcattg gtaaaccatt cgatgaaaaa
     1921 acgttatatc gtgtcgctta tcaatatgaa acacaataca atttacatga cgtttatgaa
     1981 aaattataag gagtggaaat catgcatttt gaaacagtta taggacttga agttcacgta
     2041 gagttaaaaa cggactcaaa aatgttttct ccatcaccag cgcattttgg agcagaacct
     2101 aactcaaata caaatgttat cgacttagca tatccaggtg tcttaccagt tgttaataag
     2161 cgtgcagtag actgggcaat gcgtgctgca atggcactaa atatggaaat cgcaacagaa
     2221 tctaagtttg accgtaagaa ctatttctat ccagataatc caaaagcata tcaaatttct
     2281 caatttgatc aaccaattgg tgaaaatgga tatatcgata tcgaagtcga cggtgaaaca
     2341 aaacgaatcg gtattactcg tcttcacatg gaagaagatg ctggtaagtc aacacataaa
     2401 ggtgagtatt cattagttga cttgaaccgt caaggtacac cgctaattga aatcgtatct
     2461 gaaccagata ttcgttcacc taaagaagca tatgcatatt tagaaaaatt gcgttcaatt
     2521 attcaataca ctggtgtatc agacgttaag atggaagagg gatctttacg ttgtgatgct
     2581 aacatctctt tacgtccata tggtcaagaa aaatttggta ctaaagccga attgaaaaac
     2641 ttaaactcat ttaactatgt acgtaaaggt ttagaatatg aagaaaaacg ccaagaagaa
     2701 gaattgttaa atggtggaga aatcggacaa gaaacacgtc gatttgatga atctacaggt
     2761 aaaacaattt taatgcgtgt taaagaaggt tctgatgatt accgttactt cccagagcct
     2821 gacattgtac ctttatatat tgatgatgct tggaaagagc gtgttcgtca gacaattcct
     2881 gaattaccag atgaacgtaa agctaagtat gtaaatgaat taggtttacc tgcatacgat
     2941 gcacacgtat taacattgac taaagaaatg tcagatttct ttgaatcaac aattgaacac
     3001 ggtgcagatg ttaaattaac atctaactgg ttaatgggtg gcgtaaacga atatttaaat
     3061 aaaaatcaag tagaattatt agatactaaa ttaacaccag aaaatttagc aggtatgatt
     3121 aaacttatcg aagacggaac aatgagcagt aaaattgcga agaaagtctt cccagagtta
     3181 gcagctaaag gtggtaatgc taaacagatt atggaagata atggcttagt tcaaatttct
     3241 gatgaagcaa cacttctaaa atttgtaaat gaagcattag acaataacga acaatcagtt
     3301 gaagattaca aaaatggtaa aggcaaagct atgggcttct tagttggtca aattatgaaa
     3361 gcgtctaaag gtcaagctaa tccacaatta gtaaatcaac tattaaaaca agaattagat
     3421 aaaagataat ttaaatcatc aaactatgaa gatttaaaaa ataaaccctt gattgctgac
     3481 ttagatgcaa tcgagggttt atttatatct atagaagtca aattactttt aactttattc
     3541 attgtacatg ttaatggtaa aaatattaat tttattaatg cgttagcttt aattatatta
     3601 aggcaaactg tataataaaa aggtataaaa catttttgta taaagacaac attatattta
     3661 caacatcatt ttaaaggtaa aatagcataa ctgacgaagt ctatataatg aagaacggca
     3721 aaaaatgctg aataaataac aagctttgta catattgaga tagtatttgt ttaagataca
     3781 agttggtctt taacgatatt aagaatgatg aaataagact gagcctgggt cataaattca
     3841 atgtcctagg cactacaatg ttatattggc tgtagttgac tgaaagaaaa tacgcttgta
     3901 acaagctttt ttcaattcta gtggggcccc aacatagaag ctgactttct gtcagcttac
     3961 aataatgtgc aagttggggt ggggccccaa catagagaat ttcgaaagga aattctacag
     4021 acaatgcaag ttggggtaga acgaaataaa ttttgttaaa tattatttct gtcccactcc
     4081 ctattagacg aaacaaagat gaagttaaaa tatatgaatt ttaagtagag ggataagata
     4141 tgaggaaacg tgctagaatc atttataacc cgacatcagg taaagagcta tttaaaagag
     4201 aattacctga tgccttaata aaattagaaa aagcgggata tgaaacgagt gcatatgcaa
     4261 ccgagaaaat aggtgatgcc acacttgaag cagaaagagc tatgcatgaa aattatgatg
     4321 tattaatcgc tgcaggtggt gatggaacat taaatgaagt agttaatggt atcgcagaaa
     4381 agcctaatcg tcctaagcta ggtgtcattc ctatgggtac tgttaatgac tttggacgtg
     4441 cattgcatat acctaatgac atcatggggg cacttgatgt catcattgaa ggtcattcta
     4501 ctaaagtaga tattggtaaa atgaataatc gatactttat taatttagct gcaggcggac
     4561 aattgacgca agtctcttat gaaacaccga gtaaattgaa atctattgtt ggtccatttg
     4621 cttattacat caaaggtttc gaaatgttac ctcaaatgaa agctgtagat ttaagaattg
     4681 aatatgatgg taatgttttc caaggagaag cattattatt ctttttaggt ttaacaaatt
     4741 caatggcagg atttgaaaaa ttagtgccgg acgctaagtt agatgacggc tattttacgt
     4801 taattatagt tgaaaaatca aaccttgcag aacttggtca tattatgact ttagcttcaa
     4861 gaggagaaca tactaagcat cctaaagtta tttatgaaaa agcaaaggca attaatattt
     4921 catcattcac agacttacaa ctaaatgtag atggagaata tggtggtaaa ttaccagcga
     4981 atttcttaaa tctagaacgt catatagatg tctttgcacc taatgatatt gtaaatgaag
     5041 agttaataaa taatgatcat gttgatgaca atttaatcga agaataaaca gaaatagtga
     5101 aatatataat ggcaggttag gaagcgcata attttgttcc taacctgttc attttggagg
     5161 taaaattttg caagcaatag ctaaaaatga cataaaaacg ggaacagttg ttgatttaac
     5221 gcatgaaggc catggtgtcg taaaaataga tcgctttcct attttcatac cacaagcttt
     5281 aattaatgaa caaattgaat ataaaattat caaagtaaaa aagaactttg cgataggtaa
     5341 gttgcttaat ataaatacga gaagtgagaa tcgtgttgca ccgccatgta tatattatga
     5401 acgttgtggt gggtgccaac ttcaacactt atcatacgaa gcacaacttg aaatgaaaaa
     5461 ggaacaagtg attaatttgt ttcagcgaaa agcgcatttt gataattcta agattaacga
     5521 tacagttggc atgacagatc cttggagata tcgaaacaag tcccagattc cagttggtaa
     5581 gaatgaacag aatgaagtta ttatgggctt ttatcgtcaa agaagccatg acattattga
     5641 catggaaagt tgtttaatac aagattcaca acatcaagaa gtgatgaatg aagttaaatc
     5701 tattttaaaa gatttaaatg taagtattta tcaagaacaa ttgaaaaaag gtttaatgag
     5761 acatttggtt gtaagaacag gatatcatac agacgaaatg atgattattt ttgtaactaa
     5821 tggtaaaaag tggccacaaa aaaatgctgt tgttgagaag atattagatg catttcctaa
     5881 tgtgacaagc attaaacaaa acattaatga tagccactct aatgttatta tgggacgtca
     5941 atctataacg ctatatggta aagatacaat tattgatcaa ttaacagata gtacatttaa
     6001 aataagtgat cagtcattct accaaattaa ttctgaacaa acagagaaat tatataataa
     6061 agcaattgag tatgcgcaat tgacaggtaa tgaagttgta ttagatacct attgtggtat
     6121 tggaacaata gggctatata tggcaccact tgcaaaacat gtatacggtg ttgaagtagt
     6181 accttctgca attgaagacg cacaacaaaa tgcgactata aaccaatgca ataatacaac
     6241 ttttgtctgt gggaaagctg aagaagttat tttacaatgg aaagcacaag ggattaaacc
     6301 agatgttgta atggttgatc cgccaagaaa gggctgtgac gaaacgttta ttcagacttt
     6361 gcttacatta gaacctaagc gtattgtcta tatttcatgt aatcccgcaa cacagcaacg
     6421 tgatgcatta ttattagctg aaaaatatca acttgaagag gtaacaccgg tagatatgtt
     6481 tccacaaaca acacatgttg agacagtggc attattcaat ttgaaatagc gcacatgatt
     6541 tgagataaaa tttcgcctta ataagtgaag aaagaagtcg tatattaaaa ttttattaca
     6601 gccaatttac tttcttgata tgaacttttt aaattaaatg tgacattgta tactatattt
     6661 aaagaagaat aagaatgtca tgatcgggag ggttggtaat gcataaaatt gatttaacga
     6721 caaataattt tcaaatgcga aggtttatta tcttgcaact tgttattgcg ttattcgtga
     6781 ttttatttac gtataagtgg gcgttaggtg tcacagcggt tgtcgatcaa aatatcatca
     6841 tcaatcttgt atatgggttt gctggattca ttattttatt gattttacat gaacttatcc
     6901 atagagcatt atttctttta tttaaaaaag atagcaaacc tatgtttaat ataaaaaaag
     6961 acagaatctt attccaaaca gcagatgctt gcttcaacaa atggcaattc tcaattatta
     7021 tgctgtcacc actaatccta ttaagtactg gcttgcttat tttaattaag gtatttggtt
     7081 attcatcact tatttttatg tttagtatgc ataccgctta ttgctttata gatattctgc
     7141 ttgttgcatt aacaatatct agtagtttta aatatgttca acaagatgaa gatagtattt
     7201 atttgtatca tcaaaagccg actcaataat aaattttaat atgatgatac caacaaaatg
     7261 aatttgcttg aattatttca gattacctat gttttcttac tcaatcgtct tcatcatcat
     7321 gcttaaatag caattgattt tataacaact tgtagttatc tcaatatagt tatcgctaag
     7381 tgcaagattt atgtaaaaaa atcgtacaca gcgtataatt atgttgagat atttttgtgc
     7441 gttataaaag taaaaattag tagggaggtg agcacttgac tgagagacga attattcata
     7501 tagatatgga ctattttttt gcacaagtcg aaatgagaga taaccccaaa ttgaaaggga
     7561 aaccagtcat tgttggtggt aaagcaagca gtaggggtgt tgtctctacg gcatcctatg
     7621 aagcacgaaa atacggcgtc cattcagcaa tgcctatgtc acaagcgcat aaattatgtc
     7681 caaatggata ctttgtcaca agtaattttg gtgcttaccg agaaacatct gcgcagatta
     7741 tgtctatttt ccgaagttat acagataagg tagaaccgat gtcattggat gaagcgtatt
     7801 tagatattac agaattagtg agaccagacc ttcctgcttc gaaaattgct cagtatatta
     7861 gaaaagatat tctagagcag acacatttaa cagcatctgc aggtgtttct tataacaaat
     7921 ttttagctaa attagcgagt ggtatgaata aacctgatgg tatgactgtg attgattatc
     7981 aaaatgtcca tgatattttg atgacattgg atattggaga ttttccaggc gtaggtaaag
     8041 cttccaaaaa agtaatgcat gataatggta tttttaacgg tagagattta tatgagaaaa
     8101 cggaatttga attaatacgt ttgtttggaa aaagaggtcg gggtttatat aacaaggcac
     8161 gcggtattga ccatagtgaa gtgaaatcat caagagtaag aaaatcagta gggactgaac
     8221 gcacatttgc aacagacgtg aatgatgatg aagagatttt aagaaaagta tgggaattgt
     8281 caggtaaaac agctgaacgt ctaaataaat tacagaagtc agctaaaact gtaacggtta
     8341 aaattaaaac ttatcaattt gaaacgctat ctaaacagat gagtttaaga gattcggtta
     8401 gttctgaaga agatatttat aatattgcat atttacttta taacgattta aaagaccctg
     8461 atgtaccaat tcgacttatt ggtgtcactg taggtaattt agaacaatca acttataaaa
     8521 atatgacgat atatgacttt atataaaata aagctccctg caaagtttac atttttacaa
     8581 tgcttacttt tgaagggagt attttattta gtcctagcct ttatctttta gatttttacc
     8641 ataaatatta gttacatatg ttaaattttc ataatttttc agtagcctaa aagtaatcat
     8701 tgcgcatgct ttggcatcat tcaatgcatc atgatgacca tgaaaatcta attgataaaa
     8761 ctccatcata tgttttaaac cgtatcgata cgaatcaacg gttcttttag caagttgata
     8821 actacaaaag taagttaaat ttggagttgg taaaccaata ttttgaatgc tttgatgtaa
     8881 gacgttcata tcaaatgccg cgttatgtgc gacaacaggt aaatctgcaa taaattgcat
     8941 catatatgga aatacgtagt caaacgtagg tgcattttca acatcttctg gttgtatgcc
     9001 atgaatttta atattttgtt gtgaaaaata gtcttgcgga ttcacaagag tatgaaatgt
     9061 ttctgttatt tgactatcaa tgactttaac cattccgaca gaacaaatac ttgtacgttt
     9121 accatttgct gtttcaaaat caagtgcaac aaacgcatct tgtatcatgt agtcaatcct
     9181 ttcaattaat taaatgtata tattaataga cttttagtca tttgcttaca aacgatacat
     9241 aatattatac gtgaaggtat aacgtgaaat ctatcatttg gatgtaactc tgattaaaaa
     9301 gtaaatgatg atatgcttta aaatcgtcaa attaattcaa catcaaattt agagttatta
     9361 acattagtag tttaaaaaat aaatgactaa aaatacaata attagccttg aaaatgaaaa
     9421 aacgcagatc aatgattcag aaaatgaatc tagtgatctc cgtaaagcaa aataatatag
     9481 acattgggtt attgtaagtg acccaaatgc ctatcatgtc gtatgatatt tatctgtcta
     9541 ttgtagtgat gtttaattat tcttcgtcga atgtacgagc gccaagttct ttttcgtaaa
     9601 gatataatgc attgctgtca tcgccgatac gagttaaata attgatgtga gtttcaaaca
     9661 ttgattcttc ttcgacttgt tcatctaaga accagtttaa gaatgagata gttgcataat
     9721 ctttatcttg acgagcgatt tcagataagt tatagaaacg tcttgttact tcttgttctt
     9781 gagataagct gtctttgaaa gtttctagta tgcttgaaaa gtcaattttt ggtgctgaaa
     9841 ctgctctgaa ttctgcatgt gcacctctgt cgttaatata gttataaatc ttttgtccat
     9901 ggaaacgttc ttctttagct tgttgaatga agaagtttgc aaatccttcg tacgattctt
     9961 tatcacagta tgctgccatt gccatatatg cgtgtgctgc aaagtactca tggttcattt
    10021 gatcatttaa tgcttctaat aaatttttac ttaacatttt gatacacctc ttatttgtaa
    10081 attaaaatta ctatgaatta aacttagtat aacaaactca ttctcaattg taaataataa
    10141 taattataat ttaatgcctt attttagcac tctaattgag aaaatgtatc atttagcgat
    10201 atttaattga gaataaatat catttttaat tatctaaatg agaatgatta taatttagaa
    10261 acactgtatc aatttgtgat tgagaaactt ttttgaagtt gccttgtcat gttataatga
    10321 tgcgagtgga aatttgagga ggaaacacat gagacagtgg acggcaatcc atctagcgaa
    10381 attggcgcgt aaagcaagta gagcagtagg taaaagagga acagatttac ctggacaaat
    10441 cgctagaaaa gtggatacag atatattaag aaaattagca gagcaagttg atgatattgt
    10501 atttatcagt ggaacaaatg gtaaaacaac gacttcaaac ttaattggac atactttaaa
    10561 agcaaataat attcaaatta tacacaataa tgaaggtgct aatatggctg caggtataac
    10621 ttctgcattc atcatgcaat caacacctaa gactaaaatt gcggtaatcg aaattgatga
    10681 aggttcgatt ccacgtgtgt taaaagaagt tacaccttca atgatggtat ttactaattt
    10741 ctttagagat caaatggatc gcttcggtga aattgatatt atggttaata acattgcaga
    10801 gacaattagt aataaaggca tcaaattatt gctaaatgct gatgatccat ttgtgagtcg
    10861 tttgaaaatc gcaagtgata cgattgtgta ctatggtatg aaagcacatg cccatgaatt
    10921 tgaacaaagt acgatgaatg aaagtagata ttgtccaaac tgtggtcgct tattgcaata
    10981 cgattatatt cattataatc aaattggtca ttatcactgt cagtgtggtt tcaaacgaga
    11041 gcaagcaaaa tatgaaatat caagttttga tgtggcaccg tttttatatt taaatatcaa
    11101 tgatgaaaaa tatgatatga aaattgcagg tgactttaac gcttataacg cgttagcagc
    11161 atatactgtt ttaagagagc tagggttaaa tgaacaaaca attaaaaatg gctttgaaac
    11221 gtatacatca gacaatggtc gtatgcagta ctttaaaaaa gaacgaaaag aagcgatgat
    11281 caatttagct aaaaatcctg caggaatgaa tgcaagttta tcagttggtg aacaattaga
    11341 aggcgaaaaa gtgtatgtta tttcgctaaa tgataacgct gcagatggtc gagatacttc
    11401 atggatttat gatgcagatt ttgaaaaatt atctaagcaa caaattgaag ctatcatcgt
    11461 gacaggtaca cgagcagaag aacttcaatt gcgattgaag ttagcagagg ttgaagtacc
    11521 aattatagtt gagcgtgata tttataaagc aacggcaaag actatggatt ataaaggttt
    11581 cacagttgca ataccaaact atacatcatt agcgcctatg cttgaacaat taaaccgttc
    11641 gtttgaagga ggtcaatcat aatatgcatg aattgactat ttatcatttt atgtcagata
    11701 aattgaattt atacagtgat ataggaaata ttattgcttt aagacaacgt gctaaaaaac
    11761 gaaatattaa agttaatgtc gtagaaatca atgaaacaga aggtattacc tttgatgaat
    11821 gtgatatttt ctttatcggt ggtggaagtg atagagaaca agcattagca acaaaagaat
    11881 taagtaaaat taagacacca cttaaagaag cgattgaaga tggtatgccg ggattaacga
    11941 tttgtggagg ctatcaattt ttagggaaaa aatatatcac gcctgatggt acagaattag
    12001 aagggttagg tattttagat ttttatactg aatcaaagac aaaccgatta acaggagata
    12061 ttgttatcga aagtgatact tttggaacta ttgtaggttt tgaaaatcac ggtggtagaa
    12121 catatcatga tttcggtaca cttggtcatg ttacttttgg ttatggtaat aatgatgaag
    12181 ataaaaaaga aggcattcat tataaaaatt tattaggtac ttatttacat ggaccaattt
    12241 tacctaaaaa ttacgaaatc actgattatc tgttagaaaa agcttgtgaa cgtaagggta
    12301 ttccgtttga gcctaaagaa atagataatg aagcggaaat acaagcgaaa caagtattaa
    12361 tagacagagc aaatagacag aagaaatctc gttaactctg aacatcgcat caatggattt
    12421 aatattgata aacgatgaag tttagtaatt aatcatatat gtataaacac acacattatt
    12481 ttggatggaa acaaccaaat tgatgtgtgt tttttgttct agtgaataat tattatacaa
    12541 tgagtatcta tcctagaatt atcaatagta atggtgatta tgcaacatga aaaaatgaat
    12601 gatgaaagga atttgacgat gaagcctact aaagtgatat taaaagatgc atcttattta
    12661 catagcaaaa catcgataac atttatttta aaagatgtag ttatcgaaga agataataaa
    12721 atttattatt tcgacactag tgcaactttc gaagatcaag aagttaaatt tgaatttgca
    12781 ctctttgata gtgacatgga caatttgaaa catatggatt gcgacaatcc tgttactgaa
    12841 atatatttta tagaacctga tttacatttt acaattattg attttaatca agaactgctt
    12901 tgtatttata ttgattttga ttctggttta aggcattcaa acatggcaac agaatctggt
    12961 atttcattaa ggataaatgt tgctaaatca gattttacta aatttattaa tgaattagcc
    13021 tctttacatt aatgatttaa atctgatatg taattacaat caaaaaagac agccacatcc
    13081 ctccgtagtt taggcgtgtg gctatatttg agtctgaata tttatgcttg taattttaaa
    13141 aagggacatg ctatatacga taaaaagagg cggggacata aatcaatgtt ctatgctcta
    13201 cgaagttata ttggcagtag ttgactgaac gaaaatgcgc ttgtaacaag cttttttcaa
    13261 ttctagtcag gggccccaac aaagagaaat tggattccca atttctacag acaatgcaag
    13321 ttggggtgtg ggccccaaca aagagaaatt ggattcccaa tttctacaga caatgcaagt
    13381 tggggtgtgg gccccaacac agagaatttc gaaaagaaat tctacaggca aagcgagttg
    13441 gggtgggacg acgaaataaa ttttatgaaa atatcatttc tgtcccactc ccatggtgcc
    13501 aattagcata aggtacttaa attaagcata tctgctgtct agcagtcgat aaatcattag
    13561 aacttcgtat agtatatgac ttttaatttg attttcacca ctaatttcaa gtgcttttat
    13621 agtcgaacgt aaagtttcta cagaatcatc ttctctctta aaagaaccat cataaaatat
    13681 atctttgatg ctactactaa tttttagcaa tgccattttt tcgtcacctg aaaagttaac
    13741 acgagtattt ttaggcaagt aaatgatatt tgataaatga gtgataaaca aacgattcgt
    13801 atatgcacgt ttagttaatt gattgagtaa tttccaatca cattcttttt tcttatgata
    13861 gcttaattca tcacgttgat aacttattaa cgtttcaact tgattattta aattgaaaat
    13921 atttttatat gctttttcgc ttttatcaga ttgcagtctt gataagataa gttcttggca
    13981 gcgattgtaa aataatttat acatcaaggc atctgtctta cttaattttt cttcgacctg
    14041 accataatac ttaggtggaa acaccatgaa gttaattaaa cctgatgtca cgagtccaat
    14101 aattgctgtc aatgttcgag acaaaaagtt gaatatgtag gcatcatgaa tacctggaat
    14161 catagctaat gatgttagta cagcgacatt cgtaccaact tgcaatttga gttttgtaca
    14221 gaataaaatc gtgaacgttg cactcaatgc atatgtaaaa ggtgattgat cgccgaataa
    14281 atatgtaaat aatactgcaa agcctgcacc aattaccgta gcaggtaatc tacgataacc
    14341 tttaataagt gatgccttgg cagttggttc aattgtgact acagctgtta aaatggcata
    14401 gatgggtgtt aaatctagtg ccatacaaaa gacagctgtt aaaaaaatgg caataccagt
    14461 tttaattgtt ctggcaccaa ttaaatgttt ataccattga tcgttcattt tttaacctct
    14521 aatcatcgta aaatcttagc gagcgcttta taataatagt atcgtacatt ggaaaagttc
    14581 atgtatgtaa aatatttgaa ataatcatac ataagcatta ctttgatttt catatacatt
    14641 aatcaaagtt ctgggctcca caatgttata ttgttagtag ttgactgaaa taaaatgcac
    14701 ttgtaacaag catttttcaa ttatagtccg gggccccaac atagagaatt tcaaaaaaga
    14761 aattctacag gcaatgcagg ttggcggggc cccaacacag aagctgacga aaagtcagct
    14821 tacgataatg tgcaggttgg cggggcccca acatagagaa attggatcta caatttctac
    14881 aggcaatgca agttggggta caacgataaa gaaatatttt ttctttatca cactatgtct
    14941 cactcacttt ccaaaatact aaagtaacat ctttagtata tcaaagaatt tttgctataa
    15001 taagttataa ttatataaaa aaggaacggg ataaaatgat tgtaaaaaca gaagaagaat
    15061 tacaagcgtt aaaagaaatt ggatacatat gcgctaaagt gcgcaataca atgcaagctg
    15121 caaccaaacc aggtatcact acgaaagagc ttgataatat tgcgaaagag ttatttgaag
    15181 aatacggtgc tatttctgcg ccaattcatg atgaaaattt tcctggtcaa acgtgtatta
    15241 gtgtcaatga agaggtggca catgggattc caagtaagcg tgtcattcgt gaaggagatt
    15301 tagtaaatat tgatgtatcg gctttgaaga atggctatta tgcagataca ggcatttcat
    15361 ttgtcgttgg agaatcagat gatccaatga aacaaaaagt atgtgacgta gcaacgatgg
    15421 catttgagaa tgcaattgca aaagtaaaac cgggtactaa gttaagtaac attggtaaag
    15481 cggtgcataa tacagctaga caaaatgatt tgaaagtcat taaaaactta acaggtcatg
    15541 gtgttggttt atcattacat gaagcaccag cacatgtact taattacttt gatccaaaag
    15601 acaaaacatt attaactgaa ggtatggtat tagctattga accgtttatc tcatcaaatg
    15661 catcatttgt tacagaaggt aaaaatgaat gggcttttga aacgagcgat aaaagttttg
    15721 ttgctcaaat tgagcatacg gttatcgtga ctaaggatgg tccgatttta acgacaaaga
    15781 ttgaagaaga atagttcaac atatactaag actaaagtat gaacatcatt tagttccgga
    15841 gcctattcat attggtttcg gaactgtttt ataataatta agaacacaat caatgcgtca
    15901 tttcaaaaat atgttgtaac aaagtagttt ttaagcaaac atatcatcga catcaacgaa
    15961 gatacatagc gcatttggta ttttaaaact tattataaaa ggtgatagtt atgaactatg
    16021 ttgaacgtta tattgaacag tttttgagag caacagtaag aaataatatc aagcactacc
    16081 ttttaatgct agatgaaaaa atgaaaaatt tagatgatta tatgcgttat ttaattacta
    16141 aaaaagaaca acttagcaag ttaattgaca gtctaatgct aacattagaa aataaatata
    16201 ttgatattgc tgaagcattt caaattcaat gtgcaagaga aatcaataat caagaaattg
    16261 aaaatattaa atcagagttg aataaagttg aagcatatta tgcacaaatt gaaactcaaa
    16321 ttcaacaaac ttcaactgaa aaaatagcaa cagaaaaaac atcgtatcta ataaattata
    16381 tgaacgctgt ggcatagaaa ggcggcgaaa catgacacac aaatatatat caacgcaaat
    16441 gttgatcatt tttactgcat taatgattat tgccaatttt tactacatat tttttgaaaa
    16501 aattggcttt ttactcgttc tattattggg atgtgtatta gtttatgtag gatatcttta
    16561 ttttcataaa atacgtggcc ttttggcgtt ttggataggc gcgctattaa ttgcattcac
    16621 attattgtct aataagtata caatcatcat cttgttcgtc tttttattat tacttattgt
    16681 gcgttattta atacacaagt ttaaaccaaa aaaagtagtt gcgacggatg aggttatgac
    16741 ttcaccatct tttattaaac aaaagtggtt tggtgagcaa cgtacaccag tttatgtata
    16801 taagtgggaa gatgtacaaa ttcaacatgg aattggcgac ctacatattg acttaacaaa
    16861 agctgcaaat attaaggaaa ataataccat tgttgttaga cacattttag gtaaagtgca
    16921 ggttatattg ccggttaatt acaatattaa tttacatgta gctgcttttt atggaagtac
    16981 ttacgtgaat gaaaaatcat ataaagttga aaataacaat attcatattg aagaaatgat
    17041 gaaaccggat aactatacag ttaatatcta cgtatcaacg tttatcggag acgtagaggt
    17101 gatttatcga tgaaccacta cattagaaca attggttcaa tgctcatctt agtatatagc
    17161 atgctagctg catttctgtt catcgataaa gtttttgtaa atatcatcta ttttcaaggt
    17221 atgttttata cacaaatatt cggaatacca gtttttttat ttttaaatct catcatcata
    17281 ttattgtgta ttattgttgg ttcggtactc gcttacaaaa tcaatcagca aaatgattgg
    17341 attaagacgc aaattgagcg ttcaatggaa ggcgaaacag ttggcattaa tgatcaaaat
    17401 atagaaatat atagtgaaac gttagattta taccatacac tcgtaccttt aaatcaagaa
    17461 ttgcataagt tgcgacttaa aactcaaaac ttaaccaatg aaaattataa tattaatgat
    17521 gtgaaagtta aaaagattat tgaagatgaa cgtcaaagac tagcacgaga acttcacgat
    17581 tctgttagtc agcaactttt tgcggcaagt atgatgctat ctgctatcaa agaaacgaag
    17641 ttagaaccac cattagacca acaaattcct attttagaga aaatggttca agattcgcag
    17701 ttagaaatgc gtgctttgct gttacattta agaccgcttg gtttaaaaga caaatcttta
    17761 ggtgagggta ttaaagattt agttattgat ttacaaaaaa aagtgccaat gaaagttgtg
    17821 catgaaatac aagattttaa agtgcctaaa ggtattgaag atcatttgtt cagaattaca
    17881 caggaagcaa tttcgaatac attgcgtcat tcaaacggta caaaagtgac agtagaattg
    17941 tttaataaag acgattattt attgttgaga attcaagata atggtaaagg ttttaatgtt
    18001 gatgaaaaat tagaacaaag ttatggactt aaaaatatgc gtgaaagagc tttggaaatt
    18061 ggtgcaacgt tccatattgt atcattgcca gattcaggta cacgtatcga ggtgaaagca
    18121 cctttaaata aggaggattc gtatgacgat taaagtattg tttgtggatg atcatgaaat
    18181 ggtacgtata ggaatttcaa gttatctatc aacgcaaagt gatattgaag tagttggtga
    18241 aggcgcttct ggtaaagaag caattgccaa agcccatgag ttgaagccag atttaatttt
    18301 aatggattta cttatggatg acatggatgg tgtagaagcg acgactcaga ttaaaaaaga
    18361 tttaccgcaa attaaagtat taatgttaac tagttttatt gaagataaag aggtatatcg
    18421 tgcattagat gcaggtgtcg atagttacat tttaaaaaca acaagtgcaa aagatatcgc
    18481 cgatgcagtt cgtaaaactt ctagaggaga atctgttttt gaaccggaag ttttagtgaa
    18541 aatgcgtaac cgtatgaaaa agcgcgcaga gttatatgaa atgcttacag aacgagaaat
    18601 ggaaatatta ttattgattg cgaaaggtta ctcaaatcaa gaaattgcta gtgcatcgca
    18661 tattactatt aaaacggtta agacacatgt gagtaacatt ttaagtaagt tagaagtgca
    18721 agatagaaca caagctgtta tctatgcatt ccaacataat ttaattcaat agttcatatc
    18781 gaattaagaa aagttactta cgccaatcac aatataacat caaatagaca ccaaaaaaca
    18841 gaggttttcg caataatcat tagcgaaaac ctctgttttg ttggtgttta gtctttttta
    18901 agcttatcaa cgattttgtc ttttatattt ttatcatctt ctggataggt atcactagga
    18961 tgttcatcaa catgatacgt acgttgatgt gttgcattac tatcttcatc atattcgtat
    19021 ctagtgttct cattataatg atttttatta ttgtcatcat gttctaatgc tgcttcttca
    19081 ggtgttttac ctttaattac actacgctga tgaatgattg cattgatttc agcaccgaca
    19141 attataataa aacttgtgat atataaccat agtaacaaaa tgatgatacc cgcgatactg
    19201 ccatatgttt tagaatagtt accaaaattt gaaatatacc aaccaaaacc aaatgaacca
    19261 gctaaccaaa taattgaagt aaatactgca cctggtaata ctgacttaag cttcgtttta
    19321 acgttaggtg caaccgaata taacacgata aatatgataa atataataat gattggtaac
    19381 acaattctaa taaggttaaa aatccatttc acttgttcgt caaatccaag tggaccgaat
    19441 aggaaatgac taattacaga accaagcgtt ggtaatgcta gagcaactac aaacacaacg
    19501 cccataacta cagtgaagac aacacttagt agttttaata cgattccatt acggctatct
    19561 tctacatcgt aagcaacatt gaaagaattc ataattgcag tcattccatt tgaagctgac
    19621 caaattgcta aaatcaaacc gatagataat aagccaccac tggagttttg agttatatca
    19681 ccaattacac tcttaattag agtagatgtt tcagcgggtg cattgcttaa catattagta
    19741 atttgactct gcttaatatt gaaaaatggt aataatgtta ataggaaaag caacataggg
    19801 aacatagcaa gtacgaaatg gtaagtcatt tgtgctgcta gtcctgaggc atcatctttt
    19861 cctatgcgat agatgagata tgataagaaa ttagagtctt tcttatattt tgcaggttta
    19921 tttaatcttg ataagaagaa aacctgatca tcttttttag gtttcttaga ttggaattgt
    19981 tgaggttcaa tatatgtacg atcaacatca attttgggat tacttttatt tttctttttt
    20041 gactcctctt gcgcttcctt aacagaatta aggtattttg aagaagagtg atcctttttc
    20101 gacatgtgca ctccttaacc tttctctatg taatgtttta taaagtaact attagtattt
    20161 atccaattat aaaaaaacta aacaatgata tgctttaaaa atgataatat tatagaaatt
    20221 aattgattta aatctttcat aggctatttt gatttaaaat taaaacaaag gcaatactga
    20281 aacaatgcag tttggccaat cattgtatta tcatttaata atgataacta tgaaaggcgt
    20341 acaccaaatt gttaagtatt gccttataaa aacaaaatca tcttagcgtt gattttttct
    20401 attaacaaat gtttctttcg catcctttaa tgaacgttct aattcaggat tattacgacg
    20461 aatttcttca acaacatctt tccagtataa aacttcgtct ttaatcttgc tgactttaga
    20521 aggcttacga gtgcggttac cattttttgc atctttaact gattgaacta aagcttgacg
    20581 tgtagattta tcagctaaac taattgcacc accaattacg gcaccaatta aaataccagg
    20641 aacaaattta ttttccataa aaaactaccc ctctttcaaa tttgcatctt ttacgatgta
    20701 gtctattaaa ttatcacaag atgataatac catgtcgtat acaccttcaa aattattcgt
    20761 gtagtatgga tctggtacat cactctcttc catattacta aattctaaca gtttgaacaa
    20821 ttgtccctta agattaggat tgatagattt aatattatca acgttacttt gatccatagc
    20881 cacaatgtaa tcaaaatcat ctgtcgcttc gaataattca ctaatcatgc catcaaatgg
    20941 aatattgtgt ttgttgagaa ttttttgtgt accttcatga ggtggctctc ctaaattcca
    21001 gctaccagta cctcttgaat gtactttaat atcatgaata tttctgtctt taagtctttg
    21061 tcgcatgatt gcttctgcca ttggagaacg acatatattg ccaagacaga caaatgctac
    21121 atctaccatt ttgattcctc caaactatgt agttatatcc ccattttata gcgactttaa
    21181 acaataagaa agcagattat ataaaattct attaaagttt attaaattgt gatactttga
    21241 taacataact attattagag gtgaacattg tggctatgac aaatgaagag aaagtattag
    21301 ctattagaga gaagttaaat attgttaatc aaggattatt agatcctgaa aaatataaaa
    21361 atgcaaatga agaagaatta acagatatat atgattttgt tcaatcaaga gaaagattgt
    21421 cgccaagtga agtgacagct attgctgacg ctttaggaca attgcgacac gaataggagt
    21481 gggaattttg actaattaca aagaaaagtt acaacaatac gctgaactat tagttaaggt
    21541 tggtatgaat gttcaaccaa aacagccagt atttattaga tcatcagtag aaacattaga
    21601 attaacgcat ttaattgtag aagaagcata tcattgtggc gcttcagatg tacgtgttgt
    21661 atatagcgat ccaaccttaa aacgcttaaa atttgaaaat gaatctgtgg aacattttgc
    21721 taatcatgag ataaaatcat atgatgttga agcgagaatg gattacgtta aacgtggcgc
    21781 tgctaattta gcacttatta gtgaagatcc tgatttaatg gatggtatcg atagccaaaa
    21841 attacaagca tttcaacaac aaaacgcacg cgcatttaaa ggatatatgg aaagtgtaca
    21901 aaaaaatcaa tttccatggg tcgtagctgc ttttccatct aaagcttggg cgaagagagt
    21961 gtatcctgaa ctatctgtag aagaagcata tattaagttt attgacgagg tattcgatat
    22021 tgtgcgaatt gatggtaacg atccagttga aaattggcgt caacacatcg ctaatttaag
    22081 tgtttacgct caaaagttac aacaaaaaaa ttaccatgcg ttgcattatg tatctgaagg
    22141 tactgattta acagtaggtt tagcgaaaaa tcatatctgg gaagatgcta caagttatgt
    22201 taacggcaag gaacaagctt ttattgctaa tataccaact gaagaagtat ttacagcacc
    22261 agatcgaaat agagtagatg gatatgttac taataaattg cctttaagtt ataatggcac
    22321 aattatcgat caatttaaat tgatgtttaa agatggagag attattgatt tttcagctgg
    22381 aaaaggtgaa gcggtattga aagatttaat caatactgat gaaggttcaa gaagattagg
    22441 tgaagtagca ttagtacctg atgattcacc gatatcaaat cgtaatacca ttttttacaa
    22501 tactttattt gatgaaaatg cagcatgtca tttagcgatt ggatctgctt acgcctttaa
    22561 tattcaaggt ggaacggaaa tgactgttga ggaaaagatt gcaagcggat taaatgattc
    22621 aaatgtacat gtcgatttca tgattggtag tagtgatttg actatttatg gcatattcga
    22681 agatggttca aaagaactag tatttgaaaa tggaaattgg gcatcaacat tttaataaat
    22741 gttattttga ggtgctgagt aggaaatgaa acatgtattt aaaggtcaaa tacgtgatta
    22801 aagtatagat tgggagataa aataatgaca aatcaggaca gaccaatgaa atctatgtca
    22861 gaatcaaaat gttataaaaa tagacaagtt ttccctcaag atacgaatca ccatcataca
    22921 atgtttggtg gtacattgat ggctaatatt gatgaaattg cagcaatcac agctatgaaa
    22981 catgctggtg cgcaagtagt taccgcatct acagactcag tagatttctt aaagccgatt
    23041 aaaacagggg acatattaca atacgtagcg atggtttcat acgctgggac tagttcaatg
    23101 gaagtggtcg ttcaaattag aattgacgat gtatttaata acaagcatga tttagctgca
    23161 ttaagttact taacatttgt tgcgttagat gatgaaggca aacctaaaca tgtacctggt
    23221 gtatatccag aagatgatgt tgaaaaatgg ttttatgata cagcaccaca acgtgttgaa
    23281 cgaagaaaag cacgtcgcat tgaaagtaaa caaacaattg aatatttagc tcaagtgcaa
    23341 catattagag actaaaaata taaagacaga ttgttaagga tgaattgaac tgttatgttc
    23401 gaatattcca cctacaatct gtctttttga ttatgcacgt ttatgctttt agttaaaatt
    23461 tacccctttt attatagact attagtccta ttaaatgccc tttattattt aagtagagta
    23521 ctgccagcaa ttaattgttt tttattttat aatagcatta aaatatgcgg agactactta
    23581 tcgataaagt gcttctccgc ataattaaga gtttttaaaa ttgtataatt tgtggttgtt
    23641 tgtgcatatg acgctcatta tctttaaaat ccatattcgg atagtacata tttttaacaa
    23701 gaacatttgg tcctaaacaa ctaaactcgg aacaatgaca atttaatgat ttagcaagat
    23761 cagaggataa ccatttatca aatacatctg ttaatttatc tttttgtata ttcgaaattg
    23821 tacctgtttc atctccgaaa tcagttacga ttacattacc tgtaaataca ttgacattta
    23881 aacgactacg gccatccggg tcatttctag tcgttacatt tttagcattt cttaaacgtg
    23941 atagtaactt ttgatcttca tcatccttta agcatggaaa cacaggcaaa gtaccaaata
    24001 acatccaaat atcttcatct ctgaaatcca atatatcatg aattgtcttt ttcatttccg
    24061 ctagagttaa cacatttaat tgacttgcaa agtcagctgg atacataggg tgaatctcgt
    24121 gtctgctaca tttcatatca tgaacgactt cttgatgtat ttttcgtaaa tgtggtagcg
    24181 tactttgatt gagcattgtt tccgcagata caaacattcc ttgttctgat aatgtacgtg
    24241 cattcgaaat catttgttca tataatttta acttagcttt taacggtggt tgcttcttca
    24301 ttgcgccaaa gccaacattt gcgaattcat cagttgttcc ccagttgtgt gagatatgca
    24361 taacatcgat atattcagca atatctaaat aacgatcttg aggcaatgtt aggtttgaat
    24421 tcatttgtgt atatatacct cgatgatgtg catactttaa tagaggttta acaacatttc
    24481 taatagactt tttagaaaac attggttcgc cacctgtaat agacatcgtt cgcagatttg
    24541 gaatttcatc aagtctacga taaattaagt ccatatctaa aggctcgggg tcgacagttt
    24601 gtaaagtata accaactgca cagtggctac aacgcatatt acaaagattt gtagttgtaa
    24661 attcgatgtt acttaaagtt aattggccat gttctttaac atcgttatat gcttcccatg
    24721 gatcattgta aatgctaaca ggttgcttca atttgacgct attcatcgtc ttaaacttcc
    24781 ttctttctat ttggttcata tcggtatgat accacttcgt attatcactc gtaacaaatt
    24841 atctcttaat tttagttaat tgaaaagggg atttttaaaa gtcaaaataa tttgaatgtc
    24901 ataaatatag ctaagattat atcgctttaa cgcaatttct gttaggataa taatgaaaaa
    24961 gaaaacaaaa taaaaggagt taacttttta tgaatgaaca acaaacaatc gaacagataa
    25021 aagcgcgttt aaataagttt attgaggata tcgatcatgt aaatcctgat gaagtacgtg
    25081 ttgaagatat agatgaatgg attggattgt tagatcagct tgaagaaaag gttaaattag
    25141 tatctaagta aatgccacat aagatgtgtt agggttagat atcgcgacag tcgcttaata
    25201 ttttttatat cttgtatgtt taatcactga tttacttgtc tatagtaatt atatcaatga
    25261 tatatagaaa ggtgatttgc aatgactaaa aaagtagcaa ttattctagc aaacgaattt
    25321 gaagatatag aatattcaag ccctaaagag gcattagaga atgcaggctt taatactgta
    25381 gtgattggag atactgcaaa tagtgaagtt gttggtaaac acggtgaaaa agttactgtc
    25441 gatgtaggca ttgcagaagc taaaccagaa gattatgatg cattattaat tcctggagga
    25501 ttttcaccag atcatttacg tggagataca gaaggtcgat atggcacatt tgctaaatac
    25561 tttactaaaa atgatgtacc aacatttgcc atttgtcatg ggccacaaat actaatagat
    25621 acagacgatt taaaaggtcg tacgttaaca gcagtattaa atgtacgcaa agatttatca
    25681 aatgcaggcg cacatgtagt tgatgagtca gtagttgtag acaacaatat tgtaacaagt
    25741 cgagtaccag acgatttaga tgattttaat cgagaaatcg ttaaacaatt acaatagaag
    25801 tattgtggtt atcgattgtt aaaatttata tattttcgtt tgaaatgaaa tatggatttt
    25861 agttttaata aatgaataac aaataaaagg tcgtctctat tggcatttaa tagggatggt
    25921 cttttatttt tataaaatat aacaatattt gacatgatta atatttaaaa ttgcaatgat
    25981 ttatctataa ttttttcaaa aaacaaaaaa gtaagtatga tggcgtatac gattgtaagc
    26041 ccttggactg attttcccaa aaagtgtagc ctatttgtga tagtactggt aaactcaagg
    26101 tatatactaa gtgagtttta aaagaaggag caaacgcatg aaaagaagcg ataggtactc
    26161 aaactcaaat gaacattttg agcatatgaa acacgaacct cactataata cgtattatca
    26221 accagttggc aaaccgccga aaaagaaaaa aagtaaacga atactattaa aaatattatt
    26281 aaccattcta atcattatcg cattgtttat tggtatcatg tattttttat ctacacgcga
    26341 taatgtggat gaactaagaa aaattgaaaa taaaagtagt tttgtgtcag ctgataacat
    26401 gccagagtat gttaaaggtg cctttatttc aatggaagat gaacgattct acaatcatca
    26461 tggattcgat ttgaaaggta caactagagc tttattttca acgattagcg acagagatgt
    26521 gcaaggtggt agtaccatta cacaacaagt tgtcaaaaat tatttttatg ataatgatcg
    26581 ttcatttact agaaaagtaa aagaattatt tgtagctcat cgagttgaaa aacaatataa
    26641 taagaacgaa attttaagct tttatttaaa taatatttac tttggggata atcaatatac
    26701 gcttgagggc gcagcaaacc attactttgg aacaaccgtg aataaaaata gtacaacaat
    26761 gtctcacata acagttttac aaagcgctat tttagctagt aaagtcaatg cacctagcgt
    26821 atataatatc aataatatgt cagagaattt tacgcaacgt gtaagcacga acttagaaaa
    26881 aatgaagcaa caaaattata tcaatgaaac acaatatcaa caggctatgt cacaattaaa
    26941 tcgttaaata cttgcattca tgtgtaagat gcttggaact ataatgtaga aggtttaatt
    27001 gaaatgtcat aatgataaag ttgaattgtt tctgctttat aaattttaca gatttgcttg
    27061 taatcacact ttcattttaa aagatgatag tgtgattttt tgttagcatc gaaaaatatt
    27121 taaagttaaa catttgctat aggaagtggc ttattgaata ataaaagagt attgtattaa
    27181 aacttaagaa agtaacgggg tgatccagtg ccgaagatta ctaaaataga agttcaaaag
    27241 aaaaacaaag aacggtttaa tcttttttta gacgaacaat ttgaaatggg tatagatatt
    27301 gatacattag tcaaatttaa tttaaaaaaa gggcaacaac ttgaagctgc tgacatggca
    27361 gagattcaaa agtatgatca ttatcgcata ggtttaaata aagcaatcca atatttatca
    27421 tataaaaaga gaactgaaaa agaagttata caatatttac aaaaagaaga gatatcagag
    27481 caagcgattt ctgaagtgat tgaatattgt tatcgcgaaa agttaatcga ccatcaagat
    27541 tatgcggaaa gtttaaaaaa tacaatgatt cgcacgacag ataaaggacc taaaatttat
    27601 caacaaaaac tatatcaact tggtatagaa ccaaatatca ttgaaatatt tacagaactt
    27661 tatagagaac aacaggaact tgatgatatt atccaaattg ctgaaaaaat atctaaaaca
    27721 aaaaaagggc cacaaaataa agttaaagaa aaagtaatgc aatctttaat tcaaaaaggg
    27781 tttgaaatgg aaaccattca tgctgtattg aatgaaatgg attttacaca agatgaagcg
    27841 gttttagacg atttattaca acgagattta gaaaaaattt ataataaaaa tcgaaagaag
    27901 tacacgcaac agaaattaat ttcaaaaact atcgaaggcc ttatgagaaa aggatataaa
    27961 tatgataaaa ttaaagctaa attagaagaa agtggtattg ccgatggaac agaagaaatt
    28021 gagtgagatg tctgagccag aattaagaca cgaaatacaa ctttataaag agaaaatgag
    28081 aaaagctgag atgaatggta ttttaaatga atatgacgtt tatcaaagta aagtaattgt
    28141 tgccgaaagt tacttagtag acagaaaaaa gatagaaatt ggtaaaattt ataaactgac
    28201 tgatggcagt aatcaatatt ttaaagtaga aagattaaaa ggtatttttg cgtgggggtt
    28261 tagatttaat agcgatgaac ccgaagaagg attacctata gcgttattac aattatagaa
    28321 ggatgaaaat agatgggaag ttcaattgtt ttaaagttac taaaagtaac acactactat
    28381 aggaataaac agaataagaa atggtattta ccttttggat atgatgctga agatatcgat
    28441 ttaaacaata ttagtttaca tatttatcaa ggagaagcat taggtataat tggtgaacct
    28501 gaatcttcca aagcgttggt aggtcaattg ttggcaggtg caattaaacc tgataaaggt
    28561 aaagtagttt gtactgaaga tttgttctac ggatatattg aagatcaatc gttaattcat
    28621 caaactgttg aagcttatac agcgcagtta gttcaactat ttccatatga aattaatgat
    28681 cataaagctg aacagattat tcaatatgca catttaggtg attataaaac gaagccggtt
    28741 aaccatattt cgaaagcggc atacgctcaa ttactattaa gtattgcacg ctcatcaaaa
    28801 tcaaatatta ttattttaaa tcatgttatt gactatttaa caccacaatt tatggaacgt
    28861 gcgattgaat taacaaatga ttatattgaa aataatttaa cgattgtgtc aattggtgat
    28921 gatattgata aaatttcaca agtgagtaac tacatagctt ggttttcaca tgatcaatta
    28981 agaatggaag ggtcacttaa acaagttatt ccatctttta aagaacatga acgtgatcga
    29041 ttaagtctaa actcaaaaga agaaattgaa aactttgatt tagattggaa aaagaatcgt
    29101 acaagaatac cagagatgac ctataatttc aaacgtgttg agcgctataa tcatgcaaaa
    29161 ccgcctaagt ttttagtgcg tttttggact ttggcctcag gtactatttt aggcttagca
    29221 ttgatgatgt tgctcatttt caataatata ggaattattt cgataacaga ttttacgaat
    29281 cgtgctacga tgcaaaatga aaataaagat ccatatggcg aaaagttagc ttatggaatt
    29341 gcttttaatg gcagtgtgga tatgcaaggg gataaacaag tcacaattcc aaaatatagt
    29401 gtagttacaa ttactggcga aaatagtaaa aattatcgtg ttaccgccga taataagact
    29461 tactatgtta gtaaagataa attagaatat tttaacccgg caggtttata tcaaacgcat
    29521 agttttaaaa aattagcacc atatatgaaa tcaaattata gtaattacta tgcatacttt
    29581 aatagtcaat tacataaaaa gcatagttca gttataaaaa ctttagttcc tgatgatgat
    29641 aaccgtttcg ttgcatccgt tacacaacaa ccgatacaat tacttttcaa tgataataat
    29701 cagttatacg gttttgttta tccaattgta gataaaaaag aattaaaaga taagtttaat
    29761 attaacaata acatttggat tactaaagtt gggaatggat attgtattgc caatttgaaa
    29821 gaagacaaat ggatttatat tgaattgtag gtgtaaagat gctagataat attattttat
    29881 attttaaaaa cttaccgcat actaaaaggt atgtaacaga acgtttaaaa caaagttgga
    29941 aaagttttct aatcgtatta gctgcatgtt taatacttat tattgcaagt gaaacgctat
    30001 tttcctttag tcatttgacc gatgttaaag aggtgagatg gttatttaga atcattgtat
    30061 tgattgtttt tgcggtagtg atgttcacaa tttatatctc ttatcatcat tatatgaatg
    30121 actttttagt tactaagtta tttaatattt ccgcagcgac gccagtagtt attatgtcta
    30181 ttttaagttt tattatgcta gtcattttga cgatgattag tgcattggtt aaaccagtaa
    30241 acttcgaaac atcatatata gcgctattct attttattgt gatggcaact atttttgtag
    30301 gattaatatc cgttactttt ggtcttattc gactgttaac tgaaaaaata aatatcatat
    30361 tttatggggt ttgtgtattg tgtttcttat tgttaccaat tattttcata ccaaatccaa
    30421 atcatgtatt tatcaatcac atcctaatgc taaatccaat gtattacatt gttaatggta
    30481 tagcacaatc tatcatattt ggtatatcaa gtatggaaaa cattccatat catttttact
    30541 ttattttatt cttatgttta atagctgcag taaatttcgt attagcaagg tatacgacac
    30601 acgcaattta taataaaaca tctaaagtga cacaaactga taatcaacag gatgtttcaa
    30661 atgatagtac agacgaggcg gatacttctt catgatgttt agctgacctg ttgtgtaaat
    30721 taatctgtga gaatcagaaa caattagaat atcatctaga tattgttttc ctatactgat
    30781 tttgatattt cgagtcatta aaggtagttt gttagcaaca atataaattt tagattttgt
    30841 aaaagcattc atagttttac attaaaaaac cggacagagt aaaaatcgtt ttgattttca
    30901 ttctgcccgg ttgttcattt tttatagatt aactttagtg aataataact tgaaattatt
    30961 tagcatacag tttacgttgt aacttatctt ctgtaaatac ccagccaata taagagtggt
    31021 ctatgacatt atcgtttgtt acatgtgcaa ttgccacaaa tgaataatga tcatttttta
    31081 aataacgcaa atcaatcaaa cgaatttcag tagaaccatc tgctaacgtt gttgtttgcc
    31141 aacgatagat tgatgaaaag tttaaaaaag tacgtatatc tttattacct ttgactttcc
    31201 ataaaatgga gtctgttgat aatgtttgac gttccacttt atcactaaac accacattcc
    31261 taccatatgc ttttccaaca tagtcatgtg catcagtttg tatcgcgaca cgccattcca
    31321 taaattttat tgttggcgca acaaatactt taacagggtg atgctcttgt tgaattgctt
    31381 ttaaagcttg ttgcttaatt acggctctca ttttaaaacg aatcatgtaa taaatgatta
    31441 gtaaagcgat tataggaaag aagactgcaa atggatgcaa gcctataacc cataatacaa
    31501 taccaataca aagaactgtg aaaataatag ggtcaaatgt gttaatcaca cttaattgaa
    31561 tccatttgtt tgtgataggt ctaagcgctt gtgtaccata agaattgaat atatctacaa
    31621 agacatgtaa aaatactgcg agctgagccc acatccatac atgaaatggg ttggttccac
    31681 taaagaacgt gaatattaaa aatgtaatta aaattggcca tagtattgtg aaagggatgg
    31741 aatgcgtgat acctctatga tgcgaaatat atgttgcatt gtcctttaat ttaagaacag
    31801 tatccccatc aggaattaat gaaccaacga taagggttgt agctgttgca ccaaacgtag
    31861 aagccattgc tggatcttga gttgcaagtg ctgtaaggcc caccccaatt gcgatatgtg
    31921 ttgcagtgtc catatcagtt cactcatttc ataatattat atatactatt ataatccaat
    31981 ttcgcaaata actaaattaa cttacatact aaccatgatt ttaatgtaca ataaaaacat
    32041 tatgagtaaa gacaattaat tttggtgagg aaagggtttt ataaatgtat cagcagtcta
    32101 gttttaaaga aaatttgata cactggtttg atgaaaatca aagagaaatg ccatggcgac
    32161 aaacgaccaa tccgtactat atttggttaa gtgaggtcat gttacaacag acacaagtta
    32221 aaacagttat tgactattat catcgttttg ttgaacgatt tccaactgtg gaagttttga
    32281 gtcaagcttc ggaagatgaa gtgttaaagt attgggaagg cttaggatat tatagtagag
    32341 ctcgaaattt tcatacggcg attaaagaag ttcatgataa atatgaaggc ttagttccca
    32401 aagatcctga tcaatttaaa gcgttaaaag gtgttggccc atacacacaa gctgcagtaa
    32461 tgagcattgc atataatgta ccactagcaa ctgtagacgg aaatgttttt cgagtatggt
    32521 ctagattgaa tgatgactat cgagatatta aattacaatc gacgaggaaa tcgtacgaac
    32581 aagaattatt accgtatgtt actacagaag ctggaacgtt taatcaagct atgatggaat
    32641 taggggcgtt aatttgtaca ccaaaaaatc cattatgctt attttgtcca gttcaagaaa
    32701 actgtgaggc atttgataaa ggtacatttg aaaagttacc tgttaaatct aaaaatgtaa
    32761 gtaaaaaagt aattgagcaa tccgtctttt taattagaaa taatcaaggt caatatctgt
    32821 tgcaaaagcg tagcgaaaaa ttattgcatg gtatgtggca atttcctatg tttgaaagtg
    32881 agcatgctag acgtaaaatg actgaaaaaa tcggtcatga cattcaacca gtagaaacac
    32941 ctatttttga gttgaagcat caatttactc atttaacatg gaagattaaa gtatacgccg
    33001 tttcaggagc aattaacatt gaaacattac cagatgacat gatttggttt gatttaagtg
    33061 atcgagatca atatacattc cctgtaccta tgtcaaagat ttatcaattt ataaatgggt
    33121 aacattcgat gattggtaat attatcggaa aacatagtga cattaatttt aaaaagtata
    33181 aatgatatct ttaaatctca tagaataaca atgtcaaaaa gt
//
LOCUS       DAKTLH010000015        30902 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.015, whole genome shotgun sequence.
ACCESSION   DAKTLH010000015 DAKTLH010000000
VERSION     DAKTLH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 30902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30902
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.015"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            52..546
                     /locus_tag="O0394_000641"
     CDS             52..546
                     /locus_tag="O0394_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HCY0498741.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            complement(907..1875)
                     /locus_tag="O0394_000642"
     CDS             complement(907..1875)
                     /locus_tag="O0394_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCY0498742.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVSESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(1930..2970)
                     /locus_tag="O0394_000643"
     CDS             complement(1930..2970)
                     /locus_tag="O0394_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCY0498743.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            complement(2995..4071)
                     /locus_tag="O0394_000644"
     CDS             complement(2995..4071)
                     /locus_tag="O0394_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCY0498744.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRADSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHHAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            complement(4145..4984)
                     /locus_tag="O0394_000645"
     CDS             complement(4145..4984)
                     /locus_tag="O0394_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCY0498745.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            complement(4986..6254)
                     /locus_tag="O0394_000646"
     CDS             complement(4986..6254)
                     /locus_tag="O0394_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCY0498746.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYIINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            complement(6257..7528)
                     /locus_tag="O0394_000647"
     CDS             complement(6257..7528)
                     /locus_tag="O0394_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCY0498747.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            complement(7541..8638)
                     /gene="ugpC"
                     /locus_tag="O0394_000648"
     CDS             complement(7541..8638)
                     /gene="ugpC"
                     /locus_tag="O0394_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCY0498748.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            complement(9021..9599)
                     /locus_tag="O0394_000649"
     CDS             complement(9021..9599)
                     /locus_tag="O0394_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCY0498749.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGIQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            9808..10434
                     /locus_tag="O0394_000650"
     CDS             9808..10434
                     /locus_tag="O0394_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCY0498750.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            10624..11397
                     /locus_tag="O0394_000651"
     CDS             10624..11397
                     /locus_tag="O0394_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0498751.1"
                     /translation="MKAHKIFWLNLAAIIIISIVVSGDMFLAMKWEQIHLKDGLKKVL
                     STYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNT
                     HKITGEYDTTTNDKKNATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQS
                     GDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPKNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGKDQIIYKESLTYNKISEH"
     gene            complement(11695..13701)
                     /gene="ggt"
                     /locus_tag="O0394_000652"
     CDS             complement(11695..13701)
                     /gene="ggt"
                     /locus_tag="O0394_000652"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCY0498752.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVLEPFASGIGGGGYLLYYEQSTGSITAFDARETAPEHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLAKYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSTL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMYHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIAHGHAME
                     HKPDAYIGDVHGLQVDPTTYEASGGSDDTREGTVMGGEVLVIRKQPLPYRQMYDSDGF
                     RLYFNDVQLPLLADQVRWMHDKYWVDESVVRIIFPEVSAHIEDLRSYENAGENYIDIA
                     WLARKYAYQVTLKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR"
     gene            complement(13739..15514)
                     /locus_tag="O0394_000653"
     CDS             complement(13739..15514)
                     /locus_tag="O0394_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0498753.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSHDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            complement(15531..16694)
                     /locus_tag="O0394_000654"
     CDS             complement(15531..16694)
                     /locus_tag="O0394_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0498754.1"
                     /translation="MAQLNSKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGVCIMFVVLAFNFIGDAVRDALDPRIH"
     gene            complement(16700..18178)
                     /locus_tag="O0394_000655"
     CDS             complement(16700..18178)
                     /locus_tag="O0394_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0498755.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEI
                     EQALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENVLFHKFI
                     MIKVILALPMFILLTFYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALMVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            18135..19727
                     /locus_tag="O0394_000656"
     CDS             18135..19727
                     /locus_tag="O0394_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0498756.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLNNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVIFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQTFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            complement(19992..21104)
                     /locus_tag="O0394_000657"
     CDS             complement(19992..21104)
                     /locus_tag="O0394_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HCY0498757.1"
                     /translation="MKICEKCFNNTEIVEIIANDNSKFDNCDIDNNHLGVKIFDTTKD
                     IDKLELIRDYLRPALELYDISINLPDTFSPKEGKKIEIALKDDWSIFNVEEAQISCIL
                     NELFKDDENLDRRVLEDLVGAKIINDKKYTNKNLIVANNDWDGFCESLKYKNRFHNNM
                     INLENLAFFLDITTNYYSIEEFRERFEPLYRSRIVKFITEKSELMSPPKGFATAGRLN
                     SKWISVLYLSTKEQVSIEEVKPKHNDIIYIGKVKLQKNVTKEKLKIANLTNLTSNAIK
                     AGDDGFRKYFVNYQTLKKIHKGITNPSDEQGIDYLPFQYLADYIRSLKYDGIMYESIL
                     QDGTFNFVFFDQSLFECVNYERKRVSDVKYTLTKLS"
     gene            complement(21091..22014)
                     /locus_tag="O0394_000658"
     CDS             complement(21091..22014)
                     /locus_tag="O0394_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCY0498758.1"
                     /translation="MYYPYLRGKQNELFAIRELLEKGLIGDCIQPIIEPIKYTTTFKN
                     TLQYCGEKAFSINLVVNSKLTEEEISNETVAHLTEIITKNKSVIQKAYLGPSDEGNDR
                     LKQQFSSNSLAILTSVDDWEMFGDKNKLEMVFVPDDRHIKRKLRNIPNKGIIMDPFNK
                     LSRNVDYLDNDDEFYSDDHLYYKEDGYVAFSDYSVIGGEYVDGGFSPLAIAIHIVYFD
                     EANELRVKHFVSDSNNDRSNPGKKFFEAVDKLVTWSKNLDIKNRSYALGQFEELNENN
                     KYPGLGLIKRLSIMHHLEIMNRYLESQNENM"
     gene            complement(22016..22858)
                     /locus_tag="O0394_000659"
     CDS             complement(22016..22858)
                     /locus_tag="O0394_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HCY0498759.1"
                     /translation="MDNRNMINRVFSQKILHQIAIKNKSDVVDEAYDFYIQGPKNINV
                     IQKMKSLYNYLKKSYRNEYFYKNTILNKLLLGRHSINTTTALSEMPIGKSIADFILLN
                     GKGVVYEIKTELDKLDRLDNQINDYYEVFNYVVVITNDKHLNKVMARYKDTTVGILVL
                     TTRNTLSEVQKPKENNSLLNTKAMYNFLRKEERKRVIAQNHMDVPTYNDFTEYDVLFD
                     VFKEIPMTKLHNNMISELKKRGNMKEYKDEFLAAPAEIKFLLYFAKMTKKDKNKLYHF
                     LKED"
     gene            complement(23063..25852)
                     /locus_tag="O0394_000660"
     CDS             complement(23063..25852)
                     /locus_tag="O0394_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCY0498760.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAPLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTIAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            26065..26346
                     /locus_tag="O0394_000661"
     CDS             26065..26346
                     /locus_tag="O0394_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498761.1"
                     /translation="MVLITYQIILFFIISLSYYLTLNHYMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIIAFSTFVLVHLALKLFFSI"
     gene            complement(26540..27415)
                     /locus_tag="O0394_000662"
     CDS             complement(26540..27415)
                     /locus_tag="O0394_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0498762.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(27418..28872)
                     /locus_tag="O0394_000663"
     CDS             complement(27418..28872)
                     /locus_tag="O0394_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0498763.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            complement(28884..29780)
                     /gene="murQ"
                     /locus_tag="O0394_000664"
     CDS             complement(28884..29780)
                     /gene="murQ"
                     /locus_tag="O0394_000664"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCY0498764.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            complement(29780..30835)
                     /locus_tag="O0394_000665"
     CDS             complement(29780..30835)
                     /locus_tag="O0394_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCY0498765.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYDHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSDLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
BASE COUNT         9742 a         5961 c         4549 g        10650 t
ORIGIN      
        1 tatgttgtca aaatcaacgc ttatacatat gacataatga cggaggacat catgattcta
       61 tctatattac ttatcttttt ctgtatcaga cttgtcagct taaagatatc tattaatcac
      121 tcaaaacaat taaaagcaga cggtgcagtt gaatatggcg ttaaaaattc gaagttttta
      181 gcaataacac acgttttaat ttatgtattg gctggtgtag aggcatttat taataaagat
      241 acatttagct ttgcaaatgg tattggctta gttatattaa tctttgctta tatcatgtta
      301 tttatggtta ttaaaacttt aggtggtatt tggacattaa aattattcat tttacctaac
      361 catcctatta ttaaatctgg attatataaa attacaaaac acccaaatta cttcttaaac
      421 atcattcctg aattaatcgg tgtattatta ttaacacacg caacatatac aactatttta
      481 ttagtaccgt atgcgtattt cttatatgta cgtattaaac aagaagagaa attaatgaat
      541 atttaaaaag atgaaagcct gtcatcactt tagtctacat cgacgtccat ctttaaagtg
      601 cattctgaaa tattatattc agaaaccttt aaagtatgtc gtgtctatga ccattgtgac
      661 aacaggcttt ttttattatc atatacctca ctattgaaaa ggttatatac tcttttcaat
      721 ggtgaggatt gttactgtga atgggtatga ccatcgctat atacttatat aaagtacacc
      781 ttctttacaa acaatgactt gatttaaggt gtgaaatttt tggcaatggc ttggcgacat
      841 atgcatttat tcaacactgc caccagtctt acttcagaca aacttcagac taacctcgag
      901 ttcgtattat gcccaccaca tgtctgctgg cttgtcgtaa atattaacag atttcaaagt
      961 ttgacaagct ttttggaaac cttcttctac tgacataata ggatcttcat gttcaatact
     1021 taatacataa tcatatccat taataataag ttgacttatg atatctgccc atacatatgg
     1081 actatgtcca taaccaactg tacggaatgt ccatgctctt gtcgcaacgt taccatatgg
     1141 ttgcatatca gttagaccat acatatttac attttcttga ttaatatacg tatctttagc
     1201 atggaagtga tgaattgcat ttgcttggcc taatatgcga atcgcagcaa ttgggtcaat
     1261 accttgccac catagatgac taggatctaa gttagcaccg atatattcat ttgtagcctc
     1321 acgtaacttc aacattgtat atggtgtatg cactaaaaat cctgcatgca actctatggc
     1381 aatttttaca tcttgctctt ttgcaaactc agctaaatct tgccaatatg gtatcaactt
     1441 ttcattccac tgataatcat aaatttcaga gtaggctgtt ggccaaggtg taacaggcca
     1501 attaggcttt ttagcggtat catctgatcc tgcaatgcca gaaaatgtat taacaactgg
     1561 cacgtctaat agatttgcta aacggattgt tttacgtaac gtttcatcgg cttctttcgc
     1621 ttctatcgga tctggagaaa ttggattgtt atgacaactg aaaccactta tttgtaagcc
     1681 tctgtctgtg attgacttca taaatgcttg gcgcttgtct tcattttcta acaactcatc
     1741 taacttacaa aatttatctc ctgggttacc acctgttcca acttcaatca tatccaatcc
     1801 agattctgag acataatcta acatatcttc aaaattttta tcgtaaaata atactgaaaa
     1861 tacacctatt ttcattgaaa agcactcctt ctttttatat tgagaatttt gtatcatata
     1921 tgataatcat taaagttgta tgctcttgtt atcaagatcg ctacgataaa tcgcttctat
     1981 aagggcattt acattgcgtg cttcttccgg tttcaccaca atctcttcta tccctaaaca
     2041 cgcattgaca aagttacgcg cctgtctctc accagcaatg tcttcgttat gctcaacatt
     2101 agctttgctt tcaaaaatag ttccaaagcg gggctcatat atttcaaatg gaaataaatt
     2161 gataccgcca tcttctcctg ataaactaac gtgaacctta tcttctttga tatttgcaga
     2221 ccacgaacat tcaaactgca tgcttgctcg attggcaaat gtcatataac tagtaacatg
     2281 atcatcgaca tcaaatttag tatgatcaaa tgttccccaa tcattaattt gattcggttg
     2341 tttgctcaat tgattatatg tttttcctag cacttcatgc ggcaccatat ctttacctag
     2401 tagccacaaa gataagtcta acaagtggca accataatcg attaaactac cgccaccttg
     2461 caacgcttta ttggtaaaaa caccccagcc aggcacttta cgcctacgca tcgcttgtac
     2521 acgtgctact aaaggtttac caaccacacc tgattcaatt gcttttttag cagtaattgc
     2581 cacatctgtg tgacgataat gatatgcgac agttaataat ttgtgatttt tattagccgc
     2641 ttcaatcatg cgatcacact cttccgtcgt catcgccatt ggcttttcac acaatacatg
     2701 gacaccatgg ttcaatgctt ctatagaaag atcagcatgg aatttattag gtgtacaaat
     2761 gaccaccgca tcaacaagtt taaacagctc gctaggtgtc tcaactgcat gaggtatatt
     2821 aaagcgcttc gcaacatcaa tcatctgcac tgtattaata tcttgtactg caactaatga
     2881 gactgtgtct ttgagtttca gcaatgctgg aatatgacgg tcttgtgcaa taccaccaac
     2941 acctatcaca ccaactttta attttgtcat gatgtgcctc cttaccgtat gatgttattc
     3001 aaagtaaatt gctttgcctg attttgcaga ctgataaatc gcttcaagaa tttttgtaac
     3061 taccattgct tgttccggtt tcacaactgg ttcagtatca tttacaactg catcaatcca
     3121 agcttttgct tcttcttcag cttcatccac ttcattacct tcataaaagt cgacgccttt
     3181 gttttccaat tcaacgtgtt tggtataaag tgtacccatg tcttcaccat gaatacgtag
     3241 accatctttc atatcagcac ctgctttagt tcctgataat gaacattttg cctcatccac
     3301 ttctaaagaa ttaatcgccc aagcggattc taaaatgatc gtcgctccat tcttcatttt
     3361 aataaatcca aacgcagaat cttcaactgt aaattcatct ggattccatg aaccccaagc
     3421 gtttgccgca tgatgctgtt tatttaattt atggaatgtt gaacccatca ctgattctgg
     3481 ttcataatta tccatcatcc ataacgttaa atctaaagcg tgtgtaccga tatcgattaa
     3541 tggtcctcca ccttgagctt cttcgtctag aaagacaccc catgttggta ctgctcgacg
     3601 acgaatggca tgtgcctttc cgaagtaaat gtctcctaag tcgccacgtt gcgctgcttg
     3661 atgtaaaaat tgactatctg ctcggaaacg attttgataa cctattgtta attttttacc
     3721 tgttgattta gctgtatcta tcattttttg agcttctgct gtcgttttag ccattggttt
     3781 ttcacacatc acatgtttac cagcatgcaa ccctgctaca gtaatttcac aatgcgagtc
     3841 atttggcgta caaacatgga taacatcaat cgtgtcatct tttaacaatg ctttgtaatc
     3901 atcataaacc tttgcattgt cagttccgta tgcttctgcc gcactcgctg ctttcgaaat
     3961 gtctacgtca caaaatgcga tcatttcaac attttcaact ttttgtaaac ttggcatgtg
     4021 cttgccattc gcaataccac cacacccaat gattccaact ttaatcgtca tcataacgtc
     4081 ctccttttaa tctttatcgt cattagtggt tctttatcaa ttctgcccca ctcctaattt
     4141 caaactaacc ttttgtcgca cctgttgtta aacctgatac taaatagcgt tgcaagaaca
     4201 agaatacgat tgctataggt actgcaatca taattgcccc tgctgcaaac actgtgaaat
     4261 tatttgcata cttatcatta ataaagttga acaatccaac tgctaatgtg aatttttcag
     4321 gacttcttaa tagtatttta ggtaatataa agtccataaa tggccccata aaattgaaca
     4381 aagcaacaac tgctaaaatc ggcttagcta atggaagcat aatttgtaag aaaatacgca
     4441 tatgccctgc accatcaatt ttggcagatt catcaagttc ttttggaatc gtatcgaagt
     4501 aacctttcac taaaaaggca ttcatcggta ttgatccacc aatatatacc agtgttagtc
     4561 caaataaaga atctaataat ccaattgtat ttagcaaaat atagattgcg accattgcca
     4621 ttaatacagg gaacatttgt aaaatcaaaa atgtaatcag cccgtattta cgaccaacaa
     4681 agcgatatct agaaaaagca tatgctgtta acgtgacaaa tatcacacta aacagtgcat
     4741 ttgcagatgc tacgataagc gtatttttat accaagtcag gtattgacta ctgtcatcga
     4801 atagtaagaa tgcataattt ttaaatgttg cattgtctgg tatcattttg gcaccataca
     4861 agttcgtacc tggattaagg gaaatgccaa atgtccacag tagtggatat aaaatgatga
     4921 caaacatcat cgctataaaa ctgtaaatac cgattgcttt taatatgttt ttcttctttg
     4981 tcatcttata aacctccctc atctttaaac gtacttgtac gtctgaattg aataaatgcg
     5041 acaatagcaa caataaatcc aataattaat gacacaactg cacccatgtt gaagttgtta
     5101 aactcaaatg tcagattata cacccaagat atcaagatat ctgtactacc agcattctgc
     5161 cctgacactg gtggaccgcc tttattaaat agataaataa gattaaaatt attgaaatta
     5221 cctgcatatt gcataattaa caatggcgct gtggcgtaaa tgacatgcgg gaatgtgatg
     5281 tttctaaact tttgccaact agacgcacca tccatatctg ctgcttcgta ccaatctgat
     5341 gaaatacttt gcagtactcc agtgaacagt gcaaagacaa atgggaatcc aagccatact
     5401 tgaatgccga ttaatgccac ttttgcccaa aacggatcac ttaaccatgc tggtgctaca
     5461 cctaataaag gttgcaaaat atcattattt atcgcaccaa attcatcatt aaataacgct
     5521 acaaatatta aaattgtcac aaatgatggt acagcccaag gtaggattaa cacagtacgg
     5581 ataaatttct tacctttgac gacagggtga tttacaataa ttgccaaaaa cagccctaat
     5641 gcaatttgaa gtgtcgttgc aacaagcgtc catactaatg tccaagtaat aacactgaaa
     5701 aatgttttac gccaaacgcc aattgtgaat aacgttttaa agttatctaa accaacccat
     5761 tctaatgtgt gtctcggagg cgcgttgtat aaattgtaat ttgtaaatgc tactccaaac
     5821 ataaatatta atggaaatac aactacaaat atcaataaga atgtacctgg tgagattagt
     5881 aagtatggga acgtcttgtc ccatgttgcc accatacgcg ccttcggatc ctttatttcc
     5941 tcattgcgat taaatcgttc agcattacga tatgcatcta aaatattaat gatatatagc
     6001 atgattgcga aagcaacgag taagatagaa ataatacctt gtgcaagtaa gacacgagaa
     6061 tcgtctaact taggtactgt ccctaatgtg aacaatcccc aaaaaccaat atttaaaaag
     6121 ctataaaaaa cagaaataaa actgatgaaa aagataaaaa atatcgtccc tttaatgggt
     6181 cttttattat aaaactgtcc caaaccaggt ataacagata ataatgccgc taatttaggg
     6241 ttacgtttcg tcataactaa tctcctttct tatcattttg tgatggatga agaatcttaa
     6301 tattttgcgt tatatcattc gtcgcctcat ctaacgcttg tttaggattc ttaccatttg
     6361 aaataaatat gcttgcattg cccatcggtt cccaaacttg tcgcatttca ggaatattag
     6421 gcatcggttc agcatgacgt gcttgctttt caaacacttt taaatttgga ttagatgatt
     6481 tcacgtcaac acgtccagta atttcgctca tttcatctgt atatttttgt aatgtatctt
     6541 tactagtgat atacagcatt aaatctttag cccaatactt atgtttacta tattcagata
     6601 aataccaacc acgtacacct agaaatggtt tcataggttt gccaccatct gtaggtaatg
     6661 ttgttactcc taaatcttta ccaaacgttt cttgatattc attaatgttc cacggtccag
     6721 tgacaaattg tcctactttt ccttctttaa aaagaccaat catgacatca tgtgttgctg
     6781 ccttaggaag ataccctttg tcgtaccatt tttgtaatcg ttcagcattc ttgacgacat
     6841 gttttgaatt tagtcctagc tgatgaatat catattcact gccatttttc ttgaaaatat
     6901 aatcatcatt gccgaataaa aacggataat taaaatagaa atttttagca tcaaataaca
     6961 taccgtattg tttcttttta ctatcagtta gtttggcagc attagcttca acttcttcta
     7021 acgtttgcgg tgcatttttc actaattttt tattataaaa aagtgcggtt gtttcaacga
     7081 tagctggcaa tgctagttgc ttattgtcat aattcatcgc tttaagtgct tgcttattga
     7141 aacctttcaa ctcatctttt gataatttga tttcagcagc taagccttgt agataggcac
     7201 ttccagtatt atcatgtgct aagaaaaaga tatctggacc ttttcctgca ggagcgtcta
     7261 gcgaaatatt ttctagttga tcattttgac caatatttac aagctttact ttgatgccag
     7321 tttttttagt atattgatcc gtaatttttt tataaaacgc catttgcttg tcgccatcca
     7381 cccacatcgt aagttggtta ggcttgtctt tagaattatc tttattcaat gctttatcaa
     7441 tatcttcttt cgaacgatta gggccacatg cagttacgat taataacatt accacggcta
     7501 acgtgataca ttttaaaatt ttagacatga atactccccc ttagacgata cgatttcctg
     7561 ttttttcatc aaaaaagtga cacttattca tatcaaatgc tagtgtaatc ttgtcgttcg
     7621 ccatcacttg agttcttgaa tctaatttag aaatcaattc cattccttgg aatgtgctat
     7681 gtaccataat ttctgaacct aacagttcgg atacaactac ttcagattca aattgtgtct
     7741 ctggagaagt ttgaataaat attggttctt catgaatgtc ttcagctcga atacctaaaa
     7801 taatttcctt gtctaagtag ccagcagctt ttaacttttc aaatttttta ttatgtaatt
     7861 taaaatggtg tgttcctacc ttcaatccgt ccatttcaac tgtagcattc aacatattca
     7921 ttgctggtga gccgataaat tgcgccacaa atatgcaatt aggggcatca tatatttctc
     7981 taggtgtgcc gacttgcata atgtcgccat ctttcaaaac aacaattcga ctagccatcg
     8041 tcaatgcttc agtttgatca tgtgtaacat aaattgtcgt agtattaagt cgcttatgta
     8101 atttcaatat ttctgtgcgc atttgtactc gaagcttcgc atcaagattc gataatggtt
     8161 catccattaa aaagactttc gcatccctaa caatagctct gcccaaagca acacgttgac
     8221 gctgtccgcc agataacgct tttggtttac gaccaagata ctcagttaat cctaatattt
     8281 cagctgcttc attaactttt tgttcaatct cttttttatt tactttacgt agctttagcc
     8341 caaatgccat attttcaaaa acagtcatat gtggatataa tgcatagttt tgaaatacca
     8401 tcgcaatatc tctattcttt ggttcaacat cgttcatgcg ttccccatca atataaaaat
     8461 ctccagatgt gatagactct agtccagcaa ccattcgtaa tgttgttgat ttaccacatc
     8521 ccgatggtcc aacaaataca atgaattctt tgtcagtaat atgtagatta aaatctttca
     8581 ctacagtatt gttgttatca tacgtctttt taatatgctc taactttagt tctgccatgt
     8641 tatgcctccc atactttgtt tacagtttga ttgtgcaata cacttcgtga aaacgtttgc
     8701 acaaatactt acataaatca tatattcagt cattattaat gtcaatcata aattataaac
     8761 gtttacatta ctgtcagaat taaactttta tacagaaaat tgatatagtg cacaactatt
     8821 ttctagtgtg acaataatgt tttttatgtg cgatatttca ctgccaatgc taacaacctt
     8881 gtcttacatt ttaagtaata tctaaatcaa acaagtatga cttagcatta cgattttcat
     8941 tgaaaaaagc aaccattatg taagtatcat actcacacaa tgattgctta attttttaga
     9001 ctgcgggcac ccttctataa ctacaaatca tataggctac gttccccatc aggtaactga
     9061 tcgataaacg gttttggatc ttctgcatat gcattaccta cgccaccctt cattctttga
     9121 aaatgtaaat gtgcgcctgt cgtttgtata cctgtattgc ctgaatatgc aataatatca
     9181 cctgctttga ctcgatcacc tactttgaca ttatatttgt ctaagtgtag ataccactgg
     9241 tgatattctc cattgtcttc ggcaatctgt aataccttgc cgcccaattc attattaaag
     9301 atacgtgtta ctttaccatt cgtcggcgct ttaattggtg taccttttgg caatgcaaaa
     9361 tcaatgccat aatgcttacc atcaaatggt gaatgttgat atttaccaaa agtctcagta
     9421 atccgactcc ctctaaaaaa gtcttcccat tttgagtgtc cttgtccttt atgattttgt
     9481 gtcaatttat cggtatattg gttaaaccat ttttgaatgt catctatata aataatcgta
     9541 aataatagtg aaagaaaaat gataattgtt gccaaaatac gttttggtcg ctttgtcata
     9601 gtggcatcca tccttcatat attttgggta ttaagtatat attctaaatc aactatttca
     9661 taattaaatg cactcatact ttagtagtac tgccaaatat agatgaagaa aatgatggta
     9721 atttgaatat tttgattgat attaaatttt atatcgagta taataattat acttaagaaa
     9781 ataacagaaa cagaggagaa ttttcaaatg gcaaaagtat tatatattac agcacaccct
     9841 tttaatgaac tggtatcaaa ttctatggcg gctggtaaag catttatcga aacatatcaa
     9901 caacaacatc ctgatgatga agtgaaacat attgatttat ttgaaactta tattccagtt
     9961 attgacaaag atgtattaac tggttgggga aaaatgagta atggcgaaac gttaactgat
    10021 gatgaacaaa tgaaagtttc aagattgagt gacattttag aagaattttt aagtgcagat
    10081 aaatatgtat ttgtaacgcc tatgtggaac ctttctttcc cacctgtagt taaagcatat
    10141 attgatgcta tttcaattgc aggtaaaact tttaaatatt ctgctgaagg accacaaggt
    10201 ttactaactg acaaaaaagt attacacatt caatcacgtg gtggatatta tactgaagga
    10261 cctgctgctg attttgaaat gggtgaccgt tatttaagaa caattatgac attcttaggt
    10321 gtgccatcat atgaaacaat cattattgaa ggacacaatg ctgaacctca taagactgaa
    10381 gaaattaaag caactagtat taataatgct gaaaaattag caacaacttt ttaaattttt
    10441 agttacactg tgtgacgtct caaacgtcat gcagtttttt attgtatctc ttttccgctt
    10501 tttataaata tctattattt tcataaattt aaaatttgat attcacttta atactgatgt
    10561 aaatattttt caaaatatat tattatagtg ttaatgaagt attttgagaa acggtgggaa
    10621 tgtatgaaag cacataaaat tttctggtta aacttagcag caattattat tatttcaatt
    10681 gtagtcagtg gcgatatgtt tttggcaatg aagtgggaac aaattcattt gaaagatggt
    10741 cttaaaaaag tactaagtac gtatcctatt aaaaacttag aaacgcttta tgaaattgat
    10801 ggtcacgata atccccatta tgaaaataat gatcaagaca catggtatat agaatcctct
    10861 tattcagttg ttggatcaga tgagctttta aaagaagacc gtatgttgtt aaaggtagat
    10921 aagaacacac ataaaataac tggtgaatat gacacaacta caaacgataa aaaaaatgct
    10981 actgactcga catacaaaag ttatccagta aaagtagtta ataataaaat cgttttcaca
    11041 aaagacgtaa aagatcccgc gctgaaacaa aaaatcgaaa acaatcaatt tttgattcaa
    11101 agtggtgatt taacaagcat tttaaatagc aatgatttga aagtcacaca tgatcctacc
    11161 actgattatt ataatttatc tggtaagttg tcgaacgata atccaaacgt taaacaatta
    11221 aaacgtagat ataatattcc taaaaacgca tcaacaaagg tggaattaaa gggaatgagt
    11281 gatttaaaag gcaataatca tcaagatcag aaactttatt tttatttttc aagtcctgga
    11341 aaagaccaaa tcatttataa agaaagcctt acttataata aaataagtga acattaatac
    11401 ttatgctgta attatagaaa catccaaatc atctattaaa atcctatatt ataaaaacac
    11461 ctcacataac tcgttcaact gtaccaaacc acattacatt agattttagg ctaactattg
    11521 tgatgtacat caaaaacgaa tttgtgaggc gttgtatatt ttacaaaggt gactagcgtt
    11581 tcgtatagca tttccaacat tactacactc aagcgtcacg ctaaagttcg aaatcgaatc
    11641 ctttcattca acaaaagctc atatccacta caaacttcat atcaagcgta taaactatct
    11701 tgtgatacta tctcgatcat atctatagta tgcatttgtg ttccgtttca ctgaagtata
    11761 tgtatcatca gttaagtata aaccgtcatc cttcaatgtt acttgataag catatttccg
    11821 tgctaaccag gcaatatcta tataattttc tcctgcgttt tcataacttc ttaaatcttc
    11881 aatatgtgca ctaacttcag ggaaaatgat tctaacaaca ctttcatcaa cccaatattt
    11941 gtcatgcatc catcgcactt gatctgccaa taaaggtaac tgcacatcat tgaaatatag
    12001 acgaaagccg tcactatcat acatttgccg atatggtaat ggctgttttc taatcactaa
    12061 cacctcgcca cccattacgg tgccttctct agtatcatca cttccacccg aagcttcata
    12121 cgttgttggg tcaacctgta gtccatgtac atctccaata taagcatctg gtttatgttc
    12181 cattgcatgt ccatgtgcaa tcaatgctaa tattgtagat tgtgaaaatt gaggctccca
    12241 ttcaatgcga ttaggatggc tactataaat tctaggttca tctatagcct gctgaatatc
    12301 catgccaaac actaatacat tgattaatgt ttgcgcaaca ctagcaatga tacttatggc
    12361 accaggtgca cctactgtta atattggctt cccgtgatac atcacaatcg ttggagccat
    12421 gttacttagt ggtcgtttat atggtgcaat ttcgttaata ccaccatcta ctacatcaaa
    12481 gccatccatt gtcgtattca ataacacacc gtagcctgga atcgtgatac ctgaaccata
    12541 aatcatacca attgatgtcg taaatgaagc aatattacct tccttatcaa tcacacaaaa
    12601 atgagttgtt tcggtatgat tttcttcaac atctgtatga ctaatgcaat cagacacaac
    12661 accatgctct atatcaatat ttgctttatt gctatcaatg agcgtactgc gtgcttttaa
    12721 ataatcatca tcaattaatg actgtacagg cacctcatga aaattatcat ccgccaagta
    12781 ttgcgcacga tcactatatg ctaaatgcat cgcttgtatc aaatgatgca agtaatcaac
    12841 agatcttgga cccatagatg gtaaatcgac atgttctaat aacttcaata tttgaattac
    12901 cgtgataccg ccagaactag atggtcccat tgaataaatg tcatagtctt taaatgttgc
    12961 actgattggc gctttaatct gaatgtcata tttggctaga tcctctaaag tgattgtccc
    13021 accacatgct ttgacaacat tgactaattg tttcgcaatg tcacctttat aaaatgcatt
    13081 aaacccttgt tctcttaata tttgaaatgt cttacctaat tcgggttgta caatccaatc
    13141 accttcacgc caatattgat tttcatgcgt aaatacttgt gccgtttcat gatactttgt
    13201 caatcgtgcg tgttgctggc gcgaatattt ttcagtagcc caattggctg catgaccttc
    13261 aatggctagt tcaattgcag gattaattaa atcttccaat gacaatttag cataacgctt
    13321 gtgaatataa tcaaacagct ttggaattgc tggcacagcg acagttttac catgtgtagt
    13381 catatcaaaa aatgatttat attcgcctga atcatctaga taaaattgtt tgtctacatg
    13441 ttcaggtgct gtctcacgtg catcaaacgc agttatactg ccagtacttt gctcataata
    13501 tagcaaatac ccgccaccac caatacctga tgcaaatggt tctaacacat tcaatgccag
    13561 ttgaattgca atcactgcat ccatggcgtt gccaccttga tctaatacat ccttaccaat
    13621 tttagccgca agaggatgtg atacggaaat taacccttct ttagatgttt ttgtctgttt
    13681 gtcatttaag ttaatgacca tactatatcc tcctactttc tgttaaatat ttaaaacatt
    13741 attgattaat ggctttttct actttttcta aatcttgacg ttgctcgtta ccagtatcga
    13801 caagtggtgt aatcggtgat gcaattttaa atttatcgcc acgataaaac ttaataaatt
    13861 gatcctgatc tatcgcatta actactgctt gtctcaagtt tggatgcgtc ttaaatatac
    13921 cttttttaat atttagcatt aaaaagactg acttgcgtcc atttttgcga ataatgctta
    13981 aatttttatc cgacttaatt aaatcaaaat gtttttgatt cacatctgcc aacatatcaa
    14041 ttgaatgatt tctaagttct gacaatgcat tattcgggtc accattaaac ttcaatgtaa
    14101 tatttttaat tttagctggt ccataactac ctttttctgt ttcgttgaat cctggattac
    14161 gttgaaacgt tgcttgatat gcatttttct gtgtcataat gtatgcgcca cttgcataca
    14221 gcgcattttt cccatctgaa tttgcaggaa ttgtactgct atccccatat ccttttggat
    14281 attcttgatt tacttgatta acaaattttt tagataaaat gcctgccgaa gagtgtgtta
    14341 agtaatttac ctctcgaggc atcgattgat ctgtcgtaat tttaacaatt tgataaatac
    14401 cgtctttatt atttactttt tgaccatctg tcgttaacga tttgacgtta taagctttaa
    14461 tcaacttatc atagattgat ttatcgtcct tgtctttctc tttacgcaac tgatcgatgt
    14521 cctcatcttt taatatcttg atgtcattta tatgtttgtg catattgtaa gtattattgt
    14581 taggcacaga ctttttatca cgtgctctat ctaaagaaaa cttaacatct tcagccgata
    14641 cacgctctcc agtattacgt gcttgtccat tgaccacttt cgcaaaataa tcatcatctc
    14701 ttaacaagaa ataaaatgct ttattgtcct tattcacagc ataatcatga cttaacgaac
    14761 ctttcgttgt taaatgatca ttttcatcta ataataataa ccttgtgtac atattcatat
    14821 taattgaata tactgacggc gcaattgaac gtattggatc caatgtagga atttcaccat
    14881 cttgttgtgt catcacaagt ggccgcgtat ctcgttctct actattgttg taatcaaatt
    14941 gttgccatat taatgcacgt gaatttggca atccaacact atttttatct aacactttat
    15001 tgtcatatac taaattcttt tttgatccat ataaaggcgc catataccct ttatcaaata
    15061 caacttcatc ttcaatttgc ttatatgttt gtttaacatc tgcttcattt tgagtagaag
    15121 ctttatttaa caactggtct acatgtttat ctttcaataa actatttgat cctgtagaac
    15181 taaataatgc cgtcatagca tagttcgggt caccaaacac tgtcatccag tcatcaattt
    15241 ggatatcata attgccggct tgacgttgtg tacgatagct accataatct ggttggatat
    15301 tcatcttcac gttaaatcct gcattttcca attgatcttt aacgatattc atatcatttt
    15361 cataacttgc ttgtcctagg aaatgtattg ttggtcgctc gcctttcact tcaactttcg
    15421 atgacttttg agccacttct gatttcgtag ggacaccaca accacttaat accaacgcta
    15481 aaactataat tgcgatacta atgattttct tcacatctat ccctaccttt ttaatgaatt
    15541 cttggatcta gtgcatcacg cactgcatca cctataaaat taaatgctaa aacgacgaac
    15601 ataatacaaa caccaggtac aatagctaaa ttactgtgcg tttccaagta gttactaccg
    15661 gtacgtaaaa tgttgcccca ttcagctaca tcaggtgcaa caccaagtcc taggaaactt
    15721 aaactacttg ttgttaatac aaccacacct atatttaatg aaaaacgtac aatcataggc
    15781 gcaatcgcat tcggtaaaat ataacgccat atgatattcc aagtgttttc accagtgata
    15841 cgtgctgcat ctacatattc catgcgttta atttctaaaa cactggcacg cattgtccgt
    15901 gcaaatgatg gtatattacc gatacttaaa gcaataatta aatttggaat acttgctcca
    15961 aatgatgcaa taattgccac cgctaacaat aatgatggaa ttgcaaacac tacatctaaa
    16021 attcgcatta ttaaattatc aatatgatta aaataacctg cgatagtgcc tagtaacaca
    16081 ccaaaaataa ctgcaataac tactgaaata attgaaattg aaaatgtcag cttcgttcct
    16141 acaactacgc gtgtaaataa gtctctaccg aaatcatcag taccaaacgg ataggctaga
    16201 ctcggtccat gtaacagtgc attgaactga tttttagtag ccaatgtcgt atcaaatgta
    16261 aattgtgaca caattgataa tgtcagcatg tagactaaaa taagtaaccc gataatcgca
    16321 atacgatgtc tagtagtttt tcgtataaac gattcccacc cgttataact atgtatttgc
    16381 gatgtacgtt ggtaacgtct aatacttaca aacattaata atgtaaatac gttgcctgtt
    16441 aatgtcatca acaataacaa cacttcgacg atacgtcgcc ataggtcatg atgcttccat
    16501 gtttgttccg ttgttaaaat aataattaaa atgatggtta aaacgattag caatgtttca
    16561 gcaatataga acgtatcggc cacataacct ttaaaaagat ttaatgcact cgttaatata
    16621 actaaaatat aagttgctat ggcgtaactt gcgaataatt ttaaggaagc tatctttgaa
    16681 ttaagttgtg ccatatgcct cacttccttt cgttgatttc actacgtaat tttggatcga
    16741 ttaaagcata aaatatatca ataattaagt ttgctaaaga tattacaatt gatatatata
    16801 cgaccccacc catgactgct ggaatatcag gtattagttg tttttggacg atataacgcc
    16861 cgataccatt aatgttaaat acttgttccg tcactgctga accgcctagt aactctgcca
    16921 ctagaagacc aactaacgtt acaattggaa taatggcatt tttcaaaata tgtttaataa
    16981 caacttgtgt cgtcgataat ccttttgcat aagcagttaa aacataatcg ctgcgcatta
    17041 cttcaagtac agaagacctt gtcatacgcg tgatagaagc agcaatactt gttccaatga
    17101 caagtacagg taaaatcaac gatattggat gttctggcat ataagatggt ggcaaaatat
    17161 ccaatttcaa tgagaacgct aaaatgaata atagcccttg ccagaaactt ggaatagata
    17221 aaccaattaa tgcaattatc attaacgtga tatcaagcca actatttcgc ttcatcgcac
    17281 tgataatacc aattggtatt gcaataatta atgccaccat tagcgctaat actgcgacaa
    17341 ttattgtaat tggaattctt tcgccaactg ctttagtcac aacctcattc cctttgtaag
    17401 tcgtacctaa gtcaaaggta aaaacaccct tgatggtatc ccacaattga ataaaataag
    17461 gttcgttaag atgatgtaat acattgaatt gatgtatctg tgcctttgtt gcattttgtc
    17521 ccagtatgct ataagccgca tcaagcggtg aaaaatacag aatggtaaac acactgacaa
    17581 taacaccaat gatgacaatc acagccatga caattcgttc aaaaatatat ctaactaatg
    17641 gctgtaaata aaaagtcaat aagatgaaca tcggcaaggc caatatcact ttgatcatga
    17701 tgaacttatg aaataataca ttttcaaagt atgttgaaaa atgtgcttgt tcaatattct
    17761 ttgaactcgt attagaactt tgtgccttga atatttttaa tgcttcttta tgtatttgtg
    17821 tggatgactt ttgctgcgat aaatatttat atttttgatg taacgcctgt tcaatttctg
    17881 aaatttcaga attattagcg taaaaatttt tcctcttagc agaaaagaaa aactttatca
    17941 ctgcatataa aaatattggc aagcttaata ccgataatac aaacttgtaa aatgccttgt
    18001 gatacaacga cttaaaagat tctaaataag taattatgat aatcactcct tgtaaaataa
    18061 tcttgagtag attactatga tatacaaaag tatagaataa atttacacat ttgtgaatag
    18121 ggaggcacaa catcatgtca aatttattag aagtcaacag tctgaatgta caattcaatt
    18181 atgatgaaac tacagttcaa gcggtaaaaa acgtctcttt cgaattacga aaaaaacata
    18241 tcctaggtat tgttggtgaa tcaggatcag gaaaaagtat taccgctaaa tctattttag
    18301 ggctactacc agattatcca gatcacacat taacaggaga aattattttt aatgggcaat
    18361 cgttaaataa tttatcaact tcagcgttac aacaaattcg aggtaaggat atttcaatga
    18421 tttttcaaga tccactctct tcgttgaatc caagattaac gattggcaaa caaattacag
    18481 aagtaatatt tcaacataaa cgtgtatcta aatctgaagc aaagtcgatg acaatagaca
    18541 ttttagaaaa agtaggtata aaacatgcaa ctcgacaatt tgatgcttat ccacatgaac
    18601 tttctggtgg tatgcgtcaa cgtgtcatga tagcaatggc attgatttta aagccacaaa
    18661 ttttaatcgc agatgaacca acaacggcat tagatgccag tacacaaaat caattactgc
    18721 agttaatgaa gtccctttat gagtacacag aaacatctat tatttttatc actcacgatt
    18781 taggcgctgt gtatcaattt tgcgacgatg tgattgtaat gaaagatgga agtgtcgttg
    18841 aaagtggcac ggttgaaagt atttttaaat cgccacaaca tacctataca aaacgcttaa
    18901 tagatgcgat tcctgatatt catcaaacgc gtccgccaag accgttaaac aatgatattt
    18961 tattaaaatt cgatcgcgtg agcgtggatt acacatcacc gagtggcagc ctataccgag
    19021 cagttaatga tattaacttg gctattagaa aaggcgaaac attaggcatt gtcggtgaat
    19081 cagggtcagg gaaatcgaca ttagctaaga cggtcgtcgg tctaaaggaa gtgtcagaag
    19141 gctttatttg gtataacgaa ttaccattaa gtttatttaa agatgatgaa ttgaaatctt
    19201 tacgacaaga gatacaaatg atttttcaag atccattcgc atctattaat ccaagattta
    19261 aagtcattga tgtgattaaa cgaccactaa tcattcatgg gaaagtcaaa gataatgatg
    19321 acattattaa aactgtcgta tcgttgttag aaaaggttgg cctagatcaa actttcttat
    19381 atcgctatcc acacgaatta tctggtgggc aacgtcagcg tgtaagtatc gcgagagcac
    19441 ttgctgttga acctaaagtg attgtttgcg acgaggcagt gtccgcttta gacgtttcaa
    19501 ttcaaaaaga tatcatcgag ttattaaaac aattacagtt agacttcggc atcacttatt
    19561 tattcatcac acatgacatg ggtgttatca atgaaatatg tgatcgcgtt gcagttatga
    19621 aaaatggcga aatcgttgaa ctgaataaca cagaagatat tatcaaacat ccgcagtcag
    19681 actatgcaaa gcaacttatt tcagaagtag cagttattgc taaataaaag tcatgcgttg
    19741 tgcaacttta tcactgtatg gtctgaaata aattgcgcga cttctgatgt gttatgttag
    19801 atgactttgt catactaact atattgtgct aagttagggc tcggcaccat atcaagtttt
    19861 aggtgctttg ccatgattta agagtcaccc ccatactttg ggcattttaa cgccagaata
    19921 aatcccccgc cactatgtga agtgcggggg attatttata ttttattaga atattcagat
    19981 ttttgagtgt gtcaacttag cttagtcaat gtatatttaa cgtcacttac tctttttctt
    20041 tcataattaa cacattcaaa taaactttga tcaaaaaaca caaagttaaa agtaccatct
    20101 tgtaatatgc tctcatacat tatcccgtca tatttaaggc ttcgaatata atcagctaaa
    20161 tattgaaatg gcaaataatc tattccttgt tcatcgcttg gatttgttat tcctttatga
    20221 atctttttta atgtttggta atttacaaaa tactttctaa atccatcatc gccagctttg
    20281 attgcattac tagttaaatt agttaaattc gcaattttca atttctcttt tgtcacgttt
    20341 ttttgtaact taaccttacc tatataaata atgtcattat gcttaggttt aacttcttct
    20401 atactgacct gttcttttgt actaaggtat aatacgctta tccatttaga attcaatctt
    20461 cctgccgttg caaatccctt tggtggtgac attagttcac ttttctctgt aatgaactta
    20521 actattctag atctatataa tggttcaaat ctttctctaa attcctcaat actatagtaa
    20581 ttagtagtga tatcgagaaa gaacgctaaa ttctctaaat tgatcatatt attatgaaat
    20641 ctatttttat acttcaagct ctcacaaaat ccatcccagt cattatttgc tacaattaga
    20701 tttttatttg tatatttttt atcgtttatg attttagcgc ctactaaatc ttccaacact
    20761 cgtctatcta aattttcatc atctttaaaa agttcattta aaatacaact tatttgagct
    20821 tcctcaacat taaatatact ccagtcgtct tttaatgcta tttcaatctt tttaccttct
    20881 tttgggctaa aagtatctgg taaatttata ctaatatcat ataattctaa tgctggtctt
    20941 aaataatctc taataagttc taatttatct atgtccttag tcgtatcaaa tattttaaca
    21001 ccaagatgat tgttatcaat atcacaattg tcaaatttgc tattatcatt tgcaatgatt
    21061 tctacgattt cagtattatt aaaacatttt tcacatattt tcattttgag actccaagta
    21121 tctattcata atttctaggt gatgcatgat agataacctt ttaattaaac ctaatcctgg
    21181 atacttatta ttttcattta attcttcaaa ttgtccaagc gcataagatc tatttttaat
    21241 atctaagttt tttgaccatg ttactaattt atctacagcc tcaaaaaact ttttacctgg
    21301 atttgatcta tcattattag aatcagagac aaaatgctta actcttagct cattagcctc
    21361 atcaaaatag acaatatgta tcgcaatagc taatggcgaa aagccaccgt ctacatattc
    21421 tccacctata acagaatagt ctgaaaatgc tacgtatcca tcttccttat aataaaggtg
    21481 atcgtcgcta taaaactcgt catcattatc taaataatca acattacgac ttagtttatt
    21541 aaaaggatcc ataatgatgc ctttgtttgg aatattacgc aatttacgtt taatgtgtct
    21601 atcatctggt acaaaaacca tttcaagttt atttttatct ccaaacattt cccaatcatc
    21661 tacacttgtt aaaatagcta aactattact tgaaaattgc tgtttcaacc tatcattgcc
    21721 ttcatcagaa ggacccaagt aagctttttg aataacactt ttgttttttg ttattatttc
    21781 agttaaatgt gcaacagttt cgttactaat ctcttcttca gttaactttg aatttactac
    21841 taaatttata gagaatgctt tttcaccaca gtattgcaaa gtatttttaa atgtggttgt
    21901 atatttaatt ggttcaatta taggttgaat acaatcacca atcaaacctt tctctaacaa
    21961 ttctctaatt gcaaatagtt cattttgttt cccacgcaaa taaggataat acatattaat
    22021 cctccttaag aaaatgatat agtttatttt tatctttctt tgtcattttt gcgaaatata
    22081 acaagaactt aatttcagcc ggcgctgcta aaaattcatc tttgtattct ttcatgtttc
    22141 ctcttttttt caactcagaa atcatattgt tatgcagttt cgtcattggt atttctttaa
    22201 acacgtcaaa taacacatca tactctgtga aatcattata agttggcaca tccatatgat
    22261 tttgtgcaat aactcttttt ctttcttctt ttcgtaaaaa gttgtacatc gcttttgtgt
    22321 ttaagagact attgttttct ttaggttttt gaacttcact cagtgtattt ctagttgtta
    22381 acactaaaat tccaactgtt gtatctttgt atctagccat aactttattc agatgtttgt
    22441 catttgtaat aactacgaca taattaaaca cttcataata atcattaatt tgattatcta
    22501 atctatccag cttatctaat tctgttttaa tctcatagac aacgcctttg ccgtttaaca
    22561 atataaaatc agcaatactt ttccctatgg gcatctcaga aagtgcagta gttgtattaa
    22621 tagaatgtcg tcctagaagg agtttattaa gtattgtgtt tttgtaaaaa tattcgttac
    22681 gataagactt tttaagatag ttatataaag atttcatctt ctgtattaca ttgatatttt
    22741 taggtccctg tatataaaaa tcatatgcct catcaacaac atcactttta tttttgattg
    22801 caatttgatg taatatcttt tgactaaaaa cacgattaat catatttcta ttatccatag
    22861 ctacgcacct ccttcaacat acatttaata cttatcctgt taatattata aactatttta
    22921 aatgcctata gacattattt ctatacacat acgattataa aattgtattg aaaccaaatg
    22981 atggttgcca aacacatggt aactaacaaa agatagaaaa taataatgcc ccctacacta
    23041 atctagcgag ggggctgaat cattacacac cgtatttttc agttgtttct gccacgaaag
    23101 attcaatggc ttttcttgct ttacgctttt ctttcaatgg caaatcacca atcatttttt
    23161 taaggtgatg tgggtttaca acaccactat actggtagtc atttgaaatt gtttttaggg
    23221 cttgttcatc gatagatctc tctccagcaa atcctttgaa ctccgcttct tttttaatac
    23281 tttcgaaatt aacatattct tgatcgatat catcatcctt atttaaagaa ggtacaacat
    23341 tgtcgatgaa ttctctaatt agatctcgtt ttaacctcaa tgtcggatca tctgcatgat
    23401 ctaaaatgcg tctaatttgt tcttggttac gacgttgttc cgctttgtct tcaagatcaa
    23461 tttgtctcaa tatattcata atataattca cattaatcgt atcattacgc atcatttcta
    23521 tttcgaaatc aatatcattt aaaatggata ctttattttt ctcagccgtc gctcttttta
    23581 cttgatcgta cacagctaaa tatttacttt tatagtcttc attctcttgt tcatccattc
    23641 caatttcatc aattgtaaac tcaaactcgt caaatgcttt taaacgtaat attattttag
    23701 ctaataaacg ataagcttca acaaagcgct ttagctcttc ttcatcttga atgtcatcaa
    23761 ccatgtgtgg tgtcggcaca atcattttaa gctcacgata agcgtccata aattcttttt
    23821 tatactcttc ataactgcgc attaaaattg tatccgtatc atttgtttgt gagaatactc
    23881 tcagtgcatc gtctgtctct tttttcaagt cacgatagtt tacaatttta ccaaatggct
    23941 ttgattcttt ttcaacccta tttgtacgtg aatacgcttg aattaaatca tgatacatta
    24001 aattcttatc aacatataaa gtgttcagta ctttactatc aaaaccagtt aagaacatat
    24061 taacaacgat taagatatca attttactat ctttaacgcc ctttttaacg ttttttgaaa
    24121 tatgattaaa atactcatta gttgtgtctg ttgaaaaatt cgtctcgaac tttttattat
    24181 aatcactaat cattatctct aatttttcac gtgaatgata tggcacttca ccatcacgat
    24241 catcttcatt aggtttaaac gtaaatatac cagctatcgt taacggttgt tccaactttt
    24301 tgttaagtcg cttaaatgtc tcataatatt taataagcgc gtgaatactt tggactgtaa
    24361 atatacttga atattgacga ttacgtgtat atttatcatg attattgatg atatgtcgtg
    24421 ttactaattc cacacgttta tccgctaacc atacttcttc cgtatcaatt gcttcaacca
    24481 tgctgttatc ttctgctttt aaagctttat ttttaaaagt attaatatag tcaactgaga
    24541 aaccaagtac attaccatca tgaatggcat ctctaattaa atacgtatgt aagcatctac
    24601 cgaaaatatc tgcagttgtt ctaccatctt gactactatt ttctggaaaa cgtggcgtac
    24661 cagtgaatcc aaagtattgg gcatttttga aatgttgttt aactagacga tgcatgtcac
    24721 caaattgact gcgatgacac tcatcaataa taaatacaac tttattcgtt ttatactgtt
    24781 ctaataaagg ggcattccct tgaatcgctt tagccatttt ttgaatcgtc gttacaataa
    24841 gtggcaaact tttatcattt agttggcgta ccagttgcga ggtattaaaa gttttgtcta
    24901 cagcaccctt agcaaattta ttaaattcct cttctgtttg actatccaag tctttacggt
    24961 caaccaaaaa gataactttc ttaatgtcat cttgctgtga taaaatctga ctcgctttaa
    25021 aagaagtcaa cgtcttacca cttccagttg tatgccatac atatccatta ttccctgtct
    25081 cagtcgcttg ttgaataagt gcttctaccg catacacttg atacggacgc attgccatca
    25141 gtattctatc tgtttcatta ataatcatat agcgcgatat catcttagct aattgacaag
    25201 gtctcataaa tgactcagca aacgattgca atgtattgat acggttattc tgtttatcac
    25261 tccaataaaa catgtgactc ttcaatagtt cgctatcatt attagaaaag tatcgcgttt
    25321 caacaccatt actaatgata aacatttgta tgtagcggaa taagcctgtg taattttgtt
    25381 tgcggtaacg ttttacttgg ttaaacgcct cattaatatc aatacctcga cgtttcaatt
    25441 caacttggac aaggggtagt ccgttgatta atatcgttac atcataacgt gctttatatg
    25501 tatcctcgac agatacttga ttcgtcactt gaaacttatt tttacaccaa cttttcgtat
    25561 ctaaaaacga caaataaatc tcagactcat catcacgtct aagtggtaat ttatcacgta
    25621 aaatacgggc actctcgaaa atactttttc catcaatcat cgttaacaga cgttgaaatt
    25681 ctttatctgt taagggattg ccttctaatt tgtccgcatg acgctcattt aaaatcgttc
    25741 taaaattatc aagcaattgc ttattatcac gtatcgttac tctttcgtaa cccaattgtt
    25801 caagttgatt catcatttca ttttctaatg cgtattcact ttggtatgcc attcatatcc
    25861 ccttccatac actttctatt gctctaaata tatcataaac tttaatgaaa aatgtttgtt
    25921 ttttatcttc aaacgtaaat ttattctaat tttattgtct tatcttttaa tatttgtctt
    25981 tgaggtaagt cgtatactaa aatttgaata caaataatca aatcattgat aaattttttg
    26041 tctacgatta atggagggac ttgaatggtg ttaattacct atcaaatcat tttatttttt
    26101 attattagtc taagttacta tttaacttta aatcattaca tggcagtcac tgtaggtaac
    26161 ttcacttcaa tattcggcat gttcgcagcc atactcttta tgtactacta cctactctat
    26221 aaaagtcccg aatacaatca acgcaaacga tttaaacatt tcattcatat cactaatttg
    26281 ataataattg cttttagcac cttcgtatta gttcatttag cattaaaatt attcttcagc
    26341 atttaatttc catctatgaa aaaagcaaag ctcaaatctg aactttgctt taatttgtca
    26401 cgcctttatc attttcaaaa tagcctctat gccagtttta caaacttgta gcaacaattt
    26461 ttcatcaagc aactgaatca catcaaaaac ttcaattgtt tacgttgcaa ctaactttat
    26521 gttagaacgc acacaatatc taatgtttaa aatctatcgt cgcaagatgc ttcctgtaat
    26581 tatcaagtgc cattttcgat tgggttatac aatctagaat cgcatgataa tttaatgcta
    26641 caaatcgata gtacaatata tctaccgtga ataactgtgc aaatagtgac gttgtagccg
    26701 ccatacgcat ttcattttca tcagttctgc cataaatcaa tgcatagtct gcaatttgag
    26761 ccactggatt attagctgta ctagatatag ttatgatggg aatactgtaa tgtgtggcca
    26821 cctgtgcaat tgactgcaat tcactatgac taccttgatt cgtcacaaaa atcatgcaat
    26881 ctctatcatc atgcgtcgca aatgttgaca caagtaaatg cgtttcatgt aataacctga
    26941 catttaagcc aatacgagat aacttttgaa aaagatcacc aatagtcaaa ctcgatgcgc
    27001 caaatccaaa taaaaatatt gtcctggcat ttttcaacac atcacaaatt gcatcaattt
    27061 gcgcatccat aatattagta gctacaaatc gcatcgtatt cgttgctcta gcaatcattt
    27121 tatttttcaa agtttctaca gattcatttt caatcaattc taaatgtgga ttggttgcaa
    27181 tatcttcggg taagtatcga gatatcgcaa tctttagctc ttgaaaacct tgatgtgtca
    27241 ttttccgact aaatctaaca attgatgctg tactaacatt cgtaacatct gccaaatcat
    27301 tcacagtcat atcaatgatt ttatgtggat tctttaaaat gtaatcagcg attatctttt
    27361 ctgtcttcgt aaaatcactc aactgcttat caatgcgata taaaatattt gtcatcatta
    27421 atcacccaac aaatctgtct gtcgcatcgc ctttgtcgtt ccaaataaat atgtacaaac
    27481 gaatccacca gcatacgcag caagtaatcc tgcaatataa cctaaataca tattatctga
    27541 gattaatggt aatagtgaca caccacttgg gcctattgct ttggcaccaa tatgtccaat
    27601 tccacctatt acagcgccac caataccacc accaatacaa gcagttaaga aaggtcgacc
    27661 taatggcaaa gtcacaccat agattaatgg ttctccgata cctaggaaac caactggcaa
    27721 tgcacctttt aaagtattac gtaatgttgt gttgcgttta catcttaccc aaagtgctaa
    27781 tgcggcacct acttgtccag caccagccat cgctgcaatt ggcaataagt aagtagcacc
    27841 tgattggtta atcatttcta tatgaattgg cgtaaaaata tgatgaagcc ctaacataac
    27901 taacggtagg aagcttgcac caatgataaa tccactaaat acgccaccaa tactaataat
    27961 tccgttaact actgaaacta aactgtctga aacaaaacct gctaatggca taaagataaa
    28021 gatagttaat agtcctacaa tcaacaatgc aatagtcggc gttacaataa tatcaatcgc
    28081 atttggcaca attttatgta atctcttttc gacaatactt aaaatccaaa cggcaaaaat
    28141 aacgccaata atcccacctt gtccaggttg caatggttct ccagtgaaga cattcattaa
    28201 aatattttta ccagcaatac ccgttaataa cgttgtacca ccaatcacgc caccaagtcc
    28261 tggtgtcgca ccaaattctt tagccgcatt aataccagtg aaaatagcta agtatgctaa
    28321 cataccgtct ttaatgacat taaatactgt tataagttgc gtaatccaag cacctgaaat
    28381 atagcctgcc accattaagt tactcagtac tgctgcaata ccaccaatta atccagctcc
    28441 aataaatgca ggaatcaacg gtataaagat attggcaatt gatttcaata ctttattcaa
    28501 cttaccattc ttttgttttg ctttatgcgc ttccttattc gcctttgctt tatcagctgc
    28561 atatgattta tagtccattt tttcactatc attgtgatgg tgtggtattg ggtcacctag
    28621 tttaacacca cttaattccg ccatatgatt agccacttta ttgactgtac caggtccaac
    28681 cacaacttga atgcgttcat cgtgtataac acccatgaca ccatcaatat gccttagttc
    28741 ttggtcatct actttattct catctaatac tttaatacgc acacgtgtca tacagttcat
    28801 gacactatct atattatcca taccacctac tgcagcaata attcgttctg caagttgttg
    28861 ttctttggtc atttaaatcc ctcctaaggt tgtctatctc tgattgctcg tttaacaatg
    28921 tcaccattgt ttaataaccg tcttgttgct tcttccttag aaatgccaca catacccata
    28981 actgtcgcaa ctttcacatc atgctcagat acctgatata acgccattgc ttcatcatat
    29041 gtgatagcac atatttcttg aataatacgc actgaacggt cgatcagttt ttgattggtt
    29101 gctttaacat caatcatgag gttatcgtaa acttttccga caccaaccat tgtgatggtt
    29161 gaaatcatat ttaaaattaa cttttgtgct gtaccagact ttaaacgtgt tgaaccagtt
    29221 aatacttctg gaccaacttt aacttctact ggatactgcg caatttcact tataactgca
    29281 tgttcattgc atgaaataga tactgttgta gcaccgattg tgttagcaaa tgttaaaccg
    29341 cctataacat atggcgtttt gccactcgcg gcaattccta taacgacatc ttttgatgtt
    29401 aaatctatat ttttcaaatc ttcttccgct aattttttgt gatcttccgc accttctaca
    29461 gccatcgtca tagcatgttg tccaccagca ataataccta taatttcatg agggtcagta
    29521 ttgaatgtag gtacacactc cgctgcatct aagacaccca accttccact tgtacctgca
    29581 ccgatataaa tcaatcgtcc acccttttta tactgtgcaa ttgttttttt aattactttt
    29641 gtcaattgtg gtattgcctt tcgaactgct aacgggactt gctgatcttc tttattcatc
    29701 gtaattaaag cctcttccac agtcatttca tcaagatgca tcgtcgcttc attacgcgct
    29761 tcggtcgtac tattttccat cacttcttac actccctagt tttttgaaaa tcaaatgtat
    29821 cattcggctc gatacaactt aacagtggta agtcttcttt aataatttgt gcaacaacat
    29881 tcacattgtc atgtgcacta agcgtttgtc tcacaatttg catttcgcct tgataacgtc
    29941 cgttattcaa attatcaacg gttactgaac caatgcgtcg ttgcgtcgta aactgtggtt
    30001 gaatcgaatg tggacatatt tgtcttgacg tttccgaacg aatgacattt tccggattat
    30061 ccgggcgtac tttatgacac atatcgaaaa ggtaagtcac tgtcgtatca aacacttctt
    30121 caatacataa cgtgaaatgc ctatgcttgc aaaaatctat aagttgtttt gcctgcctca
    30181 tttcaatcaa tgagtctcca actaacactt cagatacacc agtttcttgt aataatttag
    30241 ctgcaacgac aggatgacta tgtctcgttg cttcaattgt tggcaagcct ttatgcaaag
    30301 gacctcgcaa atcactccct acaataaaac catatatttg tgcctttgga ttaaattgat
    30361 aaatgagttc atttttctta ttgaccaagt caacagataa tcccgtatct ggtcttggat
    30421 aatagttatg acaaaatgaa agtaatgtaa aatcattcaa ttgttgatgt aagcttgtta
    30481 acaattcccg ggaaataata cttgcattca aacagcactt taaaccctgt gccattatcg
    30541 cttcgattgc ctcaattgat gtactatgat cgatacgaat cataaattgt gcatcatatt
    30601 gtcgaagatg gtcataaaaa gatggtgtta aaatagatgg attagcatct atgaggtaag
    30661 tcacttgttc atgttttaat aaattgagta gttttgtgaa ataatgatat tttgtctcgt
    30721 catcttcttc tggtatttgt acagatgtaa aaatcatttg gtaaccttgt ttaatcattc
    30781 gcttaatata cgcttcatct aaaggttgtc ctaaatacac tgaaaagcct gtcaaagtag
    30841 ccctccttaa caatataatt attaggaaaa tatagttgag ttgtgtaatc gcttacattt
    30901 ta
//
LOCUS       DAKTLH010000016        30098 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.016, whole genome shotgun sequence.
ACCESSION   DAKTLH010000016 DAKTLH010000000
VERSION     DAKTLH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 30098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30098
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.016"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(14..1360)
                     /locus_tag="O0394_000666"
     CDS             complement(14..1360)
                     /locus_tag="O0394_000666"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCY0498766.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
     gene            complement(1577..2167)
                     /locus_tag="O0394_000667"
     CDS             complement(1577..2167)
                     /locus_tag="O0394_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCY0498767.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
     gene            complement(2321..3583)
                     /gene="clpX"
                     /locus_tag="O0394_000668"
     CDS             complement(2321..3583)
                     /gene="clpX"
                     /locus_tag="O0394_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCY0498768.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            complement(3734..5035)
                     /locus_tag="O0394_000669"
     CDS             complement(3734..5035)
                     /locus_tag="O0394_000669"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCY0498769.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            complement(5083..5193)
                     /locus_tag="O0394_000670"
     CDS             complement(5083..5193)
                     /locus_tag="O0394_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498770.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            complement(5198..6127)
                     /locus_tag="O0394_000671"
     CDS             complement(5198..6127)
                     /locus_tag="O0394_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498771.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLADFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLK
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            complement(6146..6754)
                     /locus_tag="O0394_000672"
     CDS             complement(6146..6754)
                     /locus_tag="O0394_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCY0498772.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            complement(6896..7252)
                     /gene="rplT"
                     /locus_tag="O0394_000673"
     CDS             complement(6896..7252)
                     /gene="rplT"
                     /locus_tag="O0394_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCY0498773.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            complement(7299..7499)
                     /gene="rpmI"
                     /locus_tag="O0394_000674"
     CDS             complement(7299..7499)
                     /gene="rpmI"
                     /locus_tag="O0394_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCY0498774.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            complement(7528..8055)
                     /gene="infC"
                     /locus_tag="O0394_000675"
     CDS             complement(7528..8055)
                     /gene="infC"
                     /locus_tag="O0394_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCY0498775.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     misc_feature    complement(8084..8209)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(8284..9777)
                     /locus_tag="O0394_000676"
     CDS             complement(8284..9777)
                     /locus_tag="O0394_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0498776.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     gene            complement(10203..12140)
                     /gene="thrS"
                     /locus_tag="O0394_000677"
     CDS             complement(10203..12140)
                     /gene="thrS"
                     /locus_tag="O0394_000677"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCY0498777.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            complement(12338..12460)
                     /locus_tag="O0394_000678"
     CDS             complement(12338..12460)
                     /locus_tag="O0394_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498778.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLKQRYLIHKLY"
     gene            complement(12553..13473)
                     /gene="dnaI"
                     /locus_tag="O0394_000679"
     CDS             complement(12553..13473)
                     /gene="dnaI"
                     /locus_tag="O0394_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCY0498779.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            complement(13473..14873)
                     /locus_tag="O0394_000680"
     CDS             complement(13473..14873)
                     /locus_tag="O0394_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HCY0498780.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSAGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            complement(14874..15344)
                     /gene="nrdR"
                     /locus_tag="O0394_000681"
     CDS             complement(14874..15344)
                     /gene="nrdR"
                     /locus_tag="O0394_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCY0498781.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            complement(15554..16579)
                     /gene="gap"
                     /locus_tag="O0394_000682"
     CDS             complement(15554..16579)
                     /gene="gap"
                     /locus_tag="O0394_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCY0498782.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            complement(16749..17372)
                     /locus_tag="O0394_000683"
     CDS             complement(16749..17372)
                     /locus_tag="O0394_000683"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCY0498783.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
     gene            complement(17388..18260)
                     /gene="mutM"
                     /locus_tag="O0394_000684"
     CDS             complement(17388..18260)
                     /gene="mutM"
                     /locus_tag="O0394_000684"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCY0498784.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKYGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            complement(18276..20906)
                     /gene="polA"
                     /locus_tag="O0394_000685"
     CDS             complement(18276..20906)
                     /gene="polA"
                     /locus_tag="O0394_000685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCY0498785.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            complement(21200..22687)
                     /locus_tag="O0394_000686"
     CDS             complement(21200..22687)
                     /locus_tag="O0394_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498786.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLCFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQIIILSMIAALIIYLLLR
                     SDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFVNVIVCGYFAFS
                     IFVGIYLSINDRKTQITLYSLLIAICVSASTLVIVTPIEPGNFLIVYTIHVIILIILL
                     KEFRKYKSINVNFIKGASLVLAVIYLSAFIYVHYEHEKRVQLLKQQIKDHPRQEVYTV
                     ESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
     gene            complement(23186..24847)
                     /locus_tag="O0394_000687"
     CDS             complement(23186..24847)
                     /locus_tag="O0394_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HCY0498787.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKKIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
     gene            complement(24847..25551)
                     /locus_tag="O0394_000688"
     CDS             complement(24847..25551)
                     /locus_tag="O0394_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0498788.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVES
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            complement(25941..27209)
                     /gene="icd"
                     /locus_tag="O0394_000689"
     CDS             complement(25941..27209)
                     /gene="icd"
                     /locus_tag="O0394_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCY0498789.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVENEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            complement(27258..28379)
                     /locus_tag="O0394_000690"
     CDS             complement(27258..28379)
                     /locus_tag="O0394_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HCY0498790.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            28727..30088
                     /locus_tag="O0394_000691"
     CDS             28727..30088
                     /locus_tag="O0394_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0498791.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAK
                     SNK"
BASE COUNT         9287 a         5679 c         4109 g        11023 t
ORIGIN      
        1 catattaata tgcttattca aaactaaaga tacgtcgcgc tgaaatttcc ttgaccttgc
       61 tttctttttg ttgcttcgct tgctcatgag gacattcagc ttcgatatca aatatatttt
      121 gaaatagctc taatttttca ttacttttct tatcactact taattctttg gcctgtttaa
      181 taggatcttt caacatttga ttgatgatac tttttgtatg tttagatata attttacgtt
      241 ctctttcgct taaccctggc aatttacgat caatactgtc catcgtttct gcttgaattg
      301 ccatagcttt ttcacgtaaa gctctaatca ctggaacaac acccaacata ctaatccact
      361 cattgtgtgc atgtatttct gcaggaattt gttccgaaat tgttgcagcc gctaattgtc
      421 gctcacgtaa gtttgcatca actaaacctt ttaagtcatc aacatcataa ttaaagatgt
      481 ttgtgatggc actaatacca ggttcaatat ctcgaggaac tgcaatatca atcaatacta
      541 gtgaatcttg ctttctattt tctgcaattc tttctatcat ttcatttgta atgatataag
      601 attgtgcact cgttgaacta atcacaatat ctgcactttc aagtaaattt ggtaatgatg
      661 atagttcatc atatttcact tgatgctttg ctgctaattt catagcattt tcaattgttc
      721 tatttactac tgtaatatca gtaattccag aaccaagaag atttaatagt gataattcac
      781 tcatttcccc tgcaccaata atgatagctt gcttactttt caatttgcca aatacttttt
      841 tcgccaactc gaccgcagca taagacacac ttacagcatt atcagctata tctgtttcat
      901 tatgtgctct ttttgcaaaa gtaattgcct gtttaaatag atgattaaaa attgttcctg
      961 tcgtacctgt gctttgcgct aagaaaaatg catctcttat ttgacctaaa atttgagttt
     1021 ctccaagtac gattgaatct aaaccagaag tgacacgcaa taaatgttct actgcttcgt
     1081 cccccacttt tacttctgac attgctttaa tatcatctac ttcaaatcca aatgcacgag
     1141 ctagaaatcg ttgaatatag taacgacctg tgtgaatttg atcaacaaca gcatatactt
     1201 cagttcgatt acatgttgat aatatgacat tttctaaaat agatttagtt tcatataaat
     1261 cttcatgggc aattcgtaag gcatcatctc taaaagcaac ttgctctctt agtgcaacat
     1321 cagctgtgcg atgatttata ctaattgcaa taaaatgcat ttgaaacgcc cccatatata
     1381 ctaacaatac aaatattata acataattta aaatccaatt aagtacgatg cttaaataaa
     1441 ttataatctt tctaatttac ttttgagttt ttgacaaaat gatgaacgtt gaaaaatgtt
     1501 atcaattgtg tactaattaa aagtttcgtg cttacattat ttttaaaatt tttgtataag
     1561 ttttgacgta caaaaactat gaaatatacg gttcaattaa attccatatt tgttgttgtt
     1621 tattattttg aattgatgaa taacttacaa ttgtatcgtc tgggtccata tctaattgtg
     1681 tcttaatatt tttaatatgc ttttgaacct taccttttgg aattttgtct tctttagtgc
     1741 atataactaa agtaggaata tcaaaatgtt tcaaataatt gtacattaag atatcatctt
     1801 gtgttggatc atgtcttaaa tcaactaatt gaataactaa ttgcaaattc tctctcttag
     1861 ttatatattc ctcaatcatt ttcccaaatt tttcacgttg tgttttactt actttagcat
     1921 atccataccc tggaacatcc acaaaaataa gttgttcatc tatattataa aaatttaacg
     1981 tttgcgtttt gccgggttgc tgtgatgtac gtgccatatt ttttctgcca atcatactat
     2041 tgataaatgt agacttacct acattagatc gaccgctcag tgcaacttca gacaattctg
     2101 tttctggata ttgttcttct tttactgcac taatgattaa ttctatatta ttaggattaa
     2161 ctttcatttt atatcctcac ttttttatat tctctttcaa ttctaccatt atataatatt
     2221 tgaacctttc ggtatacatt ataaaaaagc tccgatcaaa gttaaactat ttgagcgtca
     2281 actttgattg gagcttttca cttttataac acatcaatga ttaagctgat gttttactat
     2341 tattaattaa attgccttct gcgtcgtata gttctggttc agtttcttca ttaattgttt
     2401 gtgctgtaat aactaccttc gttacatttt cgttagaagg cacatcaaac ataatatcga
     2461 ttaacgattc ttctatgatt gaacgtaaac cacgcgcacc tgtttttctt tcaattgctt
     2521 tttcactaat tgctgataaa gcttcttcag tgaactctaa atccacatca tctaattcca
     2581 gcattttagt atattgtttc acaagtgcat ttttaggttg cgttaagatg tttttcaacg
     2641 cagttacatc taatgtttct aaattagcta caattggcac acgtccgata aattcaggaa
     2701 tcaaaccata ggcttgcaaa tcttctgggc gaatttgtgc taataatgct tgttcgtcat
     2761 atttatcagc ttcattgctt gagaaaccaa taactttttc accaagacgg cgcttaatca
     2821 cttcttcaat accatcaaag gcaccaccaa gaataaataa gatatttgtt gtatcaattt
     2881 gaatcatttc ttggtttgga tgtttgcgtc caccttgtgg cggaacactt gcagtcgtac
     2941 cttctaagat tttaagcaat gcttgttgaa caccttcacc tgaaacgtca cgtgttatag
     3001 atgtgttttc agatttacgt gcaattttat caatttcatc tacataaata ataccttttt
     3061 cggctttatc aatgtcaaag tcagctgctt gaattaatct caacaagata ttttcaacat
     3121 catcgcctac ataaccagct tcagttaaac ttgtcgcatc tgcaattgca aatggtacat
     3181 tcaacgtctt ggctaaggtt tgagctaata atgttttacc actacctgtt ggcccaatta
     3241 atgcaatgtt acttttttgt aattcaacat catcttcttt tggtcctaat tgttgaatac
     3301 gcttatagtg gttataaaca gctacagcta aagatttttt agctttttct tgaccaataa
     3361 catattcgtt taaatgatcc ataatttctt taggagtagg taattctgtc atcgcttcag
     3421 aagtgttttg agctaattct tcttcgacga tttctgagca taattcaata cactcattac
     3481 aaatatatac accacttcct gctacaagtt tttttacttg atcttggtct tttccgcaga
     3541 aagagcattt caaattttct tcatcttcat tgaatttaaa cattcttttt acacccctat
     3601 tcgttaaaga ctatactaga ttggatgtta caatgcaaca tattaacata caaacttttt
     3661 gcttaaagaa tagtagcaga tacataagct atgcatctgc tactcatttt tgtaatttaa
     3721 tatttaatga agattaatct tctttagttc cttcaacgaa ctttgcgtta tctcttaata
     3781 aatcgataac tttttggata cgaacatcat ttttaatgat atcagtatta cctaaagtat
     3841 ttttgatatc ttcaactgag atattaaatt gtttactcat tttttctaat tctttatcga
     3901 tatcttcatc agtagcttcg attttttcag cttcagcgat cgcagttaaa gttaagttag
     3961 ttttaacacg ttgttctgca tcgtctttca tttgctctct taattgagtt tcatcttgac
     4021 ctgagatttg gaagtacgtt tgtaaatcta aaccttgttg ttgaattctt tgtgcaaatt
     4081 cagacaccat acgatctaat tcagtattaa ccattgcttc aggaatatcg attgttgtat
     4141 tatcagtagc ttttgtaatc gcttcttctt tttcaacatt ttcagcatct gtagcttttt
     4201 gttcagctaa acgtttacgt aagttttctt tgtactcgtc tactgtattt gcttctgcat
     4261 ctaattcatt agcaatttca tctgttaatt ctgggacttc tttaaattta atttcgttaa
     4321 cttttgtttt gaaagttgct tctttaccgg ctaattcttc agcatggtat tcttctggga
     4381 atgttacgac aacatctttt tcttcgtcaa ctttcatacc ttctaattgc tcttcgaaac
     4441 caggtatgaa tgaacctgaa ccgatttcta aatcgtaacc ttcagcttgt ccaccttcga
     4501 attcttctcc gtcaactgaa ccactaaagt cgatgttaac tgtgtcgcca ttttcaacaa
     4561 caccatcttc tttaacgacc atttcagcta aatgtcctaa gctgtggtca atcgcttctt
     4621 gtaactcatc atcagataat tcagtttctt gtttttcaat ttcaagacct ttatagtctc
     4681 ctaatttaac ttctggctca actgtaactg ttgcttcaaa aatgaaatct ttaccttttt
     4741 caatttgagt aacacttact tctggttgtg caactggttt aatatcagtt tcgtcaattg
     4801 cttcaccata agcatctggt aataaaatgt cgatagcatc ttgatataat gcttctacac
     4861 caaagcgttg ttcaaaaatt ggacgtggca ctttaccttt acggaatcca ggtacgttaa
     4921 tttgtttaac cacttttttg aatgcttgat ctaacgcttt gtttactttt tctgcaggaa
     4981 cagtaacagt taataaacct tcgttacctt cctttttttc ccaagttgct gtcatgtata
     5041 tatacctcca tgattaacta atttattttt tcaacttccc tattatatca taggtctatt
     5101 ccctatacaa acattgaaat cacaacgttt atatatttgt aaatcaactt ttttcgtcaa
     5161 aactatatta aaatgctgag tattattttg caacaattta actattatta ttcaaatcta
     5221 attgctgaat aaaattaaat gcatcgatac tttgtaattc acatttaagt cctaacatat
     5281 ttttgaaata acattcgtat gcattaatcc atttattcgt ttcaaacaaa gtttcaatat
     5341 caattggata aatcatgata gagtgattat tcataatatg gtgtgcttct tcagcgatat
     5401 gtaaagcacc atcatttaat gtttccataa cgttaggtat aactttttct ttaagtgttg
     5461 tatgttctag cccttttaaa ttagctggta caaaagttac atccatacca tacttttcaa
     5521 ttgtcagttc ttgtgtacaa tttgcgaacc ttaaatactc aatcattaaa ctaatgaggt
     5581 tataactgta cgtattagat tttaatatat ataataccgt ttcttgaaat tgaaaatgac
     5641 cattgtctat taacttcaaa atcaagtgcg tctgttccct cattgataac gtatcaaaat
     5701 cagctaatga ctgagtcaat cgtttttcat cttcaattaa ttttgactta gcaaattctt
     5761 taagaggatg taatgccatt cttgttttgt gatctttgac ttcatcaata atttgatgaa
     5821 ttacttctac cgcttcaaaa tattgtccca aaccaatcaa acttttcacg taataaatca
     5881 tcaatgcatc atattgttgt atgccagttt tcaataagac aattgtttct tctcttaatt
     5941 caagaaatga ccccgtctca tataacatac ggcatttcat cattgctatt tcttcagtga
     6001 gttcaaattg acgttcatat tgcacaatat attcatacat caggtcatag ttttggtctt
     6061 gttcagcacg ttttatatct ttatataact ttttcgaaga gttaggaaac tgaataatat
     6121 ctgacatata tgtcacctcg tttgattata aatcttgaag gattaatgca ctccacgttt
     6181 cgaaatcatc atatgactct atgttgcctt tttcaaccca ttttattcgt aaagtatctg
     6241 tttctttagc ttctacatca ttcgtactta cagtgatttt aaatacaaca ccaatatgta
     6301 ccttgcccac ttcattatta tcgtcattaa taaaaccgat atattccata ttttgtgaat
     6361 cttgctcact taaacctact tcttcttcta attctctctg tgcattaact ctcaatactt
     6421 cgttaataga ttctgctcct ggaacatcat tcatatgacc gcctacacct attgaagatt
     6481 gtccatgcaa tcgagcttct ccaccgccag ataatcgttc atacactaat atctcgccat
     6541 gctcattttc aagtaaacaa taagaaatga gttgtttata tgatggatcc tcttccatat
     6601 cgccgcgtcg cttaacttca tattgactta gcgcatcaaa aatattttga ccttctggtt
     6661 tattcttatt taaaaaccca ttgaaagtat tcttttcatt attaaaaata atttctctag
     6721 gtactacaat gatttgttca tcaaatttag acatcgtata aaactcctca ctttaatgtt
     6781 tgttctctta aatgcatttt agcaaatatg tcatatttta gcgattaaat gtggcatttt
     6841 gctatttaaa aaataaaaaa gagatgacaa tgccatctct tttttataag tgttattatt
     6901 ttaaagcatc tttagcttta gttactaatt gagcaaatgc tttttcgtca gaaattgcga
     6961 tttctgataa cattttacgg ttaatgtcga taccagcttt tttcaaaccg ttcattaaac
     7021 gtgagtagct catttcatgt tgacgagctg ctgcgttgat acgtgtaatc cataatttac
     7081 ggaagtcacg tttacgttga cgacggtcac ggaaagcata ttgacctgat ttcattactt
     7141 gttgcttagc tactttgtat aatgtatgtt ttgaaccgaa gtaaccttta gctaatttaa
     7201 tcgttttttt acgacgcgct cttgttactg ttccaccttt aactcgtggc ataaaaaatt
     7261 cctccttagg taattcctac cgatttctgt atttgttctt attttttgta tgctaataat
     7321 tgttttacac gtttcatatc gctcttagac actaatctag ctttacgtaa ttgacgtttt
     7381 tgtttagtgc tcttgtttgc gaataagtga gatgtgaaag ctcttgaacg ttttaattga
     7441 cctgaagcag ttcttttaac acgtttagct gctccgcggt gagttttcat ttttggcata
     7501 atataagtcc tcctgtaata gctttaatta tttttcagct gttggcgcta acatgataaa
     7561 catttgacgc ccgtccattt taggtttttg ttcaactgtt gctatatctt tgcattcatc
     7621 tgcatatttt tctagcacac gttgaccaat ttccttatgc gtaatggcac gccctctgaa
     7681 acgaatagat actttacatt tatcgccttt agttaagaat ttacgtccgt ttttcaactt
     7741 cgtttggaaa tcatgttcct caattgttgg acttaaacga atttctttaa cattgataat
     7801 tttttgtttc tttttcattt ctttttcttt tttctgttgt tcgaatttga atttaccgta
     7861 atccataatt cttgcaactg gtggtttcgc attcggtgca acgaccacta agtctaaatc
     7921 tacacgttca gccatttcta aagcttcacg ctttgattta acaccaattt gttcaccatc
     7981 ttgaccgatt aaacgtaatt cttttgcacg aattttgtca ttgatttgag tttgatcttt
     8041 tgctatggtt gacacctcca aaatttttac gaaatttgca ccaagcaaaa aggaagagca
     8101 ggtataaaat acccgctctt ccttatacac agttatgtgt aatgtgatta acctgccaac
     8161 tgctttatgc gtcgctacag gtgagaagcg ggtgcttcta cttggttcgt ttcgtattca
     8221 acgttattaa tcatatcaac aattcacatt taagtcaaca ctataactgt aattattttt
     8281 attttaacct tttatttcat ccattgacac gtcttgacgt aaatctactt gttctaatgg
     8341 aatttttttc gttttatatc gaagcttatg ataaataaag aatgctaaaa atactggaat
     8401 tcccatatac gtaattaaga agcgactaaa attaaaatct cctgttttaa taaagtcaac
     8461 atcttgacca ataattacta caatacataa aaagccagca aataatggtc cgaatgggaa
     8521 taatttagca gtatatttta atttagattt gtcataattt tgtttatcaa atgctcttct
     8581 aaatcgataa tgacttactg ctatacctac ccaagcaata aaaccagtta aaccacttgc
     8641 tgcaacgata tattcgtatg caccttttgt aaggctttgt aatacgaaaa ttatcactac
     8701 aatgattgct gtaacgagta acgacatata tggcacaccg tttttatttg ttttaccaaa
     8761 tgcttcaaac gctaatttat ctttactcat tgaatatagc attcgagttg aagcatacat
     8821 acctgagtta cctgctgata acacagacgt taaaatgact gcattcataa atgatgctgc
     8881 aaacgcaaat ccagcatttt taaacactaa tgtgaatgga gacgttgcta cattatcact
     8941 accccccatt aatgcactac tatcataagg aattaacata ccgataacaa aaatggctaa
     9001 aatgtaaaat aataaaattc tccagaatac ttgtttaatt gctttcggca cagcacgttc
     9061 aggattttct gattcaccag ccgtaatacc aattaactca gtaccttgga atgagaaacc
     9121 agcgattaag aatacaccta gaattgataa caaacttcct cctaagttgc caccaagaat
     9181 tggaccttca cctttattaa atatttcgaa tcctacaaca tgaccaccca tgattccgac
     9241 aatcgttaat aaaccaattg caatgaaaac aataactgta accactttta tcaatgccaa
     9301 ccagtattca ctttcaccat agacgcgaac tgataacgaa ttcagactaa aaattataac
     9361 taagaacaac gcactccatg cccaagcggg tatgccttgc aatggtgtcc aatattgaat
     9421 gacttgtgct gcaatcgtaa tatctgctgc tacagtcact acccagttaa accaatagtt
     9481 ccaaccaagc gcaaacccta aagatggatc aacaaatctt gtagcatatg tactaaatga
     9541 acctgatact ggcaaatacg tagccatttc gccaagtgac gtcattaaga aaaataccat
     9601 tattccgata attgcgtatc ctattaatgc acccaaagca cctgcatcat gaattgctcc
     9661 accagaagtt acaaataaac ctgtaccaat acaaccccca atcgcaatca tagaaatatg
     9721 acgatcttta agtccccttt tgacaacatt gttactttca ttttgaactt ttgacatata
     9781 agctcccttt ccatgtttta gaggccaaga aataaataac ctttattcac tttcataccc
     9841 ttttttacag aacatagtga tattaaaagt aaaaaagtgg ctacatacct ttaagatatg
     9901 caaccacttt ggcaattatc tatataaggt agcacatcac attctgtgac agtacagttc
     9961 ctattcgaaa actgtcccaa tagatataat ttatggttta tatctatttc ggcatcttta
    10021 cctttcactt gttcaactta tgtaccataa atacttctga caagttacta attaaacatg
    10081 caacctctaa ctcaatttaa tattttaact aacttgtaat atacaggatt catcacgcat
    10141 aatcaaccct gtaaaacttg atacgcaata aaagttttaa agcattttat tgcgacaact
    10201 gtctatctat gttttttcaa acgaatttca tcaactagat tccagataaa ttcatctttt
    10261 tcaactgttt cttggtcttg cgatccatat tgacgcacat tcacttgatt attttcaact
    10321 tccttatccc caactacgat ttgataaggt attttttgca tttgagcttc tctgatttta
    10381 taacccattt tttcattacg gtcatcaata cttacacgaa cgccttgaga tttcaattca
    10441 tcttgtaatt ggcgcgcata atcataatgt aaatcaacgt taactggaat gatttgaact
    10501 tgttttggcg ctaaccaagt tgggaatgca cctttagttt cttcagttaa aaatgctaca
    10561 aatcgttcca ttgttgatac aacaccacga tgaataacaa ctggacgatg atgttcacca
    10621 tcttgaccaa tataagttaa atcaaaacgt tctggtaata agaaatcaag ttgtgctgtt
    10681 gataatgtct cttctttacc catcgctgtt ttaacttgaa catctagttt cggaccatag
    10741 aatgccgctt caccaatcgc ttcttcgtac gataagccaa gctcatccgc tgcctcttta
    10801 agcatatttt cagctttatt ccacatatca tcatcatcaa agtacttttc tttatcttca
    10861 gggtctctat aacttaatct aaagctataa tcctcgaaac caaagtcttt atacacatca
    10921 ataatcatgt ttacaacgcg tttgaattct tctttaattt gatcaggtcg aacaaagata
    10981 tgtgaatcat ttaaagtcat accacgaaca cgttgtaatc ctgatacagc accacttgct
    11041 tcatatctat gcatcgttcc tagctcagcg atacggatag gtaattcacg atatgaatgt
    11101 ggtttattcg cataaatcat catatgatgt ggacagttca ttggacgtaa taccatagat
    11161 tcagtttcat ctaactgcat tggtgggaac atatcttctt gatagtgatc ccagtgacca
    11221 gatgttttgt ataaatcaac attagcaagt actggtgtat aaacgtggtc atatcccatg
    11281 ctaacttctt tatcaacaat gtaacgttca atttcacgtc taattgttgc accgttaggt
    11341 aaccataatg gcaaaccagc accaactaat tggctatttg tgaatagttc taactcttta
    11401 ccaattttac gatgatcacg ttctttacgc tcttctaaca tttgtaaatg tgctttcaat
    11461 tcttttttat caaagaaagc agtaccgtat atacgttgta acattttgtt gttactatct
    11521 ccacgccagt atgcacctgc tgtagataat agtttaaact ctttaatttt agctgttgat
    11581 ggaacgtgaa ctccacgaca taaatcagta aaatcacctt gactatataa tgttacattt
    11641 tcatcttcag gaatcgcgtc gattaattct aatttgtatt catcattgct gaataactct
    11701 ttcgcttcat ctcgtgaaac cacttttcgt tcgattttca tattttcgtt aacgatttgt
    11761 ttcattgttt tttcaatttg ttcaaagtca tcagatgaga tgttttggtc aatgtcgaag
    11821 tcatagtaga atccaccttc tattacagga cctacaccaa atttaacatt accatataac
    11881 cttttaatcg cgtgtgccat taaatgtgca gtagaatgac gtaatacctc taacgcttct
    11941 tcactacctg gtgtcacaat ttcaattgat ccatcagttt caagcggttt agttaaatct
    12001 acaagttgcc cgttaaattt gccggcaaca gcttttttac gtaatccagg actaattgat
    12061 tgtgctatat cttcagtagt agtaccttta tcaaacgcct ttttattacc atctggaaat
    12121 tgaatattaa tttgttccat gttaaaccct cctatttgct ttataaatac ttttatctga
    12181 cctcgttaaa aaattgaaca acaatcatcg ttttaacatt aaacctatta acaataaaca
    12241 gggaatttat gacaccaaca aacatttatg tctgaaaaaa acaaaaaatc ccgcccctat
    12301 acaagggacg agatcgtcgt ggtaccaccc tagttatcta atacaattta tgtattaaat
    12361 atctctgctt aagataacgg tcttgatccg ggtattcatt acaaatacca taaatgaagt
    12421 agtaatcatc taatttatta accatattct catcaaacaa tggctttctg tgtaatattg
    12481 attagatcat cttgtcttca atcatttaaa cgatttagat ttgtattcat tatacaccaa
    12541 tcattttaaa attcaattgt ttctgaaatt ttctcctgat aaaaagtatg gtgttgacaa
    12601 agatttgaca cgttcaataa tacgtgctgc tttagtcttc tcttcaccat cacgagtcat
    12661 cgctaaatga tgttccaatt cactatagtc aaaattagaa ctaaagaatg ttggtaattc
    12721 atgaaccatt cgataatgta gcaaaggtcc aattacctca tctctcaccc atggagtcac
    12781 ttcttcagcc ccaatatcat caagcattaa aatgtttgct tctcttacgc gatgtaattt
    12841 cttttcaaaa gaaccatctt taaagccacc ttttaatgtt ctaataaatt ccggtaaata
    12901 aataattgtc gaacgtacct tcttagattt gagctgattc gcaattgcac ctagaataaa
    12961 agatttacct gtcccaaatg gaccataaag gtaaaggcct ttcacttgtt ccccattagt
    13021 tattgctgta caaatatcat ctgctgccat agctacatca agacggtctc gatgattcat
    13081 ataaatatct ttcaatttgg catttaaagt atctcgttgc atatgatgag atgtaattag
    13141 ctcagcttca aagcgttctt cgtcgtactt gattttacat gggcattgta aatagcgtat
    13201 tttaattcgg ttattatcaa catataactc aggcacatgc ccctttacga aatttggaca
    13261 atcagcaaat ttatgaccgt cataatgttt ttgttgatct ttatactctt gtaacacatt
    13321 taagtcttca tcaatcatag cattcgttaa ttcagctcga tgcgcttcca aaaattgctt
    13381 aacatctggg tcattgatta cttctttttt tatcttttct attctttttt caaagtcctg
    13441 cgacgtgtta attatacttt taaattgctt cattattgac tgtcctcctc ccatttttta
    13501 gataatttat ctagaaatgc ttgtcgatct tgctctaatt gttgatcatc tacgctatta
    13561 tctttagccg aatcttcttc actaggttta tctctatttt ctaaccattt aggtgttttt
    13621 tcttttgaaa tacgattacg ctgcccatag tatgaaccac gcttttggta atttccgcta
    13681 gaaccctcat ttttaggttg attaactttt ttagcgtaat tatatgcttc tttagctgtc
    13741 ttaatacctt ttttcttcca atttgatgct atttccaaaa tatacgcttt aggaagtttc
    13801 atatcttctt ttaacatgac aaattgcaac aaaatattaa tgacgccaaa agacattttt
    13861 tcacgttcaa ttaattcttc aaccattgtc ttttgcgata tagttggttc tgattcagac
    13921 caagaagcta acatatcaat tggactcgtt tgttcaagta actcaaacca ttcatcactt
    13981 tgtggctttg gattcacttc tgaagatttg cccgccgaag atgatgtagc aggagatttc
    14041 acctgtaatt taggcatttg attttcgtgt tccattaagt aatacgagcg tgcttgttta
    14101 cgcatttctt caaaggataa ctgttgtcca cttgtaattg aatttaaaat aacatgcttc
    14161 atgccatctg ctgttaaacc atataaagtc gcgagttgtg taattaaacg ctttgcatct
    14221 ttggtaacaa tgtcttgact aataaaatgt ttacctaaca tttgtctcaa catttcaaag
    14281 tcaaaagatt catttgataa atcgatacct tggtacggtt cattaatcgg aatatcactt
    14341 gtatcgatat ctatttttgt agacggcact ttaaaaacat cagtaaattg tcttgttacc
    14401 tgtttaaatt cactcaaatc aatttgttga tactcaaagt atttcttcaa ctcatgaaat
    14461 cgacgatgct cgacttcact atataaaaag attgacaaca ttggatcatt aaaaaataaa
    14521 tgtgctgaag gcggttgaat taattggtaa acaaattgtg tttcttgttc atcatgtttg
    14581 acaaacgcct ttaacaatcc aatcgcttca agtaagtcca tttgttgtct aaactctagt
    14641 aaattaattt taagttcatt cataaaaata taatgagaaa gaatcaatgt ttcattatga
    14701 ctttctttaa cgaattgagt cataaaatga tataaaccca ctgcttgcgt tccaattagc
    14761 ggtgtataca gtcgattcaa tacctctaaa tgattcgtat ttaaatcaaa gtgttgcata
    14821 actttgaatt gatcctttgg tcttaagccg aattcgaagg cttgtcgtcc catttaagca
    14881 tcactccgtt tgttttcgct taaaatccct tgcatcgatg ctaacaattg atcaacatct
    14941 ttaaattctt tatagactga tgcaaatcta acatatgaaa cttgatcaac atgcattaac
    15001 aagttcataa cgtgttcacc tatatctcgt gaagacactt ccgtatgacc ttcatctcgt
    15061 aattgccatt caaccttgtt agttatgtct tcaagttgtt gatatctaac tggtcgtttc
    15121 tcacaagaac gcacaagtcc attaagtatc ttttctcttg aaaactgctc tcttgtgcca
    15181 tcttttttca caactataag ctgactaact tcgatatgtt caaatgtagt gaaacgtgtt
    15241 ccacaatttt cacattctct tcgtcttcga atggcattta attcatcggc atgccttgaa
    15301 tctacaactt tagattgtgt agaattacat ttcgggcatt tcattacatc accctcttta
    15361 ttttgattat gcctaattat actataaatc tagagatgaa aaaagaatcc ctcaatttaa
    15421 ttcatttaac caaataatga aacaataaaa aacattatat cgttacttat taagtaattt
    15481 gcatgacaat attattgtat taaaaataaa aaacctaact ccgaagtcag agttaggcta
    15541 taaattaatt gtattaactt gcacttacag tttcttttga tgtcaaaagt gctccaattt
    15601 gctcagcaac atctacaact ctatttgaat aaccccattc attatcatac caagcaataa
    15661 cttttacttt attccctgac atgaccattg ttgattttgc atcaataata gctgaatttg
    15721 gattagtatt aaaatcaaca gacactagtg gttgatgttc gacttctatg ataccttcta
    15781 aacctgcatt ttcaaaagct tggtttactt cttctgcagt tacttctttt tctaaatcaa
    15841 caactaaatc aacgagcgat acattctttg ttggtacacg taatgccatg ccgtgtaatt
    15901 taccttctaa ttctggtaat acttctttta aagctttcgc cgcaccagta gaagtaggaa
    15961 taatgctttc attacatgaa cgtgcacgtc ttaaatcttt atgtggatta tcaatatttt
    16021 tttggtcatt tgtaatagcg tgaacagtag tcattaaacc attaactatt ccaaactgat
    16081 tatttaaaac ttttgcaact ggaccaatgc aattagtagt acatgaagca ttactaaaaa
    16141 tgtcaaatgc ttctatatct aattggttat catttacgcc tttaactacc atttgaacat
    16201 gtccaccttt tgaaggacca gttaacaaaa cttttttggc acctgcttta atatgtgcga
    16261 tggctttatc accatgatta aatttaccag ttgcatctat agcaatatcg atatctaatt
    16321 ctttccatgg caagttttca ggattgcgat cagcaaccaa tttaatttta tgatctccaa
    16381 cttgcaatcc attttcaatc ggttcaactt ttagattata ttttccatgt gtcgtatcgt
    16441 aattgattaa atgtgcaatt gtttcgggtg gataactagc atttatcgct actacattta
    16501 aatttttatt ttgtaatgca atacgtaata ccattcttcc aattctaccc ataccattaa
    16561 ttgcaatatt cgttgacatt aaaagcaccc cttgatcttt atgtgttata ctttatggaa
    16621 ttagtatagc atattatatg aaatttgaaa gcgttttccg ttgatttatt tatctttttt
    16681 ataaaaaata gtcattactt acatacatca acgcgtacgt aaagtaatga cttatttata
    16741 gtgtaatatt aatcttcttc tccgtaattc ggcttttcaa tataaccttc ttcttctaac
    16801 aatctctcaa ggttttgttt taattcaagt ttatccccta aattatcgat aacatgatcg
    16861 gccattcggc tttttttatc aatagaaatt tggctataga cacgtgcttt cgcatcttct
    16921 aatgacaaat tattacgttg cattaaacga tccatttgta tactttcaga agtgtataca
    16981 acccacactt cgtctactgt attttccaat tcattttcaa ataataatgg aatatccatg
    17041 attacattat atccttgttt taaatattct tgcttttctt cttccataat atctcgcacg
    17101 ataggatgta tgatagcatt taattctaag cgtttttctg gatgattaaa cactagatca
    17161 cccatataac gacgattcat ctcaccattt tcatcaattg cttcatcacc aaagacttct
    17221 cgtacttgag ctaaaccttt actccccttt ttaacagctt ccctggctgc tttatcagca
    17281 tctactactt taaaaccgaa tacggataag agttctgata ctgttgattt tcctgaggcg
    17341 attccacctg ttagaccaat aactttcggc ataatttcac tctttcttta tttttgacat
    17401 actggacaat aatgactatt tcttgtcgcg atgatttttg tttcaatttg acttccacac
    17461 actttgcata ccggctgctt atatacatta agatgcaatt gcatctcacc agtttttcca
    17521 tcagcatgac gataatctga aatacttgta ccgccatatt taataccttc ttctagtact
    17581 tctctaacat aataaaaaac catttcttgt tgttggtgtg ttaagtcttt tactttttta
    17641 tctggtaaaa cacctgcacg aaacaacgct tcacatgcgt aaatatttcc acaacctgcg
    17701 attactttat gatccaaaat cacttgtttg attggtttat tcttattaga ctgttgatga
    17761 attcgattta aataatacgt caatgcttca tttgaaaaag gttcaggcgc tatttctaaa
    17821 aatgaaggat aagatgctac agacgcaaca tttctaattt ctccaaaacg acgtatatct
    17881 gaataaatta actttttgtc atttgacaac tcaaaaataa catgccaatg cttacgataa
    17941 ttaggtatca taatatcttc aagttcatct acaatgaaaa aaccgcccgc catacctaaa
    18001 tgactaatta atgtacgttg ttctcgttta ttatctagct gaaaaacgat atatttactt
    18061 cttcgttcta catttgtaat ggtatagcct tccgataaag ttttaaaagt atctaattca
    18121 attcctttta taattgtttc cttgccttga gctttacctt cgattacttt atccgaaaat
    18181 ataacgtgtt caattttttg atttataacg tagggttcaa ttcctctttt tacatgttct
    18241 acttctggta attcgggcat accattaacc tcactttatt ttgcatcata ccaggttgca
    18301 ccataacttg agtctacttt taatggaaca tctaattgca atgcattttc cattatctct
    18361 tctacaaatt cactaaatga atctacttct gacttaggta cttcaaaaat taattcatcg
    18421 tgtacttgta ataatagttt agcttgatat gttgtctctt tcattttttg agcaaattta
    18481 accattgcca gtttaatgat atctgcagca ctgccctgta ttggcgtatt catagcagta
    18541 cgttcagcaa agccgcgtaa attaaagtta cgactcgtaa tatcaggaat atagcgtcga
    18601 cgatgtagca atgtttccac gtaacctaaa gctttggcat cttttacaat atcagacata
    18661 tattgtttta cacctgggaa actagctaaa taatcatcaa tgaatgcttt tgctttttta
    18721 cgagtaatac ctaaactttg acttaaacca taatcactta tcccataaac aattccaaag
    18781 ttaactgctt ttgcttgacg acgcattaaa ctatcgactt gatcagcttc tacaccaaat
    18841 actttcatag cagttgctgt atgaatatca tcgccgttga taaatgcttc tttcatactc
    18901 tcatcttgtg taatgtgtgc taatacacgc aattcaattt gagaataatc tgctgataat
    18961 ataacgctat cttttgaagt tggtttaaag gcttttctta ttttacgccc ttcttcaagt
    19021 ctaaccggaa tattttgtaa attaggatct acacttgata aacgtccagt ttgcgctaaa
    19081 gtttgattaa aacgtgtatg gatacgttga tcatcactaa ttaccttttg caatccttca
    19141 acataagtag actgtaactt agataattga cgatattcta aaatataatc aatgatagga
    19201 tgttcacctt gcaattgctc taagacatct acagcagtag aatatcctgt tttcgtcttt
    19261 ttaataacag gtaattgtaa tgtctcaaac aatacaacac ctaattgctt aggagaattt
    19321 atattaaaat cttcaccagc tgcatcatgg atatttcgaa tcaagacgtc taatttttct
    19381 tgaatttctt tttccatttc ttctaaatca tgaacatctg taaatatacc aatttcttcc
    19441 atttcactta aaatcttagc tagcggtagc tctaaatcag ccaagagttc tacctgattg
    19501 tattcttcta attgtttatc catatttggt ttcgcaaagt aaattgcatc agtaatagaa
    19561 gcaacatatg gatttaaaac atcatcttca ggtaccttaa atttcttacc ttttccatat
    19621 atactcacat cgtctttcac aaaactttga ccgtacaatg aaacaactga ttgaacatca
    19681 ctaatcgtac gagatggatc aattatataa ctggccaaca taatatcgaa agaaatattt
    19741 tgaatatcaa tccccaatct atgtgatgct acatatgttt ttttagcatc atatacgact
    19801 tttttcgaat tcggattttc taaccatgaa actagttcga cataattatt tatgtcatcc
    19861 gcattaatta caatatgttt ctcacctgta aataaagaga attttaaaat attatttcgc
    19921 aaatagttac caccgtctaa ttcgaaatgg atggccgctt ctttcaatga agtaaaatca
    19981 atattatcaa aagacgtttc aatttcaaat gtcttttcta ttgcatcttc aacgcttgct
    20041 gattgatcaa tgtcagccaa caattgtttg aattctaact tcttaaacaa ttcgattttt
    20101 tcttgttgtt catcttgatg agtcattaac gtatcttcaa gttttacttc aatcggacta
    20161 tctacattaa tcgttgctaa ttctttactc attaatgcat cttctttgct attttgaagt
    20221 ttttctttta actttttacc tgaaatttca tctaaatgtt catagacacc ttctactgtg
    20281 tcaaattggt ttagcaattt tattgctgtt ttctctccaa cacctgcaac acctggtata
    20341 ttatcagaag tatctcccat taatcctttc atatcaataa tttgattagg tgttaacccg
    20401 ttgtattttt ccgcaataaa gtcaggtgta taatgatcaa catcagtaac acctttttta
    20461 gtgtaataaa tggttacatt atccgttgca agttgtgtta aatctcggtc tcccgtaata
    20521 ataattgtct gaaagcccgc tttatctgct tctttactta aagttccgat aatatcatct
    20581 gcctcatagt tatctaattc ataacgttta atatgataag catctaataa ttggcgaata
    20641 taaggaaatt gctcacttag ttcaggcggc gttttctggc gtccaccttt atattcacta
    20701 tatttttcat gtctgaaagt cgttttacct gcatcaaacg ctactaaaaa atgatttggc
    20761 ttttcttctt ttaaaatctt ctctagtaac attgcaaaac catatactgc attggtatga
    20821 atgcctgctt tgtttgataa caaaggtaat gcataaaaag ctctaaaact taagctatta
    20881 ccatcgatta atactaattt attcacaatt ttaacctcca gaactaattt atatatattt
    20941 tagcatattc gactgtgatg tcgtaattaa agaaactatc tcatagagta tttatacacg
    21001 acctaatttc aaataaaaca agtgcctata tagacttagc taaaaaataa aaaagacata
    21061 atcatgattg ttcttgatga gcactccata aagtcaggtt aaaagtccaa cttttgagat
    21121 gcacattatc atttcatgat tatgtctact ttttaatatt tttatgctgc tattttaaat
    21181 ttttataggt aagaataagt tatgaatcgt ctgaaccaaa tggaatatat ttaactttgg
    21241 tcattttcgg taattcttca taattattaa accactcttg gtaattttta ttgattggtg
    21301 acggatgatg catataatgt tcaaacggca atgattccac tgtataaacc tcttgtcgtg
    21361 gatgatcttt aatttgttgt tttaataact gaactctttt ttcatgttca taatgaacat
    21421 aaataaatgc actaagatat atcacagcta aaactagtga tgcaccttta ataaaattaa
    21481 cattgattga cttatatttc cgaaattctt ttaataaaat aattaaaatg attacatgta
    21541 tcgtataaac aatcaaaaaa ttacctggtt ctattggagt aacaatgact agtgtcgacg
    21601 ccgaaacaca tattgcaata agtaaggaat ataaagtgat ttgtgtttta cgatcattaa
    21661 tagacaaata aatacctaca aatatcgaaa acgcaaagta accacataca attacgttca
    21721 caaaaccaac caatccaata tctgtatttt tatttaataa aaactgattg taaaatagta
    21781 aataataaag cggtaaagtg ataaatccta tcataatgat acgacgcttt aatattgtta
    21841 aatgtacata ccgatcactt ctaagcagta gataaataat aagcgctgca atcatactta
    21901 aaattataat ctgactgaaa atgacaccat atggaagtga cgttgatatc atttctgcaa
    21961 attttgaaaa gataccttga ttatttgaaa cttgttgata ttctgatcct tcaaataaaa
    22021 ttttgcgata atttggattg gaaaacataa tgattgtacc tatagtagca atcataaatg
    22081 aaaacaataa tttataatta agtgttcgat ttgcagtaaa ttcatacaga aaagctaaaa
    22141 ttaaaatgat acagtggaat aatgtcacat tctccataaa taattgaccg aagaagcata
    22201 aaacatagaa tagtacagta acacttaccg gctgcttttc tttatatata attgcattaa
    22261 tacaataata aataataaag agtgaaatta gtgttgatgt cgcataatta taaaatcctg
    22321 caaaccagcc atatgtatct gcataaatag cacttggtaa aattaacatt aaagaaaatg
    22381 ctaacaaata atagcttgtc cacgctttac aacgtgtaat gtgcataatc atccaaatga
    22441 tgcccatact aatgaggcca tatgaaagcc aacgtaacca gcttacatgt acagctataa
    22501 tttcaaagat atttccgata tagcgaccat taagtgatgc aaatcctact tttaaaatat
    22561 cagtattata attgctaaac cattgtaaat catcgtgcat gagtggtagt aagataccca
    22621 taaaagtata aaacaataat atcgcaatta atatcaaagt tgtcttgtgt aattgaattg
    22681 ttttcacttt gctaatcctc aaatctagtt aaattttcct caacttgtag gtcgaaaaat
    22741 taattcaata tttttaatgt atttctaatt ttcacctatg catgtttcct caatcaaatt
    22801 agataaacaa ggtatttaat attactttca acaatttatc taaatcgccc ctcgtctttt
    22861 tctatgacga atgattacac ttgtctattc aagtgtttaa attaagattt atttccgttc
    22921 aaataaaact tattactttt taattaagca tctatttcaa gcgcgttact aaaatttctt
    22981 tattataata aatatattat aaaattgctt gattcacagc aatctattac ttataactta
    23041 caaatcgaca tatacaaatg acaaaattga caaccgaatt tattccactt cattaactct
    23101 aatttaatac caatagagat ttgaacgaaa taaaaaagca gcaacaactt cgtgaaaaat
    23161 acacgcgctg ttaccacttt aatttttatt ctttataatc ttttagaata actttgaacg
    23221 ttgagccttt gccaacttga ctattcactt caatattgcc ttggtgggcc tctactatat
    23281 gctttgtaat tgataatcca agacctgtcc caccagaatc tctacttcgt gctttatcta
    23341 ctctataaaa tctttcaaaa atacgttgtt ggtcttctaa tttaatacct ataccaaaat
    23401 cttgtacttc gaaaatgaca cgaaagtcat ctcgatacac acgaacgtta atatctccat
    23461 cttcataaga atagttaatt gcattcgtta acaaattcgt gataacttga gcaattttac
    23521 tttcttgtgc tttaacaatg acatcttttt caatatcagt atgaatggaa atattttttt
    23581 gattggcttg agtcatcata ttatcaataa tacgccgcgt taaatctgat aaattcatat
    23641 agtctgtatc taactctgta tgttgctcaa tatgcgataa atctaacaga tctgttacta
    23701 aagattctat tcgatttgat tcttttaaaa ttatgtttaa aaacatgtct aatgattccg
    23761 catcattttt tgcaccatca ataagcgttt cagcaaaccc cttaattgaa gtaataggtg
    23821 tttttaattc atgtgaaaca tttgctacaa attcacgtct tagattttca agttgtttca
    23881 gatttgttat atcatgcatc acaactaaaa tcccttgcaa acttttttga gacctagtta
    23941 aaatcggaac gcatgaaata tcaaagtact tggcatggac ttggtttatt gcaacttcca
    24001 attgttcata aataggtttt tcaactttaa aactttctaa aattaattgc tcaatttcag
    24061 tattaacata gccgtgatag cctacttgtt caatattatg cgagatgttg aactgttcat
    24121 aatacgcttt atttgcaaca acgatttttc catttcgatc tatcattaaa atagcacttg
    24181 gaatattttc aatcgttgtt tttaaacggt tggattgaat tttttgctca ttattaagct
    24241 tttgaaggcg tcgtgctaaa tcattggtag acacaaaaag cgctttagtt tctacaacat
    24301 tactttcagg tacacgtatg tgataataac catttgccaa caattgtgtt gcataagtaa
    24361 cttcttgaat gggacggatt aatgtacgct taaaactacg gcttgcaaaa tacagacaaa
    24421 tgagtacaac taaacatgtc aaaataagat atttccacaa cgtccaatgc atttctgtaa
    24481 tatcgttatt gtaaccttta atccatacat gataaccgtt aaccttctta ttaaaaataa
    24541 aaacgtccct tttttgaata tagctatcac tatttgggat agctttcagt ttgccactac
    24601 taaacacctt tttatcatgc ttagttatca ataaatctat attttgctgt tttacaattt
    24661 ttttaacttt atcaatctca ttatcttgga ctaaataaat atatgatctt gcatctgttg
    24721 ctagagcttg ttcgtgtttt tctgataaaa catatgtgat ggaagcgtga ataataatgc
    24781 ctaatgtaac aaaactgata attaatatac tgcttatcaa taacattaag cggtggtgaa
    24841 acttcatcat tgttctttag gtctttccaa tttatagcct aagccacgca cagttttaat
    24901 aagttgtggc ttcttaggat tatcttctaa tttatctctt aaatgactga tatgtacatc
    24961 aacaattctt gagtctcctg caaattcata attccatacc gtatttaaca tatgctctct
    25021 cgtaatgact ctgccttgtc tttctatcaa ataaagcaag agttcaaatt ctttcggtgt
    25081 tagctccaat aactcattat gcttatatac ttcaaaataa tcgggtctaa tacgtatcga
    25141 tccgatggta atatcatcgt caatctcttc tacttctttt acaaattgag aacgtcttaa
    25201 aatggctttc acacgggcaa caacttctct aggtgaaaaa ggcttagtca tatagtcatc
    25261 ggcacctaat tctaaaccta atacccgatc aaattcatca tttttcgccg ttaacattaa
    25321 tatagggact aaatttttat ttgttcttac agtcttacat acgtcaatgc catctttttt
    25381 aggtagcata acatctaaaa taattaaatc tggctgttca ctttctacct tttctaaagc
    25441 ctcatcacca tcaaatgcga caacaacttc ataaccagct gtttctaagt tatatttaag
    25501 taatgttacg attgaatgtt cgtcatctac taccaacact ttttgcgaca tggtatgcct
    25561 ccctaactta taattatatt tacattataa ccgaactatt tataaaaata acatcctaca
    25621 cattatcttt acacattttt tacattactt tacatataaa taaaatactt cttatatttc
    25681 cttctatcat ttgcatgact tactctggga caacgaaata aattctgtaa aaatagcatt
    25741 tctatcccac tacctatgca tgagttttac tcatttattc ctaagcttat gtacatattc
    25801 gctttgtcta atgtgtaaga aacactacat aatcaatcat tggtgactct ttattatttc
    25861 tatcctgttg ccaacttcaa ttcatttaaa aaggcgaacc tagcaattaa agttgctaga
    25921 ctcacctaat tttattttgc ttattttaaa tttttaatca attcatctgc aaatgctgat
    25981 gtagaaactt cttcagcacc atccattaaa cgggcaaagt cataagtaac aacttttgaa
    26041 gcaattgtat cttcaattga atctgtaatc ttatctgccg cttcttgcca tcctaaatgt
    26101 tctaacatta atacagaact taaaattact gaagatggat tcactttatt taaacctgca
    26161 tattttggag ctgtaccatg tgttgcttca aaaatagcat gacctgtttc ataattaatg
    26221 tttgcacctg gcgcaatacc aataccacca acttgtgcag ctaaagcatc tgaaatatag
    26281 tcaccattca agttcatagt tgctacaaca tcatgctcag ctggacgagt taaaatttgt
    26341 tgtaagaaaa tgtcagcaat agaatcttta atgataatct tgccttcttt ttcagctttt
    26401 tcttgagcag cattagcagc atctctgcct tcattttcaa caatttcgtc atattgttgc
    26461 caagtgaata cttgatcacc aaattcagat aatgctaaat cgtaacccca ctgcttaaat
    26521 gagccttctg taaatttcat aatattacct ttatgaacta aagtaactga tttacggtta
    26581 ttatcgatag catattgtat agctgctcta actaatcgct cagttccttc tttagaaact
    26641 ggtttaatac caatacctga agtttctggg aatcgaatgt ttgtcgcacc catttcgttt
    26701 tgtaagaagt caattacctt tttaacttct gttgtacctt ctttaaattc aataccagca
    26761 taaatgtctt cagtattttc acggaaaata accatatcaa catcttgtgg acgtttaaca
    26821 ggtgatggta ctcctttaaa ccaacgtacc ggtcttaagc aagtaaataa atctaattct
    26881 tggcgtaaag ccacatttaa tgatctaata ccaccaccaa ttggtgttgt taaaggtcct
    26941 ttaacagcaa ttaaatattc tttaattgta tcaagtgttt cttgaggtaa ccattcacca
    27001 gttgtatcaa atgctttttg gccagctagc acttctttcc attcaatgcg tttttcgcca
    27061 ttataggctt tctcaacagc agcatctata actcggcttg ctgccttcca aatatccggt
    27121 ccaattccat caccgataat aaatgggata attggttcat taggtacgtt taatccttca
    27181 gttccttgag taattttttc tgcagtcata gttattttac ctccaaattt tacatcttca
    27241 tttttaattt gtattgatta ttttctttct tcaagcggga tatacttacg attcgtttcg
    27301 ccaatatatt tcgctctagg acgcataatt ctattatctt tatattgttc taaaatatga
    27361 gcaatccatc ctgcagaacg acttacagca aagattggcg tgaataagtc atgaggtatt
    27421 tccatacagt gataaacact cgcactataa aaatcaacat taggaattaa tcctttttct
    27481 tctgccatac gtttttccat tttcactgac atttcaaata attcttcacg accagcgtct
    27541 ttcgtaattt gacggctcat ttctcttaaa tatttcgctc taggatcacc atctttatat
    27601 acacgatgac cgaagcccat tactttatct ttattagcaa atttttcatc taagtaagca
    27661 tcaacatttt caattgaccc aatctcagat aacatcgtca taacttgttc gtttgcacca
    27721 ccatgtaatg gccctttcag agaacctacg gctgctacaa tacctgagta catatctgac
    27781 aatgatgata ccgcacaacg tgccgtaaat gcagatgcgt tcaactcatg atcagcgtgt
    27841 aaaataagtg ctttattgaa ggcttctact tctatatctg ttggtaattc cccacgtaac
    27901 atatatagga agtttgccgc ataacttaag tcaggattag gcttaagtgg ttctttatct
    27961 tgtcttactc gagcaaacgc tgtaactaat gatgctactt tagcctgtat acgcattgct
    28021 ctttcataac gattttcatc tgattcattt tcagcatcag gatcgaaatg tgcaatatat
    28081 gataatgacg tacgtaatgc tgtcattgga tgcacgtgat ctgtaacata ctcctcaaaa
    28141 tgtgtataca cacgaggatt taatgtcatg tattgattta atttcccttt tagatgagca
    28201 agctcttctt cgtttggcaa tctatagttc cataatagga aaataacttc ttcaaattgc
    28261 gcattttcag ctagatcatc aatatcatag ccggcataag tcaattgact ttcaataatt
    28321 gaacttattt tagtctccgc tgcgataacc ccttctaaac ctctttgtaa ttctgccatg
    28381 ataaatttcc cctttactgt ttctttatga aatggctttc aactaatatt atagctaaac
    28441 gcatttcgtt tgtgaaatat aaaaactggc attatatgtg caacaatttt tttgtaaatc
    28501 tatgtaaatg tcacagaata gccacaaata gcaccattaa agctttttca agatgtgaat
    28561 aatccatgaa ttttacaata gaattaaatt ttcaattggt tattatatat gattataaaa
    28621 accaaagtat attaacatgg aattttacac atttttaccg aaaataattg tatatatctc
    28681 aatataagtg cataataaac atatctatat ttaagggaga tgtattatgg ctgaaaaatt
    28741 acaaagggaa ctgagcaatc gccacataca attaattgca attggcggtg caattggtac
    28801 aggcttattc ttaggtgctg gtcaaacgat tgcattaacc ggcccttcaa ttctattaac
    28861 atacatcatt ataggattta tgttatttat gtttatgcga ggtttaggag aaatcattat
    28921 acagaatact gaatttaaat ctttcgcaga tgtaaccaat acatatattg ggccttttgc
    28981 aggatttgtt accggatgga catactggtt ctgttggatt attacaggta tggctgaagt
    29041 aacggctgtg gcaaaatatg ttagcttttg gttcccagaa attccaaact ggataagtgc
    29101 actattttgt gtactgttat taatgtcatt caacctactt agcgcaagac ttttcggaga
    29161 attagaattt tggttctcta tcattaaaat agcgacaatt attggtttaa tagtagttgg
    29221 tttcgtcatg attctatttg catttaaaac tcaattcggg catgccagtt tcacaaatct
    29281 atatgaacac ggcatattcg ctaaaggtgc ttctggattc tttatgtctt tccaaatggc
    29341 actattctca tttgtaggaa ttgaaatgat tggtgttaca gctggggaaa caaaagatcc
    29401 agttaaaaca attccaaaag caattaacag tgtacccatt agaattttaa tattttacgt
    29461 tggggcgtta gcggttatca tgtctattat cccttggcag caagttgatc ctgataacag
    29521 tccattcgta aaattattcg cattgatcgg aattccgttt gctgcgggct tgattaattt
    29581 tgtagtatta accgctgctg cttcatcatg taacagtggt atattctcaa atagccgtat
    29641 gcttttcggt ttatcaagtc aacaacaagc acctccgaac ttttctaaga cgaataaata
    29701 tggcgttcca catgttgcaa tctttgcttc atcagcatta ttacttgtgg cagcattact
    29761 aaactatatt ttcccagatg cgacaaaagt atttacgtat gtgactacca tctctacagt
    29821 gttattttta gttgtatggg gtctgattat cattgcatat atcaattata gtcgtaaaaa
    29881 cccagatcta cataaaaatg ctacgtacaa actattaggt ggcaaatata tgggctactt
    29941 aatatttgta ttcttcattt ttgtgttcgg gttattattt attaatgttg atacaagacg
    30001 tgcaatttat tttattccga tttggtttat acttttagca tttatgtact taagatataa
    30061 acgtatcgct gctaaatcaa ataaataaca acaagttt
//
LOCUS       DAKTLH010000017        29822 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.017, whole genome shotgun sequence.
ACCESSION   DAKTLH010000017 DAKTLH010000000
VERSION     DAKTLH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 29822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29822
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.017"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            97..1383
                     /gene="rseP"
                     /locus_tag="O0394_000692"
     CDS             97..1383
                     /gene="rseP"
                     /locus_tag="O0394_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCY0498792.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTEKKLTK
                     VSSETKYVLGFQPASEHTLFKPIVFGFKSFLIGSTYIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            1403..3106
                     /locus_tag="O0394_000693"
     CDS             1403..3106
                     /locus_tag="O0394_000693"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCY0498793.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQSLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            3370..7680
                     /locus_tag="O0394_000694"
     CDS             3370..7680
                     /locus_tag="O0394_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCY0498794.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            7970..8437
                     /gene="rimP"
                     /locus_tag="O0394_000695"
     CDS             7970..8437
                     /gene="rimP"
                     /locus_tag="O0394_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCY0498795.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            8458..9633
                     /gene="nusA"
                     /locus_tag="O0394_000696"
     CDS             8458..9633
                     /gene="nusA"
                     /locus_tag="O0394_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCY0498796.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDVALEDADTTESTEEVNDVSVETNVEKESE"
     gene            9654..9938
                     /locus_tag="O0394_000697"
     CDS             9654..9938
                     /locus_tag="O0394_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCY0498797.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            9935..10252
                     /locus_tag="O0394_000698"
     CDS             9935..10252
                     /locus_tag="O0394_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HCY0498798.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            10257..12374
                     /gene="infB"
                     /locus_tag="O0394_000699"
     CDS             10257..12374
                     /gene="infB"
                     /locus_tag="O0394_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCY0498799.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIIQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            12760..13110
                     /gene="rbfA"
                     /locus_tag="O0394_000700"
     CDS             12760..13110
                     /gene="rbfA"
                     /locus_tag="O0394_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCY0498800.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            13279..14196
                     /gene="truB"
                     /locus_tag="O0394_000701"
     CDS             13279..14196
                     /gene="truB"
                     /locus_tag="O0394_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCY0498801.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            14211..15182
                     /gene="ribF"
                     /locus_tag="O0394_000702"
     CDS             14211..15182
                     /gene="ribF"
                     /locus_tag="O0394_000702"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCY0498802.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            15297..15566
                     /gene="rpsO"
                     /locus_tag="O0394_000703"
     CDS             15297..15566
                     /gene="rpsO"
                     /locus_tag="O0394_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCY0498803.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
     gene            15936..18032
                     /gene="pnp"
                     /locus_tag="O0394_000704"
     CDS             15936..18032
                     /gene="pnp"
                     /locus_tag="O0394_000704"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCY0498804.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            18268..19941
                     /locus_tag="O0394_000705"
     CDS             18268..19941
                     /locus_tag="O0394_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCY0498805.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            20197..22566
                     /locus_tag="O0394_000706"
     CDS             20197..22566
                     /locus_tag="O0394_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCY0498806.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHREVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            22571..23284
                     /locus_tag="O0394_000707"
     CDS             22571..23284
                     /locus_tag="O0394_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0498807.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNESDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            23315..24580
                     /locus_tag="O0394_000708"
     CDS             23315..24580
                     /locus_tag="O0394_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCY0498808.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            24580..25866
                     /locus_tag="O0394_000709"
     CDS             24580..25866
                     /locus_tag="O0394_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCY0498809.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFDTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVK
                     QHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            25866..26570
                     /locus_tag="O0394_000710"
     CDS             25866..26570
                     /locus_tag="O0394_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCY0498810.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            26675..27502
                     /locus_tag="O0394_000711"
     CDS             26675..27502
                     /locus_tag="O0394_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HCY0498811.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            27521..27913
                     /locus_tag="O0394_000712"
     CDS             27521..27913
                     /locus_tag="O0394_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0498812.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            27947..28525
                     /gene="pgsA"
                     /locus_tag="O0394_000713"
     CDS             27947..28525
                     /gene="pgsA"
                     /locus_tag="O0394_000713"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCY0498813.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFKQK"
     gene            28752..>29822
                     /locus_tag="O0394_000714"
     CDS             28752..>29822
                     /locus_tag="O0394_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HCY0498814.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLTNHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNFEFKTEQFKMS"
BASE COUNT        10895 a         4014 c         5812 g         9101 t
ORIGIN      
        1 tgtgatttgt cagtatcata ttataatgac ttatgttacg tatacagcaa tcatttttaa
       61 aataaaagaa atttataaac aatcgaggtg tagcgagtga gctatttagt tacaataatt
      121 gcatttatta ttgtttttgg tgtactagta actgttcatg aatatggcca tatgtttttt
      181 gcgaaaagag caggcattat gtgtccagaa tttgcgatcg gtatggggcc aaaaattttt
      241 agttttagaa aaaatgaaac actttacact attaggttat tgcctgttgg tggatatgtt
      301 cgtatggcag gagatggctt agaagagcca ccagtcgagc ccggtatgaa cgttaaaatt
      361 aaacttaatg aagaaaatga aataacacat atcatattag atgatcatca taagtttcaa
      421 caaattgaag cgatcgaagt taaaaaatgt gattttaagg atgacttatt catagaaggt
      481 atcactgctt atgataatga aagacatcat tttaaaattg ctagaaagtc tttctttgtt
      541 gaaaatggta gcttagttca aattgctccg agagacagac aatttgcaca taaaaagcca
      601 tggccgaaat ttttaacatt atttgcggga ccgttattta actttatatt agctttagtc
      661 ctatttattg gtcttgcata ttatcaaggc acgcctacgt ctactgtaga acaagtcgca
      721 gataagtatc cagctcaaca agcaggatta caaaaaggtg ataagatcgt ccaaattggc
      781 aaatataaaa tatctgaatt tgatgatgtt gataaggcgt tagataaagt taaagataat
      841 aagacgactg ttaaatttga acgtgatggt aaaacaaagt cagttgaatt aacacctaaa
      901 aagactgaaa aaaaactgac taaagtaagt tcagagacga agtatgttct cggattccaa
      961 ccagcgagtg aacatacact ttttaaacca attgtattcg gatttaaaag ctttttaatc
     1021 ggtagtactt atatttttac agctgtagta ggtatgttgg ctagtatatt tacgggcgga
     1081 ttctcatttg atatgttaaa tggtccggtt ggtatttatc ataacgtcga ctcagttgtt
     1141 aaagcgggta tcattagctt aattggttac actgcgttat taagtgtaaa cttaggtatt
     1201 atgaatttaa ttcctattcc tgcactagac ggtggtcgta ttttatttgt tatatatgaa
     1261 gcgattttca gaaaaccagt taataaaaaa gcggaaacaa cgattattgc tattggtgcc
     1321 attttcatgg tcgttataat gatattagta acgtggaatg atattcgacg atatttctta
     1381 taatttagga ggataaataa ttatgaagca atccaaagtt tttataccaa cgatgcgtga
     1441 cgtgccatca gaagcagaag cacaaagtca tcgtttatta ttgaaatcgg gtttgataaa
     1501 acaaagtaca agtgggattt atagttattt accgctagca acacgtgtgt taaataatat
     1561 tactgcaatt gtgcgacaag aaatggaacg tatcgattct gttgaaattt taatgccagc
     1621 gttacaacaa gctgaattat gggaagaatc aggacgttgg ggtgcatatg gcccagaatt
     1681 aatgcgttta caagatagac atggaagaca atttgcatta ggtccaacac atgaagaatt
     1741 agttacatca atagtaagaa atgaattgaa atcatacaaa caattaccga tgacattatt
     1801 ccaaattcaa tctaaattcc gtgatgaaaa gagaccacgt tttggtttac ttcgtgggcg
     1861 tgaatttatt atgaaagatg catattcatt ccatgctgac gaggcatcat tagatcaaac
     1921 gtatcaagat atgtatcaag cgtatagccg tatttttgag agagttggca ttaacgcaag
     1981 accagtagtt gcagattcag gtgctatagg cggtagccat acacatgaat ttatggcatt
     2041 aagtgctatc ggtgaggata caatcgttta cagtaaagaa agtgattatg ctgctaacat
     2101 cgaaaaagca gaagtcgttt acgaaccaaa tcataagcat actactgtgc aacctttaga
     2161 aaaaattgaa acaccaaatg ttaagactgc gcaagaattg gcagacttct taggtagacc
     2221 agtagatgaa atcgttaaaa cgatgatttt caaagttgat ggcgaatata ttatggtttt
     2281 agtgcgtggc catcatgaaa ttaatgacat taaattaaaa tcttatttcg gcacagataa
     2341 tattgaatta gcaacacaag acgaaattgt taatttagtt ggtgcaaatc ctggttcact
     2401 aggtcctgta attgataaag aaatcaaaat ttatgcagat aattttgtgc aagatttaaa
     2461 taatttagtt gtcggtgcta acgaagatgg ttatcactta attaatgtaa atgtaggtag
     2521 agacttcaac gttgatgaat atggcgattt ccgttttatt ttagaaggcg aaaagttaag
     2581 tgatggttca ggcgttgcac attttgctga aggtattgaa gttggtcaag tattcaaatt
     2641 gggtactaag tattcagaat caatgaatgc tacattctta gataaccaag gaaaagctca
     2701 atctttaatt atgggttgtt acggaattgg aatttctaga acgctaagtg cgattgttga
     2761 acaaaatcac gatgataatg gaattgtttg gcctaaatca gttactccgt ttgatttaca
     2821 tttaatttct attaatccta agaaagatga tcaacgagaa ctagcagatg cactatatgc
     2881 tgaatttaat actaaatttg atgtgttgta cgatgatcgt caggaacgtg caggtgttaa
     2941 atttaatgat gccgatttaa ttggtttacc actgcgaatt gttgttggta aacgtgcatc
     3001 ggaaggtatt gtagaagtta aagaacgttt aacaggtgat agcgaagaag ttcacattga
     3061 tgacttaatg actgtcatta caaataaata tgataactta aaataattaa gatcgaatga
     3121 attataagag taggaaaaag ctgaaagaaa tctgatgctt atgtcctgct cttattattt
     3181 ttgatataat gattattcga tgaaaaatga ctgaagacat agtataatta aagataaatt
     3241 tgttttaaca atataatgat tagccaaata taaagcattt aattttctat cattactatg
     3301 ctcacataat ctaaatattg ttcgaacacg taaaagtaat ttctatttaa ggtggtaatt
     3361 gtcttggcaa tgacagagca acaaaaattt aaagtgcttg ctgatcaaat taaaatttca
     3421 aatcaattag atgctgaaat tttaaattca ggtgaactga cacgtataga tgtttctaac
     3481 aaaaacagaa catgggaatt tcatattaca ttaccacaat tcttagctca tgaagattat
     3541 ttattattta taaatgcaat agagcaagag tttaaagata tcgccaacgt tacatgtcgt
     3601 tttacggtaa caaatggcac gaatcaagat gaacatgcaa ttaaatactt tgggcactgt
     3661 attgaccaaa cagctttatc tccaaaagtt aaaggtcaat tgaaacagaa aaagcttatt
     3721 atgtctggaa aagtattaaa agtaatggta tcaaatgaca ttgaacgtaa tcattttgat
     3781 aaggcatgta atggaagtct tatcaaagcg tttagaaatt gtggttttga tatcgataaa
     3841 atcatattcg aaacaaatga taatgatcaa gaacaaaact tagcttcttt agaagcacat
     3901 attcaagaag aagacgaaca aagtgcacga ttggcaacag agaaacttga aaaaatgaaa
     3961 gctgaaaaag cgaaacaaca agataacaac gaaagtgctg tcgataagtg tcaaattggt
     4021 aagccgattc aaattgaaaa tattaaacca attgaatcta ttattgagga agagtttaaa
     4081 gttgcaatag agggtgtcat ttttgatata aacttaaaag aacttaaaag tggtcgccat
     4141 atcgtagaaa ttaaagtgac tgactatacg gactctttag ttttaaaaat gtttactcgt
     4201 aaaaacaaag atgatttaga acattttaaa gcgctaagtg ttggtaaatg ggttagggct
     4261 caaggtcgta ttgaagaaga tacatttatt agagatttag ttatgatgat gtctgatatt
     4321 gaagagatta aaaaagcgac aaaaaaagat aaggctgaag aaaagcgtgt agaattccac
     4381 ttgcatactg caatgagcca aatggatggt atacccaata ttggtgcgta tgttaaacag
     4441 gcagcagact ggggacatcc agccattgcg gttacagacc ataatgttgt gcaagcattt
     4501 ccagatgctc acgcagcagc ggaaaaacat ggcattaaaa tgatatacgg tatggaaggt
     4561 atgttagttg atgatggtgt tccgattgca tacaaaccac aagatgtcgt attaaaagat
     4621 gctacttatg ttgtgttcga cgttgagaca actggtttat caaatcagta tgataaaatc
     4681 atcgagcttg cagctgtgaa agttcataac ggtgaaatca tcgataagtt tgaaaggttt
     4741 agtaatccgc atgaacgatt atcggaaacg attatcaatt tgacgcatat tactgatgat
     4801 atgttagtag atgcccctga gattgaagaa gtacttacag agtttaaaga atgggttggc
     4861 gatgcgatat tcgtagcgca taatgcttcg tttgatatgg gcttcatcga tacgggatat
     4921 gaacgtcttg ggtttggacc atcaacgaat ggtgttatcg atactttaga attatctcgt
     4981 acgattaata ctgaatatgg taaacatggt ttgaatttct tggctaaaaa atatggcgta
     5041 gaattaacgc aacatcaccg tgccatttat gatacagaag caacagctta cattttcata
     5101 aaaatggttc aacaaatgaa agaattaggc gtattaaatc ataacgaaat caacaaaaaa
     5161 ctcagtaatg aagatgcata taaacgtgca agacctagtc atgtcacatt aattgtacaa
     5221 aaccaacaag gtcttaaaaa tctatttaaa attgtaagtg catcattggt gaagtatttc
     5281 taccgtacac ctcgaattcc acgttcattg ttagatgaat atcgtgaggg attattggta
     5341 ggtacagcgt gtgatgaagg tgaattattt acggcagtta tgcagaagga ccagagtcaa
     5401 gttgaaaaaa ttgccaaata ttatgatttt attgaaattc aaccaccggc actttatcaa
     5461 gatttaattg atagagagct tattagagat actgaaacat tacatgaaat ttatcaacgt
     5521 ttaatacatg caggtgacac agcgggtata cctgttattg cgacaggaaa tgcacactat
     5581 ttgtttgaac atgatggtat cgcacgtaaa attttaatag catcacaacc cggcaatcca
     5641 cttaatcgct caactttacc ggaagcacat tttagaacta cagatgaaat gttaaacgag
     5701 tttcattttt taggtgaaga aaaagcgcat gaaattgttg tgaaaaatac aaacgaatta
     5761 gcagatcgaa ttgaacgtgt tgttcctatt aaagatgaat tatacacacc gcgtatggaa
     5821 ggtgctaacg aagaaattag agaactaagt tatgcaaatg cgcgtaaact gtatggtgaa
     5881 gacctgcctc aaatcgtaat tgatcgatta gaaaaagaat taaaaagtat tatcggtaat
     5941 ggatttgcgg taatttactt aatttcgcaa cgtttagtta aaaaatcatt agatgatgga
     6001 tacttagttg gttcccgtgg ttcagtaggt tctagttttg tagcgacaat gactgagatt
     6061 actgaagtaa acccgttacc gccacactat atttgtccga actgtaaaac gagtgaattt
     6121 ttcaatgatg gttcagtagg atcaggattt gatttacctg ataagacgtg tgaaacttgt
     6181 ggagcgccac ttattaaaga aggacaagat attccgtttg aaacattttt aggatttaag
     6241 ggagataaag ttcctgatat cgacttaaac tttagtggtg aatatcaacc gaatgcccat
     6301 aactacacaa aagtattatt tggtgaggat aaagtattcc gtgcaggtac aattggtact
     6361 gttgctgaaa agactgcttt tggttatgtt aaaggttatt tgaatgatca aggtatccac
     6421 aaaagaggtg ctgaaataga tcgactcgtt aaaggatgta caggtgttaa acgtacaact
     6481 ggacagcatc cagggggtat tattgtagta cctgattaca tggatattta tgattttacg
     6541 ccgatacaat atcctgccga tgatcaaaat tcagcatgga tgacgacaca ttttgatttc
     6601 cattctattc atgataatgt attaaaactt gatatacttg gacacgatga tccaacaatg
     6661 attcgtatgc ttcaagattt atcaggaatt gatccaaaaa caatacctgt agatgataaa
     6721 gaagttatgc agatatttag tacacctgaa agtttgggtg ttactgaaga tgaaatttta
     6781 tgtaaaacag gtacatttgg ggtaccagaa ttcggtacag gattcgtgcg tcaaatgtta
     6841 gaagatacaa agccaacaac attttctgaa ttagttcaaa tctcaggatt atctcatggt
     6901 acagatgtgt ggttaggcaa tgctcaagaa ttaattaaaa ccggtatatg tgatttatca
     6961 agtgtaattg gttgtcgtga tgatatcatg gtttatttaa tgtatgctgg tttagaacca
     7021 tcaatggctt ttaaaataat ggagtcagta cgtaaaggta aaggtttaac tgaagaaatg
     7081 attgaaacga tgaaagaaaa tgaagtgcca gattggtatt tagattcatg tcttaaaatt
     7141 aagtacatgt tccctaaagc ccatgcagca gcatacgttt taatggcagt acgtatcgca
     7201 tatttcaaag tacatcatcc actttattac tatgcatctt actttacaat tcgtgcgtca
     7261 gactttgatt taatcacgat gattaaagat aaaacaagca ttcgaaatac tgtaaaagac
     7321 atgtattctc gctatatgga tctaggtaaa aaagaaaaag acgtattaac agtcttggaa
     7381 attatgaatg aaatggcgca tcgaggttat cgaatgcaac cgattagttt agaaaagagt
     7441 caggcgttcg aatttatcat tgaaggcgat acacttattc cgccgttcat atcagtgcct
     7501 gggcttggcg aaaacgttgc gaaacgaatt gttgaagctc gtgacgatgg cccattttta
     7561 tcaaaagaag atttaaacaa aaaagctgga ttatctcaga aaattattga gtatttagat
     7621 gagttaggct cattaccgaa tttaccagat aaagctcaac tttcgatatt tgatatgtaa
     7681 aatgaaataa tcaaggtatt tatttaatgc gtatggcgta gtcaaagaaa tacaaaattg
     7741 ttgctggaca caaaattatg cccgtatttc ttttcaatgt cttacgagtc tattcaaatg
     7801 taatggtgaa ataaaggaac aaacttttac aagaatctct gattaatagt gaagtcattt
     7861 gtttcaagca taaacttatg ctataattaa gttgcttaaa aattagtgaa ctcaggcaga
     7921 agagtgggag attcccgctc ttttctattt gccaaaaagg gaggcctgta tgagtaaaat
     7981 tacagaacaa gtagaagtga ttgttaaacc aattatggaa gacttgaatt ttgaacttgt
     8041 agacgttgaa tatgtcaaag agggtagaga tcattttctt agaatctcta ttgataaaga
     8101 aggtggcgta gatttaaatg attgtacgct agcttctgaa aaaataagtg aagctatgga
     8161 tgcaaatgat cctattcctg aaatgtatta tttagacgta gcgtcacctg gtgcagaacg
     8221 tccaattaaa aaagaacaag atttccaaaa tgcaataact aaacctgtat ttgtttcttt
     8281 atatgtacca attgaaggtg aaaaggaatg gttaggcatt ttacaagaag tcaataatga
     8341 aacaattgta gtacaagtta aaatcaaagc aagaacgaaa gatatagaga taccgagaga
     8401 caaaatagca aaagcacgtc acgcagttat gatttaacgt gatgaggagg aaaaaacgtg
     8461 tcaagtaatg aattattatt agctactgag tatttagaaa aagaaaagaa gattcctaga
     8521 gcagtattaa ttgatgctat tgaagcagct ttaattactg catacaaaaa gaattatgat
     8581 agtgcaagaa atgtccgtgt ggaattaaat atggatcaag gtactttcaa agttatcgct
     8641 cgtaaagatg ttgttgaaga agtatttgac gacagagatg aagtggattt aagtacagcg
     8701 cttgttaaaa accctgcata tgaaattggt gatatatacg aagaagatgt aacacctaaa
     8761 gattttggtc gtgtaggtgc tcaagcagcg aaacaagcag taatgcaacg tcttcgtgat
     8821 gctgaacgtg aaattttatt tgaagaattt atagacaaag aagaagacat acttactgga
     8881 attattgacc gtgttgacca tcgttatgta tatgtgaatt taggtcgtat cgaagctgtt
     8941 ttatctgaag cagaaagaag tcctaacgaa aaatatattc ctaacgaacg tatcaaagta
     9001 tatgttaaca aagtggaaca aacgacaaaa ggtcctcaaa tctatgtttc tcgtagccat
     9061 ccaggtttat taaaacgttt atttgaacaa gaagttccag aaatttacga tggtactgta
     9121 attgttaaat cagtagcacg tgaagctggc gatcgctcta aaattagtgt cttctctgaa
     9181 aacaatgata tagatgctgt tggtgcatgt gttggtgcta aaggcgcacg tgttgaagct
     9241 gttgttgaag agctaggtgg tgaaaaaatc gacatcgttc aatggaatga agatccaaaa
     9301 gtatttgtaa aaaatgcttt aagcccttct caagttttag aagttattgt tgatgaaaca
     9361 aatcaatcta cagtagttgt tgttcctgat tatcaattgt cattagcgat tggtaaaaga
     9421 ggacaaaacg cacgtctagc tgctaaatta accggctgga aaattgatat taaatcagaa
     9481 acagatgcgc gtgaagcggg tatctatcca gtagttgaag ctgaaaaagt aactgaagaa
     9541 gatgttgctt tagaagatgc tgacacaaca gaatcaaccg aagaggtaaa tgatgtttca
     9601 gttgaaacaa atgtagagaa agaatctgaa taataggttg gagtgaagta tctatgaaaa
     9661 agaaaaaaat tccgatgcga aaatgtattc tttcaaatga aatgcatccc aaaaaagata
     9721 tgattcgtgt tgttgttaat aaagaaggcg aaatctttgc ggatgttact ggaaagaaac
     9781 aaggccgtgg cgcatatgtt tctaaagatg ttgctatggt tgaaaaagca caacaaaaag
     9841 aaattttaga aaaatatttt aaagcatcta aagagcaatt ggatcctgtt tacaaagaaa
     9901 ttattagatt aatttataga gaagagatcc caaaatgagt atagatcaaa tattaaactt
     9961 tttaggatta gcaatgagag ctggtaaagt aaaaacaggt gaatcagtca ttgttaatga
    10021 gattaaaaaa ggaaatttga agctcgttat tgttgcaaat gatgcgtctg ataatacagc
    10081 taaattaatt acagataaat gtaagagtta caaagttcca ttcagaaagt ttggaaatcg
    10141 aaatgaattg ggaatagcac ttggaaaagg tgagcgtgtt aatgtaggga ttactgaccc
    10201 aggctttgct aaaaagttgc tatcaatgat agatgaatat cataaggagt gattatatga
    10261 gtaaacaaag aatttacgaa tatgcgaaag aattaaatct aaagagtaaa gagattatag
    10321 atgagttaaa aagcatgaat attgaggttt caaatcatat gcaagctttg gaagatgacc
    10381 aaattaaagc attagataaa aagttcaaaa aagaacaaaa gaacgacaat aaacaaagca
    10441 ctcaaaataa tcaccaaaaa tcaaacaatc aaaaccaaaa taaagggcaa caaaaagata
    10501 acaaaaagaa tcaacaacaa aataataaag gcaacaaagg caataaaaag aataatagaa
    10561 ataataagaa aaataacaag aataataaac cacaaaatca accagctgct ccaaaagaaa
    10621 taccatcaaa agtgacatat caagaaggta ttacagtagg cgaatttgcg gataaattaa
    10681 atgttgaatc atcagaaatt atcaaaaaat tattcttact tggtattgtt gctaatatca
    10741 atcaatcatt aaatcaagaa acaatcgaat taattgccga tgattatggc gttgaggttg
    10801 aagaagaagt tgtgattaat gaagaagact tatcaatcta tttcgaagac gaaaaagatg
    10861 atccagaggc aattgagaga ccagcagttg taacaattat gggacatgtt gaccatggta
    10921 aaacgacttt attagattca attcgtcata caaaagttac agcaggtgaa gcaggcggaa
    10981 tcactcaaca tattggtgca tatcaaattg aaaacgatgg caaaaaaatc actttcttag
    11041 atacaccggg acatgctgca tttacaacga tgcgtgcgcg tggtgcacaa gtaacagata
    11101 ttactatttt agtagtagca gctgacgatg gtgttatgcc acaaacaatt gaagcaatta
    11161 accatgctaa agaagcagaa gtaccaatta ttgttgcagt aaataaaatt gataaaccaa
    11221 cttcaaatcc tgatcgagtt atgcaagaat taactgaata tggtttaatt cctgaagatt
    11281 ggggcggcga aacaattttc gttccacttt ctgcattaag tggtgatggt atcgacgatt
    11341 tattagaaat gataggatta gttgcagaag ttcaagaact taaagcaaat cctaaaaacc
    11401 gtgctgttgg tacagttatc gaagctgaat tagataaatc acgtggtcct tctgcatcat
    11461 tattagtaca aaacggtaca ttaaatgttg gtgatgcgat tgtagttggt aatacttacg
    11521 gccgtatccg tgcaatggtt aatgacttag gtcaaagaat caaaacggct ggtccatcaa
    11581 cgcctgttga aattacaggt attaatgatg tgccacaagc tggggatcgc tttgttgtat
    11641 ttagtgatga aaaacaagct cgtcgtattg gtgaatcaag acacgaagct agcattatac
    11701 aacaacgtca agaaagtaaa aatgtttcat tagataacct gtttgaacaa atgaaacaag
    11761 gtgaaatgaa agatttaaac gttattatta aaggtgatgt tcaaggttct gttgaagctt
    11821 tagctgcatc attaatgaaa attgatgttg aaggcgtaaa tgttcgtatc attcatacag
    11881 cggttggtgc aattaatgag tcagacgtga cacttgctaa tgcctcaaat ggtattatca
    11941 ttggtttcaa tgttcgtcca gacagtggtg caaaacgtgc tgcagaagct gaaaatgttg
    12001 atatgcgttt acacagagtt atttataatg ttatcgaaga aattgaatca gcgatgaaag
    12061 gtttacttga tccagaattt gaagaacaag ttatcggaca agctgaagtt cgtcaaacat
    12121 tcaaagtttc taaagttggt actattgctg gatgttatgt tactgaaggt aaaattacgc
    12181 gaaatgctgg tgtacgtatt attcgtgatg gtattgttca atatgaaggc gaattagata
    12241 cacttaaacg tttcaaagat gatgctaagg aagttgcaaa aggttatgaa tgtggtatta
    12301 caattgaaaa ctacaatgac cttaaagaag gcgatgttat cgaagcattt gaaatggttg
    12361 aaattaagcg ttaattaaat aaattacaag ctaaaagtat agttaagatt gatatgctcc
    12421 ctataaatat tgcacttttt aagtgtctac tttataggga gcatatttga tactagcttt
    12481 tggtttttta ttagaataga ttacctatta aaagttacgt tatatggaca tgattttgta
    12541 taaaattttg tggtggccta gaatgatttt taatgacaaa atataatgtc gactattatt
    12601 ggaaaatttt ctgttgaaat gcctatctta cggcaaactt tatttgattt tataggctta
    12661 atttattaaa ataacgtgtg agctaaaata attgtttaag cattgttaca ctaaaaaatg
    12721 caaataacaa ttgaacttaa agataaagag gtgacaagaa tgagcagtat gagagcagag
    12781 cgtgttggtg aacaaatgaa gaaggaatta atggatatca tcaacaataa agtcaaagat
    12841 cctcgagttg gttttattac aattacagat gttgttttaa caaatgattt atcgcaggct
    12901 aaagtatttt taactgtatt aggtaacgat aaagaagtag aaaatacatt taaagcactt
    12961 gataaagcaa aaggcttcat taagtctgaa ttaggttcta gaatgcgatt acgtattatg
    13021 ccggaattaa tgtatgaata tgatcaatca atcgaatatg gtaataaaat tgaacgaatg
    13081 attcaagatt tacacaaaca agatagataa tttagtgtta ggtatctgga aaatgtttga
    13141 taatttctta atatcggtat attaacatta aacagttaat acatagatgt gtagaaatag
    13201 ttaacatttt ccagtttttt tatgaataaa tttagttgat acgctattaa aatatatttt
    13261 aaaaaagaag gtgactatat gtataatggg atattaccag tatataaaga gcgcggttta
    13321 acaagtcatg acgttgtatt caaattgcgt aaaatattaa aaactaaaaa aataggtcac
    13381 acgggtacgc ttgatcccga agttgcaggc gtgttaccgg tatgtatagg taatgcaacg
    13441 agagttagtg attatgttat ggatatgggc aaagcttatg aagcaactgt atcgatagga
    13501 agaagtacaa cgactgaaga tcaaacgggt gatacattgg aaacaaaagg tgtacactca
    13561 gcagatttta ataaggacga tattgaccga ttgttagaaa gttttaaagg tatcattgaa
    13621 caaattccgc cgatgtactc atccgtcaaa gtaaatggta aaaaattata tgaatatgcg
    13681 cgtaataatg aaacagttga aagaccaaag cgtaaagtta atattaaaga cattgggcgt
    13741 atatctgaat tagattttaa agaaaatgag tgtcatttta aaatacgcgt catctgtggt
    13801 aaaggtacat atattagaac gctagcaact gatattggtg tgaaattagg ctttccggca
    13861 catatgtcga aattaacacg aatcgagtct ggtggatttg tgttgaaaga tagccttaca
    13921 ttagaacaaa taaaagaact tcatgagcag gattcattgc aaaataaatt gtttccttta
    13981 gaatatggat taaagggttt gccaagcatt aaaattaaag attcgcacat aaaaaaacgt
    14041 attttaaatg ggcagaaatt taataaaaat gaatttgata acaaaattaa agaccaaatt
    14101 gtatttattg atgatgattc agaaaaagta ttagcaattt atatggtaca ccctacaaaa
    14161 gaatcagaaa ttaaacctaa aaaagtcttt aattaaagga gatagaattt atgaaagtca
    14221 tagaagtgac acatcctata caatctaaac agtatattac agaggatgtt gcaatggcat
    14281 tcggattttt cgatggcatg cataaaggtc atgacaaagt ctttgatata ttaaacgaaa
    14341 tagctgaggc acgcagttta aaaaaagcgg tgatgacatt tgatccgcat ccgtctgtcg
    14401 tgttgaatcc taaaagaaaa cgaacaacgt atttaacgcc actttcagat aaaatcgaaa
    14461 aaattagcca acatgatatt gattattgta tagtggttaa tttttcatct aggtttgcta
    14521 atgtgagcgt agaagatttt gttgaaaatt atataattaa aaataatgta aaagaagtca
    14581 ttgctggttt tgattttact tttggtaaat ttggaaaagg taatatgact gtacttcaag
    14641 aatatgatgc gtttaatacg acaattgtga gtaaacaaga aattgaaaat gaaaaaattt
    14701 ctacaacttc tattcgtcaa gatttaatca atggtgagtt gcaaaaagcg aatgatgctt
    14761 taggctatat atattctatt aaaggcactg tagtgcaagg tgaaaaaagg ggaagaacta
    14821 ttggcttccc aacagctaac attcaaccta gtgatgatta tttgttacct cgtaaaggtg
    14881 tttatgctgt tagtattgaa atcggcactg aaaataaatt atatcgaggg gtagctaaca
    14941 taggtgtaaa gccaacattt catgatccta acaaagcaga agttgtcatc gaagtgaata
    15001 tctttgactt tgaggataat atttatggtg aacgagtgac cgtgaattgg catcatttct
    15061 tacgtcctga gattaaattt gatggtatcg acccattagt taaacaaatg aacgatgata
    15121 aatcgcgtgc taaatattta ttagcagttg attttggtga tgaagtagct tataatatct
    15181 agagttgcgt atagttatat aaacaatcta taccacacct ttttcttagt aggtcgaatc
    15241 tccaacgcct aactcggatt aaggagtatt caaacatttt aaggaggaaa ttgattatgg
    15301 caatttcaca agaacgtaaa aacgaaatca ttaaagaata ccgtgtacac gaaactgata
    15361 ctggttcacc agaagtacaa atcgctgtac ttactgcaga aatcaacgca gtaaacgaac
    15421 acttacgtac acacaaaaaa gaccaccatt cacgtcgtgg attattaaaa atggtaggtc
    15481 gtcgtagaca tttattaaac tacttacgta gtaaagatat tcaacgttac cgtgaattaa
    15541 ttaaatcact tggtatccgt cgttaatctt aatataacgt ctttgaggtt ggggcatatt
    15601 tatgttccaa ccttaattta tattaaaaaa gctttttaca aatattaaca tttattatat
    15661 gttaagctaa tattgagtga ataataaggt tacaatgaga taaagatgat ataagtacac
    15721 ctagagtaat aatcaagata ttaaaaataa agtatgtttt tttaaaaaat ataacttata
    15781 tttatactga taagggtggg acgataagtc tattttgtaa ataatagatg gatatcccgc
    15841 tctctttttt tccaattcaa tattttataa ctaatattaa aatacgataa taaatgatat
    15901 gatataacta ttagattcaa gagaggagat ttataatgtc tcaagaaaag aaagttttta
    15961 aaactgaatg ggcaggaaga tctttaacga ttgaaacagg gcaattagct aaacaagcaa
    16021 atggcgctgt attggttcgt tatggagata cagtcgtgtt atcgacggca actgcatcaa
    16081 aagaacctcg tgatggagat ttcttcccat taacagtgaa ctatgaagaa aaaatgtacg
    16141 ctgcgggtaa aattcctggt ggatttaaaa agagagaagg acgtcctggt gacgatgcaa
    16201 cattaactgc gcgattaatt gatagaccaa ttagaccttt attccctaaa ggatataagc
    16261 atgatgttca aattatgaac atggtattaa gtgcagatcc tgattgttca ccacaaatgg
    16321 ctgcaatgat tggttcatct atggcgctta gtgtgtcgga tattccattc caagggccaa
    16381 tcgccggtgt aaatgtgggt tatattgacg gtaaatatat cattaaccca acagtagaag
    16441 aaaaagaagt ttctcgttta gaccttgaag tagctggtca taaagatgcg gtaaacatgg
    16501 tagaggcagg cgctagtgag attactgaac aagaaatgtt agaggcgatt ttctttggtc
    16561 atgaagagat tcaacgttta gttgatttcc aacaacaaat cgtcgaccac attcaacctg
    16621 ttaaacaaga atttattcca gcagagcgtg atgaagcgct agttgaacgt gtaaaatctt
    16681 taaccgaaga aaaaggactt aaagaaacag ttttaacatt tgataaacaa caacgagatg
    16741 aaaatcttga taacttaaaa gaagaaatcg tcaatgaatt tatcgatgaa gaagatccag
    16801 agaatgaatt acttattaaa gaagtttatg caattttaaa tgaattagtg aaagaagaag
    16861 ttcgacgttt aattgcagat gaaaaaatta gaccagacgg ccgtaaacct gatgaaatcc
    16921 gtccattaga ttctgaagtt ggtattttac ctagaacgca tggttcaggt ctatttacac
    16981 gtggtcagac tcaagcactt tcagttttaa cattaggtgc tttaggcgat tatcaattaa
    17041 ttgatggttt aggacctgaa gaagaaaaaa gattcatgca tcattacaac ttcccgaatt
    17101 tttcagtagg tgaaactggt ccagtacgtg cgccaggtcg tcgtgaaatt ggacatggtg
    17161 cgttaggtga aagagcatta aaatatatta ttcctgatac tgctgatttc ccatatacaa
    17221 ttcgtattgt aagtgaggta cttgaatcaa atggttcatc atctcaagcg tcaatttgtg
    17281 gatcaacatt agcattaatg gatgcgggcg taccgattaa agcaccagtt gctggtattg
    17341 ctatgggcct tgttacacgt gaagatagct atacgatttt aactgatatc caaggtatgg
    17401 aagatgcatt aggtgatatg gactttaaag tcgctggtac taaagaaggt attacagcaa
    17461 tccaaatgga tattaaaatt gacggtttaa cgcgtgaaat tatcgaagag gctctagaac
    17521 aagcgagacg tggtcgttta gaaataatga atcatatgtt acaaacaatt gatcaaccac
    17581 gtactgaatt aagtgcttac gcgccaaaag ttgtaactat gacaattaaa ccagataaga
    17641 ttagagatgt tatcggacct ggtggtaaaa aaattaacga aattattgat gaaacaggtg
    17701 ttaaattaga tattgaacaa gatggtacta tctttattgg tgctgttgat caagctatga
    17761 taaatcgtgc tcgtgaaatc attgaggaaa ttacacgtga agcggaagta ggtcaaactt
    17821 atcaagccac tgttaaacgt attgaaaaat acggtgcgtt tgtaggccta ttcccaggta
    17881 aagatgcgtt gcttcacatt tcacaaattt caaaaaatag aattgaaaaa gtggaagatg
    17941 tattaaaaat cggtgacaca attgaagtta agattactga aattgataaa caaggtcgag
    18001 taaatgcttc acatagagca ttagaagaat aatatttaaa gtcatatgac gacaatgtat
    18061 cgtcatgtga tttttttatg ccacttttta cgaagtgacc cgttttgaat ttgttgtatt
    18121 gaacatttta aaacgcttta ttattttgtg tgcaactgtt aattatcctg tatgtatagt
    18181 gattaatagt gtacatcaag tgttttttaa cttataatga atagtgagtt tatatatgga
    18241 cgggtaacaa atttaggagg taagattttg agtttaataa agaaaaagaa taaagatatt
    18301 cgcattatac cattaggcgg tgttggcgaa attgctaaaa atatgtatat cgttgaagta
    18361 gacgatgaaa tgtttatgtt agatgctgga cttatgtttc cagaagacga aatgctaggt
    18421 attgatattg ttataccaga catttcatac gtacttgaaa ataaagataa attgaagggt
    18481 atattcctta cacacggaca tgagcacgcg attggtgcag tgagttatgt tttagaacaa
    18541 ttagatgcac cagtatatgg atctaaattg acaatagcgt taattaaaga aaatatgaaa
    18601 gcccgtaata ttgataaaaa agttcgctac tatacagtta ataatgattc aattatgaga
    18661 ttcaaaaacg tgaatattag tttctttaat acgacacaca gtattcctga tagtttaggt
    18721 gtttgtattc acacttcata tggtgccatt gtgtatacag gtgaatttaa gtttgaccaa
    18781 agtttacatg gacattatgc accagatatt aaacgtatgg cagagattgg tgaagaaggc
    18841 gtatttgtct taatcagtga ttctactgag gcagagaaac ctggatataa tactccggaa
    18901 aatgtgattg aacatcatat gtatgatgct tttgcaaaag tgcgaggtcg cttgatagtt
    18961 tcatgttatg cttcgaactt tatacgtatt cagcaagttt taaatattgc tagcaagcta
    19021 aatcgtaaag tgtcattttt aggaagatca cttgaaagtt catttaatat tgctcgtaaa
    19081 atggggtatt tcgacattcc taaagatttg ctaattccta taacagaagt tgataattat
    19141 cctaaaaatg aagtgataat tatagctact ggtatgcaag gagaacctgt agaagcctta
    19201 agtcaaatgg cgcaacataa gcataaaatt atgaatatcg aagaaggcga ttctgtattt
    19261 ttagcaatta cggcttctgc taatatggaa gttatcattg cgaatacatt aaatgagctt
    19321 gtacgtgctg gcgcacatat tattccaaat aacaaaaaga ttcatgcttc aagtcatggt
    19381 tgcatggaag aattaaaaat gatgattaat attatgaaac ctgaatactt tattcctgta
    19441 caaggtgaat ttaaaatgca gatagcacat gcgaagctag cagctgaagc aggtgttgca
    19501 ccagaaaaga ttttccttgt ggaaaaagga gatgtcatta attacaacgg taaagatatg
    19561 atattaaatg aaaaggtaaa ttcaggaaat attttaatag atggcattgg tattggggat
    19621 gtaggaaata tcgtgttgag agaccgtcat cttttagcag aagatggtat ctttattgct
    19681 gttgtaacgt tagatcctaa aaatagacgt atagctgcgg gacctgaaat tcaatctcgt
    19741 gggtttgtat atgtacgtga aagtgaagac ttattacgtg aagcagaaga gaaagtacgt
    19801 gaaatagtag aggctggttt acaagaaaaa cgcatagaat ggtctgaaat taaacaaaat
    19861 atgcgtgatc aaattagtaa actattattc gaaagtacaa aacgtcgtcc tatgattatt
    19921 ccagtaattt ctgaaattta atcaaaaagt cattaacata aaagaggtca gaacaagtca
    19981 ctgaaatata atggttgtca tggacaattt acttatattt tatgatggtc aattgaaggg
    20041 gtaacgatta atctgttatc ttaagtaaat tgatacatag atgatattgt tctaacctct
    20101 ttcatcgtct gtttggacta catattctaa acatcaaata ggaaattata tataataacg
    20161 tcgttttaac taaggcaaca taaggaggtg cgtcaattgg cacaagcaaa aaagaaatcg
    20221 acagctaaga aaaaaacaac atcaaaaaaa agaacaaatt cgaggaaaaa gaagaatgat
    20281 aatccgatac gttatgtcat agctatttta gtagttgtat taatggtgtt gggtgttttc
    20341 caattaggaa taataggtcg tctaattgac agcttcttta attatttatt tgggtacagt
    20401 agatatttaa catatatttt agtactctta gcaactggtt ttattacata ctctaaacgt
    20461 attcctaaaa ctagacgaac ggctggttcg attgtattgc aaattgcatt gctatttgta
    20521 tcacagttag tttttcattt taatagtggt atcaaagctg aaagagaacc tgtactttct
    20581 tatgtgtatc agtcatacca acacagtcat ttcccaaatt ttggtggcgg tgtattaggc
    20641 ttttatttat tagagttaag cgtaccttta atttcattat ttggtgtatg tattattact
    20701 attttattat tatgctcaag tgttatttta ttaacaaacc atcaacatcg tgaagttgca
    20761 aaagttgcac tggaaaatat aaaagcttgg tttggttcat ttaatgaaaa aatgtcggaa
    20821 agaaaccaag aaaaacaatt gaagcgtgaa gaaaaagcaa gacttaaaga agaacaaaag
    20881 gcacgtcaaa atgaacagcc acaaataaaa gatgtgagtg attttacgga agtgcctcaa
    20941 gaaagagata ttccaattta tgggcatact gaaaatgaaa gtaaaagcca gagtcaacca
    21001 agtcgaaaaa aacgagtgtt tgatgcagag aatagttcga ataacatcgt aaatcatcat
    21061 caagcagatc agcaagaaca attaacagaa caaactcata acagtgttga aagtgaaaac
    21121 actattgaag aagctggtga agttacgaat gtatcgtatg ttgttccacc gttaacttta
    21181 cttaatcaac ctgcaaaaca aaaagcaaca tctaaagctg aagtgcaacg taaaggacaa
    21241 gtactagaga atacattaaa agattttggg gtaaatgcaa aagtgacaca aattaaaatt
    21301 ggtcctgcag taactcaata tgaaattcaa ccagctcaag gggttaaagt gagtaaaatt
    21361 gtaaacttgc ataatgatat tgcattagct ttagcagcaa aagatgttag aatcgaagcg
    21421 ccaatacctg gtcgttctgc agtaggtatt gaagtgccaa atgagaaaat ttcattagtt
    21481 tcactaaaag aagttttaga tgaaaaattc ccgtctaata ataaactaga agttggatta
    21541 ggaagagata tatcaggtga tccaattact gttccactaa atgaaatgcc acacttattg
    21601 gtggcaggat cgacgggtag tggtaaatct gtttgtataa atggtattat tacaagtatt
    21661 ttattaaatg ctaagccgca tgaagttaaa cttatgttaa tcgatccgaa aatggttgaa
    21721 ctaaatgttt ataacggaat tccacactta ttaattccgg ttgttacaaa tcctcataaa
    21781 gctgctcaag ctttagaaaa aattgtagct gagatggaaa gacgttatga tttattccaa
    21841 cattcatcaa ctagaaacat taaaggttat aacgaattaa tccgtaagca aaatcaagaa
    21901 ttagatgaga agcaaccaga attaccttat atcgttgtta ttgtagatga gcttgcagat
    21961 ttaatgatgg tagctggtaa agaagttgaa aatgcgattc aacgtattac acaaatggca
    22021 cgtgcagcag gtatacattt aattgtagcg acacaaagac cttctgtgga tgtaattaca
    22081 ggtatcatta aaaataatat tccatctaga atagcttttg ctgtgagttc tcaaacagat
    22141 tcaagaacta ttattggtac tggcggcgca gaaaagttac ttggtaaagg tgacatgtta
    22201 tacgttggaa atggtgactc atcacaaaca cgtattcaag gggcgttttt aagtgaccaa
    22261 gaggtgcaag atgttgtaaa ttatgtagta gaacaacaac aggcaaatta tgtaaaagaa
    22321 atggaaccag atgcaccagt ggataaatcg gaaatgaaaa gtgaagatgc tttatatgat
    22381 gaagcgtatt tgtttgttgt tgaacaacaa aaggcaagta catcattgtt acaacgccaa
    22441 tttagaattg gttataatag agcatctagg ttgatggatg atttagaacg caatcaggta
    22501 atcggtccac aaaaaggaag caagcctaga caagttttaa tagatcttaa taatgacgag
    22561 gtgtaaaaaa atgtcagaaa tgaatgcggt atataacgtt aaacaataca ttttaaattt
    22621 gattaagcaa aataaattgg aatatggtga ccaacttcca agtaatttat caattgccag
    22681 agaattaaat gtaaaaaccg acgatgttta tgaagcaatt caggcattga ttactgaaca
    22741 agtcattaaa gataattttg aagagggcac aagtgttaag tcactgcccc ctttctttta
    22801 tccattgaat gaacttataa gtattgggca aatgattaaa aatgcaggat ttgaatgcgg
    22861 aactgaatac ttaaattttg atgagcaacc agcaactatg ttagatgcaa atttgttgag
    22921 cgttgaagaa ggatatccag taaccattat agaacgatta cgaactgccg atggagaacc
    22981 ggtcgtctat tgtttagata aaattgctaa aaaagaatta acatgtacag agtatcaaat
    23041 gagcaatgga tcgattctaa gtgcgataaa agaacaaagt aatcataata tttgttatgc
    23101 tgatacagaa attgaagcgg taaattatga acctcgaata tccgaagtac tgaatgcttc
    23161 gccacacgaa ggtttgattt tattaaaaat tacgcactat aatgaatcag atgaacctat
    23221 tttgtattca ttaaattata tgaaaaatag cttagttcaa tttaaaatca ctagaaaaat
    23281 atagatgtaa gaagatatag aggaggctat atatttgagt agacaatctc aaccaaatat
    23341 acatatcaaa gtttcaccaa caactaaatt taaaacaact actatagttt ttaaatttat
    23401 ggcaccttta gaatatgaca caataacagc tagatcatta ttaagcaaat tattagttcg
    23461 agcaactaag aaatggccaa ccgataagtc gtttaataat cacttagccg atttatatgg
    23521 tgcgtatgtg aatagtacaa tttcaaaatt caaagatcag catgtcatta cattttcatt
    23581 agaaattgtt aatgaacgtt atttaagaaa cggtgaatca ttatttaatc aaggattaga
    23641 tttattacaa gaaatcattt ggaatccatt aattgaaaat aaagcattca atgataattt
    23701 tgttaaccaa gagaaaacat tattagccaa aaaaatagaa gcaatggtag ataataaagc
    23761 acaatattcg tttttaaaat tactcgacca tatgtttgaa aatgaagcat ataaatactt
    23821 atctacagga caactagaac aaatcccaca tattactgct gaaacactat atcatacata
    23881 tcaatcaatg attaataatg atcaatgttc tgtttatgtt gtcggcaatg tagaacctga
    23941 aagtgttgag aaacaaatac gtgaaaaatt tgcacttaaa ccattcgata aacatcaatt
    24001 ccaacattct actcatcatt tacacgatga agaagttgat tatattgttg aatatgatga
    24061 cgtggatcaa gctaaattaa atatgggata ccgttttcca acacaatatg gacaaagtgg
    24121 atatgctgcc tttgttgtat ttaacatgat gtttggagga gatccttcat ctgttttatt
    24181 taatgaagtg cgagaaaagc aaagtttagc gtactctata cattcacaaa ttgatggcaa
    24241 aaatggctat ttatttgttt tgagtggggt ttcaagtgat aagtacgaaa ctgcaaaaga
    24301 cactattata agtgaatttg aaaaaataaa agcaggagat ttcactgaag aaaaattaga
    24361 gttagctaaa aaagtaatca tttctcatcg atatgaatct gaagatcgtc cgaaaagtat
    24421 tatagagatt atgcataacc aaatattatt agagcaacca caaagcaaag aaacatttat
    24481 aaatgatata cagaaggtaa gtcgcgaaga tattgtttct gttgctgaaa aagcattttt
    24541 agatacaatc tatgtgttga caaaaggagg ggataaataa tgaaagagcg ttattatgaa
    24601 ttaatagacg aaagagtatt cgaacaagaa ttagaaaatg gtttacgatt atttattatc
    24661 cccaaaccag gttttcaaaa gacatttgtc acttacacta cacaatttgg ttcattagat
    24721 aatcaattca aaccccttgg acaagaccaa tttgttactg tacctgatgg agttgctcac
    24781 tttttagaac ataaattatt tgaaaaagaa gaagaagact tatttactgc gtttgctgaa
    24841 gataacgcac aagcaaatgc gtttacaagc tttgatcgta caagctactt gttcagtgca
    24901 actgataata ttgaaaacaa cattaaacgt ttacttacaa tggttgaaac gccttatttt
    24961 acaaaagaaa ctgttgataa agaaaaaggt attattgcag aagaaataaa aatgtatcaa
    25021 gagcaacctg gatataaatt aatgtttgat acattgcgtg caatgtatca acaacatcca
    25081 atacgtgttg atattgccgg tagtgtagaa agtatatacg atattacaaa agatgatttg
    25141 tatctatgtt atgaaacgtt ttatcatcca tcaaatatgg ttttatttgt tgttggcgat
    25201 gtggatcctg aagcaatatg tcgaatagta aaacaacacg aggatgctcg taataaagtt
    25261 aaccaaccca aaatcgaacg aggacttgtt gatgaaccgg aggatgttaa agaagcattt
    25321 gttactgaat ctatgaaaat tcaatcacca agactaatgc ttggttttaa aaataaacca
    25381 ttacaagaag cgcctcaaaa atatgtacaa cgtgatttag aaatgtcatt attctttgag
    25441 ttaatttttg gggaagaaac agatttttat cagaatttat taaacgaagg acttatcgat
    25501 gatacatttg gttatcaatt tgtactagag ccgacgtata gtttttcaat cgtgacaagt
    25561 gctactgaag aaccagataa attaaaaaaa ttattattag atgagttgcg tgataaaaaa
    25621 ggcaatttcc aagatgcaga agcatttgaa cttttgaaaa agcaatttat aggtgaattc
    25681 atatcaagtt taaactcacc tgaatatatt gctaatcaat atactaaatt gtattttgaa
    25741 ggtgttagcg tgtttgacat gttagacatt gttgaaaata tcacgttaga tagtattaac
    25801 gaaacgtcat cgttatattt aaatttagat cagcaagtcg atagtcgttt ggagattaaa
    25861 aagtaatgaa agcattagta ttaggtggtt ctggttcaat tggttctgag atagtcaaac
    25921 aattattaac tgatggattt gaagtttatg tgcaatatta tcgtactgat ataaatgaat
    25981 taactagcaa atttaatgat gataaagttc gttttataca agcggattta tctcaaacaa
    26041 ttgatattga caaaacattt ggtgacatta aatcattaga ctgtttaata tatgcaagtg
    26101 gtcagtcttt atatggtgtt ttacaagata tgaaagacca tgatattgat gcatgttatc
    26161 agttaaatgt cttgcaatta attcgattat gtagatattt cgttgatgtt ttacgtcaaa
    26221 gtgccaatgg aagaattatt gtaatttcat caatttgggg tgagacagga gctagtatgg
    26281 aaactattta ttcggcgatg aaaagtgcac aattaggttt cgttaaggcg cttagtcaag
    26341 agcttgcact aacatcagtg acagtaaatg ctatcgcacc tggatttgta gccggtaata
    26401 tggcaagtga gtggcaagaa gatgaacttc aagcaatgat aactgaatta ccacaacagc
    26461 gattggtttt accgagtgag gttgctcata catgcgccta tttatatcac ccaaatgcta
    26521 gaagtgttac tggaactata cagaaagtta atggtgcttg gtatatttaa aatatagaac
    26581 tatttttatg agcaaatgtt tttatgattg atgttataat atgaatatta caatgaatca
    26641 taatttgtta attaaacaag gggtgaggtt caccatgaca gttgcagaga aaaaagaatg
    26701 gtacctagaa tacgaaattg aaattaatag accgggtctt ttaggtgatg tatctagttt
    26761 attaggtatg ttaggtataa gtattgttac aattaatggt gttgatcaag gtaaacgagg
    26821 ccttttaatt aaaacagaca atcttgaaaa agttgaacgt tttgagcaaa tagctcgtgg
    26881 tataaatgaa attgaaataa caaagcttaa aaaaccagaa ttaagagacc gtcttgcagt
    26941 aagacatgga agatatattg agcaagatgc aaaagataag aaaacctttc gatttgagcg
    27001 tgaagattta ggcttgttag tagacttttt agctgaattg ttcaaagaag aaggtcataa
    27061 gttgattggc attagaggta tgccacgggt tggtaaaact gaatcaattg ttgcgggaag
    27121 tgtttgtgca cataagagat ggttatttat tagttctact ttaataaaac aaactgtacg
    27181 tagctctcta attaaagggg aatatgatgc caatcatgta tacattattg atggtgcagt
    27241 tactgccaga gaatctaatc caaaacatca agagcttgtt aacgaagtta tgacgttacc
    27301 atcaatcaaa gtcgttgaac atccagattt atttgttgaa acaagtactt gtacaatgga
    27361 agattttgac tatattatcg aattgagaga aaatgaaaat caagaaatac attacgaaga
    27421 aatgaagaaa cagacagtcc aaagtaagaa taatttagat tttggagatc cgtttggtgg
    27481 tggttttggt ttcttcgagt aagtgtaagg aggctatgaa ttgaaaacgg tcggtgaagc
    27541 gctaaaaggt agacgtgaaa ggttaggaat gactttaaca gaattagagc aacgtactgg
    27601 aattaaacgt gaaatgctag tgcatattga aaataatgaa ttcgatcaac taccgaataa
    27661 aaattacagc gaaggattta ttagaaaata tgcaagcgta gtaaatattg aacctaacca
    27721 attaattcaa gctcatcaag atgaaattcc atcgaaccaa gccgaatggg acgaagtaat
    27781 tacagttttc aataataata aagacttaga ttataagagt aaatcaaaag agccaataca
    27841 attattagta atcatgggta ttacagtttt aataacttta ttgttatgga tcatgttagt
    27901 tttaatattt taacagaaat aaattagtga gaaatgagga tgtataatga atattccgaa
    27961 ccagattacg gtttttagag tagtgttaat accagttttt atattgtttg cgttagttga
    28021 ttttggattt ggcaatgtgt catttctagg aggatatgaa ataagaattg agttattaat
    28081 cagtggtttt atttttatat tggcttccct tagcgatttt gttgatggtt atttagctag
    28141 aaaatggaat ttagttacaa atatggggaa atttttggat ccattagcgg ataaattatt
    28201 agttgcaagt gctttaattg tacttgtgca actaggacta acaaattctg tagtagcaat
    28261 cattattatt gccagagaat ttgccgtaac tggtttacgt ttactacaaa ttgaacaagg
    28321 attcgtaagt gcagctggtc aattaggtaa aattaaaaca gcagttacta tggtagcaat
    28381 tacttggttg ttattaggtg atccattggc aacattgatt ggtttgtcat taggacaaat
    28441 tttattatac attggcgtta tttttactat cttatctggt attgaatact tttataaagg
    28501 tagagatgtt tttaaacaaa aataaatatt tgtttatact agatttcatt ttcatatgga
    28561 atctagtttt tttaatccca attttagaaa ttagccacgc aattgtttat aatgatatat
    28621 tgtaaaacaa tatttgttca tttttttagg gaaaatctgt agtagcatct gatacattga
    28681 atctaaaatt gatgtgaatt tttaaatgaa atacatgaaa aaatgaatta aacgatacaa
    28741 gggggatata aatgtcaatt gccattattg ctgtaggctc agaactattg ctaggtcaaa
    28801 tcgctaatac caacggacaa tttctatcta aagtatttaa tgaaattgga caaaatgtat
    28861 tagaacataa agttattgga gataataaaa aacgtttaga atcaagtgta cgtcatgcgc
    28921 tagaaaaata tgatactgtt attttaacag gtggcttagg tcctacgaaa gatgacttaa
    28981 cgaagcatac agtggcccag attgttggta aagatttagt tattgatgag ccttctttaa
    29041 aatatattga aagctatttt gaggaacaag gacaagaaat gacacctaat aataaacaac
    29101 aggctttagt aattgaaggt tcaactgtat taacaaatca tcatggcatg gctccaggaa
    29161 tgatggtgaa ttttgaaaac aaacaaatta ttttattacc aggtccaccg aaagaaatgc
    29221 aaccaatggt gaaaaatgaa ttgttgtcac attttataaa ccataatcga attatacatt
    29281 ctgaactatt aagatttgcg ggaataggtg aatctaaagt agaaacaata ttaatagatc
    29341 ttatcgataa acagactaat cctacgattg cgcctttggc gggaagtcat gaagtatata
    29401 ttagattgac tgcaaatgcc gactcaaaag aacaagcaca atcattgatt caacctgtta
    29461 aacaagaaat tcttgatcgt attggagaat attattatgg ttcagatgac acattaattg
    29521 agcaagctgt aataaagaaa attcatgaac cttttgtaat atatgatggt attactaatg
    29581 gtgctttata tcatcgattg aaagaagtgg atttaaacga tgttctaaag ggtatgatta
    29641 atcacaatga aaactttgtt gatattaata aacctattga gcagcaatta aaagatgcag
    29701 tgcaatttgt taataaattg tttaatgtgt catcagcaat tattctatta gagtatgatg
    29761 gtgtagttca tataggctat gataataact ttgaatttaa aactgagcaa tttaaaatgt
    29821 ct
//
LOCUS       DAKTLH010000018        28939 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.018, whole genome shotgun sequence.
ACCESSION   DAKTLH010000018 DAKTLH010000000
VERSION     DAKTLH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28939
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.018"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            214..1257
                     /gene="recA"
                     /locus_tag="O0394_000715"
     CDS             214..1257
                     /gene="recA"
                     /locus_tag="O0394_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCY0498815.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
     gene            1611..3170
                     /gene="rny"
                     /locus_tag="O0394_000716"
     CDS             1611..3170
                     /gene="rny"
                     /locus_tag="O0394_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCY0498816.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
     gene            complement(3467..3682)
                     /locus_tag="O0394_000717"
     CDS             complement(3467..3682)
                     /locus_tag="O0394_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498817.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
     gene            3856..4653
                     /locus_tag="O0394_000718"
     CDS             3856..4653
                     /locus_tag="O0394_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCY0498818.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            4793..6553
                     /locus_tag="O0394_000719"
     CDS             4793..6553
                     /locus_tag="O0394_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HCY0498819.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIYGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSDGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            6554..7420
                     /locus_tag="O0394_000720"
     CDS             6554..7420
                     /locus_tag="O0394_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HCY0498820.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            7514..7807
                     /locus_tag="O0394_000721"
     CDS             7514..7807
                     /locus_tag="O0394_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HCY0498821.1"
                     /translation="MKDTLMSIQIIPKTPNNDNVIPYVDEAIKIIDESGLHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            7941..9485
                     /gene="miaB"
                     /locus_tag="O0394_000722"
     CDS             7941..9485
                     /gene="miaB"
                     /locus_tag="O0394_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCY0498822.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFIKEVE
                     PEMVIQ"
     gene            9486..9851
                     /locus_tag="O0394_000723"
     CDS             9486..9851
                     /locus_tag="O0394_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HCY0498823.1"
                     /translation="MYNKDDVLNQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            9878..10369
                     /gene="thiW"
                     /locus_tag="O0394_000724"
     CDS             9878..10369
                     /gene="thiW"
                     /locus_tag="O0394_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HCY0498824.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
     gene            10672..13290
                     /gene="mutS"
                     /locus_tag="O0394_000725"
     CDS             10672..13290
                     /gene="mutS"
                     /locus_tag="O0394_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCY0498825.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            13303..15312
                     /gene="mutL"
                     /locus_tag="O0394_000726"
     CDS             13303..15312
                     /gene="mutL"
                     /locus_tag="O0394_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCY0498826.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMEENQNDEIVIK
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDGTTVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            15318..15860
                     /locus_tag="O0394_000727"
     CDS             15318..15860
                     /locus_tag="O0394_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCY0498827.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
     gene            16325..17143
                     /locus_tag="O0394_000728"
     CDS             16325..17143
                     /locus_tag="O0394_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCY0498828.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
     gene            17272..18768
                     /gene="glpK"
                     /locus_tag="O0394_000729"
     CDS             17272..18768
                     /gene="glpK"
                     /locus_tag="O0394_000729"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCY0498829.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKA
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            18926..20599
                     /locus_tag="O0394_000730"
     CDS             18926..20599
                     /locus_tag="O0394_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HCY0498830.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            20749..21663
                     /locus_tag="O0394_000731"
     CDS             20749..21663
                     /locus_tag="O0394_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0498831.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            21681..22616
                     /gene="miaA"
                     /locus_tag="O0394_000732"
     CDS             21681..22616
                     /gene="miaA"
                     /locus_tag="O0394_000732"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCY0498832.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            22631..22864
                     /gene="hfq"
                     /locus_tag="O0394_000733"
     CDS             22631..22864
                     /gene="hfq"
                     /locus_tag="O0394_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCY0498833.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEGQASTESEE"
     gene            complement(23086..23562)
                     /locus_tag="O0394_000734"
     CDS             complement(23086..23562)
                     /locus_tag="O0394_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCY0498834.1"
                     /translation="METIYDFVVETNKGVTYKLYAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            23674..24912
                     /gene="hflX"
                     /locus_tag="O0394_000735"
     CDS             23674..24912
                     /gene="hflX"
                     /locus_tag="O0394_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCY0498835.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            24931..26169
                     /locus_tag="O0394_000736"
     CDS             24931..26169
                     /locus_tag="O0394_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0498836.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEKVITRLKNVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFETKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVNQLKEQGLI"
     gene            26412..26780
                     /locus_tag="O0394_000737"
     CDS             26412..26780
                     /locus_tag="O0394_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0498837.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            26799..28139
                     /gene="glnA"
                     /locus_tag="O0394_000738"
     CDS             26799..28139
                     /gene="glnA"
                     /locus_tag="O0394_000738"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCY0498838.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            28632..28829
                     /locus_tag="O0394_000739"
     CDS             28632..28829
                     /locus_tag="O0394_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498839.1"
                     /translation="MNCYDEIFNTIKELIENKEISSYQINKDTGISYGNINAMRRRER
                     RIENLSLKNAKILYEYAKKVL"
BASE COUNT        10714 a         3961 c         5510 g         8754 t
ORIGIN      
        1 aaattatgcg ctcataagat tattaaattg gcttagaaca acaaattaat tgtattatcg
       61 ataaaaatat aagcacgttt gttcgttttt gcgttttgat ttcaagattt tatacgaaca
      121 aatattcgca aaacacttgt attttatttt gaatccttgt atagtattgg taagataatt
      181 aaaagatagc aatttcaatt aggaggtctc gctttggata acgatcgtca aaaagcttta
      241 gatacagtaa ttaaaaatat ggagaaatct ttcggtaaag gtgccgtaat gaagttgggt
      301 gacaatatag gtcgccgagt ttcaactaca tcaactggtt cagttacatt agataatgcg
      361 ctaggtgtag gtggctatcc taaaggacga attattgaaa tttatggtcc tgaaagttct
      421 ggtaagacaa cagtagcgct tcacgctatt gctgaagtac aaagtaatgg cggggtggca
      481 gcatttatcg atgctgaaca tgctttagat ccagaatatg ctcaagcatt aggcgtagat
      541 atcgataatt tatatttatc gcaaccggat catggtgaac aaggtcttga aatcgccgaa
      601 gcatttgtta gaagtggtgc agttgatatt gtagttgtag actcagttgc tgctttaaca
      661 cctaaagctg aaattgaagg agaaatggga gacactcacg ttggtttaca agctcgttta
      721 atgtcacaag cgttacgtaa actttcaggt gctatttcta aatcaaatac aactgctatt
      781 ttcatcaacc aaattcgtga aaaagttggt gttatgttcg gtaatccaga gactacacca
      841 ggtggacgtg cattaaaatt ctatagttca gtaagactag aagtacgtcg tgcagaacag
      901 cttaaacaag gacaagaaat tgtaggtaat agaactaaaa ttaaagtcgt taaaaataaa
      961 gtggcaccac catttagagt agctgaagtt gatattatgt atggacaagg tatttctaaa
     1021 gagggtgaac ttattgattt aggtgttgaa aacgacatcg ttgataaatc aggagcatgg
     1081 tattcttaca atggcgaacg aatgggtcaa ggtaaggaaa atgttaaaat gtacttgaaa
     1141 gaaaatccac aaattaaaga agaaattgat cgtaaattga gagaaaaatt aggtatatct
     1201 gatggtgatg ttgaagaaac agaagatgca ccaaagtcat tatttgacga agaatagtac
     1261 acaaatttat atctatagtt aaacttagca aatatcctta taggattgat tgaaagtgat
     1321 attcatctca taaagctaga ataatatcta actttatggg atacactaca aatcgagact
     1381 ataaggtttt ttattttatt tattattaca ttatcaatag ttttataatc gagcttcaaa
     1441 actttagaaa atagtagaaa tagcattcaa tatagtgcaa aagtgcaaat tgataacttg
     1501 acacttatct cctataaacc gtacaattaa tttgtatgat ttatatataa tttcataaag
     1561 tcatattgaa tttcatataa agagcaaacc ctagaaaagg aggtgtttgt gtgaatttat
     1621 taagcctcct actcattttg ctggggatca ttctaggagt tgttggaggg tatgttgttg
     1681 cccgaaattt gttgcttcaa aagcaatcac aagctagaca aactgccgaa gatattgtaa
     1741 atcaagcaca taaagaagct gacaatatca aaaaagagaa attacttgag gcaaaagaag
     1801 aaaaccaaat cctaagagaa caaactgaag cagaactacg agaaagacgt agcgaacttc
     1861 aaagacaaga aacccgactt cttcaaaaag aagaaaactt agagcgcaaa tctgatctat
     1921 tagataaaaa agatgagatt ttagagcaaa aagaatcaaa aattgaagaa aaacaacaac
     1981 aagtagatgc aaaagagagt agtgttcaaa cgttaataat gaagcatgaa caagaattag
     2041 aacgcatctc cggtctcact caagaagaag ctattaatga gcaacttcaa agagtagagg
     2101 aagaactgtc acaagatatt gcagtacttg ttaaagaaaa agaaaaagaa gctaaagaaa
     2161 aagttgataa aacagcaaaa gaattattag ctacagcagt acaaagatta gcagcagatc
     2221 acacaagtga atcaacggta tcagtagtta acttacctaa tgatgagatg aaaggtcgaa
     2281 tcattggacg agaaggacga aacatccgca cacttgaaac tttaactggc attgatttaa
     2341 ttattgatga cacaccagaa gcggttatat tatctggttt tgatccaata agaagagaaa
     2401 ttgctagaac agcacttgtt aacttagtat ctgatggacg tattcatcca ggtagaattg
     2461 aagatatggt cgaaaaagct agaaaagaag tagacgatat tattagagaa gcaggtgaac
     2521 aagctacatt tgaagtgaac gcacataata tgcatcctga cttagtaaaa attgtagggc
     2581 gtttaaacta tcgtacgagt tacggtcaaa atgtacttaa acattcaatt gaagttgcgc
     2641 atcttgctag tatgttagct gctgagctag gcgaagatga gacattagcg aaacgagctg
     2701 gacttttaca tgatgttggt aaagcaattg atcatgaagt agaaggtagt catgttgaaa
     2761 tcggtgtaga attagcgaaa aaatatggtg aaaatgaaac agttattaat gcaatccatt
     2821 ctcatcatgg tgatgttgaa cctacatcta ttatatctat ccttgttgct gctgcagatg
     2881 cattgtctgc ggctcgtcca ggtgcaagaa aagaaacatt agagaattat attcgtcgat
     2941 tagaacgttt agaaacgtta tcagaaagtt atgatggtgt agaaaaagca tttgcgattc
     3001 aggcaggtag agaaatccga gtgattgtat ctcctgaaga aattgatgat ttaaaatctt
     3061 atcgattggc tagagatatt aaaaatcaga ttgaagatga attacaatat cctggtcata
     3121 tcaaggtgac agttgttcga gagactagag cagtagaata tgcgaaataa tttttgtctc
     3181 cctcacaaat tagtgaggga gcttttttaa gttgtagtct taatctagtt agacagcact
     3241 ttatcggtaa taactatatt aaacagtagt tatttgaaag taagacggac cttatattaa
     3301 ataagaagtt attgctttta ataaaaatgt tttaggcttc gtaattacta tatttatatt
     3361 atgtaaacct ataaagatga ttgcttttct atccaataaa aaagaagaga agatgtaaca
     3421 catcttctct tctgcaatat taattaggat ttatttctaa gttgagttat tttaattgta
     3481 aatctgtttt ctttaattct tttataactt ctgcagtatc ataacaattt gttgcaattg
     3541 ttgaatatct ctctgctaaa cgatatgcat taatgtaaag ctttaaactt tctttagcta
     3601 tatcctctgc atcttcgaat tttgatgggt tagacataac cactaattct gcaaattttt
     3661 ctggatcaat attaatagac atgtatttat ttacaactcc tatttatttt gatgtcttaa
     3721 tactaacata ttgaagtttt cagacaaagt aatgtctctc tataattgaa gaaaaataat
     3781 tcaaagaatt taatatttac tttaaatatt agaacgtatg aattaaacta gtatttatga
     3841 gaggatgaac aaaacatgag aataatgttt ataggggata tcgtaggtaa aattggacga
     3901 gacgcaattg aaacgtacat acctcaactg aagcaaaagt ataaaccaac agttacaatt
     3961 gtaaatgctg aaaatgcagc acatggtaaa ggtttgactg aaaaaatata taaacaatta
     4021 ctaagaaatg gtgtagattt catgactatg ggtaatcaca catatggtca acgtgaaatt
     4081 tatgatttta tagatgaagc aaaacgacta gtaagaccag cgaattttcc ggatgaagcg
     4141 ccgggaattg gtatgagatt tatacaaatt aatgatatta aacttgcagt tattaatctg
     4201 caaggaagag cgtttatgcc agatattgat gatcctttta aaaaggcaga tcaattagtc
     4261 aaggaagcac aagaacaaac tccgtttata tttgttgatt ttcatgcaga aacaacttct
     4321 gaaaagtatg caatgggatg gcatttagat ggtagagcta gcgctgttgt tggaacgcat
     4381 acacacattc aaacagcaga tgaacgtatt ttaccaaagg ggacagggta tataacggat
     4441 gttggtatga caggttttta tgatggcatt ttaggaataa ataaaacaga ggtaattgag
     4501 cgttttatca ctagtttgcc acaaagacat gttgttccaa atgaaggtag aagtgtatta
     4561 tctggtgttg ttattgattt agacaaagaa ggtaaaacaa agcacatcga acgtatattg
     4621 ataaatgatg accatccatt ttcaacattt taaaattacg taagtaaaca ttcgaattgg
     4681 accctatcgt ccattagtat gaatttaata tagtaccact gtttacatag taaatcggtg
     4741 gttctttttg ttatcattta atatgaaata tatccatagg aggcatataa ctatgaaacc
     4801 acaattatcg tggaaagttg gcggtcaaca aggcgaaggt attgaatcaa ctggggaaat
     4861 cttcgctacg gctatgaata gaaaaggata ttatttatat ggatatagac atttttcaag
     4921 tcgtatcaaa ggtggacata cgaataataa aattagagtt tctacgacgc ctgttcatgc
     4981 aattagtgat gatttagata ttttgattgc atttgaccaa gaaacaattg atgttaacca
     5041 tcatgaaatg agagaagaca gtattatttt agctgatgcc aaggctaaac ctgtgaaacc
     5101 agaaggatgt catgcacagc ttattgaatt accttttaca gcaaccgcta aagaattagg
     5161 tacagcatta atgaaaaaca tggttgcaat aggtgctact agcgcattga tgaatttgaa
     5221 tacaaataca tttgaagaac ttattactaa tatgttttct aaaaaaggtg acaaggtagt
     5281 tgaagtcaat atccaagcat taaacgaagg ttatcaatta atgcaatctc gcttacctga
     5341 aatctacggg gactttgaat tagagtcaac agatgcacta ccacatctat atatgattgg
     5401 taacgatgcc attggattag gtgcaattgc tgcaggttca caatttatgg cggcatatcc
     5461 tattacacct gcgtctgaag ttatggaata tatgattgcc aatatatcta aagtaaacgg
     5521 agcggttatt caaacagaag atgaaattgc tgctgtaact atggctattg gtgcaaatta
     5581 tggtggagtc agagcgttta cggctagtgc tggtccaggt ttatctttaa tgatggaagc
     5641 aattggatta tctggtatga ctgaaacgcc attagtcatt attaataccc aacgaggtgg
     5701 accttctact ggattaccta cgaaacaaga acagtcagat ttaatgcaaa tgatttatgg
     5761 tacacatggt gatattccaa aaattgttgt agcaccaaca gatgcagaag atgcatttta
     5821 tttaactatg gaagcattta atttagcaga acaatatcaa tgccctgtta tagttctaag
     5881 tgatttgcaa ttatctttag gtaaacaaac tgttgaaaaa ttagattata atcgtattga
     5941 aattaaacgt ggtgaaatca ttcaatctga tattgaacgt gaagaagatg ataaaggtta
     6001 tttcaagcgt tatgcgttaa catccgatgg tgtttctcct agacctatcc ccggtgttaa
     6061 aggaggtatt catcatataa ctggtgtgga acacaatgaa gaaggtaaac ctagtgaatc
     6121 tgcgtcaaat agacaacaac aaatggaaaa acgaatgcgt aaaattgagc agttactaat
     6181 tgaatcgcca gtagaagcta acttacaaca tgaggatgca gatattcttt atatcggttt
     6241 tatttctaca aaaggtgcaa ttcaagaagg tagtaaccgt ttgaatcaac aaggcataaa
     6301 agttaacact atacaaatta gacaattgca tccattccca acaagcgtta ttcaagatgc
     6361 agttaataaa gcgaagaaag tcgttgtagt ggagcacaat tatcaaggac aattggctag
     6421 tattataaaa atgaatgtca atattcatga taagattgaa aattatacaa agtatgatgg
     6481 gacacctttc ctaccacatg aaatcgaaga aaaaggcaaa ataattgcta ctgaaataaa
     6541 ggagatggta tagatggcga catttaaaga ttttagaaat aatgttaagc ctaactggtg
     6601 ccccggatgt ggcgatttct cagtacaagc tgcaattcaa aaagcagccg caaatatagg
     6661 gttagaacct gaagaagtag ctatcatcac cggtatagga tgttctggcc gtctttcagg
     6721 atatattaat tcttatggcg ttcattctat tcacggacgt gcattacctt tagctcaagg
     6781 tgtaaaaatg gcgaataaag atttaactgt tattgcatcg ggaggagatg gtgatggtta
     6841 tgctataggt atggggcata caatccatgc tttaagaaga aatatgaaca tgacgtatat
     6901 agtcatggat aatcaaattt atggtttgac aaagggacaa acatcgccgt catcagcagt
     6961 aggatttgtt actaaaacaa cgccaaaagg taatatagaa aaaaatgttg cgcctttaga
     7021 attagcatta tcatctggtg ccacatttgt agcccaaggt ttttcaagcg atattaaagg
     7081 attaacaaaa ctaattgaag atgcaattaa tcatgatgga ttttcattcg ttaatgtctt
     7141 ttcaccatgt gtgacttata ataaaattaa cacatacgat tggtttaaag aacatttaac
     7201 aagtgttgat gacattgaaa attatgattc tacagataaa caattagcga ctaaaactgt
     7261 tattgaacat gaatctttag taactggtat tgtttatcaa gataaagaaa caccatcata
     7321 tgaatctcaa attaaagagt tagatgatac accacttgct aaaagagata tcaaaattac
     7381 tgaagacacc ttcaatgcat taactgaaca atttatttaa taattaaaac tgagtcgtat
     7441 aaatgtattt ataacagatc catttatgct actcagtttt ttactattac aaaaaataaa
     7501 ggagttttta aaaatgaaag acacattaat gagtatacaa ataattccta aaacaccaaa
     7561 caatgacaat gttatacctt acgtagacga ggcgattaaa ataattgacg aatctggttt
     7621 gcattttaga gtaggtccgt tagaaacgac agtacaagga aatatgaatg aatgtttaat
     7681 tttaatacaa tcattaaatg aacgaatggt ggaacttgaa tgtccaagta ttattagcca
     7741 agttaagttt tatcatgtgc cagatggcat cactattgaa actttaactg aaaaatatga
     7801 tgaataacat taaaagtgaa gtaaactgga tttgaattgg cttgttagag atgacgtata
     7861 actttaactg tttttgcact ttatagttaa atttaatata attattaaat gatacgggca
     7921 aatagaaagg attttgtaaa gtgaacgaag aacaaagaaa agcaagttct gtagatgttt
     7981 tagctgagag agataagaaa gcagaaaaag attatagtaa atattttgaa catgtttatc
     8041 agccgcctaa tttaaaagaa gcgaaaaaaa gaggtaaaca agaagttcgt tataatagag
     8101 atttccaaat tgatgaaaaa tatcgcggta tggggaacga gcgtacattt ttaattaaaa
     8161 catatggatg tcaaatgaat gcacatgaca ctgaggtcat tgctggtata cttgaagcat
     8221 taggctatca agcaacgact gatattaaca ctgcagatgt tattttaatt aatacatgtg
     8281 cgattagaga aaatgccgag aacaaagtgt ttagtgaaat aggtaatttg aagcatttga
     8341 aaaaagaacg acctgatatt ttaatcggtg tttgtggttg tatgtcacaa gaagagtcag
     8401 tagtgaataa aattttaaaa tcgtatcaaa atgtagatat gatatttggt acacataata
     8461 ttcatcattt accagaaatt ttagaagaag catacttatc taaagcaatg gttgttgaag
     8521 tatggtctaa agaaggagac gttattgaaa atcttccaaa agtccgtgaa ggcaacatta
     8581 aagcatgggt caatattatg tatggttgtg ataagttttg tacatattgt attgttccat
     8641 ttacaagagg taaagaacga agccgtagac ctgaagacat tatagatgaa gtacgtgaac
     8701 ttgctcgtga aggttacaaa gaaataacgc ttttaggtca aaatgtaaat tcttatggta
     8761 aagatttaca ggatatagaa tatgacttag gagatctttt acaagcaatt tctaaaatag
     8821 cgattccaag agttcgtttc acaacaagtc atccttggga ctttacagat cacatgattg
     8881 atgttatttc agagggtggt aatatcgttc cgcatatcca cttgccagtt caatctggaa
     8941 ataatgcagt attaaaaata atgggtagaa aatatacacg agaaagttat ttggatttag
     9001 taaaacgaat caaagataga attcctaatg tagcattaac tacagatatt attgtagggt
     9061 atccaaatga atcagaggaa caatttgaag aaactttaac tctgtatgat gaagttggtt
     9121 ttgaacatgc atatacgtac ttgtattcac aacgtgatgg tacgcctgct gctaaaatga
     9181 aagataatgt acctttaaat gtcaaaaagg aacgattgca acgtttgaat aaaaaagttg
     9241 gtcattattc acaaatagct atgagtaagt acgaaggaca aactgtaaca gtactttgtg
     9301 aaggaagtag taaaaaagat gatcaggttc ttgctggcta cactgataaa aataagctag
     9361 ttaatttcaa agcgcctaaa gaaatgattg gtaaactagt ggaagtacga atagatgaag
     9421 ctaaacagta ttcattaaat ggcagtttta taaaggaagt agagccggaa atggtgattc
     9481 aataaatgta taataaagat gacgtgttga accaagcgga taatattgca aataaaatta
     9541 aaaatttgga tactatcaaa acatatcaac aaattgaagc acagattcat cagaaccaaa
     9601 cgataaagac taaaatggat atgttaaaaa agcatcaaaa acaagcagta aactttcaaa
     9661 attacgggaa acaaaatgcg ctagaacagt cggaacatac cattcagagt atagaagcag
     9721 aaataaatac attgcccata gttgaacagt ttcaaacttc acaatatgaa gcgaatcaat
     9781 tattgaaaat gtttgtatca acaatggaaa cacgtttaaa tgaccataat aaagccaagc
     9841 atagtgatta attacaaaca aaaaggagaa caatcatatg aaatcaagaa aactggctat
     9901 aactgcactt ttaattgcaa taaatgttgt attaagcagt attatcatca ttcctctagg
     9961 accagttaag gcagcaccag tacagcattt tgtaaatgta ttaagtgcgg tcatagtagg
    10021 tccttggtat ggattagctc aagcgcttat atcatcaatt ttaagagttc tttttggtac
    10081 tggtacagct tttgcatttc cgggtagtat gattggagtt ttattggcta gtatgtttta
    10141 catatatcgt aaacatatat ttatggccgc ggtcggtgaa gtacttggaa ctggtgtcat
    10201 cggaagttta atttgtatac cattagcata tttccttggg cttcaagact tcttcattaa
    10261 accgttaatg attacgttca tagtctcaag tgctatcgga tctattataa gttatttctt
    10321 attaattact ctaaaaaaac gtggtattct tcaaaggttt ataaaataat tttatgatat
    10381 atgatttcaa acagagtcat tgttcatttt aatattgaac gcccttactt aattaaatgt
    10441 cttgaatgtt agaattttaa tttaattcta aacatgctat tgacacattt tattaggttt
    10501 gaggcgtttt cttatacatt taagatgctt cgtgattata tggtatgaat aggtttgtaa
    10561 aagtaagtat gttaaaatgt ataagttaaa taattaataa ttaaaaagct tacaaacgaa
    10621 tatattgtaa agacaaattt taaaaagaga caagggttgt gaagaaataa tatgtctaat
    10681 gttacaccaa tgatgcagca atatttaaaa ataaaatcag aataccaaga ttgcttatta
    10741 ttttttagac taggtgattt ctatgaaatg ttttatgaag atgccaagga ggcatcacgt
    10801 gtacttgaaa ttactttaac taaaagagat gctaaaaaag aaaatccaat tccgatgtgt
    10861 ggtgttccgt atcattctgc agatagttat atagatacac ttgttaataa tggatataaa
    10921 gtagctattt gtgaacagat ggaagatccg aaacaaacga aaggtatggt tagacgtgag
    10981 gtagtaagaa ttgtgactcc aggaactgtg atggagcaag gtggtgtaga tgataaacaa
    11041 aataactata ttttaagttt tgttatgaat caacctgaaa ttgcgcttag ttactgtgat
    11101 gtttctactg gcgaattaaa ggttacacat tttaatgatg aagcgacttt attaaatgaa
    11161 attacgacga taaaccctaa cgaagttgtt atcaatgaca atatttccga taatttaaaa
    11221 agacaaatta atatggtgac agaaacaata acagtcaggg aaacgttatc atcagaaatc
    11281 tatagtgtga atcaaactga acataaatta atgtatcaag cgacacaatt attgctagat
    11341 tatattcatc atacacaaaa acgtgattta tcgcatatcg aggatgttgt tcaatatgca
    11401 gctatagatt atatgaaaat ggatttttat gctaagagaa accttgagtt aacggaaagc
    11461 attcgattaa aatcaaaaaa aggaacgcta ctttggctaa tggacgaaac gaaaacacca
    11521 atgggagcac gccgcttaaa acaatggata gatagaccac taataagtaa agaacaaatt
    11581 gaagcacgat tagatatcgt tgatgaattt agtgctcatt tcatagaaag agacacctta
    11641 agaacatatc ttaatcaagt gtatgatatt gaacgtcttg ttgggcgtgt tagttacgga
    11701 aatgttaatg cgagagattt aattcaactt aaacattcca tttctgaaat accgaatatt
    11761 aaagcattac taaattctat gaatcagaat actcttgtac aagttaatca actagaaccc
    11821 cttgatgatt tacttgatat attagaacag agtttagtag aagaaccacc aatttcagtt
    11881 aaagatggcg gactattcaa agttggtttt aatacgcaat tagatgaata tcttgaagct
    11941 tcaaaaaacg gaaaaacatg gttagcagaa ttacaagcca aagaaagaca acgtacagga
    12001 ataaaatcat tgaaaataag ctttaataaa gtgtttggtt attttataga aataacacgt
    12061 gccaacttgc aaaattttga accaagtgaa tttggttata tgaggaagca aacgttatcg
    12121 aatgctgaac gttttataac tgatgaactt aaagaaaaag aagatatcat tttaggtgcg
    12181 gaagacaaag ccatcgaatt agaatatcaa ttatttgttc agctacgtga agaagttaaa
    12241 aaatatactg aacgtttaca acaacaagct aaaattattt cagagctaga ttgtttacag
    12301 agctttgcag aaattgctca aaaatataat tacactaggc cttcatttag tgaaaataaa
    12361 acattagaat tagtggaatc taggcaccca gtagtggaaa gagtaatgga ttataatgac
    12421 tatgtgccta ataattgtcg attagataat gaaacattta tatatttaat tacaggtccg
    12481 aatatgtctg gtaaatcgac atatatgaga caagttgcca taattagtat aatggcccaa
    12541 atgggagctt atgtcccttg taaagaggca gtgttaccta tatttgatca aatattcact
    12601 agaataggtg cggcagatga tttggtttca ggtaagagta cgtttatggt agaaatgcta
    12661 gaagcacaaa aggcattaac ttatgcaaca gaggatagtt tgattatttt cgatgaaatt
    12721 ggacgtggta cttcaacgta tgacggttta gctttagcgc aggcaatgat agagtatgta
    12781 gctgaaacat cacatgctaa aacgttattt tcaacacatt atcatgaatt gacaacatta
    12841 gatcaagcat taccaagtct aaaaaatgtt cacgtcgctg ctaatgaata taaaggtgaa
    12901 cttatattct tgcataaagt caaagatggt gcagttgacg atagttatgg tattcaagtt
    12961 gcgaaattag ctgatttacc tgaaaaagtt attagcagag cacaagtgat tctaagcgag
    13021 tttgaagcgt ctgctggtaa aaaatcatcg atatcaaatt taaaaatggt cgaaaatgaa
    13081 cctgaaatta atcaagaaaa tttaaactta agtgttgaag aaacaactga tactttatct
    13141 caaaaagact ttgaacaagc atcatttgat ttgtttgaaa atgatcaaga aagcgagatt
    13201 gaactacaaa ttaaaaattt gaatttatct aatatgacac caattgaggc attggtgaag
    13261 ttaagtgaat tacaaaatca attaaaatag aggtgttgca aaatggggaa aattaaagaa
    13321 ctccaaacct cattagcaaa taaaatcgca gcaggtgaag tagttgaaag accgagttct
    13381 gttgtgaaag aactgttgga aaatgctata gatgcaggcg ctacagaaat aagcattgaa
    13441 gtagaggaat ctggcgtcca atctattcgc gtagtcgata atggaagcgg aattgaagcg
    13501 gaagacttag gattagtatt tcatagacat gcgactagta aattagatca agatgaagat
    13561 ttatttcata ttaggacatt aggattccgt ggtgaagcac tagccagtat ttcatcagtt
    13621 gctaaagtaa cattgaagac ttgcacggat aatgctaatg gaaatgaaat atatgtagaa
    13681 aatggtgaaa tattaaatca taagcctgca aaagcgaaaa aaggaacaga tatacttgta
    13741 gaatcattat tttataatac accagcacgt ttaaaatata ttaaaagttt atacactgaa
    13801 ctaggtaaaa taacagatat tgtcaacaga atggcaatga gccatccgga cattcgaata
    13861 gcactcattt cagatggcaa aacaatgtta agtacaaatg gttcaggacg aactaatgaa
    13921 gtgatggcag agatttatgg gatgaaagtt gcacgagatt tagtacatat atctggagat
    13981 acaagtgatt atcacattga aggttttgtt gcaaagcctg aacattctag aagtaataag
    14041 cactatattt ctatttttat taatggacga tacattaaaa actttatgct aaataaagcg
    14101 attttagaag gctatcatac actcttaaca ataggtaggt tcccgatttg ttatattaat
    14161 attgaaatgg atccaatctt agtagacgta aatgttcatc caacaaaact agaagtgcgt
    14221 ttatcaaaag aagagcaact ataccaattg attgtgagca aaatacaaga agcatttaaa
    14281 gaccgtatat taattcctaa aaataacttg gattatgtgc caaaaaaaaa taaagtatta
    14341 cattcattcg aacaacaaaa aatcgaattt gaacaaagac aaaacacaga gaataaccaa
    14401 gagaagacgt tttcatctga agaaagtaac agtaagccat ttatggaaga aaatcaaaac
    14461 gatgagatag ttataaaaga agattcatat aatccattcg taacgaaaac atctgaaagt
    14521 ttaatagctg atgatgaatc ttctggttat aataatacac gtgaaaaaga tgaagactac
    14581 ttcaaaaaac aacaagaaat tttacaagaa atggatcaaa catttgattc aaatgatggt
    14641 acaactgtgc aaaattatga gaataaagcg tctgatgatt attatgatgt aaacgatatt
    14701 aaaggaacaa aaagtaaaga ccctaaacga agaattccat atatggaaat tgttggacaa
    14761 gtacatggaa cgtatattat tgctcaaaat gaatttggca tgtacatgat tgaccagcat
    14821 gcagctcaag aaagaataaa atatgaatat tttcgagata aaataggtga ggttaccaat
    14881 gaagtacaag atttattaat cccgttaaca tttcattttt caaaagatga acaattagtc
    14941 attgatcaat ataaaaatga gcttcaacaa gtaggtatca tgttagaaca ttttggtggt
    15001 catgattata ttgtaagtag ctatccagtt tggttcccta aagatgaagt agaagaaatt
    15061 attaaagata tgattgagct aattttggaa gagaaaaaag tagatatcaa aaaattacgt
    15121 gaagatgtag caatcatgat gtcatgtaaa aaatccatta aagcgaatca ttatttacaa
    15181 aaacatgaaa tgtctgattt aattgatcaa ttaagagaag cggaagatcc atttacatgt
    15241 ccacatggtc gtccaataat cattaatttt tcaaaatacg aattagaaaa attatttaag
    15301 cgtgtgatgt agagaggatg aatcaagtga ataacaacat attgcctgct ataagaaaca
    15361 ttaaagattt agagaaactg attaaaacag actataaaat gtgtgtgctt ctagatatgc
    15421 atataggaca tataaaaagt attatggaat tgctgaagca aaatcatata gagtgtttta
    15481 ttcatataga tttgataaaa ggtttaagcc acgatgaatt tgcaagtgaa tttattattc
    15541 agcaatacaa gccaaaaggt atcgtatcga ctaaatctaa agtaataaaa aaagctaaat
    15601 cattaaatac tttaacgatt tttagagtat ttattattga tagtcaagca ttgaaacgca
    15661 gtatagattt gataaaaaaa gttgaacctg attttgttga agtacttcca ggtgttgcga
    15721 gtaaagcgat tcatcatatt cagaaagaaa caaacacaca agtcattgca ggtggcctaa
    15781 ttaatacaat agatgaagtc aatgaagctg ttaaaaatgg agcgaaatat gtaactacta
    15841 gttatgataa actttggtaa atcataagtc tgcaaaaatt tcatttttca cgagatttag
    15901 tcatataaca taataaaaaa gccgttttca tatgggatat gcagtaggta tcaaacaaaa
    15961 aataatcaaa taaaataatt agcacctcta aaacgattaa aatcattaaa gttttagagg
    16021 tgtttattag taaaaaatgc aactagtcat aaaaaatatg gctgaagaat gtcaattaaa
    16081 caaaagagcg tcataaataa attagttagg attggtattt ctagttttca aattaaacta
    16141 cttttataaa gacttgaaaa attaaatttg acaaaatttt tacaaaaacc attgacaacg
    16201 ctttcatatc gatagtatca taacaaatat aataaaagtt aatacataga atagagacgg
    16261 gagatttcta cgagccaaac tgctagtgta ggaatctctt tgtctttttg ggaggacatt
    16321 taatatgaat gtatatttag cagaattcct aggaactgca atcttaatcc tttttggtgg
    16381 tggcgtttgt gccaatgtca atttaaagag aagtgctgcg aatggtgctg attggattgt
    16441 catcacagct ggatggggat tagcggttac aatgggtgtg tttgctgtcg gtcaattctc
    16501 aggtgcacat ttaaacccag cggtgtcttt agctcttgca ttagacggaa gttttgattg
    16561 gtcattagtt cctggttata ttgttgctca aatgttaggt gcaattgtcg gagcaacaat
    16621 tgtatggtta atgtacttgc cacattggaa agcgacagaa gaagctggcg cgaaattagg
    16681 tgttttctct acagcaccgg ctattaagaa ttactttgcc aactttttaa gtgagattat
    16741 cggaacaatg gcattaactt taggtatttt atttatcggt gtaaacaaaa ttgccgatgg
    16801 tttaaatcct ttaattgtcg gagcattaat tgttgcaatc ggattaagtt taggcggtgc
    16861 tactggttat gcaatcaacc cagcacgtga tttaggtccg agaattgcac atgcgatttt
    16921 accaatagct ggtaaaggtg gttcaaattg gtcatatgca atcgttccta tcttaggacc
    16981 aattgccggt ggtttattag gtgcagtggt atacgctgta ttttataaac atacatttaa
    17041 tattggttgt gcaattgcaa ttgttgtagt tattattact ttgattttag gttacatttt
    17101 aaataaatca tcaaaaaaag gtgatatcga atcaatttac taaaataaaa agaaacgtaa
    17161 atagcataat ttaacatgtt tgattcatgg attatgctat tttttcgcca aaatttaaca
    17221 gattttgtac aatgggttag cgattatttt ttaataaagg agatactact aatggaaaaa
    17281 tatattttat ctatagacca aggaacaaca agctcaagag cgattttatt caatcaaaaa
    17341 ggggaaattg caggggtagc acaacgtgag tttaagcaat attttccaca atcaggttgg
    17401 gttgaacatg atgcaaatga aatttggaca tctgtgttag ctgtaatgac ggaagtaatt
    17461 aatgaaaatg atgttagagc tgatcaaatt gcaggtatcg gtattacaaa ccaacgtgaa
    17521 acaacggttg tttgggacaa acatactggc cgcccaattt atcacgcaat tgtttggcaa
    17581 tcacgtcaaa cacaatcaat ttgttcagaa ttaaaacaac aaggatatga acaaacattt
    17641 agagataaga caggattact tttagatccg tattttgcag gtacaaaagt taaatggatt
    17701 ctagacaatg ttgaaggtgc acgagaaaaa gcagaaaatg gcgatctatt atttggaacg
    17761 attgatactt ggttagtatg gaaattatca ggaaaagctg cgcatattac tgattattca
    17821 aatgcgagtc gtacattaat gtttaatatc catgatttag aatgggacga tgagttatta
    17881 gaactactta cagtacctaa aaatatgttg ccagaagtta aagcttcgag tgaagtatat
    17941 ggtaagacaa ttgattacca cttctatggt caagaagtac caatcgctgg agtagctggt
    18001 gatcaacaag cagcattatt tggacaagct tgcttcgaac gtggtgacgt gaaaaacaca
    18061 tatggaactg gtggcttcat gttaatgaat acaggtgaca aagcggttaa atctgaaagt
    18121 ggtttattaa caacaattgc ttatggtatt gatggaaaag taaattatgc gcttgaaggt
    18181 tccatctttg tttcgggttc agcaatccaa tggttacgtg atggattaag aatgattaat
    18241 tcagcaccac aatcagaaag ttatgcgaca cgagttgact ctactgaggg tgtttatgtt
    18301 gttccagctt ttgtaggttt aggaacacca tattgggatt ctgaagcacg tggtgcgatt
    18361 ttcggtttaa cacgtggaac tgaaaaagag cactttatcc gtgcaacttt agaatcacta
    18421 tgttaccaaa ctcgtgacgt tatggaagca atgtcaaaag actctggtat tgatgtccaa
    18481 agtttacgtg tcgatggtgg tgcagttaaa aataacttta ttatgcagtt ccaagcagac
    18541 attgttaata cttctgttga aagacctgaa attcaagaaa ctacagcttt aggtgctgca
    18601 tttttagcag gtttagcagt tggattctgg gagagtaaag atgatatcgc taaaaactgg
    18661 aaattagaag aaaaattcga tccgaaaatg gatgaaggcg aaagagaaaa attatataga
    18721 ggttggaaaa aagctgttga agcaacacaa gtttttaaaa cagaataaac ttgtagatta
    18781 gacttttgta taaacattgt gatacaatca atttaagtta atatttgaat cgagaagcga
    18841 gagatttgtt cgaacatgta caattgaagg attgtttatg tgggacaagt ctctcgtttt
    18901 tacatttttt aggaggcgtt ttggaatggc attgtctact tttaagagag aacatattaa
    18961 aaagaattta agaaatgatg aatatgattt agtaattatt ggtggcggta ttacaggtgc
    19021 aggtattgca ctagacgcga gtgaaagagg aatgaaagtt gcattagttg aaatgcaaga
    19081 ctttgcacaa ggaacaagct caagatctac aaaattagtc catggtggtt tgcgttactt
    19141 aaaacaattc caaattggag tagttgccga aactggtaaa gaacgtgcga ttgtttatga
    19201 aaatgggcct catgttacga ctccagagtg gatgctttta ccaatgcata aaggtggaac
    19261 atttggtaaa ttctcaacat caattggttt aggaatgtat gatcgtttag caggtgttaa
    19321 gaagtctgaa cgtaaaaaaa tgttatctaa aaaagaaact ttagctaaag aaccattagt
    19381 taaaaaagaa ggtctaaaag gcggcggtta ctatgttgaa tatcgtactg acgatgcgcg
    19441 tttaactatt gaagttatga agcgtgctgc tgaaaaaggc gcagaaatta tcaactatac
    19501 taaatctgaa cacttcactt atgataaaaa tcaacaagta aatggtgtta aagttataga
    19561 taaattaact aatgaaaatt atacaattaa ggctaaaaaa gtggttaatg cagcaggtcc
    19621 atgggttgat gatgttagaa gtggtgatta tgcacgcaat aataaaaaat tacgtttaac
    19681 taaaggtgta catgttgtta ttgatcaatc aaaattccca ttaggtcaag cagtatactt
    19741 tgatactgaa aaagatggaa gaatgatttt tgcaattcca cgtgaaggaa aagcgtatgt
    19801 aggtactaca gatacattct atgacaatat caaatcttca ccattaacta cacaagaaga
    19861 cagagactat ttaatcgatg cgattaatta catgttccct agtgttaatg ttacagatga
    19921 agatattgaa tcaacatggg caggaattag accattaatt tacgaagaag gcaaagaccc
    19981 ttctgaaatc tctcgtaagg atgaaatttg ggaaggtaaa tcaggtttat taactattgc
    20041 aggtggtaaa ttaacaggct atcgtcacat ggctcaagac attgttgatt tagtatctaa
    20101 acgcttgaaa aaagactacg gtttaacatt tagtccatgt aatacaaaag gtctggcaat
    20161 ttcaggtggc gatgtaggtg gtagcaagaa ctttgatgcg tttgtagagc aaaaagtaga
    20221 tgtagctaaa ggattcggca ttgatgaaga tgttgcaaga cgtttagcat ctaaatatgg
    20281 ttcaaatgtt gatgaattgt tcaacattgc gcaaacatct caataccatg atagcaagtt
    20341 accattagaa atttatgtag aacttgttta tagtattcaa caagaaatgg tatacaaacc
    20401 taacgatttc ttagttcgtc gttctggtaa aatgtatttc aatattaaag atgtattaga
    20461 ttataaagat gctgtcatcg atattatggc agatatgctt gattactctc cagctcaaat
    20521 tgaagcatat actgaagaag ttgagcaagc aattaaagaa gcgcaacatg gaaataatca
    20581 accagcagtt aaagaataat taatttgtac aatcataaac tggtgtcctg ttttaagggc
    20641 atcagttttt ttatacgaga tacattagtc aattgaaact atgcatcact aaatgtatga
    20701 taataataaa agcgaatata aaagcgttgt tattaaaggt gtgagatgat gactgaaaaa
    20761 caatttaaat taactgtaca agataatacg aatattgaag ttaaagtgaa ttttacagat
    20821 gtagattcaa aaggaattat tcatatattt catggtatgg ctgaacatat ggaacgttac
    20881 gataaattag cacatgcact ttcaaagcat ggcttcgatg tgatacgtca taatcatcga
    20941 ggacatggta ttaatattga tgaatcaaca agagggcatt acgatgatat gaaacgagtt
    21001 atcggtgatg cctttgaagt agcgcaaaca gtgagaggca atgttgataa accatacatt
    21061 ataatcggac attcaatggg atccgttata gctagattgt ttgtagaaac atatccgcaa
    21121 tatgttgatg gtctaatttt aagtggtact ggtatgtatt cattatggaa aggtttacca
    21181 accgttaaag tgttacaact gattacaaaa atttatggtg ctgagaaacg agttgaatgg
    21241 gttaaccagt tagtatcaaa tagttttaat aaaaaaatac gtccattacg tacacaaagt
    21301 gattggattt ctagtaatcc aattgaagta gataacttta ttaaagatcc atatagtgga
    21361 tttaatgtgt caaatcaatt attatatcaa acagcctatt atatgctaca tacatcacaa
    21421 ttaaaaaata tgaaaatgtt aaatcatgcc atgcctatat tattagtttc aggatatgac
    21481 gatcctttag gtgattatgg taaagggatt ttaaaattgg cgaatatata tagaaaagct
    21541 ggcattaaaa atgttaaagt gaatctttat catcataaac gtcatgaagt gttatttgaa
    21601 aaagatcatg acaaaatttg ggaagacttg tttaaatggt tgaatcaatt ttataaaaaa
    21661 taaagaaagt ggaattaaat atgaataaaa ataagccttt tattgtagta attgtggggc
    21721 caactgcttc aggtaaaaca gagcttagca tcgaactcgc gaagcgtatc aatggtgaaa
    21781 tcataagcgg tgactctatg caagtctaca aacatatgaa tattggaact gcaaaagtaa
    21841 cacctgaaga aatggatggt attccacatc atttaattga tatcttgaat cctgatgata
    21901 cattttcagc atatgaattc aagcgattag cagaagattt aattactgat ataacgaata
    21961 gaggtaaagt tccaatcata gcaggtggaa caggcttata tattcaatca ttaatatata
    22021 attatgaatt agaagatgaa acagttacac ctgcacaatt atccatagtt aaacaaaagt
    22081 tatctgcatt agaacattta gataatcagc aactacacga ttatttagct caatttgatg
    22141 cggtttctgc agaaaatatt caccctaaca accgccaaag agtgttgcgc gctattgaat
    22201 attatttaaa aacaaaaaaa cttttgagta atcgcaagaa agtgcaacaa tttactgaaa
    22261 attatgatac attattatta gggattgaaa tgtcgcgtaa aacattatat tcaagaataa
    22321 ataaacgtgt tgatattatg ttggatcacg gattatttag agaagtgcaa caacttgttg
    22381 aacaaggcta tgaatcttgc caaagtatgc aagctattgg atataaagaa ttaatacctg
    22441 tgattaacgg acaaatgatt tatgaagatg ctgtcaatga tttaaagcaa cattcacgcc
    22501 aatatgcaaa acgacaaatg acatggttca agaataaaat gagtgttcat tggttagata
    22561 aagaaaatat gtcacttcaa atgatgttag atgagattac aacccagatt aagtaaaagg
    22621 agtccgacag atgattgcaa acgaaaacat ccaagacaaa gcactagaga attttaaagc
    22681 aaaccaaact gaagtaactg tattctttct aaacggtttc caaatgaaag gtgttattga
    22741 agaatacgac aagtatgtcg taagcttaaa ttctcaaggc aaacaacact tgatttacaa
    22801 acatgcgatc agcacttata cagtagaaac tgaaggtcaa gcatctactg aaagtgaaga
    22861 ataagttgta atcatatggg cacgatttaa tgactcgtgt acataacgtt tgaagtatga
    22921 taaatagata tagaaataaa gtcatatgtg acatcaatta aatgatgttc aaatgacaag
    22981 atacaataga ggaatgtttg taataaaaac gcttcatata aaggtcgagt caatatatga
    23041 tacgacttta tatgaagcgt tttatttgct atgagctagt atattttata ataatttttc
    23101 tatttctctt tcgatttgaa caggtttttt ttgaggtgca aatcgtttaa caacgttacc
    23161 ttcgcgatcc actaaaaact tagtgaaatt ccatttgatt ttctcattaa agaatccgtg
    23221 ttgtgccgca gtcaaatatc taaataaagg taattgatgt tcccctttta cgtctatttt
    23281 ttgatgcata gggaaggtaa caccatagtt taatttacag ttttgagctg cttcttcgcc
    23341 tgaaccaggt tcttggccac caaattgatt acaagggaaa cctagaatta caaacccttg
    23401 atctttgtat ttctcgtata atgattgcaa accttcaaat tgtgaagtaa agccacattc
    23461 gctagctgta ttaacaatta gcataacgtc acccttatat gcatataatt tgtaagtaac
    23521 acctttattt gtttctacta caaaatcata aattgtctcc attgtatcat cctttcgatt
    23581 tacttaaaat gtaccacaaa atcgtgtaat agtctttact aaaactctat gatagaatac
    23641 tttgagtagg attttattaa ggagatgtat aacatggctc agcaacaaat tcatgatact
    23701 aaaaataaac tagaaaaagc tgtcttagtc ggtgtacatg ctcaagatga taagcaattt
    23761 aattttgagt ctacaatgga ggaattatca tctttatcag agacttgcca acttgaagtg
    23821 ttgggtcaaa ttactcaaaa cagagatcgt gtagatcgca aatattatgt tggtaaaggt
    23881 aaaattgaag aaattcaagc atttattgag ttcaaagata ttgatgtagt catcacaaat
    23941 gatgaattaa cgactgcaca atccaaatca ctaaatgaag ctttaggtgt aaaaattatt
    24001 gatagaactc agttgattct tgaaatattt gcattaagag caagaagtaa agaaggtaaa
    24061 ttgcaagtag agctagcaca acttgattat ttattaccta gattgcaagg ccatggtaaa
    24121 agcctttctc gtttaggtgg cggtattgga actagaggcc ctggtgaaac gaagttagag
    24181 atggatcgca gacatattcg aactcgtatg aatgaaatta aacatcaatt gcggacggta
    24241 gaagaacatc gcgaaagata tcgaaataaa agaaatcaaa atcaggtgtt tcaagtagct
    24301 ttagttggtt atacaaatgc tggtaaatca tcatggttta atgttttagc aaatgaagag
    24361 acgtatgaaa aagatcaatt atttgcaacg ttagatccta aaacacgaca aattcaaata
    24421 aatgatggat ttaatttaat tatttcagat actgttggtt ttatacagaa actacctacg
    24481 acgttaattg cagcttttaa atcaacttta gaagaggcta aaggtgcaga tttattagta
    24541 catgtcgtag atagtagcca tcctgaatac cgtacgcagt atgacacagt taatgattta
    24601 atcaaacaat tagatatgag tcatatttct caaatagtta tttttaataa aaaggactta
    24661 tgtgatcatg catcaaatcg tccagcaagt gatttgccta atgtttttgt ttcttctaaa
    24721 aatgatggtg ataaattact tgttaagacg ttatttattg atgaaatcaa aaggcaatta
    24781 acttattatg atgagacaat tgcgacgaat aatgcagatc gattatattt tctaaaacaa
    24841 catacattag tgactgaact taaatatgat gaaattgaaa atgtttatcg tataaaagga
    24901 tttaaaaaat aataaaagga cgaaattcaa atgaaagata taagtaagat agtagctgac
    24961 gtcgaatcaa cgttagcacc atattttaaa gaaattgaag aaacagcata tattaatcaa
    25021 gaaaaagtat taaatgcatt tcatcatgtc aaagcaaccg aaagtgatct acaaggatca
    25081 acaggatacg ggtatgatga ctttggacgt gatcatttag aagaaatata tgcgcaggca
    25141 tttaaagcag aagatgcaat tgttcgtccg caaattattt caggtacgca tgcgattact
    25201 attgcattac aaagtttatt aaaacatggt gatgaattaa tttatataac gggtagtcca
    25261 tatgacactt tacttgaagt cattggcgta aacggaaatg gtattgaaag tttaatggag
    25321 catggcgtat cgtataaaga tattgcactt aaagaaggta agatcgatat tgaaagtgtg
    25381 ttagatgggg tttctgagcg caccaaagta atagcgattc aacgttcgaa aggctatgat
    25441 caaagacctt caattccgct agatgaaatt gaaaaggtaa ttactaggtt gaaaaacgtg
    25501 catcctaata ttttaatatt tgtggataac tgttatgggg aatttgttga aagacgtgaa
    25561 cctatagaat gtggtgccga tttaatagca ggatcattaa ttaaaaaccc tggcggtggt
    25621 ttagctaaga ttggtggata cattgctggt agaaaagatt taattgaacg atgtggttat
    25681 agattgacag cacctggtat tggtaaagaa gcgggtgcat cattaaatgc attgcttgaa
    25741 atgtatcaag gtttcttttt agcaccacac gttgtcagtc agagtcttaa aggtgcattg
    25801 tttactagtt tatttttaga aaaaatgaat atgaacacaa cgccgaagta ctacgaaaaa
    25861 cgaactgatt taattcaaac agttaaattt gaaacgaaag aacaaatgat ttcattttgt
    25921 caaagtattc aacacgcatc cccaattaat gcacatttta gtccagaacc tagttatatg
    25981 cctggttacg aagatgatgt tattatggca gctggtacgt ttattcaagg ttcatcgatt
    26041 gaattatctg cagatggacc tattcgtcct ccttatgaag catatgttca aggaggatta
    26101 acatatgaac acgttaaaat tgctgtgaca agagctgtta atcagttgaa agaacaagga
    26161 cttatataat agattaaatt tcaccctaaa tgataatgtg ttttgggtga aattttttgc
    26221 ggagatttat cagattattt ttaaaaattg aaaaaatatt ttagctgtag attgttttat
    26281 gtcgttttag aagtcgaaat cgcagttata tcaatgtttt aagctttatg ttagaaaacc
    26341 tgacatattt ttgaaatcct aaaaaaatta tgataagtta ttaacaagtt caaaagtaga
    26401 ggagaggaac aatgatatcg aatgatgcaa tcagacgaaa tatggctgtc ttctctatga
    26461 gtgtagtaag taagttaacg gatttaacgc caaggcaaat acgttactat gaaacacatg
    26521 aactcatcaa acctgaaaga acagaaggtc aaaaacgtct gttctcactc aatgatttgg
    26581 aaagattact agaaattaaa tcattattag aaaaaggatt taatatcaaa gggattaaac
    26641 aaatcattta tgactcacaa gagcatttaa caacagatga acaagagata agaaaaaaga
    26701 tgattgtaga tgccacgcaa aagcctattg gagaaacttt gccaataaat cgtggtgatt
    26761 tatcccgatt tattaaataa aatttggagg attttaaaat gccaaaacgt actttcacta
    26821 aagacgacat tcgtaaattt gcagaagagg aaaatgtaag atatttaaga ttacaattca
    26881 ctgatatttt aggaacaatt aaaaatgttg aagtgcctgt aagccaatta gaaaaagtac
    26941 ttgataacga aatgatgttt gacggttctt ctatcgaagg tttcgtacgt atcgaagaat
    27001 cagatatgta cttacatcca gatttagata cttgggtaat cttcccatgg actgctggac
    27061 aaggtaaagt tgcacgttta atttgtgatg tatataaaac agatggaaca ccatttgaag
    27121 gggatcctcg tgcaaactta aaacgtgtat taaaagaaat ggaagattta ggcttcacag
    27181 actttaacct agggcctgaa ccagaattct tcttgtttaa gttggatgaa aaaggggaac
    27241 caactttaga acttaatgat gatggtggat atttcgattt agcacctaca gatttaggtg
    27301 aaaactgtcg tcgtgatatt gttttagaat tagaggatat gggcttcgat attgaagcta
    27361 gtcaccatga agttgcccct ggtcaacatg aaattgactt taaatatgca gatgctgtta
    27421 cagcatgtga taatatccaa acatttaaat tggttgttaa aacaatcgca cgtaaacata
    27481 atttacacgc aacatttatg cctaaaccat tattcggtgt gaatggtagc ggtatgcact
    27541 ttaacgtttc attattcaaa ggtaaagaaa atgcattctt tgatccaaat actgaaatgg
    27601 gcttaacgga aactgcatat caatttacag caggtgtgct taaaaatgca cgcggattta
    27661 ctgctgtatg taacccgtta gtaaactcat ataaacgttt agtacctggt tatgaagcac
    27721 catgttatat tgcatggagt ggtaaaaacc gttcaccatt aatccgtgta ccatcttcaa
    27781 gaggattatc tactcgtatc gaagtacgtt cagtagatcc agctgcaaac ccatacatgg
    27841 cgttagctgc aatcttagaa gctggactag atggtattaa aaataaatta aaagttccag
    27901 aaccagttaa ccaaaatatt tacgaaatga accgtgaaga acgtgaagca gtaggcattc
    27961 aagacttacc ttcaacactt tatactgcat taaaagcaat gcgtgaaaat gaagttatta
    28021 aaaaagcttt aggaaatcat atctataatc aatttattaa ttcaaaatca attgaatggg
    28081 attactacag aactcaagtc tctgaatggg aaagagatca gtacatgaag caatattaat
    28141 acgctaaaca atgatgggaa atcagtaata tcaagggcta gagcattttg ctttagctct
    28201 tttttgattt ggaaagatgg gatttcactt gaccactttt gttaaaaaat ggatttggtc
    28261 aagttttggt caagtaaatg ttttatgtat ttattttttt aaagctgggg aagaccattt
    28321 tatattttag ttcacatgaa ttttaatata tttggaggtg atgagatgag gcgataaaag
    28381 aacgtcgcga aaacgttgag tattacttga aaatagccat attcatatta cttctcatcg
    28441 aattcatttg cagaacgttc taaggtaaga ccccgaaaag agggacctta ctacgcatta
    28501 ttaagggggt aatactatat gaaaagagaa acaaaacaaa aaatatcttt ttgtttatcg
    28561 atcggtattt tcattttagt tatactacta ttgatattct aaaaaattaa aaatttcgcc
    28621 tcatctaatt tatgaattgc tatgatgaaa tattcaatac aatcaaagaa ttgatagaaa
    28681 acaaagagat atcgagttat caaattaata aagatactgg gataagttac ggtaatatta
    28741 atgctatgcg ccgtagagaa agaagaatag aaaatttaag cttaaagaat gcaaagatct
    28801 tatatgaata tgcgaaaaag gtattgtaat ataagcattg agttcaatag gcatacaata
    28861 actgcccaaa ctagtgactg agtgggtgct tttatagtat taaaagtttt ctaaaagtga
    28921 aagaaatcat gtttagtga
//
LOCUS       DAKTLH010000019        28756 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.019, whole genome shotgun sequence.
ACCESSION   DAKTLH010000019 DAKTLH010000000
VERSION     DAKTLH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28756
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.019"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            265..912
                     /locus_tag="O0394_000740"
     CDS             265..912
                     /locus_tag="O0394_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCY0498840.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLFFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSTIKNQPVTSETPEKACTSETPKTPEETDNQL"
     gene            1143..2042
                     /locus_tag="O0394_000741"
     CDS             1143..2042
                     /locus_tag="O0394_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0498841.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLKLESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            2035..3267
                     /locus_tag="O0394_000742"
     CDS             2035..3267
                     /locus_tag="O0394_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0498842.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGITAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGQLALITSYIPFLAPFQLVVRAQTSGLQIYEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            3251..3436
                     /locus_tag="O0394_000743"
     CDS             3251..3436
                     /locus_tag="O0394_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498843.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            3485..3811
                     /locus_tag="O0394_000744"
     CDS             3485..3811
                     /locus_tag="O0394_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCY0498844.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            3841..4860
                     /locus_tag="O0394_000745"
     CDS             3841..4860
                     /locus_tag="O0394_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCY0498845.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(5096..5749)
                     /locus_tag="O0394_000746"
     CDS             complement(5096..5749)
                     /locus_tag="O0394_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HCY0498846.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            5923..6609
                     /locus_tag="O0394_000747"
     CDS             5923..6609
                     /locus_tag="O0394_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HCY0498847.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(6675..7934)
                     /gene="sdpC"
                     /locus_tag="O0394_000748"
     CDS             complement(6675..7934)
                     /gene="sdpC"
                     /locus_tag="O0394_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HCY0498848.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            8224..9159
                     /locus_tag="O0394_000749"
     CDS             8224..9159
                     /locus_tag="O0394_000749"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HCY0498849.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            complement(9415..9834)
                     /locus_tag="O0394_000750"
     CDS             complement(9415..9834)
                     /locus_tag="O0394_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCY0498850.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(9956..10840)
                     /locus_tag="O0394_000751"
     CDS             complement(9956..10840)
                     /locus_tag="O0394_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0498851.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            11000..12661
                     /gene="hutU"
                     /locus_tag="O0394_000752"
     CDS             11000..12661
                     /gene="hutU"
                     /locus_tag="O0394_000752"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HCY0498852.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            12661..13899
                     /locus_tag="O0394_000753"
     CDS             12661..13899
                     /locus_tag="O0394_000753"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HCY0498853.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTVSFKA"
     gene            14176..15297
                     /locus_tag="O0394_000754"
     CDS             14176..15297
                     /locus_tag="O0394_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HCY0498854.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLATGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQSAEELGHGAFKIIETHALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            15408..16289
                     /locus_tag="O0394_000755"
     CDS             15408..16289
                     /locus_tag="O0394_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0498855.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKILVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            16776..17345
                     /locus_tag="O0394_000756"
     CDS             16776..17345
                     /locus_tag="O0394_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498856.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAIV
                     SNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     regulatory      17398..17493
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            17570..18883
                     /locus_tag="O0394_000757"
     CDS             17570..18883
                     /locus_tag="O0394_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HCY0498857.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     gene            19050..19556
                     /locus_tag="O0394_000758"
     CDS             19050..19556
                     /locus_tag="O0394_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCY0498858.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(19614..20378)
                     /locus_tag="O0394_000759"
     CDS             complement(19614..20378)
                     /locus_tag="O0394_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0498859.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTKKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            20708..22312
                     /locus_tag="O0394_000760"
     CDS             20708..22312
                     /locus_tag="O0394_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0498860.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASSGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            complement(22378..22908)
                     /locus_tag="O0394_000761"
     CDS             complement(22378..22908)
                     /locus_tag="O0394_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498861.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            23176..24093
                     /locus_tag="O0394_000762"
     CDS             23176..24093
                     /locus_tag="O0394_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HCY0498862.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFVSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            24168..24803
                     /locus_tag="O0394_000763"
     CDS             24168..24803
                     /locus_tag="O0394_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0498863.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            24814..25005
                     /locus_tag="O0394_000764"
     CDS             24814..25005
                     /locus_tag="O0394_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498864.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(25101..25286)
                     /locus_tag="O0394_000765"
     CDS             complement(25101..25286)
                     /locus_tag="O0394_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498865.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(25283..25639)
                     /locus_tag="O0394_000766"
     CDS             complement(25283..25639)
                     /locus_tag="O0394_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498866.1"
                     /translation="MLKQILLRATKISVLFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTVFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            25919..27298
                     /locus_tag="O0394_000767"
     CDS             25919..27298
                     /locus_tag="O0394_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0498867.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            complement(27685..28557)
                     /locus_tag="O0394_000768"
     CDS             complement(27685..28557)
                     /locus_tag="O0394_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0498868.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
BASE COUNT         9620 a         3970 c         5545 g         9621 t
ORIGIN      
        1 tatttgcata gattgagact cttttaaacg tgaacattgc agtatgacat atttttagta
       61 tagttaacga agcgttgact ttcaaatgag tctccatata gatgtgtact tgtagcgggt
      121 tttcatttta ctagtgatga aatataaagt tcattttcca aagtgattga cgattgaaat
      181 gaagtggctt gataatgaat cttgtaaatt ataacggtaa caataattaa aaatataaat
      241 atgattaacg aaagggagat tacaatggaa caattacctc aagtaggttt tatgaaatca
      301 ttcgttttat tttggaaaaa ttatgtaaat tttaagggga gatcaagacg tagtgaatat
      361 tggtatatgg cactatggca tttgatactt atggttccag ctgtgtgtgt gctaataata
      421 aacctatttc tattctttat ttctatagcg gcggataacg aaacattaat agttatgagt
      481 atattgatga ttatccttac atctgtgtat acttttgtct atagtttggc aacgtttatt
      541 ccaaatttag cgttgtgcgt tcgaagattt cacgatatat caagaacaat gttatttcca
      601 atgattatga cagtattcag tattatattt tatatagtat tacagattat cgatagttat
      661 tatgacagtg attttacatt aatgccgatg ggtcttaata ttttgttagt acttctttat
      721 tttgtttatt taggcctaac aattgttatg atagtattcc tatgctttga cagtaagccg
      781 gcaaataaat atggcgaaag cccaaaatac ccgtctacaa ttaaaaatca accagtgaca
      841 tctgaaacac cggaaaaagc gtgtacatct gaaacaccga aaacaccgga agaaacagat
      901 aatcaattgt aatgatataa atatctcggc acaatgatgc tgggatattt ttttacaaat
      961 aaggcaataa aggcatggaa aaaacatatc tagtgctaat gatacttaac atgcaaaaat
     1021 ggcactattt aaatttttca atttcagaaa ttttaaaaat aaattcattt taaaaatatt
     1081 gcatttatag agaatggaaa tgttatatta agataaatac atattttttt aggaggcaaa
     1141 gtatgggact tgtaattgaa catgttacga aacgttttgg caagatgacg gcagtaaatg
     1201 atatttcatt aaagttagaa tcaggtaaaa tgttaggttt tctaggaaga aatggtgcag
     1261 gtaaaacaac gacatttcgt atgattttag gcttaagtga accaactgaa ggacacatta
     1321 cgtataacgg taaaaaacta gataaaacaa tgtacaatcg tattggttat ttaccggaag
     1381 aacgcggttt acatgggaaa ttgacagttg aagaagaatt gaaatatttg gcaactttaa
     1441 agggaatgtc aaaaacagaa atacaacagc aaatatcgta ttggcttgag cgttttgata
     1501 ttactgaaaa ccgcaaaaaa cgaattgata gtctatcaaa agggaatcaa caaaaaattc
     1561 agctgttagc aagtatgtta cataaaccag aattactaat attagatgag cctttcagtg
     1621 gtctagaccc agttaatgtt gagttattaa aagaagcagt taaagactta aatgattggg
     1681 gcagtacaat cgtttatagt tctcaccgta tggagcatgt tgaagaatta tgtgatgatg
     1741 tttgtatttt agataaaggt caacttgttg tttctggtga tatcaatcat gtacgtgcat
     1801 ctaatggtaa caagaaagtt gtcattgaat cagaaacaac actaccagat ttaacaaaca
     1861 ttagaggcat cattcatagt gaaaacatga agcaaggtct tcagttgacg attgagaatg
     1921 aagacgtagc caaagatatt tatcaagttg tggcacatca aggttatgtt aaacgattcc
     1981 aagttgtaga accatcatta caagatatat tcattgagaa ggttggtggc aaggatgcat
     2041 aagttttggg cgacatttac tttgacatat atcaaaaaaa ttaaagctaa atcatttgta
     2101 atttttatga ttattattgc tgccttaatg attggcctat ctaacattga taagattatt
     2161 aacatgtttg atgatggtcc agataaaatt ggtgtagcag caccgaatga acaaatctat
     2221 aaggttttca agcaacaggc gaatacattt catagtgatg ctaagtttac taaagtttct
     2281 atagaagatg ctgaaaaaga agtaaaaaaa cataaacttg ataaagctta tattatcaag
     2341 gtgaatcaaa atcgcacgtt acaagggaca atcattagtg agaagcgagt aagtcatgaa
     2401 gattcacaaa aagtacaagc actgcttaca gctattcaaa caaatatggt agccggggaa
     2461 ttaaatatca acaaagaaga cttacaaaaa ttacaagcac aaagtaaagt tgataataag
     2521 gtgatttcta atgatgaagt cgataaagta tcagaaggac aaaagatttt caactatgct
     2581 ttagcttatg gcattatctt tctaatgttc ttcattgttt taaattatgc atctcaaata
     2641 gctatggaaa ttgctagtga gaaaacatcg cgtgtcattg aaatgattat tacaagtatt
     2701 tcaccaatcc aacatatttt tgctaaaatt ttaggcgtta tagcagttgc cgtgactcag
     2761 attattttaa ttgtattgat ggcagttata tgcatttttg cctttgactt aaaagacttg
     2821 ctacaaggtt ttaacgtgga aatgaatcaa ttgtcttggc aaattattat tgtcggaatt
     2881 ataagtagta ttgttggaat tacggcgtat gtattacttg cagccatcct cggatcatta
     2941 acatctagaa tagaagattt aaatcaatct ttgatgccac tgacattact tggtatgatt
     3001 gcattttata tcgcgatatt cacgataaat aatcctgatg gacaacttgc tttaatcaca
     3061 agttatattc cgtttttagc gccatttcaa ttagttgtac gcgctcaaac ttcagggctt
     3121 caaatatatg aggtcatctt aagttcttta atctcaattg taatggtagc tgtcttgata
     3181 tggattgcaa ttaaaacata taaagattca gtacttacat ttgaacgtgg cttatttaat
     3241 tcaatgaaac gtgtatttaa aaaataaaat tgatgagagt gacacacatt tatcatataa
     3301 aatgatgatg aaagcgtgtt actcttattt ttttatattt agctatagaa taatgtctaa
     3361 aaaagtgtac aatatgaatg tgaaaacgaa ctttttaaaa tgggaatgtg aagtaagaag
     3421 aaatgtaaac aaatgatatg tagccgatag attattgata gcaaatgtgt gatggagtga
     3481 tttcatgtta tatccgattt ttatatttat attagctggc ttatgtgaaa taggtggggg
     3541 atacctgatt tggctgtggc ttagggaagg acagtcttca cttgttgggc taataggcgg
     3601 tgcgatactc atgttatatg gtgtcattgc gacatttcaa tcatttccat cattcggaag
     3661 agtatatgca gcatatggtg gcgtatttat catcatgagt ttgatttttg cgatggtcgt
     3721 agataaacaa atgccagata aatatgatgt tatcggtgca atcatttgta ttgtaggtgt
     3781 tttagtaatg ttgctaccca gcagagcttg aacacttgtg gttagaaggg agaagtaaaa
     3841 atgatagttg aagttgagca ccaaagacaa ggtgtagatt taatcaaaat tgataatgat
     3901 gaaactaaaa ttatttttac gaattatgga gcaagagtag tgtcttggaa atatcatgat
     3961 aataatattg ttcttggaaa tgttgttgaa gcggatgaat tttatcatag taatcctttt
     4021 aaatttggtg cttctatagg aagatatagc ggtagaattg ataatgctaa atttaagttg
     4081 aaaggtaaag aatatcagtt agaaaaaaac aacggtgaac atcatttaca tggtggatgt
     4141 catggtttgg acaataagtt atttgattat gaaattagaa atgaaattgc tcaaattaaa
     4201 gttatattta agacggtgtt gaaatcagct gatgatcatt tcccgggtga tatcgatgtg
     4261 acgattacac atatttatga tgcggatcac caatggtcga ttgaatacga agcagttgca
     4321 tcagaagaca cattgtttag tcctacgaat catgtgtact tcaatttgaa tcgagataat
     4381 aatgtcgttg ataatcatcg catttcaagt aatcaattag atatgtatgt attagatgag
     4441 cgaaatattg ttacaggtga tattctagat ttacatgaag tgtttgaaga taacaagata
     4501 aaattgagtg atatttttac atcacaacat gcgcaattaa gccaacaaat gacacgtttt
     4561 ggtggtctcg atcacccatt tacagttggt gaacataaaa tgtatgttga aaatcatgaa
     4621 tttatgttag aggtcgatac agatatgcct catgttgtgt tctttacttt taaccagcct
     4681 gatgagtggg atagtccatt taatatttat aaaccacatt ctggctttac attagaaaca
     4741 caatttttac caaatgatat taatttatat ggtgaaaaag caccgtcaat tttaaaagct
     4801 ggtgaagtgt tcaattcgaa aacaagtttc cgtatcgaag aaaaagtaga aaatgattaa
     4861 aagtatcggc atttatataa tatttatgtt atttggcgga attttgaata ggttatcaag
     4921 aatcgtatca aatgaagtga catcattaat atacagtttg aaataaagaa aatatataac
     4981 ataaaaaagg aatcaatagc atcagtggtt atagataaga ctattataca aacgatgtgt
     5041 aagttgattc ctttttgttt tgaaaagttc atttaacact tataatgaaa ggtaattatt
     5101 tcaatgttct gttatacatt ttttgaagtt tatcgcgtct tgttggtgac agaaatggat
     5161 tgtttagtgc atatcgtaac atatcttgat tttgtctatc attataataa aggtcattaa
     5221 gctgttgtac agataaacga tgttcgattg gtgcttcttg tattaagtaa agctgatcat
     5281 ttggagatac atagccttgt tttaataccc ggaaatagaa acctgtttta ccagatgtag
     5341 acatgcgctt cactaaatca ggaatattat attttgcttg aattttccag catggttctc
     5401 taatttcaga aacctcaatt aacgcttcac ctaaacgata ttgattacca aagtaaacat
     5461 cagattcgtc taaataatca aatgttaaat tctctccaaa catcgcatga gtaggtagtg
     5521 taggtaaatc atcttgatac agtgcataat tatgcttact aaacccacaa actgctttat
     5581 gtggtccacc atgatcttta taagcctgtt catcaccagt gaaaccagtt gaagataacc
     5641 atgttgtttg tgaaatctta gttttatcta aagcagagcg catcggtctt ttcgagctat
     5701 aaggcaaatc ttggattttt cctgtcgaaa ttgcgtgaat tgatatcata tcattctcct
     5761 taacgagtat tatttaaaat atattgtact ataaaacatt tttagaattg taaattctga
     5821 ttgatatgtt taaaaaaatg ttagaaataa gggtatgtta atttatacga gtcatgtaaa
     5881 atatgaattg aatagattac ataaatatgg gaggctacct tcatgaaaga tgtcaaagca
     5941 ctcaagttaa tgacattaaa tgatgtttta agtcaaatca atggtgacat gacgctggga
     6001 attggtacag gaagtacaat ggaattactc ttacctcaaa tggcgcaact aattaaagaa
     6061 cgtggttaca atattacagg tgtatgcact tctaataaaa ttgcattttt agcaaaagaa
     6121 ttaggtatta aaatatgtga aatcaatgat gttgatcata tcgacttagc aattgatggt
     6181 gctgatgaag tagatccatc tttaaatata attaaaggtg gcggtggtgc gctgttcaga
     6241 gagaaagtta tagatgaaat ggcatcgcgt tttgttgtgg ttgtcgatga aacgaaaata
     6301 gttcaatact taggtgagac gtttaagtta ccagtagagg tggataaatt taactggtat
     6361 cacatattac gtaaaattga atcatacgct gatataaaag tagaacgtcg tgtaaacgaa
     6421 gatgtcgcat ttataactga taatggcaat tatattttag attgtaagtt accaaaagga
     6481 attgacccat ataagttcca tgagtattta attcatctga caggtgtgtt tgagacagga
     6541 tacttcttag atatggcaga tcaagttatt gtaggcactc aagagggtgt taaaatttta
     6601 gaaaaataat atatagtata gaaaagcgct tgctagcatc atctttttaa gaggtactag
     6661 caagcgcttt gttattattt gtttttatct gaagattgtt cttcagtttt ttcgatatgt
     6721 ttttctgcat caattacaac tgatgaacgt ctgtcttctt tataagtcgc aggttctttt
     6781 aatgattgct ctatttcatc ttcatttgaa tcgtttgtta aatcgtcact ttgtggtgct
     6841 tgtttaactg attctgctga ttctactgac tccgaagtga tgtcatgatc ttcatcatga
     6901 ttagattcag tttgattacc tatgtgatgt tgaggttctg ttttttcagt accttcatga
     6961 atgcttggtt cgtcagccac tgtattttca gtatcatttt tagcaacacc aaccgttgat
     7021 gcagtagcta cacctgtttt ttcagcatct ttaatatttg aagaagattt ttcagcctca
     7081 gtatgattag tttcttcatc gtcattgaca tggtctaaat aattattagg ctcaagttct
     7141 tcaaggtttc gtcttgttgt taaatatgca atacctcgga taattaagct taaaccaatg
     7201 tatcctactg caacgattgc tgttgaaatg gcgatgactt tgattgaaaa tagatcgccg
     7261 atttcttcgc taaacaagaa aataagtccg aatgtcattg aagcatggaa tgtcgttgca
     7321 atataaattg tacgtccttt agtcgctcta attaattcac caagaatcat agagaatgag
     7381 aatgtataaa ggaagttata agcagcaaat tctgtaccat aagttgtatt tgctgagaaa
     7441 actgaataca tcaaaccaac aacaatactt gcaaagaacg tgttcatttt cgtttcaaca
     7501 atattttgta agtatgaacg gaatccgaat tctactacga acgccatcag aatatgtcca
     7561 atcagaatgt gtgtaatagg tactgataag cctgttgatt gtaataaaat aaagctatct
     7621 gcaaatgtat taaagctgta cataccaata attagaataa ttaaaggtaa aattaaagct
     7681 agcaatagac gttcaattac tttcaggctg attgagaatt ttaaacctgc aagttggacc
     7741 tttttatatt tgaaaacgag tatacaaatg attgcagcaa ttaatggtgc tagatctgta
     7801 acttcaaata taaagtgttt gacaccaatt atagactgga aatctctgag cataatggat
     7861 aacgccattg taatgacaaa gaagacgaaa atcgtcattg cccattgaaa accagaaatt
     7921 ttattgttct tcattatatg taacctccat taggtaacaa gcaaaatatc ttttagtaac
     7981 aattatacat attacaagcc gagagataaa ttgtctatgt caatattaat gaaaatgtaa
     8041 tagtcttgac gtattttatt aacataaatc agctaagtat tcgttattac atagaaaata
     8101 ggggtaaaag taatgtagat aagtattttt aagatacgga tttaactatg aattgaattc
     8161 aaaatttcct atacgttata ctttaattgt taataagcca cataaataag agggggaaat
     8221 gctgtgtaca agcaaggtga accaaattta tggactggaa ggttagatag tgaaacagac
     8281 ccgaaaaaat ttagacattt tcaaacagta acatttgaag atttgtctaa gctggaaaag
     8341 agtagtatgc catcaggggt cggtatatta ggctatgctg ttgacaaagg tgttgcttta
     8401 aacaaggggc gcattggtgc aaaagaagga ccagatgcga ttaaacaagc atttgcaggt
     8461 ttgccggatt tgaatcaatg tgaaacttta gtcgattacg gaaatgttta tcatgatcat
     8521 gaggaattaa ttgatactca aaaagaattt gctatgcttg cagcgaagtc aattgctaat
     8581 catagacaaa catttttatt aggtggtgga catgatattg cgtatgctca atatttagca
     8641 acacgtaaag tctatccaac acaatctatt ggtgtgataa atattgatgc gcattttgat
     8701 acacgtgctg aacaacaatc tacatctgga acgagcttta gacaaatttt agaagaagat
     8761 gaaaacacag attatttagt gcttggtatt gctcaaggtg gtaatacgca aagtttattt
     8821 gattatgcta aagagaaaaa gattgattat gtctttgcag atgaattatt gagtcacgta
     8881 tcaccaacaa ttaaagatat gattgaacgt tttgtacatg aacatgatgt cattatgttt
     8941 acgatttgta tggatgtcat tgatagtgcg tttgcgcctg gggtaagtgc gccggcagtg
     9001 ttaggtttgt atccacatac tgttcttgaa ttagcaaaac gtattattcc aagtgataag
     9061 gtgtcttcag ttagtattgc tgagatgaat ccaacatatg acgctgacaa tagaactgct
     9121 aagctcgttg ctaatttagt gcatcatttt ttaaaataat taaaaatatt tatatgtgac
     9181 gagatttgca ttgcagggca gaatgtgagt atttcttaat agaagttata ttaacgttga
     9241 tatatttgaa aaataattgc agtaagaaga ctcttaagtt gtagtcatct tactgcttat
     9301 ttatgtttgt tgaaatagca aaaataacaa ctgtattgtt tgaaaagttc tcttattata
     9361 taataattaa ttaaaatttt aacggagttc aagaggcctt tttataatga caccttattt
     9421 gtaaaatgtc atatgtggtt tagcctcttt ataataattt aatctgttct caagtgtgcc
     9481 agtatgtaat tctagcttat gaccatcagg gtcggtaaag taaattgatt gtctatctct
     9541 aatatctcta actcttcctt ctaaaatatt cacgttatta tcttttaacc tctgatgcca
     9601 atatttaaat tcgctgtcat ctatagtgaa agctatatgt gtatatgaaa agtgaatttc
     9661 attacgtggt atatcttttt cttcatttaa agcaatccat aggcctgcaa gctcaaaata
     9721 agcagttttt ttaccagtca atagcaattt cccaagtaaa atatctctat aaaaatgtat
     9781 tgaatcgttt aaatttctga ctgaaaagca tatatgatta atagatttta acatctgctt
     9841 cacttctttc ttagaatttt tataataaat tatttttgta tcgatttttg aaatgacgtc
     9901 attaattttt atacgatgat ctgcgcaact gcataagagg ctctaataac ttaagttaaa
     9961 gagcctctaa aagtccatat aaataatctt ggcattgttg tacgaatgta tgtacttgtt
    10021 cggagtgttt gcgtttttta tggtaattga tataaatttt tcggcctaag tttggacgaa
    10081 tttttttata ttctaaatta gacgtgtgaa atgattggta ataaaatctc ggaatgatag
    10141 cgtaacctaa gccgagatga acaaatccaa ctgctgattc gaatcgatct gtttctacaa
    10201 cgacatttgg acgaatattt cttctattaa aatagtcatc taagtgtttg cgcacttgag
    10261 aatttttgtt tggtaatatg agtggcaaat tttcaacatc tacccaattt tgatttttaa
    10321 atgtttcctt gggtgctaat aaaatgtaag attcctcata taaaggaatg gatcttatat
    10381 cttcgtgggt tattttttca tttgttatag ctaaatgaat attaaaattc agtaattgct
    10441 ctatagattg atgtttatca tgtatttcat ataaacgata ttgctgttca gggtagtcgg
    10501 aatggtgctt tcgaattaaa ttcgcaatcc attgattcgt agattcaaga gtcccaattt
    10561 ttatccttgg ttctgatgta acgcttaaat catacatttt ttccatcgtg gatcgatatt
    10621 gttgaactaa ttcgctcgca taacgataaa actgtattcc tttttcggta atcttgatgt
    10681 cttttgttga acgtgtaaat aagtcataac ctaaatctgc ttccattttt ttaatcgtag
    10741 cagttaaaga tggctggcta atatgtaaaa attgtgcagc tttagtaaaa ctattatatt
    10801 tcacgatagc caagaagtat tctaactgaa taattttcat gtgttttgct ccttttattt
    10861 atagattcaa gctatggata attagtttat ttatatttgt tggctatatt ttaacatact
    10921 ataatgaata ttgaagataa taatgtaagc gttttaaatt tagtggaatt aacttcttaa
    10981 aagaacagga gcgaatttta tgagaaaaat tcaagcaaaa aaaggtttga gtattgagtg
    11041 taaaggttgg gaacaagaag cagtacttag aatgttatac aacaatttag acccagaagt
    11101 tgctgaaaga ccagaagatt tagttgttta tggtggtatt ggaaaagcag cacgtaattg
    11161 ggaagctttt gaagcaatag agaaaacgtt acgagaatta gaatcagatg agacaatgtt
    11221 agtacaatca ggaaagccag ttgctgtatt taaaacacat gaagaagcgc cacgtgtatt
    11281 aatttcaaat tcagtattag tgcctgaatg ggcaaactgg gatcatttta atgaattaga
    11341 taaaaaaggt ttaatcatgt atggacagat gacagccggt agttggatat atattggttc
    11401 tcaaggtatt gtgcaaggta cttatgagac atttgccgag ctaggtaatc aacactttaa
    11461 tggagattta gcaggcactg ttacactgac agcaggttta ggtggtatgg gtggtgcgca
    11521 accgttagca attactatga atcatggggt agcaatttgc gtggatgttg atgaaacacg
    11581 tgttgataag cgaattgata cgaaatactg tgatgttaaa acagctgatt tagatgaagc
    11641 attaaaatta gcagaagagg cgaaagaacg tggagaagga ttatcaatcg ggttagttgg
    11701 aaatgctgta gacatccatc aagcgattct agaaaaagga tttaaaattg acattattac
    11761 tgaccaaaca agtgcacatg atccgctaaa tggatatgtg ccacaaggat actctgtaga
    11821 agaagcgaaa gtattgcgtg aaaaagatcc gaaaaaatat gttgaactgt cacaagcttc
    11881 aatggcaaag catgttgaat taatgcttga attccaaaaa cgtggcgctg tagcatttga
    11941 ttatggtaac aatattcgtc aagtagcctt caataacgga gtgaaaaatg cttttgactt
    12001 cccaggcttt gtaccagctt acattagacc attattctgt gaaggtaaag ggccattccg
    12061 ctttgctgcg ttgagtggtg atccaaaaga tatcgagcgt gcggatgaag aaatgcgcaa
    12121 acttttccca gaaaatgaaa agttattaag atggcttgat ttagctgaag aaaaaatttc
    12181 atatcaagga ctaccatcac gtattgcttg gttaggctat ggagaaagag cgaaaatggg
    12241 cttagcttta aatcgtcttg tacgtgacgg tgaaatttca gcaccaattg ttattggacg
    12301 agaccattta gatgctggtt cagttgctag tcctaaccgt gaaacagaaa gtatgaaaga
    12361 tggtagtgac gcggttggtg attgggctgt attgaatgca ctcattaaca cagctgcagg
    12421 tggttcatgg atttcattcc atcacggtgg cggtgttggc atgggatatt cacttcatgc
    12481 gggtatggtt gttgtagcag atggatcaga gcgtgctgaa agaagattgg aacgtgtatt
    12541 gacgactgac ccaggtatgg gtgttgcccg acatgttgat gctggctatg acatcgctat
    12601 tcaaacagct aaagaaaaag gtattcatat tccaatgatt gataaagcag gtgataagta
    12661 atgaatgatt taataattaa tcatatagca gaattaattt taccgagatc aacagataaa
    12721 cctttgaagg gtaaggaatt agatgaatta aatgttgtga aaaatggtac agttgtcatt
    12781 aaagatggca aaattgtata tgctggaaca catacagatg actacgatgc gactgagacg
    12841 attgatgcta gtgggaaggt agtgtcccca gcattagtag atgcacatac ccatttaaca
    12901 tttggtggat ctcgagaaca tgagatgtca ttaaaacgtc aaggtaagtc ttaccttgaa
    12961 atattagaaa tgggtggtgg catattatct accgttaatg ctactagaga aacatccgaa
    13021 gatgacttgt ttaaaaaagc agagcatgat ttgcttacta tgattaaaca cggcgtgctt
    13081 gcagttgaaa gtaagagtgg ttatggatta gatagagaaa atgaactgaa gcaattgaaa
    13141 gtgtctaatc gtttagctga gaaatatgat ttagacatga aacatacttt cctagggcct
    13201 catgctgtac ctaaagaggc aagttcaaat gaggcatttt tagaagaaat gattgcgtta
    13261 cttccggaag taaaacaata tgcagacttt gcggatattt tctgtgaaac aggtgtattt
    13321 acaatagaac aatcgcaaca ttatatgcaa aaagccaaag aagcaggttt taaagtgaaa
    13381 atacatgcgg atgaaattga tccgttaggc ggactggaat tagcaattga tgaacaagca
    13441 atatcagcag accacttagt agcttctagt gataaaggaa aagaaaagtt gcgaaatagt
    13501 gatactgtag ctgtcctctt acctgcaacg acgttctatt taggtaaaga agattatgca
    13561 gatgcaagag gcatgcttga caataacggt gcgattgcat tagcaactga ttataaccct
    13621 ggtagtagtg tcacaaacaa cttacaactt gttatggcga ttgcagcctt gaaattaaag
    13681 ctatcaccta atgaagtttg gaatgctgtt acggtcaatg ctgctaaagc aatagacatt
    13741 aacgcgggta caattaacac aggtgataag gctaatttag taatttggga tgcaccaaat
    13801 catgaatata ttccatatca ttttggtatc aatcacgcag aaaaagttat taaagacggt
    13861 aaggtcatcg tagataacac agtatctttt aaagcataaa ataattagaa agctctaagc
    13921 attggtttaa actaatgttt agagcttttt gtggtttgta aaaacaagta tattcgagaa
    13981 cacgtttttg tatagagtct caattataaa agttagaata gtttgacaac agtgttacga
    14041 tattattaaa caactgtcta tcattaacga aagcaattaa caagagtaca ttgttgtgaa
    14101 atcatcgttt aatagtcaaa gatgatcatc gtagggtaga caacatatca ttgacttaag
    14161 gaaggggaat gaccgatggg attaaaagat caagctgtta cctggagaag gtattttcat
    14221 caatttccag aactatccga taaagaattt aagactacac aaaaaattaa agatatttta
    14281 acagaacatc atattagaat attagactta ccacttgcga ctgggcttgt tgctgaagtg
    14341 ggacaaggtc tgagttgtat agctgttcgg gctgacattg atgctttacc gattcaagag
    14401 cttgttgaac aggattttaa atctgaaaat gaaggtgtta tgcatgcgtg tggtcatgat
    14461 attcacatgg ctagtatttt ggctacagct gtaaaattaa aagaaattga gggcactctt
    14521 actgggcgtg ttaaattcat ttttcaatca gcagaagaac taggacatgg cgcttttaaa
    14581 attattgaaa cacatgcatt aaaagatgta caagcagtat tgggttttca taatgaccca
    14641 tcgcgttcgg taggtacatt tgcaatcaaa acaggggcaa ttacatcagc agtagatcgt
    14701 tttgagtttc atattaaagg cgtgggtggt catgctgcaa agccagaaca atgcaacgat
    14761 ccagttattg tgttggcgca attgattaat agtattcaat ccatagttag tagaaatcta
    14821 tctgcgtttg atgaagcggt agtaacaatt ggacaaatat catgtggtaa tacttggaat
    14881 gtcattgctg atcatgcgta tgtacaaggc acagtgcgta gttttgatcc tgtggtacga
    14941 aagcttgtag aaacacgatt acaagatatt gcggacggat tggctcaagc atataatatg
    15001 aaaatcaatt taaattatac gcatttacct ggcgctgtga tgaatgatga ggcattgacg
    15061 cataaagcaa ttgcagtagc ccaacatgtt ggatataagg tggaaatgat ggaacagccg
    15121 cttacgattg gagaagattt ttcaggttat agtcaacatt ttccaagtgt atttgcgtta
    15181 attggttctc atagtgaata tgatttacat catcctcaat ataaaccgga tgaaaggatt
    15241 ctggaaaaag tacctgagta ttttgttgaa tttgtaaaga gactattgca tgaataattc
    15301 gagcttgttt ttataaagaa gaaagtaatt tttagtgtaa tcatgattaa gttcctagga
    15361 agattgggta aataataacc attaagcgaa taaggagatg taaaaaaatg gcagctcaag
    15421 atcctagaac aaaatttaaa acaacggatt atgaaaaaca agaacaagag gtaccgggtt
    15481 tacaatctga aatgacgcca gcacccgatt gtggcgaaac gtcttaccaa ggtcatcaac
    15541 gcttacaagg ctataaaata ttagtcacag gtggtgactc agcaataggg cgtgcagcag
    15601 caattgcgta tgctaaagaa ggtgcagatg tagcgattaa ctatcttccg agtgaagaac
    15661 aagatgcaca agaagtacgc caagtgattg aagaaagtgg tcaaaaggca gtgttaattc
    15721 ctggtgatat aagagatgaa cagttcaact atgaccttgt tgaacaagca tatcaacaat
    15781 taggtggctt agataatgtt actttagttg ctggacatca acagtatcat gatgatattc
    15841 atggatttac aactgaggca tttacagaaa catttgaaac gaatgtctat ccgctatttt
    15901 ggacagttca aaaagcattg gaatatttaa aaccgggtgc gtcaattaca acgacatcat
    15961 ctgtacaagg ttataatcct agtcctattc ttcatgatta tgccgcttca aaagcagcaa
    16021 ttatttcatt aactaaaagt ttttcagaag aattaggacc aaaaggaatt agagtgaact
    16081 gtgtagcgcc tggtccattc tggtcaccat tacaaatttc tggtggacaa cctcaaagta
    16141 aaatcccaac atttggtcaa aaaacacctt taggtcgtgc aggtcaacct gttgagttat
    16201 gtggaactta tgtgctatta gcgtcagaag aatcaagtta tacaaccgga caagtgttcg
    16261 gtgttagcgg tggagtgcaa atagattagg tttgtatgaa gcttttaatt gaacaacata
    16321 atattaattt agttacttca atccattaaa ttaaaatgaa ttggtgaagt gtattttaaa
    16381 tagtcggtaa aggtgtgtat ctttatcggc ttattttaat actctgtcat aaggagcgtt
    16441 tgctcagggt gtggattata agttttaagc agaataaact ttcttaatga ggatgtcagg
    16501 acaacaacga aaaagttaaa aactgttgcg ggattgggtt tagggacggt tagtggaaga
    16561 aagttaaatt cattaacaag tgaaatgcct aaggctagat attaaacgtc agaacggaca
    16621 aagaataata ggatatgttt tcaattaaat agcgaagtat ttatatgtta agatgatgta
    16681 aattacatag aaacgctacc cgtagaagta ctgatcatag aaacgctgat aaacgtaaat
    16741 ttgcaaactc gatattaaac aaaggggttg ttattatggc attaatagta tctagagcta
    16801 tattattttt gattttagca ttagcttttg gaatagcgac tgtttttcct gaacaaaaat
    16861 taccattata tatagtattc acattatttg tagcagttgc gatcgtttcg aatgtatggt
    16921 ttacacatca aaacgttaaa aagcatatca ttaagtttaa caaatacgtc gcatactttt
    16981 tatcctttgt ctcagggtta ctttacattg gatttgtaat ttatacatta actataggga
    17041 gctatgatgt tttaccaatt attatatttg caataagtat cgtattggtt caaatattag
    17101 aaaatatact gggaatcagt ataaaaaata cgcgtctatc aaacaaacag ttgaaagtca
    17161 tctccaagca acgtgttcag caatttaaag tcatatcatt aatattagta agtgtgtcta
    17221 tcgtgttcat ttttattgca ttaatgctta aatggtggct tttacttgtt attagcatta
    17281 ttgttttgat tattattaca gtagcaatga ttgtttatga aaaatattat acgactcctg
    17341 attaatttat gattgacgta ctaaggatta tcttgtaacg taaacgtaaa taagcatcac
    17401 ttatctagag aggtggaggg actggcccta tgaagcctcg gcaacatctc gaatgtgcca
    17461 attccagtaa ccgtaatggt ttgaagataa gcaggtaaag cacatgaaaa cctctttctt
    17521 catcgtttgt gagaaagagg tatttttaat tggaaagcag gtaaaaagga tggaagtaca
    17581 taaaaagagc aatgcttggg cattattccc cttgttatta tttgtggcgt tgtttttagg
    17641 cgtaggtatt atcacaggtg attttacttc aatgccatta aatgttgcaa ttacgataac
    17701 ggtaattgtg gcgttattaa tgaatcgaaa agaatcattt gctaaaaaag ttgaggtctt
    17761 cacgaaaggt gcaggccatt caaacattgt tttaatgatg ttgattttta ttttagcagg
    17821 tgcattttca aatacagctg aaaaaatggg tggcgtaaag tcgacagtta atttaggact
    17881 atcattaatc ccagaaaatt taattattgt aggattgttt gtgatttgta tgtttgtttc
    17941 tatatctatg gggacgtctg taggaactgt tgctgcaata gcacctgttg gttacggatt
    18001 tgcacaagcg acagatgtac caactgcgct agcaatggct acagttgttg gaggtgcaat
    18061 gttcggcgat aatctatcaa tgatttcaga tacaacaatt gctgcggtta gaacacaaca
    18121 tacgaaaatg aaagataaat tcagagtaaa ctttaaaatt gtattgccag gtgctatttt
    18181 aacgattatt attctgtatt tcttaacgaa tggcatttct ttaaatcatg ctaaaaacta
    18241 tgattataat ctaattaaag ttgtaccgta tatcttagta ttggtattag cattattagg
    18301 tgtaaatgtc attatcgtat tgattggcgg aacattatta gccggaatca ttggtctcat
    18361 agatggttca ttcggttgga tggggctact agatgcagtg tccaaaggta ttataagcat
    18421 ggaagatatt gctatgattg cgttgctaat tggtggttta gtcggtatta ttcaacacaa
    18481 tggtggtatt gaatggttgt tgcaatttgt tcgctctaaa gtgaaatcaa agcgtggcgc
    18541 agaattagga attgctagtt tggtaagtgt cgcagatatt gccacggcaa acaacaccat
    18601 ctctattatt atgtcaggtc ctttagctaa aaatattgca gatgaatatg atgtagattc
    18661 aaggaaatca gcaagtattc ttgatatatt tggaggctgc ttccaaggtt tattaccata
    18721 tagcccgcaa gtcatttcag cggctggtgt agctggtatt tcaccgttaa tgttattccc
    18781 atacagcatt tatcctatat tattaggtgt atgcggactt attgcgattt tatttaattt
    18841 tccaaagcta aaaaagaaca ctagtcaaga aatgaaacat taatgcttaa caatatatcg
    18901 atgtagccaa gtaaggtggc ttgttatatt aacagatcat gttgtactca ccgtataacg
    18961 ctaatctttt aaaaatgatg atcatctatg tttggaactg agcaaaaata cgttaaaata
    19021 aaagtaagag cgaaagtggg tggacaacag tggcaaaatt taatgtagag aatgaacatg
    19081 tcgaagttga aattgaaaag ctttataaat tttctccaga gttagtatac gaagcttgga
    19141 caaagaaaga tttgttgaaa caatggttta tgacatcagc cagaacgaat aaagagattg
    19201 aagcggacgt taaagaaggc ggtaaatatc gtattgtaga tcaacaacgt aatggcaaag
    19261 taaatgtgat tgaaggcatt tatgaatctt tagttatgga tgaatacgtt aagatgacta
    19321 tcggtatgcc tggactaagt gaaacgcaag atgtcattga ggtagaattt tttgaacgtg
    19381 aaacaggtgg cacccaaatg ctattttatt atcgttcatt agttgaaaaa gaaagacgat
    19441 ttactaattt agaatacaag caaaagaaaa aagaatatca tgatgccatg gtgcatggtt
    19501 tcgaattgat gtttgataag atgtaccacg ttatcgaaac atcaacacaa caataaaatg
    19561 ataaaaggaa gttatgatag gctcaaggcc attaaatcat aacttccttt tttctaattt
    19621 gaaatatcat tatgtagtaa atactcatta tattttctta caagtgtttc aaagaatata
    19681 tgataactga tgaagctaga atagtctgta ccatcagtta agtagaatgt gttactggat
    19741 acatagacgt tgtaatttgc tttttgagca agggtattat ctttcattgt agtaacagaa
    19801 ataaagtatt tttgtctaag ttgaagcaag tttgttactt ctaataattc atgtgtttca
    19861 cctgataatg aaataataaa gaataaatca ttaggttcac tcttgttcaa aatcatttta
    19921 agttcatgtt catcatgaag tacaatgaca tgattatgta acgttaacaa tatacgctgt
    19981 gcctctgaag caacgtttaa ctgtgcacgc cctgtaccat acaaatatat tgttgaagct
    20041 gtatgcattt ttttagtaag aagctggtaa tcaacgcgtt ctaaataact gaatgtacta
    20101 tcgatttctt gtttgaaact atcgatagag ttagaaggta attggtgtgt cttattactt
    20161 tcaaatttaa ggtacgattt aaaatcacta taaccgtcaa aacctaattt gcgtgtaaat
    20221 ctgtgaatgg ttgcattcga ggcatgtgtg aattgcgcta aatcttgtat tttcatattt
    20281 ttacattcat ctatatgtgt attgataaag tgagcgatat gtaaatcatt atcattaagt
    20341 ttatcaaagt ttcgattaat gtgttcatct aaaaacatat tcaacctcct tgaaaataaa
    20401 tgtgtaatag atcgttatta cattaaaatc ttgtatgtca ttatatatat ttgtgtattg
    20461 atataggttt gattatgcgt ttaaaacgaa tgtattacaa atacaacgat gtcatatata
    20521 aggtcgtgaa aaaattttca ctatatgaaa agataatctg attatacaaa atactttcaa
    20581 aatacgtcaa tgtcgttgta ggtgtgtaag cgtttactat atattatatt taaatagtaa
    20641 gacatctatt tataaatgaa tctaattaga tgtattgtta ggaaagatga tatggaggga
    20701 atagaagatg aatgcgataa agcgtttcgg tagtgcaatg attgtaccgg tattaatgtt
    20761 cgctttcttt gggatagtgc ttggctttgc gacattattt aaaaatccta caatcatggg
    20821 gagtttagca gatcaacata cgttttggtt taaattttgg tcagtaatag aatctggtgg
    20881 ctgggttatt tttacgcata tggaagttgt atttgttgta ggtctaccac tttctttagc
    20941 taaaaaagca ccaggtcatg cagcacttgc agcattgatg ggttacttaa tgtttaatac
    21001 atttataaat gccattttaa cacaatggcc acatacgttt ggcgctaatt tagaaaaagg
    21061 cgttgaaaat gtacctggtt taaaatcaat tgcaggtatt gccacattgg atacgaatat
    21121 tctaggtggt attattattt cagctattat tacttggatt cacaatagat actatagtaa
    21181 acgattacca gaaatggttg gtgtattcca aggtttaaca tttgtagtta cgatttcatt
    21241 ttttgtgatg ttaccattag cagcaattac ttgtgttatt tggccaactg ttcagcacgg
    21301 tattggttca atgcaacact ttatcattgc ttcaggctat atcggtgtat ggttgtatca
    21361 cttcttagaa cgtgtattaa taccaacagg attacatcac tttatttatg caccaattga
    21421 agttggacca gtagtagtca atcacggctt gaaagctgaa tggttacaac atttaaatga
    21481 gtttgctaag agtactaagc cattaaaaga gcaattccct tatggcttta tgcttcaagg
    21541 aaatgggaaa gtatttggat gtttaggtat tgctttagca atgtatgcta cgacacctaa
    21601 agaaaatcgt aaaaaggtgg cggcactact tattcctgcc acattaacag cagtcgttgt
    21661 aggtattact gagccattag agtttacatt cttattcatc gcaccatact tatttgtttt
    21721 acacgcagtt ttagcagcat caatggatac tttaatgtac gcatttggtg ttgtaggtaa
    21781 catgggtggt ggcttgttag actttatttc aacaaactgg ttgccattag gtaaagaaca
    21841 ttggggtact tatgttgcac aagttattat cggtctgata tttgtagcga tttacttctt
    21901 cttattcaga ttcttaattt tgaaatttga tattccatta cctggacgta agaaaacaga
    21961 agaggaagtt aaattatttt ctaagcaaga ttacaaaaat aaaaaaggtg atagcgtaga
    22021 ttctaaacgt gcttcatctg gtaatgaata cgaagacaaa gctgcatatt acctcgacgg
    22081 tttaggcggc aaagaaaata tcaaagatgt tactaactgt acaacaagat tacgcttaac
    22141 tgtttatgat gaaagtaaag ttgcagatac ggaatatttc acgcatcaac aaatggcaca
    22201 cggattagta aagagtggta agagtatcca agttgtagta ggtatgacag taccacaagt
    22261 tcgtgaagca tttgaacaaa tggttgagga tcaatcgtcc gaagataaat aatacaaaaa
    22321 aagacacatc actatacgca atcattgtaa atgatcatcg tatagatgtg tcttttatta
    22381 tgatatacga gctttgctca tagatgacga tctataattg gcagatagaa tgtcgatatt
    22441 tctagttgca atcgtatgta acaatttagc aaatatttct ggcgtacata taatatgtgc
    22501 atgagactta ttcaacgtat aagtgatatc agtgtgattt tggaacatat tcataatatc
    22561 gatgactttg tctttaatga aagcatcttg caagccatca atattgatat cgaaacgctt
    22621 tgcagattga tataaattat cagaatcaag taattcattt attttctttg gtgaaagtac
    22681 ttggaaaata ctatactgat gaaaacttaa aaatgaaatg aagtatggaa gttgttgctt
    22741 tggtaagcta atttctaata catactgatt aaactgtttg tttattgaga attgcaacgc
    22801 atcacaaaat tgtacacttt tgcataactt acttaatttt tttctagtag tacggtcatt
    22861 taaaccggcg atgttgatga tgtatgtttc gaatttatca attaacatgt gaggacctcc
    22921 cgaggaatac atggcattaa atacacgttt aatatttata aaggtgactt aattttgttc
    22981 aagttgattt taccacgctt tttttcttta ttcactaaga cttttgaatg aagtttaaaa
    23041 taattgttta tcagtgataa aatatttgca ataagaagag aatgactaaa taatcttaat
    23101 tttcagaaaa gtaattgtaa ccttactggt cttatggtaa tatttttcaa tattatcgac
    23161 gaggatgtgt taacaatgtt tcaaaaattg agtatatttg ccacgaagag ttttttggta
    23221 tggatgttag tagcagctgt tattggattt attttcccac aacatgttgc gacattaggt
    23281 aaatgggtac cttatttact tggtattgtt atgttaggta tgggattaac aattacacct
    23341 aatgatttca aaatggtctt taaagcacct agagcagtaa ttattggtgt ctgtctacaa
    23401 ttcagtatta tgcccacatt agcatttata attgcaaagt cttttcattt accacctgat
    23461 attgctgttg gcgtaatatt agtcggatgt tgtccgggtg ggacatcaag taatgtaatg
    23521 agttatttag ccaaagctaa cgtagcactt tctgtttcta ttacgacggt ctctacgttg
    23581 ctagcgccat tcgttacacc tgcgttaata tatctatttg caaatgaatg gttggaagta
    23641 tctttcgtga gtatgttgtg gtcagttgtt caagttgtat taattccaat tgctttaggt
    23701 attgttttgc aaattattaa tcgtaaaatt gctgaaaaag cttctacagc tttgccaatt
    23761 atatcagttg ttgctatttc attaatttta gcaatagttg taggtggcag taagcaccaa
    23821 atcttaacta caggattatt aatattttta gtagttattt tacataacgt attagggtat
    23881 acgattggat attggttagc tcgtctttta aaattagatc gacaagatca aaaagcagtc
    23941 agtattgaag ttggaatgca gaactctggt ttagctgtgt cattagcagc attgcatttt
    24001 aatccaattg cagcagtacc aggcgcagtg tttagtttca ttcataatat aacagggcct
    24061 attttagcaa agtattggtc aaaaaagtta taattgcact aatagaatga agtggtcatc
    24121 ggactatgtt aagctttgat aaagagaaaa aatagaggag taaatatatg tatagagcag
    24181 ttatatttga tttcgatgga acaataatag atacggaaca acatttattt aatgttatta
    24241 ataaacattt agagatgcat aatgccgatc ctataagcat tgatttttat cgttcttcta
    24301 ttggaggagc agctacagat ttgcatgacc atttaattaa agcgattggt tcggaaaata
    24361 aagataaact ttatgaagaa catcatctta ctagtacaac attaccgatg attgatacga
    24421 ttaaatcatt gatggcattt ttaaagcaac gtcacattcc tatggcaatt gccacaagta
    24481 gtgtgaaagc ggaaataatg cccaccttta aagcattagg tctagacgat tatatagagg
    24541 tagttgttgg tagagaagat gttgaacaag ttaaacctga ccctgaatta tatttatctg
    24601 cagtacaaca attaaattat atgccgacac aatgtttggc tattgaagat tctgtaaatg
    24661 gtgcaacagc cgcgattgca gctggattag atgttattgt taatacgaat aaaatgacaa
    24721 gcgcacagga cttttctaat gtagattatg tagcaaaaga tattgattac gatcaaattg
    24781 tagcgcgttt ctttacgaaa taggaggcgt atcatgatgg gttacattat attgtttttt
    24841 ctagctggtc cagtaatttt aggcgttgga aatttggtga ttggtcctat atttaacaaa
    24901 cagacaccat ttcgcgtgca agtaagatct tttgttgttg gttcaatgat ttacttaata
    24961 ctcgcaacaa ttggctattt tttactatta caaggtaaac tttaacgaga aaaccacctt
    25021 acctcattaa atggacgacc atatgtatgt gaaatggtag aacgttcatg tttatgtatg
    25081 agatagggtg gtttaaatag ttacatatat tttaataata acgtcacgat gataagtaca
    25141 attaagataa tatctatgcc taccataatt gtagctcttg ttgcattact tccttgttct
    25201 tttgctgatt tcatagcacg gtagtttggc acaaagctaa taattagtaa gattaataca
    25261 attacaccaa ttaatgctgt tgtcatgatg aacgacctcc tttatttttt tcaatcaatt
    25321 cccaaataaa cgtagcaatc acaccgatga ttaacccagt gatgacaccg atttgtaccg
    25381 ttaaaaatat ggcgccgaaa atgataccaa taattaatgc aatcggtaaa gtcgtaccga
    25441 gtttaatctt gcgctctgga gaattaataa tagtaaatac tgtaagacaa atgagtatga
    25501 aagcaagtgt tgcaataata gttcttccaa ctaaatatag gatgtcaggt ttttccatac
    25561 cgatataatt tatgatgaaa aatgctacag caaagagtac cgatattttt gtagcacgta
    25621 gcagtatttg ttttaacatt gatatactcc tttttaatat tattaaaatt atatcataat
    25681 taccaagaat agctgaagtt gtatgtgact caacggtact tgagcaactt ttttaatttt
    25741 ttagaaaaat cacaaaataa ttgtttgcaa agttgcaaaa gcctgctata gtagttctgt
    25801 aaacgattgc atggtatgca aatattaatg taccaaaatc gataatttat agtataatta
    25861 cggcaataag tttttttatg gatttattta gtatcaatca gagatggggt aagaagttat
    25921 ggagaacaat gaactacaaa ggggattgag tgcccgtcaa attcaaatga ttgcacttgg
    25981 tggtacgatt ggcgtggggc ttttcatggg tgcgacaagt acaattaaat ggacaggccc
    26041 atcagttatc cttgcatatt taattgcggg tatcttttta tttttaatca tgagagcaat
    26101 gggggaaatg atttatttaa accctacaac aggatcattt gcaacatttg caagtgatta
    26161 tatacatcct gcagcaggtt atatgacagc atggagtaat atattccaat ggattgtagt
    26221 tggtatgagt gaggtcatcg cagtaggaga atatatgaag ttttggttcc cggaattgcc
    26281 aacttggatt cctggtgtta ttgctatttt attattaatg gcagcgaatt tattctcggt
    26341 aaaagcgttt ggagaatttg aattttggtt tgctttaatt aaagttgtaa caattatttt
    26401 aatgattatt gctggttttg gtcttatttt ctttggtttt ggaaatggtg gccatgcggt
    26461 aggtatttct aatctatgga caaatggcgg atttatgcca aatggtattg ttggtttctt
    26521 ctttgcatta tcaattgtaa ttggttcata ccaaggtgtg gaactgatag gtattacggc
    26581 aggtgaaact aaagatcctc agaaaaatat cgttaaagca gtgaatggtg ttatctggag
    26641 aattttaatt ttctatttag gcgctatttt tgttattgtt tcagtgtatc cttggaatca
    26701 attaggagac attggaagtc cgtttgttgc aacatttgct aaaatcggta ttacatttgc
    26761 agctggatta attaactttg ttgtattaac tgcagcaatg tcaggatgta actcaggtat
    26821 tttcagtgcg agtcgtatga tttatacact tgctcataaa ggtgaaatgc cgaaaatatt
    26881 tactaaaatc atgagaaatg gtgtaccatt gtacactgtt gtagcagtat ctcttggtat
    26941 tttaattggt gctttattaa acgtaatttt acctttatat atcgatggag cgaagagtat
    27001 tttcgtatac gtttatagtg cgtctatttt accaggtatg attccttggt ttatgatttt
    27061 attcagtcat ttacgtttta gaaaattaca ccctgaagaa ttggaaggtc acccattcaa
    27121 aatgcctggt ggcgcagtaa ctaactattt aacaatctta ttcttaatat tagtattggt
    27181 tggtatggta tttaatgttg aaactagaat atcagtgctt attggtgtca tcttcttaac
    27241 gattgtaacg atttattatt ttattagata taataaaaat aatgtaaaag caaaatagta
    27301 tgattataat gtgcgcttat cttatgacaa acttggccct agcttattaa agttaaatag
    27361 ccaaagaatg ttatgagtag gcgcattttt ttataaatat agcttaaatc gaagttgtag
    27421 tgtgaaaaac ttataagtgg caatattatc gttagttttt actgtgggct ttaaaataaa
    27481 aaatagcatt attaaaataa caaacgcaat tgatatatgg aatgaatccg ttaatcaact
    27541 gcgtttgttt ttaatataag aaattgaatg gccttatcaa atcattggtc atatgcgtat
    27601 gttatacaag ataaaagaca tgtaagcttt gaaaatgatg cataaattat tatatgacat
    27661 gattaaagaa tttatataag actgttaatg tttaaagtta atatttgata aatgtttacg
    27721 atagttatct aaagccattt tagattgtgt tatgaaatct aatgatgcgt gataatttaa
    27781 tgcaacataa cgataatata aaatatcaat agtgaacatt tgagcaaata atgaagttgt
    27841 tgctcccata tgcatctcat tttcatcagt tttcccataa gttaaaacaa tatttgatgc
    27901 ctgtgctacg ggattatccc ttgtactagt aattgtaatt ataggtatat ggtagtcatc
    27961 aataacttta accattgatt gcatttcact ttgcgtacca ttgttagtaa taagaataac
    28021 actgtcgttc gaattgtgag ttgctaataa tgtagcaaaa atatgtgttt cttgaacaag
    28081 ttgaatatta agacctattc ttgataactt ttggtataag tcggtagcaa ctacaaaaga
    28141 tgcgccaaaa ccatatataa aaatcgtctc agaacgtttt aaacaatgac atatttgatc
    28201 aatagtttta tcatttaatt cgttatttgc atgattaagt gcgcgtgtag tacgtgtatg
    28261 gagtttagtt cttaaagatt ctgtgctttc gttattcatt aattcaacat tgtaaattga
    28321 tgatgcttta ggaacatatt tagatatatt tattttcaag tcgtgaaaac cgccatcagt
    28381 aattttcttg ctgaatcgaa tgactgatga tgtactaata tctaataagc ttgctaagtc
    28441 ttgagacctc attttaatca ccttttgagg tgaatttagt ataaatttag ctatcttttt
    28501 ttcattttta gtcatatatg gatattgact atctatttct gttagtacgt ttgacatatt
    28561 aatcactcca ctttaacgca atatatttta ttagcgttgg ctattgttgt ttaacgcgat
    28621 ggtatttaca acttgtaacg taagagaatg aaaaataagc tgaatatgtt gtttgtttaa
    28681 tgatattgaa ttgtaaaact gtaaattttt ttaagatgta tagataaaaa atagcatact
    28741 cccatgtgca aataat
//
LOCUS       DAKTLH010000020        28236 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.020, whole genome shotgun sequence.
ACCESSION   DAKTLH010000020 DAKTLH010000000
VERSION     DAKTLH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28236
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.020"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(228..1226)
                     /gene="sirC"
                     /locus_tag="O0394_000769"
     CDS             complement(228..1226)
                     /gene="sirC"
                     /locus_tag="O0394_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HCY0498869.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1223..2218)
                     /gene="sirB"
                     /locus_tag="O0394_000770"
     CDS             complement(1223..2218)
                     /gene="sirB"
                     /locus_tag="O0394_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HCY0498870.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(2234..3226)
                     /gene="sirA"
                     /locus_tag="O0394_000771"
     CDS             complement(2234..3226)
                     /gene="sirA"
                     /locus_tag="O0394_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HCY0498871.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3457..4437
                     /gene="sbnA"
                     /locus_tag="O0394_000772"
     CDS             3457..4437
                     /gene="sbnA"
                     /locus_tag="O0394_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HCY0498872.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4434..5444
                     /gene="sbnB"
                     /locus_tag="O0394_000773"
     CDS             4434..5444
                     /gene="sbnB"
                     /locus_tag="O0394_000773"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HCY0498873.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5465..7219
                     /gene="sbnC"
                     /locus_tag="O0394_000774"
     CDS             5465..7219
                     /gene="sbnC"
                     /locus_tag="O0394_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HCY0498874.1"
                     /translation="MQNHTAVNTAQAIILRDLVDALLFEDIAGIVSNSEITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRVSKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTVPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7212..8468
                     /gene="sbnD"
                     /locus_tag="O0394_000775"
     CDS             7212..8468
                     /gene="sbnD"
                     /locus_tag="O0394_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HCY0498875.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            8458..10194
                     /gene="sbnE"
                     /locus_tag="O0394_000776"
     CDS             8458..10194
                     /gene="sbnE"
                     /locus_tag="O0394_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HCY0498876.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPMSKLSQVIEQSGLAPE
                     AWLECYLNRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            10103..11953
                     /gene="sbnF"
                     /locus_tag="O0394_000777"
     CDS             10103..11953
                     /gene="sbnF"
                     /locus_tag="O0394_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HCY0498877.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDMYLKDELKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEELQWQWVKGIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            11928..12704
                     /gene="sbnG"
                     /locus_tag="O0394_000778"
     CDS             11928..12704
                     /gene="sbnG"
                     /locus_tag="O0394_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HCY0498878.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDEG"
     gene            12704..13906
                     /gene="sbnH"
                     /locus_tag="O0394_000779"
     CDS             12704..13906
                     /gene="sbnH"
                     /locus_tag="O0394_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HCY0498879.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERKILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPKIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKHRF
                     PLKHINLGGGIGVNYADLTNQFEWDNFVERFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFDIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13910..14674
                     /gene="sbnI"
                     /locus_tag="O0394_000780"
     CDS             13910..14674
                     /gene="sbnI"
                     /locus_tag="O0394_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HCY0498880.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
     gene            14870..15277
                     /locus_tag="O0394_000781"
     CDS             14870..15277
                     /locus_tag="O0394_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498881.1"
                     /translation="MSSIIGKIAIWIGIVAQIYFSVVFVRMISINIAGGSDYETIFLL
                     GLILALFTVLPTIFTAIYMESYSVIGGALFIVYAIIALCLYNFLSSILWLIGGILLIW
                     NKYSKDESTDENEKVDIESTENQFESKDKITKE"
     gene            15327..15953
                     /locus_tag="O0394_000782"
     CDS             15327..15953
                     /locus_tag="O0394_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0498882.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISKGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVAAADTTTIQKSKGNANQASHKDTCKKELDSQDMM
                     EHPEVKNPTTKNLEGFNEEIHKDEATTKVVSDNTEPPIESKDHVSKKD"
     gene            16165..16941
                     /locus_tag="O0394_000783"
     CDS             16165..16941
                     /locus_tag="O0394_000783"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HCY0498883.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            17085..17207
                     /locus_tag="O0394_000784"
     CDS             17085..17207
                     /locus_tag="O0394_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498884.1"
                     /translation="MHQLKALLVLTHPRYYKTSQKHHYLIYLNKNSQSYLILFL"
     gene            17277..18257
                     /locus_tag="O0394_000785"
     CDS             17277..18257
                     /locus_tag="O0394_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCY0498885.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNNHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            18220..18912
                     /locus_tag="O0394_000786"
     CDS             18220..18912
                     /locus_tag="O0394_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCY0498886.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDKLEEVRKSYYPI
                     KRAIDLILSIVLLFLTLPIMVIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            19122..20288
                     /locus_tag="O0394_000787"
     CDS             19122..20288
                     /locus_tag="O0394_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCY0498887.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHNDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRIVFTAHGWAFTEGVKPAKKFLYLVIEKLMSLITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENEVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            20269..21507
                     /locus_tag="O0394_000788"
     CDS             20269..21507
                     /locus_tag="O0394_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCY0498888.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMSVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            21497..22927
                     /locus_tag="O0394_000789"
     CDS             21497..22927
                     /locus_tag="O0394_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCY0498889.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVINLVLCIILIYFYGIYGAAIARLITEFF
                     LLIWRFIDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
     gene            23195..23794
                     /locus_tag="O0394_000790"
     CDS             23195..23794
                     /locus_tag="O0394_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCY0498890.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            24162..24887
                     /gene="sasD"
                     /locus_tag="O0394_000791"
     CDS             24162..24887
                     /gene="sasD"
                     /locus_tag="O0394_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HCY0498891.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(25079..25834)
                     /locus_tag="O0394_000792"
     CDS             complement(25079..25834)
                     /locus_tag="O0394_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0498892.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            26070..26777
                     /gene="deoD"
                     /locus_tag="O0394_000793"
     CDS             26070..26777
                     /gene="deoD"
                     /locus_tag="O0394_000793"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCY0498893.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            26784..28136
                     /gene="tet(38)"
                     /locus_tag="O0394_000794"
     CDS             26784..28136
                     /gene="tet(38)"
                     /locus_tag="O0394_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCY0498894.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
BASE COUNT         9480 a         4349 c         5373 g         9034 t
ORIGIN      
        1 atttaaataa gaaatagtta tcgatatttg aaaaatcgaa gaatattgat tgatattata
       61 gaatatgagt cggtgttatt tgtatataaa gtattaatat gtaattaaga aagtcgacga
      121 ttgttaacaa taacaacaac tatatgtata aaaaaacctc gcaacggtta gttaactgaa
      181 tcatttagct aacttcgttg tgaggtcatt ttgttttaat aatatcgtta taactttttc
      241 acggttaata ataagtatat gaagaatggg gcaccaaaag cagcaataaa tacacctgct
      301 ggcacttctt taggcaagaa taaggtacgc ccaattaagt ctgcaataac aattgatatg
      361 gcaccaatca ttgctgacat tagtaacttt ttagcataac ttccgcgaac gattgttttc
      421 gcgatatgtg gtgcgattaa accgacaaac ccaatgttac ctactaaact gattgccata
      481 gatacgagta tagtagaagt gattaattgg attagtttca tacgttgtac atgtaagcct
      541 aagccaatcg ctacagggtc atcaagtata gatattttca tttttggtat aacaagaaat
      601 aacaacggca caacagctaa aataaccata cccaaaatga ttgtatcttt aaacgtagca
      661 ccgtaaagac ttccgactag ccatgtataa gctttggcag cagataattg cttcgttgta
      721 atgagtaatc cttggacaag cgcaataaac aacgtttgca tcgaaatacc gatgattatg
      781 agtgttgtcg ggcgtatttg tcctttcgtt tgaaacacta atagtatcat cattgcaact
      841 gcgccaccta atactgcaaa tagtggaagt aaatgtattg ttaaatggct gaaaaatgca
      901 ataaagacaa cagcacttaa gctagcacca cctgtgatac cgataatatc aggtgaggca
      961 attggatttt ttaatacatt ttgcaacatt aaaccactca ttcctagtgc ggcacctgct
     1021 aaaatcgcaa gtgtaatgcg aggtaagcgt aatacttcta aagtgaattg atccatactg
     1081 tcatttggat ttataaagta catcagtacg cgttgtaatg gtataaagct tgaaccaatc
     1141 atcatactta ccactgaaac gatggctaaa aagattaacg cgaagatgag atggtaattg
     1201 tcttttttat taatcttttc ggtcataagc gttgacgtcc tttcttcata atatagatta
     1261 agacaatagc gccaatgaca gcggtaacga caccgatagg caactctagt ggcttaatta
     1321 ttatacgagc aacaatgtct gaaatgatca ttaggattgc tccagctaat gcagtaaaag
     1381 gaattaaata cttatagttt ggtggtaata atcgtttgct aatattcggt acgataagac
     1441 ccacaaagac gattgatcca gctacggcta ccgaaatacc ggctaacata ctgatgagca
     1501 taataatcat ccatttgatt aattttatgt tttgaccgag gccggttgca atgtcgtcac
     1561 ttgtcatcaa gatgttgatg tgtgcagcca tgctaaatgc aattaaaata agtatcaata
     1621 caagcggaat aatccatggg atatcccaaa tattacgtaa tgaaacggag ccacttaacc
     1681 aaaataatag gccttgtaag tctgtttcgt tcataataag tatgccttga gtaaaggctg
     1741 taaatagcat cgcaatcgca gcacctgcca aaatgacacg gtgaggtgag aatagtgttt
     1801 gtctaaacat acctagtgca acaactaata cagtaacaac aatagccccc aaaaatgcaa
     1861 taactacaat cattttaaaa gattgaattt ggataaatgt aatactaaaa atgacaaaaa
     1921 atactgcgcc tgcattgaca ccgaaaagcc ctggtgaggc tattgggttt cgtgtaagtg
     1981 cttgcatcaa caaacctgag acagcaaggg cagcaccagt caataacgca atgattgttc
     2041 tcgacgcccg tgcaccagtg acaacatcat gtaaatcgtt ttcactatca aagttgaata
     2101 acgcctgtat caccgtacct ggtgacacaa gcgtatttcc aatcattaaa cttaagatag
     2161 ctactattgc aagacataaa ccagcaataa cgatttggta ttttggttta agtagcatcg
     2221 taaaactcct taattatttt gattgttttt caatatttaa cttttcatat aaatcgtcaa
     2281 taagttttaa tgaagattta tatccgccag ctaagttcca agtgatttca tctaaatcat
     2341 cagatacttg gttgttttta actgcgtcta aatttttcca ctctttactt gaagtccatt
     2401 cgctttcagt ctttttaact aatgcagcat ctttcgcatt tggatctgat tttactacaa
     2461 aaatatgatc agcgttcatt aatggaatgc tttctttaga tgtaagttgg ataatatctt
     2521 taccattatc aacttgtttt tgtaagtctt tattacgttt gaatcctaaa tcatttaaga
     2581 tttcaccagc atatccacca gcataaattc ttgtatgatc agcacggaag ttaacaactg
     2641 aagctttcaa tggccatgca tctttatact ttgcttttgc atctttttgg aatgcagcta
     2701 ctttatcatc gtacttttta agtaaatctt cagcttcttt ttctttccct aaagctttcc
     2761 ccattaactt agttgtatct ttgaatttga aaactgtatc agtagaaact gttggtgcga
     2821 ttttagataa ttgatcgtaa actttttcat ttctaacttt tgacgcgaca attaagtccg
     2881 gttttaattt agagatttcc tctaagttag gtgcaggttc ttgacctaca atcttagtat
     2941 cttttaaatc attttttatg tattcgaatt tcggtttttg tgtccatgat tctacagcac
     3001 ctacaggttt aacacctaaa gatacagcga cgtcagtggc accttgatat agcgtaacaa
     3061 cacgctttgg tttcccttta atttcagttg tacccattgc atgtttaatt gaagttgttt
     3121 ccttatcttt gttatcagat gattgtttat ttgaattccc actacatcct gctaaaacaa
     3181 gtaggaaagc aagcgtaaca acaagcattt taattacttt attcattgac taattagcct
     3241 ccttcgtgat gtatgacaat gagaatcatt atcacgattt agtatgaatt aaattttttc
     3301 ctaagtcaat aaaatattta tgatttacat gcaacttata attatttgac atataaatgc
     3361 ataaaaaata taatcctaat tacttgatag tgagaatcat tatcaattag gtaacacaca
     3421 atattataga attttaaatt tgaggaggaa gcgcttttga ttgaaaaaag tcaagcatgt
     3481 cacgattcat tgttagattc tgtagggcaa acacctatgg ttcaacttca tcaactattt
     3541 ccgaaacatg aagtgtttgc aaagttagag tatatgaatc ctggaggcag catgaaagat
     3601 cgacctgcca agtacatcat tgaacatggt attaaacatg gtttaatcac tgagaataca
     3661 catttaattg aaagtacttc tggtaattta ggcattgcgt tggcaatgat agctaaaatc
     3721 aagggattaa aactcacgtg tgttgttgat cctaaaatat caccaacaaa tttgaaaatt
     3781 attaaaagtt atggtgccaa tgtagaaatg gttgaagaac ctgatgcaca tgggggttat
     3841 ttaatgactc gtattgcaaa ggtgcaagaa ctgttagcca ctattgacga tgcatattgg
     3901 attaatcaat atgcgaatga gttaaattgg caatcccatt atcatggtgc aggcacagag
     3961 attgttgaaa caattaagca acctatagat tattttgtcg cgccagtcag cacgacaggt
     4021 agcattatgg gtatgagtag aaaaataaaa gaagtgcatc caaacgcaca aattgttgct
     4081 gttgatgcga aagggtcagt catttttggt gacaaaccta ttaatagaga attacctggt
     4141 atcggtgcta gtcgtgtacc cgaaatattg aatagatcag aaattaatca agtgatccat
     4201 gtagatgatt atcaatctgc tttgggctgt cgaaaactga ttgattatga aggcatattt
     4261 gccggaggtt caacaggttc gattattgca gcgattgagc agttgataac gtcaattgaa
     4321 gaaggtgcaa caattgtcac gattttacca gatcgaggcg atcgttactt agatttagtt
     4381 tattcagata catggttaga aaaaatgaaa tcaagacaag gagttaaatc agaatgaata
     4441 gagagatgtt gtatttaaat agatcagata ttgaacaagc gggaggtaat cattcacaag
     4501 tttatgtgga cgcattaaca gaagcattaa cagcccatgc gcacaatgat tttgtacaac
     4561 cgcttaagcc gtatttaaga caggatcctg aaaatggaca catcgcagat cgaattattg
     4621 caatgccaag tcatatcggt ggtgaacacg caatttcagg tattaagtgg ataggtagta
     4681 agcacgacaa tccatcgaaa cgtaatatgg agcgtgcaag tggcgtcatt attttgaatg
     4741 atccagaaac gaattatcca attgcagtta tggaagcaag tttaattagt agtatgcgta
     4801 ctgcagcagt ttcagtgatt gcagcaaagc atttggctaa aaaaggattt aaagacttaa
     4861 caatcattgg atgcgggcta atcggagaca agcaattaca aagtatgtta gagcaattcg
     4921 atcatattga acgcgtgttt gtttacgatc aattctctga agcatgtgca cgctttgttg
     4981 atagatggca acaacagcgt ccggaaatta attttattgc gacagaaaat gctaaagaag
     5041 cagtatcaaa tggtgaagta gtcattacat gtaccgtaac ggatcaacca tacattgaat
     5101 atgattggtt acaaaagggt gcatttatta gcaacatttc tatcatggat gtgcataaag
     5161 aagtctttat taaagctgac aaagtcgtag tagatgactg gtcacaatgt aatcgagaaa
     5221 agaaaactat taaccaattg gtgttagaag gtaaattcag caaagaagct cttcatgctg
     5281 aactaggaca acttgtgaca ggtgacatac caggacgtga agacgatgat gagatcatat
     5341 tacttaatcc gatgggtatg gctatcgaag atatttcaag tgcttatttt atttatcaac
     5401 aggcacaaca acaaaatatt gggacaacat tgaacctata ttaagaatgc gaggtgtctg
     5461 aacattgcag aatcatacag cagtcaatac agcacaagcg ataatattaa gagatttagt
     5521 tgatgcatta ttatttgaag atatagccgg aattgtatcg aatagtgaga ttactaaaga
     5581 aaacggacaa acgcttttga tatacgaacg tgaaacacaa caaataaaga tacctgttta
     5641 ttttagtgct ttaaatatgt ttcgttacga aagttcacaa ccaattacga tagagggaag
     5701 ggtgtctaag caacctttaa cggcagctga attttggcaa acaattgcta atatgaattg
     5761 tgatctaagt catgaatggg aagtggctcg cgttgaagaa ggactgacta ctgctgccac
     5821 acagcttgct aaacaattat cagaattaga tttagcgtca catccttttg tgatgtcaga
     5881 gcagtttgca agtttaaaag atcgtccatt tcatccatta gctaaagaaa aaagaggatt
     5941 aagagaagcg gattatcaag tgtatcaagc tgaattaaat caatcatttc ctttaatggt
     6001 tgcagcagtt aaaaagacac atatgattca tggcgatact gcaaatatcg atgaattaga
     6061 aaatttgaca gtacctataa aagaacaagc gacagacatg ttaaatgatc aagggttatc
     6121 aatagatgac tatgtactat ttccggtaca tccttggcaa tatcagcata ttctgccgaa
     6181 cgtctttgcg aaagagatta gtgaaaagtt ggttgtacta ttaccgttaa aatttggaga
     6241 ttatctgtcg tcttcaagta tgcgttcatt aattgatatt ggcgcaccgt ataaccatgt
     6301 caaagtacca tttgcaatgc agtcattagg cgcattaagg ctaacgccta cgcgttacat
     6361 gaaaaacgga gaacaagcag aacaattatt acgtcagctt atagaaaaag atgaagcact
     6421 agctaagtat gtcatggttt gtgatgaaac agcttggtgg tcatatatgg gtcaagataa
     6481 tgatattttc aaagatcaat taggtcatct aactgttcag ctaagaaagt atcccgaagt
     6541 gctagccaaa aatgatacgc aacagctagt gtcaatggca gcactcgcgg caaatgatcg
     6601 cactttatat caaatgattt gtggaaaaga taatatttct aaaaatgatg tcatgacgtt
     6661 atttgaagat atcgcgcaag tctttttaaa ggtaacacta tcatttatgc aatacggcgc
     6721 attaccagag ttgcatggtc aaaatatatt gttgtcattt gaagatggac gtgtacaaaa
     6781 atgcgtgtta cgtgatcatg atactgtcag aatttataaa ccatggctaa cagcacatca
     6841 gctttcattg ccgaagtatg tcgtcagaga agatacacct aatacgctaa ttaatgagga
     6901 tttggaaaca ttctttgctt attttcaaac attagctgta tcggtaaatc tatatgccat
     6961 tattgatgca attcaagatt tatttggtgt aagtgagcat gaacttatgt cgttgttaaa
     7021 acaaatttta aaaaatgaag tggcaactat ttcctgggtt acaactgatc agctagctgt
     7081 cagacacatt ttatttgata aacagacgtg gccattcaaa caaattttat taccattgct
     7141 atatcaacgt gatagtggtg gaggtagtat gccttcaggt ttaactaccg taccaaatcc
     7201 aatggtgaca tatgattaat cagtctatat ggcgcagtaa ctttcgcatt ttatggctca
     7261 gtcagtttat agcgattgct ggactgacag tacttgtgcc attattgcca atttatatgg
     7321 catcactaca aaatctatca gtcgtagaaa tacagttgtg gagtggtata gcgattgctg
     7381 ctccagctgt aacgacgatg atagcttcgc cgatatgggg gaagctaggt gataagatca
     7441 gccgaaaatg gatggtgtta agagcgttac ttggtttggc ggtatgctta tttttaatgg
     7501 cattgtgtac gacaccatta cagtttgtac ttgtgaggtt attgcaggga ctatttggtg
     7561 gtgttgttga tgcatcaagt gcgtttgcga gtgcagaggc gccagctgaa gatcgtggaa
     7621 aggtattagg aagactgcaa agttcagtca gcgcagggtc tcttgtgggg ccattaattg
     7681 gcggtgttac agcttcgata ttaggtttta gtgcgttact gatgagtatt gccgttatta
     7741 cttttattgt ctgtattttc ggtgcattaa aattgattga aacgacacat atgccaaaat
     7801 cacaaacacc aaatattaat aaaggtattc gccgttcatt tcaatgtcta ttatgcacac
     7861 aacaaacatg tcgatttatt atcgttggcg ttttagcaaa ctttgctatg tatggcatgc
     7921 taactgcatt atcaccactt gcttcatcag tgaatcatac agcgatagat gaccgtagtg
     7981 tgattggatt tttacagtcc gcattttgga cggcttcgat attaagcgcg cctttatggg
     8041 gacgctttaa tgataaatca tatgttaaat cagtatatat atttgccacg attgcatgtg
     8101 gttgtagtgc gatactgcaa ggtttagcga cgaatataga gtttttaatg gctgcaagaa
     8161 tacttcaagg attaacatat agtgcattga ttcaaagtgt catgtttgtt gtcgtgaatg
     8221 cgtgtcatca acaacttaaa ggcacatttg ttggaacgac gaacagtatg ttagttgttg
     8281 gtcaaattat tggcagtctt agtggcgctg ccattacaag ttatactaca ccagctacta
     8341 cgtttatcgt tatgggcgta gtatttgcag taagtagttt atttttaatt tgttcaacca
     8401 tcactaatca aatcaacgat cacacattaa tgaaattatg ggagttgaaa caaaaaagtg
     8461 caaaataaag aattaataca acatgcagcg tatgcggcta tcgaacgcat tttaaatgaa
     8521 tattttagag aagaaaattt atatcaagta ccacctcaaa atcatcaatg gtctatacaa
     8581 ttatcagagc tcgaaacttt aacgggtgaa tttcgctatt ggtctgcgat ggggcatcat
     8641 atgtatcatc cagaggtatg gcttatcgat ggaaaaagta aaaaaataac aacttataaa
     8701 gaagcaattg cgcgtatttt gcaacatatg gctcaaagtg cagataatca aacggcagtg
     8761 caacaacata tggcgcaaat tatgtctgac atcgataata gcattcatcg cacggcacgt
     8821 tatttgcaaa gtaacacaat agactacgta gaggatcgtt atatcgtttc agaacaatct
     8881 ttatacttag gtcatccatt tcatccgact cctaagagtg caagtgggtt ttcagaagca
     8941 gatttagaga aatatgcacc cgaatgtcat acatcattcc aattgcatta tttagctgtg
     9001 catcaagatg ttctgctcac gcgctatgta gaaggtaaag aagatcaggt tgagaaagtg
     9061 ttgtatcaat tagcagacat agatatatca gagataccca aagattttat tttattacca
     9121 acacatcctt atcaaatcaa tgtgttgcga cagcatccac agtatatgca atatagtgaa
     9181 caaggtttaa taaaagacct tggcgtttcc ggtgattcag tgtacccgac gtcttcggtt
     9241 agaactgtat tttcaaaagc attaaacatt tatttaaaat taccgataca cgttaaaatc
     9301 actaatttta tacgtacgaa tgaccttgaa cagattgaac ggacaattga tgccgcgcaa
     9361 gttatcgcat cagtcaaaga tgaggttgaa acaccccatt ttaaattgat gtttgaagaa
     9421 ggatatcgtg cattgttacc gaatccatta gggcaaacag ttgaacctga aatggattta
     9481 ttaacaaata gtgccatgat tgttcgtgaa gggataccga attaccatgc tgataaagat
     9541 attcatgtat tggcgtcatt atttgaaacg atgcctgatt caccgatgtc taagttatca
     9601 caagtgattg agcaaagtgg tttagcacca gaagcatggc ttgaatgtta tttgaatcgt
     9661 acattattgc cgatattaaa gctgtttagt aacacaggca ttagtctaga agcacatgta
     9721 caaaatacat taattgaatt aaaagatggc atacccgacg tatgctttgt cagagatctt
     9781 gaaggcattt gtctatctag aacgattgct actgaaaaac agcttgtgcc aaatgttgtg
     9841 gcagcatcaa gccctgttgt atatgcacat gatgaagcat ggcatcgtct taaatattac
     9901 gttgtagtaa atcacttagg acatttagta tcaactattg gtaaagcgac tagaaatgaa
     9961 gttgtgttat ggcaacttgt agcgcatcgt cttatgactt ggaaaaaaga atacgcgaat
    10021 aacgcagtat ttgttgactg tgtagaagat ttatatcaaa cgccgaccat tgcggctaaa
    10081 gcgaatttga tgagtaaatt gaatgattgt ggtgcaaacc ctatttatac acatatacca
    10141 aatccaattt gtcataacaa ggaggtatcg tattgtgaat caaacaattc ttaatcgtgt
    10201 aaagactaga gtgatgcacc aactggtatc atcacttatt tatgagaata ttgttgtgta
    10261 taaagcgtca tatcaagacg gtgtcggtca ttttacaata gaaggacatg attcagagta
    10321 tcgttttact gctgaaaaga cacatagctt tgatcgtata cgtatcacat caccaattga
    10381 gcgtgtcgta ggagatgagg cagatacaac aacagactat acacaattat tgagagaggt
    10441 tgtatttaca tttcctaaaa atgatgaaaa gctagaacaa tttattgtcg agttattaca
    10501 gacagaatta aaagatacgc aaagtatgca gtatcgagaa tcaaacccac cagcaacacc
    10561 tgagacattt aacgactatg aattttatgc gatggaaggg catcagtatc atccaagtta
    10621 caaatcacgt ttaggattta cgttgagtga taatttgaaa tttggtcctg attttgtacc
    10681 aaacgttaaa ctgcagtggt tagctatcga caaagataaa gtagaaacga cggtatcaag
    10741 aaatgttgta gttaacgaaa tgttacgtca acaagttggc gataagactt atgaacattt
    10801 tgtacagcag attgaagcat ctggcaaaca tgtaaatgat gttgagatga tacctgtaca
    10861 cccatggcag tttgaacatg tcatccaagt tgatttggct gaagaaaggc ttaatggcac
    10921 agtactatgg ttaggggaaa gtgatgagct atatcaccct caacaatcga ttcgtacgat
    10981 gtcgccaata gacacgacaa aatattattt aaaggtacca ataagtataa cgaacacttc
    11041 aacgaaacga gtgttggcgc ctcatacaat tgaaaatgca gcgcaaatta cggattggtt
    11101 aaagcagata cagcaacaag atatgtattt aaaagatgaa ttaaagacag tttttctagg
    11161 ggaagtctta ggacagtctt atttaaatac acaactttcg ccttataaac aaactcaagt
    11221 ttatggtgcg ttaggtgtta tatggcgtga aaatatatat catatgttaa tcgatgaaga
    11281 ggatgcgata ccatttaatg cactttatgc aagtgataag gatggtgtac cattcattga
    11341 aaattggatt aaacaatatg gttctgaagc ttggacaaag caatttttag ctgtagcgat
    11401 tcgtccaatg attcatatgc tttattatca cggtattgcc tttgaatcgc atgcacaaaa
    11461 tatgatgctc attcatgaaa atggttggcc tacacgtatt gccttaaaag atttccatga
    11521 tggtgttcgt tttaagcgtg agcatttaag tgaagctgct tcacacctga cattaaagcc
    11581 aatgccagaa gcacataaaa aagtgaatag taattcattt attgaaacag atgacgaacg
    11641 tttagtacgc gactttttac atgatgcatt tttctttatt aatatcgccg aaatcatctt
    11701 atttattgaa aagcaatatg gtatcgatga ggagctgcaa tggcaatggg ttaaaggcat
    11761 catcgaggcg tatcaagaag catttccaga gttgaataac tatcaacatt tcgatttgtt
    11821 tgaacctacg attcaagttg aaaagttaac gacacgtcga ttattaagtg actccgagtt
    11881 aagaattcat catgttacaa atccattagg tgtaggaggt atcaatgatg caacaactat
    11941 ctctgaaaca tagattaaac aatggtgatt cagtttatgg catttttaat tctataccgg
    12001 acccattgat gatcgaggtt atcgcagcaa gcgggtatga ctttgttgtg attgatacag
    12061 aacacgtggc gattaatgat gagacactag cgcatttaat tcgtgcagct gaagcagcgc
    12121 atattatacc aattgtacgt gtcactgcag tgatagatag agatatcatt aaagtgttgg
    12181 atatgggtgc gagaggtatt attgtgccac acgttaaaga tcgtgagaca gttgagcata
    12241 ttgtgaaatt aagtcgttat tacccgcaag gattaagaag tttgaatggt ggtcgcatgg
    12301 caagatttgg acgtacacca ttacttgatg caatggagat ggctaatgag catattatgg
    12361 tgattgccat gatagaagat gttgaagggg ttatggccat tgacgatata gcacaagtcg
    12421 aaggtttaga catgatagtc gaaggtgccg cagatttatc gcagtcactt ggcataccat
    12481 ggcaaacgcg tgatgatcaa gtaacatcac atgttcaaca tatttttgaa gttgtgaatg
    12541 cacatggtaa acatttttgt gcattaccac gtgaagatga agatattgca aaatggcagg
    12601 cacaaggtgt acaaacattt attttaggtg atgatcgcgg aaaaatatat cgccatttaa
    12661 gtgcatctct agcgacgtct aaacagaaag gggatgaagg ctaatgcgta tagttcaacc
    12721 tgttattgaa caattaaaag cacaatctca tccagtttgt cattatatct atgatttagt
    12781 cggactggaa catcatttgc aacatattac atcgtcattg ccgagtaatt gtcaaatgta
    12841 ctatgcaatg aaagcaaata gtgaacgaaa aatcctagat acaattagtc agtatgttga
    12901 aggattcgaa gttgcatctc aaggtgaaat agcaaaaggt cttgctttta aaccagcaaa
    12961 tcatattatt tttggtggcc ctggtaagac agacgaggaa ctaagatatg cagtaagtga
    13021 aggtgttcag cgtattcatg ttgaaagtat gcatgaatta caacggctaa atgccatctt
    13081 agaagatgaa gataagacac aacacatttt attgcgtgtt aatttagcag gaccatttcc
    13141 caatgcaacg ttgcatatgg caggacgccc aacacaattt ggtatttctg aagacgaagt
    13201 tgatgatgtc attgaagctg cgctcgcaat gccaaagatt catctagatg gatttcattt
    13261 tcattctatt tctaacaatt tagactcgaa tttacatgtc gatgtagtga aactttattt
    13321 taaaaaagca aaggcatggt ctgaaaaaca tcgatttcca ctcaaacata tcaatcttgg
    13381 tggtggcata ggcgttaact atgcagattt aactaaccaa tttgaatggg ataattttgt
    13441 agaacgtttt aaaacactta tcgttgagca agaaatggaa gatgtgacat tgaactttga
    13501 atgtgggcgc tttattgtgg cacatattgg ttactatgtg acagaagtgc tagacattaa
    13561 gaaagtacat ggtgcttggt atgccatatt aagaggaggt acgcaacaat ttagactgcc
    13621 ggtatcttgg caacataacc atccttttga catttatcgc tataaggaca atccatattc
    13681 atttgaaaaa gtttcaattt cgagacagga cacaacgtta gtcggtcaat tatgtacacc
    13741 gaaagatgtc tttgctagag aagtacagat agacgcaatc agtacaggcg acgttattgt
    13801 tttcaaatat gcaggtgcat acggatggtc tatttcacat cacgatttct taagccatcc
    13861 acatcctgaa tttatttatt taacgcaaac aaaggaggat gaataactat tgaatcatat
    13921 tcatgaacat ttaaaattgg taccagtaga taagattgat cttcacgaaa cattcgaacc
    13981 tttaagattg gaaaaaacga aaagtagtat tgaagcagat gattttatac gtcatcctat
    14041 tttagtgaca gcgatgcaac atggtagata tatggttata gatggtgtgc atcggtatac
    14101 aagtttgaaa gcgttaggat gtaagaaagt tccagtgcaa gaaatccatg aaacacaata
    14161 ttcaattagt acatggcaac ataaagttcc atttggtgtg tggtgggaaa cgttacaaca
    14221 agaacatcgc ttgccatgga ctactgagac aagacaagaa gcgccattta ttacaatgtg
    14281 tcatggtgat acagaacaat atttgtatac aaaagattta ggcgaagcac attttcaagt
    14341 atgggaaaag gttgtcgcaa gttatagtgg ttgttgttct gtagagagaa ttgcacaagg
    14401 tacatatcct tgtctttctc aacaagatgt actcatgaag tatcagccat tgagttataa
    14461 ggaaattgaa gcggttgttc ataaagggga aactgtgcca gcaggtgtga cacgctttaa
    14521 tatttcagga cgatgtctta atcttcaagt accactggca ttacttaaac aagatgatga
    14581 tgttgaacaa ctgcgcaatt ggaagcagtt tttagcagat aagtttgcca atatgagatg
    14641 ctatactgaa aaagtatact tggtggagca atagttttac tgtgatgttg agggaaatat
    14701 gatgatttag cgtattgata gcgaaaatat aataaaacaa tatagtgtgg agaacttttg
    14761 atattttata aatattgaag ttctccattt ttgtattttg catataaaaa ttaaataaaa
    14821 taaggtatat taaggtaaag tataaatttt aaataaatgg ggaatgagta tgagctcaat
    14881 tataggaaaa atagcaattt ggataggcat cgtagctcaa atatatttta gtgtcgtttt
    14941 tgttaggatg atatctatta atattgctgg aggatctgat tacgaaacaa tttttttatt
    15001 aggattaata ttggctcttt tcactgtttt accaaccatc tttactgcga tttatatgga
    15061 aagttactct gtaatcggag gtgcactttt tattgtttat gctattattg cactgtgttt
    15121 atataatttc ctttcgtcaa ttttatggct gattggtggt attttgctga tttggaataa
    15181 atactcaaaa gatgaatcga cagacgaaaa tgaaaaagtt gatattgaaa gtacagagaa
    15241 tcaatttgaa tctaaagata aaatcactaa agaataaaga gaatatttaa ggtaaagtat
    15301 aaattttaaa taaatgggga atagacatgg aaaaaaatgt agaaaaatca ttcataaaga
    15361 taggtttata ttttcaaata gcttatatag tactcatggc tataacttta tgtgggtttg
    15421 taatttgcta tggactaatt ttcggccttt tctatttatt atcaggtagc agagctgatt
    15481 atttaatagt aacaatagtt atatcggcaa taatttctat atttgtaatt atactttcaa
    15541 tcgtacctgt catcgtattg gcatctgact tatttaaaga aaggatttca aaaggtgtca
    15601 tattaattgt attggctatt atcgctttag tattatgcaa ctttgtatct gcaatactct
    15661 ggtttgtttc agccatatct attttaggta gaaaaaaatt agtagctgca gcagatacta
    15721 ccactattca aaaaagtaaa gggaacgcaa atcaagcatc acataaagac acgtgtaaaa
    15781 aggaacttga tagtcaagac atgatggaac atcctgaggt taaaaatccc acgactaaaa
    15841 accttgaagg atttaacgaa gaaatacata aagatgaagc tacaactaaa gttgtcagtg
    15901 ataacacgga accgcctatt gaatcaaaag accatgtctc gaaaaaagat tgatgacaaa
    15961 ctaatcgaga gacttaaaaa aataatattc aacataagaa cttttaaaac gacatttaaa
    16021 cgcattgcca atcactaatg gtagtgcgtt taactatacc ttaaatatct gaatattttg
    16081 ttaaatggag ctacctttgt tgtactattc aaatgaagag gagtaaaatg taattaaagg
    16141 aaagaaattt gaggagtgat ctttatgaca aacaacaaag tagcattagt aactggcgga
    16201 gcacaaggga ttggttttaa aattgcagaa cgtttagtgg aagatggttt caaagtagca
    16261 gttgttgatt tcaatgaaga aggggcaaaa gcagctgcac ttaaattatc aagtgatggt
    16321 acaaaagcta ttgctatcaa agcagatgta tcaaaccgtg atgatgtatt taacgcagta
    16381 agacaaactg ccgcgcaatt tggcgatttc catgtcatgg ttaacaatgc cggccttgga
    16441 ccaacaacac caatcgatac aattactgaa gaacagttta aaacagtata tggcgtgaac
    16501 gttgcaggtg tgctatgggg tattcaagcc gcacatgaac aatttaaaaa attcaatcat
    16561 ggcggtaaaa ttatcaatgc aacatctcaa gcaggcgttg agggtaaccc aggcttgtct
    16621 ttatattgca gtacaaaatt cgcagtgcga ggtttaacac aagtagccgc acaagattta
    16681 gcgtctgaag gtattactgt gaatgcattc gcacctggta tcgttcaaac accaatgatg
    16741 gaaagtatcg cagtggcaac agccgaagaa gcaggtaaac ctgaagcatg gggttgggaa
    16801 caatttacaa gtcagattgc tttgggcaga gtttctcaac cagaagatgt ttcaaatgta
    16861 gtgagcttct tagctggtaa agactctgat tacattactg gacaaacaat tattgtagat
    16921 ggtggtatga gattccgtta ataatcatcc actaatgata aataaatcct tattgttaag
    16981 tttaatcact tagcagtaag gattttttag tgcacttaga agggagtgta ttggtagaaa
    17041 attaataagc gaagttctta agtgagttat gatgtcacag tctaatgcat cagttgaaag
    17101 cattattagt attaacacac ccaagatatt ataaaacatc acaaaaacac cactatctaa
    17161 tttatctcaa taaaaattca caaagttatc tcattttatt tttataaata aaaaatatcg
    17221 ataaaaagct tacaatactt tatgttttta tgatatattt ttaatgtata aatgaggtgg
    17281 aagatttgga aagagttttg ataactggtg gggctggttt tattgggtcg catttagtag
    17341 atgatttaca acaagattat gatgtttatg ttctagataa ctatagaaca ggtaaacgag
    17401 aaaatattaa aagtttggct gacgatcatg tgtttgaatt agatattcgt gaatatgatg
    17461 cagttgaaca aatcatgaag acatatcaat ttgattatgt tattcattta gcagcattag
    17521 ttagtgttgc tgagtcggtt gagaaaccta tcttatctca agaaataaac gtcgtagcaa
    17581 cattaagatt gttagaaatc attaaaaaat ataataatca tataaaacgt tttatctttg
    17641 cttcgtcagc agctgtttat ggtgatcttc ctgatttgcc taaaagtgat caatcattaa
    17701 tcttaccatt atcaccatat gcaatagata aatattacgg cgaacggacg acattaaatt
    17761 attgttcgtt atataacata ccaacagcgg ttgttaaatt ttttaatgta tttgggccaa
    17821 gacaggatcc taagtcacaa tattcaggtg tgatttcaaa gatgttcgat tcatttgagc
    17881 ataacaagcc atttacattt tttggtgacg gactgcaaac tagagatttt gtatatgtat
    17941 atgatgttgt tcaatctgta cgcttaatta tggaacacaa agatgcaatt ggacacggtt
    18001 ataacattgg tacaggcact tttactaatt tattagaggt ttatcgtatt attggtgaat
    18061 tatatggaaa atcagtcgag catgaattta aagaagcacg aaaaggagat attaagcatt
    18121 cttatgcaga tatttctaac ttaaaggcat taggatttgt tcctaaatat acagtagaaa
    18181 caggtttaaa ggattacttt aattttgagg tagataatat tgaagaagtt acagctaaag
    18241 aagtggaaat gtcgtgaaaa tgacattgaa gctgtccata ataataaggg ttatgcctat
    18301 caaagaaaat tagacaaact agaagaagtg agaaaaagct attacccaat taaacgtgcg
    18361 attgacttaa ttttaagcat tgttttatta tttttaactt taccgattat ggttatattc
    18421 gccattgcta tcgtcataga ttcgccagga aaccctattt atagtcaggt tagagttggg
    18481 aagatgggta aattaattaa aatatacaaa ttacgttcga tgtgcaaaaa cgcagagaaa
    18541 aacggtgcgc aatgggctga taaagatgat gatcgtataa caaatgtcgg gaagtttatt
    18601 cgtaaaacac gcattgatga attaccacaa ctaattaatg ttgttaaagg ggaaatgagt
    18661 tttattggac cacgcccgga acgtccggaa tttgtagaat tatttagttc agaagtgata
    18721 ggtttcgagc aaagatgtct tgttacacca gggttaacag gacttgcgca aattcaaggt
    18781 ggatatgact taacaccgca acaaaaactg aaatatgaca tgaaatatat acataaaggt
    18841 agtttaatga tggaactata tatatcaatt agaacattga tggttgttat tacaggggaa
    18901 ggctcaaggt agtcttaatt tacttaataa gttcaaataa aagttatatt ttaaagattg
    18961 tgaccaattg ttacagtata acgaggaatc ccttgagaca gtatcaaatg gcattaagaa
    19021 atatgtgcca tcattgattt gcatggctat aaatactatt catctgatga gatagccatg
    19081 ttaagaaatt gaaagtatag cattaaaggg gtttgtaaca gttgaaaatt atatattgta
    19141 ttactaaagc agacaatggt ggtgcacaaa cacatctcat tcaactcgcc aaccattttt
    19201 gcgtacacaa tgatgtttat gtcattgtag gcaatcatgg accaatgatt gaacaactag
    19261 atgcaagagt taatgtaatt attatcgaac atttagtagg tccaattgac tttaaacaag
    19321 atattttagc tgtcaaagtg ttagcacagt tattctcgaa aattaaacct gatgttatcc
    19381 atttacattc ttccaaagct ggaacggtcg gacgaattgc gaagttcatt tcgaaatcga
    19441 aagacacacg tatagttttt actgcacatg gatgggcttt tacagagggt gttaaaccag
    19501 ctaaaaaatt tctatattta gttatcgaaa aattaatgtc acttattaca gatagcatta
    19561 tttgtgtttc agatttcgat aaacagttag cgttaaaata tcgatttaat cgattgaaat
    19621 taaccacaat acataatggt attgcagatg ttcccgctgt taagcaaacg ctaaaaagcc
    19681 aatcacataa caatattggc gaagtagttg gaatgttgcc taataaacaa gatttacaga
    19741 ttaatgcccc gacaaagcat caatttgtta tgattgcaag atttgcttat ccaaaattgc
    19801 cacaaaatct aatcgcggca atagagatat tgaaattaca taacagtaat catgcgcatt
    19861 ttacatttat aggcgatgga cctacattaa atgattgtca gcaacaagtt gtacaagctg
    19921 ggttagaaaa tgaagtcaca tttttgggca atgtcattaa tgcgagtcat ttattatcac
    19981 aatacgatac gtttatttta ataagtaagc atgaaggttt gccaattagc attatagaag
    20041 ctatggctac aggtttgcct gttatagcca gtcatgttgg cggtatttca gaattagtag
    20101 ctgataatgg tatatgtatg atgaacaacc aacccgaaac tattgctaaa gtcctggaaa
    20161 aatatttaat agacagtgat tacatcaaaa tgagtaatca atctagaaaa cgttatttag
    20221 aatgttttac tgaggagaaa atgattaaag aagtggaaga cgtttataat ggaaaatcaa
    20281 cacaatagta aattactaac attgttactt atcggtttag cggtttttat tcagcaatct
    20341 tcggttattg ccggtgtgaa tgtttctata gctgacttta tcacattact aatattagtt
    20401 tatttactgt ttttcgctaa ccatttatta aaggcaaatc attttttaca gtttttcatt
    20461 attttgtata catatcgtat gattattacg ctttgtttgc tattttttga tgatttgata
    20521 tttattacgg ttaaggaagt tcttgcatct acagttaaat atgcatttgt agtcatttat
    20581 ttctatttag ggatgatcat ctttaagtta ggtaatagca aaaaagtgat cgttacctct
    20641 tatattataa gcagtgtgac tataggtcta ttttgtatta tagctggttt gaacaagtcc
    20701 cctttactaa tgaaattgtt atattttgat gaaatacgtt caaaaggatt aatgaatgac
    20761 cctaactatt tcgcgatgac acagattatt acattggtac ttgcttacaa gtatattcat
    20821 aattacatat tcaaggtcct tgcatgtggt attttgctat ggtctttaac tacaacgggg
    20881 tctaagactg cgtttatcat attaatcgtc ttagccattt atttctttat taaaaagtta
    20941 tttagtagaa atgcggtaag tgttgtgagt atgtcagtga ttatgctgat attactttgt
    21001 tttacctttt ataatatcaa ctactattta ttccaattaa gcgaccttga tgccttaccg
    21061 tcattagatc gaatggcgtc tatttttgaa gagggctttg catcattaaa tgatagtggg
    21121 tctgagcgaa gtgttgtatg gataaatgcc atttcagtaa ttaaatatac actaggtttt
    21181 ggtgtcggat tagtggatta tgtacatatt ggctcgcaaa ttaatggtat tttacttgtt
    21241 gcccataata catatttgca gatctttgcg gaatggggca ttttattcgg tgcattattt
    21301 atcatattta tgctttattt actgtttgaa ttatttagat ttaacatttc tgggaaaaat
    21361 gtaacagcaa ttgttgtaat gttgacgatg ctgatttact ttttaacagt atcatttaat
    21421 aactcaagat atgtcgcttt tattttagga attatcgtct ttattgttca atatgaaaag
    21481 atggaaaggg atcgtaatga agagtgattc actaaaagaa aatattattt atcaagggct
    21541 ataccaattg attagaacga tgacaccact gattacaata cccattattt cacgtgcatt
    21601 tggtcccagt ggtgtgggta ttgtttcatt ttctttcaat atcgtgcaat actttttgat
    21661 gattgcaagt gttggcgttc agttatattt taatagagtt atcgcgaagt ccgttaacga
    21721 caaacggcaa ttgtcacagc agttttggga tatctttgtc agtaaattat ttttagcgtt
    21781 aacagttttt gcgatgtata tggtcgtaat tactatattt attgatgatt actatcttat
    21841 tttcctacta caaggaatct atattatagg tgcagcactc gatatttcat ggttttatgc
    21901 tggaactgaa aagtttaaaa ttcctagcct cagtaatatt gttgcgtctg gtattgtatt
    21961 aagtgtagtt gttatttttg tcaaagatca atcagattta tcattgtatg tatttactat
    22021 tgctattgtg acggtattaa accaattacc tttgtttatc tatttaaaac gatacattag
    22081 ctttgtttcg gttaattgga tacacgtctg gcaattgttt cgttcgtcat tagcatactt
    22141 attaccaaat ggacagctca acttatatac tagtatttct tgcgttgttc ttggtttagt
    22201 aggtacatac caacaagttg gtatcttttc taacgcattt aatattttaa cggtcgcaat
    22261 cataatgatt aatacatttg atcttgtaat gattccgcgt attaccaaaa tgtctatcca
    22321 gcaatcacat agtttaacta aaacgttagc taataatatg aatattcaat tgatattaac
    22381 aatacctatg gtctttggtt taattgcaat tatgccatca ttttatttat ggttctttgg
    22441 tgaggaattc gcatcaactg tcccattgat gaccatttta gcgatacttg tattaatcat
    22501 tcctttaaat atgttgataa gcaggcaata tttattaata gtgaataaaa taagattata
    22561 taatgcgtca attactattg gtgcagtgat aaacctagta ttatgtatta ttttgatata
    22621 tttttatgga atttacggtg ctgctattgc gcgtttaatt acagagtttt tcttgctcat
    22681 ttggcgattt attgatatta ctaaaatcaa tgtgaagttg aatattgtaa gtacgattca
    22741 atgtgtcatt gctgctgtta tgatgtttat tgtgcttggt gtggtcaatc attatttgcc
    22801 ccctacaatg tacgctacgc tgctattaat tgcgattggt atagtagttt atcttttatt
    22861 aatgatgact atgaaaaatc aatacgtatg gcaaatattg aggcatcttc gacataaaac
    22921 aatttaagta ccggtaatgc tatactttag aaaattaaga ttaagaagaa aaggcaattt
    22981 cttattgaaa aatggaagtt gtctttttta attctcttta aaagcgggaa acaaaagcag
    23041 ttaaatgcct ttttgcattc aatattaaat attatatcaa tttcgaatat ttaaatttta
    23101 tataattgga tataacaaat aaataataat tattgcaaaa cacacccgaa attaattatt
    23161 ataaaagtat attcataaaa ggaggaatat acttatggca tttaaattac caaatttacc
    23221 atatgcatat gatgcattgg aaccatatat agatcaaaga acaatggagt ttcatcacga
    23281 caaacatcac aatacgtacg tgacgaaatt aaacgcaaca gttgaaggaa cagagttaga
    23341 gcatcaatca ctagcggata tgattgctaa cttagacaag gtaccggaag cgatgaggat
    23401 gtcagtccgt aataatggcg gtggtcattt taaccattca ttattctggg aaatactatc
    23461 acctaattct gaagaaaaag gtggcgtaat agatgacatc aaagcgcagt ggggcacttt
    23521 agatgaattt aaaaatgaat ttgcaaataa agcaacaaca ttatttggat caggttggac
    23581 ttggttagtt gttaatgatg gcaaattaga aattgtgaca acgccaaacc aagataatcc
    23641 attaacagaa ggcaaaacac caatcttact atttgatgtt tgggagcatg cctactatct
    23701 gaaatatcaa aataaacgtc cagactatat gactgcattt tggaacattg ttaactggaa
    23761 aaaagttgat gaattatacc aagcagcaaa ataatataac ttaatataat tgaggtggag
    23821 catctacaag gtgttccact tttttgtact taatatcttt acttatttgt ttttgtgtaa
    23881 tgggatagca cgtaaaagtg gaaaaataat aagataaatt tcaactcgaa ataccttatt
    23941 attgttgact ataacgatga cctaaatatt atatacatta gacaacacta ttaattaatc
    24001 aacatattga cattaaataa attgacaaaa taagtaatta ttgtgaatct aaagtgaaat
    24061 ttttataaaa aaatgtaatg attcaaaaat tttgttgcat ttcttttgta atcgtatgat
    24121 aatgtaaatg taatcaaatt gtaatataag gggacaagac aatgaaaaaa ttagcaacag
    24181 taggttcttt aattgtaaca agcactttag tattctcaag tatgcctttt caaaatgcgc
    24241 atgccgacac aacttcaatg aatgtgtcga ataaacaaag ccaaaatgta caaaatcatc
    24301 gtccttatgg cggagtagta ccacaaggaa tgacgcaagc acaatatact gaattagaga
    24361 aagctttacc ccaattaagc gctggcagta atatgcaaga ctataatatg aaattgtatg
    24421 atgcgacgca aaatattgct gataaataca atgtgataat tacaactaat gtaggggtat
    24481 ttaaaccaca tgctgttaga gatatgaatg gccatgcgtt acctttaaca aaagatggca
    24541 atttttatca aacgaatgta gatgcaaatg gtgttaatca tggtggtagt gaaatggtgc
    24601 aaaataaaac aggtcatatg agtcaacaag gccatatgaa tcagaacaca cacatgaacc
    24661 aacagccaca catgcaacaa ggtcatatgc aatcatcaaa ccatcaaatg atgagtccaa
    24721 aagcaaatat gcattcatca aatcatcaaa tgaaccaaag taacaaaaaa gttttaccag
    24781 ctgctggtga aagtatgaca tcaagtattc ttactgcaag tattgccgca ctactattag
    24841 tatctgggtt attcttagca tttagacgac gttcaacaaa taaataaaca taatacgatt
    24901 aataatagaa aaatcgtgtg attatctgag cgagcctagg acataaatca atgtcctagg
    24961 ctcgctaatg ttatattggc agtagttgac tgaatgaaat tgcgcttgta acaagctttt
    25021 ccatttctta ccaactccct aaatagtcat caaaaaattt cttatatttt aataattttt
    25081 aataatccga ttgtcttata cgtgtcagtg ttaattcaga tatttcctgt ggaatatacc
    25141 acttattaat cataattgga taaggtgttt gtgcgtacag tgtttcaata atcagccaac
    25201 aatgtgtatc accatcaaac acgtgactat gatttttgaa gtggggcgct ttggtaatag
    25261 acatttttaa atctgattga tatgcattgc tataaatcgt ttgctcaacg aatgtcttca
    25321 tgtcgtcttc gttttgtgta ttcactttaa atgtgtcaat gacatttaac ggtataaagg
    25381 taaagcaaaa tgcatcagct tgcttagaat gattgtcctt tttttgataa tagcgttcca
    25441 ttgcaatgac ggcagaagga tggtttgcaa acaaatgatt tgtatattca ctttctaaat
    25501 caacacgata attaattgat gacatagata cgcgagctag caatatttga tcaagtggat
    25561 gcttaaattg atccatactt gaagcgtgtt gggcatttgt ttgtggaata acaaagtgtc
    25621 ccttccctct tgtactctct acgatgccat cttcggctaa caattttata gcttggcgca
    25681 aagtcatacg actgacatca aagcgcgcac aaagttcctt ttcagtaggt aatgcatggc
    25741 cactcggata ttttcctatt tgaatttctt tatataacgt attataaatc gttaaaaatt
    25801 ttggttgtgt ttgcgtcacg tagacaacct ccataaagtt acttaatcac tctcatcata
    25861 caataatttt tactcaaatt ggaaaaatta taaaaattaa atatagatag gctttgaaaa
    25921 ttagttttat acaaggttag tagctgtaac tgtaaaatgt tcttaatatt gtcaaaatgt
    25981 aatgcttgaa agcgctttta aaaaatatta ttatatacat ggttagacaa atagacaaat
    26041 cactatacaa atattgggag gaatatttta tgaaatcaac accacacatt aaaccaatga
    26101 atgacgtcga aattgcagaa acggttctat tgccaggaga tccgttaaga gctaagttca
    26161 ttgcagaaac ttatttggat gatgtggaac agttcaatac agtgcgaaac atgtttggtt
    26221 ttaccggaac atataaaggt aaaaaagttt ctgtcatggg ttcaggtatg ggtatgccat
    26281 ctattggcat ttactcttat gaattaattc atacatttgg ttgtaaaaaa ttaattcgcg
    26341 ttggctcttg tggcgcgatg caagaaaaca ttgatttata tgatgtgatt attgcacaag
    26401 gtgcctctac tgattcaaat tacgttcaac aatatcaatt accaggtcat tttgcgccaa
    26461 ttgcttctta tcaattatta gaaaaagcag ttgaaacagc acgtgacaaa ggtgtacgtc
    26521 atcatgtagg taatgtgtta tcaagtgata ttttctataa cgcggataca acagcgagtg
    26581 aacgttggat gcgtatgggt attttaggtg tagaaatgga atcagctgca ttatacatga
    26641 atgcaattta cgctggtgtc gaagcattag gtgtgttcac agtgagcgat catttaattc
    26701 atgaaacgtc aacaacacct gaggaaaggg aacgtgcatt tacagatatg attgaaattg
    26761 cactgtcatt ggtgtagatg attatgaatg ttgaatattc taaaataaag aaagcagtac
    26821 ctattttatt attcttattt gtattcagtt tggttataga caactcattt aaattgattt
    26881 ctgtagccat tgctgatgac ttaaacatat ctgtaacgac agtaagttgg caagcgacat
    26941 tagccggttt agtaattggt attggcgctg tagtatacgc ttcattatct gatgccatta
    27001 gtatacgcac actatttatt tatggcgtga tattaatcat tatcggatca attattggtt
    27061 acattttcca acatcaattc ccattacttt tagttggacg tattattcaa actgccggtt
    27121 tagctgctgc agagacatta tatgtgatat atgttgcaaa gtatctttct aaagaggacc
    27181 agaagactta ccttggctta agtacgagca gttattcctt gtcattagtt atcggtacat
    27241 tatcaggtgg atttatttct acgtatttac actggacaaa tatgttttta attgcattaa
    27301 tcgtagtatt tacgttgcca ttcctattta aattattacc aaaagaaaat aatacgaata
    27361 aagctcattt agattttgtt ggcttaattc tagtggcaac tattgctaca acagtcatgc
    27421 tgtttattac gaactttaat tggttatata tgattggtgc cttaattgcg attatcgttt
    27481 ttgcgctata tattaaaaat gcgcaacgtc cattagtaaa taaatcattt ttccaaaata
    27541 aacgttatgc ttcattttta tttatagtat ttgtaatgta tgctatccaa ttgggttata
    27601 tttttacgtt cccattcata atggagcaaa tttatcatct gcaactagac acaacatcac
    27661 tgttattagt accgggttat atagtagcag tcattgttgg tgcactaagt ggtaaaatcg
    27721 gcgaatatct gaattcaaaa caagcgatta tcacagcaat tattttaata gcactgagct
    27781 tgattttacc tgcatttgca gtaggtaatc acatttcaat cttcgtcatt tctatgatat
    27841 tctttgcagg tagctttgct ttaatgtatg cacctttact taacgaagcc attaaaacaa
    27901 tagatcttaa tatgacaggt gtggctattg gtttttataa tttaattatt aatgtggcgg
    27961 tatctgtagg tattgcgatt gctgcggctc taatcgattt taaagcatta aatttcccag
    28021 gcaatgatgc attaagttca catttcggta ttattttaat tattttaggt ttaatgagta
    28081 ttgtcggatt agttttattc gtcagcttaa atcgttggac acaatctgaa aaataaatag
    28141 atataaattc gcgagatata ttcgtattta tagtaaaatt aaataaagag attatataac
    28201 acgaggagta gtaagtatga aatttgagaa atatat
//
LOCUS       DAKTLH010000021        27138 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.021, whole genome shotgun sequence.
ACCESSION   DAKTLH010000021 DAKTLH010000000
VERSION     DAKTLH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 27138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27138
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.021"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            18..1373
                     /gene="brnQ"
                     /locus_tag="O0394_000795"
     CDS             18..1373
                     /gene="brnQ"
                     /locus_tag="O0394_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCY0498895.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKLIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQ
                     A"
     gene            1626..2810
                     /gene="rocD"
                     /locus_tag="O0394_000796"
     CDS             1626..2810
                     /gene="rocD"
                     /locus_tag="O0394_000796"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCY0498896.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVF
                     KN"
     gene            2849..3880
                     /locus_tag="O0394_000797"
     CDS             2849..3880
                     /locus_tag="O0394_000797"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCY0498897.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLNQSPVYP"
     gene            3892..5133
                     /gene="argJ"
                     /locus_tag="O0394_000798"
     CDS             3892..5133
                     /gene="argJ"
                     /locus_tag="O0394_000798"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HCY0498898.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            5149..5913
                     /gene="argB"
                     /locus_tag="O0394_000799"
     CDS             5149..5913
                     /gene="argB"
                     /locus_tag="O0394_000799"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCY0498899.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            6183..6662
                     /locus_tag="O0394_000800"
     CDS             6183..6662
                     /locus_tag="O0394_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HCY0498900.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(6990..7634)
                     /locus_tag="O0394_000801"
     CDS             complement(6990..7634)
                     /locus_tag="O0394_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCY0498901.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(7647..14822)
                     /locus_tag="O0394_000802"
     CDS             complement(7647..14822)
                     /locus_tag="O0394_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HCY0498902.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKVKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHINRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYFPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     VTPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIAVEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPETIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSE
                     LNVAQLRQAVQRLIARHEILRTQYIVVDDEVRQRIVADVAVDFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPNKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHHCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQVEQDLKQYLNDQLPKYMIPKT
                     ITHIDCMPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMYQNQQSLVALP
                     DNLSELQKIVMSRYNLGILEDSLSHRPLGNTLLTGATGFLGAYLIEVLQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEIMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTNPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSMAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMN
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            complement(15269..16519)
                     /locus_tag="O0394_000803"
     CDS             complement(15269..16519)
                     /locus_tag="O0394_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0498903.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGVFLTSLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            complement(16905..17930)
                     /locus_tag="O0394_000804"
     CDS             complement(16905..17930)
                     /locus_tag="O0394_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HCY0498904.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKALGLKTFLEERGHEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(18103..18462)
                     /locus_tag="O0394_000805"
     CDS             complement(18103..18462)
                     /locus_tag="O0394_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCY0498905.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            complement(18679..19710)
                     /locus_tag="O0394_000806"
     CDS             complement(18679..19710)
                     /locus_tag="O0394_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCY0498906.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNVQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
     gene            complement(19723..20484)
                     /locus_tag="O0394_000807"
     CDS             complement(19723..20484)
                     /locus_tag="O0394_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0498907.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWSFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            complement(20481..21455)
                     /locus_tag="O0394_000808"
     CDS             complement(20481..21455)
                     /locus_tag="O0394_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0498908.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKERSKNAQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEELRKQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            complement(21469..22209)
                     /locus_tag="O0394_000809"
     CDS             complement(21469..22209)
                     /locus_tag="O0394_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0498909.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGLQQKISDE
                     EINAQLKLVDLEDRGKHFPEQLSGGMKQRVALCRAHVHKPNVISMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPEYYL"
     gene            complement(22551..23063)
                     /locus_tag="O0394_000810"
     CDS             complement(22551..23063)
                     /locus_tag="O0394_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HCY0498910.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            23242..23445
                     /locus_tag="O0394_000811"
     CDS             23242..23445
                     /locus_tag="O0394_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498911.1"
                     /translation="MDKESVVASLARNKKIAVETMAGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDSIKEIDENHLNDAT"
     gene            complement(23507..24466)
                     /locus_tag="O0394_000812"
     CDS             complement(23507..24466)
                     /locus_tag="O0394_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCY0498912.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(25112..26599)
                     /locus_tag="O0394_000813"
     CDS             complement(25112..26599)
                     /locus_tag="O0394_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCY0498913.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGNPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            27026..>27138
                     /locus_tag="O0394_000814"
     CDS             27026..>27138
                     /locus_tag="O0394_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCY0498914.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFC"
BASE COUNT         8758 a         5153 c         4117 g         9110 t
ORIGIN      
        1 ggagaacaaa tacatatatg aagaagaaac taacatttaa agaaaacatg tttataggtt
       61 ctatgttatt tggtttattc tttggtgccg gcaatcttat cttcccaata cacttgggtc
      121 aagctgctgg ttctaacgtt tttatcgcta acttaggatt tttaattaca gcaattggct
      181 taccatttct aggtatcatt gctattggca tttcaaagac atctggttta tttgaaattg
      241 catcgcgtgt taataaaaca tatgcttaca ttttcacgat tgccttatat ctagttatcg
      301 gaccattttt cgccttacct agactggcaa cgacatcatt tgaaattgca ttttcgccat
      361 ttttatcacc aaagcaaatc actttatatt tatttatttt tagcttcgtc ttctttgtga
      421 ttgcatggtt ttttgcgaga aagccatcaa gaattttaga atatatcggt aaatttttaa
      481 atccggtatt cttagtatta ttagcaatta ttttattatt tgcttttatc catccattag
      541 gtggcatatc tgatgcacct attagtaaac aatatcaatc acatgcctta tttaacggct
      601 ttttagatgg atacaatacc ttagatgcgc tagcgtcatt ggcatttggt attatcattg
      661 ttgcaacgat taaaaagtta ggtatcgaaa atccaactga tatcgctaaa gaaacaatta
      721 agtctggtac tatcagtatc attatgatgg ggatcattta taccctacta gcaatcatgg
      781 gtacattaag tattggtcat ttcaaactta gtgaaaatgg tggtattgcc ttagcgcaaa
      841 ttactcaata ctacttaggt aactacggta tcgtcctgtt gtcacttatc gttatggttg
      901 cttgtttaaa aacagccatc ggtttgatta cggcattttc agaaacattc gaacaccttt
      961 tccctaaaat gaattaccta gcgattgcaa cagttgtaag ctttatttcg ttcttattcg
     1021 cgaatgttgg tttaactaag attattatgt actcagtccc agtgttaatg ttcttatatc
     1081 cattagcaat tgccttgatt gtactaacat tatttagtag caaattccat cattcaaaac
     1141 ttatttatca atgtaccatt ttctttacaa tgattgctgc attagtagat ggattaaaag
     1201 ctagtccaga gttcatttca agcacatcat tctcacaaac tttgattaat ttcagccaaa
     1261 aatatttacc attatcagac attggtatgg gctgggttgt tctcagtttg attggtttca
     1321 ttatcggctt cattatttat aaaattaagc atcgtaaaat tccacaagca taatactatg
     1381 ccacagtcat atgttaaaca tatgcttgtg gcatttttta ttcatactac attaaactgc
     1441 aatcgtatac atacatatca atgattatcc acaaaaaata ttagtacttt cattttacaa
     1501 atcacattaa tacaaacaca accttatctt tatattatta aatttacatt tgacacttat
     1561 attgaacaac tgtaatatat taatattaat tctttaaaat gtataaatat aaaggaggga
     1621 gaccgatgaa ttcaatcatt gaattaactg attattatag ctctaataat tatgcaccac
     1681 ttaagcttgt catttctaaa ggtaaaggtg tcaaagtttg ggatactgat ggcaaacaat
     1741 atatagattg catttcgggt ttttcagttg caaaccaagg ccattgtcat ccaacaattg
     1801 ttaaagcgat gacagaacaa gcttcaaagt tgtctatcat ttcacgtgtc ctttatagtg
     1861 acaatctcgg gaaatgggaa gaaaaaattt gtcatcttgc taagaaagac aaagtactcc
     1921 cccttaactc tggtactgaa gctgttgaag cagccattaa aattgctaga aaatggggct
     1981 ctgaagttaa aggcattact gacggacaag ttgaaatcat cgctatgaat aacaattttc
     2041 acggtcgtac acttggctca ttatcactat ctaaccacga cgcatataaa gcaggatttc
     2101 accccctact tcaaggcact acaacagtag attttggaga cattgaacaa ttaacacaag
     2161 ctatttcacc gaatacagca gcaattattt tggaaccaat tcaaggtgaa ggtggcgtta
     2221 atataccacc gaaaggatat attcaagctg tgcgtcaact atgtgataaa catcaaatat
     2281 tattgattgc agatgaaatt caagttggtc ttggtagaac tgggaaatgg tttgctatgg
     2341 aatgggagca agtcgttcca gacatttata ttttaggtaa ggcattgggt ggcggcttat
     2401 accctgtatc tgctgtactt gcaaataatg atgtcatgcg tgttctaaca ccaggtacac
     2461 atggttcaac atttggtggt aaccctttag ccattgcaat atcgacggca gcgcttgatg
     2521 tacttaaaga tgaacaactg gttgaacgat cagaacgctt aggttcattt ttattaaaag
     2581 cgttgctaca acttaaacat cctagtatta aagaaattag aggtcgtggt ttatttatag
     2641 gcatagagct taacacagat gctgcacctt ttgtggatca actgattcaa cgtggaatct
     2701 tatgcaaaga cacgcatcgt actatcattc gattgtctcc acctctagtc attgataaag
     2761 aggaaatcca tcaaattgtt gcagcttttc aagacgtttt taaaaattaa caattaatca
     2821 tttatatatg acataggagg gattcatgat gattaaagta ggtatcgttg gcggtagcgg
     2881 ttatggcgca attgaattaa ttcgattgtt acaaacacat cctcatgtaa cgattgcaca
     2941 catctactca cattcaaaag tagatgaacc gttgaaatta acatttccac atttacaaca
     3001 tattatgcaa catttcgaag cacttacagt ggacaataat gactgtgatg taattttctt
     3061 tgcgacacca gcacccgtaa gtaaaacatg tatccctccc ttagtagaaa aaggtattca
     3121 tgttatcgat ttatctggcg catttagaat taagaatcgt gaaatatatg aagcatatta
     3181 caaagaaact gctgcagcac aagatgattt gaatcatgct atttacagca tttcagaatg
     3241 gcaatcgttt gataacaatg gaacgaagct catttctaat cctggctgtt tccctacagc
     3301 aacattatta gcattacatc cacttattag cgaaaaaata gtagatttgt catctattat
     3361 tattgatgct aagaccggcg tgtcaggtgc tggtcgttca ttatcacaac gtgttcattt
     3421 ttcagaaatg aatgaaaatc taagcgctta tgcaatcgga aaccataaac acaaaccgga
     3481 aatcgagcaa tatttatcta tcattgcggg tcaagatgta tcagtcatat ttacaccaca
     3541 tctcgtacca atgacacgag gtattttatc aacaatatat gtcaaattat catctgaata
     3601 tacgactgaa tcattacata aattaatgac ctcttattat gctaatcagc catttgtcag
     3661 aattagagat attgggactt ttccaaccac aaaagaagta ctcggtagta actactgcga
     3721 tatcggcatc tatgtagatg aaacaacgca aacagcaatt ttagtatcag tgattgataa
     3781 ccttgtcaaa ggcgcaagtg ggcaagccat tcaaaattta aatatattat atgattttga
     3841 agtgacgact ggcctaaatc aatcaccagt ttatccataa ggggtgttag aatgaaacat
     3901 caagaaacga catcacaaca atataacttt tcaattatta aacatggcga tatcagtaca
     3961 cctcaaggct tcacggctgg tggtatgcac atcggtttac gcgctaacaa aaaagacttt
     4021 ggatggattt actcatcgtc tttggcaagt gcagctgccg tatatacttt aaatcagttt
     4081 aaagctgcac cacttattgt cactgaagac actttacaaa agtctaaagg aaaattacaa
     4141 gcacttgttg ttaattcagc taatgcaaat tcttgtaccg gtcaacaagg catagatgat
     4201 gcacgacaaa cacaaacatg ggttgctcaa caacttcaaa taccatctga gcatgttgct
     4261 gttgcttcaa ctggggtcat tggtgaatat ttgcctatgg ataaaattaa gactgggacc
     4321 gaacatatta aggatgctaa ttttgcaacg ccaggtgcgt ttaacgaggc aattttaaca
     4381 actgatacct gtacaaaaca tatcgctgta tcactaaaaa tcgatggtaa aaccgttaca
     4441 attggtggta gcaccaaagg ttcaggtatg attcacccaa atatggctac catgcttgct
     4501 tttataacaa ccgatgcatc gattgaatcg aatacacttc atcaattatt aaaatcttcg
     4561 actgaccata catttaatat gattactgtt gatggcgata caagtacaaa tgacatggta
     4621 ttagtcatgg caaatcacca agttgaacac caaatactta gtcaagacca tccacaatgg
     4681 gaaacatttg ttgatgcatt caattttgtc tgtacatttt tagctaaagc tatagccaga
     4741 gatggcgaag gcgcaacaaa gttaatcagc gtcaatgtgt caggcgctaa atcaatatca
     4801 gatgcaagaa aaatcggtaa aactatcgta agttcaaatc tagtaaaatc agctattttt
     4861 ggcgaagatg ccaattttgg tcgaatcatt acagctattg gctacagcgg atgtgaaatt
     4921 gatcctaact gcacatatgt tcaactgaac caaatacctg tcgttgataa aggtatggct
     4981 gtactatttg atgagcaagc tatgtcgaat acattaactc atgaaaatgt cacaattgac
     5041 gttcagcttg gtttaggtaa cgctgcagcg actgcatacg gttgtgattt atcctatgat
     5101 tatgtgcgta tcaacgcatc atatcgaaca taaggtggtg ttggttagat gaaatttatt
     5161 gtcattaaaa ttggtggcag tacacttagt gacatgcatc catcaattat taacaacatt
     5221 aagcatttac gatcaaacaa catctacccc attatcgttc atggcggtgg cccatttatt
     5281 aatgaagcat tatcaaacca gcaaatcgag ccacactttg ttaatggcct aagagtgact
     5341 gataaagcaa ccatgaccat tactaaacac acgctcattg cagacgttaa cactgcatta
     5401 gtagctcaat ttaaccagca ccaatgttct gcaataggct tatgtggttt ggatgcacag
     5461 ctgtttgaaa ttacatcttt tgatcaacaa tatggatatg tcggtgttcc gaccgcttta
     5521 aataaggatg ctttacagta tttatgtact aaatttgtac ctatcatcaa ttcgattggt
     5581 ttcaataacc atgatggaga attttacaat attaatgctg acacgcttgc ctattttatt
     5641 gcatcatcat taaaagcgcc tatttatgta ttaagtaata ttgcaggtgt actcatcaat
     5701 gatgttgtta tacctcaatt gccattagtc gatattcatc aatatattga acatggtgat
     5761 atttatggag gtatgattcc caaagtgcta gatgccaaaa atgcgattga aaatggctgt
     5821 cctaaagtta tcattgcatc aggaaacaag ccaaatatca ttgaatctat ttacaataat
     5881 gattttgttg gcacaacaat ccttaattca taactatgaa attaaggcct aacaagtttt
     5941 gacacgcgag atgattccag ttcgattatc cattgcgcta aaacatttat ttaccgttca
     6001 tctcgttaac aattttgaat acagtacgat acaatatgag atgtaaaaaa ctaataacct
     6061 tttacaaatt tgtttatcaa aatattttaa gttttgcaaa gctttttatt gtgattattt
     6121 tcacaaaata ctataatgag gatagtaaat agagaggagt ccttaagttg acgaaacgac
     6181 aaatgggtat attcatttat gctggaatta tcggtggctt gttatctgga attgtaaaat
     6241 taggttggga ggtcatgttt ccacctcgca caccagaacg taatgcaacg aacccacctc
     6301 aagagttatt gcaacaatta ggatttagta gtgagtttac gcatcaaaca tatacatttt
     6361 caaatatgga attgccttgg gtaagcttta ttgtccactt tagtttttct atcgtcattg
     6421 caattattta ctgcatatta gttaaaaaat acgcttactt agcaatggga caaggtgctg
     6481 tttttggtat tgctatttgg gtattattcc accttatcat tatgccaatc atgcatactg
     6541 tacctgctgt gtgggatcaa ccattccaag agcatctgtc agaattcttt ggccatatcg
     6601 tctggatgat gacaattgaa ttagtgcgac aacattttgt ctatcgctat aaattaaatt
     6661 aatacactga ctaacattaa cgtgagtttt aaatcatcgt ttgagtatga tgattgatgc
     6721 tcacgttatt ttattaactg acatgatatg attccagcca acttacgtga gcattaaagt
     6781 ctcaaatgcg tcgtaacaaa ctattatttt cggtaatttc aatattgctc agtatatttt
     6841 taccttatca cttactttaa tctcgtcatg attttgaatg atgccatcgt gtattcacct
     6901 ttcatttttc caataaaaaa acatctaaca gtaaacattt aggcagtata gtttaaaatc
     6961 actgcgcaat gatactgtca gacgtcatat taactactca ataactgaaa tacagacact
     7021 tttttataac cccagggtgc ctgtcctaag aaacatacct gtaccataaa ctgatcaaaa
     7081 ataaattgtt tgaacttcac ttcacgtgat tgataaaagt gtgattgtgt catatcataa
     7141 atgtccaatc ctttgattaa accttcacca atcaattttg taaaactttc tttttgtgtc
     7201 catatttgat aaaaatcatt taaactacat atttgatgtg cttcgtttgt agagaaacac
     7261 gtcactaacg tacgccagtc taaacgttgt gatatctttt cgatatcaat accaactggt
     7321 tctttatcga caacacacac gatataagga taactatatg ataagctcac atagatgggc
     7381 tgtccatcac gattgtgttg aacaatatct gccttacctc gtggcgaaat gtgataatgc
     7441 cattcatgtg gtaataaacc tgtgtcatgt tgaattccat attgcactaa aatatctccc
     7501 aatctgtgca tgagtttatc ttgattgtat ctatagttga ctgtacgcgg ttttttatat
     7561 gaccaacgac tttgtgatat taattcttca atacttttca agttactctg taattgcatt
     7621 acaaatactg tcataacttt ccctacttac ttattgaata ttgttttgat atattgtgcc
     7681 caatgataca gccaattgtt agttatcgtt ggccattttt cactgatgtg attcattatt
     7741 tttaatgtta atgttgtatc tatcattgct agttgttgtt cacggtcaac actagttaat
     7801 ccaatcgttt cgtacatgtc ttgtttctgt aaaatttcat taaatgattc atcgctgacg
     7861 agttcaattt ctttgcgctt aacgcattct aacaaagatt tcaccggcat tttattaggt
     7921 gatagcacat ggtaaatgat ttgtggtgtg ttgacctgtg ctaatgcgac aatttgtctt
     7981 gcagtcgtat ccacaaaaga aaaatctaca ggcatttcag ccatgctaac cccgatacaa
     8041 tccagttgta acaaatcatt cattaccatt gaaaaacggt tagtctttat atttctcata
     8101 tgccatcttc cattgtaagg attcgtcaaa ttaccaacac gtacaatccg accatctaag
     8161 ccattattta cagcttctaa tacttttaat tcactataaa atttgctccg tgtatatggt
     8221 gatgttagta gttgcccttt atagacatcc gcttctgaaa atgtcacatc ttctgtgtct
     8281 atatcaaaat aagttcccac acttatcgta gacacatata ttaaccttgc atgatgttgt
     8341 tgtgccaaac gtatgacatc aacagtacct tgaacattta ctttttcaaa ttcatcatca
     8401 tcaccaaagt gatctgtacg agcacctgca tgaataatcg tatccatgtt ttctggtaaa
     8461 acaacatcat ccatacactc gaaatcacca acaatgactt caatgtttga taacattatt
     8521 tcaaccgtct cttctgaaaa ataatcattt aaattcgtca tcaacttata ccatgctatt
     8581 tcctcattat cagcacgtat gaaacaataa atgcgatgac tgtatccttg tagtacttca
     8641 atcagataag cacctaaaaa acctgtcgcg ccagtcaata gtgtatttcc tagaggtcga
     8701 tgacttagac tatcctctaa aatacccaag ttataacgag acataacaat cttttgtaat
     8761 tccgaaagat tatccggtaa tgcaactaat gattgttgat tttggtacat ataattaaca
     8821 atctgtcgca cggttttata ttggtataat gtctgcattg aaatatgatg gccaaatcgt
     8881 tttaaatgcg agacaactaa catcgcctct aatgagttac caccaagttc aaagaaatca
     8941 tcgtcaacac cgacatcatt ttgtttcaat acctctccaa atacatcaac aaatgtctgc
     9001 tcaatttcat tagagggttc gctatacact ttattagact gttgtatagg tgatggattt
     9061 ggcaaacgcg tagtatccac cttgtcattc gtggttaatg gcatacaatc gatatgcgtt
     9121 atagtcttag gaatcatata cttaggcagc tgatcattta aatattgctt caaatcctgt
     9181 tccacttgtt gctctccgac ataataagca ttcaatatat catgcgtatc aaagtgactt
     9241 actgttacaa cacaatcaga tataccacga atagctaata ttgcattttc aatttcatca
     9301 agttcaatac ggtacccgtt aactttcact tgtttatcta ttcttcctaa aaattcaatt
     9361 tgaccatcag atgtataacg tgctaaatca ccactatgat acaactttcc tttaccaaat
     9421 ggattatttt gccatttatc agccattaat tctggacgat taatatatcc tatcgctaaa
     9481 ctatcacctg caatacacaa ctcgcctggc ataccaatac cgcataacaa accatctgac
     9541 ataatataca cttggatgtt agataagggt ttgccaattg gaatcgtctc aggtatcaaa
     9601 tcaccacaat gatgtgacca atacgatgtg atgactgttg actcagatgg tccataggca
     9661 ttgaaatacg tgccacaatg cttctcaata tatttaacaa aggatgccgt actagttgcc
     9721 ccgcctgtaa tcaacttttc aatataaaag tcttccataa cactacacat ctgtaacgga
     9781 atcgacgcaa ccgtcacacg atgcttatta atgagttgtt gtaactgttc tggattaaca
     9841 cgttcctctc tatctggaat cacaagcgta tgaccattta acaaacaaca ataaatctcc
     9901 ataactgatg catcaaaaac aatatttgca tgttgcaaaa atacttcatt gtcgcctaat
     9961 tgcaattcag ttgaccatgc atgcactaaa ttcaacaaat ttcgttgtcg tatggcaacc
    10021 cctttaggca tcccggtcgt accagatgtg taaatagcat acatctcatt atctaacatc
    10081 gctgtgtttt caagttgatt gccatgtaaa tcatcatatt gttcattttc ctttgattca
    10141 acaaagcctt tagcattttc caattgaatg actggtaatg tcgtttcaat ttcaacgccg
    10201 taagacatga ctgcagttac tttagcatcc tccaaaattg caccttgtcg tttattcgga
    10261 aaatcaatat cgataggtat ataagatgca cctactttaa ctgtcgccaa catcgccgca
    10321 atcatttcaa aactacgttc tgtaaacaag gcaacccgtt gaccattgcc cacaccattt
    10381 gataggagca tgtgcgcaat ggcatccaca tagttgcgta atgtttcata cgtcattgtc
    10441 aaatcattca tgactagcgc aacatgatta ccttgtcgtg agacaacttc attaaagtaa
    10501 cttatgatag atttatttcc cgggacatta agcattcgat cgttaacatg cgtattgacc
    10561 caatttagaa gttcctccgt gccgtttggt atatcacaaa tttgtagtgt atcttgatgc
    10621 ttcaaaatat aatcaatcat aatcatacat tgattaccca tgtgacgaac tgtttctgag
    10681 tgatataaat cggtattata ctcgatattg attgtatagt catcgcgatc ttcttcaatg
    10741 atgaaagata aatcaaattt cgccgtcact gatttgggtt gaatgtgtgt taatttacta
    10801 tgcccaaaat gagcatgatt cgtttcattg ttttgtagta ctaacatgac atcaaataat
    10861 ggattccgtg aggcatcatg tgattgatct aagtcattta ctaaacattc gaatgggtat
    10921 tcttgatgct cgtatgcctc caaactcatt tccttaacct cttgtaaaaa ctgtgtccac
    10981 attttatcag gtgacggttg ccctctatat accaacgtat tagcaaacat gcctagcatt
    11041 tgctccgtgc ctttatgcat acgcgcactc atcacactac cgacaacaac atcatctttt
    11101 cgagcatatc tacttaacaa cgtcatgacc acactcataa agaacataaa atcagtaatt
    11161 tgatgctttt ctacatactt ttgaagtagc tgtctcattt gttgattcat tgtaaatgac
    11221 atcattgctc catttgtcgt tttaatattt ggtctaacat agtctgtcgg taagcttaaa
    11281 ataggtactt catctttgaa ttgagataac caatattgtc tatgtttcgt catatcacga
    11341 tgcgacatcc actcactata gtctttatat tgcaatttaa gtggtaacaa taatttatgt
    11401 tgataaagtg cgttaagatc attcattaat tgtatattac tcataccgtc attaatgata
    11461 tgatgcgtat ctataaagag gtatgcatgt aagggacttc taatgtatct cactctaatt
    11521 tgacttggct tttccaaatt aaaaggtgct acaaattggc gcatgatttc ttgttcatcc
    11581 gtaaaatgcg tgttaacttc ttcaaagtca actgcaacat ctgccacaat acgttgtcga
    11641 acctcatcat ctacaacaat atattgtgtt cgtaaaatct catgtcgcgc tatcaaacgc
    11701 tgcactgctt gtcgcaattg agctacatta agttctgatg ataaccgcca taaaaaaggt
    11761 acgttataca ccgtatcttt atggtttgat ttccataata aatacatacg cttttgtgca
    11821 gagctcagca cataatcatc tttaactata gtttctggaa tcacttcata gttttgttct
    11881 tgaaccttag caatcgcttg tgctagttca aatacagttg gcttttgtaa taaatcacca
    11941 atttgtaatc gtttcccagt agatgcctct atccgattca ccactaacgt tgcttttaat
    12001 gaatggccac ctaattcaaa gaaattatca tgaataccta cttgattcac atgtaaaata
    12061 tctgcaaaaa tttggcatag caagtgttcg gtatctgtac tcggtgctac ataggcatcc
    12121 gtatcgacat agtccatgat aggcaatgcc ttcttatcta atttcccatt aatagtaata
    12181 ggaatttgct caatatgcat gaaattaact ggtatcatgt actccggtaa ggtcatacgt
    12241 aattgtgatt taatcttatt atgtgataat gtatgcatcg cttcataata agcaacgata
    12301 tactgatctt gatcatgatt ttgaacaata acaactgctt tattaatacc ttgtatacgc
    12361 tcgagcgcat gctcaacctc tgacaactca atcctaaacc ctcgaatctt aacttgtttg
    12421 tcctttcgat ataaataatc tatgttgcca tcgggtaaca aacgaacgat atcaccactt
    12481 ctatacatca gctgatttat atttgaatct ttgataaatt tatctgctgt caattctggc
    12541 tgatttaaat aacctgcagc taacccaaag ccacttgtac ataattctcc aggaatacca
    12601 acgccacacc gacgctcgcc ttgcatgata taaacatgag tacccagaat cggtttacca
    12661 ataggaatac gatttggaac tttgttaggt atattatacg tcgttgtaaa tgttgtattt
    12721 tcagttggtc cataaccatt aataatttga ggatgcttcg gtttttgatt aagcaaatcc
    12781 acccacttag cattcaatac ttctccacca attaataaat actttaacgg taccaatact
    12841 tctattcgtt cactagcaat ctgattaaat aatgaggagg ttaaccacat agtattaacg
    12901 tcattttcat tgattaattg ttctaccgct attggattta ataattgttc ttttttagca
    12961 acaatcagct ttccaccatt gagcaatgca ccatatattt caaatgttgc agcatcaaag
    13021 gctatagttc ctgataacaa aatcgtcgtc tcttcattta atggtacata atgattttga
    13081 tggaccaatc gaacaatacc tcggtgcgga attagtgtcc ctttagggtt accagttgtc
    13141 cccgacgtgt aaataacata agcatgatct tctaacgtgt tacatttaga aagattatca
    13201 atatttttcc acgctatctt attcaaatca atgtgattaa tattttgttt accattttca
    13261 tataaagctt ggtacgttat tacaacttta ggcgttacat cttttaaaat gtactcctga
    13321 cgatcacttg gatagttcgg atcaattggc acgtaagccc caccagcttt caacacacct
    13381 atcatcgcta ttatcatctc aatacttttt tcagctatga cagcgacacg atcattaggt
    13441 tcaacaccat actggtttct caaacggtgt gctaaatcat tcgcgcgtgc attcaatgtt
    13501 tgatatgtta taaacactcc gtcaaattgc acagcgacat gattcggcgt tgcttcaact
    13561 tgttgctcaa ataaggtaac aactgtttgc gcatcatcta tctcaggcaa acttaaattg
    13621 atatcgtcat ataattgaat atcacgttct gtcatcaaat taagttcatc tacagttgtt
    13681 cgtttatttc cattttcttc agtaatttgc aaataaatat ttcgaactaa gtcactcagc
    13741 gtctcgattg agagcaaatc atatgctgca ctgttatata taattttgaa cccgtgttga
    13801 tgtggaaaaa tttcaatatc cgctaaagat gtatgtgcat catgtatttg atgtacatcc
    13861 tcaataacat cattacaaca agacatcata tgatgataac aatgaaaaat agtctctagt
    13921 gaaagcgaag acttcgcgca ctgtaattgc gacatatttt gcaacacaca tttattaaaa
    13981 tctgttgtaa aacgttgaca tacatctttt gcatcgattg ttaacgttaa cggcacaata
    14041 tttccgtgta aatcatttgg taaatgtgat ggtacatgta tacctaatgt gacatcatgt
    14101 tgttgactca ttatatgatt agctaaatac acactaacag ccaacgatgc catatctatt
    14161 gatgtcatat catcaatcaa atacgtttga tataaagctt gttcaaatgg atgcttaatt
    14221 ggaaaataac tatcaatatg gatgtcagag ttattctcta accgaaaata gtttgagtct
    14281 aatgctatat gcgatgcatc ttgattgtct ttatcatcat ttctatttat atgtgcatgc
    14341 tgtcgagtat tgttaataac agtattgcca cgatatgcat tgcataaatc atcaagaaaa
    14401 atatcaattt gactatcatc gaaaatggac acatgaaaat ctaatagtat atatgcagca
    14461 tcagcgaact gaaacaattt aactttgaat aaaggtgaat cattaaaatg gtaagtactt
    14521 aattcttgct taaaaaaagc ttctaaatca tagtttgcgg aagaagatgg aacttgtttt
    14581 atctcaataa aaggcagaaa ttcatgaagt atcattgtta aattgtcatc ggtagtaaca
    14641 tcaaaaaaat gtcttataga tgcatgttgt tgcacaattg tcgataatgc atacatcatt
    14701 ttagtagctt caacattttt agcgagttta acccaatacg cattacggtg tgtcgttgat
    14761 tctgtattat ttttgtatat acgaaaatat tcctgttgaa atctcaaatt acccataatc
    14821 ataaaaagtc cttctttcat atcataatac tcattactta ctgaaattgc atgatgatat
    14881 gataaccgac gaaatgttaa ttaactcgtt atgtaatgat taatataaaa caccattcgc
    14941 aacatatgag cgatatattc taccctaaaa tacatcttgt atcatcgtta caattggtat
    15001 atttttcaat gtaaattaca tacatcttcg ataaatagca cactacaact cgttaatcac
    15061 tttctgttgt tcacatctca ttgcaaactc aatattgttg ttacaaaata tccatgaagc
    15121 aagtttatat taaacaaaca actcgcataa aacaattgtt atccttaaat tttaacaaat
    15181 tcttaataaa tttatctcta ttttaattgc gaccaaatta ataggttttc catataaaaa
    15241 gatgcataaa ataaatattt aaataaattc aatttgtatt cacttgtttt tgtcccccaa
    15301 atacaccagc aacaagcatg ctagcaccaa ttgttaaaac gataaacata tacagtccca
    15361 tttgtaatga cgttaagaaa acacccaaca caatccctaa cctagctagt gtttctgaaa
    15421 aatgaatacc taatgcatta actgcactat atgttcctct tttagcttta ggaataattt
    15481 taaagcgttg ttctgaaact ataggcgaat aaataatttc acctacagtc gcaattatca
    15541 taaaaacaac taataagcca aactgattaa gataggttag accactatag ccaacaatat
    15601 aaatcagcaa accagtgatt aaagcttttt taaaatctat tttcaataca actttcgaga
    15661 ttgaatacgt gagtaaaatg acgacgaccg tattaatcat tagcaagatt gctaacatct
    15721 tagcacctgt aatatcatat gaacctatac ttattgtttc aaactgatcc tttagtctaa
    15781 tagcaatata tgaggagatt gaaaattcac ccatcatgat gatactgaac cccgaaatca
    15841 ataacatata attacggtct ttcaaaacta atttataact gcgaaatata ttcattattt
    15901 gtaatttttg ataacgactt gcatgcctct tgtcatcact ttgctttact tgatttcggt
    15961 cttgaggtaa ccaaatatat aaaataaaga gtacaattaa aaatatacaa gctgctatta
    16021 agaaaagtag taacatactg tagccataca tcaagccacc taacaatgcc ccaatagcta
    16081 ccgataagtt tgtcatccaa tagctaatct tgtaaatata atgttccacg tcttcggtaa
    16141 ttgcatccat aattaatgtg tccataactg gaaattgtaa tccccaaacg attgtaaata
    16201 tggcatatgc aacacaaaaa ccaataattt gccacaattg atgtgaccca aatacgccca
    16261 tgaacacaag cattatcacc atcgtcgctt gataaataag tactagcaac tttttcggaa
    16321 atatctcaat aaggtaacca gatataatgg acaatggaaa ttttagaacc actaaaccaa
    16381 caagatatat accgacaatt gattgactta acatatctgt taaatatagt gctataaacg
    16441 gtataaatgc tgtcgtaata attagctgta aaaaattgct aatcaatcgt actttcaaag
    16501 tcgtacttaa gcgtttcatt aataactaca cccctttcca catatataca cactaccatt
    16561 tatataaaat ctattattag attatacgtt gaaacaaaga atttttagtt atacaaagtt
    16621 atttaacaaa caaatgcatc gactttacaa tgcatcttgc atataatgcc tcaaaaaaat
    16681 tagcactata tgactatata ttattccaaa tgaatgaaat ttatcaataa ttgcgtaagt
    16741 gaacacatat gtgcgtaaat gttaaatcat tgtcctaaca tagctgtgac tatacttaga
    16801 tgtaatcata aaaaaactac tgccatcttt acaacagcag tagtgcttat atgattcatt
    16861 tatataaaat tgaaagcgtt aatcgctagc ccagcatcct tattctattt agctgtataa
    16921 cttttactag caatacgacc acttgcaaca ataatatctt tatcttggaa aggttcatga
    16981 ttgaagaaac gctctaaaat atctttaact ccatcttcaa tacgtttttg tgcttctaaa
    17041 gtcatacctg aatagtgaac cgtcatagca tttctaggca ttgttctcca tggatgatca
    17101 gcaggtgcag gttgtggata ccaaacatca ccagcatatc cttgtaaatg ctcggatgct
    17161 aacgcttcaa ctaacgcatc gcgatttaca attttaccac gtgcagtatt cactaaataa
    17221 ctgtgttttt tcatacgact taaaacatct ttatcaaata agttatcagt ttctggtgtt
    17281 aatggtgcat gaattgtaat cgcatcactt gttgaaacaa gttcatcaaa gcttacaaat
    17341 ttagacaatt tatggtcttg ttgattgatt ggatcatagt gttgtaatgt tacattaaat
    17401 ggcgctaatc tttcagcaac aagttgtcca attcgaccaa atccaaaaat accaattgtt
    17461 ttgtgttgta attcatgcgc atgattacct acttgagaca agttccattc accttctact
    17521 gattgacgat gaccttcttc atagtttcta agaagtatta ataaatccat aaccgcatgt
    17581 tctgccacac taactgtatt acttcctgta acttcaacga caccaatatt gtgttcactt
    17641 gctgccgcta aatctacatg gtcagatcct acacctgctg taattgctaa tttcaagttc
    17701 ggtgcttttt caatacgttc acgagtcata tatgcaggat aaaatggcgc actaataatc
    17761 acatccatat ctggtaaatg tttatctaag tcttcaccat tatctgctaa tataatgaac
    17821 tcatgtcctc tttcctctaa aaatgttttt aatcctaatg cttttttagt attaagtaat
    17881 tgattttctt gaccttctac tgcttctgga aataatgcta cgattttcat atccatctct
    17941 cctaaatgat aaattttgac ttccttaaag tacgtgttgc atgttgcttg ttttaaaaat
    18001 atgacaaaaa aacggcaccg tttgacatac cacccccttg aattattgtt aaattcaaag
    18061 cgaagttagc aaacagcacc gtgtacctta caccagcaac acttattcta ttgacttatc
    18121 gaaaacaaat attgaaatga cttcatcatc attcaaatca atatctgtaa ataattttac
    18181 aaacttagcg cctacaagac tttccatctc atttacatct atttctttat acatctgttt
    18241 aatcttctct gtgcgtgtat aatggagcat cgattcattg cgtttgtcgt ttaggtaaaa
    18301 actctcaact ttactcaaaa cacctgtcat atgtgcaatc gcccaattat ctttaaacga
    18361 cactcgaata ctattgggtc ctttacccac gtattcttta cgataagatc ttactagatt
    18421 cgagaattgt tgttctttag tatgcgtatc tatagtattc accttcttta aaataagaag
    18481 ttcattaatt gttcacattt tcattataca ccttattaac actttttcaa tgataacgat
    18541 tattattatt acgaaatgta tattttccta gttaaaagga tataacaaat taacagaaaa
    18601 tgtttataaa ctaagattta tattatttta aaaaatgttt caacaaacgc atctaaacaa
    18661 aaaatcagct tatcacactt accccttcaa ctctgcttca agtttaccta aatgtcttaa
    18721 tgtcggtgtc aatgctgcga agaagaatcc ttcttttaac ttgcgaactt gtggcgaata
    18781 tggtgtgtac gctctagaac caccattgac aacagaagct tgatttacat ctaacaataa
    18841 atagccgatg tccttcttca atgatattaa ttcatttaaa tgtgaagtta agttaccgtc
    18901 atctaatatt gcataatatt cctctctaag ttgacgataa cgttttttaa aagcttcaac
    18961 atcatactct aaatattgat ttattccgtt ttgcacattt gaaaatgcat caattaactc
    19021 taaagaactt ttaattgagc ctaatcctat tggaatttgg taagcaataa attgcgggcg
    19081 aatagttgcc gcaaactgct tcgcatcatg cgtgataatt tgtgattgtg gcactacgac
    19141 ttgattcaat gtgatttgat acgttgctga cccgttgact cctaaaaaat tggttttttc
    19201 aacaagagtg ataccatctt gattggcacg tagaatgaac atgacaaatt catctgatga
    19261 ttcatgtttc gaaatcgcac caaaataatg gtcttcttga atattactta cagctggcat
    19321 acgtccactg acaaccaatt gtccatcaac ataagtgtgt tcaaggttca acttttctaa
    19381 atcattaaat gacttcatcg gattagacaa tccggtagca cctaatattt ctccagataa
    19441 caattgctgt tgtaagtcat tatttaaatg tggctgcgtg gcattttcta aatacgttga
    19501 aaaagctaat tggcaccata aacaaaatcc tgttgtcaaa caagactgcg atacagcttc
    19561 gattacttca gcattttttc tcaatgccgc ctcaccgaaa taaccatcta caaataattg
    19621 ctgaataaat gttctcggat aatacgtccc ttcatcaact tctactaaat gaggatctaa
    19681 ttgttctttg ataagcgttt ctaaagtcat catcatctct ccttattcac caaatcttct
    19741 aagtataaac tgctcaatat aactaatgaa tcgatcaata ataaaaccaa ataatccgat
    19801 aaagaatatt gctgctaaaa catcttctaa gttcaacata tttcgtgcat caacgattaa
    19861 aaaacctaat cccgattgtg caccaatcat ttcaccagaa actaaaaata tccaacttgt
    19921 tcctaccgcc atatgtatcc cagccatgat ttgtttaaat gccccgggaa ataatatatt
    19981 gcgatacaat gaccacccag ttaaatttaa atttgctgct atttttaaat attgaggttc
    20041 aatgtctcta acgcctttaa tagtattgaa cacaattggg aaaaaagcag cgataaaaat
    20101 aatcgcaatc gctggcaaac taccaatacc aaaccataga acaacaaatg gtgcccatgc
    20161 tatcggagat atcggcctaa tcaattgaaa tagcggttcg atagcgttgt atagccaacg
    20221 attccttcca agcaagaagc ccaatggaat agcaaccaac aatgcgacaa caaagcccgc
    20281 tacaaatctc cataaactaa ttgctaaatg ttggaaaatt tctccagtaa caatgaaaga
    20341 ccatatactt tttcctacaa gagcaggacc cggtaacaat acaggttggt aatgcccaat
    20401 aataatgacc atttcccaaa tgcctaagaa aataataaat gtgataatag gtaatataaa
    20461 tttgttattt gtgggacgtg tcatgaacgc gatgcctcct tatacaatga cggttcaaca
    20521 aagtcatcat atgcaggtgg attaaacaaa tgatgttgtt ttaccaatgt cgtaatttct
    20581 tgatagccgg atggcttaat tgttaaatca ccataggatg tccatgccgc tgactgtgtt
    20641 aaaacgtcac gactttgttt aaaatgatgc gtcataatgt ctacactttg cttgcgatca
    20701 ttcattttaa agccagactt tttataatct tgtacaaatg cttgcgctac atccttgtgt
    20761 tgatcaagca attcccctct cagtactagc acacagcaat acgcatcagg tataacgtca
    20821 tcaccatgtt tcaaagtctt acctttgcct aacttttcac ccagtgcacc gaatggttcg
    20881 gctacagaat accctgtaat tctgtgttca ctcaatgcgg ctggcatttc tgctggcgac
    20941 atttcatgat agctaaaatg ccccggttta atctttaatt gtttacgtaa ttcctcaagt
    21001 aaaagataat gtgttgaata acgatgtggt ataccaaaat ggtaatcatc gccattatta
    21061 ttaaattcat ttaagtgcat acctttttgt cccataatga cattgccttc atgatggccc
    21121 aatgccacag cctttatatt tgagcccttc tgttttgatt tcatcgctag ctctattaaa
    21181 gttgatgcac catcaatacg accactgttt aatgcgtcca ttaaatctgg ccaattattg
    21241 aatttaacta attctagttt atatttcgga tgattgtatt gtgataataa ttttttagtc
    21301 atcatcaaat tagctgaatg tgtaatcggc aaatatccaa ttttaatcac ttgctgattt
    21361 tgggcatttt tagaccgttc tttagacgtc ctttgccaat cacatcctgt aattataaag
    21421 attccaatga tgacgattat gcttaacctt ttcatcgtca ctcactcctt ataaataata
    21481 ttcaggttca acttgatgat gattcaatgc aaatgtttcc ataatttcat tacgaatctt
    21541 aagtaggtgg ctatcattac gactgcgtgg atgtgatgct gtaatttcat attgagaaat
    21601 aatattgcac ccttcaccta acagaacaat gcggtcggaa agataaatag cttcatcaat
    21661 gtcatgcgtc actaaaataa tagttgattg cgttttatgt tttagttgca ctagttgatc
    21721 ctgaagttta taacgtgtaa atgcatctaa tgcacctaat ggctcatcca tcgatataac
    21781 gttaggctta tgcacatgcg ctcgacatag tgccacacgt tgtttcatac ccccggacag
    21841 ttgctcggga aaatgctttc ccctgtcttc taaatcaact aatttaagct gtgcgttaat
    21901 ctcttcatca ctaattttct gttgtaatcc aatcctaatg ttgtcattaa tcgttttcca
    21961 tggcagcaaa ttatgatgtt gaaatagcat taaacaatct ggagatggct gttgtttaat
    22021 ttcgttatca ataatgacac gaccagacga tggatgaata aatccaccga taatattgag
    22081 taaagtagac tttccgcaac cacttttccc tatgaaagtg actatttctc ccttgctaat
    22141 gtccaaatta aagttatgaa ttactttatg tgatccaaag tgatgttgta attgttgaat
    22201 tttaatcatg acaagccccc ttttaacccc tactatttta ataggaatta agtgtatatc
    22261 taaagggcta tgccaaacac agcccttata tcgtgaattg ttatatctat agatgattat
    22321 ggtttggtat gagtgctctt ataacataag cgttattcaa ttaacgacgt tatattaatt
    22381 tcagctctac taaatttcaa taaaatgatt gttttagaca atacttgcat ttagttcatt
    22441 agtttcttca tatcaactac ttacatttat agcattgagc tattctaatt aactgcaatc
    22501 gaaatccatg ccaaacctat atacatatat ctcatcaact tgttgtctta ttaaagtttt
    22561 tcgatgccat caatcggtgt atcaactgct ttgcgcgcgc gacgtaccgc ttcttcatca
    22621 ggtgcgttgt ataaacaaat acatttagac atatcttcac atacatatgt gcgtttaaat
    22681 tcaacttctg gcacttcttc ataatgaaca gaatttttct ttttacgtgc taaatattga
    22741 tccatcgtaa tgccttccgg aatgttccaa gttacaaggt aatcaacatg tgcatcacca
    22801 tttttaactt catctaaatc tttaccaact aagcgtactt ctttgactaa ttgaatacta
    22861 atatctgctt ctgttaaaaa ttgtttagct tctgcttcgt catttgcttc cacaataaaa
    22921 taaccatgag ttaaattttc agtagcttgt acttcaatta atgttggaat tgtcttcgta
    22981 gatagtgatg ctgcctttgc ttctagttct tctttcgttg atgcaaaatc aagattgtta
    23041 gcttctaata aaaataatgt cattaataag acctcctata acaatggttg aaaaaatatt
    23101 actataattc ttatcggatt acaaggatat taagatattt ttttaaatat ttagataaaa
    23161 ttctgtaaaa tatattttca aagagtataa aaatcatgtt agggtataag taaataaaca
    23221 tatatatagg gggctatcat tatggataaa gaatctgtcg tagcaagttt agctagaaac
    23281 aaaaagattg ctgtagaaac aatggccggt caaaggtaca tcatagaacg cattttacat
    23341 acaaatgatg aaaaacatat tcatattttg aaacctaaag atgtcgtgtt agatgtagat
    23401 agcatcaaag aaattgacga aaatcattta aatgatgcaa cataagtata aaacgaaacg
    23461 tgcgcctatg acatagatta atgttttagg cgctacaatg ttttatttat gttaatttgt
    23521 cgaagtatgg ctcatctaat ggattcaatt gcaagttttc aatttgcaac gttgaatgtg
    23581 caatgccata tttttctttc aacaatgatg atacttgatc aatcgcttga taatcatcac
    23641 cctcatattt tttatctaac acaacatggg cacttaatga ataatgctct gttgtaatac
    23701 tccacaaatg aaattcatgt acatctaata tgccatctat gtttttaata tctgccataa
    23761 tttgatcagt atccaaatgt tgaggcacac tttccattaa aattaaccac gcattacgcg
    23821 taattttata accaccacgt aaaatgatga gtgaaattac aatactaatg attgggtcga
    23881 tgatgcgcca tcctgtaaag taaatcaata caactgcaac gatgacacca atagagttca
    23941 ataagtctcc catgaaatgc cataatgcac tttgaatatt gatattgtct tcttgtttta
    24001 aagaccttac aaggataaca gtcaaaataa tattgacgag taaaccaata ctagcaatca
    24061 taaacataat gccactttca attggttgcg gataaataat acgtacaata gcttcatata
    24121 aaatccagat tgaaattaca attaatgcta aaccatttaa aaatgcagct aatatctcaa
    24181 atcttaaata tccaaatgtg tatcgtgcag tcggcttttt acttgcaaaa taaatggcca
    24241 acatagataa accaagtgct aatacatcac taagcatatg aaatgaatct gacagtaatg
    24301 ccaatgaatt agatactaaa cctccgacaa attcaatcac tgtaaataac aatgtgatga
    24361 ttagtgatgc ccacagcgtc tttttagaac tttgttgaat tttttgatga ttgacatgat
    24421 gaaaatattt gtatctgttt atcgtattcg cctttttccc attcatacgt ttgccactcc
    24481 taaataaatg cgtcgtattt aatattgtcg cttttaaaaa taattcttta tagcttctca
    24541 ggttaatatt aatgcttccg taattaacca tgatttatac atcatcgtca tttacatgac
    24601 aactcattaa tctttaccct ttatagcttt atgtaatatc accttggaaa acttttacag
    24661 cacactcgcg ctttgcttta ttcctatcat atagaaaatt ttaataatgt acgagattat
    24721 tgtgttagtt acataaaaat gacaaaaaag tatctcaaat ttattgttta gtttcattaa
    24781 gttcaaaaaa tcgcaataac tttaatgatt gttccgcata ttaaaacaaa cgatgacttt
    24841 gcatattgaa cttcatattt ctaaatcacc ataattaata aacaactctg tccttataat
    24901 agccgcatcg caaattagca taattaaaaa cagcagtaag atgatttgca attaaaaacc
    24961 atcctactgc tattatttta gtcattatat cctaatatca tgatacgtgt tcctatatat
    25021 tttgttttgc ttaaaatcta aacaacgccg ttaaatgtta agacttaccg ctagactgac
    25081 ctacaaacac gcttcagaaa cgatatttat tctagtacaa accttttaaa gcattgcttg
    25141 tatcaatata aatattttta acttgttgat agttacttaa cgcaccttta taagtttctc
    25201 gaccgatacc tgatttttta taaccaccaa atggtgcgcc ttctggtact tggttgtaag
    25261 tgttaatcca aatacgtcct gtacgtacag ctttagcaat atttaatgca cgtgtgatat
    25321 tttgagaaaa tacaccgcct gctaaaccat actcagaatc attagctata tcaattgctt
    25381 cttgatcgtc cttcacttta atcactgtta acactggtcc aaatatttct tcttgtgcta
    25441 atttgtgatg attgtctggc acagcaatta atgtcggctc aaagaagaac cctttatcta
    25501 atccattttc agttaagcga tgaccgcctg ctaaaatttg tgcatctgat tcttttgctg
    25561 catcaatata tgattgaatt ttatctaatt gatccttacc agtttgacta cccatttgtg
    25621 tagcttcatc ttgtggattt ccaactttaa tatttgaaaa tgcctcttgt aaacgtggca
    25681 ccaattgatc ataaattttt tcatgaacta ataatcgaga acctgcacta catacttcac
    25741 cttggttgaa taaaatacct aactgaatac cttcaactgc aaggtctaaa ttagcatcat
    25801 ctaatatgat attggcgctt ttaccaccaa gctctaatgt agcgggtact agatgttttg
    25861 ctgcagcttc ggcaacttga taacctacat cagttgagcc cgtaaatgat aatttatcta
    25921 caccatcatg attgaaaatt gcattacctg attctgaacc tttacccgtt agtatattga
    25981 caacaccttt aggtaatacc tcttggaaaa ttttagcaac ttccaataaa cttaatggtg
    26041 ttgaagacga aggttgaatc acaattgtat tacctgcagc aatggctggc gcaatcttcc
    26101 atgcagctaa tagcattggg aagttccaag caacaacagc acctacgacg ccaatcggct
    26161 catgtcgtac gatactcatt gtgtctttat cgatatcatt cactgtacct tcttctgttt
    26221 caataacact tgcgaaataa tggaaatgtc ttgcagcaaa tggaatatca attgctgttg
    26281 tctcacgaat cggtttacca ttatttaatg tttcaatcat tgcaatttta tctttttgtg
    26341 ccattaattt atcaccaata tcacgcaaca tttgtgcacg ttctgattta gaagttaatg
    26401 accatgattc aaatgcctct tgcgccactt tgactgcatg atcgacatct ttatcttttg
    26461 ctcttgtaat atgtgatagt gtttctccag ttgctggatt agtcacttcg attgtttcgt
    26521 cactgctacc tttaacaaat tccccattga taaataaacc ataattctct gcaatataat
    26581 ctcgaacgtt tactgccata ttatctctcc tttgtttatt ggctataata taaatctctt
    26641 agcatcatat ttaataatgc ttttaaacga tatgattcac attatatagt aagcgtttac
    26701 attttataaa ataatcagct cacagaagtc tcatttttaa aacttttgcg tcgtttttgc
    26761 acataaaaat aatcttattt tcgaaaacat attgcacttt tcaaaaataa aacacataat
    26821 ataactataa tacttgagaa taattctcat taaatagttt ggttgttatg aaagtcgcgc
    26881 ttaaaatgcg ttaatgtgac aaggataatt caacatcgtt tcattttact gagtcattgc
    26941 acttatcata cacattatat ttagcatgag ttatattact aaaacaatat tacatgcgca
    27001 ttagtactag aatggagttt tttcaatgcg acttatttta atcgtcattg gcttaatatt
    27061 tacagcgctt ggtattgcag gtgccgtatt acctttactg ccaacgaccc cttttttact
    27121 cgtagcagtt ttttgttt
//
LOCUS       DAKTLH010000022        26440 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.022, whole genome shotgun sequence.
ACCESSION   DAKTLH010000022 DAKTLH010000000
VERSION     DAKTLH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26440
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.022"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..525)
                     /locus_tag="O0394_000815"
     CDS             complement(<1..525)
                     /locus_tag="O0394_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HCY0498915.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQD
                     IKISNTPKNSSQGNNLVINNNELTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREA
                     PKTQIYTVKKGDTLS"
     gene            complement(752..1660)
                     /gene="sucD"
                     /locus_tag="O0394_000816"
     CDS             complement(752..1660)
                     /gene="sucD"
                     /locus_tag="O0394_000816"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCY0498916.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYESLLTVNK"
     gene            complement(1682..2848)
                     /gene="sucC"
                     /locus_tag="O0394_000817"
     CDS             complement(1682..2848)
                     /gene="sucC"
                     /locus_tag="O0394_000817"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCY0498917.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            complement(2957..3724)
                     /locus_tag="O0394_000818"
     CDS             complement(2957..3724)
                     /locus_tag="O0394_000818"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCY0498918.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            complement(3708..4592)
                     /gene="ylqF"
                     /locus_tag="O0394_000819"
     CDS             complement(3708..4592)
                     /gene="ylqF"
                     /locus_tag="O0394_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCY0498919.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            4993..7599
                     /gene="yfhO"
                     /locus_tag="O0394_000820"
     CDS             4993..7599
                     /gene="yfhO"
                     /locus_tag="O0394_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HCY0498920.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLRSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSANMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKLICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWILF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVCI
                     IAILYVLLSPTHPLALIVGIILLIVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSTLKQHDYHSNYVNQLIKKINQNATGSFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYGFKIKSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNIKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRVVTPVTIRIKAPDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKHGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITIFGII
                     CSIIFTRWARQK"
     gene            complement(7843..8193)
                     /gene="rplS"
                     /locus_tag="O0394_000821"
     CDS             complement(7843..8193)
                     /gene="rplS"
                     /locus_tag="O0394_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCY0498921.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(8296..9033)
                     /gene="trmD"
                     /locus_tag="O0394_000822"
     CDS             complement(8296..9033)
                     /gene="trmD"
                     /locus_tag="O0394_000822"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCY0498922.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            complement(9033..9536)
                     /gene="rimM"
                     /locus_tag="O0394_000823"
     CDS             complement(9033..9536)
                     /gene="rimM"
                     /locus_tag="O0394_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCY0498923.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            complement(9724..9999)
                     /gene="rpsP"
                     /locus_tag="O0394_000824"
     CDS             complement(9724..9999)
                     /gene="rpsP"
                     /locus_tag="O0394_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCY0498924.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            complement(10434..11801)
                     /gene="ffh"
                     /locus_tag="O0394_000825"
     CDS             complement(10434..11801)
                     /gene="ffh"
                     /locus_tag="O0394_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCY0498925.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTQLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            complement(11827..12159)
                     /locus_tag="O0394_000826"
     CDS             complement(11827..12159)
                     /locus_tag="O0394_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCY0498926.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            complement(12146..13396)
                     /gene="ftsY"
                     /locus_tag="O0394_000827"
     CDS             complement(12146..13396)
                     /gene="ftsY"
                     /locus_tag="O0394_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCY0498927.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSEGSTQDA
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGSK"
     gene            complement(13396..16962)
                     /gene="smc"
                     /locus_tag="O0394_000828"
     CDS             complement(13396..16962)
                     /gene="smc"
                     /locus_tag="O0394_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCY0498928.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLMELLE
                     NISNEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIYGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETYLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQNQQTKHQLKDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEAKLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            complement(17109..17840)
                     /locus_tag="O0394_000829"
     CDS             complement(17109..17840)
                     /locus_tag="O0394_000829"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCY0498929.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            complement(17956..18189)
                     /locus_tag="O0394_000830"
     CDS             complement(17956..18189)
                     /locus_tag="O0394_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCY0498930.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            complement(18432..19166)
                     /gene="fabG"
                     /locus_tag="O0394_000831"
     CDS             complement(18432..19166)
                     /gene="fabG"
                     /locus_tag="O0394_000831"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCY0498931.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            complement(19159..20085)
                     /gene="fabD"
                     /locus_tag="O0394_000832"
     CDS             complement(19159..20085)
                     /gene="fabD"
                     /locus_tag="O0394_000832"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCY0498932.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAANTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            complement(20078..21064)
                     /gene="plsX"
                     /locus_tag="O0394_000833"
     CDS             complement(20078..21064)
                     /gene="plsX"
                     /locus_tag="O0394_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCY0498933.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNHDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            complement(21069..21641)
                     /gene="fapR"
                     /locus_tag="O0394_000834"
     CDS             complement(21069..21641)
                     /gene="fapR"
                     /locus_tag="O0394_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HCY0498934.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            complement(21844..23904)
                     /gene="recG"
                     /locus_tag="O0394_000835"
     CDS             complement(21844..23904)
                     /gene="recG"
                     /locus_tag="O0394_000835"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCY0498935.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDIDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEINVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            complement(24094..25740)
                     /gene="fakA"
                     /locus_tag="O0394_000836"
     CDS             complement(24094..25740)
                     /gene="fakA"
                     /locus_tag="O0394_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HCY0498936.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            complement(25755..26129)
                     /locus_tag="O0394_000837"
     CDS             complement(25755..26129)
                     /locus_tag="O0394_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCY0498937.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
BASE COUNT         7904 a         5169 c         3536 g         9831 t
ORIGIN      
        1 actaagtgtg tctccttttt ttacagtata gatttgtgtt tttggagctt ctctaaaact
       61 gtagtaacca aagtaattat tagtaacttt attcgttttc ttctgattag aattttgagc
      121 ttccaagttt gcaattttaa tttctttagt aagttcattg ttattaataa ctagattgtt
      181 accttggctt gagtttttcg gagtatttga aatctttata tcttgattaa tttcatttcc
      241 gtttgaaatt gctgatttgt tgtctaactt taaacttgtg tccgatgttt taacagcacc
      301 ttcatttttt attttgtctt ttgtcgtatt tttattagca tttaactctg atttcgcgaa
      361 tacattttgc tcataccctc ttgtaaaatc tttagattta tcaatttcat ctgcatatgc
      421 tttgttcgac atacccaatg ccaaaaacat acctattgaa attgacaaaa ttccaatact
      481 aacttttcta attgaatagc gtactttact ttgttgttta ttcatgatga aacactcctt
      541 acaataatat acaaacatta taacatagtg cgctatatat tttaattaaa attttctttg
      601 ttttataaag ctattgtagg aggtaaaata attaacttgt ctttccaaaa tatgaaaagt
      661 gtactaaaat tcatcgcacg acaaatagcc catttccgat acttttataa agtatggaat
      721 gggctatagc catttatatc atcttttaac tttatttatt aacagttaat aatgattcat
      781 aaatacctgc ttctttagca gcttcaatta atgttgaacc aatttctgaa ggtgttgccg
      841 ctgttttcac accacaacta tttaatgttt taattttctc ttcagcagta cctttaccac
      901 ctgaaatgat tgcaccagca tgtcccatac gttttccagg aggtgctgtt tgtccaccga
      961 taaagcctac aactggtttt gtcatattcg ctttaatcca ttcagctgct tcttcttcag
     1021 ccgtaccacc gatttcacca atcataacaa ctgctttcgt ttcgtcatct tcattgaatg
     1081 cttttaaaac atcaataaag tttgttccgt tgactgggtc tccaccaata ccaacagctg
     1141 tagtttgacc aataccttct tcagtcaatt ggtgcactgc ttcatatgtt aatgtacctg
     1201 aacgagatac tacaccaaca tgaccttttt tgtgaatata gccaggcata ataccaattt
     1261 tacattcatc tgctgtaatc acacctggac agttcggacc aactaaacgt gtttttctac
     1321 cttgtaagta gcgtttaact ttaaccatgt ctaatacagg aatatgttca gtgatacaaa
     1381 taaccatatc taagtctgca tcagctgctt ctaaaattga gtctgcagca aatggtgctg
     1441 gaacgtaaat gactgaaacc gttgccccag tttcattttt agcttcttca acagtgttga
     1501 aaacaggaac gccttcaaca acttgaccac ctttaccagg cgtcacacct gctactattt
     1561 tcgtaccata atcaagcatt tgttttgtat ggaaaagggc agtagaccct gtaatacctt
     1621 gtaccattac tttagtattc ttatctataa atacactcat cttagtgctc ccatcctttc
     1681 cttatgcttc tttgactagt ttaacaattt tttgtgcacc ttcagccatt gttgctgctg
     1741 gttcaattgc taatcctgag tcttttaaga tttttttacc taactcaaca tttgtacctt
     1801 ctaggcgtac aactagtggt aaagttaaat ctacttcttt tacagcttca acgatacctt
     1861 ctgcgataac atcacatttc ataatgccac cgaaaatgtt tacaaaaata cctttaacat
     1921 tttcatcacc taaaatgatt ttaaatgctt cagttacttt ttctctagta gcgcttccgc
     1981 ctgcatctaa gaaattggct gggtttccac cgaaatgatt aatcgtatcc attgttgcca
     2041 tggctaaacc tgcaccatta accatacatc cgatgtcacc atctaatgca atgtatgata
     2101 aatcatgttt agacgcttca atctctttcg gatcttcttc ttctaaatca cgtaattcta
     2161 caacatcttt atgtctgaat aatgcattat catcaaaatt aattttagca tctaatgcca
     2221 atacatcacc atcagctgtt gtaactaatg ggttgatttc tacgattgaa caatcttttt
     2281 caatgaatac attataaagt gctaataaga atttagcagc tttgttaaca gattctttag
     2341 gaatattaat attaaacgca attcgtcttg cttggaatgg tgaaagtccg attactggat
     2401 cgatagtttc tttgaagatc ttttcaggag tcttcgcagc aacttcttca atctcagtgc
     2461 ccccttcttc agacgccatc aatgttactt ggtcagtcgc acgatcaata acgaatccaa
     2521 cgtaatattc tttttgaata gcacaacctt cttcgatata taaacgctta atttctttac
     2581 cttctggacc agtttgatgt gtcaccaaag ttttccctaa taattctttt gcatatgttt
     2641 ctacctcaga taaagattta gcaattttta ctccgcctgc tttacctcta cctccagcat
     2701 gaatttgtgc ttttacaaca taaacatcag aatttaattc ttttgctttc tccaccgctt
     2761 cttcagcagt aaatgctact cgtccttctg gaactgcaac gcccattgaa cgaaatattt
     2821 ctttaccttg atactcgtgg atattcatct tccatcctcc tgttacttag gttaagttcc
     2881 cttacaatta taaaaaatgt aagcgctatt gtaaacttaa atgctacttt tttatcattt
     2941 aattgaattt tacgatttac agtaacgatt ttataggttc aaagcttttt ctatgctctt
     3001 tcataatgcc aatatcatcg attgctagta aatgttgttt ggtaccgtaa cccgcgtttt
     3061 tttcaaaacc atattcagga taatctttag ataactgtgt catataatca tcacgaaaaa
     3121 cctttgccat gatacttgca gctgcaatgg acacacttct tgcatcaccc ttgattaaag
     3181 atacttgagg cagtgcatta tcaagcgtca tcgcgtctat caataaatgc gttggttgta
     3241 ctgataatcc atcaatagct cgctgcatgg cgatttgagt agctttataa atattaaatt
     3301 catctatttc ttcagctgtc gcgatcccat atgcaaaagc agtaacttca ttttttagtg
     3361 cttcatttaa ttctagacgt ttcgtaacag gtactttttt cgagtcatca aggcccaaat
     3421 aattgtgatt tgaatttaaa attgttgcgc atgcaacgac tggacctgct aaaggtcctc
     3481 ttccaacttc atcaatccca caaataatag cattaggatg ctcttttaat atttcatttt
     3541 caaagtaagt catttcaaca tacttttctt ttaaagcttg ttctttttct aacgcttttc
     3601 tgcgcctagc tatggcattt tgaacacctt ttcgctcatc taaaaagcat tcatgatttt
     3661 ctaattcttc tattgtatta accgcattaa tcaactgcgt aacttcttta attgttagcg
     3721 tcatttgcta attcctcagt catatcttta aaaatatcaa aacaataatt tcctatttta
     3781 gcatttcgaa tatcataaat aatcagttca atgactgctt cgtaatcaat ttcattacca
     3841 cgtcgaatta agccacgttt tttccctatc gcatcaaacc acgctatgat ttctgcatct
     3901 tcaggaactt caatattata atgtgacttt aatcgcgcta aatcattttg aattaaaaag
     3961 tttaatccat agatggcaac ttcatctaag tgcacaatac tatcttttat cgcaccagtt
     4021 aaactcaact tcttaccgac ttcttcatct tcaaatttag gccaaagtat ccctggtgtg
     4081 tctaatagtt gtaatgcatt accaacttta atccattgtt gttgtttggt cacacctggt
     4141 ttattaccag tctgcgcaat actacgcttt gccagtttat ttattaatgt ggatttacca
     4201 acatttggaa ttccaacgat cattgctctt atcgctctag gtttaagtcc tttcgctttt
     4261 tcgcgttcaa atttttcagc agtcgcctta attgctgcag cttccacttt ctttaaattt
     4321 ttaccgtgct tagcatccac tgatacagga tagtatcctt tatcaataaa aaattgttcc
     4381 cattttgaca tctcatttaa attagacata tcttttttat ttaatataac aacacgtggt
     4441 ttttggttaa taacttcatc tatcataggg tttcttgaac tatatggaat tcttgcatct
     4501 actagttcaa acactacatc tacttttttt aattgttcac ttacttctct tttggctttc
     4561 gccatatgtc ctggatacca ttgaataacc attcaattca ccttactttc gtcaattctt
     4621 ttctcattat taaaatctat aacctgttaa ttatagaaca aattcattaa ttcaactata
     4681 tttattttaa cattgtcaca cttcttaaca atgctttaga tttaggtata agtgattatt
     4741 tcaagttttc tacaattaaa aatcatcatt tgttaaatct aagttactta ttaagtgaca
     4801 ttcatcttta tcagtatgac atttttatta ttcaatatat ttgtttaatg ataaactgaa
     4861 aatgatccaa agtttaagta actatgataa ggtatctgtt ccacagtcat cttcgtcttt
     4921 acctatcaag aacctcatta actttagttt cagttaaact taattttaaa tttgaaagtt
     4981 gaggataatg ttgtgttaaa aaagtggcta aattcaaacg tcaaacaatt ctttgttata
     5041 actttcatta gtgtaatatt aacgcttatt ttattttcta ctcatatcta tgattatatt
     5101 gtgaatggta ctgtttttag cggggctgga gatggattcc gtcaaatgat gccatttcaa
     5161 atgtatttgt atgaacatct acgtagtttt tctagtttat atgatgcatc gtttggatta
     5221 ggtggcgatt atatgaaagg actatcatat tattattcgc tgtcaccttt aatgtggcta
     5281 aattttctat tcattaaaat aggagaaacg gttggtatat ttaatccgac gacaatacat
     5341 ttttggccga caaaccaact tattatggct atgatacgag ctatcataac atttgtcgtg
     5401 accttctact tatttaaaat attacacttt aaacgctcag caaatatgat cgctacgatt
     5461 ttatacggca tgtcaactgt cgttatatac tttaatttta cttggtcatt ttatggaaat
     5521 ttattatatt tattgccatt atcgattctt ggtttggaaa gatattttca acaacgcaaa
     5581 atcggtattt tcattgttgc gatagcctta acactattta gcaattttta tttcagttat
     5641 tatcaagcta ttattatagg ttgctactat ttatatcgac tcattttcac ttacaaatat
     5701 gacattgtct ctagaacaca aaaattaatt tgcgtcatat ctgctacagt tttgagtgtg
     5761 ttatcaagtg tatttggttt attcactggc atttctgcgt ttttggaaaa tgacagaaag
     5821 caaaatccca atgttgatat accgtttttg acaccacttg attatcatta ttttttcttt
     5881 agcgatggat tttatattac gatttcaatt cttactatcg ttgcattatt gtcattcaaa
     5941 ctgtatcgtt tttactttta tagacttttc gcaatagtaa catggatatt atttataggt
     6001 tcattatcac agtatttcga cagtgctttt aatggttttt catttccaga aaggcgttgg
     6061 gtgtatatct tagcactatc atcaagtgct ctttgcggat tgtttattca acatttatca
     6121 acattaaata tgaaatatta tttaatcaga acaatacccg tatgcatcat cgcaatactt
     6181 tatgtattac tatcaccgac acacccactt gcacttatag taggtattat cctgctaata
     6241 gtgcttgccg ttattttaaa atttagttta tggcgttata aaaaattaac cgttgcaata
     6301 ttagtattaa tcgttatgat tcaacaaatc gtcattttag ataacaacaa aaacatggca
     6361 atcaaacctt atcaacaatc attatcaacg ttgaaacaac atgattacca tagtaactat
     6421 gtaaaccagc ttataaaaaa gataaatcaa aatgcaacag gctcatttaa tcgcattgat
     6481 tatatgtcag actatgcatt aaattcacca tttatatatc attataatgg catttcatta
     6541 tattctagta tttttaatgg agacatttta aaatattatg acaagacact ccaaattaat
     6601 atgccaatcg ataaaaacag cacttataga ttacttggca atcgtcaaaa tttactatca
     6661 ctttggaatg ttaatgatcg aattagagtg aatcatgatg acaacttacc atatggattt
     6721 aaaattaagt ctgaacacaa agacaataaa gttcgttgga ttcattctaa aaataccatc
     6781 cattatccaa gtgcacatat tacaaataag gtcttttcca ataaagaatt aaaatctcca
     6841 ttagataaag aacaagcaat gttgcaaggg attgtttcta acaatattaa agatgttaat
     6901 acacatttta aagccaataa aaatttacta tcagattcaa caattaaatt aaatagtgca
     6961 gcctggcaat ctcctacaaa acatttatta caagttaaac aaaataatgg tggtctaact
     7021 gtacagttgc caaaatcagt ttctaatcaa tttaaagatt tgtattttga aatggattta
     7081 gaattacttt cgccggataa agctcatgat gttaaagtga atgaatatac acaagaaaga
     7141 aataaactca cttataaata tcgacgcgtt gtaacacccg taacgatacg cattaaagct
     7201 ccagatagaa ttagattatc attgcctaaa ggtaagtatc gagtaaattt aaaagggata
     7261 tacggcgaag attataccac gcttaaagac gcttcaaatt cattagaagc tgtcaaagtt
     7321 agtaagacaa agcatggtta tactattact aaaaataaaa attcatctgg gtatattgtt
     7381 ttgccaacag catataatca aggtatgaaa gcgacatcag gtgatcaaag tcttaaagtt
     7441 gaacaagtaa atggtgttat gaccggcatt aaagcaccta aaaatataac aaagattcaa
     7501 ttgagctata ccccaccata ctattattta cttataacaa ttactatatt tggcattata
     7561 tgtagtatta ttttcacgag atgggcaaga caaaaataat gttgctacgc atatatttaa
     7621 taaatctact aagtcctttt caatggcact tagtagattt ttgtttatat aaccattttg
     7681 agcaaacacg ttatgaatag agaaggaata aacaattaca ttatcaactt tacattaact
     7741 aaacagcaaa aaaagccatc tatatcactc cataaaagtg acctagatgg ctttattgtt
     7801 tgagtcgaat ttgactcata catagctttg tttaaatgct gattaacgaa tttcttggat
     7861 tctagcagct ttaccacgta aactacgtaa gtaatataat ttagcacgac gtactttacc
     7921 acgacgttta acttcgattt tttcaatttt tggtgtgtgt aatgggaatg tacgttccac
     7981 gccaacacct gatgaaattt tacgaactgt aaacgtttca gaaacgccac cgccacgacg
     8041 tttaattaca acgccttcga atacttggat acgctcacga gtaccctcaa tgatacgtac
     8101 gtgtacacgt aaagtatcac caggacggaa acttggtaag tctgtacgta attgtgattt
     8161 agttactgct tcgattaatt tgtgatttgt cattataaat tctcctcttc aacctatgtt
     8221 cttgcctcga caaatatata gcagcggatc atagtgattt tagtgtttcc acactcagat
     8281 tatgttagca caatactaac cttttttcaa tcctatttta tatctttcta atatttgctt
     8341 atctgcatta gttaatggat atttttcaat taagtcaggt cttttattat atgtgcggat
     8401 caacttttgc tcatgtctcc atgcatcaat attggcatga tttccagaca ataaaacatc
     8461 tggaactgtt agacccttaa attcacgcgg acgtgtatat tgcggaaact ctaataaccc
     8521 atctgaaaat gaatcgtctt gatgtgactg ttcattacct aaaacacctg gaatcagtct
     8581 aacaatagca tcagtcatgg tcatcgctgg caattctcca ccagttaaaa catagtcacc
     8641 cattgatatt tcatctgtga caagatgtgt tcggatacgt tcatcgtaac cttcataatg
     8701 tccgcatatg aaaacgatgt ggtcggcctt gcttaattca acagctttct gatgtgaaaa
     8761 tggctcgcct tgtggacaca ttaaaataac gcgtgtttgt tctgtgacat ctaagtcttc
     8821 catcgcatta aaaacaggtt caggctttaa caccatacct tgtccgccac catacggata
     8881 atcatctact tggttgtgct tgttaattgc ataatctcta aaattaaccg tattgatttg
     8941 taatttattg ttttcttggg cacgtttcat aattgaatga tttaaaacac catcaaacat
     9001 ttcaggaaat aaagttaaat aatcaatttt cattaatcca acaatccttc cattggcgtg
     9061 atgataattt ttttattttc aacatccact tcttttacaa catcagcaat ataaggaatc
     9121 aaatattctt tagatccttt aatcacccac acatcattcg cacctgtttc aaatatatta
     9181 attacacgac ctattggtgt ttcttgatca tcaaaaactg tacatcctat aatatctgaa
     9241 taataaaatt cattttcctc aagtacgata tcttcatgat cacgctcttg ataaatagaa
     9301 ctccctttta aatgctcaat atcattaata ttattaatac cttcaaatgt aagcatatga
     9361 agccctttat gcactctatg tgacttaaca gtatattcaa ggtcgttatt gttatgcaca
     9421 actgtcagca cttgaccggg ttgaaaacga acgtctgtaa agtctgaatt ggattttact
     9481 ttaatttcac ctttaatacc atgtgtgtta acaatttgac caacttcaac tctcattcta
     9541 ttacctccaa ctctatgctt tgaacacttc tattttaatt gaaaataatt caaagaaaaa
     9601 gggcaccatt atggtaccca atacgttcta tattactaac atgtgatatt cccaacgtat
     9661 cattgagtaa taactgtcag tgtaaacgag ttattcttat tgttagaaca attttacgct
     9721 aaattactta gctttctttt gttcgtcaaa ttttttcata ataccttctt ttgataagat
     9781 attgtgaact gtatcagttg gtttcgcacc atcatttaac cattttaaag ctaacgcttc
     9841 gtcaacttta atttctggag cattagcgct cgttgggtta taagtaccga tttgttcgat
     9901 gatacgtccg tcacgtggag aacgagcatc tgctactacg atacgataga atggatttct
     9961 ttttgaacct aaacgtgtta aacgaatttt aactgccatt tataaaatct cctttaagtc
    10021 atgttttaat ttttattttc tacaagaaat aatattaaca ggtgtatgaa tgtttgtaaa
    10081 gagttttttc tttaccagga aaattattga acctttttct tacaataata actatgtgat
    10141 tactaaatta gactgttaat aggttataaa aattcatagt aaatttcacc acctacgtgt
    10201 taagttaatt tatctcgtaa ggacatgaat ggtaccactt ctaccgcaca cgttccccca
    10261 atataactta ttaatattag caacaataac ctcatgttct agcaaatcag caatttcagt
    10321 ttttaattat tattgtgtat ctaaaaatat gcaccccata aagttatatt ctttagtata
    10381 actttcggga tgcacatctc attgcaacaa aaatcagcgt gttatattaa acattaaaac
    10441 ggtaaattca taccttttaa catattttgc atttgattgc gtttaccttt tttaccttta
    10501 ccgccaccag tgaactgttt catcattttc ttcatatcgt taaattgttt catcaaacga
    10561 ttgacttctt gtaatgaacg accagaccct ttagcaatac gctttttacg tgatacattc
    10621 aatgtgtctg gattgtttct ttcagccggc gtcattgact ggataatcgc tttaatatga
    10681 tcaatttgct tttcactcat attaagctta tctagccctt tcattttatt catacctgga
    10741 atcattttca taatatcatc cagtggtcct agatttttca cctgatcaag ttgttctaaa
    10801 aaatcatcta aagtaaacga tgactcacgc atctttttct ctaaatcttt tgctttttct
    10861 tgatccacat cttgttgcgc tttttcaatt aaacttaaca catcacccat acctaaaata
    10921 cgtgatgcca tacgttcagg atggaatagc tctaaaccat ctaacttttc actcatacca
    10981 acaaatttaa ttggtttttg tgtcaccgaa cgaatagata aagctgcacc accacgtgta
    11041 tcaccatcta atttagttaa ggtaacacct gtgacatcaa gttgatcgtc aaaagattct
    11101 gcaacattga cagcatcttg acccgtcatt gaatcgacaa ctaacataat ttcgtttggt
    11161 ttagcaatgt cttttacttc ttttaattcg ttcatcaatg cttcatcgat gtgtaatcga
    11221 cctgctgtat caatgattac aaagtctaaa tgttcttctt tagcatgttt taatgcatta
    11281 gttacaattt gttgtggctt tacttgatct ccttcactgt atacaggaat atcaatttgt
    11341 ttccctactg tttgtaattg atttatcgct gctggacgat aaatatctgc tgcaactaac
    11401 ataggttttt tgttgtattt tttacgcatc aataatgcta atttacctgc agttgttgtt
    11461 ttaccagcac cttgtaaacc aaccatcata acaacagtag gtggtttatt tgacatatta
    11521 atcgacgtat tttctccacc catcaactgc gttaattcat cttgaactat tttaataact
    11581 tgttgccctg gtgttaatga ttgcattaca tcggaaccta atgcgcgttc tgatactgtt
    11641 ttaataaatt cttttaccac tttaaagttt acgtcagcct caagtaacgc taatcttact
    11701 tcacgcatca ttatctttat atcagcttca gtaagtttac ccttaccacg cattttttgc
    11761 atcgtcgctt gcaagcgttc tgataaccct tcaaatgcca tatcgatgtc ctccctcaat
    11821 ttatatctat tctaagtctt ctaattgttg aatataacgt tgtatttgtt ctggattact
    11881 taaatgttgt ttcatttcat catatatttc tcggcgttgc tcaaatttct ggtataattc
    11941 caatttcttt tcataatctt caactaaatc gccagttctt cttatattat cataaactgc
    12001 ttgtctactc acattaaaag tatctgcgat ttcacttaaa gaataatctt caagataaaa
    12061 taattccaaa taattacgtt gtttattcgt caataaggat tgataaaaat caaacaaata
    12121 attcattcgt aacgttttaa ctaaatcatt ttgacccatg attatcgtcc ttttcttctg
    12181 ttacaatttg atcattttca actgttgtta tttcttcatt ttgttcaatc atatcagcga
    12241 ataagccgta gacataactt tcagggttaa atggttgtaa gtcatctaat tgctcaccta
    12301 aacctacata tttaactggg atgtgcaatt cattacgaat ggctaatacg ataccacctt
    12361 tggctgtacc atctaatttc gttaatacaa tacctgtaac atttgttact tctttaaagt
    12421 ttctagcttg tgacaacgca ttctgaccag ttgtagcatc taaacatagt aatgcttcat
    12481 gaggcgcatc tggcactgct cgattaatta cacgcttaac tttttctaat tcttgcatta
    12541 gatttgtttt attttgtaaa cgtccagcgg tatcacagat taaaatatca acacctttgt
    12601 ttttagcggc attaatcgca tcatacataa cagcagctgg atcagaacct tcactttggc
    12661 taattacgtc tacaccaaca cgttcgcccc aaactttcaa ttgatcaata gcacccgctc
    12721 taaaagtatc gcccgcagct aacattactt ttttaccttc cattttatat cggtaagcta
    12781 attttccaat tgttgttgtt ttaccaacac cattcacacc aaccattaaa atgacattta
    12841 aacgaccatc ttctaagttc atagcttctg aattatcatc ttcttgatgg taaatctcta
    12901 cgattttttc aacaatgact tcacgcaaat cttcagtatc ttgaatatta cgtcgttgtg
    12961 cttccatacg taattcttca gttaacgtca tcactgtatt aaaaccgacg tctgcagtga
    13021 ttaacatttc ttctaaagct tcaaaaaagt cttcatctac tttacgatat ctcgctatca
    13081 aattatttaa ttgttcttgg aaattttgac gagatttttc gagtcctgct ttaaatttag
    13141 cacccatttt ttgagcttca atttcttcaa aatcttcaat tgatattaag ccatcgtcat
    13201 caaaatccgc ttcactcaac ttgcgtggct tctttttcac ttcagcattt tctgctaaat
    13261 catttgcgtc ctgcgttgaa ccttcagaat gtgtatcttc taatttgtct tgaccttgtt
    13321 cttctgttaa ggatttaact tcttcatttt ctttatttgt tgcaaactta tcttttaagc
    13381 gtttaaaaaa gctcattatt gctcctcctt caacacatca tctattgtat ttaaattcac
    13441 actcacaagt ttagtaacac ctgattcttg cattgttaca ccgtataacc tatctgcaaa
    13501 ttccattgtt cctttacggt gtgtaataac aatgaattgt gtttcgtctg ataactcatt
    13561 taaatatttt gcgtatctaa taacatttgc ttcatctagt gcagcttcaa cctcatctaa
    13621 tataacaaaa ggtgcagatc ttacttttaa aattgcaaat agtaaagcaa tagcagttaa
    13681 tgcacgctca ccaccactca gtaacgataa atgttgcaac tttttacccg gtggttgtac
    13741 cacaatatca ataccagctg ttaaataatc ggcttcagtt aattgcaatt ctgcatcgcc
    13801 tccaccaaac aattgtttga acacagctgt aaaatgtcct tgaatagcat ggaaagtttc
    13861 tttaaatctt tcagtaacct cttgatccat ttcacttata atttgctcta atgtttcttt
    13921 agctttacga agatctgtac gttgttcact taaaaatgta taacgttcat ttaactcttc
    13981 aaattgttca attgcattta agtttacagg acctagttca tcaatcgaca tcttcattaa
    14041 cttaactttt ttacgtaatg cgtcaatcga ttcatcactc gtatattcag atttcgcacg
    14101 ttcaacagtc aattgatatt catcatttaa atgatctatc gcatgatgaa ttaatacatc
    14161 tagctttgat tgttcagctt taatatcttg gtagtgattt tcgatagcta aaatatcttg
    14221 gtgacaaact tgtagtttag cttcttgcgc atcgatttgt tcattcaact caatacgttg
    14281 ttgtttcaat ttatctaatt catctgataa gcgtgttctc gtttcttgtt gaccattaat
    14341 ttgatcttta atattttgaa aagcttgttc gcccatcact tcatccgaat taaagaatgc
    14401 aattttttct ttaacatctt ttaattgatg tttagtttgt tgattttgat tatttaatcg
    14461 atctattgtc tgttgttgtg ttttaatacg ctctttaacc acagcaagat cagattgttt
    14521 ctgatgtaag gtttgttgtg ttttagtaac gctttcctta ccttctttag aaagttttgt
    14581 gtagcgttca atttcatctt ctagtcgttt taaagatgct ttaatacttt ctagataagt
    14641 ttctttttca ctcaaagttt gtcgactttt gtcactcgta taaccatcat ttttttcaaa
    14701 ttcgaattct tcatgatcat tttttatttg tgtttcttgt gtagttaatc tatcgagctc
    14761 catttcaaaa tgatgcactt gctctttaag tgtattatgc ttttgacttt tttcaaaata
    14821 cagttcactt aattgatcac tttttatctt caactcttta aattgttgtt caaatgattc
    14881 tgtttgacgc aagtaatctt ctaattggtg tctcattgtt gtcaactcgt ctttttgaga
    14941 cagaatactt tttgacttac gagcgccacc accagtcata gaaccaccag gatttacaat
    15001 atcaccttcc aaagtaacaa tacgagttcg atatttaatc gcacgtgcca attcatttgc
    15061 atgctttaaa tgatcaacga taatcgtatt acctaataaa ttcccgataa tattttgata
    15121 ttctggtgct actttaactg cttccgaagc gatactaata aatccgtttg cctctttagc
    15181 aatagattta atatcagtcg ctaccactct actctgtata acatttaatg gtaaaaacgt
    15241 cgcacgacct aaattacgtt cttttaaaaa ttgaatagcc tggcgtccat ctttttctga
    15301 atctacaatg acatgttgta atgaagcacc taatgctgtt tcaattgcct gagttaattt
    15361 agatggcaca tcaataattt ccgcaactgc accataaata ccctttaatt ctttattttt
    15421 agctttcaaa atatgtttga cgccattgaa aaaataagta tattcttcct cttgcgttgc
    15481 caaactatca atacgtgttt tcattttttc ggtatatcga tatgcttgat acaatttctc
    15541 ttcgtattca ttttgtgctt tttttgtatc agtgaggtct ttttctatat ttttaatttc
    15601 tttatctaca gcagaaagtt ctttgttggt ctgttgatat tctttttttg tcgtttttat
    15661 ttgaccctga atatctttca attgctcaaa aacttcaact aatcgagaat ctagtcttga
    15721 ttttttagcc tcattctctt ctatagtatg ctttaaaaaa cgaatatcat tgttaacatc
    15781 tgattgctct gacattaatg tatagtattc gtttttaatt tcttccaatt tttcatcatg
    15841 tgcttcgtct gaaacatata gttgttcttc aagttcacga atgacagcat tgagttcttt
    15901 ttgtttactt ttcagagact tataagtatc ttgagcttca gaaatctcat ttgatatatt
    15961 ttctaaaagc tccattaaat tttcttgttc ttcttcatat cgtgcatttg tttcagattg
    16021 atttttctta cgttcttcca aaacatttaa ttgtcccgta tatttttcaa aggcttccgt
    16081 agcttttact aattgataat taagcgattc aacatcatta tcaagttgat gacgtttacc
    16141 tttatattgt tgaatttgtt ggcttaaacg ttgcttgtca gcttctttat ttgcttgttg
    16201 gccttgtaaa tcatttaaac gttgatctaa ttgtctattg tcatttgtat attgatcaat
    16261 atcgtgcact gtaactacaa tgtcactatg tttcatttga tgtgaaagtg tcttatattc
    16321 tttagctata gctgcctcct cttttagagg ttctacgcga ccttccaaat catataaaat
    16381 gtcttctact ctcgttaaat tatcttctgt ttggtcaagt ttatttaatg attcagcctt
    16441 acgtttttta tatttaagta caccagccga ttcttcaata atttgacgtc tatcaattgg
    16501 tttagcattt agtatttcat caactctacc ttgcgagata atgctatacg cttcttttcc
    16561 caatccagaa tctaaaaata aatcggcaat atcttttaat cttgcacggt cattatttat
    16621 gtagtactca ctttcaccac ttcgatacaa tcttcttgtt acaataactt cgttttcatc
    16681 aacactgagc tttttagaat gattatctaa tcttaactgt acttcagcat aattttgagc
    16741 tttgcgatgt tctgcacctg agaagataat atcttccatt tttgagccac gtaatgattt
    16801 agccgattgt tcacccaaca cccatttaat agcatctgta atattacttt taccgcttcc
    16861 atttggacca acaattgcag ttacaccttt atcgaattga acattggttt gatctgcaaa
    16921 agacttaaat ccaatggcat ctattgattt taaataaacc atcctaaact ccttatcaca
    16981 attttaaaga acacatgtat acgatatttt agtatttgta tattaaattt cgaagttgtt
    17041 ccataaaata ttaaaaattc ttccttaaat cctaaatcga acacctatat gtcgtatcat
    17101 ataaatttct atttaatttg ttttaattgc ttataggcac tttcagcagc acgttgttct
    17161 gattcttttt tcgttttccc tttaccttca gctattgctt ccccttgcag aataacttct
    17221 gaagtgaata gacgatgatg tgccggtccc tcttctttta ttaaattata ggttacatca
    17281 cctttatttt gctggtgcac atattcttgg aattgtgttt taaaatctac cacgcctaat
    17341 aactcatttt gttctacatg tgggaaaatg actttctcag caaatttcca aactatatct
    17401 agtccttgat ccaaatacaa tgccccaata aatgcttcga atgcatctga tattaatgat
    17461 ggtcttgtac gtccccctgt tttctcttca cctttaccaa gtaaaatcat ttcgttcaat
    17521 ccaattttat tcgcaaatat tacaagtgag ggctcacata caatagtggc acgcattttt
    17581 gttaaattcc cttctggcaa gttgggatgt ttatcaaata aatatcgtga aaccgtcaat
    17641 tctaataccg catcacccaa aaactctaaa cgctcattat ggtctaaacg attcatatta
    17701 aaatcattaa taaaactcga atgcgaaaat gcttgttggt ataaatcaat attttgataa
    17761 gtaaagccta actctgtcat tttagtatca aagcgctttc taaaacgatt aactatctca
    17821 cttttctttt gtttagacat tattttatct cctttatgtg ttgctcttga atcatcttta
    17881 attttacgtt aaaactattg ataattaaag aaaaaaactg agtcgacaat actgacgacc
    17941 cagatgtaag atttattatt tttcaagact gttaataaat ttaacagcat caccaacagt
    18001 gttgattttt tcagcttctt catcaggaat ttcagtacca aactcgtctt ctaattccat
    18061 tactaattca gcgatatcaa gtgagtcagc gcctaaatca tctttgaaag atgcatcttc
    18121 agttacttta tcagcgtcta cacctaaacg gtcaacgatg atatctttta ctttatcgaa
    18181 attttccacg tcgattcacc tcctttaaaa atgcctatac atagactact atattttccc
    18241 attttaaatt ctaaaaaaca agtagaaatt ttagaaacac ttatcaattt attgattagt
    18301 gtttcttagg gagaaattct ggaaccttat ctaggttgct tagaatttcc cccgcaaagt
    18361 tgactaggtc agacaattct ccattggatg accagtcaac cactgcgtca atgagcttta
    18421 gctcaaatat attacatgta cattccacca tttacatgga ttgtttgacc tgtaatatat
    18481 tttgctttgt ctgatgctaa gaacgctact gtattagcaa tatctgtgtc ttgaccaaaa
    18541 cgtgctaacg gaatttgagt caacatttgt tctttaagct catcacttaa agcatctgtc
    18601 atatcagaaa caataaaacc aggtgcaact gcatttacag tgataccacg agatgctaat
    18661 tcacgcgccg cagatttagt taaaccaata acacctgctt ttgttgcaac atagtttgct
    18721 tgtcccggat tacctactgc tccaacaaca cttgataaat tgatgatagc accactacgt
    18781 tgtcttaaca tttgtggtgt tgctttttgg atacagttaa atacaccttt taagtttgtg
    18841 tcaataacat catcccactc ttgttctttc atacgcatta ataaattatc gcgagtaata
    18901 cctgcattat ttactaaaac atctaaagaa ccaaattggc taactacttc tttaatcatt
    18961 gctttaactt catcagcatc ggcaacattt gcttgaatcg caaaactgtc aacaccttta
    19021 gctttgattt cttcgactac tgcttcagct ttctctttgc tgcctgcata gtttactgct
    19081 acattatatc cttcttctgc taattgtaac gcaatactac gtccaattcc tcttgatgca
    19141 cctgttacta aagcactctt agtcattttc attccatcct ttcacatctt ctaaagtttg
    19201 aattgatgtt aacttaacat ctctatttat ttttttaatt aagccagata aaacttttcc
    19261 aggaccaatt tcaataaaat gatcaacacc ttggtctatt agccattctg ttgagttaat
    19321 gaattgtact ggtgaatata attgcttgac catattagat ttaattactt ctttgtcagt
    19381 ttcaccttgc gcatttacat tttgaactac aggaaactta gcatcacgcc attcaaattg
    19441 attaatgtaa cttgaaaaat cttcttcaat cactttcatt agcgatgaat ggaatggtcc
    19501 agatactgct aaaggcatga cacgttttgc acctaatgat ttaccttttt ctactagctc
    19561 atcaattaaa gctttgtgac ctgaaacaac aatttgacct gggcaattaa tgtttgctgg
    19621 ttcaattatt ttgtcatcag atgataatga cttacaaatt tcatcgactt tatcaaaatc
    19681 taatcccaat actgcagcca tgcttcctac accagtagga aatgcttgcg ccattaattg
    19741 accacgtttt ctaacaattt taactgcatc ttcaaatgat aatacgtcag ctgcaactaa
    19801 acttgaatat tcacctaaac tatgccccat agtaaaatca ggattcaaat tttttagcgc
    19861 tgctaataat gccgaactat gcgtcaataa agctggttgt gtgttttcag tttcacccaa
    19921 tttaccttct tcatcagtaa acattgtctc taaaatatca aagtctaatg tgttcgctgc
    19981 tgaagttaaa atttcagttg cttgatcatt gttgttaaac aaatcttgcg ccataccaac
    20041 tttttgggca ccttgtcccg gaaaaataat tgctgtttta ctcatttgat tcacctacag
    20101 tctctttcat tgtttgtaca atattttgtt ctcctgcgat tttcgcttgt ctaattgcag
    20161 aataaaaagc tttagcattt gaactaccgt gtgctttaac tacagtacct tccaatccta
    20221 ataatacgga accaccgtat tctgagtaat ccatcttttt agcgaattca gctaaatctt
    20281 tcttcaatat tgcacctgct aatttatttt tagtactact cataatcgta tcttttaaca
    20341 ttttaccgat tgattttgca gtaccttcta aatttttaag gaccatgttc ccagtatagc
    20401 catcggtaac tacaacatct gtatcgccat ccattaatgt cttcgcttca atattcccaa
    20461 caaaattcaa tgaatgatca tgatttaata actcatatga ttttttcgtt aaactattac
    20521 ctttagctgg ctcggttcct atatttaata atgagatttt cggattatca atacctctaa
    20581 ttttttgagc ataaatatcc cctagttgcg catactgtaa taagtgttca ggtttagcat
    20641 cagcatttgc accaacgtct aaaaagacaa aaccttttcc atcaatcgtt ggcaatgtta
    20701 ctactaaagc cggtctagct acacctttaa tacgtccaac aatgaataaa ccagctgaca
    20761 ttaaagcacc agtattacct gctgacacac atccatctgc ttcaccagat ttcacagctt
    20821 cagccatttt taccattgag ctatcttttt tacgtttaat cgctctaaca ggctcatctt
    20881 ccatttcaat cttttcagaa caatgtctaa attcgattcg ttcatggttc agattatact
    20941 ttttttcgtc accgaaaagt ataatttcta gatctttaaa gtcttcaaca gccttttgta
    21001 cggcttctaa tacgatatca ggcgcattgt cgccacccat catatcaatt gctaatttaa
    21061 ccataatttt atcctcgctt atcataaaac attttaaaat ttcctttgaa aactaatgta
    21121 tgtttaacat atgactttac ttcgacgtaa taatgttttg cagtttgatt tacaactcgt
    21181 gcttctgctc ttaccgtatc atttaatttt actttttcaa taaattgaat gctactctca
    21241 tgagttaaaa ctgttggttg cttaattagc gcaacacata acgaatttgc ctgagcaaac
    21301 agcacatgac cacgcgcaat tccagtttta tgaaaaacag aatccgatgt aatatctaaa
    21361 attgattgcg ctttaacatt tggattgact tgaatcaaat caccaataaa ttcttgttct
    21421 tcaatagaac ttatttggtc ataattcttt tcagcaacta atttaatacg cttccttaat
    21481 tctggtatgt ttaaataagt gcgatctaaa cgaattgttt gtatactcac ttgaaataag
    21541 tcgcttagtt catggtctgt gatgaaggga ttgctatcaa tttgttgtct gattgcttct
    21601 ctacgtttat ctttctttag tttcaacgtc tcacccctca ttttttagta cctagtctta
    21661 aacattcctc acatatcata acactttcta tttaaccgac tcaacaaata actgtaatat
    21721 ttagctatta tttagtcgaa aatttataaa ttgttaaaat accactttac atttaaatag
    21781 ttcaagtgtc atatcactca attaacgaag tgttgcactc aaattgacaa atcagcatgg
    21841 caattagtca aaactacgat gtaataaatt ttcttcaaca aaatgacgta aatgttgata
    21901 cgtattttca aagaatacgc cagattgaat aagttcagct gcttcatcac gagcaacttc
    21961 taacatacga taatcttcaa ctaaattggc aactaagaaa tctggcaatc cactttgttt
    22021 aacaccaaag aaatctccag gaccacgcat ttctaagtct cgttcactca attcaaatcc
    22081 atccgttgtt tgtgtcataa ttgtcattct ttcaattcct gtttctgttt taggggatgc
    22141 aattaaaaca cagtaacttt gctggtcact tctacctaca cgaccgcgta actgatgtaa
    22201 agttgataat ccaaagcgat ccgcatcata aatcatcata aaagttgcat tcggtacatt
    22261 aacacctact tcaacaacag tagtagaaac taaaacattt atctcatgat tactaaactt
    22321 ttgcatgacc tcatcttttt catcggcaga taacttacca tgcaataacc ctacacggga
    22381 aacaccataa tactgttgta aagactcgta caatgcgaca acattttgaa catcttcgag
    22441 atgctcagaa ctttctatta gcgggcaaat gacatatgct tgacgacctt ttttcaactc
    22501 tgaggtcatt tgcatcaaaa ctttatcgta ttgctcatgc tttgcccaag tagtaatgat
    22561 aggtttacga ccttttggta attgtttaat tgaagacaca tccatctcac caaaaactga
    22621 tattgctagt gttcttggta tcggcgttgc tgtcataaat aacacattcg tcattgcacc
    22681 tttttctctt aaaagctggc gttgattcac accaaatcga tgttgttcat ctgtaattac
    22741 taaaccaaca ttatggaaaa tcacatcatc ttgaatcaaa gcatgggttc caattaaaca
    22801 atcaatcgta ccattttcaa gttgttctaa aagtatcttt cgtttcttac cttttactga
    22861 cccagttaac aatgcaacgt tcatagaatc tccaaataaa gccattaaac tttcagcatg
    22921 ttgctctgct aaaatttcag ttggtaccat caatgctgat tgataaccag cagtttttaa
    22981 cgcatacata caaattgcag caactactgt ttttcctgaa cctacatcac cttgaagtaa
    23041 tcgatgcata cgtattggtg cttttaaatc tctaaaaatt tcattaacac tggatttctg
    23101 tgcttcagtt agttcaaaag gtaaacgatc aataaatgat ttaacttggt ctatgtcata
    23161 atcaatttca attgcttcgt cagatgactt ttctaatcta tttagccatt gcatacgtaa
    23221 ttcgaataaa aacagttcag taaatgcata ggttctacga gcacgtaata aatcctcttt
    23281 acttttagga tgatgtaatg tgttcaaagt aaagtccaag gtctctaatt tatatttttc
    23341 tcttagttca tcagttaacc attcatgaat tgtcacatca tttaacgctt gtctaatttg
    23401 gtctcgtatt tgcttttgtt taataccttc cttaatacga tagactggtt ctaattgaac
    23461 atctgcgttt tcttgagttt gtgtcccttg tgaattaaag aaaaccctat taccagtaat
    23521 ttcctgttta accctattcc acttaccttt aacagttata gtttgattta attcgatttt
    23581 cttttttaaa tacggttgat tgaaaaaaat acatttgaca gcaatattat ttaccattaa
    23641 atgaacggtt aattttgatt tatttcttcc aaaaaatgca actactggag ctgtatatac
    23701 ttgtccttct atcgtaacgt tagattgatc ttctgcttga ttcaaatcaa tcactgtatt
    23761 atcttcatat ctagttggca aataaagaac aagatcttcc actgtatgaa tatttagttg
    23821 ttgcaatact tctattttct taggacctat accttttaat tgtaaaagag aatatggact
    23881 ttctattaag tttactttag ccaaaataaa tcacctatgt tttatttgat tttacgtatc
    23941 atttagtatg ttatttattt tatcatgact aagttgaaca tgacattgtt agcaactcga
    24001 gaacgatact tttaacccgc aatatcacta aaatagcaaa ataaaaaact accaactgat
    24061 gatttatcat tggtagtttt ttattttaaa tttttattct actgaaaaga aatattgata
    24121 aattggttgt ccaccttcat gaacttccac ttctacatct ggatattgct cttcgatcca
    24181 gtttatcatg ttatctgtaa ctgcttgctc tgcatcttga ccaataatca cagtcaatat
    24241 ttcactatct tctgctaaca tctcatttaa caactcagta accgttgtta attgatcact
    24301 ttggctgctt acaatcttat cttcaatcaa gcccataaac gcgtcttttt taatctcaac
    24361 gccatcaatt ttcgtatcac gaacagcgta cgttaatgaa ccagatttaa cgttatttac
    24421 tgaatcagcc atttgcgctt tattttcttc aagtgttgcg tccacatcat attggaatag
    24481 tgcgcttata ccttgaggaa tagatttcgt tggaataaca acagcttctg catcaacaat
    24541 actcgctgct tgttcacttg ccattaagat atttttatta ttcggtaaaa taattgcacg
    24601 tttacatttt gattgttcaa tgactttaac gatatcttct gtagaaggat tcatcgtttg
    24661 tccaccacta atgatatgtg tggcacccat tgatttaaat atctctgaaa taccttcacc
    24721 catagaaata gtaataattg ctgtttcaac cgtttccatt ttcggtttag ctgtgtgctg
    24781 ttcttttcga atcacttcac ggtgctgttc tctcatattt tcaaccttaa gtttaattaa
    24841 ttcaccatat tgttgaccat aattaaacac tttacctggg tattcggtat gcacgtgaac
    24901 tttcacaatt tcttcatcat taatgactaa taaagaatca ccaaattgac tcatatcttg
    24961 cctgaattct tgttcatcaa aggctttttt attctttcca aaacgaacca tcatttcagt
    25021 acaatagcca taaataatat cttcagtatt aattacacca tggaaatcat gttcatcatg
    25081 tacaaattca tctttatcta tctttgcaac tttggcttca actttttcac ctttaagcgc
    25141 ttttaagaat ccttcgtaaa cgcataacaa acctttaccg ccactatcaa caacaccaac
    25201 ttctttaagt acagctaata agtttggtgt gttttcaagt gattcattgg cttttacaat
    25261 aatgtactcc attaattcta tacaatcttc agtattattt gctttttcta ttgcagcttg
    25321 cgcagcatct tttgcaactg taagtattgt accttcaact ggtttcataa cagctttata
    25381 tgccgtttca acaccagctt gaaaactttc agctaacaat tttgaattaa tttcagattc
    25441 actttcaata tttttacaaa atcctctgaa taattgtgac aagatgacac cagagttacc
    25501 tcttgcaccc attagtaaac ctttcgagaa tgttttacct aattcgccga tatttttcga
    25561 caaattattc tctacttctt cgcgacctga agtcatagta agattcatat ttgttcctgt
    25621 atcaccatct ggcactggat acacattcaa agaatctacc aaatctgcat tgttagataa
    25681 attttgtgcc ccttgtataa tcatatcggc aaataattta ccattaattt tgctaatcat
    25741 ttcaagttgt cctcctaagc tttcttgcct gtattattca cacgtacacc ttgtacatat
    25801 atattgattg aatttactga tacattaagt gatttttcca aagtatattt cactgttgat
    25861 tgtacattat tggcaacttc agatattttc acaccgtaac ttacaataat gtacatatct
    25921 atatccacta cgccattatt ttcagttact ttgatgcctt tagcataatt ttcatgtcct
    25981 agtatttccg caataccatc tctaacttgt tgtctagatg ccatacctac aataccataa
    26041 cattcaacgg cctttccacc tacaaccgaa gcaatcactt cgtttgaaat atcaatttta
    26101 ccgtaatcat ttgaaatctc taatgtcata tctttgtccc tcctaaattt caaagtttat
    26161 tattcgtttt ccctaatcac ttttaaaatt ttaccactta gtgtagtcat aaacgcttta
    26221 agtttcattg attattttta taaaatatca atggtacaac ttatcagaat gacttacgct
    26281 taaatcacct attatatttt gagtaaactg aatttacact catgacgatt atctataggc
    26341 gttttctttt aatagatgta gtatcattaa ataaaaattt taaatgtttc attcacttat
    26401 ttctctaaaa tatcatacta cataacttca cagtctacaa
//
LOCUS       DAKTLH010000023        26335 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.023, whole genome shotgun sequence.
ACCESSION   DAKTLH010000023 DAKTLH010000000
VERSION     DAKTLH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26335
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.023"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            20..1282
                     /locus_tag="O0394_000838"
     CDS             20..1282
                     /locus_tag="O0394_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCY0498938.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            complement(1419..2510)
                     /locus_tag="O0394_000839"
     CDS             complement(1419..2510)
                     /locus_tag="O0394_000839"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCY0498939.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            2675..3706
                     /locus_tag="O0394_000840"
     CDS             2675..3706
                     /locus_tag="O0394_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCY0498940.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGN
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            4198..5604
                     /locus_tag="O0394_000841"
     CDS             4198..5604
                     /locus_tag="O0394_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCY0498941.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVIKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITELMVIEKYEHVMHIVSEVTGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            5601..6167
                     /locus_tag="O0394_000842"
     CDS             5601..6167
                     /locus_tag="O0394_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCY0498942.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTKDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            6173..7171
                     /gene="trpD"
                     /locus_tag="O0394_000843"
     CDS             6173..7171
                     /gene="trpD"
                     /locus_tag="O0394_000843"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HCY0498943.1"
                     /translation="MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAISLNI
                     LNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALEKYHQMRGE"
     gene            7173..7955
                     /gene="trpC"
                     /locus_tag="O0394_000844"
     CDS             7173..7955
                     /gene="trpC"
                     /locus_tag="O0394_000844"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HCY0498944.1"
                     /translation="MTILSEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYDQYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKTNHYYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            7955..8587
                     /locus_tag="O0394_000845"
     CDS             7955..8587
                     /locus_tag="O0394_000845"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HCY0498945.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE"
     gene            8580..9794
                     /gene="trpB"
                     /locus_tag="O0394_000846"
     CDS             8580..9794
                     /gene="trpB"
                     /locus_tag="O0394_000846"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCY0498946.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGDDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEDGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMVERGLAND"
     gene            9787..10515
                     /locus_tag="O0394_000847"
     CDS             9787..10515
                     /locus_tag="O0394_000847"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCY0498947.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIKYLQSIQQTLNN"
     gene            10837..12099
                     /gene="femA"
                     /locus_tag="O0394_000848"
     CDS             10837..12099
                     /gene="femA"
                     /locus_tag="O0394_000848"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HCY0498948.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            12118..13377
                     /gene="femB"
                     /locus_tag="O0394_000849"
     CDS             12118..13377
                     /gene="femB"
                     /locus_tag="O0394_000849"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HCY0498949.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
     gene            14066..14833
                     /locus_tag="O0394_000850"
     CDS             14066..14833
                     /locus_tag="O0394_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0498950.1"
                     /translation="MRFVFDIDGTLCFDGRLIDQTIIDTLLQLQHDGHELIFASARPI
                     RDLLPVLPSVFHQHTLIGANGAMISQQSKISVIKPIHTDTYHHIFKIIQKYELDYIID
                     DDWNYAAQLDAENAIFERLDPHKLASCIDVANIDTPIKIILLNIDPAQITTILDELDK
                     YHQELEMIHHSNEYNIDITAQNINKYTALQYIFDADVKYIAFGNDHNDIVMLQHASSG
                     YIIGPSEAYTHAILKLDKIKHINNNAQAICKVLKSYK"
     gene            complement(14881..15354)
                     /locus_tag="O0394_000851"
     CDS             complement(14881..15354)
                     /locus_tag="O0394_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCY0498951.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGN
                     KVYRCYIDMEHPRKSICNCPHADGRRVICKHMIALLFTASPEAANKHIMMLNEVEEDY
                     QLRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEEEMFEDEFY"
     gene            complement(15478..16179)
                     /locus_tag="O0394_000852"
     CDS             complement(15478..16179)
                     /locus_tag="O0394_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0498952.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQAFSY"
     gene            complement(16172..16945)
                     /locus_tag="O0394_000853"
     CDS             complement(16172..16945)
                     /locus_tag="O0394_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0498953.1"
                     /translation="MSLIDIQNLTIKNTSEKSLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLSCTYDSYQFDGENVSRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKGSKEEALSKVDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            complement(16932..17762)
                     /locus_tag="O0394_000854"
     CDS             complement(16932..17762)
                     /locus_tag="O0394_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0498954.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITLIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH
                     "
     gene            complement(17755..18741)
                     /locus_tag="O0394_000855"
     CDS             complement(17755..18741)
                     /locus_tag="O0394_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0498955.1"
                     /translation="MFIIKSMLYRLMQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKIKTNTPLISESSDRHA"
     gene            complement(18912..19097)
                     /locus_tag="O0394_000856"
     CDS             complement(18912..19097)
                     /locus_tag="O0394_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498956.1"
                     /translation="MKNTAMRALNFAVKIKNNIRQLYAQNHRLLSYTAVTIISKEMEY
                     SAIVVFVLTFLYFTWLT"
     gene            complement(19045..19389)
                     /locus_tag="O0394_000857"
     CDS             complement(19045..19389)
                     /locus_tag="O0394_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498957.1"
                     /translation="MTLFDMPNYLWITTLIMILLTIFCCLVLNKWFVSAVITFVILGV
                     LAFFIPNFQDIKYQPLLGYAAFLAIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYG
                     YEGAELRRKDKK"
     gene            19594..21408
                     /gene="pepF"
                     /locus_tag="O0394_000858"
     CDS             19594..21408
                     /gene="pepF"
                     /locus_tag="O0394_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCY0498958.1"
                     /translation="MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
     gene            complement(21548..22189)
                     /gene="phoU"
                     /locus_tag="O0394_000859"
     CDS             complement(21548..22189)
                     /gene="phoU"
                     /locus_tag="O0394_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCY0498959.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(22196..23047)
                     /locus_tag="O0394_000860"
     CDS             complement(22196..23047)
                     /locus_tag="O0394_000860"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCY0498960.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            complement(23094..24011)
                     /gene="pstA"
                     /locus_tag="O0394_000861"
     CDS             complement(23094..24011)
                     /gene="pstA"
                     /locus_tag="O0394_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCY0498961.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            complement(24013..24939)
                     /gene="pstC"
                     /locus_tag="O0394_000862"
     CDS             complement(24013..24939)
                     /gene="pstC"
                     /locus_tag="O0394_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCY0498962.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            complement(25130..26113)
                     /locus_tag="O0394_000863"
     CDS             complement(25130..26113)
                     /locus_tag="O0394_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCY0498963.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKKFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
BASE COUNT         9285 a         4106 c         4396 g         8548 t
ORIGIN      
        1 aagtaaggga ggaataacag tgtataatta tcatttatta gaagataggg atgttctatg
       61 tattgaccaa aaaagttttt ttgcgagtgt ttcttgtatt gaaaaggggc tagatccatt
      121 agaaacaaag ctagctgttg ttgcagatac taagcgtcag ggttctgtaa tattggctgc
      181 gacacctaaa ttaaaagaat taggcatcaa gacagggtcg cgattgtttg aaataccaca
      241 tagaaatgat atttacatta tcaatccaag tatgcgtaaa tatcttaatg tttcagttgc
      301 tatttctaag attgcattgc gttatattcc acctgaagat ttacaccaat atagtattga
      361 cgaatttttt atggatgtta ctgatagcta tcatagattt agttctacag tacatgcatt
      421 ttgcgaaaga cttaaacgtg aaatttatga agaaacaggc atttattgta ctgtgggcat
      481 tggttctaat atgttattaa gtaaaattgc tatggatgtt gaagcgaagc atagtcaaaa
      541 tggtatagct gaatggcgat atcaagatgt accaacgaaa ttatggccaa ttcagccctt
      601 gcgagatttt tggggtatta atcgtcgaac agaagccaaa ttgaataaaa gaggaatttt
      661 tactatagga gatttagcga aatatccata taaattttta aaaaaagagt tcggtatttt
      721 aggtgttgat atgcatctac atgcgaatgg gatagatcag agtaaagtac gtgaaaagca
      781 caagatcagc aatccatcga tatgcaaaag tcaaatatta atgagagatt atcattttga
      841 tgaagcaaaa gtagtaatgc aagagttaat tgaagatgtt gctagcagag ttcgagcaag
      901 aaaaaaagtg gcaagaacga tacattttgc ctttggctat agtgatgaag gcggtgtaca
      961 taagcaatat actttgaaag atccaacaaa cttagaaaaa gatatttata aagtagtaat
     1021 gcatttcgca gataaattat gtaataaaca agcactatat cgtacgctaa gtatatcttt
     1081 gagtcaattt attaatgagg atgagcgaca gttaagtctg tttgaagatg aataccaacg
     1141 caaacgtgac gaatgtctag ctaaaacgat agaccaatta catttgaaat acggcaaagg
     1201 tattgtgtcc aaagcagtat cgtttacaga agcaggtaca aaacacggca gattaggttt
     1261 aatggctgga cataaaatgt aatgactata cggtttaagt aatatataac tgtgattcgt
     1321 ataaaataag tctctaaaga taaatatttc atatatcaca atagattttc acaataatat
     1381 ctaagaatac atggaattta tcaaaagaga cttaataatt attggatata acaatcaaaa
     1441 tcactcaatg cttgcatacc gcgttctcgg tcagtagggt ttttgaaact aatttttaaa
     1501 gcaccgtata tatcttcgcg tacttctaag attcttaagt tgcttataga tatgttatgt
     1561 aaactcagga tataagtcac tttacttatc atacctgatt catccggaat gtctacatat
     1621 agatcatacg cagtatttag tccacctagt tgtttagcgg gtagtgcgtc gcgatacgat
     1681 ttagcttggg caaaaaatga taacaatttt tcagaatcat tgctttcaat tagtctttct
     1741 aaatcttgaa actgactttt tagctgtcga atcatttcta aaatatacgt tttattactc
     1801 aaggtgatat ctttccacat ttgtgcatta ctactagcta tacgagtgat atcacgaaaa
     1861 ccaccagctg caagtttatt aactaaatga tgttcttgac cgttcttttg actaacatga
     1921 actaaactag atgcaacgat atgaggtaaa tgacttacga cgcttgttac gtagtcgtgt
     1981 tcttcagcag tagttacaat aaatttagca agagtaggtg ataacagttc ttttaacgtg
     2041 tttgctgctt gctcatttct tggctcattg tagactaaaa tataataagc gttttcaaat
     2101 aagtgctttt tagcatttag tacaccagat ttatgactac cagccattgg atgaccactg
     2161 actaaatgaa tattatgctt taataaattg cattcgtgtt gctgtatcat tgctttagta
     2221 ctaccagtat cagaaacaat aacaccaggt ttagttggca tatctataag ctcgctaaga
     2281 tatttatttg tgatagcaac aggtgttgca taaataatta catcggcttt tttaatagct
     2341 tcactataat ttaaacattt ttcattaata atgccgattg atttagcttt atctaactga
     2401 gaagtatctg catcgtatgc aataatatta gtattagggt tatggtattt tatattgcta
     2461 gcaagacttc caccaattaa tccaagccca acaaataaaa ctgttgtcat ataaatcacc
     2521 ttatttcgaa attttcagaa taataacatt gtaaatgagc tgttgacaca gtgcaatagt
     2581 aaataaaaat cgataatagc attaatagat aaacggagat aaatcatcta caataaagag
     2641 tatagtaaca caatggcaac ggaggggtaa atcaatggaa ccaatattag aaatgattaa
     2701 aacattaaca ggtattaata gtccttcagg agacacagaa gaagcaattc aatttgtcga
     2761 aaaatatgca aaagacttgg gttatcaaac aacactaaca aataaaggtg cgttattaat
     2821 aacagtgcca ggcaaaaatg atgaagtaca acgctgtatt actgctcatg ttgatacttt
     2881 aggtgcaatg gttaaagaaa ttaaagaaga tggtcgctta gcaatagaat taattggagg
     2941 attcacgtat aacgcgattg agggtgaata ttgccaaatt aaaactgatg ctggtcaaat
     3001 atatacagga acaatttgtc tgcatgaaac aagtgttcat gtatatagaa ataatcatga
     3061 aatacctaga gatcaaaagc atatggaaat aagaattgat gaagtaacta catcagaaga
     3121 agatacaaag agtttaggta tttcagtagg taattttgtt agctttgatc cacgtacagt
     3181 tatcacgtca tcaggtttta ttaaatctcg tcatttagat gataaagcta gcgtagcgat
     3241 gatactacaa ttactaaaga aattaaaaga agagcaaata atattaccac atacaacgca
     3301 attttatatt tctaataacg aagaaatagg ttacggtgca aatgcatcaa ttgattcgaa
     3361 aatcaaagaa tatattgcat tagatatggg cgcgttggga gacggtcaag catcggatga
     3421 atatacagtt tctatttgtg ccaaagatgc ttcaggtcca tatcataagc aattgaaatc
     3481 gcacctagtt aatctttgca aaataaataa cattccatat aaagtagaca tatatccata
     3541 ttatggttca gatgcttcag cagctttaca tgctggtgcg gatatcagac atggtttatt
     3601 tggcgctggc attgaatcat ctcatgcaat ggaacgaaca catattgatt ctattaaagc
     3661 gacagagaaa ttactatatg catattgctt atcaccaatt gagtaaacaa ttagtgttga
     3721 caaatgtgaa cgacctatgt aatataatga actataaaaa taattagaat tttctaaaga
     3781 aatagtagca gatatgaaac gtagcaaata gaaagctaat gggtgatggg aattagcacg
     3841 ccatatcttg tgaattggac tttggaaaac aattgaatga gttttgaaag tgaacatgaa
     3901 ttatgttaac taaggtggca ccacggtaac gcgtccttac aggtatatgc gttatgtggt
     3961 gtctttttat ttagacaaaa tgtagtagtt aattaaaggt agcaacagaa agttagtgga
     4021 tgatgtgaac taacaccgag attaatgaaa ttgggttttg tctgcaacag aaaaattata
     4081 tatagtaaag agtgaactat gaatatttcg aatattcggt taatttaggt ggtaccacgc
     4141 gtcagcgtcc tttatattga taaggatgct ggcgcttttt tgaaaggagc gtatagaatg
     4201 gatatatttt ataaaaaaat aaaagcaaat gtaacgcccg aagttttagc acaacttcat
     4261 tccaagaaga tcattttgga aagtacaaat caacaacaaa ctaaaggtcg ctattcagtt
     4321 gttatttttg atatttatgg cactttaact ttagataatg atgtattatc agtaagtact
     4381 ttaaaagaat cgtatcaaat cactgaaaga ccgtaccatt atttaacgac taaaataaat
     4441 gaagactacc ataatattca agatgagcaa cttaagtcat taccatttat atctggatat
     4501 gttgggacgt gtagctttga tttagtaaga catgaatttc ctaaattgca atcaatacaa
     4561 ttagaagatc acaagcagca cgatgtaagg ttatatatgg ttgaacaagt ttatgtattt
     4621 gaccattaca aagatgagtt atatatcatc gcgacgaatc aattttcaaa ttcaacaaaa
     4681 tcagatcttg agaatcgagt tattaagtct atcgaagact taactaaaat ccaaccattc
     4741 atgcctacac aagattttga ttttaaaact aaagaaattc aatcaaacat ttctgaagaa
     4801 agatttatcg aaatgattca gtatttcaaa gagaaaataa cagaagggga tatgttccaa
     4861 gttgtgccat caagaattta caaatatgcg catcatgcta gtcagcattt aaatcaactt
     4921 tcgtttcaac tgtatcaaaa tttaaaacga caaaacccaa gtccatatat gtattatctt
     4981 aatatcgatc aaccatatat tgtcggtagt tctcccgaaa gtttcgtaag tgtcaaagat
     5041 caaattgtaa caactaatcc tattgcaggt acgattcaac gtggtgagac gacacaaata
     5101 gataatgaga atatgaaaca actacttaat gatccaaaag aatgcagcga acatcgtatg
     5161 ctagttgatt taggacgtaa tgatattcat agagtaagta aaatcggtac ctcaaaaatt
     5221 actgaattaa tggttattga aaaatatgaa catgttatgc atatcgtaag tgaagtcaca
     5281 ggtaaaataa atcaaaattt atcgccaatg acagttattg cgaatttatt accaacaggt
     5341 accgtttcag gtgcaccaaa attacgtgca attgaaagaa tatatgaaca atatccacat
     5401 aaacggggcg tttatagtgg tggtgttgga tacataaatt gtaatcataa cttagatttt
     5461 gcattagcaa ttcgaacgat gatgatagat gagcagtata tcaacgtaga agctggttgt
     5521 ggcgttgtat atgattctat tcctgaaaaa gaactgaatg aaacgaaatt gaaagctaaa
     5581 agcttattgg aggtgagccc atgatcttag ttgtagataa ttatgattcc tttacatata
     5641 acctagtgga tattgttgct caacatactg acgtcattgt tcaataccct gatgatgata
     5701 atgtgctgaa tcaatcggtg gacgctgtta ttatatctcc tggtccaggg catccattag
     5761 acgatcaaca gttaatgaaa atcatatcaa cctatcaaca caaacccatt ttaggtattt
     5821 gtttaggggc tcaggcactg acttgttact acggtggaga agtcattaaa ggcgacaagg
     5881 ttatgcacgg caaagttgat acactaaagg ttatatcgca tcatcaacat ctgttatatc
     5941 aagatatacc agaacagttt tcaattatga gatatcattc attaataagt aaccctgaca
     6001 attttccaga agaattgaaa attactggac gtaccaaaga ttgtatacag tcattcgagc
     6061 ataaagaaag accgcattat ggtattcagt accatcctga atcatttgct acagactatg
     6121 gtgtcaaaat aattacaaat ttcattaatc tagtgaagga aggatgaaaa ccatgacatt
     6181 actaacaaga ataaaaactg aaactatatt acttgaaagc gacattaaag agctaatcga
     6241 tatacttatt tctcctagta ttggaactga tattaaatat gaattactta gttcctattc
     6301 ggagcgagaa atccaacaac aagaattaac atatattgta cgtagcttaa ttaatacaat
     6361 gtatccacat caaccatgtt atgaaggggc tatgtgtgtg tgcggcacag gtggtgacaa
     6421 gtcaaatagt ttcaacattt caacgactgt tgcttttgtt gtagcaagtg ctggcgtaaa
     6481 agttataaaa catggtaata aaagtattac ctcaaattca ggtagtacgg atttgttaaa
     6541 tcaaatgaac atacaaacaa caactgttga tgatacacct aaccaattaa atgaaaaaga
     6601 ccttgtattc attggtgcaa ctgaatcata tccaatcatg aagtatatgc aaccagttag
     6661 aaaaatgatt ggaaagccta caatattaaa ccttgtgggt ccattaatta atccatatca
     6721 cttaacgtat caaatggtag gcgtctttga tcctacaaag ttaaagttag ttgctaaaac
     6781 gattaaagat ttaggtagaa aacgtgcaat cgttttacat ggtgcaaatg gtatggatga
     6841 agcaacacta tctggtgata atttgatata tgaattgact gaagatggag aaatcaaaaa
     6901 ttacacatta aatgcgactg attatggttt gaaacatgcg ccgaatagtg attttaaagg
     6961 cggttcacct gaagaaaatt tagcaatctc ccttaatatc ttgaatggta aagatcagtc
     7021 aagtcgacgt gatgttgtct tactaaatgc gggtttaagc ctttatgttg cagagaaagt
     7081 ggataccatc gcagaaggca tagaacttgc aactacattg attgataatg gtgaagcatt
     7141 ggaaaaatac catcaaatga gaggtgaata atatgacgat tttatcagaa attgttaaat
     7201 ataaacagtc acttttacaa aatggctatt atcaagacaa acttaatacc ttgaaaagtg
     7261 tgaagattca gaataaaaaa tcttttataa acgcaattga gaaagaacca aagctagcaa
     7321 ttattgcaga aattaaatcg aagagtccta cagttaatga cttacctgaa cgagatttat
     7381 cgcaacaaat ctcagattat gaccaatatg gtgcaaatgc cgtgtccatt ttaactgatg
     7441 aaaagtactt tggtggtagt tttgaaagat tacaagcatt gacgacaaaa acaacattac
     7501 ccgtattatg caaagacttt attatagacc cgcttcaaat tgatgttgct aaacaagctg
     7561 gtgcatctat gattttattg atcgttaaca tcttatctga taaacaattg aaagatttat
     7621 ataactacgc tatatcgcaa aatctagaag tgttagttga agtacatgat cgccatgaat
     7681 tagaacgtgc ctataaggtt aatgctaaat tgattggtgt aaataacagg gacttaaaac
     7741 gatttgttac aaatgtggaa catacaaata ctattttaga aaataaaaaa acaaatcatt
     7801 attatatttc tgaaagtggt attcacgatg catctgatgt aagaaaaatc ttgcatagtg
     7861 gtatcgatgg cttactaata ggtgaggcgc ttatgcgttg tgacaatcta tctgaatttt
     7921 taccacaact gaaaatgcaa aaggtgaagt catgatgaaa ttgaaatttt gtggctttac
     7981 atcaataaag gatgttacag cggccagtca attacctatt gatgcgatag gtttcatcca
     8041 ttatgaaaaa agtaaaaggc atcaaacaat tacccaaata aaaaagttag cgtctgctgt
     8101 tccaaatcat atcgataaag tatgtgtcat ggtaaatcct gatttaacaa caattgaaca
     8161 cgtattaagc aatacgtcaa ttaacacaat acagttacac ggcacagaat ctattgattt
     8221 tatacaggaa attaaaaaga aatattcaag cattaaaatc actaaagctt tagctgcaga
     8281 tgaaaacata atccaaaaca taaataaata taaagggttc gtagatttat ttattatcga
     8341 cacaccctca gtgtcgtatg gtggtaccgg tcaaacatat gactggacta ttttgaagca
     8401 cataaaagac ataccttatt tgatagcagg aggcattaac tctgaaaata ttcaaacagt
     8461 taatcaactt aaattatcac atcaaggtta tgatcttgca tcaggtatag aagtaaatgg
     8521 gcgaaaagat atagaaaaaa tgacagcaat tgtaaatatt gtgaaaggag atagagacaa
     8581 tgaataaaca aatacaaaca gaagcagatg aattaggttt ctttggtgaa tatggagggc
     8641 aatatgttcc agaaacatta atgccagcaa ttattgagtt gaaaaaagct tataaagagg
     8701 caaaagcaga cccagagttt caaagagaac tggaatacta tttatcagag tatgtaggac
     8761 gcgcgacacc acttacatat gctgcatcat atactgaaag cctaggtggc gctaaaatat
     8821 atttgaaacg agaggatcta aatcatacag gcgcccataa aattaataat gcgttaggtc
     8881 aagcgttgct tgctaaaaga atgggcaaga agaagcttgt tgctgaaact ggtgcgggtc
     8941 aacatggtgt agctagtgct acggttgctg cattatttga tatggaactt gttgtcttta
     9001 tgggaagtga agatattaaa agacaacaac ttaatgtatt tagaatggaa ttacttggtg
     9061 caaaggttgt ggcagttgaa gatggtcaag ggactttatc ggatgcagtt aataaagcat
     9121 tgcaatattg ggtaagtcat gtagatgata cacattattt attaggttct gcattaggtc
     9181 cagacccgtt cccaacgatt gttagagatt ttcagagtgt gattggtaaa gaaataaaat
     9241 cacagatatt gaagaaagaa ggtcgacttc cggatgcaat tgtagcatgt atcggtggtg
     9301 gctcaaatgc aatcggtaca ttttatccat ttattaaaga tgatgttgca ttatacggtg
     9361 ttgaagccgc aggtcaaggc gatgatactg ataaacatgc acttgcaatt ggcaaaggat
     9421 cacctggcgt attacatggt actaaaatgt atttaattca agatgaagat gggcaagtgc
     9481 aactagcaca ttctatttca gcaggacttg attatcctgg tattggacca gaacattctt
     9541 attaccacga cattggtaga gtaacttttg aaaatgctag tgatacacaa gcaatgaatg
     9601 ctttaatcaa ctttacaaaa catgaaggta ttatacctgc aattgaaagt gcacatgcac
     9661 tgagttatgt tgaaagacta gcgcctacga tgtcgaaaga agatattatt gtagtaacta
     9721 tttctggacg tggcgataaa gatatggaaa caattagaca atatatggta gagcgaggtc
     9781 ttgcaaatga ctaaattatt tataccttat attatgggca ataaagattt gattgaaaat
     9841 gcaacattgt tgagtgaaaa tggtgcagat ataattgaaa ttggagtacc tttctctgat
     9901 ccggttgctg atggtccagt tatcatggaa gcaggtcaac aagcgattaa acaaggcatc
     9961 acgatagatt atattttcaa tcaattagaa aaacatggtg atcaaattaa gtgtaactat
    10021 gtattaatga cgtattataa tattatttgt cattatggag aacaagcgtt ttttgaaaaa
    10081 tgtcgagata ctggtgtcta cggcttaatt attcctgatt taccatatga attatcgcag
    10141 cgtttaaaac aacaatttag tcactatggc gtcaaaatca tatcgttagt tgcgatgact
    10201 actgatgaca aacgtataaa agatatcgta tcccatgcgg aaggctttat ttatactgtg
    10261 acgatgaatg cgacaacagg gcaaaacggt gcgtttcatc cagaattaaa acgaaaaatt
    10321 gagtcaatta aagcgatagc caatgtgcca gttgtcgcag gatttggtat aagaacacca
    10381 caacatgttg cagatataaa agaggttgca gatggcattg tcattggtag cgaaatcgtt
    10441 aagcgattta aatctaacac gcgtgaggaa atcattaaat atttacaatc tatccaacaa
    10501 acattgaata attaagttta cttgatttaa aaaaattagg cgaatactgt ttgaaaaagt
    10561 gaaaaacggt gaattataaa attgaataga aatgatacaa aagtaatatg agcaaaccca
    10621 aacgttcata ttactttttt tgaaattgta ttcaaaaatc taaatattac tataaaagta
    10681 tacgcaatta aagcgtttat gttttagttt taacattaac tattgtatac ttatttagat
    10741 tagatttatt atttttgaca tttgcagagg ggaaatagaa aaactgcaaa tacggaaatg
    10801 aaattaatta acgagagaca aataggagta atgataatga agtttacaaa tttaacagct
    10861 aaagagtttg gtgcctttac agatagcatg ccatacagtc atttcacgca aactgttggc
    10921 cactatgagt taaagcttgc tgaaggttat gaaacacatt tagtgggaat aaaaaacaat
    10981 aataacgagg tcattgcagc ttgcttactt actgctgtac ctgttatgaa agtgttcaag
    11041 tatttttatt caaatcgcgg tccagtgatt gattatgaaa atcaagaact cgtacacttt
    11101 ttctttaatg aattatcaaa atatgttaaa aaacatcgtt gtctatacct acatatcgat
    11161 ccatatttac catatcaata cttgaatcat gatggcgaga ttacaggtaa tgctggtaat
    11221 gattggttct ttgataaaat gagtaactta ggatttgaac atactggatt ccataaagga
    11281 tttgatcctg tgctacaaat tcgttatcac tcagtgttag atttaaaaga taaaacagca
    11341 gatgacatca ttaaaaatat ggatggactt agaaaaagaa acacgaaaaa agttaaaaag
    11401 aatggtgtta aagtaagatt tttatctgaa gaagaactac caatttttag atcatttatg
    11461 gaagatacgt cagaatcaaa agcttttgct gatcgtgatg acaaatttta ctacaatcgc
    11521 ttaaaatatt acaaagaccg tgtgttagta cctttagcgt atatcaactt tgatgaatat
    11581 attaaagaac taaacgaaga gcgtgatatt ttaaataaag atttaaataa agcgttaaag
    11641 gatattgaaa aacgtcctga aaataaaaaa gcacataaca agcgagataa cttacaacaa
    11701 caacttgatg caaatgagca aaagattgaa gaaggtaaac gtctacaaga agaacatggt
    11761 aatgaattac ctatctctgc tggtttcttc tttatcaatc catttgaagt tgtttattat
    11821 gctggtggta catcaaatgc attccgtcat tttgccggaa gttatgcagt gcaatgggaa
    11881 atgattaatt atgcattaaa tcatggcatt gaccgttata atttctatgg tgttagtggt
    11941 aaatttacag aagatgctga agatgctggt gtagttaaat tcaaaaaagg ttacaatgct
    12001 gaaattattg aatatgttgg tgactttatt aaaccaatta ataaacctgt ttacgcagca
    12061 tataccgcac ttaaaaaagt taaagacaga attttttagg aagggaatta tcaaaacatg
    12121 aaatttacag agttaactgt taccgaattt gacaactttg tacaaaatcc atcattggaa
    12181 agtcattatt tccaagtaaa agaaaatata gttacccgtg agaatgatgg ctttgaagta
    12241 gttttattag gtattaaaga cgacaataac aaagtaattg cagcaagcct tttctctaaa
    12301 attcctacta tgggaagtta tgtttactat tcgaatcgtg gtccagtaat ggatttttca
    12361 gatttaggat tagttgatta ttatttaaaa gagttagata aatatttaca gcaacatcaa
    12421 tgtttatatg ttaaattaga tccgtattgg ttatatcatc tatatgataa agatatcgtg
    12481 ccatttgaag gtcgcgagaa aaatgatgcc ctagtaaact tgtttaaatc acatggttac
    12541 gagcatcatg gctttacaac tgagtatgat acatcgagcc aagtacgatg gatgggcgta
    12601 ttaaaccttg aaggtaaaac acccgaaaca ttgaaaaaga catttgatag tcaacgtaaa
    12661 cgtaatatta ataaagcgat aaactatggt gttaaagtca gattccttga acgtgatgag
    12721 ttcaatcttt tcttagattt atatcgtgaa actgaagagc gtgctggatt tgtatcaaaa
    12781 acagatgatt atttttataa ctttattgac acatatggag ataaagtatt agtaccatta
    12841 gcatatattg accttgatga atatgtgtta aagttgcaac aggaattgaa tgacaaagaa
    12901 aatcgtcgtg atcaaatgat ggcgaaagaa aacaaatcag ataagcaaat gaagaaaatt
    12961 gcagaattag ataagcaaat tgatcatgat cagcatgaat tattgaatgc aagtgaattg
    13021 agcaaaacgg acggcccaat tctaaacctt gcttctggcg tttattttgc aaatgcatat
    13081 gaagtgaatt atttctctgg tggttcatca gaaaaatata atcaatttat gggaccatac
    13141 atgatgcatt ggtttatgat taactattgc ttcgataatg gctatgatcg ttataatttc
    13201 tatggtttat caggtgattt tacggaaaac agtgaagatt atggcgtata ccgctttaaa
    13261 cgtggattta atgtacaaat cgaagaatta ataggggatt tctataaacc aattcataaa
    13321 gtgaaatatt ggttgttcac aacattggat aaattacgta aaaaattaaa gaaatagatg
    13381 taaatgatgt tagggcttat agttattgat actataggct cttttttata tgttttttaa
    13441 taaagcctcg aataatgata tattaaatga caagtttaac aagtgattgc aataaatata
    13501 aatatgaatt gcacaaccta tttgtaaatg attgagttga gtatactttt taattcattc
    13561 aatatgactc aaaacaagcc aatatgtatt atatttttgg tgaataaacc ttagcataac
    13621 aatttatgtc tgattatata tgtgtgtgtt tgcaagtgtg tgatatcaaa tgtcatattt
    13681 gtctaattgt aatttataaa atgcatgagc aatgattgct gtaacaaatt ctagcaaagt
    13741 attcagtttg tattttagaa ataaaaatga tgatagatgt atgatcgata taaaagattt
    13801 aatatgcacc aaaatgataa cgtgagtaat tggtttaatg aaacataaca tgaatgtaaa
    13861 tttagcttac tgacataaac actacacaaa catacagtta tataagtccc cttatggaat
    13921 ttcacattct agtttacata atatatatta taggaagtta tatgtgtgta acgcaaaagg
    13981 taccctacat cataatcatt atctaatatc gtcacataac ttacttatgc tataatcatg
    14041 gtattatatt gtttggagtg atttgatgag atttgtcttt gatattgatg gtacgctttg
    14101 ttttgacggc cgattaattg accagactat tattgataca ttgttacaat tacaacatga
    14161 tggtcatgaa cttatatttg catcagcacg tccgattcgt gatttgttgc cagttttacc
    14221 atcagtattt catcagcaca cattaattgg cgcaaatggt gctatgattt cacagcaatc
    14281 aaagatttct gttatcaaac caattcatac tgatacatat catcatatct tcaaaataat
    14341 tcaaaagtat gagttagatt atattattga tgatgattgg aattatgctg cacaacttga
    14401 cgctgagaac gcgatttttg agcgtttaga tccacataag ctggccagtt gtattgatgt
    14461 tgcaaatatc gacacaccaa tcaagattat tttattaaat atagacccgg cacaaattac
    14521 aactatatta gacgagctag ataaatacca tcaagaattg gaaatgattc accattcaaa
    14581 tgagtataac attgatataa cagcgcaaaa tattaacaaa tatactgcat tacaatatat
    14641 atttgatgca gatgttaaat atatagcatt tggtaatgac cacaatgata ttgtcatgtt
    14701 acaacatgct agtagtggct atattatagg accatcagaa gcatacacac acgcaatatt
    14761 gaaacttgat aaaatcaaac acatcaataa taatgcacaa gctatttgca aagtcttaaa
    14821 atcatataaa taaaaacacc cctatcaaat gataatcatt atcaatcgat aggggctatt
    14881 ttaataaaat tcgtcctcga acatttcttc ctcttcatct aatccaaata attctgccat
    14941 ttctccatgt tcaattaaca tgtttaaata tgcatcgcgg agttcttctt cactcatatc
    15001 attaatcatt tctttaagac tatcaatcca catatttctg cgtaattgat agtcttcttc
    15061 aacttcgttt aacatcatta tatgtttatt tgctgcttct ggactagctg taaagagtaa
    15121 tgcaatcata tgtttacata tcactcgtct tccatcagca tgaggacaat tacatatgga
    15181 ttttctagga tgttccatat caatataaca acgatatact ttgttgccac tgccctttac
    15241 ttcagcctca tgctgcgttt ctgaaaatga ttttaagtta atgacgcatt cactttgata
    15301 ataattaaag cctctttcta tagaacgaat acttgcaata tcaagtaatc ccattaatga
    15361 tactcctttt tattattatt tttaaataaa gaaaataaaa tagataagtg tctagattaa
    15421 aatacttgat ttatctatat tttataacaa gtctagaatt atcgcattct taaataacta
    15481 atatgaaaat gcttgcacta attcttttgt ataagggtgt ctatcaacat taaataattc
    15541 ctctattgca aaatcatcga ctatcatgcc atccttaaga acgataattc tattaactaa
    15601 gcgttgtaac acggataaat catgagaaat aacgataaaa tgatttaagt tcgtaatcgt
    15661 ttgcgctttt aatatattga ttacattttg ttcagctata acatctaaat ttgaagttat
    15721 ctcatcacat attaaaacgc gaggctgtgc taataacgaa cgcatgacat taaatctttg
    15781 taattgtccg ccactcactt cgcttggtaa tttagtcaat aattgcgcgt ttaactcaaa
    15841 agtagataaa tgttgtaata ataattgatc ctgagcagta ttatcagtta gacctctgta
    15901 ataatataac gcttctttta atgaggtctc aatcgtccaa tcagggttaa agctagttaa
    15961 agggtgttgg aaaatcggta acacagcatt gtcacttaag taaatctctc ctttaacagg
    16021 tttaaacaag ccaagaacca atgaagcgag cgtactttta ccacagccac tttcgcctaa
    16081 aataccaaca ttttctccat caggtatagt aatattgata tcttgtagca ccatctgctt
    16141 tttattataa ccaaaagtca catgttttaa ctcaatcata gtaatccctc tttaattgtg
    16201 ttctatattt aattagacgt tcagtatacg gatgcaaatg ctcatacttg aaatgattaa
    16261 tattacctcg ttcaatgatt tgaccttctt ttaaaacata aatgtactga caatatttca
    16321 atacatgact taagttatgt gtgataataa ataatgtttg accatgttct aatacaatat
    16381 gctgtaataa atccatcact tgattaccgt tcaaagcatc caatgatgca actggttcgt
    16441 ctgcaatgat taatttaggc tccaacatga gaacgcttgc tatgtatacg cgttcaagtt
    16501 ggcccccaga aagttggaaa ctatatttat ttaatatatc tttgctttgt aaattaaccc
    16561 acgacaaagc cttatcaact ttggacaaag cctcttcttt actaccttta taatgcttac
    16621 gataaatcgc agttaactgt ttacctaatt tagtatggtc gttaaaactt tctgcataat
    16681 tttgagaaat atagccaatt gtatgaccat aatattgact caatctacta acattttccc
    16741 catcaaattg gtacgaatca tacgtgcagc ttaaatcaaa tggtaaatat tcaagtaaag
    16801 ctttagcaat caaacttttt ccagcgccgc tctctccaat caaggcatta atctgttgac
    16861 taaaaatttt caaatcaatc cctttaataa gagatttctc actagtattc tttattgtta
    16921 aattttgtat atcaatgaga ctcatcatat tcaccccgtt gtttcagcaa tctatctctt
    16981 agtgcatcac cggttaaatt aaaaattaaa atagttatag caatgactga agcaggtgca
    17041 atcaacataa ttggatgaga cgaaataaaa tcacgacctt gttgcaacat agcgccccac
    17101 tctggtgttg gcggttgtgc acctaaccca ataaatgata gtgaacttat atatagaatg
    17161 attttaccga aatcaacgac catcaaaacg ataatagccg gtataatttt aggtgttaaa
    17221 tgacgtatta atattgttct tgttggtaca tgaaataatt gtgccatttt tatataaggc
    17281 ttattcattt cgctattaac tatacttcta gtcaaccttg tgtaattcat ccattttatt
    17341 aatgtaattg agataactaa attccataaa gatggttgaa aaaaacttgc taaagcaatc
    17401 atgatgataa attctggaat acttagacca acatcaataa accttaacac taatcgttca
    17461 atccaccctt ttttgtatcc ggcaaataga cctagtgtaa cacctatgac aacgatagct
    17521 attaatgtta aaacagtaac aaacaatgtt gaacgtgcac cgataataat tcgggtaaat
    17581 aaatctctcc cataatcatc agttcctaat aaatgcaacc aactaatagg ttcaaaagtt
    17641 tgtgataaat tgactttggt tgcattttca ctactgacaa agaattgcag tacaattacc
    17701 acaaaaataa atgcaacgaa tacaaaaaat atcaggttat tctttgaaaa tattttatgc
    17761 atgacggtca ctactttctg atatcaatgg tgtattggtt ttgatttttg gatttcctaa
    17821 ttgtaaacgc tgcttcggat caagtaataa cgttaataaa tcagcaatcg tattgataat
    17881 aacaacgaag aagccaataa ataacacgca tccttgaata acaggataat ctcgagattt
    17941 aatactatcc attaatagat aaccaatacc aggtatatca aataaatttt caatcactac
    18001 agtaccacct attagactgc caagtgaaat ccctagtaat gggataatcg gcaaaattgt
    18061 tggttttagt aaatcatgaa ttaaaatata acgttcattc ataccgcgta atcttgatgc
    18121 ttgtacgata ttactttgca ataacatcaa taaattagaa cgcactaaac gaatgatgta
    18181 tgcacacata cctaaagata gcgtgattac aggtaatata aactgactta gtataacgct
    18241 atctatattc attaaatttg tgacaataaa taataaaata ataccgataa agaacgctgg
    18301 taaactaatc gatagtgttg agatcactct aatcacttta tccgtccact tatgaaatcg
    18361 tttggctgct ataatgccga gcggtataga tatgcataac gacactacta atgttgaaaa
    18421 tgatatgagt aatgttatgg gtgcatagtt gaataatatc tgtgttaccg gttcttttga
    18481 ttcaaaactt tttcctaaat taaaatgtaa taaatgattc atccaatgcc accactgtac
    18541 caataaagaa tcatttaatc ccaatttatc tttggttgca tttatttgtt ccgtcgacac
    18601 ttgtgctaca tcaagatgta atattttatc aacaggattg cctggtgata atttcattaa
    18661 aatgaatgta agtgtagaaa taacaaataa aacaactatc atttgcatca gtctatacaa
    18721 catagacttt attatgaaca taatagtccc cctccttgtg taagttacta acactttctt
    18781 tttacatgag aatggcgcat gtatatgcaa cttacatatt aagaactaac gttcattata
    18841 gtattatcca taaagaaatt gaagtatatt taatttttta acaaaatcat tataaaatat
    18901 aatattttga atcaagtcaa ccatgtaaaa tataaaaaag tcaaaacaaa aacaactata
    18961 gcactgtatt ccatctcttt cgaaataatt gttactgcag tgtaacttaa aagtcgatga
    19021 ttttgtgcat atagttgtcg aatattattt tttatcttta cggcgaagtt cagcgccctc
    19081 atagccgtat ttttcaattt gcttttctaa tttacgcgct tttctttctt tacgccaatt
    19141 tctagtaaaa taccataata gaaaactaat taataaactc ataatcgcta aaaatgcagc
    19201 gtatcctaat aatggttgat attttatatc ttgaaaattt ggaataaaaa atgcaagcac
    19261 acctaatata acaaatgtaa ttactgcaga tacaaaccat ttatttaaaa ctaagcaaca
    19321 gaatattgtt aataaaatca ttattaatgt tgtgatccat aaataattag gcatatcgaa
    19381 taatgtcata ttcattctcc ttttatttca ttactttcct tgtatacatt ttattataaa
    19441 tttttaaaaa cttaaacaat agcagtcagt ttcaagcaat attctatcta ctaatagaaa
    19501 aatcattgtt ccttgcgaca tggaaatcgt aacattatcg tttaggagac aaaattatgt
    19561 ataatgaatg tattatacca aaggagtgat tatatgtctc aaggtttacc tttaagagaa
    19621 gatgttcctg tttcagaaac atgggattta gtagacttat ttaaagatga tcaacaatat
    19681 tatgaaagta ttgacgctct agtacaacaa gcaaatcaat ttcatcatac atatgcaaca
    19741 acattaaatt caatcgaaca aattaatact gctttagctg aattagaaaa tattttaatt
    19801 gccttagatc gcttaagtaa ttatgcagaa ctacgtttaa gtgtagatac tagtaatatc
    19861 gaggcacaag tattgagcgc taaattatct actacatacg gtaaaattgt tagccaatta
    19921 tcatttgtag agtcagaaat acttgaatta ccagaagaaa tacttcaaca attagaagaa
    19981 tcatgtccat atcaacacta tattaaacag ttaataaaac aaaagccatt ccaattatct
    20041 gcgtcggtag aacaagtatt agcaacttta tcacctacgc taaacagtcc ttacgattta
    20101 tacggcacga caaaaatgct agatattaca ttcgattcat ttgaacatga tggtacaacg
    20161 taccctgtcg actatgctac gtttgaaaat gattatgaag ataataaaga tcctgagttt
    20221 agacgtaaaa gtttcaaatc gtttagcgat gggattcgaa aatatcagca tactaccgcg
    20281 gctacatata atatgcaagt acaacaagaa aaaattgaag ctgatttacg tggatttgaa
    20341 tcagtcatcg attatttatt acatagtcaa gaagtaacgc gtgatatgtt tgaccgtcaa
    20401 atcgatatga ttatgcgtga cttggcacca gttatgcaga aatatgctaa acttttacaa
    20461 cgtattcacg gattagataa catgcgtttt gaagacttga agatttctgt agaccctgat
    20521 tatgaaccag agatttcaat tgaagactca aaaaattata ttttcggtgc gttaagtgtt
    20581 ttaggtgatg actatacaaa catgttacgt gaagcatacg atcagcgatg gattgatttt
    20641 gcacaaaata aaggtaaaga tacaggcgca ttttgtgcaa gtccatactt tacacattca
    20701 tatgtgttta tttcttggac tggtaaaatg gctgaagcat ttgtcttagc acatgaatta
    20761 ggtcatgcag gtcattttac attagctcaa aaacatcaac catatcttga atcagaagca
    20821 tcaatgtact ttgttgaagc cccttctaca atgaatgaaa tgttgatggc caattattta
    20881 tttaacacaa gtgataatcc aagatttaag cgttgggtta ttggctcaat tttatctaga
    20941 acatattatc ataatatggt tacccattta ttagaagctg cttatcaacg tgaagtgtat
    21001 cacaaagtag atcaaggtga atctttaaat gcgccgacat taaatgaaat aatgctaaat
    21061 gtttataaac aattttttgg agatgcagta gacatgactg agggtgctga attaacatgg
    21121 atgcgtcaac ctcattacta tatgggatta tattcgtata cgtattctgc tggcttaaca
    21181 atcggaactg tcgtttctca aaagattaaa aatgaaggcc aaccagctgt tgatgcttgg
    21241 ttagaaacat tgaaaaaagg tggtagtgta tcacctgtcg aacttgcaaa cattgcaggt
    21301 gtagacatta ctacagaaca gccacttaaa tctacaattc aatatatttc tgatttagtc
    21361 gatgaagttg aaaaattaac agatgaaatt gagcaagcaa ataactaaga tgcgagacaa
    21421 tttatagtag aacaatataa accaagattt tcacattact aattgtgaga atcttggttt
    21481 ttttgagggt ttatgaataa tagaaatata tatttatcgt aagggatttt atagtaataa
    21541 ctttaagtta ttgttcgtaa tgtgtacctg ttaaataaaa ataaacactt tcagcgatgt
    21601 taataatatg atcaccaata cgttctaaat gtcttgctgc taaatgagct tgtgcagcga
    21661 caaatggatc gttatcaata agatacgttg cgttaataat atgactatat aagtcatcga
    21721 tatcttcatc acgctcaatt atttctctta ttaatacggt atctttcttt ttaaatgctt
    21781 gatctaagtc ctttaacatt aacatagcta atttacccat tgtctttaaa cgggttaaca
    21841 cataatcatc tgtaatcttt gtacgcaatc gaatattggc aatactcgag gcattatctc
    21901 ctattctttc taaatcggag gcgattttta atgaagaaat catcatacgc aaatcactcg
    21961 caatgggctg ttgctttgta attaacatga taactcgctc attaatatca taatttaatt
    22021 gattgatatg tttatcgttt ttaactgttt gtcgtgcaaa gcctctatcg tcaatactta
    22081 atgattttat accattttca atactcacat agacatttgc acctaaccga cgtaattctt
    22141 ttattaaatc atcaagttgc tcctgatatc gttgtctaat tattgccatt atatatcaac
    22201 caaaccttcc tgaaatataa tcttctgttt tcttgtttga tgggttagag aaaattttat
    22261 cagtatcatc atattcattg acataaccat ttaagaaaaa tgcagtttta tctgatacac
    22321 gagctgcttg ttgcatatta tgtgtaacca taataattgt atacttttct tttagttctt
    22381 gaaccaactc ttctactctt aatgttgaga ttggatctaa tgctgatgtc ggttcatcca
    22441 ttaaaatgac ttcaggttca attgctaaac aacgcgcgat acaaacacgt tgttgttgcc
    22501 caccggataa actatatgca tttgtgtgca acctatcctt taattcatcc caaattgcag
    22561 cgccacgtaa tgatttctca acgatttcat caagaacttt tttattttta ataccgtgaa
    22621 tctttggacc gtaagtaata ttatcgtata ttgattttgg aaatggatta ggttgttgaa
    22681 agaccatgcc cacatttgta cgtaattgtt ctttagaata tttttgatca aaaatgtctt
    22741 gatctcgata taatatttta ccagctgttt tcacagaagg tactaactca accattcgat
    22801 tcaaagtttt aatatatgtt gatttgccac aaccagatgg acctataatg gcagtaattt
    22861 ggttttcata aatatctaaa ttaatatttt gtaatgcatg attttcgcca taccataagt
    22921 ctaaattttg tgttgaatat ataacagaat gtgagtttgt atcatctggt gttttatgat
    22981 gactttgtga gacatcaaac gtatgacttt gagatatttg tttagtttgt gcaagtgttt
    23041 gcgccatatt taaaactccc tttttaatag ataaatgaga ttgattgttt aaattagaat
    23101 tttttactaa atttgttacg taaaataatc gcaacgccat tcattaagat taagataact
    23161 agtaaaacga taatgcctgc cgatgcaaca ttctggaatt cttcttgagg cattttcgcc
    23221 caagtaaata tttggatagg taatgctgaa aattgatcca atatacttct aggtgttgcc
    23281 aataatatag tcggtatacc gattagcaca agtggcgctg tttctcccag tgctcttgaa
    23341 agagacaaaa tgaatccagt taaaatacca ggtaacgctg ctggtaagac aacacgtctt
    23401 atcgtttgcc atttattagc acctaagccg taagaagctt cgcgtactga gttaggtaca
    23461 gctctaattg cttcctgact tgaaacaata ataattggta atatcagtaa ggtcattgtt
    23521 agcgctgctg ccaatatact gttacccatt ttcaaggctt caatccctgc accaccaacg
    23581 aacaaagtat aacctaataa cccaaataca actgatggta caccagctaa attggaaata
    23641 ctgattttaa caaactgagt aaatttgttg tttttcgcat attcttctaa gtatatagct
    23701 gtacctattc ctaggatgat tgataatgga atgatactta acattaacca aagtgaaccg
    23761 attaacgcgc ctttaacgcc agccatagat ggtgttgaag aagaaaaatt agtgaaaaac
    23821 tgtaaattta aatgacttac ccctttaatc aatgtttgag ttaacaacgc aataagtacg
    23881 acaagtccta ataatgtaca tgctaaaaat atgagtttga acactttatt tttaaccgtt
    23941 ctggatgata aatgtttttg gacaagttgt tgatcgacga gtgattgtct attattatct
    24001 gtcgtttcca tattaatact cctccctaaa acgcttagaa atccactgag aaagtaaatt
    24061 catgattaag gtaaagataa atagtgtgaa ccctacagca taaatactgt aataaatatt
    24121 tgatccaaat gttgcatcac ctgtcgctat ctcaacaata tatccagtca ttgtttgaat
    24181 cgaacttgtt aaacttaatg aagctgttgg cgaactacct gccgctaatg atacaatcat
    24241 cgtttctcca attgctcttg aaatcgcgag aacgattgaa gctacaatac ctgatgttgc
    24301 tgcgggaagt acgactttag ttgctacttc taatttagtt gctccaagtc cataggcacc
    24361 ttctcgaatt ttatttggta cagatgccat tgcatcctca ctcaaacttg tgatgagagg
    24421 gacaatcata ataccgacaa ctaagccggg acttatagca ttaaactctc caagacctgg
    24481 tatgaaagat cttaatactg gtgtaacaaa ggttaatgca aagaaaccaa acacaattgt
    24541 tggtattcct gctaaaattt ctaatatcgg tttaattatg cgtcgtgcac ggtcacttgc
    24601 atattcactt aaataaattg ctgcaccaag cccgactgga actgcaaata tagtcgcaat
    24661 aactgtgatt tttaaagtcc ctattatcaa tgcccagata ccaaacttag ggtctgaacc
    24721 ggtaggattc caagtagtag aaaatagaaa ttcagttatt ggaattctgg tgaaaaaagt
    24781 gatggtttct aaaagcaatg tgattaatat acctagtgtt gttaaaatgg aaatcgctga
    24841 aattgcggct aaaataactg gtataatttt gtcattatgc tttccttttt tattattatt
    24901 tttttcgatt aaagctttaa cattagtaga tgaagtcata atcaaacctc tttctattac
    24961 tcccaaaata attagatttg taaaataatg atgaaccact attttaagtt gataagcttg
    25021 tagactaact gaatgtcaaa atcagcgtct cgataaatca agctttgtac tctaaataat
    25081 gattcaccat caaaatcatg tttcaagaca catttgaaat tgcgtcttat tattttttgt
    25141 cttcagactt tttatcatca gatttcttgt cgtctgattt ttgattttta tcaataaatg
    25201 cttttaaatc atctaattgt gatttgtatg ttttctctgg tgctgctaca tatccagctt
    25261 cttcagctgc tttaccttta tcttctaaga cgaatttgat aaattctgac attactttat
    25321 tatctttcaa tgctttttca tttacataaa tgaataatgg tctacttaat gcataagagt
    25381 tatcttgaat tgtttttttc gtaggctctg ttgctttacc attttcatct ttgattttaa
    25441 cttcttttaa tttatcttta ttttgtacgt agaagttata tccaaagtat ccgattccct
    25501 ctttgttttt cgttacagaa gaaacgatag catttgtatc agcatttttt tctgctttaa
    25561 tatcttcttt attcattact tcattttcaa agaagtcata agtaccatga cttgagtttg
    25621 gtgatacagc atttattttt ttatctggcc atttactatt aacatctttc catgttttag
    25681 cttttccaga ataaattgct tttaattgct gtttgtctaa ttcatctaca aaatcatttt
    25741 ctttatttac agcaaccgtt acaccatctt gcgcaatttt gaattttttg tatttgatat
    25801 tcttatcttg taatttttgc ttctcttcat ctttaattgg tctagaagca tcagcgaagt
    25861 cgatatctcc tgcaatgaat ttttggaaac cagcacctgt accagcttgt cctgctgaga
    25921 ttttagcatc cgagtgatct tgagcccatt tttcatttaa tttctccaca attggtgcta
    25981 ctgttgatga gccatcacct ttagcttccc cttttaaatc actattacca ctgccaccat
    26041 taccgccacc acaagcacct aataatagtg ttgcacctaa agctgtagta ccaacaaatt
    26101 gccatttttt cattgaaata tcctccctgt atgaacaaca aatagtattt gtattgatta
    26161 catctttatc ttaaccttga attattaacc tcatttttag aaactgtaaa tgttacataa
    26221 aaaattgtta agaaaatgta aatattaatt tttgttaatc taaaaaatat tgcttcatta
    26281 gtaaaattaa aatgcttcct gaaaaatcat cgaaatttaa atatgaatac aaaaa
//
LOCUS       DAKTLH010000024        25548 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.024, whole genome shotgun sequence.
ACCESSION   DAKTLH010000024 DAKTLH010000000
VERSION     DAKTLH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25548
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.024"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            248..1633
                     /gene="fumC"
                     /locus_tag="O0394_000864"
     CDS             248..1633
                     /gene="fumC"
                     /locus_tag="O0394_000864"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCY0498964.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAAIANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(2096..2917)
                     /locus_tag="O0394_000865"
     CDS             complement(2096..2917)
                     /locus_tag="O0394_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCY0498965.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTIDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKEAVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            3080..4192
                     /locus_tag="O0394_000866"
     CDS             3080..4192
                     /locus_tag="O0394_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HCY0498966.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            4214..4837
                     /locus_tag="O0394_000867"
     CDS             4214..4837
                     /locus_tag="O0394_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0498967.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
     gene            5193..5657
                     /locus_tag="O0394_000868"
     CDS             5193..5657
                     /locus_tag="O0394_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0498968.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            5836..6960
                     /locus_tag="O0394_000869"
     CDS             5836..6960
                     /locus_tag="O0394_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCY0498969.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLNQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASKQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            7029..7373
                     /locus_tag="O0394_000870"
     CDS             7029..7373
                     /locus_tag="O0394_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HCY0498970.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            7756..7854
                     /locus_tag="O0394_000871"
     CDS             7756..7854
                     /locus_tag="O0394_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498971.1"
                     /translation="MPFDSLAVLITTEIAFPTEIFEKYQRTLQIKF"
     gene            8145..9341
                     /locus_tag="O0394_000872"
     CDS             8145..9341
                     /locus_tag="O0394_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCY0498972.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            9331..12267
                     /locus_tag="O0394_000873"
     CDS             9331..12267
                     /locus_tag="O0394_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCY0498973.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQSNIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            12264..13205
                     /gene="yhaM"
                     /locus_tag="O0394_000874"
     CDS             12264..13205
                     /gene="yhaM"
                     /locus_tag="O0394_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HCY0498974.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(13326..14288)
                     /locus_tag="O0394_000875"
     CDS             complement(13326..14288)
                     /locus_tag="O0394_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HCY0498975.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
     gene            complement(14493..15050)
                     /locus_tag="O0394_000876"
     CDS             complement(14493..15050)
                     /locus_tag="O0394_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCY0498976.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLALNFGISITDLLYLKIIIFS
                     NYGQYIEEHSTGINILKKIKNPYHL"
     gene            complement(15783..16148)
                     /locus_tag="O0394_000877"
     CDS             complement(15783..16148)
                     /locus_tag="O0394_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCY0498977.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            complement(16290..16712)
                     /locus_tag="O0394_000878"
     CDS             complement(16290..16712)
                     /locus_tag="O0394_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCY0498978.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            16846..17586
                     /locus_tag="O0394_000879"
     CDS             16846..17586
                     /locus_tag="O0394_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0498979.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDETMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            17579..18802
                     /locus_tag="O0394_000880"
     CDS             17579..18802
                     /locus_tag="O0394_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0498980.1"
                     /translation="MRNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFSLIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFITFALTYYLLLAPKWYIAFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(18926..19429)
                     /gene="traP"
                     /locus_tag="O0394_000881"
     CDS             complement(18926..19429)
                     /gene="traP"
                     /locus_tag="O0394_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HCY0498981.1"
                     /translation="MKKLYTSYGTYGFLHQIKINNPTHQLFQFSASDTSVIFEETDGE
                     TVLKSPSIYEVIKEIGEFSEHHFYCAIFIPSTEDHAYQLEKKLISVDDNFRNFGGFKS
                     YRLLRPAKGTTYKIYFGFADRHAYEDFKQSDAFNDHFSKDALSHYFGSSGQHSSYFER
                     YLYPIKE"
     gene            19592..19702
                     /locus_tag="O0394_000882"
     CDS             19592..19702
                     /locus_tag="O0394_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498982.1"
                     /translation="MIKCQFYDIYLLKIRTNERYDNIDKNDFNLGGLYGA"
     gene            19692..20729
                     /gene="hemE"
                     /locus_tag="O0394_000883"
     CDS             19692..20729
                     /gene="hemE"
                     /locus_tag="O0394_000883"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCY0498983.1"
                     /translation="MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            20787..21710
                     /gene="hemH"
                     /locus_tag="O0394_000884"
     CDS             20787..21710
                     /gene="hemH"
                     /locus_tag="O0394_000884"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCY0498984.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            21734..23134
                     /locus_tag="O0394_000885"
     CDS             21734..23134
                     /locus_tag="O0394_000885"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HCY0498985.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGFDLLKKPTQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYNDQLEEVY
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTHDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            23457..24011
                     /locus_tag="O0394_000886"
     CDS             23457..24011
                     /locus_tag="O0394_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HCY0498986.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
     gene            24483..24556
                     /locus_tag="O0394_000887"
     tRNA            24483..24556
                     /locus_tag="O0394_000887"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24517..24519,aa:Met,seq:cat)
     gene            24578..24653
                     /locus_tag="O0394_000888"
     tRNA            24578..24653
                     /locus_tag="O0394_000888"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24611..24613,aa:Asp,seq:gtc)
     gene            24670..24742
                     /locus_tag="O0394_000889"
     tRNA            24670..24742
                     /locus_tag="O0394_000889"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24703..24705,aa:Phe,seq:gaa)
     gene            24756..24831
                     /locus_tag="O0394_000890"
     tRNA            24756..24831
                     /locus_tag="O0394_000890"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24789..24791,aa:His,seq:gtg)
     gene            24847..24920
                     /locus_tag="O0394_000891"
     tRNA            24847..24920
                     /locus_tag="O0394_000891"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24879..24881,aa:Gly,seq:tcc)
     gene            24935..25009
                     /locus_tag="O0394_000892"
     tRNA            24935..25009
                     /locus_tag="O0394_000892"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24967..24969,aa:Asn,seq:gtt)
     gene            25011..25082
                     /locus_tag="O0394_000893"
     tRNA            25011..25082
                     /locus_tag="O0394_000893"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25044..25046,aa:Glu,seq:ttc)
     gene            25137..25225
                     /locus_tag="O0394_000894"
     tRNA            25137..25225
                     /locus_tag="O0394_000894"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25172..25174,aa:Ser,seq:gga)
BASE COUNT         9134 a         3672 c         4509 g         8233 t
ORIGIN      
        1 acttacagct attgctacgg ttgaagctac taaaacaaag tatcgtaatt aaacttagtt
       61 caataataac taaataatga tagataacac actaatcgta tgaccgtatc actgtaataa
      121 agggtgaaca cgtgcgatta gtgtattttt atttcaaatt atgaaaacgt tttaatcata
      181 aattgctcaa ttaaattatt taatgatata ctacagttaa ataaacagtg ataaggggag
      241 aaattgaatg tcagtaagaa ttgaacatga tacttttgga gaaatagaag tacctgcaga
      301 taaatattgg ggtgctcaaa cagaaagaag taaacgtaat ttcccagttg gtaaagagcg
      361 tatgccaatc gaagtagttt atggttttgc acaactaaag cgtgcagcag caatagctaa
      421 ttttgattta ggaaaattaa gcgaggcaaa gaaagatgcc attgtatacg catgtgatca
      481 aattttatca ggtgaattag atgaacactt cccactagtt gtatggcaaa caggaagcgg
      541 tacacaaagt aatatgaatg tgaacgaagt agtaagttat gttgctaata tgtatttaaa
      601 agatcatcaa agtgatgaaa gtatccaccc aaatgatgat gtaaataaat ctcaaagttc
      661 gaatgataca ttcccaactg ctatgcacgt tgcattatat caagaggttg aaacaaaatt
      721 agaacctgca ttaaaacttt taagaaatac tttgaaagaa aaagaagata aatttgattc
      781 aattattaaa attggtcgta cacatttaca agatgcaacg ccgatcaaac taggacaaga
      841 gattagtggc tggcgttata tgcttgaccg ttgtgaaaca atgttatctg aatctaagaa
      901 gcacatttta aatcttgcca tcggtggtac ggctgttggt actggtatta atgcgcatcc
      961 tgaatttggt gataaagtgg cacattatat ttcagaaaat acgggttatc catttgtatc
     1021 ttctgaaaat aaattccacg cacttacagc gcatgatgaa gttgttcaat tgcatggaac
     1081 attgaaggca ttagcaggag acttaatgaa aattgctaat gatgtgagat ggttggcttc
     1141 agggccacga gctggtttgg cagaaatttc tatccctgaa aatgaaccag gttcatcaat
     1201 tatgcctggt aaagttaatc ctacacaatg tgaaatgtta acaatggttg cagtccaagt
     1261 aatgggtaat gatacagttg ttggcttcgc aagttcacaa ggtaactttg aattgaatgt
     1321 ttataaacca gttattatgc ataatacact acaatcaatt tatcttttag ctgatggtat
     1381 ggaaacattt aataacaatt gtgcagtggg cattgaacca atcgaagaga atattgataa
     1441 ttatttaaat caatcattaa tgttagttac tgcattaaat ccacatattg gttatgaaaa
     1501 agcagctcaa attgctaaga aagcccataa agaaggttta actttaaaag aatctgcaat
     1561 tcaaactgga tatgttacag aagaacaatt tgaagcatgg attaaaccag aagatatggt
     1621 agatcctcat taattaaata ttaatataaa ataaagtttg ggcattacag gtagatgtag
     1681 tgttcaagct ttattttttg tctatgtcaa attgaactta taagtctgat ttgatataga
     1741 gccatgaaaa ggaaaaactt ttcatggagt ccgttttatt atagaaccta ttttttatat
     1801 gatgaattgt tgtacatgta ttggccaaac aacctgtgat aaaaatctta tgatgtcatg
     1861 tattgtccat agatacacgt aacgcagttg aaaaagacta caagtcatta tccaaggatg
     1921 ttataaaatg aataattact aaaatatcaa aatataaaat cctaagcatc gtaaaaaaca
     1981 aaaaaaccga acatctgaaa aaattaataa ttttccatct atccgattta agttgatact
     2041 tgatttagtt acaatgcggt attgcatgtt taaatatcat gcaaaactaa tcaaattagt
     2101 catccaaaga aacggaaatg acttccttag tcaagggatg cacaaactct attttgtaac
     2161 tttcaagttc taattgtctt aacgttgaat caccatataa aggatcacca ataacagggt
     2221 gaccaatttc agctaaatgc acacgaattt gatgagtacg tccagtatcc aacttgattt
     2281 caagttggca cacagcttct tttatcattt ttgaagttaa aatgtgtgta attgcacgct
     2341 gtccagtagg agacacacgt cgtttattcg aatggaattt atctttacca attggcatat
     2401 caatcgtttg tggttttaaa ggtaaaagtg ccttaacatt tgctttgtat atccgcgtaa
     2461 tatcattgtc ttctaacatg cgatcaagaa tttttttcat taaaggattt ttagcaacaa
     2521 ttaataatcc tactgtttcc tggtccagtc gatgaattgg ttcgacatag tcactatcaa
     2581 tagtgtaaat cacatgattc attaaagtat tgctttcttt taaatcatta gggtgagtct
     2641 taacaccttt aggtttcatt acgattgcca tatcatcgtc ttcgtattta atttgtgcat
     2701 aacgataact tggaacataa ttactttttt catcgatggt tggaacaaat acatcgtcgc
     2761 cagtatgcac tttatccatt aatcgcgcag gtttatcatt aatagtaata tctttagaca
     2821 tatttaaatg atgtaaatct tttttaggta ccttaagttg ttggaaaata tctcgtaaac
     2881 ttaagtcatt aaagttttct ggtattttaa atttcataaa atcctcctat agtctttaat
     2941 atcatatcac aaatcagttc tccgaaatat aactattgag taatcaaggc cattttatca
     3001 tgtttacgtc taagatttaa aaatataata actcaatgta taatagttaa tacacgtaaa
     3061 aatgaattgg agcgatttga tggaacaaag gacgcgacta gctttattaa aagagattgc
     3121 cgaattttta aacgaagaaa ctgaaatgta tagcatgacc caaggtgcgt taaaatattt
     3181 aattgagggc agtaatttca cgacaggctg gatatttttc atcaatagcg taggtgaaca
     3241 tgaacttgta tcacatgtgg ctctaccaca atctttgaca gcagaccact gtcactatat
     3301 taaagatggg tcttgttggt gtgtgaaagc atttaatcaa cgtaggctaa tgaaagcgtc
     3361 gaatatcgtc aattgttctc gtattaactt agcctctaaa gcatttccta gccaaaatga
     3421 caatattaca catcatgcaa cggtaccact taaatcgggt caagaacaat tcggcatttt
     3481 aaatgtagcg tctccaaata ctgaaatata tagcgatgaa gatttagagt tgctagagtc
     3541 tgtcgcattt caattagggt cagccatcaa acgtatttat ttaacagacc gtgaaaaaga
     3601 agcggcaaaa attaatgaac gtaatcgttt agctagagat ttacatgatt cagtaaatca
     3661 aatgttattt tctgtaaaac taacagcaca tgctgcgtat ggcatgtcaa acgaatccat
     3721 cgctaaacaa gcattcaaga cgattgagga aacgagtcaa aatgccgtta atgaaatgcg
     3781 tgcattgatt tggcaactta agccagttgg acttgagcaa ggtttaattc atgctttgac
     3841 agcatatagt aaattaatgc atatacaatt aaatgtaaat gtagaaggtt taatcgattt
     3901 atctaatgaa atcgaagaaa acatatacag agcattacaa gagtgtatta ataatgttaa
     3961 gaaacatgct gatacgaata aaatggatct tacattaaaa caaatgaacg atatactata
     4021 tattgacgtg attgattatg gtcaaggatt tgagatcgat aatgttcaga tagcttcatc
     4081 acacggtatt aataacataa aacaaagagt taaattatta agaggtaaag tgacctttca
     4141 ttctcagcca acaaagggta cacaaataca attcacaatt cctataaaat agcttgattt
     4201 aaggagataa cccatgaaca aagtaatatt agtagatgac cattatattg tgcgacaagg
     4261 attgcgattt ttattatcca cgattgaaaa catagaagtt ttacaagact ttgcagatgg
     4321 agaaacattt ttagaatatt taaaagagca tgagcaccct gatattgtgc tattagattt
     4381 agtgatgcct ggcatgaatg gtattgaaat tacggaatat attaaggcac attatccgga
     4441 tattaaagtt ttggtattaa caagttatgt tgatgatgaa catgtaattt cagcaatcaa
     4501 taaaggtgct gatggttatg aaatgaaaga cgttgagcct cagcaattaa ttgaaactat
     4561 tagacgagtt atgaacggtg aaaaaatgat acatcctaag gcacaagatg tattcgaaac
     4621 agttagccaa aaaccacact acacgaataa gttgtcaaag agagaaattg aagtgttacg
     4681 tgaaatggtt aaaggtaaaa caaataaaga gattgcagaa actttatttg tatctgaaaa
     4741 aacaattaaa acacatgtca gtcatatatt tagtaaatta caagttagcg atcgtacaca
     4801 agcagcaatt tatgcaatgg aaaataagtt gatttagaat gagcttttaa atatttgtcg
     4861 aaaaatagac aatttttaat ctgattttga tgaatttttt atagcgatag ggtttattat
     4921 acagttttct ttgtaaattt gatatcatta taaaagtgta agttttatca aatctagtga
     4981 ttatgcataa aaatttactt catttacttt cgcttcatta taaagtaaac ttaactcttg
     5041 ttgacttgat caaaatttaa tacaaatgtg aaattgactc ttttcatttt attaaaaata
     5101 tattaagatg taatctgtgt ttcaagaaat gtgtattatt gcaatttctt gtgtccggat
     5161 aaaattcccc aaacagaaag gtagataaca gaatggatag acagagtttt acagatttaa
     5221 ttcaaacaaa atttaaaatg gttcgtatag aggctggtta tacgcaagat actatggcgc
     5281 aaacaattgg actttctaaa aagactttag tacaaatcga aaaagagaga gtattaccaa
     5341 actggacaac ttgtatttca atttgtgcat tattcagaga ctctgaagta ttaaacagta
     5401 catttggctg tgatccatta gaaatcgttc aaacaatttc aagaaatcac tgtgcatatc
     5461 caaaccatgc accaacaagt gatatttatt ggaataacat tgagactcgt aatgggtaca
     5521 ttttacaaag taacaaagta agcaatattt atcgtgtatt aaatccagat aaccaaccaa
     5581 ttttcggtac ttctaaaatg agagaagctg aaacatactt taacagaaat gctaaagaag
     5641 aattagtaca tatctaggcc tatatacttt taataaataa taaaagcgat gtaacacacc
     5701 tgtttcgaga ggtgaggcat cgctttttgt atgtaaacta ataataaatt gagcagaaag
     5761 tgaaatgatt aatgaattaa aaaataaaaa tagtggtata atgaataact atgttgtcac
     5821 aaaggagtca aaattatgaa tgcactattt attatcatat ttatgattgt agtaggggcg
     5881 ataattggtg gcattactaa tgtaattgca attagaatgc tgtttcaccc ctttaaacca
     5941 tactatatat ttaaatttag agtcccattt acaccaggtt taataccgaa aagacgcgaa
     6001 gaaattgcaa ctaaaattgg ccaagtgatt gaagagcatt tgcttacaga aactttaatt
     6061 aatgaaaaat tgaaaagcga gcaatcacag caagcaatag aatctatgat tcaacagcag
     6121 ttacaaaagt tgacgaaaga tcaattgtca ataaaacaaa ttacttctca aatcgatatt
     6181 gatttagaac aagtattaca aactaatgga aatcaatata ttgaatcaca attgaataat
     6241 tattatacaa agcatcaaaa ccaaacaata gcatctttat tgccaaatca acttgtaaca
     6301 tttttaaatc agcatgtaga taacgcaaca gacttattat gtgatcgtgc aaggaattat
     6361 ttatcatctg caaaaggcac acaagatatt aatgatatgt tggatacttt tttcaatgag
     6421 aaaggtaagt taattggtat gttgcaaatg tttatgacaa aagagagtat tgcagatcgc
     6481 attcaacaag aacttatacg tttaacatct catcctaaag caagaacaat tgtgacatcg
     6541 ttaattacca atgaatatca aacttttaaa gataaaccat tgaatgaact gttagatgca
     6601 tcgcagttta atgaaatcgc tgaaaatcta tcagtgtatg taacaacata tgcaagcaaa
     6661 caagcgaata agccagtggt tacgttaatg cctcaattcg ttgattattt agaaggtcaa
     6721 ctctctagca aattagcaaa tttaatcatt gaaaaattat cgattcactt atctacaatt
     6781 atgaaaaaag tggatttacg aggattaatt gaagaacaaa ttaatacgtt tgacttagat
     6841 tatattgaaa aattaattat agaaattgct aacaaagaac taaagttgat tatgtcatta
     6901 ggtttcattt taggtggtat tattggattt ttccaaggtt tggttgcaat ctttgtctaa
     6961 aaaagcgaga tgtggtaaag taatatcgaa cgttttaata aacatcacaa caaaggagtg
     7021 ctataaccat ggcagtaaat ttatatgatt atgcaaatca attagaacaa gctttaagag
     7081 aaagcgaaga atacaaagca atcaaagaag cattcgctaa tgtaaaagct aacgaagaat
     7141 ctaaaaagtt attcgacgag ttccgtgaaa ctcaaattaa cttccaacaa aaacaaatgc
     7201 aaggtgaaga aattgctgaa gaagatttac aaaaagcgca agaacaagcg caagcaattg
     7261 aaaaagatga aaacatctct gcattaatga atgctgaaca aaaaatgagt caagtattcc
     7321 aagaaatcaa ccaaattatc gttaaaccat tagacgaaat ttacgctgac taatattaaa
     7381 ataatatttt tcaatccttt aaatagagga atagactgcc gacagagtcc gagactttgt
     7441 gggtagtttt tttagttttg ataacggaag ttagaggctc tctgtcaaat tgggcagatg
     7501 cattcagtaa cggaagttag gctagtaaac gtgtcctaac ttcctttttg cattttgaag
     7561 tgtaaagttt tcaacataat actattagtt cggtcatgta tcggactgat ggaaaagcgt
     7621 ttcactttta atgactcatt aagaacggcc tgaaaatgtt tggcgtatta agtgcaatga
     7681 tagttttgac atttagtttc taattggtca ttactgccga gcaaatctag tagagtaatc
     7741 atgtaaatct ttaatgtgcc atttgattca ctagcggtgt taataactac ggaaattgca
     7801 tttccgactg aaatttttga aaaatatcaa cgtacgctac aaataaaatt ttaaactgtt
     7861 ataaatgtgt ctcaatttca tatgttcatc gacgatatga agcgtattat ggtaaaatga
     7921 agaaataata aacttgttaa taaataaaac atcacgattt gactaaagca ctttattatt
     7981 gtgtagataa tagtttttta acgaaataaa aatggcgact ggttttaata aatcagctaa
     8041 tgaatcacta cacctataag tatgaatata gtgattagaa tgctttgtat agttggattt
     8101 tgcaaaattg atgttagaac ctttaattaa ggagatatgg atgtatggtt aaatttattc
     8161 attgttcgga tttacattta gatagtcctt tcaaatctaa gagtcatata agccctaaaa
     8221 tttttgagga tgttcaaaaa agtgcttatg aaagttttaa aaatattgta gatattgcat
     8281 tacaacaaga tgttgatttt gtaattatcg cgggtgattt atttgatagt gaaaacagaa
     8341 cattacgtgc tgaaattttc ttaaaacagc aatttgaacg tttacaaaat gaacaaatct
     8401 ttgtttatgt ttgtcatggt aatcacgatc ctttatcatc aaagatttca tcaaactggc
     8461 cagataatgt ttctgtattt tcaaataaag ttgagacgta tgaagcaatt actaaatctg
     8521 gtgaaacaat ttatattcac ggatttagtt atgaaaatag agcaagttat gagaacaaga
     8581 ttgatgaata tccatcaagt caaggccaaa aaggcataca tattggtgtc ttgcatggta
     8641 cgtatagtaa atcttcagtt aacgaaagat ataccgagtt cattttagaa gatttaaaca
     8701 gtaaattgta tcattattgg gctttaggtc atatacatga acgtcaacaa ttaagtgata
     8761 tgcctgtaat taactattca ggtaatattc aaggtagaca ttttaatgag caaggtgaaa
     8821 aaggttgctt attaatcgag ggtgaccact taaaattaaa gactaaattt tatcctacac
     8881 agtatattag atttgaagaa gcaactattg aaacggataa gacatctaag caaggtttat
     8941 acgaggtcat tcaaaacttt aaagaacaag tgagagaaga aggaaaagcc ttttatcgtt
     9001 taacgcttgt tattaatagt gagacattaa tttcacctca agatttatta caagttgaag
     9061 aaatgattac agattatgaa gaaaacgaaa atcaatttgt atatattgat gagttaaaaa
     9121 tacaatatgc acaaaatgat gagtcacctt tagttaatga attttcagcg gaattattag
     9181 tcgatcaaac tgtttttgat aaagcgatgt cagatttata tttaaatcca agggcatcta
     9241 agttcctaga cgattatgga acattcgacc atacagcatt agttaatcgt gctgaagaaa
     9301 tattaaaagc tgaaatgaga ggtgaacaaa atgataatta aatcacttga aatttatggt
     9361 tacggtcaat ttgttcaacg taaaattgaa tttaataaaa acttcactga aatttttggt
     9421 gaaaatgaag cgggtaaatc gacgattcaa gcattcatcc attcgatatt atttggattt
     9481 ccaactaaaa agtctaaaga gccaagacta gaaccacgtc taggtaacca atacggtggt
     9541 aaattagtac ttattcttga tgatggctta gagattgaag ttgaacgaat taaaggcagt
     9601 gctcaaggtg atgtgaaagt atatttacct aatggtgctg tgcgtgatga tgcttggtta
     9661 caaaagaaac ttaattatat ttctaaaaag acatatcaag gtatcttttc atttgatgta
     9721 ctagggcttc aagacattca tagaaatcta aatgaaaaac aattgcaaga ttatttatta
     9781 caagcagggg ctttaggatc aactgaattc acgtcaatgc gcgaagtgat taatcgtaaa
     9841 aaagatgaat tatataaaaa atcaggtaaa aatccgatca ttaatcaaca aattgagcaa
     9901 ttaaaacaac tagaaagtca aattcgtgaa gaagaagcaa agctagaaac atatcatcgc
     9961 ttagtagatg atcgagataa atcatcacgt cgattagaga atttaaagca taatttaaat
    10021 caattatcaa aaatgcatga agaaaaacaa aaagaggttg ctttacatga tcattcacaa
    10081 gaatggaagt ctctagaaca acagttaaat attgagccaa tcacattccc agaaaaaggt
    10141 gtggatcgtt acgaaaaagc acgagcgcat aagcaatcgt tagaaagaga tattggttta
    10201 agaaatgagc gtttagctca acttaaagaa gaagcgactc aattagagcc agttaaacaa
    10261 tctgatattg acgccttcat tagtttgaat caacaagaaa atgaaattaa aaataaagaa
    10321 tttgaactta ctgcaatcga aaaggatatt gcgaataaac aacgtgataa agatgaattg
    10381 caatcaaata ttggttggtc tgaaacgcat catgacgtag atagttcaga ggcaatgaaa
    10441 agttatgtca gtgagcaaat caagaataaa caagaacaag ctgcatacat taaacaatta
    10501 gaacgtagtt tagaagaaaa taaaatcgaa gataatgcgg ttcatagcga actagattct
    10561 gttgaagaaa aaatagttcc tgaagagact tttgaaaaga aaaaagaata ctcacaacaa
    10621 gtcattgaat taaacgaaaa agagaacttg tatagcaaat tgaaagaacg tttcgaaatt
    10681 gagcaacaag aaaaacaaaa acgtcaaaaa ttgttacgaa caacgtttat tcttttaaca
    10741 ttagtaggta ttggtttaac tgccttctca tttatttcaa acaacatgtt gtttggtatt
    10801 attttcgcag tattaacttt agtatttgtt atcggtatca tcatgtctaa atcaaaagaa
    10861 gtagattata gcgaggccat cacagatgaa atcgaagaaa ttaaagcaca actagccatt
    10921 ttagatgaaa attatgactt agattttgat cttgatgagc aatatcgcat tcgtgatcac
    10981 tggcaacaag ctttgaaaaa taaagatatt ttagaagaga aacgtcagta tattgaaggt
    11041 cgtttaaatg atgcgaaagg gcgtcatgat gaattacaat cgacagttga gaacgttaag
    11101 gatgaattat acttatcatc taaaatttca aatgatttaa ttgttgatag tatttctaca
    11161 atggcgaata ttaaagcatt agatcaacat atcagtgatt taaatcaaca acgccagcaa
    11221 ttggttcaag aattagatac attctacaat catgctgaag ctgttacaaa atcacaattt
    11281 gtttatttca acaaattatc tttattccac gatgtgcaac aatggttgaa atcagctgaa
    11341 gacacgaatg aaaaatggcg tattaatgct gaaaatacca aacttgttac aaatgaatta
    11401 aatcatttaa atgctcaatt agaagaaaat aataaagaaa ttacagcact attcgatttt
    11461 atcaatgttg gtactgaaga agatttttac caacatcatg aagattacca aacttatact
    11521 agtaatttga gtcgttttaa tgatttaact aaatatcttg agaaccaaaa ctattcttat
    11581 gaattaagtt ctagtttaag tgaaaaaaca actgcacaac ttgaagaaga agatcattta
    11641 ttggctactc aagttgacga atataatgag caatatcttg aaatgcaagc acaagtcagt
    11701 gatttaagtg cacaaatcaa tcacatggaa actgatacaa cgcttgctaa tttaagacat
    11761 gaatatcaca gtcttaaaaa tcaacttaat gatatcgcaa aagattgggc aagtttaagt
    11821 tatttacaaa gtttagttga tgaacacatt aaacaaatta aagataaacg tttgcctcaa
    11881 gttattaatg aagcggtaga aatattgaag catttaacag atggcagata tacgatgatt
    11941 aactataatg aagattcaat tacggttaaa catgttaatg gtcaattata tgatcctgtt
    12001 gaactaagtc aatctacaaa agaattactt tatgtagctt tacgtatcag tttaattaag
    12061 gtactaagac catattatcc gttcccatta attgttgatg atgcatttgt tcattttgat
    12121 aaaaaacgta ctgaaaaaat gttgaattat ttacgatcat tatcagaaca ctatcaagta
    12181 ctatacttta catgtgtaaa agataatatt gttccatcaa aagaagtgat tacattaaac
    12241 aaaatagagg aaggcgggaa acgatgagaa atatagagaa tctaaatccc ggagattcag
    12301 ttgatcactt tttcttagtg cataaagcta cacagggtgt aacagcacaa ggtaaagatt
    12361 atatgacatt acatttgcaa gataaaagtg gtgaaattga agcgaaattt tggacggcta
    12421 caaaaaatga tatggcaaca atcaagcctg aagaaattgt acatgttaaa ggtgacatca
    12481 taaactatcg cggaaataaa cagatgaaag tcaaccaaat tagactagcg acaactgaag
    12541 atcaattaaa aacagaacaa tttgtagatg gtgcaccttt atcaccggca gaaatacaag
    12601 aagagatttc tcattatttg ctagatattg aaaatgctaa tttacaacgt atcacacgtc
    12661 atttattgaa aaaatatcaa gaacgatttt acacatatcc agctgctagt tctcatcatc
    12721 ataactttgc gagtggctta agctatcatg tattaacgat gttacgtatt gcaaaatcaa
    12781 tttgtgacat ttatccattg ttaaacaaaa gtttgttata tagtggtatt attttgcatg
    12841 atattggtaa agttagagaa ttgagtggtc ctgttgcgac gtcgtataca gtcgaaggta
    12901 acttattagg acacatctcg attgcgagtg atgaagtagt tgaagcagct cgtgaattga
    12961 acattgaagg agaagaaatc atgttgttaa gacatatgat tttatctcat catggtaagt
    13021 tagagtatgg ttctccaaaa ctgccatact taaaagaagc agaaatttta tgctatatcg
    13081 ataatatcga tgctagaatg aatatgtttg aaaaggcata taaaaaaact gacaagggtc
    13141 agtttacaga taaaatattt ggtcttgaaa atcgtagatt ctacaatcct gaatcactcg
    13201 attaagaatt aaaagatatc ggacagatga gtatactgaa acatgagctc atctgtccga
    13261 tttttaatgc gaaaaaaccg agcggccgtg gtatgatttt tgaaccacgg tcgctcggtt
    13321 ttgtgttatt ggctcatgcc ggattgtccg ccttgtgcgc caccttgttt aagtttttca
    13381 gggtttaaga ttttatcttc gacaactgat ttaatatcac gatctttaaa gtcaacatcg
    13441 tattctttta atagatcttt gtatgcatca gtcaataatt ttggattttt ttgtactttc
    13501 tgatcgacta atttttcttt caggctttgt ttttcactgt taaagtctgt tggtttatca
    13561 gctttaataa tatgatatcc aaagcttgat ttaacaacct ctgatacttc accatcttta
    13621 agcttaaata gtgctttttc aaaatcttta tcagtttgtc ctttaagaac ataacctaat
    13681 tcgccatctt ttttagctga accagtatcc attgattctt ttttagcgat ttcaccaaat
    13741 ttacttggat cttttgaaac ttctttttga atttcttcag ctttttgttt cgcttcttta
    13801 tcatctaagc cttctttgtc gcttttctta gatttaactt taattaaaat gtgtgaagct
    13861 ttcttgctgt cttctttaat ttcagaatca gagattttaa ttttatctga tagtaattct
    13921 ttatgataag cagcagtacg taaattttct ttatatttat cggctgttaa accttgctgt
    13981 tgaagggcct tttcaaattt atctttaccg ccgtattgct tttgcatttt ttcaatttgt
    14041 tcgtcaatct tcttatcatt aactttattt ttatatttat cagctaaaat tttatttaac
    14101 atttcagtaa atgatgcatt tgcaatttga tctttaccga tttttttcat tgtatctgca
    14161 actgttacgt ctccagcttt agaagaaatt aatgtatttt ctttagagtc tgtggcacta
    14221 gcgccacaag cgcctaataa taaagcacta gctgttaccg gaacgattaa tttgtttatc
    14281 atcttcatag ttgaaactcc tttgtaagtt aatcatcgtg aatataacat agttttatat
    14341 tatcaccaat caataaccca tgacatcaga ctagagcatt aggtttaggg gtagaaattg
    14401 atgaaaataa aaaaacaagg tctatataca tttgattgta agctattaca tagcctaaaa
    14461 acaaataata acaccttgct ataattattt tgttataaat gatatggatt tttaattttt
    14521 ttcaaaatat taatacctgt actatgttct tctatatatt gtccataatt agaaaatata
    14581 attattttta aatataataa atctgtaatg gaaataccga aattcaatgc caataagaac
    14641 ataaaataat gtccatattg cgggaatgaa attgttagat aaacgaacat tgcagataag
    14701 ataattaacg gcaatattaa gttaatgcaa aaataaaatt tgtgtacagg ttgattgacg
    14761 taggtattat agaatataag ccattttctt ttagttaact tatgaacttt aaacgatttt
    14821 ctatatggta agaaaaataa taaatgtata cttttatgta atggatacgt cataaataca
    14881 agtaataaaa agatgagaaa atgtctatct gataatggcg tattagataa aaaatacatt
    14941 acttcataac taactaaaaa cataatgatg gttgcaactg cactcataaa tgcaattcta
    15001 ggcaaaccaa atcgtgcatt gatatcaatt tgtcttttac ataaaaacat atcttacgcc
    15061 tcatctcttt tcaatataag ttagattata actaactcag gttcagtaat tcaatagtaa
    15121 catatcatgt tagaagaatt gttatataag aagaaaggag cgacgtattg tttgatttga
    15181 aacgcgaaag tggggattgg taaaattgtc tgattgacta gcgtagaagt agaatgcaga
    15241 acaaatttct gcgaacacat attcttgcta ataaagcatc acttatgaaa aaggcgcgtg
    15301 tgacatgagt atttgaaaac ttgaataata taaaaaatgg atatggcagg gcatatgcaa
    15361 aagcatcttt tgaccaacta agttatacaa atgctaaaaa tatagtgata tgtaattata
    15421 ataaacacca gattaataat ttgtgcaagt aaggtatatg taatgtgtat ataaggttcg
    15481 gctcaatgaa aatataagta tgttgtttgt cgataatgat ttaagtaaag cgtaatcaaa
    15541 gtgcaaggat aacacaatga atcctaagca atgttttcaa tcaattcata aatatgattt
    15601 aagcgaaaat cactacaaac aatgtcttcc tataatttgt aaacataagt tcatttgagt
    15661 tttaaaaaag agatgacaag aaatatctaa tttctaatgt catctcgttt atttggcatt
    15721 taattttaaa aaataaatat gctgttacta ataaaaatag ttgtgatcga gaacgtaacc
    15781 tgctacttag aaatttcgtt tccaatatcc tcgccacgat tttgtaaatt ttcgatgtgt
    15841 gactgtaaac gttcaatatt aggattaatg tcagatttga agtttccaag taatgactta
    15901 atttcaccac caattgaact accaaattcc gcactttcgt ttttaatttg aacaccatag
    15961 tttaaaatgt cattaaaact ttgtttaatc gtttcaaatt cacgttgtag ttctgatttt
    16021 gaaccagtag gggcagtacg atcgatcgtg ttgttcttga cacttttgtc gtcacgtcct
    16081 tgaagtgcaa ctacaaaacc agctgctacg ccaacaccga taccgaatag aatgcgtgat
    16141 gctttcattt atattctcct cacttttaac atctgttatc ttgataactc attttgttta
    16201 aaattgctaa ccttgatgtt atcacttcaa acgcaattgt tataatatta atcatattat
    16261 acccgttcga gcttagatta agcatacatt taaaattgtg cttgaatttg ttcagcaatt
    16321 tgttgttttg catcgttatc taaaatgtct tcatgtgttt cccacttata accaaatcca
    16381 tcaatatcat tttcgtatcg aggaattaag tggaaatgaa tatgaaatac agattgatct
    16441 gcaaactcac cattattttg aataatgttt aaaccatcag gattaaatgc acgcttaata
    16501 gcatttgcta ctttaggtaa tgctgcaccg atatgtttca ttgtttcttc atcagtttca
    16561 aagatattag cagaagcttt tttaggaatt aataacgtat gtcctttagt aacttgtgat
    16621 atatctaaaa aggcatagac ataatcgtct tcatatactt taaagctagg aatttctcca
    16681 gttaaaattt tgccgaaaat tgtttctgac atgatgacta cactcctttt caactagtat
    16741 taccattcat tataacattg tcgctaaaaa ttcgatatta ggacatgtgt atttaatatg
    16801 agcattttat aatttttggt acaatataag tcatggaggt gccttatgac agttaaagta
    16861 gaacagctta caggtggata tggaaaacgc cctgtaatta aagatataaa ttttgaatta
    16921 aacaaaggcg aaattgttgg acttattggc cttaatggcg ctggtaagag tacaacgatt
    16981 aaacatatgc taggattgct tacacctatg gaaggatctc tatcaatttc agatatcaac
    17041 attaatgatg acatagaggc ttatagaaga aagttatctt atattccgga atcaccggtt
    17101 atttatgaag aactcacatt agaggaacac attgagatga cagcaatggc atatgatatt
    17161 gatcgtgatg aaacgatgaa tcgagcaatg ccattattaa agacattccg tttagaaaat
    17221 gagttgaaag tttttccgag tcatttttct aaggggatga aacaaaaagt catgattatt
    17281 tgtgcgttta tcgttaatcc cgaattgtat attatagacg aaccattcct tggcctagat
    17341 ccattaggaa ttcaatccat gttagattta atggttgaaa agaaaaacga aggtagaacc
    17401 gttctaatga gtacacatat tttagcaaca gctgaacgtt actgtgatcg ttttatcata
    17461 ctagacgaag gtgaagtcgt tgcatttgga gatttagaag cattgagaca acaaactggc
    17521 ttacataatc aaacattaga cgatatatat attcatgtga cgcaaggtgg agatgtacat
    17581 gcgtaaccaa gctaccacat tatttaataa aagattacat gcccttcgta aagagaaaaa
    17641 ttattataat aagtttatat ttaatggtca tttcatggtc tttttactta ttttgctggg
    17701 tgcatttatt tttggatatg gtgaatggtt aaaacatatt ccaacgaata tcaatttttc
    17761 attaatagca gcagtgattg ttgcgttgac atctattttt ccaatgcgac cactgttaaa
    17821 agaagcggat aaaatctttt tattaccatt tgaaaaacac atgtctcaat ttatgcgtca
    17881 tgcgatttta tatagttact ttgcgcgcat attaatccaa ctcattattg ttattgtaat
    17941 gttcccgttg ttttacaata ttaatcaaca taatgtggca ttttatattt gttttggagt
    18001 cagtgcatta atttttccat atgttggttt acgcttaaga tggcaatggt atcaatcggg
    18061 acttaaaaca tggcaagtta atttaatatc attcatcacc tttgctctaa catattactt
    18121 actattagct ccaaaatggt acatcgcatt tgtgatggtt gctttgcctg tgttaataga
    18181 atttttagtt aaaaaatata aaccaggctt tttgtatcct tgggagaaaa tgattgcaat
    18241 cgaacatcga catcatatga attattataa atttgttaat atgttcaccg atgtaaaaca
    18301 tttgaaagaa tctgcagtca gaagaagtta tctagatata cttttaccag ttccaaaggg
    18361 tagtaaattt aactctaatg cgatgtattt attcttattt attagaagct ttataagagg
    18421 gagagacgca tttaatatta tctttaggtt agtaattatt gcagttttat taatggtttg
    18481 gttatcatat ccattagtga cagcaattat tgggtgttta tttgtatata ttatattgtt
    18541 acaaatggca caattttatt cacagcaagc atatggctta tggcctcaag tgtggcctgt
    18601 acctgaagag aaggtaatca aagggtatga acagttttta tatagattaa tgtttgttat
    18661 ttgtactgtg tttgcagtga catttattat aaaacatatg acgttattct atgttgtact
    18721 cattttctat attgtaggtt tattaacgat tagaagtatt attaaaaagt tgaaatatca
    18781 ggaaacatta ttacgagact aaaaaacttc cattggcatg tatgttgtaa aggtgcatgt
    18841 aatgttgaac gccaaatgat acggcgttca gattacatta gcatctatac gttaacagca
    18901 taaccaatgg aagttttctt cgaatctatt cttttattgg gtatagatat ctttcaaaat
    18961 aacttgaatg ttgtccgctt gaaccaaagt aatgacttaa tgcgtctttt gaaaaatggt
    19021 cattaaaggc atcagattgc ttaaagtctt cgtatgcatg tcgatcagca aatccgaaat
    19081 aaattttgta tgttgtacct ttagcaggtc ttaacaaacg atagctttta aagccaccaa
    19141 agtttctgaa attatcgtct acactaatca gtttcttttc aagttgatat gcatgatctt
    19201 ctgttgatgg aatgaagatt gcacaataga aatgatgttc actgaattca ccaatttctt
    19261 taataacttc atatattgaa ggtgatttta aaacagtctc accatcagtt tcttcaaaaa
    19321 taactgaagt atctgatgct gaaaattgga atagttgatg ggtcgggtta ttgattttta
    19381 tctgatgtaa aaatccataa gtgccataag atgtatatag tttcttcata tgaaaggtcc
    19441 cccttatgtt tttatttatt atacgatgat aaactagtca ttaccactat taataattga
    19501 ttaaaattac tataatgaat cttgcgagat atttagaagt aatgatacaa aataatggac
    19561 tttagaattt aaatttacaa taaatagttc tatgattaaa tgtcagtttt atgacattta
    19621 tttattgaaa atacgaacga atgagcgata tgataatata gataagaatg attttaattt
    19681 aggaggcctt tatggtgcat aataaaaaca atacaatttt aaaaatgatc aaaggtgaag
    19741 aaacatcaca tacacctgtt tggtttatgc gacaagctgg ccgttcgcaa ccagaatatc
    19801 gaaaattgaa agaaaaatat tcactattcg atattacaca tcagccggag ttgtgcgctt
    19861 atgtaacaca tttaccagtt gataattatc atacagatgc agcaatttta tacaaagata
    19921 ttatgacacc attaaagcca attggtgtcg atgtagaaat taaatcgggt attggtccag
    19981 tgattcataa tccaatcaaa acaattcaag atgttgagaa actttctcaa atagaccccg
    20041 aacgagatgt accatatgta ttagatacaa ttaaactttt aacagaagaa aagttaaatg
    20101 tgccgctaat aggatttact ggggcaccat ttacattagc gtcatatatg attgaaggcg
    20161 gaccatcgaa aaattacaat tttacaaaag cgatgatgta tagagatgaa gcaacatggt
    20221 ttgctttaat gaatcattta gttgatgtat ctgttaaata tgtaacagct caagtcgaag
    20281 caggtgccga attgattcaa attttcgatt catgggtagg tgcattaaat gtcgaggatt
    20341 acagacgtta cattaaacca catatgattc gattaatcag tgaggttaaa gaaaaacatg
    20401 atgtgccggt aattttattc ggtgtaggtg ccagtcattt aatcaatgaa tggaatgatt
    20461 taccgattga tgtattaggc ctagattgga gaacgtctat taatcaggct caacaattag
    20521 gcgttactaa aacattacaa gggaatttag atccatcaat tttattagca ccatggaatg
    20581 tcattgaaga gagattgaaa ccaatattag atcaaggtat ggagaacggt aaacacatct
    20641 ttaatttagg acacggtgta ttcccagaag tgcaaccaga gacgttacgt aaagtaagcg
    20701 aattcgtaca cacatataca caaagataat catagtttta cattgaagca catgaaagat
    20761 aattcattta aaggaaggta ttatcaatga ctaaaaaaat gggattatta gttatggctt
    20821 atggcacacc ttataaagaa agtgacatag agccatatta tacagatatt agacatggta
    20881 aacgtccatc tgaagaagaa cttcaagatt tgaaagatag atatgaattt ataggtggtt
    20941 tatcaccatt agcaggtaca acagatgacc aggctgatgc gctagtttca gcattaaata
    21001 aagcatatgc agatgttgaa tttaaactat acttaggatt aaaacacatt tcaccattta
    21061 tcgaagatgc ggttgaacaa atgcacaatg atggcattac tgaagcaatc acggtagtac
    21121 tagcaccaca ttattcttca ttttcagtag gatcatatga caaacgtgct gatgaagaag
    21181 ctgcaaaata tggtattcaa cttacacatg tgaaacatta ttatgaacaa cctaaattta
    21241 ttgaatattg gacgaataaa gtcaacgaaa cattagctca aataccggaa gaggaacata
    21301 aagacacggt attagttgtt tcggcacata gtttgccaaa aggtttaatc gaaaagaata
    21361 atgatccata tccacaagaa ctagaacata ctgcgctttt aattaaagaa caatctaata
    21421 ttgaacatat cgcgattggt tggcaatctg aaggtaatac aggtacacct tggttagggc
    21481 cagatgtaca agatttaaca cgtgatttat atgaaaaaca tcagtataaa aactttatat
    21541 atacgccagt aggttttgta tgtgagcatt tagaggtgct ttatgacaat gattatgaat
    21601 gtaaagtagt ttgcgatgat attggtgcga attattatcg tccaaaaatg ccgaatacac
    21661 atccattatt tatcggtgca attgttgatg aaatcaagtc tatattttaa tgacgaaaga
    21721 agcgtgaaac gttgtgacta aatcagtggc tattatagga gcggggataa caggtttatc
    21781 aagtgcatat tttttaaaac agcaagatcc taatattgat gtaaccatct ttgaagcatc
    21841 gaatcgtccg gggggaaaga ttcaatcgta tcgtaaagat ggttatatga ttgaactagg
    21901 gcctgaatct tatttaggta gaaaaacgat tatgacagaa ttagcgaaag atattggatt
    21961 agaacaagat attgttacaa atacgactgg acaatcatat atttttgcga aaaataaatt
    22021 atatccgatt ccaggtggtt caattatggg tattccaaca gatattaaac catttgttac
    22081 tacaaaatta atatcgccac ttggtaaatt aagagcaggg tttgatttat taaaaaaacc
    22141 tactcaaatg caggatggtg acatttctgt tggtgcattt ttcagagcaa gattaggtaa
    22201 tgaggtactt gagaatttaa tagagccttt aatgggtggt atttatggta ccgatattga
    22261 taaattaagt ttgatgagta cgtttcctaa ttttaaagaa aaagaagagg cattcggaag
    22321 tctgataaaa ggtatgaagg atgagaaaaa taagcgtctg aaacaaagac aattatatcc
    22381 tggcgcaccg aaaggacaat tcaaacaatt taagcatggt ttaagctcat ttattgaagc
    22441 attagaacaa gatgttaaaa ataaaggtgt gacaatacgc tacaatacgt cagtggatga
    22501 tataattaca tctcaaaaac aatataaaat tgtttacaat gatcaactag aagaagtgta
    22561 tgatggcgta ttagtaacga caccacatca agtgttttta aattggttcg gacaagatcc
    22621 agcatttgat tactttaaaa cgatggatag tacgactgtt gcaactgttg tattggcatt
    22681 tgatgaaaaa gacattgaaa atacccatga tggtactggc ttcgtaattg cgagaacgag
    22741 tgatacagac attaccgcat gtacttggac atcgaaaaaa tggccattta ctacaccgga
    22801 aggtaaggtt ttgattcgtg cgtatgtagg taaaccaggt gatactgtgg ttgatgatca
    22861 tacagataat gaattagtat cgattgtacg tagagattta agtcaaatga tgacatttaa
    22921 aggtgatcct gaatttacaa ttgtcaatcg tttgccgaaa agtatgccac agtaccatgt
    22981 cggtcatatt caacaaatta gacagattca agcacatatt aaacaaacat atccacgact
    23041 tagagtaact ggtgcatctt ttgaagcggt tggactacct gattgtatta cgcaaggtaa
    23101 agttgctgct gaagaagtaa tcgcagagtt gtaagttaat tgttggatgg tgttggtgta
    23161 ggtgcgacgc ctttagataa tgtttgatga aatatagtca tttttaagtt atataatggt
    23221 ggaagtaatg tgtaaatata gatactttca gcgggaatta tttttgtgaa attgaagcag
    23281 agacaaaggt ttagctgttt ttgaatgata ttgaaatgat gacaatacac tttaattaga
    23341 aatgtcactt tgcgatataa catttctaat taaggtgttt ttattttaca attataggta
    23401 atcagtcatt tttttaaaaa tagaaaagaa gtagtaagtt gaaaggagag aatgagatga
    23461 cagatgtaat tattgtacat tctatgcatg gaaatagtag aaatcattgg tatcagtggt
    23521 tggagcataa cttaacttta gaaggatatg acgtaacatt atttaatttt gaatcccctg
    23581 aagcgaagac agtggatcag tggattgaag ctatgacgaa acaaattaat gtacgcaaaa
    23641 aggatacata ttttgtaaca catggtttag gttcaatcac agctttaaaa tatattgaaa
    23701 tgattgatca gccgattgaa ggctttttca gtatagcggg ctttaaagaa gatgcagaaa
    23761 atatagattt agatattgat ttaagtaatg taaccattga ttacgataat attaaaaaga
    23821 aagttgacaa tttttacgga ttgagttcta aaaatgacaa atacgtttca tataaagaga
    23881 cccagcgttt aatgaatgca ttagagggga acttgcgaat cgtggaagat ggagggcact
    23941 tcttagaaga ggatggcttc gaaacattta cagcgttgca agatcggatg caagattaca
    24001 tgacacgata gttagtgtat gggttgtgtt atgttgtgtc agtaggtagc tgctgatttt
    24061 gaaaaattgt tgtaattgag gcgtcaagca atgtcttagg gctacaatgt tatattggta
    24121 gtcgctgact gaaagaatat gcggtgtaac aagcttttat aatttctttt tcagaaggta
    24181 taggcgttgt aaactttata actcctatac ctttttaaat gctttaacac tatttatgac
    24241 aactattaga cacttatatg ataaaaggga ataagaagcg tttttttaca caaaaaactt
    24301 tgaaaatgtc gttgacgtac actacaaaaa tgaatatact aatatttgtg ttcaacagag
    24361 aacatgttgc tacgatattt aagtaattat ttaagagata acatttaaat taaattcaaa
    24421 aagttcttga ctttaggttt ttgattatgg tatattaatt aggtaattca aaataatatt
    24481 agcgcgggat agagcagttc ggtagctcgt cgggctcata acccgaaggt cggtggttca
    24541 aatccgcctc ccgcaataca tagtttttaa tttaataggt ctcgtagtgt agcggttaac
    24601 acgcctgcct gtcacgcagg agatcgcggg ttcgattccc gtcgagaccg ccattattat
    24661 taccattacg gttcagtagc tcagttggta gagcaatgga ttgaagctcc atgtgtcggc
    24721 agttcgactc tgtcctgaac cattcttaat tcatggcggt tgtggtgaag tggttaacac
    24781 atcggattgt ggttccgaca ttcgagggtt cgatcccctt cagccgcccc ataatcgttt
    24841 acattagcgg gtgtagttta atggcaaaac ctcagccttc caagctgatg ttgtgggttc
    24901 gattcccatc acccgctcca ttattttcta ttattccaca gtagctcagt ggtagagcta
    24961 tcggctgtta accgatcggt cgtaggttcg agtcctacct gtggagccat ggctccttgg
    25021 tcaagcggtt aagacaccgc cctttcacgg cggtaacacg ggttcgagtc ccgtaggagt
    25081 catacaagca gaagtgaaat atcgcttctg tttttttatt acatatttat tgttgaggaa
    25141 ggttgtccga gctggccgaa ggagcacgcc tggaaagtgt gtaggcgtca caagcgtctc
    25201 aagggttcga atcccttacc ttccgtaaag gcgcttaaat tggttttacc cattttaagc
    25261 gctattttta ttttggactc aatcccttga tatatctgca tttgagctat tatcctcatt
    25321 tttacacttc ttatttattt atatccattt aaaatttttt agccacaatg tgactaattt
    25381 ttgatgaata atcctaattt tagtcacaag attttgaagt ttagtcacaa aacaaatcat
    25441 tcagattttt ttctataaat ttagtttcat tatcttcaaa ttctttaatc aaatgtgaat
    25501 atgttttaag cgttgtatgt acatctgcat gacctaatcg ttttgaaa
//
LOCUS       DAKTLH010000025        24306 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.025, whole genome shotgun sequence.
ACCESSION   DAKTLH010000025 DAKTLH010000000
VERSION     DAKTLH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24306
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.025"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..290
                     /locus_tag="O0394_000895"
     CDS             <1..290
                     /locus_tag="O0394_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0498987.1"
                     /translation="KVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEFRYGPVTIG
                     SNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIRR"
     gene            298..1737
                     /locus_tag="O0394_000896"
     CDS             298..1737
                     /locus_tag="O0394_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCY0498988.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMAKQKFKQREYKRAAEYFEKVLEL
                     SPDDMEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYHNELFVSENDVMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            1804..2739
                     /gene="trxB"
                     /locus_tag="O0394_000897"
     CDS             1804..2739
                     /gene="trxB"
                     /locus_tag="O0394_000897"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCY0498989.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
     gene            3619..4530
                     /gene="rapZ"
                     /locus_tag="O0394_000898"
     CDS             3619..4530
                     /gene="rapZ"
                     /locus_tag="O0394_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCY0498990.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            4527..5522
                     /locus_tag="O0394_000899"
     CDS             4527..5522
                     /locus_tag="O0394_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCY0498991.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            5632..6576
                     /gene="whiA"
                     /locus_tag="O0394_000900"
     CDS             5632..6576
                     /gene="whiA"
                     /locus_tag="O0394_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCY0498992.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            7150..7737
                     /gene="clpP"
                     /locus_tag="O0394_000901"
     CDS             7150..7737
                     /gene="clpP"
                     /locus_tag="O0394_000901"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCY0498993.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            complement(7980..8882)
                     /locus_tag="O0394_000902"
     CDS             complement(7980..8882)
                     /locus_tag="O0394_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCY0498994.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKALFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLIKE"
     gene            9584..10213
                     /locus_tag="O0394_000903"
     CDS             9584..10213
                     /locus_tag="O0394_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HCY0498995.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDKASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
     gene            10973..11986
                     /locus_tag="O0394_000904"
     CDS             10973..11986
                     /locus_tag="O0394_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCY0498996.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            12039..13049
                     /gene="gap"
                     /locus_tag="O0394_000905"
     CDS             12039..13049
                     /gene="gap"
                     /locus_tag="O0394_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCY0498997.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            13188..14378
                     /locus_tag="O0394_000906"
     CDS             13188..14378
                     /locus_tag="O0394_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCY0498998.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            14500..15261
                     /locus_tag="O0394_000907"
     CDS             14500..15261
                     /locus_tag="O0394_000907"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCY0498999.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            15264..16781
                     /locus_tag="O0394_000908"
     CDS             15264..16781
                     /locus_tag="O0394_000908"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCY0499000.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGNLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            16911..18215
                     /gene="eno"
                     /locus_tag="O0394_000909"
     CDS             16911..18215
                     /gene="eno"
                     /locus_tag="O0394_000909"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCY0499001.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
     gene            18555..19013
                     /locus_tag="O0394_000910"
     CDS             18555..19013
                     /locus_tag="O0394_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499002.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            19080..19313
                     /gene="secG"
                     /locus_tag="O0394_000911"
     CDS             19080..19313
                     /gene="secG"
                     /locus_tag="O0394_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCY0499003.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            19429..20169
                     /locus_tag="O0394_000912"
     CDS             19429..20169
                     /locus_tag="O0394_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCY0499004.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPDEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDNEMDHFKPTET
                     LKELSEALDTIKEQVDEVLDPILVIQAENDNMIDPQSANYIYDHVDSDDKNIKWYSES
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            20203..22575
                     /gene="rnr"
                     /locus_tag="O0394_000913"
     CDS             20203..22575
                     /gene="rnr"
                     /locus_tag="O0394_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCY0499005.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSYRGQSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEGSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPNVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     NEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGRKKKGKQRKGKNQRNNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            22597..23061
                     /gene="smpB"
                     /locus_tag="O0394_000914"
     CDS             22597..23061
                     /gene="smpB"
                     /locus_tag="O0394_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCY0499006.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            23152..23513
                     /gene="ssrA"
                     /locus_tag="O0394_000915"
     tmRNA           23152..23513
                     /gene="ssrA"
                     /locus_tag="O0394_000915"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         8631 a         3524 c         4695 g         7456 t
ORIGIN      
        1 ataaagtaat gttagatatt ttttacccag aactgattac gattggtagt aacagtgtta
       61 ttggttacaa tgtaacaatt ttgacgcatg aagcattagt tgatgaattt cgttatggac
      121 cagtgacgat aggatctaac actttgattg gtgcaaatgc taccatttta cccggtataa
      181 cgattggtga caatgtaaaa gttgcagctg gtacggttgt ttcaaaagat ataccggata
      241 atggatttgc atatggcaac cctatgtata taaaaatgat taggaggtga caattttatg
      301 gcgcaaaaga ataataatgt aattccaatg acttttgatg atgcatttta tcgtaaaatg
      361 gctaaacaga agtttaaaca aagagaatat aaacgagctg ctgaatactt tgaaaaagtg
      421 ttagaattgt cacctgatga tatggaaatt caaattgatt atgcacaatg tctagtgcaa
      481 cttggtattg ctaaaaaagc agaacattta ttttatgaca atattattta taataggcat
      541 ctagaagata gcttttatga attgagtcag ctcaacattg aagttaacga accaaacaag
      601 gcattcttgt ttggtattaa ttatgttatt gttagcgacg accaagatta tagagatgaa
      661 ttagatcaaa tgtttgatgt gaaatatcaa agtgaagaac aaattgaact tgaagctcaa
      721 ttgtttgtag ttcaaatact attccaatat cttttttctc aaggtcgatt aaaagatgca
      781 aagaattatg tcttacatca accacaagaa gttcaagatc atcgtgtagt acgtaattta
      841 ttggcaatgt gttatttata tctcggtgaa tatgatacgg ctaaagcatt gtacgaagca
      901 ctattacaag aggatagtac agatatatat gcattatgcc attatacttt gctactttat
      961 aacactaagg aaaatgaaca atatcaaaaa tatttaaaaa tattaaacaa agttgtacct
     1021 atgaatgacg atgaaagttt taaattaggt attgtattaa gttatttaaa gcagtatcgt
     1081 gcatcacaac aattgttgta ccctttatat aaaaaaggga aatttttatc aattcaaatg
     1141 tacaatgctt tagcatataa ttattattat ttaggtgaag aagacgaaag tcattactac
     1201 tgggataaat tgaagcaaat ttctaaagtg gaaattggac atgcgccttg ggtaattgaa
     1261 aatagcaaag aagtttttga ccaacatatt ttgccattac ttcaaagtga tgacagtcat
     1321 tatcgtttat atggtatttt tttattggat caattaaatg gtaaagaaat tgtgatgacg
     1381 gaaagtattt ggcaggtttt ggaaaatcta aataattatg agaaattgta tttaacgtat
     1441 ttagttcaag gtttaacgct caataaatta gacttcattc atcgcggctt attaacgctt
     1501 taccataatg aattatttgt aagtgaaaat gatgtaatgg ttgcatggat taatcaaggt
     1561 gaactcataa ttgctgaaaa agtagattta actgatgttg agccatatat cggtgcgttt
     1621 atttatttgt attttaaaaa tcaacctcga aacgttacaa agaagcaaat tacaacatgg
     1681 ttaggcataa cacaatataa actgaacaaa atgattgaat ttctcttgag catatagatt
     1741 tatgaaaagt tagatttatt atataatgcg cataatgatt aataatgagg aggcgttaat
     1801 aaaatgactg aaatagattt tgatatagca attatcggtg caggtccagc tggtatgact
     1861 gctgcagtat acgcatcacg tgctaattta aaaacagtta tgattgaaag aggtattcca
     1921 ggcggtcaaa tggctaatac agaagaagta gagaacttcc ctggtttcga aatgattaca
     1981 ggtccagatt tatctacaaa aatgtttgaa cacgctaaaa agtttggtgc agtttatcaa
     2041 tatggagata ttaaatctgt agaagataaa ggcgaatata aagtgattaa ctttggtaat
     2101 aaagaattaa cagcgaaagc ggttattatt gctacaggtg cagaatacaa gaaaattggt
     2161 gttccgggtg aacaagaact tggtggacgc ggtgtaagtt attgtgcagt atgtgatggt
     2221 gcattcttta aaaataaacg cctattcgtt atcggtggtg gtgattcagc agtagaagag
     2281 ggaacattct taactaaatt tgctgacaaa gtaacaatcg ttcaccgtcg tgatgagtta
     2341 cgtgcacagc gtattttaca agatagagca ttcaaaaatg ataaaatcga ctttatttgg
     2401 agtcatactt tgaaatcaat taatgaaaaa gacggcaaag tgggttctgt gacattaacg
     2461 tctacaaaag atggttcaga agaaacacac gaggctgatg gtgtattcat ctatattggt
     2521 atgaaaccat taacagcgcc atttaaagac ttaggtatta caaatgatgt tggttatatt
     2581 gtaacaaaag atgatatgac aacatcagta ccaggtattt ttgcagcagg agatgttcgc
     2641 gacaaaggtt tacgccaaat tgtcactgct actggcgatg gtagtattgc agcgcaaagt
     2701 gcagcggaat atattgaaca tttaaacgat caagcttaat tcgaagtcga attaagatgt
     2761 tgagctgtaa attatttgga tatttatttt aatagtgtca tcacagcgtt aaaataatgt
     2821 cttactttta aattaaagca aattatatag aaaactagaa cttagtacgt atcatttgtg
     2881 cgtttcaatg agttctagtt tttttatatg ttatattaaa cttataactt tatgggagtg
     2941 ggacagaaat gataaagagc cactaatgat ttattatgta gtggttctta aacattagcc
     3001 acagctaatg tgtacttaaa aataggaata catgagtaaa actcatgcat aagaaatact
     3061 aatttctata gaaaaagtat tactttatcg ttgtcccacc ccaacttgca cattattgta
     3121 agctgacttt ccgccagctt ctgtgttggg gccccgccaa cttgcacatt attgtaagct
     3181 gacttttcgt cagcttctgt gttggggccc cgccaacttg cattgtctgt agaaattggg
     3241 aatccaattt ctctatgttg gggcccacac cccaactcgc attgcctgta gaatttcttt
     3301 tcgaaattct ctgtgttggg gcccacaccc caactcgcat tgcctgtaga atttcttttc
     3361 gaaattctct gtgttggggc ccctgactag agttgaaaaa agcttgttgc aagcgcattt
     3421 tcattcagtc aactactagc aatataatat tatagaccct aggacattga tttatgtccc
     3481 aagctccttt taaatgatgt atatttttag aaatttaatc tagacatagt tggaaataaa
     3541 tataaaacat cgttgcttaa ttttgtcata gaacatttaa attaacatca tgaaattcgt
     3601 tttggcggtg aaaaaataat ggataataat gaaaaagaaa aaagtaaaag tgaactatta
     3661 gttgtaacag gtttatctgg cgcaggtaaa tctttggtta ttcaatgttt agaagacatg
     3721 ggatattttt gtgtagataa tctaccacca gtgttattgc ctaaatttgt agagttgatg
     3781 gaacaaggaa atccatcctt aagaaaagtg gcaattgcaa ttgatttaag aggtaaggaa
     3841 ctatttaatt cattagttgc agtagtggat aaagtcaaaa gtgaaagtga cgtcatcatt
     3901 gatgttatgt ttttagaagc aagtactgaa aaattaattt caagatataa ggaaacgcgt
     3961 cgtgcacatc ctttgatgga acaaggtaaa agatcgttaa tcaatgcaat taatgatgag
     4021 cgagagcatt tgtctcaaat tagaagtata gctaattttg ttatagatac tacaaagtta
     4081 tcacctaaag aattaaaaga acgcattcgt cgatactatg aagatgaaga gtttgaaact
     4141 tttacaatta atgtcacaag tttcggtttt aaacatggga ttcagatgga tgcagattta
     4201 gtatttgatg tacgattttt accaaatcca tattatgtag tagatttaag acctttaaca
     4261 ggattagata aagacgttta taattatgtt atgaaatgga aagagacgga gattttcttt
     4321 gaaaaattaa ctgatttgtt agattttatg atacccgggt ataaaaaaga agggaaatct
     4381 caattagtaa ttgccatcgg ttgtacgggt ggacaacatc gatctgtagc attagcagaa
     4441 cgactaggta attatctaaa tgaagtattt gaatataatg tttatgtgca tcatagggac
     4501 gcacatattg aaagtggcga gaaaaaatga gacaaataaa agttgtactt atcggtggtg
     4561 gcactggctt atcagttatg gctaggggat taagagaatt cccaattgat attacggcga
     4621 ttgtaacagt tgctgataat ggtgggagta cagggaaaat cagagatgaa atggatatac
     4681 cagcaccagg agacatcaga aatgtgattg cagctttaag tgattctgag tcagttttaa
     4741 gccaactttt tcagtatcgc tttgaagaaa atcaaattag cggtcactca ttaggtaatt
     4801 tattaatcgc aggtatgact aatattacga atgatttcgg acatgccatt aaagcattaa
     4861 gtaaaatttt aaatattaaa ggtagagtca ttccatctac aaatacaagt gtgcaattaa
     4921 atgctgttat ggaagatgga gaaattgttt ttggagaaac aaatattcct aaaaaacata
     4981 aaaaaattga tcgtgtgttt ttagaaccta acgatgtgca accaatggaa gaagcaatcg
     5041 atgctttaag ggaagcagat ttaatcgttc ttggaccagg gtcattatat acgagcgtta
     5101 tttctaactt atgtgtgaat ggtatttcag atgcgttaat tcattctgat gcgcctaagc
     5161 tatatgtttc taatgtgatg acgcaacctg gggaaacaga tggttatagc gtgaaagatc
     5221 atatcgatgc gattcataga caagctggac aaccgtttat tgattatgtc atttgtagta
     5281 cacaaacttt caatgctcaa gttttgaaaa aatatgaaga aaaacattct aaaccagttg
     5341 aagttaataa ggctgaactt gaaaaagaaa gcataaatgt aaaaacatct tcaaatttag
     5401 ttgaaatttc tgaaaatcat ttagtaagac ataatactaa agtgttatcg acaatgattt
     5461 atgacatagc tttagaatta attagtacta ttcctttcgt accaagtgat aaacgtaaat
     5521 aatatagaac gtaatcatat tatgatatga taatagagct gtgaaaaaaa tgaaaataga
     5581 cagtggttct aaggtgaatc atgttttaaa taagaaagga atgactgtac gatgagcttt
     5641 gcatcagaaa tgaaaaatga attaactaga atagacgtcg atgaaatgaa tgcaaaagca
     5701 gagctcagtg cactgattcg aatgaatggt gcacttagtc tttcaaatca acaatttgtt
     5761 ataaatgttc aaacggaaaa tgcaacaacg gcaagacgta tttattcgtt gattaaacgt
     5821 gtctttaatg tggaagttga aatattagtc cgtaaaaaaa tgaaacttaa aaaaaataat
     5881 atttatattt gtcgtacaaa gatgaaagcg aaagaaattc ttgatgaatt aggaatttta
     5941 aaagacggca tttttacgca tgaaattgat cattcaatga ttcaagatga cgaaatgaga
     6001 cgcagttact tgagaggagc ttttctggca ggtggctcag tgaataaccc tgaaacatct
     6061 tcgtaccatt tggaaatttt ttctcaaaat gagagtcatg cagaaggctt aacgaaacta
     6121 atgaatagtt atgagttgaa tgccaaacat ttagagcgaa aaaaaggaag tattacgtat
     6181 ttaaaagaag cggaaaagat ttcggatttt cttagtttga taggtggcta tcaagcgtta
     6241 ttaaaatttg aagacgtacg tattgtaaga gatatgcgta attctgttaa ccgactcgtt
     6301 aattgtgaaa cggccaatct aaataaaaca gttagtgctg cgatgaaaca agttgagagc
     6361 attaaattga ttgataaaga aattggtatt gaaaatttac cagacaggtt gagagagatt
     6421 gctagaattc gagtagaaca tcaagaaatt tcgttgaaag agcttggaga aatggtatca
     6481 actggtccaa tttcaaaatc aggtgtaaat caccgattaa gaaaactgaa tgatttagcc
     6541 gataagatta gaaatggtga acaaatagaa ttataagtaa gaaggtgttt ttggtagtga
     6601 attatcaaaa acactttttt tatgattaaa aagtgtttaa tgaataatag tcgactaact
     6661 attaaattga gctggaaagt ttaatgaagg aaattcaata accaagtgat gcctaatttt
     6721 ctcattaaat agcatcatat gtatcaattt ggtcataaga aaatatgaat gtgataatta
     6781 attgagtccc tgaaagtccc tgatcataga caaaaacaaa atcagcataa ataagaatcc
     6841 cgacattgcg ggattctctg tattgaaaag tattgtattt tattaaaaca gcctccttga
     6901 agggaattga acccctatct taagaaccgg aatcttacgt gttatccatt acaccacaag
     6961 gagcaaatat gtaatcatat ctctgtacta tgagaagtaa aataagtaca atctaaaata
     7021 gatattatca gtttacaaag gaaagagaaa agcgtcaaac aatgtaacta tttaaagtca
     7081 aagtgtttga ccaaatttga cttaatatgt aaaataatga gtaacagtta ttacaaggag
     7141 gaaatataga tgaatttaat tcctacagtt attgaaacaa caaaccgcgg tgaacgtgca
     7201 tatgatatat actcacgttt attaaaagac cgtattatta tgttaggttc acaaattgat
     7261 gacaacgtag caaattcaat cgtatcacag ttattattct tacaagcgca agactcagag
     7321 aaagatattt atttatacat taattcacca ggtggaagtg taacagctgg ttttgcgatt
     7381 tatgatacaa ttcaacacat taaacctgat gttcaaacaa tttgtatcgg tatggctgca
     7441 tcaatgggat cattcttatt agcagctggt gcaaaaggta aacgtttcgc gttaccaaat
     7501 gcagaagtaa tgattcacca accattaggt ggtgctcaag gacaagcaac tgaaatcgaa
     7561 attgctgcaa atcacatttt aaaaacacgt gaaaaattaa accgcatttt atcagagcgt
     7621 actggtcaaa gtattgaaaa aatacaaaaa gacacagatc gtgataactt cttaactgca
     7681 gaagaagcta aagaatatgg cttaattgat gaagtgatgg tacctgaaac aaaataattc
     7741 aaagtaaaga gtagactaag ctgtctgctc tttttgtatg agtaaaccaa ggtgtcaata
     7801 atttgtttac tatactttga gcggaaatat gattgaatga agctagttga accgtaacta
     7861 tatgaaatgt tcccttcaaa gtagacattg aaaggaacat ttcaatcctt tgtttgtaag
     7921 tcgctctaga cattacattt agtacatatg ttgtttctaa tgctcattaa tggtattgat
     7981 tattctttaa ttaaatcttc aagtgccatt tttaaattac tatatttaaa ttggaatccc
     8041 aatgcttgaa ttttattagg taatactttt tgagtatcca atactactgt tgacatttga
     8101 ccaagtatga gacgcattgc aagacttggt gcccaagttt catgaggctt atgcatagct
     8161 cttgctaaag tgtagccaaa taaattttga cgttcaggta taggtgcagt taaattaaac
     8221 ggaccactag ctgactcgtt atttattaaa aataaaatag cttgaattaa atcattgata
     8281 tgaatccatg aataccattg ttgaccagaa cctaatttac caccaatgta atattcgtat
     8341 ggtagtttca ttgtttgtaa cgcaccgcct tcattcgata aaattatacc gaaacgaccg
     8401 atgacaactc gtgtacctaa ttgttcaaat tgttgtgcga aacgttccca ttgatacaca
     8461 atatctgata agaaatcaaa aggtaaagtt ttataaactt ctgtgtaact cataaataaa
     8521 tcaggaggat agtaaccagt ggcactagca ttaaataaag ctttaggtgc tttattacgt
     8581 gatttaaaca attcatataa agcttgcgta gattgaattc tacttagcat tagcgtttgt
     8641 ttatattccg gtgtccatcg tttattcaat gtagcacctg ctaagttgat gaccacatcg
     8701 atattttgag gaactttgtg ttcccaccca gatttagccc agttgacata tgaaattttc
     8761 ttatcatttg aaatttggtc gtgtcgcgtt aatatcgtga tatgtgaatc tgatttttta
     8821 atttcattaa ctaattgaga tccaaccata ccagtcccac cagtaattaa gtattgtttc
     8881 attatcattc accccatgta attttgtatt tagttactat ttaataatca ttattgttgt
     8941 tcaaaggtta tacattattt agacaataat atgtcaataa ctttttttga attgtatttt
     9001 aataaaaatg gtataagtta taataaaggt gtaccttcga taacgaataa acatctctta
     9061 aaagtatgtg taaaacgctg catgatacaa acgaaggtaa aaatttgact ccctttagta
     9121 gtggacccgt acgttaatcg tgcgggtcgt ttttatttat ttcttattcc cattatacat
     9181 caatttaaag cattaatttt ttaaacaaat ttaagaatac atagtaatat aacaatctaa
     9241 acataaaaac ttttaacaca acacttaaac caatgcttta attttcaata cgtagctata
     9301 atttgttgta aaatcaaaaa ggttaaaatg ttaattttca aaaaaggctc aaaatatgtt
     9361 tgatttagtt attaaatgtt aagatatata agactactat ttctttgtaa aaatgaatcc
     9421 gatttacgag tgagtaatag tgaaggcagt tttaagttga agaaggcaaa aagagtaaat
     9481 gtttattaat atttgtagaa actaggtaag caaattagtt gtgaaaatgt taatggttgc
     9541 gtgataattt ctatatttaa attagtttga agtgagggag agtatgtcga atcaaaatta
     9601 cgactacaat aaaaatgaag atggaagtaa gaagaaaatg agtacaacag cgaaagtagt
     9661 tagcattgcg acggtattgc tattactcgg aggattagta tttgcaattt ttgcatatgt
     9721 agatcattcg aataaagcta aagaacgtat gttgaacgaa caaaagcagg aacaaaaaga
     9781 aaagcgtcaa aaagaaaatg cagaaaaaga gagaaagaaa aagcaacaag aggaaaaaga
     9841 gcagaatgag ctagattcac aagcaaacca atatcagcaa ttgccacagc agaatcaata
     9901 tcaatatgtg ccacctcagc aacaagcacc tacaaagcaa cgtcctgcta aagaagagaa
     9961 tgatgataaa gcatcaaagg atgagtcgaa agataaggat gacaaagcat ctcaagataa
    10021 atcagatgat aatcagaaga aaactgatga taataaacaa ccagctcagc ctaaaccaca
    10081 gccgcaacaa ccaacaccaa agccaaataa taatcaacaa aacaatcaat caaatcagca
    10141 agcaaaacca caagcaccac aacaaaatag ccaatcaaca acaaataaac aaaataatgc
    10201 taatgataag tagtatttag tcaaacaaaa atgaaccagt atgacagaca acacaattaa
    10261 ttaggttgtc tcgaatattg gttcttattt ttataattgt taattagggg agagatgata
    10321 cttaaatagt tagttattta ttttacggat agtgaaattt attttgagtg aggtgggaca
    10381 gaaatgatat tttcgcaaaa tttatttcgt cgtcccaccc caacttccat tgcctgtaga
    10441 aattggaaat ccaatttctc tatgttgggg ccccacccca acttgcattg cctgtagaaa
    10501 ttgggaggag ccaatttcac tatgttgggg ccccacccca actcgcattg cctgtagaat
    10561 ttcatttcga aattctctat gttggggccc ctgactttaa ttgaaaaaag cttgttacaa
    10621 gtgcattttc gttcggttaa ctactactaa tgtgactttt tggattctag agcattgatt
    10681 tatgtcctag tctcaaataa taagcaaatg attatcgagt cagtataagg gtatacattt
    10741 gactacagcg aataaaataa aacgttttgt tgaataacaa atcgaaaata tattgcaagc
    10801 gctttatcaa attatttaga aaatttaagt tttatgcttg caatttttga aatagaatag
    10861 tactattgca agtgtaaaga ggttaatttt tgtcccacgc gggacttaaa aaggcaacca
    10921 ctggttgtga catatcctta tttacattta taaatataag gaggaggtag tagtgaaaga
    10981 cttattgcaa gcacagcaaa agcttatacc ggatctcata gataaaatgt ataaacgttt
    11041 ttctattctt actactatct caaaaaatca gcctgtcgga cgtcgaagtt taagcgaaca
    11101 tatggatatg actgaacgtg tactgcgttc tgaaacagat atgcttaaga aacaagattt
    11161 gataaaagtt aagcctaccg gaatggaaat tacagctgaa ggtgagcaac tgatttcgca
    11221 attgaaaggt tactttgata tctatgcaga tgataatcgt ctgtcagaag gtattaagaa
    11281 taaatttcaa attaaggaag ttcatgttgt tcctggtgat gctgataata gtcaatctgt
    11341 taaaacagaa ttaggtagac aagcaggtca attacttgaa ggcatattac aagaagacgc
    11401 gatagttgct gtaactggcg gatccacgat ggcatgtgtt agtgaagcaa ttcatttatt
    11461 accatataat gtattcttcg taccagccag aggtggacta ggcgaaaatg ttgtctttca
    11521 ggcaaacaca attgcagcca gtatggcaca acaagctggc ggttattata cgacgatgta
    11581 tgtacctgat aatgtcagtg aaacaacata taatacattg ttgttagagc catcagtcat
    11641 aaacacttta gacaaaatta aacaagcaaa cgttatatta cacggcattg gtgatgcgct
    11701 gaagatggcg catcgacgtc aatcacctga aaaggtcatt gaacaacttc aacatcatca
    11761 agctgtcgga gaggcatttg gttattattt tgatacacaa ggtcaaattg tccataaggt
    11821 taaaacaatt ggacttcaat tagaagacct tgaatcaaaa gactttattt ttgcagttgc
    11881 aggaggcaaa tcgaaaggtg aagcaattaa agcatacttg acgattgcac ccaagaatac
    11941 agtgttaatc actgatgaag ccgcagcaaa gataatactt gaataagaga taaaaagttt
    12001 aatacttttt aaatatcatt ttaaaggagg ccattataat ggcagtaaaa gtagcaatta
    12061 atggttttgg tagaattggt cgtttagcat tcagaagaat tcaagaagta gaaggtcttg
    12121 aagttgtagc agtaaacgac ttaacagatg acgacatgtt agcgcattta ttaaaatatg
    12181 acactatgca aggtcgtttc acaggtgaag tagaggtagt tgatggtggt ttccgcgtaa
    12241 atggtaaaga agttaaatca ttcagtgaac cagatgcaag caaattacct tggaaagact
    12301 taaatatcga tgtagtatta gaatgtactg gtttctacac tgataaagat aaagcacaag
    12361 ctcatattga agcaggcgct aaaaaagtat taatctcagc accagctact ggtgacttaa
    12421 aaacaatcgt attcaacact aaccaccaag agttagacgg ttctgaaaca gttgtttcag
    12481 gtgcttcatg tactacaaac tcattagcac cagttgctaa agttttaaac gatgactttg
    12541 gtttagttga aggtttaatg actacaattc acgcttacac aggtgatcaa aatacacaag
    12601 acgcacctca cagaaaaggt gacaaacgtc gtgctcgtgc agcggcagaa aacatcatcc
    12661 ctaactcaac aggtgctgct aaagctatcg gtaaagttat tcctgaaatc gatggtaaat
    12721 tagatggtgg tgcacaacgt gttcctgtag ctacaggttc attaactgaa ttaacagtag
    12781 tattagaaaa acaagacgta acagttgaac aagttaacga agctatgaaa aatgcttcaa
    12841 acgaatcatt cggttacact gaagacgaaa tcgtttcttc agacgttgta ggtatgactt
    12901 acggttcatt attcgacgct acacaaactc gtgtaatgtc agttggcgac cgtcaattag
    12961 ttaaagttgc agcttggtat gataacgaaa tgtcatatac tgcacaatta gttcgtacat
    13021 tagcatactt agctgaactt tctaaataat tttagtatag tttttattca aatacgctag
    13081 tgctcagaac tatttagcat taattaaagc ttatgagtaa gcggggagca caaacgcttc
    13141 tccgcttatt tttatataaa atttcctaat tacaaggagg aaacaccatg gctaaaaaaa
    13201 ttgtttctga tttagatctt aaaggtaaaa cagtcctagt acgtgctgat tttaacgtac
    13261 ctttaaaaga cggtgaaatt actaatgaca accgtatcgt tcaagcttta cctacaattc
    13321 aatacatcat cgaacaaggt ggtaaaatcg tactattttc acatttaggt aaagtgaaag
    13381 aagaaagtga taaagcaaaa ttaactttac gtccagttgc tgaagactta tctaagaaat
    13441 tagataaaga agttgttttc gtaccagaaa cacgcggcga aaaacttgaa gctgctatta
    13501 aagaccttaa agaaggcgac gtattattag ttgaaaatac acgttatgaa gatttagacg
    13561 gtaaaaaaga atctaaaaat gatccagaat taggtaaata ctgggcatct ttaggtgatg
    13621 tgtttgtaaa tgatgctttt ggtactgcgc atcgtgagca tgcatctaat gttggtattt
    13681 ctacacattt agaaactgca gctggattct taatggataa agaaattaag tttattggcg
    13741 gcgtagttaa cgatccacat aaaccagttg ttgctatttt aggtggagca aaagtatctg
    13801 acaaaattaa tgtcatcaaa aacttagtta acatagctga taaaattatc atcggcggag
    13861 gtatggctta tactttctta aaagcgcaag gtaaagaaat tggtatttca ttattagaag
    13921 aagataaaat cgacttcgca aaagatttat tagaaaaaca tggtgataaa attgtattac
    13981 cagtagacac taaagttgct aaagaatttt ctaatgatgc caaaatcact gtagtaccat
    14041 ctgattcaat tccagcagac caagaaggta tggatattgg accaaacact gtaaaattat
    14101 ttgcagatga attagaaggt gcgcacactg ttgtatggaa tggacctatg ggtgtattcg
    14161 agttcagtaa ctttgcacaa ggtacaattg gtgtatgtaa agcaattgca aaccttaaag
    14221 atgcaattac gattatcggt ggcggtgatt cagctgcagc agcaatctct ttaggttttg
    14281 aaaatgactt cactcatatt tcaactggtg gcggcgcgtc attagagtac ctagaaggta
    14341 aagaattgcc tggtatcaaa gcaatcaata ataaataata aagtgatagt ttaaagtgat
    14401 gtggcatgtt tgtttaacat tgttacggga aaacagtcac aagatgacat cgtgtttcat
    14461 cacttttcaa aaatatttac aaaacaagga gtgtctttaa tgagaacacc aattatagct
    14521 ggtaactgga aaatgaacaa aacagtacaa gaagcaaaag acttcgtcaa tgcattacca
    14581 acattaccag attcaaaaga agtagaatca gtaatttgtg caccagcaat tcaattagat
    14641 gcattaacta ctgcagttaa agaaggaaaa gcacaaggtt tagaaatcgg tgctcaaaat
    14701 acgtatttcg aagataatgg tgcgttcaca ggtgaaacgt ctccagttgc attagcagat
    14761 ttaggcgtta aatacgttgt tatcggtcat tctgaacgtc gtgaattatt ccacgaaaca
    14821 gatgaagaaa ttaacaaaaa agcgcacgct attttcaaac atggaatgac tccaattata
    14881 tgtgttggtg aaacagacga agagcgtgaa agtggtaaag ctaacgatgt tgtaggtgag
    14941 caagttaaga aagctgttgc aggtttatct gaagatcaac ttaaatcagt tgtaattgct
    15001 tatgaaccaa tctgggcaat cggaactggt aaatcatcaa catctgaaga tgcaaatgaa
    15061 atgtgtgcat ttgtacgtca aactattgct gacttatcaa gcaaagaagt atcagaagca
    15121 actcgtattc aatatggtgg tagtgttaaa cctaacaaca ttaaagaata catggcacaa
    15181 actgatattg atggggcatt agtaggtggc gcatcactta aagttgaaga tttcgtacaa
    15241 ttgttagaag gtgcaaaata atcatggcta agaaaccaac tgcgttaatt attttagatg
    15301 gttttgcgaa ccgcgaaagc gaacatggta atgcggtaaa attagcaaac aagcctaatt
    15361 ttgatcgtta ttacaacaaa tatccaacga ctcaaatcga agcgagtggc ttagatgttg
    15421 gactacctga aggacaaatg ggtaactcag aagttggtca tatgaatatc ggtgcaggac
    15481 gtatcgttta tcaaagttta actcgaatca ataaatcaat tgaagacggt gatttctttg
    15541 aaaatgatgt tttaaataat gcaattgcac acgtgaattc acatgattca gcgttacaca
    15601 tctttggttt attgtctgac ggtggtgtac acagtcatta caaacattta tttgctttgt
    15661 tagaacttgc taaaaaacaa ggtgttgaaa aagtttacgt acacgcattt ttagatggcc
    15721 gtgacgtaga tcaaaaatcc gctttgaaat acatcgaaga gactgaagct aaattcaatg
    15781 aattaggcat tggtcaattt gcatctgtgt ctggtcgtta ttatgcaatg gatcgtgaca
    15841 aacgttggga acgtgaagaa aaagcttaca atgctattcg taattttgat gccccaactt
    15901 atgcaactgc caaagaaggt gtagaagcaa gctataatga gggcttaact gacgaattcg
    15961 tagtaccatt catcgttgag aatcaaaatg acggtgttaa tgatggagat gcagtgatct
    16021 tctataattt ccgacctgat agagcagcgc aattatcgga aatttttgcg aacagagcat
    16081 tcgaaggctt taaagttgaa caagttaaag acttattcta tgcaacattc actaagtata
    16141 atgacaatat cgatgcggct atcgtcttcg aaaaagttga tttaaataat acaattggtg
    16201 aaattgcaca aaataacaat ttaactcaat tacgtattgc agaaactgaa aaataccctc
    16261 acgttactta ctttatgagt ggtggacgta acgaggaatt taaaggtgaa cgccgtcgtt
    16321 taattgattc acctaaagtt gcaacgtatg acttgaaacc agaaatgagt gcttatgaag
    16381 ttaaagatgc attattagaa gagttaaata aaggtaactt ggacttaatt attttaaact
    16441 ttgctaaccc tgatatggtt ggacatagtg gtatgcttga gccgacaatc aaagcaatcg
    16501 aagcggttga tgaatgttta ggagaagtgg ttgataagat tttagacatg gacggttatg
    16561 caattattac tgctgaccat ggtaactctg atcaagtatt gacggatgat gatcaaccaa
    16621 tgactacgca tacaacgaac ccagtaccag tgattgtaac aaaagaaggc gttacactta
    16681 gagaaactgg tcgcttaggt gacttagcac ctacattatt agatttatta aatgtagaac
    16741 aacctgaaga tatgacaggt gaatctttaa ttaaacacta atattgtaaa agatgttaag
    16801 taaacgctta atgacactta ttttttgaaa ataatagtaa tatcattttg ttaaatgaaa
    16861 gaataaagct ataataatta tagaataact atttaaagga gattataaac atgccaatta
    16921 ttacagatgt ttacgctcgc gaagtcttag actctcgtgg taacccaact gttgaagtag
    16981 aagtattaac tgaaagtggc gcatttggtc gtgcattagt accatcaggt gcttcaactg
    17041 gtgaacacga agctgttgaa ttacgtgatg gagacaaatc acgttattta ggtaaaggtg
    17101 ttactaaagc agttgaaaac gttaatgaaa tcatcgcacc agaaattatt gaaggtgaat
    17161 tttcagtatt agatcaagta tctattgata aaatgatgat cgcattagac ggtactccaa
    17221 acaaaggtaa attaggtgca aatgctattt taggtgtatc tatcgcagta gcacgtgcag
    17281 cagctgactt attaggtcaa ccactttaca aatatttagg tggatttaat ggtaagcagt
    17341 taccagtacc aatgatgaac atcgttaatg gtggttctca ctcagatgct ccaattgcat
    17401 tccaagaatt catgatttta cctgtaggtg ctacaacgtt caaagaatca ttacgttggg
    17461 gtactgaaat tttccacaac ttaaaatcaa ttttaagcaa acgtggttta gaaactgcag
    17521 taggtgacga aggtggtttc gctcctaaat ttgaaggtac tgaagatgct gttgaaacaa
    17581 ttatccaagc aatcgaagca gctggttaca aaccaggtga agaagtattc ttaggatttg
    17641 actgtgcatc atcagaattc tatgaaaatg gtgtatatga ctacagtaag ttcgaaggcg
    17701 aacacggtgc aaaacgtaca gctgcagaac aagttgacta cttagaacaa ttagtagaca
    17761 aatatcctat cattacaatt gaagacggta tggacgaaaa cgactgggat ggttggaaac
    17821 aacttacaga acgtatcggt gaccgtgtac aattagtagg tgacgattta ttcgtaacaa
    17881 acactgaaat tttagcaaaa ggtattgaaa acggaattgg taactcaatc ttaattaaag
    17941 ttaaccaaat cggtacatta actgaaacat ttgatgcaat cgaaatggct caaaaagctg
    18001 gttacacagc agtagtttct caccgttcag gtgaaacaga agatacaaca attgctgata
    18061 ttgctgttgc tacaaacgct ggtcaaatta aaactggttc attatcacgt actgaccgta
    18121 ttgctaaata caatcaatta ttacgtatcg aagatgaatt atttgaaact gctaaatatg
    18181 acggtatcaa atcattctat aacttagata aataattttc tttataatca aatgctgaca
    18241 taattttagt tgaggattat tatgacggta taaattaaat aaagattttg agttcacgct
    18301 taaataagtt cacgcttaaa tttatagcct gccacagagt tgagactgtg gtaggttttt
    18361 tattttgaag tattaatcat aacagactaa taatcatgag gtaactaata acacatattt
    18421 aacttgtatt cttaaactgg tataataaat ttatgttgaa atgaatattg tatgacaggg
    18481 tattcacttt tattaaaagg taaaattaaa taaaggtttt atagaacgta tttaaatata
    18541 tgaggagtaa acaaatggct gatagaacga ataaagaaat taaaacagga cgctttattg
    18601 caactgcatc aatcgtattc tcaatattat tgattattca ttactttgtt tcgttggata
    18661 atgcgactgc caaagcatta cttaatttaa cgaatcaaaa cacttcagat aaagcgattg
    18721 attacatttt aaacagcttt agattcactg gtattatgta tattttggct tatctagcag
    18781 gcttcatcac tttttggaat cgacatactt atgtgtggtg gtttatgttt gcagtttatg
    18841 tatcaaatag tttgtttacg ttgattaatt tatcaatcac aattcaagca ataaaagctg
    18901 cacacggtgc gtacttaaca ttgccaattt taattgttat tataggttcg gttgcattag
    18961 cgatttatat gcttgttgtt tctatcaaac gtaaaagtac atttaatcgc tagaaaattg
    19021 attttaacaa taaaaatatg atatactact tgtcgtatat aaggaacgga ggacaattta
    19081 tgcatacatt tttaatcgta ttattaatca ttgattgtat tgcattaata actgttgtac
    19141 tactccaaga aggtaaaagc agtggacttt caggtgccat cagtggtggt gctgagcagt
    19201 tattcggtaa acaaaaacaa cgtggcgtcg atttattctt aaatagatta acaattattt
    19261 tatcaatatt attttttgta cttatgattt gcataagtta tcttggtatg taaggtccgg
    19321 cgatgtaaat gtcgggcttt tttatttata attaagaatg taatagttta acaataagct
    19381 atgtaaaata tatagcctag ttaagtatgc aaagggagcg ttagatttat gcagataaaa
    19441 ttaccaaaac ctttcttttt tgaggaaggt aaacgtgccg tgttattact acatggtttt
    19501 acaggcaatt cgtctgatgt tcgtcaatta ggtcgatttt tacaaaagaa aggttataca
    19561 tcatatgcac cgcaatatga aggccacgcg gcaccaccag atgaaatact gaaatctagt
    19621 cctttcgttt ggtttaaaga tgcgttagat ggttatgatt atcttgttga acaaggttat
    19681 gatgaaattg ttgttgctgg tctatcatta ggtggggatt ttgctttaaa attaagctta
    19741 aatagagatg taaagggtat tgtaacgatg tgtgctccta tgggtggcaa aactgaaggt
    19801 gccatttatg aaggcttttt agaatatgca cgcaatttta aaaagtatga aggtaaagat
    19861 caagagacta ttgataatga aatggatcat tttaaaccaa ctgaaacttt aaaagaacta
    19921 agtgaagcat tagatacgat taaagagcaa gttgatgaag tgttggatcc tattttagtg
    19981 attcaagcag aaaacgacaa tatgattgat ccacaatccg caaattatat atatgaccat
    20041 gtagattctg atgacaaaaa tatcaagtgg tacagtgaat ctggacatgt tattacgatt
    20101 gataaagaga aagaacaagt atttgaagat atttatcaat ttttagagtc attagactgg
    20161 tcagaataaa aagagatttt aacattagaa aggaggggca taatgaattt aaagcaatct
    20221 atagaagaga ttattaatca acctgaatat gaacctatgt cagtgtcaga ttttcaagat
    20281 gcattaggtt taagcagtgc cgactcgttt agagatttaa ttaaggtgct tgtggagtta
    20341 gaacaatcag gattaatcga acgtacaaaa acagacagat accaaaaaaa gcatagttat
    20401 agaggtcaat caaaattgat aaaaggaacg ttaagtcaaa ataaaaaagg ctttgcattc
    20461 ttaagacctg aagatgagga tatggaagat atatttattc ccccgacgaa aattaatcgt
    20521 gccttggatg gagatactgt tattgtagaa atccatcaat caaaaggtga acataaaggt
    20581 aaaatcgaag gggaagttaa gtcgattgag aagcattctg taactcaagt tgttggtacg
    20641 tatagtgaag ctagacattt tggctttgtt attccggatg ataaacgtat tatgcaagat
    20701 attttcattc ctaaaggtca aagtttaggc gcagtcgatg gtcataaggt acttgtacaa
    20761 attactaagt atgctgatgg ttcagataat ccagaaggac atatttctgc tattttagga
    20821 cataaaaatg atcctggcgt agatatttta tctattatct atcaacatgg catagaaatt
    20881 gaatttcctg atgaagtgtt acaagaagct gaagcagtac ctgatcatat tgaaaatact
    20941 gaaattaaag gccgtcatga tttacgtgat gaattgacaa tcacaattga tggtgctgat
    21001 gctaaagact tagatgacgc aattagtgtt aaaaagttag cgaacggtaa tacgcaatta
    21061 actgtaagta ttgctgatgt cagctattat gtaacagaag gttctgcatt ggataaagag
    21121 gcatatgata gagcgacaag tgtatatctt gttgaccgtg taattccaat gattccacat
    21181 cgattaagta atggtatttg ttcattgaat cctaatgttg atcgtttaac tctaagctgt
    21241 cgcatggaaa tcgatgctag tggtcgcgtt gttaaacatg aaatttttga tagtgttata
    21301 cattctgatt atcgaatgac gtatgatgcg gtaaatcaga ttattactga aaaggatcct
    21361 aacattcgcg aacaatataa tgaaattacg cctatgctag atttagcaca agatttatct
    21421 aatcgtttga ttcaaatgag aaaacgacgt ggtgaaatcg attttgatat tagtgaagca
    21481 aaagtattag ttaacgaaga cggtatacca acagatgttc aattaagaca acgtggcgag
    21541 ggtgaacgtc taattgaatc atttatgtta attgcaaatg aaacagttgc tgaacatttt
    21601 agtaagttag atgtaccttt tatttaccga gtgcatgagc aacctaaatc agatcgctta
    21661 agacaattct ttgattttat tacaaacttt ggcatcatga ttaagggtac tggcgaagat
    21721 attcatccaa caacacttca aaaggttcaa gaagaagtag aaggtcgacc tgaacaaatg
    21781 gtcatttcaa caatgatgtt gcgttcaatg caacaagcgc attatgatga tgtgaacttg
    21841 ggacattttg gcttatcagc tgaatattat acgcatttta catcaccaat tagacgttat
    21901 cctgatttaa cagttcatcg tttaatccgt aagtatttaa ttgagaaatc aatggataac
    21961 aaagaagtga agcgttggga agacaaattg cctgagttag ctgaacatac ttctaaacgt
    22021 gaacgtcgtg ctattgaggc agaacgtgat actgatgaat tgaaaaaagc agaatatatg
    22081 attcaacata ttggtgatga atttgaaggt attgtcagct cagtagctaa cttcggtatg
    22141 ttcattgaat tgccaaatac gatagaaggt atggttcata ttgcgaatat gactgatgat
    22201 tattaccgtt ttgaagagcg tcaaatggca ttaattggtg agcgtcaagc taaagtattt
    22261 agaattggtg acacagttaa ggttaaagtg acgcatgttg atgtagatga acgattaatt
    22321 gattttcaaa ttgtaggtat gcctttaccg aaaaatgatc gatcacagcg cccagcgcga
    22381 ggtaagacaa ttcaagccaa aacgcgtggt aaatcattag ataaatcaaa atctgatgat
    22441 aagggtcgta agaaaaaagg taagcaacgt aaaggtaaaa accaacgtaa taatgataaa
    22501 tcaggtaata gtaagcataa gccattttat aaagataaaa gtgtgaaaaa gaaagcacgt
    22561 cgtaagaaaa aataagcagc aatgaggtga gtatgaatgg ctaagaagaa atcaccaggt
    22621 acattagcgg aaaatcgtaa ggcaagacat gattataata ttgaagatac gattgaagcg
    22681 ggaattgtat tgcaaggcac agaaataaaa tcaattcgcc gaggtagtgc taaccttaaa
    22741 gatagttatg cgcaagttaa aaacggtgaa atgtatttga ataatatgca tatagcacca
    22801 tacgaagaag ggaatcgttt taatcacgat cctcttcgtt ctcgaaaatt attattgcac
    22861 aagcgtgaaa tcattaaatt gggtgatcaa acacgtgaga ttggttattc gattgtgccg
    22921 ttaaagcttt atttgaagca tggacattgt aaagtattac ttggtgttgc acgaggtaag
    22981 aaaaaatatg ataaacgtca agctttgaaa gaaaaagcag tcaaacgaga tgttgcgcgc
    23041 gatatgaaag cccgttatta agcgatttag ttgcttaatc gggctatatt tgatatagtt
    23101 atatgtgctt ttgtaaatta caaaagtatg atttgtttga tttattattt cggggacgtt
    23161 catggattcg acaggggtcc cccgagctca ttaagcgtgt cggagggttg tcttcgtcat
    23221 caacacacac agtttataat aactggcaaa tcaaacaata atttcgcagt agctgcctaa
    23281 tcgcactctg catcgcctaa cagcatttcc tatgtgctgt taacgcgatt caaccttaat
    23341 aggatatgct aaacactgcc gtttgaagtc tgtttagaag aaacttaatc aaactagcat
    23401 catgttggtt gtttatcact tttcatgatg cgaaacctat cgataaacta cacacgtaga
    23461 aagatgtgta tcaggacctt tggacgcggg ttcaaatccc gccgtctcca tatttgtagc
    23521 ctacagcctt tgtggttgtg ggctttttta ttttgtgttt ttcaggggat aatgcattgc
    23581 agaatttgtt gtgagtattg atatagcagt gtttgtatag gtgtttattt gatggaggaa
    23641 agagtaataa gtgattatga attagttttt gagatataag gggacagtga tgtgtgtcaa
    23701 ataagtgtca aaaaagttgg attctgagtt ttacattcaa cattgttcat gaagaaactt
    23761 ctttatacgc aaaaaattct ccatgttata tatgtcaata taaaaatgtg aatcgtctac
    23821 acttaattgg ataaatggct actgaaaaag aacttttcat ttttgttacg tcactaagtg
    23881 ggtgtagtta taaagagatg agccgagttt tgatattttc attagaatca atatgcctat
    23941 taacacaatc agcaatagtt gacgagacgg aaataaaaga agtcgtagtt aagaaatgca
    24001 tttcacaaca taccattgta gccattttta ttgttttgga tgataaactc tttttggaat
    24061 ttttagtttt tataatttgc aactacacta cttcttttac taatattaat gtctaagtaa
    24121 tcgataaaaa attttccatt gaataaatga gaagttaaaa actttactta acctttctca
    24181 ttgcattttc ctattcacga ttttaagaac ccaacatact acaaacgaat tttaaaaggc
    24241 gagagtaaag cttacttgtt tattatacat atttaaaatc caagagtcag aacagactac
    24301 tcctct
//
LOCUS       DAKTLH010000026        22773 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.026, whole genome shotgun sequence.
ACCESSION   DAKTLH010000026 DAKTLH010000000
VERSION     DAKTLH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22773
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.026"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            186..1376
                     /locus_tag="O0394_000916"
     CDS             186..1376
                     /locus_tag="O0394_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCY0499007.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            1485..2729
                     /locus_tag="O0394_000917"
     CDS             1485..2729
                     /locus_tag="O0394_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HCY0499008.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVDEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(3232..4161)
                     /locus_tag="O0394_000918"
     CDS             complement(3232..4161)
                     /locus_tag="O0394_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCY0499009.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(4403..5782)
                     /gene="argH"
                     /locus_tag="O0394_000919"
     CDS             complement(4403..5782)
                     /gene="argH"
                     /locus_tag="O0394_000919"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCY0499010.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPSYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            complement(5772..6977)
                     /locus_tag="O0394_000920"
     CDS             complement(5772..6977)
                     /locus_tag="O0394_000920"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCY0499011.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            7328..8659
                     /locus_tag="O0394_000921"
     CDS             7328..8659
                     /locus_tag="O0394_000921"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCY0499012.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            8984..9559
                     /locus_tag="O0394_000922"
     CDS             8984..9559
                     /locus_tag="O0394_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCY0499013.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLVVFWIVGKKLEQHFMGSKKE"
     gene            9564..10088
                     /gene="lepB"
                     /locus_tag="O0394_000923"
     CDS             9564..10088
                     /gene="lepB"
                     /locus_tag="O0394_000923"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCY0499014.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDQDNNKHDSRQFGLIDKKDIIGNVSL
                     RYYPFSKWTVQFKS"
     gene            10104..10679
                     /gene="lepB"
                     /locus_tag="O0394_000924"
     CDS             10104..10679
                     /gene="lepB"
                     /locus_tag="O0394_000924"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCY0499015.1"
                     /translation="MKKEILEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIVGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            10839..14315
                     /gene="addB"
                     /locus_tag="O0394_000925"
     CDS             10839..14315
                     /gene="addB"
                     /locus_tag="O0394_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HCY0499016.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            14316..17969
                     /gene="addA"
                     /locus_tag="O0394_000926"
     CDS             14316..17969
                     /gene="addA"
                     /locus_tag="O0394_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCY0499017.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPKNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDSVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYRAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQIEDSSRPNVNISIVYFEDVSTETILDNDEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYRYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            18135..19037
                     /locus_tag="O0394_000927"
     CDS             18135..19037
                     /locus_tag="O0394_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCY0499018.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
     gene            19366..19755
                     /locus_tag="O0394_000928"
     CDS             19366..19755
                     /locus_tag="O0394_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HCY0499019.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGRSPFFKPLHMILRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHTMFWI
                     TIALIIITMVLGVILPLGPISKLFGIG"
     gene            complement(19926..21242)
                     /locus_tag="O0394_000929"
     CDS             complement(19926..21242)
                     /locus_tag="O0394_000929"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HCY0499020.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRRYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVSVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEINAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(21294..22118)
                     /locus_tag="O0394_000930"
     CDS             complement(21294..22118)
                     /locus_tag="O0394_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0499021.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            22232..22540
                     /locus_tag="O0394_000931"
     CDS             22232..22540
                     /locus_tag="O0394_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCY0499022.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
BASE COUNT         7795 a         3411 c         4119 g         7448 t
ORIGIN      
        1 tgcttgtaat catttaagta attatgtcat tataaatgta agggttttca aaatagacaa
       61 atttaatgaa acttagtgat tatttcatct tttttggtgg aaaagtatct gctttgaaaa
      121 ctaatgtaat tatttggata atgtttcatg tataatagtt ataaatgatg aaaaggggag
      181 atatgatgac taaatctgaa aaaattattg agttaacaaa tcattacgga gcacataatt
      241 atttaccatt gccaattgtc atttcagaag ctgaaggggt atgggttaaa gatcctgaag
      301 gcaataaata tatggatatg ttatctgcat attccgctgt taaccaaggt catagacatc
      361 cgaaaattat tcaagcatta aaagatcaag ctgataaagt gactttagtt tcacgtgctt
      421 ttcatagtga taacttaggt gaatggtacg aaaaaatttg taaactggca ggtaaagata
      481 aagctttacc aatgaataca ggtgctgaag cagtagaaac agctttgaaa gcagcacgac
      541 gctgggcata cgatgttaaa ggaattgagc caaataaagc agaaatcatt gcatttaatg
      601 gtaacttcca tggtcgaaca atggcgccag tttcattatc ttcagaagca gaataccaac
      661 gtggttatgg tccgttatta gatggattta gaaaagttga ttttggagat gtagatgcat
      721 tgaaagctgc aattaatgaa aatactgcag cagttttagt agaaccaatt caaggtgaag
      781 cgggtataaa tataccgcca gaaggatatt tgaaagcaat tagagaatta tgtgatgaac
      841 ataatgtctt atttattgct gacgaaatcc aagcaggatt aggtcgttcg ggtaaattat
      901 ttgctacgga ttgggataat gtaaaacctg atgtctatat tttaggtaaa gcactaggtg
      961 gtggagtctt cccaatttct gttgtattag cagataaaga agtattagat gtctttacac
     1021 ctggctcaca tggttcaaca tttggtggta atccacttgc ttgtgctgca tcaattgctg
     1081 cattagatgt tatcgttgat gaggatttac caggccgctc tttagaatta ggagattatt
     1141 ttaaagaaca attaaagcaa attgatcatc catcaattaa agaagtccgt ggacgtggtt
     1201 tgtttatagg tgtggaactt aatgaaagtg ctagaccata ttgtgaagct ttgaaagaag
     1261 aaggcttatt atgtaaagaa acgcatgata ctgtcattcg ttttgcacca ccattaatta
     1321 ttactaaaga agaattggac cttgcacttg aaaaaataag acatgtattt caataatgtc
     1381 aaaaagttgt aacaatcagt ttgtaagggc tttaaaaagg aatacgttat gttacaatat
     1441 gtttgaaagc gaaatcatta ggtaaagaag aggcgaaaag gatcatgact gagaacaata
     1501 atttagtaac ttctactcaa ggaattatta aagaagcatt gcataaattg ggatttgacg
     1561 aaggaatgta cgatttaatt aaagaacctt taagaatgtt acaagtgcgt atccctgtac
     1621 gaatggatga tggcacagta aaaacattca caggttaccg tgcgcaacat aatgatgctg
     1681 ttggaccaac aaaagggggc gtgcgtttcc acccagatgt tgatgaagaa gaagtaaaag
     1741 cattatcaat gtggatgact ttgaaatgtg gcattgtaaa cttaccatac ggtggtggta
     1801 agggtggtat cgtttgtgat ccacgtcaaa tgagcattca tgaagttgaa cgtttatcac
     1861 gcggatatgt aagagcaatt tcacaattcg taggtccgaa caaagatatt ccagcaccag
     1921 atgtatttac aaactcacaa attatggctt ggatgatgga tgaatatagt gcattagata
     1981 aatttaattc accaggtttc atcacaggta aaccaattgt attgggtggt tctcatggac
     2041 gcgacagatc aactgcacta ggtgtagtta ttgcaattga acaagctgca aaacgtcgta
     2101 atatgcaaat tgaaggtgcc aaggttgtta ttcaaggttt cggtaatgcc ggaagtttct
     2161 tagctaaatt cttatatgat ttaggtgcaa aaattgtagg tatctctgat gcttacggtg
     2221 cattacacga tccaaatggc ttagatatag attatttatt agaccgtcgt gatagttttg
     2281 gtacggtaac aaatttattt gaagaaacaa tctcaaataa agaattgttt gaattagatt
     2341 gtgacatttt agtaccagcg gctatttcaa accaaattac agaagacaat gcacatgata
     2401 ttaaagctag tatcgttgtt gaagctgcta atggacctac aacaccagaa gcaacacgta
     2461 ttttaactga acgtggtata ttattagttc cagacgtatt agcaagtgct ggtggtgtaa
     2521 cggtttctta cttcgaatgg gtacaaaata atcaaggtta ttattggtct gaagaagaag
     2581 ttgatgaaaa gctacgtgaa aaattagaag cggcatttga tacgatttac gaattgtctc
     2641 aaaaccgaaa aatagatatg agacttgcag catatatcat aggtattaaa cgtacagcag
     2701 aagcagctag atatcgtggt tgggcataat taattatcat atgtgattta acgagcttgg
     2761 gacagaaaac aaagccctaa gctcgttaat tttattttag cagtagttga ctgtaaaaca
     2821 atgcccgtgt aacacgctct tttcaaaaat agtcggggcc ccaacacaga ggctggtgga
     2881 aagtcagctt acaatagtgt gcaagttggc ggggccccaa cacagaggct ggcggaaagt
     2941 cagcttacaa tagtgtgcaa gttggggtgg agccccgaca cagagaaatt agctcctcaa
     3001 tttctacaga caatgcaagt tggcggggcc ccaacacaga agctggcgaa aagtcagctt
     3061 acaatagtgt gcaagttggg gtgggacgac gaaataaatt ttgcgaaaat atcatttctg
     3121 tcccactccc aaaaagacag cagtaggata atttcaattt ggaaatatct tactgctgtt
     3181 ttttatagta atagctatat ttttgtattt atgttttcta gtttaacatt actacttaat
     3241 gacttcttta tatttatcag cgaaatttgt aaagacacca tcaacgccat atttatttaa
     3301 tcgtaacata tcagcttttt cattcactgt ataaggatgt actataaatc ctaagtcttt
     3361 taaatgatgg gtattttgtt cagttaaatc tgtataatca ggacctaatc caatcgcata
     3421 agagcgtatc tcttttaagc gttggtcgtt aaattgttgt agttcacctt tatcaactaa
     3481 ttttactaat ggcacatgct tattttgacg atgaattttc tttaaacttt cgtcagaaaa
     3541 tgattgaatc attacatgtc cattttttaa tttattgtta tttaaaaggt gatgcttttt
     3601 caatgaagct aataattgtt cttccattcc tgggtataca tcaggtgact ttgtttcaat
     3661 ataatagttt gcattcgggc cataacgttc taaaatttca tctaaagtgg gtactttagc
     3721 atttttataa cttgctcttg cgtattttgg atatttttta ttaaaccaac ttcctgcatc
     3781 taactgtttt aattcatcaa gggtataatc ctcaacttta ccgtgtccat ttgttgtacg
     3841 gttaacagtt tcatcatgca tagcaactaa atggccatct ttggtacgtt gtaaatcaat
     3901 ttcgatataa gatgctttta actcattatg actcttatca tatgcttgaa acgtatgctc
     3961 gggtgcatag ccacttgcgc cacgatgtgc gatagtagtg aatcgctcat tcgttaaatt
     4021 tgtatgccat tgaatagcct gaggtttatt tgcaatttga tttgtttgtt cagcgccagc
     4081 agtaggtact gataaaaatc ccatagtaaa aacagcagaa gcagccataa atttagtgaa
     4141 gcttttcgaa gagttagtca tagtctttat cctttcatct ctttatttgt agtctaacaa
     4201 ttttaaaata ctacaaaaac ctgcttgtga aaacgcttta cacatttctt ttacaattta
     4261 taacattttt gtaatataaa tttaaaagaa ttatcaaaca ttttaggaat cttaaaaatg
     4321 tgatgttcaa caagacgaag agagcattaa aatacccaca atgtaatttt aatagacatt
     4381 gtgggtaggt agattaacgt atttattgtg atagtaattg tttagcaaca tcaagttgtt
     4441 gtttgaccga tgattgacct gttgaaccgt aactttgacg tcgttttaaa caattttcag
     4501 gctgcaaata atcgtaaata tcggcatcaa tactagaatg atgttgttga tatgttgcta
     4561 aaggaacatc taataaataa tgaccttgtt gtatacattc taagacgatt tttcctacaa
     4621 tttcatgtgc agttctaaat ggaatatttt tagttactaa ataatctgct agttccgttg
     4681 catttgaaaa atcttcttta acagtttgat tgagtcgttc tttattaatt gtcatcgttt
     4741 gaatcatacc ttcgaaaata cgtaaagaac ctttaattgt atggacagca tcgaataaac
     4801 cttctttatc ttcctgcata tctttgttat atgctagagg taatcctttt aaagtcataa
     4861 gcatgctcat taaatgacca gtcgttcgac caactttacc tctaattaat tctgccatat
     4921 caggattttt cttttgtggc ataatagatg agccagttga aaatgcatct gataatgtaa
     4981 tgaatttagc ttcgtctgtg gaccagaaaa taatttcctc tgcaaagcgt gataagtgaa
     5041 ccatcgttaa agaaatatta tgcaatgttt caataatata gtctctgtca ctaacagcat
     5101 ctaggctatt ctcatagaga ctgccaaagt tcaacaatgc tgttgtctcg tgtctatcga
     5161 tagggtatgt ggtaccactt aaggctgctg cacctaaagg attaatatcg attcgtttta
     5221 aactatcttc aaatcgttgt tggtctcgtt gtaacatcca aaaataagtc ataatatgat
     5281 gtgcaaatga aattggctgt gcacgctgta aatgagtata actaggcata attgtatcaa
     5341 cattattgga agcgatgtct acaattacac tttgtaacga cttaattaat gcgatgatat
     5401 cttgcacttg tttcttagtg tacaagtgca tgtctgttgc aacttgatcg tttctactgc
     5461 gtccagtatg caacttacca ccagcatcac cgatacgttt aattaattca tgttcaatat
     5521 ttaaatgaat atcttctaat gatgcactaa attgaatttg atcttgatga taatcatgtt
     5581 gaatagattt tagtccttgt ataatttgtt cgctgtcttg ttgactaata atgccttgat
     5641 tcgcaagcat agttgcatgt gcaatgctgc cttcgatatc ttgatctatg agcgtttgat
     5701 caaaagtaat ggatgcgtta aagtcgtcaa cccactcttc aggttgtact tcaaatctac
     5761 cgccccaagc tttattgctc attgctatag cctccatgca acatcgcatt tacttgagta
     5821 ggtaaaccat agatatcgat aaagccaaca gcagcgtctt gattaaatgc atcttctttt
     5881 gtataagttg ctaatttttc atcatataat gtgtaaggtg attttctacc attcacgatg
     5941 gcattacctt tgaataattt aattctgaca tcaccactta cgtattgctg agtactatca
     6001 ataaataatt tcaagctatc agttaaaggt gagaaccaaa gtccattgta tagttgttca
     6061 gcaaattgct tctcaatgat tggtttaaag tgtgcgacat ctttcgttaa cgtaatcgtt
     6121 tctaatgctt tatgcgcttt taaaataact tctgcagcag gtgcctcata aatttctctt
     6181 gatttgatac ctacaagtct attttctaca tggtcaattc ttccgatacc atgcttacca
     6241 gctaatgcat tcaacgttaa aattaaatcg tctaattcat atgttttgcc atcaatttga
     6301 actgggatgc ctttatcaaa cgttaaaatg atttcatcag cagtatctgg tgtttcttct
     6361 aaagcatttg ttagatcgaa cgcatcctct ggtggcgcag cataaggatc ttctaaaata
     6421 ccacattcat tcgctctgcc ccatagattt tgatcgatag aataaggtga atcatggttg
     6481 attgatacag ggatattatg tttaattgca taatcgattt cttcttcacg actccatgcc
     6541 cactcacgta caggtgcgaa tgctttcaat gatgggttta atgctttaat ggcaacttcg
     6601 aaacgtactt ggtcattccc tttaccagta caaccatgtg caatacctac tgaatttgtt
     6661 ttctcagcaa tctctactaa ttttttagcg attaatggtc ttgataaagc tgaaactaat
     6721 ggatatgcat tttcatacat taaatttcct ttgattgcat aacttacata ctcatcacta
     6781 aattcttttg ttgcatcaat aatatgacat tcaactgctc ccatatctaa agctttttta
     6841 taaacgatgt ctaaatcttt accttcacca acatctaggc aacaagctac aacgtcgtat
     6901 cctttgtcga taagccattg aacggccaca cttgtatcta gtcctcctga atatgctaaa
     6961 acaattttct ctttcataaa attcacctca ttgtagttat ctgaatattt cgtatgaaca
     7021 catgatagca aacttttaat gaaaataaaa ccattatttt acatatttat tcataaaatg
     7081 cgatagagat agttagagtt gtaagtgtaa atgcttattt atcaatgttt aaaagtattt
     7141 taaaaaacat gcaaattgta taaaaaatgt ttgagtgttt gacgccagtt tgaaatgttt
     7201 gttgaaaaat aggtgtgaaa cgtcacataa atatcaattt tattgattaa gtacaaactt
     7261 gtgaattgaa tcgcaaacgt aaacttagta aaattaagat attaaatgaa acaggaggct
     7321 tttatagatg actcatattc aattagattt tagtaaaacg ttagaatttt tcggtgaaca
     7381 cgaattaaaa caacaacaag aaattgttaa atcaattcac aaaacaattc atgaaggtac
     7441 tggtgcaggt agtgacttct taggctgggt tgatttacca gttgattacg acaaagaaga
     7501 attttcaaga attgttgaag catcaaaacg cattaaagaa aattctgatg ttttagtagt
     7561 catcggtatt ggtggttctt acttaggtgc acgtgcagca atcgaaatgt taacgtcatc
     7621 atttagaaac agcaatgaat accctgaaat tgtatttgtt ggtaatcact tatcatcaac
     7681 atatacgaaa gagttagttg attatttagc agacaaagat ttctctgtaa acgttatttc
     7741 taaatctggt acaactacag aaccagcagt tgcatttaga ttgttcaaac aattagttga
     7801 agaaagatac ggtaaagaag aagcacaaaa acgtatattt gcaacaacgg ataaagaaaa
     7861 aggtgcttta aaacagttgg ctacaaacga aggttatgaa acgtttatcg tacctgatga
     7921 tgtaggtgga agatattctg ttttaacagc agtaggatta ttaccaattg caacagctgg
     7981 aattaacatc gaagctatga tgattggtgc tgcaaaagca cgtgaagaat tatcttcaga
     8041 taaattagaa gaaaacattg cataccaata tgcgacaatt cgaaacattt tatatgcaaa
     8101 aggttataca acagaaatgt tgattaacta tgaaccatct atgcaatact ttaatgaatg
     8161 gtggaaacaa ttatttggtg aatcagaagg taaagacttc aaaggtatct atccttcaag
     8221 tgccaactac acaactgatt tacattcttt aggtcaatat gtacaagaag gccgtcgctt
     8281 cttattcgaa acagttgtaa aagtaaatca tcctaaatat gatattacta ttgaaaaaga
     8341 tagtgatgat ctagacggat taaattattt agctggtaaa acaatcgacg aagttaacac
     8401 aaaagcattc gaaggtacat tattagcgca tactgatggt ggtgttccta acatggtagt
     8461 gaacattcca caattagatg aagaaacttt cggttacgtc gtatacttct tcgaacttgc
     8521 ttgtgcaatg agtggatacc aattaggcgt aaatccattt aaccaacctg gtgtagaagc
     8581 atataaacaa aacatgttcg cattattagg taaacctggt tttgaagact tgaaaaaaga
     8641 attagaagaa cgtttataaa atacattact tcaaagatta gtgaagtttg aaaagataga
     8701 actagacgtt aactatttaa agcatatttt cgaggttgtc attacaaatg taaagatgta
     8761 atgacaacct cgtttttatt tatatgcaag aactaggtta ctagctaatg tgacaagatg
     8821 ttaagagaaa attaaagata aaataatatc tgccttacaa taatattgtt atactactag
     8881 agactgattt attagcatga ttacatgtta atgtttcttt acttagtaat taactttata
     8941 atgtaagaat aattatcttc aaccaaagaa agggattgat gatttgtcgt ttcatcaagt
     9001 agaagaatgg tttgagatat ttcgacagtt tggttattta cctggattta tattgttata
     9061 tattagagct ataattccag tatttccttt agcactctat attttaatta acattcaagc
     9121 ttatggacct attttaggta tattgattag ttggcttgga ttaatttctg gaacatttac
     9181 agtctatttg atctgtaaac gattggtgaa cactgagagg atgcagcgaa ttaaacaacg
     9241 tactgctgtt caacgcttga ttagttttat tgatcgccaa ggattaatcc cattgtttat
     9301 tttactttgt tttcctttta cgccaaatac attaataaat tttgtagcga gtctatctca
     9361 tattagacct aaatattatt tcattgtttt ggcatcatca aagttagttt caacaattat
     9421 tttaggttat ttaggtaagg aaattactac aattttaacg catcctttaa gagggatatt
     9481 aatgttagtt gtgttggttg tattttggat tgttggaaaa aagttagaac agcattttat
     9541 gggatcgaaa aaggagtgac atcgtgaaaa aagttgtaaa atatttgatt tcattgatac
     9601 ttgctattat cattgtactg ttcgtacaaa cttttgtaat agttggtcat gtcattccga
     9661 ataatgatat gtcaccaacc cttaacaaag gggatcgtgt tattgtaaat aaaattaaag
     9721 ttacatttaa tcaattgaat aatggtgata tcattacata taggcgtggt aacgagatat
     9781 atactagtcg aattattgcc aaacctggtc aatcaatggc gtttcgtcag ggacaattat
     9841 accgtgatga ccgaccggtt gacgcatctt atgccaagaa cagaaaaatt aaagatttta
     9901 gtttgcgcaa ttttaaagaa ttagatggtg atattattcc gccaaacaat tttgttgtgc
     9961 taaatgatca agataataac aagcacgatt caagacaatt tggtttaatc gataaaaagg
    10021 atattattgg taatgttagt ttacgatact atcctttttc aaaatggact gttcagttca
    10081 aatcttaaaa agaggtgtca aaattgaaaa aagaaatatt ggaatggatt atttcaattg
    10141 cagtcgcttt tgtcatttta tttatagtag gtaaatttat tgttacgcca tatacaatta
    10201 aaggtgaatc aatggatcca actttgaaag atggcgagcg agtagctgta aacattgttg
    10261 gatataaaac aggtggtttg gaaaaaggta atgtagttgt cttccatgca aacaaaaatg
    10321 atgactatgt taaacgtgtc atcggtgttc ctggtgataa agtagaatac aaaaatgata
    10381 cattatatgt caatggtaaa aaacaagatg aaccatattt aaactacaat ttaaaacata
    10441 aacaaggtga ttacattact gggactttcc aagttaaaga tttaccgaat gcgaatccta
    10501 aatcaaatgt cattccaaaa ggtaaatatt tagtgcttgg agataatcgt gaagtaagta
    10561 aagatagccg tgcgtttggc ctcattgatg aagaccaaat tgttggtaaa gtttcattta
    10621 ggttctggcc atttagtgaa tttaaacata atttcaatcc tgaaaatact aaaaattaat
    10681 atgaaacaaa tacaacatcg tttgtcggtt ttaatactga taaacgatgt tttattttgt
    10741 tagtaccaca ataaaagcta agttcgaaat gaacttataa taaatcaatc acaatcactt
    10801 tgtgttaaaa tatgtgtcaa aggaagtgag ggtttgtcat gacattacat gcttatttag
    10861 gtagagcggg aacaggtaag tctacgaaaa tgttgaccga aataaaacaa aaaatgaaag
    10921 cagatccgct tggagatcca atcattttaa ttgcgccaac tcaaagtaca tttcaattag
    10981 aacaagcctt tgtcaatgat ccggaattaa atggtagttt aagaacagaa gtgttgcatt
    11041 ttgaacgatt aagtcatcgt attttccaag aagttggtag ttatagcgaa caaaagttat
    11101 ctaaagctgc aacggaaatg atgatttata acattgttca agaacaacaa aagtatttaa
    11161 aactttatca atcacaagca aaatattatg ggtttagtga aaaattaaca gaacaaattc
    11221 aagattttaa aaaatatgca gtaacgcctg aacatttaga acactttatt gctgataaaa
    11281 atatgcaaac tcgaactaaa aataagttag aggatattgc tttaatatac cgtgagttcg
    11341 aacaacgcat tcaaaacgag tttattacgg gtgaggattc attacaatat tttattgatt
    11401 gtatgccgaa atcagagtgg ctaaaacgtg ctgatatata tattgatggt tttcacaact
    11461 tttcaacgat tgagtattta ataatcaaag gattaattaa atatgcgaag agtgtcacaa
    11521 ttatattgac gacagatggt aaccacgatc aatttagttt atttagaaaa ccatcggaag
    11581 tgttacgaca tattgaagaa atagcaaatg aactcaatat ttctattgaa cgtcaatatt
    11641 tcaaccaatt atatcgcttc aataatcaag atttaaagca tcttgaacaa gaatttgatg
    11701 cacttcaaat caatcgagtg gcatgtcaag gtcatatcaa tattttagaa tctgcgacta
    11761 tgagagagga aataaatgaa attgcgcgac gtatcatcgt tgatattcgt gataagcaat
    11821 tacgatatca agatattgct attttatatc gtgatgaatc ttatgcttat ttatttgatt
    11881 ccatattacc gctttataat attccttata atattgatac aaagcgttcg atgacacatc
    11941 atccggtcat ggaaatgatt cgttcattga ttgaagttat tcaatctaat tggcaagtga
    12001 atccaatgct acgcttattg aagactgatg tgttaacggc atcatatcta aaaagtgcat
    12061 acttagttga tttacttgaa aattttgtac ttgaacgtgg tatatacggt aaacgttggt
    12121 tagatgatga gctatttaat gtcgaacatt ttagcaaaat ggggcgtaaa gcgcataaac
    12181 tgaccgaaga tgaacgtaac acatttgaac aagtcgttaa gttaaagaaa gatgtcattg
    12241 ataaaatttt acattttgaa aagcaaatgt cacaagcgga aactgtaaaa gattttgcaa
    12301 ctgcttttta tgaaagtatg gaatatttcg aactgccaaa tcaattgatg acagagcgag
    12361 atgaacttga tttaaatggt aatcatgaaa aggcggagga aattgatcaa atatggaatg
    12421 gcttaattca aatccttgat gacttagttc tagtatttgg agatgaacca atgtcgatgg
    12481 aacgtttctt agaagtattt gatattggtt tagaacaatt agaatttgtt atgattccgc
    12541 aaacattgga ccaagtaagt attggtacga tggatttggc taaagtcgat aataagcaac
    12601 atgtttactt agtaggtatg aatgatggaa cgatgccaca accagtaact gcgtcaagct
    12661 tgattacaga tgaagaaaag aaatactttg aacagcaggc taatgtcgag ttaagtccaa
    12721 catcagatat tttacagatg gatgaagcat ttgtttgtta tgttgctatg actagagcta
    12781 agggagatgt tacattttct tacagtctaa tgggatcaag tggtgatgat aaggagatca
    12841 gcccattttt aaatcaaatt caatcattgt tcaaccaatt ggaaattact aacattcctc
    12901 aataccatga agttaaccca ttgtcactaa tgcaacatgc taagcaaacc aaaattacat
    12961 tatttgaagc attgcgtgct tggttatatg atgaaattgt ggctgatagt tggttagatg
    13021 cttatcaagt aattagagat agcgatcatt taaatcaagg tttagattat ttaatgtcag
    13081 cattaacgtt tgacaatgaa actgtaaaat taggtgaaac gttgtctaaa gatttatatg
    13141 gtaaggaaat caatgccagt gtatcccgtt ttgaaggtta tcaacaatgc ccatttaaac
    13201 actatgcgtc acatggtctg aaactaaatg agcgaacgaa gtatgaactt caaaactttg
    13261 atttaggtga tattttccat tctgttttaa aatatatatc tgaacgtatt aatggcgatt
    13321 ttaaacaatt agacctgaaa aaaataagac aattaacgaa tgaagcattg gaagaaattt
    13381 tacctaaagt tcagtttaat ttattaaatt cttcagctta ctatcgttat ttatcaagac
    13441 gcattggcgc tattgtagaa acaacactaa gcgcattaaa atatcaaggc acgtattcaa
    13501 agtttatgcc aaaacatttt gagacaagtt ttagaaggaa accaagaaca aatgacgaat
    13561 taattgcaca aacattaacg acaactcaag gtattccaat taatattaga gggcaaattg
    13621 accgtatcga tacgtataca aagaatgata caagttttgt taatatcatt gactataaat
    13681 cctctgaagg tagtgcgaca cttgatttaa cgaaagtata ttatggtatg caaatgcaaa
    13741 tgatgacata catggatatc gttttacaaa ataaacaacg ccttggatta acagatattg
    13801 tgaaaccagg tggattatta tacttccatg tacatgaacc tagaattaaa tttaaatcat
    13861 ggtctgatat tgatgaagat aaactagaac aagatttaat taaaaagttt aagttgagtg
    13921 gtttagttaa tgcagaccaa actgttattg atgcattgga tattcgttta gaacctaaat
    13981 tcacttcaga tattgtacca gttggtttga ataaagatgg ctctttgagt aaacgaggca
    14041 gccaagtggc agatgaagca acgatttata aattcatcca acataacaaa gagaatttta
    14101 tagaaacagc ttcaaatatt atggatggac atactgaagt tgcaccatta aagtacaaac
    14161 aaaaattgcc atgtgctttt tgtagttatc aatcggtatg tcatgtagat ggcatgattg
    14221 atagtaagcg atatcgaact gtagatgaaa caataaatcc aattgaagca attcaaaata
    14281 ttaacattaa tgatgaattt gggggtgagc aatagatgac aattccagag aaaccacaag
    14341 gcgtgatttg gactgacgcg caatggcaaa gtatttacgc aactggacaa gatgtacttg
    14401 ttgcagccgc ggcaggttca ggtaaaacag ctgtactagt tgagcgtatt atccaaaaga
    14461 ttttacgtga tggcattgat gtcgatcgac ttttagtcgt aacgtttaca aacttaagcg
    14521 cacgtgaaat gaagcatcgt gtagaccaac gtattcaaga ggcatcgatt gctgatcctg
    14581 caaatgcaca cttgaaaaac caacgcatca aaattcatca agcacaaata tctacactcc
    14641 atagtttttg cttgaaatta attcaacagc attatgatgt attaaatatt gacccgaact
    14701 ttagaacaag cagtgaagct gaaaatattt tattattaga acaaacgata gatgaggtca
    14761 tagaacaaca ttacgatatc cttgatcctg cttttattga attaacagag caattgtctt
    14821 cagatagaag tgatgatcag tttcgaatga ttattaaaca attgtatttc tttagcgttg
    14881 caaatccaaa tcctaaaaat tggttggatc aattggtgac accatacgaa gaagaagcac
    14941 aacaagcgca acttattcaa ctactaacag acttatctaa agtatttatc acagctgctt
    15001 atgatgcttt aaataaggcg tatgatttgt ttagtatgat ggatagcgtc gataaacatt
    15061 tagctgttat agaagatgaa cgacgtttaa tggggcgtgt tttagaaggt ggctttattg
    15121 atatacctta tttaactggt cacgaatttg gcgcgcgttt gcctaatgta acagcgaaaa
    15181 ttaaagaagc aaatgaaatg atggtcgatg ccttagaaga tgctaaactt cagtataaaa
    15241 aatataaatc attaattgat aaagtgaaga gtgattactt ttcaagagaa gctgatgatt
    15301 tgaaagctga tatgcaacaa ttggcgccac gagtaaagta ccttgcgcgt attgtgaaag
    15361 atgttatgtc agaattcaat cgaaaaaagc gtagcaaaaa tattttggat ttttctgatt
    15421 atgaacattt tgcattacaa attttaacta atgaggatgg ttcgccttca gaaattgccg
    15481 aatcataccg tcaacacttc caagaaatat tggtcgatga gtatcaagat acgaaccgag
    15541 ttcaagagaa aatactatct tgcatcaaaa cgggtgatga acataatggt aatttattta
    15601 tggttggaga tgttaagcaa tccatttata aatttagaca agctgatcca agtttattta
    15661 ttgaaaagta tcaacgcttt actatagatg gagatggcac tggacgtcga attgatttgt
    15721 cgcaaaactt ccgttctcga aaagaagtac tgtcaacgac taactatata ttcaaacata
    15781 tgatggatga acaagtcggt gaagtaaaat atgatgaagc ggcacagttg tattatggtg
    15841 caccatatga tgaatcggac catccagtaa acttaaaagt ccttgttgaa gcggatcaag
    15901 aacatagtga tttaactggt agtgaacaag aagcgcattt tatagtagaa caagttaaag
    15961 atatcttaga acatcaaaaa gtttatgata tgaaaacagg aagctataga agtgcgacat
    16021 acaaggatat cgttattcta gaacgcagct ttggacaagc tcgcaattta caacaagcct
    16081 ttaaaaatga agatattcca ttccatgtga atagtcgtga aggttacttt gaacaaacag
    16141 aagtccgctt agtattatca tttttaagag cgatagataa tccattacaa gatatttatt
    16201 tagttgggtt aatgcgctcc gttatatatc agttcaaaga agacgaatta gctcaaatta
    16261 gaatattgag tccaaatgat gactacttct atcaatcgat tgtaaattac attaatgacg
    16321 aagcagcaga tgcaatttta gttgataaat taaaaatgtt tttatcagat attcaaagtt
    16381 accaacaata tagtaaagat catccggtgt atcagttaat tgataaattt tataatgatc
    16441 attatgttat tcaatacttt agtggactta ttggtggacg tggacgacgt gcaaatcttt
    16501 atggtttatt taataaagct atcgagtttg agaattcaag ttttagaggt ttatatcaat
    16561 ttattcgttt tatcgatgaa ttgattgaaa gaggcaaaga ttttggtgag gaaaatgtag
    16621 ttggtccaaa cgataatgtc gttagaatga tgacaattca tagtagtaaa ggtctagagt
    16681 ttccatttgt catttattct ggattgtcaa aagattttaa taaacgtgat ttgaaacaac
    16741 cagttatttt aaatcagcaa tttggtctcg gaatggatta ttttgatgtg gataaagaaa
    16801 tggcatttcc atctttagct tcggttgcat atagagctgt tgccgaaaaa gaacttgtgt
    16861 cagaagaaat gcgattagtc tatgtagcat taacaagagc gaaagaacaa ctttatttaa
    16921 ttggtagagt gaaaaatgat aaatcattac tagaactaga gcaattgtct atttctggtg
    16981 agcacattgc tgtcaatgaa cgattaactt caccaaatcc gttccatctt atttatagta
    17041 ttttatctaa acatcaatct gcgtcaattc cagatgattt aaaatttgaa aaagatatag
    17101 cacaaattga agatagtagt cgtccgaatg taaatatttc aattgtgtac tttgaagatg
    17161 tgtctacaga aaccatttta gataatgatg aatatcgttc ggttaatcaa ttagaaacta
    17221 tgcaaaatgg taatgaagat gttaaagcac aaattaaaca ccaacttgat tatcgatatc
    17281 catatgtaaa tgatactaaa aagccctcaa aacaatctgt ttctgaattg aaaagacaat
    17341 atgaaacaga agaaagtggc acaagttacg aacgagtaag gcaatatcgt atcggttttt
    17401 caacgtatga acgacctaaa tttctaagtg aacaaggtaa acgaaaagcg aatgaaattg
    17461 gtacgttaat gcatacagtg atgcaacatt taccattcaa aaaagaacgc atatctgaag
    17521 ttgagttaca tcagtatatc gatggattaa tcgataaaca tattatcgaa gcagatgcga
    17581 aaaaagatat ccgtatggat gaaataatga catttatcaa tagtgagtta tattcgatta
    17641 ttgctgaagc agagcaagtt tatcgtgaat taccgtttgt agttaaccaa gcattagttg
    17701 accaattgcc acaaggagac gaagacgtct caattattca aggtatgatt gacttaatct
    17761 ttgttaaaga tggtgtgcat tattttgtag actataaaac cgatgcattt aatcgtcgcc
    17821 gtgggatgac agatgaagaa attggtacac aattaaaaaa taaatataag atacagatga
    17881 aatattatca aaatacgctt caaacgattc ttaataaaga agttaaaggt tatttatact
    17941 tcttcaaatt tggtacattg caactatagt attttgattt tcaaaagaat aaaaaataat
    18001 ttcgattaag tgcaaagtcc ttgtagcaga atgaacacaa ctcattttca aaattgtctt
    18061 acttatttat ttgttatttg ataacgaaaa aagttataat gtgaattaag ataaagatga
    18121 ggagttgaga atgaatgaaa ttcttatcat tcaagtataa tgacaaaact tcatatggcg
    18181 ttaaagtaaa acgcgaagat gctgtatggg atttaacaca agtatttgct gactttgcag
    18241 aaggagattt ccatcctaaa acattgttag ctggtttaca acaaaatcat actttagatt
    18301 ttcaagaaca agtacgtaaa gcagttgtag cagcagaaga tagcggcaaa gctgaagact
    18361 ataaaatttc atttaatgac attgaatttt taccaccagt aacacctccg aataatgtga
    18421 ttgcttttgg tagaaattac aaagatcatg cgaacgaatt aaatcatgaa gtggaaaaat
    18481 tatatgtatt tacaaaagca gcgtcatctt taacaggaga taatgcaaca attccaaatc
    18541 ataaagatat tactgatcaa ttagattatg aaggtgaatt aggtattgtt attggtaagt
    18601 ctggtgaaaa gattccaaaa gcattagctt tagattatgt ttacggctat acaattatta
    18661 acgatatcac tgatcgcaaa gcacaaagtg aacaagatca agcattttta tcaaaaagtt
    18721 taactggcgg ttgcccaatg ggtccttata tcgttactaa agacgaacta ccattacctg
    18781 aaaatgtaaa tattgttaca aaagttaaca atgaaattag acaagatggt aacactggcg
    18841 aaatgattct taaaattgat gaattaatag aagaaatttc aaaatatgtt gcactacatc
    18901 cgggagatat tattgcaact ggtacaccag caggcgttgg tgcaggtatg caaccaccta
    18961 aatttttaca accaggtgat gaagttaaag tgactattga taatattgga acgctgacaa
    19021 cttatatcgc taaataatta tcatttaaaa agctaaccag tctttatata gattggttag
    19081 ttttttcttg cttttctaaa aaggtgttaa agataaatta tttataatgt taccattttg
    19141 agatgaaagt gaaatattga tattaagaag tagttgatta ttttacagca gattcacaat
    19201 attctaataa ggaataaaat gtcatgttct tcctctcaaa tatagaagtg tggtagaata
    19261 tatattcgtg tataatcaaa tcatgattaa attacaagca agtgggtatt aatcccaaga
    19321 agctttaatt taataatata aataattaaa gtggggggaa tcagtatgtt acatttacat
    19381 atattaagtt gggtattagc gattatttta tttatcgcta catacttaaa catttcaaaa
    19441 aatcaaggca gatcaccatt tttcaaaccg ttgcacatga ttttacgctt atttatgctg
    19501 ttgacgttaa tttcaggatt ttggatatta attcagtcat ttatgaatgg cggggcaaat
    19561 catatgttgc ttacattgaa aatgctgtgt ggtgttgcag tagttggatt gatggaagtg
    19621 tcgattgcta aaagaaagag acatgaacaa agtcacacaa tgttttggat aacaattgca
    19681 ttaattatca tcacaatggt attaggtgtc attctaccgt tagggcctat atcaaaatta
    19741 ttcggtattg gctaattgat aagacatata gtatatgaca taaggcatgg acaattatta
    19801 gtccaagacc ttatgtcttt tttgattcta aaaatgttaa acataaaaaa tggactggat
    19861 agtagtacca gtccaacata attctaacct agacaattaa gattagattg aatcgcatga
    19921 ttcatttatt tagctttgta accaatcata ttgattaaat ctttagggtg gctatatggt
    19981 ggtgcataag ccacttcaaa ctcagttaac tcatctacag ttagctggtt catcattgcc
    20041 atcgatagta catcaatacg tttatctgca ccttcttttc ctactgcagc tgctcttaaa
    20101 atctgacggt ttgaagtgtc ataatatact cttaagtgta aaggggaatt tcctgggtaa
    20161 taattcgcgt gtgcaccttg agtgacttct accattttat agtcaaattg ctttagttcg
    20221 tttggtttaa cgccgacact cgcaaatgta taatcaaaga acttcacaat attgttgcct
    20281 aagaagcctt tgaattcaat agtgtcattt ccagcaattt gttcggcaac aatacttgct
    20341 gcacggtgag cgccccaagc taaaggaaca ctagccggta gatcgacatg tcgataatgt
    20401 gatgttgcaa tatcgcctat tgcataaatg tttggaacat ttgtttcaaa tttatcgttt
    20461 accggtatga aaccttttcg atcaagtttg atatttgaac tttcgataaa ttttgaattg
    20521 gggtgagtac cgacaccttc aataatcata tcgtaatgtt caacttttcc tgatttaaat
    20581 gtaatttcat ttccattgat agcattaatt tcctcattta aacggtatgg aatctcccgc
    20641 ttatctaatt catcaagtat aggttgattc atgtcggcat ccattaattt atttatctta
    20701 tcagatcgat gaattaaagt agggtgtaaa ccacgttcat aaagattttc aagaacttct
    20761 aatgaaacat aacctgcacc tacaaccaat actttatcaa cttgatttgc tttgatgaat
    20821 tgatcgatag catcagtgtc ttctaaattt ctaagtgtaa atgtaatatc actttcaaag
    20881 ccaaggctat ttgcacttgc accagggctt aaaatgagtt tatcgtaaga ttcttcaaat
    20941 tgttcgtttg tctttctatt taatacagat acagtttgtc tttcatcatt gattgcaata
    21001 acttcatgat aagtttttac tgtaatttgc tttctatcat aaaatttttc aggtgtatac
    21061 gctaaagcat atcttctatc ttcaacaact tcgccaatga cataaggcaa tgcacaatta
    21121 gcaaagctca tatcacgatc tttttcaaaa ataataatgt cactttcttt atctaaacgt
    21181 cgaatttggc tggcacatgt tgcaccgcca gcgactgctc cgactacgac tattttgggc
    21241 atcgtaactt acctcctatt gttttaaaat attataaaat gattagttat aaattaacag
    21301 taatatctaa tatttaaatt aaagaaatca ttcaaatatc gaccaatgcc atcttcatta
    21361 ttgttgaatg taatattgtt cgctacatct ttaagttctt gcaaaccatt ttccatagca
    21421 acaccgtgac gcgcgtactc aatcatttca atatcattat cttcatcacc gaatgcaata
    21481 atattatttc ggtcaatatt taaaaattgt ctaacttgct caatgcctct tgctttatta
    21541 ataccaagtt ttacaatttc aatgacaggg aatggtgcgc cccagcgtcg atgctcaata
    21601 tgatcggcat aaaaatgagt aagcatattt ttgatttcag gtattttact ttcttcggct
    21661 tcaattaaaa ttgaggtagg ggattctttc aagtggacaa gtaaattacc agtttgaatt
    21721 cttggattac ccattgaaaa accttcaaat aatcttggat catgattgtt aatgaaaaca
    21781 taatctttca cttctgctat aatattcgat acttgatatt gttgtaatcc ttgaataatg
    21841 ttttgtgcga tgcctaaatc taaaatttca tggcaagttt tgaagttttt atctttaggg
    21901 tgatgtacgt aagcgccatt aaaattaaca attggtgtcg ttaaatttaa ttcatgataa
    21961 tacatttgac ttgcacgata aggtctgcca gtcgcaatca taatttggtg tccacgttgt
    22021 tgtaattcat ttaatacttg tttagtatat gatgaaattt ctttgttatc gtttaataat
    22081 gttccgtcta agtctagaca tattaaatgt ggttgcatat ctctctcctc ataagttgct
    22141 tagttcatat agatacatca tagcatttta ttggcgtttt gtcggatatt tgatatatta
    22201 tgaataatat gacattaaag aggtgatatg gatggaagag gcattgaaag atagtatctt
    22261 aggtgcatta gaaatggtaa ttgaccctga attaggaatt gatatcgtta atttgggttt
    22321 agtatacaaa gtgaatgttg atgatgaagg cgtatgtaca gttgatatga ctttaacatc
    22381 aatgggatgt ccaatgggac ctcaaattat tgatcaagtt aaaacagtat tagcagagat
    22441 tcctgaaatt caggatactg aagtgaatat cgtatggagt ccaccttgga caaaagatat
    22501 gatgtcacgt tacgctaaga ttgcacttgg tgtgagctaa cataaacttg cattgcctgt
    22561 agaatttctt ttcgaaattc tctgtgtttg ggcccgcagt caatataaca ttgtagagta
    22621 taattcattg tgaataagct cccttcaaag tagacattga aaaatgaaaa ttttgaaggg
    22681 agccttattt atgttctagt ttctgatttt attttgaaaa tttatatcta acttgtttca
    22741 aaaaatatga attaagacag tgacttgcac ttc
//
LOCUS       DAKTLH010000027        22165 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.027, whole genome shotgun sequence.
ACCESSION   DAKTLH010000027 DAKTLH010000000
VERSION     DAKTLH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22165
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.027"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            105..1013
                     /gene="rocF"
                     /locus_tag="O0394_000932"
     CDS             105..1013
                     /gene="rocF"
                     /locus_tag="O0394_000932"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HCY0499023.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
     gene            1202..2011
                     /locus_tag="O0394_000933"
     CDS             1202..2011
                     /locus_tag="O0394_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCY0499024.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            2013..2945
                     /locus_tag="O0394_000934"
     CDS             2013..2945
                     /locus_tag="O0394_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HCY0499025.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            2972..4327
                     /locus_tag="O0394_000935"
     CDS             2972..4327
                     /locus_tag="O0394_000935"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCY0499026.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIALDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            4604..12040
                     /locus_tag="O0394_000936"
                     /pseudo
     CDS             4604..12040
                     /locus_tag="O0394_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(12359..13465)
                     /locus_tag="O0394_000937"
     CDS             complement(12359..13465)
                     /locus_tag="O0394_000937"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCY0499027.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVEDSSIEAGLRRVLAETSQYITNEFDFTEAEQAAYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(13465..13899)
                     /locus_tag="O0394_000938"
     CDS             complement(13465..13899)
                     /locus_tag="O0394_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0499028.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            complement(13911..16043)
                     /locus_tag="O0394_000939"
     CDS             complement(13911..16043)
                     /locus_tag="O0394_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCY0499029.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLDQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMI
                     DKDDVHVETKDVDGNTSFENEQTSYLTSVFEKHLSDEKSEQLLHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFISDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            complement(16078..17616)
                     /locus_tag="O0394_000940"
     CDS             complement(16078..17616)
                     /locus_tag="O0394_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCY0499030.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            18297..20102
                     /gene="glmS"
                     /locus_tag="O0394_000941"
     CDS             18297..20102
                     /gene="glmS"
                     /locus_tag="O0394_000941"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCY0499031.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            20334..21116
                     /locus_tag="O0394_000942"
     CDS             20334..21116
                     /locus_tag="O0394_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0499032.1"
                     /translation="MLIQLDQIGRMKQGKTILKKISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATSGTVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDDEIRNEAHQLLKLVGMFAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPTLAMIYVTHFIEEITANFSKILLLK
                     DGQSIQQGAVEDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            21184..22041
                     /locus_tag="O0394_000943"
     CDS             21184..22041
                     /locus_tag="O0394_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0499033.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINQLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     AHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAEHFLAE"
BASE COUNT         8316 a         3521 c         3968 g         6360 t
ORIGIN      
        1 tttttatgaa gtaataagat aaacagatta tatgtgtagc tattgcttga taatggtaag
       61 cgcatacatt attctttaat tacatagagc aaagggggac gcttatgaca aagacaaaag
      121 caattgatat tataggtgca ccatcaacat ttggacaaag aaaattaggt gttgatttag
      181 gaccaacagc aattagatat gctggattaa tttcaagatt aaagcaatta gaccttgatg
      241 tatatgacaa gggggatatt aaggtacctg ctgtgaacat tgaaaaattt catagtgaac
      301 aaaaaggatt aagaaattat gatgaaatta tagatgttaa tcaaaaatta aataaagagg
      361 tttcagcatc aattgaaaat aacagatttc ctctagttct tggtggagat cattctattg
      421 cggtaggttc agtatcagca ataagtaaac attataataa tttaggtgtt atttggtatg
      481 atgcacatgg tgatttaaat atacctgaag agtcaccaag tggaaatatt catggtatgc
      541 ctctaaggat tttgacaggc gaaggtccca aagaactttt agaattaaat agtaatgtaa
      601 tcaagccaga aaacatcgta ctaattggta tgagagattt agataaaggt gaaagacaat
      661 ttatcaaaga tcataatatt aaaacattta ctatgtcaga tattgataaa ttggggataa
      721 aggaagtaat tgaaaataca atagaatatt tgaagtcacg caatgttgat ggcgttcatt
      781 tatctttaga tgttgatgct ttagatccgc ttgaaacgcc aggcactggt actagagttt
      841 tgggtggtct tagttataga gaaagccatt ttgcattgga attactgcat caatcacatt
      901 taatttcctc aatggattta gttgaagtaa atccattgat tgacagtaat aatcatactg
      961 ctgaacaagc ggtttcatta gttggaacat tttttggtga aactttatta taaataaatg
     1021 atttgtagtg tataaagtat attttgcttt ttgcactact ttttttaatt cactaaaatg
     1081 attaagagta gttataatct ttaaaataaa ttttttctat ttaaatatat gttcgtatga
     1141 cagtgatgta aatgattggt ataatgggta ttatggaaaa atattacccg gaggagatgt
     1201 tatggatttt tccaactttt ttcaaaacct cagtacgtta aaaattgtaa cgagtatcct
     1261 tgatttactg atagtttggt atgtacttta tcttctcatc acggtcttta agggaactaa
     1321 agcgatacaa ttacttaaag ggatattagt aattgttatt ggtcagcaga taagtatgat
     1381 attgaacttg actgcaacat ctaaattatt cgatatcgtt attcaatggg gggtattagc
     1441 tttaatagta atattccaac cagaaattag acgtgcgtta gaacaacttg gtagaggtag
     1501 ctttttaaaa cgctatactt ctaatacgta tagtaaagat gaagagaaat tgattcaatc
     1561 ggtttcaaag gctgtgcaat atatggctaa aagacgtata ggtgcattaa ttgtctttga
     1621 aaaagaaaca ggtcttcaag attatattga aacaggtatt gcaatggatt caaatatttc
     1681 gcaagaactt ttaattaatg tctttatacc taacacacct ttacatgatg gtgcaatgat
     1741 tattcaaggc acgaagattg cagcagcagc aagttatttg ccattgtctg atagtcctaa
     1801 gatatctaaa agtttgggta caagacatag agctgcggtt ggtatttcag aagtatctga
     1861 tgcatttacc gttattgtat ctgaagaaac tggtgatatt tcggtaacat ttgatggaaa
     1921 attacgacga gacatttcaa acgaaatttt tgaagagttg cttgctgaac attggtttgg
     1981 cacacgcttt caaaagaaag gtgtgaaata atatgctaga aagtaaatgg ggcttgagat
     2041 ttattgcctt tcttttggca ttgtttttct ttttatctgt taacaatgtt tttggaaata
     2101 tctttaacac tggtaatctt ggtcaaaagt ctagtaaaac gattcaagat gtacccgttg
     2161 aaattcttta taacactaaa gatttgcatt taacaaaagc gcctgaaaca gttaatgtga
     2221 ctatttcagg accacaatca aagataataa aaattgaaaa tccagaagat ttaagagtag
     2281 tgattgattt atcaaatgct aaagctggaa aatatcaaga gaagtatcaa gttaaagggt
     2341 tagctgatga cattcattat tctgtaaaac ctaaattagc aaatattacg cttgaaaaca
     2401 aagtaactaa aaagatgaca gttcaacctg atgtaagtca gagtgatatt gatccacttt
     2461 ataaaattac aaagcaagaa gtttcaccac aaacagttaa agtaacaggt ggagaagaac
     2521 aattgaatga tatcgcttat ttaaaagcca cttttaaaac taataaaaag attaatggtg
     2581 acacaaaaga tgtcgcagaa gtaacggctt ttgataaaaa actgaataaa ttaaatgtat
     2641 cgattcaacc taatgaagtg aatttacaag ttaaagtaga gccttttagc aaaaaggtta
     2701 aagtaaatgt taaacagaaa ggtagtttag cagatgataa agagttaagt tcgattgatt
     2761 tagaagataa agaaattgaa atcttcggta gtcgagatga cttacaaaat ataagcgaag
     2821 ttgatgcaga agtagattta gatggtattt cagaatcaac tgaaaagact gtaaaaatca
     2881 atttaccaga acatgtcact aaagcacaac caagtgaaac gaaggcttat ataaatgtaa
     2941 aataaatagc taaattaaag gagagtaaac aatgggaaaa tattttggta cagacggagt
     3001 aagaggtgtc gcaaaccaag aactaacacc tgaattggca tttaaattag gaagatacgg
     3061 tggctatgtt ctagcacata ataaaggtga aaaacaccca cgtgtacttg taggtcgcga
     3121 tactagagtt tcaggtgaaa tgttagaatc agcattaata gctggtttga tttcaattgg
     3181 tgcagaagtg atgcgattag gtattatttc aacaccaggt gttgcatatt taacacgcga
     3241 tatgggtgca gagttaggtg taatgatttc agcctctcat aatccagttg cagataatgg
     3301 tattaaattc tttggatcag atggttttaa actatcagat gaacaagaaa atgaaattga
     3361 agcattattg gatcaagaaa acccagaatt accaagacca gttggcaatg atattgtaca
     3421 ttattcagat tactttgaag gggcacaaaa atatttgagc tatttaaaat caacagtaga
     3481 tgttaacttt gaaggtttga aaattgcttt agatggtgca aatggttcaa catcatcact
     3541 agcgccattc ttatttggtg acttagaagc agatactgaa acaattggat gtagtcctga
     3601 tggatataat atcaatgaga aatgtggctc tacacatcct gaaaaattag ctgaaaaagt
     3661 agttgaaact gaaagtgatt ttgggttagc atttgacggc gatggagaca gaatcatagc
     3721 agtagatgag aatggtcaaa tcgttgacgg tgaccaaatt atgtttatta ttggtcaaga
     3781 aatgcataaa aatcaagaat tgaataatga catgattgtt tctactgtta tgagtaattt
     3841 aggtttttac aaagcgcttg aacaagaagg aattaaatct aataaaacta aagttggcga
     3901 cagatatgta gtagaagaaa tgcgtcgcgg taattataac ttaggtggag aacaatctgg
     3961 acatatcgtt atgatggatt acaatacaac tggtgatggt ttattaactg gtattcaatt
     4021 agcttctgta ataaaaatga ctggtaaatc actaagtgaa ttagctggac aaatgaaaaa
     4081 atatccacaa tcattaatta acgtacgcgt aacagataaa tatcgtgttg aagaaaatgt
     4141 tgacgttaaa gaagttatga ctaaagtaga agtagaaatg aatggagaag gtcgaatttt
     4201 agtaagacct tctggaacag aaccattagt tcgtgtcatg gttgaagcag caactgatga
     4261 agatgctgaa agatttgcac aacaaatagc tgatgtggtt caagataaaa tgggattaga
     4321 taaataaata ctgtattaca aatgagccga tgcgtatgca tcggtttttt gtgtttgtag
     4381 aaataattta tagtacaaac gtaaaatgat ataaacaaaa taaaaacaaa gtaatcaata
     4441 tgtaatataa aatacactgg tactcaatat ataatgatga taaaattaat tttaattaga
     4501 tagagttgct ttgtgttttt aacgcagatg ctactactta tcttaacagt tgattaagtg
     4561 aaatcattta acagcgagaa taatcaacca ggaggatgac ttaatgaatt tattcagaca
     4621 acaaaaattt agtatcagaa aatttaatgt cggtattttt tcagctttaa ttgccactgt
     4681 tacttttata tctactaacc cgacaacagc gtctgcagca gagcaaaatc agcctgcaca
     4741 aaatcaacca gcacaaccag ctgatgccaa tacacagcct aacgcaaatg ctggtgctca
     4801 agctaatcct acagcacagc cagctgcacc tgccaaccaa ggacaaccag cagtacaacc
     4861 agcaaaccaa ggtggacagg ctaatccagc aggaggagca gcacaaccaa atacacaacc
     4921 agctggacaa ggtaatcaag ctgatccgaa taacgctgca caagcacaac ctggaaatca
     4981 agcaacaccg gcaaaccaag caggtcaagg aaataaccaa gcaacaccta ataataatgc
     5041 aacaccggca aatcaaacac agccagcgaa tgctccagca gcagcgcaac cagcagcacc
     5101 tgtagcagca aacgcacaaa ctcaagatcc aaatgctagc aatactggtg aaggcagtat
     5161 taatacgaca ttaacatttg atgatcctgc catatcaaca gatgagaata gacaagatcc
     5221 aactgtaact gttacagata aagtaaatgg ttattcatta attaacaacg gtaagattgg
     5281 tttcgttaac tcagaattaa gacgaagcga tatgtttgat aagaataacc ctcaaaacta
     5341 tcaagctaaa ggaaacgtgg ctgcattagg tcgtgtgaat gcaaatgatt ctacagatca
     5401 tggtaacttt aacggtattt caaaaactgt aaatgtaaaa ccagattcag aattaattat
     5461 taactttact actatgcaaa cgaatagtaa gcaaggtgca acaaatttag ttattaaaga
     5521 tgctaagaaa aatactgaat tagcaactgt aaatgttgct aagactggta ctgcacattt
     5581 atttaaagta ccaactgatg ctgatcgttt agatttacaa tttattcctg acaatacagc
     5641 agttgctgat gcttcaagaa ttacaacaaa taaagatggt tataaatact attcattcat
     5701 tgataatgta ggtctattct caggatcaca tttatatgtc aaaaatagag atttagcacc
     5761 gaaagcaact aacaataaag aatatactat taatactgaa atcggtaaca atggtaattt
     5821 tggtgcttca ttaaaagcag atcaatttaa atatgaagta acattaccac aaggtgtaac
     5881 ttacgttaat aattcattaa ctacaacatt ccctaatggt aatgaagaca gtacagtatt
     5941 gaaaaatatg actgttaatt atgatcaaaa tgcaaataaa gttacattta caagccaagg
     6001 tgtgacaacg gcacgtggta cacacactaa agaagtttta ttcccagata aatctttaaa
     6061 attatcatat aaagttaatg ttgcgaatat cgatacacct aaaaatattg attttaatga
     6121 aaaattaaca tatcgtactg cttcagatgt tgtaattaat aatgcgcaac cagaagttac
     6181 actaactgca gatccatttt cagtagcggt tgaaatgaac aaagatgcgt tgcaacaaca
     6241 agtaaactca caagttgata atagtcatta cacaacagca tcaattgcag aatacaataa
     6301 acttaaacaa caagcagata ctattttaaa tgaagatgcg aatcatgtta aaactgcaaa
     6361 tcgtgcatct caagcggata ttgatggttt agtaactaaa ttacaagctg cattaattga
     6421 taatcaagca gcaattgctg aattagatac taaagctcaa gaaaaggtta cagcagcaca
     6481 acaaagtaaa aaagttacgc aagatgaagt tgcagcactt gtaactaaaa ttaacaatga
     6541 taaaaataat gcaatcgctg aaattaataa acaaactaca gcacaaggtg tcacaactga
     6601 aaaagataat ggtatcgcag tgttagaaca agatgtgatt acaccaacag ttaaacctca
     6661 agcgaaacaa gatattatcc aagcagttac aactcgtaaa caacaaatta aaaagtcaaa
     6721 tgcatcatta caagatgaaa aagatgtagc aaatgataaa attggtaaaa ttgaaacaaa
     6781 ggcaattaaa gatattgatg cagcaacaac aaatgcacaa gtagaagcca ttaaaacaaa
     6841 agcaatcaat gatattaatc aaactacacc tgctacaaca gctaaagcag cagctcttga
     6901 agaatttgac gaagttgttc aagcacaaat tgatcaagca cctttaaatc ctgatacaac
     6961 aaatgaagaa gtagcggaag ctattgaacg tattaatgca gctaaagttt ctggtgttaa
     7021 agcaattgaa gcgacaacga ctgcacaaga tttagaaaga gttaaaaacg aagaaatctc
     7081 aaaaattgaa aatattactg actctacgca aacaaaaatg gatgcctata atgaagttaa
     7141 acaagctgca acagctagaa aagctcaaaa tgctacagtt tcaaatgcaa caaatgaaga
     7201 agtagcagaa gctgatgcag cagtagatgc agctcaaaag caaggtttac atgacatcca
     7261 agttgttaaa tcaaaacagg aagttgctga tacaaaatca aaagtattag ataaaatcaa
     7321 tgcaattcaa acacaagcaa aagttaaacc tgcagctgat acggaagtag aaaacgcata
     7381 taatacacgt aaacaagaaa ttcaaaatag caatgcttca actacagaag aaaaacaagc
     7441 tgcatataca gaattagata ctaaaaagca agaagcaaga acaaatcttg atgctgcaaa
     7501 tacaaacagt gatgtaacaa cagctaaaga caatagtatt gctgcaatta atcaagttca
     7561 agctgccaca actaagaaat cggatgcaaa agcggaaatc gctcaaaaag caagtgaacg
     7621 taaaacagca attgaagcaa tgaatgattc gactactgaa gaacaacaag cagcgaaaga
     7681 caaagtggat caagcagtag ttactgcaaa cgctgatata gataatgctg cagcaaacaa
     7741 tgatgtggat aatgcaaaaa ctacaaatga agctacaatc gcagccatta cacctgatgc
     7801 aaatgttaaa ccagcagcaa aacaagcaat tgcagataaa gtacaagctc aagaaacagc
     7861 aattgatgga aataacggct caacaactga agaaaaagca gctgctaaat aacaagttca
     7921 aactgaaaaa acaacagctg atgccgcaat agatgcagca catacaaatg cggaagttga
     7981 agcggctaaa aaagcagcaa ttgctaaaat tgaagcgatt cagccagcaa caacaactaa
     8041 agataatgcg aaagaagcaa ttgctacgaa agcgaatgaa cgtaaaacag caatcgctca
     8101 aacgcaagac attactgctg aagaaattgc agcggctaat gcggacgtag ataatgctgt
     8161 gacacaagca aatagcaaca ttgaagctgc taatagtcaa aatgatgtag accaagcgaa
     8221 aacgacaggt gaaaatagta ttgatcaagt aacaccaaca gttaataaaa aagcaactgc
     8281 acgtaatgaa atcacagcaa ttttaaataa caaattgcaa gagattcaag ctacgccaga
     8341 tgcaacagat gaagaaaaac aagcagctga tgctgaagca aatactgaaa atggtaaagc
     8401 aaatcaagcc atttcagcag caactactaa cgcacaagtt gatgaagcta aagcaaatgc
     8461 agaagcagcg attaatgcgg taacaccaaa agttgtgaag aaacaagcgg ctaaagatga
     8521 aattgatcaa ttacaagcaa cgcaaacaaa tgttatcaat aatgatcaga acgctacaac
     8581 agaagaaaaa gaagcagcta ttcaacaatt agcaacagca gttacagacg cgaaaaataa
     8641 tattacagct gcaactgatg ataatggtgt agatcaggcg aaagacgctg gaaagaattc
     8701 aattcaaagc acgcaaccag caacagcggt taaatcaaat gctaaaaatg atgttgatca
     8761 agctgtgaca actcaaaatc aagcaattga taatacaact ggtgctacaa ctgaagagaa
     8821 aaatgcagca aaagatttag ttttaaaagc taaagaaaaa gcgtatcaag atatcttaaa
     8881 tgcacaaaca actaatgatg ttacgcaaat taaagatcaa gcagttgctg atattcaagg
     8941 tattactgca gatacaacaa ttaaagatgt tgcgaaagat gaattagcaa caaaagcaaa
     9001 cgaacaaaaa gcgcttattg cacaaactgc agatgcgact actgaagaaa aagaacaagc
     9061 aaatcaacaa gtagacgcac aattaacaca aggtaatcaa aatattgaaa atgcacagtc
     9121 aatcgatgat gtaaacactg caaaagataa tgcaattcaa gcaattgacc caattcaagc
     9181 atcaacagat gttaaaacga atgcaagagc ggaattgcta actgaaatgc aaaataaaat
     9241 aactgaaata cttaataata atgagactac taatgaagaa aaaggtaacg atattggacc
     9301 agttagagca gcatatgaag aaggtttaaa taatattaat gcagcaacta ctacaggtga
     9361 tgtaactact gctaaagata cagcagtaca aaaagttcaa caacttcatg caaatcctgt
     9421 taagaaacca gcaggtaaaa aagaattaga tcaagctgca gctgataaga aaacacaaat
     9481 agaacaaaca ccaaatgcat cacaacaaga aattaatgat gcaaaacaag aagttgatac
     9541 tgaattaaat caagcgaaaa caaatgtcga tcaatcatca acaaatgaat atgttgataa
     9601 tgcagttaaa gaaggaaaag ctaaaattaa tgcagttaaa acatttagtg agtacaaaaa
     9661 agatgcttta gctaaaattg aagatgcata taatgctaaa gtaaacgaag cggataactc
     9721 taacgcatcg acttcaagtg aaattgctga agcgaaacaa aaacttgctg aattaaaaca
     9781 aactgcggat caaaatgtta atcaagctac ttctaaagat gacattgaag ttcaaattca
     9841 taatgactta gataatatta acgattacac aattccaaca ggtaaaaaag aatcagctac
     9901 aacagattta tatgcttatg cagatcagaa gaaaaataat atttcagctg acactaatgc
     9961 aacacaagat gaaaagcaac aagcaattaa gcaagttgac caaaatgttc aaactgcatt
    10021 agaaagcatt aataatggtg tggataatgg tgacgttgat gatgcattaa cacaaggtaa
    10081 agcagcaatt gatgctattc aagtagatgc tactgttaaa cctaaagcga accaagctat
    10141 tgaagttaaa gcagaagata cgaaagaatc tattgatcaa agtgaccagt taactgctga
    10201 agaaaaaact gaagcattag caatgattaa acaaattaca gatcaagcta aacaaggtat
    10261 tactgatgca acaacaactg ctgaagttga aaaagcgaaa gctcaaggac ttgaagcatt
    10321 tgataacatt caaatcgact caacagaaaa acaaaaagct atcgaagaat tagaaactgc
    10381 actagaccag attgaagcag gtgtaaatgt caacgctgat gctacaactg aagaaaaaga
    10441 agcgtttacg aatgctttag aagacatttt atcaaaagca actgaagata tttctgatca
    10501 aactacaaat gcagaaatcg ctactgtcaa aaatagtgcg cttgaacaac ttaaagcaca
    10561 acgtattaat cctgaagtta agaaaaatgc tttggaagca atcagagaag tggttaacaa
    10621 gcaaatagaa ataattaaaa atgcagatgc agatgcatcg gcgaaagaaa ttgcacgtac
    10681 ggatttaggt agatattttg acagatttgc tgataaatta gataaaacac aaacaaatgc
    10741 agaagttgct gaattacaaa atgtaacgat acctgcaatt gaagcgattg tgcctcagaa
    10801 tgatccagat gctaatgata ctaataatgg tatagataat aatgatgcaa cagcaaattc
    10861 aaatgctaat gcaacaccag aaaatactgg acaacctaac gtatctgaga cgacagctaa
    10921 tggcaaagca gacgcttctc cgacaacacc aaataatagt gatgcggcaa caggtgaaac
    10981 aacagcaaca tcagcaactg atgatgcaaa tgataaaccg caagctaata acaattctag
    11041 tgtagatgca tcaacaaaca gtccgacaat ggataacgat gtaactagta aaccagaagt
    11101 agaatcaact aataacggca caactgataa acctgtaaca gaaactgata atgcaacgcc
    11161 agcagaaagc acaacaaata acaatagtac aactacagca acaaacgaga atgcaccaac
    11221 aggatcaaca gcaacggctc caacaactgc gagcacagaa gcagcatcat ctgctgatag
    11281 caaagataat gcatctgtaa atgattcaaa acaaaatgct gaagtaaata atagtgctga
    11341 atctcaatca actaatgaca aggttgcaca accaaaatct gaaaataaag ctaaggcaga
    11401 aaaagatggt agtgattcaa caaatcaaag tatggttgaa tcaacaactg aaacattgcc
    11461 ttcagcagac ataacagaac caaatgtacc ttctaataca tcaaaagata aagaagaaag
    11521 tacgacaaat caaactgatg caggacaact taagtctgaa actaacgttg catctaacga
    11581 agcagataaa tcgccaagca aagctgatac tgaagtttcg aataaaccat caacatctgc
    11641 atcttctgaa gcaaaagaaa aaatgacttc aactaatgtt agccaaaaag atgatacggc
    11701 aacagcagat actaatgata cgcaaaaatc agttggttca gctgcaaaca ataaagctac
    11761 gcaaaacgac ggtgccaatg catctccagc tacagtttca aatggaagca atagtgctaa
    11821 tcaagatatg cttaatgtaa ctaacactga cgaccaccaa gctaagacaa aatcagctca
    11881 acaaggaaaa gttaataaag ctaaacaaca agctaaaact ttaccagata ctggtatgtc
    11941 acataatgac gatttaccat atgctgaatt agctctaggt gcaggtatgg cattcttaat
    12001 tagaagattc actaagaaag accaacaaac tgaagaataa aatttattca agaatgtgaa
    12061 catctagtta tttcataggg aaatattcct tactaagtaa ccaagtctaa cagttaatat
    12121 gaatttgttt tagtaatgac caatagccat tgaataggtg gctgtggctc gaaaataaaa
    12181 caaattatta acgcaaaaat actcctttca tacataacac aagttaagtg tctatgttga
    12241 agggagtatt ttttatgttc agaaatttaa tattgttaat tcaagggaac tactacatta
    12301 ttactgattg ttaacgaata acgttggaaa cttcttgttt caaacaaagc tcaataaatt
    12361 atgaaagtga attgtacgct tcaattattt catcggctaa gccatcgtca actttagcat
    12421 attcacttaa aaacgctttt aaaccgtgtt ctttaatgta attatttttc tcaacagttt
    12481 cttggtctgc tgtatcatca tacttcaata acaatgcagc tgtatttaat aaaccagtgc
    12541 gttctaaatc tttattataa agatatgtta atggttttat aattctatct ttagggccaa
    12601 ttttacgtaa tgtacctcgt ccgacacgtg ttacttcatc agataaataa gaattgttga
    12661 aacgatctat tattttttca acataagcag cttgttccgc ttcagtaaaa tcaaattcat
    12721 tagtaatata ttgactagtt tcagctaaaa cacggcgtaa gccagcttca attgaactat
    12781 cttcaactgc atctaaaact gtagctttac ctgcaaattt accagcatac gctaaatatg
    12841 catgtcctgt attcacagtt aataatttac gctcaatata tggtgttaaa tcatcaacat
    12901 atttaatatg gtttagttct ggaccatacc atgcatcttt ttcaacaacc cattcgtaaa
    12961 atggctcaac cataacgtct aatatatttt cattcttttg taatggtaca attctatcaa
    13021 ctgctgagtt agcaaaatga atattgttac caagagggcc agtaatatca agtactgctt
    13081 ttttcaatgt atcagttgcc ataatagcat tctcacaagc aacaatatta acatggtttg
    13141 ttttttcttt taagaaaggc gcaaaagatt tagcaataat aggtagtatg ttaacaccaa
    13201 cagctgttgt aataatatca gcttctagaa ttgcttgttt caacgcttca gaaggttgca
    13261 ttgaattaat tgcatcaaca ttattcacgc gcgtcgttgt tttagactca tcagctaaaa
    13321 taacatcgta ttgatgatca tgagctaaag cattaatgat ttcttcattg acgtctgcaa
    13381 atgttacttt aacattgttg tctgcaagaa tataaccaat gaaaccacga cctatgttac
    13441 cagcaccaaa gtgaactgct ttcattatgc atctgcctcc tcgaatactt gtttaatttc
    13501 ttctgcagat tttgcttgaa cgatacgatc cacattttct tcttcgctaa atgtaattgc
    13561 aattttagat aacaagtcta aatgttcgcc atctttacca gcaattccca cgacaacttt
    13621 aactacttcg ccatcccagt caacgccttc agggatttgt aataatgtta aacctgagtg
    13681 aatcacattt gttttagctt catctgtgcc atgaggaatt gctaagccat ttcccataaa
    13741 tgttgatacg acttgctcac gatctttcat tgcttgaata taagcatctg ttacagcacc
    13801 actatcaact aaggctttac ctgctttttc aattgcttca ttttggctgt taacatttac
    13861 atttaaaaag atattgtcat tactaaataa ttcgctcatt ttgtacactc ctaaataaat
    13921 tgttttttaa ttcgttctaa aaatttgttt cttaataatg tttcaagttc ctgtggattt
    13981 tccataaaag tatcaataga ttctaatttc aaacttaaaa attcggacaa gtcacttacc
    14041 agtgatgcca taaaatcatt gtcagaaata aacatggaaa tcatatattt aattgtttga
    14101 ttgtcatttt gaatactagg caatgttaac ggttcttcta aaactgtgat tagaatcata
    14161 gggtgtttaa tcatattgtc tcttaggtgt ggtattgcaa caggatatgg acttaatatc
    14221 catcctggat tgtcaatcaa tcgttgctct agtagttcag cgaatgacgt tggatcgttt
    14281 attacatcgc attgatatag ataatctgcg atatatgttt gccactgttt aacttcggta
    14341 ctaactattt tcactgaatc aagcaaagtt aaacccgaac gcatatgatg caataattgt
    14401 tctgattttt cgtcacttaa atgtttttcg aaaactgaag ttaagtatga agtttgttca
    14461 ttttcaaaag atgtgttgcc atcaacatct ttcgtttcaa catgaacatc atccttatca
    14521 atcatgtttg atgatttatc atgcgtctct tggaacgtag actttgtatt taaaaactgt
    14581 gcgacataac tgatatcact atctggtaat aatgggttta ccgttaaata gggggagtcg
    14641 atgtctaaat tcacagtaga aataatgcca tcatattgac ttaaatctag tgacttcaaa
    14701 tcgctgactg atgcttgtgt aatacgctca atctcactaa aaacttgatc tagacgagtt
    14761 gataatagac gactagttcc cataccactg ctgcaaacga ctaatatgtt taaaaatcga
    14821 ttaccttggt ttttaatcga gccaccaaag tgtaaaacta taaaagcaat ttcactctct
    14881 ggaaaaatca aatcaggcca acaatcattt aaagctaatc ttacattttc aaatagtctt
    14941 ggatatttga acttaatcat gtctgttaac ggattatagg tctcgatatt agcttgcaaa
    15001 cgattgattg caggattaag gtgtagcgtc agtccatcta ctaaagtatc caaatctgca
    15061 aatgtcatac cggaaatcat ttctactttg ttaacaaacg ctttaatttt gttttcttca
    15121 caacgattat ttaatgatgt atcattaaga ttttttcgtt tagctccacg taaatggata
    15181 gtaatgaaag caatttctgc ctgattaaac gtgacgtcat atatttgacc aagtttatca
    15241 gcaagttcgc ttgctacttt gtgttcaaat gtgttttgta cagaatcata aatatcatcg
    15301 tttaatgcga catactctcc attttttata cgtgaaatgg agagcacgat atggacagtt
    15361 aaagttaaat aacttgattc ggtaagttgg tagggtaagg cacttaggta gtccattaat
    15421 agtctttcaa cattaaaaat gcgctctaag tcaacaaagt ctttgtggat ttgatttaat
    15481 gaatgaaaga caaaatgatt ttcaattact gaataaacgc tagtactatt taagttattc
    15541 accattaatt gacttaaaaa ttcacgtttc tttgattcag tacctaccaa gtaaatgcct
    15601 tcgccacgct ttcgagataa agatagttgg tacttattta aatcaagctc taaatcatct
    15661 aacatctttg ctaaagtctg gacagaaacg ccaatttctt gcgctaaact atattgttta
    15721 actggctcct tggcttgtat caaagcgtat ataataatta ctttctgctc ttcaacagat
    15781 aagtcaatgg tttgatgttg tgcaatcgat tgcttcaaat cgtttaaatc agaatctgtg
    15841 ccagcaatgc gtagcccttt tttgtttgcg cgttctaaag ttaatgaaaa tgaagttaaa
    15901 tatgcttcaa caccttttaa ttcacggtga atagtacgag aggacaccgc aagttgctga
    15961 gcaatgtcgt gtatagtgat atattgaccg tggtacttaa tcaatagggc tatcatttct
    16021 ttttcacgtg tactcaataa catggcggta tcctccattt tttaatttat ttaattatta
    16081 tgcttgatca tcttttttta gattatttaa aagttcttca tatcttggtg aattaaggaa
    16141 attatccact gaaatatgga tggcatttgg tgtttgttta atagcacgat cagttagttt
    16201 tttctgagtg ataactaatt gagcatcttt tggcaattga ttaatcgcag tatttgtaac
    16261 tgtaatatca ttaatgcccg cctttttaaa tttattacgt aacatgcttg cacccattgc
    16321 actagaaccc atacccgcat cgcaggcaaa aataacatga tttatattat tgtaattgtg
    16381 tgcatcaaca tcttcagtgt tgtaattatc caatagcgct tcaggatcat catctgatga
    16441 agatgtttca ctaacattac cactagcatt ttcttctgta ttaacatttt tatcagaaga
    16501 tactaactta gaagcaacgc ttgatttttt ccctttagta ttttccattt gagctgtcgc
    16561 agcttctaaa tcctgctttg gttctctagt gaacttcata attaaagcag ctacaacgaa
    16621 tgatacaagt gcagcaagga agacaccgag taacatgtgc aagaattcac ctctaggtgc
    16681 atttaaacag taaactataa atgaacctgg tgacgcggga cttttaaatc caaatcctgt
    16741 tgcttgataa gttgcaacac cagtcattcc acctaaaata acagcgataa ataataaagg
    16801 acgcattaat acatatggga aataaatttc atgaatacca cctaagaagt ggataattcc
    16861 agcaccatat gacgttgctt ttgcagtgcc ttttccaaaa atcatataag caagtaagat
    16921 acctaaacct ggtccagggt tagattcaat tgtgtataaa attgattgac cagcttttgc
    16981 agcttgatct gcaccaagcg gtgtgaatac accatggtta atcgcattgt ttaaaaatac
    17041 aatttttgca ggctctacta aaatacttac aagtggaagt aggtgtgcat gtactaatgc
    17101 ttcaactgcc actgataaaa tatgcataat aaatttcata agtggtgcta aaattttaaa
    17161 tcctgcaatc gtcatgataa atcctaaaat accagcagaa aagttattaa ataacatttc
    17221 aaaaccttgc ggcgttctag gttgaatcaa ttggtcggtc ttcttcatta accaaccaac
    17281 aagtggaccc ataatcattg caccaagtaa cattggtgta tcaggtaatg caacgatgac
    17341 ccccatagtt gctgttgctg cgatgatacc accacgtaaa tcataaatta aacgaccacc
    17401 actaaatgcg atcaataatg ggattaaata agtaatcatt ggtcctgcta aagtagctaa
    17461 atctttgtta ggtaaccatc cattatctat aaaaatggcc gcgataaaac cccaagcgat
    17521 gaaagcgcca atgtttggca tgatcatact acttaagaat gatccaaatg cttgaacacg
    17581 acgaccaatt ccttttttct cttcagtttg tgacatgttt acacctcttt tttaatatat
    17641 gtgactatat atagtgtata gagaggttac ttgttactca atataaacaa aaatcaactt
    17701 tgtcaaaata aatgtgacaa aattaaataa agtgtcatca atgtgacagt atagatattt
    17761 tgaaaaagta aaacaaaaaa attgttttag gatttttaaa attttattgt gaaaatattt
    17821 gcaaaacaaa acaacaccgt tgtacaataa tgattaatgg aaagggggaa agttcggcag
    17881 tacagttaaa gcgcctgtgc aaataaatat ttgtatttga agattaaagg ttaatatatg
    17941 agtggccttt atagagtgca atatatgtat ttgtagacga ggaggatagt gatcgaatag
    18001 atcggcggat gctatcccgg atgtggctca ttcgttagct tattaagtaa aacattaggg
    18061 tgacttaatg gacaaagtta ataagatcgc cagaaattga atataaaaaa tattaatatg
    18121 gaaagtacag tgtgagcaat ttgtatagtt gtaaaaataa ctatgcttaa tttgttatgg
    18181 atgaatgcga tgatagcatg ttcctattta tattatgaaa gcagattgtc aatctaaatt
    18241 atcggcaata aatcataatt tacgcgtact attccaatat ggaggaaaat gtcgttatgt
    18301 gtggaattgt tggttatatt ggctatgata atgccaaaga attattatta aaaggtttag
    18361 aaaaattaga atacagaggt tatgactctg caggtatcgc agtagtaaat gatgataata
    18421 caactgtatt taaagaaaaa ggtcgtattg cagaattacg taaagttgct gatagtagcg
    18481 attttgatgg acctgttgga atcggtcaca cacgttgggc aacacacggt gtaccgaatc
    18541 atgaaaactc tcatccacat caatcatcaa atggccgttt tactctagtt cataacggtg
    18601 ttattgaaaa ctatgaagag ttaaaaggtg aatacttaca aggtgtatca ttcatttcag
    18661 aaacagatac agaagttatc gttcaattag ttgaatactt ttcaaatcaa ggactttcaa
    18721 ctgaagaagc atttacaaaa gttgtgtcat tattacatgg ttcatatgca ttaggtttat
    18781 tagatgctga agacaaagac acaatctatg ttgctaaaaa taaatcacca ttattattag
    18841 gtgttggtga aggtttcaat gttatcgcat cagacgcact tgcaatgtta caagtgacaa
    18901 gcgaatataa agaaatccat gaccatgaaa tcgttattgt taaaaaagat gaagttatta
    18961 ttaaagatgc agatggaaac gttgtagaac gtgattcata tattgctgaa attgatgcat
    19021 cagatgctga aaaaggtgtt tatgcacact acatgttaaa agaaattcat gaacaaccag
    19081 cagtaatgcg tcgtattatt caagaatatc aagatgcaga aggtaacttg aaaattgatc
    19141 aagacatcat caatgatgtt aaagaagcag accgcattta cgttattgca gcaggtacaa
    19201 gctaccatgc aggtttagta ggtaaagaat ttttagaaaa atgggctggc gtaccaactg
    19261 aagtacacgt tgcatcagag tttgtctaca acatgccatt attatctgaa aaaccattgt
    19321 tcgtttatat ttctcaatca ggtgaaactg cagatagccg cgccgtatta gttgaaacta
    19381 ataaattagg tcataaatca ttaacaatca ctaatgttgc aggttcaact ttatcacgtg
    19441 aagcagacca cacattgtta ttacacgcgg gtcctgaaat cgcagttgca tctacaaaag
    19501 catatactgc acaaattgca gtattatcaa tcttgtctca aatcgttgca aaagagcatg
    19561 gtcgtgaagc agatattgat ttattgagag aattagcaaa agtaacaaca gcaatagaag
    19621 caattgttga cgatgcacca attatggaac aaattgctac agatttctta gaaacaacac
    19681 gcaatgcatt ctttatcgga cgtactattg actataacgt aagtttagaa ggtgcgttaa
    19741 aacttaaaga aatttcttac attcaagcag aaggttttgc tggtggagaa cttaaacatg
    19801 gtacaattgc cttaatcgaa gaaggtacac cagttgtagg tttagcaaca caagagaaag
    19861 ttaatttatc aattcgtggt aacgttaaag aggtagtagc acgtggtgca catccatgta
    19921 ttatttctat ggagggtctt gaaaaagaag gcgacactta tgtcattcct catgtacatg
    19981 aattgttaac gccattagta tcagtggttg cattacaatt aatttcatac tatgcagcat
    20041 tacacagaga tttagatgtt gataaaccac gtaaccttgc taaatcagtt actgtggaat
    20101 aattcacttt tttagaatca atcatgtatt aaaattaaag tatatggcac ccttttagat
    20161 taatcgacta gaagggtgct tttttaggtc gacttagctt ttacttcatc ttaatttggc
    20221 agaaatgcgt aaaaatgaag tgttttattt atttaaatag tctgacaatt aagggtgtta
    20281 tgttaatatg attttatgag aagtatggag tagcaataaa ggggtgacct cgcatgttaa
    20341 ttcaattaga tcaaattggg cgaatgaagc aaggaaaaac aattttaaaa aagatttctt
    20401 ggcaaattgc taaaggtgat aaatggatat tatatgggtt gaatggtgct ggcaagacaa
    20461 cacttctaaa tattttaaat gcgtatgagc ctgcaacatc tggaactgtt aaccttttcg
    20521 gtaaaatgcc aggcaaggta gggtattctg cagagactgt acgacaacat ataggttttg
    20581 tatctcatag tttactggaa aagtttcaag agggtgaaag agtaatcgat gtggtgataa
    20641 gcggtgcctt taaatcaatt ggtgtttatc aagatattga tgatgagata cgtaatgaag
    20701 cacatcaatt acttaaatta gttggaatgt ttgctaaagc gcaacaatat attggttatt
    20761 tatctaccgg tgaaaaacaa cgagtgatga ttgcacgagc tttaatgggg caaccccagg
    20821 ttttaatttt agatgagcca gcagctggtt tagactttat tgcacgagaa tcgttgttaa
    20881 gtatacttga ctcattgtca gattcatatc caacgcttgc gatgatttat gtgacgcact
    20941 ttattgaaga aataactgct aacttttcca aaattttact gctaaaagat ggccaaagta
    21001 ttcaacaagg cgctgtagaa gacatattaa cttctgaaaa catgtcacga tttttccaga
    21061 aaaatgtagc agttcaaaga tggaataatc gattttctat ggcaatgtta gagtaaatat
    21121 tttgcaaata ataagtaata atgacaaaat ttaattaaga taaaatggac agtggagggc
    21181 aatatggata acgtaaaagc aatatttttg gacatggatg gaacaatttt acatgaaaat
    21241 aatcaagcat caacgtatac gaaagatgtc attaatcaat tgagagagaa aggatataaa
    21301 gtatttttgg caacaggacg ttcgcattct gaaatacatc aacttgtacc tcaagatttt
    21361 gcggttaatg gcatcattag ttcaaatgga acaattggag aagtagatgg agaaattatc
    21421 ttcaagcatg gtttatcatt ggctcaagtg caacaaatta ctaatttagc taagcgccaa
    21481 caaatttatt atgaggtatt tccttttgaa ggtaatagag tttctttaaa agaagatgaa
    21541 acatggatgc gagatatgat tcgtagtcaa gatcctatta atggcgtaag tcatagtgaa
    21601 tggtcttcaa gacaagatgc gcttgctggt aagatagatt gggtaactaa gtttcctgaa
    21661 ggtgaatatt caaaaattta tctattcagt tctaatttag aaaaaataac agcatttaga
    21721 gatgaattaa agcaaaatca tgtgcaacta cagattagtg tttcaaattc atcaagattt
    21781 aatgcggaaa caatggctta tcaaactgat aaaggtacag gcattaaaga aatgattgca
    21841 cattttggta ttcatcaaga agaaacgtta gttattggag atagcgacaa tgatagagca
    21901 atgtttgaat ttggtcatta tacagttgct atgaaaaatg cacgccctga aatccaagca
    21961 ttaacttcag atgtaacggc atacacgaat gaagaggatg gcgcagcaaa atatttagca
    22021 gagcattttt tagctgaata ataaaatagg tagttattta ttatttaatt tacaatagtt
    22081 gatgagtaat gtacaaagag cagtaaagtt attttctatt agaaaatgtc ttactgctct
    22141 tttgtatgct tataaatatt tgaat
//
LOCUS       DAKTLH010000028        21646 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.028, whole genome shotgun sequence.
ACCESSION   DAKTLH010000028 DAKTLH010000000
VERSION     DAKTLH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21646
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.028"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..232)
                     /locus_tag="O0394_000944"
     CDS             complement(<1..232)
                     /locus_tag="O0394_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0499034.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGT"
     gene            complement(238..1035)
                     /locus_tag="O0394_000945"
     CDS             complement(238..1035)
                     /locus_tag="O0394_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0499035.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAASITIITLTISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            complement(1028..2122)
                     /locus_tag="O0394_000946"
     CDS             complement(1028..2122)
                     /locus_tag="O0394_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0499036.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            complement(2135..2674)
                     /locus_tag="O0394_000947"
     CDS             complement(2135..2674)
                     /locus_tag="O0394_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0499037.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            complement(2818..3093)
                     /locus_tag="O0394_000948"
     CDS             complement(2818..3093)
                     /locus_tag="O0394_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCY0499038.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            complement(3261..4667)
                     /gene="lpdA"
                     /locus_tag="O0394_000949"
     CDS             complement(3261..4667)
                     /gene="lpdA"
                     /locus_tag="O0394_000949"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCY0499039.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNGIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            complement(4671..5963)
                     /locus_tag="O0394_000950"
     CDS             complement(4671..5963)
                     /locus_tag="O0394_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCY0499040.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATSEEVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            complement(6054..7031)
                     /locus_tag="O0394_000951"
     CDS             complement(6054..7031)
                     /locus_tag="O0394_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCY0499041.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            complement(7035..8147)
                     /gene="pdhA"
                     /locus_tag="O0394_000952"
     CDS             complement(7035..8147)
                     /gene="pdhA"
                     /locus_tag="O0394_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCY0499042.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            complement(8319..8945)
                     /locus_tag="O0394_000953"
     CDS             complement(8319..8945)
                     /locus_tag="O0394_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HCY0499043.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            9310..9861
                     /locus_tag="O0394_000954"
     CDS             9310..9861
                     /locus_tag="O0394_000954"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCY0499044.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
     gene            10191..10313
                     /locus_tag="O0394_000955"
     CDS             10191..10313
                     /locus_tag="O0394_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499045.1"
                     /translation="MNDMMNLVINQIVIKLHYRDDWNEFIDIYSNTILLGLTIN"
     gene            10342..10560
                     /locus_tag="O0394_000956"
     CDS             10342..10560
                     /locus_tag="O0394_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCY0499046.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            10560..12257
                     /locus_tag="O0394_000957"
     CDS             10560..12257
                     /locus_tag="O0394_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCY0499047.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
     gene            complement(12606..13268)
                     /locus_tag="O0394_000958"
     CDS             complement(12606..13268)
                     /locus_tag="O0394_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCY0499048.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
     gene            complement(13401..14420)
                     /locus_tag="O0394_000959"
     CDS             complement(13401..14420)
                     /locus_tag="O0394_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCY0499049.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFGISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            complement(14417..15778)
                     /locus_tag="O0394_000960"
     CDS             complement(14417..15778)
                     /locus_tag="O0394_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCY0499050.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            15976..16209
                     /gene="nrdH"
                     /locus_tag="O0394_000961"
     CDS             15976..16209
                     /gene="nrdH"
                     /locus_tag="O0394_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCY0499051.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHHIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            complement(16446..18164)
                     /gene="ptsP"
                     /locus_tag="O0394_000962"
     CDS             complement(16446..18164)
                     /gene="ptsP"
                     /locus_tag="O0394_000962"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCY0499052.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMGGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
     gene            complement(18167..18433)
                     /locus_tag="O0394_000963"
     CDS             complement(18167..18433)
                     /locus_tag="O0394_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCY0499053.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            complement(18587..19129)
                     /locus_tag="O0394_000964"
     CDS             complement(18587..19129)
                     /locus_tag="O0394_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HCY0499054.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRQR
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISNAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            complement(19183..20355)
                     /locus_tag="O0394_000965"
     CDS             complement(19183..20355)
                     /locus_tag="O0394_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCY0499055.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQQINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSAIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            complement(20780..>21646)
                     /locus_tag="O0394_000966"
     CDS             complement(20780..>21646)
                     /locus_tag="O0394_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499056.1"
                     /translation="YIVYKDELNQENVKNHFMWIIVSYGISYLITQIALYGRIDANEI
                     ESIDILSVNAFFIIMWLLGQMAIWNFLFLRRALPLTKQELGEEEPELSRTSKGNVTNQ
                     TKIHLKQLQDKTTEYARKTRRSVDLDKIRAKRDKFKKKVNDIIDIQEDDIPDWMRKPK
                     WVKPMYVELFCGVVIFLFTFLEFNNRNALFVSGDWKLSQTQYVIEWVTLLILLFIIIA
                     YIATTLTFHLKGKFYYLQLFMGSILFFKLLTEFINIMIHGLLLSVFITPTLLLMLLAI
                     IISYSLQLRERP"
BASE COUNT         7030 a         3973 c         3089 g         7554 t
ORIGIN      
        1 atgtaccaat aactgtagaa attgaggctg ctaaaagtgc tacagctatc gtattaaaaa
       61 tgaccgacat taaatgatca ttttgaaata atgattgata atgctctaat gtaaaatgtt
      121 caaagtgaat catattacca gccgaattga atgaatagaa cattaaaaag aatattggga
      181 tgtataaaat cgctaaaagt atcccgatat acagctttcc ataccatttc atatgattca
      241 ccctctccca ttagatgatt ttgtaatgat taaaatgaat gccataaata caattaagaa
      301 tatagctata gttgatccca taccataatt ttgaattgtt aaaaattgtt cctctattgc
      361 cgtacctata tttatgactt tattacctgc aattaatctt gtaatcataa ataatgaaag
      421 tgatggaata aaggttactt gaatcccagt cataacacct tcttttgtta acggcatgat
      481 tacttttcta aaagtataga aaggactggc acctaaatca cttgaggcct gcaataaatt
      541 attaggaatt gctttcatgc tattaaatat aggtaaaatc ataaatggta tataaatgta
      601 acttgccact actaaaaacg caccagttgt aaataacaaa ttgaatgatg gtaaattaaa
      661 taagtggaaa aattgattaa tcacgccatc atgacttaat aaacctataa aagcatatgt
      721 ctttaacaat aaatttatcc atgttggaat aatcattatc attaataaga tattttgaaa
      781 tttcgaacga gtaatataat aggcagctgg ataactgata gtcaaggtaa taattgttat
      841 tgaagcggca tataaaattg aatatgcaaa cattttcaaa tattttgtag taaaaatttg
      901 ttgataattc gtgaaactaa aatgtccatt gatatctaaa aatgaaaaat aaatgagtaa
      961 tacaactggt acaataataa atataaccat ccatagtaaa tacgggatta agagaaattt
     1021 attagtatta cgcattgtct acttcctcat agctttcaat acgtttatca aattcttctt
     1081 ctgtttctcc aggaaccata atatgaatgg cttcaggatc aaaataaaga ccaacttcac
     1141 tgcctacttc agctttttta gtcgtttgta ttacccattc ataaccttta ttgtctatac
     1201 aacatatttc atagtggacc cctctaaata acatagaatc aacagttgct ttaaataatc
     1261 cttcttcagc tttgattaat gatatatctt ctggtcgaat aacgacttct acttttttat
     1321 tttcaggaat acccatatcg acacattcga aatcttgccc ataaatattc acgacataat
     1381 ctctaaccat gcgcccttca acaatattag attctccaat aaaatcagct acaaatcgat
     1441 tcactggttc gtcatatata tctgttggtg tgccaaattg ttgaattttc ccatctttta
     1501 atacaaaaag aaagtcactt aatgctaacg cttcttcttg atcatgtgtt acaaatataa
     1561 atgtaatacc taatctagat tgcaattctc gtaattcata ttgcatttca gtacgcaatt
     1621 tcaaatctaa tgcggataaa gattcatcta acaataatat ttctggttca tttacgatag
     1681 cacgtgcaat tgcaacacgt tgcttttgtc cgccactcat ttcattaata tttctttttt
     1741 cataacctga aagttttact aattttaatg cctcagttac tttttgatca atttcggttt
     1801 ttgataattt ttttaatttc aaaccaaaag cgatattatc atagacgttt aagtgtggaa
     1861 ataatgcata atcttgaaag actgtattca ctttacgttt atttggtggt aaattaccaa
     1921 ttggtttgtt ttgataaatc acttcaccac tgtcaggata ttcaaaccct gcaattaatt
     1981 ttaaaattgt tgttttacca caacctgaag gacctaataa tgtataaaag tatcctgatt
     2041 caatatcaat atctatgtca tctaagatat taagatcatc atagctttta ctaactgatt
     2101 ttaatgataa taacggttcc aaataagatc ccccctataa atatgaagct gtcgctacaa
     2161 taagtattcg tgtttcattg tttgattcgt ttgacaaacg atgattatct cgtgctttaa
     2221 aatacaaaac gtcttcttct tgtgcttgat atatctcttt gcctaaatta agtgttatct
     2281 gacctgacat acaataaata aacgtatcag actctgatgg attaaaattt ttatatgatg
     2341 ctccaggctt taaagttaat attaatggct ccatatcata ttcatttgac tttgaaacta
     2401 accaatttaa tatataacct tcatcatact catcataaat aacttgttct tccttcttgt
     2461 ataatacttt ttcattttca ctgtctttaa aaaattcact tggcgtcgtt cctaacacct
     2521 ctataatatt taagaaagtt tccatacttg gtgaggcatg ttcactttct atttgtgaaa
     2581 tgtagccttt cgataagtct gtacgttcag caagttcttc ttgcgttaaa tttttaattc
     2641 ttctaagatt tttaatttta ttacctatgt tcatttattt ttccacctaa aaaagctata
     2701 ttaagtttac ttttatcaaa cattttgttt aacacttgat aaaggtatca aaatatagct
     2761 ttattagcaa tagttttaaa gaaaaattcc tattaaattg tgttaatttc gaacgaatta
     2821 ttttttagct gttactcttt gttcttcaga gtgagttttt acatcttgaa ctgctttgta
     2881 actattatag ccactacttt tttcgaaagt attaaaaatt tgtttttcct ctgctttagc
     2941 aggcacaatt tctttaaatc ttttatatgc acccataaag tcgcctgccg tcacgccgga
     3001 ttcataatac ttctctacat gattaaagaa atttatcact gaaatcatct cttcattact
     3061 ccagtctaaa tcaattggat actcatattc cattgttgaa ccctccattt caattctttt
     3121 gttagcatcg ctagtataac atgcatgtca catgttaacg atatctcaaa tcaatttcct
     3181 tacattactt tacctaaaac aaaaaagccg tagcatatgc tacagctttt aatgactgaa
     3241 tctttataga taatcagtta ttacattgta tggattgggt atccgatagc tttttctgct
     3301 gcttccatag tcatctcacc taatgttgga tgtgcatgga ttgttaatgc gatatcttca
     3361 gcattcatac cagcttcaat tgctaaacct aattcagaga taatatctga tgcaccagta
     3421 ccaactactt gagcaccgat taaagtatca tcttctttaa gtgtaataag tttaacaaat
     3481 ccgttagtat catctaatga taatgcacga ccatttgctg catatgggaa tttagaagct
     3541 ttaattgcta aaccttcttc tttagcttgc gcttctgaat aaccaactgt agctaattct
     3601 ggttcagtaa agcatactgc tggcatacca atgtaatcaa cttcagcagc ttgaccatca
     3661 attgcttcag cagcaacttt agcttcatag ctagctttgt gagcaagtgg taaacctgga
     3721 acgatatcac caattgcata gatattgctg atagacgtac ggctttgttt atcaacttct
     3781 aataatccac ggtcagcgaa tttaacacct aattcttcta ggcctaattc gtctgtgttt
     3841 ggacgacgac ctacagttac taatacataa tcagcttcga ttgttttctc ttcgccttta
     3901 gcttcataag taactttaac tccgttatct gtttcttcag ctgatttagc catagcttca
     3961 gtaacgattt caacaccttt ttctttcata ccttttttaa caggttgtgt catttgtttt
     4021 tcgaagccac ctaagatatc tttagcacct tcaaggatgg ttacttctga accaaagtta
     4081 gcaaatgctg tacctaattc tgatccaatg tatcctccac caactacaac taatttacct
     4141 ggtacttctt gtaagtttaa agcacctgtt gagtcgataa cacgtttacc gaatttgaaa
     4201 ttaggaattt caattggtct tgaacctgtt gcaatgattc catttttaaa gttgtatgtt
     4261 tgtgcgctct tttcgtccat aacacgtaag ctattgttat ctacgaaata tgcttcacct
     4321 ttaacgatgt taactttgtt acctttaagt aagccttcaa caccaccagt taatttatta
     4381 acaactgatg atttgaattc ttgaactttt tggaagttta aagaaacact ttcagcaata
     4441 acacctaagt tttcagaatg ttgtgcttca acaaaacggt gagaagcatg tagtaatgct
     4501 tttgaaggaa tacatcctac gtttaagcaa acaccaccaa gattaccttt ctcaacgatt
     4561 gttacttttt gtcctaattg agctgcacga attgctgcaa cgtatccacc aggacctgct
     4621 ccgattacta tagtatctgt ttcaattggg aaatctccaa ctaccatgtt ttacccctcc
     4681 attaataata attctggatt atttaataaa cgtttaatgt gattcattgc attttggcca
     4741 gttgcaccat caatttgtct gtggtcaaag cttaatgata atgctaatac tggtgctgca
     4801 acaatttctc catctttaac gataggtttt tgagcaatac ggccaattcc taagattgct
     4861 acttctgggt gattgataac tggagtgaac cattgtccac cagctgaacc gatattactg
     4921 attgtgcatg tagcaccttt catttcatcg gctgttaatt taccatcacg tgctttaaca
     4981 gctaattcat taatttcatc tgaaatttgg aaaatagact tacgatcagc atgtttaaca
     5041 acaggtacta ataatcctct atcagtgtct gctgcaatac cgatattcca gtaatgttta
     5101 tgaacgattt caccagcttc ttcattgaat gaagtgttaa gtgctgggta ttttttcaat
     5161 gcagaaacaa gtgctttaac aacataaggt aagaatgtta acttagtacc ttgttcagct
     5221 gcgatttctt taaatttctt acggtgatcc cataatgctt gaacatcaat ttcatccatt
     5281 aatgttacat gaggtgcagt atgcttagag ttaaccattg ctttcgcaat tgctctacgc
     5341 atagcaggga ttttttcagt tgtttctggg aagtcgcctt ctaatgttac tgctgcaggt
     5401 gctgcaggag tttcagcaac ttcttcactt gtagctgaag cagctgattc atttgaagct
     5461 gttggtgcac caccatttaa gtatgcatct acatcttctt ttgtaatacg accattttta
     5521 ccagatccag aaactgcttt aatgttaaca cctttttcac gtgcgtattt acgtactgaa
     5581 ggcattgctt taacagttct gttttcatct acttcttcag tttgagtagc tacaggtgct
     5641 tgctctgctg gcgcttcctc tttcgcaggt tcttctttag atgatgaatc atcatcatga
     5701 cctttaaatt gcatatcttc tgcatcaggt gcatcgattt taacaataac gtcaccaact
     5761 acagctactg taccttcttc taccataact tcttctacag taccagatac tggtgatggg
     5821 atttctacta ctgatttatc gttttgtacc tcagctaaaa catcgtcttc ttcaatagta
     5881 tctccagctt taacaaacca ttttacaatt tcaccttcgt ggataccttc cccgatatcg
     5941 ggtaatctaa attcaaatgc cacgtttttg ccctcctaag atttcgtaaa ttaaacattt
     6001 cattttaatc aatgagacta aaatacgcta acttcgttaa cttttaaaat gtattaaaat
     6061 tctaaagttt cttttgcttt ttcgatgatg tcatttttgt ttggtaacca aacattttca
     6121 gcttgagtga atggataaat tgtatctgct gctgcaactc ttccaatagg tgcttctaat
     6181 gaaaggattg cacgttcact taattcagct acaactgctg caccaacacc agcttgacgt
     6241 tgtgcttctt gaactacaac tgcacgacca gttttttcaa ctgaagctac aattgtgtca
     6301 acatcgattg gttgaacagt acgtaagtca attacttcaa cagaataacc atctttttca
     6361 agttcttctg cagctttcat tgattcttga accatcgcac cgtatgtgat gattgaaatg
     6421 tcattacctt cttttttcac attagcctta ccaatgtcaa ttgtatattc ttcttcaggt
     6481 acttcttcac ggaatgaacg atacaatttc atatgctcta agtatacgac tgggtcatta
     6541 cttctaatag aagaaattaa taaacctttc gcatcgtatg ggcctgaagg aataacaacc
     6601 tttagacctg gagattgagc taaaatacct tctaagttat ctgcgtgtaa ttctggtgtg
     6661 tgtacgccac caccaaatgg gctacgaatt gttacaggtg cagttttagt accgcctgaa
     6721 cggaaacgag tacgtgcaat ttgtccagca atcgcatcaa atacttcgaa tacgaaacct
     6781 aagaattgta cttccataac cggacggaat ccttcaactg caagacccat cgctaaacca
     6841 ccaatacctg attcagctaa aggtgtatcg aatactctat cttcaccaaa ttctttttgt
     6901 agtccttcag taacacggaa aacaccgccg ttaacaccaa cgtcttcacc aaaaattaaa
     6961 acatcttggt catttttaag ttcagtttta agcgcatcat taatcgcttg aaccattgtc
     7021 atttgtgcca tggcttactt cgactccttc tctttgtaaa tttcatattg ttctgctaag
     7081 ttttgaggca tatcttcata cataatttcc attagagaag taacagtttg tttttcagtg
     7141 ttatcagcct ctttaattgc tgctttaata tcagcttttg cacgttcaat aacttcattt
     7201 tctttgtctt cattccataa acctttgttt tcaaggaatt tacggaaacg tactaatggg
     7261 tcttttttct cccattcagc atcttcgtct gaagttctgt aacgagttgg atcgtcacca
     7321 gccattgtat gaggaccata acgatatgtc atagtttcaa ttaatgttgg accttcacct
     7381 gcaactgcgc ggtcacgtgc ttctttagtt gcttgatata cagctaacgc atccatacca
     7441 tcaacttgga taccaggaat acctacagca attgcttttt gagctaatgt ttcagcagca
     7501 gtttgcttgc ttcttggtgt tgaaattgca tagttattgt tttgaataac gaaaattgca
     7561 ggtgctttat aagctgctgc aaagttaata ccttcgtaga aatcaccttg tgaagaacca
     7621 ccgtcaccag tgtaagtgat tgcaactgca tttttaccac gttttttaag tgcaaatgca
     7681 acaccagcag cttgaatgta ttgtgcaccg ataataattt gtgggcttaa tgcattaacg
     7741 ccttcaggga attgatttcc tttgaagtga cctcttgaga ataagaaagc ttcagttaat
     7801 ggtaaaccat gccaaataat ttgaggaaca tctctgtatc ccggtaaaat gtaatcttct
     7861 ttttctaaag cgtattgtga cgctaattgt gatgcttctt gaccagcagt tggtgcatag
     7921 aaacctaaac gtccttgtct gtttaatgag atagaacgtt gatcaaggat acgagtccat
     7981 accattcttt ccattaattc cactaattgt tcatccgtaa gatcaggtac taagtcttca
     8041 tttacgacgt taccattctc atccaaaatt tgaaccattt caaatttcga ttgagtatca
     8101 tttaaaactt ttactgcatc gaattgggct tgtaacttag gagccattca attcaccata
     8161 cctttcccat aattgtatag aaattcattt tatccttact aattgtacca caaataaatg
     8221 ggactgttaa accatttgaa aaagttctgt tttaataaaa aaaagataca gataatagga
     8281 actgtattag ttgtttccta ccatctgtat tatattaatt atttaaattg atcaacgtct
     8341 tgcttttctt taccaacctt atttagtact tttgtatact tatctgatac ttcttttaac
     8401 tttttatagt tctgttctat tgcttttgat ttttcgttaa caccttgttg tgtcgcgtca
     8461 ttttgattta aatatttaaa taatgttttc tctgagttta cagccttttt atatgctttc
     8521 gcgtaatcac tgtgtaactt atatttttct tttaatacat catctaattg ttttacttct
     8581 ttacgtttaa catcattatc aatgttatca acgtgacttt ttgctttctt aaagtcttgt
     8641 tcagacttct taattgcgtc ttcttctttt tcaaattcct ttagacgatc atcggcattt
     8701 ttaattaaat cctttactgc tttaccgcga actgctgtat ctttactatt gacatcttca
     8761 gttaattttt tctttttctc atctaattca gcaattttct taccaacagt tttaattggt
     8821 tcttcatcat ctttaatttt atccacttgc tttaaatatg ccttaatttc ttttttatcc
     8881 gtagtacatc ctgcaagcaa gacactagat gctaatacta ctgcgattgt ttttccaaat
     8941 ttcattactt gtacctccac ttgctattta aattaaatga taaataaaat ctgcttggaa
     9001 atcaatttta aacaattaca ttacacatct tcattactaa gtatatctgt tgacagttac
     9061 gtcatttata atataatttt taaagtcaag atgataaata aatttctaaa gtatcattgc
     9121 ttgaaaccaa cactttaatc attttttaag caattagcat atctgaaaat ttacatatac
     9181 tatctaaaat aaacattggc agtagttggt taactttgta gatatttaca cataaaaatg
     9241 tatgagtatc ttaacaacct ttttactgtc attcaactaa aacaaattaa aataaggaag
     9301 gtgcaatata tgttaacaat gaaagacatc attagagatg gtcatccaac tttgcgtcaa
     9361 aaagcagctg agttagaatt accattaact aaagaagaaa aagaaacatt aatcgccatg
     9421 agagagtttt tagtaaatag tcaagatgag gaaatcgcga aacgatatgg tttacgttca
     9481 ggcgttggtt tggctgcacc tcaaattaat atttctaaac gtatgattgc tgttttaata
     9541 ccagatgatg gcagtggcaa atcttatgac tatatgcttg tgaacccaaa aattgtaagt
     9601 catagcgttc aagaagctta tttaccaact ggtgaaggtt gccttagtgt cgatgataat
     9661 gttgctggtc tagttcaccg tcataataga attacaatta aagccaaaga catcgaaggt
     9721 aatgatatac aattacgact aaaaggatat ccagcaattg ttttccaaca tgaaattgac
     9781 catttaaatg gtgtaatgtt ctatgatcac attgacaaag atcacccatt acaaccacat
     9841 acagatgcag tagaagttta aaacacattt ttctaattat caaagcttag gataaatatg
     9901 atgtcctaag cttttccttt tacaactttt tcgaataaac aacagttaaa tatattcacc
     9961 tttctaccaa actttttatc ccctcattta aattttaccg tttcatataa aatcctttaa
    10021 ttctttctta acattaattt ataatctcta catttatttt aataaataga actgcacatt
    10081 tattcgaaat acttagattt ctagtgagat aaactgcttt atttattatc attcatcatg
    10141 taaaataaga tttaactgaa attttagtgt tatttcacta attttttaaa atgaacgaca
    10201 tgatgaacct agttattaac caaatcgtta ttaagttaca ttatagagat gattggaatg
    10261 aatttatcga tatatactcc aatacgattt tactagggtt aacaataaat taaacaaaca
    10321 ttcttaggag gaatttttaa catggcagta tttaaagttt tttatcaaca taacagagac
    10381 gaggtaattg tgcgtgaaaa tacacaatca ctttatgttg aagctcaaac agaagaacaa
    10441 gtacgtcgtt acttgaaaga tcgtaatttt aatatcgaat ttatcactaa attagagggc
    10501 gcacatttag attacgaaaa agaaaactca gaacacttta atgtggagat tgctaaataa
    10561 tgaaacaatt acatccaaat gaagtaggtg tatatgcact tggaggtcta ggtgaaatcg
    10621 gtaaaaatac ttatgcagtt gagtataaag acgaaattgt cattatcgat gcaggtatca
    10681 aattccctga tgataactta ttagggattg attatgttat acctgactac acatatctag
    10741 ttcaaaacca agataaaatt gttggcctat ttataacaca tggtcacgaa gaccatatag
    10801 gcggtgtgcc cttcctatta aaacaactta atatacctat ttatggtggt cctttagcat
    10861 taggtttaat ccgtaataaa cttgaagaac atcatttatt acgtactgct aaactaaatg
    10921 aaatcaatga ggacagtgtg attaaatcta agcactttac gatttctttc tacttaacta
    10981 cacatagtat tcctgaaact tatggcgtca tcgtagatac acctgaagga aaagtagttc
    11041 ataccggtga ctttaaattt gattttacac ctgtaggcaa accagcaaac attgctaaaa
    11101 tggctcaatt aggcgaagaa ggcgttctat gtttactttc agactcaaca aattcacttg
    11161 tgcctgattt tactttaagc gaacgtgaag ttggtcaaaa cgtagataag atcttccgta
    11221 attgtaaagg tcgtattata tttgctacct tcgcttctaa tatttaccga gttcaacaag
    11281 cagttgaagc tgctatcaaa aataaccgta aaattgttac gttcggtcgt tcgatggaaa
    11341 acaatattaa aataggtatg gaacttggtt atattaaagc accacctgaa acatttattg
    11401 aacctaataa aattaatacc gtaccgaagc atgagttatt gatactatgt actggttcac
    11461 aaggtgaacc aatggcagca ttatctagaa ttgctaatgg tactcataag caaattaaaa
    11521 ttatacctga agataccgtt gtatttagtt catcacctat cccaggtaat acaaaaagta
    11581 ttaacagaac tattaattcc ttgtataaag ctggtgcaga tgttatccat agcaagattt
    11641 ctaacatcca tacttcaggg catggttctc aaggtgatca acaattaatg cttcgattaa
    11701 tcaagccgaa atatttctta cctattcatg gtgaataccg tatgttaaaa gcacatggtg
    11761 agactggtgt tgaatgcggc gttgaagaag ataatgtctt catctttgat attggagatg
    11821 tcttagcttt aacacacgat tcagcacgta aagctggtcg cattccatct ggtaatgtac
    11881 ttgttgatgg tagtggtatc ggtgatatcg gtaatgttgt aataagagac cgtaagctat
    11941 tatctgaaga aggtttagtt atcgttgttg ttagtattga ttttaataca aataaattac
    12001 tttctggtcc agacattatt tctcgaggat ttgtatatat gagggaatca ggtcaattaa
    12061 tttatgatgc acaacgcaaa atcaaaactg atgttattag taagttaaat caaaataaag
    12121 atattcaatg gcatcagatt aaatcttcta tcattgaaac attacaacct tatttatttg
    12181 aaaaaacagc tagaaaacca atgattttac cagtcattat gaaggtaaac gaacaaaaag
    12241 aatcaaacaa taaataatca aaaagctact aactttgaag tgaagtttta attaaactca
    12301 ccacccattg ttagtagctt tttctttata tatgatgagc ttgagacata aatcaatgtt
    12361 caatgctcta caaagttata ttggcagtag ttgactgaac gaaaatgcgc ttgtaacaag
    12421 cttttttcaa ttctagtcag gggccccaac atagagaatt tcgaaaagaa attctacagg
    12481 caatgcgagt tggggtgtgg gccccaacaa agagaaattg gattcccaat ttctacagac
    12541 aatgtaagtt ggggtgggac gacgaaataa attttgagaa aatatcattt ctgtcccact
    12601 cccgattatc tcgtcgcaat atttttttca aagcgattta aatcattatc atgtccaatc
    12661 atgattaaaa tatcacctat ttctaaatta atatttggat ttggtgaaat gatgaactct
    12721 ttgcctcgtt taattgcaat aatgttaatt ccatattgtg ctcttatatc taaatcaatg
    12781 atagactgcc ccgccatctt ttcagttgct ttcaattcta caatagaatg ctcgtctgcc
    12841 aactcaagat aatcaagtac acttgcactc gcaacattat gcgcaatacg tctacccata
    12901 tcacgctcag ggtgcacaac cgtatctgct ccaattttat ttaaaatctt tgcatgataa
    12961 tcattttgtg ctttagcagt tacttttttt acacctaact cttttaaaat taaagtcgtc
    13021 aacgtacttg attgaatatt ttcaccaatt gccacaatga catgatcaaa gttacggata
    13081 cctaaacttt tcataactgc ttcatctgta gtgtctgcaa caaccgcatg agtagcgata
    13141 tcactatatt cattcactct attttcatca tggtcgatgg ccattacatc catgtctaat
    13201 gcattcaact cacgaacgat actacctcca aaacgaccta gaccgatgac tacatattct
    13261 ttacccatac tcgccctcca ttaaatgatt ttcatcaatt cattgaaaat ataaatttaa
    13321 aattattata aatgagtacc ccaactaaat tatctaaatg cagtaatgca agtaaatgaa
    13381 agttggggta tcgtctcaac ttatgatttc tttccttcaa catattcttt gtcgaaaaca
    13441 aataatctta ataataatat taacgatgga agtaataaaa gtaaacctaa aataaagaca
    13501 atcactaatg tccagcccat ttctggatta acatatgcat ctgtaatttt tacaaacgga
    13561 tataaaaggt atggcaattt actaattcca tagccaaaga acgcgaacat catttgtaaa
    13621 ataacaaata caaaagccaa accatgtttt ttcttaaaga atgttaacaa tgaagctaat
    13681 gcaaagaata agaaacttat accaaacatc caccaatagt caaaaacagc tgaataaaaa
    13741 tgttcagaat tttgaatgcg taatgataga aatacgaata aacaaatgat aatcatcggc
    13801 ggccctaaaa atatgtgcca ttgtcttgtt aaattatatg ctggttcgtc atttgctttt
    13861 ttagcataat atgtcaaaaa tcctgatgaa atatataaaa ctgaaataat tgccaagaat
    13921 actacagacc aagcaaatgg gcttaataat aactgcaccc aatctagatc gataacattg
    13981 tttcgaacat taatatagcc accttctgta atagttaaag cagtagataa tgaagctgga
    14041 attaataatc cacttacccc atacatgatc atccatgcta attttgtatc ttgaccataa
    14101 ttttcaaatg cataaaaact gtttctcaac gatatcataa tcagtgctat tgaacctggt
    14161 attaacaata ccgtgcctaa atatttgatt gactctggaa agaaacctac gaatcctacg
    14221 aagaagaaaa caaagaatac attcgtaact tcccaaactg ggtttaaata acgtgaaatt
    14281 aagtgattaa ttttcttttc atcaccagtt aactttgaat gcaatgcgaa gaaacctgcc
    14341 ccaaaatcta tagaagcaat aatgatatag caaaataaaa acaaccataa cactgttata
    14401 cctataaatg cataaatcat ttttctattt ctcctccttg cttcttggct aaacgattta
    14461 catcttcata cgccggttta tttttaaaca tacgaattaa tacgtatgca catgtataca
    14521 ttaaaatgat gtacaatatg ccaaataaaa ttgtaacgaa ggttattccg cctgcttgtg
    14581 ttgctgcttc tgccacgcgc atataaccac gaacaatcca aggctgtcta cccatctctg
    14641 ttaagaacca tccaaattct atagctagca ttgaagctgg gcctgttaat aatattccat
    14701 aaagcatcca tttatgagta gaaaactttc taagcttttt aaacattaaa gttaagacat
    14761 aaacacctga aatgacaaaa cataaaattc ccatcgttac cattaaatca aagaaataat
    14821 ggacgatcat aggcggatgt aaactttttg gaaaatcatt taacccttgt actttagttt
    14881 tgacactatt atctgctaag aaactcaata gtccaggtaa ttcaatcgca cctttaactt
    14941 gctgagtctt ttcatctaac acaccaaata ataataattt ggcatgggaa gatgtatcga
    15001 aatgccattc ataagctgct aatttttcag gttggaattt atgcaaaaat tttgcagata
    15061 aatcccctgc caacatagaa agtaatgttg aaaagaatcc aactatcata gacattttca
    15121 aagctttctt atggtagaca gtatctttag gttgacgatt acgcaataat ttaaaagctg
    15181 ctattgatgc aataacaaat gccatcgtca taccggctgt agtaattacg tgaaatgatc
    15241 gaactataaa cgatgggtta aacatcgctt ctataggttg aacattgacc atctttccat
    15301 tcttcaactc aaaacctgca ggcgtattca taaatgaatt cactgaagta atgaagaatg
    15361 ctgagaaaga gccaccaata attactggta tactaattaa gaaatgtgtc catttatttt
    15421 taaaacgatc ccaagtatat aaatatatac ttaagaaaat agcttcaaag aagaacgcaa
    15481 atgtttccat aaataatgga agtgcaataa cgtgtccacc catttccata aatgtaggcc
    15541 aaatcaatga taattgaagt cctataattg tacctgtaac aactcccact gctacagtaa
    15601 ttgtataagc tttagcccat cttttggcca tagctatata ttgaagatca tttttgcgaa
    15661 tacctaaaaa ttctgcaatt gcgaacatta aaggcatacc aacaccaatc gttgcaaaaa
    15721 tgatatgaac tgctaaagtc atagctgtca aaaaccgact gatttcaact gtatccattt
    15781 aaaaacatca cctttttctt tttttgatga caacacaatg aacttaatta taattgctat
    15841 aatgtgtatt tttaaaacat tatgcgctat gaaattgtgt ataatttatg tcagttcaca
    15901 atgtgttcac aaatttgaat ttcaatgtat aattgtgtat attacattag aataaagcac
    15961 gaaggagcat gatacatgtc agaaataatc gtttatacgc agaatgattg tccaccttgt
    16021 acatttgtaa aaaattatct aaatgagcat cacattgatt ttgaagagag aaatatcaac
    16081 aatcaacaat atcgaaacga aatgatagat tttgatgctt tttcaactcc gtttattttg
    16141 ttgaatggca atccaatgta ccatgttgat cttgatgaaa tcaacaaagt attaaatatc
    16201 caagattaat tttcatctaa atcaacttca tcatttatag ctatcataaa ttaaatatcg
    16261 atttttaaat taactaacat taattaaaga actacatttt gttaatgaca catagttgtt
    16321 taattagtta gcaacaacat tccattgaaa ttaacataaa tcataaaaaa gaacccgaag
    16381 tattcataat tacttacaat gaatacttcg ggtttattta taattctgtc tttcgaaaga
    16441 attaattatt ttacgtagtt gttaactaat tcaataactt cttcttgcgt tgcacagtcg
    16501 actgcacggt tagcaagttc agtcatttca tttttactta aaccattaat ttgtcttctt
    16561 gctttcagaa tagacgttgc actcatagag aactcatcta aacctaaacc aagcaataat
    16621 ggaatagctg tttcatctcc agccatttca ccacacatac ctgtccattt accttcttta
    16681 tgtgacgctt caataacttg tttaactaaa cgtaagattg aagggttata tggttggtat
    16741 agatatgata cacgctctga catacggtca gcagctaatg tgtattgaat taaatcattt
    16801 gtaccgatac tgaagaaatc tacttcttta gcaaagacat cagctaatgc tgctgttgca
    16861 ggtatctcta ccatgattcc taattctata tcatccgaaa tgtcatgacc ttcattttta
    16921 aggttttctt tttcttctaa taatatagct ttagcttctc taaattcgtt aattgttgca
    16981 accattggga acatgatatt taacttacca taaactgatg cacgtaataa tgcacgtagc
    17041 tgtggtctga aaatatcttg ttgcgcaagg cataaacgaa tcgcacggta acctaagaat
    17101 ggattcattt cttcaggcaa gtttaagtat gataattctt tatctccacc tatatctaaa
    17161 gtacgtacaa caacacgttt accgcccatt gcttctaata cttctttata agcttcaaat
    17221 tgttcttctt ctgtaggcat ttggtcacga cccatatata aaaactcagt tctatataag
    17281 ccgatacctt gtgcaccatt ttcaataaca cctggcaaat cattaggtgt accaatattt
    17341 gcagcaagct ctgcgtgaac accatcaact gtaacagtat cagcatcacg tagtttttgt
    17401 aattctttct tgtcagcaaa ataacgctca cgtttatctt gataagcgat taactcatct
    17461 tcagttggat taacgattac atcaccattt aatccatcta cgataatcat gtcgccttgt
    17521 ttaacttctt gagtaattga ttttgtacca acaattgctg gaatttctaa agaacgactc
    17581 ataattgcag agtgacttgt tcttccgcca atgtttgtag caaaaccttg tacgaattct
    17641 ttatttaatt gagcagtatc agatggcgtt aagtcattcc ctacaataac aacgctttca
    17701 tcaatcatac tcggattcgg taattctaca cctaaaatat gtgataacac acgtttagaa
    17761 acgtcgcgaa tatcagccgc acgttctttc atgtattcgt tatccataga ttcaaaaatt
    17821 gtaacaaatt gtgttgttac atccgttaat gctgtagcag cattagcgtt ttcattttta
    17881 atcttatctt ggattggttg aattaattca gggtcatcta aaactaataa atgtgcatca
    17941 aagatagcag ctttatcagc acctagttga acctctgcat tatttctaat tttagttaac
    18001 tcaactttag aagcttcgat agcgctattg aactttgcaa cttctccttc aacatcagtg
    18061 actttttcat ttttgtcgaa tgttaagtct ggctcaacta ataaataagc tttagcaatt
    18121 gcgacaccat cagatgcggc aataccttta attaatttag acatgattat ttagtcaatc
    18181 cttcttttga taagacgtca ctgattgctt gaatggcgtc agattcatca ctaccgtcag
    18241 cataaattgt aatttcagca tctttaccaa caccaaggct cataacaccc atgattgatt
    18301 ttaagtttac tttcttaccg ttatattcta attgaatatc agaatcgaat tttgaagctg
    18361 tttgtactaa cattgttgct ggtctagcgt gaataccagt ctcgtcgatg attacatatg
    18421 aattttgttc cataattaca tttctccttc gtatagttag aatcttcaat attattacaa
    18481 tactaactta gttttataca ttaactttac caaaaccatg ctttaaattc aattcgttta
    18541 aaaccggttt cagtaaaatt tgcaaattgc aaaactatta tagcacctac atgacatttt
    18601 taataacatt ttcgcatttt tgaatgtgat cttttccaat tttattcata aaatagtaac
    18661 taatagatgc agacacagct tgtcctacaa caggaaacca ttttgtttgt tttgcagcag
    18721 tacgtttagc tacatctcta attacaattt ttaaaaatgc atttgaaatt cttttaccaa
    18781 taaattgact accttgaatt gctgctgcag acataattct ttctttcaca tcatccccaa
    18841 ggctattaac ttgcttatga tctaaaccat aaattttatt aacatcttcg ataatatctt
    18901 tcattaattt taaatcaaca ccaaaatcta aacccggaat tggaacaacg ctcattgttg
    18961 atgacattaa agactttctc tttacaattg cttcagcacg ttgtcgtctt tctctcaatt
    19021 cttgaatcgt cgttggcatt gcaccttttc cgtcaacatt ttctatttta aagactgaat
    19081 taccgatttt atttgttaaa tttgatgtta aattgttttt gaatcccata atattatcac
    19141 ccttcttttt ctcaataagt tactataccc ctttttatca aattaatgtc taatatttac
    19201 aaaaacagct tttaaatatt tagatggctt ataatgtgga tgcgttttaa aatcttttgg
    19261 taatcccata acttcagtta attcataatc aacgccactc tcttcaagcg tctttttaat
    19321 agtattttta aattgcttta atggatatac acttgcgttt gtacataaca ataatgttcc
    19381 ttcagatgat aagatattta aggcgccatt aattaatttg tcataatctt tttgcactga
    19441 aaatgtacgt tttttgttac gcgcaaagct aggtggatca atcacgatcg tgtcataact
    19501 atgtccatgt cgtgcagcat atttatagaa atcaaaagtg tccatgacat aaatatattg
    19561 ggatttagga tcaatagcat ttaatccaaa attttcttca gttaaactac gagaacgatt
    19621 agccaaatct acacttgttg ttgaagatgc ctcacttgct gctattgcag aaaaagcacc
    19681 tgtataacta aataagttta aaacatggcg ttctttggca tattgatccc ttaatttctt
    19741 gcgcacttct ttttgatcta aaaagatacc tgtcatcaaa ccatcttcaa ggtctacatt
    19801 ataaaatgtg aagttttctt cgataacaat tggaaactca ggtgcatctc cttcaacaaa
    19861 accaccacta tattcgctgt ctttaaatct tactttttcg taaatagatt tataatcaaa
    19921 tacttttcta accgcttcaa gaatggcata tttaaattta taaatacctt ttgagtacca
    19981 ttgaatcaac aaatgaccat cgtaattgtc gattgttaat cccccaacac catcaccttc
    20041 agcattaaat aatctaaaag catttgttcc atctatatta aaataataat cacgttctgc
    20101 taatgcagta ttaaacaatt ttacaaagaa agctgtgttg atttgttgag ctttatcgta
    20161 tgttagaacc caacctaatc ctttatgttg gcgaccaaca taagccgttg cgacatattg
    20221 tgatttatca gtcacaattt gaaaaatatc tccttctttt aaatgatctt gtgaatagat
    20281 atcctcttct tcaattaaag gatatccatt aaaatatttt gtttctttgc ctttgttcag
    20341 agttgctatt ttcatctgac aacttccttt ctgtgtttcg ttgtagtttt atatagatta
    20401 aattgtttgc caaatttcaa tatcatctac ttctaatctt actgttgatt ttaacaaatt
    20461 agtaattcga atgatatatg ttaaatttct acaaaggaaa tactatatat cgtttcgcaa
    20521 attccataag tcgcttcaac tatttactag ttttcttgat tttacaaatg gataactttt
    20581 caactatttt atttgcacta aaactttgta tacaattttc aatacttaat tcacatcacc
    20641 tgaatcaatc aaatctagtt acaaaaaaag tactatccat aaacttcatt gacctgtatc
    20701 taagtaagtt agagatatat ctaacttata gcgtacacat caaaatgttt aatagatagt
    20761 acttttataa tgcttttaat tatggtcgct ctcgtaattg taacgaataa gaaatgatga
    20821 ttgccaataa cattaatagt aatgttggcg taatgaacac tgaaagtaat agtccatgaa
    20881 tcattatatt tataaattcc gttaacaatt taaagaataa aatgctcccc ataaataatt
    20941 gtaaataata aaacttacct ttcaagtgga aagttaacgt tgtagcgata tatgcgataa
    21001 taatgaataa cagaattaat aatgtaaccc attcaataac atattgtgtc tgtgataatt
    21061 tccaatcacc agatacaaat aatgcattac gattattaaa ttctaaaaat gtgaataaaa
    21121 agatgacgac accacaaaat agttcgacat acattggttt aacccatttc ggttttctca
    21181 tccaatcagg aatatcgtct tcttgaatat cgataatatc attaactttc tttttgaatt
    21241 tatctctttt agctctaatt ttatctaaat cgacacttct tcttgtctta cgtgcatatt
    21301 ctgtagtctt atcttggagt tgtttcaagt gaattttagt ttgattcgtg acattccctt
    21361 tacttgttct tgataattct ggctcctctt cacctaattc ttgctttgtt aaaggtaaag
    21421 ctcggcgcaa gaacaagaaa ttccaaatag ccatttgacc aagtaaccac ataattataa
    21481 agaaagcatt gacacttaag atatcaattg actctatttc attagcatca attctgccat
    21541 acaatgcaat ttgtgtaatt aagtaactta taccataact cacaattatc cacatgaaat
    21601 gattttttac attttcctga ttcaattcat ctttatagac gatata
//
LOCUS       DAKTLH010000029        21554 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.029, whole genome shotgun sequence.
ACCESSION   DAKTLH010000029 DAKTLH010000000
VERSION     DAKTLH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21554
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.029"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            119..1066
                     /locus_tag="O0394_000967"
     CDS             119..1066
                     /locus_tag="O0394_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCY0499057.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
     gene            1067..1534
                     /gene="ybeY"
                     /locus_tag="O0394_000968"
     CDS             1067..1534
                     /gene="ybeY"
                     /locus_tag="O0394_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCY0499058.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            1537..1881
                     /locus_tag="O0394_000969"
     CDS             1537..1881
                     /locus_tag="O0394_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCY0499059.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            1892..2296
                     /gene="cdd"
                     /locus_tag="O0394_000970"
     CDS             1892..2296
                     /gene="cdd"
                     /locus_tag="O0394_000970"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCY0499060.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            2297..3196
                     /gene="era"
                     /locus_tag="O0394_000971"
     CDS             2297..3196
                     /gene="era"
                     /locus_tag="O0394_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCY0499061.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            3224..3970
                     /gene="recO"
                     /locus_tag="O0394_000972"
     CDS             3224..3970
                     /gene="recO"
                     /locus_tag="O0394_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCY0499062.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYAALAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(4120..5511)
                     /locus_tag="O0394_000973"
     CDS             complement(4120..5511)
                     /locus_tag="O0394_000973"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HCY0499063.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            5846..6469
                     /locus_tag="O0394_000974"
     CDS             5846..6469
                     /locus_tag="O0394_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0499064.1"
                     /translation="MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHKVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            6480..7298
                     /locus_tag="O0394_000975"
     CDS             6480..7298
                     /locus_tag="O0394_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCY0499065.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDINPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            7359..9158
                     /locus_tag="O0394_000976"
     CDS             7359..9158
                     /locus_tag="O0394_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCY0499066.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQNLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPE
                     PIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDF
                     YAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEKGQETIE
                     SLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            9382..10488
                     /gene="rpoD"
                     /locus_tag="O0394_000977"
     CDS             9382..10488
                     /gene="rpoD"
                     /locus_tag="O0394_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCY0499067.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            10619..11296
                     /locus_tag="O0394_000978"
     CDS             10619..11296
                     /locus_tag="O0394_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCY0499068.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     ELIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            11299..12399
                     /locus_tag="O0394_000979"
     CDS             11299..12399
                     /locus_tag="O0394_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCY0499069.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDAYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKALLMNWFNTEKINLDVEASTINTDPFQYI"
     gene            12513..13859
                     /locus_tag="O0394_000980"
     CDS             12513..13859
                     /locus_tag="O0394_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCY0499070.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFYLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            13869..14759
                     /locus_tag="O0394_000981"
     CDS             13869..14759
                     /locus_tag="O0394_000981"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCY0499071.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            14885..15670
                     /locus_tag="O0394_000982"
     CDS             14885..15670
                     /locus_tag="O0394_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCY0499072.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            15712..16575
                     /locus_tag="O0394_000983"
     CDS             15712..16575
                     /locus_tag="O0394_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCY0499073.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            16562..16972
                     /locus_tag="O0394_000984"
     CDS             16562..16972
                     /locus_tag="O0394_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCY0499074.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
     gene            17249..17848
                     /locus_tag="O0394_000985"
     CDS             17249..17848
                     /locus_tag="O0394_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCY0499075.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            17969..20044
                     /locus_tag="O0394_000986"
     CDS             17969..20044
                     /locus_tag="O0394_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCY0499076.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIIFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDK
                     QLLSKIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVTINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETRGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGP
                     LKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     NDKNKDKDK"
     gene            20157..20306
                     /gene="rpmG"
                     /locus_tag="O0394_000987"
     CDS             20157..20306
                     /gene="rpmG"
                     /locus_tag="O0394_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCY0499077.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            20515..21054
                     /locus_tag="O0394_000988"
     CDS             20515..21054
                     /locus_tag="O0394_000988"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCY0499078.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQAA"
     gene            21066..>21554
                     /locus_tag="O0394_000989"
     CDS             21066..>21554
                     /locus_tag="O0394_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCY0499079.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTI"
BASE COUNT         7989 a         2910 c         3972 g         6683 t
ORIGIN      
        1 aagaaaaatg agtaagtcaa tgcaaagatg tttaaatcaa tcaattgcat gatataatta
       61 agtagatatt aaagcatcat agaatgaata taaatgatat atgaaaagga gcgcgtgtat
      121 gcctggaatt atacaaatag acgatatgaa ccaatctcaa gctttaattg gaaataatga
      181 tgaacattta aaagcaattg aagagagttt cgatgttgtc atccatgcaa gaggacaaga
      241 agttgccgtt aaaggtacaa aaatagaaaa cgtagaaaaa gcggaatcag tattaatcaa
      301 tttgctgaag gttattgatt taggtaataa tattacaatt aaagatgttg aagcagctat
      361 taaaatggcg cataataaca caattcaaca tctgttagat ttatatgatg aagagataac
      421 taaagatgca tttggtaaga cgattcgtgc gaaaacgatg gggcaacgta tatatgttaa
      481 tgccatgaaa aataatgatt tagtatttgg tataggtcct gctggtacag gtaagacatt
      541 cttagctgta gtttatgcag caaagcaact ccgtaaaggt gctgttaaac gtattgtatt
      601 aacaagacct gctgttgaag caggagagtc acttggattt ttaccaggag atttgaaaga
      661 aaaggtagat ccatatttaa gacctttata tgatggtcta tatactgttc ttgggcgtga
      721 acaaacagag cgatttattg aaagaggcat tatcgaaata gcgccacttg catatatgcg
      781 cggacgaaca ttagaagatg catttgtaat tcttgatgag gcgcagaata cgacacatgc
      841 gcaaatgaaa atgtttttaa caagactagg ttttggctca aaaatggtag ttactggtga
      901 ccaaactcaa atcgatttac ctaaaggtgt taaaagtgga cttaaggaag cggtcagtag
      961 gttacacaac gttaaaggta taagtatatt gaaattagat cagagcgatg tagtcagaca
     1021 tccattggta agtaagatca ttgaacatta tgaaggagag aattaaatgt ttacgataga
     1081 ttttagcgat cacacaggct tagttaaaga tgcttggtat aaacaaattg aagatttatt
     1141 agaatttgct aaaaaagaag agcatataga agacgatgct gagctttctg ttacatttgt
     1201 agataaacaa gaaatacaag aaattaatcg aacatataga gataaagata aagttacaga
     1261 tgtaatctca tttgctttag aagaagatga gccagagatt gattttagtg gtcttgatat
     1321 accacgtgtt ttaggggata taattatctg tacggatgta gcgcaagaac aagcaaacaa
     1381 ttacggacat tcttttgaac gagaattagg atttttagca ttacatggat ttttgcatct
     1441 attaggttat gatcatatga ctgaagcgga tgaaaaggaa atgtttggtc gacaagatac
     1501 aatattaaac gcatatggat taacacgaga ctaattatga aaaggtttaa atatgcactt
     1561 gatgggctga aaatcttaat tcaaaaagac tataaatttc ttttacatgt gtttgcaatg
     1621 attgttgcta ttgtctttgg tctcgtacta aatattaatc ggattgagtg gatatttata
     1681 ctcattgcta ttgcattagt tctcactgtt gaagctttaa acactgctat tgaatatgtt
     1741 gtcgatttag tgaccgttga atatcatgat ttagctaaat acgctaaaga tattgcggct
     1801 tttagtgtac ttatagtttc aatattagca tttattatag gtttaatagt atttttacca
     1861 cattttatag cgttatttta gggaggcata tatgagttat caacctcatt attttcaaga
     1921 agttagaaaa gcacaacaag aatcatattc gccatacagt caatttaaag taggggctta
     1981 tttaaaaacg aaagacggta gaacttttta tggtaccaat gtagaaaatg cttcttatcc
     2041 attatcgata tgtgctgaac gagctagttt ggtatcggca atttctcaag gatacagacc
     2101 aggtgatttt gaatcaataa ctgtaaccgt agatgcagat aaaccgtcat caccttgtgg
     2161 tgcatgtcgt caagttttga aggaattatg tgatgatgat atgcctgtgt atatgacaaa
     2221 tcataaagga gatatggtta tgatgacagt cgcagagtta ctaccatttg gattttcagg
     2281 aaaggattta gaataaatga cagaacataa atcaggattt gtttcaatta taggtagacc
     2341 aaatgtagga aagtcaacat ttgttaatag agtgatcggc cataaaatag caatcatgtc
     2401 cgataaagct caaacaacta gaaataaaat tcaaggtgtt atgacaagag atgacgcgca
     2461 aattatattc attgatacgc caggtattca taaacctaaa cacaaattag gtgactatat
     2521 gatgaaagtc gctaaaaata cattatctga gatagatgca atcatgttta tggttaatgc
     2581 caatgaggaa attggacgag gcgatgaata tattatagaa atgttgaaaa atgttaagac
     2641 accagtattt ttagtattaa ataaaataga tttagtgcat ccagatgaat taatgccaaa
     2701 gattgaagaa tatcaaagtt atatggactt tacagagatt gtacctattt cagcattaga
     2761 agggctaaat gtcgatcatt ttattgatgt tttaaagacg tatttacccg aaggacctaa
     2821 atattatcca gatgatcaaa tttcagacca tcctgaacaa tttgtagtgg gtgaaatcat
     2881 tcgtgaaaaa atccttcatc ttacaagtga agaaatccct catgcgattg gtgttaatgt
     2941 ggaccgtatg gttaaagaaa gcgaagatcg tgttcatatc gaagcaacta tatatgttga
     3001 aagagattcg caaaaaggaa ttgtcattgg aaaaggcggt aaaaagttaa aagaagtagg
     3061 aaaacgtgcg agacgtgata tagaaatgct tctaggctct aaagtttact tagaattatg
     3121 ggtcaaagtt caaagagact ggcgaaacaa agttaacttt attcgccaaa ttggttatgt
     3181 tgaagaccaa gattaatctt aaaagtggtg aagataattg ttaatgcgcc aaaaagggat
     3241 tatcatcaaa gcagttgatt atggtgaatc tgataaaatt atcacgattt taaatgagca
     3301 tggtgcaaaa gtaccactta tggcaaggcg tgctaaaaaa gttaagacgg gtttacaagc
     3361 gcaaacgcaa ttgtttgttt atggtttgtt tatttacaat cagtggcgag gtatgggaac
     3421 gttaaattct gtagatgtta ttagtcaaca ttataaatta caaatggacc tttacgtaag
     3481 cagttatgcc gctctggcag ctgaaactat tgagcgctca atggatgaag gtgacattgc
     3541 accatataac tatcaattat tacaatttgt tcttgaaaaa atagaatcag gtacatctgc
     3601 acagttaatg tcagtcgtag ttatgttaaa gtgcatgaaa cgatttggtt ttactgcatc
     3661 atttaatcgc tgtgctgtga gtggtaatga cacacaagca gatttaatag gttatagttt
     3721 taagtttgac ggtgcgattt caaggcaaga ggcttctaaa gatgtacatg cagttatatt
     3781 atcgaataaa acactatatt tattagatgt attacaaaaa ttaccgatag ataaaatgaa
     3841 ttcattgaat atccatcaag aaattattga tgaaatgtca gatatcattt taatgttata
     3901 tcgtgaatat gcaggtatgt tttttaaaag tcagaaacta atcaaccaat taaaaagatt
     3961 ggaacaataa cataaaataa taaaaggtat tcaagtagcc acatagatgt gtttatttga
     4021 ataccttttt gaatagaaaa gagatactgg caattttact aaccagtatc tctttttata
     4081 aattatatag ccaccacata tggtggaaag tctttttaat tagaattttg ttttttcagt
     4141 taagaaagct tctaactctg agattggcat acgaacttgt tccattgaat ctctgtcacg
     4201 tactgtaact tgattatctt ctaatgaatc aaagtcgaat gttacacaat aaggtgtacc
     4261 gatttcatct tgacgacggt atcttttacc gatagattgt gattcatcga aatcgattga
     4321 gaatttagaa cttaattgct caaaaatctt aatcgcttcg ccagataatt tcttacttaa
     4381 aggtaaaatc gctgctttat atggtgctaa tgcaggatgg aagtgtaaaa ctgtacgtgc
     4441 atctttacta ccttcaacgc cttcttcatc atatgcatca cataagaaag ctaatgttac
     4501 acgatctgca ccaagtgatg gctcgataca atatggaata tatttttcgt tcgtttctgg
     4561 atcatggtat ctgaaatctt caccagagtg ttcagcatgt ttacgtaagt cgaagtctgt
     4621 acgacttgcg ataccccata actcacccca accaaatggg aatttatatt caatatcagt
     4681 tgttgcattt gagtaatgag ataattcatc ttcatcatga tcacgtaaac gcatattttc
     4741 actgctcata tttaagcttg ttaaccagtc acttgcaaaa gttttccaat aattttgcca
     4801 ttcgatttct tctccaggtt tacagaagaa ttcaagttcc atttgttcaa attctcttgt
     4861 tctgaaaatg aagttacctg gagtgatttc attacggaat gatttaccaa tttgaccgat
     4921 accaaatggt aattttttac gcattgaacg ttgcacgttt ttatagttta caaaaatacc
     4981 ttgtgctgtt tcaggacgta agaaaatttc atttgtagaa tcttcagtta caccttggaa
     5041 tgttttaaac attaaattga attggcgaat ttcagtccag ttagcagttt tacttacagg
     5101 acaaacaata ccttcatcgt caataatttt tttcatttgt tcaaaactta aaccatcggc
     5161 aatgaagttt tcatcacctt taacatcttg catataatct tcaattaatt tatcagcgcg
     5221 atatcgaatt ttactatctt tattatcaat cattgggtcg ttgaagttgt ttaagtgtcc
     5281 tgaagcttcc catacttttg gattcattaa gattgcagca tcgataccaa cgttaaacgg
     5341 tgattgtgta atgaattttt gccaccaagc ttttttaaca ttattcttta attcaacacc
     5401 taaaggacca taatcccatg tgtttgataa accaccgtaa atatcactac cagggaacac
     5461 aaaacctctg tgttttgcta atgaaacaat tgtatccata tcttttgcca taattacctc
     5521 tcctcataca tgaaaacgcc ccatggaaaa taacagcata gtaaatatgc tttacttcca
     5581 tgggacgagt taatatttta aattgtatat aatacaaaat aagttacgta tttaacccgc
     5641 ggttccaccc aaattagtgt agtcactcgc ttttatttta aaatgattcg ttgcgccaat
     5701 cttattgtta agcttacact atccttaact cgctgtacgt ttattctaat tgatagatta
     5761 tttaaaggca agtaaaatta atgtgtttgt gacatgaaag aaatatcggt ataataatag
     5821 gtgaataaaa aaggggtgaa gacctataga actcagtcaa agacaagaac gaatcatcga
     5881 aattgttaaa actaaaggac ccattactgg tgaacaaata gcagataagt tgaatttaac
     5941 aagagcaacg cttagaccag atttagcgat attaacaatg tcaggttttt tagaagcgcg
     6001 tccccgagtt ggatattatt attcaggtaa atcaaaaggc aaatttttta atgaaaaact
     6061 tcgacaattt gaggttaagg actatatgtc tcaacctgtt gtgctaagag agaatacaac
     6121 agtttatgac gctatttgta caattttttt agaagatgta agtacattat ttattattaa
     6181 tgaagataac gattttgttg gtgtgtgttc aagaaaagat ttattaagag cttcaatgat
     6241 tggagcagat attcataaag tacctatcag tgtaaatatg acacgtatgc ctaatgtcac
     6301 ttatttagag gaaagcgaat tagtcatata cgcagcagat agaatgattg aaaaagaaat
     6361 tgattcgatt ccaattgtaa gaaaaaaaga taatcaaaag tatgaagtaa ttggaagaat
     6421 ttccaaaaca acaatagcta agttattagt agcattatat aaagaatagg tgagaagtaa
     6481 tggaaaaaat taaaattatc gtagcttcag attctatagg tgaaacggca gagttagttg
     6541 ctagggcagg tatttcacaa ttcaatccta agcaatgtaa aaatgaatta ttaagatatc
     6601 catatattga atcttttgaa gatgttgatg aagtgattca agttgcaaaa gatacaaatg
     6661 ctatcattgt ttatacactt attaaacctg aaatgaagca atatatgagt gagaaagtag
     6721 cagaattcca attgaagtct gtcgatatca tggggccatt aatggattta ttatctgctt
     6781 cggttgaaga aaaaccttat aatgagccag gtatcgttca tagattagat gatgcatatt
     6841 tcaagaaaat tgatgcgata gagtttgcag ttaaatatga tgatggtaaa gatcctaaag
     6901 gattacctaa agctgatatt gttttacttg gtatttcgag aacttcaaag acaccattat
     6961 ctcagtattt agcgcataag agttacaaag ttatgaatgt accgattgta ccagaagtga
     7021 caccgccaga tggcttatat gatattaatc caaagaaatg tatcgcactt aaaataagtg
     7081 aagaaaaatt aaatcgcatt agaaaagagc gactaaaaca attaggacta ggtgacacag
     7141 ctcgatatgc aacagaagca cgaattcaag aagaattgaa ttactttgaa gaaatcgtaa
     7201 gtgaaattgg atgtcctgtc attgatgttt ctcaaaaagc aatcgaagaa acagcaaacg
     7261 atataatcca ttatattgaa caaaataaat cgaaatgatt tcatttttgt cgaaaattag
     7321 gtataatagt ataactaatg cttaataggt gatttaattt gcgaatagat caatcgatca
     7381 ttaatgaaat aaaagataaa accgacattt tagacttggt aagtgaatat gtaaaattag
     7441 aaaagagagg acgcaattat ataggtttgt gtccttttca tgatgaaaag acaccttcat
     7501 ttacagtttc tgaagataaa caaatttgtc attgttttgg ttgtaaaaaa ggtggcaatg
     7561 tttttcaatt tactcaagaa attaaagaca tatcatttgt tgaagcggtt aaagaattag
     7621 gtgatagagt taatgttgct gtagatattg aggcaacaca atctaactca aatgttcaaa
     7681 ttgcttctga tgatttacaa atgattgaaa tgcatgagtt aatacaagaa ttttattatt
     7741 acgctttaac aaagacagtc gaaggcgaac aagcattaac gtacttacaa gaacgtggtt
     7801 ttacagatgc gcttattaaa gagcgaggca ttggctttgc acccgatagc tcacattttt
     7861 gtcatgattt tcttcaaaaa aagggttacg atattgaatt agcatatgaa gccggattat
     7921 tatcacgtaa cgaagaaaat ttcagttatt acgatagatt tcgaaatcgt attatgtttc
     7981 ctttgaaaaa tgcgcaagga agaattgttg gatattcagg tcgaacatat accggtcaag
     8041 aaccaaaata cttaaatagt cctgaaacac ctatctttca aaaaagaaag ttgttataca
     8101 acttagataa agcgcgtaaa tcaattagaa aattagatga aatcgtatta ctagaaggtt
     8161 ttatggatgt tataaaatct gatactgctg gcttgaaaaa cgttgttgca acaatgggta
     8221 cacagttgtc agatgaacat attactttta tacgaaagtt aacatcaaat ataacattaa
     8281 tgtttgatgg ggattttgcg ggtagtgaag caacacttaa aacaggtcaa aatttgttac
     8341 agcaagggct aaatgtattt gttatacaat tgccatcagg catggatccg gatgaataca
     8401 ttggtaagta tggcaacgat gcatttactg cttttgtaaa aaatgacaaa aagtcatttg
     8461 cacattataa agtgagtata ttaaaagatg aaattgcaca taatgacctt tcatatgaac
     8521 gttatttgaa agaactaagt catgatattt cgcttatgaa atcatcgatt ttgcaacaaa
     8581 aggctttaaa tgatgttgca ccatttttca atgttagtcc tgagcaatta gctaacgaaa
     8641 tacaattcaa tcaagcacca gccaattatt atccagaaga tgagtatggc ggttacattg
     8701 aacctgagcc aattggtatg gcacaatttg acaatttgag ccgtcaagaa aaagcggagc
     8761 gagcattttt aaaacattta atgagagata aagatacatt tttaaattat tatgaaagtg
     8821 ttgataagga taacttcaca aatcagcatt ttaaatatgt attcgaagtc ttacatgatt
     8881 tttatgcgga aaatgatcaa tataatatca gtgatgctgt gcagtatgtt aattcaaatg
     8941 agttgagaga aacactaatt agcttagaac aatataattt gaatgacgaa ccatatgaaa
     9001 atgaaattga tgattatgtc aatgttatta atgaaaaagg acaagaaaca attgagtcat
     9061 tgaatcataa attaagggaa gctacaagga ttggcgatgt agaattacaa aaatactatt
     9121 tacagcaaat tgttgctaag aataaagaac gcatgtagca tgtgatttta aagaataata
     9181 cgaataatga ttatgtcaaa atgtataagg gtaaatgata gttaccgcat ttaaacaaca
     9241 ctattgaaaa ataaatattg ggattagttc caatttgtaa aataaaatta aaaatatgga
     9301 tgaattaatt aagaatttag tttaaaatag caatattgaa taaatttcga atgttcatat
     9361 ttaaaatcgg gaggccgttt catgtctgat aacacagtta aaattaaaaa acaaacaatt
     9421 gatccgacat taacattaga agatgttaag aagcaattaa ttgaaaaagg taaaaaagag
     9481 ggtcatttaa gtcatgaaga aattgctgaa aaacttcaga attttgatat cgactctgat
     9541 caaatggatg atttctttga tcaattaaat gataatgata tttcactagt taatgaaaaa
     9601 gatagttcag atactgacga gaaactgaat ccaagtgatc ttagtgcccc tccaggtgtt
     9661 aaaataaatg acccagttcg tatgtacctt aaagaaattg ggcgtgttaa cttattaagt
     9721 gcacaagaag aaatcgaatt agccaaacgt attgaacaag gtgatgaagt agcaaaatca
     9781 agacttgcag aagcgaactt acgtttagtt gtaagtattg ctaaaagata cgtaggtcgt
     9841 ggtatgttat tccttgattt aatccaagaa ggtaatatgg gtcttattaa agctgttgaa
     9901 aaatttgact ttaacaaagg atttaagttt tcaacatatg caacatggtg gattagacaa
     9961 gcaatcactc gtgcaattgc tgaccaagca cgtacgattc gtatccctgt gcatatggta
    10021 gaaacaatta ataaattaat tcgtgttcaa cgtcaattat tacaggactt aggtcgagat
    10081 ccagcaccag aagaaattgg tgaagaaatg gatttaccag cagaaaaagt tcgtgaaatt
    10141 ttaaaaattg cgcaagaacc tgtttcatta gaaacaccaa ttggtgaaga agatgatagt
    10201 catttaggag actttattga ggatcaggaa gcacaaagtc cttcagatca tgctgcttat
    10261 gaattattaa aagagcaatt agaagatgtg cttgatacat taactgatag agaagaaaat
    10321 gtattacgat taagatttgg tcttgatgac ggcagaacaa gaacacttga agaagttggt
    10381 aaagttttcg gtgttacacg tgaacgtatt cgacaaattg aagcaaaagc acttagaaaa
    10441 ttaagacatc caagtcgtag taaacgtttg aaagacttta tggattaatt tatatcaatt
    10501 ttaatgattg ataccaagac atgaataagc gcttatttat ttttagatta agatttaact
    10561 tgagtgaggt tggtacattg aataatgttc aacctctttt ttcataaagg agaataaaat
    10621 gatttcgtta aataaccgat taacgacggt aagtcgattt ttaaaacaag gtacaatcgc
    10681 ggatattggc tcagatcatg cttatttgcc aatctatgca attcaaaacc atttatgcga
    10741 atgcggtatt gctggagaag ttattcaagg cccttttcaa gctgctgtga aaaatgtagc
    10801 tgcaaaccaa ttagtagata gaattgatgt acgtttaggg gatggcttaa gtgttattca
    10861 acctgaggac gtaatcgata atatcacaat ttgtggtatg ggcggaccac ttattgctaa
    10921 aattttaaaa gatgggcaag ataagttaag tcaacatcca aggttgattt tacaaagtaa
    10981 tatccaaact gaaaatttaa gacaaacatt acaacaattg aattatgaaa taattgatga
    11041 aataataatg gaagaaaaag gccatattta tgaaattgtt gtagcagaat attctacaga
    11101 attaatagag ctatcatctg atgaattgaa attcggacca aaattgctga acaacaaaaa
    11161 tgaatatttt ataaaaaaat ggcaaagaga gttagaagca ctttatcata ttaaatcgaa
    11221 actcaatact gaacaacatc atcaacgttt agctcaaatt aatgacgaaa ttgctgtaat
    11281 tgaaagggtg ttatagtaat gaaaatagct gatttaatga cattgttaga tcatcatgtg
    11341 ccatttagta ctgctgaatc ttgggataat gtaggattgt taataggtga tgaagatgtt
    11401 gaagttactg gtgttttaac agcattagac tgtacgttgg aagtagtaaa tgaagcaatc
    11461 gaaaaaggtt ataatactat tattagtcat catcctctaa tctttaaagg cgtaacatca
    11521 ttaaaagcta atggttatgg tttgatcatt agaaaactaa ttcaacatga cattaattta
    11581 atagcgatgc atacaaattt agatgtaaat ccgtatggtg tcaatatgat gttggcgaag
    11641 gcgatgggtt tgaagaacat ttcaataata aataatcaac aagatgtata ctataaagtt
    11701 caaacatata tacctaagga taatgttgga ccatttaaag ataagcttag tgaaaatgga
    11761 ttagcgcaag aaggtaatta tgaatattgt ttctttgaaa gtgaaggaag agggcaattc
    11821 aaaccagttg gtgaagctaa tccaacaata ggacaaattg ataaaattga atatgtagat
    11881 gaagttaaaa ttgaatttat gatagatgca tatcaaaagt caagggctga gcaattaatt
    11941 aaacaatacc atccatatga aacaccggta tttgatttta ttgagataaa acaaacatcc
    12001 ctttatggac ttggcgttat ggcagaagtg gataatcaaa tgacattgga agatttcgca
    12061 gctgatatta aatctaaatt aaatatccca agtgtccgtt ttgttggtga gtctaatcag
    12121 aaaattaaac gtattgcaat tattggtggt tcaggtattg gatatgaata tcaagctgtc
    12181 caacaaggcg cagatgtctt tgttacaggt gatattaaac atcatgatgc cttagatgct
    12241 aaaattcatg gtgtgaattt aattgatatt aatcattaca gcgaatatgt gatgaaagaa
    12301 ggtttaaaag cgttactaat gaattggttc aatacagaaa aaataaattt agatgttgaa
    12361 gcatctacaa ttaatacaga tccatttcaa tatatttaaa ctaaaatctc gggatttcta
    12421 aattttgaaa tttcgaggtt ttaatatttt tatttaaaat agcacattta tactttataa
    12481 tagtaaagat gaacatataa ggaggccaaa tcatggcaaa acatccattc gaacaattta
    12541 atctagaatc tagtttaatt gacgctgtga aagaccttaa ttttgaaaaa ccaactgaaa
    12601 ttcagaatcg aattattcca agaatactaa agagaacaaa tttaattggt caatctcaaa
    12661 cgggtacagg gaaatctcat gcatttttat taccattaat gcagttaatt gatagtgaaa
    12721 taaaagaacc acaagcaatc gtagttgcac caacaagaga acttgcacaa caactatacg
    12781 atgcagcgaa ccatttaagc caatttaaag ctggtgtttc agttaaagtt tttattggtg
    12841 gtacagatat agagaaagat agacaacgtt gtaatgcaca accacaattg attataggca
    12901 cccctactag aattaatgac ttagctaaaa cgggacattt acatgtgcac ttagcatcat
    12961 atttagttat tgatgaagcg gatcttatga ttgacttagg attaattgaa gatgtagatt
    13021 acattgctgc aagattggaa gataatgcaa atattgcggt gtttagtgct acaatcccac
    13081 aacagttaca accattttta aataaatatt taagtcatcc agaatatgta gctgtcgaca
    13141 gtaaaaaaca aaataaaaag aacatcgaat tctatttaat acctactaaa ggtgcagcta
    13201 aagttgaaaa gactttaaat ttaattgata tactaaatcc atacttatgt attattttct
    13261 gtaatagtag agataatgca aatgatttag cacgttcact aaatgaagct ggtattaaag
    13321 ttggtatgat tcatggtggc ttaacgccac gtgaacgtaa acaacaaatg aaacgtatac
    13381 gtaatttaga attccaatac gttattgcca gcgatttagc atctcgtggt attgatattg
    13441 aaggtgttag tcatgtcatc aattttgatg tgccaaatga tattgacttc tttacgcata
    13501 gagtcggacg aactggtcgt gggaattata aaggtgtagc aattacgctt tatagtcctg
    13561 atgaagaaca caatatttca ttaatagaag atcgcggttt tgtattcaat actgttgata
    13621 ttaaagatgg tgagttaaaa gaagttaaag cgcacaatca gcgtcaagca agaatgcgca
    13681 aagatgacca tttaactaat caagtgaaga acaaagttcg aagtaaaatt aaaaacaaag
    13741 ttaaaccagg ttataagaag aaatttaaac aagaagttga aaaaatgaaa cgtcaagagc
    13801 gtaagcaatt tagtaagcag caaaatagac aaaaacgtaa gcaaaacaaa aaaggttagg
    13861 tgtgttttat gttattagga tcacatgttt caatgagtgg taaaaagatg ttagaaggtt
    13921 ctgctataga agcgcatgaa tatggtgaaa caacatttat gatttatact ggtgcacctc
    13981 aaaacacgcg ccgtaaaagt attgaagatt taaatataac taaaggtcat gaagtgatgg
    14041 aaaaatatgg tttatctaat attgttgttc atgcaccata catcattaat attgcaaata
    14101 caaccaaacc agaaacgttt aatcttggtg tagatttctt gcaacaagaa attgaaagaa
    14161 cgcaagctat aggggctaaa gatattgtat tacatccagg tgcgcatgtt ggagctggtg
    14221 ttgatgccgg aattaataag attattgaag gattaaatga agttttaacg aatgacaata
    14281 atgtacgtat tgcgcttgaa acgatggcag gtaaaggtac agagattggt cgctcatttg
    14341 aagagttagc acgtattatt gatggagttc ataataacga acgtttatca gtatgttttg
    14401 atacatgtca tacgcatgat gctggatata atgttaaaga ggactttgat ggcgtattaa
    14461 atgaatttga taaaatcatt ggagtcgaca gaatcaaagt tgttcatgtc aatgattcta
    14521 aaaatgaccg tggcgctcaa aaagaccgac atgaaaatat tggcttcggt tacattggtt
    14581 ttgatgcatt aaattacatt gtgcatcatg atagttttaa agatattccg aaaatcttag
    14641 aaacacctta tgttggtgaa gataagaaaa ataaaaaacc gccatataaa cttgaaattg
    14701 aaatgttaaa acaacagcaa tttgacccag aattaaaaaa taaggttatg caacaataaa
    14761 atgataatgc tctcttcaag aaatgcgata aataaaactt gaagggggca tatctcttta
    14821 ttttgtctaa ttttgagtcg taaacattac tgtttacata tgttactagg agttgtatag
    14881 ttgaatgcga ggtgagaata cgatgacacc agtctttgaa ttgaaaaatg tcaattacta
    14941 ctatgatcat aaaaaagtgt tagaaaatat aaacattaaa ataaataaag gtgaattttt
    15001 agcaattgtt ggaccaaatg gtgctggtaa atcaacatta ttgaagttga ttctagggtt
    15061 attaccttta caaagtggtg agatttttgt tgaaggtatt gattttaaaa ataagaaaac
    15121 atcaattaaa ttaagctatg tatcacaaaa agcaaatgcc tttaattcag gtttcccagc
    15181 aagtgttaaa gaagttgttt taagcggatt aacaaagaca aaacgtcttt tccaaacatt
    15241 taatagcaaa gataatgaaa aagtgattaa agtactagaa agactgaata taagtgattt
    15301 aattcataaa aatatagcag aattatcagg tggtcaacaa caacgtgtaa tgattgctcg
    15361 agcattgatt tcagaacctg cagtattagt acttgatgaa ccaacgaatg gtattgatgc
    15421 aaaacatgta agtgaatttt ataatacttt agatcaatta aaacaagaag gtatcaccat
    15481 tatcttagtt actcatgata tcggtgttgt agcagatact gctactgaag tagcatgttt
    15541 aaataagcat ttgcatttcc atggtacaac tgatgagttt aaatcacttg atgaagttga
    15601 aatttcaaaa atttatggac atcctgtacg ttttgtcgat catcagcata atcgagaatg
    15661 ttgtaattaa taaagatttg agtttattta aaatggaaag gcgtgtcaca attgatagat
    15721 gcattattga attttgattt tatgaggtac tctttaatta gtggtatctt gataggtttt
    15781 attgcgcctc taatcggtgc ttttatcgtt gttagacgac tatcacttat agctgatgct
    15841 ctaagtcatg taactttagg tggtatatct ttcggtatgt ttttacttac tattatgcca
    15901 acactagtat ttattaatcc aatgtggttt ggaatcttat tcgcaatagt aggtgcgctt
    15961 ctaattgaaa aattaagaac gtcatacact gcttaccaag aaattgctat tccaattata
    16021 atgagtgctg gtatcgcctt gagtgcaatc ttcatttcat tagctgatgg gttcaatcaa
    16081 gaaattgttg gattgctatt tggctcaata agcgcagtga atataagtga tttaacaacg
    16141 attattgtca ttgccatcat agttgttctg tttattacat tgttttataa agaattattt
    16201 atattgtcat ttgatgagga atatagtaaa gtcattggca ttcctaaatg gattcaattt
    16261 ttatttattg ttattgtagc gatggttatt tctgcctcaa tgcgtgttgt agggatactg
    16321 ttagtaagtg cgttaatcac gttaccaatt gcaataagta tgcgaataac aaaaagtttt
    16381 aagcaattga tattactaag tgtattttta ggtgaattat cagtaatatt aggcttagtc
    16441 ctagctttct atatggacat ttctccgggt ggtgtaattg tagttctact tgttatttta
    16501 cttatgatta caatggctta tcagaaaatg cgaatgaagt ttaaaaaggg agctaatatc
    16561 aatgaataca aatgatgcta ttaaaatttt aaaagagaac ggtttaaaat atacagataa
    16621 acgtaaagat atgttagata tttttgtcga agaagataag tatataaacg caaagtatat
    16681 acaacaagtt atggatgaaa attatcctgg aatttcattc gacacaatat atagaaacct
    16741 gcacttattt aaagatttag gaattattga aaatacagaa cttgatggtg aaatgaagtt
    16801 tagaatcgct tgtacaaacc atcatcatca tcattttatc tgtgaaaagt gtggagatac
    16861 aaaggtaata gattattgtc caatagatca gataaaatta tcactacctg gtgttaatat
    16921 tcacaaacac aaacttgaag tttatggtgt atgtgagtct tgccaagatt aatataaaga
    16981 aatgagattt atgcacattt ggtcagatgt atgcataaat ctcatttctt tatattatag
    17041 aagataaagg ttcatttcat actccgttcg tatgatttta tggcaaatct gttaactttt
    17101 tatatacaaa atgtttaatt atttttgatt tgagttatat taaatgagta gagaaaataa
    17161 taagtagtag aatttaagca aattctttgt tgaaccagtc actgcttgtt attatttact
    17221 tacagacatt ttaggaggat gattatttat ggcttttgaa ttaccaaaat taccatacgc
    17281 atttgatgca ttagaaccac attttgacaa agaaactatg gaaattcacc atgacagaca
    17341 tcataacacg tatgttacga aattaaatgc tgcagtagaa ggtacagatt tagaatctaa
    17401 atctattgaa gaaattgttg ctaatttaga cagtgtacca gctaacatcc aaactgctgt
    17461 acgtaataat ggcggtggac atttaaacca ttcattattc tgggagttac tttcaccaaa
    17521 ctcagaagaa aaaggtactg tagtagaaaa aattaaagaa caatggggtt ctttagaaga
    17581 atttaaaaaa gaatttgctg acaaagcagc tgcacgcttt ggttcaggtt gggcttggtt
    17641 agtcgtaaac aatggccagt tagaaattgt gactacacca aaccaagata atccattaac
    17701 tgagggtaaa acacctattt taggtttaga cgtatgggaa cacgcttatt acctaaaata
    17761 tcaaaacaaa cgccctgact acattggcgc attttggaat gtagttaact gggaaaaagt
    17821 tgacgaatta tataatgcaa caaaataaag tatgattact tgttcggtct cttaaatgag
    17881 accgttattt ttttgtcaaa aagatagaaa taatttctaa attcatatat gatttaaagt
    17941 gaaagacttt gaatagaggt aggtagtttt gttaaaaaga ctaaaagaaa aatcaaatga
    18001 tgaaatcgtt caaaatacaa ttaacaagag aattaacttt atatttggtg tgattatatt
    18061 tatttttgca gtactagtac tacgtttagg ttatttacaa atcgcacaag gctcacatta
    18121 taaacaaatt ataaaaaatg atgaaaacat tacagtgaat gagtctgtgc caagaggtcg
    18181 tattttagac agaaatggga aagttttagt tgataatgct tctaaaatgg ctattacata
    18241 tactaggggt cgaaaaacaa cacaatcgga aatgttggat acggctgaaa agttatcaaa
    18301 gctaatcaag atggatacta agaaaattac agaacgtgat aagaaagatt tctggattca
    18361 gttgcatcct aaaaaagcaa aagcaatgat gacaaaagaa caagctatgt tagcagatgg
    18421 aagtattaaa caagatcaat atgataaaca actgttatcg aaaatcggaa aatcacaatt
    18481 agatgaattg tcttctaaag atttacaagt tttagctatt tttcgagaga tgaatgcagg
    18541 aacagtttta gatccacaaa tgataaaaaa tgaagatgtc agtgaaaaag agtatgcagc
    18601 agtttctcag caactttcca aattaccagg tgttaacacg tctatggatt gggatagaaa
    18661 atatccatat ggcgatactt taagaggtat attcggagat gtatcgacac ctgctgaagg
    18721 tattccaaaa gaattgacag aacattactt atccaaagga tattcacgca atgatcgtgt
    18781 tggaaaatct tacctagaat atcaatatga agatgtattg cgtggtaaga agaaagaaat
    18841 gaaatacaca acggacaaat ctggtaaagt tacatcttca gaagtgttaa atcctggcgc
    18901 tcgcggtcaa gatttgaaat taacgatcga tatagatctt caaaaagaag tagaagcatt
    18961 attagataaa caaattaaga agcttcgcag tcaaggtgcc aaagatatgg ataatgcaat
    19021 gatggttgta caaaatccta aaaatggaga cattcttgcg cttgccggaa agcagattaa
    19081 taagagtggt aaaatgactg attatgacat tggtacgttt acttctcaat ttgcggttgg
    19141 atcttctgta aaaggtggaa cattattagc cggttatcag aataaagcta tcaaagttgg
    19201 agaaacaatg gtcgatgaac cattacattt ccaaggtggt ttgacaaaac gatcatactt
    19261 caataaaaac gggcatgtaa ctattaatga taagcaagct ttgatgcatt catcaaacgt
    19321 atatatgttt aaaacagcat taaaattagc gggagaccct tattattctg gtatggcttt
    19381 accttcagac ataagttcac ctgcccaaaa gctaagaaga ggattaaatc aagtaggctt
    19441 aggtgtgaaa acagggatag atttaccaaa tgaaacaaga ggtcaaatcg aaccattaac
    19501 aaataatcca ggtaattatc tagatttatc aattggtcaa tatgatacct atacaccatt
    19561 acaattatca caatatgttt caactatagc gaatgatggt tatagaatac agccacacat
    19621 tggattaacg attcatgaat caactaataa agatgaggtt ggtccactca agaagaaaat
    19681 taatggcact gtcttgaaca aggttaataa tactgaaaag gaaatcaaac aaattcaaga
    19741 aggattcaaa atggcattta atgataaaga tggtactgga tatgttagtt ttaaagatac
    19801 agtagtacct actgctggta aaacgggtac cgcagaagtg ttccaaaacg gagagccaag
    19861 agttaactct acttatatag gatacgcgcc aattgatgat ccaaaattag cgttttcaat
    19921 tgtatataca aatcagcctg taccaccacc atggttaaca ggtggagact taggtagaga
    19981 tgtaattaac tactacttta agcagttagg taaaaatgat aaaaataaag acaaagacaa
    20041 ataaaattta acctgacgat tgtgtagcgc atggttgtaa aattttaact ttgcaaatat
    20101 tatagatgtt ggtataataa taaagtcgta tttagaaatg ataaggagga ataaccatgc
    20161 gcgtaaacgt aactttagct tgtacggaat gtggtgacag aaactacatt acaactaaga
    20221 acaaaagaaa taatccagaa cgtgttgaaa tgaagaaatt ctgttcacgt gaaaacaaac
    20281 aaactttaca ccgtgaaaca aaataatcat taaaattaaa tttaaattaa caataaaaaa
    20341 ggtgaaatct caaaataaga ggtttcacct ttttaattat gcacacgtat gaaatgataa
    20401 aagattttca attgaaataa taaattttag aattgtcgca taattcatca tgacaacata
    20461 atgacatgta ttgttattaa cgatataata gaattgaata aagtgggtga taatgtgact
    20521 aaaaatgaga ttagaaaata cattttacat aaaatgaaga attttaataa agctgaaaag
    20581 cgaaaagcag acacatggtt aagaaatcaa ttttttgcaa ctgaagaata caaagaagca
    20641 aacgcaattg cgctagttct ttcttttaat catgaagtag atactttttc tattattgaa
    20701 caagccttaa tggatcataa acgtattttt gtaccgaaaa tggattattt aaatcatcaa
    20761 atgactttta aagagatatt taatctcaaa gatattgatg tcgataataa ggggatttac
    20821 tatccaactt caaaaggtga aacaacgaat aacctagatt taattgttgt tcctggtgtt
    20881 ggatttcaag acgatggata tagaattggg tatggtggtg gctattacga caggttttta
    20941 gctaattatc agacaaagac aataagctta ttatacgatt ttcaaataac atcatttgaa
    21001 ccggaatcat tcgatcaacc agtcgataaa ttgattatat atcaagcagc atagtggagg
    21061 actaaatgaa catagacaaa caattttgga aaacaatata ttattggatt aggtacttga
    21121 attttgatat tgtcagtagg gaaaaagatg accaagaaat ttggttagcc cataaaagaa
    21181 aaaaacaagt agtcattttt aaacaacata tcaagtctac ccaagaaata cgttttgaca
    21241 aagcgaaagt gcttgaacac aaagatgaaa tagcaaattt tatttctttc gaaccacaaa
    21301 gttttgaatt ttattatttt acagaatcgg aattttcaga agaacaatta aatgaagttt
    21361 cgccaattag aattaaattc aatgttataa gacacacaaa agatttgata aagcatatgc
    21421 cgaatatatt tttggctaga cttatttcag aagataatga taaaaagaca tatatgttct
    21481 ataaacgcaa agtattaacc gataactttt tagataaata tatgcagaaa ttttcaccgg
    21541 caacatacac aata
//
LOCUS       DAKTLH010000030        21219 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.030, whole genome shotgun sequence.
ACCESSION   DAKTLH010000030 DAKTLH010000000
VERSION     DAKTLH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21219
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.030"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            108..1967
                     /locus_tag="O0394_000990"
     CDS             108..1967
                     /locus_tag="O0394_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HCY0499080.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDIDKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKAQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            complement(2234..2794)
                     /locus_tag="O0394_000991"
     CDS             complement(2234..2794)
                     /locus_tag="O0394_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCY0499081.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(2962..4869)
                     /gene="sasF"
                     /locus_tag="O0394_000992"
     CDS             complement(2962..4869)
                     /gene="sasF"
                     /locus_tag="O0394_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HCY0499082.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMATSIILTNILPYDAQAASEKDTE
                     ITKEILSKQDLLDKVDKAIRQIEQLKQLSASSKEHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQT
                     ATTKHGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDASKDTSEDPAVSTTDNNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEAVKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSDLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            complement(5111..6469)
                     /gene="gtfB"
                     /locus_tag="O0394_000993"
     CDS             complement(5111..6469)
                     /gene="gtfB"
                     /locus_tag="O0394_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HCY0499083.1"
                     /translation="MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFALFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            complement(6462..7970)
                     /gene="gtfA"
                     /locus_tag="O0394_000994"
     CDS             complement(6462..7970)
                     /gene="gtfA"
                     /locus_tag="O0394_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HCY0499084.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISLAPTTYTLDDIERELGHDISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDIVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNQDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPTLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYND
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVIAKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            complement(7988..10378)
                     /gene="secA2"
                     /locus_tag="O0394_000995"
     CDS             complement(7988..10378)
                     /gene="secA2"
                     /locus_tag="O0394_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HCY0499085.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENQDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            complement(10368..11327)
                     /gene="asp3"
                     /locus_tag="O0394_000996"
     CDS             complement(10368..11327)
                     /gene="asp3"
                     /locus_tag="O0394_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HCY0499086.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQYDLEYKSMRVTKIG
                     DDQYGNDMINVIIAEPSDTYPTISNDFLKLFGHVWLVERWMDDNIEGNIKQLKNDLQS
                     QDTLKAINLISYGSKSNVFATYVAQHLGCKVYRTSHEDDDLKGWLTEHVPGNHELKDT
                     NVEVYFKEEQDKHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            complement(11305..12873)
                     /gene="asp2"
                     /locus_tag="O0394_000997"
     CDS             complement(11305..12873)
                     /gene="asp2"
                     /locus_tag="O0394_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HCY0499087.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIVLT
                     DDMLDSPLEIPAKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            complement(12863..14416)
                     /gene="asp1"
                     /locus_tag="O0394_000998"
     CDS             complement(12863..14416)
                     /gene="asp1"
                     /locus_tag="O0394_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HCY0499088.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            complement(14427..15638)
                     /gene="secY2"
                     /locus_tag="O0394_000999"
     CDS             complement(14427..15638)
                     /gene="secY2"
                     /locus_tag="O0394_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HCY0499089.1"
                     /translation="MLKLLQQYEYKIIYKRMLYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKSISDDMPMLTF
                     DSPVGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGLALVTVIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLNQYW"
     gene            complement(15768..21113)
                     /locus_tag="O0394_001000"
     CDS             complement(15768..21113)
                     /locus_tag="O0394_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
                     /protein_id="HCY0499090.1"
                     /translation="MSKRQKAFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGHGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSTADNAAKLNVQPTNNTFQDFDINYNGDTKVMTVKYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPIVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTISIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSDSASKSTSLSDSISNS
                     SSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDS
                     TSNAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSQSTSQSGSTSTSTSTSASVRTSESQS
                     TSGSMSASQSDSTSTSLSDSQSTSRSTSASGSASTSTSTSDSRSTSASTSTSMRTSTS
                     DSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDSTSASTSGSMSVSISLSDSTSTSTSAS
                     EVMSASISDSQSMSESVNDSESVSESNSESDSKSMSGSTSVSDSGSLSVSTSLRKSES
                     VSESSSLSCSQSMSDSVSTSDSSSLSVSTSLRSSESVSESDSLSDSKSTSGSTSTSTS
                     GSLSTSTSLSGSESVSESTSLSDSISMSDSTSTSDSDSLSGSISLSGSTSLSTSDSLS
                     DSKSLSSSQSMSGSESTSTSVSDSQSSSTSNSQFDSMSISASESDSMSTSDSSSISGS
                     NSTSTSLSTSDSMSGSVSVSTSTSLSDSISGSTSVSDSSSTSTSTSLSDSMSQSQSTS
                     TSASGSLSTSISTSMSMSASTSSSQSTSVSTSLSTSDSISDSTSISISGSQSTVESES
                     TSDSTSISDSESLSTSDSDSTSTSTSDSTSGSTSTSISESLSTSGSGSTSVSDSTSMS
                     ESNSSSVSMSQDKSDSTSISDSESVSTSTSTSLSTSDSTSTSESLSTSMSGSQSISDS
                     TSTSMSGSTSTSESNSMHPSDSMSMHHTHSTSTSRLSSEATTSTSESQSTLSATSEVT
                     KHNGTPAQSEKRLPDTGDSIKQNGLLGGVMTLLVGLGLMKRKKKKDENDQDDSQA"
BASE COUNT         6491 a         4035 c         3257 g         7436 t
ORIGIN      
        1 ttagtcgatt gatacctatc aactattttc agcatacgat aaattataac aaatcatagt
       61 ttattatcac acttaattat tatatttttc aagggagaat acgaaatatg cctaaaaata
      121 aaattttaat ttatttgcta tcaactacgc tcgtattacc tactttagtt tcacctaccg
      181 cttatgctga tacacctcaa aaagatacta cagctaagac aacatctcat gattcaaaaa
      241 aatctaatga cgatgaaact tctaaggata ctacaagtaa agatattgat aaagcagaca
      301 aaaataatac aagtaaccaa gacaataacg acaaaaaatt taaaactata gacgacagca
      361 cttcagactc taacaatatc attgatttta tttataagaa tttaccacaa accaatataa
      421 accaattgct aaccaaaaat aaatacgatg ataattactc attaacaact ttaatccaaa
      481 acttattcaa tttaaattcg gatatttctg attacgaaca acctcgtaat ggcgaaaagt
      541 caacaaatga ttcgaataaa aacagtgaca atagcatcaa aaatgacact gatacgcaat
      601 catctaaaca agataaagca gacaatcaaa aagcacctaa atcaaacaat acaaaaccaa
      661 gtacatctaa taagcaacca aattcgccaa agccaacaca acctaatcaa tcaaatagtc
      721 aaccagcaag tgacgataaa gcaaatcaaa aatcttcatc gaaagataat caatcaatgt
      781 cagattcggc tttagactct attttggatc aatacagtga agatgcaaag aaagcacaaa
      841 aagattatgc atctcaatct aaaaaagaca aaaatgaaaa atctaataca aagaatccac
      901 agttaccaac acaagatgaa ttgaaacata aatctaaacc tgctcaatca ttcaataacg
      961 atgttaatca aaaggataca cgtgcaacat cattattcga aacagatcct agtatatcta
     1021 acaatgatga tagcggacaa tttaacgttg ttgactcaaa agatacacgt caatttgtca
     1081 aatcaattgc taaagatgca catcgcattg gtcaagataa cgatatttat gcgtctgtca
     1141 tgattgccca agcaatctta gaatctgact caggtcgtag tgctttagct aagtcaccaa
     1201 accataattt attcggtatc aaaggtgctt ttgaagggaa ttctgttcct tttaacacat
     1261 tagaagctga tggtaatcaa ttgtatagta ttaatgctgg attccgaaaa tatccaagca
     1321 cgaaagaatc actaaaagat tactctgacc ttattaaaaa tggtattgat ggcaatcgaa
     1381 caatttataa accaacatgg aaatcggaag ccgattctta taaagatgca acatcacact
     1441 tatctaaaac atatgctaca gatccaaact atgctaagaa attaaacagt attattaaac
     1501 actatcaatt aactcagttt gacgatgaac gcatgccaga tttagataaa tatgaacgtt
     1561 ctatcaagga ttatgatgat tcatcagatg aattcaaacc tttccgtgag gtatctgata
     1621 gtatgccata tccacatggc caatgtactt ggtacgtata taaccgtatg aaacaatttg
     1681 gtacatctat ctcaggtgat ttaggtgatg cacataattg gaataatcga gctcaatacc
     1741 gtgattatca agtaagtcat acaccaaaac gtcatgctgc tgttgtattt gaggctggac
     1801 aatttggtgc agatcaacat tacggtcatg tagcatttgt tgaaaaagtt aacagtgatg
     1861 gttctatcgt tatttcagaa tccaatgtta aaggattagg tatcatttct catagaacta
     1921 tcaacgcagc tgccgctgaa gaattatcat atattacagg taaataagta ttatattaaa
     1981 cccgtaaaat ttataagtat aaacaaggag ttcggactta aacatatttc tgttcataag
     2041 tctgatttct tattcaatta aacccgaggc attcagttcg aacgcctcgg gtcgttttat
     2101 ataaatatat tattttatgt tcaaatgttc ctcatcatat ccgtttcaat tgccatctca
     2161 cacattttat aaatatgagc aaatgtactt attctcaaac attactgccg agctttaatt
     2221 gacgttatat taactataaa ctacttttcc atgactctac ggattcaatg tcacatgagc
     2281 gtgataaaat ttgttcaata ataaagtcat gtttatcatc tgatctatca ccaacagcat
     2341 cttctaaaac agtaatataa tagtctttat ctacactttc taatgccgtg ctcaatacag
     2401 ctccacttgt agagacaccc gttaatacta aatgattaat atcatttgca cgtaaataaa
     2461 cttccaagta actacctgta aatgcgctaa agcgtcgctt agaaataatc ggctcatctt
     2521 ctagtggtgc taaatcttca agtattcgtg tagatgcatc tgcttcagta atcgcatatc
     2581 cttgagcttt aattgttgaa aacactttat tactcgagga gacatcatta aaatgcttat
     2641 ctaacactaa acgtatgaaa atgactggta ttcgatgttg tcttgctgct tcaattgctc
     2701 tctgattcgc tttaataata ttttttattc taggtacact actcgctata ccttcttgca
     2761 tatccaaaac taatagcgcc gtttttcgag acatcttcat tctcctttac ttgtgtagtt
     2821 ctaagtcgtt aaattcatta taacgttaaa atgatggaca atctattcat tgcattttgc
     2881 atatacttca caataaatta aggggaaata agacgtctta tatacttaaa aaaatatata
     2941 gatgctcttc ccccaatata attatgcttt atttttcaac ttattgcgtc gtgataacca
     3001 aatcattagt acacccattg caccaacaat tacagatatc ggcaaccaat gttcttttat
     3061 cgtttccccc gctttaggca aagatacatt accatcagca tttaataatc cacttaacaa
     3121 tccattacct ttaccaagtg ttaacgtctt ttctggcttt ggtgtgggta tatctggaat
     3181 actgtctaat aaatctgatc cttgattcat taaatttgct aacttattta aatctgtcgt
     3241 tttcccattt ttattcaatc gatctagtaa acttggacga tttactattg gtgataaaat
     3301 ataatctata tcttttttcg tttgattgag tctcttttgt aaattcaata aatcatccgc
     3361 tttaccattc aatgccgatt taactaaatt aaaaatttta ttttgatctg tttctatttt
     3421 agtaattaaa tctgccagta attttgcctt ttgtctttct atacgtgttg ctaaaatcgt
     3481 ttcaattgct tgttttttat ctttggcatt attcaaaatt gcttttaata tatcatctga
     3541 agacgtgtcg ccagttgatg caaaatgttt cttcaattgg tcaacgattt ggcgatttga
     3601 taatccttta ttcgtccaat ctttagccaa tttatctgct tcagcttttc ctaatttcgt
     3661 ttgtaaaatt tgagaaatca atagcgactt atcttgtgat tgatcaatca atgacgttaa
     3721 taaatcatca ctcgttgtca gagataattg atcaatatga cgagtaattt gatctgcaat
     3781 ttgttgatct gttttaccat caacacgtat atcttttaga attttaactg cctcgtcttt
     3841 attaaatata ctttctaaaa tgctttgtgt agcatacttt ttatcatcag tacgtgcaag
     3901 ttcttccaaa ataatatttc gttgactttt tatacgctct ttcgtcttat ttacttcgct
     3961 cattaagtct gatttttgat ttttaggaag ttgcgtattt gcaatacgtt gatctaaaga
     4021 ttgtaacgta ttcagtttat gatatgtgta atgttgcgtt gaggcattac ttttagctaa
     4081 tttttcaatc atagcatgat taattttatc gcttccttgt aatttatcag tgagtcgatt
     4141 actatggctt tgattctctt catttgaaag gaatttattt aacacaacat gtccagaacc
     4201 atcattattt ggcgttttag ctacttcatg attattatct gttgtagaca ctgctggatc
     4261 ttccgatgta tctttcgatg catctttcga tttgtgtatt tgctgattca aatggtctag
     4321 gtcttctaac gccttattta ccattgcttc atcattttta tcatcttttt ctccatgttt
     4381 tgttgtagcc gtttgtgaca tatcattttt cattgcatta agatcgtcct cgccactttg
     4441 ttgaccccta tcaacatttg aagaaacctc atttaaatct ttaagcaatt gatctaattt
     4501 actgtctata tcactttgac cgttcatttc agtgtgagaa cttttattgt ctttgctatc
     4561 caactcatta gctcgtttta tgatttcatc tatttgcgat gctgttttcg cttcatttag
     4621 ttgtgcttta taatgttctt tagatgaagc cgataactgt tttaattgct caatttgacg
     4681 aattgccttg tcaactttgt ctaataaatc ttgcttagat aatatctctt ttgtaatttc
     4741 agtatccttt tcagatgcag cttgggcatc gtacggcaag atattcgtta aaatgatact
     4801 tgtcgccatc attgtcgaac acgataactt tacatataat tgaaacggtt tccctcgata
     4861 tttagccatc aacatactcc ttcctcactt acttccttca aagaattaca tactattata
     4921 tacctgttta caagaaattt acacttatct atctagttat tgttgttagt aattatctac
     4981 ttattactta gcttatattt aagtaaacaa aacaagcatg acgtaatatc atattgtcca
     5041 tgtcgctaac atcatattac gtcaaatctt ttatattaaa tgatgtttta ttttagactg
     5101 ctttttcctt ttagctttcg agcgcctgtt taaaaacttg ctcgaattgt tcacgcgaga
     5161 tttcgtgtgc atgtgctttt tgtgctaata aagcatctcg aaactgttgt tgatctttca
     5221 aactttctaa catttgtatt aattggtctt tactttccat tgttatctca tcattatgct
     5281 caaataagtg ctctgataat gttactttag catggtgtgc ggtttgacga taacctaaaa
     5341 tcaacaactc atagtcaaac gcttgttcca ccgcatttaa aatttcatta ccctcattga
     5401 tatcaagata aatatcacat aactggtata gttcatttac cctgtcaata ttaatagatg
     5461 ggtataaatg cacattagca tattgatcaa gttgcattag cttatcagac atctctgtaa
     5521 tagcagcgat gtgaaactta aaatctggta aagtttcaac caataccttg atgttacgaa
     5581 gttgatccga gttagttaat attacaattt ctttagtata tctattacga ctacgatagt
     5641 tatatagata tccgccttgt aaaatacgag attgaacctt tgcgtctgct atattgagca
     5701 tcgtttcata ttcgttttta tctggaataa taatattaca atgtcgtttc atatcacctt
     5761 tacacatcaa ttgcatattt cccgggacat taccattaca gtgttcttgc cataccaaaa
     5821 catcactacc ttttgatggc aaattatata acactgaaaa tggtagggct agtgagttaa
     5881 taacgaaatg atgttccgta atttcaagtt gcttgataaa aaataatgcg aatgcgagct
     5941 ttgaagggaa aaagtaagac ttcccttgcc aatccaatat gacatcagat gttacaaaat
     6001 tttcataaat cacttcttta ccttctgctg tcatatattt cttcaagatc gctttacgat
     6061 ttaaatcgta aacagtttgt gcaaatttaa taccattctt agaataataa tcgacaaatc
     6121 ggacacgttg ttggtcatca aaccattcga cacgactaac aattctaggg cgctctccac
     6181 tttgataaaa tattttacct cgtagacgtc ccatatcatt aattgtagcc gaattgttgt
     6241 tacctttaat ttcccaaaaa gctggtacag taacctgatt aaaaaatcgt ggtttcatat
     6301 tttctgtatt atgattatct gcaaaaaatt gatacggtga tataacatcg tccggtaaaa
     6361 agccattgtc attgagtaca attgttaaat cttcttccaa cttactggct ttaaaagact
     6421 catataactt tcgtgaatga tcgttaaagt aatcaaataa tttaatcatg tagcacctct
     6481 tgaactaatg tttcccattt taaaataata tcttgagtca taaattgctg tgccacttca
     6541 taagagatgt catgtggtgt ctggggacca ttgttaaaat acattacaat ggcatgagct
     6601 agttttgcga taacatcatc cacactatct tcgtcggtat caaaaggtac caagtagcca
     6661 ttttccccat ctcgaataaa ggttgggtta ccataattca catttaatcc aatcatacct
     6721 agtcctgagc ctaccgcttc cattagtgtt aacccaaaac cttcgctagt tgatgcagaa
     6781 agaaataact cataatcatt ataaatttca tcaagtttaa catgccctag taaacgaata
     6841 taatcttgtg cgcggtgtgt atcaataatt ttacgcagtc gcgtcttctc gctaccttct
     6901 ccataaatat caaatgttaa ttctggcact tgtcgtttag ccacgataac cgctttgaca
     6961 agccaatcaa tatgtttctc attcgctaaa cgagatgcac taatcatcgc atatggcttt
     7021 cttgataatg ttggatatga taatgcatca atgcttccca caggaatagt ataaacacgt
     7081 gggcgataac cttgatattg ctcaaattgt cgacaaacca tatgattttg aatatctgtt
     7141 gctgtaataa agaaatcaat gtatttagct tttgaaaatt gatattcata ataattgttc
     7201 catagtatat gctgctcact catcatatta ttactataat gatcagcatg aatcacaaca
     7261 ccaactttac tatcaccttt atgctgcaaa acagcctgac caatatcaga agcgcggtct
     7321 aatatgacaa tatcgtctcg ggttaaattc aatcgttgta aaaagtatgc aataaattcc
     7381 gttttgttat acaacaccgc atcttcaaac acatatatag agctgtctcc atcaatatat
     7441 tcgttataag cgatggaacc atcttgatta taaaattgtc gcatatataa tttcgcttta
     7501 ttatcagctg gtgcataata ctcagaaaat atgcgcgtat aactataaaa atctttacgt
     7561 actaacatac tattaattac aaattctgca cgatccacaa tatctttttg ttcattttgc
     7621 agataacatg ttacaaatga tgatttccca ttaaaatata ggcggactat cttaccattt
     7681 ctttctctaa aactaatgtc atgaccaagc tcacgttcaa tgtcatctaa cgtgtacgtt
     7741 gttggtgcta aagaaatatc actaaaatac tgatacaacc aaataacttc ttgatcttta
     7801 aacccaatgt tttgcgttaa tgtctgtatg ttctctgact gtataaaatc taaaaacaca
     7861 aatttagtgt cttgatttgt acgtctcaat aatttagcac ggtaagcttg tgcatattca
     7921 acaccgctac tcgcccagcc tataccaaag tttatattat atattgtcat gcgctacccc
     7981 ttttcattta tggaaaatgt ataactggca taccctcttt atcaaatgta atcatgcttt
     8041 gacaaatatt tttcaccatt ctttttttga tatttcgtgt cataacttca aatgaatcta
     8101 aggcaactct atggtattca aaaatagcgt tacgttgccc attttgtcgt tgattaacgc
     8161 ttgcttttaa ttgttgtaaa tagtcgactt gttctaacca acatgaatca attgctttca
     8221 aaaagacttt ttgaacgaaa atattataat aatatgcact ttgcatgttt ttacgattca
     8281 aagctaattg cttttcaaat tgctctaata aaaatgtcac tactgcttgc ttatctttaa
     8341 aattaacaca agccacatct ttattaaatt ggaaacttaa attttgataa atatactcga
     8401 caacacgcga ttttgttagc accttttcct catttacaaa catttcaaat acatctttag
     8461 ctaacgcttt aaaatcttga ttctcagcat catctatttc taaaactcga ttgcgttcct
     8521 cgtatacaag atctcgctgt atactaatgc ttttttcaaa ttcattagcc atttcacgag
     8581 ctttaacccc ttgttcttcc gagatacgct gcgctttaac tacaatttgc ttaactttgc
     8641 gattaaacaa attactttgc gataatcgtt gtgcatctaa tgaatataat tgattatttt
     8701 ccgctaaatt actatcgctc catcgcttaa ctaaataatc atctagtgaa atatatatac
     8761 aagatgatcc cggatcccct tgtctaccag aacgaccacg taattgcctg tctacacggc
     8821 tattttccat atgttcatga ataataacag ctaatccacc taatgcttcg acaccttcac
     8881 caagtttaat atctgtgcct cgacctgcca tactagtcgc aacagtcatg gaaccaattt
     8941 gccctgcttc agctatcatc tgcgcttctt ttgcaacatt ttgcgcaatg agtaaattat
     9001 taggaatatc catttggaat aatactttcg aaaagtattc agccgcttca gcagttctcg
     9061 ttatgagtaa aaccggtcgc cccgtttcat gaagttcaac tatatcatga atcatcgcga
     9121 tgtttttctc atcaactgaa cgaaacactt tatctggttc atcgatacgt tgaatcgctt
     9181 tatcagttgg tacttgtacg actatttttg aatacaaatc aaagaactct gattcgccta
     9241 attttcctgt agctgtcata cctgaaaatg attcaaaaag tttaaataaa ttctggaagg
     9301 taattgttgc cataacactt ttatctgttg aaacctccat accttctttc gcttcaatag
     9361 cttggtgaag tccagcttgc aacttagttc ccggtaacat acgacctgta atacggtcaa
     9421 ttaaaacaat atcaccatta tatacaaagt aatcgacatt agattcaaac aaatattgtg
     9481 cgcgcagtgc taaattaata ttacgcacta ggaccatcgc ttgttcgcta tataaatctt
     9541 caacattaaa gtatgattgt gccgcttcaa taccttgatt taacagccat atttcttttt
     9601 tggtcttctt cattttaaaa tgcacgtctt caatcaatgt atctacaaac tctttcacaa
     9661 tatgaaatag atttgattgt aatcttggtg cacccgaaat aactaatggt gtttgagcag
     9721 catctaaaat gattgaatcc acttcatcaa taataccgta atttaattgt ggtaaaaatt
     9781 tcccttccgc actatcagcc aaattatcaa ttaaataatc aaaaccgaga cgtccattag
     9841 ttgtatatat aatatcatgt tcatatatat tacgtttttc ccctttttga tactcataat
     9901 ccacaatatc aacaaaacct aatgaagcag ttaaacctaa ccattcatat aatggttgca
     9961 tctcttcaaa atctcgcttc gctaaataat cattcgttgt aattaaatat gttccttttc
    10021 ccgaaagagc atttaaatat aaaggcatcg ttgccgttaa tgttttacct tcgcctgttt
    10081 gcatctccgc aatgttacct tcatgcaata caatcgctcc gattaactga acttctttag
    10141 gatacatacc taatactctc cagctcgctt cacgtgccac tgcataagct tcaggtaaca
    10201 atgtatctag tgtatcaact cctgatgcta aacgttcttt aaattctatt gtcttttgtt
    10261 ttaacgcatc atcagaatat gatttaactt catcgctcca tgtattgatg cgtttcacta
    10321 tttttctaat cgactttagt cttaattcgt ttatcgtaac atctagttta tgtttcattt
    10381 acttccccac cattcagttt cgatacatct aagtaatcta aaaatcgtac tggattcatt
    10441 aaacgtgaca tataatttag atgtttgtct tgctcttctt taaaataaac ctcgacattt
    10501 gtatctttta gttcatgatt tcctgggaca tgttctgtaa gccatccttt taaatcatca
    10561 tcttcatggc ttgtacgata cactttgcaa cccaaatgct gagcgacata agttgcaaaa
    10621 acatttgact ttgacccata actaatcaaa ttaatagcct ttagggtatc ttgactttgc
    10681 aaatcattct ttagttgctt aatatttccc tcgatattgt cgtccatcca acgttcaacg
    10741 agccaaacat gaccaaacag tttcaaaaaa tcattcgaaa tagttggata ggtgtcagat
    10801 ggttctgcaa taatgacatt gatcatatca tttccatatt ggtcatcgcc tatcttcgtc
    10861 acccgcatgc ttttatactc taaatcatat tgatgcgtca tctctgtgat tgttaaacat
    10921 ctaaatataa gactcgtcga tgctgcattc atcattttta ttttataagc ataggcttca
    10981 tcaggatatt gaatcgtaat actatttgac tttacaatct cagtacttag ttttgtgcca
    11041 tttttattat aaaaaatgat gataaaatac actgaaccag caggcgttgc atcaaaatca
    11101 aaatgcaatt tataatgctg tcctctacgc aaaattggta aacttggcgc acttttatat
    11161 tttgaaaatt gctttaacat caaccactca tgaatcggta atccagaggg catcaaagga
    11221 tttataaaag tcacttcacc atttgaaaat gatactttag agccatacat aaatgtagtt
    11281 tgtgaaatat aattccaagt aactttaaat gttttgtttt tcagcatgtt gaactctccc
    11341 aaacttgtct tccaaaataa tgttgtaaaa attaacaaac caacttgcaa tggtaggtga
    11401 atcatcatta tgtcgcccag gaatactgcg attcatcact cttgcttggt gtgctgtcaa
    11461 tacaggtaat agctcttgaa atgcatgtgg atcataatca tcatgttgca tatatgctat
    11521 ggcaaaaaca gtttgtgaca atgatttctt ttgaaatgtt tgccaaaatt tttgatttaa
    11581 tgcctgtatc gacgcttgag atgtatcacc ttcattagac accaggacgt ctaatgctgt
    11641 accgaactct tctggtctaa gtaatcgcat atgttcagca atcgttccaa tattaacaag
    11701 tggtttacca acaataattg cctgaggatt taactgagca ccataataca aggcaccaaa
    11761 tgaacccata gataaaccag ataatattaa ttcatgtgat ttaaaattca gcttttctaa
    11821 tgtctcgtca ataacattga taataccttg ttcatattca gatgaaccga tataaaaact
    11881 accaccttca acacgaggat cgccgataag taaaaacggt gcattcatac gtttcatcat
    11941 ataatatcct tcgaaacctt ccgctgttcg ataaccacta aaatatacgt ttagtggcgg
    12001 tttcatatca ccagggtgga aataataaat aaattcctgt cgttgactat ctacgaaacg
    12061 actaccacca agtaaaaatt gacccatgtc taatctagac catcgtttgt gtataggtcc
    12121 taaatgtacc gtcccgttcc cacgcgcctt aacagttaca cttatataag catcaaatgg
    12181 tttcgcaggt atctctaaag gactgtctaa catatcatca gtcaatacga tttgttcaat
    12241 taatgcacca tcagcgccag tctgaatcaa tctaaatgta tattgcaact cgaccgcacc
    12301 atcaatatca aattctggcc atatttgaat gactttatct ttatcgtaaa cgagattatt
    12361 ttgccaagat gcgataggtt taaattcttt cccaaattct ccactcaatg tgagctctga
    12421 attaccttgg taaacgacat ctcctttaaa attcggatgc acaagtgcta acttaggaga
    12481 aaccttatct ccatactgtc ctgagaagct aactgcctct aatttattat tacgttcttc
    12541 aatattccgg taatgtaatg gttgaacaac gtatttttgg acattttcgt cttgttcata
    12601 ttcaactgac caaaatgatt catcaacata cgtattgtat ggttcgctta tcatttgtaa
    12661 taaattcgtt aatgtctccg agtatggtgc ttgaatatag ataaaatcaa agcgcccttc
    12721 tgcttcaaca atcgcttcaa tagcctctac ataaccacta tcaaattcaa acaatccaat
    12781 atcgaagtaa tcccaactca cacctttttt gtgttgaaaa ataggttcta aatcgtctcc
    12841 tccaatttgc aaaactctaa atttacgtgg catcattttc accttctatt aactcatcga
    12901 gctgattaat aatattctta gaagcatatg catctattaa ttttaaagaa taggcgtacg
    12961 cataattcca atttttcaaa taaaataaat aataatttaa cgcatcatct aattcatcaa
    13021 ctgtatttat aatacggcca ttgtcataat cagagacgta atctgtttgt tgaccattaa
    13081 tttgtggaat cccagcgcta attgcactaa tttgtaaata caagtcaggt tcttttgaca
    13141 tatctatcac aagtcgcaac gtccgcaatg cttctacaac atcatgttca gcatgtatcg
    13201 tcttaacagc aatgatgtca tcttgatctt caggtgtcat taatgctgaa acattaacat
    13261 ccgcattctg tttagcttgg tattcctcat ttaccgacgt aatacattca cgaagccaca
    13321 tcggtatgtc attttgatgg cgcgataata aaattaaacg gtaataatct tcctgtgcga
    13381 tataatccac aagtcgttgc atcatttgtt gcaaatcagc gtcactcata ccatctatcc
    13441 atacacctat aaatgtttcc atcaattgac tacttatatt aggtgattgt ctcgtttcaa
    13501 atggtgtgat tcgaatcatt gtattctccc gctgatactt ctcttgatga ctaattaaat
    13561 cacgttctag ttgcacacta tcaacaatta aatgggcatt ctttacgata gattgatatt
    13621 cctcatctga cacagtttca tttctatttt taaaaaatga ataacttaat gatttcgctg
    13681 gaatatgatt ggctatttgt cgattgtgcc tagcatctga agccacaatc acatgatcat
    13741 cttcatgtat ttgttgtgca atcattgctt gaaatttttc ttcaattagt tgagccatat
    13801 tgttatattc tgtttgttga tagtgatgtt gatatctttt tgaaacagtg actctgccat
    13861 ttttcaaatc ttcatgaagt acacaatctc cattaatcgt taaatattct tggtaagaag
    13921 cctctccctg atcatcaaaa taacgtatcg ctgataaata acctctgtca tcaaaaatat
    13981 aacgccgttg taactgatct ctttcaaatt cttcaaacca aattgaatac ccttcttgac
    14041 taaaataaat atttgtatag gtctgttcac tcgtcacaca ttttaataaa tacggtgtgt
    14101 acacaaactc aacatcatcc ggccatttta agtgatgata attaatcgct tgtggcgcat
    14161 ggtgactgaa tccttgaatt tcatcaaaca cagacgaata ctttgtctca tataagtcat
    14221 atcgatgtaa aaatgttctt aaatttggtg catgattgag aacaatcagt tgataatcta
    14281 agtcattttc aaggtgcatt cccattaaac taatcatatc gtcaaattcc gtcttatttt
    14341 gtagttgata atacggcaca gtcgtgtctt gccaccatcg ttggtcatcg taccaagctg
    14401 gaataaagta tttcataatt acctccttac caatactggt ttaaaaatgg cttatattta
    14461 tcaaaatata aatatgtacg aattgtttct gcaatattaa tactgatgta aactaataca
    14521 atcagttgta ctgagaaata aatttcagta gataaatgcg gtacaaacaa tgtgaaataa
    14581 agcggtatac caataatgac tgtaactaat gccaatccaa accaacatac gcgtcgtgct
    14641 tgataattta aataacgttc tgtatcctta ccaggtttaa ctcctgaaaa ataattgcca
    14701 ctctttaaga aatctttgga tttttgttta gtattgatta aaaatctcga taaaaaataa
    14761 cccaataaca tttgaatcac taaatatact gaaataccta ctggactatc aaatgtcagc
    14821 attggcatgt catctgatat gcttttatta aacatagata aaataaaatg aatgccactt
    14881 tttaagaaaa caaaagctga aatactcatc attaaagtaa tactgcctgc agggttaact
    14941 ttccaagata aataagattt catatttgtt gcggaaacgt tcattaaatc gatatatggt
    15001 attctcactt ctactaattc aataaataat aagataaaca atgtgattat cacaaggatg
    15061 attaacaacg caatcacaat atgacttgca tctatatatt ccattttttg atgcatcatt
    15121 gatttaataa tactaaccat tacaatcggc attggtcctg cgatgccgta gcgactattt
    15181 ttgtcagcta accaaactaa taacatcgtt ccagtaacca aaatcaatat tgttaagtaa
    15241 atattgtctt gatgaacacg ttctttcgaa acatattcat gaatcacaaa ataactttga
    15301 ataacactta aaattaatgt taagatgcgc tctttataat gcttctcaag acttgtttgt
    15361 ttcatgtatt tatccatatt ccgataagaa atcagcatca agataatcat tgatgttaac
    15421 cacggaccta atcctaaagt gaaaatgttt aaagtattaa cgtctccacc catattagaa
    15481 atagctattt taaaaaatga ctcatgtttt acttgcatat cgttatagga aacgatggaa
    15541 atgtttgtgc ctaatatata aataaacaag ataaaacatg tgtatagcat acgtttatat
    15601 ataattttat attcgtattg ttgtaaaagt tttaacatgt tgcacctctt ttatatcaaa
    15661 aacattaaaa agactaaggg ttcatcacta attattaaaa tcctatatcg atttttctag
    15721 tgattggtgc ctcagtcttt ttaattttag ccagctataa attcaattta tgcttgagaa
    15781 tcatcttgat cattttcatc tttctttttc tttctcttca ttaaacctaa accaactaat
    15841 aatgtcataa cgccacctag taatccattt tgttttattg agtcacctgt atctggcaat
    15901 cttttttcac tttgtgctgg tgtgccatta tgtttagtca cttcagatgt tgcacttaat
    15961 gtagactgag attcactcgt gctcgttgtt gcttcacttg ataagcgaga tgtgctcgtg
    16021 ctgtgagtat gatgcatact cattgagtct gacggatgca ttgagttaga ttcggatgta
    16081 cttgttgagc cggacatact tgttgatgtt gagtcagaaa tgctttgtga accagacata
    16141 gatgtactca gtgattcgga tgtgcttgtc gaatcggatg tgctcaatga cgttgatgtg
    16201 cttgttgaca ctgattctga gtcactaatt gatgttgagt cggatttgtc ttgtgacatt
    16261 gaaacactcg atgaattaga ttcactcatt gatgttgagt cagatacgct cgttgaacct
    16321 gaaccagacg tacttaatga ttcagatatg cttgttgaag ttgaaccact tgttgagtcc
    16381 gatgtacttg tcgatgtcga gtctgaatct gatgtactca atgattctga gtcactgata
    16441 gaagttgaat cacttgtaga ttctgattct actgtacttt gtgaaccact gatacttatt
    16501 gaagtagaat cactgatact gtctgatgtt gataatgatg tcgacaccga tgtgctttgt
    16561 gatgacgatg tactagcact cattgacatt gatgttgata tcgatgtact taaggaacca
    16621 gatgcacttg tacttgttga ctggctttgt gacattgaat cacttaatga tgtagatgtg
    16681 cttgttgagc tcgagtcact tacacttgtt gaacctgata ttgagtcact taaacttgtc
    16741 gatgttgaaa ctgatacgct tccgctcatt gagtcagatg ttgaaagtga tgtactcgtt
    16801 gaatttgatc cactgatgct agacgaatca cttgtagaca ttgagtcgct ttctgatgca
    16861 ctgatgctca tagagtcaaa ttgactatta cttgttgagc ttgactgcga atcgctcaca
    16921 cttgttgacg ttgattctga tccactcata ctttgcgagc tactcaatga ttttgaatca
    16981 cttaatgaat ccgaagtgct aagacttgtg gaaccactta aagatattga tccacttaat
    17041 gagtcggagt cacttgtact agtagaatca ctcattgata ttgaatcact tagcgaggta
    17101 gactcgctta cgctttctga accacttaat gatgttgagg tactcaatga accagatgta
    17161 cttgttgaag tcgaaccact tgttgatttt gaatcactta atgaatcaga ttcactcacg
    17221 ctttctgaac ttcttagtga cgtcgataca cttaatgatg acgaatcgct tgtgcttact
    17281 gaatcgctca tcgattgtga gcaactcaat gaacttgact cgcttacact ttctgatttt
    17341 cttaatgacg ttgagacgct caatgagcca gaatcactga cacttgttga gccactcatc
    17401 gatttagagt cactttcaga attagattca cttacacttt ctgaatcatt tacagattct
    17461 gacatacttt gtgaatcaga tatgcttgcg ctcattactt cactagccga tgttgatgta
    17521 cttgtcgaat cacttaacga tatagacaca ctcatcgaac cagatgtact cgcacttgtt
    17581 gagtctgatg ttgaatcact aacactatca gataatgacg ttgaatcact catacttgtt
    17641 gatgtacttg tcgaaagcga catactttgt gaatcactag tacttgtacg catcgaagta
    17701 ctagttgaag ctgatgtact acgagagtca cttgttgatg ttgatgtact tgctgatcct
    17761 gatgcacttg tacttcttga tgtgctttgt gaatcgctta atgatgttga tgttgaatcg
    17821 gattgacttg cactcattga accagacgta ctttgtgatt ccgaagtacg tactgaagca
    17881 cttgtcgacg ttgatgtact tgttgaacct gattgactag tactttgtga taatgactta
    17941 ctatcagaat cagatgtact ttgtgaatca cttaatgatt ctgatgtact acctgactga
    18001 gacgtgctca ttgaactact tacggacatt gatttactgt ctgatgcaga taatgaccca
    18061 cttgtactga tagagtcact tactatctct gaagtactca tcgagtctga tgtacttgtt
    18121 gagacacttt gtgatgctgc tatgcttagt gatccagaaa cagaaccact tgtgctcgtc
    18181 gaatcgctca atgattctga tgtactcatc gattttgaat cacttgtact taatgatatt
    18241 gatgtacttt gtgaatctga tttgcttgtt gacgcacttt gagagttggc tatgctattt
    18301 gaaatactga tagagtccga ggtgctagct gactcgctca atgatgttga tgtactaatt
    18361 gcattcgatg tactgtcact taatgatgct gatgtactag acgaccctga tatactcgtt
    18421 gataagcttt gtgacttaga caagcttcct gatgtactca tacttaatga gtcactgagt
    18481 gatgttgatg tacgcaatga atcagatgta cttgttgata gactttcgga tttttcagta
    18541 ctgctagagt ttgaaataga atcgcttaat gatgttgatt tactagctga atccgacatg
    18601 cttgatgata cactttgtga attcactaaa cttgtgcttg ttgagcttga tacactatta
    18661 ctttcagatg tgcttaatga cttagatgca ctcacagaat cagataggct tacacttgtc
    18721 gatttcgagg tactagctga tgtagatacc acaatcgatc ctgatgtact cgttgatgca
    18781 ctttgtgagt cagctttact tgttgacaca ctttgagatt gttgtgtact tcctgatgtt
    18841 gatacggaat cactcatgct atttcttgtt acttcatatt taaaagttgt cgtcgttttg
    18901 ttaccgctcg catctgtaga aacgattgat atagtacttg taccaatgtt tgttggtgta
    18961 ccactaatag tattatttgt actatcaaat gttagtccgg atggcaatcc agtcactgta
    19021 ttcgtcaccg catttccact gttatcttgc gtagcaattt taatcgggga tattggtgaa
    19081 tacacttctg atgattgatc tcctattggt gtcactgttg gtgctgtcgt atccacaaca
    19141 tttattgtaa aagttgtcgt cgatttgtta tttgcttggt cagtagacac aactgtcact
    19201 gttgattgac caatttttgt tggtgtccca atgattgaat tcgttgcact atcgtaactt
    19261 aatccgcttg gtaatcctgt aactgtattt gtcacagtcc cagtaccatt atccgttgta
    19321 gtcaatacaa taggattcat tgttttaccc acttctatgg tttgattgcc tacagttaca
    19381 gttggtgctt ttacatcagt aaaataatat gtcactgatt gtccagcatt cgtcattttt
    19441 acagttttat ttgtatcatt ataagttgac gcatatgaac tatcgacgga cgtgtagtta
    19501 tatcctttag cagtcaatgc agattgctga ttatcgattg tcacgacttg atcaacattt
    19561 cctgaatatg tttttggtgg aataatatct ttacctgttg ttacatcaac gtatctcact
    19621 tgtgtaacag cagactctgt atattcgaat gttccaaatt gtacttgttg taaatttgtc
    19681 gcgccacctg ttgaggctgt cattgataat gaaaagttgg tcgtaccact tttcgcaatc
    19741 caatctgaaa tattacgtgt ccatgtttga cctgcatatt tgacagtcat aacctttgta
    19801 tcaccattat agttaatatc aaaatcttgg aacgtgttat ttgtaggttg aacatttaac
    19861 ttcgcagcat tatcagctgt tgaacttgat gtatacgttg tcgcaacacc ataactatct
    19921 gttgttacaa atgcaccaaa cgcacctcca ccagctacat tagatgggtc agcattcgcc
    19981 tttgcagctg aatttggttt agatgtattg tgatacgtat ccaatttgaa gccaaatgcg
    20041 ttacttaagc caccaatacc tactgcggca ccgtttaacc ctgtttcacc taatacacct
    20101 ggtgaaaagg caaaaccgat accatctcca ccatttccat gcccttcata tttgttacct
    20161 aaatttactt ttccagaaaa atgaaaactc ttattagagt caatacgtgt tcctaatgta
    20221 atagcacctt tttggctgta tgcatcctgt gttaacgtca caataccggt actttgatca
    20281 taggtagcat tacctgacgt tgtcatatat tgttttaagt tatctttatt aactgtaatt
    20341 gtattagcag ttactgcggt tgtcgtcgct gctgacgcaa atgttgacat agctaagcga
    20401 ctgaaagttc gaagttttac tggtgcggtg ctagttgacg ttgtgctagt tttgtttaag
    20461 ttgaccgaag atggcgttgt gctttgtgaa gtgttatttg atgcagtact ttgatttgtt
    20521 gatgtattaa ttggttgttc tgtacttgaa gttgaagcta cagatttagt atcagaactt
    20581 gatgtagtat tctttgagga tgttgattct gatgtagatg tcaatttctc ttgttgattg
    20641 cttgtactat tagttgtcga agtgaccttt tcagactttt cacttgagac tgtgtcacta
    20701 tttgatgttt gtaccgaact actatttttc gttacacttg tggaatcggc tgttgatgtt
    20761 gatgcttcga ttgtcgttga gttttgatta cctactgttt cactttgtgt gtttaattca
    20821 gaagttaatg gtgcatcaga agccgcaaaa gcttgctggt catgcaacat atttaccgtg
    20881 aatgcaccac caataaccgc cgtagttttc agtccgtatc ccgtcatttt tttactaatg
    20941 ctttgattat cttgactcac taaactatga ctaataaatg gtagccccat aattttgaac
    21001 atttctattt ctttaattcc ggattttacc caattttttc cagatttata aagtcttact
    21061 cttgtttttt cgtttgctaa gctgtcatga aatgctttct gtcttttact catgtaataa
    21121 ctccttgtat tatctttaca ttcattagat tataatatat gccactattc aatttaatac
    21181 aactcttttt tgatacaaaa atactcattt tgttaaaat
//
LOCUS       DAKTLH010000031        20879 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.031, whole genome shotgun sequence.
ACCESSION   DAKTLH010000031 DAKTLH010000000
VERSION     DAKTLH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20879
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.031"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            214..882
                     /locus_tag="O0394_001001"
     CDS             214..882
                     /locus_tag="O0394_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCY0499091.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEENPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFFNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            884..3361
                     /locus_tag="O0394_001002"
     CDS             884..3361
                     /locus_tag="O0394_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCY0499092.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSEEVMSDSTKTDTN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            3704..6334
                     /gene="alaS"
                     /locus_tag="O0394_001003"
     CDS             3704..6334
                     /gene="alaS"
                     /locus_tag="O0394_001003"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCY0499093.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAISGGQVAD
                     TGIVYNDNFEIAVSEVIKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            6397..6657
                     /locus_tag="O0394_001004"
     CDS             6397..6657
                     /locus_tag="O0394_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCY0499094.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            6661..7089
                     /gene="ruvX"
                     /locus_tag="O0394_001005"
     CDS             6661..7089
                     /gene="ruvX"
                     /locus_tag="O0394_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCY0499095.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            7104..7412
                     /locus_tag="O0394_001006"
     CDS             7104..7412
                     /locus_tag="O0394_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCY0499096.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            7697..8335
                     /locus_tag="O0394_001007"
     CDS             7697..8335
                     /locus_tag="O0394_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCY0499097.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            8338..9261
                     /locus_tag="O0394_001008"
     CDS             8338..9261
                     /locus_tag="O0394_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCY0499098.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            9273..10541
                     /locus_tag="O0394_001009"
     CDS             9273..10541
                     /locus_tag="O0394_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCY0499099.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            10541..11164
                     /gene="udk"
                     /locus_tag="O0394_001010"
     CDS             10541..11164
                     /gene="udk"
                     /locus_tag="O0394_001010"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCY0499100.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            11192..11668
                     /gene="greA"
                     /locus_tag="O0394_001011"
     CDS             11192..11668
                     /gene="greA"
                     /locus_tag="O0394_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCY0499101.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            11994..12728
                     /gene="pxpB"
                     /locus_tag="O0394_001012"
     CDS             11994..12728
                     /gene="pxpB"
                     /locus_tag="O0394_001012"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCY0499102.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     KFIQIERDISDGNFNVDDWVVIENVN"
     gene            12718..13728
                     /locus_tag="O0394_001013"
     CDS             12718..13728
                     /locus_tag="O0394_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCY0499103.1"
                     /translation="MSIKILQPGLFSTVQDLGRIGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSINASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHSINVPSIAESYATHTRSSIGGFKGRKLLADDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            13730..14179
                     /locus_tag="O0394_001014"
     CDS             13730..14179
                     /locus_tag="O0394_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HCY0499104.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMIGTFFLQDSKELTEPIVNVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            14193..15554
                     /locus_tag="O0394_001015"
     CDS             14193..15554
                     /locus_tag="O0394_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCY0499105.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIENLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKVTLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            15554..16306
                     /gene="pxpA"
                     /locus_tag="O0394_001016"
     CDS             15554..16306
                     /gene="pxpA"
                     /locus_tag="O0394_001016"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCY0499106.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIANVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            16319..17548
                     /locus_tag="O0394_001017"
     CDS             16319..17548
                     /locus_tag="O0394_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HCY0499107.1"
                     /translation="MGRNLKLKKESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQTA
                     VFTSQFFASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISILI
                     AFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMIL
                     GIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAHR
                     ILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVFE
                     HAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVISTM
                     IFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAVL
                     VTILTGIFSFKEVLQLF"
     gene            17837..19057
                     /locus_tag="O0394_001018"
     CDS             17837..19057
                     /locus_tag="O0394_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HCY0499108.1"
                     /translation="MGYRTLKSIFHEHNESKMKEEYIKRFNSLASFNTNINIIPMENG
                     KKVNDLEYPLFFMVTKNLSKKQELISINSRKIDRALNSLPYAAREQYFNDLLIDELQS
                     TNEIENVFSTKQEIAHALNNQASEFLKFRGLVDQYKEIELNKKIKVDNVRDIRAIYDK
                     LVSNEINEQDKLDGELFRKNFVGVHDGSTNKYIHVGLQPETKIVEFIGEMLTFLKYFD
                     APQPFKIMASHYLFEYIHPFYDGNGRVGRFIIAKLLSDYYDNYTALTFSYVINRNKSK
                     YYKAFMTASNHLNCGDLTEFIDTMLELLIAGQERILDELIPKMDATEKLTLYLTSHYK
                     QIDYEFLYLLSMDKLFGNKRNRLTLIDLENILGVGRVKINNTIKKYDNYLVKIKSRPT
                     IYEISDEFLNSIIK"
     gene            19471..20223
                     /locus_tag="O0394_001019"
     CDS             19471..20223
                     /locus_tag="O0394_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HCY0499109.1"
                     /translation="MGEFEVKYLTGFILILLLEGIFTNSASAIEYSDLHHKSKFDSKR
                     LSNAKMSFINPTQLENKNTNDRLLKHDLLFHDMFVNDDWKKDFKVEFENEALSKKFIN
                     KDIDIFAGNYGYGCHGGATNKTQCSYGGVTLSDNNKYDDYKNIPCNLWIDGHQTEIEL
                     TAVKTKKKIVTIQELEVQLRNYLNEKYKLYEQGGDIVKGYVKYYNDDEQNVEYDFYNL
                     NGEYGREVLKMYADNKTINSDKLHLDIYLFKS"
     gene            complement(20425..20694)
                     /locus_tag="O0394_001020"
     CDS             complement(20425..20694)
                     /locus_tag="O0394_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499110.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFTA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
BASE COUNT         7685 a         2742 c         3994 g         6458 t
ORIGIN      
        1 aattatgtat tcttattttt aagtacataa tagtggtggc taatgtggaa gaaccattac
       61 ataataaacc gttaatggtt cttaagcatt tctattccat tcccgctttt tcatgaatga
      121 agatgatatt agattatatt ttattcgttg ttaagtgatt cgagacatac aatttatcaa
      181 gatgttataa ttgatgagaa atgaggttcg taaatgatag atcaacaaac aatttatcaa
      241 tacatacaaa atggaaaaat agaagaagcg ttacaagcat tgttcggaaa tatcgaagaa
      301 aatcctacaa ttattgaaaa ttatattaat gctggtatcg tacttgctga tgcgaatgag
      361 attgaaaagg cagagcgttt tttccaaaaa gctttaacaa tagatccgaa gaatggcgtc
      421 gtatttttta atctagcaaa tgtatattat aatcagcaac gttatcaaga agctattaaa
      481 ttatatcaac aagcattaca aacagagatt gaacaagttg attgtaatta tatgatcggt
      541 atggcgttta atcagttaga atcatttaag ctggcattgc cgtatttaat gactgctgcg
      601 gaactagata aagacaaaga tgcagaagtt caatttcaat atggtcttgt attatgtcaa
      661 ttagaaatgt ttaatgaagc cataactcaa cttaaacatg tattaacgat tgataaaaat
      721 catgttgatg caagatacaa tttgggctta gcgttattta tgaaaaatga agatattgat
      781 gaagcaataa ctcattttaa agaagctgtg actatcgacc ctaaacactt attaagtcag
      841 catgcgctga aaacattcac taaaatgaaa gaggaggagt aacatgtcag accctacact
      901 atttgattat tcaatgatca aaggtactgt tgaagcgata ttgtttcaaa acagtgataa
      961 tttttataca gtgcttaaag tagatactat tgaaacaaat gaagactttg atacaatgcc
     1021 aactgttgta gggtttcttc cgaatattgt tgaaggcgat gtctacacat ttaaagggca
     1081 agttgttgat catccacgtt atggtaaaca attaaaagca gagacttttg aaaaagaaat
     1141 gccacaaacg aaagaagcaa ttataagtta tttatctagt gatttgttta aaggtgtagg
     1201 taaaaagaca gctcaaaata ttgttaatac attaggtgac aatgcgataa atgatatatt
     1261 agatgaccat tcagtgctag aaaaagtttc gggattatct aaaaagaaac agaaacaaat
     1321 tgcagaacaa atttcagcaa atcaagaatc tgaaaaaata atgattcgtt tacatgattt
     1381 agggtttggt cctaaacttt caatggcaat ttatcagttt tatctcggcg atacactgac
     1441 tattttagat cgaaatccat atcaattgat ttatgatatt aaaggtattg gttttaataa
     1501 agcagatcaa cttgctagaa atattggtat cgcttataac gataatgaac gattaaaagc
     1561 tgcgttgctc tatacgttag aagaagaatg tattaaacag ggacatacat atttgcctat
     1621 caatgttgtt atagatttaa cggttgatgt attaaattat caagatgaag aggtcatcga
     1681 acctgaaaaa ttagacgaaa tgttgcaata cttaaatgag gaaaaacgac taattattga
     1741 caatgaacaa gttgcaattc caagtttgta ttattctgaa atcaaaagtg ttcaaaactt
     1801 attcagaata aagacgcata caaataaact tacagaaatt gaacagtcag atttacaaat
     1861 gcatattggt gaaatagaag atgcgaatca agtgaactat gcagcttcac aaaaagaagc
     1921 tttgcaaacg gctatcaatt ctaaggtgat gctattaacc ggtggaccgg gaacaggaaa
     1981 aacgacagta attaaaggta ttgttgaatt atatgctgag attcatggct tatcattaga
     2041 ttatgatgat tatgtcaatg atgattatcc agttgtatta gcagcgccaa caggaagagc
     2101 gtctaagaga ctacaagagt caactggatt agaagcaatg acgattcatc gtttaatagg
     2161 ttggaatcaa gacacaaagc cagaggatat attagaaaat gaaataaacg cacgactcat
     2221 aatcattgat gagatgtcta tggtagatac ttggttgttc caccaatttt taagtgcagt
     2281 tcctttagat gcacaattga tatttgtagg tgatgaggac caattgcctt ctgtaggtcc
     2341 tggtcaagta tttaaagatt taattgaatc gaaagctata ccacgagtaa atctgactga
     2401 agtatatcgt caacaagatg gttcaagcat tatagaatta gcacatcgaa tgaagttggg
     2461 tcagaaaatt gatattacac agcgttttca tgatagaagt tttattaact gtcaggcaaa
     2521 ccaaatacca acagttgttg agaaggtagt tactagtgct gttaataaag ggtatactat
     2581 ggctgatatt caagtgcttg caccaatgta taaaggaaat gccggtatta aacgtttaaa
     2641 tcaagtttta caagatattt taaaccctaa aaagaaagac acacgagaaa ttgagtttgg
     2701 tgatgtagtc tttagaaaag gtgacaaagt attacaactt gttaataggc caaatgataa
     2761 catattcaat ggtgacatag gagttattgt tggtattttt tgggcgaaag aaaatgccct
     2821 taataaagat gtacttgttg tggattttga aggtaatgaa attacattta cgaaacaaga
     2881 tatgatggaa ttaacacatg cttattgtac atcaatccat aaatcacaag gttcagaatt
     2941 tcctattgtt atcatgccta ttgttaaaca gtattttaga atgttacaaa gaccgatatt
     3001 atatactggt ttaactagag cgaaaacgag tttagtttta cttggtgatc cggaagcatt
     3061 tgatattgga ttgaaaacaa atggtcaagc gcgtttaaca caattatgta cattgttgaa
     3121 aaattatttt aattcagaag atgaagaaat gctggaaaac acagcaacta atgatacagg
     3181 agcttcacaa acgacaatag atgatcaagt agaagcgcca atgtcatcga atgatagtga
     3241 agaagttatg tctgattcaa caaaaacaga tacaaatgta ttaacggaag ctacaatgtt
     3301 taaaattgac ccaatgatta atatgggaga aatcacgccg tatgacttta tagaacgttg
     3361 acaacaagcg taaatgtaac taaaatagaa ttaaattcgt ataaagatga gtagttaatg
     3421 atttgtttat cagagatgta ctggttggtg aaaagtacaa caaacattag tgaacgctac
     3481 tttatgcaca actgtatagt cgaattaaag tgataaatgt gttgaattgc atttataact
     3541 agggtggtac cgcgataacg ttcgtccctt ttgaggatgg gcgtttttta ttttttaata
     3601 ataaaaaatg atattgattt atactagttt tgcaaatcga ctatctgtgt catcaatttt
     3661 tatggaatgg taatttaaaa acagtaaata ggatgtgtaa atgatgaaaa agttaaaagc
     3721 gagtgaaatt agacaaaaat atctagattt ctttgttgaa aaaggacata tggttgaacc
     3781 ttctgcacca ttagtgccaa ttgatgatga tacattatta tggattaatt caggtgtagc
     3841 aacattaaag aaatattttg atggacgtga aacacctaaa aagccaagaa ttgtaaactc
     3901 tcaaaaagct attcgtacaa atgatattga aaatgttggt ttcacagcgc gtcaccatac
     3961 attctttgaa atgttaggta acttctcaat tggtgattat tttaaacaag aagcgattga
     4021 atttgcttgg gaatttttaa cgagtgataa atggatgggt atggagccag ataaattgta
     4081 cgttacgatt catccggaag atatggaagc atacaacatt tggcataaag atattgggct
     4141 tgaagaaagt cgtattattc gcattgaagg taacttctgg gatattggtg aagggccttc
     4201 aggaccgaac actgagattt tctatgatcg cggagaagca tatggacaag acgatccggc
     4261 agaagaaatg tatccaggtg gagaaaatga acgctatctt gaagtatgga acttagtatt
     4321 tagtgaattc aatcataata aagatcatag ttacacacca ttacctaata aaaatattga
     4381 tactggcatg gggcttgagc gtatggcctc agtttctcaa aatgtacgta ctaactatga
     4441 aacagattta tttatgccta taatgaatga aatcgaaaaa gtatcaggta aacaatattt
     4501 agtaaacaac gaacaagatg tggcatttaa agtaattgct gaccacattc gtacgattgc
     4561 atttgcaatt tctgatggtg cattacctgc caatgaaggt agagggtatg tattacgtcg
     4621 attgttacgt cgtgccgttc gttttagtca aacgttagga atcaatgagc catttatgta
     4681 caaacttgtt gatattgttg cagacattat ggaaccatat tatccaaatg ttaaggaaaa
     4741 agcagatttc attaagcgtg ttataaagtc tgaagaagaa cgattccatg aaacattaga
     4801 agatggttta gcgattttaa atgaattaat taaaaaagct aaagcgacaa caaatgaaat
     4861 taatgggaaa gatgcattta aattgtatga tacgtatggg ttcccaattg aattaactga
     4921 agaaatagca gtgcaagcag gattgaaagt tgatatgaca acattcgagt cagaaatgca
     4981 acaacaacgt gatcgtgcac gtcaagcacg tcaaaattct caatcaatgc aagttcaaag
     5041 tgaagtattg aaaaatatta catctgcaag tacttttgtt ggttatgata ctgcgacagc
     5101 tcaaacaaca ctaacacact tgatatacaa tggtgaagaa gtttcacaag ttgaagcggg
     5161 tgaaacagta tacttcatgt taacggaaac accattttat gcaatcagtg gtggacaagt
     5221 tgcggataca ggtattgttt ataatgacaa ttttgaaatt gctgttagtg aagtaatcaa
     5281 agcaccaaat ggtcaaaact tgcataaagg agtagtacaa tttggccaag taaatgttgg
     5341 cgctacagtg tctgctgaag tgaaccaaaa tgatcgacgt gacattcaaa agaaccatag
     5401 tgcaacacat ttattacatg cagcgttgaa atcagtactg ggtgatcatg ttaaccaagc
     5461 tggttcacta gtagaagcag atcgtttacg ttttgatttc tctcattttg gtccaatgac
     5521 taatgatgaa attgatcaag ttgaacgctt agtaaatgaa gaaatttgga aaggtattga
     5581 cgttaacatt caagaaatgg atattgcttc agctaaagaa atgggcgcaa tggcattatt
     5641 cggtgaaaaa tatggtgatg ttgtgcgtgt agtaaatatg gcaccatttt caattgaatt
     5701 atgtggtggt attcatgtcc gcaatacttc tgaaattggc ttattcaaaa tagtaagtga
     5761 gtcaggtaca ggagctggtg tgcgtcgtat tgaagcatta acaggtaaag cagctttctt
     5821 atatttagaa gatattcaag agaaatttaa tacgatgaaa tcacagctga aagtgaaatc
     5881 tgatgatcaa gtagtcgata agttaacaca attacaagat gaagaaaaag cattattaaa
     5941 acaattagag caacgtgaca aagaaatcac atcacttaaa atgggtaata ttgaagatca
     6001 agttgaagaa atcaatggct ataaagtatt ggttactgaa gtggatgtac caaatgcgaa
     6061 agcaattcgc tcgacaatgg acgattttaa atctaaacta caagatacaa ttatcattct
     6121 tgcaagtaat gttgatgata aagtatcgat ggttgcaact gtccctaaat ctttaacaaa
     6181 taacgttaaa gccggtgatc ttatcaaaca aatggcacca atcgttggtg gtaaaggtgg
     6241 cggtcgtcca gatatggctc aaggtggcgg tacacaacct gaaaatatct caaaatcatt
     6301 aagctttatt aaagattaca ttaaaaatct ataatttaaa gccataatat tgtatgatga
     6361 tagtgagtta tatttgtaat taaaggagtg tcgcaaatgg aaaactttga taaaacaatg
     6421 aaattcgact atgaagaact tccaacgcaa gatgtaagag atgttttaaa taatgtttat
     6481 cgaacattag atgaacgagg atataatgcc gtaaaccaaa ttgtaggtta tttattatca
     6541 ggtgaccctg cgtatattcc acgccaaaat gaagcaagaa atcaaattcg tcatattgat
     6601 agagatgtta ttatggaaga gcttgtttct tactatttaa aagagcaaaa taaataattt
     6661 atgttacaac ataaaatttt aggactcgat gtcggtagta gaacggtagg aattgcaatt
     6721 agtgatataa tgggttggac ggcacaagga ttagacacac tccgaatcaa tgaagaaaat
     6781 aatgaattag gtattgacca attagtagac attattaaaa aacataatgt gggtacagtc
     6841 gtaataggac tacctaaaaa catgaataat tcaataggat ttcgaggcga agcttcgtta
     6901 acatataaag aaaaattatt agaagcttat ccttctattg agattgtgat gtgggatgaa
     6961 agattaagca caatggctgc tgaacgatca ttattagaag cagatgtttc aagacaaaaa
     7021 agaaaacaag tgattgataa aatggcagcg gtatttattt tacaaggcta tttagattca
     7081 ctacattaaa aggagatttt actatgactg aacataatca tgattcacaa ctagaaatta
     7141 ataacgaaga agaattatta actttattcg atgaagaggg aaatgaagtt ttataccgaa
     7201 aagttttaga attttatcat cctgaattca aaaaagagta tgttatctta gctgaagaag
     7261 gtgctcaatc agatgaagac gatatgattg agcttgtacc aatgatcaat gaaccagatg
     7321 agtcaggtga cggtggtaag ttagtaccaa tcgaaactga tgaagaatgg gacatgattg
     7381 aagaagttgt aaatactgaa atggaagaat aattaaaaag attttactct tgctaacctt
     7441 aaagatgggg aggcaagagt attttttaag tcaagcaatg tataaataat taacgcttat
     7501 aattttattg aaaatatggt aaaatatcta aagttaaatt ttatatgaat gattcgcttc
     7561 tataaataaa atagctttgc gtctaacacg aaatcagtca tttaattggt tggcaaaatt
     7621 ataatcaatt gtgaaggctg atttatttta ttgtcataat catattgact tattttacat
     7681 aaaggaagtt taaaacatgg atgacctaaa taaaaaatat ttaatagatt tacatcaaca
     7741 tcaaaatagt tcaatcgaag ttttgcgtga atttgccgag gtaaatgaag tgccaattgt
     7801 agatcgttta acattagatt taattaagca attaattcgt atgaataatg ttaaaaatat
     7861 tttagaaatt ggtacagcaa tcggctatag ttctatgcaa ttcgcttcta tatctgatga
     7921 cattcatgtc acaacgatag agcgtaatga aacgatgatt caatatgcta aacaaaattt
     7981 agctacttat cattttgaaa accaggttcg aattattgaa ggtaatgctt tagagcaatt
     8041 tgaaaatgta aatgacaaag tttatgatat gatatttatt gatgcagcaa aagcgcaatc
     8101 aaagaaattt tttgaaatat atacaccact tttaaagcac caaggtctcg taattacaga
     8161 taatgtttta tatcacggtt ttgtatcgga tattgggatt gttagatcga gaaatgtaag
     8221 acaaatggtt aaaaaggttc aagattataa tgagtggtta ataaagcaac caggatatac
     8281 aacgaatttt ttaaatatag acgatggatt agcgatttca attaaaggag aatgactatg
     8341 acagaattac ttgtcacacc caagtcatta agccatatgg aaacacttat tgatttaggt
     8401 gcagatgctt ttgtaattgg cgagcaaaag tttggtttaa gacttccagg ggaattcaat
     8461 agacaacaaa tgacagaggc tgtcgcatta gctcacaaaa acgataaaaa agtatatgcg
     8521 gcagtgaatg gattatttca taattatcat ttagatgctg tagaagatta tattaatttt
     8581 ttacatgaaa tacgagttga cagaattatt ttcggggatc cagcagtagt aatgtatgtg
     8641 aaggcgcaag aaaatccaat accacttcat tggaatgcag agacgttagt aactaatcat
     8701 tttcaatgta attactgggg taaaagaggc gcatcaagag ctgtcttagc acgtgagctg
     8761 aatttggatg aaattattaa tataaaagaa aattcaaatg tggaaattga ggtgcaagtt
     8821 caaggaatga cttgtatgtt tcaatcaaag cgtatgctac taggcaatta ttatacgttc
     8881 caagatcgcc aaatgaaaat tgaacgtcgc aatgatgaac aatctttatt actttatgat
     8941 gaagaaagac aaaataatta cccagtttac gaagattaca atggtacgca cattatgtca
     9001 ccaaacgaca tttgtttaat tgaagaatta gcaccatttt ttgaggcggg tatagattca
     9061 tttaaaatcg atggtattct acaaacggaa gaatatatta atgtggtaac ggaacagtat
     9121 cgtcaagcga tagatttgta caatgaagat cctgaaatct atgaagatga gaaatttatg
     9181 ttgatggatc caattgaaga aattcaacct gatcatcgac catttgacga aggtttctta
     9241 tataaacaaa cagtatatta aggaggttaa tcatgaagac aatagaagag attaaatcaa
     9301 ctcctaaaac agttatgaag aaaccagaat tattagcacc tgctggaaac ttagaaaagt
     9361 taaaaatagc agtacattat ggcgctgatg ccgtattttt aggtggtcaa gaatatggat
     9421 tacgttcaaa tgctgataat ttcacgatgg aagaaatagc tgaaggtgtt gaatttgcga
     9481 accgttacgg tgccaaaatt tatgttacga caaatattat tgcacatgat gagaatattg
     9541 aaggtctaga atcatatttg cgtaatttgg aaaagactgg tgcgacaggt atcattgttg
     9601 cagatccttt aattatcgaa acatgtaaag aagttgcgcc aaaacttgaa attcatttat
     9661 ctactcaaca atcactttct aattacaaag ctgtagaata ttggaaagaa gaaggattgg
     9721 atcgtgttgt attagcacgt gagaccggcg cgatggaaat gcgtgaaatg aaggaaaaag
     9781 tagatattga aatcgaagca tttattcatg gtgctatgtg tatcgcctat tcaggtagat
     9841 gtacattaag taatcatatg actgcaaggg attccaacag aggcggttgc tgtcaaagtt
     9901 gccgttggga ttatgaatta ttagaagttg atgataatgg tgaacttgat gttttttata
     9961 atcaaggtga agttacaccg tttgcgatga gtcctaaaga tttaaaatta atcgaatcaa
    10021 ttcctcaaat gatggatatt ggtgtggact cattaaaaat tgaaggacgt atgaagtcaa
    10081 ttcattatat tgcaacagtt gtctcagtat atcgtaaagt cattgatgcg tatgcggcag
    10141 atcctgacaa ctttaagatt aatccggaat ggttaataga gttagataaa tgtgcaaata
    10201 gagacactgc accagcattc tttgagggaa cacctggtta tgaagaacag atgtttggtc
    10261 aacaacaatc taaaaaatca ccatttgatt tttgtggttt ggtattagac tataatgaag
    10321 atacaaaaat tgcgactatt caacaacgaa ataactttaa accaggtcaa gaaatagaat
    10381 tctttggtcc agaaattgaa acatttacac aagtagtaga agcaatttat gatgaagaag
    10441 gtaacagctt agacgcggcg cgccatccat tacaaatcgt tcaaattaaa gtggatcgcc
    10501 cgatatatcc aaacaacatg atgagaaagg aaattggcta atgaaagcta ctacaatcat
    10561 tggcatagct ggtggatctg gctcaggaaa aacaactgta actaacgaaa ttatgaaaaa
    10621 cttagaaggt catagtgtcg ctttacttgc tcaagattac tattataaag atcaaaagca
    10681 cttgactttc gacgagcgcc tagaaaccaa ttatgaccat ccatttgcat tcgataatga
    10741 tttattaatt gaaaatctta aagacttgaa aaatggtaaa gcagtagaag taccgacata
    10801 tgattatgct agtcatacaa gaagtgacat taccattgat tttaaaccta aagatgttat
    10861 tatcgtagaa ggcattttcg ctttagaaaa taaggtatta cgtgatatga tggatgttaa
    10921 aatatatgtt gatacagatg cagacttgag aatattacgc cgtttaacac gagatactaa
    10981 agagcgtggg cgttcaatgg actctgttat caatcaatat ttaagtgttg ttagacctat
    11041 gcatgaccaa tttattgaac cgactaagaa atatgctgat ataattattc ctgaaggtgg
    11101 gagcaataaa gttgcaatag atattatgac aacaaaaatt cagtctttag ttagcaagca
    11161 atagtataat tcagtaaagg aagatgacaa tatggaaaat caaaagcaat atccaatgac
    11221 tcaagaaggt tttgaaaaat tagagcgtga acttgaagaa ttaaaaacag ttaagcgtcc
    11281 tgaagttgta gagaaaatta aagttgcacg ttcatttggt gacttatcag agaactctga
    11341 gtatgatgca gcaaaagatg aacaaggatt catcgaacaa gatattcaaa gaattgagca
    11401 tatgttaaga aatgcattaa tcattgaaga tactggagat aacaacgttg ttaaaattgg
    11461 taaaacagta acgtttgtag aattaccagg tgatgaagag gaaagttatc aaatcgttgg
    11521 ttcagctgaa tcagatgcat ttaatggtaa gatttcaaat gaatcaccaa tggctaaagc
    11581 gttaattggt aaaggtttag atgatgaagt tcgtgttcca ctacctaatg gtggcgaaat
    11641 gaacgtaaaa attgttaata tccaataaat gatcaaatag attatttaat tgcgcgattg
    11701 aagtgtgtta atgatacttc ggtcgtgctt ttttatattc aaaatcaaaa aatcggacag
    11761 atgaaaagta aaaattttcc atcagaccga atgatagcag aacctaaatt agtgtaagag
    11821 ttacaatatt aactactcca ttttaaaaaa gcaaaatatt tccgagaaaa caaattgatt
    11881 atcatttgtt attgtatgcg atttgaatta cttattaaac aacttgtgct tctcaatgat
    11941 acaatgaaaa ggtattcaat actggtttat tgcaatacaa agggggattt aaagtggatg
    12001 taagatttat taacgagcag acaattatga tttattttga aaataaaata tccgaagaga
    12061 catatcgtaa tgtcacagca atggtacggt ggatacgaga aaaagaaata ttagaaattc
    12121 aagatattgt cccttcgtat cgagcagttc taatatattt tgatgaacaa gctataacat
    12181 cttctaaatt aattgaaaat ttagaactta acaaatttaa tgaaaaaaat gttcatgctg
    12241 tgaatcaaac caatcgtatt attaaaatac ctgttcaata tggtggtaca tatggaccag
    12301 atattgaaga agtagcaaag cataatcgaa taactgttga gcaagttatt gaaaaacata
    12361 caagtaaacc ttatttaata tatatgctag gatttatgcc aggatttcca tacttaggcg
    12421 gacttgatga acaactacat acacctagac gtaatcagcc aaggttaaaa attcatgcag
    12481 gttctgtagg tattgcgaat aatcaaacag gactatatcc atcagattct cctggcggat
    12541 ggcaaattat tggacgcaca ccgttaaaag tatttagcag cgagcgtgaa ccaatgtcaa
    12601 tgtacgaagc aggagaatgg atacaatttt atgcaataga tgaacagaaa tttatacaaa
    12661 tcgaaagaga tatatcagat ggcaacttta atgttgatga ctgggtggtg attgaaaatg
    12721 tcaattaaga ttttacaacc aggtctcttt tcaacggtac aagatctagg aagaataggt
    12781 tatgaacata ttggattttc aggcgcaggt gccatggatc aatttagttt taaggttgcg
    12841 cagtcattaa ttaacaatga tggtccagcg attgaatata ctttgattgg tcctaccatt
    12901 caatttaata cgcaaaatac atttgttata accggtggta gtattaatgc ttcgctaaat
    12961 aataaaacta tatcaatgaa ttctgtcata ttagctgaga aaggtgacat tttaaaaata
    13021 ggtgctataa ccaaaggtgc acgcggttat cttacttttg gtcattctat caacgtacct
    13081 tctattgctg aaagttatgc aacacatacc cgaagcagta taggaggatt caaaggtaga
    13141 aagttattag ctgatgatgt aataacagtg aaaataaata atgactttaa agaaaacatt
    13201 ggaaagacga ttcatttaca ggacgattta ttgccagaaa ataatattat acacattctt
    13261 caaggacctc aattcgaggc attttctgaa gaggctagag cgaaaattgt aaatcatcca
    13321 tatttaatta ctgaacaatc agaccgtatg ggctatcgtt tggaaggtga cagcgttgca
    13381 ccaattaatc aagcagatat catttctgaa ccggttgctc ttggtagtgt tcaggtacca
    13441 aatgatggtc aacctattat tctgcttaat gataaacaaa cgataggtgg ttataccaaa
    13501 attgcaactg tatgcaaatt tgatctgcca aagttagcac agatgaaacc acaagataca
    13561 attcaattta aatggatatc attccaagaa gctgtagata agaatcggga acaaatgtct
    13621 ctgtttaatg aaattcttaa gagtcatcaa aagactccaa tttttgatac atctagcttg
    13681 cgacatactt cgaaaaaatt agcaacaatt ttaaaagggg atttgtaaaa tgaatattga
    13741 aaaaatcgaa caaataatca aattagtgaa ggaaaatgat gttaagaaat ttaaatataa
    13801 aaattttgaa gatgaaatag aaattgactt cactgactcg aatcatttgg ctgcacacag
    13861 taatcaatca aatcaaagta tgaacaataa tgatttgaca gcttcaaaag cgaatgataa
    13921 ctccgatgtt tcgacaaatg attatcatga cattaaatca ccaatgatag gtacattctt
    13981 tttacaagat agtaaagaat taactgaacc aattgtgaat gtcggtgaca aagttaacaa
    14041 gggagatatt ataggatatg ttgaagcgat gaaagtatta aacgaggtaa caacagatgt
    14101 tgctggagaa attactgaaa tagtagctga tcatggcaca aatgttgaat acgaccaagt
    14161 tttggtacgt attaagtaaa gggggtaagt aaatgcttcg ttgtttaatt gcgaacagag
    14221 gtgaaattgc tgtaaggatt ataagagctt gcagagaata tgggattgaa actgtagcag
    14281 tttatgcaaa aggggacgag caaagcttgc atgtacattt agcagatcaa gctatatgta
    14341 ttggggaagc taatgcttta gatagttatt taaatattga ccgcatcata tctgctgcac
    14401 aaatcactgg tgctaatgca attcacccag gatatggctt tttatcagaa tcaacaaaat
    14461 ttgctcaaac cgttgaggaa caaggcatag cgtttatagg gcctactaaa aaaacgatgg
    14521 aaatgatggg ggataaaata acagcaagac aaacagttca tcatgcaggt gttcctgtga
    14581 ttccaggatc aaatggtgct gtgaatcatg tgtctgaaat tgagaatctt gccaaagaca
    14641 tcggttatcc agttgttatc aaagctgcca gtggcggtgg tggtaaaggt atacgtattg
    14701 taaagaaagc tgaagattta gaaaaggcat ttaaagaagc taaaagtgaa ggaaaaaaat
    14761 actttgatga tgatcgtgtt tatgttgagg cttttatacc tgttgcaaaa catgttgaag
    14821 ttcaagttat gggagatggg caggataatt atgtacattt aggggaacgt gattgttctg
    14881 tacaacgtaa aaatcaaaaa ttaatcgaag aatctccttg tgcagcatta actgaagaaa
    14941 gacgacaaca aatatgtaac gatgctgtta aagttgcacg tgcagcaaat tatagaagtg
    15001 caggtacgat tgaattttta gtaactgata ccgcacatta ctttattgaa atgaatgctc
    15061 gaatacaagt tgaacatact gttacagaga tgcgagcaga acgtgatttg gttgcagcac
    15121 aactgtattt attggaacat aatcatttac ctttttcaca aagcgatatt caatttaatg
    15181 gtcatgtgat agaagcgaga ataaatgccg aaaatcctga aaagaaattt caaccaacac
    15241 caggtaaagt gacagcgtta catttacctc aaggctttaa tgtgcgagtc gattctttac
    15301 tatatcacgg ttatcaagtt tcaccatatt atgattcttt agtagctaaa gtaattgtaa
    15361 aatctcatga tagagcttca gcaattgata agttgaaagt caccttagat gaaatggtga
    15421 tagatggatt ttctacaact gccgattttt tatatgcagt tttaaattat ccactatatc
    15481 gtgatggaga tgcaaaagat gttgatatta aatttttaga aaaacatcaa attgttaaag
    15541 gggtggaatt ataatgcgag ttgatttgaa ttgtgattta ggcgaagcat ttggaaatta
    15601 ttcctttggt ggtgatcatc aaattattcc gttaattaca agtgcgaatg ttgcttgtgg
    15661 ttttcacgct ggtgatgaaa atgtaatgaa tgaaacggta aaacttgcca aagcacataa
    15721 tgttgcagta ggtgcacatc ctggtttacc tgatttgaaa ggctttggca gacgaaatat
    15781 agatatctct aacgacgaga tttataattt gatgatttat caattaggtg cattacaagg
    15841 gttttgtcgc attcatcaag ttaaaattaa tcatgttaaa ccgcatggtg cattgtatca
    15901 gatgggtgca aaagacagag aaatagcaaa cgttatagca caagctgttt atgactttga
    15961 tccatcacta gtgttagtag gattagcaaa ttcatatcta atttcagaag caaagaatgt
    16021 cggattaatt acagcttctg aagtgtttgc tgatagacga tacgaagatg atgggcagct
    16081 cgttagtaga aaagaaagtg atgctgtgat tactgatact gacgaagcac ttaagcaggt
    16141 tttaaagatg gtgaaggaaa ataaagttat ttcaaaaaac aataaggaag taacgttaca
    16201 agcagataca atttgtgtgc atggtgatgg agaacatgca ttattatttg tttcgaaaat
    16261 tagagaaatt ttaatgaaag aaggcattga tattcaatcc ttataggggg ctaaaaaaat
    16321 ggggagaaat ttaaagctta aaaaagaaag tgactttgaa ttcacaaaaa atcataaaag
    16381 gttattatta ggttctgtat ttttgatggc aacttctgca attgggccag catttttaac
    16441 gcaaacagca gtatttacat cacaattttt tgcaagtttc gcatttgcca tattactgtc
    16501 tatcatcatt gacattggtg cacaaattaa tatatggcgc atattagttg taactggttt
    16561 aagaggtcaa gaaatatcaa ataaagttgt tcctgggctt ggtactgtta tctcaatatt
    16621 gattgcattt ggtggtctag cttttaatat tggtaatatt gctggtgccg gtttaggttt
    16681 aaatgcaatt tttggattag atgtaaaatg gggcgcagct attactgcaa tctttgcaat
    16741 attaatcttt gtaagtaaaa gtggccaaaa aattatggac gttgtttcaa tgattcttgg
    16801 tattgtgatg attttagttg tggcatatgt gatgtttgtt tctaatccac cttatggtga
    16861 tgcttttgtg catacatttg cgccagaaca tccaatgaaa ttagtcttgc ccatcattac
    16921 gttagttggt ggaactgtag gtggttatat tacctttgca ggtgcacatc gtatattaga
    16981 ctctggcatt aaaggtaagc aatatttacc atttgtaaat caatcagcaa ttgctggtat
    17041 tttaactaca ggtattatga gaacgttact attcctagcg gtattaggag ttgttgtaac
    17101 aggtgtgaca ctaagttctg aaaatccacc agcgtcagtt tttgaacacg caattggacc
    17161 aattggaaag aatatttttg gtattgtgtt atttgctgca gctatgtcat cagtaattgg
    17221 ctcagcatac acaagcgcaa catttttaaa aacacttcat aaatcactta acgaaagaag
    17281 taatttaatt gtgattgtgt ttatcgttat ttcaacaatg attttcttat ttattggaaa
    17341 accaatcagc cttttaatta tagcaggcgc gataaatgga tggattttac ctattacgtt
    17401 aggtgcaatt ttgattgcaa gtaaaaagaa atcaattgtt ggtgattata aacacccaaa
    17461 ttggatgttc atctttggta tcgttgcagt acttgttaca atattgacag gtatattctc
    17521 attcaaagaa gtacttcagt tattttaaaa gacatttcga ctaatagcat cactgtaaat
    17581 gttgttagta ttaggcaaga tatattttga tttccggtga ttcatttata aagcataatt
    17641 agatatattt taagggagag tggtgcatga caaatgtact gctctttttg ttgtgatgaa
    17701 aaatcttttt ctattgattg agaaaatagt tatttatata gtttttaaag aaaaaaagtg
    17761 ataatattta gctaatctaa tgaaaattga tttattggac aaaaaataca ttttaaatga
    17821 gtagaggagg ctgcaaatgg gttacagaac tttaaaaagt atttttcatg aacacaatga
    17881 aagtaaaatg aaggaagagt atattaaaag atttaattct ttagcttctt tcaatactaa
    17941 tattaatatc atacctatgg aaaatggaaa aaaagttaat gatttggaat atcctctatt
    18001 ctttatggtg actaaaaatc tatcaaaaaa acaagaatta atatcaatta atagtagaaa
    18061 aattgataga gcacttaatt ctttaccata tgcagctaga gaacaatatt ttaatgattt
    18121 attaatcgat gaattacaaa gtactaatga aattgaaaat gtatttagta ctaaacaaga
    18181 gattgcacat gcgttaaata accaagcatc agaatttctt aagttcagag gcctcgtgga
    18241 tcaatataaa gagatagaac ttaataaaaa aattaaagtt gataatgtaa gagacattag
    18301 agcgatttat gataaattag tttcaaatga aattaacgaa caagataagt tagatggaga
    18361 gctatttcgt aaaaattttg tcggtgtgca tgatgggtca acgaataaat atatacatgt
    18421 tgggttacaa cctgaaacca aaattgttga atttataggt gaaatgctaa catttttaaa
    18481 atattttgat gcgcctcagc cgttcaaaat catggctagt cattatctgt ttgaatatat
    18541 acatcctttt tatgatggca atggtagagt tggaagattt ataattgcca agctcttgag
    18601 tgattattat gataattata ctgctttgac attttcatat gttataaata gaaataaatc
    18661 gaaatattac aaggcgttta tgactgcttc aaatcatttg aattgtggtg atttgacgga
    18721 atttattgac acaatgcttg agctattgat agcaggacaa gaaagaatac ttgatgagtt
    18781 aataccaaaa atggatgcga ctgaaaaatt aacgctttat ttaactagtc attataaaca
    18841 gatagattat gaatttttgt atctcctttc aatggataaa ttgtttggaa ataaaagaaa
    18901 tagattaaca ttaattgatt tagaaaacat attgggagta ggaagagtaa aaattaataa
    18961 tacaattaaa aaatatgata actacttagt taaaattaaa agtagaccaa caatttatga
    19021 gataagtgat gaatttctaa attcgattat aaaataaata taatatgtga tcagctatac
    19081 ttatagtatt tgtaagatcg cgtaattgct tcattgaaaa aatatatttg ttgagagtgg
    19141 tgcatgacaa atgtactgct ctttttgttg tgtatcagta ttgtgatgtt ttaatgagaa
    19201 tattatatga atcattatga aatttaataa aaataaaaga aatgattatc attttttctt
    19261 atatactgtt aaacggtttg gaatttttag gtatacactg tattggttga tataactcaa
    19321 ctaataattg cgaacagagt atttcaaatt gaaaagtatt atgagcgtga tacataatca
    19381 aaattgtagg ctcaagaacc actacataat aaaccataag cggttcttta tcatttatgt
    19441 ctcgctctca aatgtaaatt aataattgtt ttgggggagt ttgaagttaa atatttaaca
    19501 ggatttattt taatattatt gttagaagga atttttacaa attcagcgag tgcaatcgaa
    19561 tattcagact tacatcataa aagtaagttt gattcaaagc gtctaagtaa tgctaagatg
    19621 tcattcatca atccaactca gcttgaaaat aaaaacacaa acgatagact gttgaagcat
    19681 gatttgttat ttcatgacat gttcgtaaat gatgattgga aaaaggattt taaagttgaa
    19741 tttgaaaatg aggcactttc aaagaaattt ataaataagg atatcgatat atttgctgga
    19801 aattatggat acggatgtca tgggggagca accaataaaa cgcaatgtag ttatggtggt
    19861 gttactttaa gtgacaataa taaatacgat gattataaga atataccttg taatttatgg
    19921 attgacggac atcaaacaga aatagaacta actgcagtaa aaacgaaaaa gaaaattgtt
    19981 actattcaag aattagaggt tcaattaaga aattatttga atgagaagta taagttgtac
    20041 gaacaaggtg gcgacattgt taaagggtat gttaaatatt ataatgatga tgaacaaaat
    20101 gtagaatatg atttttataa tttaaatggt gagtatggtc gtgaggtatt aaaaatgtat
    20161 gctgataata aaactatcaa tagtgacaaa ttgcatttag atatctattt attcaaatca
    20221 taatttaatt aagttaatca ttaataatct ttgacagtga aatataaagt acctgtcatt
    20281 atgttgttac aaataaaaac agcagtaagg atgctaacac ttagaaaagt cttactgctg
    20341 ttttctatta gaattatctt taatgcacaa aggaattcga tacgaaacaa atatgattaa
    20401 ctgtgtaatt acaaaataaa actactatag taaaaagaag ataaagacga tgataataaa
    20461 tgcatcgaat ataattttct tgatgttttt atcaacaaca tcaggtaagg acataagtat
    20521 aaatcctaga attagagtgt agcgacatat gttcataatt gttgctgtaa actcgatatt
    20581 aaatgaaaac tttaaaaata tacttataaa taaaatgaca aacgacgcaa ttgcaataaa
    20641 actgccgtat gtagctatct ttgttacgag actattttga ttattttcag ccattgtaaa
    20701 atcctcttat ttcatcatta ttattttaaa catatcattt tcctgttatt taataaagtt
    20761 tcttgacacg attggttgat gtaattgata tgattagttt ttgaaaaata gacaaattag
    20821 aagcatcgaa ttcactgtta tgagttcaaa aataattatt tatgatacca agttattga
//
LOCUS       DAKTLH010000032        20789 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.032, whole genome shotgun sequence.
ACCESSION   DAKTLH010000032 DAKTLH010000000
VERSION     DAKTLH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20789
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.032"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(53..127)
                     /locus_tag="O0394_001021"
     tRNA            complement(53..127)
                     /locus_tag="O0394_001021"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(92..94),aa:Glu,seq:ttc)
     gene            complement(557..1840)
                     /locus_tag="O0394_001022"
     CDS             complement(557..1840)
                     /locus_tag="O0394_001022"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCY0499111.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTNVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            complement(2118..3518)
                     /gene="dnaB"
                     /locus_tag="O0394_001023"
     CDS             complement(2118..3518)
                     /gene="dnaB"
                     /locus_tag="O0394_001023"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCY0499112.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            complement(3550..3996)
                     /gene="rplI"
                     /locus_tag="O0394_001024"
     CDS             complement(3550..3996)
                     /gene="rplI"
                     /locus_tag="O0394_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCY0499113.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            complement(3993..5960)
                     /gene="gdpP"
                     /locus_tag="O0394_001025"
     CDS             complement(3993..5960)
                     /gene="gdpP"
                     /locus_tag="O0394_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HCY0499114.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIILFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            complement(5975..6913)
                     /locus_tag="O0394_001026"
     CDS             complement(5975..6913)
                     /locus_tag="O0394_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HCY0499115.1"
                     /translation="MGVAYVFSKIQPKATIIATIALVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LVIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            complement(7204..8172)
                     /locus_tag="O0394_001027"
     CDS             complement(7204..8172)
                     /locus_tag="O0394_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCY0499116.1"
                     /translation="MTNYTVDTLKLGEFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHVFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     regulatory      complement(8232..8330)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(8535..8864)
                     /locus_tag="O0394_001028"
     CDS             complement(8535..8864)
                     /locus_tag="O0394_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCY0499117.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AALRFFF"
     gene            complement(8861..9556)
                     /locus_tag="O0394_001029"
     CDS             complement(8861..9556)
                     /locus_tag="O0394_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCY0499118.1"
                     /translation="MTTHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIVL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     TLGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMEQ"
     gene            complement(10206..11492)
                     /gene="serS"
                     /locus_tag="O0394_001030"
     CDS             complement(10206..11492)
                     /gene="serS"
                     /locus_tag="O0394_001030"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCY0499119.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     misc_binding    complement(11582..11798)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11871..13385)
                     /gene="hutH"
                     /locus_tag="O0394_001031"
     CDS             complement(11871..13385)
                     /gene="hutH"
                     /locus_tag="O0394_001031"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HCY0499120.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQSEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYDEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
     gene            13711..14523
                     /locus_tag="O0394_001032"
     CDS             13711..14523
                     /locus_tag="O0394_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCY0499121.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
     gene            complement(14610..17273)
                     /gene="gyrA"
                     /locus_tag="O0394_001033"
     CDS             complement(14610..17273)
                     /gene="gyrA"
                     /locus_tag="O0394_001033"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCY0499122.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDLSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFENLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEE"
     gene            complement(17310..19244)
                     /gene="gyrB"
                     /locus_tag="O0394_001034"
     CDS             complement(17310..19244)
                     /gene="gyrB"
                     /locus_tag="O0394_001034"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCY0499123.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            complement(19254..20366)
                     /gene="recF"
                     /locus_tag="O0394_001035"
     CDS             complement(19254..20366)
                     /gene="recF"
                     /locus_tag="O0394_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCY0499124.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            complement(20363..20608)
                     /gene="yaaA"
                     /locus_tag="O0394_001036"
     CDS             complement(20363..20608)
                     /gene="yaaA"
                     /locus_tag="O0394_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCY0499125.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
BASE COUNT         6287 a         4157 c         3146 g         7199 t
ORIGIN      
        1 ctcctgcgtg acaggcaggc gtgttaaccg ctacactacg agacctaaaa aatggtgact
       61 cctacgggac tcgaacccgt gttaccgccg tgaaagggcg gtgtcttaac cgcttgacca
      121 aggagccgtt cttaagcaca agatagatta tagcacagcc aatcttgcca tgacaagata
      181 ttttttacat aattttgcta ttattttttg aagaatgtta taagtgaaaa attgcatttt
      241 attggcagtc tattcattat aataattacg ctatttttga aatttttcta ttttaggtgc
      301 tttcatttac acttacgtct actctatttt aatagctatt ttcgacatca gacttcttac
      361 attcaagtat ttcatcttaa ttttcgttaa atatcatcta atcttctaaa ttatgactag
      421 caaatatcgc ctttacgtta gtatttttta tctaaataat cgaatcgata cagaagcaag
      481 cataacaaaa gcctcctacc aataaagata gaaggctcat gtatttataa tcattgtgta
      541 tgacttatat aaagttctac cacaattctt ttaataggtt tgtttgttct ctatctggac
      601 caactgagaa gatagaaatt tgtacattac ataattctga aatacgctct aaatatttac
      661 gtgcattttc aggtaattct tctaaagtac gcacatttgt tacgtcttct gtccaacctg
      721 gtaactcttc aaagattggt ttacaacgtt ttaattgatc taagtttgct gggtactcag
      781 taatttcttt accgtctaat tcataagctg tacagatttt cactgtgtct aggcctgtta
      841 aaacatcgat tgagttaata gataaatctg taataccact tacacgacga gagtgacgta
      901 atacaactga atcaaaccaa cctacacgac gtggacgtcc tgttgttgta ccgtattcac
      961 gaccaacctc tctaatatga tgtccatctt catcgaataa ttcagtaggg aatggaccat
     1021 caccaacacg tgatgtataa gctttacata caccaattac ctttgaaacg aatgtaggac
     1081 ctacacctgt accaacagta acgttacctg caattggatt acttgatgta acgaatggat
     1141 atgtaccatg gtcgatatct aacattacac cttgcgcacc ttcgaaaagt accttttcat
     1201 ctgctacaaa tgcatcgtct aagatttttg atgtgtctgt tacaaattct tttagacgtt
     1261 gacctgctgc ataatattct tcaaagatat catcaaatga tggacatgtt tcgttaaaca
     1321 tacctttgaa atatgcttgt ttatattcaa tgtttgattt taataatctt tcgaatgttt
     1381 ctttttcaag taaatctgcc atacgaatac cgatacgttg aactttgtct acatatgctg
     1441 gaccgatacc ttttttagtt gtaccaatct tattgtcacc acgtaaacgt tcttcatatt
     1501 catcttgtgc taagtgatat ggtaaaatca cttgcgcacg attagatata cgtaaattac
     1561 ttgtaggaat gccacgttca tttaatccgt ctaattcttt caatagtgca actggatcaa
     1621 caacgactcc gttaccgatt accgctaatt tgtctttgta aaagatacca gatggtacta
     1681 aatgtaattt atatgtttct ccgccaaatt gaatggtatg gcctgcatta ttaccacctg
     1741 aaaaacgcgc gataacatct gactgttctg ccaagaaatc cgttattttt ccttttcctt
     1801 cgtctcccca ttgtgtccca actactacga ttgatgacat gtgagcacct ccaagttttt
     1861 ctcatttaac catttgatat tctaccaata tcgagaccat aatgcaaata aaaatcgaac
     1921 attgattttc ggccttttta aaagaaaaat cattttaaca aatcgttttc aacgtcctaa
     1981 aaacccttta acagaggcgt tgacagtact aatttatcac tttcaataca caaacttaac
     2041 atatatcaac ccgttcagaa caaaataaaa accgtacaat tttatcatct taatgattat
     2101 tgtacggaaa aactttttta catcatatct gcatgtgcat aatcgatatc ggtaaattta
     2161 ttatattgtt tcataaaatg taacttaact gtgcctgttg gaccgttacg ttgcttagca
     2221 atgataattt caatttcacc gttttcatca ttcgtttgtg gctcgaaacc accatcatcg
     2281 tcatcatctt catcgccgcc acggttatag taatcatcac ggtataagaa tgcaacgata
     2341 tcggcatctt gctcaatcga accagattca cgaatatcac tcatcattgg acgtttatct
     2401 tgtcgttgtt caacaccacg agataactga cttaatgcga taactggaca ttctaattca
     2461 cgggctaatg cttttaatgt acgagagatt tcagaaactt cctgttgtct gttatcggac
     2521 gcacgtgaac cactaccttg aatcaactgt aagtagtcaa tcacaatcat gtctaagcca
     2581 tgttcttgct ttaatcgacg acatttagaa cgtaaatcat taattcgaat acccggtgta
     2641 tcatcaataa aaatcttcgt acgtgataat ttacctaccg ctatagtaaa acgactccaa
     2701 tcttcctcag tcatagtacc cgttcttaag cggtttgagt caacatttcc agaactacaa
     2761 atcatacgtg tggctaactg atcagcaccc atctctagcg agaaaatacc aactgtatac
     2821 atatcttcat gcgttgcaac tttttgtgca atattaagtg cgaacgcagt cttacctaca
     2881 gatggacgcg ctgcaaggat aattaaatca tttcggttga accctgctgt catttggtct
     2941 aaatctcgat atcctgtagg tatacctggt gtttgaccac tattttgatc aagctcttca
     3001 gctgtttcat acacttgtcc taagacgtct cgaatgtctt taaagccatc gctttcacga
     3061 gaagatgata gctctaaaat tcgacgttct gcatcactta aaatcgcatc tagttcaagt
     3121 tcatcattat atccatcatt ggcaatacta tctgcagttt gaatcaatct acgttttaat
     3181 gcatgcttag aaacgatatc agtataatac tgaacatttc gcgtcgttgg tacatttgta
     3241 gataactctg caagatattg cgggccaccc gcttcattca acgtaccttc cgtcgataat
     3301 tgatccatca atgttacaac atcaatttct ttattatctt catttaagtg catcattgca
     3361 cggaaaatat gttgatgggc acccctataa aacgactcag gaagcaaaac ttcctgagta
     3421 gtattaatca attctggatc tataataatt gaacctaaga cagactgttc agcttcattg
     3481 ttatgcggca tttgattttg ctcatacatt ctatccatga atggttacac ctcttatttc
     3541 aatccaactt tattgttcaa ctgtgtgtac gcgaattgta ccttcaactt ctttatctaa
     3601 tttaacaggt acattcgtat atcctaggga atgaattcca tttggtaaat ccattttacg
     3661 tttatcaatt ttaatatcat gttgtgcttt tagtgcttcg gcaatttgtt ttgtacttac
     3721 tgacccaaac aatttaccac cttcaccagt ttttgctgat acttcaactt caatgtttga
     3781 taacgtttct tttaatgctt tagcatcttc aatttcttgt tggcgttctt gttttgcacg
     3841 ttttttctgt aactctaatt gtttaaggtt acctggtgtt gcttctacag cataattctt
     3901 tttcaataag aagttatttg cataacctac tggtacttct ttaacttcac cttttttacc
     3961 tttaccttta acatcttgtg taaaaattac tttcatgcat cttcactcct acttaattgt
     4021 tctgtaattg cttgttgtaa ttgtgctatc gcctcttcga ctgtcacacc tttaagttgt
     4081 gttgccgcat tggttaaatg tccaccgcca ccaagtgctt ccattgttaa ctggacattt
     4141 actgaaccga gtgaacgcgc agatatacca atcagattat cttcacgtct cgcaacaaca
     4201 tatgatgctt caataccttc taaacttaac agttcatctg ctgcttgtgc aactgttact
     4261 ggatgataaa ttttatcgtc tgaaccatgc gcaatggcta tgccattatc ttcaactttt
     4321 acagttcgaa ttaattcaga tcgattaatg taagtatcca catcatcttt taagaaatgt
     4381 tgcgttaaaa tcgtatctgc accatgtgca cgtaaataac tcgctgcatc gaatgttctt
     4441 gatcctgttc gtaatgtaaa gtttcttgta tctacaataa tacctgcata catcactgtt
     4501 gattcaagac gtgttaaacg ttgttctgtt ggttgatatt ccagtaactc tgttaccaat
     4561 tcagctgtcg aacttgcgta tggttccata tatatcaaca atggattaga gatgaagctt
     4621 tcaccacgtc tatgatgatc gataacaact ttacggtttg ctttatttaa gacattttca
     4681 tctaaaacca gttccggttt atgcgtatca acaatcacta cggttgtctt agatgtcatc
     4741 atatcccaag catcatctga tgtaataaat cgctctctta actctggctt tttatctatt
     4801 tcgttcatca cgcgtcgtaa tgttggatca atgtcagtct catttaatac gatgtatgct
     4861 tctaaattat tcatcattgc aaatctagac acaccgattg ctgcaccaat tgcatctaag
     4921 tcaggacgtt tatgtcccat gataatgact ttgtcaccct ctgcaaggat atcttttaac
     4981 gcatgtgaga tcacacgtgc tcttacacga gtacgtttct ccatcgggtc agtcttaccg
     5041 ccatagaaac gcacattacc attaatactt ttaattgcaa cttggtcgcc accgcgtcct
     5101 aatgctaagt ctaggcctga ttgtgataat tcacctaagt cgattaaatt ttcagtacct
     5161 tcaccaacac cgatacttaa tgttaattgg gcacgataac caacactttt ttcacgtaat
     5221 tgactcaaga tatcaaattt agattcttct aagtcagcta atattttttg atttaaatag
     5281 gctacgaatt gatcggaact gtatcttttg aaaaatatat tatactcagt tgcccatcga
     5341 ctaatgacac gcgttaccat tgagttgatt tccgaacgct gcgtatcatt catattttgc
     5401 gtaatctcat cgtagttatc taaaaataat gtcgcaatga ttggtttaga attttcatat
     5461 agttcatttg tttgtacttg ttcagttata tcaaagaaat agaggcagtg atcattctca
     5521 gaataacgta cttggaaatg atactgatta tattctattt caacggattt cactctatct
     5581 aattgcttta aaatgtttgg aaatacttca tttacagatt cagaaatgac attcgcttcc
     5641 atatgatctg tcataaattg gttaacccat tcgatgtgat cattttcatc taaaacaatg
     5701 ataccaattg gtaaatgttt gattgcttta ttatttgttg ttgaaatttg agcactcaaa
     5761 ccatctacat aactatccat tttcattaaa gcttgtctga ataaaatgat gctaacaata
     5821 atcatcacga caagaacgat agatgcaatt agtgctataa gactattaaa gataaaccat
     5881 acacccatta aaacaattgc tgtgatgatc atgatgacaa atggtattag taaagctttc
     5941 ttagtggact gccgattcat tattccacct ctattcactt tttagaatta tttttcatga
     6001 ttcgcttcaa attcaaactt aaatcgataa caccaagtag tcctacaata tgtgtcgtag
     6061 gtgtcagtat tgtaccgata accaatagta aaatcgttac tgcattcggc aaacctttcg
     6121 ctttaccaaa gaaatgaata acacttaaac cttgaatata cattactaat gataacacaa
     6181 gttggaagtt taaaagaatg ctctggaaca cactcggttg acctgtaaat aataaacata
     6241 tgataacaat aatgtatatc cataataaaa taccgctcat ttgccacgcg aaaagtggct
     6301 taaatacagg tgtagcgatt ttaaattttc gtaaaatcgg aaatgtaacg attaagttaa
     6361 ttaagacgat taaaaatgta atgataatga tgaaacctgg taattgaacg gtcgcttgtc
     6421 taaacccttc ttctaatatt tgggtcatat tcgcatcggc accgctcatc gtaatcgctt
     6481 catgtaatgt ttgcttgaaa ggttttacta tgctcgctga tggtggaatc cttccgaatg
     6541 tttgtagtaa cataaaagcg attaatgaaa ttaagctcat cgctactgtt gttacgtata
     6601 atattctttc tttagacgtt ctttctttga gcaattgacc aataattaaa cttgcaatta
     6661 agactaatat gatggcactt aaaacgaaag tattacctaa aacagttgtt ataattactg
     6721 taataagtgc actaatcccg aaagattgta ttgatttatt ccataaaacg atacctggta
     6781 tggttgcaaa taatgcaaat attagtccta aaccaggcac tagatataaa gctaaagcga
     6841 caaataccaa cgcaatcgtt gcaattattg ttgctttagg ttgtattttt gaaaacacat
     6901 aagccactcc catattttta actatagcta ttattttaac ctctttaatg aaaattaaca
     6961 atttatagat tgtatgcttc tatttcattt aattgaataa taactttcat gttttataag
     7021 taattaacat actcatttga atcgcttttg tgtgctttca ttttcaacat gattatttaa
     7081 tcccactaca tagcaatcaa gcttgattta gatttacaat acatttccac tctcatgtac
     7141 tctagatgtt tttgaatatg ataactgtga tttagtggct tcattctttg aaaatatata
     7201 ttattactta cgcttaaaat gctttaaatt taagaaatga tataagttag gtgcccaggt
     7261 actaaagttt agtaggaatc catcatgccc aacattatca ggcacgaaga aatgacgatg
     7321 atatttaaaa cgttcaccta atgcacgaac ttgatcatcc ggatatagca aatcatctat
     7381 gaaccccatc gttaacactt ttgtttctaa atttttaaaa acatgcgtta cgtctgtgcg
     7441 acctcggtca atgttgtgac tatccaatac atctagcagt gtcagataac aattcaaatc
     7501 aaaatgttct ttaaatttat taccttgatg ttgttggtat gcgactactt catccggcgt
     7561 gaaacgttca tcataacttt ttgatgatcg atatgtcaaa aaacctaatt ggcgtgcaat
     7621 acttagacct tccttaccac caagatgaat ggcttgcctt gcaatttcat tgaaagctct
     7681 actataagat gatgttcgac ttgttgcagc aaggataatg gctttatcta cttcaaactg
     7741 ttgattgtag agtagttcca ttgcttgcat acctccaaga cttcccccta ttaaaatatt
     7801 aatcttatca taaccaaggg cttgtatacc tcgttcattc gctctgacta tatctcttaa
     7861 tgttaatttt ttaggaaaat gagggtcgtt taaaggtgaa cttgaaccga aaggactacc
     7921 aataacatca aatgttaaaa attgataatc gtgaatgggt atatatcccc catcaataat
     7981 ttctcgccac caacccggat aatcatctgt tccatatgtt aaatgattgc cagttaatgc
     8041 atgacaaact acaactaatg gttgtccatg ataaccgaca tgctcatatc tcaaacgcaa
     8101 gttatctatg acttccccag attctgtaat aaattcccct aattttaaag tatctactgt
     8161 gtaatttgtc attgttcttt cctccttaaa caaaaaaact tctcacccta ttgaaaagta
     8221 agaagtcttt atacttatca ttcgagtaac tcgttggttt tagcaccgtg ctataaagtc
     8281 ggttgctgaa gtatcacagg gccaagtccc tcaacttctc acaataagaa atatgaaatt
     8341 gttatgtgtt agttgagatt cagtgatgaa ttacttttat catttaaaat gttgttatca
     8401 ttgtcatgcg ttagccaaat cgcttacgta tacacgattc ccaatcttaa catagacgat
     8461 ttgtatatca gaattttctg attactaaca gtttacctaa gtttaaatat ctgttcaatg
     8521 attttcagtt atttttaaaa gaaaaatcgt aatgctgcca tgataacaat cccactaata
     8581 attgtaatag ttaaactacg cgtgattata gataaaataa ccgtcggaat gagcgcgata
     8641 atgtaaggga tgtttaatgt atacccctca ccatgaggcg tctgttgaat aatgctgtca
     8701 atgacaagtg ccgtaaatag tgtgattggg ataaatgata gccatcgaac cacgacatca
     8761 ggcaattgca cttttgaaat catgataaaa ggtataattc gaattaatag cgttacgata
     8821 ccacacaata aaataagtat taacatgttc atatgagtta tcattgttcc atcatcactc
     8881 ctaacgctgc tgaaattgtg gctgcaatta atattgctag atatgaaggc ataaacatac
     8941 ttagcgataa catcattact atgacggcaa taatgagtac tatgtaaatt cttaatcgcg
     9001 atttagtaat tgattcaaat tgcgcaatgg ccaaaaagat aaacatagcc gtgatagcaa
     9061 aatctaaccc tagcgtttgc ggatttgaga tatattcgcc aaataaagcc ccagctacac
     9121 atgaaattgc ccaaaataaa tatgctgtga tgttaagacc atgcatccaa cgatcattga
     9181 tagcttctcc ttttaaataa ggtgtaatgg cgacgccaaa cgtttcgtca gttactaatg
     9241 aacctaatcc aacacggttc caaaacccat atgtcttgaa gtttggtgca agcgacatac
     9301 ttaaaaggaa cattcttgaa tttacgataa atacagttag tacaatcgct gatataggtg
     9361 tacctgctat aaacaacgcg cacataataa attgcgcagc accggcatat ataacaagac
     9421 ataacaagac aatttctaaa atactaaagt tttgagacga agccacaata ccaaatgaaa
     9481 taccaacacc ggcataaccc aataatgttg ggatacactc ttgcacgcct tgtctaaaac
     9541 ttaaatgtgt tgtcatctca attacctcct ttgccacttt atatttatat aattacgaaa
     9601 aaataatata cctagtctaa tacagttcta taccctaagc aataacatta gacatcagtt
     9661 tgtctgaggt tagacatacc ggagtcttta gtcagcttca tattaacttt ttatttttga
     9721 gaattttcaa ttttttattt aagactacct ccatattttc tatggatttg tagttgtttt
     9781 taagtatcaa ttttataaat ttttatatct gatgatgagt ctgggatatt gattcatgta
     9841 ccactccctt ataatcatcc cctcccccta ccctactcca tcgatataac tcatactaca
     9901 tatcaacgaa atcagtattt tatcgcttcc tttcctatat tggtgatgct caaacttgtt
     9961 acgttttaga ttgttttagt tcatcataat tatcccgtat tgttgctata atgaaatgcg
    10021 ttcaccccat taaaccacaa acttaattta ttgttgttat gtgcattggc tcactattat
    10081 acttttacag cacaaaaaaa gtggcgacag cttcgtcacc actttttaaa atattattta
    10141 aagtatcttg cccttgcttt aagtatacgt agatatatac tttttaaagc ttgtagctaa
    10201 agcctttatt taactggttt tgaaatttgt gttttaccac ccataaatgg tactaatgct
    10261 tctggaattg ttactgttcc atcttcattt tggtaatttt caacaatagc agcaaatgta
    10321 cgtccaactg ctaaaccact accatttaat gtatgtgcta attctggttt agctgctttg
    10381 tcacgcttga agcggatgtt agcacgacgc gcttggaaat ccgtacagtt tgagcatgaa
    10441 ctaatttctt tataatcatt gtagcttggt aaccaaactt ctaaatcata tgttttgctt
    10501 gcactaaatc caatatcacc tgtacataaa ataacacgac ggtatggtaa acctaactct
    10561 tctagaattg cttctgcgtt tgttgtcatt tcttctaaag cattccatga atcttcaggt
    10621 tgttcaaaac gtaccatttc cactttatcg aattgatgta aacgaattaa tcctcttgta
    10681 tctctacctg ctgatcctgc ttcactacgg aaacatgcag attgaccagt gaatttttca
    10741 ggaagtacac ctggttgaat aatttcatta cggtagaaat tcgttaatgg tacttcagca
    10801 gttggaattg tatataatcc ttctttttct actttaaata aatcttcttc aaatttaggt
    10861 aattgacctg taccatacat tgtatctgcg ttcacaagct gtggtaccat catttctgta
    10921 taaccatgtt gtgttgtatg ttttgtaatc atatagttca ttaaagcacg ctctaattgc
    10981 gcaccttcat ttgttaaata tacaaaacgc gcacctgaaa cttttgctgc acgatcaaaa
    11041 tcagccattt tcaattcttc tacaatatcc caatgtgctt tgggttcaaa tgaaaactca
    11101 cgtggtgtac cccacttttt aacttcaacg ttatcttcat cagattcacc ttgaggtaca
    11161 tcatcactta ttaaatttgg aatacgacaa aggatacctg tcattttatt atcaatttca
    11221 tttaattgac tatctttttc tttaatatcg tcacctaatg tgcgcatttc agcaatcaca
    11281 tcatcagcat tttctttatt acgttttttt aatgcgattt cttcgcttac tttattacga
    11341 cgtgctttca tttcttctgt tgcactaatt aatttacgtc gttgctcatc caattctaaa
    11401 atttcatcta caacttttgg atcatctcca cgtaattcaa ttttgctctt aactgtgtca
    11461 ggctcatttc tgaataatct aatgtctaac attaaccttc atcctttccc aaataattat
    11521 catttattat ggaatgacgt acgtctttat tttttagaaa ataaaaaaag accacatccc
    11581 tacaagggac gtggtctacg cgttgccacc ctatttaaca atttaagtta taaagataca
    11641 ctaaacctaa attgcacttc actaaaataa cggttatcac cgattgttct tttaaattaa
    11701 gtaggtagat tcatatatat gttgattctt gttcacacta accacaagct ctctgatatc
    11761 gaacactata tattacttgt cctacgaaca atgtcttatt aagttatttt taatatagca
    11821 aactatattt gctttttcaa gtaacgattt caaacatcac tcatgtcgat ttagtgacat
    11881 gcagtcgttt gataaattga ttgctttaaa tactgtgcaa ccgcttcaat atctttatga
    11941 aattgacgat catgtgtaat ggatggcacg atacttcgaa actcatcata cttgcgacgt
    12001 gtttttggtg ataatccttc aacacctttt aactctgctg cttgtaatgc aataacacat
    12061 tcgattgcca gcacacgtct tgcattttca ataatttgat aaccatgtct agcagctgta
    12121 gttcccatag atacgtgatc ttcttggttc gcagatgaag tgatagaatc aacactcgct
    12181 ggatgcgcta aagttttatt ttcagaaacg agacttgcag cagcatattg cataatcatc
    12241 gcgccacttt gcaatcctgg ctctggacta agaaatgctg gtaaatcacc atttaattga
    12301 ggatttacta gtcgctctag acgacgttcc gatacgtttg ctaattcact tacacctaat
    12361 ttaagatgat ctaatgcaaa agcaataggt tgtccatgga agttaccacc tgaaataaca
    12421 aacgtttcat ttgcttcctc aaatataagt ggattatcat tagccgcatt catttcaaat
    12481 tctaattgct gtttaacata attgaatact tgaaaactcg cgccatggat ttgtggtata
    12541 caacgcaacg tatatgcatc ttgtacacgt atttctgatt gtcgcgtcgt taatgttgat
    12601 ccttctaacc aatcacgcat acgcgctgcc acattaatct gttcttgaaa attacgaact
    12661 gcgtgcacat catgtcgata tgcatctata atgccattaa gagactgatg cgttaatgca
    12721 gcaatccatt cagattggta acctaaatct tctgcttcta tataactaat gacaccttga
    12781 gctgtcatag cttgcgtacc attaatcaat gctaaacctt ctttagcctg aaggttcaaa
    12841 ggttgtctat ttaattctct taatacatcg tcactatcct tttcttcccc tctgtacaat
    12901 actttccctt caccaattaa tgctaatgct aaatgtgata atggcgctaa atctcctgat
    12961 gcaccgagag agccttgctg tgggattatc ggtataatac gttcatttat aaaaaattgt
    13021 aattgtctca ctaattctaa agtggcacct gaatgacctt ttaataatgt attcaatcgt
    13081 aaaatcatca tgactaatgc tacttctttt gaaaatggct cacctagtcc acaggcatgt
    13141 gagcgtatca gattcacttg taattcatta tattgcgtcg ggtctatacg tacatcacta
    13201 aataacccaa aacctgtagt aataccgtaa accgtttcct cattttcaat aatacgttca
    13261 actaccgctc tacttttttt gacacgttct aacgcatcat caataatttc aatctttgat
    13321 tgttgttgta aaaatgattt aatatcctca attgttagtg tttcaccatc taaatataaa
    13381 gtcatatatg ttaccccctt gtttatatta agtaacccat ccttcttgaa gtatacgttt
    13441 tcatttttat tgaaacaatg gttttacgta catttataac ctattatcag agcactattg
    13501 tagtgcgtta aaggatatta agattgttgt aagcatattt aataatttat ctattgacga
    13561 attgcatata caggtatagt attttctatt gtatttaacg acaaataata atgaattcag
    13621 aaatttataa tacattttgt taaaagttac tatatatttt taaaattgaa taaattcgga
    13681 aaaggctttt acatgggagg ttatatcact atggaaacgt taaattctat taacattcct
    13741 aagcgtaaag aagattcaca taaaggtgat tatggcaaaa ttttattaat tggtggatct
    13801 gctaacttag gtggtgccat tatgttagcg gctcgtgcat gtgtatttag cggtagtggt
    13861 ttaatcactg tagctacaca tccaacaaat cattcagcat tacattctcg ttgcccagaa
    13921 gcgatggtta ttgatattaa tgatacgaaa atgttgacga aaatgattga aatgactgac
    13981 agtatactaa ttggtccagg tcttggcgtt gatttcaaag gaaataatgc cattacattc
    14041 ctactacaaa atatacaacc gcatcaaaat ttaatcgtag acggcgatgc gattacaatc
    14101 tttagtaaac tgaaaccgca attacctaca tgtcgtgtga tctttacacc acacctcaaa
    14161 gaatgggaac gattaagtgg tattcctatt gaggaacaga catatgagcg taatcgtgaa
    14221 gcagttgatc gtttaggtgc aactgttgta cttaaaaaac atggtactga aattttcttt
    14281 aaagatgaag actttaaatt gacaatcggt agcccagcaa tggcgactgg tggtatgggc
    14341 gatacacttg ctggtatgat tacaagcttt gtcggtcaat ttgataactt aaaagaagcg
    14401 gttatgagtg ccacatatac acatagtttt attggcgaaa accttgcaaa agatatgtat
    14461 gtggtgccac catcaagact tatcaatgaa ataccttacg caatgaaaca attagaaagt
    14521 tagtcattac taatcattga atatagtaaa gcattacttt ctagcataaa aataagactc
    14581 ccctacatat agggaagtct tattttttat tattcttcat ctgatgattg ttgtatatct
    14641 tcttcaacac gatccatgaa atcttgtctt acttcaatac gtccatcttc atcattttct
    14701 tctgaatcaa tcacttcagt atgaattgca tttcctggtg tttcatcatt tacaaccgct
    14761 tcacgttgtt gttcagtacc atcttcagat acagttgaag tagattgctc atcttcattc
    14821 gtttcatctt ctgcatcttc ttttacttta gcaaccgttg aaacaaattg atcatcacct
    14881 aagcgaatta agcgaacacc ttgtgctgca cgaccatttt gagaaatatc tgcaacatct
    14941 agtcgaataa tgacacctgc attagtaaca atcattaaat cttcttcacc agttactgta
    15001 gtgatacata caacattacc attacgctca gtaatcgtag ctgttttaat acctttacca
    15061 ccacgatttg ataagcgata gtcattaact ggcgtacgtt taccataacc attttcagta
    15121 actactaata cttcatcaac actgtttgca tgagctacat caagccctac aacttcgtca
    15181 ccttcacgaa gtgtaatacc tttcacaccc gttgctgtac ggcctaaagg acgtaatgtt
    15241 gattcaggga atcgaattaa tgatgcatgt gatgtaccaa tcaagatatc ttcttgacca
    15301 cttgttaagc gaactgcaat taactcatca tcttctctga acgaaatcgc aatcttacca
    15361 tttctattta ttcttgagaa gttacttaat gctgaacgtt taacgacacc acgtttagtt
    15421 gcaaacacta agaagttgtc ttcactttca aggtctttaa cagcaatcat tgtactaatg
    15481 acttcatcat tttcaagttc aatagcattc actacaggaa tacctttaga ctgtcttgat
    15541 aactcaggca cttcgtaacc tttaagtttg tatacacgac ctttgttagt aaagaacaat
    15601 acatggtcat gtgtacttaa agttaccaat tgactgacaa aatcttcttc caatgtattc
    15661 ataccttgaa caccacgacc accacggttt tgagcacgat atgtagatac cggcaaacgt
    15721 ttaatgtagt tattatggct tagtgtaatt actatttgtt cttctggaat taagtcttcg
    15781 tcctctaagt tttcaaatcc acctaattga atttctgtac gacgatcatc accgaaacga
    15841 tctctaattt cagtcaattc atctctaact aactgtaata acacttcttc atcagctaag
    15901 attgcttcta attcactaat ataatttaat aactcattat attcagcttc aattttgtct
    15961 ctctctaaac ctgttagacg tcttaaacgc atgtctaaaa tagcttgagc ttgtttttca
    16021 gaaagtttga agcgttgttg caagctttcc attgcaactt tatctgtatc tgactcacga
    16081 atcgttgaaa taatttcatc gatatggtca agtgcgatac gtaatccttc taaaatgtgg
    16141 gcacgatctt tagctttacg taagttgtat tgcgtacgtc ttctaacaac tgtcttttga
    16201 tgctctaaat aatgtaccaa cgcttctttt aaattaataa gcttcggtct accatttaca
    16261 agtgcaatca tattcacacc aaatgatgtt tgaagaggtg tttgtttgta taagttattt
    16321 aaaatgacac tagcatttgc atccttacgc acatcaataa cgacacgcac accagtacgt
    16381 aaacttgttt catcacgtaa atcagtgata ccgtcaattt tcttgtcacg aacgagctct
    16441 gcaatttttt caatcatacg agccttattc acttggaaag gaatttcagt gacaacaata
    16501 cgttgacgtc cgcctccacg ttcttcaata actgcacgag aacgcatttg aattgaacca
    16561 cgacctgttt catatgcacg tctaatacca ctcttaccta aaataagtcc agcagttggg
    16621 aaatcaggac cttcaatatc ctccattaac tcagcaattg aaatatcagg gttcttactt
    16681 aagctaagta caccattgat taattctgtt aagttatgtg gtggaatatt cgttgccata
    16741 cctaccgcga tacctgatgc accattggct aataagttag ggaatcgagc aggtaagact
    16801 gacggctctc tttcattacc atcatagtta tcgataaaat ctattgtatc tttattaata
    16861 tcacgtaaca gttcaagtgt gattttagtc atacgcgctt cagtataacg cattgctgct
    16921 gcgccatctc catccattga accaaagtta ccttggccat caacaagcgg ataacgataa
    16981 ctgaaatctt gagccatacg taccattgct tcataaatag ataagtcacc atgagggtga
    17041 tatttaccca ttacgtcacc aacgatacgt gctgattttt tatatgattt atccggtgtc
    17101 ataccttgtt catttaatcc atatagtata cgacgatgta ctggttttaa accgtcacga
    17161 acatctggca atgcacgagc aacgataaca ctcatcgcat aatctaaaaa tgattcacgc
    17221 atttcactgg taatatttcg ttcatttatt cttgattgag gtaattcagc catcaagagt
    17281 tcctccttca aaagttcagt tcacagcgct tagaagtcta agtttgcata aactgcatta
    17341 tcttctataa attgtctacg gttttctaca acgtcaccca ttaacatttc aaatgtttgg
    17401 tccgcttcaa tcgcatcttc aagttttact tgtaaaagag cgcggtgctc agggttcatt
    17461 gttgtttccc ataattgatc tgcattcatt tctccaagac ctttgtatcg tgcaatagac
    17521 cattttggtg ttggattcaa ttcagattta agtttatcaa gttccctatc attgtataca
    17581 taatactttt gtttaccttg tgtcagttta tacaacggtg gctgtgcaat atacacatag
    17641 cctgcttcaa ttaacggtct cataaatcga tagaagaatg ttaataacaa tgttctaata
    17701 tgcgctccat ccacatcggc atcagtcata atgacgattt tgtgatatct tgctttcgct
    17761 agatcaaagt cgccaccgat tcctgtacca aatgctgtga tcatttgacg aatttcattg
    17821 ttattcaaaa ttctatctaa tcgtgctttt tcaacattta atatcttacc tcgtaatggt
    17881 aaaatcgcct gcgttctaga gtcacgacca gattttgtag accccccggc agagtcccct
    17941 tcgactaaga aaatctcaca ttcttcagga cttttactag agcaatcggc taatttacct
    18001 ggaaggcttg ctacatctaa cgctgattta cgacgtgtta cttcacgcgc ttttttcgca
    18061 gcaacacgtg cacgtgccgc cataatacct ttttcaacca ctgtacgtgc gacttgtgga
    18121 ttttcatata aaaatcgttc aaagtgctct gagaataatt tatctacaac ttgacgcact
    18181 tcagaattac ctaattttgt cttcgtttga ccttcgaatt gaggatcacc atgtttgata
    18241 gatataattg ctgtcatacc ttcacgtgta tcttcaccag aaagtctatc tttttcttct
    18301 ttcataatct tgctacttaa accataacta tttaagacac gcgttaatgc acgtttgaat
    18361 ccgtcttcat gcgtaccacc ttcatacgta tgaatgttat ttgcgtaagt taaaagattt
    18421 gtggcatatc ctgagttata ttgaatcgca atttctactt caatatcatc tttagattga
    18481 tgaatataaa ttggctcatc atgaataggt tctttatttt cgttcaataa ctcaacgtac
    18541 gatttaatac cgccctcata gtgataggag tcttctctaa cgttttcttc atcacgttca
    18601 tctcttaatg tgatttgaat tcctttgttt aagaaagcaa gctctctaat acgctgctgt
    18661 aatgtttcat agttgtatac agttgtctct gtgaagattt ctccatctgc tttaaaacga
    18721 atgacagtac ctgtcttatc agttgtgcca acttctttta agtcaaattg aggtacacct
    18781 tttttatatg cttgatgata tatagtctca tttctgtgta catatacttc taagtcttgt
    18841 gacaatgcgt ttacaactga tgaaccaaca ccatgtaaac caccagatac tttgtatccg
    18901 ccaccgccaa atttaccacc agcatgtaaa acagttaaaa taacttcgac agctggacgt
    18961 cccatttttt cttgaatatc aactgggata ccacgtccgt tatccgttac tttaatccag
    19021 ttatcttttt caataacaac ttcaatttga tttgcataac cagctaatgc ttcatcgata
    19081 ctattatcga caatttccca cactaaatgg tgcaaacctc tctctgaagt cgatcctata
    19141 tacatacctg gtcttttacg tactgcttct aaaccttcta atacttgtat ttgcccagca
    19201 ccataattat ccgtgttgtt tacatctgac aatgcagtca ccatcgcttt ctgttacttt
    19261 ataatttcac cttgattaat acgatacaat ttagcgttat tcatgatttc atgatcaata
    19321 ccatctacag atgtcgtagt gacaaatgtt tgtactttat gctgaatcgt acttaataaa
    19381 tgcgtttgac gcgaatcatc taattcactg agtacatcgt ctaataataa gatgggatat
    19441 tccccaactt cgatattcat taactcaatt tcagctaatt taatggacaa agccgttgta
    19501 cgttgctgtc cttgagaacc atatgtttga gcatccatgc cattcacatc aaaacttata
    19561 tcatctcgat gtggtccgaa taagctaatg cctcgttctt tttctctttg catattatcg
    19621 ctaagaatag acataatttc ttcaagtcgt gccgcttcat tttgagcata atcaaattta
    19681 agactaggta aataattcag cgacaacgct tctttatcat ttgtgatacc agcatgaatc
    19741 ggtttagcta acgactctag ctcttgaata aaatgtgcac gtttatcagt tactttcatt
    19801 gcatattcag caaactgctg atttaatact tccaacattg ttaagtcctt tttttggcct
    19861 aattgtaact gctttaagta attattcttt tgctttaaaa tacgttggta ttgagctaaa
    19921 tcatttaagt aaacagcaga aatttggccc aactccatat ctataaagcg tcgtcttatt
    19981 tgaggagagc cttttacaat attcaaatct tctggcgcaa atagaaccac attgaggtgt
    20041 ccaatatatt gagttagacg actttgctct aagtgattca ctttgacttg tttacctttt
    20101 ttagttataa acattgttaa tggcatcgtg ccgtgtctat aactaagctc accttctatt
    20161 ttagcataat cagcattaaa acgtatgagt tccttatcat tactcgttct atgacttttt
    20221 gctaaagcta aggtataaat tgattcaagt aaatttgtct ttccttgtgc attttctcca
    20281 atgaggatat tcacgtcagg atgacatttc aacgtaacct catcatagtt acgataattt
    20341 tctaattgga gtgtatttaa cttcattgtt caccttgatg aatgattaag aaagaaccag
    20401 catcttcagg taattctggg atatctatac gatcttgatg ttctaacttt ttaccgcgac
    20461 gtgtttcacg cactccatta attaatactt caacgtcttg caagaaccat tttgcttgac
    20521 caccagattc aataatccct tctgttttta gaaattgacc taaattaatg tctccttcta
    20581 ctacaacttc ttgaaccaaa ataatcactc catttctttg tatgtcatta atatattata
    20641 cctttttttg tcacaaattt catttaaaat agaggtagaa accgtttaca tataaataat
    20701 taattcttat tttttggcaa tactgtcatt ttcaattttt taacgctatt taacggcgtt
    20761 taacttttat attcaacttg ttattgact
//
LOCUS       DAKTLH010000033        19984 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.033, whole genome shotgun sequence.
ACCESSION   DAKTLH010000033 DAKTLH010000000
VERSION     DAKTLH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19984
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.033"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            16..1071
                     /locus_tag="O0394_001037"
     CDS             16..1071
                     /locus_tag="O0394_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCY0499126.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
     gene            complement(1241..1882)
                     /locus_tag="O0394_001038"
     CDS             complement(1241..1882)
                     /locus_tag="O0394_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCY0499127.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDIGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            2026..2892
                     /gene="fakB1"
                     /locus_tag="O0394_001039"
     CDS             2026..2892
                     /gene="fakB1"
                     /locus_tag="O0394_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HCY0499128.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            3256..4338
                     /locus_tag="O0394_001040"
     CDS             3256..4338
                     /locus_tag="O0394_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCY0499129.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRK
                     RQTILLYAVTGAGKTEMMFQGIQYARIQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNKKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGV
                     SMNMIQAKKEIQRMNKLALKRGWIDE"
     gene            4331..5005
                     /locus_tag="O0394_001041"
     CDS             4331..5005
                     /locus_tag="O0394_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCY0499130.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKHLNQDEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFLKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            5066..5638
                     /gene="raiA"
                     /locus_tag="O0394_001042"
     CDS             5066..5638
                     /gene="raiA"
                     /locus_tag="O0394_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCY0499131.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            6052..8583
                     /gene="secA"
                     /locus_tag="O0394_001043"
     CDS             6052..8583
                     /gene="secA"
                     /locus_tag="O0394_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCY0499132.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            8625..8729
                     /locus_tag="O0394_001044"
     CDS             8625..8729
                     /locus_tag="O0394_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499133.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKKSV"
     gene            8898..10008
                     /gene="prfB"
                     /locus_tag="O0394_001045"
     CDS             join(8898..8969,8971..10008)
                     /gene="prfB"
                     /locus_tag="O0394_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCY0499134.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            10162..10269
                     /locus_tag="O0394_001046"
     CDS             10162..10269
                     /locus_tag="O0394_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499135.1"
                     /translation="MCLLKQHMQCAFSNITYGDFQGIELIIENVLNYMV"
     gene            10417..11256
                     /locus_tag="O0394_001047"
     CDS             10417..11256
                     /locus_tag="O0394_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0499136.1"
                     /translation="MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKSPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVTKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            11430..12080
                     /locus_tag="O0394_001048"
     CDS             11430..12080
                     /locus_tag="O0394_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCY0499137.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            12077..12313
                     /locus_tag="O0394_001049"
     CDS             12077..12313
                     /locus_tag="O0394_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HCY0499138.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            12582..14567
                     /gene="uvrB"
                     /locus_tag="O0394_001050"
     CDS             12582..14567
                     /gene="uvrB"
                     /locus_tag="O0394_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCY0499139.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            14575..17421
                     /gene="uvrA"
                     /locus_tag="O0394_001051"
     CDS             14575..17421
                     /gene="uvrA"
                     /locus_tag="O0394_001051"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCY0499140.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     HDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            18083..19015
                     /locus_tag="O0394_001052"
     CDS             18083..19015
                     /locus_tag="O0394_001052"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCY0499141.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            19021..19860
                     /locus_tag="O0394_001053"
     CDS             19021..19860
                     /locus_tag="O0394_001053"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCY0499142.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPSEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGSVSRAFLEQLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIGR
                     FFIEGLRTDSLMLTSNIRVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPTK
                     KVK"
     gene            19868..>19984
                     /locus_tag="O0394_001054"
     CDS             19868..>19984
                     /locus_tag="O0394_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0499143.1"
                     /translation="MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSKF"
BASE COUNT         7199 a         2781 c         3813 g         6191 t
ORIGIN      
        1 gatgaaggtg aataaatggt tacattatta ctagttgcag taacaatgat tgtcagtttg
       61 acgataacac caattgttat tgcaatatcg aaaagattaa atttagttga taaaccaaat
      121 tttagaaaag tacacactaa acctatttca gttatgggtg gtacagtgat tctcttttca
      181 tttttaatag gtatttggat tggtcatcct attgaaacag aaatcaaacc acttattatt
      241 ggtgcgatta ttatgtacgt acttgggctt gtagatgata tctacgattt gaaaccgtat
      301 ataaaattgg ctggtcaaat tgccgctgcc ttagtagttg ctttttatgg tgtgactatt
      361 gattttattt cgttgccaat gggtacaacg attcattttg gatttcttag tattccaatt
      421 actgtgattt ggattgttgc tattacaaat gcaattaact taattgatgg actcgatggt
      481 ttggcgtcgg gtgtttctgc aatcggactc attacaatag ggttcattgc aattttacaa
      541 gctaatattt tcataacgat gatttgttgt gttttattag gctctttaat tgggttttta
      601 ttttacaatt tccatcctgc caaaatattt ttaggtgata gtggggcttt aatgattgga
      661 tttatcatcg gattcctttc tttactcgga ttcaaaaata ttacaattat tgcattgttc
      721 ttcccaattg ttatcttagc agttccattc attgatactt tgttcgcaat gattcgacgt
      781 gtgaaaaaag ggcagcatat aatgcaagct gataaatcgc atttgcatca taaactatta
      841 gctttaggct acacacatag acaaacagta ttattaatct attcaatctc tattttattt
      901 agtctttcga gcattatttt gtatgtatcg ccaccattag gtgttgtatt aatgtttgta
      961 ttaatcatat ttagtattga attaattgtt gaatttacag gattaataga taacaactac
     1021 cgaccaatat taaatttaat tagtcgtaag tcatctcata aagaggaata gggaatgaaa
     1081 gcatagctgt atgggataat ttgtattata tggctttact ctttacaatt tttttgtatt
     1141 aaatttcaaa tataaaaagc actgccataa acgtgtactt caattgtcgt ttaataatac
     1201 gcaattgata tttaccgtct tatgatagtg ctttttattt ttattcagtt ggtatatcga
     1261 aaggtaactg ctttggagtt tcttcagtca aatcgaaatt tcctgcagtc atttgattta
     1321 aaaagttaat aaacgcttca tagtcacttt taacgacatc gatatagtag cttaccttat
     1381 cagtgtaagt ttggtttctt aacataaaat gagttgaagc taattcatat tcaaatttac
     1441 cagtttgatc ataattcagt gttactatac atggtactgc ttctcgtagt tcgacacgcc
     1501 cgatatcata aatgacgtct ctaacagcac cgctataggc gcgaattaaa ccgccaccac
     1561 ctaatttaat accaccaaaa tatcttgtta ctacgacaca cgcattatga acatcgagct
     1621 tttttaatat gtctaacatt gggacaccgg cagttcctgt cggttcacca tcatcattcg
     1681 ctttttgaat attcatttca ggtccaatag tatatgcaga acaattatga gtggcatctt
     1741 tatgttcttt ttttattgca gcaataaatg ctttagcttc atcttcattt tgaacaggtt
     1801 tgatatgagc aatgaatctt gatttactaa tcacattttc aataatgtgt tcttttttaa
     1861 cagtaatgat attttgtgtc ataataactc cttaattcat aagcttaaga ttatttaatc
     1921 ttcattatac actgaaaatg acatgactat aaatcgtttg attgccattt tctttttaac
     1981 tgaaatattg tatcattgct atgagtatat tttaggagga cgactatgaa aattgctgtg
     2041 atgaccgatt ctacaagtta tctgtcgcag gacttaatcg ataaatataa tattcaaata
     2101 gcgccattaa gtgtgacttt tgatgatggg aagaacttta cagaaagtaa tgaaatagca
     2161 attgaagaat tttataataa aatggcatcg tctcaaacga ttccaacaac aagccaacca
     2221 gcaattggcg aatggattac taaatatgaa atgttaagag atcaaggtta cacagatatc
     2281 attgtcattt gcttatcaag tgggattagt ggaagttatc aatctagtta tcaagcaggg
     2341 gaaatggttg aaggtgttaa tgtacatgca tttgatagta agcttgcagc aatgattgaa
     2401 ggatgttatg tattacgtgc tattgaaatg gttgaagaag gatacgagcc acagcaaatt
     2461 attgatgatt taactaatat gcgtgaacac acaggtgcat atttaattgt tgatgactta
     2521 aagaatttac aaaaaagtgg tcgaattact ggtgctcaag catgggttgg aacattattg
     2581 aaaatgaagc cagttcttaa gtttgaagat ggcaagatta taccagaaga aaaagttcgt
     2641 actaaaaagc gtgccattca aacattagaa aagaaagtat tagatattgt aaaagacttt
     2701 gaagaagtaa ctttatttgt cataaatgga gatcatttcg aagatggtca agcgttatac
     2761 aaaaagttac aagatgattg tccttcagct tatcaagtag catactctga gtttggtcca
     2821 gttgttgcag cacatttagg ttctggtgga ttaggtttag gctatgttgg cagaaaaata
     2881 agattaacat aattataaaa ttttaataaa agagtctata ttgtaattgg aaattatctc
     2941 tcgtatacat ggctttaaat gttcatcatt tgaaagccaa aatgctaaag atataagaaa
     3001 atcattataa tattaggctc ttttttacgt tgaaatgagg ttttaagcat taaacattac
     3061 gggaaattaa ttcatcctca tacttcactt actaatgaaa aaattaaaaa agaagtaaca
     3121 ggtgtcatca aacaaaattc aaactattat tgtgttcaat gtgaaagtac aaatccaaag
     3181 catttttatc agtatgattc ctcagtacat tccaagaaaa ttgtatattg cagaaattgt
     3241 atatcactgg gtcgaatgga taatgtaaca agatataaaa taacagagag ttcgcaaagt
     3301 tcatcacaag catattatca tctctcattt gaattgtcgg aacagcagtc ttatgcctca
     3361 gaacatattg ttcgagccat tagaaagaga caaacgattt tgttatatgc cgtaacaggt
     3421 gcaggtaaga cagaaatgat gtttcaaggc attcaatatg caagaataca gggagataat
     3481 atagctattg tgtcaccacg tgtagatgtt gttgtagaaa ttagtaaacg tattaaagac
     3541 gcatttctta atgaagatat agacatacta caccagcaat caagacaaca atttgaaggg
     3601 cattttgttg tatgcacagt gcatcaactt taccgattca aacagcactt tgatactatt
     3661 tttattgatg aagtcgatgc ctttccttta tcaatggata aaaatttaca acaagcattg
     3721 aagtcatctt ctaaagttga acatgcaaca atttatatga cagcaacacc accgaaacaa
     3781 cttctgtcag agattcccca cgaaaatata attaaattgc cagctcgctt tcataaaaaa
     3841 tcacttccag ttcctaaata tcgttatttc aaacttaata ataagaagat tcagaaaatg
     3901 ttataccgaa ttttacaaga tcaaattaat aatcaacgtt atacactggt gttttttaac
     3961 aatatagaaa caatgattaa aacattttcg gtttataagc agaaaattac taaattaaca
     4021 tacgtccata gcgaggatgt ttttcgcttt gaaaaagttg aacaattaag gaatggacat
     4081 ttcgatgtca tttttactac gacaatatta gaacgtggat ttacaatggc aaatttggat
     4141 gttgttgtta tcgatgcaca tcaatatact caagaggctt taatacaaat tgctggacgt
     4201 gttggacgaa aattagaatg tcctactgga aaagtattgt tttttcatga aggggtaagt
     4261 atgaatatga ttcaagctaa aaaagagatt caaaggatga acaaattagc attaaaaaga
     4321 ggttggattg atgaataatt gtttgagttg tggtgctaag ttatatgaaa atataaccat
     4381 ttataatttg ttcaagaaac ctaatagatt atgtgacaga tgcaaagaga attgggacaa
     4441 tattaaactt gatattaaag caaggcgatg ttcaaggtgc ttaaaacact taaatcaaga
     4501 tgaagcgtat tgtttagact gcaagtttct atcggcacac tttaatttaa tggaacaatt
     4561 atattgtcaa tttcaatatg acggtttaat gaaagagatg atacatcagt ataaattttt
     4621 gaaagactat tatttatgtg aattattggc acatttgatt gaaataccac aaacatctta
     4681 tgactatatt gtgccaattc cttcttcgcc ggcacatgat ttatctagaa catttaaccc
     4741 ggtagaagca gtactaaaag ctaaagggat tcgctttgat aagattttaa agatgtcaaa
     4801 tagaccaaaa cagtctcatt taactaagaa agagcgtctg gcagatgaaa atccatttat
     4861 tattgatacg gaattagatt taaatggtaa ggaaatatta ctcgttgacg atatttatac
     4921 aactggatta acaattcatc gtgcagggtg taaattatat gctaaaaata tcagaaaatt
     4981 caaagtgttt gcgtttgcac gatagcgtaa aaatgttaaa atataataaa gagttaccaa
     5041 taaagaggtt taaggagaga ttactatgat tagatttgaa attcatggag ataacctcac
     5101 tatcacagat gctattcgca actatattga ggaaaaaatt ggtaagttgg aacgttattt
     5161 taatgacgta ccaaatgcag tggcgcatgt taaagttaaa acttattcaa attcagctac
     5221 taaaattgaa gtaacaattc cattgaaaaa tgttacgtta agagctgaag agcgaaacga
     5281 tgatttatac gcaggtattg atttaattaa taataaactt gaaagacaag ttcgaaaata
     5341 taaaacacgt attaatcgta agagccgtga tcgaggagat caagaagtgt ttgttgccga
     5401 attacaagaa atgcaagaaa cacaagttga taatgacgct tacgatgata acgagataga
     5461 aattattcgt tcaaaagaat tcagcttaaa accaatggat tcagaagaag cggtattaca
     5521 aatgaatcta ttaggtcatg acttctttgt attcacagac agagaaactg atggaacaag
     5581 tatcgtttac cgccgtaaag acggtaaata tggcttgatt caaactagtg aacaataaat
     5641 taagtttaaa gcacttgtgt ttttgcacaa gtgctttttt atactccaaa agcaaattat
     5701 gactatttca tagttcgata atgtaatttg ttgaatgaaa catagtgact atgctaatgt
     5761 taatggatgt atatatttga atgttaagtt aataatagta tgtcagtcta ttgtatagtc
     5821 cgagtcgaaa atcgtaaaat atttataata taatttatta ggaagtataa ttgcgtattg
     5881 agaatatatt tattagtgat aaacttgttg acaacagaat gtgaatgaag tatgtcataa
     5941 atatatttat attgattcta caaatgagta aataagtata attttctaac tataaatgat
     6001 aagatatatt gttgtaggcc aaacagtttt ttagctaaag gagcgaacga aatgggattt
     6061 ttatcaaaaa ttcttgatgg caataataaa gaaattaaac agttaggtaa acttgctgat
     6121 aaagtaatcg ctttagaaga aaaaacggca attttaactg atgaagaaat tcgtaataaa
     6181 acgaaacaat tccaaacaga attagctgac attgataatg tcaaaaagca aaatgattat
     6241 ttagataaaa ttttaccaga agcatatgca cttgttagag aaggctctaa acgtgtattc
     6301 aatatgacac catataaagt tcaaattatg ggtggtattg caattcataa aggtgatatc
     6361 gctgagatga gaacaggtga aggtaaaaca ttaacagcga caatgccaac atacttaaat
     6421 gcattagctg gtagaggtgt tcacgttatt acagtcaatg aatacttatc aagtgttcaa
     6481 agtgaagaaa tggctgagtt atataacttc ttaggtttga ctgtcggatt aaacttaaac
     6541 agtaagacga cagaagaaaa acgtgaagca tacgcacaag acattactta cagtactaat
     6601 aatgagctag gttttgatta cttacgagat aacatggtga attattctga agatagagta
     6661 atgcgtccat tacattttgc aatcattgat gaggttgact caattttaat cgacgaggca
     6721 cgtacgccat taattatttc tggtgaagct gaaaagtcaa cgtcacttta tacacaagca
     6781 aatgtttttg cgaaaatgtt aaaacaggac gaagattata aatacgatga aaaaacgaaa
     6841 gctgtacatt taacagaaca aggtgcggat aaagctgaac gtatgttcaa agttgaaaac
     6901 ttatatgatg tacaaaatgt tgatgttatt agtcatatca acacagcttt acgtgcgcac
     6961 gttacattac aacgtgacgt agactatatg gttgttgatg gcgaagtatt aattgtcgat
     7021 caatttacag gacgtacaat gccaggccgt cgtttctcgg aaggtttaca ccaagctatt
     7081 gaagcgaagg aaggcgttca aattcaaaat gaatctaaaa ctatggcgtc tattacattc
     7141 caaaactatt tcagaatgta caataaactt gcgggtatga caggtacagc taaaactgaa
     7201 gaagaagaat ttagaaatat ttataacatg acagtaactc aaattccgac aaataaacct
     7261 gtgcaacgta acgataagtc tgatttaatt tacattagcc aaaaaggtaa atttgatgca
     7321 gtagtagaag atgttgttga aaaacacaag gcagggcaac cagtgctatt aggtactgtt
     7381 gcagttgaga cttctgaata tatttcaaat ttacttaaaa aacgtggtat ccgtcatgat
     7441 gtgttaaatg cgaaaaatca tgaacgtgaa gctgaaattg ttgcaggcgc tggacaaaaa
     7501 ggtgccgtta ctattgccac taacatggct ggtcgtggta cagatatcaa attaggtgaa
     7561 ggcgtagagg aattaggcgg tttagcagta ataggtacag agcgacatga atctcgtcgt
     7621 attgatgacc agttacgtgg tcgttctgga cgtcaaggtg ataaagggga tagtcgcttc
     7681 tatttatcat tacaagatga attaatgatt cgttttggtt ctgaacgttt acagaaaatg
     7741 atgagccgac taggtttaga tgactctaca ccaattgaat caaaaatggt atcaagagct
     7801 gtagaatcag cacaaaaacg tgtagaaggt aataacttcg acgcgcgtaa acgtatctta
     7861 gaatacgatg aagtattacg taaacaacgt gaaattatct ataacgaaag aaatagtatt
     7921 attgatgaag aagacagctc tcaagttgta gatgcaatgc tacgttcaac gttacaacgt
     7981 agtatcaatt actatattaa tacagcagat gacgagcctg aatatcaacc attcatcgac
     8041 tacattaatg acatcttctt acaagaaggt gacattacag aggatgatat caaaggtaaa
     8101 gatgctgaag atattttcga agtcgtttgg gctaagattg aagcagcata tcaaagtcaa
     8161 aaagatatct tagaagaaca aatgaatgag tttgagcgta tgattttact tcgttctatt
     8221 gatagccatt ggactgatca tatcgacaca atggatcaat tacgtcaagg tattcactta
     8281 cgttcttatg cacaacaaaa tccattacgt gactatcaaa atgaaggtca tgaattattt
     8341 gatatcatga tgcaaaatat tgaagaagat acttgtaaat tcattttaaa atctgtagta
     8401 caagttgaag ataatattga acgtgaaaaa acaacagagt ttggtgaagc gaagcacgtt
     8461 tcagctgaag atggtaaaga aaaagtgaaa ccgaaaccaa tcgttaaagg cgatcaagtt
     8521 ggtcgtaacg atgattgtcc atgtggtagt ggtaaaaaat tcaaaaattg ccatggaaaa
     8581 taaatgatat aaaataactc cttccaatta aacacctata gtttgtgtta tgggaggagt
     8641 ctttttattt tacaagcgtt aaatacttta aaaaatgtga agaagttgtt aaacgttgtt
     8701 atgtacttag ttttaaaaaa tcggtttagg catatgtcga tgataaatgt actgattttt
     8761 aacaataaat gcataaacta attgtcagtg tgcttatatt tcttaacatt gttatttaac
     8821 aaaattatgt taaaatttag cattataaaa gatgcaaatc aatgacttga attgaaatat
     8881 aaataggagc gaatgctatg gaattatcag aaatcaaacg aaatatagat aagtataatc
     8941 aagatttaac acaaattagg gggtctcttt gacttagaga acaaagaaac taatattcaa
     9001 gaatatgaag aaatgatggc agaacctaat ttttgggata accaaacgaa agcgcaagat
     9061 attatagata aaaataatgc gttaaaagca atagttaatg gttataaaac actacaagca
     9121 gaagtagatg acatggatgc tacttgggat ttattacaag aagaatttga tgaagaaatg
     9181 aaagaagact tagagcaaga ggtcattaat tttaaggcta aagtggatga atacgaattg
     9241 caattattat tagatgggcc tcacgatgcc aataacgcaa ttctagagtt acatcctggt
     9301 gcaggtggca cggagtctca agattgggct aatatgctat ttagaatgta tcaacgttat
     9361 tgtgagaaga aaggctttaa agttgaaact gttgattatc tacctgggga tgaagcgggg
     9421 attaaaagtg taacattgct catcaaaggg cataatgctt atggttattt aaaagctgaa
     9481 aaaggtgtac accgactagt acgaatttct ccatttgatt catcaggacg tcgtcataca
     9541 tcatttgcat catgcgacgt tattccagat tttaataatg atgaaataga gattgaaatc
     9601 aatccggatg atattacagt tgatacattc agagcttctg gtgcaggtgg tcagcatatt
     9661 aacaaaactg aatcggcaat acgaattacc caccacccct caggtatagt tgttaataac
     9721 caaaatgaac gttctcaaat taaaaaccgt gaagcagcta tgaaaatgtt aaagtctaaa
     9781 ttatatcaat taaaattgga agagcaggca cgtgaaatgg ctgaaattcg tggcgaacaa
     9841 aaagaaatcg gctggggaag ccaaattaga tcatatgttt tccatccata ctcaatggtg
     9901 aaagatcatc gtacgaacga agaaacaggt aaggttgatg cagtgatgga tggagacatt
     9961 ggaccattta tcgaatcata tttaagacag acaatgtcgc acgattaata tatattttaa
    10021 aaccgaggct ctaaaagggc gtcggttttt ggttttttta aaggtagcta aataaattgt
    10081 aaattagatt ttggaatatg atttgtttat gaatatttaa gtacaattcg gtagatagag
    10141 ttagaatata ttttttaaaa gttgtgtttg ttaaaacaac atatgcagtg tgcatttagt
    10201 aatattacct atggcgattt tcaaggtatt gaattaatta ttgaaaacgt tctcaattac
    10261 atggtatgaa tacattttac actatgataa aaggttgtat tctttttata ttgttaacca
    10321 tttgattaca tcgttataac aatagctttt gacaaaatgt attgtgctat agtatttgca
    10381 tacttaaaat actaacagca aaggaatgac agcaagatga aaaaatctct tacagtgacg
    10441 gtttcgtcag tgttagcttt tttagcttta aataatgcag cacatgcaca acaacatggc
    10501 acacaagtaa aaacacctgt tcaacataat tatgtatcaa atgttcaagc acaaacgcaa
    10561 tcaccgacaa cttatacagt agttgctggc gattcattat ataagattgc tttagagcat
    10621 cacttaacgt tgaatcaatt atattcatac aatcctggtg taacaccttt aatttttcct
    10681 ggtgacgtga tttcacttgt gcctcaaaat aaagtgaaac aaactaaagc ggttaaatca
    10741 ccagtaagaa aagcaagcca agctaaaaag gtagtaaaac aacctgtaca acaagcatct
    10801 aaaaaagtag tagttaagca agcacctaag caagcagtaa ctaagacagt taatgtagca
    10861 tacaaacctg ctcaagtaca aaaatcagta ccaactgtac ctgttgcaca taactacaat
    10921 aaatcagttg ctaacagagg aaacttatat gcttatggaa actgcacata ttatgctttc
    10981 gatcgtcgtg cacaattagg tagaagtata ggaagtttat ggggcaatgc aaataactgg
    11041 aattacgcag caaaagttgc aggatttaaa gtagataaaa caccagaagt tggcgctatt
    11101 ttccaaacag ctgctggtcc atatggacat gttggtgttg ttgaatctgt aaaccctaat
    11161 ggaacaatta ctgtttctga aatgaactat gctggattta atgttaaatc ttcaagaaca
    11221 attttaaatc caggaaaata taattacatc cactaagtaa tatatcaaga caagactatc
    11281 ctcttagcct gtttaagtaa caggttgaga ggattttttg gtatcattta atcagagtta
    11341 tataaagaag atatttaaat gatatttaat tgattgatat gtaaaaagaa agtataattt
    11401 atgattaatt aatggaggag gtaattgaaa tgggtgtaca tcaatatttt aaaagattat
    11461 cagatatgga aagacttata agattacctg gaaaatttaa atattttgaa cacaatgttg
    11521 cagcacactc ctttaaagta actaaaattg cacaatatct agcaacagtt gaagaatatc
    11581 atggacgaaa gattaattgg aaaagcttat atgaaaaagc attaaatcat gatttcgccg
    11641 aagtgtttac tggtgatata aaaacacctg ttaaatatgc gagtagtgaa ttaaaaaaat
    11701 tattttcgca agttgaagaa gaaatggtag agacctttat tgaagaagaa attccattac
    11761 aatatagaga tgtttataag caacgactgc aagaaggtaa agatgattca ttagaaggcc
    11821 aaatactttc agttgctgat aaaattgatt tgctttatga aacatttgga gaaatacaaa
    11881 aacgtaatcc cgaagaatta tttttcgaaa tttatgaaat gagtctagaa acaattattc
    11941 aatttgacca tttagcatct gtacaagatt ttattaataa tatcattcca gaaatgttga
    12001 ctgaaaactt tatacctaga acagaattaa gagaaacaac catgaacatt ttaaataaaa
    12061 gaaaagagga aaatgaatga tatggtattt tagcgcagca ttctttccat gtgtcttggt
    12121 agtattattt agtgtaataa caagaagtaa atgggtcggt actattctga cattaatttt
    12181 aattggtgcc tcaatctata aagagtattt ccataacgag tggattattt ttattgatgt
    12241 agtgtcatta ttagctggtt atttaattat agatcaactc gaatttcata aacatcaaga
    12301 tgaagatcgc taagattaac tttaaaataa tgtttcaaac aaatttgttg aaacaaaatg
    12361 atgattaata taatgtgtat ttacatacta aaaataacaa gataaacgat ttgatttaag
    12421 gcaagcatag ttagcacaac tatgtttgtt tttctttgtt cgacattttt acgaacaaac
    12481 gtttgctttt tgtgtgacta ctttgctaaa atatgtaatg agaaagcaaa gagttgagcg
    12541 gaaatataaa taatacgttg aaagaggagg catatgtgac aatggttgaa cattatcctt
    12601 ttaaaataca ttctgatttt gagcctcaag gtgaccaacc gcaagcaatt aaagaaatcg
    12661 tggaaggtat taaagcgggg aaaagacatc aaactttatt aggtgctact ggcacaggga
    12721 aaacatttac gatgagtaat gttattaaag aagttgggaa accaacgtta attatcgcgc
    12781 ataacaaaac attagcagga caattatata gtgagtttaa agaatttttt cctgaaaaca
    12841 gggtggaata ctttgtaagt tactatgatt attatcaacc agaggcatac gtaccgtcta
    12901 ctgacacttt tattgaaaaa gatgcctcaa tcaatgatga aattgatcaa ctacgacatt
    12961 ctgctacaag tgcattattt gaacgcgatg atgtaattat tattgctagt gtaagttgta
    13021 tatatggttt aggtaatcct gaagaatata aagatttagt agtaagtgtt cgagttggta
    13081 tggaaatgga tagaagtgaa ttacttagaa aacttgtaga tgtgcaatat acacgaaatg
    13141 acatcgattt ccaacgagga acgtttcgag tgcgtggtga tgtagtggaa atattcccag
    13201 cctctaaaga agaactttgt ataagggttg agtttttcgg cgatgagatt gaccgtatcc
    13261 gagaagttaa ctacctaaca ggtgaagtgt tgaaagaaag agaacatttt gcgatattcc
    13321 cagcttctca cttcgtaaca cgtgaagaaa agttgaaagt tgcgattgaa cgtattgaaa
    13381 aagaattgga agaacgattg aaagaattac gagatgagaa taaattacta gaagcgcaaa
    13441 ggttagaaca gcgtaccaac tatgatttag aaatgatgcg agagatggga ttctgttcag
    13501 gaattgaaaa ctattccgta catttaactt tgcgaccact gggttcgaca ccatatactt
    13561 tattggatta ctttggcgat gattggttag taatgattga tgaatcacat gtgacattac
    13621 cgcaagttcg aggcatgtat aacggagaca gagcgcgtaa acaagttttg gtggatcatg
    13681 ggtttagatt accgagtgca ttagataacc gtccacttaa atttgaagaa tttgaagaaa
    13741 agacaaaaca acttgtgtat gtatctgcaa cgcctggacc atacgaaatt gaacatacgg
    13801 ataagatggt tgaacaaatt attcgtccta ctggtttact ggatcctaag attgaggtta
    13861 gacctactga aaatcaaatt gacgatttat taagtgaaat tcaaacaaga gttgagcgta
    13921 atgaacgcgt acttgttaca acgctcacta aaaagatgag tgaagattta accacataca
    13981 tgaaagaagc gggtattaaa gttaattatc tgcattcaga aatcaagaca ttagaacgaa
    14041 ttgaaataat tagagactta cgaatgggta catatgatgt tatcgtaggt attaatttat
    14101 taagagaggg tattgatata ccagaagttt ctctagttgt catattagat gcagataaag
    14161 aagggttttt acgttctaac cgctcattaa ttcaaacaat aggtagagct gcgcgtaacg
    14221 ataaaggtga agtcattatg tatgccgata aaatgactga ttcgatgaag tatgcaattg
    14281 atgagacaca acgtcgtcga gaaatacaga tgaaacataa tgaaaaacat ggtattacac
    14341 ctaaaacaat taataaaaaa atacatgatt taattagtgc tactgttgaa aatgacgaaa
    14401 ataatgacaa agcacaaact gtgataccta agaagatgac gaaaaaagaa cgtcaaaaga
    14461 caatcgacaa tatagaaaaa gaaatgaaac aagcagcgaa agatttagat ttcgagaaag
    14521 ctacagaatt aagagatatg ttatttgaat taaaagcaga agggtgacaa gtaaatgaaa
    14581 gaaccatcca tagtagtaaa aggtgctcgt gcgcataact tgaaagatat tgatatcgaa
    14641 ctacctaaaa ataaattaat tgttatgaca ggtttatctg ggtcaggtaa atcgtcatta
    14701 gcattcgata ctatatatgc tgaaggacaa cgacgttatg ttgaatcatt aagtgcctat
    14761 gcgcgtcaat ttttaggcca aatggacaaa ccagatgttg atacaattga aggattatcg
    14821 ccagcaattt caatagatca aaaaacaaca agtaaaaatc caagatcaac tgtagcaaca
    14881 gtaacagaaa tatatgatta tatacgtttg ttatatgcac gtgttggtaa accttactgt
    14941 ccaaatcaca atatagaaat tgaatcgcaa acagtacaac aaatggttga ccgcattatg
    15001 gaattagagg cacgtacaaa gattcaatta ttagcacctg tcatcgctca tcgtaaaggt
    15061 agtcatgaaa agctaatcga agatattggt aaaaaaggtt atgtacgttt aagaatcgat
    15121 ggcgaaattg ttgatgtaaa tgatgtacct actttagata agaacaagaa tcatacaata
    15181 gaagttgttg tagaccgatt agttgttaaa gatggaattg aaacacgact agctgactct
    15241 atagaaactg ccttagagct ttcagaagga caattaacag tcgatgtcat tgacggggaa
    15301 gaccttaagt tttcagaaag ccatgcttgt cctatatgtg gattttcaat cggagagtta
    15361 gaaccaagaa tgtttagctt taacagtcct tttggtgctt gtccgacatg tgatggctta
    15421 ggccaaaagt taacagtcga tgtagacttg gttgttcccg acaaagataa gacgctaaac
    15481 gaaggtgcaa tagaaccttg gataccgacg agttctgatt tttatccaac attgttaaaa
    15541 cgtgtttgtg aagtttataa aatcaatatg gataaacctt ttaaaaagtt aacagaacgt
    15601 caacgtgata ttttattgta tggttctggt gacaaagaaa ttgaatttac atttacacaa
    15661 cgtcaaggtg gtactagaaa acgaacaatg gttttcgagg gtgtagttcc taatataagt
    15721 agacgattcc atgaatctcc ttcagaatat acacgtgaaa tgatgagcaa atatatgact
    15781 gaactacctt gcgaaacttg tcatggaaag cgattgagtc gtgaagcgtt atctgtttat
    15841 gtaggtggtt taaatattgg tgaagtagtc gaatattcaa tcagtcaagc gctgaactat
    15901 tataaaaaca ttgatttgtc agaacatgat caagcgattg caaatcaaat attgaaagaa
    15961 attatttccc gactcacttt tttaaataat gtgggacttg aatatttaac gttaaacaga
    16021 gcttcaggta cactttcagg tggtgaagca caacgtattc gattagcaac gcaaattggg
    16081 tcgcgtttga ctggtgtctt atatgtatta gatgagccat caattggact gcatcaaaga
    16141 gataatgatc gattaattaa tacacttaaa gaaatgagag atttaggaaa tactttaatt
    16201 gtagttgaac acgatgatga tacaatgcgt gcggctgatt acttagtgga tataggtcct
    16261 ggtgctggtg aacatggagg gcagattgtg tctagtggta ctcctcaaaa ggtaatgaaa
    16321 gataaaaaat cattaacagg acaatacttg agtggtaaga aacgtattga agtacctgaa
    16381 tatcgcagac cggcttcaga tcgtaaaatt tctatacgtg gagctagaag caacaatctt
    16441 aaaggggttg atgtggacat accactatca atcatgacgg ttgttacagg tgtatcaggt
    16501 tctggtaaaa gctcattagt aaatgaagta ttatacaaat cattagctca aaaaattaat
    16561 aaatctaaag taaagccagg attgtacgat aagattgaag gtattgatca acttgataaa
    16621 attattgata ttgatcaatc accaataggt agaacgccac gctctaatcc agcaacatat
    16681 actggtgtgt ttgatgatat acgtgatgtg tttgcgcaaa caaatgaagc taaaattcga
    16741 ggatatcaaa aagggcgttt tagttttaat gtaaaaggtg gacgctgtga agcttgtaaa
    16801 ggtgacggta ttattaaaat tgaaatgcat tttttacctg atgtttatgt tccttgtgaa
    16861 gtgtgtgatg gtaaacgata taatcgtgag acactagagg ttacttacaa aggtaaaaat
    16921 attgctgaca ttttagaaat gactgttgaa gaagcaacac aattttttga aaatattcct
    16981 aagattaagc gcaagttaca aacactagtt gatgttggtc ttggatacgt cacattaggt
    17041 caacaagcta caacgttatc aggtggtgag gctcaacgtg tgaaacttgc atctgaactt
    17101 cataaacgtt caactggtaa atctatttat atcctagatg aaccgacaac agggttacat
    17161 gttgacgata ttagtagatt attaaaagta ttaaaccgat tagttgaaaa tggtgatact
    17221 gttgtaatta ttgaacataa cctagatgtt atcaaaacag cagactatat tatagactta
    17281 ggtcctgaag gtggtagtgg cggtggtact attgttgcga ctggcacacc cgaagatatt
    17341 gctcagacaa agtcatcata tacaggaaag tatttaaaag aagtacttga acgagataaa
    17401 caaaatactg aagataaata agattaaaag aagtgaagga tgttataaat ttatccttcg
    17461 cttcttttta ttaatttagt aatgaatagt agaaagaaaa gatgcgtaaa aagaattatg
    17521 ttaagatagg gtcaatctag agtagttaaa cataaatcga actgggagtg ggacagaaat
    17581 gataaagaat cactaatgat ttattatgta gtggttcttt gtcattagcc acagctattg
    17641 tgtacttaaa aataggaatg catgagtgca actcatgcat aagaaatact aatttctaaa
    17701 gaaaaagtat ttctttatgt tggggccccg ccaacttgca ttgtttgtag aatttctttt
    17761 cgaaattctt tatgttgggg ccccgccaac ttgcattgtt tgtagaattt cttttcgaaa
    17821 ttctttatgt tggggccccg ccaactaatt ccaatatatc attgtagagc ttaggtcatt
    17881 gatttttggc tcggactttt atggcgatat gaaccatgta aattaagcaa gcaataaatt
    17941 aatgattgat attgacttgt aaaataataa caataatgaa caattaatat ttattttagc
    18001 ttttcaatgt agattggtgt tatatttttg atatgataag aagagatgta agagtaggga
    18061 taaatacaat tgaggtgaac ccatgttaac gacagaaaaa ctagttgaaa cattaaagtt
    18121 agatttaatc gctggtgaag aaggactatc gaagccaatt aaaaatgctg atatatcaag
    18181 accgggctta gagatggcag gttatttttc acattatgcg tcagatagaa tacaactatt
    18241 aggaacaacg gaactatcgt tttacaattt attaccagat aaggatcgcg caggtcgtat
    18301 gcgtaaacta tgcagaccag aaacgcctgc aattattgtg acacgtggat tgcagccacc
    18361 agaagaatta gttgaagctg caaaagaatt aaatacccca cttatagttg ctaaagatgc
    18421 gactacaagt ttaatgagtc gcttaacaac gtttttagag catgcacttg caaagacgac
    18481 atctttacat ggtgttttag tagatgttta cggtgttggt gtactaatta ccggtgattc
    18541 aggaataggt aaaagtgaga ctgcgttgga attagttaaa cgtgggcata gattagtagc
    18601 agatgataat gtagaaatac gtcaaattaa taaagatgaa ctaataggga aaccaccaaa
    18661 gttaatagaa catctattag aaatacgtgg actaggtatt atcaatgtta tgactttatt
    18721 tggcgcgggt tcaatattaa ctgaaaaacg aattagatta aatattaatt tggaaaactg
    18781 gaacaagcaa aagttatatg accgcgtagg tcttaatgaa gagacgctaa gtattttaga
    18841 tactgaaatc actaaaaaaa caatacctgt aagacctggt agaaatgttg cggtaattat
    18901 tgaggtcgct gcaatgaact atcgattaaa tatcatgggc attaacacgg ccgaagaatt
    18961 tagtgaaaga ttaaatgaag aaattatcaa gaacagtcat aagagtgagg agtaggttga
    19021 atgggtattg tatttaacta tatagatcct gtggcattta acttaggacc actgagtgta
    19081 cgatggtatg gaattatcat tgctgtcgga atattacttg gttactttgt tgcacaacgt
    19141 gcactagtta aagcaggatt acataaagat actttagtag atattatttt ttatagtgca
    19201 ctatttggat ttatcgcggc acgaatctat tttgtgattt tccaatggcc atattacgcg
    19261 gaaaatccaa gtgaaattat taaaatatgg catggtggaa tagcaataca tggtggttta
    19321 ataggtggct ttattgctgg tgttattgta tgtaaagtga aaaatttaaa cccatttcaa
    19381 attggtgata tcgttgcgcc aagtataatt ttagcgcaag gaattggacg ctggggtaac
    19441 tttatgaatc acgaggcaca tggtggatcg gtgtcacgcg cttttttaga acaattacat
    19501 ttgcctaatt ttataataga aaatatgtat attaacggcc aatattatca tccaacattc
    19561 ttatatgaat ccatttggga tgtcgctgga tttattatct tagttaatat tcgtaaacat
    19621 ttaaaattag gagaaacatt ctttttatat ttaacttggt attcaattgg tcgattcttt
    19681 atagaaggat tacgtacaga tagcttaatg ctcacaagta atattagagt tgcacaatta
    19741 gtatcaattc ttttaatttt aataagtata agtttaattg tatatagaag gattaagtat
    19801 aatccaccgt tgtatagcaa agttggggcg cttccatggc caacaaaaaa agtgaagtag
    19861 tgatagtttg aggaaatttt tatcaaaaac acatcatcat acaaaccctt tatggcgtgt
    19921 ataccgtctt gttaaatttt cgaaagtttt taagaatgta attatcattg aattttcgaa
    19981 attt
//
LOCUS       DAKTLH010000034        19712 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.034, whole genome shotgun sequence.
ACCESSION   DAKTLH010000034 DAKTLH010000000
VERSION     DAKTLH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19712
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.034"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(139..2115)
                     /gene="sfaD"
                     /locus_tag="O0394_001055"
                     /gene_synonym="sfnaD"
     CDS             complement(139..2115)
                     /gene="sfaD"
                     /locus_tag="O0394_001055"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HCY0499144.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPK
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPLIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSDFESSAKSWVETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            2217..3410
                     /gene="sfaA"
                     /locus_tag="O0394_001056"
     CDS             2217..3410
                     /gene="sfaA"
                     /locus_tag="O0394_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HCY0499145.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAVLLCLPLHFKVTKIPEDASRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPKVLKYIGMVNM
                     YYLSSFLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            3397..5154
                     /gene="sfaB"
                     /locus_tag="O0394_001057"
     CDS             3397..5154
                     /gene="sfaB"
                     /locus_tag="O0394_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HCY0499146.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            5161..6231
                     /gene="sfaC"
                     /locus_tag="O0394_001058"
     CDS             5161..6231
                     /gene="sfaC"
                     /locus_tag="O0394_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HCY0499147.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNHY"
     gene            complement(6332..6457)
                     /locus_tag="O0394_001059"
     CDS             complement(6332..6457)
                     /locus_tag="O0394_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499148.1"
                     /translation="MYHCYIIFALIQLTPFSMTVIPVIQLILSVAILCDELLHLI"
     gene            6623..7606
                     /locus_tag="O0394_001060"
     CDS             6623..7606
                     /locus_tag="O0394_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0499149.1"
                     /translation="MRGLKTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTI
                     AKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            7618..8649
                     /locus_tag="O0394_001061"
     CDS             7618..8649
                     /locus_tag="O0394_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0499150.1"
                     /translation="MAIKEISSQSAIDHKRKRRTTLTYIVSLCFLFICIYLNMAIGSS
                     KINFSDIIHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFFIVFVTIIIPALEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWIIGALIV
                     TIFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            8646..9614
                     /locus_tag="O0394_001062"
     CDS             8646..9614
                     /locus_tag="O0394_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCY0499151.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MTFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            9850..11220
                     /locus_tag="O0394_001063"
     CDS             9850..11220
                     /locus_tag="O0394_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCY0499152.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(11352..11612)
                     /locus_tag="O0394_001064"
     CDS             complement(11352..11612)
                     /locus_tag="O0394_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499153.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            11789..12304
                     /locus_tag="O0394_001065"
     CDS             11789..12304
                     /locus_tag="O0394_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCY0499154.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPKGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            12348..13535
                     /locus_tag="O0394_001066"
     CDS             12348..13535
                     /locus_tag="O0394_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HCY0499155.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVRVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            13554..14237
                     /locus_tag="O0394_001067"
     CDS             13554..14237
                     /locus_tag="O0394_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCY0499156.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
     gene            14414..15757
                     /locus_tag="O0394_001068"
     CDS             14414..15757
                     /locus_tag="O0394_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0499157.1"
                     /translation="MRLKSIITVIALILIMFMSAIESSIISLALPTIKQDLNAGNLIS
                     LIFTAYFIALVIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRV
                     IQGFGSGVLMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWH
                     WLFYINIPIAIIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQL
                     LLLNFLSFILAIVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNL
                     YIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVS
                     SIIISFGIKLPVLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNL
                     GASIGSTIMGYLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE"
     gene            15856..16329
                     /gene="sepA"
                     /locus_tag="O0394_001069"
     CDS             15856..16329
                     /gene="sepA"
                     /locus_tag="O0394_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HCY0499158.1"
                     /translation="MIVNYLKHKFYNLLTTMIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
     gene            16651..18093
                     /gene="lmrS"
                     /locus_tag="O0394_001070"
     CDS             16651..18093
                     /gene="lmrS"
                     /locus_tag="O0394_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HCY0499159.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTATGLI
                     IDQASWRVPFIIILGIAILAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALILSMVIIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            18241..19305
                     /locus_tag="O0394_001071"
     CDS             18241..19305
                     /locus_tag="O0394_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HCY0499160.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            19394..19579
                     /locus_tag="O0394_001072"
     CDS             19394..19579
                     /locus_tag="O0394_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499161.1"
                     /translation="MLSNKRVEKEAGCEHKKFLKKLLTLNSKVDNMIKSSKTTQLFLK
                     KNFKSVKLTIEITIKTI"
BASE COUNT         6527 a         2681 c         3616 g         6888 t
ORIGIN      
        1 agggatttat gtcccagcct gttttttgtg atttttaata atttgaatat ggaaaatgta
       61 ttattctctc atttgtatag attgtattta ataagttaat gtaatccttg agctcacgat
      121 taataaaatt ctataacctt aattattttc tcgatacaaa gggttattaa ctttaatata
      181 agtataatga tgtgcctcat cttcaagacg cattgttgta acacacttat aatctataaa
      241 tggcgcgaac atggtatctt taatttcatt tatgcgattc tcatttactt gattagattt
      301 gtgtgtcgaa agtacaagtt gatcaaaaat gttatctagt acatcacgaa cgatatacca
      361 catatgtctt tctaagtttg aatcatttga tgctttagag attgtaagaa ttaattcgcc
      421 taaatggttt tgaacggtag aataaaaggc tttgttaaac acagacgttt ttgaatcagt
      481 aagaattctt gatttttcat ggaaatgaga tgtactgtat cccatttcat ttagttgtgc
      541 tttatcaatt cttaaacctt caaagtctct aatatacatc gtgtccaata gaccatcttt
      601 ccgaaatgtt gcaatagcat tttgcaagtg agcctctaat gcaatgccat atttagtaac
      661 tagtgggatt acgagaccaa gcaatgcttt actataagtt tcaacccacg atttcgccga
      721 tgattcaaaa tcagacaatg atgcagctga ttgataacgt ttaatcaatg tcacgatagg
      781 tgattcattg ttaaatgggt aggttgcaac taagcttgaa ggaatcaatg gtgtgacttc
      841 ttgtggaatc atttggtata tattttttct aaataatgta cctaattgtt cacttcgttc
      901 agtttgatag tctgcttcgt cttgctcatt ataaaaatga ataccagcaa cttcatcaat
      961 aattgttgat gcataggact taaatatgac atctttctcc aaaatatcat ttaaaatacg
     1021 tgtcattagt ggaccattgt gcgtcgtttg ttctgataat gtacgaatct cacctgtaat
     1081 atgaacgttt gtcgacaatt tgatgtgtgg cgacatagct gggtatttag gaactaatgt
     1141 tctgaaagat aaaccagcat aataatccaa cgtatgtttt gcttcaatga ttaattcttt
     1201 atctacttct gcttgataat cagaatgtaa tacgtcatct aattgccatg gatgaacaat
     1261 cataatgtga taatcattaa agttaaactt tggcgtaaat tcattttcta attgtttaat
     1321 taagtccgga aatagttgat gaacagttgt atcataatct ttagacagtg acatagtacg
     1381 gcttaattta ctgtgaatca gtactatttt caacttaata ggttgattga attctgaaga
     1441 atataggaat gtttgtaatg catttaaacc tttacgtaat ttagccccag gatgtagcgg
     1501 atgaccttca ataacggctt gctctgaacg caagtaacta tcttcgctat tttcgataat
     1561 attaaataaa ggtgcagaat catgttgcat tgacagtgct tgatagctaa ttgcaaatgt
     1621 catattagtt gcactgttta ttaaatcttg ctgaaattga tcactagcag cattttttaa
     1681 atctggtgct tcaattaaaa tacactcaag aatttcattt ggatggtgta ctcgtgtaat
     1741 cgtatttgta atgtcatctt taatgtagaa agggccttca acatcaattc gatcaaaggc
     1801 gtgttctcca gtgataggag catataatgt ttgcttagct tgtgggaact ggatttctag
     1861 tatatgagtc gttgagatat ctaacataat caaatcacga ctcaatattt tcttactttg
     1921 agtgcgcgct ttaactaagt tttcgcgatg cattgatgtg accaatctct gagtgacttt
     1981 atctcttcct tgtaaaatca tctctttaaa aatattagcc caatcgctat tatgttgttg
     2041 taaaaataaa tatgtttctt gttcttcttt gttaaatttt aatgtctgtt ctttaaaaat
     2101 taagttcaag ttcataattc acctctatga aatattttac aaaagcaaga tagatttgta
     2161 taatccatat taatgataat gattcttatt atcaacagaa tgcgggtgta agttttatga
     2221 caaaatattt ttttagcagt tcttttctac tatttctagg taattggatt ggacaaatag
     2281 ggctaaattg gtttgtactt accacttatc ataacgcagt ttatctgggg attgtcaatt
     2341 tttgcagact tgtaccaata ttattactaa gtgtgtgggc aggggcaatt gccgataaat
     2401 atgataaagg gcgattgctg agaattacaa tttcatcatc atttttagta actgcaattt
     2461 tatgtgtgct cacgtatagt ttcactgcaa ttccaattag cgtcattatt atatatgcga
     2521 cattaagagg gattttaagt gcggttgaaa cacctttaag acaagcaatc ttaccagatt
     2581 tatcagataa aatatctact acacaagctg tatcatttca ttcattcatc attaatattt
     2641 gtcgttcaat agggcctgcc attgctggtg tcatattagc agtctatcat gcgccaacaa
     2701 catttcttgc acaggcaatt tgttatttta tcgcagtttt attatgctta ccattacatt
     2761 ttaaagtaac taaaatacct gaagatgcat caagatacat gccgttaaaa gttattatag
     2821 attacttcaa attacatatg gaaggtcgac aaatatttat aacatcatta ttgattatgg
     2881 cgacaggttt ttcatatacg acacttttac cagttttgac aaacaaagta tttccgggga
     2941 aatctgaaat atttggtatc gctatgacga tgtgtgccat tggtggtatt attgcaacgc
     3001 tagttttacc taaagtactt aaatatattg gtatggtaaa tatgtattat ttaagttcat
     3061 ttttatttgg cattgctttg ttaggtgtgg tatttcacaa tattgtcatc atgttcattt
     3121 gtattacatt gattgggtta tttagtcaat gggcacgtac gacaaatcgc gtttattttc
     3181 aaaataatgt taaagattat gaacgtggta aagtactgag tattattatg atggatagag
     3241 gtatgattcc attgggaagt ctattaatga gtatatgtgc agatgtgttt ggcattgtta
     3301 gaactttttc aataatggga ataagtacta tatgcattac aatggtattc tattttataa
     3361 atagaaagtt gaagttaaag ttggaggaaa gtaatcatgg tatatcttga atgggcaaag
     3421 gcagatagaa atattcaata tcgtgtaatt aacgccatta ttaaagaacg tatttacccc
     3481 gagcaaacat ttatttcgca aaaaggatct ttaattgaaa ttcagtatca tatgcatgtg
     3541 ttgactattg aagttgttag aaaaagtgca ttagaacgct atgagtttac aggtgatatt
     3601 acttatttaa ataaaggtga aacgtcatta attataactt tagaaggttt attagatgtg
     3661 ttgaatcatg actttgatat ccctatttca gagcgactac gcgaagagtt aatacacagt
     3721 cgagatagtt tagttgaaac atataagcaa atgtctcaca gacaaacgtt aataagtcaa
     3781 agttttaaat tttcaaggtt accacaagat attaactttt tttcatggtt acaacatgta
     3841 aaagatagtg ataagacaga tgatttaact tattctgaga gtttggtacc agaggggcat
     3901 ccaacacacc ctttaaccaa aacgaaattg cccttaacta tggaagaagt acgagcatat
     3961 gcacctgagt ttgaaaaaga aatccctttg caaattatga tgattgaaaa agaccatgtt
     4021 gtgtgcacag ctatggatgg taatgatcaa tttattattg atgaaataat tcccgaatac
     4081 tacaatcaga ttcgtgtgtt tttaaagagt ttaggtttga aaagtgaaga ctatagagcg
     4141 attttagtac atccttggca atatgatcat acgataggga aatattttga agcatggatt
     4201 gctaaaaaaa tattaattcc aacgccgttt acaatacttt caaaagcaac tttatcattt
     4261 aggacgatgt ctttaattga taaaccatac catgttaagt tgcccgtcga tgcacaagca
     4321 acaagtgccg ttagaacagt ctcaactgtg actactgtag atggaccaaa gttaagttat
     4381 gctttacaaa acatgttgaa tcaatatcca ggatttaaag ttgctatgga accgttcggt
     4441 gaatatgcaa atgttgataa agatagggca cgtcagcttg cttgtattat aagacaaaag
     4501 cctgaaattg atggaaaagg tgcaacagta gttagtgcaa gtctagttaa taaaaatcca
     4561 atagatcaaa aagttatcgt ggatagttac ttagagtggt taaatcaagg aattactaaa
     4621 gaaagtatta cgacatttat tgaacgatac gctcaagcat taatcccgcc tttaattgct
     4681 tttattcaaa attatggaat tgctttagaa gcacacatgc aaaatacagt agtgaacttg
     4741 gggccacatt ttgacattca atttttagtg agagatttag gtggttctag aattgattta
     4801 gaaacattac aacatcgtgt atcagatatt aaaattacaa atgatagttt aatagctgat
     4861 tctatagatg cagtgattgc aaaattccaa catgctgtta ttcaaaatca aatggcagaa
     4921 ttaatccatc attttaatca gtatgattgt gttgaagaaa ccgaattatt taacatagta
     4981 cagcaagtag tagcgcatgc cattaaccca acactaccac atgcaaatga gttaaaagat
     5041 attttgtttg gaccaacaat tactgtcaaa gcgttgttaa atatgagaat ggaaaataaa
     5101 gtaaagcaat atttaaatat tgagttagat aatccgataa aaaaagaggt gtagtactac
     5161 atggcacacg ttaacataaa tatatcgaag attaaatata acgccaaagt acttcaaaca
     5221 gtttttcaaa gtaaaaatat gcaattcaca ccagtaatta agtgcatagc tggtgaccgt
     5281 acaattgtag aaagcttaaa agcgttaggt atcaatcatg ttgcagaatc cagattggat
     5341 aacataatta gtattgcaga tcaggattta acatatacgt tattgcgtac gcctgccaaa
     5401 aaagagattt cagatatgat agaaaaagtt gatatgagta tccaaacaga attatctacc
     5461 atacatcaaa taaatgaagt agcagaagtt ttaggaaaaa aacataagat tttattaatg
     5521 gtagattgga aagatggccg tgaaggtgtt ctgacttatg atgttttaga ttatattaaa
     5581 gagattattc acttgaaaaa tatccatttt gtaggtttag cattcaattt tatgtgtttt
     5641 aaatctgatg cgccaagtga tgatgatatc tttatgatta atcgattcgt ctcagctgtt
     5701 gaaagagaaa taggatatcg attgaaaatt atttcaggtg gtaattcgag catgttacca
     5761 caacttttat ataatgattt gggtaaaata aatgaattgc gaataggtga aacgttgttt
     5821 agaggtgttg atacaacgac aaatcaagcg atcgctatgt tatatcaaga tgcgattaca
     5881 ttggaggctg agattttaga aattaaacct cgagttaata cacaaacgca tgaatcattc
     5941 ttacaagcta ttgtagatat tggttattta gatacaaaag tcgataatat ttcacctatg
     6001 gatcagcata tcaatatttt aggcgcttcg agtgatcatt taatgcttga tttaaatggt
     6061 caaggacact atcaagttgg agatcatata tctttttcat taaattatga agcactttct
     6121 cattcgatgt atatgaaaaa tttacataag gtatatattg atgattcaaa gattgatacg
     6181 ctacttcaaa attttgatgt gaaatctcct gctatggtaa accactatta atatatttat
     6241 caataagtct aagttgacaa gttatactac atatcgtttg cacggttgta tcataattgt
     6301 tcaacttaga ttttttgtat ttgttgattt atcaaattaa gtgcaacagt tcgtcacata
     6361 aaattgcaac agataatatc agctgaatta cagggataac ggtcatgcta aatggtgtca
     6421 attgtattaa tgcaaagatg atatagcaat gatacattat cagtattttg tctaaggaaa
     6481 tgtgctaatt gtagtcataa ttattagagg aaatatatag tcatacattt tagaaataca
     6541 aaaaagattg aacgctactt gacaatgata attgttatca ataaaataat aaatgaagtt
     6601 atacatatta aggagtggaa cgatgagagg tctaaaaact tttagtatat tgggattaat
     6661 agttgcctta cttttagttg cagcttgtgg taatacggat aattcaagta aaaaagaatc
     6721 atcaactaaa gatactattt cggtaaaaga tgaaaatggt acagtaaaag tacctaaaga
     6781 tgcaaaacgt atcgttgtat tagagtactc atttgcagat gcattagcag cattagacgt
     6841 taaaccagtt ggtattgctg atgatggtaa gaaaaaacgt atcattaaac cagttagaga
     6901 aaaaattggg gattatactt ctgtaggtac acgtaaacag ccaaacttag aagaaattag
     6961 taaattaaaa ccggatttaa ttatcgctga tagcagtaga cataaaggta ttaataaaga
     7021 attaaacaaa attgcaccaa cattatcatt aaagagtttt gatggagact acaaacaaaa
     7081 tattaattcg ttcaaaacaa ttgctaaagc tttaaataaa gaaaaagaag gcgaaaaacg
     7141 tcttgctgag catgataaat taatcaataa gtataaagat gaaattaaat ttgatagaaa
     7201 tcaaaaagtg cttccagcag tagttgctaa agctggttta ttagcacatc caaactattc
     7261 atatgttgga caatttttaa acgaactagg atttaaaaat gcattaagtg acgatgtaac
     7321 aaaaggttta agtaaatatt tgaaaggacc ttacttacaa ttagacactg aacatttagc
     7381 tgatttaaat ccagagcgta tgatcattat gacagatcat gctaaaaaag attctgctga
     7441 attcaagaag ttacaagaag atgcaacatg gaaaaagttg aatgcagtta aaaataatcg
     7501 cgtggatatt gttgaccgtg atgtttgggc aagatctcgt ggcttaattt cttctgaaga
     7561 aatggctaaa gaacttgttg aattatcaaa aaaagaacaa aagtaaggtg gaagtaaatg
     7621 gctataaaag aaataagtag ccaatctgcc atagatcata aaagaaaaag acgcacaaca
     7681 ctcacgtata tagtgagttt gtgctttctt tttatttgta tatatttaaa tatggcgatt
     7741 ggttcttcga aaattaattt tagcgatatc attcactatg ttactggtca tacagatacg
     7801 aaagcaacgt ttttattgca taatgtacgt atgccaagga tgattgcagg gttatttatt
     7861 ggcggtgcat tagcggtatc tggtttgtta atgcaagcaa tgacacgcaa tccgctagca
     7921 tcacctaaaa tttttggtgt cagttctggt gcatcgtttt tcattgtatt tgtgacgatt
     7981 atcattccag cattagaata ttatgcatta tatttaggtg tgataggtgc atttataggt
     8041 ggtttaactg tttatacact ttcaggtgct acgaaaggta tgacacccat taaattagca
     8101 ttggctggta tggccatcca tttgtttttt agtagtatga ccgaaggtat tattattttg
     8161 aatgagaatt caaacgaaca agtgatgttt tggcttgtcg gttcattatc cagtatgaag
     8221 tgggatgaaa ttttaaccat tctaccatgg attattggtg ctttaattgt aacgattttt
     8281 attggacgtc aactaacgat aatggaacta ggcgatgaca ttgctaaggg attgggtcaa
     8341 aatataaata aagttagaat tatcattgga ctattagtca ttattcttac aggtatgtcg
     8401 gtgtcagtgg caggaccaat tggatttgtt ggcttaatcg taccgcatat agtcaaacgc
     8461 tatgtgagta agaattattt agtcatgatt ccattaacat ttattatagg tgcagatttg
     8521 ttactactat cagatgtatt aagtcgttta attacatatc cttatgaatc accagtaggg
     8581 attgttacat cattcgtagg tgcactttac ttcttattta taacgattaa aggggtgaaa
     8641 agaatatgat gacaacgaaa attatacgtc gttatagttt aatcacaatc cttttaattg
     8701 tgagtatatt cataagttta tgtgtagggt ctgtcatgat tcatccgatt gatgctatta
     8761 agggaatctt tacacaagat gattttattt taaatgagta tcgtatacct agaacattac
     8821 ttggtattat tatcggtagt agcttggcta tttcaggtgc aattatacaa gctgtaataa
     8881 gaaacccgtt agcctcacct gatgttatag gtatttctaa aggtgccagt ttagctgctg
     8941 tgattattat tatgaccttt ccaacagcac cattgtttgt attacctata ggttcattct
     9001 taggtgcctt tgcagtaagt ttatttttat catttttaat ttcaaaatat gatgttaaag
     9061 gttcaaagct tgccttaatt ggtttagcaa taggcgcaat atgtacggcg attgtgcagt
     9121 tcttattaat cagaaatcca ctggaagcaa acaaagcgct tgtttggttg acaggtagtc
     9181 tatatggaca tgatatgtcg aatatttata ctatattacc ttggttttta ataacgatac
     9241 caatcatcat tttattgagt tatcaattag atgtattgaa tctaggtgat gctgttgcaa
     9301 ccgcactcgg tttaaaagtt aaaactatta aaatgctatt gttaatttta gcagtaatat
     9361 tagcaggatc cgcaatttca gttgtcggtg gtattagctt tttaggatta attgcacctc
     9421 atattgcgag acaattaata ggaaataaaa cattacacgt tattatcatg tcggggttaa
     9481 ttggtgctat attgttaaca tttggagatg gtttagcccg tggcattcat ccgccactgg
     9541 atattcctgt tggggttatc attgcaatta tcggtgcacc atacttctta atattattac
     9601 gaagaatgaa ataactaaca tatgattaga gtttaaaaaa ggttaagtac gaaatcagta
     9661 attgtactta accttttttg aattcatctt gaaaaattac aagaaatagc gtatatttaa
     9721 taactgtaat tattcattat tttgcataca taaacatttt gaaagataga atgataagga
     9781 atatgtttgt taactgaata gaattctgta tatacttaaa acgaatacat agagaaatgg
     9841 attgatttta tggatagctt ttcaaggaaa gagatagtta ttgggagact taagtttatt
     9901 acgatgtctt taattggtat cttattattt ttagtaccaa ttccagtcga acaagatgga
     9961 cagaagcaaa caacgttgcc cgtggcattt ttagcaggcg tgcttaaaga tgtattaggt
    10021 ggcgtaatgc catttttaat tgtgacgata attactttat cgggtatcat aactctgatt
    10081 tgttcaacta ttttaaaaga taaattgaaa cctgacgggc taatgaacaa tgcttttaac
    10141 gttagaattg gttggttaat tttacgaata ttagcagttg tttttgcatg gatgacattt
    10201 ttacgaattg gttcaaaggt tatttactca gatgaaacgg gtggattatt gttctcaagt
    10261 ttattgccaa cattagtggc agtattctta tttgcagctc tatttttacc gctattaatg
    10321 gaatacggtt tgttagagat gttgggtcct atttttagac cggttatgag acctttattt
    10381 acattgccag gacgttcgac ggtagataat ttagcatcat ttattggaga cggaactgtt
    10441 ggtgtcttaa ttacaagtag acaatatggt gaaggatatt attctcgtcg tgaggcaaca
    10501 gttatttcaa cgacatttag tgtagtttct atcacctttg caattgtagt tgctgaaaca
    10561 gtgcacatgc aaaatcaatt ttttgctttc tatttatcag tgatagtatc ctgcctcgtt
    10621 gcagcagtca ttatgccaag aatttggcct ttaaataaaa ttcctgatga gtatgctaag
    10681 gaggtacctg aaagtgcgcg tactgaggca ttgccagaag gaaagacggc attgagacat
    10741 ggctttgata ctgcaacaga agtaggtatt aaagcaccgg gcgttattga cttttttaag
    10801 tcaggattaa aaacagtcat tgatatgtgg tttgttattt tgcctgtagt aatgagtatt
    10861 ggtactattg ctaccattat tgcgaactac acgccctttt ttgtcatatt gggtaaacct
    10921 tttgttccat ttttagagtt aatgcaaata cctgaagcgg cacaggcttc tcaaacgatt
    10981 attatcggtt ttgccgatat gtttttacct tctatattaa ttgaaggtgt acaaaatgat
    11041 ataactcgtt ttgttattgg ggcacttagt atatcgcagt taatatactt gtctgaagta
    11101 ggtggcgtca tacttggttc gaagattcct gtaagcatcg gcaaattgtt tatgattttc
    11161 ttgattagaa caatcattac actaccaatt atttctttga tggcacatct tttgttataa
    11221 atatatgtga aaaaggacaa ctgttaaaat tatgggaaca gttgtccttt tgtatttgtt
    11281 tatgctgggt gttgaatcaa ttaaaaaaat cattcatgaa gccctcacca tgaatgattt
    11341 ttttatacaa tttatttagt taattcattg taaacttttt gatcattttg agtataaata
    11401 atgtattcgt ggtcttcagt atcgataata acgcgattcg ttttaccaaa cgttgagccg
    11461 attctagata catctttgct gtctaattta ggaactgcat gaatatcttg gtcttgtgta
    11521 atatttttaa tttcacttgt aggaattttg atatcagcaa ctctccattg aatacgcact
    11581 tcattattat ccttttttac tgtcattgcc attattcaaa cacatccttt aaatattttg
    11641 taaatacatc ataaacgatt ttgtaatcgt tttcaagagt tatgtgatac ttttttaaat
    11701 tgtgtcatac taatgaattt taacccgaaa atatgttcgt gtttttgtta gggatgtggt
    11761 aaaatgtgag tattaaagga gaacaaatat gacaggtaaa acacatgctt catgcggcat
    11821 gctcgttgga gcgctaacaa cacaatattt tcaaacggat attttttcgt cggttacggt
    11881 tatcattcta gcaactctag ctagcttatt acccgatata tgtcacactc aaagtaagat
    11941 tggcagaagg tttaaggtaa ttagtttttt tgtgagattg atctttggac atcgaacctt
    12001 tacgcattca atattattta tcgcaatcat agcattttta ttgcaaatta ttcagacgcc
    12061 taattattat atggcagcaa ttataattgg attagtgtca catgttatac tagatatgat
    12121 aacaccaaaa ggcgtgaaat tattttatcc gttaccgttt aatgtgaaga caccgattca
    12181 atttaaaaca ggtggacttg tagacttatc tttagctaca gcgttaatgg tcggaacagt
    12241 ttatgtttta tttcagcctt tcatcaatga tataataagc aactggaata ctaagttttt
    12301 atagaaaata aattattaaa ggttacaaac tcagcaggag gcataaaatg ctagataaaa
    12361 atcaattagc taaatataaa caagatcatt tgtgtgaata tgaaaaaata atgagtaaca
    12421 atgaaaaaga agcacttgaa gagaaggtag cgtcattaga tttagatttt attgccaaat
    12481 tatataacga tttatacatc aataagaaaa caatagatga tgtatctgca gtttctgaag
    12541 tgaaatatga tattaaatca caaatgagtg atgatgagat taagcgttta gaggaacaag
    12601 gacttcaagc tattaaagaa ggacaatttg cagtactttt gatggcaggt ggtcaaggaa
    12661 caagacttgg ttacaagggc cctaaaggtt cgtttgaaat tgaaggtgta agtttatttg
    12721 aacttcaagc taaccaatta aaaacattga atcatcaatc tggacatacg attcaatggt
    12781 atattatgac aagtgatatc aatcatgaag aaactttagc ttatttcgaa gcacatagtt
    12841 attttggata tgatcaagaa gcaatccatt tctttaaaca agataatatt gtggcgctta
    12901 gtgaagaagg gaagttaatt ttaaatcaac agggtcgtat tatggaaacg ccgaatggta
    12961 atggtggcgt gtttaaatct ttagacaagg caggatactt agaagaaatg tctaataatg
    13021 gcgttaagta tattttcttg aataatatcg acaatgtttt agtaagagtg ttggatccac
    13081 tatttgcagg atttacggta gaacatgatt acgatattac ttctaaaacg attcaaccaa
    13141 aaccaggaga aagtgttgga cgtttagtta atgtcgattg taaagataca gtactggagt
    13201 attcagaatt agatccagaa gttgcaaatc aatttaacaa tgctaatatc ggtattcatg
    13261 catttaaact tggttttatt ttaaacgctg taaatcgtga gttaccttat catttagcta
    13321 ttaagaattt gaagcaatta gatgagaact ttggtgttat tgaacaacca actttaaaat
    13381 tcgaattgtt ctattttgac atatttactt atggaactag ttttgtcacg ttacaagttc
    13441 ctagagaaga agaattttca cctcttaaaa ataaagaagg taaagatagt gttgcaactg
    13501 caacagaaga tttacgtaga atgggtttaa tttaatgata ggcggtgact atgatgtcca
    13561 aatcaactaa aaaaagtaat gacacaatcg tagaaacatt taaggatatc atccctttat
    13621 catttggaga agaaattggt aatgcggcat ctcatggtgt tgcagctttg ttaacattat
    13681 tagtgttacc ctatgcggct gtgcacagtt atattgcaca tggtacgctt gcgtctgtga
    13741 gtatgtctat ttatgttatt tctatcttta tgatgtttat ttcatctact atttatcatt
    13801 ctatgcaaaa tgaaacacct cataaatata ttttaaggat tatcgatcac agtatgattt
    13861 acgtagcaat ttctggtaca tatacgccta ttttgttaac agttgtaggt ggttggatag
    13921 gttggacagt atttatttta ttatggggaa cgacactttg ggggatttta tataaatcca
    13981 tagctgtcaa agttaaccaa aagttaagct taattgtcta tttagtgatg ggctgggtag
    14041 gtattatctt gttaccaatt atttttattc gcacatcatg gatattcata ttattcattt
    14101 tcttaggtgg tgtgtcttat acaattggtg catggtttta tgcacaaaag aatagaccat
    14161 attttcatat gatttggcat atttttattg taatagcatc actattgcat ttaattgcaa
    14221 tcttatattt tatgtagtcg tttagaaaag ctgggggaaa ctccgctttt tttattacat
    14281 aaatcattct aataggggat tgtattgtaa tagaattatc gttaagattt gaaagtcaaa
    14341 agtgtttata catatgaaaa ccaattattt gtaaataaaa tcttataaat aaatatatag
    14401 aaggaggtat ttcatgcgat taaagtcaat cataactgtt atagctttaa tattaattat
    14461 gtttatgtca gcgattgaat catctattat ttcgcttgca ttacctacaa ttaaacaaga
    14521 tttaaatgca ggaaatttaa tttcattaat tttcacagcg tattttatcg cattagtaat
    14581 tgccaatcct atagtgggcg aattactttc gagatttaaa attatttatg tggcaattgc
    14641 aggattactt ttatttagta taggtagttt tatgtgtggt ttaagtacta attttactat
    14701 gctaattata tcacgcgtga tacaaggttt tggatcaggt gtattgatgt cattatcgca
    14761 aattgttcca aagttagcat ttgaaattcc gttacgatat aaaattatgg gcatagttgg
    14821 cagtgtttgg ggtatttcga gtatcattgg gccacttcta ggtggtggta tattagaatt
    14881 tgcaacatgg cattggttat tctacataaa tataccgatt gcgatcattg ccattatatt
    14941 agttatatgg acatttcatt ttccagagga agaaacagtt gctaaatcaa aatttgatac
    15001 taaagggctc acgctatttt atgtatttat tggattgatt atgtttgctt tattaaacca
    15061 acagctgtta cttttaaatt tcttgagttt tatcttagct atcgttgtgg caatgtgttt
    15121 atttaaagta gaaaaacatg tttcttcacc atttttacct gtggttgaat ttaatcgttc
    15181 gattacttta gtttttataa ctgaccttct aacagctatt tgtttaatgg gattcaattt
    15241 atatattcca gtctaccttc aagaacaact aggattatct ccattgcaaa gtggattggt
    15301 tatttttcct ttatctgtag cttggattac attgaatttt aatttacatc gaattgaagc
    15361 aaaactatca aggaaagtta tttacttact atcatttaca ttgctactag taagtagtat
    15421 tatcatttca tttggtatta aattgccggt acttatagca tttgtgttaa ttttggcagg
    15481 attaagtttt ggatatattt atacgaaaga tagtgtgatt gtccaagagg aaactagccc
    15541 attacaaatg aagaaaatga tgtcatttta tggattaact aaaaatcttg gggcatcaat
    15601 aggttcaaca ataatgggat atctttatgc gatacaatca ggaatctttg gtccaaactt
    15661 acacaatgtg ttaagtgctg ttgctgtaat tagcattggg cttattgttt tatgggtcgt
    15721 attttttaaa gaacagtcat ctcaatcaaa agaatagaat tatgattaaa attgatgtag
    15781 tgtaattaaa gtaagaaata ataaatttcc tagatactag ataaatcgtt caatatttta
    15841 ttaataagga ggccaatgat tgtgaattat cttaaacata agttttataa cttgttaact
    15901 acaatgattg ttctctttat ttttgtactt tctggtgcga tttttttaac atttctaggg
    15961 tttggtttat atggattaag tagaatactt atttatttta ggttgggtga ctttacatat
    16021 aatagaagta tgtacgataa cctattatat tatggcagtt atataatctt tgggtacttc
    16081 atcatatttg cagtcgagca tttaatggat tattttagaa aaatgttgcc tgaaaatgca
    16141 tattttagag gcgcgacttt tcatttgatt tcttacacag ttgcaacaac gttattttat
    16201 tttattattc atctgaacta tgtgtatatc aatatcgatt tttgggtcat catggttatt
    16261 atagggttct tatatgtttg taagcttcaa ttttaccctg aaagtaaaaa tttaaataat
    16321 agaaaataga tatctgaagt tgagggcaca tatgtgttcc aacttctttt tgttcaatag
    16381 aagaatcgat aatagtgagt cttagtgctc aatttgtggt aaataagtta aataaccatt
    16441 gtatgagtct gagacataaa ataaattatt aggccctaca atgttatatt gttagttttt
    16501 aagtacacat tagctgtgcc taatgtgtaa gaaccaataa tgatttatta tgtagtgctt
    16561 ctttattatt tgagtactac tcccattatg ttcatggcta tacgtattaa agggacaagt
    16621 gtatatctgt tttaaaaagg aggaattatg atggctaaag ttgaattaac aacccggaga
    16681 cgaaacttca tagttgctgt tatgttgatt agtgcgtttg tagctatttt aaatcaaaca
    16741 ttattaaata cagcgttacc tagtataatg agagaattaa atatcaatga aagtacatcg
    16801 caatggctag ttactgggtt tatgcttgtt aatggcgtca tgatacctct gacggcatat
    16861 ctaatggata gaattaaaac tagaccttta tacttagcgg cgatggggac atttttatta
    16921 ggttctattg ttgcagcctt agctccgaat tttggagttt taatgttagc tcgtgtaatt
    16981 caagcgatgg gtgcaggcgt acttatgccc ttaatgcaat ttacgttatt tacattgttc
    17041 agtaaagaac atcgaggttt tgcaatggga ctagcaggtt tagtaattca atttgcacca
    17101 gcaataggac ctacagctac aggattaatt attgatcaag cgagttggcg agttccattt
    17161 attataattt taggaattgc tatacttgcc tttgttttcg gtttggtttc aatctcgagt
    17221 tacaatgaag tgaaatatac gaaattagat aagcgttcag taatgtattc aactattggg
    17281 ttcgggttaa tgctatacgc atttagtagt gcaggagatt taggatttac aagtccaata
    17341 gtaataggtg cgttgatatt aagtatggtt attatctatt tatttatacg tagacaattt
    17401 aatattacta atgcactttt aaatttaagg gtttttaaaa atagaacatt tgcattatgt
    17461 acgattagtt caatgattat aatgatgtca atggttggac ctgcgctgct tataccgcta
    17521 tatgttcaaa acagtttatc tttatctgcc ttgttatcag gacttgttat catgcctggt
    17581 gcaataataa atggtattat gtcagttttt acaggtaaat tttatgataa gtatggtcct
    17641 agaccattga tttatactgg ttttacaatt ttaacaatta ctacaattat gttgtgtttc
    17701 ttgcatacag acacatctta tacgtattta atagtcgtat atgcaattag aatgttttca
    17761 gtttctttac tcatgatgcc gataaatact acaggaatta attctttgag aaatgaagaa
    17821 atctcacatg gcacggctat tatgaacttt ggtcgtgtaa tggctggttc actaggcaca
    17881 gctttaatgg ttacattaat gagttttggt gcaaaaatat ttttatctac atcgccatcg
    17941 catttaactg caactgaaat taaacagcaa tccattgcta taggggtgga tatctcattt
    18001 gcttttgtag ctgtgcttgt tatggcagct tatgtgatag cactttttat aagagaacct
    18061 aaagaaatag aaagtaatag aaggaaattt taaaataatt atagtagttg gtctatttaa
    18121 aataataggc taactgcttt ttttatttaa taaaaagttt tatactttta gtgatagact
    18181 aagcaaaaat tgttatttgc tatgatgtag atgtcttaaa atgattaagg gggatttgct
    18241 ttgttaacgg tagatcaagt gaaagaattg gtaggagaaa ttaaagatcc tattatagat
    18301 gtgcctttaa aagaaacaga aggtattgtt gaagtttcta ttaaggaaga aaaagaacat
    18361 gtgagtgtta aattagcaat ggcacaatta ggtggtgcac cgcaattaga tttacagatg
    18421 gctgttgtta atgcattaaa agaaaacggt gcgaaaacgg tcggtatacg atttgaaaca
    18481 ttgccggaag aaaaagtaaa tcaatttaaa ccaaaagaag aaaataaacc taaaacgata
    18541 gaaggcctat tatctcaaaa taatccagtc gaatttattg caatagcctc cggtaaaggt
    18601 ggtgtcggta aatctactgt tgcagtaaat ttagccgttg ccttagctcg tgaagggaaa
    18661 aaagtcggat tagtagatgc cgatatatat ggatttagtg taccagatat gatgggtatt
    18721 gatgaaaagc ctggaattaa agggaaggaa gtaattccag ttgaacgtca tggcgttaaa
    18781 gttatatcaa tggccttttt tgtggaagaa aatgcgccag ttatatggag agggccaatg
    18841 ttaggtaaaa tgttgacgaa tttctttaca gaagttaaat ggggagacat tgaatattta
    18901 atactcgatc ttccacctgg aacaggagat gtagctttag atgttcatac gatgttacct
    18961 tcaagtaagg aaattattgt aacgacacct catcctacag cagcatttgt tgcagctcgc
    19021 gcaggtgcga tggcaaaaca tacggatcat tctattcttg gcgtaattga aaacatgagt
    19081 tattttgaaa gtaaagagac gggtaataaa gaatatgtct ttggcaaagg tggcggtact
    19141 aagttagctg atgaacttaa tactcaatta cttggggaat tacctttaga gcaaccatct
    19201 tggaatccaa aagattttgc accgtcaata tatcaatcag atgatcgtct aggtaaaatt
    19261 tatagctcga ttgcacaaaa agttatagct gctacaaata aataggtata atatatgcta
    19321 aacgtacaaa attactttta ttttgtacgt ttttatgttg atttgttttg gaatgctggt
    19381 aaaataagtt cttgtgttaa gcaataaacg cgttgaaaaa gaagcgggtt gtgaacacaa
    19441 aaagttttta aaaaagttat tgactttaaa ttccaaagtt gataatatga taaagtcgtc
    19501 aaaaacgacg cagttgtttt taaagaagaa tttcaaaagt gtaaaactta ccattgaaat
    19561 aactataaaa acaatataga ataaataaag taatcgttca aaaagttatt gacttcaaga
    19621 aataaatgaa gtagaataaa agagattgaa cattaatgaa cattgaaaac tgaatgacaa
    19681 tatgtcaacg ttaattccaa aaacgtaact at
//
LOCUS       DAKTLH010000035        19701 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.035, whole genome shotgun sequence.
ACCESSION   DAKTLH010000035 DAKTLH010000000
VERSION     DAKTLH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19701
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.035"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            118..1347
                     /locus_tag="O0394_001073"
     CDS             118..1347
                     /locus_tag="O0394_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCY0499162.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            1602..1694
                     /locus_tag="O0394_001074"
     CDS             1602..1694
                     /locus_tag="O0394_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499163.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
     gene            1872..2705
                     /locus_tag="O0394_001075"
     CDS             1872..2705
                     /locus_tag="O0394_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCY0499164.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            2987..3784
                     /locus_tag="O0394_001076"
     CDS             2987..3784
                     /locus_tag="O0394_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0499165.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            3820..4824
                     /locus_tag="O0394_001077"
     CDS             3820..4824
                     /locus_tag="O0394_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0499166.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            4821..5837
                     /locus_tag="O0394_001078"
     CDS             4821..5837
                     /locus_tag="O0394_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0499167.1"
                     /translation="MISSNNKRRQLIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDKLILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITTALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            6071..7039
                     /gene="dhaK"
                     /locus_tag="O0394_001079"
     CDS             6071..7039
                     /gene="dhaK"
                     /locus_tag="O0394_001079"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCY0499168.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            7081..7665
                     /gene="dhaL"
                     /locus_tag="O0394_001080"
     CDS             7081..7665
                     /gene="dhaL"
                     /locus_tag="O0394_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCY0499169.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLKNGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            7658..8020
                     /locus_tag="O0394_001081"
     CDS             7658..8020
                     /locus_tag="O0394_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCY0499170.1"
                     /translation="MPKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            8136..8633
                     /locus_tag="O0394_001082"
     CDS             8136..8633
                     /locus_tag="O0394_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499171.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            9089..10156
                     /locus_tag="O0394_001083"
     CDS             9089..10156
                     /locus_tag="O0394_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499172.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            10307..11350
                     /locus_tag="O0394_001084"
     CDS             10307..11350
                     /locus_tag="O0394_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0499173.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
                     DSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
     gene            11679..12107
                     /locus_tag="O0394_001085"
     CDS             11679..12107
                     /locus_tag="O0394_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499174.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
     gene            complement(12316..12822)
                     /locus_tag="O0394_001086"
     CDS             complement(12316..12822)
                     /locus_tag="O0394_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0499175.1"
                     /translation="MAHIIRRVSIKDVENFISMLANIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            12935..13858
                     /gene="graX"
                     /locus_tag="O0394_001087"
                     /gene_synonym="apsX"
     CDS             12935..13858
                     /gene="graX"
                     /locus_tag="O0394_001087"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HCY0499176.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFAISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            13874..14548
                     /gene="graR"
                     /locus_tag="O0394_001088"
                     /gene_synonym="apsR"
     CDS             13874..14548
                     /gene="graR"
                     /locus_tag="O0394_001088"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HCY0499177.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDQTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            14541..15581
                     /gene="graS"
                     /locus_tag="O0394_001089"
                     /gene_synonym="apsS"
     CDS             14541..15581
                     /gene="graS"
                     /locus_tag="O0394_001089"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HCY0499178.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNFLMLGISLIDYDFPIDSLF
                     YIVSLNLSLTMIFLLLTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
                     INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDD
                     YVYTDIKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEI
                     VERMSEVTNLSF"
     gene            15725..16486
                     /gene="vraF"
                     /locus_tag="O0394_001090"
     CDS             15725..16486
                     /gene="vraF"
                     /locus_tag="O0394_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HCY0499179.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGKKLEKLSNKELSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKEHMLNRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGVNYDL"
     gene            16476..18365
                     /gene="vraG"
                     /locus_tag="O0394_001091"
     CDS             16476..18365
                     /gene="vraG"
                     /locus_tag="O0394_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HCY0499180.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIY
                     ILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLI
                     GVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYL
                     SLNIVQYYDSIGTLMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSS
                     IMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQ
                     LASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGSIKDLVKHKKHGKAIIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLND
                     KDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKAKNKVDKSIETRSEAISSISSLTG
                     ILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIM
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRT
                     IRHSI"
     gene            19090..>19701
                     /locus_tag="O0394_001092"
     CDS             19090..>19701
                     /locus_tag="O0394_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HCY0499181.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKN"
BASE COUNT         6931 a         2587 c         3607 g         6576 t
ORIGIN      
        1 gtcataattc ttcgtatttt acatagcaat ttaactgatt ttagagtcca cggtacagaa
       61 gtttgatatt tcaatgtttc taaattttta aaaaattaaa tcataggtgg gtgccaaatg
      121 tttttattaa tcaacattat tggtctaatt gtatttcttg gtattgcggt attattttca
      181 agagatcgca aaaatatcca atggcaatca attgggatct tagttgtttt aaacctgttt
      241 ttagcatggt tctttattta ttttgattgg ggtcaaaaag cagtaagagg agcagccaat
      301 ggtatcgctt gggtagttca gtcagcgcat gctggtacag gttttgcatt tgcaagtttg
      361 acaaatgtta aaatgatgga tatggctgtt gcagccttat tcccaatatt attaatagtg
      421 ccattatttg atatcttaat gtactttaat attttaccga aaattattgg aggtattggt
      481 tggttactag ctaaagtaac aagacaacct aaattcgagt cattctttgg gatagaaatg
      541 atgttcttag gaaatactga agcattagcc gtatcaagtg agcaactaaa acgtatgaat
      601 gaaatgcgtg tattaacaat cgcaatgatg tcaatgagct ctgtatcggg agctattgta
      661 ggtgcgtatg tacaaatggt accaggagaa ctggtactaa cggcaattcc actaaatatc
      721 gttaacgcga ttattgtgtc atgcttgttg aatccagtaa gtgttgaaga gaaagaagat
      781 attatttaca gtcttaaaaa caatgaagtt gaacgtcaac cattcttctc attccttgga
      841 gattctgtat tagcagcagg taaattagta ttaatcatca tcgcatttgt tattagtttt
      901 gtagcgttag ctgatctatt tgatcgtttt atcaatttga ttacaggatt gatagcagga
      961 tggataggca taaaaggtag tttcggttta aaccaaattt taggtgtgtt tatgtatcca
     1021 tttgcgctat tactcggttt accttatgat gaagcgtggt tggtagcaca acaaatggct
     1081 aagaaaattg ttacaaatga atttgttgtt atgggtgaaa tttctaaaga tattgcatct
     1141 tatacaccac accatcgtgc ggttattaca acattcttaa tttcatttgc aaacttctca
     1201 acgattggta tgattatcgg tacattgaaa ggcattgttg ataaaaagac atcagacttt
     1261 gtatctaaat atgtacctat gatgctatta tcaggtatcc tagtttcatt attaacagca
     1321 gctttcgttg gtttatttgc atggtaatat gtcgaagagt gactatgata atacatttta
     1381 actaataaat atgtccaggc atgtcgtcta ttgatatagg tgagatgctt ggactttttt
     1441 attattgata taaaggtatt taaatatttt taaagttacc gaaattgaag cattataaaa
     1501 accaagtgca catggtaata cacttggctt ttatgggaaa tgaatattat tgtacatatg
     1561 acagtaagga ctaggtacag tcatagtact tcgagcaaaa tttgttttgt tattataaac
     1621 aacacaaagg agataacttc tctattgaag aagttaaaaa cattatagca gacaatgaaa
     1681 tgaaagtaaa ttaaaaattc agaatatttt taattattat attgtgagtg atatttatta
     1741 gggaaagcta ttcttcatat aaattagtta aatagtaaat ctttgttaga actctttcca
     1801 tagagttgtg acagcatttt tgttgtgcta ctatttttat agtctaataa atatataaag
     1861 gggatggttt cgtgaataaa acggttaaag atttaatact agttgtctta ggttcattta
     1921 tctttgctgc aggtgtaaat gcatttatta tttctggtaa cttaggtgaa ggcggggtta
     1981 caggtttagc aattatttta tattatgcgt ttcatatttc accagccatc actaacttct
     2041 tggtcaacgc agtattgatt gccataggtt ataaattttt gagtaagaga agtatgtact
     2101 taactattct tgtaacaatt cttatttcaa tatttttgag tttaacagaa tcatggcaag
     2161 tagaaactgg aaacagcatt gtgaatgcca tttttggtgg tgtaagcgtt ggactaggaa
     2221 tcggagtaat tatccttgca ggcggtacaa cagcaggtac aacaattttg gcgagaattg
     2281 caacgaaata cctcgatgta agcacgccat atgctttgct tttcttcgat atgatcgttg
     2341 ttgcaatttc acttacagtt attccacttg ataaagtatt agtaacagta atatcacttt
     2401 atataggaac aaaagtgatg gaatatgtca tagaaggttt aaacactaaa aaagctatga
     2461 cgattatttc aactaatccc gacaaacttg ccaaagcaat agacgagcaa attggaagag
     2521 gtttaaccat tttaaacgga catggctatt atacgcgtga agaaaaagat gtcttatacg
     2581 ttgttatttc taaaacacaa gtttcaaaag caaagcgatt aattaaacaa atcgataaag
     2641 atgcattcct cgtaattcat gatgtaagag atgtctatgg taatggcttt cttgcagatg
     2701 aataaataaa tggtatgagc acacatactt aaatagaagt ccacggacaa gtttttgaac
     2761 tatgaagact tatctgtggg cgttttttat tttataaaag taatatacaa gacatgacaa
     2821 atcgagctat ccaatttaaa aagtaatgtt agtcaataag attgaaaaat gttataatga
     2881 tgttcatgat aatcattatc aattgggatg tctttgaaaa ttgataattt aaaaatagaa
     2941 attatttttt ataaacagaa agaattttat tgaaagtagg gaaattatga atcgtttgca
     3001 tggacaacaa gttaaaattg gttacgggga taacacgatt ataaataaat tagatgttga
     3061 aataccagat ggcaaagtga cgtcaatcat tggtcctaac ggctgcggga aatctacttt
     3121 gctaaaggca ttgtcacgtt tattggcagt taaagaaggc gaagtatttt tagatggtga
     3181 aaatattcat acacaatcta cgaaagagat tgcaaaaaaa atagccattt tacctcaatc
     3241 acctgaagta gcagatggct taactgttgg ggaattagtt tcatatggtc gttttccaca
     3301 tcaaaaagga tttggtagat taactgctga ggataagaaa gaaattgatt gggcaatgga
     3361 agttacagga actgatacat tccgacaccg ttcaatcaat gatttaagtg gtggtcaaag
     3421 acaacgtgtt tggattgcaa tggcattagc acaaagaact gatattatct ttttagacga
     3481 accaacaaca tatttagata tctgtcatca attagaaata ctagaattag ttcagaagct
     3541 aaatcaggaa caaggttgta caattgtcat ggttcttcat gatatcaacc aagcgattcg
     3601 tttctcagat catcttattg cgatgaaaga aggggatatc atcgctacag gttcaacaga
     3661 agacgtatta acacaggaaa tattagaaaa agtttttaat attgatgttg ttttaagtaa
     3721 agatcctaaa actggaaaac ctttactggt aacttatgac ttatgtcgca gagcttattc
     3781 ttaattaagt aagttaatat gataaaaagg acaattaaca tgacaaatag agagaaccca
     3841 acgccattga agtttttatc ctatattata ggtttaagta tgatactact aatcacacta
     3901 tttatttcta cattaatagg tgacgccaaa attcaagcct ctacaattat agaggctatt
     3961 tttaattata atcctagcaa tcaacagcaa aacatcatca atgagattag gattcccaga
     4021 aatatagcag cagtaattgt aggtatggcg cttgcagttt ctggtgcgat tatacaaggt
     4081 gttactcgta atggtcttgc tgatccggcg ctcataggtt taaattcagg tgcttcattt
     4141 gctttagcat taacatatgc agttttacca aacacttcat ttttaatatt gatgtttgct
     4201 ggatttttag gtgctattct aggaggtgct attgtattaa tgataggccg atctagacgt
     4261 gatggattta atccgatgcg tattatttta gcgggtgcag cagtaagtgc tatgttaaca
     4321 gcgctaagtc aaggtattgc attagctttt agactaaatc aaacagtaac attttggact
     4381 gctggaggcg tttcaggcac aacatggtca caccttaagt gggcaattcc attaattggt
     4441 attgcgttat tcattatatt aacaattagt aaacaactta ccattttaaa tcttggtgaa
     4501 tcattagcta aaggtttagg tcaaaatgta acaatgatca gaggcatatg tttaattatt
     4561 gctatgattc tagcaggtat tgcagttgct atcgctggac aagttgcatt tgtaggtttg
     4621 atggtacctc atatagcaag atttttaatt ggaactgatt atgctaaaat tctaccatta
     4681 acagccttgt taggtgggat actcgtgctt gttgccgatg tgatagcacg atatttagga
     4741 gaagcgcctg ttggtgcaat catttcattt atcggtgttc cttacttttt atatttagtt
     4801 aaaaaaggag gacgctcaat atgattagtt caaataataa acgcagacaa ttgatagcac
     4861 tggctgtttt tagcattcta ctatttctag gttgtacttg gagtattacc tcaggtgaat
     4921 acaacatacc tgttgaaaga tttttcaaaa ctttaattgg acaaggtgat gccattgata
     4981 agttaatctt attagatttc aggttacctc ggatgatgat tactattttg gctggcgcag
     5041 cgcttagtat tagtggtgca atagtgcaaa gtgtcacaaa aaatccaata gctgaaccag
     5101 gtatattagg tattaacgca ggtggcggat ttgcaatcgc attatttatt gcaattggta
     5161 aaattaatgc tgacaacttt gtttatgtac tgccgttaat aagtatacta ggtggtatca
     5221 ccactgcatt gattattttt attttcagtt ttaataaaaa tgaaggtgtt acacctgcga
     5281 gtatggtatt aataggtgta ggtttacaaa cagcattata tggtggctca attacaatta
     5341 tgtcaaaatt tgatgataag caatctgatt tcatcgctgc ttggtttgca ggtaatattt
     5401 ggggtgacga atggccattt gtcattgcat ttttaccgtg ggtgttgatt attattcctt
     5461 acttactatt taaatcgaat acactaaata ttattcatac gggtgataat attgcacgag
     5521 gtctaggtgt aaggttaagc agagaacgtt taatattatt ctttatcgca gtgatgttat
     5581 catctgctgc tgtagcagta gcaggttcaa tttcgtttat cggattaatg ggtccgcata
     5641 ttgccaaacg tatcgttgga ccacgtcacc agttgttttt accaattgcc attttagtag
     5701 gggcatgttt acttgttata gctgatacaa ttggcaaaat tgtattacaa ccaggtgggg
     5761 ttccagcagg tattgtcgta gcaattattg gtgcaccgta tttcttatat ttaatgtaca
     5821 aaacgaaaaa tgtatagtgt caatggacac aacttattgc tatgaaaggc actttattat
     5881 aaggcttttc atagcatttt ttatttaatg agccactcaa gactatttat tttttcaata
     5941 atgaaccatt aagttatcaa gaggatctta tcaaaaatat atttgataac ggtatcaggt
     6001 taattcttta tgatagcgca ttcatttatt ctgttttata ctatgactga taataccaag
     6061 gaggtacaac atgatgaaaa agttaatcaa taaaaaagaa acatttttaa ctgatatgct
     6121 tgaaggattg ttaattgcgc acccagagtt agatctgatt gctaatacag ttattgtaaa
     6181 aaaagctaag aaagaacatg gtgtagcaat agtctctgga ggtggaagcg gacatgaacc
     6241 tgcgcatgcc ggttttgttg cagaaggtat gctagatgca gcggtttgtg gcgaagtatt
     6301 tacatcacct acacctgata aaatattaga agctattaaa gcagtagata ctggtgatgg
     6361 tgtattacta gttgtaaaaa actatgcagg tgacgtgatg aatttcgaaa tggcacaaga
     6421 gcttgcagaa atggaaggta taaatgttca aactgttatt gttcgtgacg acattgctgt
     6481 gacaaacgaa gtacaacgtc gtggtgttgc aggaacagtg tttgttcata agcttgccgg
     6541 ttatcttgct gaaaaaggtt attcattaac agagataaaa tcgcgtgtag aagcgttgtt
     6601 acctgaaatt aaaagtattg gtatggcaat tgagccaccg cttgttccaa ctactggaaa
     6661 atatggcttt gatattgaag acgacaaaat ggaaatcggt attggtatac atggtgaaaa
     6721 aggtattcat agggaagaag taaaggatat tgatcatatt gttggaacat tgttagacga
     6781 attgtataaa gaagttactg ccaatgatgt catattaatg gtaaatggta tgggtggtac
     6841 gccgttatct gaattaaata tcgtaactaa atatattcaa caaaatttag ctgcaagaac
     6901 ggttaatgtt gctaaatggt ttgttggtga ttatatgaca tctttagaca tgcaaggttt
     6961 ttctataact atcgtgccta ataaaccaga atatttggaa gcatttttag caccaacaac
     7021 aagtcaatac tttaaataag aagtgaatat gaataaatat acatttatga ggtggcacaa
     7081 atgaaagtga atgatatgaa agcacgttta ttaaatttag aagaaacgtt taaaaaacat
     7141 gaatctgaat taactgaatt agatcgagca attggtgatg gtgaccacgg ggttaacatg
     7201 gttcgtgggt ttagtagtct taaagacaaa cttgatgata gctcaatgca atcattgttc
     7261 aaatcaactg gtatggcatt gatgtcaaat gttgggggtg catcaggacc actgtatggc
     7321 tttagctttg ttaaaatgtc tgcagtcacc aaagatgata tggataatca agatttcatt
     7381 acactaattc aggcatttgc cgaagcggtt gaatcacgtg gtaaagttac tttaaatgaa
     7441 aagacaatgt atgatgtagt agcgcgagca gcagagaagc ttaaaaatgg tgaaacttta
     7501 acattcaatg atttacagca attagcagat aatacaaaag atatggtagc aacgaaaggt
     7561 agagctgcat attttggaga agaatcaaaa ggttatattg atccaggtgc tcaaagtatg
     7621 gtttatattt taaacgcttt gattggagat gaagataatg cctaaaatta tacttgttag
     7681 ccacagtaaa gaaattgcaa gtggtacaaa atctttgtta aagcaaatgg caggtgacgt
     7741 tgatattata ccaatcgggg gattaccaga tggttcaatt ggaacttcat ttgatatcat
     7801 ccaagaagtt ttgactaaat tagaggatga tgcattgtgt ttttacgata ttggatcttc
     7861 agaaatgaat gtagatatgg caattgaaat gtatgatggt aatcatcgtg tgttaaaagt
     7921 tgatgcacca attgttgaag gcagttttat cgcagcagta aagctatcaa tcggcggttc
     7981 aattgatgat gcattagcag aaatcaaaca atcattttag ttaaaattta ctaataatga
     8041 aaaatgtaaa cctttttcaa atgaaacttt ataaaaaata tgatagtata tatgtaaatg
     8101 tttaataaaa tctggagaaa taggaggaca ttgccatgca acaccttata aaaaaacatg
     8161 tattgaatgg cgagtttgat ttagtacgac aattgatgtc cgaaacagat tttatggaat
     8221 ttgaagaagc atatatttca agtgcgcatg aagtagaaag tatgatgttt tatacatgta
     8281 ttttagatat gattaagtac gaagaatcat ctgaaatgca tgacttagca tttttattgc
     8341 ttgtgtatcc actaagtgaa tatgaaggtg ctttggattc tgcttattat catgcagacg
     8401 cttccataaa acttactgac ggcaaagaag ttaaaagttt gttacaaatg ttattattgc
     8461 atgcgatacc aacacctgtt atttcagata agaaggcttt tgatatcgcc aagcaaattt
     8521 taaaattaga tcctaataat aatgttgctc gtaacgtctt aaaagacact gccaaacgta
     8581 tggacaacgt tgttgttgat ataaatgaat tacaccaacg taatgcacgt taattacatt
     8641 tcaattatat tagcttaata atagttttaa catttggttg ggttgggcat atgttccagc
     8701 cttttttaat acttaaaaac taacgaagta tacttgtgtg cacaaatggt ttttatacaa
     8761 cattttataa atttatacat tttaataaag aacatacgat agatggttta aaccttgtta
     8821 actgagaaat tttgatatgt attcttcgaa atttaactaa atatacgaaa ttcaagaagc
     8881 acaataatta atcatttttc ctatacaaaa gttcgtatga ctgcattata aaagcataaa
     8941 tttataattt ttttaaatgt cattgaacgt gataatgtga atggattgag caattttgaa
     9001 aaagtgaaaa ataacctatg cggcttgcaa ttaattttca gtacgttata atgcacactg
     9061 tgcaaaatta aggaggtcta ttattcacat gatgatgaat aaagaagcaa caaaaattgg
     9121 atttgcctac gtcggcattg tagtgggcgc aggattttca actggacaag aagttatgca
     9181 atttttcact aaatatggct tgtgggctta tttaggtgtt attatatctg gttttatttt
     9241 agcttttatt gggcgccaag tagcaaaaat tggtactgcc tttgaagcga caaatcatga
     9301 atcaacatta caatacgtat tcggtgaaaa gtttagtaaa gtctttgatt atattttaat
     9361 cttcttctta tttggtatag ctgtaaccat gatagctggt gcaggcgcaa catttgaaga
     9421 aagttataac atacctacat ggctaggtgc tttaattatg acattagcga tttatattac
     9481 gttgctatta gactttaata aaatagtacg tgcactaggt atcgttacac catttttaat
     9541 tgttttagtt gtattaatcg ctggcgttta tttatttaaa ggtcatgttt cattagcaga
     9601 agttaaccaa gtagtgcctg aagcaagtat ttggaaggga atctggtttg gtacaatata
     9661 tggtggatta gctttttctg taggttttag taccatcgta gcaatcggtg gggatactga
     9721 aaagcgtaca gtgtcaggtg caggcgcgat gtatggtggt attatctata ctgtattact
     9781 agcattgatc aactttgcat tgcaaagtga atatccaact attaaaaatg cctcaattcc
     9841 tacattgacg ttagcaaata atatccatcc tttaatagca acagtgttat ctgttattat
     9901 gctggcggtt atgtataata ctattctagg actaatgtat tcatttgcag cacgttttac
     9961 agaaccatac agtaaaaatt atcatatctt tattattata atgatggtag caggttattt
    10021 attaagtttc gtaggatttg ctgaattaat taataagtta tatacaatta tgggatatgt
    10081 aggcttattt attgtagtag ctgtaattat taaatatttc aaacgtaaaa atgcggataa
    10141 aaaacatatt gcttaatatc atatgaggga tatccgaaac tttacaattg aatcactttg
    10201 ttttaacctt aaaagcaatt cgtctctact cttatcgggc gaattgcttt ttatatttat
    10261 tcagtctatt aatatgagcg tctaacaaat agagaggtac gatgtaatga ataaagataa
    10321 taaatggacg atgataactg cgctttttat aactgtaatc agtgtattgt tagcatttca
    10381 tctgaaacaa cattatgacc aaattacaaa tgagaaccat gctaataaag acaaaattaa
    10441 tattaaaaat aaaaatgtgc gcatttatca aaaccttaca tacaatagag ttttccctaa
    10501 cagtaaatta gatattatta cacctgttga tatgtcttct aatgccaaac tgccagttat
    10561 tttttggatg cacggtggtg gttatattgc gggtgataag cagtataaaa acccattatt
    10621 agcgaaaatt gctgaacaag ggtacattgt tgtgaatgta aattatgcat tggcgccaca
    10681 atataaatat cccacaccat taattcaaat gaatcaagca actcaattca ttaaagaaaa
    10741 taaaatgaat ttacctattg attttaatca agtaattatt ggcggtgatt ctgcaggtgc
    10801 tcaattagct agccaattta cggcaataca gacgaatgat cgcttaagag aagccatgaa
    10861 atttgatcag tcattcaaac catcgcaaat taaaggtgct atactatttg gtggttttta
    10921 taatatgcaa acagttagag aaactgagtt tccaagaata cagttattta tgaaaagtta
    10981 tactggcgaa gaagattggg aaaagagttt taaaaacatt tcacaaatgt cgacagtaaa
    11041 acaatcgaca aaaaattatc caccaacatt tttatctgtt ggagatagcg atccattcga
    11101 aagtcaaaat atagaattca gtaagaaatt acaagaattg aatgtaccag tagatacttt
    11161 gttttatgat ggtacgcatc atttacatca tcagtatcaa tttcacctta ataaacctga
    11221 atcgatagat aatatcaaaa aagtgttact tttcttaagt cgtaatacat cctctagtgg
    11281 tattcaaact gaagagaaac cacaaataga aaatccgagt aatgaattac cgttaaatcc
    11341 tttaaactaa tgataaacag tagtaattta ttgcttaagc aacatttaag attttcaaat
    11401 taaaaacgaa gaatttaaaa catgtggtgc taatgtgtaa gagtctgaga tataataaat
    11461 tgttagcggt tctttatcat ttctatctca ctctattatg atgtgacatt tattttacaa
    11521 actaatttgt tttgaacctg aaaataactt tttacaacta aattgtcgaa aaacagtgtg
    11581 tacatgatat aataaattta taaattgaaa agaattcaaa agaaaaatta aaatatagat
    11641 tgagcacgaa gtgatttgaa ataaggttgt gaaagggaat gacaaggtca gcattaaaac
    11701 catttaaaaa taaacgcgtt atggttactg gacgtataca acgtgttttg tttaaaaatt
    11761 atttagatag acatagcaca tttaagccga atgtaaggat attattaaaa gatgtatttg
    11821 tttcaggtgt atcaatagat catttatggt tatatgagac aaataaatac tatgcattgg
    11881 caatggaact tattcatcaa cgagtaaaat ttagtgcgaa tgttgtaccg tattacaaaa
    11941 taaatagaaa taataattta ttcgtacaag attatggaat taagcgtaaa ggtaggttaa
    12001 ttactgaaga agcttacaat caaaacaatc agtatcagga taagatatat gaaaaattac
    12061 cggatataga ttttagactc gaagattttt atagtaagga aaactaaaca acatatataa
    12121 agcaccacta atcgtagttg atagttaatc aattacacat tagtggtgtt ttattttgaa
    12181 tttaaatttg ataaatgaat aagagtgtac gtatttattt gagattagat aacattgata
    12241 agtatgagta atgtattagg taaccattaa attcgttgtt atacgattct gatacaagat
    12301 tatgataaaa tagctttaat taagaatttt agccatcata tagtcatcat aatatttacc
    12361 atcgataaat aacttatctt ttaaaacgcc ttcgatttga aaatcggcac ttttaaaaag
    12421 ctcgagggca ggttggttat tgagtggtac atttgcttca attcggtgta tttgattgtt
    12481 taaacaccaa gccataatgg catcaagaag tgcttggcca attccacgat gttgatataa
    12541 tttctttaca cctaaatcaa ttttagcaac atgtttaatg cgttgaaatg gtgtcgtatt
    12601 aacaaaggca aagccaacga gttgttcatc actttcagca acgaagatga ctttatgcgg
    12661 agaagtgata tattcttcta attgtttact agccgatgtg acgctaggat catattctcc
    12721 tggtgtgtag aacatatacg gagattcgtc gtatatgttc gctaacattg aaatgaaatt
    12781 ttctacatct ttgatactaa ctctacgtat aatatgggcc ataaaaagcc tccagtattt
    12841 tgaaatttta aaacatttgc tactattata atatatatgt aactaaaagg tggagtaata
    12901 tgttttttgg ttacagttta aaggagtatt ttagatgaaa cctaaagttt tattagcagg
    12961 tggaacagga tatattggta agtatttaag tgaagtgatt gaaaatgatg ctgaactttt
    13021 tgctatatca aaatatccag acaataaaaa aacagatgat gttgaaatga cttggattca
    13081 gtgtgatata tttcattacg aacaggttgt tgcagcaatg aatcaaatag atattgctgt
    13141 attctttatc gacccaacaa agaattctgc caaaataaca caatcatcag caagagattt
    13201 aacattaatc gcagcagata attttggtcg agcagcggct atcaatcaag taaaaaaagt
    13261 aatctacata cctgggagtc gttatgataa tgaaacaatt gaacgcctag gtgcatatgg
    13321 tacacctgta gaaacaacaa atttagtttt taaacgttct ttagttaatg tagaattaca
    13381 agtttcaaag tatgatgatg ttagatcaac gatgaaggta gttttaccaa agggatggac
    13441 attaaagaac gttgtaaacc attttattgc atggatgggt tacactaaag gaacttttgt
    13501 gaaaacagaa aaatcacatg atcaatttaa gatatatatt aagaataagg tgcgaccgct
    13561 cgcagtattt aaaatagaag aaacagctga tggaataata actttaattt tattgagtgg
    13621 aagtttagtg aaaaaatata cagttaatca agggaagtta gaatttagat taatcaaaga
    13681 gtcggcagtc gtttatatac atctatacga ttatatccct cgattatttt ggccgattta
    13741 ttactttata caagcaccaa tgcaaaaaat gatgattcat ggctttgaag ttgactgccg
    13801 gattaaagat tttcaaagtc gattaaaatc aggagaaaat atgaaatata ctaaatgata
    13861 ttgggtgata tggatgcaaa tactactagt agaagatgac aatactttgt ttcaagaatt
    13921 gaaaaaagaa ttagaacaat gggattttaa tgttgctggt attgaagatt tcggcaaagt
    13981 aatggataca tttgaaagtt ttaatcctga aattgttata ttggatgttc aattacctaa
    14041 atatgatggg ttttattggt gcagaaaaat gagagaagtt tccaacgtac caatattatt
    14101 tttatcatct cgtgataatc caatggatca agtgatgagt atggaacttg gcgcagatga
    14161 ttatatgcaa aaaccgttct ataccaatgt attaattgct aaattacaag cgatttatcg
    14221 tcgtgtctat gagtttacag ctgaagaaaa acgtacattg acttggcaag atgctgtcgt
    14281 tgatctatca aaagatagta tacaaaaagg tgatcagacg attttcctgt ccaaaacaga
    14341 aatgattata ttagaaattc ttattaccaa aaaaaatcaa atcgtttcga gagatacaat
    14401 tatcactgca ttatgggatg atgaagcatt tgttagtgat aatacgttaa cagtaaatgt
    14461 gaatcgttta cgaaaaaaat tatctgaaat tagtatggat agtgcaatcg aaacaaaagt
    14521 aggaaaagga tatatggctc atgaataatt tgaaatgggt agcttatttt ttgaaatctc
    14581 gcatgaactg gatattttgg atattgtttt taaacttcct tatgttaggc attagtctaa
    14641 tcgattatga ttttccaata gacagtttat tttatattgt ttctttgaat ttaagtttaa
    14701 caatgatttt tcttttattg acatatttta aagaagtaaa attatataag cattttgaca
    14761 aagataaaga aatagaagaa attaaacata aagatttagc ggaaacgcca tttcaacgtc
    14821 atacagttga ttatttatat cgtcaaatct cagcgcacaa agaaaaggtt gttgagcaac
    14881 agttgcaatt gaacatgcat gaacaaacca ttacagaatt tgtgcacgac ataaaaacac
    14941 ctgtgacagc tatgaaatta ttaattgatc aagaaaaaaa tcaagaaaga aaacaagcat
    15001 tactatatga atggtctcgt ataaactcga tgctagatac acagctgtat attactagat
    15061 tagaatctca acgtaaagat atgtattttg attacgtgtc acttaaacgc atggtcattg
    15121 atgaaataca attaacaaga catattagtc aggttaaagg tattggtttt gatgttgact
    15181 ttaaagtgga tgattatgtt tatacagata taaaatggtg tcgtatgatt attagacaaa
    15241 ttttgtcaaa cgcattgaaa tatagtgaga attttaatat tgaaattggg acagaattaa
    15301 atgatcaaca tgtttcgtta tatattaaag actatggcag aggtattagt aaaaaagata
    15361 tgccgcgaat atttgaacga ggatttacgt caacggctaa cagaaatgaa acgacgtctt
    15421 caggtatggg tctatattta gtaaatagtg taaaggatca attaggtatt cacctgcaag
    15481 tcacgtcgac tgttggtaag gggacaactg tcagattgat tttcccatta caaaatgaaa
    15541 ttgttgaacg catgtcggaa gtgacaaatt tgtcatttta aacatgcgtt ttgttactta
    15601 gaattgatac atcaatgcag cttcaacgtt ataataagat agatgttagt catatgttaa
    15661 attgaagata caagtgccaa agcctaaagg aaatgaagtt aagataaatt ataggagtgt
    15721 taaagtggca attttagaag taaaacaatt aacaaaaata tatggaacta aaaaaatggc
    15781 acaagaagtg ttgcgagata tcaatatgtc tattgaagaa ggcgagttta ttgctattat
    15841 gggtccctct ggatctggga aaacgacatt attaaatgtt ttaagttcaa ttgattatat
    15901 ttcacaaggt tctattacat taaaaggaaa aaaattagaa aagctttcaa acaaggaatt
    15961 atctgatata cgcaagcatg atattggttt tatttttcaa gagtataatt tactgcatac
    16021 attgactgtt aaagaaaaca taatgttacc actaacggta cagaagttag ataaagaaca
    16081 tatgttaaat cgttatgaaa aagtagcaga agcattaaat atattggata ttagtgataa
    16141 atatccctct gaattgtctg gtggacaaag gcaacgaaca tcagctgcca gagcatttat
    16201 aacattgcct tctattatat ttgctgacga accaacaggt gcattggatt ctaaaagtac
    16261 tcaagattta ttaaaacgat taacaagaat gaatgaagca tttaagtcta caattattat
    16321 ggtaacgcat gatcctgttg cagcaagcta tgcaaatcga gtagtgatgc taaaagatgg
    16381 tcaaattttc actgaattat accaagggga tgacgataaa catacctttt tcaaagaaat
    16441 aatacgtgta caaagtgttt taggtggcgt taattatgac ctttaacgag ataatattta
    16501 aaaatttccg tcaaaattta tcacattatg ccatctatct tttttcgtta attacgagtg
    16561 tagtattgta ttttagcttt gtagcattaa aatacgctca taaactaaac atgacagagt
    16621 catatccaat tataaaggaa ggctcacaag tcggaagcta ctttctattt ttcatcataa
    16681 ttgcattttt gttatatgcc aatgtgttat ttattaaacg acgaagttat gagcttgcat
    16741 tatatcaaac attaggttta tctaaattca acattattta tatactaatg ctcgaacaat
    16801 tactaatatt tataattacg gcaatattag gtattattat tggtattttt ggttcgaaac
    16861 tgttattaat gattgtcttt acattattag gaattaaaga aaaggttcca attattttta
    16921 gtttgagggc ggtatttgaa acattaatgt taatcggtgt cgcttatttt ttaacatctg
    16981 ctcaaaattt tatattagtg ttcaaacaat ctatttcaca gatgtcaaag aataaccagg
    17041 ttaaagaaac aaatcataat aaaattacat ttgaagaggt tgttttaggc atcttaggta
    17101 tagtattgat taccacagga tactatctat ctttgaacat tgttcaatat tatgattcta
    17161 tcggtacact tatgtttatt ttattgtcaa ctgtgattgg ggcatactta ttttttaaaa
    17221 gctctgtttc tctagttttt aaaatggtga agaagtttag aaaaggtgtt ataagtgtaa
    17281 atgatgtcat gttctcatca tctattatgt atcgtattaa gaaaaatgct ttttcactta
    17341 cggtcatggc aatcatttca gcgattactg tttcagttct ttgctttgct gctataagta
    17401 gagcgtcctt atcaagtgaa ataaaatata ctgcaccaca cgacgttaca attaaagacc
    17461 aacaaaaagc taatcaatta gcaagtgaat taaacaatca aaaaattcct catttttata
    17521 attataaaga agtaattcat acgaaattgt ataaagataa tttatttgat gtaaaagcga
    17581 aagaaccata caatgtaaca attactagtg ataaatacat ccctaatact gatttgaaac
    17641 gtgggcaagc tgatttattt gtagcggaag gttctatcaa agatttagtg aaacataaga
    17701 agcatggtaa ggcaattata ggaacgaaaa aacatcatgt taatattaag ttacgtaaag
    17761 atattaataa aatctatttt atgacagatg ttgatttagg tggaccaacg tttgtcttaa
    17821 atgacaaaga ctatcaagaa ataagaaagt atacaaaggc aaagcatatc gtctctcaat
    17881 ttggattcga tttgaaacat aaaaaagatg ctttagcatt agaaaaagcg aaaaataaag
    17941 ttgataaatc tattgaaaca agaagtgaag cgataagctc aatatcaagt ttaaccggaa
    18001 tattattatt tgtaacatca tttttaggta ttacattctt gattgctgta tgttgcatta
    18061 tatacataaa gcaaatagat gaaaccgaag atgagttaga gaattatagt attttgagaa
    18121 agcttggatt tacacaaaaa gatatggcaa ggggactaaa gtttaaaatt atgtttaatt
    18181 ttgggttacc tttagttatt gcactatcac atgcatattt tacatcatta gcatatatga
    18241 aattaatggg tacaacgaat caaataccgg ttttcatagt aatgggatta tacatttgta
    18301 tgtatgctgt ttttgcagtg acggcttata atcattccaa gcgaacaatt agacattcca
    18361 tataaaatat acagatggct ttcagtagag tagtggattc ggattcacga actatactgg
    18421 aagcttttta ttataaatga agagaagtta tatttttagc atgtatagtt gaatactggg
    18481 ttaaaatacc atattaataa tgaagtaaag gtatgagtga ttatgaaagt gttttgaatg
    18541 aaatatattt aattggtgat gcttttaatt gaaaagatta acaggattca actttgtaaa
    18601 ttgtattaaa tgtgagaaaa taaaagtata ttcattgaga gatatatgag tcaatgatcg
    18661 ttttaaacaa gataagtgta ttttaatatg taaaagttat gtaataaata ttgtatcgtt
    18721 gcaaatttcc caactatatc catttacaat ttttagagtt gtatagataa aaaattgcct
    18781 atacaataac tttttcaaaa caaatgccta ctactattta aattttatgt aaaagttaag
    18841 tttcggaatt gttaaaaatt tgtaaattga attgtgggat tgtaaatttt gtgctatttt
    18901 catataaggg caaatacagt aaattgttta ctgtgaggca tttttgaaat taatatcagt
    18961 acactaaaat tatactgacg attgataata acaaatttgt atcattagtt tgtaaattca
    19021 cttgtcttaa tttgaaacaa attataactg aatgtgattg gtgacaatcg cttaaatgga
    19081 ggattttaaa tgtttagtaa gaaaaaagat aagtttatgg ttcaattaga agagatggtt
    19141 ttcaatctgg atcgtgctgc tattgaattc ggtaaaatgg atttcaatac acatttagat
    19201 ttaaaagcat actcagacaa cattaaaact tatgagtcac atggtgacga attagtacat
    19261 caagtaatta ctgatttaaa tcaaacattt atcacaccaa ttgaacgtga agatatttta
    19321 tcattatgtg atgcaattga tgatgtttta gatgcaattg aagaaacggc agctatgttt
    19381 gaaatgtatt caatcgaata cacagatgaa tatatggctg agtttgttga taacattcaa
    19441 aaagcagttg cagaaatgaa acttgctgtc ggcttattag tcgataaaaa attatcacat
    19501 atgcgtattc attcaattaa tattaaagaa tttgaaacaa actgtgatgg tattttaaga
    19561 cagtcaatta aacatatttt caatagcgaa acagatccaa tcactttaat taaaataaaa
    19621 gatatttatg aaagcatgga agaaatcgct gataaatgtc aaatcgtagc aaataatttt
    19681 gaaactatta ttatgaaaaa t
//
LOCUS       DAKTLH010000036        19168 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.036, whole genome shotgun sequence.
ACCESSION   DAKTLH010000036 DAKTLH010000000
VERSION     DAKTLH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19168
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.036"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            314..688
                     /locus_tag="O0394_001093"
     CDS             314..688
                     /locus_tag="O0394_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCY0499182.1"
                     /translation="MKRILVVFLMLAIILAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDENVEVEKD"
     gene            841..1239
                     /locus_tag="O0394_001094"
     CDS             841..1239
                     /locus_tag="O0394_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCY0499183.1"
                     /translation="MEKSIKIMTIIGIVVQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLSLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLISGIKLLTNNKPKDEISDLS"
     gene            complement(1455..2279)
                     /locus_tag="O0394_001095"
     CDS             complement(1455..2279)
                     /locus_tag="O0394_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCY0499184.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(2516..3823)
                     /gene="brnQ"
                     /locus_tag="O0394_001096"
     CDS             complement(2516..3823)
                     /gene="brnQ"
                     /locus_tag="O0394_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCY0499185.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRLFGSFGNLV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKSFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT"
     gene            4407..5297
                     /locus_tag="O0394_001097"
     CDS             4407..5297
                     /locus_tag="O0394_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HCY0499186.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            5546..6595
                     /locus_tag="O0394_001098"
     CDS             5546..6595
                     /locus_tag="O0394_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCY0499187.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASSVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTVILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            6608..7285
                     /locus_tag="O0394_001099"
     CDS             6608..7285
                     /locus_tag="O0394_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0499188.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKQRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            7493..8524
                     /locus_tag="O0394_001100"
     CDS             7493..8524
                     /locus_tag="O0394_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCY0499189.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPTTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            8867..9985
                     /locus_tag="O0394_001101"
     CDS             8867..9985
                     /locus_tag="O0394_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCY0499190.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKNLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            9960..10883
                     /locus_tag="O0394_001102"
     CDS             9960..10883
                     /locus_tag="O0394_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCY0499191.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVIAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            10894..12114
                     /locus_tag="O0394_001103"
     CDS             10894..12114
                     /locus_tag="O0394_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCY0499192.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKKIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINISKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     TDVEIDNLTKSTETKTLNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(12219..13751)
                     /locus_tag="O0394_001104"
     CDS             complement(12219..13751)
                     /locus_tag="O0394_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HCY0499193.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVVNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(13791..14672)
                     /locus_tag="O0394_001105"
     CDS             complement(13791..14672)
                     /locus_tag="O0394_001105"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCY0499194.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            14830..15690
                     /locus_tag="O0394_001106"
     CDS             14830..15690
                     /locus_tag="O0394_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCY0499195.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIAGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(15968..16768)
                     /locus_tag="O0394_001107"
     CDS             complement(15968..16768)
                     /locus_tag="O0394_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0499196.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            16907..17575
                     /locus_tag="O0394_001108"
     CDS             16907..17575
                     /locus_tag="O0394_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCY0499197.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     IMED"
     gene            complement(17702..19015)
                     /locus_tag="O0394_001109"
     CDS             complement(17702..19015)
                     /locus_tag="O0394_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCY0499198.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPISNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQALLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
BASE COUNT         6782 a         2919 c         3543 g         5924 t
ORIGIN      
        1 aaatatttgg gaattaaata aatatatagg aggcaatttt tagagataca tagcaatgta
       61 aaggtatcaa atagttattt cataatttat tatgatgaca taattttcag tagatagttg
      121 taaattaaca ctgaaataga tgtttatatt aagaagactt taatatcata attaatcaaa
      181 atcttgcgtc aaacatttaa acgaatttta agttgataac aattggcaaa acattgttca
      241 aacatcacaa tgataaagca tattatcagt attgtagtgt gtggaaaatg acagccatct
      301 aaggagaaaa atgatgaaaa gaatattggt agtattttta atgttagcaa ttatattggc
      361 aggttgttct aataaaggtg aaaagtatca aaaagatatt gataaagtgt acaaagaaca
      421 gaatcaaatg aataaaattg cctcgaaagt acaaaacact attaaaacag acattaaaca
      481 agaagacagt aatacacatg tttataaaga tggtaaagtc attgttattg gtattcaatt
      541 atataaagat cgtgaaaaaa tgtattattt cgcatatgaa ataaaagatg gtaaggcaga
      601 gattaataga gaaatagacc caattaagta tatgaaagac cataaagcag attatgaaga
      661 tgaaaatgta gaagtggaaa aagattaaca taggctttct ttaaaacttt taaccagttt
      721 caaaaagtaa atgagcttta gcgacaataa tttttaaaat aaacacgata taaatttaaa
      781 catttatata taagaacaca aacgcaagtt tgtaaataat aataataaag gagtaagaca
      841 atggaaaaat cgatcaaaat aatgacaata ataggaattg ttgttcaggg tttagcaacg
      901 gtatttagtt tactattgat ggttttagca gcatcaggtg taatgactac agatgtgtca
      961 acaacagtta atggtgaggt tgacccagtt gatgcagaaa cagcagcagc aattttcact
     1021 gtattattct tatccctatt catatttgga atcatttcaa ttattttagg tgcaatcggt
     1081 atgtttaaag catctaaaaa caaaaaaatg agtggtatat tgttgattat tggagctgta
     1141 ataagtggta acataattac atttgcttta tggttaatca gtggtattaa actacttact
     1201 aataacaagc ctaaagatga aataagcgac ttatcataaa catcgtatat tgaaatttca
     1261 agatttctta agtaattaaa taaagtgctc tcttagaagt agattttcag tcaataactg
     1321 cttttaagag agcattttgt atttgataat gctcatagca tcggataaaa atcattgacg
     1381 aactttaaaa aaatacgtta gacaatgcac ttctaaatat taaaggcatc gactaacgca
     1441 ttgttcaaat atagttaatt atttttataa aattgatgat gatcattcaa gtaagcatag
     1501 aataaaccta taatgagtcc gcctccaata tagttaccga taaaagccgc agcgatattc
     1561 gaaatagctg gtatgaagtg caatgtatca acttgataaa ttaaaccacc cataaataag
     1621 caactgttgt aaacgacgtg ttcataaccc ataaaggcga atatggtaac accgaacatc
     1681 atgacaaaca tttttgcgag tacatcgtca atttgcatgg caataactaa tgaaatattg
     1741 ataaagaaat tggcgaagat cgctttcatt aatatactta caaaaccagt agacaacgtt
     1801 ttatgctcta taactgctga taactgattt aacatatctg gcgtcattac atttgaaaaa
     1861 cgcatgaaac taaataaaat agcagcacct aaaatatttc ctgcaaagca taataaaaat
     1921 attttcaata ctctagttgg tttaattact ttataataca ggcctacagt aaagtacatg
     1981 aagttactgg ttagtagttc ggagtttgta aataaaatga gtactaacgc aaagctgaat
     2041 gtaatggcac tggccatatt cacaatgcct ggcggtaaat ctggttcgtg tgttgcttta
     2101 actgataata cgaagaccgt aataatcccg ataataaatc ctgccatcat cgcgcgtaat
     2161 aaataacgtt ttaaataaac gctttgtaat atatcttttg ttcttatcgt ttcgactacg
     2221 ttatttaccc agtcgtcccc ataaaaaatc ttatcccatt taatatgttt ctccttcaca
     2281 atgacatcct cacttttcat ttgttaatca gagtatacta tgaattgcct tatttgtgaa
     2341 taatttcaca aaattaattt taaaaactaa tatttttgaa taacagctag gattttttaa
     2401 caaatcattt attttaaaat aatcaataga cagacggtat cactgaaaag tcatgaattt
     2461 catataaaaa agcttagtaa cgatgggtca atcattacta ggcagtgagc atttattaag
     2521 ttgtcgcttg tttcggacgg cgtataaata catcgattat gaaaccgata atagcaaaga
     2581 gcatgaatgg tacaagccaa gctaaatcga tatctgctaa aggtaacatc ataaacgatt
     2641 tcaaaataac accgtgtaat aagttgaaac tatttagtat ttgtaaaatt gaaataatca
     2701 atgtaataac agttgcgagt cgataggccc aactgaatct gaatgtgcta aacatgttag
     2761 caaatgatat gagtacaagt gcaatcgaca cgggatatat taaagtcaat aatgggacag
     2821 caatttttaa aatcatttct aaaccaagtg ttgtaaataa gaaccctatg atagagaaaa
     2881 taagtgcgaa tattttataa gaaaacttag gtacgtgttt cttagtaaat gtggcgcaag
     2941 cattgacgag tcctatacat gttgttaggc atgcaaggat aaccgtcatt ccaaatacga
     3001 ggttaccgaa cgaaccaaat aatcgtaatg agttgtacgt caatatatct gtaccatctt
     3061 taaagtttcc tggagctgtt gatgccccaa cgtatgcaag tgcaaagtaa atcattccaa
     3121 gtaatatggc tgcaataaga cctgaaaagc agacatattt taaaattttc atgcgatctg
     3181 tgaggccttt aaacttatag ccattgacaa tgactacgga aaaagctaac gcagcaacaa
     3241 gatccattgt aaaatagcct tccaaacttc ctgaaatgaa aggatgtgtt atatatttat
     3301 ccttaggtgc acttagtgca gattcagggt tgaaaatgac agcaatactt aatagagcga
     3361 ccattaatag taataacggt gttaataatt tacctaaatt atcaacgatt ttcgatggat
     3421 ttaaactaat ccagtaaacg atggcaaaaa agattgctgc gaatataatt aaagtccatt
     3481 ggttatgcac aggtaaaatg tgtcttgtac caatttcgta cgcgacattt gcagcacgtg
     3541 gaataccgta aaatgctccg atagacatgt aaatcacgac agcaaaaata aacccgaacc
     3601 atggatgtat acgattgcct acactttcaa caccttcatc ataaaatgca acaacaataa
     3661 cagtaataaa ggggagtaat atgcctgtaa gggcaaagcc tagcatacca atccacatat
     3721 tttgacccgc tgtatggcca agcatgggcg ggaatattaa atttccggct ccaaaaaata
     3781 gtgaaaataa catgaggccc gaaataataa cttgtttttt caatgtaatt tccttcctaa
     3841 aactagatat tcataataat ttaaaaaatc tgaaaaataa aaacgttctt actttatctt
     3901 taaatgacaa tactatgatt ctattatttt ataaaaaaga ttgcaataat aacgtcttgc
     3961 tattattgga taatgacaat taaagtgtcg tttttatgaa ttaaaatagg tgaaagtaag
     4021 ttggaaatac gttggagcgt tgtgattttt atgagaaatt gtatgagtga ggactgctat
     4081 ttataaaagg taaactatgg atgagtttat ggttgttcca ataggtaaat aagagatagc
     4141 acactgtaca cataatgata cgtggatgag tgaatatgtc tgataggaag gcctagtcac
     4201 tcaaatgaat ttaagtttat tacgtgataa atcacaaatc tctctcatgt gataggtctc
     4261 ccattaaatc atgcttatat gaaatgttca catatttgtt agcttttcaa gaaataatat
     4321 taaatatctt ttcatagtat agcaatatta aataaatgca tttataattt taactgtatt
     4381 ttaaatatta atcatgaggt gataagatga ataaaatttc aaagtatatt gcaatagcat
     4441 cattatcggt agcggttaca gtttcagcac cacaaacgac aaattctaca gcgtttgcca
     4501 aaagttctgc tgaagttcaa caaacgcaac aagcttctat accagcatca caaaaggcga
     4561 atcttggtaa tcaaaatatt atggcagtgg cttggtatca aaattcagct gaagcaaaag
     4621 cattatattt acaaggttat aacagtgcaa agacacagtt agataaagag attaaaaaga
     4681 ataaaggtaa acataagtta gctattgctt tggatttaga tgaaacagtt ttagataatt
     4741 ctccatatca aggctatgca tcaatacata ataaaccttt cccagaaggt tggcatgaat
     4801 gggtacaagc tgctaaagct aaacctgtct atggcgcaaa agaattcttg aaatatgctg
     4861 acaaaaaagg tgtcgatatc tactatattt ctgatagaga taaagaaaaa gatttaaagg
     4921 caacacaaaa gaacttaaaa caacaaggta tccctcaagc taagaagagt catattttac
     4981 taaaaggtaa agatgataag agtaaagaat cacgcagaca aatggttcaa aaggatcata
     5041 aacttgtcat gctatttgga gataatttat tagactttac agatccaaaa gaagctacag
     5101 ctgaatctcg tgaagcatta attgaaaaac ataaagacga tttcggtaag aaatatatca
     5161 ttttccctaa cccaatgtat ggtagttggg aagctacgat ttacaacaat aactataaag
     5221 caagtgacaa agcaaaagat aaattacgta aaaatgctat taagcaattc gatcctaaaa
     5281 caggcgaagt taaataatat atgaattgga cgtctacatg tacttaaagg tatatgtagg
     5341 cgtttttaaa ttgatcctta attttaaatg taccgtacaa aaataataga caattaattg
     5401 cattttccat aaatttgctt ttaatataaa aagttcgggt ataatgaagg ataagtgtat
     5461 gtataacatt acagagcgca acacgacgta cgtgcatcta agaatataaa caatgtttaa
     5521 atcatagtag tagggagaga aatgcatgtt tttagcttgg aatgaaatac ggcgcaacaa
     5581 attgaagttt ggactaatta ttggtgtgtt aacgatgatt agttacttgc tatttttatt
     5641 atctggattg gcgaatggtc ttatcaatat gaataaagaa ggcattgata agtggcaagc
     5701 agatgccatt gttctaaata aagatgccaa tcaaactgtg caacaatctg tttttaacaa
     5761 gaaagatatt gaaaataaat acaagaagca agctactttg aagcaaacag gggaaattgt
     5821 gtctaatggc catcaaaaag acaatgtttt agtgttcggt gttgaaaagt catcattttt
     5881 agttccgagt ttaatagaag ggcataaagc gactaaagat aatgaagtgt tagctgatga
     5941 aacgcttaaa aataaaggat ttaaaattgg tgacacatta tcactatctc aatcagatga
     6001 aaaattgcat atcgtaggtt ttacagaaag tgcaaaatat aatgcgtcat cagtcatttt
     6061 cacgaatgac gctaccattg ccaagatcaa tcctagattg actggagata aaattaatgc
     6121 agttgttgta cgtgatacaa attggaaaga caaaaaatta aaccaagagc ttgaagcggt
     6181 aagtattaat gactttattg aaaatttacc aggttataaa ccacagaact taacattaaa
     6241 ctttatgatt tcattcttat ttgtcatttc agctacagtt ataggcattt tcctatatgt
     6301 catgacatta caaaagacga gtttatttgg catattaaaa gctcaaggat ttacgaatgg
     6361 ctatttggcg aatgtggtaa tttcgcagac ggtcatatta gcactatttg gtacggcatt
     6421 tggcttactg ttaacaggcg ttacaggtgc atttttacct gatgcagtac ctgtcaaatt
     6481 cgatgtacta acattgctcg tatttgcaat tgtgttaatg attgtctctg tattaggaag
     6541 tttattctcc attttaacaa ttagaaaaat agatccgtta aaggcgattg ggtaggaggt
     6601 gtagcaaatg ttgaaatttg aaaatgtaac aaagtcattt aaagatggga atcgtaacat
     6661 tgaagcggtt aaagatacaa attttgagat aaataaaggt gatattatag cattggttgg
     6721 accttctggc tctggtaaaa gtacatttct aactatggca ggtgctttac aaacaccgac
     6781 atctgggcac attttaatca ataaccaaga tattacgaca atgaagcaaa aagcattggc
     6841 aaaagttaga atgtctgaaa taggttttat tttacaagct acaaaccttg taccattttt
     6901 aacggtaaag caacaattta cattattgaa aaagaaaaat aagaatgtta tgtctaatga
     6961 agactatcag caacttatgt cacaattagg tctaacttca ttgcttaata agttaccttc
     7021 agaaatttca ggtggtcaga aacaacgtgt ggcgatagcc aaagcgttat atacgaatcc
     7081 gtcgattatt ttagcggatg aacctaccgc ggcgttagat actgaaaatg cgattgaagt
     7141 cattaaaatt ctacgtgatc aagccaaaca aagaaagaaa gcatgtatta ttgttacaca
     7201 tgatgaacga cttaaagcat attgtgatcg ttcatatcat atgaaagatg gcgtccttaa
     7261 tcttgaaaat gaaacagtag aatagtttta ttaagccggt acatcatgtg ccggtatttt
     7321 tatgtttatg tattatttga ataaactttc acattcaatt aataataatt attatcgaaa
     7381 atcagaaata ttccgtgaaa tataatattt tttgtagtaa aatggcctct aagtattcaa
     7441 tatttaaata tggggattga atataaaatt atcgtaatgg gggtcaatgg ttatggattt
     7501 attgataggt actttatttt tatttttggt cttagtgatt tttacattat ttacatataa
     7561 agcgcctaat ggtatgcgtg ccatgggagc attagctaat gcagcaatcg caacattttt
     7621 agtggaagca tttaataaat atgttggtgg cgaagtattc ggtattaaat ttttagaaga
     7681 gctaggagac gctgcgggag gtctaggtgg tgtcgctgcc gctggattaa cagcattagc
     7741 tatcggtgtg tcaccagtat atgcattagt tatagcagcc gcgtgcggtg gtatggattt
     7801 attaccaggt ttctttgcgg gttatatgat tggatatgtg atgaaatata cagagaaata
     7861 tgtgccggat ggtgtcgact taattggatc gattgtcatc ttagcgccat tagctcgtct
     7921 tattgcagta ttattaacgc cagtagtgaa tagtacattg attcgaattg gtgatattat
     7981 ccaaagtagt acgaatacga atccaattat catgggtatc attttaggtg gtattattac
     8041 ggttgtcggc acagcgccat tgagttcaat ggcattgaca gcattattag gtttaacggg
     8101 tgtacctatg gctattggtg ccatggcagc atttagttcg gcatttatga atgggacgct
     8161 attccatcgc ttaaaattag gtgatcgtaa gtctacgatt gcagtaagta ttgaaccttt
     8221 atcacaagca gatattgtat cagccaatcc aattccaatc tatattacaa atttctttgg
     8281 tggtgcgatt gctggtttaa ttattgctat gtcaggttta attaacgatg cgacaggtac
     8341 agctacaccg attgcaggat ttttagttat gtttggattt aatcatccga cgacaattgt
     8401 gatttatggt gtagtaatgg cgattgtagg tgcgcttgca ggttatcttg gttcaattgt
     8461 atttaaaaaa tatccaattg ttactaagca agacatgatt aatcgaggtg cagtagacgc
     8521 atagcatcat catattgaat agtaaaaaca aataaaacat agtaacgtga ttcagtcgat
     8581 gtaacagtcg ataatgagtc acgttttttt atagaaaaat acaagacata aaaatgtcat
     8641 aatttattgt cgacaaatat catactgtat aaacatttat cattttctca agtacctttt
     8701 acacgatgga atgaacttac tttttacgaa attatgcgta ttttataaac aaatatcatt
     8761 gatataacgg taaatgtaag cgtttacaac agaaataaca gcatgctacg atatttttgt
     8821 aaattcactg attcaagtat tttaagtcaa tatgaggagg gatgttatga gcgattctga
     8881 gaaagaaatt ttaaaaagaa ttaaagataa tccgtttatt tcacaacgtg aacttgctga
     8941 ggcaattgga ttatctagac ccagcgtagc aaacattatt tcaggattaa tacaaaagga
     9001 atatgttatg ggaaaggcat atgttttaaa tgaagattat cctattgttt gtattggcgc
     9061 agcgaatgta gatcgtaagt tttatgtgca taaaaattta gttgcagaaa catcaaatcc
     9121 tgtaacgtca acacgctcta ttggtggcgt agcaagaaat attgctgaga acttaggtag
     9181 gcttggcgaa acggtcgctt ttttatctgc tagtggacaa gatagtgaat gggaaatgat
     9241 taaacgattg tccacaccat ttatgaattt ggatcatgtt caacaatttg aaaatgcgag
     9301 tacaggttca tatacagctt taattagtaa agaaggcgac atgacatatg gcttagcaga
     9361 tatggaagtg tttgactaca ttacgcctga atttttaatt aagcgttcac acttattgaa
     9421 aaaggctaag tgcattattg tagatttgaa tttaggcaaa gaggcattaa acttcttatg
     9481 tgcctatacc acgaaacatc aaatcaaatt agttatcacc acggtttctt ccccaaaaat
     9541 gaaaaatatg cctgattcat tacatgctat tgattggatt atcacgaata aagatgaaac
     9601 agaaacatac ttaaatttaa aaatagaatc tactgatgat ttaaaaatag ctgctaaacg
     9661 ctggaatgat ttaggtgtta aaaatgttat tgtgacaaat ggcgtgaaag aactcattta
     9721 tcgaagtggt gaggaagaaa tcattaagtc agttatgcca tcaaatagtg tgaaagatgt
     9781 tacaggtgca ggcgattcat tctgtgctgc agtagtgtat agctggttaa atgggatgtc
     9841 tactgaagat atattaattg ctggtatggt taacgcaaag aaaacgatag aaacgaaata
     9901 tacagttagg caaaacctag atcaacagca actttatcac gatatggagg attataaaaa
     9961 tggcaaattt acaaaagtat attgagtatt ctcgagaagt tcagcaagca cgggagaaca
    10021 atcaaccgat tgtagcatta gaatcaacaa ttatttcgca tggtatgccg tacccacaaa
    10081 atgttgaaat ggcaacaaca gtagagcaaa ttatcaggaa taatggtgcc attccagcaa
    10141 ccatagccat tatagatggc aaaattaaaa ttggtttaga aagcgaagat ttagaaatac
    10201 tggcaactag taaagacgtt gctaaagtat ctagaaggga tttagcagaa gttattgcga
    10261 tgaagtgtgt tggtgctact actgtagcga cgacgatgat atgtgctgca atggctggta
    10321 ttcaattttt tgttacagga ggtattgggg gcgtccataa aggtgcagaa catacgatgg
    10381 acatttcagc agacttagaa gaactgtcta aaacaaatgt cactgttatc tgtgcaggtg
    10441 ccaaatcaat tttagactta cctaagacga tggagtattt agaaacaaaa ggcgttccag
    10501 ttattggata tcaaacgaat gaattgccag cattcttcac tcgcgaaagc ggtgttaagt
    10561 taacaagttc ggttgaaacg ccagaacgac ttgctgacat tcatttaaca aaacagcagt
    10621 taaatcttga aggtggcatt gttgttgcta atccaattcc atatgagcat gccttatcaa
    10681 aagcatatat tgaggcaatc ataaatgaag ctgttgttga agcggaaaat caaggtatta
    10741 aaggtaagga cgccacaccg ttcttgttag ggaaaattgt agaaaaaacg aatggtaaaa
    10801 gtttagcagc aaatataaaa cttgttgaaa acaatgcggc gttgggtgct aaaattgctg
    10861 tcgctgttaa taaattattg taggtgatga tacatgaata ttttattcgc tatcacaggg
    10921 atagcatttg cactatttgt tgcgttttta ttcagttttg atcgtaaaaa aatagacttc
    10981 aaaaagacgt taataatgat atttattcaa gtgttgatcg tgttatttat gatgaacaca
    11041 acgattggtt tgacaatttt aactgcacta ggttcatttt ttgaagggct aataaatatt
    11101 agtaaagcag gcataaattt tgtttttgga gatatacaaa ataaaaatgg ctttacgttc
    11161 tttttaaacg tattactgcc attagttttt atttctgtat taataggcat ctttaattat
    11221 attaaggtat taccatttat tatcaaatat gtaggtatcg ctattaataa aataactaga
    11281 atggggcgct tagaaagtta ttttgctatt tcaacagcaa tgtttgggca accagaagta
    11341 tatttaacaa taaaagatat tattccaaga ttatctagag cgaaattata tacaattgcg
    11401 acgtctggta tgagtgctgt tagtatggca atgctaggtt catatatgca gatgattgaa
    11461 cccaagttcg tagttacagc agtaatgtta aatattttta gtgcgcttat catcgccagt
    11521 gtaatcaatc cctataaatc tgatgatact gatgttgaaa ttgataactt aacgaaatcc
    11581 acagaaacta aaacattgaa tggaaaaaca ggaaaaccta agaaagttgc ctttttccaa
    11641 atgattggtg atagtgcgat ggatgggttt aaaatcgctg ttgtagtagc cgtaatgttg
    11701 ttagcattta tttcattaat ggaagcaatt aatatcatgt ttggtagtgt tggtttgaac
    11761 tttaaacagc ttattggcta tgtgtttgca ccaatcgcat tcttaatggg gattccatgg
    11821 agcgaagctg ttccagctgg ctctttaatg gcgactaaat taattacaaa tgagtttgta
    11881 gcaatgcttg attttaaaaa tgtcctgggt gatgtatcag ctcgaacaca aggtatcatt
    11941 tcagtttact tagtaagctt cgctaatttt ggtacggttg gtatcatcgt aggttcaatt
    12001 aaaggcatta gtgataaaca aggagaaaaa gttgcatcct ttgcaatgag gttgctactt
    12061 ggttcaactc tagcttcaat catttcagga tcaatcattg gcttagtatt gtaaatgaat
    12121 cgaagtacct aaattaaatt catggcaaag ctaaaccccg tcaccaagtt ggcgcaacag
    12181 cgcatcataa cttagtgacg gggttttatc ataacaatct actttttcgt agccgttttt
    12241 gaaatgtatg ttgatggttt atctttttca aaaattgtta atcccgttat atctttttta
    12301 tgttttgaag ggacaatgaa gctaagtata taagcaaaga caaaagcaac tgtaaatgaa
    12361 atggtagata catagaaagg tgagttacct ttgccaacac cattatagac ataagcaaag
    12421 atgataccca atattaatcc acaaataaca ccgaatgtat tcgtacgttt agtgaaaata
    12481 ccaactgcaa atacaccagc caatggaacg ccgaataatc cagtcacaaa caagaataaa
    12541 tcccataagt catttgaatt agaagcaatt aagtatagtg acattccaaa accgaaaata
    12601 cctgcaatga taataatgaa acgtgcaaag ttaacttcgt gtcgctcgct accttttccg
    12661 aagaagcgtt gcttaatgtc gattgaaata caagcagata tagaatttaa actagatgaa
    12721 atggtagact gtgcagcggc gaaaatggct gcaataagta atcctgctac aaatggtggc
    12781 atctcagtca aaatgaaata tggcactaca gatgatgtat tgaagccttt tggtaaaaca
    12841 gcttcatgtg tataaaatga atacagcatt gtacccatac cataaaataa gggtgctgaa
    12901 attaaagcta ggataccatt tgtccataac gatttatttg tttcttttaa actatcagaa
    12961 gcttgataac gctgcacgac gtcttgactc gctgtgtatt gatacaagtt gttgaaaata
    13021 tttcctagga aaataattgg aatggcagct gccgcagtat ttagtttcca attgtctgca
    13081 ctaattaatt ttttgtgctc aatcgcatct gcaaagacag tgccgaaacc gcctttaatg
    13141 ttcacaacac ctagaataat aataactaaa gcgccgccta ataaaatgac gccttgaatg
    13201 aaatcactcc aaaccacacc ttcgaaacca cctaaaaatg tatataaaat acatagtaaa
    13261 ccaacgagtg atgcaacgat ataagggttc atgtctgata cagatgtgat tgctaatgtt
    13321 ggtaagtaga taacaattgc aacacgccct aaatggtaaa cgacaaataa taatgagcca
    13381 atgacacgta tgctagggcc aaatctagct tctaaatatt catatgcaga tgttaccttt
    13441 aactttttaa agaaagggac atagaaataa ataagtaatg gaataattgc gacgatagca
    13501 atgttaccag cgatatatga ccaatctgtt aaaaatgctt tctctggtgt cgacataaat
    13561 gtaatcgcac ttaacgtagt agcataaatt gaaaagccaa ctacccaaga tggcaagcga
    13621 ccacttgcgg taaagaaact attggtactt tggctcgcgc gcttggtaaa ataaacgcca
    13681 atgaacaaca tagctagtag ataaatgata acggcaaccc agtttagtgt gccaaatcca
    13741 acttctttca tgggcaacat cccctttaca atgtattgat tctttgatgt ctataaatcg
    13801 tattttgcaa tgagttgatc taatgtttgt cgatgtgctt cgttaaaagg tttgaaaggt
    13861 cttttcggta atcctgcatc aatgccacga tgacgtaata tttctttcaa tgttggataa
    13921 atccccattg ataacactgt ttcgataatg tcgtttgaat catgttgcag ttggtaagct
    13981 tcttgaattt gaccttgtcg tgctaagtcg aagatttttc ttgcacggcg accattaacg
    14041 ttatatgtag aaccaattgc accatctacg ccagaaatcg tagcttgaac taacatttca
    14101 tcaaagccag ataagattaa tttgtctggg aatgcttttc taatacgttc gagtaggaag
    14161 aagtttggcg ctgtatattt aacaccaaca attttttcat gattaaatag ctcgctgaat
    14221 tgttcaatag aaatattcac acctgttaaa tctggtattg cataaataat catattgttc
    14281 tgagttgctt cgataatatc gaaatagtaa tctctaattt cttcaaaagt aaatggatag
    14341 tagaatggtg ttacggcaga aagtgcatca taaccgagtt ctgtggcata ttttccaagt
    14401 tcaatggctt catttaaatc taacgaacct acttgagcaa tcaatttcac tttatcccca
    14461 actgcctctt tggcaacctt gaaaacttgc ttcttctgct ctgtatttaa taaaaagttt
    14521 tcgcctgagc taccatttac ataaagaccg tctaattctt cagtttcaat ggcattttga
    14581 gcaatttgtt taagtccttg ttcatttact tgaccatttt catcaaaagg aacgagtaac
    14641 gctgcatata aaccttttaa atctttgttc attatgaagt ccctccaaaa atcatttgat
    14701 aatatagttt acagctataa ttgtaaacgc tatcataaaa tgtaacaata tctttttgaa
    14761 aattgtagtc atatttatgt ataattaatg aaaatgtttt tcaaaatcaa tagaaatgga
    14821 gtgagtaagg tgtattacat cgcaatcgat attggaggca ctcaaattaa atcggcagtt
    14881 attgataagc aattgaatat gtttgactat caacaaatat caacgccgga caacaaaagt
    14941 gagcttatta ctgacaaagt atatgagatt gtaacaggat atatgaagca atatcagttg
    15001 atccaacctg tcataggtat ttcatcagca ggcgttgttg atgaacaaaa aggcgaaatt
    15061 gtatacgcag ggccaaccat tccgaattat aaaggtacta attttaagcg attattaaaa
    15121 tcactgtctc cttatgtcaa agtaaaaaat gatgtaaacg ctgcattact aggcgaattg
    15181 aaattacatc aatatcaagc agaacggatc ttttgtatga cgcttggtac aggcattggg
    15241 ggtgcgtaca agaataatca aggtcatatt gataatggtg agcttcataa ggcaaatgaa
    15301 gttgggtatt tattgtatcg tccaactgaa aatacaacgt ttgagcaacg tgctgcaacg
    15361 agtgcattga aaaagcgcat gattgccgga ggatttacga gaagcacaca tgtgccagta
    15421 ttgtttgaag cagctgaaga aggtgatgat attgcaaaac aaatattgaa tgagtgggca
    15481 gaagatgtag cagaagggat tgcccaaata caggtcatgt atgatccagg gcttatatta
    15541 attgggggcg gtatatctga acaaggagat aatctcatta aatatatcga gccgaaagtt
    15601 gcacactatt taccaaaaga ctatgtttat gcaccaatac aaacgactaa gagtaaaaat
    15661 gatgcagcat tatatggctg tttgcaatga tagttgaaag aaggagtcat tctaaaatag
    15721 aatttgaaac cgttacgaga gatgagagct gttgttagtt ccacacatca cactctatct
    15781 aggaccaatc taaactatat caaccaacag tgtgccacgg gcaaattaaa ttgaagaagc
    15841 tgagatatta aaattttaga aaatgtaaaa aaatatttgg tattgaaatt aaaaaagcac
    15901 ctagcaactc gttgggacaa tcacgatgat tgtctacagt tgcaggtgga tttgaatata
    15961 ctactagtta tttgttgtct aggataatag atttagtatg ttgataagtt tgactcagat
    16021 tcgtattttc taataaatga taactcacga tatcgattaa aaagagtgtc gcaatttgtg
    16081 tgttgataaa ttgatggtcg gtattacgcg attgatccgt tgttaaaagt actaaatctg
    16141 cacaatctgt aagtttacta ccttcaaaat ttgtgatggc aacgacatat gcaccatgag
    16201 atttggcgac ttccgctgca gaaattaatt ccgaagtatt accactattt gacatagcaa
    16261 taaacatatc cgaatgagat agtagggatg ccgatatttt cattaaatgt gaatcggtag
    16321 taacattacc ttttagcccc atacgaatca tacgataata aaattcagtc gctgataaac
    16381 cagagctacc tagtccagca aagagtatat gtcgacttga ttgaagtttg tcgataaagg
    16441 tttggataat gtcgttatca ataaattcac cagtttgttg aatgatttgt tgatgatatt
    16501 tatgaattct ttgaataatt gggctatttt caataactgt ctctgtcatt tcttgttgaa
    16561 tattaaattt taaatcttgg aaattctcat aatccagctt atgactaaag cgtgtcatcg
    16621 ttgctggtga tgtaccaatc gcatgggcta aggagttaat cgttgaaaag gcatcgctat
    16681 aaccattttg tcttatataa ttgacgatgc gtttatcagt ttttgtaaat aaatgttgat
    16741 aacgttgaac acgattctca aatttcattg tgtcacccct tcatcttaat gattactatt
    16801 atatatgaaa aatattttca agatagtaaa aagcattgat aaaaattatc ttaatgatat
    16861 attgtaaatg actttacgtg aaaaacgact tatggagtga ggaataatgt taccacatgg
    16921 attaatagta tcttgtcagg cactaccaga tgaaccattg cattcatctt ttattatgtc
    16981 gaaaatggca ttagctgcgt atgaaggtgg tgctgttggt attcgcgcaa atactaagga
    17041 agacatttta gcaattaaag aaacggtaga tttaccagtt attggcattg tgaaacgtga
    17101 ctatgatcac tcagatgttt tcattactgc aacgtcaaaa gaagttgatg aactgataga
    17161 aagccaatgt gaagtcattg cattggatgc aacgttacag caacgtccga aagaaacgtt
    17221 agacgaatta gtatcatata ttagaacaca tgcaccgaac gttgaaatca tggctgatat
    17281 cgcgaccgtt gaagaagcta aaaatgccgc acgacttggc tttgattata ttggcacgac
    17341 gttacatggc tatactagtt atacgcaagg acaattactt tatcaaaatg acttccaatt
    17401 tttaaaagat gtactacaaa gtgttgatgc aaaagttatt gcggaaggta atgtcattac
    17461 accggatatg tataaacgtg tgatggactt aggcgttcat tgttcagtcg ttggtggtgc
    17521 gataacacga ccaaaagaaa ttacgaaacg ttttgttcaa attatggaag attaaatgat
    17581 aacgataaaa aaacgagatg accatcatta attaaaggca cctaattatc ttaggtggct
    17641 gaatgaatgt aatgggttca tctcgttttg tttgtttatg atagtgattt tattttcaac
    17701 tttatccaaa aataagtaaa gcgacgggga tggtgattaa tagcgacaac gccacgcgta
    17761 aaaaccaaat gatgatgagt ttccagacag gtattttaat ttcagttgct agtatacatg
    17821 gcactaatgc tgagaaaaag ataatggctg atacgcttac tacaccgacg acaaatttag
    17881 tactcattgc agctttagtt actaacaaag atggtagaaa catctctaca atagaaatcg
    17941 ctgacgcttt tgctagtaaa gcctgatcag caattgggaa aatataaata aatggataga
    18001 agatatagcc aagccaatca atgaatggtg tatagttcgc tacaatcagt cctaaaaaac
    18061 caatcgataa tatagaaggt aaaataccaa cagtcatttc taaaccgtct ttcaaattgt
    18121 cccaaacgtt cttcacgaga gatggtgtta atgcattttg tttcatcgcc tctgcatatg
    18181 cagttttcag tctgcttcct tcaatagcaa cttcttgttc tccttcttgt ccgttataat
    18241 attctgttga ttcattgctg attggcggta gccatgcagt aattgcagtc acgacaaatg
    18301 tgatgactaa agttatccaa aagtataaat tccaatgcgg cattaatcct aaagttttag
    18361 caacgataat cataaaagtt gctgaaactg ttgaaaagcc agtcgcaata atcgtggctt
    18421 ctcgtttgtt gtacatccct tgcttataga cacgattagt aatcaataat cctaaggaat
    18481 aactgccgac aaacgaagcc actgcatcga cagcggattt tcctggtgtt ttaaaaatag
    18541 gtctcataat aggctccata taaacaccga caaattctaa taagccatag cccactaata
    18601 aagaaagcgc aattgcacct actggaatta agatacttaa tggcatcatt aatttttcaa
    18661 acaaaaacgg accatagtta gctttaaata gtattgatgg accgatttta aatacataca
    18721 ttataccgat cattgcacct gcaactttaa ataatgtaat gaccaagttt gtgattgaag
    18781 tcataaaagt acgtctcact attggtaacg ctgtaccaat taaaatcata atcagtgcaa
    18841 catagggcat aagtggacct atgattgagc gaatggctag atgaacatga tcgacgaaaa
    18901 tagtgttgtt accattaatc gtaaaaggaa taaagaaaca tagtatgccc actaaactat
    18961 agacaaaaaa acgccatgca cttggttgtt gtgcattaga atgatattga ttcattaaag
    19021 caaccccttt gtttaaatga atacacaaaa ctgtatgatg catcttcccc ttaatgagat
    19081 gaatcattat tttaatttag aaaaatctga aaacttacta taattgtata gtttgaatta
    19141 ttttcatacc aatacaaatt aactaatt
//
LOCUS       DAKTLH010000037        19092 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.037, whole genome shotgun sequence.
ACCESSION   DAKTLH010000037 DAKTLH010000000
VERSION     DAKTLH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19092
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.037"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            29..1723
                     /gene="ezrA"
                     /locus_tag="O0394_001110"
     CDS             29..1723
                     /gene="ezrA"
                     /locus_tag="O0394_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCY0499199.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(1797..1937)
                     /locus_tag="O0394_001111"
     CDS             complement(1797..1937)
                     /locus_tag="O0394_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499200.1"
                     /translation="MIMLFSLLILIKTTHLITFIFKQLLFIFIVPFIYPYKKEARQRI
                     VG"
     gene            2086..3234
                     /locus_tag="O0394_001112"
     CDS             2086..3234
                     /locus_tag="O0394_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCY0499201.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKTQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            3234..4457
                     /gene="thiI"
                     /locus_tag="O0394_001113"
     CDS             3234..4457
                     /gene="thiI"
                     /locus_tag="O0394_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCY0499202.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAISAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGAVLKHFDNISVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            4501..5271
                     /locus_tag="O0394_001114"
     CDS             4501..5271
                     /locus_tag="O0394_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HCY0499203.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSTIKFIRSGKVDLYVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAIIFAALFILIGFYDGFVGGGTGS
                     FMLFVLLVFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMAISMIAGSY
                     AGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            5369..5863
                     /gene="tpx"
                     /locus_tag="O0394_001115"
     CDS             5369..5863
                     /gene="tpx"
                     /locus_tag="O0394_001115"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCY0499204.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSDASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            5986..6933
                     /locus_tag="O0394_001116"
     CDS             5986..6933
                     /locus_tag="O0394_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0499205.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKIPSQFQGFMTELNQWMDTNRPKK"
     gene            7021..8223
                     /locus_tag="O0394_001117"
     CDS             7021..8223
                     /locus_tag="O0394_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCY0499206.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
     gene            complement(8469..8969)
                     /locus_tag="O0394_001118"
     CDS             complement(8469..8969)
                     /locus_tag="O0394_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCY0499207.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            9110..10228
                     /gene="ald"
                     /locus_tag="O0394_001119"
     CDS             9110..10228
                     /gene="ald"
                     /locus_tag="O0394_001119"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCY0499208.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            10352..10450
                     /locus_tag="O0394_001120"
     CDS             10352..10450
                     /locus_tag="O0394_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499209.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLG"
     gene            complement(10544..11599)
                     /locus_tag="O0394_001121"
     CDS             complement(10544..11599)
                     /locus_tag="O0394_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCY0499210.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTECEVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            11774..12463
                     /locus_tag="O0394_001122"
     CDS             11774..12463
                     /locus_tag="O0394_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCY0499211.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(12768..13181)
                     /locus_tag="O0394_001123"
     CDS             complement(12768..13181)
                     /locus_tag="O0394_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCY0499212.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            13386..14684
                     /locus_tag="O0394_001124"
     CDS             13386..14684
                     /locus_tag="O0394_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCY0499213.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            14945..15886
                     /gene="pde2"
                     /locus_tag="O0394_001125"
     CDS             14945..15886
                     /gene="pde2"
                     /locus_tag="O0394_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HCY0499214.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGNEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVDSWDEFEQLATALRTKLN"
     gene            15907..>19092
                     /locus_tag="O0394_001126"
     CDS             15907..>19092
                     /locus_tag="O0394_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCY0499215.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKKVGDQHRDIVQVFETHNDTYMDHLSISIQGRKHVWIQN
                     VCYQTRQDADTISALAAIRDNTKLDLIHDQEDFGAHFLTEKEINQLDINQEYLTQVDV
                     IAQKCDAELKYHQSLLPQYETPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNYKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYINQKDSMFNNFIQSFNPSDI"
BASE COUNT         6714 a         2669 c         3546 g         6163 t
ORIGIN      
        1 agtaatgata aattaggagg agaagcatat ggtgttatat atcattttgg caataattgt
       61 gattatattg attgctgtag gtgtattatt ctatttacgt tcaaataaac gacaaattat
      121 tgaaaaagca atcgaacgta aaaatgaaat tgagacgtta ccttttgatc aaaaccttgc
      181 acaattatct aagttgaatt taaaaggtga aacaaaaacg aaatacgatg caatgaaaaa
      241 ggacaacgta gaaagtacaa ataagtatct agctcctgtg gaagaaaaaa tccataatgc
      301 tgaggcttta ttagataaat ttagtttcaa cgcatctcaa agtgaaattg atgatgcaaa
      361 tgagttgatg gatagttacg aacaaagcta tcagcaacaa ttagaagatg taaatgaaat
      421 tattgcgtta tacaaagata atgatgaatt atatgacaaa tgtaaggttg attatcgtga
      481 aatgaaacgt gatgttttag caaatcgtca tcaatttggt gaggcagcaa gtctacttga
      541 aactgaaatt gaaaaatttg agccaaggtt agagcaatat gaagtactaa aagctgatgg
      601 taattatgta caagcgcaca accatatagc tgccttgaat gaacaaatga aacagctaag
      661 atcttatatg gaagaaatac cagaattaat tagagaaact caaaaagaat tacctggtca
      721 attccaagat ttaaaatatg gttgccgtga tcttaaagtt gaagggtatg atctggatca
      781 cgtgaaagta gacagtacat tacaaagctt aaaaacagag cttagtttcg ttgaaccatt
      841 aattagccgc ttagaattag aagaagctaa tgataaacta gctaatatca atgataagtt
      901 agatgacatg tatgatttaa ttgaacatga agttaaagct aaaaatgatg tcgaagaaac
      961 aaaagatatc attacggata acttattcaa agctaaagac atgaattata cattgcaaac
     1021 agaaattgaa tatgtacgtg aaaactacta tataaatgaa tctgatgctc agagtgttcg
     1081 tcaatttgaa aatgaaattc aaagtttaat ttctgtatat gatgatattt taaaagaaat
     1141 gtctaaatct gctgtgcgat atagcgaggt tcaggataat ttacaatatt tagaagatca
     1201 tgtcacagtt attaatgaca aacaagaaaa gctacaaaat catctgattc aattgcgtga
     1261 agatgaagca gaagcagaag acaatctgct acgagtacaa tcgaagaaag aagaagtgta
     1321 tcgtcgatta cttgcttcta acttaacaag cgttcctgaa aggtttatca tcatgaaaaa
     1381 tgaaattgat catgaagttc gtgatgttaa cgaacaattt agtgaacgtc caatacacgt
     1441 taaacagtta aaagataaag tgtctaaaat tgtgattcaa atgaatacat ttgaagatga
     1501 agcaaatgat gttcttgtta atgctgttta tgcagagaaa ttaattcaat atggaaatag
     1561 atatcgtaag gactatagca atgttgataa gagcttaaat gaagctgaac gattatttaa
     1621 aaataatcgc tataagcgtg cgattgaaat tgcagagcaa gctcttgaaa gtgttgagcc
     1681 aggtgttact aaacatattg aagaagaagt tattaagcaa tagaaactag tatgtagtta
     1741 tacttaaata atatgagcac tctgtcaaat tggactgatg agtttaataa ttgaagttag
     1801 ccaacgatac gttgtctagc ttctttttta tatggataaa tgaaagggac aataaatata
     1861 aatagcaatt gtttaaagat aaacgtaatc aaatgtgttg ttttaattaa tataagtagt
     1921 gaaaaaagca taatcacaca gctgtttaaa tagagtgaaa tagtctaatt cttatttaat
     1981 aagtagaaat aagattattt tgcgctataa cctttatttt ttgtcggata atagtataat
     2041 gcttgagtaa aacaatttta acgattgatg attaatgaaa gagaagtgtt tattttgata
     2101 tatctagata atgcggcaac gacgaaagca tttgaagaag tgttagatac ttatttaaaa
     2161 gtaaatcaat caatgtatta taatccgaat agtccgcata aagctggttt gcaggcaaat
     2221 caattactac aacaagcaaa aacccaaatt aatgcaatga ttaattcaaa aacaaattat
     2281 gatgttgtat tcactagtgg tgcaactgaa tccaataatc ttgctttaaa aggtattgcc
     2341 tatcgtaaat ttgatacagc gaaggaaata attacatccg tgttagagca tccgtccgta
     2401 ttagaggttg taagatattt ggaagcacac gaaggattta aagttaaata tgttgatgta
     2461 aagaaagatg gcagtattaa cttagaacac ttcaaagaat taatgtcaga caaagtcggt
     2521 ttagtaacat gtatgtatgt aaataatgta actggacaaa tacagcctat tccacaaatg
     2581 gctaaagtta taaaaaatta tcctaaggca cattttcatg tagatgcggt tcaagcattc
     2641 ggcaaaattt caatggatct caataacata gatagtatta gtttaagtgg acacaagttt
     2701 aatggtttaa aaggacaagg cgtcttactt gtaaatcaca ttcaaaatgt tgaaccaact
     2761 gtccatggtg gtggtcaaga atatggcgtt agaagtggaa cagttaattt gccaaatgat
     2821 attgcaatgg ttaaagcgat gaagatagct aatgaaaact ttgaagcatt gaatgcattt
     2881 gttactgagt taaataatga cgtccgtcaa tttttaaata aatatcatgg agtttatatt
     2941 aattcttcaa cttcaggttc accattcgtt ttaaatatta gttttcctgg cgtaaaaggt
     3001 gaagtattag ttaatgcttt ttcaaaatat gacattatga tatctacgac aagtgcttgt
     3061 tcatctaaac gtaataaatt aaatgaagta ttggctgcaa tgggattatc agacaaatct
     3121 attgaaggta gtataagatt atcatttggg gctactacaa ctaaagaaga tatagcgagg
     3181 tttaaagaaa tatttatcat catttatgag gaaattaagg agttgctaaa ataatgaagt
     3241 atgatcactt gcttgttaga tacggggagt taacattaaa gggttcaaat agaaagaaat
     3301 ttgtaaatca attaagaaat aatgtaaata agtcattaaa aggacttgat gggtttgtcg
     3361 ttaaaggcaa acgagatcgt atgtatattg aacttgaaga ccatgcagat ataaatgaaa
     3421 taacatatcg attatcaaaa attttcggta ttaaatctat tagtccagta ttaaaagtag
     3481 aaaaaacaat agaggcaata agtgcagcgg caattaaatt tgcgcagcaa tttgaagaaa
     3541 acagcacatt taaaattgat gtgaagcgtg ccgataaaaa tttcccaatg gatacgtatg
     3601 aattacagcg tgaattgggt ggtgcagtat tgaagcactt cgacaatatt tcagtgaatg
     3661 tcaaacgtcc agatcatgaa attcgagtgg aagttagatt agatgcaatt tatatgtatg
     3721 aagaagttgt tccgggttca ggtggattac cagttggtac tggtggtaag acgttactaa
     3781 tgctttcagg cggtatagac tcaccagttg ctgggatgga agtgatgaga cgtggcgtaa
     3841 caattgaagc gattcatttc catagtccac catttacaag tgatcaagca aaagaaaaag
     3901 ttattgaatt gacacgtatt ttagctgaac gtgttggacc aattaaattg catattgtac
     3961 catttacaga attgcaaaaa caggtaaata aagttgtaca tccaagatat acaatgactt
     4021 caacgagacg tatgatgatg cgtgttgctg ataaattagt acatcaaata ggggctttag
     4081 ctattgtaaa tggtgaaaac ctagggcagg tagccagtca aacacttcat agcatgtatg
     4141 caattaataa tgtaacttct actcctgtat tacgtccttt attaacttac gataaagaag
     4201 aaattattat taaatcgaaa gaaattggta catttgaaac atctattcaa ccatttgaag
     4261 attgttgtac aattttcacc cctaaaaatc cagtaaccga accaaacttt gataaggtag
     4321 tccaatatga aagtgtcttt gattttgaag agatgattaa tcgtgctgtt gaaaatattg
     4381 aaacacttga aataactagt gattataaaa ctattaaaga acagcaaaca aaccaattaa
     4441 taaacgactt tttataaata aaatcctaga gtaaatttaa acataagggg atgttaaact
     4501 atggatttga acttaacgat gattataatc ataattttat ttggttttat cgcggcgttt
     4561 atagattcgg ttgtaggggg tggcggttta atttctacgc cagcattatt agcaatcggt
     4621 ctaccaccat ctgtggcttt aggtacaaat aaattggcaa gttcgtttgg ttctttaact
     4681 agtacgataa agtttataag gtccggtaaa gtggacttat atgttgttgc caaattattt
     4741 ggttttgtat ttttggcatc tgcatgtggc gcatatattg caacgatggt tccgtcacaa
     4801 atattgaaac ctttaatcat cattgcactt tcgtcggtgt ttatattcac attacttaaa
     4861 aaagattggg gcaatacacg cacgtttact caatttacat ttaagaaagc cataatattt
     4921 gcagcacttt ttatattaat cggcttttat gatggatttg taggtggggg aacaggttca
     4981 tttatgcttt ttgtattgtt agtctttggt tttgattttt taagtgcagc aggaaatgct
     5041 aaagttttga actttgcttc taatataggt gcgcttgtat tatttatggt attaggacaa
     5101 gtagattatg taataggttt aattatggct ataagtatga ttgctgggtc atatgctggg
     5161 gctcattttg ctattaaaca aggtgttggt tatgtaaaag tattatttat tattattact
     5221 gctattttaa ttttaaaaaa tgcttttgat tatattcaac aatttgtata aaattaaatt
     5281 tgcttttgat taaagcatga aaattgtaat caaaccataa attgtcgtat gatgtagtta
     5341 gaattttaaa atgcaggagg tcaagtatat gactgaaata acattcaaag gtggaccaat
     5401 ccacttaaaa ggtcaacaaa ttaatgaagg tgattttgca cctgatttta cagtgttaga
     5461 taatgactta aatcaagtaa cattagcaga ttatgctggt aaaaagaaat taattagtgt
     5521 ggtaccatca attgatacag gtgtttgtga tcagcagact cgcaaattca actctgatgc
     5581 ttctaaagaa gaggggattg tgcttacaat ttcagcagac ttaccattcg cacaaaaaag
     5641 atggtgcgct tcagcaggtt tagacaatgt cattacatta agtgaccacc gtgacttatc
     5701 atttggtgaa aactatggcg ttgttatgga agaacttcgc ttattagctc gtgcagtatt
     5761 tgtattagat gcagataata aagttgttta taaagaaatc gttagtgaag gtactgattt
     5821 cccagatttt gatgctgctt tagctgcata caaaaatatt taatcattaa agagataaat
     5881 cttaaaatgt atacatcgtg tccatcgttg tcaacagcat taaaatagaa ttgttttcta
     5941 tgattgctaa gacctatggg cactttttat tggagaggga cgaatatggc agaacaacaa
     6001 acaattatgg aacgcttgtt tcatacatta gatgaaaaag ctaaaacatt aaataatgaa
     6061 aatggccaaa gttttattga aaatcttggg ctagcaatgg aacaagtata taccaatgaa
     6121 agaggattgt tagaacaatc gacgttacaa gatcgacgta aagcattcca atttgcatat
     6181 ttaagtttaa tgcaggaaga aaagatacaa gcaaatcatc aaattacacc agattcaatt
     6241 ggattgatac taggattttt agttgagcgt tttatgaaca accaagaaga attacatatt
     6301 gttgatattg caagtggtgc cggtcattta agtgctactg taaaagaagt gttacctgaa
     6361 attgcggtta tgcatcattt aattgaagtt gatccagttt tatcacgtgt tagtgtacat
     6421 ttagcaaact tcttagaaat tcctttcgat gtgtatcctc aagatgccat catgccacta
     6481 ccattagaag aagcagatat cgttattggt gattttccag taggctatta tccaattgat
     6541 gaaagaagta aggagtttaa gctaggtttt gaagaaggac atagttattc acattattta
     6601 ttaatagaac aagcaataaa tgcattaaaa gatgctggat atgcctttct agtggtacca
     6661 agtaatattt ttacaggtga acatgtaaaa cagcttgaaa aatatattgc aacagagaca
     6721 gagatgcaag catttttaaa tttaccacca actttattta aaaatgaaaa agcgcgaaaa
     6781 tctatattaa ttttacaaaa gaaaaaatcg ggtgaaacaa agccagttga agtattattg
     6841 gcaaatattc ctgatttcaa aattccttca caatttcaag gatttatgac agagttaaat
     6901 cagtggatgg acacaaatcg tcctaaaaaa taatctgtaa tagtatagtc ataaaactgt
     6961 atgaatgata aaatgaaaaa tgaaatagta taaaaagcaa acgatggagg atcattactt
     7021 atgtcaaaat taatcttggc tatcaatgct ggtagttcat cattaaaatt tcaattaatt
     7081 agaatgcctg aagaggaatt agtaacaaaa ggtttagtag aaagaatcgg attgaaggat
     7141 tcaattttta caattgaagt caacggggaa aaagttaaaa cagtacaaga tatcaaggat
     7201 cacgttgagg cagttgatat catgttggat gcttttaaag cgcacaatat tattaatgat
     7261 attaacgata tcgatggaac aggtcaccgt gttgtacatg gtggtgaaaa attccctgaa
     7321 tcagtagcta ttactgatga ggttgaaaag gaaattgaag aattaagcga attagcaccg
     7381 cttcacaacc cagctaactt gatgggtatt cgtgctttcc gtaaattatt accaaatatc
     7441 cctcatgtgg caatttttga tacagcattc catcaaacaa tgcctgaaaa agcatatcta
     7501 tatagcttgc catatcatta ttataaagat tatggcattc gtaagtatgg tttccatggt
     7561 acaagccata aatttgtatc acaaagagcg gcagaaatgt tagataagcc aattgaagat
     7621 ttacgtatta tttcatgtca cattggtaat ggagcatcaa ttgcagctat cgatggcggt
     7681 aaatctattg atacatcaat gggcttcaca ccacttgctg gtgtaacaat gggtactcgt
     7741 tctggtaata ttgaccctgc tttaattcca tttattatgg aaaaaacagg taaaactgct
     7801 gaacaagtat tggaaatctt aaacaaagaa tcaggtttat taggattatc aggtacatct
     7861 agtgatttac gtgacttatc agaagaagct gaatcaggta aagcacgttc acaaatggct
     7921 ttagatgtat ttgcttctaa aattcacaaa tatattggat catatgctgc gagaatgcat
     7981 ggtgttgatg ttatcgtatt tacagcaggt atcggtgaaa actcagtaga aattcgtgcg
     8041 aaagttcttg aaggcttaga atttatgggt gtttactggg atcctaagaa aaatgaaaac
     8101 ttactacgtg gtaaagaagg atttattaac tatcctcatt caccagttaa agttgtcgtt
     8161 attccaacag atgaagaaag tatgattgcc cgtgatgtta tgacattcgg tggtttaaaa
     8221 taatttttaa ataaatatta agcgtctatc tccgctacaa ttatatgtag gaggtagacg
     8281 ctttttgtat tgtttgaaaa ttaactagtg taatgtctct tttcacgaat atcttaaaga
     8341 attaagtagt cgaagaagat tatgaaaaca ttattaagtg agttttattt ataaacaata
     8401 aaaaaccaag tgcagaaagt tgcacttggt tcataatgat caagtagtta tctaatgatc
     8461 atctatattt aattttgata tttttcacgt aattgagttg ttgcaacttg tggttggaag
     8521 tctgctggta actcttcagt acgaacaact aacacgtcac atggcgcatg acgaacgata
     8581 gattctgata cagaaccaac aataaatctt tccacggcat ttaagcctga tgtaccactc
     8641 ataatcaagt ctgcattaat ttcatgtgca agctttttag gaatgataga tttaggagag
     8701 ccaaactcta gacgcgtttc tacatcttta acaccagcgt tagtagctac ttctttataa
     8761 ccatttaata attcttctgc aaaatgctta gatttttcag taaattgagc atcataaact
     8821 tcataagaag aatacgttct tgaatcaatt acattcacaa ttgttaactt cgcatcgtta
     8881 cgtttagcaa caccaactgc tctgttaaat gcccattccg cttcatgtga accgtcaact
     8941 gcgattaaaa tatttttgta agtaatcata gtaagtgcct ccttgttctt tcttaattat
     9001 attttaccac aattttcgga aaagtcagtg taataacctt tacaattttg tggatttttg
     9061 attgcgcttt caaaatgttc gcgttaagat gattatggag gtggatggta tgaaaattgg
     9121 tataccaagg gagattaaaa ataatgaaaa tcgtgttggt ttatcaccaa gtggtgtgca
     9181 cgctttagta gaaagtgggc atactgtttt agtggaaaca aatgcgggtt caggatcatt
     9241 ctttgaagat gtagattaca aagaagcagg tgctgagatt gttgctgaac aagcaaaagt
     9301 ttgggatgtg gatatggtta ttaaagttaa agaaccactt gaatctgaat atccatattt
     9361 taaagaaggg cttgtattat tcacttatct tcatttagca aatgaagaaa aattaacaca
     9421 agctttgata gatagaaaag taattagtat tgcatatgag actgtgcagt taccagaccg
     9481 atctttacca ttgttatcac caatgagtga ggtagcagga agaatgtcag ctcaagttgg
     9541 cgcagagttc ctacaaaaac ttaatggtgg tatgggaatt ctactaggtg gtgtcccagg
     9601 agtacctaag ggtaaagtaa ctattatcgg tggtggtcaa gcaggaacaa atgcagctaa
     9661 aattgcacta ggactaggtg cagatgttac gattttagat gttaatccaa agcgtttaca
     9721 acaattagat gatttattcg gtggacgtgt acatacaatt atgtcaaatc cgttgaatat
     9781 tgagttgtat gttaaacaaa gtgatttagt aataggtgca gttttaattc caggtgctaa
     9841 agcgccaaga cttgtaacag aagacatgat taaacaaatg aaaaatgggt cagttattat
     9901 tgacattgct attgatcaag gcggtatttt tgaaacaact gataaaatta cgacacatga
     9961 tgatcctaca tatattaagc atggtgtggt tcattatgca gttgcaaata tgccaggtgc
    10021 agtaccgcgt acttcgacgt tagctttaaa taatgctacg ctaccttatg cgctcatgct
    10081 agctaataaa gggtatagag aagcatttaa atcaaatcaa ccattatcat taggtttaaa
    10141 tacttacaaa ggtcacgtaa ccaataaagg cgttgcagag gcatttgaaa tggaatataa
    10201 atctgtagaa gaagcattac aattataatt tgaatttgat gaaaagggaa tggattacag
    10261 cgtactaaag aatgacgtta tcatctcctt ggttattaaa attaaataat gataaattaa
    10321 agctagaaac aatgcgaaga aatcatcgaa catgaatgat attgatttga ttcattacat
    10381 gttactgatg aacatacctg catatgtgtt tctagcttgt ttgttacata ttgcatttag
    10441 acttggataa aaatttatgt cttaggcgct acaatgttat attggcagtg tttgactgaa
    10501 tgagaatgcg cttgtaacaa gcttttcatt tttatcccac tccttatttt tcgtaatgtg
    10561 ttaatacttc atatccttca ttagtgacaa gtatgtcatc ttcaattctt acacctgcaa
    10621 caccaggtac ataaatacct ggttcgattg taataaccat gccagcttct aacaaattag
    10681 aattagtact tgaaacatct tgatattcat gttcttgtaa tcctaggcca tgacctaagc
    10741 gatgagggaa atattcacca taaccttttt ctgaaataat atttctagcg atatgatcga
    10801 tatcttttaa tggtattcca ggtttaattg cttggattgc agatgtttct gcttctaata
    10861 caatattata aatttcttgt gcttctttgc taggttcacc aaatttaata gtacgtgtca
    10921 tatcgctaca ataatgctca taaattacac ctaaatcaaa tagtacatat tcattgcttt
    10981 ttaatctgcg atctcctggt gtgccatgag gtgatgcggc atgatctcca aataaaacca
    11041 tcgtatcaaa actcatttca ttgacgccat attgtttgat agtttgctca atatggttga
    11101 ctacttcaca ttcagtcaca ccttctttta aataagaaac acctatttcg atacacttat
    11161 ctgctaactc agcagcttta cgtattttgc taatttcatc ttcggattta atatttctca
    11221 attgtttgat tgttaaatca acatctccga atgaattgac attgaaacca gagattaatt
    11281 gtttttggcg tgctactgtt aagtgctcgc tttcaattag taatttattg attgtttgag
    11341 gataaagtga aaaagggttt tcagtgtcta aatatccaac gatttcacct gtgaaaggtg
    11401 atgctttgac ttcttcgact tccatttttg gacaaaatag tacttgttta ccatctttct
    11461 taatcaataa tgcaaataat ctttcatggg gttcgctacg gtatccagta aaataatata
    11521 cattcaacgg tgttgtaatc catgctgcat cagcttgttg attgttcaat tcgtctatta
    11581 tttttgatat ttttgtcatt ttataaacac tcctcaacat aatattactt ccattttatg
    11641 ccaattatta ttagatttca aaatcgagct aatacattaa ctagattttc aatttttaaa
    11701 atttgagaat ttgttataat taagtggcaa aagggtacta aatttatata gtaattgata
    11761 ggaggatgct caaatgaagt tatcatttca tggtcaatca acgatttatt tagaagggaa
    11821 caacaaaaag gttatcgtag atccgtttat ttcaaataac cctaagtgtg atttgaatat
    11881 tgaaacagtg caggtagact atatcgtttt aacgcacggt cattttgatc attttggtga
    11941 tgttgtggaa ctagcaaaga aaacaggagc aacagtaatt ggaagtgcag aaatggctga
    12001 ctatctttct tcatatcacg gtgttgaaaa tgttcatggt atgaatattg gtggtaaagc
    12061 aaactttgat ttcggaagcg ttaaatttgt tcaagcattt catagttcaa gttttacaca
    12121 tgaaaatggt attccagttt atttaggtat gccaatgggt atcgtgtttg aagttgaagg
    12181 aaaaacaatt tatcatactg gtgatacagg tctgtttagt gacatgtcac ttattgcgaa
    12241 acgtcatcca gttgatgtat gttttgtacc aattggagat aattttacga tgggtattga
    12301 tgatgcaagt tatgctatta atgaatttat taaaccaaaa atttctgtac cgattcatta
    12361 tgatacattc ccattaatcg aacaagatcc acaacaattt aaagatgctg ttaatgtagg
    12421 ggacgtgcaa attttaaaac caggtgaatc tgttcagttt taaatagaat atagatttta
    12481 cactaaaaaa cacctcgcta atctatgtgg aagttatcgt ctgcatattt ttagtgggtg
    12541 tttttatatt ctactgattc agttacgacg tggacggggg acatcagtgg ttctttatca
    12601 tttctgacct actctcgtaa ttttagtttt atacaattag acattgatta taaatttact
    12661 acgatataaa aaatccagaa ccacagtcat aatcgtttct tgtatttact cagtcgtttg
    12721 agactgcatc atgattgaaa gattatttgt gattctggat aaaaatttta tttaacgatt
    12781 aatacaggga tagtcgcacg ttttgctact ttgtggctga cacttccaag tacaaatttc
    12841 tttttgtctt ctgctttacg gttacttaaa acaacaattt cgtatttacc gctattagca
    12901 tgttttaata gttcttcttt tgcattacca cgaacaatta tttggtcata atcaatacca
    12961 taatcttcta aagctttgcg tgttttttcc aatcgcttgc ttcgttcttc agtaagtttg
    13021 tttaaatgaa cacctgcttt aattgatgct tgagcatctt gttcgctgat tgcgtttaaa
    13081 actgttacga ctgtaccttc gccagctaat ttagacactt cttttagtgc tttttcattt
    13141 tttaactgag tgtctacacc aagtaatata tttttataca ttagtacatc cctcctcact
    13201 tacattctaa taaataataa tggatttttc catttaaaat taaagatttt gtgatctttg
    13261 tgtttgttgt atattttcaa aaaataaaat aggtgaaacg atcattattt catttcactt
    13321 caatgctttt tatccaaaat aaagttataa tgtagttaat actgttcgaa aagaagaggt
    13381 aaagaatgac aaaacatgaa caaattttag attatattga atcgttatct ataggctcta
    13441 aaatttcggt gcgtaaaatc gctaaatttt taaacgtatc tgaagggaca gcatatcgtg
    13501 caattaaaga tgctgataaa atgggcatgg tcgcaacgat agatcgtgtt ggtacggttc
    13561 gaatcgaaaa acgtaatcgt aatgaaattg aacatttaac ttttaatgaa attgtcaata
    13621 ttattgatgg ccaagtgtta ggtggcaaca aaggtatcac taagatggta tctaaatttg
    13681 caattggagc aatggaactc aaagatattt taaagtatat tggtccaaag acattattga
    13741 ttgtcggaaa tcgtgaagat gtgcagattg aagctttaaa aagaggtacg gctatattaa
    13801 ttactggtgg atttaagcca tctaataaag ttattaattt tgcgaatgaa catgatttac
    13861 ctgttctatc atcaagttat gatacattcc tagttgcaaa tattatcaac aaagcacttt
    13921 ttaatcagaa aatacgaaaa gatatcttaa tagttcaaga tataatgacg ccattagatg
    13981 atttatctgt gctgtttgat acgatgaaaa tagcagatta taagcgtatg gcgaatcgaa
    14041 ctggccatac aagatttccg gtagtcaatg agtcgtataa attagtaggt attgtaacaa
    14101 gtcgagagat gattaataca aaggacgacg acgaaataga taaagtgatg acgcgaaatc
    14161 ctatttatgt aaatgctatg agtacagtag caagttgtgc gcatatgatg atttgggaag
    14221 gtattgaatt gattcctgtc gtttcgagta ataaaaagac agtgggtgtg attaatagac
    14281 aagatgtttt aaagtctatg caacttttag gaagacagcc tcagatgggt gaaacaatta
    14341 acgatcaaat cgccaaatat ataacaatga atcaagatgg aattacagta gaagtttcac
    14401 cacttttaat caatcattac ggaacagtaa gtaaagcggc atttgtaagt attatagaag
    14461 aaacaataca atatgaaatg cgtaaattta aaaaaggtaa tgtcatgata gagaacttaa
    14521 atatcgtgta tattaaaaca gtacctattg aatcacatat aacagtgcgc tttggcatac
    14581 tagacgtcgg aagaaacttc gccaaaatag aagtgaacat gcatagtcaa aacgacaaag
    14641 tagcgagcgc tctcgtgata tgccaaatgt ttgacgaagt ataggaactt agcagagaaa
    14701 ataggcataa gatagatttc gcacaaacgt tggggcccca ccccaacttg cattgtttgt
    14761 agaaattagg ggtccaattt ctctgtgttg gggcccctga actaaatagt atgaaaatat
    14821 atatatctaa ataattaaaa cgggcaaata gtttaatatg gtagtaacaa tttttataat
    14881 ttagatattt tgatatagac taatgcttaa attaagcaac ttttctaaaa aagggagtat
    14941 gaaaatgatt agtactttga atgaaattat gaaatgtatc gaagacaacg atacaattat
    15001 tatacacaga catgttagac cagatcctga tgcatatggt tcacaacttg gtttaaaata
    15061 ctatattcag cagaaatttc cgcaaaagca agtatttgca gtgggtgaag cggaatcatc
    15121 attaagtttt attggagagt tagataacat tgatgataaa acatatcaag atgcgcttgt
    15181 aattgtatgt gatactgcca atgctccacg aattgacgat gaacgttata gtacaggtcg
    15241 taaacttatt aaaatagatc atcatcctgc agttgatcag tatggtgata ttaatttagt
    15301 taatacgaac gcgtcatcta caagtgaaat catttacgat ttaatctcac attttaatga
    15361 tgaagcaatt gttaataaag atatcgcgag cgttttatat cttggtatcg tcggtgatac
    15421 tgggcgattc ctttttaaca atacctcaga acatactatg gaaattgctg gaaagttaat
    15481 tgggcatgat attgatcata atgcgctttt aaataaaatg atggagaagg acccaaaaat
    15541 gttgccgttt caaggttatg ttttacaaca tttcgaactt atggatgatg gattctgcca
    15601 agttaaaata actgaagatg tattggagca attcggtatt cagccaaatg aagcatctca
    15661 gtttgttaat acaattgctg acatcaaagg cttgaaaata tgggtatttg cagtcgatga
    15721 aggtaatgaa atcagatgtc gattacgttc taaagggcaa ttgattatta atgatattgc
    15781 gcaagatttt ggtggcggtg gtcatccgaa tgcgtcagga gtttcagtgg acagctggga
    15841 tgaatttgag caacttgcta cagctttacg cacaaaactt aactaataga aaggggccat
    15901 tcaatcatgg tggcatattt aaatattcat acggcttatg atttgttaaa ttcaagctta
    15961 aaaatagaag atgccgtaag acttgctgtg tctgaaaatg ttgatgcact tgccataact
    16021 gacaccaatg tattgtatgg ttttcctaaa ttttatgatg catgtatagc aaataacatt
    16081 aaaccgattt ttggtatgac aatatatgtg acaaatggat taaatacagt cgaaacagtt
    16141 gttctagcta aaaataatga tggattaaaa gatttgtatc aactatcatc ggaaataaaa
    16201 atgaatgcat tagaacatgt gtcgtttgaa ttattaaaac gattttctaa caatatgatt
    16261 atcattttta aaaaagtcgg tgatcaacat cgtgatattg tacaagtgtt tgaaacccat
    16321 aatgacacat atatggacca ccttagtatt tcgattcaag gtagaaaaca tgtttggatt
    16381 caaaatgttt gttaccaaac acgtcaagat gccgatacga tttctgcatt agcagctatt
    16441 agagacaata caaaattaga cttaattcat gatcaagaag attttggtgc acatttttta
    16501 actgaaaagg aaattaatca attagatatt aaccaagaat atttaacgca ggttgatgtt
    16561 atagctcaaa agtgtgatgc agaattaaaa tatcatcaat ctctacttcc tcaatatgag
    16621 acacctaatg atgaatcagc taaaaaatat ttgtggcgtg tcttagttac acaattgaaa
    16681 aaattagaac ttaattatga cgtctattta gagcgattga aatatgagta taaagttatt
    16741 actaatatgg gttttgaaga ttatttctta atagtaagtg atttaatcca ttatgcgaaa
    16801 acgaatgatg tgatggtagg tcctggtcgt ggttcttcag ctggctcact ggtcagttat
    16861 ttattgggaa ttacaacgat tgatcctatt aaattcaatc tattatttga acgtttttta
    16921 aacccagaac gtgtaacaat gcctgatatt gatattgact ttgaagatac acgccgagaa
    16981 agggtcattc agtacgtcca agaaaaatat ggcgagctac atgtatctgg aattgtgact
    17041 ttcggtcatc tgcttgcaag agcagttgct agagatgttg gaagaattat ggggtttgat
    17101 gaagttacat taaatgaaat ttcaagttta atcccacata aattaggaat tacacttgat
    17161 gaagcatatc aaattgacga ttttaaaaag tttgtacatc gaaaccatcg acatgaacgc
    17221 tggttcagta tttgtaaaaa gttagaaggt ttaccaagac atacatctac acatgcggca
    17281 ggaattatta ttaatgacca tccattatat gaatatgccc ctttaacgaa aggggataca
    17341 ggattattaa cgcaatggac aatgactgaa gccgaacgta ttgggttatt aaaaatagat
    17401 tttctagggt tgagaaactt atcgattatt catcaaatct taacacaagt caaaaaagat
    17461 ttaggtatta atattgatat cgaaaagatt ccgtttgatg atcaaaaagt gtttgaattg
    17521 ttgtcgcaag gagatacgac tggcatattc caattagagt ctgacggtgt aagaagtgta
    17581 ttaaaaaaat taaagccgga acactttgaa gatattgttg ctgtaacttc tttgtataga
    17641 ccaggtccaa tggaagaaat tccaacttac attacaagaa gacatgatcc aagcaaagtt
    17701 caatatttac atccgcattt agaacctata ttaaaaaata cttacggtgt tattatttat
    17761 caagagcaaa ttatgcaaat agcgagcaca tttgcaaact tcagttatgg tgaagcggat
    17821 attttaagaa gagcaatgag taaaaaaaat agagctgttc ttgaaagtga gcgtcaacat
    17881 tttatagaag gtgcaaagca aaatggttat cacgaagaca ttagtaagca aatatttgat
    17941 ttgattctga aatttgctga ttatggtttt cctagagcac atgctgtcag ctattctaaa
    18001 attgcataca ttatgagctt tttaaaagtc cattatccaa attattttta cgcaaatatt
    18061 ttaagtaatg ttattggaag tgagaagaaa actgctcaaa tgatagaaga agcaaaaaaa
    18121 caaggtatca ctatattgcc accgaacatt aacgaaagtc attggtttta taaaccttcc
    18181 caagaaggca tttatttatc aattggtaca attaaaggtg ttggttatca aagtgtgaaa
    18241 gtgattgttg atgaacgtta tcagaacggc aaatttaaag atttctttga ttttgctaga
    18301 cgtataccga agagagtcaa aacgagaaag ttacttgaag cactgatttt agtgggagcg
    18361 tttgatgctt ttggtaaaac acgttcaacg ttgttgcaag ctattgatca agtgttggat
    18421 ggcgatttaa acattgaaca agatggtttt ttatttgata ttttaacgcc aaaacagatg
    18481 tatgaagata aagaagaatt gcctgatgca cttattagtc agtacgaaaa agaatattta
    18541 ggattttatg tttcgcaaca cccagtagat aaaaagtttg ttgccaaaca atatttaacg
    18601 atatttaaat tgagtaacgc gcagaattat aaacctatat tagtacagtt tgataaagtt
    18661 aaacaaattc gaactaaaaa tggtcaaaat atggcattcg tcacattaaa tgatggcatt
    18721 gaaactttag atggtgtgat tttccctaat cagtttaaaa agtacgaaga gttgttatca
    18781 cataatgact tgtttatagt tagcgggaaa tttgaccata gaaagcaaca acgtcaacta
    18841 attataaatg agattcagac attagccact tttgaagaac aaaaattagc atttgccaaa
    18901 caaattataa ttagaaataa atcacaaata gatatgtttg aagagatgat taaagctacg
    18961 aaagagaatg ctaatgatgt tgtgttatcc ttttatgatg aaacgattaa acaaatgact
    19021 actttaggct atattaatca aaaagatagt atgtttaata attttataca atcctttaac
    19081 cctagtgata tt
//
LOCUS       DAKTLH010000038        19039 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.038, whole genome shotgun sequence.
ACCESSION   DAKTLH010000038 DAKTLH010000000
VERSION     DAKTLH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19039
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.038"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(30..422)
                     /locus_tag="O0394_001127"
     CDS             complement(30..422)
                     /locus_tag="O0394_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499216.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKENYNKYIQGQKQRVIFENGHFV"
     gene            complement(472..651)
                     /locus_tag="O0394_001128"
     CDS             complement(472..651)
                     /locus_tag="O0394_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCY0499217.1"
                     /translation="MRRENILLRLIPFNDYDNKNFKMFIVIKNTKVIILKNKIFVFEQ
                     SIKKHNEMMYIELCD"
     gene            802..1800
                     /locus_tag="O0394_001129"
     CDS             802..1800
                     /locus_tag="O0394_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0499218.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            2032..2913
                     /gene="rbsU"
                     /locus_tag="O0394_001130"
     CDS             2032..2913
                     /gene="rbsU"
                     /locus_tag="O0394_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HCY0499219.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGLPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            2928..3332
                     /gene="rbsD"
                     /locus_tag="O0394_001131"
     CDS             2928..3332
                     /gene="rbsD"
                     /locus_tag="O0394_001131"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCY0499220.1"
                     /translation="MKKSAILNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            3360..4274
                     /gene="rbsK"
                     /locus_tag="O0394_001132"
     CDS             3360..4274
                     /gene="rbsK"
                     /locus_tag="O0394_001132"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCY0499221.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKVAHIDTSYIIKTAEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSIGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(4372..4506)
                     /locus_tag="O0394_001133"
     CDS             complement(4372..4506)
                     /locus_tag="O0394_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499222.1"
                     /translation="MIIIIINIAYLYAIIWKLKRLQKIVTSFITKHESTSIGLVENYF
                     "
     gene            4525..5286
                     /locus_tag="O0394_001134"
     CDS             4525..5286
                     /locus_tag="O0394_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0499223.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYNQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKEH"
     gene            complement(5720..7156)
                     /locus_tag="O0394_001135"
     CDS             complement(5720..7156)
                     /locus_tag="O0394_001135"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCY0499224.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(7172..7963)
                     /locus_tag="O0394_001136"
     CDS             complement(7172..7963)
                     /locus_tag="O0394_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCY0499225.1"
                     /translation="MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDTQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTS
                     YTPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     ISMIVPTKHAVGIQSEDGVDIVIHIGLNTVDLEGKGFKCFVKQNDHVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            8112..8816
                     /locus_tag="O0394_001137"
     CDS             8112..8816
                     /locus_tag="O0394_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0499226.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEI
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDQLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(8924..9625)
                     /gene="lrgB"
                     /locus_tag="O0394_001138"
     CDS             complement(8924..9625)
                     /gene="lrgB"
                     /locus_tag="O0394_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HCY0499227.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(9618..10061)
                     /gene="lrgA"
                     /locus_tag="O0394_001139"
     CDS             complement(9618..10061)
                     /gene="lrgA"
                     /locus_tag="O0394_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HCY0499228.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(10174..10914)
                     /locus_tag="O0394_001140"
     CDS             complement(10174..10914)
                     /locus_tag="O0394_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HCY0499229.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNNASAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPTYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(10917..12671)
                     /locus_tag="O0394_001141"
     CDS             complement(10917..12671)
                     /locus_tag="O0394_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0499230.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            complement(12918..13592)
                     /gene="scdA"
                     /locus_tag="O0394_001142"
     CDS             complement(12918..13592)
                     /gene="scdA"
                     /locus_tag="O0394_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HCY0499231.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAAALEK
                     KNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQSAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGELLVKMSELTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            complement(13732..15456)
                     /gene="tarS"
                     /locus_tag="O0394_001143"
     CDS             complement(13732..15456)
                     /gene="tarS"
                     /locus_tag="O0394_001143"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCY0499232.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKNQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFKGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISLKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMVMGMLSQTSENEVQILLSKDIKKRDFKNIVKLNTA
                     HITYPLNK"
     gene            complement(15489..17177)
                     /locus_tag="O0394_001144"
     CDS             complement(15489..17177)
                     /locus_tag="O0394_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0499233.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            complement(17199..18224)
                     /locus_tag="O0394_001145"
     CDS             complement(17199..18224)
                     /locus_tag="O0394_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCY0499234.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            complement(18217..18933)
                     /locus_tag="O0394_001146"
     CDS             complement(18217..18933)
                     /locus_tag="O0394_001146"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HCY0499235.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
BASE COUNT         6118 a         3553 c         2843 g         6525 t
ORIGIN      
        1 cctcttactc taattatatt cactatcaat tagacaaaat ggccattttc aaatatcacg
       61 cgttgtttct gaccttgaat atatttatta taattctctt tttgaaaatc agttaacatt
      121 aatttagatg taccgtattt taacactttt tgcattgttt ctattctcat ttttctaaat
      181 aaccatccat cttttaacac aatacgatta acagcatcat atgataattc tactgtttct
      241 ttaatttcaa atgtcttgaa tgaaataatc gtgcacatta aaaacgtatc accaaagtaa
      301 taaacatcta aatcatcacg tttatgttgt ccaactaaca aacgaccata ttcgaactct
      361 ttttctggat atttcaattc taaaaaacta ataatctctt cttcttttaa tttgaattgc
      421 atttaaaaac atcctctctt aagttttaac aagccttaat taaaaatttt ttcaatcaca
      481 tagttcaata tacatcattt cgttatgttt tttaatactt tgttcaaaaa caaatatttt
      541 attctttaaa ataatgactt ttgtattttt aatcacaata aacattttaa aattcttgtt
      601 atcataatca ttaaaaggta ttaaccttaa taatatattc tctcgtctca accttaatcg
      661 tatacttcag acgtctgttt gtagacaata aaagtcattc acgtcttcat atgtcatcaa
      721 atgtttatca tgatatgatg aatataataa tcgggtatat aactgtatga ttaattacac
      781 aataaagaga aggaatataa tatgaaaaaa gtgtcaatta aagatgttgc tagagaagct
      841 ggtgtatcag ttacaactgt gtcacatatt ttaaatcata atgatagtcg tttttccgca
      901 acaacgataa aaaacgtaca tgctgtttca gaacgtttag gctatgcccc taataaacat
      961 gcaaaacaat tgcgcggcag taaaattcaa actattggcg tcattttgcc tagcttaaca
     1021 aatccgtttt tctcagcact gatgcaaagt attcatgacc ataaaccatc tgatgttgat
     1081 ttatgctttt taacatctac agcaactgat ttgtatgaca atattaaaca tttaattgat
     1141 cgaggtattg acggattaat tatcgcacaa tacatatcat ccccggacgc cctaaataac
     1201 tatctaaaga aacatcatgt accttatgtc gtactggatc aaaatgacca tcaaggctat
     1261 acagattttg ttcggacaaa tgaatatcaa ggtggacaac ttgcagcaca acatttaata
     1321 gaactcggtc acaaccatat gataattgtt gcaccatatg acatgatggc gaatatgtcg
     1381 actcgtgtcg ctggatttgt cgatactttg cgcgcgaatc aattgccaga accacaaatc
     1441 gtccatactg aattatctaa gcgcggtggg ctaaccattg ttgatgacat catggttcaa
     1501 tctgccactg caatcttcgc tattaacgat gaactcgcta ttggcatttt acgaggacta
     1561 attgaacatg gcatcagtat cccgaaagat atctcattaa taggttatga cgacattgat
     1621 tatgcagcgt acgtctcgcc acctttaact actgtggcac aacctataac tgatattggc
     1681 aaaacatctt taaccttatt acttcaacga ttacagcact tagataaatc cattgatatg
     1741 attgaattac caacgacttt aaaaattcgt gcaacaactg gctatcatct ttcaaactaa
     1801 ctacgtatct tccgaaatat actcatcatt gttaggccct tagcgttgct ttaatgctga
     1861 gggtttttaa tcataattat tttactaaga aattaaaata ataatgtatg aatttttaaa
     1921 tatgatttaa acgttttcag tttttatgaa aacgcatgca ttttacaaat aaaaatggta
     1981 cgatggcact ggtaaaacgt tttactaaaa acaaatcatg aggtgtataa catgagcatt
     2041 gttgcattac ttatcgggtt aggcccctta attggctggg gcttcttccc aacagtcgct
     2101 tcaaagtttg gtggtaaacc tgtacatcaa attatcggtg ctactgtagg tacgttaatc
     2161 ttcgctatta tattagccgt agtcacatca agtggcttac ctactggaac caatttgcta
     2221 ttcgccttat tatcaggtgc aggatgggga ttcggacaaa tcattacatt taaagcgttc
     2281 gaattagtcg gctcatctcg tgccatgcca gtcacaacag cattccaatt attaggcgca
     2341 tctttatggg gtgtctttgc attaggaaat tggccaggca ttggtcataa aatcattgga
     2401 tttacagctt tagtcgttat tctaattgga gcgcgtatga cagtttggag tgaacgcaaa
     2461 gaagcaagta acgccaaaaa tttacgtcgt gcagtggtac ttctgttaat tggtgaattt
     2521 ggatactggt tatattcagc tgcaccgcaa gcaacttcta ttgatggcct aactgccttt
     2581 ttacctcaag caatgggtat ggtaattgtt gcagtcattt atggctttat gaatatgaaa
     2641 gcagagaatc cattccgtaa taaaattacg tggttacaaa ttatttcagg tttcttcttt
     2701 gcatttggtg ctttaacata tcttatttca gcacaaccta atatgaatgg tttagcaact
     2761 ggatttattc tttctcaaac atccgttgtg cttgctacat taactggtat ttatttctta
     2821 aaacaacata aaacgtcaaa agaaatggtt attacaatca tcggcttagt actcatttta
     2881 gtagccgctt ctgttacagt atttataaaa taaggagtgt agatgtcatg aaaaaatcag
     2941 ctattttaaa tgaacatatt tcaaaagcaa tcgcgacaat tggtcatttt gatttattaa
     3001 cgattaatga cgctggcatg ccaattccaa atgatcatcg tcgtatcgac ctagctgtaa
     3061 ctaaaaactt accacgcttt attgatgtct tagctacagt gttagaagaa atggaaatcc
     3121 aaaaaatata cttagcagaa gaaataaaag aacataaccc tacacaattg caacaaatta
     3181 aacaattgat ttcatcggaa atcgaaatca ttttcattcc tcacgaagaa atgaaaagta
     3241 acttagctca cccattaaat aaaggtaata ttcgtactgg tgaaacaacg ccctactcta
     3301 atattgcatt agaatcgaat gttacttttt aaaagttata acttgaaagg agcgtacaca
     3361 tgaccaacaa agttgttatt ttaggttcaa cgaatgtcga tcaattttta acagttgaaa
     3421 gatatgcaca accaggcgaa acattacatg ttgaagaagc acaaaaagca ttcggcggag
     3481 gtaaaggtgc caaccaggct attgccactg cacgcatgca agcagacaca acatttatta
     3541 ctaaaattgg cactgatggc gttgctgatt tcatcttaga agattttaaa gtagctcata
     3601 ttgatacatc atatattatc aaaacagctg aagcaaaaac gggccaagcc tttatcactg
     3661 tgaatgcaga aggacaaaac accatctatg tttatggtgg tgcgaatatg acgatgacac
     3721 ctgaagatgt tattaacgca aaagacgcta taatcaatgc agactttgtc gttgcacaat
     3781 tagaagtacc catcccggct attatatctg catttgaaat tgccaaggca catggtgtga
     3841 cgacagtatt aaatcctgca ccagcgaaag cattacctaa tgaattatta tcattaatcg
     3901 atattattgt gccaaacgaa acagaagccg aattgttatc tgggattaaa gtaactaatg
     3961 aacaatctat gaaagacaat gccaattact ttttatctat aggcattaag actgttttga
     4021 ttacgctagg taagcaaggt acatattttg ctactaaaaa tcaaagccaa cacatcgaag
     4081 cttataaagt aaatgcgatt gatacaactg ctgcaggcga cacatttatt ggtgcatttg
     4141 tcagtcgctt aaacaagtcg caagataact tagcagatgc tattgatttt ggtaataaag
     4201 cgagctcact cactgtacaa aaacacggcg cgcaagcatc tattcctcta ctagaagaag
     4261 taaatcaagt ttaaatgaat caaacacagc tatgatatga aggtttagca tataacatgc
     4321 aacattcgta tatcatggct gtgctttttt atctttataa aacatcatct attagaaata
     4381 attttccact aaacctatgc ttgttgactc atgtttagtt ataaatgaag tgacaatttt
     4441 ttgtaatctt tttaacttcc aaattatcgc atataaatat gctatattaa tgataataat
     4501 tatcaattaa aaggaggtta tgctatgtct aaagaagctg gtcatacatt tttagctaaa
     4561 ttaggaaaaa ctcgtctacg ccccggtggt aaagaagcaa cagattggtt aatacaacaa
     4621 ggggcatttt cacaagataa acaagtgtta gaagtggcat gtaatatgtg cacaacatct
     4681 atttatctag ctcatacata tggctgtcac attcaaggcg ttgatataaa taagaaagca
     4741 ttagaaaaag cacaggaaaa catttcagca gcaggtcttg aatcatatat tcaagttcaa
     4801 caagcgaatg ctgttaaatt gccctttgat gacaatcaat tcgatatcgt tttaaatgaa
     4861 gcaatgttaa caatgttacc catcgccata aaggaaaaag cattacgcga gtactaccga
     4921 gtcttaaagc ctgggggtat cttgttaaca catgatattg tcatcgttaa tgaatcacat
     4981 gccacacatg ttgttaaatc attatctgca gcaattaatg tcaatgtctc accgcagacg
     5041 aaacttggct ggttagattt atataatcaa gctggtttta atcatgtgca ttatcatact
     5101 ggtccaatga gtttaatgac accaaaaggt ttaatttatg acgaaggtat tgttggaact
     5161 ataaagatta tcaacaatgc tttgaaaaaa gaaaatcgac caatgttttg taaaatgttt
     5221 aaaacgatga ctaaattgcg taaagatatg aattatatta cttttgtcgc taaaaaagag
     5281 cactaaatat aatgccacta actgtacttt gtatctatgt ttgactatca ctttaatttc
     5341 tttgtgacac taatcatcta cttaacaata tcgttatcgt tgattagtaa gtcatcaatt
     5401 ttggttaaag actttcataa acactcaaac attaacacta tacatagtta gtggcattat
     5461 ttttttctta aaattttaac atcacgggat tggaacagaa atgatatttt cgcaaaattt
     5521 atttctgtcc ttccccaact tgcattacct gtagaatttc ttttcgaatt tctctgtgtt
     5581 ggggccccgc caacttgcat tacctgtaga atttcttttc gaaattcttt gtgttggggg
     5641 cccgccatta taacactgta ggtcctacta tattaattta tgtctcgggc tctaacccta
     5701 aaatacttat aatatacctt tataatttat ctccatttga tgctatcact tgttgatacc
     5761 aatagaacga atcttttttc aagcgttcca tcgtgccatg accatcatta tctcgatcaa
     5821 catatattaa accatagcgt ttcttcattt caccggttgt aaatgaaaca atatcaatga
     5881 ttccccacgg tgtataaccg attaaatcta caccatcttg atcaactgct tctatcgctg
     5941 ctgtaatatg tgcttttaaa tattcaatgc gataatcatc atgaatatgt ccatctacca
     6001 cttcatcaac tgcaccaaaa ccattttcca caataaaaag tggtaactga taacgatcat
     6061 ataacacatt caatgtatat cttaagccat ctggatcaat cgcccaaccc caatcactcg
     6121 ttgcgatatg cggattctcc acagaatgat tcaaaccacc gttgacgatg ttgttttcaa
     6181 ctgtagtatc aacatcatgt tttacagccg tagacatgta ataactaaag ccaatataat
     6241 caaccgtgcc ctgctgtaaa atactgtcgt ccccttcttg ccatccaaca tcatatcctt
     6301 ttcgtgccaa cattttttta gcatagcttg gataataacc tctcacttgg acatccggga
     6361 aaaagaaacg taagcgattc gcaatttgtg cttccatcat atctttcgga tgacacgaat
     6421 atggataaat gggtacatgt gaaatcattg ttccaatctt aaacttcgga ttaatctctt
     6481 taccaagacg aactgctaaa gcactggcta aaagttcatg atgtgctact tgatacaaga
     6541 cttcttcagg attatcattt tctgtcaatg ctaccccaga attcgtccat aaaaagatag
     6601 gatttgatgt gtccatctga ttattaattt cattaaacgt catccaatat gtaactttat
     6661 ctttatatct ttcaaataca acacgcgcaa aatgcacaaa ataatcgaca acttctctat
     6721 ttctaaatcc accataatgt ttcgctaaat gaagtggcat ctcaaaatgt gataacgtca
     6781 caacaggttc aataccttgt gcaattaatt catcaaagat acgatcataa aaggcgagtc
     6841 cttcttcatt tggcacatct tcatccccat tcggaaagat acgtgtccac gcaatcgacg
     6901 tacgtaaaca tttcaatccc atttctttaa acaaggcaat atcttcctta taacgatgat
     6961 aaaaatcaat accttcatga tttggataat agtgattggg atctatagat tctgtaatct
     7021 gacgtgcttt gccatgtgca ccactcgtca taacatcaat tacacttaac cctttaccac
     7081 ctttatcata tccaccttca aattgatttg cggcaagagc gccaccccac atgaaatttt
     7141 gcggtaattt tgtcattatt acaacacact cctagttttt aaatatttta aaaataacat
     7201 cttcagtcgt aatgatttca ttcattgtca gttctacttt tcctaaatcg gcagagttgc
     7261 taataacgac aataacgtca gcattgtagc cttgttgttg tatatattgc tggtcgaatt
     7321 gcaacaacgt ttgccctgct tcaacatgat cattttgctt tacaaagcac ttgaaccctt
     7381 taccttccaa gtcaactgta ttcaagccaa tatgaatgac tatgtccaca ccgtcttctg
     7441 attgaatacc aactgcatgc ttagttggta caatcataga tattaaacca ttgaacggtg
     7501 cgattacttt ggattcttca tgagctttga ttgctaagcc ttcacctacc attttctctc
     7561 taaaaattga atctcttact tctttaagta aaatacgacg accagcgatt ggtgctttca
     7621 ctaaaattgg tgttgttact tttacttgag gtgtataact tgtatcttga ctattaatat
     7681 tgtcagtatt ctcactctct tcgacattag cattcttaat taatcgctgt aattgctgat
     7741 acacttcatc gacatttcca tttaatttta tgactaactg cgtatcattt tctgctacag
     7801 atgtcacatc aactatttgt ttcacttcat ttgctgtaga aggaattgta tgttgcatat
     7861 gaattgtaat gcacttcgta tcatacgttg catcaacaat gttttcgaca ccacctacag
     7921 ctgtaagaat gtcttgggct tgttctttat atttattgct cataaatatt accccctcaa
     7981 cttgatagta gaatgaaaat gtatttactt acctccaatt ataattgtac cggttcaatt
     8041 tgtaaacgcc gatacaatta taatattttg tgctataata attacagaca aagtgaaacg
     8101 aggacagaat attgttaaag tatgaacata ttgctaagca acttaatgcg tttatacatc
     8161 aatctaattt caaacccggt gataaattgc caagcgtgac gcaattaaaa gaacgttatc
     8221 aagtaagtaa gagtactatc attaaagcat taggcttatt ggaacaagat ggtttgatct
     8281 atcaagcaca aggcagtggt atttatgtga gaaatattgc tgatgccaat cgtatcaacg
     8341 tctttaagac taatggtttc tctaaaagtt taggtgaaca ccgaatgaca agtaaggtac
     8401 ttgtttttaa ggagattgca acgccaccta aatctgtaca agatgagctc caattaaatg
     8461 cagatgatac cgtctactat ttagagcgat taagattcgt ggacgatgat gttttatgta
     8521 tcgaatattc ttattatcat aaagaaatcg tgaaatattt aaatgatgat attgctaagg
     8581 gctctatctt cgactattta gaatcaaaca tgaaacttcg tattggtttt tcagatattt
     8641 tctttaatgt agatcaactc acttcaagtg aagcttcatt actacaattg tctacaggtg
     8701 aaccatgttt acgttaccac cagacttttt atacaatgac tggcaaaccc tttgattcat
     8761 ctgacatcgt atttcattat cgtcatgcac agttttatat tcctagtaaa aagtaataaa
     8821 tacataaaaa cgtctatatc ccagttataa actggagtat agacgttttt ttacgataat
     8881 aacaatggct caaattgcta ttatcttgct taggtttttc gttttagaag aatattgcta
     8941 caaagacagg cacaactgct acaacaacta caccaactaa cactaaagct atacttgcca
     9001 ttgattcttc tacaggtcct aattctttgg ctggtgctac acctaatgtg tgaccacttg
     9061 ttccaagtgc taatcctcgg gcaatagggt tagtaattcg gaaaagcttt aagaatttat
     9121 tacctagggc ataaataatg acaccattta aaataactgc taatgatgtt aattctttta
     9181 taccaccgat accagctgat actggtaacg caatcgctgt agttgctgct tgaggtaaca
     9241 ttgataaaat aacatcattg gcaaattgtg ctaacttcgc aaaagttaaa ataattaata
     9301 acgctacaac tgtaccgata ccaatacctc cgatgatacg atgccaatgt ttaacaagca
     9361 cttcacgctt tttatataac ggaatcgcaa aacagattgt tgccggttct aagaagaagt
     9421 aaataatgtc tccacctatt ttgtaagtct tatacggaat gcctgttaaa tagaggaagg
     9481 ccacaccaaa taccatactg acaaatagcg gtgcgaataa gaagaaacga ttagtttttt
     9541 caaataatat ggtcgctaag aaaaatggta taacggataa cagtattccg aagtaaggtg
     9601 tgtttagtgc taagtggtta atcatgagct tgtgcctcct ctattttgat cttttttgtg
     9661 actttgtcac ctttagatct cgaagtaact ttcataataa tttgtgtgac atagccagta
     9721 caaataagta atagtattgt tgagacgatt attagtccaa tgattaaaaa tggtgcttgg
     9781 ctaatgacac ctaaagagtt aacaactgag ataccggctg gtacgaagag taagccaatg
     9841 ttatttgtta gtgtcgttcc tactttttcg acttcgccta acttaacagc accagtacat
     9901 aataatacaa ataataatac taaaccgatt actgatgcag gcataggaat tggcataaat
     9961 gattcaatta ttttcgatac aaagagtact aaagcaatta caatgacttg gtgaaaaaag
    10021 tgtgctggtt ttgatgcgtc tttttgttgt ttcacgacca ttgcctccta cgtttgattt
    10081 aactaaagta tagatggctc acttcgattt gcgtgatttt tagtccgaaa tacaaaatat
    10141 cataggtaaa atgcataaaa aaaaggatta ctgttaaagt aatcctatcg acgctttaaa
    10201 atctttcata aatgaacgtc caacttgcat cttgacacca tttgtcaata ttaccatata
    10261 agtatagtta aaccattgtt gcacttcttt aatgtgtttc gtgttaataa tatatgaacg
    10321 atgaatacgt ataaaataag tgggattcaa tcgtttttca taacgattca atggctctgt
    10381 tgtttcgtat ttatgattcg ttgtatgtat ggttgtaata ccattatgtg tgccaatccc
    10441 aataatattt tgttgcttta acatgtgaat tttatcgtca atttcaacag gtaagctttg
    10501 atcaaaattc gccgacatat cattcgcaat tgcacttgcg ttattatcat ctttggcttt
    10561 agtcgcacgc actttattga ctgcttgttc aatacgtttt tgaccaaacg gtttcaaaat
    10621 atagtctgtc gcatttaatt caaatgcctg tactgcgtat tggtcatgtg cagttgcaaa
    10681 aataatcgca ggtggctctt tcatcttttg aatcttagct cctaattcga tcccattttc
    10741 atccattaaa ttgacatcta aaaatataat gtcatattga ttgatcagta gtgcttccaa
    10801 tgtttctttt acattttctg cctcattaat ttcttcaaaa ccaccaattt catttaataa
    10861 atatgttaat tcattacgtg ctaatggctc atcatctatg attaatgctt tcatatttat
    10921 tcctcctctt gtctttcata aggaagtaca caccaaaaag tggtaccgct cgatgtcgat
    10981 tcaaattgta atgctgcgga ttttccaaat aatcctttta ggcgtaagtt taaattttct
    11041 aaagcactac cagttccaga ctctgattct acagatgttt ctcccaacaa atgcatttta
    11101 tctttagaaa taccctgacc attatcttgt acaataatac gtacatgtgt tgcagtttct
    11161 ttaatcactg acacgtcaat atcgttacct tgctttcgat tcgtaaacgc atgtttgatg
    11221 gcattttcaa ctaaaatttg aattaaaaat ggtggtacaa gcacatcgcg atatttgtct
    11281 tcaacattaa tattgatatt aaatcttcct ggataacgtg cttgttcgag tgataagtat
    11341 gcacgcactt gacttaactc tttatctaaa gtaatcgtat gttgcttaga gccttgtaaa
    11401 ttcgctctga aaaaataact caattctaat agtaactctc gtgccttttc gctatttatt
    11461 ctaactaaag ctgagatcgt gttaattgaa ttgaagaaaa aatgtggact cacttgtgcc
    11521 tgtaatgact taatctcagc atctttcaat aacttacttt gcgtttcggc ttcaccaagt
    11581 tcaatttggc tactaaaaat atttgccaat ccttctgcaa gttgacgttc cacaaaagtt
    11641 aaatcattag ggtttgtaaa atacatcttc aatgtaccga cgatagaacc atgcatctca
    11701 agtggtatca cgatagctgc tctaagcggg caattcggat gactacaacc aatctcttct
    11761 ttagtatgaa cttctttcaa ctttcctgat ttcaatacat ctttagacag acttgttaat
    11821 atttcatttg ttggtatgtg atgatcacta cctgcaccta catgcgataa gatttcattt
    11881 ttgcttgtaa ttgctacggc agatactttc attaaatttt taataatcat cgcaatttgc
    11941 tgtgccgatt ctctattcaa tccttcttta aaatacggca atgtctggtt catcaattgc
    12001 agtacatcat gtgtttgaac agccttcatt tgctcctctt gctttaatgt tgaaatgata
    12061 atagacataa aaatcgccgt accaacgcta ttaacaataa tcattggtag tgcaattaat
    12121 gatatgaggt caaccgcata tgctttgtcg tgggaaaatg ttaaaatgct caacatttga
    12181 atcatttcca taacaattcc aatcatggca cttttcgcaa tactcgggta acgcttgcgt
    12241 ctttgagctt gtaagccaaa ataaccagca attataccaa taaatataga tgagataaga
    12301 taaacttgtg catccgcccc acccatatac actctgaaaa tacctgaaat aacgccaaca
    12361 aatagaccta caaaagggcc accaactaat cctgcgacac ctatcgttaa tacacgtgtg
    12421 ttagctaaag atacatcatc atctaaacgg aagtacacac ttcctgacaa actatgttga
    12481 tgatcgatga cgataccagt taaattagac attaaggcaa acaaactgaa aataatacat
    12541 aattgccaac gtgctttcca tgtacgtcga cgattcatta agtttttaaa atatggaata
    12601 ttcatcaaca cataggccaa aataataatt aaacctacac gctcaagtaa taacattgtt
    12661 agcgatagca cgatagcacc tcagtgaatt ataattatgt ttattttatc ataatagtgc
    12721 atgtaaaatt catgaataaa ttattaaaaa aacacgctac tatttaatta tctataccat
    12781 taaattttca tatcaatttt tatgctaagt tgaccatttt tcttttcaaa taatgctgtt
    12841 tatgtcacta ttatgacttt tatacatagc gaaaaactgc tggacttatt tttgccaaca
    12901 gtttctaatc gcgttattta tgatactttt ttaaataata cgtgattctc taaatgtacg
    12961 tgttcatgtg ttaacacttc aagtgctttt aatctctgat aaacaagtcg ccaagtacca
    13021 cacgcttcta tcggaggttc ataactagat gttaattcgc tcatttttac taacaattct
    13081 cccgttgcaa tgtggtctga aactaaatca tctatcacag tattaatatc gtctactacc
    13141 tcaccttgct catatttaat gagttttgga aaatcgacat cgtcttcttt ttgcatatgc
    13201 tctaacatgc catttttaaa tgtatcgtat gtttctttta actcaactaa atatggatga
    13261 ttaggtccat gtactttcga taacttcgtc acataaggtg ttaaattttt aaattcttct
    13321 cttagaggtt catgatatgc tgattgaata tattgaataa gtgatgaaac atttaaaaat
    13381 ttaggattta acgaacctgg tgtattcgtt tgttcaacgt cgttgagacg ctgtaataat
    13441 tcgttcaaat ctacattttt cttttctaag gctgctgctt ctatacttac ttgtccgcca
    13501 caacaaaaat ctattcccac acttctaaaa atatccgctg ctttcggata atcagttact
    13561 acatctgcta ctatgtcatt tttatttatc attatagata acctcctcat ttccagtgta
    13621 tacaatttaa tgtatatgaa aatgaaaatc agggaaatgt cataatcatg tcataaacag
    13681 caaaataaag ccctcaaatc atgttggctc acaatgattt gagggcattt attatttatt
    13741 tagtggataa gtgatatgtg cagtgtttaa cttaacaata tttttgaagt ctcgcttttt
    13801 aatatctttg ctaagtaaga tttgcacttc gttttctgat gtttggctta acattcccat
    13861 caccatgtcg tcttttagta cgacactagc cataccttct tcaaatacat aaccacgatc
    13921 cagaccagtt aaaatgattt tgtttttacc ttttaacttc aatgagattg aatccgttaa
    13981 tgtaatagct gtaaagtata acgaaatgtt attaaagtct tttgtgaaat acggtgttaa
    14041 tctataaaaa tcattattca aatgaattaa tgcacttgtt tctgcagtat atgcatattt
    14101 attacgttga ttaccaacac gcacttcaaa tacttccgta cctatttgga cttctaagaa
    14161 aacatcccaa attttttctt taggtaaata cgctttaata tcttctaaca taatgttaaa
    14221 acgatattgt ttattcgcat tgacacttaa aggtacatac attgtgtgct tcttgtttcg
    14281 tgaatgtaat ttaagtctga catcataatg tccttcgcca atcataaatt catttaacgt
    14341 tagctctgta acaattttat gattatcaaa tttaaatgct gtcattttca cttttggctt
    14401 agcgatatca atgcctttaa agtatggttc gccttcattg aattgaatgt ttaatttacc
    14461 atctacaata tcaaaacggt agtattgacc ttgacggtaa ctttcctcca cagttctata
    14521 gttgtctata tctttcgctc tcgcgtagtg caacaatggt cgtaatttac tcatcaccaa
    14581 tgcatctaca cgttctggaa ctgcttgtat aaagtctcct agagcattaa tccattgtgg
    14641 ttgatcttct agtttaactt taagtgagaa gccattcgta cgagaaaaac taaaatgacg
    14701 atttaaaaat tctgctaaga tttgattttt atgggcttct tctaaatctg catttaatat
    14761 ttctacagca atcaatctca tgacttcata aaagtcttca ggtgaaacat acgcactact
    14821 catatgttca ccttcacgct ttgtagcata ataatacgct ttatcactta acacactgat
    14881 tcgatttgca tttaaatatg ctttcattgt aaataattga tcttctgcac tctttaattc
    14941 ttctggaaaa taaatgtcat tatcttttag taatgctgtt ctatagattt tagtcgggct
    15001 taaagtatag ataattcttg aatttaacaa agtaacttca ggtgctgttt ctttaaacat
    15061 agattgtggt acaccacgac cattaacacc tttcatttta atcaataaga catctgagtg
    15121 atgttcatca ataaatgctg ctgcatcctt taaagtctct ttgtttatat agtcatcgga
    15181 gtccacaaat aatacaaatt caccttctgc ttgttttaac gccacatttc taggtttacc
    15241 tggaccacca gaatttgttt cgagttgact tactttcaag ttcaatttat tgcgatactt
    15301 ttcaactatt tgtaacgttt gatctgttga acagtcatca actacaacca cttcaaattc
    15361 agttttcgga aaatcttgtt tcgcaaggct attaagtaat tctgttatat acttttctga
    15421 attgtatgtt ggaactatta ctgaaaattt catcattata cctctcccac tttgactact
    15481 atataaactt agctaccaaa taaatttctg actaaacgct cacttgatcg gccatcttga
    15541 tatttaaaat gtttatctaa gaatggaatg actttttctc cttcataatc ttcattgtcc
    15601 aaggcgtcca ttaatgcgtc aaatgattgc acaattttac ctggaacaaa tgattcatat
    15661 ggttcataaa aatcacgcgt cgtaatataa tcttctaaat caaatgcata gaaaatcatt
    15721 ggctttttaa atactgcata ttcatatatt aaagatgaat agtcactaat taataaatct
    15781 gttatgaaca gtatatcatt aacttctcta aagtcagaaa cgtcaacaaa atattgttta
    15841 tgtttgtctg caatattaag tctatttttc acaaatggat gcattttaaa taatacaacc
    15901 gcgttatttt tttcgcaata tcttgctaaa cgttcaaaat caattttgaa aaatgggtaa
    15961 tgtgctgtac catgaccact acctctaaat gttggtgcga aaagaatgac tttcttacct
    16021 ttaataattg gtaattcatc ttccatctct tgtttgatct gtgtcgcata agcttcatca
    16081 aatagtacat cagtacgtgg aacacctgta ggcactacat ttttctcttt aataccaaat
    16141 gcttcagcgt agaatggaat atcggtttca gatgatacat aagcttttgt atagctacga
    16201 tgatttaatg aatcaataaa tggtccaccc tttttaccag tacgactaaa gccaactgtt
    16261 ttaaaggcac caacggcatg ccatacttga ataacttctt gagaacgtct aaaacgcact
    16321 gtataaatca atgggtgaaa gtcatcaaca aagatgtagt ctgccttccc aagtaaatat
    16381 ggcaatctaa acttgtcgat gatgccacgt ctatctgtaa tattcgcttt aaaaacagtg
    16441 tgaatatcat actttttatc taaattttga cgtaacattt cgttatagat gtattcaaag
    16501 tttccagaca tcgttggtct agagtctgat gtgaacaaca ccgtattccc ttttttcaag
    16561 tggaaaaatt tcgtcgtatt aaatatcgct ttaaaaataa attgtcttgt attaaatgat
    16621 tgtttgcgga aatacttacg taattcttta tatttacgaa cgatataaat acttttaact
    16681 tccggagtcg ttacaacaac atcaaggaca aattcattaa catcgctaga aatttcaggt
    16741 gtaacagtat aaaccgtttt cttcgaaatg ccgccttttc taaattcttt taggtaagtc
    16801 tgcaataaga aattgatttt accattttgt gtttctaatt cgttgtattc ttcttcttgt
    16861 tctggcttta gattttgata tgcatcatta atcacatctg ggtttaactg tgcaatataa
    16921 tcaagttctt gctcattcac taataagtac ttatcttcag gtaagtaata accattatct
    16981 aagatagcta cattgaaacg acaaacgaat tgattcccat ctattttgac atcattcgcc
    17041 ttcattgtac gtgtctcagt taaatttctt aatacaaaat tactatcttc taaatctagg
    17101 ttttcactat gtccttcaac gaataactga acacgttccc aatagatttt atctatatat
    17161 atcttacttt taaccaacgt taattcatcc ttttctattt acataatcca ttttaatact
    17221 gttttacccc aagatgtaga caggtctgct tcaaaagctt ctgtaagatc attaattgtt
    17281 gcaatttcaa attcttgacc ttttaacaac gctaatttat ctacaatatc tgggtattga
    17341 atgtataagt ctacaacatc ttggaaatct tttgaaccac ttcgactact accaatcaac
    17401 gttagtcctt tttccaatac tagacgtgta ttaacttcta ctgggaactc acttacacct
    17461 aacagtgcaa tgcttccttc tggtgaaatg taatcgatca tttgatttat ggctgattga
    17521 ctaccgcgac cacccacaca ctcaaatgca tgatcaaatg ttaagccttc aggtatttta
    17581 ttaataaaga agacatcatc aacaaatgag aagtgactca atttataatc tgttttacca
    17641 aagacatata ttttagactc tggatataat ttacgtaata aaatggctgt aatgtaacct
    17701 aagttaccat caccccaaat accaaatgta tttttatttg aaatagattt cttttcaaaa
    17761 cgacgaatag catgcaaact tactgttaca agctctgtat atgaaataat acttaaatca
    17821 atatcatcag gtagtggtac agctctatca tgatttagca acacaaaatc ttgcataaac
    17881 ccatcatgtc cacttgatct gaagtagctc gattttaaat agttttcagc aatgacatcg
    17941 tctttttctg tcggcgtatt cggtaccata actacttttg tacctttatt aaacacacct
    18001 ttactgtcaa atacgacctc accaacacct tcatgaatta aagacattgg caatttctga
    18061 gataagacat tctcatcacg gctaccagta taatatcttt gatcggcagc acaaattgac
    18121 atatataaag gacgtacaat gacatagtca ctgtaaatat ctacgttgtt atacgtaact
    18181 tcaaattgtc taggtgcaac taattgatat acttgattaa tcatcggcaa taccacctcg
    18241 aataatagca ttcgctactt ttaaatcgta aggtgttgtt actttaatgt tatataactc
    18301 accttttaca agtcgaaccg gtttgtttgt ttctacaata atcttacaag catcagataa
    18361 aatactcttt tgctcatcac tcaactgtgc atagctttct tttaataaat taatattaaa
    18421 cgattgaggt gtttgacctt ggtacatttc attacgcact ggaattgcat caatcgtttg
    18481 attatcttta gatgtaacaa tcgtatctat agcatcaatc actgtatcta ctgcaccgta
    18541 ctctaaagca gcttgaatat tttctttaat aatacgatgc gttaaaaatg gtctaactgc
    18601 atcatgtgtc acaatgacat cgtcatcgtt aataccattt gttgattcaa tatgtttaac
    18661 gatattcata attgtatcgt tacgatcgct accaccttga atgacttcaa ttctttcatc
    18721 agaaattttg aattttctaa gtgtatcttt cgtatgcgtc atccattgtt gtggcgtcgc
    18781 gataataatt ttttcaaaat cattaattaa aataaatttt tctaatgtat ggattaaaat
    18841 cggtttgttg tctaaatcta aaaattgttt aggtaaaggt acgttaccca ttcttgagcc
    18901 tatacctcca gctagaatac cagcgtattt cataaaatac ttcctccatt caactatatc
    18961 tatatttaat tatttaaatt tcgttgcatt ttccaattga aaactcattt taaaatcaaa
    19021 actctaaatg tctgtgtat
//
LOCUS       DAKTLH010000039        18889 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.039, whole genome shotgun sequence.
ACCESSION   DAKTLH010000039 DAKTLH010000000
VERSION     DAKTLH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18889
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.039"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..282)
                     /locus_tag="O0394_001147"
     CDS             complement(<1..282)
                     /locus_tag="O0394_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="HCY0499236.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHH
                     STVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAID"
     gene            544..729
                     /locus_tag="O0394_001148"
     CDS             544..729
                     /locus_tag="O0394_001148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499237.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
     gene            complement(952..1530)
                     /locus_tag="O0394_001149"
     CDS             complement(952..1530)
                     /locus_tag="O0394_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0499238.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(1795..2175)
                     /gene="blaI"
                     /locus_tag="O0394_001150"
     CDS             complement(1795..2175)
                     /gene="blaI"
                     /locus_tag="O0394_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCY0499239.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(2165..3922)
                     /gene="blaR1"
                     /locus_tag="O0394_001151"
     CDS             complement(2165..3922)
                     /gene="blaR1"
                     /locus_tag="O0394_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCY0499240.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            4029..4874
                     /gene="blaZ"
                     /locus_tag="O0394_001152"
     CDS             4029..4874
                     /gene="blaZ"
                     /locus_tag="O0394_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCY0499241.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            complement(5400..5819)
                     /locus_tag="O0394_001153"
     CDS             complement(5400..5819)
                     /locus_tag="O0394_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0499242.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            5943..6914
                     /locus_tag="O0394_001154"
     CDS             5943..6914
                     /locus_tag="O0394_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0499243.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            complement(7086..7178)
                     /locus_tag="O0394_001155"
     CDS             complement(7086..7178)
                     /locus_tag="O0394_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCY0499244.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(7335..7682)
                     /locus_tag="O0394_001156"
     CDS             complement(7335..7682)
                     /locus_tag="O0394_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0499245.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK
                     LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD
                     EIREFFSKNHEGF"
     gene            complement(7701..8318)
                     /gene="cadD"
                     /locus_tag="O0394_001157"
     CDS             complement(7701..8318)
                     /gene="cadD"
                     /locus_tag="O0394_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="HCY0499246.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQY
                     LGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNE
                     KGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQK
                     LANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
     gene            complement(9434..9628)
                     /locus_tag="O0394_001158"
     CDS             complement(9434..9628)
                     /locus_tag="O0394_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499247.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYC
                     LLPLIVNLSIYGFYVFVKRK"
     gene            9738..10430
                     /locus_tag="O0394_001159"
     CDS             9738..10430
                     /locus_tag="O0394_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499248.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKS
                     KNIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEAN
                     DKNAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKN
                     YVEYKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAV
                     CGTAFAAGCSVG"
     gene            complement(10598..10747)
                     /locus_tag="O0394_001160"
     CDS             complement(10598..10747)
                     /locus_tag="O0394_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499249.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIV
                     FYEDK"
     gene            complement(11294..11610)
                     /locus_tag="O0394_001161"
                     /pseudo
     CDS             complement(11294..11610)
                     /locus_tag="O0394_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
     gene            complement(11603..12586)
                     /locus_tag="O0394_001162"
     CDS             complement(11603..12586)
                     /locus_tag="O0394_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCY0499250.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPE
                     VRKSNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQ
                     EFPEQLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLK
                     KAIHDRGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
     gene            13012..13755
                     /locus_tag="O0394_001163"
     CDS             13012..13755
                     /locus_tag="O0394_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCY0499251.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            complement(13982..14167)
                     /locus_tag="O0394_001164"
     CDS             complement(13982..14167)
                     /locus_tag="O0394_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0499252.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(14172..14510)
                     /locus_tag="O0394_001165"
     CDS             complement(14172..14510)
                     /locus_tag="O0394_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499253.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            complement(14756..15364)
                     /locus_tag="O0394_001166"
     CDS             complement(14756..15364)
                     /locus_tag="O0394_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0499254.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            complement(15850..16749)
                     /locus_tag="O0394_001167"
     CDS             complement(15850..16749)
                     /locus_tag="O0394_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HCY0499255.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            complement(16848..18314)
                     /gene="msr(A)"
                     /locus_tag="O0394_001168"
     CDS             complement(16848..18314)
                     /gene="msr(A)"
                     /locus_tag="O0394_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HCY0499256.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
     gene            18788..>18889
                     /locus_tag="O0394_001169"
     CDS             18788..>18889
                     /locus_tag="O0394_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCY0499257.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEI"
BASE COUNT         6525 a         2857 c         2575 g         6932 t
ORIGIN      
        1 atcaatggca cgatataaat agctccattt tccttttatt ttgatgtacg tctcatcaat
       61 acgccatttg taataagctt ttttatgctt tttcttccaa atttgatata aaattggggc
      121 atattcttga acccaacggt agaccgttga atgatgaacg tttacaccac gttcccttaa
      181 tatttcagat atatcacgat aactcaatgc atatcttaga tagtagccaa cagctacagt
      241 gataacatcc ttgttaaatt gtttatatct gaaatagttc atacagaaga ctcctttttg
      301 ttaaaattat actataaatt caactttgca acagaacctc atttactata tgggcatctc
      361 tttcattatt aatacaaagc tttgtatctc cgatattagg caaaggtgta aacgaattag
      421 gtgatactat aggattcaac attatattaa tcgtttcttt aataatgatt atcttcttat
      481 tttttataaa tcgtagtaaa aatatctcct atgattatat aaaagttcaa agaggtgaag
      541 ccaatgaata aattagaaaa atggtctttg gtatttatta tttttacatt tctctcttca
      601 ttagcacttt tatatttcaa atataatttt agttcggtta tgccagtcct aagtctttct
      661 attttaacta tactaatatt gttgacgagt attattactg tagtttcagt taatgatgaa
      721 gatacttaaa acgttcatta gtgaaacagg tattatatta aaaaagcgta cgaatttgta
      781 cgcttttttc ttgtgttatt attaaaatct cataacagga aaatattatg agattttaga
      841 cgctgtcaga acgtctcttt tacatcgagt gacattgaat caaagtcttt gtatgttaat
      901 gatatataaa attatttagt agattcatta tagtaagcta gattagttgc tctacttgtt
      961 tttattctct tttgcctcta atttattgag ataacgataa atagttgtac gtgaaacttg
     1021 ccactgctct gcaattgttt taattggcgt accattatca taaagtgtct taagtaagtt
     1081 taaatcttgt ttatttaatt tttcaggtct acctccatat cggcctctag cacgtgctgc
     1141 aattcgaccg gctgatgatc gctctaaaat taaattacgt tcaaactctg caaatgctgc
     1201 gaataaatga aataataatt gtcctgtaga acttgattta tccatcgtta tattttcttc
     1261 taaactatga aaactcacac ctcgttcgtt aagttcatta actaacgtga ttaaatcctc
     1321 catgttccgt cctagtctat ctaatctcca aacaacaatc gtatctcctg acctcgcaaa
     1381 ttcaattgct ttatctaaac caggtcgctt actttttgaa ccactcatat ggtcattaaa
     1441 tatcttttca caaccatatg tatttaatcg atcctcctgt aaattcaaat tttgtaatcc
     1501 agttgaaact cttgcataac ctattttcaa aatacagacc tcctatatac acctttagtg
     1561 taccataact tgattacgag gtcttttgtg aacatagatt ttttgttggg tttttgaaca
     1621 cataattcta tgtaatactt acttatttag acaaaaaaca agagtgtaca gaaaatgggt
     1681 tattttctgg acactctcat ctttaataat atattttaaa tgtcattaac aaaactaatt
     1741 taataagagt caagtatggt ttacatatgt attatgtttg aaacatacct ctaattactt
     1801 tttactaata tcatttaaaa tgtctcgcaa ttcttcaatt tctttgttat ttaattcttc
     1861 atttttcgca aaattcagca ctaaactttt catatcccct ccatacagtt tattaagaaa
     1921 ggttttagca gttttcattt taatatcgtc ttctttaata tttgatgagt aaaaataaat
     1981 attttctgat ttgtatcgtt ttataatctc ttttttatat agtcttgtga ttaatgttct
     2041 aatcgtttta tcactaactt ctttatattt ttgaatttca actacaattt cattagctga
     2101 tactgatttt ttatcccata ttatattcat aacatcccat tcagccatag atatttcaac
     2161 ttgcttattg gtcatttaaa acacccattc cttttaatat tttttcactt attaattcag
     2221 catttttccc agacgggttt ccatctgata aatgtgtagc aaaataatac ttatcatgat
     2281 ttgtaattac gtaacctaca aaccacccat tattatactt cccgtttact atacctgtac
     2341 ctgttttccc atacagttca tacttttcat ttttcttaat caataatgaa gaagataatt
     2401 gattctttgc ttttttacta aaatggttat tttgttccat catattttta aaaactatta
     2461 cttgttcaag attagatatt ttcaaactat cttccatcca atagctttta taacttccca
     2521 aattttcatt accataattt aattgcttga gttgagccgc agtatagttc tttggtattt
     2581 gattactaat acgttcgaag taccaattaa ctgaattttg cattgctgta tttaaatctt
     2641 gttccttatt ccaagactcg aaaagataat gtttatgatt ccatgacata cgtgaatttt
     2701 tatcacttat aatatgacgg tcaagtccaa acagtgctaa ataaattttg taagttgaat
     2761 caggagaata tctttttcta ctttcttttt cattataaat atagtatttg tctttcttca
     2821 tgctgtacat aacgaaagat ccactatttg aaccaaaatt tttactttca tctaaaattt
     2881 ggtaatcgct ttgtaagggc tttttataat tataatcagt taaggattgc cccattaaaa
     2941 actggctttg aattatcatg ataaaaaaag taaaaataca aataaaaatt agaattaatt
     3001 ttgactgctt tttcagatcg gcttctttta tattaattaa tctacttttg agtaatgatt
     3061 ttttaccatt aaatgaatgg cttaatatat ttttgttgga aaatggggtt tttaatacgg
     3121 agtccataat agattccgca taagttttaa attcattttt gttaatatta ttaaggacaa
     3181 atctatcggc ttctacttca ttgtcatgaa ttatctttct ttttacaata tgtactaatg
     3241 gattgtaact cataataata gagaagatat taaagataat taaatgtaaa gtatctctat
     3301 ttttagcatg agcatattca tgcagtatta tatattttag tcttttatca attacacttt
     3361 taaaatatga actaggaatc aaaataatat atttcccata ccaaaaagtt attggagatt
     3421 gaatagcctc tgcttttcga atcacaatat tttttttata ttgatggttg aaaagtatcg
     3481 tatctatttt atttttttca ttttcgttta gataaagtga ctgtttcttt aaatatttaa
     3541 gatataataa ggctttcaaa aatttaaaac ttaaaataat aactaaaact atccaaataa
     3601 ctgtgcagat attatcaatt gaatcccaat taaacttatg gatatctgtt gtgaactctt
     3661 gaataggttt ggtggtattt atgttatggt tcaaatcgtg tgacttactt tcaactgtgg
     3721 gcgcttgatt attcacatta ttaaatttta taaaagagaa tttaattggg ataaaaggaa
     3781 ttaatccagc aagcatagtt agataccaaa ctttataatt taaactataa ttaaaatagc
     3841 gttttaatat gtacctaaaa aatactaaca atagaaatat aaaacaaaag cttactacgc
     3901 tcattattag taatttagcc attttgacac cttctttcaa atatttataa taaacaattg
     3961 acatcaatat tacaattgta atattattga tttataaaaa ttacaactgt aatatcggag
     4021 ggtttatttt gaaaaagtta atatttttaa ttgtaattgc tttagtttta agtgcatgta
     4081 attcaaacag ttcacatgcc aaagagttaa atgatttaga aaaaaaatat aatgctcata
     4141 ttggtgttta tgctttaaat actaaaagtg gtaaggaagt aaaatttaat tcagataaga
     4201 gatttgccta tgcttcgact tcaaaagcga taaatagtgc tattttgtta gaacaagtac
     4261 cttataataa gttaaataaa aaagtacata ttaacaaaga tgatatagtt gcttattctc
     4321 ctattttaga aaaatatgta ggaaaagata tcactttaaa agaacttatt gaggcttcaa
     4381 tgacatatag tgataataca gcaaacaata aaattataaa agaaatcggt ggaatcaaaa
     4441 aagttaaaca acgtctaaaa gaactaggag ataaagtaac aaatccagtt agatatgaga
     4501 tagaattaaa ttactattca ccaaagagca aaaaagatac ttcaacgcct gctgctttcg
     4561 gcaagacttt aaataaactt atcgcaaatg gaaaattaag caaaaaaaat aaaaatttct
     4621 tacttgattt aatgtttaat aataaaaacg gagacacttt aattaaagat ggtgttccaa
     4681 aagactataa ggttgctgat aaaagtggtc aagcaataac atatgcttct agaaatgatg
     4741 ttgcttttgt ttatcctaag ggccaatctg aacctattgt tttagtcatt tttacgaata
     4801 aagacaataa aagtgataag ccaaatgata agttgataag tgaaaccgcc aagagtgtaa
     4861 tgaaggaatt ttaatattct aaatgcataa taaatactga taacatctta tagtttgtat
     4921 tatattttgt attatcgttg acatgtataa ttttgatatc aaaaactgat tttcccttta
     4981 ttattttcga gatttatttt cttaattctc tttaacaaac tagaaatatt gtatatacaa
     5041 aaaatcataa ataatagatg aatagtttaa ttataggtgt tcatcaatcg aaaaagcaac
     5101 gtatcttatt taaagtgcgt tgcttttttc tcatttataa ggttaaataa ttctcatata
     5161 tcaagcaaag tgacaatagc attttacaag tattttttat tgttcgatta gggtaaacct
     5221 aaataataca taaattatat tacaatatgt ttattataga ggctaaagta tcttagaaat
     5281 ttggatagta ggtttttcgg gaataaaagt atagttggct ttaatatata attaagctaa
     5341 gctatcattt atatttactc gattataatt cgcacttcaa cttttttata acgtaaaaat
     5401 taaaacgtag ttttatttat ctttagtaat atctttttaa acactacagt ttcttcatta
     5461 gttaaattag cgaagtaatg attattaagt tgataggctt tcttttctaa tgttggccat
     5521 aagtcttttg ctttatttgt actgtttatt agcacagttc ttctattttc attatcagtt
     5581 tgacgtgtta ttagatcctt tttggacatc ttatctaata ttccagatat tgttgaaggt
     5641 tctaaattta aagtattacc aatcgcttta ggtgtattca aattactatt aatcaagcaa
     5701 ttcaaaacac catattctgc tggagtaata ttatagggac ttaataaact tttaaaatgt
     5761 ttaaaaactc tattctgaga agtactcaac aaataattta tacaattatc taattccaca
     5821 actaaaacct ctcttaaatc attagtattg actcgcttat catatcatga taagttttgt
     5881 aggaaaatag ttttgcaaca aaaacatact tctacaaaac aattgtgaaa gaaggctaat
     5941 aaatgttcaa tgcggtagta gtgagaaaaa gtgcatcaaa tgttagttat aaagtagaaa
     6001 aagttgatga atctcattta tcgaagggtg atgtattagt aaaagtggta tactcttcaa
     6061 taaattataa ggatatgcta gctttacaat ataaaggcgg tgtaattaga gattatccta
     6121 tgattcctgg tatagatttt gcaggtattg ttgaatcaag ttcgaatgat aagtttaaag
     6181 aaggcgataa tgtattagtt actggttatg atgttggcgt gacacataca ggtggatttt
     6241 cagaatatgc acaagtgcct ggagagtgga ttgttcctat tccccaagaa atgtctatta
     6301 aagagtctat ggtatatggc acagccggtt ttacggcagc attatcaatt tttgaactag
     6361 aaaaatatgg aatgaatatc aaatcacaac ctgaaatttt agtgtctggg gctacaggtg
     6421 gtgtgggcag tgttgctata caaattttac ataaaattgg atatgaaaat atcactgcta
     6481 tcgtaagaaa agaaaagcaa atagaaatcg caaaaaaatt aggtgcaaaa caagtaataa
     6541 taattgaaga tgataataaa ccgcttaaaa aaggtatttt tgattatttc ttagatacag
     6601 tggggagtaa tgtaacatta tacggtttaa aacgtctaaa ttatcaaggt gttgctactg
     6661 tatgtggaaa tgtagcagga aattcactta acgctaatat attaccgttt attcttagag
     6721 gtattaaatt aattggcatt gattctgtat atgttgatca taatatccgt gaagatatat
     6781 ggagtaagtt agcaaatgaa tggaatatag gaaattcact tctatacaat gaaattggat
     6841 ttgaagaatt ctataaaacg atagatcagc ttttaaaagg acaacattta ggaagaacta
     6901 ttttaaaagt ataagtgtga gtattcatta atacatttac tattaattca atcattttgc
     6961 atttctctac taaccagtgt tataatttat ataacgaaaa tgattaacaa ttaatgcacc
     7021 aatccccaca ctattgcagt agtgtgggga ttttttcgat gctggcttat aaagtcgcct
     7081 attaattatt tgttgcgttt actaagccaa taagtataat acgcaacagc acagccactg
     7141 atgactggtg ctatgatgtg aacgaaaatg attagcaaag taatgcacct cctctcttcg
     7201 tcaattgacg cctgagagat aggcgactct attattatac catctgtgaa tatacaagca
     7261 tatctacttc cgataagtcg tcttatgtta tataactttg ttttttatag tatcaagaca
     7321 agaagaaact cgtttcaaaa accctcatga tttttagaga aaaactctct tatttcatcg
     7381 tctttaataa aataatatgc catctttcca tctttataaa aatcaagtac ctcattttta
     7441 tataaaagcc ttaaatgatg tgaagttgaa gcaacactca ttttcaatat caaagaaata
     7501 tcacaaacac acaactcatc ttctttaatc aaagataata tgattttcaa tttcttctca
     7561 tcacaaattt tttctaaaat gttaagtaat ttcttagatt tatcatcttc taaaaaactt
     7621 aaagcattgt taactttatc ctcatgtaca cagatcacat cacaagtatt ttcataactc
     7681 atataaaatc acaccctaaa ttaaaatata aatcctaaaa ttgtttgaat agtgtcattt
     7741 tcaataataa taaataaacc taaagctata taaataatag ccataatcca acgactaaat
     7801 ttctcaacaa tttctccaat tcctggaata ttagctaatt tttgtgcagt aaacaccaag
     7861 aagaaaatta aaattaaaaa gacaaacaga gtaagtaata aattagtaac acttaatgtc
     7921 acaaaatacg gaacaaataa accaatatta tcggcgccac aacttgctat cgtaacaatt
     7981 gcaaccgtac caactaattt agacaatcct ttttcattca attctttttt agctctcttt
     8041 tctccttcac aatcatcata aatagccact ttaattccaa gataaattgg tattaaaccc
     8101 aataaaccta atatccactt ttctggaaca taatttaata caaaagctaa aaacaaacta
     8161 actaatatta aaataataga ccctaaatat tgaccaacat aaatatctct atattctttt
     8221 ctagtctttg ctctagcaaa aaatattaat aaaattacta ataaatcaac tgctgtagca
     8281 atataaagaa cagcagcagc aacaaccgtt tgaatcataa tacacctcat tcaaaaatat
     8341 ttttgaatgt attataactt atatttttaa ataatcatag gtgctttatt ccaattgctt
     8401 tattgacgtt gagcctcgga acccttaaca atcccaaaac ttgtcgaatg gtcggcttaa
     8461 tagctcacgc tatgccgaca ttcgtctgca agtttagtta agggttcttc tcaacatcaa
     8521 taaatttttt cggcataaat gcgtgtctat gcgtagacat ggagacactt cagaattatg
     8581 gagtgattac atgagtttaa ctatttttct ttattcagtt ttaggaagtt tattagcaaa
     8641 ttatatctac gaaaaatctt caaaataaat aactttttcg gttctacttt gtacacttta
     8701 tctacctggt agacctaatt gttattgctt tacaggtcta aaattcgtcg ttataatcta
     8761 gttaaatccc gtaagggcgc acttatacgt cggataaatc gacatgtgca aaaacgtaat
     8821 cgaccttcgt cgattttttt atttcaaaat taaagaaacg gcttgagata ttccgagcag
     8881 ttataaaaat taatttgcgt tgaatattgc tactacattc ttgaaaaagt gatgtagatt
     8941 tatagcccat aacttttttg aattacatcg acgatacggc gtcgaaagaa aaataaatag
     9001 accgtatgaa agaaaaagct acctcacttt atctggggta gcttttattt tttatgccct
     9061 tattttgaat tttaagaggg cattttaaag atttaggggt gattcatata gttttatatc
     9121 taaaacttat atatgctttt aaaacgcaaa tatgagccaa ataaatatat gctattttca
     9181 aaaacaaaaa tttgagcaaa aacaataact atttttagac actgcccatt tacatgcaaa
     9241 ttaaaaatta gcataaaatt ctttaaaact tccccacgtt tttctaaatt cctgtagggc
     9301 gtttttatca aaaatcaaga caagaagaaa ctcgttcaca ctcgtttcaa aaaaacaaga
     9361 aatctaatct atacacctac cgttttccaa ccccctcaaa gcatacaaat taaaaacaaa
     9421 attattcata atcttacttt cttttaacaa aaacataaaa accataaata gacaaattaa
     9481 ctatcaaagg caataaacag tataaaaaaa ccacgaataa atcttcatca acatctaaaa
     9541 taaaaaagaa taaaacaatt aacaaaatac taattaacaa acctaaaagt ggcaaacttt
     9601 ttatatattt ttctaaattt tcattcataa aatagactcc tttcaaaaat atgtttataa
     9661 atatattaaa ataaatattg attttataaa gcgcttactt atacaattaa aatatcttat
     9721 tatcttggag gtatcaaatg aaaattataa aattattttc aataatttcc ttgttcctac
     9781 tggtatttag cgcgaatttt tctaatgctt acgcaaatga agaacaaaaa agttctttac
     9841 tagaaaatca aaaagaaaaa ttgaaaagca aaaacatcga agtatcacaa atgaccaaga
     9901 aagaaataag ttttctagaa caattgcaaa ataaaaatga agatatgcaa aaattcgaaa
     9961 agcaaatgaa aaaagaagga tttaaaaaaa taaagctaga agacaaaaat tctaaattaa
    10021 aatatctagc tacagaagca aatgataaaa atgcatatgg ctacatagct aataacgctt
    10081 ataaaaatga aaaaacaaaa gaaattgtag tttctgaaac aatatatgat aactacaaca
    10141 aaaaaatcac aaaattcatt gccgaaaaaa gaagtatgac tgaaaacaaa gagggaaaaa
    10201 tacttattaa caaaaattac gttgaatata aggctgaaaa agttgataaa aacagaccgg
    10261 taacatatgc aggattcaaa tggaacggca aagcattcgc ttgtagtatg gctggacttt
    10321 atgcatgtgc tcactactgt ggagtatggg caatcgtaaa tccaatagcc ggcggtgtat
    10381 gtgaagcagt ttgcggaact gcatttgcag ctggatgctc tgtaggttga ttttattcca
    10441 attgttttat tgacgttgag cctcggaacc cttaacaatc ccaaaacttg tcgaatggtc
    10501 ggcttaatag ctcacgctat gccgacattc gtctgcaagt ttagttaagg gttcttctca
    10561 acatcaataa attttttcgg cataaatacg atgttcctta tttatcctca taaaaaacaa
    10621 tagctgaaat atataaccaa ttactgtgtg tttgaatttc taatacatga atatcattct
    10681 ttaaaaacct attaacttgt ttatctaatt gacgatgagt aaaagtagcg aaagttttaa
    10741 tcttcatgat atctctcctt ttataaaaat agcaatcctt attacaatat taaacacttt
    10801 tattttggat tttaagaggc attttgaaga cttagaggca aatcatatag gttttgtact
    10861 taaaaatcta tacagtctct taaaacgcaa atatgagcca aataaatata tgctaatttc
    10921 acaaacaaaa atttcagcaa aatcagtgac taatttttta gatactgccc atttacatgc
    10981 aaatttaaaa attagcataa aatactttaa aacttcccca tgtttttcta aattcctgca
    11041 gggcttttct tatcaaaaac ttagaaattt taatttaact ctaaaatctc cgtaagagca
    11101 taagggaaat ttgctatcat tcaaaaatga tagatctggc aaggccagac attccaaggg
    11161 ttttaagtta ttttaaagtg atatcataca aagctgtagt gtcacacttt tgtgacacta
    11221 catacagcat ttgtcacaaa aataagacag cactttttta cagaaaaaca ataaaaaaag
    11281 acatcagtta tcactaatgt cttaaataat aattttgctt ttcgaaattc caaattgcgg
    11341 taattgaatg tgcttttttt cttcgtctgt cgttttatct tcatcacttg tattaatcaa
    11401 attaccatct tcggcatcgt ctaagtttaa aattttatgc ttttgtttaa gcaatccacc
    11461 ataacttaac atacgttttc gatacaaacc tttttctaaa tcacttacaa tttcttgatt
    11521 tttttcgtcg tcatcagtta aaaagtcaga tgatttaacg gaatattttg atgtctcttc
    11581 gagtgttata aaatcatttg aattattgat taaagatatt atattgtttc atgtttttac
    11641 ggcgcatata aagggcatgt gtttcttcaa gttcagttag aattgtttgc attaaataag
    11701 cttgcattga cttgattgtt tccttttggc ctttaactcc cctatcatgt atagcttttt
    11761 taagtctttt taaagaagtt aatatttcat cctcaatgtc ctctaacatg taatgcatat
    11821 cttcatgcct tgaattaaat gatttttttg ctttaagaat aacagccttt atgatttcta
    11881 aatctctata atcatatttt aataaataat tagttagttg ttctgggaat tcttgcaata
    11941 gcatttcttt atcatcaaaa ttatgtgtaa attgatttga atgatttgaa tgattactaa
    12001 tttcattttt gtactttata tcattcaagt cattcatatc attattctca gtatcattaa
    12061 agtcaatgtc attaaagtca gtatcattag tgttcgattt tcgaacttct ggacgttcga
    12121 ttttcgaact tctggacgtt cgattttcga acttctggac gttcgatttt cgaacttcct
    12181 tctcgccgta aggttcaaca tcgttttctt ctttttctat tttgtaaata tcacgttctg
    12241 tgacaatagg tttcattaaa tatagaatat ttggtttatt taaaccacgt cgtttttgaa
    12301 tgagtagacc atcgttttct aacccttttt ttatttttgt taacttttct tttttacaat
    12361 ttaagatttc cattagcttt tcatttgaat aaacaaagta aatgttacca tcttcatcaa
    12421 cccagctatt tttaatagat aaatttaagc gatcacgtaa gatagcatag gctattttca
    12481 tatcattagt tagattttta taattttcac tggtaaagaa tactttaggg atttgataaa
    12541 atctttcttt gtaattttct tgcactgtaa aatattgatt ttgcatgaca aaaaacctct
    12601 ttccatattt tagtttggta agaggtaata aaacgttttt atttagcttt acatttattt
    12661 aatgatttag gtgcttgcac taagatatta ttcattatat aatacaagta tcgaaaatta
    12721 tataaataaa cagtttctaa acgttttatt accaaacccc gactattacc agtagttggg
    12781 gtttttgtta tattaagttt attatagcac tttaaataaa cttataaaag ggaaaatata
    12841 gctttatcaa gggtttataa acccttgata aatatcataa acctttcata aacccttgat
    12901 aaaaaggttg atggatgttt atttttattt attaatcact ttattattaa gcattataag
    12961 cttataatat tttatcaagg gtttataaac attttataaa gggttgatga tatgaaaagt
    13021 gtgaaaaagt tgagtgaaga attaggggtt agtaagcaaa cgatttttaa taatattaag
    13081 cgattgaata tagagacaat aaaacaggaa aacacttcgt ttattaaaga agataccgat
    13141 atagaaaaaa tcattcaaag agtaaatgaa aataaaaaga aatatggttt tgaaagtact
    13201 actgaagaca aacaaaaaaa agggtcggat aatattaatt ttgaatcgaa aagcgatgct
    13261 caaatagtag aaatccttaa aaatcagatt gatactttaa ataatcaaat tgaaaaacaa
    13321 gaaagtcgtc atgaaacaac aattgaattt tacagaaaag agttgcaaga aagatcaaaa
    13381 ttgcttgaaa atcagcaagt tctggcatta gagagtaata aaaagattca aaaattagaa
    13441 aatcaattag aaaaagaaaa acaccttaat tatccctttg atacatctac taacactaga
    13501 caaaacgttg atgcacaaga aaaaacatat actactaatc ctgtaaatat taacagagat
    13561 caggaagaaa ctaaagaaac agagattcaa tataaggata tatctggaag tcaaagcgac
    13621 gaaagtacac aaggtgaaga agcaaaaaaa gaagacgttc cagctaatct aaatgacaat
    13681 gatagtgata tagaagaaaa gagtgaagaa acggaaccta aaaaggggtt ttggagtcgt
    13741 ttgtttggta gctaaaataa ttgattaggc actaaaagtt ataaaatttt agtgtctttt
    13801 atatagtgag aacttctggt aatcatcgat tatgtaaact aaagtatttc aaataattaa
    13861 ctgtattaat actctcatca tgattgcgtt ataaacgttc gttattgaga caaaatttaa
    13921 tagtactact acacaaaaag agagtgcgaa ttcgcactct ctttttgtga taaatgaatg
    13981 gttaagaaaa taattcatcc acagttgtat caaacaatct tgccaactta aatgctaatg
    14041 tcaatgtagg atcatattta ttgttttcta ttgcattaat agtttgccta gacactttta
    14101 attctttact taaagtttct tgagaaattc ctttttgttt tctatattct ctaattctat
    14161 ttttcattaa tttaaccttt agatgtatat tttttatttg tagtatagta attaaacagc
    14221 ataagaaata gtgctacaat tataaagaaa atttctggat attgagaaaa ccaagatcct
    14281 tcaatacttg gtgacaaacc tgtaaattta agcactagtg taaataacat acaatataca
    14341 atccaaataa aacttttagc cagtatttca ataaacattt tacgttgtct ttcatcgtat
    14401 cgatcttgtt gtcctttttt atctaatgtt tctgaaggta aatatttgtt agttatagcc
    14461 gtccataaac aagaaaaaat taaaataata ataaaaggaa ttaaattcat tataacacct
    14521 cataaatgta aaaagtattt gacatttatg agtgtaaata tttttgatac aaatgtcaaa
    14581 tactttttac attataatat attttaaaac accttattag taaaacaatt actatataga
    14641 aagctgcgaa ctcgtactct tttttcttgt ataattatta aaatctcaat tcaataatat
    14701 gttatggaat ttgagacact gtgagagacg ctcatttgca ttgacaatca atttatcaag
    14761 ataatccctt atcatgttta attctataaa ctgtttgtct tgtgatatta acttctttcg
    14821 caatcttact aatcgctttg ccttcttcta acatttccac cacgcgatga taaataacac
    14881 gtttctgtgg gtctttagca tttggtgaat aaagtaaagg gcgcccttta tatacgcctt
    14941 tttccttcgc aacttgaatc ccttgcgctt gtcgacgttt actttcattt ctctcttgtt
    15001 ctgaaaccat tgctaatatt tgaatgatta aatctttcat aaacttatcc agtaatggat
    15061 tgccaattac ttcattcatc ataggtaagc tggtaatcat taactgaacg tctttttctt
    15121 ttaaataatt cacagtttca ataatttcat catagttacg tcctaaacga tcaatagatt
    15181 ccacaacgaa tcgatctccc attctgacaa aattgagtgc ttcttgaaat acaggtctat
    15241 tttccactga ctttcccgac tgtttctctg taaagatttt ctccacacca aacgtcttta
    15301 aattatccaa ttgtctttct aaattttgat ctatcgatga tactctcgca taacctataa
    15361 tcattcatct tcactccata ataaatatac accctaatca tacgttttaa ttgttataat
    15421 ttcaacaatt ctttttgtat ggttagggtg tactcaaagt gtacatatct ataattaaaa
    15481 cttactaaaa ataaatatag attttttctt tgtaacagaa ccgttcattt gaaggtcatc
    15541 tctatcataa atatttaaat taactaaaaa ttttaatcat ctgtttaata cttatgaaat
    15601 gtatttacgg taggtattaa aattgatgaa atttatataa gtgctttcaa tgaaaatgac
    15661 cgtttattta tcctcctgat ttaaggagtt agaaatttcg aaaacgattc tttccatttg
    15721 ttgcatttgt tccatgtcca gatatttaat acattataga aaaccaaaga ccatcactaa
    15781 ttaaacgcag ttttaaaatt tcatcaggat tgccttcgtc tttaaaatgg gcctctttta
    15841 atataagttc tactctttca tacctaactc tttcaatgcc atcgtttcat acgccgattc
    15901 tcctgattcc atagcaaact cagcgataaa cattgggaat actgcattga gttctattat
    15961 atgctctttc attcgtggcc atatttcacc tccagcttta ccgtatgctg ttataagttg
    16021 ctctaatcct tcgtcgtcga atacacgatg gtgtcctata aagtccattg atgggtcgga
    16081 gtgggttgct tcagtccagt ctatgagacc tgtgacgttt gcttggttat ctaccattat
    16141 atgtcctgga tgtaagtccc catgtatcat ggtcgcatgt cgaggccata gttcgtcgtt
    16201 ttctaaccat tgtttccatc tgttccataa ttcatctgat acgccataag tttctttaac
    16261 tttattcatt cttctttgaa agtcattttt tatttcttgt atggttttga tatttatatg
    16321 ctgaacgtta atgttttctt ctggtatgtt gtgtaaatct acgagtgttt cagctaatgt
    16381 gttaataaag ttttctggta atggtttgtg ttcaatttcc catacataat tttgtatttc
    16441 tggatctatt gtggctgcgg gtttacctgt aagttttggg taagcaataa ggtcttttgc
    16501 atgtactttc cacttcggta tttcaaatga aacattcttc tgtaagaagt ctaccgtttg
    16561 tttttcgggt tttgttcgtt tataaacgtc aggtcttctt ggtagtctca aaacccattc
    16621 tactccatgt ttatcttttc caaatgcaac ttgaaaatca agtcccgatt cattcaatga
    16681 gattgtttga ggttggatgt gtaattgata tttttctgca catttaataa tttcattatg
    16741 tcgagtcatt ttattggatc cttatttcta ttatattttt cttgtgaacg tattttttat
    16801 attgatataa atgaacctaa tattcaatat aaattagtgt atagttttta agttatatca
    16861 tgaagagatt gtcctgttaa ttcccatttt ttatctgata catgtttaac aaaccttgta
    16921 tcatgtgatg taaacaaaat gattccagga tatttattca taaacatttc taatgcttct
    16981 aatgttttaa tatctaaaaa attagttggt tcatccaaaa ttaacatatt cgctttcgtt
    17041 gaaaataata ctgctaacga taatttcgtt ctttccccac cactcaaaac attacaagaa
    17101 cgatcaagtg cttcatttaa acccaagtta tttaaaatag ctctactgaa tgattctgat
    17161 gaatccgttt catccattaa atattgcaat aatgaaacgt cacgcatgtc ttcataagca
    17221 agttgacgat agtatgccat ttgcacttta ggagaacaat caattccctc tatttggtgg
    17281 taaatagctt caagtaaagt tgtcttacct acaccatttg cacctacgag cgctatattt
    17341 ttgccatatg gtatttggaa tcgtacttgt gttagcagtt tttgacttcc tttaaccaat
    17401 gttagatttt gtgcaatgat tggataatta ttatggatat cataaatttt attttgtggg
    17461 aaattgaatt catgataact ttgtggtttt tcaacttctt ccaaatgttc cattcttttt
    17521 tcaatatgct tagcttgttt ttgagcagcc ttctcaaccg tgcctttttg ttttgatgca
    17581 cttaaacgat ctggtgctat acttttgttt ttttgttttg atgatgcttg tgccatttgt
    17641 tgcgcttgat ttcgtttagc tttactggct tgggacaatc tttgtttttc acttatatac
    17701 tgttcatatt gacgttgttg ttctaactgt tcttgttcat attgattttg atactgtgtg
    17761 taattacctt taaacactct tattgtgcca tcttcttgta tatcccaaat tgtgtcagca
    17821 atttggtcta taagtgctct atcatgactt actataatta aagtaccata gtaatatttt
    17881 aaaatatttt tcagataatc tttaccaatt ttatccaagt gatttgtagg ttcatctaaa
    17941 agtaatatcg gactataatt tgatatgaca ttagctaatt tatattttgc tttttcacca
    18001 ccactcatac tgtcggttgt atgcataggt atatggagtt cactcattaa actaccgtct
    18061 aacgtgttaa aatcattttc cacatccatg ttaagctgtt caaaatattg aatttcgcca
    18121 accgtttcaa ctgttccaga ttctggtttt gttttttgag caatcatatt tagtaatgtg
    18181 gttttgccag taccattacc cccaataagt gctatttttt caaattggta agcgtaaaga
    18241 tgaccgatgt taagtgatcg taaatctgtc aatttatgat tgatttggtt aaatttaatt
    18301 gtatattgtt ccatatttat acactccttg tatatttaaa ggacgtgtac gcaaaaaaat
    18361 gagacaggct actcccgtct cattcacatt acactttcaa acacttaaaa atttattatg
    18421 atgttttagt atttgaaagc gtttattatt cctatcgaag gataaaaaat gacataagtc
    18481 atcctatgag tgtgtattgc ggacaagcct ttttgaatta atttagtttt gtattcaaaa
    18541 aggtttattt gagtctcata gaagctgtca ttttaatcct ccttcatcga taaaatagta
    18601 atgttaatat aacattttgt tggttttttt acaatatatc tctaagtaga aagattatat
    18661 aacaaaagta taaaaataca attttagatt gattatacat ctccttaatt tagagtgtac
    18721 tcataaaata cggttctgtt gcaaagttga atttataata taattttaac aaaaaggagt
    18781 cttctgtatg aactatttca gatataaaca atttaacaag gatgttatca ctgtagccgt
    18841 tggctactat ctaagatatg cattgagcta tcgtgatata tctgaaata
//
LOCUS       DAKTLH010000040        18526 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.040, whole genome shotgun sequence.
ACCESSION   DAKTLH010000040 DAKTLH010000000
VERSION     DAKTLH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18526
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.040"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            158..1084
                     /locus_tag="O0394_001170"
     CDS             158..1084
                     /locus_tag="O0394_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCY0499258.1"
                     /translation="MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     NLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
     gene            complement(1147..1581)
                     /locus_tag="O0394_001171"
     CDS             complement(1147..1581)
                     /locus_tag="O0394_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCY0499259.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(1597..2634)
                     /locus_tag="O0394_001172"
     CDS             complement(1597..2634)
                     /locus_tag="O0394_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCY0499260.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            complement(2961..3422)
                     /locus_tag="O0394_001173"
     CDS             complement(2961..3422)
                     /locus_tag="O0394_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HCY0499261.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            complement(3447..4358)
                     /locus_tag="O0394_001174"
     CDS             complement(3447..4358)
                     /locus_tag="O0394_001174"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCY0499262.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLFGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            4798..5709
                     /locus_tag="O0394_001175"
     CDS             4798..5709
                     /locus_tag="O0394_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HCY0499263.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
     gene            complement(5849..9301)
                     /locus_tag="O0394_001176"
     CDS             complement(5849..9301)
                     /locus_tag="O0394_001176"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCY0499264.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFNIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(9855..11081)
                     /gene="ftsW"
                     /locus_tag="O0394_001177"
     CDS             complement(9855..11081)
                     /gene="ftsW"
                     /locus_tag="O0394_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCY0499265.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
     gene            complement(11395..11670)
                     /locus_tag="O0394_001178"
     CDS             complement(11395..11670)
                     /locus_tag="O0394_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCY0499266.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            11811..12293
                     /locus_tag="O0394_001179"
     CDS             11811..12293
                     /locus_tag="O0394_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499267.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            12295..12462
                     /locus_tag="O0394_001180"
     CDS             12295..12462
                     /locus_tag="O0394_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HCY0499268.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(12602..14449)
                     /gene="typA"
                     /locus_tag="O0394_001181"
     CDS             complement(12602..14449)
                     /gene="typA"
                     /locus_tag="O0394_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCY0499269.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            14551..14742
                     /locus_tag="O0394_001182"
     CDS             14551..14742
                     /locus_tag="O0394_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HCY0499270.1"
                     /translation="MKQKKSKNIFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(14896..15723)
                     /locus_tag="O0394_001183"
     CDS             complement(14896..15723)
                     /locus_tag="O0394_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCY0499271.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWIVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGLHQEISNDYLEPHHDALIQLHEQRFKRKSK"
     gene            15880..16494
                     /locus_tag="O0394_001184"
     CDS             15880..16494
                     /locus_tag="O0394_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HCY0499272.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLRELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATNKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLIEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            16678..18030
                     /locus_tag="O0394_001185"
     CDS             16678..18030
                     /locus_tag="O0394_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCY0499273.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQS
                     "
     gene            complement(18112..>18526)
                     /locus_tag="O0394_001186"
     CDS             complement(18112..>18526)
                     /locus_tag="O0394_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCY0499274.1"
                     /translation="RKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAKQL
                     SQNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSLMA
                     LNLIAIVLWVIVMIMLISKKESKETTHKDDEYIYH"
BASE COUNT         6103 a         3312 c         2700 g         6411 t
ORIGIN      
        1 tcttatgtaa ttgctcgata aatgtattta gattatatta aacatcatta tattaattca
       61 attctcaaga gcatcactat atgattaacc acacatttcg ctgtagtcag aactatgaat
      121 ttgttaagct ataaaagtaa acgaggagga attttttatg aaaaaagtaa acagaaacat
      181 tttaaatgcg gtactagtcg gtgctggatt tttaagtagt ctattaatga tcaataaaaa
      241 taaagtgatt actaaaaagc aaaccatccc tgcttttttc aaaggcaatg caccatatat
      301 atttgcgcat cgcggtggta tggctttaag accagagcaa acgcaacttg catttgacta
      361 tgctaaacaa ttaggcgttg atggctttga aacagatgtg agactcacaa aagatcaaca
      421 gcttatcgtt tttcatgacg caactgttga tagaacgacg aacggctctg gaaaagttag
      481 tgcccataca ttagcagaat taaagaaatt agacgcagct tatcacttca aagatatcaa
      541 tggactaaca ccatatcgtg gtcatgcaca cacagctata ctaaccttcg atgagttgct
      601 gaagcaatat cctgatatgt atataaacgt agacttaaaa gatgctcctg aatcctatga
      661 aggctcaatc gcaccacaaa ttatgtttga tacaattgca gaaaatcaag catttgaccg
      721 cgttcttgtg acaagttttt ataaagaaca aatcgttcgt tttaataaaa ttgcacaagg
      781 atccgtcgca attggtgcta gccaacagga agttacagaa gcatttttaa aatatcattt
      841 attaggtggt aggtactacc aaccattagc acaaacgttc caaatgccta ctcattttaa
      901 aggcattgat ttgacttcat ctcgttttat caaatggtta aatgatatga atatcattcc
      961 aggctattat ggcgtaaata gtataaatct gatgaatgat ttataccaaa aaggtgcaca
     1021 cacgattgtg acagatcgac cagatttagc acaacaattc aaacaaacaa tcccgaataa
     1081 ataaaaacat cgagtcagat gctagttagt ggtttcaata accacctttt tctgactcga
     1141 tgttttttat aatgaacaat tacaggatcc gcccgtcgca caaccactat gtgatgtttg
     1201 aaagaatgga ttacctgttt ctattttgac atgttctgaa acagcgtacg caattttcgt
     1261 aacaacttca tctattaaat gttgaagtgc catttcccta gctttgtaat gcatcacaac
     1321 gtcaagcatc tcatatgctc ttttgcgttg tcttgtctct aacataactt tcttataatc
     1381 aggatgatat ttcccgaatc gcattacttc atcatatttc tcttttgatt ttaaaaatga
     1441 ttgataaagt aaatgtgctt cgtcattgtt ttcgagcgcc tgtttagctt gctcaaatga
     1501 tgcataaata tctgatgcaa ctatcatatc agctagttca tcaatatcat ctaatatcgc
     1561 tagtgaagct tcattaatca taaatacccc tcattgttat ttttctaaga aaattagtga
     1621 ataataatct ctcattacat ctccacccat ctcatcgtat tttgaattta atagacgaga
     1681 tcgatgtata tctgaattca tccaactatg gattaatgtt ggtacatcat taaacgcata
     1741 accaacattt tgagcagttg ttttataagt aacgtgattt ttatctaatt gccctcttaa
     1801 tgcgtcctct gtaaattcaa cactatcaga accattagag gtcgcttcat ataagttatt
     1861 agatgcaata tgtgctaaat cgctattgat tttcaatggt tttaatcctt ttaattttct
     1921 catttcattc gttacttcat aaagagaaat taattgattt ggattttgct caactggacg
     1981 cttattatgc tcttctgacg tagaattaga atttaattga taaggttcaa tatctgctaa
     2041 catttctttt gttaaaaatc gtacacttag cacctttttc gattgttgat cagaatacac
     2101 ttgtgcatat atgtcgccat atttaatcag tgtttgtgtt tttaaatctt catctgaaag
     2161 ttcaaattca tattttttac catcaacttt aaaggacggt tctggattaa tacttgtatg
     2221 attaaaaatt tctgcagaat gttgtcctat ttttaacgga ctaacattga ctttctcacc
     2281 tgtagcatac actgaaacga tttcttcacg tttagttgaa acaatgtaat aactgttttt
     2341 gtctttaaac acataatttt tgtaaccatc tctaaaaggg tagacacgat ctgcttgtcc
     2401 aaatttagaa gtaagcactt taatatcttt acctacccat gtaccaacac cttctttagg
     2461 ctttggattt tcagcatggt tatttgattt attcacctgt tcagatctat ttactttatt
     2521 acctttattc gggttctcta atacatcaaa ttttaatctc ggtgaataaa aaagatatat
     2581 taaaaatcca actaaaaata atacaccgac gactcttata attaactttt tcatcaatca
     2641 accacctaaa aagtattaat actattgtaa aaaacaacac attaattagc aaattttcaa
     2701 cactgacata actgtgtcgt ttcgataaaa caaaatcatc ttcaggcata attttaacgt
     2761 cataaagatt ttccctacac tctatatcat aacctatctt tatgttttca ggttgaattt
     2821 cattattaag attaaaatat gtataaaaaa atggacagtt aaggtatcaa atgaatacca
     2881 tcaactgtcc gactattctt catcaaaaaa cctgataaaa caaattgcct tatcagatta
     2941 gtatcatttg tataagcata ttaatgacca aatgttgctt taatcagtga tgtcgtttct
     3001 ccacctggat ataatacata taataataag taaactgcta cacctgtaat tgcagtacag
     3061 aaccatataa ctgaagcgaa tggcccgaat ttgcggtgta cattatattt atctttaaat
     3121 gcagtaataa tttgaactag gcctagaata ccaccaattg ttgctaaatt aatgtggaaa
     3181 aataagaaaa tcgtataata tttcttaatt gatgctggtc cgccaaatgc tgtattaccg
     3241 ataaaaatcg ttctacttgc atagattaag aaaaaagtta aagcaaaaac agctgcagct
     3301 aacataacat ttttgtgttt atttatttcc cttttccaaa taagtctcca accaatggca
     3361 attaaaattg cactaatgac aatacatgtc gtactaatcg ttggtaaaat tggaacgccc
     3421 atatttttca tcctaactta attaatctag atcaaagtaa gtaatgaaac aatcacagct
     3481 aacacgaaaa agatcactaa ataatttagt gaatatataa acatttgtgt tgcccatttt
     3541 gtttgatctg aatttttctt aaatgttgtt aaacctaatg caatccatcc taaatttaat
     3601 aatgtagcta acactacgaa tacgacacct aaatttatta gtaataaagg tactggcaat
     3661 aaaataatca accagataaa catactgaca cgtgtacgtt taaagccctt aactgatggt
     3721 aacattggaa tatttgcaag tgcatattca tctttacgtt taatagctaa ggcataaaaa
     3781 tgaattggtt gccaacaaaa tacaactaaa aacagcgcaa tcgctgttaa actaatttgt
     3841 ccttcaattg caacccatcc aattagtggt ggtactgctc caggaaaact cccaatcact
     3901 gtgttccatg ttgtatgtct tttagaccat attgagtaat aagacacata acctacaatc
     3961 cccataagac caagtacgcc tgatggtata ttcaataaaa acaaacaaat ttctccaact
     4021 aacatcatac caaaacttaa tagtaataaa ttttgatctg taattctatt atttacagtt
     4081 ggtctatttt gtttactagg cataatacga tcaatatctt ggtcgtaata attatttaac
     4141 gcacatgcgc cacccataat taaagtagat ccaaatagca ttaataaaat ttgaggtatt
     4201 gatgataaga aggaatgatt tgtcattaca actgctagcc atgcgcccgc aaaagccgga
     4261 attaagttac cttgaacaag tcccatttta attatctgtt gcaattcttt gaagttaact
     4321 ctgctaatat tttgtgacaa agtatgctct ttgctcataa tccccctcct taaatttgtt
     4381 catataagat tatgatatct tagattgcat aaaaagacta ggtttaataa aattaaattg
     4441 tgacaaatta acgacaagag aaaatgtcaa ttttgtgaca caaataacat ttaatttatt
     4501 gctataatgt atatgttaga aaattttaat aagtagaatc atgcatctaa aagagattaa
     4561 tatttaagct tcaaatttga gtaaacgtgg attacataat tatcccaata aaaaaatcat
     4621 tacgattaag ttctttttat gtcgtccaca tacaatactt gtaaaattaa atcatatttc
     4681 ctgcgttgga tcccatcttt tcatatccta caatcaggtc tatttatagt atcatctcaa
     4741 atccggctat taattctaat tctcagtgat gcgttttttt atgatggggt gtataaattg
     4801 tttggcaaaa agaatttaaa atggttaggt gtcgtagcaa cgttaatgat gacatttgta
     4861 caacttggtg gagccttagt taccaaaacc ggatcagctg atggttgtgg ttcttcttgg
     4921 ccactatgtc atggtgcgtt gattccagaa ttctttccta ttgatacgat tattgagtta
     4981 agtcatagag ccgtttcagc tttgtcttta ttaatggtct tatggttagt tatcactgca
     5041 tggaaacata taggctatat taaagaaatt aaacctttat caatcattag tgttggattc
     5101 ttattattgc aagcattaat cggagctgct gctgttattt ggcaacaaaa cgattacgtt
     5161 ttagcattgc actttggtat atcattaatc agtttctcat ctgtattttt aataacattg
     5221 attattttct ctatagatca aaaatatgaa gctgacgaat tatatatcaa aaagccatta
     5281 agacgtttaa catggttaat ggcaatcatc atttattgtg gtgtttatac tggtgcacta
     5341 gtgagacatg cggatgcaag tttagcatat ggtggttggc cattgccatt ccacgatctt
     5401 gtaccacatt cagaacaaga ttgggttcaa ctcacgcatc gtatcatggc ttttatagtg
     5461 tttacgatta ttatgattac ttatattcac gctgttaaaa attatccaaa taaccgtact
     5521 gttcattatg gttatacagc tgcatttata cttgttatat tacaagttat cacaggtgca
     5581 ttatctatta tgacaaatgt taacctgata atcgcacttt tccatgcatt atttatcact
     5641 tatttatttg gtatgacaac atactttatc atgctaatgt tacgatcagt aagaagtgac
     5701 aagcaataac aaaaaagcaa accgtaattt taatggcacg cccattaaaa ttacggtttt
     5761 ttatatcaat atttaaaatt aaacctaagc catgtaaaaa cgagattaca cgtcaattgt
     5821 tgtgtaatct cgttttattt taatcatttt agtcagttgc tttttcaatt tcgattaata
     5881 aatcgcctgt cgctattgtg tcaccattat ttacagttac ttgtttaatc acaccgtcaa
     5941 atggtgcttg aattgttgtt tccattttca tagcttcagt aattagcaac ggctgattag
     6001 ctttcacagt ttcacctaca ctaaccttga cttcagttac tgaacctggc atttgagcac
     6061 cgatatgact tggattactc ttatctgctt ttggcttaac gttcgcattt gtatgcacat
     6121 tttcatcttt aatgtaaata cgtctcgctt gaccattcat cgcatagtaa atcgttctat
     6181 taccattttc atctggttca ctaatcgttt ctagtttaat aattaatcgt ttacctttat
     6241 cgatttcgat ttctactgtt tcaccattac gcattccaaa gaagaatgta ggcgtatcaa
     6301 gtaacgataa gtttccgtat tgatttctag tttgaatata ttgttcatat acttttggat
     6361 atagtacata actaataata tcttgctccg taacaggacc ttgttgctct tcttcaagca
     6421 actcacggac tttttcaaaa tcaactggct ctagatattc acctggacga gctgttagtg
     6481 cttcttggcc ttttaaaata accgcttgta aatctttatt aaaaccattt acaggttgtc
     6541 ctatttcacc tttgaagaac gacactactg attctgggaa atctaattta tagccatctg
     6601 taatcacgga ttgttcatca agatcatttt gtaccatata aagtgccata tcaccaacta
     6661 ctttagacga tggtgttact tttacgatat caccaaataa gaaattcact ctgcgataca
     6721 tatctttgac ttcatcaaat ctttcgccta aacctaaact tttagcttgt tgacttaaat
     6781 tcgaatactg tccaccaggc atttcatgtt gataaatttc agtattcggt gatttgatat
     6841 cactttcaaa gtctgaataa taagtacgta cagttgacca ataatgacta agtgactcca
     6901 taccttcaat atcagttcta aggtggcgtg ggaagccatt taatgcataa tataacgaat
     6961 tggcgcttgg ctgacttgtt aaaccactca ttgaagcaac agcagtatca atgatatcga
     7021 caccagcatc tattgcttgt ttgtatgtta ataaaccatt accacttgta tcatgagtgt
     7081 gaagatgaat tggtaaatct acagctgatt ttaactcacc aatcaattcg taagcggctt
     7141 taggttttaa taagcctgcc atatctttaa tcgctaaaat atggaaacct tcacgttcta
     7201 actctttagc tagtttgaca taatactcta aagtataaat gtttgatcgc tcaggattta
     7261 aaatgtcacc tgtataacaa atagtacctt ctgagatttt gcccgcttct tgtactgctt
     7321 cattggcaac tttcatttga tctacccagt ttaatgaatc gaaaattcta aagacatcta
     7381 tgcctgcttt agcactttct tgtacgaatt tatgaataac attatcagga tagtttttat
     7441 aaccaactgc gtttgaagca cgtaacaaca tttggaataa tacatttgga atagctttac
     7501 gtagacgttc aagtcgttcc catgggtttt ccttcaagaa attatatgcc acatcaaatg
     7561 tagcaccgcc ccacatttct agtgagaaac catctttaaa tacgtccgct gttttggatg
     7621 cgatattaat catatcctta gttctaactc gtgtagctaa taatgattgg tgtgcatctc
     7681 taaaggttgt atctgttagt aagacatcat cctgcttttt aacccattca gctacacctt
     7741 ttggacctac ttcatcaagc aattgtttcg taccactaaa tgaagcgatt ttacttgaag
     7801 atacagttgg aattgatgct aattcatagt ctggtttcgg acgtttctca acatttggga
     7861 aaccattaat tgttacatta cctatatatt ctaatgtttt agtacctcta tctagagacg
     7921 gctgaatgtt gaaaagttct ggtgtttctt caataaattt agttgtgtaa tcaccacttg
     7981 tgaacttttt attcttcatt acattaatta agaatggaat attagtttta acaccacgaa
     8041 tacgcatttc tcgtaatgag cgtaccattt tttcttctgc ttgtttaaat gatatcgcgt
     8101 gtgtagataa ttttacgagt aatgaatcat aataaggtga tatctcagca ccttggaaac
     8161 catctccagc atcaagacgt acaccaaagc ccccgcttga acgataagca atgattgttc
     8221 cagtatccgg catgaaatca tttaacggat cttctgttgt aatacgacat tggatggcat
     8281 agcctaatgt tgtaatatct ttttgttgcg gcatattaat ctcttcacca aataaatcgg
     8341 cacctgctgc aactaaaatt tgtgtcttaa caatatcaat tcctgttacc atctctgtaa
     8401 ttgtatgctc tacttgtaca cgagggttaa cttctataaa gaagaattcg tcaccagata
     8461 ctagaaattc aacagtacct gcattgacat atttaatatt ttccatcaat tgaattgcag
     8521 catcacaaat acgttgacgt aatgttgatg ataatccaac tgatggtgca acttctacaa
     8581 ctttttgatg acgacgttgt actgaacaat cacgttcaaa taagtgtacg atatttccat
     8641 gttcgtcacc tatgacttgt acttcaatat gctttggatt atcaatgtat ctttctatgt
     8701 aaacttcact attaccaaat gatttttcag cttctgattt tgctctatgg aaagcatctt
     8761 ctaattcact ttcttcacga acgattctca tacctttacc gccgccacca cttgtggctt
     8821 taatcattag cgggaaacca gcttcttctg caaattcttt tgctaattca tatgatttaa
     8881 ttggaccgtc tgtaccagga ataactggta aatctgcctt gatagccgtt gtacgagctt
     8941 taactttatc tccaaacata tctaaatgtt ctaaatgagg accaataaat ttaattcctt
     9001 cttctgcaca acgacgcgca aattgttcat tttcacttaa aaatccatag ccaggatgaa
     9061 tcgcatccac attcgcttgt tttgctacat caatgatacg ctcaatattt aaataacttt
     9121 cagcaggacc taaatcactt ccaactaaat aggattcatc tgctttatat ctatgtaatg
     9181 aacttttgtc ttcattcgaa taaattgcaa ctgtgctgat gtctaattct gccgccgctc
     9241 tgaatatacg aattgcaatt tctccacggt tagcaacaag taactttttt atttgtttca
     9301 atagcgatac actcctcaaa ctattagaat tttctaacta attagataat aaaattttat
     9361 cttaaagcgc tctgttttgc tatagttatg tttcaaattt tcaaaattta acattcttga
     9421 gacaattaaa acctccgctt cagaaataat aatttcaaaa atgactatgc aacaacaggt
     9481 agttcacgtt tttgttgtga aacattttcg atttctacaa ctctaaaaaa ttaaaaataa
     9541 aattgcaaaa catcaacatt tattatcaat agcgataact ttatcttatc atcatgattc
     9601 taatttcgcc accacattta gtaattttta gtcataaaat ttagttataa ttatacgttg
     9661 ttttgtttat aaaatttgat aatcatgagt aatctcgtaa tatcaaaaca aaaaggaagt
     9721 taagcgttgt ttggttgcct aacttccgtt attgaactca tcagtccaat ttgacaaaga
     9781 gtatataaat agccaatgtt tagcattgct taatgacaga agtcatctcg tactaaatat
     9841 tggctagtat ttttttaatt aaattgtctt cttatatcaa ctttttgttg ttgtttcttt
     9901 cgttgctggt ctactttgat ttgtttacct acaatcagaa gtaaacccat agcaatactt
     9961 aaactaatca ttgatgatcc accaaagctg ataaatggca atggcacacc agttaatgga
    10021 attgttgccg aaataccgcc aatgtttaca aacgtttgac ttccaaagta tgtggcaatc
    10081 ccaacacaca caagtttata aaaatatgat gatgttttat ttgcaaactg gaaggcacga
    10141 tatacaataa agaactctaa agtaataact agcaatcctc cgattaaacc taattcttcg
    10201 caaataattg caaaaataaa atctgtatgt ggttctggta aatagcccaa tttcattgca
    10261 ctatttccta atccttttcc aaatacgcca ccgttaccta tcgcaagcaa tgaattggaa
    10321 atatggtatc cagttcctga ttcgaattgg aatggatctg ttagcgtact aaatctggca
    10381 gttaaataac ttggtaacca accagccatt aatgcaatga caaatactac taagaatcct
    10441 agcactgctg gtataccaaa tcttaggact ttgtttaccc caattcctga ataaaaaatg
    10501 atcgcaacta aaataattaa tattagtaat gtttgcccta cgtctttttg taggaaaact
    10561 aaaaacgtac aacctaatgc taatacaata ggacttaaaa ttaattttgg tttacttaat
    10621 actctaggca tttttttact gatcataaat gggatatata atatgattgc aatttttaat
    10681 aactcagatg cctgtaagtt cataaatcct aagtttatcc aacttttaga accattaata
    10741 tctttaccta ttactaacgt cagtaataat agtgagacga tagttataat catacctttt
    10801 tgcactttaa tattactcag taatttaaca ttcattaaaa atgcaataaa aaatacaatt
    10861 ataaaactca ttatgacata tgctaattgt cggttgtaaa aatacgttcc tggaacatcg
    10921 ataccaccag tcaatgtgcc tttagttgct ggaaccatac ttgcactata taccatcact
    10981 aatccaatta aactcaatac aatatatgta actaataacg gataatcaat aaactttgag
    11041 gttttaccaa tataccgtaa aatacttcta aaattcttca tatattcagt catccaattc
    11101 tacaatttat acttcaaatg atttcgtttt atactttaat tttaaaaatc catgttactt
    11161 attatataca tgacacagtc ataatcacaa atattaacag gtctcttgag gtaatttata
    11221 gaatatttgt ttacttaata tcgtgaattg ctaaaaccca ataaaagtga ctgacatgtc
    11281 atcaagaaga caacataaca gccactttaa tcagtaatta aactataaat ataaaatcgg
    11341 cgcagctcaa ttgtcgatat ctcacgccat tttaatatgc ttatatatta aaccttaaac
    11401 aagtgtaaaa gcttcatgta attttgaaag ttctttctca agacgtaaca taatttgttt
    11461 gccatcttcg cggtcaacta aacctaattt aacagcaaaa tcaacttctt tttgaagtcc
    11521 aaacatttgt gtatctagta cttcttcata taatgggcat gaaggtaatg ttaaattatc
    11581 tagttgaact ttaatcagat gcaagatttc atcagcatct ttagtcaatt gtttcaaagc
    11641 tgcatttttc attgttgctt gtttcgccat gacacgctcc ccctctatat aaattctcaa
    11701 gtcttactaa aagtttatct tactaaatat taaaaagcaa gtagatttaa aatgtgtcgt
    11761 tagcaatttg tgttaaaata atgctatata cattgatagg gagtgggaaa atgatacaaa
    11821 ttaaaggagc cgtaaagttt cctataacat tagatagtac gacttggatt ttcgatgata
    11881 gaaaagttag catcgaagat ttagaaaaag gtgtatttga tggttcaaaa ccaatcgact
    11941 ttgaagataa tcatgaatgg aatcgtgcta tcctagaagg acaaactaat ccgcctactt
    12001 taaattcaga aataaaatat aaaaaacgtg ccatgttaga aggttcattt gtcattaata
    12061 tgacaccatt tttcaaacat tccgaaccac atgactctgc aactactatt cgattatcaa
    12121 atgagcatga tcatttagat ataccgatag atctattacc ctatttattt ttccaatttg
    12181 caaaagacgg taaacgactt tacagcgata atggtgtaga tagttttatt tacaatcaag
    12241 aagatggtta ttcatatcga atcaaccatg taactcatat tgaggtgatt taatttgaga
    12301 caagtgcaat gcattatctg tgatgcaaaa gtatttatag atgagcgcac aactgagtct
    12361 aaacgactta aaaacaatcc tattcgaaca tttatgtgtg atgattgtaa aagtcgatta
    12421 gacacaccta aacaacgtgc gcaacactat ccgctcgatt aatatctgaa taaacaagtt
    12481 agaaatttga aaatgtgaaa gtaacaggaa ataatagcat ttaaacactt tttcaaatat
    12541 tatatgccaa aagagaagct aagcaacttt catcgctcag cttctctttt attatgttaa
    12601 attattcgtt ttcttgcatc atttgtttga tacgctttgc ttctttttca cgaacatttt
    12661 tgtttaaaat tttctttctt aaacgtatac tttctggtgt aacctcaaca agttcatcat
    12721 cattaatgaa ttgtaacgct tcttccaatg ttagaatacg cggtctattc attgtttgtg
    12781 tttggtcttt cgttgcagaa cgtacgttag tttgatgttt tgttttagtg atgttaacag
    12841 ttaaatcatt ttcacgatta tgttcaccaa caatcatacc ttcataaact tcagtaccag
    12901 gttccatgaa gtttacacct ctatcttcaa gtcccaaaat ggcataagta cttgcagaac
    12961 cttgatccat tgaaattaat gcaccattac gacgaccgcc aatttgtgct ttaatacgtg
    13021 gtctaaattc ttcaaatgta tggttaataa taccgtaacc tcttgtcatt gacataaatt
    13081 cagtcgtata accaatcata ccacgagccg gtacattaaa gattaaacgt gtaagtccat
    13141 tatcagttgt agtcatatca accatttcac ctttacgtgc acctaatgat tcaataacag
    13201 cacctgcatt ttcttgtggc acttcacatt gcacacgttc aaatggttca cacattacac
    13261 catctatttc tttaataatt acttgtggtt ttgaaacttg taattcataa ccttcacgac
    13321 gcatattttc aataaggatt gataaatgca attcaccgcg accagcaact acccatgtat
    13381 ctggagaatc tgtgttagaa actttcaaag atacatctgt ttctaattgt tgatttaaac
    13441 gttcttgaat ttgacgtgct gttacaaagt caccttcacg gccagcaaat ggagaattgt
    13501 taactttaaa tgtcatttca agagtaggct catcaatacg tagaactggc aatgcttctt
    13561 gatggtcatg tggtgttaca gtttcaccaa cattaatgtc ttccatacct gaaacagcaa
    13621 ttaaatctcc agcttgtgct tcttcaattt ctaaacgttt taatccaaag taaccaaaga
    13681 ttttagttac acggaagttt ttcactgtac cgtctaattt aattagtgat acattatctc
    13741 cgacacgcat tttacctctg aatacacgac caataccaat acgtccaaca taatcattgt
    13801 agtccaacaa tgctacttgg aattgtaatg gctcatcact gttatcaatt ggagctggta
    13861 cataatcaat aattgtttca tataatgatt gtaaattatc atcttgcttt tcaggatcta
    13921 agctagctgt accatttact gctgaagcat aaacaacagg gaattctaat tgttcatcgt
    13981 ttgcttctaa ttcaataaat aaatctaaaa cttcatctac aacaccctct ggacgtgctg
    14041 atggtttatc aattttatta acaacaacaa caggtttcag gttttgttct agcgcttttt
    14101 taagtacaaa acgtgtttga ggcattgtac cttcatacgc atctactact aagacaaccc
    14161 catcaaccat tttcataata cgttctactt ctccaccaaa gtctgcatgt cctggtgtat
    14221 ccaaaatatt aatacgtgta cctttataat caacagccgt atttttggct agaatcgtaa
    14281 ttccacgctc tctttcgata tcgttagagt ccattgcacg ttcatcgaca tgttcatttt
    14341 ctctgaatat accagattgt tttaacaact catctactaa agttgtttta ccatggtcaa
    14401 cgtgagcaat aattgctata ttgcggacat cttctctttt attagtcata taaatagttt
    14461 ccttctttct taagtaaaat cccactttta attacaactt ttttattata tcatataatt
    14521 gagaaaagat aagcaaaagg tggggatagg atgaaacaaa aaaaatctaa aaatatcttt
    14581 tgggtatttt ctatattagc tgttgttttc ttagtattat ttagttttgc tgttggtgca
    14641 tcaaatgtac caatgatgat tttaacattt atattactcg ttgcaacctt tggaattgga
    14701 tttactacaa agaaaaaata tcgagaaaac gattggctat aagttatatc gacaataaca
    14761 cgcgttgatt taagaccaca catgttcatg atgaacattt aactggtttt atattggcgc
    14821 gtgtttttat tatttaaggt ataaaagcgt aatatgttac ctaattgtta ggactacata
    14881 ttacgctttt ggtaattatt ttgattttct tttaaatcgt tgttcatgta attgtattaa
    14941 cgcatcatgg tggggctcta aataatcatt gctaatttct tgatggagac cacgatttcc
    15001 aactaagatt gaatttggac cactaattgt taatggttct cctagtaaat tggaagcttg
    15061 tccatttact tcatataaaa taaccaatcc gccagcaaaa tcccatggtt gaagtcttgg
    15121 cgtcatatat gcttctaaat tacctgtagc aactgaaacg atttcaagcg ctgcactacc
    15181 atatgccctt gcacttctag aatcattaac aatttcttta aagatttctc ctaaaattgg
    15241 tttagttaac cagttcggat tgatcccaat aatgctttgt cttagattag aatcattcaa
    15301 tggtttcaag ggttggctac cacgatatgc accttcccct actttagcat gatataagac
    15361 atcagccata acatcatata caaaacctgc ataaggttta ccatcgatat aaataccaat
    15421 tgaaattgcg aaattttctt gttgatgaac aaaattcaat gtaccgtcta ttgggtcaac
    15481 aatccatacc gtacctttgg aagtatcgat gtcatgacca tgcccttctt cacctaatac
    15541 ttgatgattg ggatatgttt ctaaaattgt atcaaaaatg aaatcttctg ttgctttatc
    15601 tacatttgta acaaggtcat tcggatttga ctttgtttca attgttaaat tttgctccat
    15661 caattgttta attctaattc ctgcttcttg aataagtcct tgggcaaatc cataaagtgc
    15721 cataacatca tctcctaaca tctttattat acatcaacat tttataaaaa taacttctta
    15781 tgataaaaat gaaaatacta attttaatga gagcgtctta agccgcaatt gataaaacat
    15841 atgctacaat atttttaaac actatacagg aggtactcta tgacaaaata tacatttaaa
    15901 cctaaagatt tcaaagcgtt caacgtagaa ggcttagacg cacgaatgga agctttaaac
    15961 gaatacatac gaccacaact ccgtgaatta ggagaatatt ttagtgactt ctttacaagt
    16021 caaactggtg aaacatttta tcctcacgta gcaaagcatg ctagaagaag tgtgaatcct
    16081 cctaaagata catgggttgc ttttgcaaca aacaaaagag gctataaaat gttacctcat
    16141 ttccaaattg gtatgtttga agatcaactg tttgttatgt ttggaatcat gcatgaagca
    16201 aaagataaag caacacgtgc aaaagttttt gaaagaaaat ttaaagctat tcaacaatta
    16261 cctgatgatt atcgtgtttg cttagatcat atgaaacctg ataaaccatt tattaaagat
    16321 ttaacggatg atgatttaat agaagcgata caaagagcca tcaatgtgaa aaaaggtgaa
    16381 ttctttatag cgcgtgcaat cacaccacaa gataaaagat taaaaagtga caaagcattt
    16441 attgcatttt tagaagaaac cttcgatcag ttcttaccat tttattctgc ataaataact
    16501 ttgtttaaat aatagagcac ttaatcacat ccatgatttc gtgctctttt ttcttaatat
    16561 taaatcgaac gttcaacata ataattcata cttttaaaaa aattaaaata aatttaggtt
    16621 gacctaaaca ttttattagg ttattatatt gtccataaga agtagaggtg agtcaaaatg
    16681 aataataaac gacattcaac aaatgaacaa ttaagtttag acgaaataaa caatacaatt
    16741 aaattcgatc atcgcagttc aaataaacag aaatttttat catttcttgg acctgggtta
    16801 ttagtcgctg ttggttacat ggatcccgga aactggataa catcaatgca aggtggcgcc
    16861 caatatggct atactttgct attcgtaatt cttatttcaa gtttatcagc aatgttactt
    16921 caaagcatga cagtgagatt aggaatagca acaggtatgg acttagctca aatgacacga
    16981 cattatttat caagacctat tgctataatc ttttggatca ttgcagaact agcaattatc
    17041 gctacagata ttgctgaggt tattggtagt gctattgctc ttaatctcct atttaacata
    17101 cctttaatcg tcggtgcact aataactgta cttgatgtat ttttactact ttttataatg
    17161 aaatatggtt ttagaaaaat tgaagctatt gttggtacat taattttcac agtgttattc
    17221 atctttatat ttgaagtcta tatttcatca ccacagttga atgctgtgtt aaatggattt
    17281 ataccacata gtgaaatcat tacaaataac ggcattctct atattgcatt aggtattatt
    17341 ggcgctacaa ttatgcctca taatttgtac ttacattcat caattgtaca atctagaaca
    17401 tactcaagac ataacaatga agaaaaagcg caagcgatta aatttgctac gatagattcg
    17461 aacattcagt tatcaatcgc atttgtagtc aattgcttat tattagtgtt aggagcatca
    17521 ctatttttca actcaaatgc tgacgattta ggtggtttct atgatttata tcacgcctta
    17581 aaaactgaac ctgtactagg tgcaacaatg ggtgcaatca tgagtacatt atttgcagtt
    17641 gcattattag cgtcaggtca aaattcaacg attactggta ctttagcagg acaaattgta
    17701 atggaaggat ttttaagatt acacatacca aattggttaa gacgtttaat tacacgttct
    17761 cttgctgtca ttcctgttat cgtatgctta atcattttta aaggtaatgc agctaaaatt
    17821 gagcaactac ttgtattctc tcaagtattt ttaagtattg ctctgccatt ctgtttaata
    17881 ccattacaat tggctactag taataaagac ttgatgggac ctttctataa taaaacatgg
    17941 gttaatatca tttcatggac acttattatt attctaagta ttttaaatgt ctatttaatc
    18001 gttcaaacat tccaagaact acaaagttaa aaataaaacc attaaaatgt tggttgtatc
    18061 taataattaa caacttaaca ttttaatggt ttttcttttg ctatgaacat attaatgata
    18121 aatatactca tcgtccttat gtgttgtttc ttttgattct tttttagaaa tcaacataat
    18181 catcacgata acccataaga caattgcaat taaattaaga gccatcaaac ttacaattat
    18241 cgcgataaca aaaagtataa tcgataattt tctacgtcct ttaatacaaa ttgtcgcaaa
    18301 tataccataa agtgttgaaa ttaataaaat aattgctaat gcacccattg ttgtctgaag
    18361 catatctaaa gaaaccttgc caccgttctg agataattgc ttcgctaatt cttcacgttg
    18421 tgatgcatta ccacttttta aagaaaaata ggatagcccc ataactaata aataaataat
    18481 actaatacta tttgcaatcc aagccatgat taattcaggt tttcta
//
LOCUS       DAKTLH010000041        18462 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.041, whole genome shotgun sequence.
ACCESSION   DAKTLH010000041 DAKTLH010000000
VERSION     DAKTLH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18462
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.041"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..178)
                     /locus_tag="O0394_001187"
     CDS             complement(<1..178)
                     /locus_tag="O0394_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499275.1"
                     /translation="MRRWFVLILGLVILLSACGQKYDKEIDEVTKLEKESIQDVKNTK
                     KYKNVERSKSYYKIY"
     gene            complement(234..824)
                     /locus_tag="O0394_001188"
     CDS             complement(234..824)
                     /locus_tag="O0394_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499276.1"
                     /translation="MKQKIIMFTLVTVILFCAVLIGYQIPKQQVKMKQNQIEDLQEEQ
                     RILRDKNGELNKLVKRQSKTVISDEEKQIREVSSNFVKQMFEMKKDSSFKSKAPQIKP
                     LVTKDYYDTLFKDSKDKYDLYDDITVNDIHVYFDTYDPKKDSYKVFVQFDERIETDGD
                     DKIEHRQTSAQLDLVRTAEGWRIDNLKRFNLKPLGR"
     gene            complement(831..1877)
                     /locus_tag="O0394_001189"
     CDS             complement(831..1877)
                     /locus_tag="O0394_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0499277.1"
                     /translation="MVNKKDIAKKVIGKTPIGVKLKLAKVIIILCVVAFFIFPVIIMF
                     LLAPDLNKGKDDAGCTVSGGNVSANGIDKFNENAKGGKLEGKGKEIQKIAEKNKVPVN
                     IFMAIIASESQWGKGENATRQNNPLSVMGSKSIHDSTYPTIEDGLNAGAKNLYDVYIS
                     KGLDTPKKIGPKYAPVGASNDPNNMNARWIPTVEKIMKDLGGSEAKTSCSNGKGKSIK
                     FNGKLPHWSNDDPGKGNLYTAGQCTWYAYGMRQKMGKPVSTYWHDAHKWNDRAKAEGY
                     KVDKNPEPGALFIAEQGAGGHDGHYGHVAVVIGVSDGGKTFRITEMNWEGAFKVNERT
                     LKMTDGYSFIHDKE"
     gene            complement(1867..3714)
                     /locus_tag="O0394_001190"
     CDS             complement(1867..3714)
                     /locus_tag="O0394_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499278.1"
                     /translation="MKHKSVYLLCFLILPILLLTSVSAYAVSNPVGEPSHAVKPKIEK
                     YDLSHYRSIYSEKGDWNPFGQEEISRQINNVSDFFFSMTKILAGVTDYAIENLFQLDV
                     INDFADKIGDFVGDIYQKLLSNLALTLFIIVCFNAFIIFSVQGNAREALKRAFLIMCL
                     IGFGVGILANAGNIIRGTNNIGKDLNNIIMNSTSSIDGNIDYIHENSGMNHIRNQLFD
                     MTIYRTYLVMNYGTVDEKEIKAKGKDRIDNILKLDYDKKSEKELDDIVKDEVEKKNNK
                     YMTQGYVFQKLAISVIGFIITLFMSIVFLSISFAKLIFSTFALFLFLFLVFSWIVSFI
                     PGFELSVFSAFAKTLGYIILSACMTFLFVIVGLCIKLANSFIEPDSQNAYFLNSIFII
                     VILFVMYKKRAQIINFVSRGNISFSPSAIGAGVMNRTQERFNKIRHEQAQNKKAKREN
                     QRDEPAPPLQNDNDLRRRQQDKPMPLFINKDNQKNGNKRREQQESMNGNDVKSASVES
                     NANNYSKQPQKASQQEHQVRETRQRKDIQRSPQVVNQPLNNENHSINRKEQKSVQTAY
                     DTDVQKRQIQNATQNQQSRQSGNRNQPITRNSQSKDRLKEQKDINKHGK"
     gene            complement(3719..5077)
                     /locus_tag="O0394_001191"
     CDS             complement(3719..5077)
                     /locus_tag="O0394_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCY0499279.1"
                     /translation="MAMNEIALFKGVRIQPYQKYIDFMFASVLAFIFLVYRIYILFID
                     YKSVNKKLDILTLFAYFKPHLLYLLIGTAIIFVICKLIAQFLIRFKTKDLAEMIETQG
                     FHNRTVIKKTTEYIPIDYKQTEYDYYPTMFYKRGKKSFVIHIKKDGSRFQDNYLELET
                     IIEPMFNSELVEKKHIGRYLRYEFMPLKYKKRIVMNGEQESNTIFYDTKIAITHQITW
                     DFVKAPHGLVTGITGGGKTYFLFYVIRELFRRHSEVRLLDPKVSDLSFMKRVIGDDKV
                     ADTKGQILKQLREANNEMEERFRLMNDSSDYKIGNDFRNFDMRPYFIIFDEVTAFTST
                     LDKKELQEMNDYLINIIMKGRQAGVFMFLTAQRPDADVIKGNVRDQLGLRVSLGNLSN
                     DGYRMTFGQTDKEFQTIHDSDIGRGYISILGQYNEPILFDAPLMEQYDFVEDVKQILN
                     KE"
     gene            complement(5131..7626)
                     /locus_tag="O0394_001192"
     CDS             complement(5131..7626)
                     /locus_tag="O0394_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCY0499280.1"
                     /translation="MNLKAPYAGLRDNLLLTKTGEVWAYYRVRSESISTANYDAKEES
                     KKRMRYFLESIKGYQDFHFEMYDHDLDLRNKFKEISKDFDDEAFNVAEYYSKETIDVL
                     EQELQMITHNSFVMGVKIRELADDAVTLKEILKSTLSDTAERIVSNFGFDVNLDDKIL
                     DKYKTFERDLADSFLGIGGERLTENELAYIIRKPFISNATHDVQEESSRRAITDIYNA
                     VIDPTRLSVLEVENDNEKFYTTTVVVDDFPLDMEYTHLFEKAQNMPFPVECHVKAKII
                     NNEKVKKKFDRAKINHKQQAKEKQDTGDSASEDVQDNLFVLNQMEREVTGSGVFIEWV
                     FMFVIKSDDYRDCKRKAKRLMKRLKETQIYAVNPLADQLQLFYQCLHGNDLFINKYWL
                     QRTTATGIAENLFGVSQSLGTKTGFYIGRIDKFIRSVSREEAVASSRDIILFSLLLAA
                     KGIKGAVSDSPHVLITGQTGKGKSFLAKLLWIYTSFFKGQMLYWDPKSEFAEWFDRVT
                     ESKEMQEKYPLFINHLKTFKYVTLNYEDSTNYGCLDPIVFLDGIEANEMLKSVFDEIK
                     SFENYHHIETAIYQAISDTVEERENGKKVGSLNVIEKLQNNEDEHVKNAGDLLFEKTQ
                     NNILKLVFSDGTNPALNIQEKATILQVKGLDMPKADDDTSSYSTSEKNGITLMLLIGK
                     FLEKFGSRRDVQTTIFIDEGWAFSASRQGKKVGKRIKRTGRSENNSLVFITQSVKDKA
                     DDDGGNFGCHFAFDEKDEREDILKSLGLEYSKESPENMEMLKDLKKGQCIFSDFYGRV
                     GKMVVHCPFEEMTEAFRTQEDSASSKAEEKFAM"
     gene            complement(7661..8044)
                     /locus_tag="O0394_001193"
     CDS             complement(7661..8044)
                     /locus_tag="O0394_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCY0499281.1"
                     /translation="MDKKAYNLKRTFEQPIVMYEFTDKFRINKGFRLDFWATFLIVWF
                     ILFLLFWYLLQPVIMSIGGLMFIYFTVAPYYITKYIVKLKQDGKKLFFFLWDFVIFVF
                     NVQLRKVKLSYDEEVEYHDKKITFK"
     gene            complement(8056..8316)
                     /locus_tag="O0394_001194"
     CDS             complement(8056..8316)
                     /locus_tag="O0394_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499282.1"
                     /translation="MSGLFNFFVLGADRPSLGGVGDWISNEVGTGIGLVVLVVGIVKW
                     ASGKYGHMVILFVVGGFLFLVSKGPEQVFNAIAGVWKMIFGG"
     gene            complement(8321..9376)
                     /locus_tag="O0394_001195"
     CDS             complement(8321..9376)
                     /locus_tag="O0394_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCY0499283.1"
                     /translation="MKIISKYILTRFSKNGKHFDIPKEQRRVIPKFKNRRKMIVICFY
                     SLLAILLILLLASFIKATRANNDSKEAVNKTNMIQKKYEDNANTVQYSPKLKLYADKF
                     IDTYMNISKDSKELESREKELLKYFPSDYKKPDEKISDTERKLNSKEFYNIKRKDKQT
                     IIQYIVNYDVNITEKKEVKVKKKKKSEKDKDEYETKTEEKQRKVNQNILINIPIKSEN
                     NKYVVVEYPYFTPIPDSQLNQAKMVEDNLKDNKREDNPKAKAFIEDFFNKYASSKSDD
                     MAYLMDNPEGLEGTREVSQIREIRLYPKGDDYVAKVEILMKDKDSPLENLEHYTLDIT
                     KKDGKYYVKNMTNSIGG"
     gene            complement(9437..10528)
                     /locus_tag="O0394_001196"
     CDS             complement(9437..10528)
                     /locus_tag="O0394_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCY0499284.1"
                     /translation="MTLKNQCTPLTNRGVERTNKSAVEAVVDWVQVTFHIAPISAVIE
                     DVIGLPITLFKKRNSGIYFYNRGYEFSNIKLYYSSDDESMGIHLQLTGTGCREFEHHL
                     QQLNKTWQDFFDKCLSVNANFTRIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRAGSA
                     INGFNLSDGRSKGATFYIGSKQSNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMR
                     KAYAVNCAKVLSRTDNISEIVKSILHNNLRFISPPKGGNDKNRKRWPLYRPWALFIKD
                     TEKLNLTTRPTLKSIEDNLDWLCKQVATTLDTVLTAESMAQSEGLLTDTDFLDKILAH
                     SQFNDEHTNRINHYLEALKQKKHLSKDKC"
     gene            complement(10703..11005)
                     /locus_tag="O0394_001197"
     CDS             complement(10703..11005)
                     /locus_tag="O0394_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499285.1"
                     /translation="MDYYTADRLYRYTNSSNLSEPILNYVASRINWGDKVSLMTLAKE
                     IQSKFNDSYVKENTVKGRPKIYADLCLLCMSLSEAGHGRMLQVNLEDCIYIGDIDV"
     gene            complement(11019..11339)
                     /locus_tag="O0394_001198"
     CDS             complement(11019..11339)
                     /locus_tag="O0394_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HCY0499286.1"
                     /translation="MKPVLDIKKTLGQLNFLGVDEKYKYENGERTDKTVYAYKLASQE
                     QGEQITVKTPNKVELDYLQECELVEPDVKMYVQMSGDFGTIAYSWNAEDIKVVGSNSA
                     LKQK"
     gene            complement(11490..11774)
                     /locus_tag="O0394_001199"
     CDS             complement(11490..11774)
                     /locus_tag="O0394_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499287.1"
                     /translation="MTEFTPFFDGSNQLNYLKFVILRFHGVSKRKICKMYNFAYFRIL
                     EVCEMAKKNDYRFTYKDYKFLKQYGVSNTFICKMYHISIEDLEFFEVMNR"
     gene            complement(11783..12211)
                     /locus_tag="O0394_001200"
                     /pseudo
     CDS             complement(11783..12211)
                     /locus_tag="O0394_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(12406..12630)
                     /locus_tag="O0394_001201"
     CDS             complement(12406..12630)
                     /locus_tag="O0394_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HCY0499288.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVARDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(12647..12850)
                     /locus_tag="O0394_001202"
     CDS             complement(12647..12850)
                     /locus_tag="O0394_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HCY0499289.1"
                     /translation="MSKINHITIVLSFTKVDANGKQTEFKRRFANINPDASNDQIKTF
                     SKLIERLTGETYSNIELIKSLSI"
     gene            13009..13569
                     /locus_tag="O0394_001203"
     CDS             13009..13569
                     /locus_tag="O0394_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCY0499290.1"
                     /translation="MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSEL
                     LTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            13588..15990
                     /gene="mnhA2"
                     /locus_tag="O0394_001204"
     CDS             13588..15990
                     /gene="mnhA2"
                     /locus_tag="O0394_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HCY0499291.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTMLMYIFWELTSVSSFLLISYWYNNGDSQ
                     FGAIQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHGLFIPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYVYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVLALRAV
                     SGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     SYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            15977..16402
                     /gene="mnhB2"
                     /locus_tag="O0394_001205"
     CDS             15977..16402
                     /gene="mnhB2"
                     /locus_tag="O0394_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HCY0499292.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWI
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            16399..16743
                     /gene="mnhC2"
                     /locus_tag="O0394_001206"
     CDS             16399..16743
                     /gene="mnhC2"
                     /locus_tag="O0394_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HCY0499293.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            16733..18229
                     /gene="mnhD2"
                     /locus_tag="O0394_001207"
     CDS             16733..18229
                     /gene="mnhD2"
                     /locus_tag="O0394_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HCY0499294.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            18230..>18462
                     /gene="mnhE2"
                     /locus_tag="O0394_001208"
     CDS             18230..>18462
                     /gene="mnhE2"
                     /locus_tag="O0394_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HCY0499295.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIKFLGVYLYQLITSSISTI"
BASE COUNT         6079 a         3196 c         2454 g         6733 t
ORIGIN      
        1 tataaatctt ataataactt tttgaccgct caacattttt atacttcttt gtatttttca
       61 catcttgaat actttctttt tctaactttg taacttcatc aatttcttta tcatatttct
      121 gaccacatgc agataataat ataactaatc ctaaaattaa tacaaaccat cgcctcatga
      181 taaattcact cctttttacg taaattatat cattgctatc gtcttgaaac aaactaacgt
      241 cctagaggtt ttaaattgaa acgttttaaa ttatcaatac gccaaccttc agctgtacga
      301 actaaatcca attgtgcaga tgtttgacga tgttcaattt tatcatctcc atctgtttca
      361 atacgttcgt cgaactgaac aaaaacttta taactatctt ttttagggtc ataagtatca
      421 aaataaacat gaatatcatt aaccgttata tcatcatata aatcatattt atctttagaa
      481 tccttaaata atgtatcgta ataatccttt gtaactaaag gctttatttg tggtgctttt
      541 gatttaaaag aagaatcttt tttcatttca aacatctgtt taacaaaatt tgaagaaact
      601 tcacgtattt gtttttcttc atcagaaata actgtttttg attgtctctt tactaattta
      661 tttaactctc catttttatc tcttaatata cgttgttctt cctgcaaatc ttcaatttga
      721 ttttgtttca ttttaacttg ttgtttagga atctgataac caattaaaac agcacaaaat
      781 aatattactg ttacaagagt aaacataata attttttgct tcatttattt tcactcctta
      841 tcatgaataa aactatatcc gtcagtcatt ttcaatgtac gttcattaac tttaaaagca
      901 ccttcccaat tcatttctgt tattctaaat gttttaccac catcacttac accaataaca
      961 acagccacat gaccataatg cccatcatga ccgcctgcac cttgttcagc aataaataat
     1021 gcaccaggtt ccggattttt atcaacttta taaccctcag ctttagctct atcattccat
     1081 ttatgtgcat catgccaata agtcgataca ggtttaccca ttttttgacg cataccgtat
     1141 gcataccaag tacattgacc agcagtgtat aaattacctt ttccagggtc atcatttgac
     1201 caatgtggca atttaccatt aaactttata gactttcctt taccattact acatgacgtt
     1261 ttagcttctg aaccgcctaa atctttcata attttttcaa ctgttggtat ccaacgggca
     1321 ttcatattat tagggtcatt tgacgcacca acaggcgcat atttgggacc tattttttta
     1381 ggagtatcta aacctttaga aatataaaca tcatacaaat ttttagctcc cgcattaaga
     1441 ccatcttcaa tagttggata tgttgaatca tgaatcgatt ttgaacccat aacagacaaa
     1501 ggattatttt gtcttgtcgc attttctcct ttaccccatt gtgattcact cgcaataatg
     1561 gccataaaaa tattcacagg tactttattt ttttctgcaa ttttctgtat ttcttttcct
     1621 ttaccctcta attttccacc tttagcattt tcgttaaact tatcaatacc atttgccgaa
     1681 acattaccgc cactaacagt acaaccagca tcatctttac ctttatttaa atcaggcgct
     1741 aacaaaaaca ttataattac tggaaatata aaaaaagcca ccacacataa aatgatgatg
     1801 actttcgcta atttcaattt aacgcctatt ggcgttttac ctattacttt tttagcaata
     1861 tcttttttat ttaccatgct tattgatatc cttttgctct tttaaacggt cttttgactg
     1921 agaattacgc gtaataggtt gattacgatt tccagattgt ctactttgtt gattctgtgt
     1981 cgcattttgt atttgtcgtt tttgaacatc agtatcataa gcagtttgaa ctgacttctg
     2041 ttctttacga tttattgaat gattctcatt atttaatggc tgattaacca cttgtggcga
     2101 cctttgtata tcttttcgct gtcttgtttc tcgtacttga tgttcttgct gtgatgcttt
     2161 ttgtggttgt ttagaataat tatttgcatt tgattcaaca gaagcagatt ttacatcgtt
     2221 accattcatt gactcttgtt gttctctgcg cttattacca tttttttgat tatctttatt
     2281 tataaataaa ggcattggtt tatcctgttg tcttcttctt aaatcattat cattttgaag
     2341 tggtggggcg ggttcatctc tttgattttc tcgtttagct ttcttatttt gcgcttgttc
     2401 atgacgaatc ttattaaaac gttcttgtgt tcgattcatc acaccagcac caattgcact
     2461 aggagaaaaa ctaatattac cacgacttac aaaattgata atttgagccc gttttttata
     2521 cattacaaac aatattacaa ttataaatat tgaatttaaa aaataagcat tttgactatc
     2581 cggttcaata aaactatttg caagcttgat acacaaacca actatcacaa ataagaatgt
     2641 catacaagca cttaaaataa tatagcctaa cgtttttgca aacgcagaga aaacagataa
     2701 ttcaaatcct ggtataaaac tcactatcca actgaatact aaaaataaga ataaaaataa
     2761 agcaaatgta gaaaaaatca acttcgcaaa acttattgat aaaaatacta tcgacataaa
     2821 aagtgtaata ataaaaccaa tcactgaaat agccaacttt tgaaatacat aaccttgcgt
     2881 catatattta ttatttttct tttcaacctc atctttaaca atatcatcta attctttttc
     2941 tgatttttta tcataatcaa gttttaaaat gttatcaata cggtctttac cttttgcttt
     3001 tatctctttt tcgtcgacag ttccataatt cattactaaa tatgttctat atatcgtcat
     3061 atcaaataat tgattacgta tatgattcat acctgaattc tcatgaatat aatcaatatt
     3121 accatcaata gaagatgtac tattcataat tatattattt aagtccttac caatattgtt
     3181 tgtaccacgt ataatattac ctgcattcgc aagtatacca acaccaaaac caattaaaca
     3241 cataattaaa aacgctcgtt ttaatgcttc tcttgcatta ccttgtacac taaaaataat
     3301 aaatgcgtta aaacaaacaa taataaataa tgttaatgct aaattactca ataacttttg
     3361 atatatatcg ccaacaaaat cgccgatttt atccgcaaaa tcattaataa catctaattg
     3421 aaataaattt tcaatcgcat aatctgtaac acctgctaaa atcttagtca tagaaaagaa
     3481 aaaatctgac acattattaa tttgacgtga aatttcttct tgaccaaacg gattccagtc
     3541 gcctttttca ctatatattg aacgataatg tgataaatca tacttttcaa tttttggctt
     3601 aactgcatgt gagggctctc caactggatt agaaacagca taagcactca cagatgttaa
     3661 aagcagaata ggcaaaatta aaaagcataa aagatacaca cttttatgct tcatcaaatc
     3721 actccttatt taatatttgt ttcacatctt ccacaaaatc atattgttcc attaaaggtg
     3781 catcaaataa aattggttca ttatattgtc caagtataga aatataacct cggccgatat
     3841 cactatcatg aatcgtttga aattctttat cagtttgacc aaaggtcata cgataaccat
     3901 catttgacaa attacctaaa gacacacgta accctaattg gtcacgcaca ttacctttaa
     3961 tcacatctgc atctggtctt tgtgctgtta aaaacataaa tacaccagct tgacgacctt
     4021 tcataataat attaattaaa taatcattca tttcttgtaa ttctttttta tctaaagttg
     4081 aagtaaaagc tgttacctca tcaaaaataa tgaaataggg gcgcatatca aaattacgga
     4141 aatcattccc gattttataa tccgaactat cattcattaa tctaaaacgc tcttccattt
     4201 cattattagc ttctctcaat tgttttaata tttgaccttt agtatctgca actttatcat
     4261 caccaataac gcgcttcata aagctcaaat ctgaaacttt tgggtctaat aaacgtactt
     4321 ctgaatgtct acggaataac tcacgaatca cataaaataa aaaatatgtt ttaccacctc
     4381 cggttatacc tgtaaccaat ccatgaggtg ctttcacaaa atcccaagta atttgatgcg
     4441 taatagcaat ttttgtatca taaaaaattg tatttgattc ttgttcgcca ttcataacaa
     4501 tacgcttttt atatttaagt ggcataaatt catatctcaa ataacgacca atatgctttt
     4561 tttctactaa ttcagaatta aacattggtt caataatcgt ctctaactct aaataattat
     4621 cttgaaacct cgaaccgtct ttcttaatat gaataacaaa agatttttta ccacgcttat
     4681 aaaacattgt tggataataa tcatattcag tttgtttata atcaataggg atatactcag
     4741 ttgttttctt gattactgta cgattatgaa aaccttgtgt ttcaatcatt tcagccaaat
     4801 cttttgtttt aaaacgtatt aaaaactgag caataagctt acatatcaca aatataattg
     4861 ctgtaccaat taataaatac aataaatgcg gtttaaaata tgcaaacaac gtcaatatat
     4921 ccaatttttt attaactgat ttataatcaa taaacaaaat atatatacga tataccaaaa
     4981 aaataaatgc taatacactt gcaaacataa aatcaatata cttctgataa ggctgtatac
     5041 gtacgccttt aaataaagca atttcattca ttgccatcaa atccactcct ttaaaataaa
     5101 aagcacatac aattttgtat gtgctaaaaa ttacatcgca aatttttctt cagctttaga
     5161 gcttgcagaa tcttcttgtg ttctaaatgc ttcagtcatt tcctcaaatg gacaatgtac
     5221 aaccattttt ccaacacgtc cataaaaatc actgaatata cattgacctt tcttcaaatc
     5281 tttcaacatc tccatgtttt caggagattc ttttgaatat tctaagccta aagattttaa
     5341 aatatcctca cgttcatctt tttcatcaaa tgcaaaatga caaccaaagt taccaccatc
     5401 atcatctgct ttatctttta ccgattgtgt aataaataca agtgaattat tctcagaacg
     5461 tcccgttctt tttatacgct taccaacttt tttaccttgt cttgaagcac taaaagccca
     5521 accctcatca ataaatatag tcgtttgaac atctctacga gaaccgaatt tctctaaaaa
     5581 cttaccaatt aataacatta atgtaatccc atttttctca cttgttgaat aacttgatgt
     5641 atcatcatct gctttaggca tatctaaacc tttaacctgt aaaatagttg ctttttcttg
     5701 aatatttaaa gctggattcg taccatcact aaaaacaagt tttaaaatat tgttttgcgt
     5761 tttttcaaat aataaatcgc ctgcattttt tacatgctca tcttcattat tctgtaattt
     5821 ttcaattaca tttaatgaac caactttttt accattttca cgttcttcaa ccgtatcaga
     5881 aatagcttga taaattgctg tttcaatatg atgataattt tcaaaagatt taatttcatc
     5941 aaacactgat ttcaacatct cattagcttc aataccatct aaaaatacaa tagggtctag
     6001 acaaccataa ttggttgaat cttcataatt caatgttaca tatttaaatg tttttaaatg
     6061 attaataaat aggggatact tttcttgcat ttccttactt tctgttactc tgtcaaacca
     6121 ttctgcaaac tcagattttg gatcccagta caacatttga cctttaaaaa atgatgtata
     6181 aatccataat aattttgcta aaaatgattt acctttccca gtttgaccag taattaaaac
     6241 atgcggacta tcactgacag cccccttaat accttttgcg gccaacaata aactaaacaa
     6301 aattatatcc cttgaacttg caacagcttc ctcacgtgaa acagaacgta taaatttatc
     6361 aatacgccct atataaaaac ccgtttttgt tcccaaactt tgagacacac caaataaatt
     6421 ctctgctata cctgttgccg ttgttctttg taaccaatac ttattaataa ataaatcatt
     6481 accatgtaaa cattgataaa acaattgtaa ctggtcagct aatggattaa ctgcatatat
     6541 ttgagtttcc tttaatcgtt tcatcaaacg ctttgcttta cgtttacaat ctctataatc
     6601 atcactttta attacaaaca taaataccca ctcaataaat acacctgagc cagtcacttc
     6661 tcgttccatt tgattcaata caaacaaatt atcttgaaca tcttccgaag cactgtcgcc
     6721 agtatcttgc ttttcttttg cttgttgttt atgatttatt ttcgcacggt caaatttttt
     6781 cttaactttc tcattattaa tgatttttgc ttttacatgg cattcaaccg gaaaaggcat
     6841 attttgagct ttctcaaata aatgtgtata ttccatatct aacggaaaat catcaacaac
     6901 aactgttgtt gtataaaatt tctcgttatc attttcaact tctaaaacac tcaagcgagt
     6961 agggtcaatt actgcattat aaatatctgt aatagctctt cttgaagatt cttcctgtac
     7021 gtcatgtgtt gcatttgaaa taaaaggctt tctaataata taagccaatt cattctcagt
     7081 taaacgctcg cctccaatac ctagaaaact atcagccaaa tctcgttcaa atgttttata
     7141 cttatctaaa attttatcgt ctaaattcac atcaaaacca aaattcgaaa caatacgctc
     7201 agccgtatct gataatgtac tttttaaaat ctctttaagt gtaactgcat cgtcagctaa
     7261 ttcacgtatt ttaacaccca taacaaaaga attatgcgta atcatttgta attcttgttc
     7321 taatacgtca atagtttctt ttgaataata ttcagctaca ttaaaagctt catcatcaaa
     7381 atctttacta atttctttaa atttatttcg caaatctaaa tcgtggtcat acatttcaaa
     7441 atgaaaatct tgataacctt taatcgactc taaaaaataa cgcatacgct tttttgattc
     7501 ttcttttgca tcataatttg ctgttgatat agactcagaa cgaacacgat aatacgccca
     7561 aacttcgcca gttttcgtta ataacaaatt atctcttaaa cctgcatacg gtgcttttaa
     7621 attcatgtca tccctccaat aaaaataacc ccaatagggg ctatttaaat gttatttttt
     7681 tatcatgata ttctacttct tcatcataac ttaatttaac ttttcttaac tgcacattaa
     7741 aaacaaatat tacaaaatcc cataagaaga aaaataattt ctttccatct tgttttaact
     7801 taacaatata tttcgttata taataaggtg caaccgtaaa gtaaataaac ataagtccac
     7861 caatcgacat aattacaggc tgtaataaat accaaaataa caaaaataat ataaaccaca
     7921 caatcaaaaa tgtagcccaa aaatctaaac gaaaaccctt attaatacga aacttatctg
     7981 taaattcata cataactatc ggttgttcaa aagttcgctt taaattataa gcttttttat
     8041 ccaaatggaa tcacctcaac cgccaaaaat cattttccaa acacctgcaa tcgcattaaa
     8101 tacctgttca ggacctttag aaactaagaa taaaaagcca ccaacaacaa ataatataac
     8161 catatgccca tatttaccac tagcccattt tacaatacca acaaccaata ctactaaacc
     8221 aatacccgta cctacttcat tacttatcca gtcaccaact ccaccaagtg atggacggtc
     8281 agcacctaaa acaaaaaaat taaataaacc actcataaca ttaacctcca atagaattag
     8341 tcatattttt aacgtaatat ttaccatctt tttttgttat atctaatgtg taatgctcca
     8401 aattctccaa aggagaatct ttatctttca tcaaaatttc aactttagca acataatcat
     8461 cgccttttgg atataaccta atttctctta tctgagaaac ttctctagtt ccttctaagc
     8521 cctcagggtt atccattaaa taggccatat catcagactt actagaagca tatttattaa
     8581 agaaatcttc aataaaagct tttgctttcg gattgtcttc ccgtttattg tctttcaaat
     8641 tatcctcaac cattttagct tgattcaatt gactatcagg gattggcgta aaataaggat
     8701 attcaacaac aacatactta ttattttcac tcttaatagg tatatttatt aaaatgtttt
     8761 gatttacttt acgttgcttt tcttctgttt ttgtttcata ctcatcttta tctttttcac
     8821 ttttcttttt cttcttaact ttaacttctt tcttttcagt aatattcaca tcataattaa
     8881 caatatactg tataattgtt tgtttatctt tacgtttaat attataaaat tctttactat
     8941 tcaattttcg ttctgtatct gaaatttttt catcaggttt tttataatct gatggaaaat
     9001 atttcaacaa ttctttttct ctagattcca attcttttga atctttcgag atattcatat
     9061 acgtatcaat aaacttatca gcatataact ttaatttagg actatactga actgtattcg
     9121 cattatcttc atactttttt tgaatcatat tcgttttatt aactgcttct ttactgtcat
     9181 tatttgctct tgtagcttta ataaaagaag ctaataaaag aattaacaaa atagctaata
     9241 atgagtaaaa acaaattaca atcatcttac gacgattttt aaattttgga attactcggc
     9301 gttgttcttt aggaatatca aaatgctttc cgtttttaga aaatcgtgtt aaaatgtact
     9361 tgcttattat tttcataaac gattttctaa aatcctttac gaaattttta ataaaaatca
     9421 catcccagtt atttttttaa cacttatctt ttgataagtg ctttttttgt tttaaagcct
     9481 ccaaataatg attaattcta ttcgtatgct catcattaaa ttgtgaatgc gctaaaattt
     9541 tatctaaaaa atcagtatct gtaagcaacc cctcagattg cgccatactt tcggcagtta
     9601 acactgtatc taaagtagta gcaacttgtt tacataacca atcaagatta tcctcaatgg
     9661 actttaatgt tggtctagta gttaaattca atttttcagt atctttaata aataacgccc
     9721 aaggtctata taatggccac cgcttacgat ttttatcatt tccaccttta ggaggagaga
     9781 taaaacgcaa attattatgc aatatagact ttacaatttc gcttatatta tctgttctac
     9841 tcaacacttt cgcacaatta actgcatatg cctttcgcat ttgtatttca taacgattcc
     9901 atagcccaat gtcttcgaca tcacaatgac gtttaaacgc ttgttcataa ttcttttcat
     9961 aaaaacgaca atataaattg ctttgtttag aaccaatata aaaagtagcg cctttacttc
    10021 ttccgtctga caaattaaaa ccgtttattg cgcttccagc tctaaaacgt gaaacacact
    10081 cagctttttc agccttttta attaataaag gcacttttaa atatgtttta taatcatcaa
    10141 ttgcaatatc aattcttgta aaatttgcat taactgataa acatttgtca aaaaaatctt
    10201 gccaagtttt attcaactgc tgtaaatgat gttcaaactc tctacaacct gtaccagtta
    10261 actgtaagtg aatgcccatt gattcatcat cacttgaata atacaattta atgtttgaaa
    10321 attcataacc gcgattataa aagtaaatgc cactatttcg ttttttgaac aacgttatag
    10381 gcaaaccgat aacgtcctca attactgctg aaataggggc tatatggaaa gtgacctgaa
    10441 cccagtcaac aactgcctcc acagcacttt tatttgtacg ctctaccccc ctattagtaa
    10501 ggggggtaca ctggtttttt aaagtcatat ttatttatgc ctcctatcaa atcgcaagcg
    10561 atttgataca tgaccgttct cgctttttta acgcgtattt gtcgcaggct ccgcccaaat
    10621 gcttaacgca ctccgtgctt gcatttctgc gcctgcatac gctaaaagct acacggtcat
    10681 gttaaatcac taatactata tgttaaacat caatatcgcc aatataaata caatcttcca
    10741 aattcacttg tagcattctg ccatgtcctg cttcactcaa gctcatacac aatagacaaa
    10801 ggtcagcgta aattttaggc cgacctttta ccgtattttc tttaacgtaa ctatcattaa
    10861 atttagactg tatttcttta gctaaagtca ttaatgaaac tttatcgccc caatttatac
    10921 gactagcaac atagtttaaa ataggttcac tcaaatttga actatttgtg tagcgatata
    10981 atctatctgc tgtataataa tccatttaca tcacctaatt atttttgttt aagtgcacta
    11041 ttagaaccaa ctactttaat atcttctgca ttccagctat atgcgatagt tccaaaatcg
    11101 cctgacattt gaacatacat tttaacgtct ggttcaacta attcgcattc ttgtaaatag
    11161 tctaattcca ctttattagg cgttttaaca gtgatttgtt cgccttgttc ttgactcgct
    11221 aatttatatg cataaacagt tttatcagta cgctctccat tttcatattt atatttttca
    11281 tctacaccta aaaagtttaa ttgtcctaaa gtttttttaa tatccaatac tggtttcata
    11341 atatatccat cctttacaaa atttttaata aaattaaacg acttctaaag ggtcgcacga
    11401 ataggaataa ctcccgcaaa tccgtaccac tgcatttttg ataatggagt gtctatgctt
    11461 ctcctgacct atcatatttt taatcaaacc tatcggttca tcacctcaaa aaattctaag
    11521 tcttcaattg aaatatgata catcttacaa ataaatgtat tagacacacc atattgtttt
    11581 aaaaatttat aatccttata agtaaatcga taatcattct tttttgccat ttcacaaact
    11641 tctaatatac gaaaatatgc aaaattatac attttacaaa tttttctttt agaaacacca
    11701 tgaaatctta aaattacaaa tttaagataa tttaattgat ttgaaccatc aaaaaaaggt
    11761 gtaaactcag tcataattat caattccttt cataagaatt aactgttaac taaagtttac
    11821 accaccaatt aaaatcatga attgtagcgc caaataatcc aaatgtatca ataatgacac
    11881 ccataataat ttgacccgca actgttgcaa ttacagttaa tgttgcacct aattttggca
    11941 ataacaataa attgccagtt aaaaagctaa ccccaagcaa accaccgact acccatgtgt
    12001 agttaaatga ttgattattg taaaagtgaa tagtaaatac ttctggattg ataatgatat
    12061 ttaaaataat taaacaaatt gttccaactg aaaatgaaat gaatgaagta tagaaaggcg
    12121 atttagtata cagtgatagt cttgaattga cagatgtttg gataggaata agcattccaa
    12181 ctaggacacc aatgatatag aaaagaagca tatgaataaa gtctcacttt ccaaagttaa
    12241 taataaaatc atatatttac attatcatta tagagaaaat atacaactca tttacaataa
    12301 tgttgaaaaa aacaggacat tattacaaca aaatcaccct agacaatcat gatgaaagtc
    12361 tagggtgtta gtatttatac taaattaatt gcgctttctt tatatctatt tcttatcttc
    12421 aaataaaaca gttgttgttt tatcaataat ttgagcagaa tgtactttgg tgatagtacc
    12481 actatttgac tttataatat ttaaatctac taatgcattc atactatcac gtgcaacttg
    12541 ctcggtcact acgttgttta attgtggtaa ttgcagtttt acaggcttgt ctagtgaaga
    12601 tttaaaactt aattcaagtg ttttagtcat gaatatgtcc tcctgattaa attgataaag
    12661 atttgatgag ttcgatattg ctatatgttt ctccagtaag tcgctcaata agtttgctga
    12721 atgttttaat ttggtcgttt gatgcatcag ggttaatgtt agcgaatcga cgcttaaatt
    12781 ctgtttgttt gccgttagcg tctactttag taaatgataa tacaatagtg atgtggttta
    12841 ttttactcat attttaaaac ctcctttcac actatatatc gaaacaaaat aataaaatgg
    12901 ctaattttat tttctatgtt taaaatctat aaaaaaggca atagatatat gtaactaaaa
    12961 tataatatag ctatgattaa gacacttaaa gcaaggggag gttgagtaat gaataaagta
    13021 gaagcgatta aatttaatga tgatattgtt aaaatgtatg aagcgctcaa gataaaatct
    13081 gaacgtgact atttattctt taagttagct atacatagtg gattgaaagt atcagaatta
    13141 ttaacaatta cagtctctca agttaagaga ctaattgaaa agtgtacgtt atcagaaatg
    13201 tgtaaagcac attttcattc gttgattaaa attaggttac cagaaacatt atcgaaagaa
    13261 ctacttcaat atatagagga caggagtctt tcgaatgaag acgttctttt tcaatcacta
    13321 cgaacaaatc aagtattatc tagacagcaa gcatatcgaa taattcacca agcatcaatt
    13381 gaagctggta tagataatgt aggactaacg acattgcgta agacatttgc atatcatgct
    13441 tatcaaaaag gtatacctat accagtcatt caaaagtatt tagggcatca atctgctatt
    13501 gaaacactaa attttatcgg tttagaaaat gagtgtgaac atagtattta tatttcatta
    13561 caattataga aacaaaggag gctaataatg agtttggttt atttattaat tgctatactt
    13621 gtgattatgg cgatgatact tctaatgtct aaacgtagag cattggctaa atatgccggg
    13681 tacatagcgt tggttgcacc tgtaatttca tctatctatt ttttgattca aataccatca
    13741 gtagctaaac tgcaatatct ttctacctct attccatgga ttaagacatt agatattaat
    13801 ttagatttac gtttagatgg tttaagttta atgttttctc ttattatttc acttattgga
    13861 attgcagtat tcttctatgc aactcaatat ttatcctctc gaaaagacaa tttaccaagg
    13921 ttttattttt atttaacgtt atttatgttc agtatgattg gtattgtatt atcagacaat
    13981 acgatgttga tgtacatttt ttgggaatta acgagtgtat catcattttt attgatttca
    14041 tattggtata acaatggtga cagtcaattt ggtgcgattc aatcatttat gattacagta
    14101 tttggtggat tggcgttatt agttggtttt attatgctgt atatcatgac aggaacgaat
    14161 aacatcacag agatattagg acaagcagat catattaaga atcatggatt gtttatccct
    14221 atgattttta tgtttttatt aggtgcattt acaaaatcag cacaatttcc atttcatatt
    14281 tggctaccta gagcaatggc tgcacctaca cctgtaagtg cttatttaca ttcagccacg
    14341 atggtaaaag ctggtatctt tttattactt cgatttacac cattattagg tcttagcaat
    14401 atgtacgtat atatcgttac gtttgttggt ttaataacaa tgttatttgg ttcaattaca
    14461 gctttaaaac aatgggattt aaaaggtatc ctagcgtact ctacaatcag tcaacttggg
    14521 atgattatgg ctatggtggg tataggtggc ggatatgctc aacaccaaca agacgcaata
    14581 gcatctattt atgtatttgt attatttggt gcgctatttc atctaatgaa tcatgccatc
    14641 tttaaatgtg cgcttttcat gggagtaggt attttagatc atgaagcagg ttcaagggat
    14701 atacgaattt taagtggaat gcgtcaacta tttcctaaaa tgaatctagt catgacgata
    14761 gcggctctat ctatggctgg agtaccattt ttaaatggat ttttaagtaa agaaatgttt
    14821 ttagatgcat taacacaaac tggacaatta tcccaattta gtttgatttc aatgatagct
    14881 atcgtgtttg ttggtgttat tgcgagtgtt tttacattca catatgcact atacatggta
    14941 aaagaagtat tttggacaaa atatgattct aaggttttta ctaaaaaaaa tatccacgaa
    15001 ccatggttgt ttagtttacc atctcttata ttaatggtgc tagtacctgt aatctttttt
    15061 gtaccaaata tatttgggaa ggggattatc gttctagcat taagagctgt atcaggtggt
    15121 aatcatcaaa ttgatcaatt ggcaccacat gtttcgcaat ggcatggatt taacataccg
    15181 cttcttttaa ccatcatcat tattttattg ggtagtgtac tagcaatcaa agtagattgg
    15241 aaaaaagtgt tcacaggtaa aattagacag atttcagttt caaaaagcta tgagatggta
    15301 tatcgacatt ttgaaaagtt tgctacgaag cgatttaaac gtgttatgca agatcgttta
    15361 aaccaataca ttattatgac cttaggcata tttatgatta tcattggata tggttatatt
    15421 cgaattggac ttcctaaagt acatcagtta catgtttctg aatttggggc attagaaatt
    15481 atattagcaa tcgtaactgt cacaattggt atttctttaa tttttatacg tcaacgactg
    15541 acaatggtca ttttaaatgg agtcatcgga tttgttgtga ccttattctt tatagcaatg
    15601 aaagcccctg atctagcatt gactcagcta gtagttgaaa caataacgac gatactattt
    15661 attgtcagtt tttcaagatt accaaacgtg ccaagatcta acgctaacaa aaaaagagaa
    15721 ataattaaaa tttctgtatc actcttgatg gcacttattg ttgtatcatt aatttttatt
    15781 acacaacaaa cagatggttt atcatcaata tcagactttt atttaaaagc tgacaaacta
    15841 acaggtggta aaaatattgt aaatgcgata cttggtgact ttagagcatt agatacatta
    15901 tttgaaggat tagtgttaat tattactggg ctaggtattt acacattatt aaattatcaa
    15961 gatcggaggg gacaagatga aagagaatga tgtcgtgtta agaacggtca cgaaacttgt
    16021 tgtatttatt ttattgactt tcggattcta tgtcttcttc gcaggtcata ataatcctgg
    16081 tggtgggttt attggtggtt taatatttag ttcagcgttt attttaatgt ttctggcttt
    16141 taatgttgaa gaggttttag aaagtttacc gattgatttt agaattttaa tgattattgg
    16201 agcattggta tcatctatta ctgcgataat acctatgttt tttggaaaac catttttgtc
    16261 tcaatatgaa acaacttgga tacttccaat tttaggacaa attcatgtaa gtacaataac
    16321 actttttgaa ttaggtattt tattctcagt tgttggtgtt attgtcacag tgatgttgtc
    16381 gcttagcgga ggtcgatcat gaatttaata ttattactag ttataggatt tttagtgttt
    16441 ataggaacat atatgatttt atcaatcaat ttaattcgta ttgtaatcgg aatttcaata
    16501 tatactcatg ctggtaatct cattattatg agtatgggaa cgtatggttc tagtagatca
    16561 gaaccactaa taactggtgg aaaccaattg tttgttgatc ccttgttaca agctattgta
    16621 ctaactgcaa tagttatagg gtttgggatg actgcgtttt tacttgtact tgtttataga
    16681 acttataaag taacaaaaga agatgaaatt gaaggcctaa ggggggaaga tgatgctaag
    16741 taacttattg attttaccaa tgttattacc attcctttgt gccttaatcc ttgtattttt
    16801 aaaaaataat gatcgtattt ctaaatattt atacttaggt acaatgacta tcaccacaat
    16861 tatttcatta atgctattaa tttatgttca gcgtcaccgt ccaattacgc tagactttgg
    16921 aggatggtca gcgccctttg gtatacagtt tttaggagat tctttaagtt taattatggt
    16981 tacaaccgct tcgtttgtga ttactttaat tatggcatac ggatttgggc gtggcgaaca
    17041 taaagcaaat cgttatcact tgccatcgtt catattattt ttaagtgttg gcgtgatagg
    17101 ctcttttcta acatcagatt tatttaattt atacgtcatg tttgaaatta tgttactagc
    17161 gtcatttgta ctcattacac ttggacaatc tgtagaacaa ttacgtgctg caattattta
    17221 tgttgtcttg aatattattg gttcatggct attcttatta ggtataggtt tactttataa
    17281 aacagtaggt acattaaact tttcacatat tgcaatgcgt ttgaatgaca tgggagataa
    17341 tcgcactgtt acaatgattt cattaatctt cttagtcgca tttagtgcga aagcagcgct
    17401 ggtccttttt atgtggctac ccaaagccta cgctgtgtta aatactgagc ttgcagcatt
    17461 atttgcagcg ttaatgacca aagtaggggc ctatgcatta attcgattct tcactttact
    17521 atttgatcaa cataatgatc tcatacatcc attgctagca actatggctg ctataactat
    17581 ggtcatcggc gctataggtg tcattgctta taaagatatt aaaaagattg cagcttacca
    17641 agtcataatc tcaataggat ttatcatttt aggtttagga acaaacacgt ttgcaggtat
    17701 taatggtgca atattttatt tggtaaatga cattgttgta aaaacattgc tattttttat
    17761 tattggtagt ttagtttaca ttacaggcta tcgacaatat caatatttga atggcttagc
    17821 taaaaaagaa cctttatttg gagttgcgtt tattataatg atttttgcta ttggcggcgt
    17881 gcctccattt agtggctttc cggggaaagt acttattttc caaggtgcat tgcaaaatgg
    17941 caattatatt ggactagcgt taatgattat tactagtcta attgcaatgt acagtttatt
    18001 taggatactt ttttatatgt attttggaga taaagatggg gaggaagtta attttaagaa
    18061 aatcccgcta tatcgaaaaa gaattttaag tattttagta gttgtggtta tcgcaatcgg
    18121 aattgctgca cctgttgtgt taaatgttac aagtgatgca actgagttga acacgagtga
    18181 tcaattatat caaaaacttg taaatccgca tttgaaagga gaggactaaa tgaatcaaat
    18241 agttttaaat attatcattg cattcttatg ggtattattt caagatgaag atcattttaa
    18301 attctcgact ttcttttctg gatatctaat tggtttaatt gtcatttata tattacacag
    18361 gtttttcagc gatgattttt atgttagaaa aatatgggta gctattaaat ttttaggtgt
    18421 ttatttatat caattaataa catctagcat tagcacgatt aa
//
LOCUS       DAKTLH010000042        18318 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.042, whole genome shotgun sequence.
ACCESSION   DAKTLH010000042 DAKTLH010000000
VERSION     DAKTLH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18318
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.042"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(113..379)
                     /locus_tag="O0394_001209"
     CDS             complement(113..379)
                     /locus_tag="O0394_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCY0499296.1"
                     /translation="MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
     gene            complement(379..636)
                     /locus_tag="O0394_001210"
     CDS             complement(379..636)
                     /locus_tag="O0394_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCY0499297.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            995..1450
                     /locus_tag="O0394_001211"
     CDS             995..1450
                     /locus_tag="O0394_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HCY0499298.1"
                     /translation="MKKLCSLIVVALVCIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            complement(1618..3195)
                     /locus_tag="O0394_001212"
     CDS             complement(1618..3195)
                     /locus_tag="O0394_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HCY0499299.1"
                     /translation="MTFLTVMQFIVNIIVVGFMLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            3390..4139
                     /locus_tag="O0394_001213"
     CDS             3390..4139
                     /locus_tag="O0394_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499300.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRLMHEDAFIPMIRSQIVASA
                     KYKWHILISFTLSVVLFLLLNHFNIFALNVDSFTVFDIYPLIKGYGSISLITIFSLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSIILETLFYYPYINTWWEFIPALILAG
                     SATYLYLKSYRNFMVSYFYQLAVILVLHIFM"
     gene            complement(4363..5556)
                     /locus_tag="O0394_001214"
     CDS             complement(4363..5556)
                     /locus_tag="O0394_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0499301.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPR
                     PASSL"
     gene            complement(5568..6317)
                     /locus_tag="O0394_001215"
     CDS             complement(5568..6317)
                     /locus_tag="O0394_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0499302.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKEVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            complement(6310..7125)
                     /locus_tag="O0394_001216"
     CDS             complement(6310..7125)
                     /locus_tag="O0394_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0499303.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNPERLGVTGEIIFDGTSMLSLSESQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEQGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            complement(7122..7991)
                     /locus_tag="O0394_001217"
     CDS             complement(7122..7991)
                     /locus_tag="O0394_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0499304.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            complement(7988..8923)
                     /locus_tag="O0394_001218"
     CDS             complement(7988..8923)
                     /locus_tag="O0394_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0499305.1"
                     /translation="MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            complement(8936..10534)
                     /gene="cntA"
                     /locus_tag="O0394_001219"
     CDS             complement(8936..10534)
                     /gene="cntA"
                     /locus_tag="O0394_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HCY0499306.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            complement(10677..11978)
                     /gene="cntM"
                     /locus_tag="O0394_001220"
     CDS             complement(10677..11978)
                     /gene="cntM"
                     /locus_tag="O0394_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HCY0499307.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKAKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFNKDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMVILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQHLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            complement(11971..12789)
                     /gene="cntL"
                     /locus_tag="O0394_001221"
     CDS             complement(11971..12789)
                     /gene="cntL"
                     /locus_tag="O0394_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HCY0499308.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            complement(12800..13621)
                     /gene="cntK"
                     /locus_tag="O0394_001222"
     CDS             complement(12800..13621)
                     /gene="cntK"
                     /locus_tag="O0394_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HCY0499309.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVKQVTTE
                     IQHLVEAFVREQQWSHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            complement(14488..14853)
                     /locus_tag="O0394_001223"
     CDS             complement(14488..14853)
                     /locus_tag="O0394_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499310.1"
                     /translation="MLKEKESFRLLYQAIREIADKIGDNQLETNSVSLLLLDFDFEHE
                     VFDELYLVILKYLNTVNIENISHSELLNLIENTIPEDREINTFVKNKIIIGFANNYFP
                     ELQVLANEIKSDMASSLKQ"
     gene            complement(15116..15934)
                     /locus_tag="O0394_001224"
                     /pseudo
     CDS             complement(15116..15934)
                     /locus_tag="O0394_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503368.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            16167..17705
                     /locus_tag="O0394_001225"
     CDS             16167..17705
                     /locus_tag="O0394_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCY0499311.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGKYDDSLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            complement(17817..18239)
                     /locus_tag="O0394_001226"
     CDS             complement(17817..18239)
                     /locus_tag="O0394_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCY0499312.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDRL"
BASE COUNT         5767 a         3535 c         2693 g         6323 t
ORIGIN      
        1 aaattaaaaa gtgtagagat aacaacgcat taacgtcgct attttcccta cacttaaata
       61 ctagtttctt taattaactt atcattaaag ttattcagat attgaatatt cattaatcat
      121 aatgtgacca tgccgatagt attaatactt ctttatttcg atcatctacg gtatagacga
      181 ccctgtgctg atggttaatt cttcttgaat atcgctctaa atatttaggc tctaattttt
      241 caaaagattg tgttatttta tacggatcat tttttaaagt ctcaacaatt tctaaaaatg
      301 acttctttaa ataagaatgt tttattttct ttaaatctga tttcgctgaa tttttaatct
      361 taaccgtgta attgctcatt aaagattatc ccaatcaata tcatctatat ttgttgtacc
      421 actattatct ttttctcttt ctcttacttt gtccattgta ccagtagatt caagatatat
      481 tgtctcttgt atacttttcc aatcttctaa acctataatc acagcattat tttcggcatt
      541 attgccacta atataaattg gttcgtgatt attattaaca ttttttagta attgataaaa
      601 atcttttctc gcttctgtag ggctagtgat aatcatataa accctccttt gttttctgtt
      661 acgctaatta tagcgtacgc acttgagtac gtcaatatta ccaacgagtt catcgtattc
      721 ttaataccta tatgctatcg ttcacaaact tttgttcttt tcttccaaca taccgcctat
      781 aatgctcaaa aagaaatcag cagtggaaca cactacgttc cctaccgctt tatcattttc
      841 aggcttaaat atttttaaaa ttttgtgagg aatccgctta aaaaaactca tatcttcaaa
      901 aaaaatgaca aaatttaaat ttttgaataa catcattttt tagtaaatgt tataatttct
      961 ttataataca aatatcgaaa gaaggcgact gactttgaaa aaattatgtt cattaattgt
     1021 agtagcatta gtttgtatca ttgcattatc agcttgtggt aaagaacaaa ccaaaacata
     1081 tgaaggtgat gttagtggta aacatgtact aacttctatc acatataaag atgataaagt
     1141 tttaaaacag tctacaatca acacaattaa atatgacgat ctaggtatgg ataaagacga
     1201 agccaaaaag ttattcgcca aatctgaaag tattttcaaa gaccttaaag gcgtaaaata
     1261 caaagtagac tataaagata aaaaagcaat tgaacactta gacatagatt acacagaagt
     1321 tgacatgaaa aaattaaata aacgtcttgg tgtttcgact aaagaaaata aagatattag
     1381 ttttgaaaaa cttgaaaagc aattaaagca cagaggttta aaagaaaaag ataaaatgga
     1441 cgacaaatag tttataactt aaaaatgccc tcagataaga ctaaggttac aaaccttaat
     1501 tcatattctg agggctttaa tatttgaagt tcttgtgtga ccagcatcca ctactaatat
     1561 aaaattattt gcagtaacgc taaaatccgc tgctttcaat ttcccgaaat aattaagtta
     1621 actaatgagt tttaatttat aatcatgtat cgtttgtaac tcaccatcga cttttcgata
     1681 tacaatatga tcagcagtaa tttctgtagg actggatacg ccaacagctg ctgcaatatt
     1741 gaataagcct tcatgcaaac ttgttacata gtttgtgaca cgatattgct tttctccaac
     1801 aatcaatgct ttttctttct tcgcatctgt cgttgcaaca cctacaggac acgtattcat
     1861 gtgacattgt tgactcatta tacaaccgac actaatcatc atcccacgtg cgatatttac
     1921 aaaatctgca cctaaaccta gtgcaatcgc aattttatct ggtgtcacta acttaccaga
     1981 tgccgccaat ttcactttat ctcgaatacc atatttttct aacatgccag acacaatagg
     2041 tagagctgta aatagcggta agccaacacc atcttgtaat tcttggaatg ttgcaccagt
     2101 accaccttca ccaccatcaa tcgtaataaa gcttggatac ttatctagtt ccaccatcgt
     2161 acgtacaagt gtttcaattt ctgaaacttt gcttactaca attttgaatc ctactggttt
     2221 ttgacctaat tgctgcaact gatcgacgaa acgaatcaaa tcttcagcat tatgaataaa
     2281 ttcgtaacgg ttaggtgaat tgattgtttt ataaggttca acatttcgga ttttagcaat
     2341 ttcttcgttt accttttcag cttccatatg accaccacga gtcttagcac cttgtgccaa
     2401 cttcagctca aatgcgcgta cgttagataa ctgtgcaacc tctttaaata aaccttcact
     2461 aaaattacct tctttatcac gaacaccaaa taaaccggga ccaatttgga aaatgatatc
     2521 cccattacct tttaaatgat attctgataa gccaccttca cctgtattca tccaagtgcc
     2581 cgctttagct agacctttag ataaagctgt aatggcattt tttcctaaag cgccataact
     2641 cataccagat tgtcctacga tacgttttaa aataaatgga tgttttaaat gttcacctaa
     2701 ttttattgca tggtcatcac ttaagtaata cggatcaatc tttgtcggca cacgatattc
     2761 ttcacgacta aataaacgct cattcgcgat tttataaatg aatgttgata acaatgttgt
     2821 attatctact gaaatctcat tacgttgcat cggaaacatt gtgttctgta tgtaaaagcc
     2881 gtcttgataa tctttagtag taccgaagct ggtcatacga gagttatatt ttccagccaa
     2941 aacgatattt ttataatcat tacgtgaaaa aggtttccct tcattatccc cagaaaataa
     3001 atactgacgt aattccggtc ccattttttc tgaaatatat ctaatacgtg ctagtaaagg
     3061 ataattcctt aatacactat gttgtgattg tcttttatct ttaattaacc aaataagccc
     3121 gataacaata accgtaagca tgaatcctac aacgataatg ttaactataa attgcatgac
     3181 tgtaagaaac gtcattacaa tacctccccc caaaatttca attcaatatt tatgatacac
     3241 cttacaaaac aaaacacaat ggaagcgctt cattttataa aacaatttta tgatatgttt
     3301 ttcattttaa attttaatgt ataaaacata caatacaaag taatatgtgc taaagtatct
     3361 atataataca actatttaag aggtatacta tgtcaaatac aaataaacat tacatagaag
     3421 aagaatacgc taccgaacaa tcgcgttttt tcaaacgtga tattggattt attttccttt
     3481 acatattttt ggttaacatc ttgccgatca ttatcgtctt tttaactttt gggttagtag
     3541 ctgtattcac tcaacaagct ccgtggggtt tctcttttcc tatttcaatg aacattagct
     3601 atttactcgc agttattatt tcactagcta tttttcgtct gatgcatgaa gatgcgttta
     3661 ttccaatgat acgttcgcaa atcgttgcta gtgctaaata taaatggcat attcttatat
     3721 catttacact aagtgtcgta ctctttttac ttttaaatca tttcaatatt ttcgcactaa
     3781 atgtagattc ctttactgtc tttgacattt acccgctcat taaagggtat ggttccatca
     3841 gtttaataac tatattttcg ttaaccatta tattaaatac tttgatttgt caacttattt
     3901 tccgtcatat tttaattcaa gaattaagca gattaatacc tttaaagatt acaatagtat
     3961 tgtccattat ccttgaaact ctgttctact atccatacat taatacatgg tgggaattca
     4021 ttccagcatt gatactagca ggtagtgcga cttacttata tttaaaatca taccgtaatt
     4081 ttatggtgag ctatttctat caattagcag ttatactcgt tttgcatatt ttcatgtaaa
     4141 atttttcaga gtattatcca ttatccatac acatcaaaaa gcacacttaa aagtcagtat
     4201 caaaactcac ttttaagcgt gctttattta gatatactgt cgttctaaaa acgagccata
     4261 tagaatgttt catgtcctag catgaagcac atgaatcgcc atgctcaata taccttttaa
     4321 aatttaaata ttcattaact taatatgctt taattcactt cattaaagac tactcgctgg
     4381 acgtggtgtt ggctttttca agaaactcat aatcattatt acaatgacta acataccaca
     4441 aattagatac atccatttaa atcctactaa atcggaaata ggtcccatca atgcgccacc
     4501 taacgatata cctaagtctg cacaggcaat aaataaacct aacaacatat tacgacctac
     4561 ttttggtaag acgaagctta agtatgatgt taatgttggg tacaccattg cttgcgtcat
     4621 tcctattaat atcgcactac catagaaaat aattgcacct acttgcggac caaatgccac
     4681 tacaaatgac gcgattacta ataatgatag tacagatacc atatatttag gatgccacat
     4741 accatctgac ggaatgtatt tccttaagta aaatcttgcc gcaacaactg cgatggcctg
     4801 tattgtcaaa aagattcccg cattcgcgaa tcctaaactc actgtgtata acggtacaaa
     4861 tgtactaact gcaccaaata caatcgatgc aacaatcatg ataataccac tgttcaacaa
     4921 ctctttattt ttgaaaaatt gcgcaaaaac agttacagcg ttaaacggca ttttttcaat
     4981 cttatctgac gtatcgggtt cctgttcagc aaaggtcacg cgatagccaa agaatgttgt
     5041 tgttaatgcg ataaagataa tgacaattgc aaatagtgaa atattatttg cattccaaat
     5101 acctacggca actaatggtc cgattaagtt tggaatcgtt gaaaatagcg agtacaatga
     5161 tacaccttca gaacgatgtt cctctggtaa tgcatcaata atacctagct gtaaagacat
     5221 agaaaagaat gccgtacaca caccttgcat aacacgtgct acgaaataac cttctaagcc
     5281 agtaaaacca taaataatta aagctatggc attgataatc aaaataattc ttaatacttt
     5341 aatgggaccg acacgagcaa taatttgtcc tgcccatggt cgaaatacca ttgctgttaa
     5401 catgtatgcc cccataacga taccgataac cgtattcgtt gcgcctaaat catgccctcg
     5461 taaaggtata aacacgttta agattgcatt ggcactaaag aacatcaatg ttaatatata
     5521 taatctcaaa aagggccaag ccattgcacc tttcattatc tcactcctta gaatgatagt
     5581 tgtttttcta ttaattctct tgtataagca ttgtcacttt tatgcaatgc gcttgtcgga
     5641 atttgttctt ctatttttcc gtttttaaaa ataattaatt gatcacataa atacgtggca
     5701 gcttgaatat catgtgtgat aaaaatataa ctcaactgac gcgtttcacg taaatgaatc
     5761 aataaatcta atatttgtgt ttgaattgac atgtcgagtg aactaatggc ttcatcaaac
     5821 aaaatatatt tagggttaat acatattgca cgcgcaatcg caacacgttg cgcctctcca
     5881 cctgataaca tattaggata tttatccatg tatgccttag atagaccgac ttcttccaac
     5941 aatgtaattg cttggacttc cataacttct ttaggttgtc catcacattg acacatcact
     6001 tcaaataaga tttctctaac agtctgaaat ggatgtaatg atgacgtata atcttgaaat
     6061 acagcaccaa tttgatgacg tctcactttc ttcttatgca tcggttgatc atttaaggtt
     6121 acacaacctt tatccggttt ctcaatacct aatatcatac gactcaacgt cgatttaccg
     6181 ctaccacttt ctccgataat cgcaatcgtc gcaccgattg gacactcaaa tgacacacct
     6241 ttcacgatag gtgttcgacg acgcttaaaa acatgtgcgc tttgatatga cttttcaaca
     6301 tctttaattt taatcatgta catcacccct catcacatgt ttaaaatgat cattaatctt
     6361 cttcttcgtt gataataaat acttcgtata aacatgttct ggatgatgca agactgattc
     6421 acgtgtccct tgttcaataa gctgaccatt tttcatcaca acaacacggt ctgcaatctt
     6481 gttaataacc gttaaatcat gtgaaatgaa aatcatcgca cagtcaaagt gttttttaat
     6541 atctataaat gcttccagta catcatattg tgtaattgta tctaaagccg ttgtcggctc
     6601 atcagcaatg attaactttg gtttcaaagc taacgctaaa gcaatcatca atcgctgtaa
     6661 cattcctcct gataacatgt aagggtatga ttttaatata cgtttaggat ctttcaaact
     6721 taaataatcc atatattcaa tcaatgtctt ttcaatttct tgtgtagaca ttgacgtatg
     6781 tactttcata gtctcaaaca tttgtttacc gacagtagtt gatgggtcaa aggcacgact
     6841 accttgttgc atgaccatcg caatgtcttt accacggtac tttttcaatt gcgattcaga
     6901 taatgacaac attgatgtac catcaaagat aatttcacct gtcaccccga gtcgttcggg
     6961 attcaaacca ataatcgatt tacaagtgat tgatttacca ctaccacttt ctccaataac
     7021 gcctaaagtt tcacccttag ttaatgtaaa attcacatca ctcacgagtg gttgatctgt
     7081 ccaggtatct gtaatcgtca aatgtttaac tgttaacaat gtcatgattg caccactcct
     7141 tttttcacag aacgaagttt atctttagaa gagatgcggg gatcaatagc aatttgtaaa
     7201 gcatcggata agaagttaaa tgccatcact ataatcacta tggcaatacc tggcgcaaac
     7261 atcatttcag gatgtgtaaa catcactttt ctagcttcgt taagcatcat gccccactct
     7321 gcagtaggcg ctttgacacc taatcctaaa aatgaaaagc cagatatttg caagatcatt
     7381 gaacacatcg agctactaga gatgatagca atatctgcta atgttaatgg cataatatgt
     7441 ttgtgaataa ttttcatatc attcataccg attgttttag caaatcttac atggtcagaa
     7501 gcagtgtact gcataacact tgtacgtata acacgacaga accatgccca acgcgtcaaa
     7561 ataaatgcca tgataatatt ttcggcaccc attccaaaca atgcaattaa tgctaacgtt
     7621 acaacataac ttgggaatgc caacataaca tcacacgcac gcatgattaa ggcgtcaaca
     7681 aacccttgga aatatcctga taagaatcct aaaatagatc caataagtac agaaacaaat
     7741 agcgcaacaa agacatataa caaacttggt ctaatcgcat aaattaacct agttaaaata
     7801 tctctaccta aatggtcagt acctagtaga tgttgaaaac tcatgccagc aaatttgttt
     7861 gctgtatcga tatggttagg atcataaaat gtcacaagtg gtgctgctaa tcctaaaaag
     7921 acatataata caataatgcc taaagcaatt actgcacctt tatcttgtaa taatcgtttt
     7981 aaaattatca tcgtgcgccc tcccttaatc ttggatttaa tagcgcatta atgatatctg
     8041 ctaatgtatt aaatacaata aataataccg ctacaattaa tacatatgct tgaatgactg
     8101 gaaaatcgtg ttcaagtatt gcttttaaac ttaattgacc tagtccaggc catgcaaaga
     8161 tatactcgat aacaactagt ccacccatta tcattggtat agacatacaa aagattgata
     8221 ccgcaacttg taaagcatta cgcaacacat gcaacattaa tgtgatagat ttcacaccgc
     8281 ttgctcttaa ataaagtaca taatcttcat ttaattgttc caccatcgag cgtctaacat
     8341 ttctaaagta aataccagca taggcaatcg taataacgat cactggcaat atgtaacttt
     8401 ctggacctgt taatccagaa gtcggcaata tgtttaactt cactgaaacg taaataataa
     8461 gtattgaagc tatccaatat gatggtaatg cagttagaaa gaaagccact gaacgtatcg
     8521 cacgatcagt gaactttcct ctttttaatg cactaactac acctaaaata attgatgtaa
     8581 tcatcaccat aacacttgaa attattgtta atttcaatgt attcataaat gctggaccaa
     8641 tacgttcagc aactgggtca cctgtaatgt agcttgtacc aaaattaaat tgcatcgctt
     8701 caagtaacca atttttatat tgaattaata atggatcatt gaaaccgtac ttctcattcg
     8761 tttctgcaat caactctggt gttacatttg gcgtcccttg tgcatgtaaa attgtcacag
     8821 ctggattttc atttgtaata tacgtcaata gaaatgtcat aaaacttact acaatcatca
     8881 atggaaacat gagcgcaata cgttttaaga taaatttgaa catctaattg ctcctttatt
     8941 tatactgcat ttcattgaat ggtaattcat actgtgattg tgtgaatgat actttttcta
     9001 aatcttttgg tgcaacaact gtcatactac cgtgtgaaat agggataaag ataccttcat
     9061 catcaatttg tttcaaaatg tttttataag cgtctgaacg ctctttaccg ttttggattt
     9121 taaatgcgtc atcaatgctg ttgtatattt tatctttgtt ctcaatgcct gatgttgcac
     9181 tttcataacc atttttcgct ttaaatgctg caatagtact ttgtggatcg tacaataatc
     9241 cccaagtttg gttgaacatt aagtcataat caccagaagt acgacgttca gcaattttat
     9301 ctgatgtttc gccattgatg tttaacttaa tacccatttt cttaaattct gcttgtaagt
     9361 attctgcttg ttctttttga cttgaagaac ctttgtcata gtacattgcc atttcaaggt
     9421 ttttaccatc tttttgacga acatcgctgt ctttaccttt cttccaacca gcttcatcta
     9481 ataatgattc tgctttttta aggtcatact tacgtgttgg catatcgaaa ttaatgtctg
     9541 ttacattttt cgcaaataat tgagttgctg gtttttcttg accatctaaa atttctttgg
     9601 caattttatc tctgtttacc atatgaccaa tcgcttgtct gactgttttg tcactcacag
     9661 cgttatcttt tttaccagaa ttgacaacta acattttcgt attcataggt tgactacgct
     9721 taacttgata gtcacctgta tctttcaatt gttttaaaga gtctttgtct aagctatctg
     9781 tacctctatc atctgtgaag gcaaagttcg tttcaccttt tttcattgat aggaatgctg
     9841 tttcaccagc aggcattact tttgcttgta ctttgttaag tttagacttt tcgccccagt
     9901 attgatcatt tttgttaaag tctgcagact catctttttt gtgttcacct aatttaaatg
     9961 gaccagtacc atcgaacttt ttaacgccat cttttgttgt accgttttta aagtcttttg
    10021 gagacacaaa tacatatgga cgaggcatcg ctaattcagc caatgcaggt tgatatgctt
    10081 ctttcaaatt caattcaacc gtgtacttat ctttaacttt aacattgtca attaatgtcg
    10141 aaatctttaa ccaagaatgc aattttttgt tttcttgaac tgcgtcaata tttttcttaa
    10201 ctgcgtcagc atcaaatggc gtaccatcat ggaatttaac gtcatctctc aaatggaacg
    10261 tgtatgtctt cccatcttca gacacatccc actttttagc tagtaaaggc ttaataccat
    10321 ctttcgtgtt acgtacaagc ggctcgtata tcatactttc agcagacatt gatccaccgt
    10381 aaacatgcgg attcatatca ccgatatctt taaccgtcgt atacgttaat tgcttgtttt
    10441 cttttttctc ctctaaacct ttattaccgc cacaaccagt taaaattagc cctgatgcaa
    10501 gtaacattgc actcatttta gttagttttc tcatttgctt ttcctctttc taaattgata
    10561 agttgcatta cttaatcaaa tcgtaatgat tatttttctc aattatttgc ttatttttat
    10621 gtaaatgtca aacataattc aataattttt ttctattata aaacttcaaa cccttattat
    10681 gaaagcgttc tattgatttc caaaaatttt gtgacgagtg ctttatctcc ttcaaataga
    10741 tttgtattaa attgagaaga taagtgttga tcttgatgca tatccttgta cgcctggcaa
    10801 cttgcttcat agcgagttag gaactgatca atcattggcg ttttgatacc tagcattttc
    10861 ccaatatgct gaatcatcgc cgttctgtaa taatcttcac ttggcattct tggaatttga
    10921 acaacatcct gttcattttt atagacttgc ttaaatggta cagctgaaaa atcaaagtaa
    10981 tgtccgtttt cgtctggctg tgaaaatgga tcaatgagga ttgcggtata tcttacataa
    11041 agcagatatt cttgtaagat atctggcaag atttcgaaat gctcaatatc accttcatcc
    11101 aaagtttcag gacgtactgg ataattttcc ttcaccataa attgaagcag gttgactgac
    11161 ggcactctaa atgcttgtaa aataaccatc atttccttcc acattaaacg catttcacgg
    11221 attagtgtca tcgttatcgg tccttcagga aataacttat acacataaac cggtacatct
    11281 gttccttcga aaatggcttt caatgaaaag tcattcataa atagtggtgg gtgcacataa
    11341 agcgaactgt ttcgcgtttc agcatgcagt ggcgattcaa cgacttctaa ttgaatcttc
    11401 aattgctcag ctaaagcaga gattcgttga cacattgttg agtttgaatg tgtcgatccc
    11461 atgtacaatt tcttttttac acctgttgtc aacacatgat taggcgcttc tttatcaaca
    11521 atacgtgtat cgccaagata agttgagaat gaaatcactt cgatatcttt attaaattta
    11581 gacataaatt gttcgacaat catttgcgaa ccaaatgtcg gtgatattaa aatgacatgt
    11641 ttgacacttt gcaaagtttc taacgacaat tgctgtagtg tgtcataata agcatctgct
    11701 gtgcatgcca tgacaaccgt ttcgtattca cccttaacgt ttttaacatc tttatacaaa
    11761 cgattaattt caaacttacc ttccagatgt tgatgcgcct cgttttgtat tttgacttca
    11821 aattgtttcg cttttttata cgcttgatat aagcgttttg atttttctga tgttgaggca
    11881 cgtccaacca tatcaatctc ataatctgat tttaaatagc aaatattcgc taattggatt
    11941 gcgaccggac cagtgcctat cattaataat ttagacatcc gtaataccta cctttatagc
    12001 tgctttttta taaagtgcta tatcaaaaat ttgctgtggt ctcatatgtt tattcacaca
    12061 ttgccactta tcttccgctg tttcttgtga cggataatta aatattgctt tgatgccatc
    12121 accaaagcgc attgcaacta cgacattttc atttgttaaa tcatataatt cttctaaaat
    12181 gctgtacttt aaaggaattg tcgagctgaa tatgatatgc gtcacatctt tgatatcttt
    12241 aagttcagat accttttgat ccgtaattgt tatatcttca tttggtgcta agacgttaac
    12301 gattctgcgc cctaggtcaa cggcttgtgg atcaatatca ataccgataa ctgaagcacc
    12361 tgtttctttt gctacttgaa ttaacgtcat tggatatgca cctgaaccaa ctaacaataa
    12421 tttgtcattg ctagctatac tacattgtcc aaactcttca tcaatacaat gttctatatt
    12481 gtcaaagtaa cctgtggtag acgcctgtcc gtctaataga cgtctcgctc taatgacttc
    12541 cacttgttta acacactgcg ctgtaatata ttgcagtgat tttattaatt gtgctttttc
    12601 ttcaacgtca cgccaagcat taaattgttg ttcataaata ggatttaaaa taaattcact
    12661 atagtcatcc atcaatgttt ctaatgcttc gatatattga tcgtcttgta atacacgctc
    12721 gtaatgcgct tcaaactttt ctaaatattg ttgtaatatc aatttgattt cattattaaa
    12781 gttattcatt gtaggctcct tattctatat atgcttttcc tgtagctaca gttgtaacct
    12841 gtcctttaat tgaagtttga tatcccaatt gatgacatcg ctttgatgtc actaaaatac
    12901 tgccccctgg ctgatgtact gtaaaatctt tgcatgcgtc attacgttga taattattaa
    12961 aaaccccaat tgatgctgta ccagaaccac agctattttc ccaaattaaa ctttgaattt
    13021 ctggtatata gattaatggc tgtaaaaatt gacgttgttc atcaaaaagc atcataccta
    13081 ctgttttata tttgtgactc cattgttgct cacgcacaaa cgcttcaacc aaatgttgaa
    13141 tttctgttgt tacttgttta actggaatca cataatgtac atatgtttca taaataattt
    13201 ctattgcttt ccatgaatga ttacccatat taattgttgt tggcacaaca cgatgggctt
    13261 gtggcatttg aacttcatag tattggcaat catgaattgc gcattgcact aaatccgaac
    13321 agccagacac cttcacctta aactgttggt ctttaagcaa atgactttcc tgcaaatgat
    13381 gtatatatga catcgtcgca ttaccgcaaa attcattacc gctcataact aagtgaaaat
    13441 cattaccatc atcattttgt gttgattcta taaagcctac ctgttcacag catacatgtg
    13501 ttgcggccat caactgattg gcgatagatg catattcact agcatcatgt tttgaatgaa
    13561 caagtatcgt catattcccc gaaggattat acttagaaaa ttctataacc tgccgattca
    13621 tacgacactc ctttagatgt atttttagag cctctgtaat tttaattact agaggctctt
    13681 atgcagttgt tgcgaagcaa acaactgtat tcctctaaac ctgtaatttt ttagaagatg
    13741 aacctaatag ctctatctag taggtaagtc atgctaattc gcaaaagcga aaaccatggc
    13801 tcttatgcag ttgttgcgaa gcaagcaact gtattcctct aaaactgtaa ttttttagaa
    13861 gctgaaccta atagctctat ctattaggta agtcatgcta acccgcaaag tggaaagcat
    13921 gacttagtaa tcggttgttg tatcaaaatg caaccgtaaa ccttatttaa agttgcttct
    13981 tgtggctcta caagcgaatg cagttgtccg ttaactacat tcacttagat gcttttactg
    14041 ttttcacctc aactaaaact tcaaaaaagt tgcagaaaac aaatcgtaac tattacgatt
    14101 tgattatata ctatattagt tgagaatcat tgtcaataaa ggttgtaaat gaatgcacat
    14161 ttatattttg ataatccttc cataaacttc gtattctcag cacattcaag tctttttgaa
    14221 aatttttatc aataattgag tagaagttcc gttctagtca gttcaaaatt ttacgtaagt
    14281 tgtctctgcg tattgcttta gtgagaataa cccacacttg tggaaacgtc cttttgttaa
    14341 tctacaatgg atacccttgt ctttcgctaa catttcctat taccaatcct gtaacggatt
    14401 ttcatcgtca agttgttgcc catacaatac gcaccaaaaa agctttgtac aatttcgtac
    14461 aaagctttaa aaatttaaat aaaaatatca ttgttttaat gaactagcca tatcagattt
    14521 tatttcgttt gctaatactt gtaattcagg gaagtaatta ttagcaaagc caattattat
    14581 tttatttttt acaaacgtgt taatctctcg atcttctgga atcgtatttt ctattaaatt
    14641 caaaagctca ctatgactta tgttctctat atttactgta tttaaatatt ttaaaatcac
    14701 aagatacaat tcatcaaata cttcatgttc aaaatcaaag tccaataata ataaactaac
    14761 ggaattagtt tctaactgat tatctccaat tttatctgca atctctctta tagcttgata
    14821 tagcaatcta aaactttctt tttcttttaa cataaaattc tccttaattt attagaatag
    14881 ataaaataca gcttatttta aagatactcc aaaagtttga actcgagatt ctgataaatt
    14941 agatgcacga cctacaatac gttaaaacaa gctttaagca atacacttca aatgaacaat
    15001 atgtgatttt caaagattaa ttaatctccg ctttaaatgt ctttcattgc gacaatatgc
    15061 atacattgcc tacttcatgc caaattcttt tccactggat atcgatttta aataattatt
    15121 ccaatgtccg cttccacgaa tcatcactta acatctcgcc aggccatgtg taagccatca
    15181 caccaccatc aattcgaatc gtctctccag ttatgaatga actgtcgtca gatgctaaga
    15241 agactactaa tttagcaact tcttctggtt tacctaaacg tcccagcgga gtcatccatt
    15301 tttgattttc tctaaatgtt ttacctgcat catcctcact cgtacctgtc agtttatcta
    15361 ctaacggggt ttcaattgta cctggtgcaa tcgcattggc tcgaatgcta tcacggccat
    15421 actcaattgc gattgatttt gtaaaattaa tcactgcacc tttcgcagca ttatatccag
    15481 agcgatacaa gtctgctgct tgtccggaaa atgaagacgt attaacaata gagccacctt
    15541 gattcatcat taaaggtaac atcattttcg tcattaaaaa tgtcccacgc atatctacat
    15601 tcataatctt gtcatacaca tctattggat actcatgaat tctatcagcc gcattatcca
    15661 caccggcatt attaaacaac acatcgattc ttccaaactg ttcctttatg tcagacacaa
    15721 agtctaccac ttgttgttcg cttgcattat ccacattata cgccttcgca ttgtcaccat
    15781 tacttttaat tttatcgaca gtctccgata ccgcttcagc tatgtctacc gccaatacat
    15841 acgcacctta ttgagctaaa gcgattgcag aagcttgacc gatacctgta cttgctcctg
    15901 ttacgactgc tactttattt tccaaacgtt tcattcagtc actcctatac aacatatctt
    15961 aaacttacag tccttatttt ccactgttcg atgcttcata aacctaatag ataagtcttc
    16021 aggcagtctt aactactctg ctatacactt tctattgaat aactcaaaat atttaacaat
    16081 attagttttt ttattttctg aaaattgtta cttttgagag tattttttgt tacactttaa
    16141 atatttaaca aaggggggaa taattgatga catcaaaaca tcaacaaaag gggtctatcg
    16201 tcaatcgttt cttgaatagt gttgaaaaaa tcggaaataa attgccagat cctagcgtct
    16261 tattcttttt aatgtgtgtg ggcttagcca ttatgacatg ggttatctca ttatttaatg
    16321 tatctgttaa gcatccaggt acgcatcaaa ccatttatat taaaaatata attagccatg
    16381 atggatttac gatgataatg aatgatacga ttaaaaattt ctcagagttc ccagcattag
    16441 gcttagtact agcagtgatg attggtattg gtgttgcaga gaaaacagga tacttcgata
    16501 agttaatgat ttctgttgtg aatcgcgcac cacgcttctt aattttaccg actattatat
    16561 taattggtat tttaggtagt acagccggcg atgctgcgac aattatcttg ccgccgcttg
    16621 cagcaatgct ttttattaaa attggctatc accctatcgc tggactaacg atggcatatg
    16681 cttccgctgt tggaggattt gcagcaaata tagttgttgg tatgcaagat gctttggtct
    16741 attcatttac agaaccggca acacgtatcg tttcagattc tattaaaaca aacgttgcta
    16801 tgaactggta ctttatcgca gcgagtgtcg ttgtattact tcctacgata ttgctagtta
    16861 caactaagct cattattcct agattaggaa aatatgatga cagtttaatg catgatgacc
    16921 atgaagaaac atcttcacat attactgaca aagaagcaca tgctttaaaa tgggcaaata
    16981 tcagtttcat agtcacaatt attttattaa ttattacagc cattcctgaa catagctttt
    17041 taagaaatgc taaaacaggc agcttactag acgatgcgcc attaattaac ggtgtcggat
    17101 taattattct tgtcgtattt ttagtacctg gattagttta tgggatttta agcaaagaaa
    17161 ttaaaaatac aaaagactta ggtaaaatgt tcggcgatgc tgtaggatca atgggaacat
    17221 ttatcgttat tgtgtttttt gcagcacagc tacttgcgta tttaaagtgg agtaatttag
    17281 gaattatcgc tgctgttaaa ggtgccaaat tattagaaca tcaaaacggt attgtattga
    17341 tactaggtat tattgtactg agtgcaatgg tcaacatgtt aatcggaagc gcatcggcta
    17401 aatggggtat tttaggaccg atattcgtac caatgttaat ccttatcggc tttcatcccg
    17461 cattcacaca agtcatttac cgtgtaggcg attcaattac caatccaatt acaccgatga
    17521 tgccgtactt acctttatta ttaacttatg cacaaaaata cgataaacgc atgaaactcg
    17581 gagccttact ttctagttta atgccgtatt caatcgcgct aagtattgta tggacagtat
    17641 ttgtcatcat ttggttccta ttaggtatcc ctgttggacc aggtggaccg atattcgtta
    17701 aataacagta ttcaaaggga taaattaaaa tgccactata caagatttga cctgtatagt
    17761 ggcatgattt gttatgatat agcgttcgag atgaaactct gcatttatat gaacaattat
    17821 agtctatcac taataatatt taatctgttc caaccgttaa tttggtttac aacaaagaca
    17881 agatcaataa tttgttcttc atcataaaat gattttagcc ggtcaataat ttcaaacttc
    17941 ttaaagtctt tgattgaatt taacatttct gcaaattcaa gcgtcacttt ttcttgatca
    18001 gtaaataaat ctaaatgtct aaaactcaag acttcatcaa ttttcatttg tgttacaccc
    18061 aattccttta attctttttt atggatatca acacaataat gacaaccgtt aattttagaa
    18121 attaataact taatcaatgc actaagttca tcattaacgg gtgattgatg aataaacttt
    18181 tcaccttggc tcattacaga cattaactct ccattttgtt tcttgtaatt atacggcatt
    18241 tgcaatctct cctttacatt gtttaatacc aatccagtga tactacactt cgaacaatat
    18301 tggatagaat ttgaaatt
//
LOCUS       DAKTLH010000043        17862 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.043, whole genome shotgun sequence.
ACCESSION   DAKTLH010000043 DAKTLH010000000
VERSION     DAKTLH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17862
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.043"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            254..958
                     /locus_tag="O0394_001227"
     CDS             254..958
                     /locus_tag="O0394_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCY0499313.1"
                     /translation="MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYS
                     IAIHSDVTSNKKRYYKSYSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTI
                     VPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALAQKYREIDIKLSTEDKKL
                     LSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISED
                     DMNEIEKQLKIKFFSN"
     gene            1351..1887
                     /locus_tag="O0394_001228"
     CDS             1351..1887
                     /locus_tag="O0394_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499314.1"
                     /translation="MHNNNEFNKKLKDFIGSDKRMALVKGYVNEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNTIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNDWSFNTLELEKIVDETIIYDLKQE
                     DPKQYEILYKNKHGKLPY"
     gene            complement(2000..3541)
                     /gene="guaA"
                     /locus_tag="O0394_001229"
     CDS             complement(2000..3541)
                     /gene="guaA"
                     /locus_tag="O0394_001229"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCY0499315.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCRGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(3566..5032)
                     /gene="guaB"
                     /locus_tag="O0394_001230"
     CDS             complement(3566..5032)
                     /gene="guaB"
                     /locus_tag="O0394_001230"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCY0499316.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(5070..6338)
                     /locus_tag="O0394_001231"
     CDS             complement(5070..6338)
                     /locus_tag="O0394_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCY0499317.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILLLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(6338..6916)
                     /gene="xpt"
                     /locus_tag="O0394_001232"
     CDS             complement(6338..6916)
                     /gene="xpt"
                     /locus_tag="O0394_001232"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCY0499318.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(7147..7249)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7429..7836
                     /locus_tag="O0394_001233"
     CDS             7429..7836
                     /locus_tag="O0394_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCY0499319.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDKSSHQEVHENNSAYEEIDITHFAEASKGPKA"
     gene            7979..8641
                     /locus_tag="O0394_001234"
     CDS             7979..8641
                     /locus_tag="O0394_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499320.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            8759..9715
                     /locus_tag="O0394_001235"
     CDS             8759..9715
                     /locus_tag="O0394_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499321.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKALAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIEKASVEQSKNDNAAETSNKEATVDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
     gene            complement(10506..10607)
                     /locus_tag="O0394_001236"
     CDS             complement(10506..10607)
                     /locus_tag="O0394_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499322.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNSVVIGGKGK"
     gene            10692..12080
                     /locus_tag="O0394_001237"
     CDS             10692..12080
                     /locus_tag="O0394_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCY0499323.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLIYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FDSNDNAALTSH"
     gene            complement(12161..12916)
                     /locus_tag="O0394_001238"
     CDS             complement(12161..12916)
                     /locus_tag="O0394_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HCY0499324.1"
                     /translation="MSEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            13408..13977
                     /gene="ahpC"
                     /locus_tag="O0394_001239"
     CDS             13408..13977
                     /gene="ahpC"
                     /locus_tag="O0394_001239"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCY0499325.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            13993..15516
                     /gene="ahpF"
                     /locus_tag="O0394_001240"
     CDS             13993..15516
                     /gene="ahpF"
                     /locus_tag="O0394_001240"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCY0499326.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKDLLTEIT
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKKLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMNTGEEHLLNLDGIFVQIGLLPNTSWLNDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            15646..15888
                     /locus_tag="O0394_001241"
                     /pseudo
     CDS             15646..15888
                     /locus_tag="O0394_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15961..16587
                     /locus_tag="O0394_001242"
     CDS             15961..16587
                     /locus_tag="O0394_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HCY0499327.1"
                     /translation="MKKRLLLSTFLASTLILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            16842..17225
                     /locus_tag="O0394_001243"
     CDS             16842..17225
                     /locus_tag="O0394_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499328.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFTSTIITLAIGGKSVEKNDSP"
     gene            complement(17291..>17862)
                     /locus_tag="O0394_001244"
     CDS             complement(17291..>17862)
                     /locus_tag="O0394_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCY0499329.1"
                     /translation="LVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLCDYFK
                     EKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISANPKYD
                     KYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAHQVVI
                     RCLMVYFNKVSRAEAVDLKVENCKPYIIE"
BASE COUNT         6048 a         3293 c         2815 g         5706 t
ORIGIN      
        1 ttagattgct tttagcatta ggaattcgct tataattaga tgttcaatat agactttttt
       61 acacatacat gaactataaa aagcgaataa atagcaggtc attgcgaaat ttgctaaata
      121 caaaattgat gagttccacc taataaaaat tgttggtttt atagtaacat ctattttcgt
      181 accattcatg ttaatttcag tgctaataat gctgatataa agaagaataa attgattagg
      241 agaacactat ttaatgtcta aaaatatcaa tcaagcaaat agtaaattaa atacgtcaaa
      301 caaaaaacta aaacaagctt attcaaaatc cgacagtaaa aacatgaaag ttatatacat
      361 gcctcattgg ctagagtttt attcaattgc tatacatagc gatgttacaa gcaataaaaa
      421 aagatattat aaatcatata gtagaggaac agtggtatat gtaaaactag ggagtaatat
      481 tggaagtgag ttttctggta atcatttttg tgtaatatta gataacaaag acaataaagg
      541 aaaagaaaca gttacaatag ttccactttc ttccaaaggt aataaaaatt atttgaagtt
      601 aaacgaatcg gtgctaaatc taacagcaac agatttaaaa aaacagatta tcgatatcag
      661 tagtaaagta caagcattag cccaaaaata tagagaaatt gacattaaac ttagcacaga
      721 agataaaaag cttttgtcta acttaaatca aaaagctaat gaactgtata gagtgatagg
      781 tgtttattcc aagcacaaag ggaaagatac atacgttaat atatcagcaa ttacaacaat
      841 aagcaaaaga cgtataagca aaataaatga ttctgatcct actggaacga taataatttc
      901 agaagatgac atgaatgaaa tcgaaaagca attaaagata aagttctttt ctaattgaat
      961 tgtaatatta catctgatat aattgattta caaattcccg gtaaccattc cggcttcatt
     1021 ctcttgaaga atgacatatt ctcagcgttt tagctgaagg tcagatgata cgtcatctgg
     1081 cctctttttt tatataaatg ccctttaagt gaaatataca acaatctgca atcaatatta
     1141 gacatcacaa aacacacaat aaattacaca atgtgttata agccacatgt cactattatc
     1201 gcagctagct gcgtcactca aaacgaatac ggaacgcgtt ggttatttca atcataatat
     1261 tactctgcaa atacacagcc ctgtaaccga gtaaatggat agagatttga acaaatgaaa
     1321 acaatcaact aatggaaagg ataaaatatt atgcacaaca ataatgaatt caacaaaaaa
     1381 ctgaaagatt ttataggtag cgataaacgg atggctttag taaagggtta tgttaacgag
     1441 tataaattaa caactgtttt aagagcttta aataattccg attacacaaa agggacgata
     1501 cacactcgtt caataggcaa cctaaaacaa atagtagata atcgaattat tcctaaaaat
     1561 ataactcaaa acacaatctt caaattgagt aatttaacct tagaggttaa cctctatgaa
     1621 agaaacacaa tttatcacgg agactttgct atttactatc ctgtagaatc tgctctaata
     1681 atagaaacag atactcctag gctaattaat catataaata acaacccagt ccaaaaaata
     1741 tttataataa caacaaatga ctggagcttt aatacacttg aattagaaaa aatagtagac
     1801 gaaactataa tttatgattt aaaacaagaa gatcctaaac aatatgaaat tttatataaa
     1861 aataaacacg gaaaattgcc ttattgatga attaaaacgc aaacttagaa caccttaccc
     1921 cttttccgtc cttattgtaa ttttccaact actcaaatta aaaaccaccc gtgcaaacag
     1981 gtggttttat tatatataat tattcccact caattgtgct tggtggtttt gatgtaatgt
     2041 catagactac gcggttgacg tgatctactt cgtttacgat acgactagaa atcttttgta
     2101 agacttccca atcgatgcgt gcgaagtcac ttgtcatacc gtcgatagat gttactgcac
     2161 gaatacctac tgtgtgatca tacgtacggt agtctcccat aacacctact gattgaatgt
     2221 ttggtaacac tgtgaagtat tgccaaattt ctctttcaag accttcttct ctaatcactt
     2281 ggcgtaaaat cgcgtctgat tctctaacga tttctagttt atcttcagta atttctccaa
     2341 gtacacgaat accaagacca ggtcctggga atggttgtct ccatactaaa tgttctggaa
     2401 tacctaactc aatacctaat ttacgtactt catctttaaa caatgtattg attggttcga
     2461 ttaattcgaa ttccatgtct tctggtaatc caccaacatt gtggtgtgat ttgattgttt
     2521 gtgctgtttt agtacctgat tcgatgacgt ctgtatatag tgttccttgc gcaaggaagt
     2581 ctacaccttt cagttttgat gcttcatcat caaatacgta tacaaattca ttaccaatga
     2641 ttttacgttt ttgttcagga tctgaaacac cttttaattt attcataaag cgatctttcg
     2701 cattaacacg aataatattc atgttgaaac cttcaccgaa ttgctccata accatgtcgc
     2761 cttcaccttt acgaagtaag ccatggtcta caaagataca tgttagttga tcacctattg
     2821 ctttatgcaa tagtacagct acaacagatg aatctacgcc gccactcatc gcacataata
     2881 cacgacggtc tcctacgcgt tgacgaatct tttcaatttc gatttcgata aagttttcca
     2941 ttgtccattg acctctacaa tcacaaacac gacggacaaa attatttaat aaatcattac
     3001 catattctgt atgacgtact tctggatgga attgaacacc ataaatgcga cgtttcttat
     3061 cttcgattgc tgcatagtct gtgcttgggc tatcagcgat aacttcaaag ccttctggaa
     3121 tttcaataac tttatcagaa tgactcatcc aaacagtttg ttctgctggt aagccagcga
     3181 ataactcatc tgacttcgca ttaatgattg ctttaccgta ttcacgttca ttggcacgtt
     3241 caactttacc acctaataat ttagtagtta attgcatgcc gtaacaaata ccaagtactg
     3301 gaattcctaa attatatatt tccggatcaa ttgtaaatga accttcttca taaactgaat
     3361 ttggaccacc tgataagata ataccttttg gattcatttt cttaatttct tcaattgaaa
     3421 tttcatgatc gtgtaattca ctataaacgc ccatttcacg aattcggcgt gtaattaatt
     3481 ggttgtattg gctaccaaag tctaagacaa ggattaactc ttgttctttt gccatttcca
     3541 tatttgtcgt tctcctttat cttaattaga atgagtagtt cggtgattct ttcgtaattt
     3601 gaatattatg tggatggctt tctgctaaac cagcaggacc catacgtgta aattgtgctt
     3661 cttcgcgtaa ttctcttaaa tcgtgtgaac cagtataacc cataccagca cgcacaccgc
     3721 ccattaattg gtaaattgta tcttgtaacg cacctttata agccgtacgt ccttcgatac
     3781 cttcaggaac aaatttctta ggcgctttgt cctcttggaa gtaacggtcg tttgaacctt
     3841 tttccatcgc acctaaagag cccataccac ggtatacttt atattgtcta ccttggaaaa
     3901 tttctgttgc gcctgggctt tcttcagtac ctgctaataa gctacctaac ataaccgcat
     3961 gtccaccagc agctaatgct ttaatgatat ctcctgagaa tttaatacca ccatcagcaa
     4021 tgatagcttt accatgtttg cgtgcttcag ttgcacaatc ataaattgct gtaatttgtg
     4081 gtacaccaac acctgctaca acacgcgtcg tacaaattga acctgggcca ataccaactt
     4141 taacaatatc tgcacccgct tcaaataaat cttttgttgc ttctgcagtt gctacgttac
     4201 ctgctactaa tgtgatttct gggtaagtct tcttaatatg tttcacttga tcgataacac
     4261 ctttagagtg accatgtgct gtatcgataa ctaagacatc cacacctgct tcgactaatt
     4321 tttgagcacg aatatcagta tcttttgaaa taccaattgc tgcggctaca agtagacgac
     4381 catgttcatc ttttgctgca ttagggaatt cgataacttt ttcaatatct ttaatagtaa
     4441 taagaccttc tagacgtccg tctttaacta atggtaactt ttcaatctta tgtttttgga
     4501 gaattttttc tgcttcttca agtgttgtat tcactggagc tgtaattaaa ttttcttgcg
     4561 tcattacatc tacaatttta atcgagaagt cttcaataaa acgtaagtca cggtttgtta
     4621 aaatacctac taagttgcga tcttctttat tatcaacaat tggtacacct gaaatacggt
     4681 atttacccat taatgcttct gcttcataaa cgctttcttc tggcgttaag aaaaatgggt
     4741 ttgaaatgac accattttct gagcgtttta ctttttgaac ttcgtccgct tgttcttcaa
     4801 cgcccatatt tttatgaata acacctaaac caccttgacg agccatagca atcgccattt
     4861 tagattcagt tacagtatcc ataccagcag aaataactgg aatatttaat ttaactttgt
     4921 ctgataattg tacgcttaaa tcaacgtctt tcggtaaaat atcagattgt gctggaatta
     4981 ataacacatc atcaaacgtt aatgattctt ttgcaaattt actttcccac attaaaaaca
     5041 gcctccattt ttcaaattaa ttagttatat tatttcacat tttcttccgt ttgtttatac
     5101 tttattccat taaaaaagaa attaagaata attgcagaga ttgcgcctaa aacaattccg
     5161 ttttgagtca accatgcaaa ttgttcacct aaacctttaa atgcttgtgg tacagcgctt
     5221 ataccagtac ctaatcctac tgatacagcg ataattaata aattgttttg atttttaaaa
     5281 tcgatatgtc ctaatatact aacaccatat gccattacca tgccaaacat agctatcatc
     5341 gcaccgccta acacaggtag cggtatgata tttgctaatg cgccaagctt aggtatacaa
     5401 ccacatataa gtaataacac gaccatgccg tatataacat tgtttttctt agcgccggat
     5461 aaagaaacaa gtcctacatt ttgcgaatag gctgtatacg gaaatgaatt gaatatagaa
     5521 cctaacacta tcgctagacc ttccgcagta taacctttac gaaaatcttt tctttctaac
     5581 ttcttaccgg taatttcact taacgcatga tagacacctg tcgactcaat taaactaacg
     5641 atagctacaa taaagaacac taacgtcgat gtcacatcaa agctaaatcc agagaatcta
     5701 aacggcactg ggatgcctaa ccaaccggca tgattgactt gattaatatc gaccatccca
     5761 agtaagccag cacctatcgt tcctaaaacg agtccaatta atatggcaat actcttaata
     5821 aatccagttg tgaatctttg taaaagaaga ataatgatta atgtcattaa acctaacaaa
     5881 atgttcttaa catctccata gtcctttgca ccttgacctc cagctaagta attcattgct
     5941 actggcatta aattgatacc aatgatagta acaacactac ccgttactac tggtgggaag
     6001 aattttacaa gatgtgaaaa gaaaggcgcg atgataataa ctaatatccc tgataaaaat
     6061 agcgaaccat aaagtacatc tattcctttc gtttgaccaa ttaaaatcat gggcgcaaca
     6121 gccgtgaatg tacatccaag aacgattggt aatcctgttc ctgttacttt attggcttgt
     6181 aaaaatgtgg caaccccaca cataaatata tctactgtaa ctaagtaagc gatttgttca
     6241 ggtgtaaact tcaaacttgt accaacaatg attggaacta agatagcacc tgcgtacata
     6301 gctaaaagat gttgaacact taggattaaa tttttcatta ttcttctccc accaatgtca
     6361 ctttgtttcc ttctagtgaa gcaaccttgc agagagaaga aactgttaaa cctgcttctt
     6421 ctaaacgttg atgcccattt tggaaactct tttcaacaac aataccaata ccagctgtct
     6481 tagcattcgc ttgctgtgcg atatcgtata atcctaatga agcatcacca tttgctaaaa
     6541 agtcatcgat gataagtaca gtatcttctt ctgataaaaa ctcttttgaa acaatgaccg
     6601 tacttgtttt atttttagta aatgaatgaa tagatgtttc ataataacca tccgtcaaag
     6661 tgctaggttt tgcttttttc gcaaataaac atggcacatc aaaatgcagt gcagccatga
     6721 ttgcaggtgc gataccggaa gcttcaatgg ttaagatttt agtaatccct ttatctttaa
     6781 attgctcgta aaaagtgcga ccaacttcat tcattaactt tgcatcaatt tgatgattta
     6841 aaaatccatc gacttttaaa atcttctcat caatgacaac gccgtcttcc tttacttttt
     6901 gtcctagtaa ctccactctt aaaacctcct cagatttgtg tgaaacattt tgctcttaaa
     6961 ttggtgctag atacaaaaaa atccccaaac taaataatag tttcagggtt tatgagtgaa
     7021 cgaacatgca taacgaattt gtcatgcaat caatgtaaaa gaagtttcat caaataaact
     7081 gtgaccatca tataaaatga tataaatcac ccaccatggt tacaatttaa tggctgaagc
     7141 tactcctagt attgtgttgt tactcatagt catgtcgttc aaggcaacat ggtagaaact
     7201 tctaaagcca tattctttag attatatgag tttatgtaaa ttatttaacg ataatagcaa
     7261 attttcggca ttttttcaat aactgcttag gtaatctttt aatagtttta taaatgtaca
     7321 gagacgttta atgcgaatga agacttataa cttaataatg taagggtttt actttataat
     7381 aagaagggta aaagattaaa taaacaaatt tttaaagggg cgacaactat ggcagatatt
     7441 actgtagtaa atgacactgg cgaattatac aacgtaatca atcaaaagaa atctgaaggt
     7501 tatttagaat cagagctaac aattattagt aaaagtaaat tacacctaaa tgacttacac
     7561 gactctgaaa tctcccttat ctcaacaagt ggaacattta gtgatagaat gactaaactg
     7621 ttaacaggag aagatggtga acatgcagtt ttatcacgct acaacttagc gcctgatgaa
     7681 ttagaaaaat ataaacaact tattttagac gataaaatgt tagttgtagc ggttcgtgac
     7741 aaatcatcac atcaagaagt gcatgaaaat aattcagctt atgaagagat tgatattaca
     7801 cattttgctg aagcatctaa aggtcccaaa gcttagttat cacactgaat tcaaaatgag
     7861 attactgtac tgtctaattt aagatagtac agttttttat accatttaaa agtttatgaa
     7921 cagtctttta cagcaggcga ttatgctata atattattta aattggggag gtgttcttat
     7981 ggaatttaaa accattcaat ctgtcaaaga tccacattac aacgaagcac ttaaacttta
     8041 cgatactaag ttaaatttat ctttaactga agataaacgt atcatcaaac gctcattaga
     8101 aaacaacaaa accgaaaacg attatgtctt tatcgtaggc ttagaaaacg atgttgttgt
     8161 cagtctagca actgcacatt atgaagccac aaccaattca gcatttttaa tttatttatt
     8221 cgcaaaagac ctacccaacc atgatgagtt aatgacgcaa acattacatc aaattgaaca
     8281 tgaactaaac ttattatcaa accttgttca ttctagaagt attaactttt taatgatgga
     8341 agtccctaaa gaacctgcag atttaacaca agataacggc ctcattattg aacaccgtag
     8401 acagttttta tacgaacatc aatttgaaag acaaactgaa attgattaca ttcatccaac
     8461 taatgatggt cacactaaaa aagtggactt atttttaaag aaaaatatta aactgacaaa
     8521 ggatatctac ggtacaagtg ttaaatcaaa ttatattttg aagtatgtat ttgccaatgg
     8581 tatcgcacgt gaaattattt atgctttatt agaagaaatg gatttacgta aaccatttta
     8641 aacaaacttg aaatcactaa caataacgtt taaactatgt atgaagcaag actgacgttt
     8701 cactgcccgt agacaaacgt cagtatacat aaagtatatg cagaaaggac tttaaagtat
     8761 gttaactaaa gaatttgcac aacgcgtaga actaagtgag aagcaagtac gtaaaattgt
     8821 tcaacattta gaagaacgtg gttaccaact aagcaagact gaatatcgtg gtcgtgaagc
     8881 aactgatttc aaagaagaag atatcgagct tttcaaagac attgccgaca aagtaaaaca
     8941 aacaaatagt tatgatctag cgtttgatga attagaaaaa gaaaaagact tcctgcaagt
     9001 cattgtcaaa aacgatgaca aaaacttacc tactaatcaa aatgtcgctc aactagtaga
     9061 agatttacgc ctagaaatcc agaaaatgcg cgaagaacgt cacctacttg gtcaaatgat
     9121 gaatcaagta catcagcaac aacaagaatt aaaagaactt caaaatcaac ttacatctaa
     9181 aatcgattca aatagcgaat ccttaaaagc catccaaaca tcacaagagg ctatccaaga
     9241 agcgcaagcc tctcaagcaa aagcattagc tgaatcaaca aataaagttg aaaagaatgc
     9301 tgttactgaa gataaagctg acagtaaaga ttcgaaggtt gctggagtga atacatcaac
     9361 agacgctaag acggatacta aagcagaaaa tgcaggcgat ggcactgcta ctaaagtaga
     9421 taaagaggat caaatttctg caactgaagc aattgaaaaa gccagcgttg agcagtctaa
     9481 aaatgacaat gcagctgaga cttcaaataa agaagctaca gtagatgcag aagcgcaaca
     9541 tgatgctgaa caacaagtag cagaagccca tgcagaagct tctaaacaag ctacttcgaa
     9601 cgattcattg gaagcgaaag cagaaaatga cagcacagct tcacagtcag aaatgtcaga
     9661 accaaaacct caagaagaga aaaaaggttt cttcgcacgt ttattcaacc tgtaaacttg
     9721 tgatttgaac acaaatttaa tactcaattc atatatagct ttgatttaaa aactgatttt
     9781 tataaaccta tagaactgta atttactttc aaatttaata ctcaattcat aaaacctgaa
     9841 acaagctgat atactccatt caatatctac tttgaagaga gcatatcaaa ctaatttgtt
     9901 tcaggttttt taattattca ttatgattaa tattgaattt tgaataaatt tttatagtat
     9961 gttactttta aatgggatgg cttctgtgta cttctaaata gattcatctt aataagtcca
    10021 ttcaatttaa atgtttggct gtgttagtaa ctgacgaatc gcacaagtgc acactcatat
    10081 tcacatttac tacaaacgcc tttttgctca aacaaaaata tttgtttacg gtagcttttg
    10141 aactaccacc tgcacacacg aacactcaaa agatgctaat tttgattcga cagaattttt
    10201 tacaacgtgt ttgttcgaaa cgtcttttca aggttgatca tttattatct tttcattcaa
    10261 taaatatgag gttagcaatt tttatcaaac cgccatagcc acatctcgat tcaaggataa
    10321 atttgttttc tcatatctta tctctcatga cagtgcacga tatatatctt ttccaaatat
    10381 tcatcacttt tttcctgtta aaatcatttc tattattaat gctttaatga caacttattt
    10441 aatttattgc atttaatgcc ctgcttcatt ttcaaatatt aactttaatc gactagctca
    10501 tcacactatt taccctttcc acctatcact actgaattca aaacgatttg tttacaaatc
    10561 cccttatgcc aacggtcgaa tttcatcatg tttacattta attgcaaatg ccctcttgaa
    10621 atattttaaa attaatatta ttcttagcat actagtcgga attatattca aaaatttgag
    10681 gagtgacgtt catgaatgct tttcttacac tgattaatat tattgtattg gtgattttca
    10741 tcgtaatatt gcacatgatg gctcgcaaac atatatcttt tgcgaaacgt gtgtttacag
    10801 ctttagggat tggtatagta ttcggtgtgt tattgcatct tatttatggc acacattcga
    10861 atgttattac atcaacaagt gactggttca acattgtcgg acaaggttac gttgcattgt
    10921 tgcaaatgat tgttatgcct ttaattttca tttctattgt agctgcattc actaaaattc
    10981 aaatcggaga aaagtttgct aaaattggta gtctcatttt tatcttttta attggcactg
    11041 tcaccattgc tgcgattgtt ggtgttgtgt atgccttggt gtttggttta gatgcatcaa
    11101 caattaacct tggtaacgct gaacaagcac gtggaagcga aattgctaaa caagcgaaag
    11161 atttaacagc acatacgtta ccacaacaaa ttttggaatt attgcctaaa aatccattct
    11221 tagatttcac tggacaacgt gcaacttcca cgattgctgt cgtgatattt gcttcgttta
    11281 taggatttgc ttatttacgc gtagcacgca aacaacctga tcatggtgaa ttgcttaagc
    11341 gtgcgattga cgctatctat tcactagtaa tggcgattgt gacgttcgtc ttacgtttaa
    11401 cgccatacgg tgtattagca attatggcca acacattatc gacaagtgac tttggcgcca
    11461 tttggacttt aggaaaattc ttaatcgcat cgtacgctgc attaattacg atgtatatca
    11521 ttcacttaat tattttaagc ctgttaggta ttagcccaat tcgctatgtt aaaaagactt
    11581 tggaagtatt aatctttgca ttcacatcac gctcaagcgc gggtgcctta ccattgaatg
    11641 tacaaacaca aacaagacgt ctaggcgtgc cagaaggtat tgccaatttc gctgcgacat
    11701 ttggattgtc gattggacaa aatggttgtg caggcattta ccctgccatg cttgcaatta
    11761 tggtcgcacc cgtcgctaat gtagaaattg atttacaatt tattgtaacg ttaatcgctg
    11821 tcgtgattat tagttcattc ggcgttgctg gcgtcggtgg cggggctaca tttgcatcca
    11881 tattggtgtt gtcaacgtta aacttgccag tcgctttagc cggcgtgctc atctccgttg
    11941 aaccattgat tgacatgggt agaacagcat taaacgtgaa cgattcaatg ctagctggta
    12001 caggtaccgc caagttaact aaacattggg ataaagatac tttcgattca aatgataatg
    12061 ctgcattaac ttcacactaa ttcattgcaa gaaatacccg ccccctctag ctatacttat
    12121 cattttaagc tagagggggg cgggttattt tggtatcttg ctatcgctgt attaagcctg
    12181 atttttgcaa ttgttctaac atatctaatc ttgctttgtt tcctaggaac atctcaattt
    12241 gctgcgacca tgtttctttg cgattcccgt ttgtacgttg atcatagtac tcgctgattg
    12301 tctggtcgta atctgccatt tgtgcatact gtgtttcctt atcagcatga tacttattat
    12361 gatggaagac atggtcaaat ggtaagcgtg gcttggctgc accattttcg tcatctgcgg
    12421 gttcccctac tgccatacca aataccggga agacatagtc aggtaagtct aaaatttctc
    12481 gaacgcgttc aacatcattt cttaatgatc ctaaaaagac aatgccatac cccatatctt
    12541 cagcagttac cgcaatattt tcggcaacta atgctgcatc gattgcacct actagcaaac
    12601 cttccgttga accatatgca ttttccatat cagtttcagc atgttgatca actaaatgat
    12661 gacgataata atcaataaca aagacgaata aatagccatt ttctacaaca taaggttgtc
    12721 cagaaacttc tcgtaaattt tctttaatct tctcatcgtc gataccaata attgagtatg
    12781 cttgcaggaa acttgacgtc gaagcgcttt gtccagcttc taccaatttc ttaacaacgt
    12841 cttcacttaa aggtttattc ttaaattttc taacagaatg atgctttttc acaagattat
    12901 atacatgttc tgacacgtta cccactcctc gatactttac aattaattta aaatcttcat
    12961 attgcaattg tacacgtcca aactttttta tagcaattat ttgcttataa gttaataatt
    13021 gaacttttta aaatattgtc tcatacttta aaaatccagt ttctcactca ttaccttcat
    13081 ccatctcgaa caaatattct cacaccatta tcaataaggt tgacttagtt gagaatacaa
    13141 atcttctttt tttacatttt ttacattaaa atcaagatat taaatataga cacaaccttt
    13201 taaatcatca actacaaaat ccattatcaa agcactttat attatctttc tttattttaa
    13261 aagtaatcat tcactttaca aagttatcca gttgactatt ctggatcaaa atgtaataat
    13321 gctaactaag agattagaat tattataatt aagcgcaatc aattgatagt tctaattcat
    13381 acttaaattc ttaggaggaa gatatttatg tcattaatta acaaagaaat cttaccattt
    13441 acagcgcaag ctttcgatcc aaaaaaagat caatttaaag aagttacaca agaagattta
    13501 aaaggttctt ggagcgtagt atgcttctat cctgctgact tctcattcgt ttgtccaact
    13561 gaattagaag acttacaaaa ccaatatgaa gaattacaaa aattaggcgt aaatgtattc
    13621 tcagtatcaa ctgatactca cttcgtacac aaagcatggc atgaccattc agatgcaatt
    13681 agcaaaatca cttacactat gattggtgac ccatcacaaa caatcactcg taattttgat
    13741 gtattagatg aagctactgg tttagctcaa cgtggtacat tcattatcga cccagacggt
    13801 gttgtacaag catctgaaat taacgctgac ggaattggcc gtgacgctag tacattagct
    13861 cacaaaatca aagcagctca atatgttcgt aaaaaccctg gcgaagtatg cccagctaaa
    13921 tgggaagaag gcgctaaaac attgcaacct ggtttagatt tagtaggtaa aatctaagga
    13981 ggcattacaa ccatgcttaa tgctgattta aaacaacaac ttaaacaact attagaacta
    14041 atggagggca acgttgaatt cgttgccagc cttggttcag atgataaatc caaagaactt
    14101 aaagatttgt tgacagaaat tactgatatg tcacctagac tatctctttc tgaaaaatct
    14161 ttaaaacgta caccaagttt ctcagtcaat cgtcctggcg aagaaacagg tgtaacattt
    14221 gcaggtattc cattaggtca cgagtttaac tcacttgttt tagcaatttt acaggttagt
    14281 ggtcgtgcac ctaaagaaaa acagtcaatc attgaccaaa ttaaaaaatt agaaggttca
    14341 ttccattttg aaacattcat tagtttaacg tgtcaaaaat gtcctgatgt cgttcaagca
    14401 cttaacttaa tgagtgtgat caaccctaac atcacgcatt ctatgattga tggtgcagtg
    14461 ttccgtgaag aatctgaaaa catcatggca gtccctgctg tctttttaaa tggcgaagaa
    14521 tttggcaatg gtcgtatgac aatccaagat attctttcga aactaggcag tacggcagat
    14581 gcatctgagt ttgaaaataa agaaccttat gatgtcttaa tcgttggtgg tggtcctgct
    14641 agtggtagtg cagcgattta cacagcacgt aaaggtttac gtactggtat agttgctgat
    14701 cgtatcggtg gccaagttaa tgatactgct ggtattgaga acttcattac tgttaaagaa
    14761 acaactggtt ctgaattttc ttctaactta gcagcgcaca ttgatcaata tgacattgat
    14821 gcaatgacag gtatacgtgc tacagatatc gaaaagactg acgaagcaat taaagttacg
    14881 ttagaaaacg gtgctgtctt agaaagtaaa acagtcatta ttgctactgg tgcaggttgg
    14941 cgtaagctaa acattccagg tgaagagcaa ttgattaata aaggtgttgc attctgccct
    15001 cactgtgacg gacctctatt tgaaaataaa gacgtagcag ttatcggtgg cggtaactct
    15061 ggggttgaag cagcaattga ccttgctggt atcgttaatc atgttacatt attcgaattc
    15121 gctagcgaat taaaagcaga caacgtgtta caagatcgtt tacgttcttt atcaaatgtt
    15181 gatatcaaaa caaatgccaa aactactgaa gttgtcggag aagaccatgt tacaggtata
    15241 cgttacgaag acatgaacac cggcgaagaa catctactta acttagatgg tatctttgtt
    15301 caaattggtt tacttccaaa cacatcatgg ttaaacgatg ctgttgaatt aaacgaacgt
    15361 ggtgaaattg tgattgatcg taacaataat acgaatgttc ctggaatatt tgctgctggc
    15421 gatgtcacag atcagaagaa caaacaaatt atcatttcaa tgggcgctgg tgcaaatgca
    15481 gcattaaatg cctttgacta tattatcaga aactaaagtt tcattcattt cactgacagt
    15541 atcttacatt tgttaagatg ctgttatttt tttatcccga cataaaatca tgttatgata
    15601 aacttaatat atacaacgag atgtgcgata atgcttaaag tatgtacctc tcgcaaacaa
    15661 atcaatatga aaaatgatat tgagaaagaa aagtatgtga acgaaattat tatgctttta
    15721 cgtacgatac ctacgtctaa attagtacaa ccaattatgc agttatttat agataaagct
    15781 atccttaaca acgtccaaaa tgatgcatta ttgatagaaa aagcaactca tgcacgtaat
    15841 catttacgtc tatggtcatt gcaaggtcat cgtgacattc caaactaatt caatgttact
    15901 ctattaaaca tgaatacata ttttttcaat taaagataaa ctaaggagtt atattacatt
    15961 atgaaaaaga gattactact aagtacattt ttagcatcga cattaattct tacaggttgc
    16021 gcgtccgatc aatctgataa cgaagatcat catacaagta ctggcatcca tgcacctaaa
    16081 agcgctaaaa aattagaaac aaaagatatc tttaactcag ataagaaaaa tagtgatatt
    16141 agtgatgcag agatgaagca agctatagaa aagtatttgt ctgtaaatag cgatatactt
    16201 gataacaaat atattatgca acataaacta gataaacaaa ttgacagtca aacaaaagtg
    16261 actgaaaaac aagcagaaac acttagccac ttatctaact tagctgttaa aaacgatttg
    16321 catttcaaga aatttgtcac tgaaaacaat attcctaaag aatataagaa acccgttgaa
    16381 ttaatgatga attacttcaa agctttaaat agtacaatcg ccaatgttga tgaagatatt
    16441 gaaaagctta gctatcaacc gcaaaataaa atcaatgttg tcgacgtgcc aacaaaatat
    16501 gccggtgatg ttaataaaaa gcaacaagat aaaattaaag atttcttaaa atctaaaggt
    16561 atcaagagtg atgttatcga taaataactg aaagccatca ttgcgaaatg taacacatca
    16621 ttacatgagt tttaattcat gacaattctt tatctttaaa agtgactaat caatttagct
    16681 aaagtcatca tttgggcatc aagcagcata ttactgtttg atgccttttt aattgcctta
    16741 aattctctat ctatgccaaa attcccatta gttgacgcat ttgactatat acctaacata
    16801 ttccatactt atattgactc tataaacaaa ggatgatcat gatgaaagtt cgaattactt
    16861 ttattatttt agcaatttta tcgacgattg tttgcttgtt cttagcagca atgcatccaa
    16921 caggaccaaa cacagtaact tttgaacagc catatttatt caccctcaac atcattataa
    16981 tggtattagt cgcattacct tcacttgttt tagctatata tgattatatg agttttagaa
    17041 tttcttctgc tattttacaa tttctagggg ctatctcttg gttcttttta tcattgatat
    17101 tatcgctcac acaatttaca ccttttacat tagcgtcatt tataacttca attattttgt
    17161 tcacaagcac aattatcaca ttagccattg gtggtaagtc tgttgaaaag aatgattccc
    17221 cttaaattcc aaatgaaaaa aaggttctga aggccgctat aaaacacagt ttttcagaac
    17281 ctctatactt ctattcaatg atatatggtt tgcaattttc tacctttaaa tccacagctt
    17341 ctgcccttga aactttgtta aaataaacca tcaaacaacg aatgacaact tgatgtgcaa
    17401 caatgacaat atcatctttt tgtgtatctt cattgacaac atgattcata aaatgttcta
    17461 cgcgttgata tacatcttca taactttctc cttcaggcgc tttttgtgaa aaactatgac
    17521 gaaagtcttt aaagtttgga tcattgaaat atttatcata tttcggattc gcactgattt
    17581 catctttata ttcaccctca aatacgccaa gtgaacgttc tcttaataga ggggtagtcg
    17641 ttgatgcaat gtcatatgga aaaatatgtt caaacgtttg ctgtgttctt aataagtctg
    17701 aaacatatac atgtttaatc tgtttctctt taaaataatc acataaatcg tctgcacttt
    17761 ttgtgccagt atccgttaac ggcacatcta attgtccaca aaaataagat cgaaaatgtt
    17821 tattatcata attcgatttt gattcgccat gtctaactaa at
//
LOCUS       DAKTLH010000044        17380 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.044, whole genome shotgun sequence.
ACCESSION   DAKTLH010000044 DAKTLH010000000
VERSION     DAKTLH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17380
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.044"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            449..562
                     /locus_tag="O0394_001245"
     CDS             449..562
                     /locus_tag="O0394_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499330.1"
                     /translation="MNGILIKCLNIGGQYYLQTRMLSNVDNAEKIISRNSI"
     gene            944..1204
                     /locus_tag="O0394_001246"
     CDS             944..1204
                     /locus_tag="O0394_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499331.1"
                     /translation="MIIMRGYNGRYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVLEKCITLYNVCSRCKRALILSEHSKKNIFNIKKAV"
     gene            1214..1711
                     /locus_tag="O0394_001247"
     CDS             1214..1711
                     /locus_tag="O0394_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCY0499332.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(1819..1911)
                     /locus_tag="O0394_001248"
     CDS             complement(1819..1911)
                     /locus_tag="O0394_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499333.1"
                     /translation="MSVINAYSNASLLFFIYLLFIMYQISLSVK"
     gene            2106..3986
                     /locus_tag="O0394_001249"
     CDS             2106..3986
                     /locus_tag="O0394_001249"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HCY0499334.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDVLLENLGAERICKRVDCDIDYEED
                     AEKWMADIINIIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLIFMLGWDPQSPVPINDHGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESKRF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRD
                     VY"
     gene            4049..4654
                     /locus_tag="O0394_001250"
     CDS             4049..4654
                     /locus_tag="O0394_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HCY0499335.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGVVASRRAQTLNQYVEHMTVISPTI
                     TEKLQNMVDNGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKKALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKVLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVR"
     gene            4730..4825
                     /locus_tag="O0394_001251"
     CDS             4730..4825
                     /locus_tag="O0394_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499336.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKDT"
     gene            5147..6517
                     /locus_tag="O0394_001252"
     CDS             5147..6517
                     /locus_tag="O0394_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HCY0499337.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            6764..8614
                     /gene="nrdD"
                     /locus_tag="O0394_001253"
     CDS             6764..8614
                     /gene="nrdD"
                     /locus_tag="O0394_001253"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCY0499338.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            8611..9147
                     /gene="nrdG"
                     /locus_tag="O0394_001254"
     CDS             8611..9147
                     /gene="nrdG"
                     /locus_tag="O0394_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCY0499339.1"
                     /translation="MILLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            9247..9357
                     /locus_tag="O0394_001255"
     CDS             9247..9357
                     /locus_tag="O0394_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499340.1"
                     /translation="MIEYDLTIILIPILKVAYPFFKKVEREKWYFSGKEV"
     gene            complement(9373..9534)
                     /locus_tag="O0394_001256"
     CDS             complement(9373..9534)
                     /locus_tag="O0394_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499341.1"
                     /translation="MAIIEYLYNFSRNKQNLNSAKITVLSYDLTNLTNFTRMAFKNIY
                     TLLTNIYLL"
     gene            9666..11288
                     /locus_tag="O0394_001257"
     CDS             9666..11288
                     /locus_tag="O0394_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCY0499342.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLIDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            11552..12238
                     /locus_tag="O0394_001258"
     CDS             11552..12238
                     /locus_tag="O0394_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0499343.1"
                     /translation="MTNQFKNKQSKLHDSLESITKNLYATPTSELPFDNRFLFKSFIL
                     KRETGNIVIYHSGHLGDSQQDIASLGGVSKVLMNHDHESIGGSNQVEAPYFIHENDVA
                     ALKHKISVQKQFSNRVMLDKDLEVIPAPGHTPGTTLFLWDDGHHRYLFTGDFICFEGK
                     RWRTVILGSSDREKSIQSLEMVKELDFDVLVPWVTIKDEPLVYFVENEYEKREQIQNI
                     IDRVREGENS"
     gene            complement(12426..12989)
                     /locus_tag="O0394_001259"
     CDS             complement(12426..12989)
                     /locus_tag="O0394_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HCY0499344.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINSLLESSDLTEAETQEAQKIKSQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(13078..13173)
                     /locus_tag="O0394_001260"
     CDS             complement(13078..13173)
                     /locus_tag="O0394_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499345.1"
                     /translation="MKNVSIYKTTQELSFVSSLDFYLTKHLTLML"
     gene            13242..14732
                     /gene="betB"
                     /locus_tag="O0394_001261"
     CDS             13242..14732
                     /gene="betB"
                     /locus_tag="O0394_001261"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCY0499346.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            14993..16702
                     /gene="betA"
                     /locus_tag="O0394_001262"
     CDS             14993..16702
                     /gene="betA"
                     /locus_tag="O0394_001262"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCY0499347.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            16980..17192
                     /locus_tag="O0394_001263"
     CDS             16980..17192
                     /locus_tag="O0394_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCY0499348.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
BASE COUNT         6092 a         2496 c         3331 g         5461 t
ORIGIN      
        1 catattttat gcaacacata aaatgggtaa ctaacatcac gaaatcattg tatttgttgt
       61 tacactgatg aagcgatgtc acaaagaaat gtcgttatca aacgaagccc gaaatacata
      121 ctgatactcg aaataatgta acgataaata acaatgataa cgattgatta ctacgcaaaa
      181 atatatgtaa attgacgatt taaatgcaat tcgtatattt tgccaatgat ttacacatta
      241 aaatgtttga aaattgtcac aattcagtct aatttaaaag gaagttaatt acaaaattaa
      301 aaaatcatgc atcgtgtcat ttagatattg aaaaagatga gtcaaatttg tttcatatat
      361 tttccaataa caaattatta tatctaaata tacaatcaga aatatatgaa tagaaatatc
      421 aataagaaaa ataatatgat taaaaatgat gaatggcata cttataaagt gtctaaatat
      481 tggcggtcaa tattacttac aaacacgaat gttaagtaat gtagacaatg ctgaaaaaat
      541 aataagtaga aactctatat agaatgttcg tttttatttt taataattga ataatcatta
      601 ttgatgattt aatatgtatt cggtcgcaca cgatacaaag tgacttgttt taatgatatg
      661 caataatatg acgtttatcg tcgcgtgtaa caatgtatga gtgtgtcagt ttaattttaa
      721 aagggttata aggatattta ataagataaa ggagggctta ataaacaatc acatgtgaag
      781 tttttaagga ttatatgagt tgggcagctc tttaatccat agttataacg aattttaaat
      841 tgttagatct aacgtatggt atcagtatgt atgagtttaa cttaagtatt aatctatagt
      901 ttaaagagcg gtctaattgt ctttttaaaa atagaaaata aggatgatta taatgagggg
      961 ttataacgga agatattgta gctggactaa tacagctata caatgtgatt attttaataa
     1021 agtaagacag gtgaaaaaag acggtaaatc ggataggttg agcaacaaca taaactcgaa
     1081 cattatttat cgtgtgctcg aaaaatgtat cacgctatat aatgtgtgtt cacggtgtaa
     1141 acgcgcactt attttatcag aacattctaa aaagaacata ttcaatataa aaaaggctgt
     1201 gtaggggtgt cttatgaata atagaaatgt gtatgatatc gaagtaagtg attataaagg
     1261 cttaacttat aaattagaag catttagagg taaagtgatt ttagttgtta atactgcaac
     1321 agaatgtata tatagcgaac aattgaaaaa actagagact ttatttcaaa aatataagga
     1381 tcgtgggttt gtagtgttga gttttccgaa taataatttt gacaatcgac aaccaggttc
     1441 taatgaagaa atcttgaaaa ttagtcgtga gaaatttggt gtaacatttc cagtgttagc
     1501 taaaatatct gtgaacggga acgatgaaca tccgttattt acgcatttaa agaatgaaca
     1561 accaggaatc tttgggtccc caataaaatg gaatttcaca aaatttataa tcgatcgaca
     1621 aggaaatgtt gtaaatcgat tcttgccaat ggaagatcca ttggatatat cgacaaatat
     1681 agaaatatta ttgcaagaat cttcatctta aatttaaatt tgagcgctta gtatgcaaat
     1741 aacacaagag cctcttaatg atgtactaat agtatgttat taggaggcat tttatattta
     1801 gaaaatcagt aatttgacct attttactga aaggctgatt tggtacataa taaatagtaa
     1861 ataaataaaa aataacaacg atgcattact gtatgcatta ataactgaca cgacataatc
     1921 aaagttttgt ctgaattact gataatgtca ttcaagcaac atgaaggttg taaatatttg
     1981 caagtataac tatcagacac aactcaagtt tgttttaatt tataccatac atattaaaaa
     2041 attgactaaa ctatcgataa aaaaagagtc aatcatctat ttcaagttta ttgaggtgaa
     2101 aacatttgaa attaaataca tctaatagtc catttacaga aaaacaagtc acagagatta
     2161 acaatctgct gcaaacatta acagagagcc aacaacagtg gcttagtggt tttttactag
     2221 ctaatagtaa cgatacgaca agtgatagta atcaacaaca attagagaca gaagtgtggc
     2281 aacaatcaca aatatcagaa gaacaagcaa cttcaacaac gtatatgtta caaaataaag
     2341 agccacatat cgaagctaat cagcggcatg ttacagtgct atatggttct gaatcaggta
     2401 atgccatgcg tttagctgaa attttttcag aacgtttaag tgatatcgga catcaagttg
     2461 ttttgatgtc aatggatgaa tatgatacga caaacatcgc gcagttagaa gatttattta
     2521 ttattacgtc tactcatggt gaaggagaac cgcctgataa tgcatgggat ttctttgaat
     2581 ttttagaaga cgataacgca cctaatttaa atcatgtgag atattcagta ctagctttag
     2641 gtgatcaaac atatgaattt ttctgtcaag ccggtaaaga tgtagatgtt ttactagaaa
     2701 atctaggcgc tgagcgtata tgtaagcgtg tagattgtga tattgattat gaagaagacg
     2761 cagaaaagtg gatggcagac atcattaata ttattgatac cacatcagaa ggtattcaaa
     2821 gtgaatcggt gataagtgaa tcaattaagt ctgccaaaga aaagaaatat tctaaatcaa
     2881 atccatacca agcagaagta ttagcgaata tcaatttaaa tggtaccgat tcaaataaag
     2941 aaacacgaca tatagaattt ttacttgatg attttagtga atcatatgaa ccaggagatt
     3001 gtatagtagc attaccgcaa aacgaccctg aattggttga aaaactaata ttcatgttag
     3061 gttgggatcc gcaatctccg gtgccaatta atgatcatgg tgatacagtt cctattgttg
     3121 aagcactaac atcacatttt gaatttacta aattaacatt gccattattg aaaaatgcag
     3181 atatctattt tgacaatgaa gaattatctg aacgtattca agatgagtca tgggcgcgtg
     3241 aatatgttat aaatcgggac tttatagatt taataacaga ttttccaact atagaattac
     3301 aacctgagaa tatgtatcaa atccttagaa aattaccacc aagagagtat tcgatttcta
     3361 gtagttttat ggcaacgcca gatgaagtgc atattaccgt tggtacggtt cgttatcaag
     3421 cacatggacg tgagagaaaa ggtgtatgct cggttcattt tgctgagcga attaaaccag
     3481 gcgatatagt accaatttat ttgaagaaaa atccgaactt caaatttccg atgaagcaag
     3541 atataccggt tattatgatt ggaccaggta ctggaattgc tccttttaga gcatatttac
     3601 aagaacgtga agaacttggt atgactggaa aaacatggtt gttctttggt gatcaacacc
     3661 gtagttctga ctttttatat gaagaagaaa tagaagaatg gcttgaaaat ggaaacttaa
     3721 cacgcgtaga tttagcattt tcaagagacc aagaacacaa agaatatgta cagcatcgta
     3781 taatggaaga aagtaaacgt ttcaatgaat ggattgagca aggcgcagca atctatattt
     3841 gtggcgatga aaaatgtatg gcgaaagatg tccatcaagc cattaaagat gtattggtaa
     3901 aagaacgtca tatttctcaa gaagaagcag agttattatt gcgacaaatg aaacaacaac
     3961 aacgctatca acgtgatgtt tattagcgat tggtgttaaa tattttaagg tgtaatgatg
     4021 taaaaagata taaaggatgt tgctcaacat gaatatgcca ttaatgatag atttaacaaa
     4081 taaaaatgtc gtcatagttg gtggaggcgt cgttgcaagt cgtcgggcac aaacattaaa
     4141 tcaatacgtt gaacatatga cggtcatcag tccgacaatc actgaaaaac ttcaaaatat
     4201 ggtagataac ggtgtcgtca tatggaaaga aaaagaattt gaaccaagcg atattgtaga
     4261 cgcgtatcta gttattgcag caaccaatga gccacgtgtc aatgaagcgg taaaaaaagc
     4321 cttacctgag catgcccttt ttaataatgt tggagatgca tcaaatggca atgttgtatt
     4381 tccaagtgca ctacaccgcg acaagctaac tatcagtgta tcaactgatg gtgcgagtcc
     4441 taagttgaca aaatcaatta tggcagagct tgaggcgtta tatccaccat catacagttc
     4501 gtatatcgac tttttatata cttgccgaca gaaaataaaa gtacttgata taacatataa
     4561 cgaaaagcaa cagttactgt cacaaattgt gtcacaagaa tatttaaatc atgacaaaca
     4621 agctcaattt ttagcgtggt tggatgtaag ataataatag cggaccgtct aaccgtctaa
     4681 ggtaagtctt cttattttaa ctttaacgct taatcattga aattaagaca tgggcggctt
     4741 tgtgaatagt ctaataatga aggatttaag cgataatgat atgcgtttta aatatgaata
     4801 ttacaataga gaaaaagata cgtagaacaa acttaataaa ataggtggat aaattgaaat
     4861 ctggttgaag tcgttactat catagcgacc tttagccaga ttttttgtgc aatagaaagc
     4921 aataataaaa atgatagatc aaaatgaaat acaggacagg atatacaagg attagtcatg
     4981 ccatgttatc aagtaggaaa atcaaacttc actattgata gttacgcaaa aaagattttt
     5041 ttgataaaat gagataactt aaatataaaa aattatatta attataatat ttaagttaaa
     5101 gagggggatt atgtaaattg tattaaaagt ggagggagaa aataatatga atagtgataa
     5161 tatgtggtta acagtaatgg ggctcattat tattatttca attgtaggtt tactcattgc
     5221 caaaaagata aatccagttg taggtatgac aatcatacct tgcttagggg caatgatttt
     5281 aggatatagt gtgacagatt tggttggatt ttttgctaaa gggttagatc aagtcatcaa
     5341 cgttgttatt atgtttatct ttgccattat tttctttggc atcatgaacg atagtggttt
     5401 attcaagccg cttgtcaaac gcttaatatt aatgacacga ggcaatgtcg tcattgtctg
     5461 tgcaatgaca gctttaattg gcacaatagc ccaattagat ggggccggtg cggtaacatt
     5521 tttgctttct attcctgcat tattaccttt atataaagcg ttaaatatga ataaatattt
     5581 attgatttta ctattagcat taagcgcggc gattatgaac atggtacctt ggggaggtcc
     5641 aatggctcgt gtagctgcag tgttaaaagc caaaagtgtc aatgaattat ggtatggatt
     5701 aatacctatt caaataatag gtttcattct tgttatgttg tttgcggtat atcttggatt
     5761 taaagaacag aaacgtatca aaaaagcaat agagagaaat gaattaccgc aaacacaaga
     5821 tatagatgta cataaattag ttgaagtata tgaacgagat caagatgtaa ggtttcctgt
     5881 aaaaggacgt gcaagaacaa aatcatggat aaaatgggtg aatacagctt taactttagc
     5941 tgttattcta tcgatgttaa taaatattgc gccacctgaa tttgcattca tgataggtgt
     6001 ttcgttggca cttgttatta attttaaatc agtggatgaa caaatggaac gattaagagc
     6061 gcatgcgccg aatgcattaa tgatggctgc agtgattatt gcagcaggta tgtttttagg
     6121 tgtactaaat gaaaccggta tgcttaaagc gattgcgacc aatttaatca aagtgattcc
     6181 tgcagaagta ggaccatact tgcatattat tgtaggttta cttggcgtac cattagattt
     6241 actaactagc acagacgctt attattttgc tgtgttaccg attgtcgaac aaacagcagg
     6301 gcaatttggt gtaccgtctg tatcaacagc ttattcaatg gtcataggga atattatagg
     6361 tacatttgtc agcccatttt caccagcctt atggttggca attggtttag cagaggcaaa
     6421 catgggcacg tatattaagt atgcattctt ttggatttgg ggattcgcta tcgttatgtt
     6481 agtaattgca atgttgatgg gcattgtgac gatttaagta tgaaaaaata gaaactatgg
     6541 tcacgttgca aaatgaaata atagttgcat aaacatgtcg aaatgacgga cgaatcttta
     6601 aacaatttta aaaattaatg aaataattgt gtagaaatat gaatttcact aaatgttaat
     6661 aactttgtga cgttttagtt aacagactaa taaaaatttg aaaatactat atatagtggt
     6721 ataacgtaat gagtagacac aatatatagg aagaaggggt aaaatgaatc aaatcgaaga
     6781 agcattaacg ggtttgattt ctaaagatcc tgctattgtt aacgaaaatg ctaacaaaga
     6841 tagtgataca ttttcaacaa tgagagattt aacagcaggt atcgtttcta aatcttacgc
     6901 attaaatcat ttattaccaa agcacgttgc agatgcacat caaagagggg acatacattt
     6961 tcacgactta gattatcatc cattccaacc gttaactaac tgttgtttaa tagatgctaa
     7021 aaatatgcta cataatggat ttgaaatagg caacgcgaat gtaacttcac caaaatcaat
     7081 acaaactgca tcagcgcagc ttgtacaaat tatagccaat gtttctagca gtcaatatgg
     7141 tggctgtacg gttgaccgcg ttgacgaatt acttagtaca tatgcacgac ataatgaaga
     7201 acaacatagg aatatcgcaa agcaatttgt caaagaatct gaaattgatc gttatgttga
     7261 tcaacaagtc actaaagaca tcaatgatgc gattgaaagt ttagaatatg aaattaatac
     7321 cttatataca tctaatggac agacaccttt tgtaacatta ggattcggct taggtacaga
     7381 tcatttaagt cgcaaaattc aacaagctat cttaaatact cgtatcaaag gcttaggaaa
     7441 agaccgcacg acagcgattt tcccgaaact tgtattttca attaaaaaag gaaccaactt
     7501 tagtccgcaa gatccgaact atgacattaa acaactagca ttaaagtgtt caacgaaacg
     7561 tatgtatcca gatattttaa attatgacaa actcgtagaa atattaggtg atttcaaagc
     7621 gccaatgggt tgtcgttcat ttttaccaag ttggaaagat gcggaaggtc attttgaaaa
     7681 taatggtcgt tgtaatcttg gtgttgttac acttaattta cctagaatgg cattagaatc
     7741 tgccggtaat atgacgaaat tctgggaaat cttttatgaa cgtatcgatg tgttacatga
     7801 tgcattactt tatcgtataa atcgtttgaa agatgctgta ccgaataacg caccgatttt
     7861 atataaaagt ggcgcattta actataaatt aaaagaaaca gatgatgttg ctgagttatt
     7921 taaaaataaa cgtgcaacga tttcaatggg ctatataggg ttgtatgaaa cagctactgt
     7981 tttctatggt ccagactggg aaacatctca agaagcaaaa gcatttacgc ttgaaattct
     8041 taaagaaatg aaacgttatc aaacgaaatg gacagaatta tatgacattt ggttcagtat
     8101 ttacagtacg ccgagtgaat cgctaacgga tcgtttttgt cgtttagacc aagagagatt
     8161 tggagatatt aaagacatta cagataaagg atattatcaa aactctttcc attatgatgt
     8221 acgtaaagat gttacacctt ttgaaaagtt agattttgaa aaagattatc cttattatgc
     8281 gagtggtggt ttcattcact attgtgagta tccgaaattg caacacaatt tgaaagcact
     8341 agaagcggta tgggactact cttatgacaa agttggttac ttaggtacaa atattccgat
     8401 tgatcattgt tatgaatgtg attacgatgg agattttgaa gcaactgaaa aaggatttaa
     8461 atgcccgaac tgtggcaatg ataatcctaa aacagttgat gtcgttaaac gaacatgtgg
     8521 ttacctaggc aatccagttc aacgtccagt aattaaaggc cgtcataaag aaatttgcgc
     8581 acgagtaaaa catatgaaag cgcctaaaga atgatacttt tagacattaa acaaggacaa
     8641 ggttatattg ctaaaataga atcaaatagc tttgttgacg gtgaaggagt aagatgcagt
     8701 gtttatgtat caggatgtcc atttaattgt gttggatgtt ataacaaagc ctcacaaaag
     8761 ttcagatatg gcgagaaata cactgatgaa atattagcag aaatattaga tgattgcgat
     8821 catgattata tatctgggct aagtctatta ggtggcgaac cattttgtaa tttggatatt
     8881 acattaaatc ttgtcaaagc atttcgagca cgttttggaa atacaaagac aatttgggta
     8941 tggactggat ttttatatga atatttagca aatgattgta cagaacgtcg agagttatta
     9001 tcatacattg acgttttagt agatggtcta tttatacaac acttattcaa acctgattta
     9061 ccatataaag gttctttaaa tcaacgcatt atagatgtac aacaatcact ctcgcatgcg
     9121 cgtatgattg aatatatagt tagttgaata tgtattagaa gtcaaggtaa cattcgttgc
     9181 cttggcttct ttttaggtta ggtacataat tgaaagttaa taaaagcaat tctttataaa
     9241 aatatattga tagaatatga cctaacaatc attttgatac caatactaaa agttgcatat
     9301 ccgtttttta aaaaagttga aagagaaaag tggtatttta gtgggaagga agtctaactt
     9361 tttggtagcg ttttacaata aataaatatt cgttaataac gtataaatat tcttaaatgc
     9421 cattctagta aaatttgtta aattcgttaa atcgtaactt aacactgtta ttttagcgct
     9481 attaaggttt tgtttattac gggaaaaatt atataaatat tcaataattg ccaagtttca
     9541 aattgtatga aatttgcatt attattaaat gttagttatt gtcaattttg tgaatcaata
     9601 taattattac attttgagat aaatcgaaac aggattcata aaattaataa ttagggggag
     9661 cacaattgaa aaaagagaaa gttatggact ggacgacctt tatagggaca gtagctgtac
     9721 ttctttttgc agttatacct atgatggctt ttccaaaagc aagtgaagat atcatcactg
     9781 gtattaatag tgccatttct gattcaattg gttcgatata tttatttatg gggctggcga
     9841 ttttttgctt tgtaatgtat atcgcatttg gtaaatatgg taatgtcaca cttggtaaag
     9901 caagtgataa accagaattt aatacattta catgggcggc aatgctgttt tgtgcaggca
     9961 taggctctga tattttatac tggggcgtta ttgaatgggc tttttactat caagttccac
    10021 caaatggcgc gaaaagtatg agtgatgaag cactccaata tgcgacgcaa tatggtatgt
    10081 tccactgggg gccaattgct tgggctattt atgttctacc agcattacca attggttatt
    10141 tagtatttgt taaaaaacaa ccggtgtata aaattagtca agcttgtcgt ccgattttaa
    10201 aaggtcaaac agataaattt gtaggtaaag ttgtagatat cttatttatc tttggattgc
    10261 taggtggtgc ggcaacatca ctagcgttag gtgtgccatt aatttctgca ggcatagaaa
    10321 gattaactgg tttagatggt aaaaatatga ttttacgttc ggccatttta ttaacaatca
    10381 cggttatatt tgccattagt tcatatacag gattgaaaaa aggtattcaa aagttaagtg
    10441 atatcaacgt ttggctatcc tttgtacttt tagcctttat atttattatt ggaccgactg
    10501 tttttattat ggaaacgaca gtgacagggt tcggaaatat gttgatagat ttctttcata
    10561 tggcaacatg gttagaacca ttcggtggta ttaaaggtcg aaaagaaacg aatttcccac
    10621 aagactggac aatattctac tggtcatggt ggttagtata tgcgccattt atcggtttat
    10681 ttatcgctag aatttcaaaa ggtcgacgcc ttaaagaagt cgtgctagga acaattattt
    10741 atggaacgct tggatgcgta ttattctttg gtatttttgg taactatgct gtgtatttac
    10801 aaatttctgg acagtttaat gtaacacaat atttaaatac acatggtaca gaggcaacca
    10861 ttattgaagt ggtgcatcat ttaccattcc catcattgat gattgtacta ttcttagtat
    10921 ctgctttctt attcttagca acaacatttg attcgggttc atatatttta gcggcagcat
    10981 ctcagaaaaa agtggtaggc gaaccattac gtgccaatcg tttattctgg gcatttgcat
    11041 tgtgcttatt gccattttca ttgatgctag ttggtggtga acgtgcatta gaagtattga
    11101 aaactgcttc aatactggca agtgtgccat taattgttat ttttattttc atgatgatat
    11161 catttttaat cattttaggg cgcgatagaa ttaaacttga aacgcgtgct gaaaaattaa
    11221 aagaagttga acgtcgttca ttgcgaatcg ttcaagtatc agaagaagaa caagacgata
    11281 atttataatt caaagcgggt ctgggacgac gaaatgaatt ttgtgaaaat atcatttctg
    11341 ttccattccc ctttttttag tagcattgta ggatgaactt ttaggttttc attaatgttg
    11401 tactaaaaga tttaattttt tagtgctcca agtacttatt tattgtatga agcatattct
    11461 aaatcgaagt ttgaaagact ctcattgatt attaaattaa ataaagggta ctgcgtatgt
    11521 acaattcaaa ttaatcgaag gatgaaataa aatgactaat caatttaaaa ataaacagtc
    11581 caaattacat gacagtttag aatccatcac aaaaaactta tatgcgacac ctacttcgga
    11641 actgcctttt gataatcgat ttctatttaa atcttttatt ttaaaacgtg aaacaggtaa
    11701 tattgttatc tatcattccg gacacttagg tgactcccaa caagacattg catcattagg
    11761 tggtgtttca aaagtattga tgaatcatga tcatgaatct ataggaggtt ctaatcaagt
    11821 tgaagcccct tactttatac atgaaaatga tgtggctgca ctgaaacata agatttctgt
    11881 tcaaaaacaa tttagtaatc gtgtaatgtt ggataaggat ttagaagtta ttcccgcgcc
    11941 tggacataca ccagggacga cactattttt atgggatgat ggtcatcacc gttacttatt
    12001 tactggagat tttatatgtt ttgaagggaa gagatggcgt acagttatat taggttcaag
    12061 tgatagagaa aaatctattc aaagtttaga gatggttaaa gaattagatt ttgatgtact
    12121 tgtaccttgg gttactatca aagatgaacc gttagtttat tttgtagaaa atgaatatga
    12181 aaaacgtgaa caaatacaaa atattattga tagagtacgt gagggcgaga atagctaatt
    12241 gaaatatatt ggcgaagcaa tgtaacgaat ctaagaaagc cctagaaaat acctccataa
    12301 ttgattgtca tataaaacaa aaacggtaat ttctatttat tgagatagaa attaccgttt
    12361 atttcgtgga cctattgcat tgtttttatc atgcataatc atcattgtcg ttgtttgagt
    12421 caattttaat tttcagaatc agaaggctgt tctggaattg ggaaatattt gaaaatttca
    12481 ccgctttcaa tcgcttcggt taactgttct aaccattcgt aataaacatg tgtatgatca
    12541 agctgagatt taattttttg tgcctcttgt gtttcagctt cagttaaatc actgctttca
    12601 agtaatgaat tgataatagc ttgagcatct tttactgctt cgacattgat gtcaatttca
    12661 cgctggaatt ttttagtgaa aaagtttcgg aaaaagatga aaaagtcttt ctcggcgata
    12721 aaatgttgtt tgcggcttcc tctcgtaaat tgttgtttaa caatatcaaa ttcctgcaat
    12781 ttcttaacgc cagcactcat acttggtttg ctcatttgca attgatgacg catttcatca
    12841 agcgtcatac tgccttcaaa caccattgtg ccatataagt ttcctacact tctattagtg
    12901 ccatacaaat ccattgtctg tccaattgaa ttaattacaa tatcttttgc ttgttctaat
    12961 tgttgctgtt tgttctgaga acgagtcatc attgcacctc cgtacatcat tttggtcacg
    13021 ttaaaataaa tactaataca ttataaaacc ttttctaaaa aaagacatta aaaatattta
    13081 aagcattaaa gttaaatgtt tcgttaaata aaaatctaac gaacttacaa aacttaattc
    13141 ttgagttgtt ttgtaaattg acacattttt catttctatg ctaacataag tttgtaaaat
    13201 tcgttaaata aaaatttaac aaacttaacg gaggttgttg aatggaactt ttaaaacatt
    13261 tatctcagcg tcaatatatt gatggtgagt gggttgaaag cgcgaataaa aatacaagag
    13321 atattatcaa tccttacaat caagaagtga tatttacggt ttctgaaggg acaaaagagg
    13381 atgcagaacg tgcaatctta gctgcaagac gtgcgtttga gtctggtgaa tggtcgcaag
    13441 aaactgctga aacaagaggt aaaaaagtac gtgccatcgc ggacaagatt aaagaacatc
    13501 gcgaagcgtt agcacgatta gaaacattag atactggaaa aacgttagaa gaatcatatg
    13561 cagatatgga tgatattcat aatgtgttta tgtattttgc tggattagca gataaagacg
    13621 gtggcgaaat gattgattca ccaattccag atacagaaag caaaattgtt aaagaaccag
    13681 taggtgtagt tacacaaatt acaccttgga attatccgtt attacaagca tcatggaaaa
    13741 ttgcgccagc gcttgctacg ggttgttcac tagttatgaa accaagtgaa attacaccat
    13801 taacaacaat acgtgttttt gaattaatgg aagaagttgg tttccctaaa ggaacaatta
    13861 atcttattct aggtgcaggt tctgaagttg gtgacgtaat gtcaggtcat aaagaggttg
    13921 accttgtatc atttacaggt ggcattgaga ctggtaagca tattatgaaa aatgctgcta
    13981 ataatgttac gaatattgcc ttggaacttg gcggtaaaaa tccaaacatt atctttgatg
    14041 atgctgattt tgaattggca gtagaccaag cgttaaatgg tggatatttc catgcaggtc
    14101 aagtttgttc agcaggatca agaatattag tacaaaacag tattaaagac aaatttgagc
    14161 aagcacttat tgatcgcgtg aaaaaaatca aattaggtaa tggttttgat gctgatactg
    14221 aaatgggacc agtgatttca acagaacatc gtaataagat cgaatcttat atggatgtag
    14281 ctaaagcaga aggcgcaaca attgctgttg gtggtaaacg tccagataga gatgatttaa
    14341 aagatggtct attcttcgag ccaacagtca ttacaaattg tgatacgtca atgcgtattg
    14401 tacaagaaga ggttttcgga cctgtcgtta ctgtagaagg ctttgaaact gaacaagaag
    14461 cgattcaatt agcgaatgat tctatatatg gtttagcagg tgctgtattt tctaaagata
    14521 ttggaaaagc acaacgcgtt gctaacaagt tgaaacttgg aacggtgtgg attaatgatt
    14581 tccatccata ttttgcacaa gcgccatggg gtggatacaa acaatcaggt atcggtagag
    14641 aattaggcaa agaaggctta gaagagtacc ttgtttcaaa acacatttta acaaatacaa
    14701 atccacaatt agtgaattgg tttagcaaat aaaaattaga taaggtgagt gccattgtaa
    14761 gaacacaaga cactcacttt gttttgtata agtggcgaaa tgttgattga taatttggac
    14821 taaacgcaaa atgaatcata gattatttca ttactgttag taacaatcgt aaaaggaaaa
    14881 gcgagtgttt tggttagcta agtttagcaa ttcaacgata accaatcagc cactaacaaa
    14941 tatttcatgc aatactcact ttgaaataca acaaactttg gaggtcataa cgatgagtaa
    15001 caaaaacaaa tcatatgatt atgtcatcat tggaggaggc agtgcaggtt ctgtactagg
    15061 taatcgtctg agtgaagata aagataaaga agtcttagta ttagaagcgg gtcgcagtga
    15121 ttatttttgg gatttattta tccaaatgcc tgctgcgtta atgttccctt caggcaataa
    15181 attttacgat tggatttatt caacagatga agaaccacat atgggcggtc gtaaagtagc
    15241 acatgcccga ggtaaagttt taggtggatc aagttcgatt aatggcatga tttatcaacg
    15301 tggtaatcca atggactatg aaggctgggc agaaccagaa ggtatggaaa cttgggattt
    15361 tgcgcactgt ttaccgtatt ttaaaaaatt agaaaaaaca tacggtgcag cgccttatga
    15421 taaatttaga ggccatgatg gaccaattaa gttaaaacga gggccagcaa cgaatccttt
    15481 attccagtca ttctttgatg caggtgttga agcaggctat cataaaacac ctgatgtgaa
    15541 tggatttaga caagaaggtt ttggaccgtt cgatagtcaa gtacatcgtg gtcgccgaat
    15601 gtcagcttca agagcatatt tacatccagc gatgaagcgt aaaaacttaa ccgttgaaac
    15661 acgtgccttt gtaactgaaa ttcattatga aggtagaaga gcaactggtg ttacgtataa
    15721 gaaaaatggc aaactacata ccatcgatgc taatgaagtc attttgtctg gtggggcatt
    15781 caatacgcca caattactac aattatctgg tatcggtgat tcagagttcc taaaatcaaa
    15841 aggcattgag ccacgtgttc atttacctgg tgtgggtgaa aactttgaag atcacttaga
    15901 ggtatacatt caacataaat gtaaggaacc tgtatcatta cagccaagct tagatatcaa
    15961 gcgcatgcca tttattggtt tacaatggat tttcacacgt acaggtgcag cagcatctaa
    16021 ccattttgaa ggtggcggat ttgtacgttc aaataacgaa gttgattatc ctaacttaat
    16081 gttccatttc ttaccaatag ctgtaagata tgatggtcaa aaggcagcgg tcgcacatgg
    16141 ttatcaagtt cacgtaggac caatgtattc aaactctcgt ggtagcttga aaatcaaatc
    16201 taaagatcca ttcgaaaaac caagtatccg ctttaattat ttatcaacag aagaagataa
    16261 aaaagaatgg gtagaagcaa ttcgtgtagc aagaaatatc ttatctcaaa aagcaatgga
    16321 tccattcaat ggtggagaaa tttcaccagg accagaagtg caaacagacg aagaaattct
    16381 tgattgggtt cgtagagatg gtgaaacagc attacaccca tcttgtagtg ctaaaatggg
    16441 tcctgcttca gatccaatgg cagtagttga cccgttaaca atgaaagtcc acggtatgga
    16501 aaatttacgt gtagttgatg catcagctat gccacgtacg acaaatggca atatacacgc
    16561 acctgtttta atgttagctg aaaaagcagc agacattata cgtggcagaa agcctttaga
    16621 gccacaatat attgattatt ataagcatgg tgttcatgat gaaaatgaag gtgcaatcga
    16681 agtaaaacca tacgctaaat aaatttatat agtagactct tagtctggca tctattgtat
    16741 tggtgccagg cttttttgcg ttttatctaa atgagaagtt aatgtgttgt tcaaaaacgg
    16801 tgtgtgagta tatgaactga aaatagagtt aattgtacat tgtgatgatt aattgaccta
    16861 attggcagac attaaaagag tgcacaacct taaaaagttc actaaataga catttaattg
    16921 tgttttcttg aaagctttcg tggtaggatt tatataagta aattgagaga aggaatacta
    16981 tgagctttta cgaatatata caaacattta aagatgataa aacaccatta ggcgaattag
    17041 cgatttggat taaagaagat gattcattcc caaaacaaga gaagctaaca gaaaacattt
    17101 tgtcttattt tcatcaaatg tccaatatag atcatgagtt tttagaaatt gtaaaaagat
    17161 cactttctct gtatgatcaa ttaaaatcgt aaaatcagaa ataaagttag ctaaaataaa
    17221 aggccggtga tttcacagat gaagttgtcg gctattttca tgactaatgt taaataatga
    17281 tcatgaaatt gaagacacaa aaatagtggg cgactcctgt cccccatttg catgaaatat
    17341 taatactttt tacaaagtct tatcttttaa aaagaagcca
//
LOCUS       DAKTLH010000045        17080 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.045, whole genome shotgun sequence.
ACCESSION   DAKTLH010000045 DAKTLH010000000
VERSION     DAKTLH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17080
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.045"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..554
                     /gene="capA"
                     /locus_tag="O0394_001264"
     CDS             <1..554
                     /gene="capA"
                     /locus_tag="O0394_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HCY0499349.1"
                     /translation="LSPKYQANTQILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKS
                     PRILDEVSKDLNDKYSPSKLSSMLTITNQENTQLINIQVKSGHKQDSEKIANSFAKVT
                     SKQIPKIMSVDNVSILSKADGTAVKVAPKTVVNLIGAFFLGLVVALIYIFFKVIFDKR
                     IKDEEDVEKELGLPVLGSIQKFN"
     gene            570..1256
                     /gene="cap8B"
                     /locus_tag="O0394_001265"
     CDS             570..1256
                     /gene="cap8B"
                     /locus_tag="O0394_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HCY0499350.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIGSERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKTKVDKS
                     SSYYHYYGDE"
     gene            1259..2023
                     /gene="cap8C"
                     /locus_tag="O0394_001266"
     CDS             1259..2023
                     /gene="cap8C"
                     /locus_tag="O0394_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HCY0499351.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMMDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVINGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAIRQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            2043..3867
                     /gene="cap8D"
                     /locus_tag="O0394_001267"
                     /pseudo
     CDS             2043..3867
                     /gene="cap8D"
                     /locus_tag="O0394_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
     gene            3857..4885
                     /gene="cap8E"
                     /locus_tag="O0394_001268"
     CDS             3857..4885
                     /gene="cap8E"
                     /locus_tag="O0394_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HCY0499352.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKQFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVYYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            4898..6007
                     /gene="cap8F"
                     /locus_tag="O0394_001269"
     CDS             4898..6007
                     /gene="cap8F"
                     /locus_tag="O0394_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HCY0499353.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            6011..7135
                     /gene="cap8G"
                     /locus_tag="O0394_001270"
     CDS             6011..7135
                     /gene="cap8G"
                     /locus_tag="O0394_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HCY0499354.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGL
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            7138..7764
                     /locus_tag="O0394_001271"
     CDS             7138..7764
                     /locus_tag="O0394_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCY0499355.1"
                     /translation="MRIAIEKIIGLLKNQSSKESNVKIHRLAYITNSKFDGNNYIDRW
                     CKIRNSHIGEYSYIGFGSDFNNVEVGRYCSISSDVKIGLGKHPTHFFSSSPIFYSNNN
                     PFNIKQKFIDFNDQPSRTTIKNDVWIGANVIIMDGLTINTGAVIAAGSVVTKNVGAYE
                     VVGGVPAKVIKKRFDNKTIEKLLESKWWEKTPDKLKGFSVEYLNKKDT"
     gene            7769..8878
                     /locus_tag="O0394_001272"
     CDS             7769..8878
                     /locus_tag="O0394_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0499356.1"
                     /translation="MRILNIVSSNIVQDPRVLKQIETIKGVTDDYKIVGMNNSQATNK
                     RLENLDCNYRLLGSKVDPKNILSKLIKRIRFATGVIREIKAYKPDVIHANDFDVLLMV
                     YLSNYKKANIVYDAHEIYAKNAFINKVPLISKFVESIEKHIVKHRVNAFVTVSHAAKE
                     YYQSKGYKKEANVITNAPILNDSREFKEIENFKEIVYQGQIVMDRGYEEFIIASSAFK
                     QNAPSFIIRGFGPHEEVIKELISYNPENIRLDKPVEVKELVDKLAESNVGVVLTKPVS
                     INFEYTVSNKIFECIHAGLPVILSPVKEHIYLNEKYKFGIVLKEVTPLEIEKAVRKLR
                     DNHDLFNHLRQNAIKASKILNWQIESERLVELYKF"
     gene            8892..10058
                     /locus_tag="O0394_001273"
     CDS             8892..10058
                     /locus_tag="O0394_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCY0499357.1"
                     /translation="MKFFVLCAIISMNIFIVISTFTKEVLGFPIEPVYYSTMVGIALI
                     TTVFAIYKIIVTQEIPRGLILLIAICLLYLAFYYFSPDKEEKLAKNNILFFLTWAVPA
                     AISGIYIKYINKATVERFFKLVFFIFSVSFIFVILIPKLTGEIPSYINFGLMNYQNAS
                     YLSAFTAGLGIYFIMKGSVKHKWIYVLFTIIDIPIVFIPGGRGGAILLILYGLFAFIL
                     ITFKRGIPIAVKSIMYIFALSISSVLIYFLFTKGSNTRTFSYLQGGTLNLEGTSGRGP
                     IYEKGIYFIQQSSLLGYGPFNYYKLIGNIPHNIIIELILSFGLLGFFIIMICILLLVY
                     KMIRNYDPNTIDLLVMFIAIYPITLLMFSSNYLVVSEFWFVLFYFITKGRRHHG"
     gene            10051..11256
                     /locus_tag="O0394_001274"
     CDS             10051..11256
                     /locus_tag="O0394_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCY0499358.1"
                     /translation="MAKKVFIMDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSI
                     LTIYTIITITSVVLGNTLNNIRLINMNLYKSNHYYWKFASILLISILIESIALIIVFL
                     YFFNLNIIDIIFLILLNILMCLRIYLNVFFRMTLKYNQILYIALIQFLGLLIGLFLYY
                     LTQNWIVCFITSELFATIYTLVKLRGLTIGEYQSEDNNVVKDYVMLLSTNSLNNLNLY
                     LDRLILLPIIGGTAVTISFLSTFIGKMLATFLYPINNVVLSYISVNESDNIKKQYLKT
                     NLIAIAALCLVMIICYPITIIIVSLLYNIDSSLYSKFIILGNIGVLFNAVSIMIQTLN
                     TKHASITLQANYMTLHTITFIFITILMTIAFGLNGFFWTTLFSNIIKYVILNIIGLKS
                     KFINKKDVD"
     gene            11257..12462
                     /gene="cap8L"
                     /locus_tag="O0394_001275"
     CDS             11257..12462
                     /gene="cap8L"
                     /locus_tag="O0394_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HCY0499359.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQSQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKALVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            12473..13030
                     /gene="cap8M"
                     /locus_tag="O0394_001276"
     CDS             12473..13030
                     /gene="cap8M"
                     /locus_tag="O0394_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HCY0499360.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            13030..13917
                     /gene="capN"
                     /locus_tag="O0394_001277"
     CDS             13030..13917
                     /gene="capN"
                     /locus_tag="O0394_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HCY0499361.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGHVGKSDQVDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSMLNKYFNKLSVFRKLFGNLIYSNTLYENNNALEIIPG
                     KMSLVIADIMDETTTKDKA"
     gene            13971..15233
                     /gene="cap8O"
                     /locus_tag="O0394_001278"
     CDS             13971..15233
                     /gene="cap8O"
                     /locus_tag="O0394_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HCY0499362.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPDASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILSFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTEACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKVLSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            15310..16455
                     /gene="cap8P"
                     /locus_tag="O0394_001279"
     CDS             15310..16455
                     /gene="cap8P"
                     /locus_tag="O0394_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HCY0499363.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVVLLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRTDKPDEFVPLRHK"
     gene            complement(16520..16846)
                     /gene="isdI"
                     /locus_tag="O0394_001280"
     CDS             complement(16520..16846)
                     /gene="isdI"
                     /locus_tag="O0394_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HCY0499364.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(16853..>17080)
                     /locus_tag="O0394_001281"
     CDS             complement(16853..>17080)
                     /locus_tag="O0394_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCY0499365.1"
                     /translation="KIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAV
                     RVGLIIMVIIQTAVLFTFVKTLPKSNHKIEE"
BASE COUNT         6078 a         2400 c         3209 g         5393 t
ORIGIN      
        1 tcttatcacc taaatatcaa gctaatactc aaattttagt gaatcaaact aagggtgaca
       61 atcctcagtt tatggcgcaa gaggttcaaa gtaatattca acttgtaaat acgtataaag
      121 aaattgttaa aagtcctaga attttagatg aggtgtcaaa ggacttaaat gataagtatt
      181 caccatctaa attgtcgagt atgttgacaa ttacaaacca agaaaatacg caacttatca
      241 acatccaagt taaaagtggt cataaacaag attcggaaaa aattgcgaat agcttcgcta
      301 aagttacaag taaacaaatt ccgaagatta tgagtgtgga taacgtatca attttatcta
      361 aagcagacgg tacagcagtt aaagtcgcac caaaaactgt agtgaatcta atcggtgcat
      421 tctttttagg attagttgtc gcgcttatat atatcttctt caaagtaatt ttcgataagc
      481 gaattaaaga tgaagaagat gtagagaaag aattaggatt gcctgtattg ggttcaattc
      541 aaaaatttaa ttaaggatgg ttgctactta tgtcaaaaaa ggaaaatacg acaacaacac
      601 tatttgtata tgaaaaacca aaatcaacaa ttagtgaaaa gtttcgaggt atacgttcaa
      661 acatcatgtt ttcaaaagca aatggtgaag taaagcgctt attggttact tctgaaaagc
      721 ctggtgcagg taaaagtaca gttgtatcga atgtagcgat tacttatgca caagcaggct
      781 ataagacatt agttattgat ggcgatatgc gtaagccaac acaaaactat atttttaatg
      841 agcaaaataa taatggacta tcaagcttaa tcattggtcg aacgactatg tcagaagcaa
      901 ttacgtcgac agaaattgaa aatttagatt tgctaacagc tggccctgta cctccaaatc
      961 catctgagtt aattgggtct gaaaggttca aagaattagt tgatctgttt aataaacgtt
     1021 acgacattat tattgtcgat acaccgccag ttaatactgt gactgatgca caactatatg
     1081 cgcgtgctat taaagatagt ctgttagtaa ttgatagtga aaaaaatgat aaaaatgaag
     1141 ttaaaaaagc aaaagcactt atggaaaaag caggcagtaa cattctaggt gtcattttga
     1201 acaagacaaa ggtcgataaa tcttctagtt attatcacta ttatggagat gaataagtat
     1261 gattgatatt cataaccata tattgcctaa tatcgatgac ggtccgacaa atgaaacaga
     1321 gatgatggat cttttaaaac aagcgacaac acaaggtgtt acagaaatca ttgtaacatc
     1381 acatcactta catcctcgat ataccacacc tatagaaaaa gtgaaatcat gtttaaacca
     1441 tattgaaagc ttagaggaag tacaagcact aaatctaaag ttttattatg gtcaggaaat
     1501 aagaattacc gatcaaatcc ttaatgatat tgatcgaaaa gttattaacg gtattaatga
     1561 ttcacgctat ttactaatag aatttccatc aaatgaagtt ccacactata ctgatcaatt
     1621 atttttcgaa ttacagagta aaggctttgt accgattatt gcacatccag agcggaataa
     1681 agcaataagg caaaaccttg acatactata cgatttaatt aacaaaggtg ctttaagtca
     1741 agtgacaacg gcgtcattag cgggtatttc cggtaaaaaa attagaaaat tagcaattca
     1801 aatgattgaa aacaatctga cacatttcat cggttcagat gcgcataaca cagaaatcag
     1861 accgttctta atgaaagact tatttaatga taagaaatta cgtgattatt atgaagatat
     1921 gaacggattt attagtaatg cgaagttagt tgttgatgat aaaaaaattc ctaaacgaat
     1981 gccacaacaa gattataaac agaaaagatg gtttgggtta taaacagcaa atgaggggtt
     2041 ttatggcaca tttatctgtg aaattgcggc ttttaatact agcattaatc gattcactga
     2101 tagtgacatt ttcagtattc gtaagttatt acattttaga accgtatttc aaaacatatt
     2161 ctgtcaaatt attaatattg gcagctatat cactattcat atcgcatcat atttcagcat
     2221 ttatttttaa tatgtatcat cgagcgtggg aatatgccag tgtgagtgaa ttgattttaa
     2281 ttgttaaagc tgtgacgaca tctatcgtta ttacgatggt ggtcgtgaca attgttacag
     2341 gcaatagacc gttttttaga ttgtatttaa ttacttggat gatgcacttg attttaatag
     2401 gtggctcaag gttattttgg cgtatttatc ggaaatacct tggaggtaag tcatttaata
     2461 agaagccaac tttagttgtt ggtgctggtc aagcaggttc aatgctgatt agacaaatgt
     2521 tgaaaagtga cgaaatgaaa cttgaaccgg tattagcagt cgatgatgac gaacataaac
     2581 gcaatatcac aattactgag ggtgtaaaag tccaaggtaa aattgcggat attccagaac
     2641 tagtgaggaa atataagatt aaaaaaatca tcattgcaat tccaactatt ggtcaagagc
     2701 gtttgaaaga aattaataat atttgccata tggatggcgt tgagttattg aaaatgccaa
     2761 atatagaaga cgtcatgtct ggtgagttag aagtgaacca acttaaaaaa gttgaagtag
     2821 aagatttact aggcagagat cctgttgaat tagatatgga tatgatatca aatgaattga
     2881 cgaataaaac tattttagtt acgggtgcag gtggttcaat aggatcagaa atttgtagac
     2941 aagtttgtaa tttctatcca gaacgtatta ttctacttgg ccatggtgaa aacagtattt
     3001 atttaatcaa tcgtgaattg cgaaatcgct tcggaaaaaa atgttgatat cgttcctatt
     3061 atagcggatg tgcaaaatag agcgcgtatg tttgaaatta tggaaacgta taaaccatac
     3121 gcagtttatc atgcagcagc acacaagcac gtgccgttaa tggaagacaa ccctgaagaa
     3181 gcagtacgta ataatatttt aggtacgaaa aatactgctg aagctgctaa aaatgcagag
     3241 gtaaagaaat tcgttatgat ttctacggat aaagccgtta atccgcctaa tgtcatggga
     3301 gcttcaaagc gaattgcaga aatgattatt caaagtttaa atgatgaaac gcatcgaaca
     3361 aattttgttg cagtgagatt tggtaatgta cttggatcga gaggatctgt gattccactt
     3421 ttcaaaagtc aaattgaaga aggtgggcca gttactgtga cacatcctga aatgacacgt
     3481 tactttatga caattcctga agcttctaga ctagttttgc aggcaggggc attagcagaa
     3541 ggtggcgaag tatttgtgct agatatggga gaaccagtga aaattgtaga tttggcacgt
     3601 aatttaatta agctaagtgg taaaaaagaa gacgacatac gcattactta tacagggatt
     3661 agacccggcg aaaaaatgtt tgaagagctt atgaataaag atgaggttca tcctgaacaa
     3721 gtatttgaaa aaatttatcg tggcaaagta caacatatga aatgtaatga agttgaagcg
     3781 attattcaag acatcgtcaa tgactttagt aaagaaaaaa ttattaacta tgccaatggc
     3841 aaaaagggag ataattatgt tcgatgacaa aattttatta attactgggg gcacaggatc
     3901 attcggtaat gctgttatga aacagttttt agattctaat attaaagaaa ttcgtatttt
     3961 ttcacgcgat gagaaaaaac aagatgacat tcgaaaaaaa tataataatt caaaattaaa
     4021 gttctacatt ggtgatgtgc gtgatagtca aagtgtagaa acagcaatgc gagatgttta
     4081 ttacgtattc catgcagcag ctttaaaaca agtgccgtca tgtgaattct ttccagttga
     4141 ggcagtgaag acaaatatta ttggtacaga aaatgtctta caaagtgcta ttcatcaaaa
     4201 tgttaaaaaa gtcatatgtt tatctacaga taaggcagcg tatcctatta atgctatggg
     4261 tatttcaaaa gcaatgatgg aaaaagtatt cgtagccaaa tcaagaaata ttcgtagtga
     4321 acaaacgctt atttgtggta caagatacgg taatgtgatg gcttcaagag gatcagtaat
     4381 acctttgttt atcgacaaaa tcaaagctgg agaaccttta acgattacag atcctgatat
     4441 gacaagattt ttaatgagct tagaagatgc ggtagaacta gttgttcatg catttaagca
     4501 tgcagagaca ggagatatta tggttcaaaa agcaccaagc tcaacggtag gggatcttgc
     4561 gaccgcatta ttagaattgt ttgaagctga taatgcaatt gaaatcattg gtacgcgaca
     4621 tggagagaaa aaagcagaaa cattgttgac gagagaagaa tacgcacaat gtgaagatat
     4681 gggtgattat tttagagtgc cggcagactc cagagattta aattatagta attatgttga
     4741 aaccggtaac gaaaagatta cgcaatctta tgaatataac tccgataata cacatatttt
     4801 aacggtggaa gagataaaag aaaaactttt aacactagaa tatgttagaa acgaattgaa
     4861 tgattataaa gcttcaatga gataggagag attgacgttg aatattgtaa ttacaggagc
     4921 aaaaggtttt gtaggaaaaa acttgaaagc agatttaact tcaacgacag atcatcatat
     4981 tttcgaagta catcgacaaa ctaaagagga agaattagag tcagcattgt tgaaagcaga
     5041 ctttgtcgtg catttagcgg gtgttaatcg acctgaacat gacaaagaat tcagcttagg
     5101 aaacgtgagt tatttagatc atgtacttga tatattaact agaaatacga aaaagccagc
     5161 gatattatta tcgtcttcaa tacaagcaac acaagataat ccttatggtg agagtaagtt
     5221 gcaaggggaa cagctattaa gagagtatgc cgaagagtat ggcaatacgg tttatattta
     5281 tcgctggcca aatttattcg gcaagtggtg taagccgaat tataactcag tgatagcaac
     5341 attttgttac aaaattgcac gtaacgaaga gattcaagtt aatgatcgga atgttgaact
     5401 aacgctaaac tacgtggatg atatcgtcgc tgaaataaag cgtgctattg aaggaactcc
     5461 aacgattgaa aatggtgtac ctacagtacc aaacgtattt aaagtgacat tgggagaaat
     5521 tgtagattta ttatacaagt tcaaacagtc acgtctcgat cgaacattgc cgaaattaga
     5581 taacttgttt gaaaaagatt tgtatagtac gtatttaagc tatctaccta gtacagactt
     5641 tagttatccc ttacttatga atgtggatga taggggttct tttacagaat ttataaaaac
     5701 accggatcgt ggtcaagttt ctgtaaatat ttctaaacca ggtattacta aaggtaatca
     5761 ctggcatcat actaaaaacg aaaaatttct agtcgtatca ggtaaagggg taattcgttt
     5821 tagacatgtt aatgatgatg aaatcattga atattatgtt tctggcgaca aattagaagt
     5881 tgtagacata ccagtaggat acacacataa tattgaaaat ttaggcgaca cagatatggt
     5941 aactattatg tgggtgaatg aaatgtttga tccaaatcag ccagatacgt atttcttgga
     6001 ggtatagcgc atggaaaaac tgaaattaat gacaatagtt ggtacaaggc ctgaaatcat
     6061 tcgtttatca tcaacgatta aagcatgtga tcaatatttt aatcagatat tagtacacac
     6121 tggtcaaaat tatgattata cattgaatca aattttcttt gatgatttgg aattaagaca
     6181 accggaccac tacttagagg cagttggaag taaccttgga gaaacgatgg ggaatattat
     6241 tgcgaagaca tatgatgttt tattacgcga acaaccagat gcacttttaa ttcttggtga
     6301 tacaaatagt tgtttagcag cagtatctgc taaacgatta aagattcctg tgttccacat
     6361 ggaagcgggt aatagatgct ttgatcagaa tgtacctgaa gaaatcaatc gtaaaattgt
     6421 tgaccatgtc agtgatgtga atctacctta tacggaacat agcagacgtt atttattaga
     6481 tgaaggcctc aataaagcga atatctttgt gacaggatca ccgatgacag aagtgataga
     6541 agcgcatcga gataaaatta atcacagtga cgttttaaat aaactaggat tagaaccgca
     6601 acaatacatt ttagtatctg cgcatagaga agagaatatc gataatgaaa agaattttaa
     6661 atcattaatg aatgcgataa atgatattgc caaaaagtat aaaatgcctg tgatttattc
     6721 aacgcatcca agaagttgga agaaaattga agaaagtaaa tttgaatttg atccattagt
     6781 taaacagtta aagccatttg gtttctttga ttataatgca ttgcaaaaag atgcatttgt
     6841 tgtgctatca gatagtggaa cattgtcaga agagtcgtct attttgaagt tccctggtgt
     6901 ccttattcga acttccacag aaagaccgga agtactagat aaaggtacgg ttattgtagg
     6961 tggtattacc tataacaatc taatccaatc cgttgaacta gcaagagaga tgcaaaacaa
     7021 taacgaaccg atgattgatg ctattgatta taaagacact aacgtttcga caaaggtagt
     7081 taaaattatt caaagctata aagatattat caatcgaaat acttggagga aatgacgatg
     7141 aggatagcga ttgaaaagat aattggtttg ctgaaaaacc agtcctctaa agaatcgaat
     7201 gttaagattc atcgcttggc gtatattaca aactcaaaat ttgatggcaa taactatata
     7261 gatagatggt gtaaaatcag gaattctcac attggtgaat acagttatat tggatttggt
     7321 agtgatttta ataatgtaga agtaggaaga tattgttcga tatcttcgga tgtaaaaatt
     7381 gggttaggaa aacatcctac acactttttt agctcatcac cgatttttta ttctaataat
     7441 aatccattta acataaagca aaagtttata gactttaatg accaaccaag ccgtacaaca
     7501 attaaaaatg atgtgtggat tggtgcaaat gtaattatta tggatggttt aacaataaat
     7561 actggtgcag tcatagcagc cggctcagtt gttactaaaa atgtaggagc atatgaggtt
     7621 gttggtggtg ttcctgcaaa agtgattaag aagcgatttg acaataaaac aattgaaaaa
     7681 cttttggaaa gcaagtggtg ggagaaaacg cctgacaaac taaaaggatt ttcggttgaa
     7741 tatttaaata aaaaggatac ttaatgatat gagaatttta aatattgtat cgagtaatat
     7801 tgttcaagac ccaagggtac ttaaacaaat agaaacaatt aaaggcgtta cggatgatta
     7861 taaaattgtt ggaatgaata attcacaagc tactaataag cgattggaaa atttagattg
     7921 taattatcgt ttgttaggta gcaaggtaga tccaaaaaat attctttcta aattaattaa
     7981 gcgtataaga tttgcaacag gtgttatccg agaaattaaa gcttataaac ctgacgtgat
     8041 tcatgcaaat gatttcgacg tattattaat ggtctattta agcaattata aaaaagctaa
     8101 tattgtttat gatgcgcatg aaatatatgc gaaaaatgcc tttattaata aagttccact
     8161 tatttcaaag tttgtagaaa gtatagaaaa acacatagta aaacatcgtg ttaatgcctt
     8221 cgtaacagta agtcatgcag caaaagaata ttatcaatct aaaggatata agaaggaagc
     8281 gaatgttatt acgaatgcac ctattttaaa tgatagcaga gaatttaaag aaatcgaaaa
     8341 ctttaaagaa attgtatatc aaggtcaaat tgtaatggac agaggatatg aagagtttat
     8401 tattgcttca tcagctttta aacaaaatgc tccttcattc ataattcgag ggtttggtcc
     8461 gcatgaagaa gtgataaaag aactgattag ttataacccg gaaaatatta ggttggataa
     8521 accagttgaa gtaaaagaat tggttgataa gttagcagaa agtaatgttg gtgttgtctt
     8581 gacgaaacca gtatctatta attttgaata tacagtatct aataaaattt ttgaatgtat
     8641 acatgctggt ttaccagtaa ttttatctcc tgtcaaagag catatttatc tcaatgaaaa
     8701 atataaattt ggcattgttc taaaggaagt tacgccgtta gaaattgaaa aggcggttag
     8761 aaaattaaga gataatcacg atttgtttaa tcatttacgt caaaatgcaa ttaaggcgtc
     8821 taaaattttg aattggcaaa tagagagtga acggttagta gaattatata aattttaaag
     8881 agaggtagac tatgaaattt tttgtacttt gtgcaattat cagcatgaac atatttatag
     8941 taatctctac atttactaaa gaagtattag ggttccctat agagccggtg tattactcaa
     9001 ccatggttgg tatagcatta attactacgg tgtttgctat ttataagata attgtcaccc
     9061 aagaaattcc gcgagggtta atattattaa ttgctatatg tttgctttat ctagcttttt
     9121 attatttttc accagataag gaagagaaac tagctaaaaa taatattcta ttctttttaa
     9181 catgggcagt tccagcggca attagtggta tttatattaa atatataaac aaggctacgg
     9241 tagaaagatt ttttaaatta gtatttttca tattttctgt ttcatttatt tttgtaattt
     9301 taataccaaa acttacaggt gagataccta gctatatcaa ttttggactt atgaactatc
     9361 aaaacgcttc gtacctttca gcatttactg ccggattagg catttatttc attatgaaag
     9421 gttcagttaa acataagtgg atatatgttc tatttacaat aattgatatc cctattgtgt
     9481 ttataccagg agggcgtgga ggtgctattt tattaattct ttacggctta tttgcattta
     9541 tacttattac gtttaaaaga ggaataccta tcgcagtaaa aagcattatg tatatttttg
     9601 cattaagcat atctagtgta ttgatttact ttctttttac aaaaggttcg aatactagaa
     9661 cattttcata tctacaaggt ggaacactta atttagaagg tacttctgga agaggaccga
     9721 tttatgaaaa aggtatttac tttattcaac aaagttcgtt attaggctat gggccattta
     9781 actattataa actaatcgga aatataccac ataacatcat tattgagttg attctatcat
     9841 ttggcttatt agggtttttt atcataatga tttgcatttt gctactagtt tataaaatga
     9901 ttaggaacta tgatccaaac actatagatt tactcgttat gtttatagca atctatccaa
     9961 tcacattatt aatgtttagt tcaaattatt tagttgtaag tgaattttgg tttgtgttgt
    10021 tctattttat tacaaaagga cggcgtcatc atggctaaga aagtttttat tatggatagc
    10081 gtaaagacaa taattggtac gttgcttata gctttaggat tacaattttt agcttatcca
    10141 attattaatc aacgagtagg taatgaagcg ttcggttcta ttttaacgat ttatacaata
    10201 ataacaatca cgagtgttgt attaggcaat acgcttaaca atatacgatt gattaatatg
    10261 aatctataca aatccaatca ttactactgg aaatttgcat cgatactttt aatctcaatt
    10321 ctgattgaga gtatagcttt aattattgta tttctttact tttttaattt gaacatcatc
    10381 gatattatct ttttaattct acttaatatt ttaatgtgtt taaggattta tctgaatgta
    10441 ttttttagga tgactttaaa atataatcag attttgtata ttgctcttat tcaattttta
    10501 ggtttgctga taggactatt tctatattat ttaacccaaa actggattgt ttgttttatt
    10561 accagtgaat tgtttgcaac gatatataca ttggttaaat tacggggatt aactataggc
    10621 gagtatcaaa gtgaagataa taatgtggta aaagattatg tgatgctact gagtacaaat
    10681 agccttaata atttgaatct ctatttagat agattaatct tattaccaat tataggtgga
    10741 acagctgtaa ctatatcatt tctttcaaca tttattggga aaatgttagc tacatttcta
    10801 tatccgatta ataatgtagt actttcatat atttctgtaa atgaaagtga caatataaag
    10861 aagcaatatt tgaaaactaa tctaattgct atagctgccc tatgtttagt catgattata
    10921 tgttatccaa ttacaataat tattgtctct ttactgtata acattgattc aagtttatat
    10981 tcgaagttta ttattttagg taatataggt gttttattca atgcagtgag tattatgatc
    11041 caaactttaa atacaaaaca cgcatcaata acattacaag cgaattatat gacgcttcac
    11101 acgattacat ttatattcat aactatttta atgacaattg cgtttggtct aaatggattc
    11161 ttttggacaa cgctgttcag caacattatt aagtatgtga ttttaaatat tataggttta
    11221 aagtctaaat tcattaataa aaaggacgtc gattagatga gtgaaaaaaa gattttgatt
    11281 ttatgtcagt atttttatcc ggaatatgta tcttctgcga cgttaccaac tcaattggcg
    11341 gaagatttaa ttgcgaatca cattaatgtc gatgtcatgt gtggatggcc atatgaatat
    11401 agtaatcata aacaggtttc taaaaccgag atgcatcgtg gtattcgcat tcgacgtctc
    11461 aagtattcga ggtttaataa caaaagtaag gttggaagga tcatcaattt ctttagttta
    11521 ttttcaaaat tcgtgattaa tatacctaaa atgttgaaat atgatcagat tcttgtttac
    11581 tctaatccac caatcttgcc attaatacca gacgttttac acagactgct taagaaaaaa
    11641 tattcttttg tggtgtatga tatagcacct gataatgcga ttaagacagg tgcaactcgt
    11701 ccaggtagca tgattgataa gctgatgcgt tacattaata gacatgtcta caagaatgct
    11761 gaaaatgtca ttgtccttgg tacggaaatg aaaaactact tactaaatca tcaaatttct
    11821 aaaaatgctg acaatatcca tgtgattcct aactggtatg acatgcgtca attacaagac
    11881 aatcgtatct ataatgacac atttaaagct taccgtgagc aatacgacaa aattttattg
    11941 tatagcggta atatggggca gttacaggat atggagacac ttatctcatt tttaaaatta
    12001 aataaggatc agtctcaaac gttaacaata ctttgtggtc atggtaagaa atttgcagat
    12061 gtcaaaacgg caatagaaga ccatcgtatt gaaaatgtta aaatgtttga gtttttaaca
    12121 ggtacagact atgctgacgt attaaaaatt gcggatgtat gtattgcatc gctgattaaa
    12181 gaaggcgtcg gtttaggcgt gccgagcaag aattatggct atcttgcagc taagaaagcg
    12241 ttggtactca tcatggataa gcaatctgat atcgttcaac atgttgaaca atatgatgcg
    12301 ggtatccaaa ttgataatgg cgatgcacat gccatttata acttcatcaa cactcactcg
    12361 agtaaggaat tgcacgagat gggtgagcgc gcacatcaac tgtttaaaga taaatatacg
    12421 agagaaatta atactatgaa gtattacaat ctgttgaagt gaggagataa ttatgaagcg
    12481 attattcgat gtagtgagtt caatatatgg tttagtagtt ttaagtccga ttctgttaat
    12541 tacagcatta ctaattaaaa tggaatcacc tggaccagcc attttcaaac aaaaaagacc
    12601 gacgattaat aatgaattgt ttaatattta taagtttaga tcaatgaaaa tagacacacc
    12661 taatgttgca actgatttaa tggattcaac atcgtatata acaaagacag ggaaggtcat
    12721 tcgtaagacc tctattgatg aattgccaca attattgaat gttttaaaag gagaaatgtc
    12781 aattgtaggt cctagaccag cgctttataa tcaatacgaa ttaatcgaaa aacgtacaaa
    12841 agcgaacgtg catacgatta gaccaggtgt gacaggacta gctcaagtga tggggagaga
    12901 tgatatcact gatgatcaaa aagtagcgta tgatcattat tacttaacac atcaatctat
    12961 gatgcttgat atgtatatca tatataaaac aattaaaaat atcgttactt cagaaggtgt
    13021 gcatcactaa tgagaaaaaa tattttaatt acaggcgtac atggatatat cggtaatgct
    13081 ttaaaagata agcttattga acaaggacat caagtagatc aaattaatgt taggaatcaa
    13141 ttatggaagt cgacctcgtt caaagattat gatgttttaa ttcatacagc agctttggtt
    13201 cacaacaatt cacctcaagc aaggctatct gattatatgc aagtgaatat gttgctgacg
    13261 aaacaattgg cacaaaaggc taaagctgaa gacgttaaac aatttatttt tatgagtact
    13321 atggcagttt atggaaaaga aggtcatgtt ggtaaatcag atcaagttga tacacaaaca
    13381 ccaatgaacc ctacgaccaa ctatggtatt tccaaaaagt tcgctgaaca agcattacaa
    13441 gaattgatta gtgattcgtt taaagtagca attgtgagac caccaatgat ttatggtgca
    13501 cattgcccag gaaatttcca acggttaatg caattgtcaa agcgattgcc aatcattccc
    13561 aatattaaca atcagcgcag tgcattatat attaaacatc tgacagcatt tattgatcaa
    13621 ttaatatcat tagaagtgac aggtgtgtac catcctcaag atagttttta ctttgataca
    13681 tcgtcagtaa tgtatgaaat acgtcgccaa tcacatcgta aaacggtatt gatcaacatg
    13741 ccttcaatgc taaataagta ttttaataag ttgtcggtct ttagaaaatt attcggcaat
    13801 ttaatataca gcaatacgtt atatgaaaat aataatgcac ttgaaattat tcctggaaaa
    13861 atgtcacttg ttattgcgga catcatggat gaaacgacaa ccaaagataa ggcataagtc
    13921 atctattaaa taaaatcaac atacaaatcg ttttatttgg aggttatagt atgaagttaa
    13981 cagtagttgg cttaggttat attggtttac caacatcaat tatgtttgca aaacatggcg
    14041 tcgatgtgct tggtgttgat attaatcagc aaacgattga taagttacaa agtggtcaaa
    14101 ttagtattga agaacctgga ttacaagagg tttatgaaga ggtactgtca tcgggaaaat
    14161 tgaaggtatc tacaacgcca gatgcatctg atgtttttat cattgccgtt ccgacgccga
    14221 ataatgatga tcagtaccgg tcatgtgaca tttcgctagt tatgcgtgca ttagatagta
    14281 ttttatcatt tttagaaaaa ggaaatacca ttattgtaga gtcgacaatt gcgcctaaaa
    14341 cgatggatga ttttgtaaaa ccagtcattg aaaatttagg gtttacaata ggtgaagata
    14401 tttatttagt gcattgtcca gaacgtgtac tgccaggaaa aattttagaa gaattagttc
    14461 ataacaatcg tatcattggc ggtgtgactg aagcttgtat tgaagcgggt aaacgtgtct
    14521 atcgcacatt cgttcaggga gaaatgattg aaacagatgc acgtactgct gaaatgagta
    14581 agctaatgga aaacacatat agagacgtga acattgcttt agctaatgaa ttaacaaaaa
    14641 tttgcaataa cttaaatatt aatgtattag atgtgattga aatggcaaac aaacatccgc
    14701 gtgttaacat ccatcagcct ggtccaggtg taggcggtca ttgtttagct gttgatccgt
    14761 actttattat tgctaaagac cctgaaaatg caaagttaat tcaaactgga cgtgaaatta
    14821 ataattcaat gccggcctat gttgttgata caacgaagca aatcatcaaa gtgttgagcg
    14881 ggaataaagt cacagtattt ggtttaactt ataaaggtga tgttgatgat ataagagaat
    14941 caccagcatt tgatatttat gagctattaa atcaagaacc agacatagaa gtatgtgctt
    15001 atgatccaca tgttgaatta gattttgtgg aacatgatat gtcacatgct gtcaaagacg
    15061 catcgctagt attgatttta agtgaccact cagaatttaa aaatttatcg gacagtcatt
    15121 ttgataaaat gaagcataaa gtgatttttg atacaaaaaa tgttgtgaaa tcatcatttg
    15181 aagatgtatc gtattataat tatggcaata tatttaattt tatcgacaaa taaaatgtgt
    15241 caaactaggg catacatgat taaggaaaga taagctgtca tgtgtttgaa cttcagagag
    15301 gataatgtta tgaaaaaaat tatggttatt ttcggtacga gacccgaagc aataaaaatg
    15361 gcaccattag taaaagaaat tgatcataat gggaactttg aagcgaacat tgtgattaca
    15421 gcacaacata gagatatgtt agatagtgtg ttaagtatat ttgatattca agctgatcat
    15481 gatttaaata ttatgcaaga tcaacaaaca ttagcaggcc ttacggcgaa tgcacttgct
    15541 aaacttgata gcatcattaa tgaggaacaa ccggatatga ttttagtaca tggtgatact
    15601 acaacgactt ttgtaggaag tttggcagca ttttatcatc aaattccggt cggacatgta
    15661 gaagctggac ttcgaacaca tcagaaatac tcaccatttc ctgaagagtt aaatcgagtc
    15721 atggtaagta atattgctga attgaatttt gcgccaacag taattgcagc taaaaattta
    15781 ctttttgaaa acaaagacaa agagcgtatc tttattactg gaaatacagt tattgacgca
    15841 ttgtcaacaa cagttcaaaa tgattttgtt tcaacgatta ttaataaaca taaaggcaag
    15901 aaagttgttt tactaacagc gcatcgtcgt gaaaatattg gggaaccgat gcatcagatt
    15961 tttaaagcag taagagattt ggcagatgaa tataaagatg ttgtcttcat ttatccaatg
    16021 catcgtaatc caaaggtaag agcgattgcc gaaaaatatt tatctgggag aaatcggatt
    16081 gaattaattg agccattaga tgcgattgag ttccataatt ttacaaatca atcgtacctc
    16141 gtgctgacag attctggtgg tattcaagag gaggctccta catttggaaa acctgtgttg
    16201 gtattaagga atcatacaga gcgtcccgaa ggcgttgagg cgggaacatc gagagtaatt
    16261 ggcacagatt atgacaatat tgttcgaaat gtgaaacaat tgattgagga tgatgaagcg
    16321 tatcaacgta tgagtcaagc gaataatcca tatggtgatg gacaagcatc acgacgtatt
    16381 tgtgaagcaa tagaatatta ttttggattg cgcacagaca agccggatga attcgtacct
    16441 ttacgtcaca aataataaaa aacccctaat catgaagttg gtttagacaa ccagcggtga
    16501 ctaggggttt ttaatatatt tatttttgat agtggtagcc aatatcatat ttgaatactt
    16561 tatttgataa tattggactt tgctgtccat cgtcatcact ttttaaacgt acatttttat
    16621 gagcttcttt aaatacatcg gaattcaacc aattattaaa gctatcttca gattcccaaa
    16681 tagttaagat tttaacttcg tctgtatcct cggtatttaa tgttttagtg acaaacattt
    16741 gttggaagcc ttcaatagtt tcaatacctt gtctattgta aaaacgttca atcgtttctt
    16801 ccgcactgcc tttttgtaat tgtaatctat tttctgccat aaacatgggc aatcactcct
    16861 ctattttatg atttgatttg ggtaatgttt ttacaaatgt aaagagtaca gcggtttgta
    16921 tgataaccat tatgattaat cctacacgga ctgcaagaac atccaccata taaattgaaa
    16981 aacctattac aatgtataag ctaattaaaa ttttaatttt ctgttgtagc gtgtagcctc
    17041 gatgtaaata aaagttttct acatattctt tataaatttt
//
LOCUS       DAKTLH010000046        17025 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.046, whole genome shotgun sequence.
ACCESSION   DAKTLH010000046 DAKTLH010000000
VERSION     DAKTLH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17025
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.046"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            234..1034
                     /locus_tag="O0394_001282"
     CDS             234..1034
                     /locus_tag="O0394_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0499366.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKLRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYNGNELYNYKSFNRQVFQQVVDYLNMKQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGNDSIDIIMPNMTKGQALKRLLDKWEMSPSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLKK"
     gene            complement(1283..2275)
                     /locus_tag="O0394_001283"
     CDS             complement(1283..2275)
                     /locus_tag="O0394_001283"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCY0499367.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
     gene            2545..3216
                     /locus_tag="O0394_001284"
     CDS             2545..3216
                     /locus_tag="O0394_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCY0499368.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQEPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            complement(3336..4142)
                     /locus_tag="O0394_001285"
     CDS             complement(3336..4142)
                     /locus_tag="O0394_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0499369.1"
                     /translation="MAFHDKTATQVTNIVLNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNSQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(4313..4597)
                     /locus_tag="O0394_001286"
     CDS             complement(4313..4597)
                     /locus_tag="O0394_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0499370.1"
                     /translation="MSNLEIKQGENKFYIGDDENNALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLLKAVVEHARENNLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            4824..5258
                     /locus_tag="O0394_001287"
     CDS             4824..5258
                     /locus_tag="O0394_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0499371.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            5336..6304
                     /locus_tag="O0394_001288"
     CDS             5336..6304
                     /locus_tag="O0394_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCY0499372.1"
                     /translation="MINNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALTYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEVRPFNTKRQHG"
     gene            6326..6919
                     /locus_tag="O0394_001289"
     CDS             6326..6919
                     /locus_tag="O0394_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0499373.1"
                     /translation="MEHIFREGQNGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLAFRGQELLTFIKEAAERYDFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFNTRGAQVEEVWVKGHEITETGLTAGQQILGK"
     gene            complement(7024..8103)
                     /locus_tag="O0394_001290"
     CDS             complement(7024..8103)
                     /locus_tag="O0394_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499374.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMVVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(8447..10411)
                     /locus_tag="O0394_001291"
     CDS             complement(8447..10411)
                     /locus_tag="O0394_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HCY0499375.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQNCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKDLQAQIDILKMLMHDRYLD"
     gene            complement(10784..11554)
                     /locus_tag="O0394_001292"
     CDS             complement(10784..11554)
                     /locus_tag="O0394_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0499376.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNEIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            complement(11556..12251)
                     /locus_tag="O0394_001293"
     CDS             complement(11556..12251)
                     /locus_tag="O0394_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0499377.1"
                     /translation="MDVLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNKPQFIILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLE
                     NVLLEIIEREDQA"
     gene            12685..13143
                     /locus_tag="O0394_001294"
     CDS             12685..13143
                     /locus_tag="O0394_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0499378.1"
                     /translation="MKREVILKLQQFIIERENADKRRQYKKENEELDLSLTQFHIIEL
                     IDNNDKVNNKFLSEMLNVSKPAITKSIKKLLAKDLVVESHNEFNKREVNYSLTQKGKK
                     LSYIHDELHEKSVKKYEEVLKVFDDDEMAVIIEFLNRSIEELKKEDGLND"
     gene            13136..14299
                     /locus_tag="O0394_001295"
     CDS             13136..14299
                     /locus_tag="O0394_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0499379.1"
                     /translation="MINSNIRLYILGFLAFFASLIQNIYTPIIPRLYDDFQVPLFWIN
                     ATVGGFIFIVAVMQIVLGRSIDSRDSKKVLLTGLGIVIISSFICAVTHNFILFAISRL
                     FQAIGCGIIPLVTLTLLAKLSTDNGRAQAMANYQIFLSCAPALAPILGSTLGARWDYI
                     GIFSFLLVISIVLFLIIFFIDIPNVEKGIVKLTEKIEEKYLTDKVFITLVTLGFLIFF
                     TYFSILVYLPTLLNNTYDIGVGISGVLFLPITVSVILGSLFYKRFSKKYNEIMILRVT
                     ITGFAIFTLLFGWLNESNVIILSVIIFILGTCVGIVPALLSTIISKRFEHIKGKVLGV
                     FNFVRYIGMTVGALLIGIISQPLVAFYFTTITIMLIVIFLYIKIVDFQLKYAK"
     gene            14289..14387
                     /locus_tag="O0394_001296"
     CDS             14289..14387
                     /locus_tag="O0394_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499380.1"
                     /translation="MPNKLKKAVNHFNRWNDSLLFVYRNLKFMPLC"
     gene            complement(14396..15007)
                     /locus_tag="O0394_001297"
     CDS             complement(14396..15007)
                     /locus_tag="O0394_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCY0499381.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
     gene            15554..16831
                     /locus_tag="O0394_001298"
     CDS             15554..16831
                     /locus_tag="O0394_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0499382.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
BASE COUNT         5859 a         2521 c         3023 g         5622 t
ORIGIN      
        1 taaattagta tatcacacta tattttgtct aaatgataaa tatagtgtga tatttttaca
       61 atattctgaa atttatgttc gcctctgaat gctatatcca gtgtaatgtg ttttgcatat
      121 atgaaagcaa tttcaaaatg tgaatatagg ttcattgtgg tatgacaaac ttcattgctt
      181 gtcatgagat ggatataatg aagacgacta aaagagcaat gaggaggata atcatggtta
      241 aggctattgc ggtagatatg gacgggacgt ttttagattc aaaaaagaca tatgataaac
      301 ttagatttga agcgattttt actgaactta gaaatagaga tattacattt attgctgcga
      361 gtggcaatca atatgcgaag ttgaagtcta ttttcgggga tagagatatg tattttattt
      421 ctgaaaatgg tgcagttatt tataatggca atgagttata taattataaa agctttaatc
      481 gtcaggtgtt tcaacaggtt gtcgattact taaatatgaa gcaaagtatt gatcaactcg
      541 tcatctgtgg tttgaaaagc gcgtatattt taaaacatac ttctgaagcg tttaaagaag
      601 atacgagatt ttattatcat cagttaaaag aaattgacag tctacagcaa ttacctgagg
      661 atgattatgt caaaatagca tttaatatta atcgtgagac gcatccgaat gttgacgaag
      721 aagtagcaac gcaattcagc aatgatatta aacttgtctc aagtgggaac gatagcattg
      781 atattattat gccaaacatg actaaagggc aggcattaaa acgattgtta gataaatggg
      841 aaatgtctcc ttcagagctc atggccttcg gagatgcgaa taatgataaa gatatgttag
      901 cgttcgctaa gcatagttat gtgatggaaa atagtcatga tgaagaattg ttcaatattg
      961 cctctgctgt cgctccaagt aatgataagc agggtgtatt aacgattatc gaacaagaag
     1021 tgttgaaaaa gtaggtagta tatcatcaat gaagcagcta tcatgatact aaatacatgt
     1081 taaatgaaaa ctttgaatac gaatcatcaa atattaatat atctcaaaca ggttaggcaa
     1141 taatgccata gtaaaataag cgcactaaaa tcatttgaat agatgataaa ataaagcgga
     1201 ttttaatcaa atcgattttg atacagatat acaaaataaa aagcaaaggc acatggcgcg
     1261 cctttgcttt taaatatata tattaattta aacgtgtttc acatgtacca gtgttaatga
     1321 cagataatgc tgcgtttaaa ccaccttcaa caaggttttg tactgcttca tcagagaaga
     1381 aagcaatatg tggtgttact aaaattcttt catgttcgat taactctaat aatgttttat
     1441 cgtcaatgtc tttattagtc cagtcatttg tgaagtatgc tgcttcattt tcataagtat
     1501 caatcgcagc acctaacaaa gtaccatcgt tcactgcagc gattaaatca ggtgtattga
     1561 tgactgcacc acgtgctgcg ttaactaaga ttgcaccttt tttcacatga tcaaacattg
     1621 ctttatcgaa taaatgatag ctttctttgt tcgctggaac atgtaaagaa ataatatcgg
     1681 catctttaat agcttcttta acactatctt tataagttaa aaagtctaaa tctttattag
     1741 gataggcgtc ataagctgta attgtagcac caaatcctgc atatatttta gctgtagcag
     1801 caccgatacg acccgtacca ataattgcaa cagtcatatt tttaactggt ttagacatga
     1861 tttctgcttg ccaagtaaaa tcatgtgctt gtacacggcg ttcaatatct gggaagcgac
     1921 gcactaattg tagggcgata gaaacagaat actctgcaat tgtttcaggt gaataactag
     1981 gaacgttaga tatcacaata ttgtgttttt tagctaaatc taaatcatac atatcaaatc
     2041 cagcagtacg ttgtgcaatt tgtttaatac cgtaagattc taatttagga taaacgtcat
     2101 tttctaactt accaaattgc attgtagtta cgccatcgta atcttttaat tgatcgactg
     2161 tagcacttga taatagctct ttagaagtag ttacttcgac attattcttt ttcccccaat
     2221 ttaatgccat ctctttctca taatcacgcg taccaaagaa cataattttt gtcattatta
     2281 aaaacctcgc ttttaaaaga ttgaaaagta aatgagtgaa attaaagatt atgcacatta
     2341 aaatcacgcc acaatttaat tgtgaaaaat atcacaaata tattataaca ctaaatttcc
     2401 caaaattcaa aagtgtgttt tattgcagaa aacttataac atgtgcacaa gttatagtga
     2461 attgcaaacg gattacttta gtctttttaa aacatgaagt ataatttgta tagcaataaa
     2521 tataaaaatg ggaggctatg ttcaatgagc aatatgaatc aaacaattat ggatgcattt
     2581 catttcagac atgcgactaa gcaattcgat ccacaaaaga aagtttcgaa agaagatttt
     2641 gaaacaatat tagagtcagg tagattgtct ccaagttctc ttgggttaga accttggaag
     2701 tttgtcgtga ttcaagatca agcgttacgt gatgaattaa aagcgcacag ttggggcgca
     2761 gcaaaacaat tagatacagc gagccatttt gtgctaattt ttgcgcgtaa aaatgtaacg
     2821 tcaagatcac cgtatgtaca acatatgtta agagatatta aaaaatatga ggcacaaacg
     2881 attccagctg ttgaacaaaa attcgatgca ttccaagcag atttccatat ttctgataat
     2941 gatcaagcct tgtatgactg gtcaagtaaa caaacgtata ttgcattagg caatatgatg
     3001 acgacagccg cattgttagg tattgattca tgtccgatgg aaggttttag tctggataca
     3061 gtgacagaca ttttagcaaa taaagggatc ttagatactg agcaatttgg tttatcagtg
     3121 atggtcgcat ttggctacag acaacaagag ccaccgaaaa ataaaacacg ccaagcttat
     3181 gaagatgtta ttgaatgggt tggaccaaaa gaataaatag aataccgtat gtctaaatat
     3241 ataaaattaa aaagttagca ataaaaaagc ctgcgattac ataaatgaat cgcaggcttt
     3301 tgcgtgaaaa aattgtatta ataaagtatg gatgattatt tttctggaac aaggtcagta
     3361 tttgaatgaa ctgtgatgtc aaacccttct ggtgccgtaa atgtatgtgt tgaggcgtcg
     3421 ggttgataaa tatcaacatg tgttaatcca taactttgtg aattgttttg tcttgcttga
     3481 ttggattgcc aagtattagc agcaatatga tggtgataat gattcgttga cataaatagc
     3541 gcacgtggaa aatcagacac atgttggaat cctaattgtt caatgtaaca ttgatatgct
     3601 gcgtctaaat catgtgtttt taaatgtaag tgtccaatca tgccttttgc tggcattcct
     3661 tgccaacctt catcagtacg atgtgttaat aaggtttggc tatcaacttc taaagtatcc
     3721 attttaactt tgccattttg ccattcccat gaagatgaag gtctatcgcg atagacttca
     3781 ataccattac cttcggggtc gttgaaatat aaagcttcac ttactaaatg atcaccagcg
     3841 ccgatgccca tatttttttg tgccacgaaa tataagaagt tagctagatc ctcagtagtt
     3901 ggtaataaaa atgctatatg aaaaagccct gcttcacgtg gggaagtctg acggccgtct
     3961 tctaataaat gtaacgttag agtatggcca ccagtcccaa cagataatac ggttgtatta
     4021 tcgtcagaac ttttaacgga tagtcctaaa atgtttttgt aaaatgttgt cattaagtct
     4081 aagtctctta cgttcagtac aatgtttgtc acttgtgttg ctgttttatc gtgaaatgcc
     4141 attatgcatc gcctcttttt ctatttttct ataagttagt ataaaaagta taccagaaaa
     4201 gaaaatgaat tgatagcata aagtttgaaa tgcaaaataa ctagtcgttt tgcaatttta
     4261 tattgatgcg aacaaaaaag cgatggtaca gttgcaccat cgcaaaattt atttaaccaa
     4321 gatatacatc ttgatatgaa tcttcttttt ctaacatatg tttggcaaat gaacatgagg
     4381 caataatttt caaattattt tctcgagcgt gttcaacaac tgctttaagt agttttttgc
     4441 caacaccttg accaccaagt tcatcagata cgcctgtatg atcaatgtta atttcattat
     4501 tatccacaaa acggtatgtg atttcagcta aagcattatt ttcatcatca ccaatataga
     4561 atttgttctc gccttgtttg atttcaaggt tactcataca tatcaactcc tatcatgatt
     4621 gattatagta tttccctatt ctattttaac ttaaacgaag tcaaaggtgc atgacagtca
     4681 tgtgacgaca ttgccacatc tatgtagtcg tttttattaa gcacagtttg aaatgaagat
     4741 gaaaacacgt atcttgacat taaatctatt cagctatata atttatctcg aaatcgaaat
     4801 aaaataaaaa agttggtgat catatggatc gaacgaaaca atctctcaat gtttttgtcg
     4861 gaatgaatag ggcgttagac acattagagc aaattacaaa agaagacgta aagcgatatg
     4921 gcttaaatat tactgaattt gcagtgctcg agttgcttta taataaaggt ccgcaaccaa
     4981 ttcaacgtat tagagaccgc gtattaattg caagtagcag catttcatat gttgtaagtc
     5041 aattagagga caaaggttgg attacacgtg aaaaggataa agatgataaa cgtgtatata
     5101 tggcttgttt aactgaaaaa ggtcaaagtc aaatggcaga tattttccct aagcatgctg
     5161 agacattaac aaaagcgttt gatgtgttaa caaaggatga attaacaatc ttacaacaag
     5221 cgtttaagaa actaagtgca caatctacag aagtgtaagg cgtgcactaa aaatttacat
     5281 taaagtatct cgatttcgag ataaatgcac taaaaatata aagagggtat ataaaatgat
     5341 aaataatcat gaattactag gtattcacca tgttactgca atgacagatg atgcagaacg
     5401 taattataaa ttttttacag aagtactagg catgcgttta gttaaaaaga cagtcaatca
     5461 agatgatatt tatacgtatc atactttttt tgcagatgat gtaggttcgg caggtacaga
     5521 catgacgttc tttgattttc caaatattac aaaagggcag gcaggaacaa attccattac
     5581 aagaccgtct tttagagtgc ctaacgatga cgcattaaca tattatgaac agcgctttga
     5641 tgagtttggt gttaaacacg aaggtattca agaattattt ggtaaaaaag tgttgccatt
     5701 tgaagaagtc gatggccaag tgtatcaatt aatttcagat gagttaaatg aaggggtagc
     5761 acctggtgta ccttggaaga atggaccggt tccagtagat aaagcgattt atggattagg
     5821 ccccattgaa attaaagtaa gttattttga cgactttaaa aatattttag agactgttta
     5881 cggtatgaca actattgcgc atgaagataa tgtcgcatta cttgaagttg gcgaaggagg
     5941 caatggtggc caggtaatct taataaaaga tgataaaggg ccagcagcac gtcaaggtta
     6001 tggtgaggta catcatgtgt catttcgtgt gaaagatcat gatgcaatag aagcgtgggc
     6061 aacgaaatat aaagaggtag gtattaataa ctcaggcatc gttaatcgtt tctattttga
     6121 agcattatat gcacgtgtgg ggcatatttt aatagaaatt tcaacagatg gaccaggatt
     6181 tatggaagat gaaccttatg aaacattagg cgaagggtta tccttaccac catttttaga
     6241 aaataaaaga gaatatattg aatcggaagt tagacctttt aatacgaagc gtcaacatgg
     6301 ttaattggaa tgaggaggat ttgtgatgga acatattttt agagaaggac aaaatggtgc
     6361 gccaacacta atattattgc atggtacagg tggtgatgag ttcgatttat taccgttagg
     6421 cgaagcattg aatgaaaatt atcacttgtt aagtattaga ggacaagttt cagaaaatgg
     6481 gatgaaccgt tatttcaaac gtcttggtga aggtgtttat gatgaagaag atttggcatt
     6541 tcgtggacaa gaattgttga cgttcattaa agaagctgct gaacgttatg attttgatat
     6601 tgaaaaagca gtacttgttg gattttcaaa tggatcaaat atagcgatta acttaatgtt
     6661 gcgttcagaa gcaccattta aaaaagcatt gttatatgca ccgttatacc cagttgaagt
     6721 aacgtcaaca aaggatttat cagatgtcag tgtgttgctt tctatgggga aacatgatcc
     6781 aattgtgcca ttagctgcaa gtgaacaagt cattaacttg tttaatacac gtggggcaca
     6841 agtcgaagaa gtttgggtga agggccatga aattacagaa actggattaa cggctggtca
     6901 acaaatactt gggaaataac agttctatta agaagcggac agatggaaaa gatttttact
     6961 tttcatctgc ccgctttttt gattttgaag tgctgtacta aattttacaa tagtatagat
     7021 attttaatcg atatgagatt tgccggtaat acgcttaatt aaacctttat agagtacagg
     7081 tatgagtaag atgaaaccga acaatcccat aatagggaat acttttccaa ttaatgaaat
     7141 gaaaccgata aatgtactaa tataagtgat gacagccatt gtaataataa tgatgaagta
     7201 acgtctgctg aatggaacgc tgaaacgtga cgcaaatgca tacattaatc caacaacagt
     7261 attgtagatg acaagtatca taatgacaga cataataata ccaattgacg gagacatttg
     7321 tgtcgctaat tttaatgtag gtagatctac gtgtttaatt ttatcgaatt gagaaattaa
     7381 acctagatta atcatcatga gtaaaaatgt aatgattaaa ccgccaatca agcccccgta
     7441 taacgttgag tcacgatatt taactttact acccatcact gataagaagc tgaaggcagc
     7501 agcaatttgc aagcttgcat agttaatcgc atcaaaccac catccaggtg ataatgattt
     7561 ctgcttatga atctgagcat cattattagc ggcagtaaaa tcaagatgac ttgttgtgaa
     7621 atagtagacc gcaatcataa tgacaatcgc aattaaaaat ggggtaacac cgccaagcac
     7681 agcaattaaa cgatcgaatt ttagaaacag tgttgctaaa ataaaggcga ctaatatgag
     7741 tgcgctcagc caatacggta agttgaaact ttgatgaatg gttgacgcac cacctgcagt
     7801 cataataata gctaaagaca acataaacat tgttaaaata atatcaaaac ctcttgcaat
     7861 agaggggtat aagaaatagt taattgaatc agaatgattt ctggacttta gatgatgacc
     7921 tgtatgcatg acaaccattc cacctaaagt aatcaatagt cctgttacaa taatgcctga
     7981 aatgctatat gcgccatgac ttgtgaaaaa ctggaaaatt tcttgaccag tagcaaagcc
     8041 ggcaccaacg acaacaccaa caaaggcaaa tgccacaata atggactctt ttaagatacg
     8101 catgatttaa aaatgtccct tcgtaatttt aagtaatata gaaaatgtaa catacatgtt
     8161 aatgaaaaat atagtactaa tatagtattt tgttaaattg gagtagaagc gagggtgtcg
     8221 gtcatttcat taatttatta gttgattttg catttttttg ctgtaaagtt gttataatac
     8281 agttaacagg aattagcata gatacaccaa tcccctcact actcgcaata gtgaggggat
     8341 ttttttcggt gtagctaggt cgcctattta tcatcgtgtt tgcgtagcca atgcgtaaac
     8401 acagtaccac taaataagtg cacgatacat gcatcaaatg tcgtctttag tctaagtaac
     8461 gatcatgcat taacattttc aaaatatcta tttgagcttg aagatcttta ccaatattgg
     8521 tatcacgaat cttcttacgt tgtaattctt tatctacgac gcgctttata gaaagttcat
     8581 cgataccttc ggaaagtatt ttttctttag cgttaaattg ttggtgtgca acgagttgca
     8641 taccgaatga attatacaat agtgtatagc ctgcaatgcc agttgttgac tgataagctt
     8701 ttgaaaagcc accatcaatg acaagcatct ttccatcagc cttgatagga tcttcgccat
     8761 tgatttcttt cactggtgtg tgaccattaa taatgcgtcc ttcatctgga tttaatccga
     8821 aatcactgag cattttacga accatattca catcttcacg aagatgatag tacggattct
     8881 tttcttcttt atgagaagct ttatctgcaa taaagtatcg ctcaaacgta gtcatggcac
     8941 gtttaccaaa tagtgacgaa tatttcccag tccataaata ccaaactaaa tccgtcgata
     9001 agtcatcagt attttctttt tcatcaaatg atttacggac atgatactca aacacatcta
     9061 ataattcttg gccgctgtaa gtatgaccat caatttcaaa tgattccatc tcaccatttt
     9121 catcaactgg aatacaacca tgaatgagta aattgccatt atatggtaag taaagagagc
     9181 ctttacgcat caagaaagac atatgacgac gtaatttttc agattgttgg aatgacaata
     9241 atagtttatt catgacttct tcttcttcag gtagtaattc tgctggatta tcacgattga
     9301 cagtttggaa acatgtgtct ttcaatgggt atgtattacc ataaactgta atttcatttg
     9361 tatcataatt aaccttttca agcacaagac gttcttccat ttcgaaattt ggacgacgtt
     9421 taataattgg tatttctaac ttgaattgaa tcatcgcaat agcttgatga attttagtaa
     9481 tttgactttc ttcacgttga gttaaacgtt cgtgtttgtc aggtcttttt ttaggcttaa
     9541 aagcaggatt atctgcgtca tagtattttt cagctaaagt aagcagtggt cttaaattaa
     9601 tgccataagc gtcttcgata atatctaaat tatcataacg tgcacaaatt cgaagtaagt
     9661 ttgctaagca tacttttgac ccagcatagg ctccaaccca aagcacatca tgattacccc
     9721 attgaatatc tagggaatga taattaatca gtgtatccat aattttatct ggttgtggtc
     9781 cacgatcata aatatcaccg acaacgtgta aatgatcgac gactaagcgt tgtacggaat
     9841 aagcgagtcc aataattaaa tcatctgcct gtttaagttc aattacttgg ttaacaagtg
     9901 tttcgtaata tgatttttta ttttgatatt cattactttt atacagtagt tcctcaataa
     9961 tataaacgta ttgttttggc aatgctttac gcagttttga acgcgtatat tttgaagaac
    10021 aatatttaat taactcaatt aaatgttcga ttgttgtgat ataccagaca ttaagttgac
    10081 cgcaattttg gaaatcactt ttaatcaatt ttaatttgtc ttctggatag tagacaagag
    10141 cagttaagtc attaagctcc ttagttgaaa gtctctcttt gaaaatatca ttgattttcg
    10201 ctcgcacgtt cccagaaccg ttgcgtaata cgtgttggaa agcttcatat tcaccatgta
    10261 aatcgctgac gaaatgttcc gtacctttag gtaattctaa aattgattct aaattgataa
    10321 tttcagttgc aagtttttct ggagtatcaa aattttggga tagtaaatct aaatacttct
    10381 tttttaattc tttttcagta atttgagtca ttacgtgtca ctcctgtagt atatttcaaa
    10441 cgttagttta taaaataaat gcgcttacaa tgattatcaa gaaaacgtta acatttttca
    10501 ataagtatgc ttgaattgat aaaaatagag ggcaggattt tggtattagg aaccgttgca
    10561 gtttagttaa gtaaaagtgg ggaagtgata tatctatttt aaaaatgaga ggagggattt
    10621 gttatattca ctagtttcct aaaaaattac gatatgtaaa atgtggaatt tccaaaattt
    10681 ggatgaaaaa atccccgcac cattatttgg gtgaagggat ttgaaaatga ttttgaatta
    10741 cgatggaata tgtgtgtatt tagacattta atactttaag ttactaaata tttttcttct
    10801 tgaaaataaa tactacaagt attaagaaaa tagaaatata agcaatatta ccaataaaca
    10861 attcatgaag ttctaatttt gtcgacttac taaagccttt ttcaactgtt tgaagcataa
    10921 tattcatcat attaatagga ttccacttta gccagtctat tttttctaaa agtgccgttt
    10981 gaataactgc taaaatagaa cttgcaaaat aaaaaacaat acctacagca atggctactc
    11041 ccgttgaatt tgttgcagat gataatagca acgttaagct taaaactaac caaacgccaa
    11101 caaatgtgcc taaactaact aataataatt gattcaataa agataattga ttaccgctac
    11161 tttcaaatat atttaaatca ttaaagaata aagacccaat aacaattgaa gcaataattg
    11221 taataacaaa ataaattaaa gaaataataa ataatgtgat gattttgcta acaatcatag
    11281 ttgttcttga atattcacga tagagtaaat ttttaatcgt accgtaatga aattccattg
    11341 aaatgattgt acttgcttga ataattaata aaaatgcaaa ccatgaaaaa ccattataag
    11401 cagatgtgaa agattcctgt ggcgtaaaaa tttcattgta ttttgtagca atataacctt
    11461 gagcaaccat tagtagaata aagacaatag gtgcgataaa agttgatttc tttttaaata
    11521 atttgaaaca ttcttgttta attaaagttc ccattttatg cttggtcctc cctttctatg
    11581 atttctagta atacattttc taaatcatta tctttaaatt gtttaagttc ttcttccgat
    11641 gtttctgtaa caattttacc tttgttaata ataaggatag agtttgtgat tttaactaat
    11701 tcacttaaaa tatgactcga aattaagaaa gtaacacctt cttgcgcttt ttggacaatc
    11761 aattcacgta catctcgcac agcttttgga tctaagccat tcattggttc atctaagata
    11821 atgaattgag gtttatttaa aaatgctata gcaattccta atttttgttt cataccaaga
    11881 gaatacgttt tagctttttt atgaatgtat tcatccatat gaagttgtga gacaatttta
    11941 tcgatatctt gagtgttttt tgattcattc aataacttca agttttcata tccagacata
    12001 aaaggatata ttcctggatt ttcaatcaat gcgccgatat tgcttttgct gtccttgctg
    12061 ttaataacat taaaattccc gctttggaaa ctagagtaac ctaatataac tttcattaaa
    12121 gttgttttac ctgcaccatt cgctccaacg agaccaacta tttgtccgcg tttcagctta
    12181 aatgatacat cttcgagaat cgttttgttg cctatcttct ttgttaaatg ttctattgtt
    12241 aaaacatcca tatcactatc tcctttgcta ttaaataatc tgaatcttaa gattttatat
    12301 ataaaattaa ttttatcaca tagcaagtgt tataaaaata tggatttaaa aaactttaat
    12361 aatataaaac ataaatatga atacgagtat ttttgcagca tgcaaatggc attgctacat
    12421 ttattaaaat gcgcaatttg ttatgagtaa cttttttgga tagtaggcac attaaagtgt
    12481 cgaaaacaat ttgtgtggaa gacagggtaa attctgctta tttaaaatga tagtttgtat
    12541 tataggtgga tttgtgaaat gcggaataaa tggtgcataa ctaagtaaat atatgttaat
    12601 tacaacaata tttgacaaaa atgggagtga caacaataat atagttaacc ggttaactaa
    12661 tgaagaggag gaataatagt ggatatgaag agagaagtta ttttaaaatt acaacaattt
    12721 attatagaaa gagaaaatgc cgataaaaga agacagtata aaaaggaaaa tgaggaacta
    12781 gatttatctc taacacaatt tcatatcata gagttgattg ataataatga taaagtgaat
    12841 aataaatttt tgtctgaaat gttaaatgta tcaaaaccag caattactaa atcgataaaa
    12901 aaacttttag cgaaagactt agtagttgaa tcgcacaatg aatttaacaa aagagaagtt
    12961 aattattcat taacacaaaa aggaaaaaaa ttatcttata tacatgatga attacatgaa
    13021 aaatcggtaa aaaaatatga agaggtactt aaagtctttg atgatgatga aatggcagtt
    13081 attatagagt ttttaaaccg tagtatagaa gaattaaaaa aagaagatgg tttaaatgat
    13141 taatagtaat atcagattat atattctagg atttttagca tttttcgcat cattgattca
    13201 aaacatatac acgcctatta ttccgagact atatgacgat tttcaagtac cactattttg
    13261 gataaatgcg acagttggcg gttttatatt tatcgttgca gttatgcaaa ttgtacttgg
    13321 tagaagtata gattctcgtg attcgaaaaa agtactttta acgggattgg gaatagtaat
    13381 catatctagc tttatttgtg cagtgacaca taactttata ttatttgcta tatcaaggtt
    13441 atttcaagca attggttgcg gaattattcc tctcgtgact ttaactttat tagccaaact
    13501 aagtacagat aatggtagag ctcaagctat ggcgaactat caaatatttt tatcatgtgc
    13561 accagctttg gcaccaatct tagggagtac tttaggtgca agatgggatt atattggtat
    13621 ttttagtttt ttgctcgtta tatctatcgt attatttttg attatctttt ttattgatat
    13681 tccaaatgtt gaaaaaggga ttgtaaaatt aactgaaaaa attgaagaaa aatatttgac
    13741 cgataaagtg tttattactt tagtgacatt gggattttta atatttttta cgtacttttc
    13801 aatattagtt tatttaccaa cattattaaa taatacctat gatataggcg ttggaatatc
    13861 aggagttttg tttttaccta ttactgttag tgttatatta ggaagtttat tttataagcg
    13921 attttcaaaa aaatataatg aaataatgat acttcgtgtt actataactg gatttgctat
    13981 atttacgtta ttgtttggat ggttaaacga atcaaatgta attatattat cagtaattat
    14041 atttatatta ggtacttgtg ttggtattgt gccagctttg ctgtctacta taatttctaa
    14101 aaggtttgaa catataaaag ggaaagtgct aggtgtattt aattttgtga gatatattgg
    14161 aatgactgtc ggtgcattat taattggtat catttctcag ccgttggtag ccttttactt
    14221 cacaactata actatcatgt taatagtaat atttctttat ataaagatag ttgactttca
    14281 gctaaagtat gccaaataaa cttaaaaaag cagtgaatca tttcaataga tggaatgatt
    14341 cactgctttt tgtttataga aatttaaaat tcatgccttt atgctaacaa caatattatt
    14401 ttactctctt acgtttgcca atgtaacgta tagcaaagta aatgactgcg ataatgatga
    14461 caacatacat aatacgtgaa tatgtatgaa gaccagtcat caacatacca aagctatcgc
    14521 tcaaagtgcg tcctagtaaa attaaaccga aattccagat tgtagtacct attaatgaaa
    14581 taacggtaaa tgtcacaaca ttcatgcggt ttacaccagc tggaatggta atcaatacac
    14641 gtaatacagg tatgaaacga cagataaata cagcccatac gccatacttt ttaaaccaat
    14701 catttgctcg ctttaaatcc ttacttttca atttaatcca cttaccgtgt cgatcaataa
    14761 aacgatatag acgttcttct gaaatcaaac ggcagatata atataaaatt aacagcccta
    14821 taaacgatgc gatagttgca ataataaata aagttaaaat tgataaatgt gatttaacag
    14881 acataagacc agcaaatgtc agaataattt ccgatggtac gataggtagt acattttcta
    14941 ataaaattaa tataaaaatg gctgcatacc caaaacggct aataaattca gtgataattt
    15001 gttccattat taaaaataag ctcctttaat tttcagcaat tgcagacaaa tagaaactgg
    15061 ataaattcaa atgaataatt aatgatgtcg catgcgatgt tgaaacaaac atttgtctta
    15121 aaatgtatta taagcaataa atcaaaaaat tgcttgctat aagagcaaaa agtcaataaa
    15181 tgatcatcta aacaacaggc gctgcacaat gaatggttaa tgtagttata tgcaatttaa
    15241 aatgaatgtc gaatatgtaa gaaagcctgc aatatacgtt ttaaaacact aggattgcag
    15301 tatttttaat taacaaacga taggctacta tgtaactaaa agtaccttta tatcgtttta
    15361 atagcaaaag ttaagcggaa tttaataaaa tttttataga ttctattgta aacgctttcc
    15421 atgtctgata atattataaa aaataacttt ggtacaattt tgtgacaaag taggggtgga
    15481 catgccagat gtccgaaaat tttaacgttt cagggtaagt tttaaaaaat taattaggga
    15541 agtaggaata gttatgaagg aaaaacgaac taacgtaagg tggatgttcg cgcttgcgtt
    15601 cttctttatt ggggttattg cgtatatgga tagagcaaac atttcatata tcgctaagca
    15661 aatgatggat gatttaggga tgacaaaacc acaatttggt ttattggcat cattcttctc
    15721 tctaggttat gcattaatgc aagtaccatc ggggatgttg gctgaaaaat tcggtccacg
    15781 taagatgatt acaattgcat tagtttggtg gagtgcattt acaatcttaa cgggtatgat
    15841 taagaaccac ggtttaattt atttagtgag attcttattt ggtgtaggtg aggcgccaat
    15901 gtacccttct aatgctgtgt ttaattcatt ttggttctct aaaaatgaaa aaggtagagc
    15961 atcaagtgca ttattagcag gatcatattt cggacctgta ttagcaccaa tagttacaat
    16021 tgctattgtt aacgcattta actggcaagc agtattttac atttttggtg cagtaggtat
    16081 tttaatggct gtattatggg cgattattgc caaagactta cctgagcaac atagaatggt
    16141 taatgaagcg gagaaacgtt tcattatgga aaatcgtgat atcgtagcta ctgaaaagtc
    16201 atcaccacca tggaatgatt tctttaaacg ttttagcttc tatgcaattg caattcaata
    16261 ctttgttgta caatttatca ttacattatt cttaatttgg ttaccgacgt atttaacaga
    16321 agtattccac gttaacttta aagaaatgag cattagttca ttaccttggt tattaatgtt
    16381 cttcttaatc ttatcagcag gtgcaatttc tgaccgtgta ttaggattag gtcgttcaaa
    16441 attcgtagct agaggtgtaa ttgcaattgc aggatttatt gtgtttgcag tttcaattat
    16501 ctttgctgta cgcacaggaa atttatatgt aagtattttc tggttatcac taggtcttgg
    16561 tggtatcggt atttcaatgg gtatgagttg ggctgcagca actgacttag gacgtaactt
    16621 ctctggtaca gtatcagggt ggatgaactt atggggtaat ataggtgcat taatcagtcc
    16681 gctattagca ggtctattcg tagaacattt gggttggaca atgacattcc aattgttaat
    16741 cgttccagca gtaatcgctg tgattatgtg gttctatgtg aaaccagatc aacctttaat
    16801 tgttagtgat gataaagcaa tagaaaaata atttaaacaa gcagtaagct ttcacatagt
    16861 tggggcttat tgcttttttt gcgttgaaat tgaaactttt taaaacagat atggtttaag
    16921 atgaaaatga agttattgaa atgatatatg taaagaaata aggttttaaa acattagtca
    16981 ggtaacgctt gtaaaagtac atataaattt taactagcgc aaagg
//
LOCUS       DAKTLH010000047        16761 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.047, whole genome shotgun sequence.
ACCESSION   DAKTLH010000047 DAKTLH010000000
VERSION     DAKTLH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16761
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.047"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            325..726
                     /locus_tag="O0394_001299"
     CDS             325..726
                     /locus_tag="O0394_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0499383.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(846..1409)
                     /locus_tag="O0394_001300"
     CDS             complement(846..1409)
                     /locus_tag="O0394_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0499384.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(1406..2359)
                     /locus_tag="O0394_001301"
     CDS             complement(1406..2359)
                     /locus_tag="O0394_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCY0499385.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            2469..3650
                     /locus_tag="O0394_001302"
     CDS             2469..3650
                     /locus_tag="O0394_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0499386.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            3938..6355
                     /locus_tag="O0394_001303"
     CDS             3938..6355
                     /locus_tag="O0394_001303"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCY0499387.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYK
                     EKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYATNPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLAIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            6377..6688
                     /locus_tag="O0394_001304"
     CDS             6377..6688
                     /locus_tag="O0394_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCY0499388.1"
                     /translation="MKSITTDELKNKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
     gene            7014..13574
                     /gene="sasC"
                     /locus_tag="O0394_001305"
     CDS             7014..13574
                     /gene="sasC"
                     /locus_tag="O0394_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HCY0499389.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNV
                     QSDTNQATPVNSQDKDVANNRGLANSAQNTPNQSATTNQATNQALVNHNNGSIVNQAT
                     PTSVQSSTPSAQNNNHTDGNTTATETVSNANNNDVVSNNTALNVPTKTNENGSGGHLT
                     LKEIQEDVRHSSNKPELVAIAEPASNRPKKRSRRAAPADPNATPADPAAAAVGNGGAP
                     VAITAPYTPTTDPNANNAGQNAPNEVLSFDDNGIRPSTNRSVPTVNVVNNLPGFTLIN
                     GGKVGVFSHAMVRTSMFDSGDNKNYQAQGNVIALGRIHGTDTNDHGDFNGIEKALTVN
                     PNSELIFEFNTMTTKNGQGATNVIIKNADTNDTIAEKTVEGGPTLRLFKVPDNVRNLK
                     IQFVPKNDAITDARGIYQLKDGYKYYSFVDSIGLHSGSHVFVERRTMDPTATNNKEFT
                     VTTSLKNNGNSGASLDTNDFVYQVQLPEGVEYVNNSLTKDFPSNNSGVDVNDMNVTYD
                     AANRVITIKSTGGGTANSPARLMPDKILDLRYKLRVNNVPTPRTVTFNETLTYKTYTQ
                     DFINSAAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNGLKRRAQT
                     ILDENRNNVPLNKRVSQAYIDSLTNQMQHTLIRSVDAENAVNKKVDQMEDLVNQNDEL
                     TDEEKQAAIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKK
                     AIRDKATKQREIINATPDVTEDEIQDALNQLATDETDAIDNVTNATTNADVETAKNNG
                     INTIGAVVPQVTHKKAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHAL
                     EQINQATTNADVDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDAT
                     QEERQAAIDKVNAAVTAANTNILNANTNADVEQVKTNAIQGIQAITPATKVKTDAKNA
                     IDKSAETQHNTIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGT
                     QNIVVIQPATQVKTDTRNVVNDKAREAITNINATTGATREEKQEAINRVNTLKNRALT
                     DIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTNATT
                     EEKQVALNQVDQELATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAALAQI
                     NQHYNAKLAEINATPDATNDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAEN
                     NIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAINQ
                     INQNQTNDQVDTTTNQAVNAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDN
                     EKEVASQALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKVKPAAREKIN
                     QKANELRAKINQDKEATAEERQVALDKINEFVNQAMTDITNNRTNQQVDDTTSQALDS
                     IALVTPDHIVRAAARDAVKQQYEAKKREIEQAEHATDEEKQVALNQLANNEKRALQNI
                     DQAIANNDVKRVETNGIATLKGVQPHIVIKPEAQQAIKASAENQVESIKDTPHATVDE
                     LDEANQLISDTLKQAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEE
                     NNKNQLDAIRNTLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNI
                     KVILPKVQVKPAARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQIN
                     HADKTAQVNQDSINAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQ
                     NIAIAQVEKELIKAKQQIASAVTNADVAYLLHDEKNEIREIEPVINRKASAREQLTTL
                     FNDKKQAIEANIQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQTIH
                     DLDVHPIKKPDAEKTINDDLARVTALVQNYRKVSNRNKADALKAITALKLQMDEELKT
                     ARTNADVDAVLKRFNVALSDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKV
                     KVLIDQYVADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPAEATKVSPKEIQPAPKV
                     CTPIKKEETHESRKVEKELPNTGSEGMDLPLKEFALITGAALLARRRTKNEKES"
     gene            complement(13691..14959)
                     /locus_tag="O0394_001306"
     CDS             complement(13691..14959)
                     /locus_tag="O0394_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0499390.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSSVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKHISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHASME"
     gene            15366..>16761
                     /locus_tag="O0394_001307"
     CDS             15366..>16761
                     /locus_tag="O0394_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCY0499391.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNIHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKY"
BASE COUNT         6095 a         2589 c         3062 g         5015 t
ORIGIN      
        1 ttttttgaag aaatatatgt agaattgttg caatttaatg gtaatattga tatattttct
       61 ttgtatataa attataaaat taatatgtaa tagagtgatt tgttttatgt actattatct
      121 tatttctaaa tattaactct attgattatt ggtttttata cttatttaat tttattcaac
      181 tttgacaatt gaatagaaag caagtttatt tacacttgta gttttatgca taagttagca
      241 catacaagtt ttgggattgt tgggatgttt gttaatactt gtatagtagc taaatatgtg
      301 attattaatt gggagatgtt tagcatgaaa aaagtaaata acgacactgt atttggaatt
      361 ttgcaattag aaacactttt gggtgacatt aactcaattt tcagcgagat tgaaagcgaa
      421 tacaaaatgt ctagagaaga aattttaatt ttactaactt tatggcaaaa aggttctatg
      481 acgcttaaag aaatggacag atttgttgaa gttaaaccgt ataagcgtac gagaacgtat
      541 aataatttag ttgaattaga atggatttac aaagagcgtc ctgttgacga tgaaagaaca
      601 gttattattc atttcaatga aaagttacaa caagagaaag tagagttgtt gaatttcatc
      661 agtgatgcga ttgcaagtag agcaacagca atgcaaaata gtttaaacgc aattattgct
      721 gtgtaagttt aatagcataa aaagaggttt tcattaagtt gaaaacctct ttttgttgtt
      781 ggcattaatt tttcaaatgt tgactactca atcctaaatt ataaatagta tagcgcagca
      841 aatgcttaag aaattttttc tatggcacaa atgaatggag catgattacg ttggtttaaa
      901 aattgatatt gcaaaacttg cgcatgcttt tgatccaaag tactcaagta atcaagcaat
      961 gcatgcttct caatttgtcc ttcgctatga ccatgatata taacaagtac aataatacct
     1021 tcaattgaca ttaatgatag caatgaatta atagcttgga ttgtcgtgtc aggctttgtc
     1081 acgatagatt tatcaccttt aggcaaataa cctaggttaa agatggctgc atcaatatga
     1141 cctttatgtg catcatttat atgatgttca atattttcat gtccatcttt tattaaagaa
     1201 acatgattga aatccttaac tttatcacgt gtattttcca aagctaaatc ttgaatgtcg
     1261 aaaccataaa catgtccttc tggtacttgt tcggctaaaa ataaagtgtc attgccgtta
     1321 ccgcaagttg cgtctacaac aatactttct ggtgttatat gttgtttaat aagtgttttt
     1381 gaaaaaggga gtatacgttc taatttcatt gcttcacctt agacttgtaa cgcaagcctt
     1441 gataagaatt tctacgtgct aattcagcat cgatgccatt taatacttcc catttattaa
     1501 cactccacat tggacctacc atgatatcta ttggaccatc accggtaatt cggtgaacga
     1561 tcatttcagg gggaatcact tctaattggt ccacaactag gtttgtgtac tcttcttgag
     1621 tcataaaagt taataaacct ttatcgtatt gttttaccat cggtgtacct tttaacaaat
     1681 gaagtaaatg aattttaata ccttgtacat ccatttgtgc aacttctttg gcagtagcca
     1741 tcatcatgtc atagtcttcg ccaggtaagc cattaatgat gtgtgtacat acattgatat
     1801 tatgcttacg taattttgcc acaccatcat aataagtttt catatcatgg gcacgattga
     1861 ttaaatcaga tgttgactga tggattgttt gtagtcctaa ttcaacccat aagtatgttc
     1921 gttgattcaa atctgctaaa tattcgacaa catcgtctgg tagacagtca ggacgcgtac
     1981 caatagataa tcccacaaca cccggttctt taagtacagg ttcgaatttt tcttttaata
     2041 cttcaaccgg tgcatgtgta tttgtaaatg cctgaaaata agcaatatat tttccttcgt
     2101 gccatttctc atgcatcttt tctttaattt ctttaaattg tactgcgatt gaatctgcac
     2161 gattacctgc aaagtctccg ctacctgcag cagaacaaaa tgtacatcca ccatgtgcta
     2221 cagtgccatc gcggttagga cagtcaaacc cgccatccaa tgcaacttta aatatttttt
     2281 gtccaaattt attttttaaa tggtaattcc atgtgtgata acgtttgttt tcaaaagcgt
     2341 attggaaatg attgcccata tgtcattttc ctttctataa aaaaagagtt ctaagtacag
     2401 attttaacat attttaatgt tatagtgttt attatagttt gacaaaaaag agagaggaac
     2461 tatgaaatat gaatatacct aaatcagtct ggtggctagt aattggcatg gcgttaaata
     2521 ttactggttc cagttttttg tggcctttaa atacaattta tatgaaacaa gaacttggaa
     2581 aaagtttaac tgttgctggt ttagtgctaa tgataaattc atttggcatg gttattggaa
     2641 acttattagg tggttcacta tttgataaat taggtggata caagacgatt ttaattggaa
     2701 ctttcacttg tctttgtagt acaacgctac ttaatttctt tcacgggtgg ccttggtatg
     2761 ctgtatggct tgtaatgtta gggtttggtg gcggaatgat tattcctgcg atatacgcta
     2821 tggctggagc agtgtggcca aatggcggaa gacaaacgtt taatgcgata tacttagcgc
     2881 aaaatattgg tgtggctgtc ggtgctgcaa tgggcggctt tgtcgcagaa tttagcttta
     2941 actatatctt tttagccaat cttattatgt atgttgtgtt tgcgcttgtc gcggtaacgc
     3001 aatttaatat tgaaattaat gcgaaagtta aatatccaac tcatttagat attactggta
     3061 aaaagaataa agcaagattt atttcattag tactaatttg tgcaatgttt gcaatttgtt
     3121 gggttgcata tattcaatgg gagtctacaa tcgcttcatt tacacaatct attaatattt
     3181 caatggcaca atatagtgtt ttatggacaa ttaacggaat aatgatttta gtagcacaac
     3241 cattaattaa accgattctc tatctgttaa aaggaaactt aaagaagcaa atgtttgtcg
     3301 gcatcatcat ttttatgttg tcgttctttg tcacgagttt tgccgaaaac tttacaatat
     3361 ttgttgtcgg tatgattatt ttaacttttg gagaaatgtt tgtatggcca gcagttccaa
     3421 ctatagccaa tcagttagcg ccagatggta agcaaggaca gtaccaaggt tttgtgaatt
     3481 cagctgctac agtaggaaaa gcatttggtc catttcttgg tggtgtatta gttgatgcgt
     3541 ttaatatgcg catgatgttt atcggtatga tgctactact tgtatttgca ttaatattat
     3601 taatggtttt caaggagaat aatacgcaac ctaaaaaaat agatgcataa tgagtaaata
     3661 gaattaacgt tatagacttg aaataaatgt cgttataaca taatattaat ttgtataatt
     3721 taatttcgtt tggagctttt ctacagaaag ctagtgatgc tgagagctag tgttaaggac
     3781 taaatgtaaa tcgtattaat tttaaattga atgaatgaca tctcttacta ttaaaatgag
     3841 tgcacaattt ttgtgaaata gggtggtaac gcggcaaatg tcgtccctat gtaaatagaa
     3901 tagttagagg tgtctttttt attgaatagg aggaaatgtg ttgaattata accacaatca
     3961 aattgaaaag aaatggcaag actattggga cgaaaataaa acatttaaaa caaatgataa
     4021 cttaggtcaa aagaaatttt atgctttaga catgtttcca tatccatcag gtgctggttt
     4081 acatgttgga catcctgagg gctatacagc aacagatatc atttcaagat ataaaagaat
     4141 gcaaggatat aatgtattac atccgatggg gtgggatgca ttcggattac cagcagagca
     4201 atatgcttta gacactggca acgacccacg tgaatttaca aagaaaaata tccaaacttt
     4261 taaacgacaa attaaagaat tagggttcag ttatgattgg gatcgtgaag ttaatacaac
     4321 agatccagaa tactataaat ggacacagtg gattttcata cagttatata acaaaggttt
     4381 agcatacgtt gatgaagttg cagttaactg gtgtccagca ttaggcactg ttttatctaa
     4441 cgaagaagtg attgatggtg tctctgaacg tggtggacat ccagtttatc gtaagccgat
     4501 gaaacaatgg gtacttaaaa tcacagaata tgcagatcaa ttattagcag atttagatga
     4561 tttagattgg cctgagtctt taaaagatat gcagcgcaat tggattggac gttctgaagg
     4621 ggccaaagtt tcatttgatg tagataatac ggaaggaaaa gtagaagtat ttacgactag
     4681 accagataca atctatggtg catcattctt agtcttaagt cctgaacatg cattagttaa
     4741 ttcaattaca acagatgaat ataaagaaaa agtaaaagct tatcaaacag aagcttctaa
     4801 aaagtcagat ttagaacgta cagatttagc aaaagataaa tcaggtgtat ttactggtgc
     4861 atatgcaact aatcctttat ctggtgaaaa agtacaaatt tggattgctg attatgtatt
     4921 atcaacatat ggtactggag caattatggc agtaccagcg catgatgaca gagattatga
     4981 atttgctaaa aagtttgatt tgccaatcat tgaagtcatc gaaggtggaa atgttgaaga
     5041 agcagcatac actggtgaag gtaaacatat taattctggt gaacttgatg gtttagaaaa
     5101 tgaagcggca attactaaag ctattcaatt attagagcaa aaaggtgctg gcgaaaagaa
     5161 agttaattac aaattaagag attggttatt cagtcgtcag cgttattggg gcgaaccaat
     5221 tcctgtcatt cattgggaag atggaacaat gacaactgtt cctgaagaag agctaccatt
     5281 gttgttacct gaaacagatg aaatcaagcc atcagggact ggtgagtctc cactagctaa
     5341 tattgattca tttgtaaatg ttgtagatga aaaaacaggt atgaaaggac gtcgtgaaac
     5401 aaatacaatg ccacaatggg caggtagttg ttggtattat ttacgttaca tcgatcctaa
     5461 aaatgaaaat atgttagcag atcctgaaaa attaaaacat tggttacctg ttgatttata
     5521 tatcggtgga gtagaacatg cggttcttca cttattatat gcaagatttt ggcataaagt
     5581 cctttatgat ttggctatcg tacctactaa agaacctttc caaaaattat ttaaccaagg
     5641 tatgatttta ggagaaggta atgagaagat gagtaaatct aaaggaaatg taatcaatcc
     5701 tgatgatata gtacagtctc atggtgcaga tactttgcgt ctttacgaaa tgtttatggg
     5761 acctttagat gctgcaattg catggagtga aaaaggatta gatgggtctc gtcgattctt
     5821 agatcgcgta tggcgtttaa tggtaaatga agatgggaca ttgagttcaa aaattgtaac
     5881 tacaaataat aaatctttag ataaagttta taaccaaact gttaaaaagg taacagaaga
     5941 ctttgaaaca ttaggattta atactgctat tagtcaatta atggtattta ttaatgagtg
     6001 ttataaagtt gatgaagttt ataaacctta cattgaaggc ttcgttaaaa tgttagcacc
     6061 tattgcacca catatcggtg aagaattatg gtcaaaatta ggacatgaag agtctattac
     6121 gtaccaacct tggccaactt atgacgaagc actacttgta gatgatgaag tagaaatcgt
     6181 tgttcaagtg aatggtaaat tgagagctaa aattaaaatt gctaaagata catcaaaaga
     6241 agaaatgcaa gaaattgcct tatctaatga caatgttaaa gcgagtattg aaggtaaaga
     6301 catcatgaaa gtcatcgctg ttcctcaaaa attagtcaat attgtagcta aataatgttt
     6361 taaggaggac tttgaaatga agtcaattac tacagatgaa ttaaaaaata aacttttaga
     6421 atctaaacca gttcaaattg ttgatgttcg tactgatgaa gaaacagcaa tgggatatat
     6481 tcctaatgca aagttaattc caatggatac cattccggat aatttaaatt catttaataa
     6541 aaatgaaata tattatattg tatgtgctgg tggagttcga agcgctaaag ttgtagaata
     6601 tttagaggca aatggcattg atgccgtaaa tgtcgaaggc ggcatgcacg catggggcga
     6661 tgaaggtttg gaaataaaaa gtatttaaag tagtgacata atttaaaata atattacatt
     6721 tgtaatgaca ccaagtaacg tttcggttgc ttggtgtttt ttggtatgaa ttactttctg
     6781 ttacaaaaca atctaaagcg ttcttgttat gttttattaa gattttaatt acaaaacgga
     6841 aactaaattg taataaaata aaactttatt ttataaaatg atgatgataa aattgagtga
     6901 acttaaaata ttgtacaaaa taatatagct ataaatataa tatgagggag cgtatatttt
     6961 tagcataatt cttaacaaca cagcagagaa cagacaacca ggaggaaaat gaaatgaatt
     7021 tgttaaagaa aaataaatat agtattagga agtataaagt aggcatattc tctactttaa
     7081 tcggaacagt tttattactt tcaaacccaa atggtgcaca agccttaact acggataata
     7141 atgtacaaag cgatactaat caagcaacac ctgtaaattc acaagataaa gatgttgcta
     7201 ataatagagg tttagcaaat agtgcgcaga atacacctaa tcaatctgca acaaccaatc
     7261 aagcaacgaa tcaagcattg gttaatcata ataatggtag tatagtaaat caagctacgc
     7321 caacatcagt gcaatcaagt acgccttcag cacaaaacaa taatcataca gatggcaata
     7381 caacagcaac tgagacagtg tcaaacgcta ataataatga tgtagtgtcg aataataccg
     7441 cattaaatgt accaactaaa acaaatgaaa atggttcagg aggacatcta actttaaagg
     7501 aaattcaaga agatgttcgt cattcttcaa ataaaccaga gctagttgca attgctgaac
     7561 cagcatctaa tagaccgaaa aagagaagta gacgtgcggc accggcagat cctaatgcaa
     7621 ctccagcaga tccagcggct gcagcggtag gaaacggtgg tgcaccagtt gcaattacag
     7681 cgccatatac gccaacaact gatcctaatg ccaataatgc aggacaaaat gcacctaacg
     7741 aagtgctgtc atttgatgac aatggtatta gaccaagtac caaccgttct gtgccaacag
     7801 taaacgttgt taataacttg ccgggcttca cactaatcaa tggtggcaaa gtaggggtgt
     7861 ttagtcatgc aatggtaaga acgagcatgt ttgattcagg agataataag aactatcaag
     7921 cacaaggaaa tgtaattgca ttaggtcgta tacatggaac tgatacgaat gaccatggcg
     7981 attttaatgg tatcgagaaa gcattaacag taaatccgaa ttctgaatta atctttgaat
     8041 ttaatacaat gactactaaa aacggtcaag gcgcaacaaa tgttattatc aaaaatgctg
     8101 atactaatga tacgattgct gaaaagactg ttgaaggcgg tccaactttg cgtttattta
     8161 aagtacctga taatgtgaga aatctcaaaa ttcaatttgt acctaaaaat gacgcaataa
     8221 cagatgcgcg tggcatttat caactaaaag atggttacaa atactatagc tttgttgact
     8281 ctatcggact tcattctggg tcacatgttt ttgttgaaag acgaacaatg gatccaacag
     8341 caacaaataa taaagagttt actgtaacaa catcattaaa gaataatggt aattctggtg
     8401 cttctctaga tacaaatgac tttgtatatc aagttcaatt acctgaaggt gttgaatatg
     8461 tgaacaattc attgactaaa gattttccaa gtaacaattc aggcgttgat gttaatgata
     8521 tgaatgttac atatgatgca gcaaatcgtg tgataacaat taaaagtact ggaggaggta
     8581 cagcaaactc tccggcacga cttatgcctg ataaaatact cgatttaaga tataaattac
     8641 gtgtaaataa tgtgccgaca ccaagaacag taacatttaa cgagacatta acgtataaaa
     8701 catatacaca agatttcatt aattcagctg cagaaagtca tactgtaagt acaaatccat
     8761 atactatcga tatcatcatg aataaagatg cattacaagc cgaagttgac agacgtattc
     8821 aacaagctga ttatacattt gcgtcattag atatctttaa tggtctgaaa cgacgcgcac
     8881 aaacgatttt agatgaaaat cgtaacaatg taccattaaa taaaagagtt tctcaagcat
     8941 atattgattc attaactaat caaatgcaac atacgttaat tcgaagtgtt gatgctgaaa
     9001 atgcagttaa taaaaaagtt gaccaaatgg aagatttagt taatcaaaat gatgaattga
     9061 cagatgaaga aaaacaagca gcaatacaag ttatcgagga acataaaaat gaaataattg
     9121 gtaatattgg tgaccaaacg actgatgatg gcgttactag aatcaaagat caaggtatac
     9181 agaccttaag tggggatact gcaacaccgg ttgttaaacc aaatgctaaa aaagcaatac
     9241 gtgataaagc aacgaaacaa agggaaatta tcaatgcaac accagatgtt actgaagacg
     9301 agattcaaga tgcactaaat caattagcta cggatgaaac agatgctatt gataatgtta
     9361 cgaatgctac tacaaatgct gacgttgaaa cagctaaaaa taatggcatc aatactattg
     9421 gagcagttgt tcctcaagta actcataaaa aagctgcaag agatgcaatt aaccaagcaa
     9481 cagcaacgaa aagacaacaa ataaatagta atagagaagc aactcaggaa gagaaaaatg
     9541 cagcattgaa cgaattaact caagcaacca accatgcttt agaacaaatc aatcaagcaa
     9601 caacaaatgc tgatgttgat aacgccaaag gagatggtct aaatgccatt aatccaattg
     9661 ctcctgtaac tgttgttaag caagctgcaa gggatgccgt atcacatgat gcacaacaac
     9721 atatcgcaga gatcaatgct aatcctgatg cgactcaaga agaaagacaa gcagcaattg
     9781 acaaagtgaa tgctgctgta actgcagcaa acacaaacat tttaaacgct aataccaatg
     9841 ctgatgttga acaagtaaag acaaatgcga ttcaaggaat acaagcaatt acaccagcta
     9901 caaaagtaaa aacagatgca aaaaatgcca tcgataaaag tgcggaaacg caacataata
     9961 cgatatttaa taataatgat gcgacgctcg aagaacaaca agcagcacaa caattacttg
    10021 atcaagctgt agccacagcg aagcaaaata ttaatgcagc agatacgaat caagaagttg
    10081 cacaagcaaa agatcagggc acacaaaata tagtagtgat tcaaccggca acacaagtta
    10141 aaacggatac tcgcaatgtt gtaaatgata aagcgcgaga ggcgataaca aatatcaatg
    10201 ctacaactgg cgcgactcga gaagagaaac aagaagcgat aaatcgtgtc aatacactta
    10261 aaaatagagc attaactgat attggtgtga cgtctactac tgcgatggtc aatagtatta
    10321 gagacgatgc agtcaatcaa atcggcgcag ttcaaccgca tgtaacgaag aaacaaactg
    10381 ctacaggtgt attaaatgat ttagcaactg ctaaaaagca agaaattaat caaaacacaa
    10441 atgcaacaac tgaagaaaag caagtggctt taaatcaagt ggatcaagag ttagcaacgg
    10501 caattaataa tataaatcaa gctgatacaa atgcggaagt agatcaagcg caacaattag
    10561 gtacaaaagc aattaatgcg attcagccaa atattgttaa aaaacctgca gcattagcac
    10621 aaatcaatca gcattataat gctaaattag ctgaaatcaa tgctacacca gatgcaacga
    10681 atgatgagaa aaatgctgcg atcaatactt taaatcaaga cagacaacaa gctattgaaa
    10741 gtattaaaca agctaacaca aatgcagaag tagaccaagc tgcgacagta gcagagaata
    10801 atatcgatgc tgttcaagtt gatgtagtaa aaaaacaagc agcgcgagat aaaatcactg
    10861 ctgaagtggc gaagcgtatt gaagcggtta aacaaacacc taatgcaact gacgaagaaa
    10921 agcaggctgc tgttaatcaa atcaatcaac ttaaagatca agcaattaat caaattaatc
    10981 aaaaccaaac aaatgatcag gtagacacaa ctacaaatca agcggtaaat gctatagata
    11041 atgttgaagc tgaagtagta attaaaccaa aggcaattgc agatattgaa aaagctgtta
    11101 aagaaaagca acagcaaatt gataatagtc ttgattcaac agataatgag aaagaagttg
    11161 cttcacaagc attagctaaa gaaaaagaaa aagcacttgc agctattgac caagctcaaa
    11221 cgaatagtca ggtgaatcaa gcagcaacaa atggtgtatc agcgattaaa attattcaac
    11281 ctgaaacaaa agttaaacca gctgcacgtg aaaaaatcaa tcaaaaagcg aatgaattac
    11341 gtgctaagat taatcaggat aaagaagcaa cagcagaaga aagacaagta gcactagata
    11401 aaatcaatga atttgtaaat caagccatga cagatattac gaataataga acaaatcaac
    11461 aagttgatga tacaacaagt caagcgcttg atagcattgc tttagtgacg cctgaccata
    11521 ttgttagagc agctgctaga gatgcagtta agcaacaata tgaagctaaa aagcgcgaaa
    11581 ttgagcaagc ggaacatgcg actgatgaag aaaaacaagt tgctttaaat caattagcga
    11641 ataatgaaaa acgtgcatta caaaacatcg atcaagcaat agcgaataat gatgtgaaac
    11701 gtgttgaaac aaatggcatt gctacactaa aaggtgtaca acctcatatt gtaattaagc
    11761 ctgaagcaca acaagcaata aaagcaagtg cagaaaatca agtagaatca ataaaagata
    11821 caccacatgc aacagttgat gaattagatg aagcgaatca attaattagc gacacactca
    11881 aacaagcgca acaagaaata gaaaatacaa atcaagatgc tgctgttact gatgttagaa
    11941 atcaaacaat caaggcaata gagcaaataa aacctaaagt aagacgtaaa cgagctgcgc
    12001 ttgatagcat tgaagaaaat aataaaaatc aactcgatgc aatccgaaat acgttggata
    12061 ctactcaaga tgaaagagat gttgctattg atactttaaa taaaattgta aatacaatta
    12121 aaaatgacat tgcacaaaac aaaacgaatg cagaagtgga tcgaactgag actgatggca
    12181 acgacaacat caaagtgatt ttacctaaag ttcaagttaa accagcagcg cgtcaatctg
    12241 ttggtgtaaa agccgaagct caaaatgcac taatcgatca aagcgattta tcaactgaag
    12301 aagaaagact agctgctaaa catttagtag aacaagcact taatcaggct attgatcaga
    12361 tcaatcatgc agataagact gcccaagtta atcaagatag tataaatgct caaaatatta
    12421 tttcaaaaat taaaccagcg acaacagtta aagcaacagc attacaacaa attcaaaata
    12481 tcgctacaaa taaaattaat ttaattaaag caaataacga agcgacagat gaagaacaaa
    12541 atattgcaat agcacaagtt gaaaaagagt taattaaagc taaacaacaa attgctagtg
    12601 cagtgactaa tgcagatgtg gcatatttat tgcatgatga gaaaaacgaa attcgtgaaa
    12661 tcgaacctgt tattaacaga aaggcgtctg ctcgagaaca attgacaaca ttattcaacg
    12721 ataaaaaaca agcaattgaa gcgaatattc aagcaacggt agaagaaaga aatagtatat
    12781 tagcacagtt acaaaatatt tatgacactg ctattggaca aattgatcaa gatcgtagca
    12841 atgcacaagt tgataaaaca gcatcattaa atctacaaac aatacatgat ttagatgtac
    12901 atcctattaa aaagccagat gctgaaaaaa cgattaatga tgatcttgca cgcgtcactg
    12961 ctttagtgca aaattatcga aaagtaagta atcgtaataa ggctgatgca ttaaaagcta
    13021 taactgcttt aaaattacaa atggatgaag aattaaaaac agcacgcact aatgctgatg
    13081 ttgatgcagt tttaaaacga tttaatgttg cattaagcga tatagaagca gtaattactg
    13141 aaaaagaaaa tagcttactg cgaattgata acattgctca acaaacatat gcgaaattca
    13201 aagcgatcgc aacaccagaa caattagcta aagtaaaagt attaattgat caatatgttg
    13261 cagatggcaa tagaatgatt gatgaagatg cgacattaaa tgacatcaaa caacacacgc
    13321 aattcattgt tgatgaaatt ttagcaatta aattaccagc tgaagcgacg aaagtatcac
    13381 caaaagaaat tcagccagct ccaaaagttt gtacgcctat taaaaaagaa gagacacatg
    13441 aatcgcgcaa agttgaaaaa gaacttccaa atacaggttc tgaaggaatg gatttaccat
    13501 tgaaagaatt tgcactgatt acaggtgcgg ctttgttagc tagaagacgt actaaaaacg
    13561 aaaaagaatc ataattaacg aaatgacctt aatataacga cactttttag tgactcagtt
    13621 atattaaggt catttttatt tgaatgaaaa agttgatcta ctaaagagaa tcgaaccaaa
    13681 ttttatacta ttattccatt gatgcatgtg agtaatgtcc ggcatataat ccagcgacat
    13741 gtcctgttac gagtgcactt gtaatattat aaccaccagt ataaccatgt atatctaata
    13801 cttcaccaca taaaaataat cccggaacta atttagacat cattgtttta ggttgaattt
    13861 ctttaagtga cacaccacca cctgtgacaa atgccttatc tataggtaat gtcccgttca
    13921 ccttaaatac aaacccttta aacatattta ctaagtcgtt caattgttga tttgataagt
    13981 gatgtgatgt ggtattttca tcgattcctg cttgttccag catgaacagt aagtagcgct
    14041 cttcaattag accatgcaaa ctgtttttaa tgattttatc tggtgtgtcc gataataatg
    14101 atgtgatgtg ttgttttaat tgttcatggt ttaattcagg aaatgcatcg attgccatag
    14161 aaatgtgctg tgtcttttga tttttttgtt ctttataaac aaactgacta catcttaatg
    14221 cagctggacc actgatacca aaatgagtaa ataacatatc catttgatga ctgatgtgtt
    14281 ttttaccatt tttcttaagt actgacaatt caacatcttt taaacttaaa ccttttagac
    14341 gattggattt gatgaaaggt tcagctgatg taattggaac ttcggtcggg aataactcag
    14401 taatggtatg acctaaatct tgtgcgaact tataaccatc accagttgaa ccagtttgag
    14461 ggacacttgt accacctgta gcaatcacta gcgaatggct ttcataacta ttattttgag
    14521 tatgtacagt gaaagtttgg tctgtattaa cttcgattct actaacagct tcttcttctt
    14581 taatcgttac atgttggcgt tcgatagttg tcactaatgt atcaaccacg tcttgtgctt
    14641 tgttggaaac tggaaacata cgcccgtgat cttcttcttt taatttaaca cccctagact
    14701 caaaaaaatc tatgatggat tcattatcaa aaattgaaaa gggactatat aaaaatttcc
    14761 catttccagg aatgttctta ataatttcag catatggtaa tcgattagtt acgttacatc
    14821 taccgccacc agatattttg agtttacgac ctagaccttt ctttttttca atgagtaaca
    14881 cactgctact ttgttcgctt gcagctactg ccgccattaa gccgctaggt ccgcctccga
    14941 taataattgt ttgatacata tacgcttcct ccaaatttgt tactggacat tcaattgcta
    15001 ttttatagta cttttttaaa ctttgctata tctcttgtgt tatgagatga caattatgtt
    15061 gaatatattt ataatgtgaa cttcataaaa aatgaagatg tatgtgtatg ataagaatga
    15121 tttttctact gttatgcgat gttcatctca aatagtagta taatgtttat atgaaatttc
    15181 gtaatattct aatcaaaatc tagtattcaa tgacaatgtg ttgataattt tgttatgata
    15241 attcattgaa tatacgacaa tgaaataaag ttttgtgata tattcataat aatgaaaaat
    15301 gattttacag agaaacagct tttagtttac aaaactttaa taaacatgag atagggagat
    15361 tcgtaatgag tgaaagtaaa gaaatggtgc gtggaacctt tttaattacc attagtatat
    15421 taattaccaa ggtattgggc gtacttttta tcattccatt caactattta attggtggac
    15481 aagaaaatat ggcgccgttc acatacgctt atgcaccata taatattgca attgcagttg
    15541 cgacagcagg tgtaccatta gcagcttcga aatatgttgc gaaatataat gctattgggg
    15601 catataaagt aagtcagaaa ttttataaat cgagttttat tgtaatgagt attaccggtg
    15661 ttttaggttt cttggtactg tatttcttag caccttatat ttctgaatta acattagcta
    15721 gaaacattca tgataaaaac ggttggtcag tcgatgatat tacttggatt ataagaatca
    15781 ttagtatggt tgttatcttt attcctgtat tagcgacatg gagagggata ttccaaggtt
    15841 ataaatctat ggggccaaca gcagtgtcag aagtaactga gcaaattgca cgcgtgattt
    15901 tcatattaat cggaagttat ttagtactaa atgtctttga tggctctatt ttattagcca
    15961 atggtattgc tacttttgca gcagcagttg gtgcgattat tggtatattc acactctggt
    16021 attactggag aaagcgtaag cataatattg atcgtatggt cgaatctgat tacacagata
    16081 tagatgtgtc atatggcaag atgtataaag aaattattgc ttatagtata ccgttcgtta
    16141 ttgtaagttt gaattatcca ttatttaact tagtagatca atttacacat aacggtgctt
    16201 tatctttagt tggtatacct tcacagttac aagatatatt ctttaatatg ctaaatatgt
    16261 caactaataa aattgtaatg attccgacat cattaagtgc tggttttgca gtgagtttaa
    16321 ttccttatat tacaaaaaca tttgcagaag gtcgcttaca tgaaatgcat catcaaatta
    16381 gaacatctat tggtgtatta atgtttataa ctgttccagc tagtattgga attatggcct
    16441 tggcacaacc attattcaca gttttctatg gttacgatcc aattgtttta ggtcatgacc
    16501 ctaaccatga tggtagtcgc ttattattct attatgcgcc tgtagcaata ctaatttcac
    16561 tattaagtgt aacggcatca atgttacaag gtatagataa acagaaatta acagtttatg
    16621 ttattttagc ttctgtagtt atcaagttag cgttaaacta tccattaatt atgttattcc
    16681 atacacctgg tgcaatttta agtactagta tcgcattatt atttgcaatt ggatgtaatt
    16741 tctatattct taaaaaatat g
//
LOCUS       DAKTLH010000048        16550 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.048, whole genome shotgun sequence.
ACCESSION   DAKTLH010000048 DAKTLH010000000
VERSION     DAKTLH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16550
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.048"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            50..511
                     /gene="tsaE"
                     /locus_tag="O0394_001308"
     CDS             50..511
                     /gene="tsaE"
                     /locus_tag="O0394_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCY0499392.1"
                     /translation="MIKINNLDEMNQFAIFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            492..1154
                     /gene="tsaB"
                     /locus_tag="O0394_001309"
     CDS             492..1154
                     /gene="tsaB"
                     /locus_tag="O0394_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCY0499393.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTVKLQDELQGEVIAQLPNASVMYQLLDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            1127..1591
                     /gene="rimI"
                     /locus_tag="O0394_001310"
     CDS             1127..1591
                     /gene="rimI"
                     /locus_tag="O0394_001310"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCY0499394.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDLERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            1584..2609
                     /gene="tsaD"
                     /locus_tag="O0394_001311"
     CDS             1584..2609
                     /gene="tsaD"
                     /locus_tag="O0394_001311"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCY0499395.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITATINEALGDADVSIEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(2711..4321)
                     /locus_tag="O0394_001312"
     CDS             complement(2711..4321)
                     /locus_tag="O0394_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HCY0499396.1"
                     /translation="MSTNQTFLIFVIAIILLTSVIGIVGRYMSRQRLLKSMETLWQTI
                     SPLEAFIRPNSHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMK
                     LYACLRGMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRQNIL
                     FLCPFLPVISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLK
                     QGYTISKIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLF
                     HSIQKSYTTHMNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELT
                     HPLIADAVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPG
                     IVFTSMANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAAS
                     ESVLSFLHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKAN
                     TRNAIELLKITSFPAKIYERAKDNVPKI"
     gene            4410..4538
                     /locus_tag="O0394_001313"
     CDS             4410..4538
                     /locus_tag="O0394_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499397.1"
                     /translation="MIKTMSIENMRTYQQRVNNLWIKGVDNVIYCGYQVGKQKIND"
     gene            complement(4682..6610)
                     /gene="abc-f"
                     /locus_tag="O0394_001314"
     CDS             complement(4682..6610)
                     /gene="abc-f"
                     /locus_tag="O0394_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HCY0499398.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDCLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLYGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPMSIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLLLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHKKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
     gene            6863..7498
                     /locus_tag="O0394_001315"
     CDS             6863..7498
                     /locus_tag="O0394_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCY0499399.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            7802..8881
                     /locus_tag="O0394_001316"
     CDS             7802..8881
                     /locus_tag="O0394_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HCY0499400.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATDILQIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVHQQSAKVKQQTQIV"
     gene            8941..9165
                     /locus_tag="O0394_001317"
     CDS             8941..9165
                     /locus_tag="O0394_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCY0499401.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            9373..10623
                     /locus_tag="O0394_001318"
     CDS             9373..10623
                     /locus_tag="O0394_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCY0499402.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            10806..11756
                     /locus_tag="O0394_001319"
     CDS             10806..11756
                     /locus_tag="O0394_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0499403.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            11905..13389
                     /locus_tag="O0394_001320"
     CDS             11905..13389
                     /locus_tag="O0394_001320"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCY0499404.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYDEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            13386..14345
                     /locus_tag="O0394_001321"
     CDS             13386..14345
                     /locus_tag="O0394_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCY0499405.1"
                     /translation="MRRLFSIGEALIDFIPNVTNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(14533..14640)
                     /locus_tag="O0394_001322"
     CDS             complement(14533..14640)
                     /locus_tag="O0394_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499406.1"
                     /translation="MHCIAGFLILLTKLNMTRELKNDVTSLCIPIPITT"
     gene            complement(14712..15428)
                     /locus_tag="O0394_001323"
     CDS             complement(14712..15428)
                     /locus_tag="O0394_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0499407.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(15447..>16550)
                     /locus_tag="O0394_001324"
     CDS             complement(15447..>16550)
                     /locus_tag="O0394_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCY0499408.1"
                     /translation="SFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAYITKIS
                     DSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSLF
                     FFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEY
                     TLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMPGLRDYFNKNIVPMKDNLQMNAIK
                     LNGIENLKVREIKGLITAKILRAQEMNFPISIEIPDEVSSINLNMIDLSRSIGIILDN
                     AIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGL
                     STLKEIADNADNVLLDTIIENGFFIQKVEIINN"
BASE COUNT         5631 a         2338 c         2963 g         5618 t
ORIGIN      
        1 atggataatc atttttatga atgaaaagca taatatagga gaatcaacat tgataaagat
       61 aaataattta gatgaaatga atcaatttgc tatattttta gttgagcaat tgaaaagtgg
      121 tgatttgatt ttacttaacg gagatttagg agcaggtaaa acaacgttaa cgcaatttat
      181 aggaaaagct cttggtgtaa gacgtacgat taattccccg acatttaaca tcattaaatc
      241 atataggggt aaaaatttaa aattgcatca tatggattgt tatcgcttag aagattctga
      301 tgaagattta ggatttgatg aatttttcga agatcaggca attactgtta ttgaatggag
      361 tcaatttata aaagatttac ttccagcgac gcatttatct attaacattt caacaatatc
      421 tgaaaataca agacaaattg agttgttcgc gcaaggagaa cattatgaac aaattaagga
      481 ggcaattatc catgaattcg ctgctcattg atacatcgaa tcaaccatta tcagtagcat
      541 tgatgcaaaa cgatgaagtt ttagctgaaa taactacgga ttctaaacaa aatcattcag
      601 tgcaattaat gcctgcgatt agtcagctat ttgagcagag caaaatagct aaacagcaat
      661 tagatgcgat tattgttgct gaaggtcctg gttcatatac tggcttacgt atcggtgtta
      721 ctgttgctaa aacattagcg tatgcgttag atgttaaatt atatggtgtt tcatcattaa
      781 aggcattggc agcaacaatc gatcatactg ataaattatt ggttcctgtg tttgatgcaa
      841 gacgacaagc agtttataca ggcatattcc agtggcaaaa tggtcaatta gtgactattt
      901 tagaagatca atatatgtcg atagaggcat tgcgagcttt tttagaagat ttaaaccaac
      961 catttattta tattgggaaa gatactgtaa aattacaaga tgaattacaa ggtgaagtga
     1021 ttgcgcagct accaaatgca tcagtcatgt atcaattgct agataaacct tcagacgtac
     1081 atacatttac tcctaaatac cataaattag ctgaggcgga acgaaattgg atcaacagtc
     1141 aaaagaacaa ttaaacatta gagagatgac taaagaagat gtgccacaag tctttgatct
     1201 tgagcgtcgg agtttcaatg acagttcttg gacaattgac gcattttatc atgaaataga
     1261 gcaaaataac tttgcgaaat attttgtctt agaatttgaa caacaaatta ttggttattt
     1321 aggtttatgg attgttatcg atcaagctca aattacaaca gttgcaatcg atgataatta
     1381 tagaggctat ggtttaggtc aaatgctttt aaaatacgga ataaactatg caagtcacac
     1441 ttgtgatgtg atgagtttag aagtaagagt taataataaa gtggcacaac atgtatatga
     1501 aaatttagga tttcaatatg gtggtaaacg taaaaattat tatggtgaag gtgaggacgc
     1561 aatggttatg tgggtgaatt taaatgacta aagatatatt aatactagct gttgaaacaa
     1621 gttgtgatga aacaagcgtt agtgttataa aaaatggcag agatatttta tcaaatacag
     1681 ttttaagtca gattgaaagt cataaacgat ttggcggtgt cgttcccgaa gtggcaagta
     1741 gacatcacgt tgaaggtata acagcaacaa taaacgaggc tctaggggat gccgatgtat
     1801 caatagaaga tattgatgcc atagcggtta cagaaggccc tggactaatt ggtgcgttac
     1861 taataggtgt taatgcagcc aaagcattgg catttgctta cgataagcca cttattcctg
     1921 ttcatcatat tgcaggacat atatatgcta atcacataga agagccatta acattcccgc
     1981 taattgcact tattgtttca ggtggacata ctgaattagt ttatatgaag gatcatttat
     2041 catttgaagt cattggtgaa acacgagatg acgcagtagg tgaggcttat gataaagtgg
     2101 cacgaacaat tggtttaaat tatccaggtg gtccacaagt tgatcggttg gctgctgaag
     2161 gtgaagatac ttattcattc cctcgtgttt ggttggataa agatagttat gattttagtt
     2221 ttagtgggtt gaaaagtgcc gtaatcaatc aacttcacaa tcaacgacaa aaaaatattc
     2281 caatcattga agctaacgta gcaacgagct ttcaaaacag tgttgtagag gtgctcacgt
     2341 ttaaagctat tcaagcttgt aaagaatatg gtgttcagcg attaattgtt gctggtggcg
     2401 tggcgagtaa taaaggatta cgtcaatctt tagcggatca atgcaaagtc aatgacattc
     2461 aattaactat cccaagtcct aaattatgca cagataatgc tgcaatgata ggcgttgccg
     2521 gccactattt gtatcagcaa ggtcgatttg ctgatttagc attaaatggg cacagcaata
     2581 tagatttaga agagtattct gcagaataaa ataaacgtca gttattaaca tgattactat
     2641 gaacattatt gaaactgttc atagtcttct gtcatgtgat agctgacgtt tttatgttta
     2701 aagttaaatg ctaaattttc gggacattat cttttgctcg ttcatatatt tttgctggaa
     2761 atgaagtgat ttttaataat tcgatggcat tacgtgtatt tgctttgcca ggcttaattt
     2821 tgtaatcaaa atgtatgtta ttattttcta ttacctcatt gaaatggtaa ttttcataac
     2881 gttgttttaa gagttcagct aactcaatat catgtgttgc tgcaataact ctaaagttag
     2941 atttttcatg taaaaatgat agtactgatt ctgaagcggc aattcgttca gttgtgttgg
     3001 tacctttaaa aatttcatct ataaagcagt atattttttg attatctgga atttcaacaa
     3061 tacgcttaat agattttaat tcagccataa aataactatc gcctgataac acatcatcgg
     3121 catttgccat tgacgtgaac acaattccag gttgatatac aaatttgctt gcggtaactg
     3181 tttgtatagc cgatgcaaga ataatgttta ttgctatgga tttcataaat gttgatttac
     3241 ccgaagcatt agacccagtt aacagtatat tttgtgatag tgagaaatca ttggctactg
     3301 catctgctat gagtggatgt gttaactcag agaatactat gccatcattt gaatcatcaa
     3361 tttgtggttc agtataacat tctagcgttc tacgatacat tgctaatgca taatgattat
     3421 ctaattcagc tatgtaatcg aaacaatttt taagctcatt catatgtgtt gtatagcttt
     3481 tttgaattga atgaaataaa acataatcaa gcatgaaaat aagtttaact agtttaatta
     3541 ccatagctcc gatatcttca tcattaactt cagctaaaac actcgttaaa tgacgtgctg
     3601 ttctaaattg cttaaaattg acttcaggtt gtggagcatg tttaattttt gagatagtat
     3661 agccttgttt taaaacattg gatgcataaa agatagattt taagtcatcc tcatatgtac
     3721 gctttaaagt tgctgataaa atgatattaa ataggcaact cataagaaat aataaaatac
     3781 caacttgtga attaataaat ataactgcaa atgagataac tggtaaaaac gggcatagaa
     3841 acaatatatt ttgacgtttt accggtgtga tttggtctgg aaatgttgga taaacagttt
     3901 taccaatcaa agctaaatga tatgttacgt tttttctaaa ttcagcatta tcattaaata
     3961 aactaagtaa tgatttgttc gtaattgaaa gcattccacg taaacaggca tatagcttca
     4021 tttcaccaat agctgttaaa ttataattca tcttatgaaa gattgcattc atatttaagt
     4081 cggaccaagt tttatcatca actaatgaat gtgattcaaa tttttccttg tagagcttat
     4141 actcatagtc gaaatgtgag ttcggtctga taaaagcttc taatggagaa atcgtttgcc
     4201 ataatgtttc catagatttt aatagacgtt gacgactcat gtatcgtcca acaattccta
     4261 ttacagatgt aagtagaata attgctataa caaatattaa aaatgtttga tttgtgctca
     4321 taagtatccc cttatttatt tttaataata ataaaaatat acttcaatat tattattatt
     4381 tcataaagaa aatgtcttaa caatgaattt tgataaaaac aatgtcaata gagaacatgc
     4441 gtacttatca acaaagagtg aataacttgt ggataaaagg tgttgataac gtcatttatt
     4501 gtggatatca ggtgggaaaa cagaaaataa atgattgatt agtgttaatt tacttgtggg
     4561 taactaggat aagtctgtgg atagctataa tatcagcgtt tttattgtga ataaaaaaat
     4621 taaaatttgt aaaaaaataa ctctaactat agcgatttta cagttagagt tatgtatatt
     4681 tttatagttt ttgttgtaat tcttcccatt tagacatagc gtgttctaat ttttgttcgc
     4741 tatcttgttt ttggatagct aattcgtttg ctttttgtgg attgttatac acttctggtt
     4801 gggttaattg ctcgtctatt tgtaagatag tagtctctaa agtttctatt tcattttcac
     4861 actcggatat ttgtcgttca agtttgcgtt gttcacgtct ttgttctttt tggttatgat
     4921 acgacgattg ttccatagac tttttatgtg cttgtacgtt aactgactgt tcttcttggt
     4981 gcgctttcaa cgcttccgct tcctcaactt tttcaatgta atattgataa tctcctaaat
     5041 acatctttcc gccatcaatt gttaaatcaa ataccttgtt tgctaattgg ttaataaagt
     5101 aacgatcatg ggaaacaaat agtattgttc cttcaaaatg ttggagtgct tgctctaaca
     5161 tttcttttga atctatatcg aggtggttgg taggttcatc taaaataagt acattgtcac
     5221 gttgcaacat aagtagtgct agtagtaaac gtgctttttc accaccagat aaatcattaa
     5281 ttatcttttt aacatcgtct tgtacaaata agaaacgtcc aagaactgct cgaatatctt
     5341 tttcattcat taacggatat tgatcccaca cataatctaa aatcgtttta ctagatttaa
     5401 attctgcttg cttttgatca taataaccaa tttgtaaatt tgcgccgaaa gtaatatcgc
     5461 cattaagcgc tttttgttga ttagcaatag ttttaattaa ggtcgatttt ccaataccat
     5521 ttggcccaat gattgctata tgatcgcctt tagagacctc tatactcata ggtttggtaa
     5581 ttgcagtttg ataaccgatt tctaaatttt ttacatgcat gacgtcatta cctgtattcc
     5641 ggtcaaagcc aaattgaata tttgcacttt tggcatctaa cattggttta tcaatgcgtt
     5701 ccattttttc taaaatctta cgtctacttt ttgccattcc acttgttgaa gcacgggtaa
     5761 tatttttctc aacaaaagtt tctaatcgtt ttatttctgc ttgttgactt tcatattctt
     5821 gcattcgttt ttgataatat aaatcccgtt gctgtataaa ttcctcgtaa ttaccaacat
     5881 agcgtttgac atcacctaaa gccacatcat aaatttgagt aactatttta tctaaaaagt
     5941 aacgatcatg gctgatgatg acgattgcac ctttaaaata acgtagataa tcttcaagcc
     6001 actttgtcgt ttctaaatct aagtggttag taggttcatc taaaagtaat aaatcaggtt
     6061 cgtttaatag catttgagct aaagataaac gtgttttttg gccaccgcta aaatcattga
     6121 taggtttatt gaaatcttct tcactaaaat ttaacccgta aagtactgtt ttaattttac
     6181 tttcatattg atatccttct aattgttcaa attgattcga taaagattca taacgggaca
     6241 tatgtgtttt atatgtatca ctatcgtaat catttgcatg ttttgataaa caatctgttt
     6301 cttctttgat taagctttcc attcgtttaa tatgttcaaa tggttttgac atttcttcaa
     6361 aaactgttgc attagaattt aaagtcattt gttgagttaa ataacctagt tttaagtttt
     6421 taatttttga cacatttcca gaatcatagt tttctacgcc agctataatt ttcatcaatg
     6481 ttgatttacc ggcgccattt cttcctacga taccaattcg ttcaccagtt tttacttcaa
     6541 aatcaacatc agtaaatata tcttcaccat cgaacgattt tgatatatga ttgagttgta
     6601 aaagtatcat tcgatcttca cctttcgtct ttctcaaatt ttattaactt atgaaaaaag
     6661 tcgtaattat tacattaata accattttac agtataaaac gccgtcttga aactaagata
     6721 tttattttta aaattcaata atgtataata tttaggtatt cattgtaatc aattatttgt
     6781 tttttaataa tctaacaatt acgctctaac tcgtttgaat acatagatat aattcattca
     6841 aacacgaagg aggaaatagc gaatgagtga ccaagttaaa attcctcgag caactttaaa
     6901 acgtttgccg ttatattata gatttgtcag ttcattaaaa tctaaaggta tagatcgtgt
     6961 aaattcaaaa gcgattagcg atgcgttaca aattgactcg gcaacaattc gtcgtgactt
     7021 ttcatatttt ggcgaattag gtaaaaaagg gtacggatat aatatagata gtttattgga
     7081 tttctttaaa tctgaactaa gcgagagtga catgatcaaa atcgcaattg tcggagttgg
     7141 gaacctaggg aaagctttgc tcacatataa cttttcaata catgacgata tgacgattac
     7201 agaagcgttt gacgtaaaag aagatgttat tggccagaaa atagggaacg ttattgttaa
     7261 agataacgat gaattaataa caacattgaa gaaggaagaa atagatgttg tgattctaac
     7321 tacaccagaa agagttgcac agaaagttgc agatgaactc gtccaagctg gtgtgaaagg
     7381 tattttaaac ttcactcctg gtagaattaa tacgccttca gatgtgcaag tacatcaaat
     7441 tgacttaggt atagaattac agtcattatt attctttatg aaaaattaca gtgaataaat
     7501 gtgatgttag ctttgaatga ttaaccttgt tatatcttta attaattata gaacagtctg
     7561 ggactaagta tgtctcaggc tttttctgtt atgtttgaaa atgaaaggaa aaactgttgt
     7621 gttgataaat atagtatagg ttaataaatt aacacgtaga gatataatga tgaatcgtaa
     7681 ttaatcgcct tccttatatg aatataatat aaatatcaaa aatattgtgt ataaggttga
     7741 aaacaagaaa atcatcacct ataatacata ctaaacttat cggaaatgag aggttaagat
     7801 tatgtggatg atttttagtg gcttaattgt cgggggactc ttaggttttg tcatgcaaag
     7861 aacaagattt tgtttaacag gtggctttcg agatatgtat gtgcaaaaga ataataagat
     7921 gttctatgca ttattaatcg ctattactat tcaaagtata ggattattga ttttgacggc
     7981 aacagatatt ttacaaattc ctgcacatag ttttccaata ttgggaacaa ttataggttc
     8041 ttttattttt ggaattggaa tagtattggc tggaggatgt gcaacaggta cttggtatcg
     8101 cgctggtgaa gggctaattg gtagttggat tgcattagta ttatatgctg ttactgcagc
     8161 aatcactaaa acagggattt taaagccagt aatggataaa attaatcaac caacgaatgt
     8221 aaatagtgat atgtctcaaa caactggcat tccgttttgg ggattagtcg ttatattaac
     8281 tataatcacc atttttctag ttgtaagaac actcaataat aagaaagttc gagttgctgt
     8341 tccaaaacta aagcaacgtt atacaggtat tagatattac cttttcgaaa aacgatacca
     8401 tccatttatt gcagcaattg taattggact tatcgcactc ttagcttggc caatgagtgc
     8461 atcaactgga agaaatgacg gtttaggtat aacaacgcct tcagcaaatt tagtacactt
     8521 tttgattaca ggtgaaacta aatttattga ttggggtgtc tttttagttc taggaatttt
     8581 cattggttca tatattgcag ctagaggatc aagagaattt aaatggcgat tgccagacaa
     8641 gattacaata cgaaacagtg ccattggtgg catatgtatg ggatttggtg cgtcagttgc
     8701 tggtggttgt tctatcggta acggtttggt tgaaacggca acgatgactt ggcaaggatg
     8761 gattgcgcta gcatcgatga tagttggtgt atggacaatg agtcatttta tctttgttcg
     8821 tccaatgaaa aaagtacacc aacaatctgc aaaggttaaa cagcaaacgc aaatagtata
     8881 gaagattatt atgcaaatga tgttgatcaa ataaaagtga ttggaaaagg agaaataatt
     8941 atgatacacg aattaggtac agtaggaatg gtatgtccat ttccgttaat tgaagcgcaa
     9001 aagaaaatgg caacattgca atctggagat gaattaaaaa ttgattttga ttgcacgcaa
     9061 gcgacggaag ccattccaaa ttgggctgca gaaaatggtt atcctgtaac aaactatgaa
     9121 caaattgata atgcttcatg gacaattaca attcaaaaag tttaacgtta tcattttaac
     9181 aataaaatag atattagatt ctatggctac ttccgctaat ttaaaagtga gtaagtagtc
     9241 ttttttgttt tagttcatga aatcattttt atatagtgtg gcacatttta ttccaaaaga
     9301 tgtaataaaa cttaacgcat ttttgctttt tataaattgt cagattatta tgaaaaaaag
     9361 ggagtggtaa gtatgaatct taacgatacg atatttatgt ttttgtgtac attattagtt
     9421 tggttaatga caccaggatt aagtttattt tatggtgggt tagttcaatc taaaaatgcg
     9481 cttaatactg tcatgcaaag tatggcagca attgtgcttg ttacatttgt atggataaca
     9541 gttggtttta caattagttt tgggaatggg aatttatggt tcggaaattg ggaatatact
     9601 tttcttaatc atgtaggttt tgcgactcaa gaagatatta gcccacatat tcctttcgct
     9661 ttgtttatgt tatttcaaat gatgttttgt acgattgcaa tttctatttt atctggttca
     9721 atcgctgaga aaatgaagtt tattccttat ttattattcg tagtaatatg gactgctctt
     9781 gtatacagtc cagtagcaca ttgggtttgg ggcggcggtt ggattaacaa actcggtgta
     9841 ttagatttcg ctggaggtac ggttgttcat attacatcag gtgtttctgg tttagtatta
     9901 gctattatga ttggaaaagg aaacaaacat tctgaatcaa caccacataa tcttatcatt
     9961 acgttgattg gcggtatatt cgtgtggatt ggttggtatg gatttaatgt aggtagtgct
    10021 tttacatttg ataatattgc gatgcttgca tttacaaata ctgtcatttc agccagtgca
    10081 ggtgctatag gttggttaat tttagaatat atttttaaaa agacgacaag tttacttgga
    10141 cttttactcg gtgcattagc aggattagtt gtcattactc ctgcagcagg atatgtaaca
    10201 tatcttagtg caacaataat ggctttaata ggaggtatct gttgttatat tgtcattaat
    10261 tacatcaagg taaaactaaa atatcatgat gcattagatg catttggtat tcatggtgtt
    10321 ggtggtatta ttggtgctgt tttaacagca gttttccaaa gtaaaaaagc caatcctgac
    10381 attgagaatg gctttattta tactggtgac atacatatta tacttgtaca aatattatgt
    10441 gtaacagcag ttgtaatttt tagtatagtc atgacgttta ttattgcgaa agtaattaaa
    10501 ttaattacac cattatctgt tacggaacaa gaaacgaata taggattaga caagattgtt
    10561 cacggtgaac atgcttactt tgaaggtgag ctaaatagat tcaataaaca tattcgatat
    10621 tagaatatat ttacatagaa tattcattgt cctgacattt aactaaaggt tgatgttggg
    10681 acattttgtt atacaaaagt tttattttga aatcttttta tgaaagaagc agaaatatta
    10741 tttaaagcgg ttacacatat gctaaaataa ggctaagtgt cacaaataat gataggtgaa
    10801 taagtatgaa aaatatatct gatattgcca aattggcagg cgtttcaaaa agtacagtat
    10861 ctagattttt aaataatgga tctgtcagta aaaaaacaag tgaaaaatta acaagaatta
    10921 tagcagaaca tgactatcaa ccgaatcaat ttgctcaaag tttaagagcg agacaaacac
    10981 accttattgg tgccattata cctagaatga attcatatgc agtagatgaa acaatcaaag
    11041 gattggcaaa acaatgccaa aaatatgaat cacaattaat tttaaattac acaggtttaa
    11101 atatcgaagc agaaatacaa gcgcttgaaa cattagcacg cagtaaagta gatggtattg
    11161 ttttaatggc tacagacata acagagagac atattgaagt cattaataaa atgaatgtac
    11221 caatcgttat tgttggtcaa caacatgaac aacttcatag tattgtgcat gatgattata
    11281 aagcaggtca aattataggc gaatggattg gtcaacaggg atatcaacaa gttgaagtgt
    11341 ttagtgtaag tgaaaaagat attgcagttg gtatacatag aaaacgtggt ttacttgacc
    11401 agttagctaa ataccaaatt aaacctaata tacatgaaac aaattttact tatgtggaag
    11461 cacaaaaaga tgttgcaaat gttttggaaa atgtggagca agtagatgcg gttgttggag
    11521 caactgatac gattgcatta gctgcctata aatattattc tgataaaaaa gatgttatga
    11581 aaccacatca aatatatggt tttggtggtg acccaatgac acaattagtg tctccatcga
    11641 taaaaacaat tcattataat tattttgaag ctggccaatg cgcgatggaa gagatacaac
    11701 agatgcttaa aaagcaagat atgccatata gcgtcacagt agatgttaat atttagacgc
    11761 tgtatttttt aaaataaatg tggaaccgat accatataac tataaatgga taggttaaaa
    11821 gttaaagaac gtaggtaaaa tttgctataa tagaatataa attgttatta gcataaatta
    11881 taaaaggagg actgggtaaa tattatgacc gaatggacta gagaagaacg ttatcaacga
    11941 atcgaggacg ttgatactga gtattttaaa acattaaaac aacaagttga tcaatcaaaa
    12001 tttcgtcaac aatttcatat acaaccagaa acaggcttat taaatgaccc caacggactt
    12061 attttttata aagggaagta ttatgtttca catcaatggt tcccattagg cgcagtacat
    12121 ggcttaaagt attggtataa ctacacgagt gatgacttaa taaactttaa agctgaaggg
    12181 ccaattttaa atccagatac taaatatgac agccatggtg tatatagcgg tagcgctttt
    12241 gaatataacg ggcatttata ttatatgtac acaggaaatc atcgagataa tcattggcaa
    12301 cgacatgcga gtcagatgat cgcacgattg aaagaagacg gttcagttga aaagtttcca
    12361 aagccagtaa ttagccaaca accagaagga tatacgagtc attttagaga tcctaaagtt
    12421 tttaaatatg atgagaaata ttatgcaatc attggtgcac aaaataatga tcagcaaggt
    12481 cgattattac tttataatac tgaagatata attaattggc attatttagg tgaaataaat
    12541 acagagttgg atgattttgg atatatgtgg gaatgcccag attactttaa tgtagataat
    12601 caagatgtca tacttatttg tccacaaggt attgaaccaa aaggcgatca gttcaaaaat
    12661 atttatcaaa gtggttatat acttggaaag tttgatattg aaaagttaac atatgaacat
    12721 gaaaattttg tcgagcttga taatggtttt gatttctatg cacctcaaac atttttagat
    12781 gaaaaaggcc gacgagtact aattggatgg atggggttac cggaaatcga atatcctact
    12841 gataatgaag gatgggccca ttgcttaaca attcctcgtg tattaaatgt agaaaatggt
    12901 caacttaagc aacgtccata tccagcgttg gaaaaattac gtcacaataa agagacagca
    12961 ttaggctacg caaataaatt tactcgaaaa ttacatccgt atgaaggtaa acagtatgaa
    13021 ttaatcatag atattttgga taatgatgct accgaagtgt actttgaatt acgtacatct
    13081 aagacttctt caacattaat tgcttataac aagcgtgaaa ataaaataac attagatcgc
    13141 agcgacagtg gtttattgcc gacaaatgtt gaaggtacga cgcgtagtac gatattagac
    13201 acgccattaa agcaattaca aatttttgtt gatacatcta gtatagaaat tttctgtaat
    13261 gatggagaac gtgttttgac atctcgaatt ttcccgacag aagacgcact aggtattaaa
    13321 acatcaacag aatcagggca agtatattta caatttacta aatatgattt aaaggatgaa
    13381 cataaatgag acgtctattt tcaataggag aagcgttaat tgattttatt ccaaatgtta
    13441 caaatgcgaa tttaaaagat gttcaaacat ttacgaagca aattggtggc gcgccatgca
    13501 atgtagcttg tacagttcaa aagttaggac aacaagcgta tatgattaca caattaggta
    13561 atgatgcatt tggagatagc attattgaaa cgatatcaag tattggtgta gatgtatcaa
    13621 aagtgtatcg cacgaatgaa gcaaacactg cgttagcttt tgtgagtttg actgaagcgg
    13681 gcgagcgaga tttttctttt tatcgtaagc cctctgctga tatgttattt gaaccaagtt
    13741 ttgtgaatga tattgatgtg aatgaaaatg atgtagtaca tttttgttct gttgatttgg
    13801 tcgatagtcc gatgagagat gcacattatc aattgattac caaaacatta aatgcaaatg
    13861 gtactgtcgt atttgatcct aatgtaagat tgccattatg ggataacgct gaagatttaa
    13921 gacaaactat tcataccttt ttgccattgg cgcatattgt aaaagtttca gatgaagaat
    13981 tagaatttat tacaggtatt catgatgaaa atgaagccat tcaatcatta tttacaggta
    14041 atgttactgt agtcatttat acgaagggag cagatggtgc tgccgtatat ttgaaaaatg
    14101 gtattaatca ttatcatagt ggctataagg taaaacctgt tgatacaact ggggcagggg
    14161 atgcatttat tggtgcagtt attagcagga ttttagcaac cgatgtgtta aatttaactc
    14221 agttatttga aaatgaaggt gaggaaattt tagcgtttag caatcgcgtt gctgcaatag
    14281 tgacaacgaa gtatggtgct attaatagtt tgccaacatt acaagaggtt gaacaagcat
    14341 tttaaataat tcatttataa aacagtgtag gaaacaagtt gaatcttgtg ctacactgtt
    14401 ttttaaaaca aaataagatt cacggagtag gaaattatag cattttattt atggaaactg
    14461 tcaatttcaa atttagtata ttttgttaaa aattgcgcca taggattgta gagtgaaata
    14521 gaaattgata aattatgtag taataggaat aggtatacaa agagacgtta catcattttt
    14581 taattcacgc gtcatattta attttgttaa taaaattaaa aagccagcta tacagtgcat
    14641 ttgctagtta tcttgttaaa atccaacaag atttacaatt gaatacgccg ttaactgact
    14701 ttattatctt attatatttt tttaacgttt ctcaccgatg catagcagtg ttctttattt
    14761 ttaaaataga caattcgctc tttcgaatct atagattcaa tattatggcg attgacgaca
    14821 aagctattat gacatctaaa gaaacgatca tctaattgac tcagttcttt taaattacca
    14881 taaaattcaa tttgacggtt atctaaatgg gcaatgagtc tgtgagattt tgttgatgat
    14941 tcaaaaaaca taatatcatc atattgaaca tacactgaat tactgccacg ttttaattca
    15001 atcgtttcaa cgctattatc tttagacaac aattgtaagc gtgtatgtgc agtttctaaa
    15061 cagtctataa ttcgagttct taattcagct ggatcatctt taaaaataaa atccatcgct
    15121 gcaactttgt agacaaatgt taaataggta agttcactgt gactcgtaac gaaaataatg
    15181 ttaccaactg ggtcatgctt acgaatttca ctgcctaatt tgataccatt aatatcagtt
    15241 gaaagttgaa tatctaaaaa gtaacagcct atgtcattca tatttttagc ttgctcaagc
    15301 acctcataag gattatcagt tgcgagggca atttccatag gcttttcttc tatcattata
    15361 taatttttaa taatggtaac catgttttct ctttgttttg gatcgtcttc gcaaatgaaa
    15421 attttcatac attcacatcc ttatggctag ttgttaataa tttcaacttt ttgaataaag
    15481 aaaccatttt cgataattgt atctaataag acattgtctg cattatcagc aatttctttt
    15541 aaagttgata gacctaaacc acgaccttca cctttagtag aaaaactttc ttggaacaat
    15601 tcatgaatgc gtggtatatc atcagcgcat ttattcataa caataaacgt tactgaattt
    15661 tcactttcaa taaatgcaac gcgaatgata gggtcatcaa tttcagttga tgcctcaatt
    15721 gcattatcaa gaataatacc aatactgcga cttaaatcga tcatattcaa gttaatgcta
    15781 cttacttcat cgggtatttc gatactaatc ggaaaattca tttcttgtgc acgtaaaatt
    15841 ttcgcagtaa ttaagccttt aatttcacgt actttaagat tctcgatacc atttaatttt
    15901 atagcattca tttgtaaatt gtctttcata ggtacaatat ttttattgaa ataatcacgt
    15961 aggccaggca tgtcatcttc tcgaatgtat tctgaaagtg tcgttaagat attgacataa
    16021 tcatgacgga acttgcgcat ttcgttgttg atagcttcaa tcttcaatgt atattcataa
    16081 taggtttcaa tttcttcttg attacgttta tatttcatct ctttaaggag aaattgagaa
    16141 ataacaaatg ttaatatact taaaaatata gtgataccaa taaaaataaa agaatactgc
    16201 cttattactt tagcttcatc cgagtttatt tgtgaataaa agaaaaataa tgaaaaagta
    16261 agcagtaaga tagtcgaaat aactattaaa aatcctttgt ttagtattag atatggtgtg
    16321 ctaatttttt tgagaactct atttattata tatgagaata gtatactaat agtcacataa
    16381 actacaaaaa agctagggaa tattacaaat atactatcag aaattttggt gatatatgca
    16441 tatataacta tatacatgta gttagcacag tatagaataa tctgcgaagt cataatcaac
    16501 aaaatagaat accatttgat tttgacaaaa tacattataa taataaatga
//
LOCUS       DAKTLH010000049        16378 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.049, whole genome shotgun sequence.
ACCESSION   DAKTLH010000049 DAKTLH010000000
VERSION     DAKTLH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16378
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.049"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            225..1754
                     /gene="aur"
                     /locus_tag="O0394_001325"
     CDS             225..1754
                     /gene="aur"
                     /locus_tag="O0394_001325"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HCY0499409.1"
                     /translation="MRKFSRYAFTSMAALTLLSTLSPAALAIDSKNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            2121..2570
                     /locus_tag="O0394_001326"
     CDS             2121..2570
                     /locus_tag="O0394_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCY0499410.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            2573..2740
                     /locus_tag="O0394_001327"
     CDS             2573..2740
                     /locus_tag="O0394_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499411.1"
                     /translation="MYQMNNKNLFRITCTQVSYQILTCVRVFLWKERIHSHSIDCIKN
                     FIQHVFMGVFE"
     gene            2917..4152
                     /gene="arcA"
                     /locus_tag="O0394_001328"
     CDS             2917..4152
                     /gene="arcA"
                     /locus_tag="O0394_001328"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCY0499412.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            4185..5195
                     /gene="argF"
                     /locus_tag="O0394_001329"
     CDS             4185..5195
                     /gene="argF"
                     /locus_tag="O0394_001329"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCY0499413.1"
                     /translation="MTEIQKTYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            5286..6716
                     /gene="arcD"
                     /locus_tag="O0394_001330"
     CDS             5286..6716
                     /gene="arcD"
                     /locus_tag="O0394_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCY0499414.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSVIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFSMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYTIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            6733..7674
                     /gene="arcC"
                     /locus_tag="O0394_001331"
     CDS             6733..7674
                     /gene="arcC"
                     /locus_tag="O0394_001331"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCY0499415.1"
                     /translation="MKEKIVIALGGNAIQTTEATAEAQQTAIRCAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFDNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFVEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            7773..8477
                     /locus_tag="O0394_001332"
     CDS             7773..8477
                     /locus_tag="O0394_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCY0499416.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLICHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
     gene            8827..11526
                     /gene="clfB"
                     /locus_tag="O0394_001333"
     CDS             8827..11526
                     /gene="clfB"
                     /locus_tag="O0394_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HCY0499417.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTTKPMST
                     QTSNTTTTEPASTNETPQPTAIKNQATAAKMQDQTVPQEANSQVDNKTTNDANSIATN
                     SELKNSQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTASNFKLEKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNNKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDISKLSDSYYADP
                     NDSNLKEVTDQFKNRIYYEHPNVASIKFGDITKTYVVLVEGHYDNTGKNLKTQVIQEN
                     VDPVTNRDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPEPSP
                     DPDPDSDSDSDSGSDSDSGSDSDSESDSDSDSDSDSDSDSDSESDSDSESDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSESDSESDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSRVTPPNNEQKAPSNPKGEVNHSNKVSKQHK
                     TDALPETGDKSENTNATLFGAMMALLGSLLLFRKRKQDHKEKA"
     gene            11642..12403
                     /locus_tag="O0394_001334"
     CDS             11642..12403
                     /locus_tag="O0394_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCY0499418.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNSDTTVKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRINVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            12648..13103
                     /locus_tag="O0394_001335"
     CDS             12648..13103
                     /locus_tag="O0394_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCY0499419.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(13206..13316)
                     /locus_tag="O0394_001336"
     CDS             complement(13206..13316)
                     /locus_tag="O0394_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499420.1"
                     /translation="MLGAWLTRSWYFLNAKYKPPTNVKITANFFIIQTPF"
     gene            complement(13386..14810)
                     /locus_tag="O0394_001337"
     CDS             complement(13386..14810)
                     /locus_tag="O0394_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCY0499421.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLKRLLKKEKDEKSAEVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            15008..15208
                     /locus_tag="O0394_001338"
     CDS             15008..15208
                     /locus_tag="O0394_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499422.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQDKEQKDNKG"
     gene            15234..15899
                     /gene="nsaR"
                     /locus_tag="O0394_001339"
     CDS             15234..15899
                     /gene="nsaR"
                     /locus_tag="O0394_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HCY0499423.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQNQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            15910..>16378
                     /gene="nsaS"
                     /locus_tag="O0394_001340"
     CDS             15910..>16378
                     /gene="nsaS"
                     /locus_tag="O0394_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HCY0499424.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISIN"
BASE COUNT         5931 a         2427 c         3034 g         4986 t
ORIGIN      
        1 attttcacaa aaataaagcc ttgataaatt ttaaaatata attaagctca atttttaaaa
       61 ttttatttag ctacagataa catttttaaa aaagaaaaga atcaataaat aaaatcaacg
      121 aacaaaaagt atagaaataa atagaaataa tcatttactt ttctgaaaaa ttaaattaat
      181 attttattta taagctgttt ttaagatttc aggaggaatg aaatgtgagg aaattttcaa
      241 gatatgcatt tacaagtatg gcagcattaa ccttgttgag cactttatca ccagcagcat
      301 tagcgattga ttcaaaaaat aaaccagcta attctgatat taaatttgag gtgactcaaa
      361 agagtgatgc ggtcaaagca ttaaaagaat tgcctaaatc cgaaaatgta aaaaatattt
      421 atcaagatta cgctgttact gatgtaaaaa ctgataaaaa aggatttacg cattatacat
      481 tgcaaccgag tgttgatggt gttcatgcac ctgacaaaga agtgaaagta cacgcagaca
      541 aatcaggaaa agtcgtttta atcaatgggg atactgatgc gaagaaagta aagccaacga
      601 ataaagtgac attaagtaaa gatgacgcag ccgacaaagc atttaaagca gttaagattg
      661 ataagaataa agcgaaaaat cttaaagata aagtcattaa agaaaacaaa gttgaaatcg
      721 atggtgacag taataaatac gtttataatg ttgagttaat tacagtgaca ccagaaattt
      781 cacattggaa agttaaaatt gatgctcaaa ctggcgaaat tttagaaaaa atgaacttag
      841 ttaaagaagc tgcagaaact ggtaaaggaa aaggtgtact tggcgataca aaagatatca
      901 atatcaatag tattgacggt ggatttagcc tagaagattt aacgcatcaa ggtaaattat
      961 cagcatttag ctttaatgat caaacaggtc aagcaacatt gattactaat gaagatgaaa
     1021 acttcgtaaa agatgagcaa cgtgctggcg tagatgcaaa ttattacgct aaacaaacat
     1081 atgattatta caaagacaca tttggtcgtg aatcatatga caaccaaggt agtccaattg
     1141 tttcattaac gcatgttaat aactacggtg gtcaagataa cagaaataat gccgcatgga
     1201 tcggtgacaa aatgatctat ggtgatggtg atggtcgcac attcacaagt ttatcgggtg
     1261 caaatgacgt agtagcacac gaattaacac acggtgtgac acaagagaca gcgaacttag
     1321 aatataagga ccagtcaggc gctctaaatg aaagcttttc agatgttttt ggatactttg
     1381 tagatgacga ggatttctta atgggtgaag atgtctacac acctggaaaa gagggagacg
     1441 ctttacgcag catgtcaaac ccagaacaat ttggtcaacc agctcatatg aaagactatg
     1501 tattcactga aaaagataat ggtggcgtac atacgaattc tggaattcca aataaagcag
     1561 cttataacgt gattcaagca atagggaaat ctaaatcaga acaaatttac taccgagcat
     1621 taacggaata cttaacaagt aattcaaact tcaaagattg taaagatgca ttataccaag
     1681 cggctaaaga tttatatgac gagcaaacag ctgaacaggt gtatgaagca tggaatgaag
     1741 taggcgtgga gtaaaaatat ataaacaaga agaagtaatg ttaaacactt ataaataatt
     1801 aaattttaaa tacagcttat atatggggta tacctacatt gataattgac tttgtaggta
     1861 taccctctct ttgtaattag atgagaggag tgtgagggtt gtctgccgaa agactactcg
     1921 gcagtctaaa atcattacaa gtagtagata tgtgataatt aaatgctgac ttagaataca
     1981 aaattcattt taaaagttgt cacaaaaaat ttacatgtat ttttattata ttttgcaaaa
     2041 caaagtgtta aattataaat gaaacatgca tgaatttatt ttttaataca agaaacgtaa
     2101 ctaccaaagg agtttacaat atgaagaaaa gtaaacgatt agaaattgtt tctacaatag
     2161 ttaaaaagca taagatttat aaaaaagaac aaatcatttc atatattgaa gaatattttg
     2221 gtgtaagata tagtgcaaca acaattgcta aagacttgaa ggaactaaat atatatcgtg
     2281 tccctatcga ttgtgagaca tggatttata aagctattaa taatcaaaca gaacaagaga
     2341 tgagagaaaa gtttagacac tattgtgaac atgaagttct aagttcaatc atcaatggtt
     2401 catacattat cgtcaaaacc tcacctggtt tcgcccaagg cataaactat tttatcgatc
     2461 agctaaatat agaagagata ttaggtacgg tgagtggaaa tgacactaca ttaatcttaa
     2521 ctgcctcaaa tgatatggca gaatacgtat atgcaaaatt atttaaatag acatgtatca
     2581 aatgaataat aaaaatttgt ttcgtatcac gtgtactcaa gttagttacc aaatattaac
     2641 ttgtgtacgc gtttttttat ggaaagaaag aattcatagt cattcaattg actgtataaa
     2701 aaactttata caacatgttt ttatgggtgt ttttgaataa aaaatgtata ttttgaccca
     2761 aaatgccttt atttatgtat aaaaatccat tattatgtat tgtataacaa aaagatatga
     2821 aattttcgac tttctttatg tgaatataat cacatgtaag cgtttgaaga ttgtctatac
     2881 tctaaatgaa ttcaaagata aaaggaggaa atagacatga cagatggtcc aattaaagta
     2941 aatagcgaaa ttggagcttt aaaaactgtg ttacttaagc gtcctggaaa agaattagaa
     3001 aatttagtac ctgattattt agatggatta ctatttgatg atattccata tttagaagta
     3061 gctcaaaaag agcatgacca ttttgcgcag gtgctaagag aagagggtgt tgaagtactt
     3121 taccttgaga agttagcagc tgaaagtatt gaaaatcctc aagtaagaag tgaatttatt
     3181 gatgatgtat tagcagagtc taaaaaaaca atattaggtc atgaagaaga aattaaggca
     3241 ttatttgcga cactttctaa tcaagaactt gtagataaaa taatgtcagg ggtacgtaag
     3301 gaagaaatta atccgaaatg tacacatcta gtagagtata tggatgataa gtatccattc
     3361 tatttagatc caatgccaaa cctttatttt actagagatc cacaagcctc aataggacac
     3421 ggtataacaa tcaatcggat gttctggaga gcacgacgac gagaatcaat atttattcaa
     3481 tatattgtaa agcatcatcc tagatttaaa gatgcgaata ttccaatctg gttagatcga
     3541 gattgcccat tcaatattga aggcggcgat gaacttgttt tatctaaaga tgtcttggct
     3601 ataggcgttt cagaacgtac atctgcacaa gctattgaaa agttagcgcg acgtattttt
     3661 gaaaatccgc aggcgacgtt taaaaaagta gtagcaattg aaattccaac tagtcgaact
     3721 tttatgcact tagatacagt atttacaatg atagattatg acaaatttac aatgcattca
     3781 gccattttaa aggcagaagg caatatgaat atatttatta ttgaatatga tgacgtaaat
     3841 aaagatattg ccatcaaaca atctagtcat ttaaaagata ctttagaaga cgtactaggt
     3901 atagatgata tccaattcat tccaacagga aatggtgatg tcattgatgg tgctagagag
     3961 caatggaatg atggctcaaa tacattatgt ataagaccag gcgttgtagt gacttacgat
     4021 agaaactatg tatcgaatga tttattgaga caaaaaggca ttaaagtcat tgaaatatct
     4081 ggtagcgagt tggtacgtgg acgtgggggc cctagatgta tgagtcaacc attattcaga
     4141 gaagacattt aacgtaataa attatagaaa cgagggtgaa aataatgaca gaaattcaaa
     4201 aaacgtatga tttaaaaggc agatcattat taaaagaaag tgattttacc aaagcagaat
     4261 tcgaaggact tattgatttt gcaattacat taaaagagta taagaaaaac ggtattaagc
     4321 atcactactt atctggaaaa aatattgcac tactattcga aaagaattcg acgagaacgc
     4381 gtgctgcgtt tacagttgcg tctattgatt taggtgcgca tccagaattt ttaggaaaaa
     4441 atgatattca attaggcaaa aaagaatctg tagaggatac tgcgaaagta ttaggtagaa
     4501 tgttcgatgg tattgaattc cgtggttttt cacaacaagc tgttgaagat ttagcgaagt
     4561 tctctggtgt accggtgtgg aatggattaa cagacgattg gcatcctaca caaatgttag
     4621 ctgattttat gacaataaaa gagaattttg gatatctaga aggaataaac ttaacttacg
     4681 ttggagatgg acgtaataat attgcgcatt cattaatggt agcaggtgct atgttaggtg
     4741 ttaatgtaag aatttgtaca cctaaatcat taaatccaaa agaggcatat gttgatattg
     4801 caaaagaaaa agcgagtcaa tatggtggtt cagtcatgat tacggataat attgcagaag
     4861 cagttgaaaa tacagatgct atatatacag atgtttgggt atcgatgggt gaagaaagtg
     4921 aatttgaaca acgtattaat ttattaaaag actatcaagt gaatcaacag atgtttgatt
     4981 taacaggtaa agattcaacg atattcttac attgtttacc agcattccat gatacaaata
     5041 cactttatgg acaagaaatt tatgaaaaat atggattagc tgaaatggaa gttacagacc
     5101 aaatctttag aagtgaacat tcaaaagtgt ttgatcaagc tgaaaataga atgcatacaa
     5161 ttaaggcagt aatggcagca acattgggga gttaatcact aaatggaacg atatgaatat
     5221 gatgtgtctg atgatataag tgtcatgtac agacacctca tattggtatt aaaggagaaa
     5281 tgaatatgaa cgaatcagga gataacaaac tcagtaaatc ttctttaatt ggactagtta
     5341 taggatccat gattggtggc ggtgcgttca atataatgtc tgatatgggc ggtaaagccg
     5401 gtggattagc cattattatt ggttggatta ttacagctat aggaatgatt tcattagcgt
     5461 tcgtatttca aaatttaacc aatgaacggc cggagctaga cggtggtatt tatagttatg
     5521 ctcaagcagg atttggcgat tttgtaggat ttatcagtgc ttggggatat tggttctcag
     5581 cgtttttagg caatgttgcc tatgcaacac tattgatgtc agcagtaggt aactttttcc
     5641 cgatttttaa aggaggcaac acattaccaa gtgttattgt cgcctcgtta ctactctggg
     5701 gtgtccattt cttgatttta aaaggcgttg aaacagcagc atttatcaat agtattgtta
     5761 ctgttgcaaa gttaataccg attttacttg taatcatatg catgataatt gcattcaatt
     5821 ttgacacttt taaaacaggc tttttcagta tgacgtcaga gggtgtattg ccatttagtt
     5881 gggcgagcac aatgagccaa gttaaaagta cgatgctagt gacagtttgg gtgtttatcg
     5941 gtatcgaagg tgcagtaatt ttttctagta gagctaaaaa taaaaaagat gtaggtagtg
     6001 ccacggttat aggacttata tcagttttaa ttatctattt cttattaact gtattagctc
     6061 aaggcgtgat tttgcaaaat catatttcgc aattagattc gccaagtatg gcacaggtgc
     6121 ttgcaactat tgtaggtggt tggggatcta cacttgtaaa tattggttta attatttcgg
     6181 tactaggtgc atggttagga tggacactgc ttgctggtga attacctttc attgttgcaa
     6241 aagatggatt atttccaaaa tggtttgcta aagaaaataa aaatggagca cctgtaaatg
     6301 cactgcttat taccaatata ttagtacaat tatttttaat aagtatgcta tttacacaga
     6361 gtgcgtatca atttgcattt tcactagcat caagtgctat tttataccct tacatgttca
     6421 gtgcatttta ccaagttaaa tacactttag agcatcgaca gcaagcaact actaaacaat
     6481 ggacgattgg tatcatagcc tcaatttatg ctatatggct tatatatgca gcaggtatca
     6541 attacttatt attgacgatg ttactttata ttccagctct tcttgtttat acaatcgttc
     6601 aaaagaataa tcagacacgt ttgattaaat cagactatat tctttttatg attattatcg
     6661 tacttgcagt tatcgggtta attaagttat tgatgggaac gataaatgtt ttttaaaagg
     6721 agcgacaaaa atatgaaaga gaaaattgtc attgcattag gcggtaatgc gatacagaca
     6781 acagaagcaa cagctgaagc acaacaaaca gctattagat gtgcgatgca aaaccttaaa
     6841 cctttatttg attcaccagc gcgtattgtc atttcacatg gtaatggtcc acaaattgga
     6901 agtttattaa tccaacaagc taaatcgaac agtgacacaa cgccggcaat gccattggat
     6961 acttgtggtg caatgtcaca gggtatgata ggctattggt tggaaactga aatcaatcgc
     7021 attttaactg aaatgaatag tgatagaact gtaggcacaa tcgttacacg tgtggaagta
     7081 gataaagatg atccacgatt tgataaccca actaaaccaa ttggtccttt ttatacgaaa
     7141 gaagaagttg aagaattaca aaaagaacag ccagactcag tctttaaaga agatgcagga
     7201 cgtggttata gaaaagtagt tgcgtcacca ctacctcaat ctatactaga acaccagtta
     7261 attcgaactt tagcagacgg taaaaatatt gtcattgcat gcggtggtgg cggtattcca
     7321 gttataaaaa aagaaaatac ctatgaaggt gttgaagcgg ttatagataa agattttgct
     7381 agtgagaaat tagcaacgct gattgaagca gataccttaa tgattcttac gaatgtagaa
     7441 aatgtattta ttaactttaa tgaacctaat caacaacaaa tcgatgatat tgatgtagca
     7501 acactgaaaa aatacgcggc acaaggtaag tttgtggaag gatcgatgtt gccaaaaata
     7561 gaagctgcga tacgatttgt tgaaagtggg gaaaacaaaa aagttatcat taccaattta
     7621 gagcaggcat acgaagcttt gattggtaat aaaggtacac acattcacat gtagcacttt
     7681 atcacgcgac aaaacattaa atatgtttct ccgttgattc aaatgaaaaa gttgtctgct
     7741 gacactttgc aaggtttgaa ggagtttaac ttatgacaga aaactttatt ttgggtagaa
     7801 ataataaatt agaacatgaa ctaaaggcat tagcagatta cattaatata ccatatagta
     7861 tattacaacc atatcaaagt gaatgttttg tcagacatta tacgaaaggc caagttattt
     7921 atttttcgcc acaagaaagt agcaatattt actttttaat tgaaggtaac attattagag
     7981 aacattacaa tcaaaatgga gatgtatatc gttattttaa taaagagcaa gtattatttc
     8041 caatcagtaa cttatttcat ccgaaagagg ttaacgaatt gtgtacagca ttaaccgatt
     8101 gtacagttct tggattgcct agagaattga tggccttttt gtgcaaagct aatgatgata
     8161 tatttttgac actttttgca ttaataaatg ataatgagca gcaacacatg aactataaca
     8221 tggcattaac aagtaaattt gctaaagatc gaattatcaa attgatatgc catctatgtc
     8281 agacagtagg atacgatcaa gatgaatttt atgaaatcaa acagttttta actattcaac
     8341 tcatgagtga tatggctggg atttcccggg aaacagctgg tcatattatt catgaactta
     8401 aagatgaaaa acttgttgtt aaagatcata aaaattggtt agtaagcaaa catttattca
     8461 atgatgtatg tgtttaatat acaatgtaaa atgaataagt tgaacatgag gtctaacgta
     8521 catttatacg ttaggccttt tttgctagca tgatgaataa tttaaaatgt tagttaaatt
     8581 tgattgttga aattacagta aaatttaagg tgatgaaaaa tttagaactt ctaagttttt
     8641 gaaaagtaaa aaatttgtaa tagtgtaaaa atagtatatt gatttttgct agttaacaga
     8701 aaattttaag ttatataaat aggaagaaaa caaattttac gtaatttttt tcgaaaagca
     8761 attgatataa ttcttatttc attatacaat ttagactaat ctagaaattg aaatggagta
     8821 atatttttga aaaaaagaat tgattatttg tcgaataagc agaataagta ttcgattaga
     8881 cgttttacag taggtaccac atcagtaata gtaggggcaa ctatactatt tgggataggc
     8941 aatcatcaag cacaagcttc agaacaatcg aacgatacaa cgcaatcttc gaaaaataat
     9001 gcaagtgcag attccgaaaa aaacaatatg atagaaacac ctcaattaaa tacaacggct
     9061 aatgatacat ctgatattag tgcaaacaca aacagtgcga atgtagatag cacaacaaaa
     9121 ccaatgtcta cacaaacgag caataccact acaacagagc cagcttcaac aaatgaaaca
     9181 cctcaaccga cggcaattaa aaatcaagca actgctgcaa aaatgcaaga tcaaactgtt
     9241 cctcaagaag caaattctca agtagataat aaaacaacga atgatgctaa tagcatagca
     9301 acaaacagtg agcttaaaaa ttctcaaaca ttagatttac cacaatcatc accacaaacg
     9361 atttccaatg cgcaaggaac tagtaaacca agtgttagaa cgagagctgt acgtagttta
     9421 gctgttgctg aaccggtagt aaatgctgct gatgctaaag gtacaaatgt aaatgataaa
     9481 gttacggcaa gtaatttcaa gttagaaaag actacatttg accctaatca aagtggtaac
     9541 acatttatgg cggcaaattt tacagtgaca gataaagtga aatcagggga ttattttaca
     9601 gcgaagttac cagatagttt aactggtaat ggagacgtgg attattctaa ttcaaataat
     9661 acgatgccaa ttgcagacat taaaagtacg aatggcgatg ttgtagctaa agcaacatat
     9721 gatatcttga ctaagacgta tacatttgtc tttacagatt atgtaaataa taaagaaaat
     9781 attaacggac aattttcatt acctttattt acagaccgag caaaggcacc taaatcagga
     9841 acatatgatg cgaatattaa tattgcggat gaaatgttta ataataaaat tacttataac
     9901 tatagttcgc caattgcagg aattgataaa ccaaatggcg cgaacatttc ttctcaaatt
     9961 attggtgtag atacagcttc aggtcaaaac acatacaagc aaacagtatt tgttaaccct
    10021 aagcaacgag ttttaggtaa tacgtgggtg tatattaaag gctaccaaga taaaatcgaa
    10081 gaaagtagcg gtaaagtaag tgctacagat acaaaactga gaatttttga agtgaatgat
    10141 atatctaaat tatcagatag ctactatgca gatccaaatg actctaacct taaagaagta
    10201 acagaccaat ttaaaaatag aatctattat gagcatccaa atgtagctag tattaaattt
    10261 ggtgatatta ctaaaacata tgtagtatta gtagaagggc attacgacaa tacaggtaag
    10321 aacttaaaaa ctcaggttat tcaagaaaat gttgatcctg taacaaatag agactacagt
    10381 attttcggtt ggaataatga gaatgttgta cgttatggtg gtggaagtgc tgatggtgat
    10441 tcagcagtaa atccgaaaga cccaactcca gggccgccgg ttgacccaga accaagtcca
    10501 gacccagaac cggaaccaag cccagacccg gatccggatt cggattcaga cagtgactca
    10561 ggctcagaca gcgactcagg ttcagatagc gactcagaat cagatagcga ttcggattca
    10621 gacagtgatt cagattcaga cagcgactca gaatcagata gcgattcaga atcagatagc
    10681 gactcagatt cagatagcga ttcagattca gatagcgatt cagattcaga tagcgattcg
    10741 gattcagaca gtgattcaga ttcagacagc gactcagaat cagatagcga ctcagaatca
    10801 gatagtgagt cagattcaga cagtgactcg gactcagaca gtgattcaga ctcagatagc
    10861 gattcagact cagatagcga ttcagactca gacagcgatt cagattcaga cagcgactca
    10921 gattcagaca gcgactcaga ctcagacagc gactcagatt cagatagcga ttcagactca
    10981 gacagcgact cagactcaga cagcgactca gactcagata gcgactcaga ttcagatagc
    11041 gattcagact cagacagcga ctcagattca gatagcgatt cggactcaga cagcgattca
    11101 gattcagaca gcgactcaga ctcggatagc gattcagatt cagatagcga ttcggattca
    11161 gacagtgatt cagattcaga cagcgactca gactcggata gcgactcaga ctcagacagc
    11221 gattcagact cagatagcga ctcagactcg gatagcgact cggattcaga tagcgactca
    11281 gactcagata gtgactccga ttcaagagtt acaccaccaa ataatgaaca gaaagcacca
    11341 tcaaatccta aaggtgaagt aaaccattct aataaggtat caaaacaaca caaaactgat
    11401 gctttaccag aaacaggaga taagagcgaa aacacaaatg caactttatt tggtgcaatg
    11461 atggcattat taggatcatt actattgttt agaaaacgca agcaagatca taaagaaaaa
    11521 gcgtaaatac ttttttaggc cgaatacatt tgtattcggt ttttttgttg aaaatgattt
    11581 taaagtgaat tgattaagcg taaaatgttg ataaagtaga attagaaagg ggtcatgacg
    11641 tatggcttat atttcattaa actatcattc accaacaatt ggtatgcatc aaaatttgac
    11701 agtcatttta ccggaagatc aaagcttctt taatagcgat acaactgtta aaccattaaa
    11761 aactttaatg ttgttacatg gattatcaag tgatgaaacg acatatatga gatatacaag
    11821 catagaaagg tatgcgaatg aacacaaatt agctgtgatt atgcccaatg tggatcatag
    11881 cgcatatgct aacatggcat atggtcatag ctattatgat tatattttgg aagtgtatga
    11941 ttatgttcat caaatatttc cactttccaa aaagcgtgat gacaatttta tagcaggtca
    12001 ctctatggga ggatatggca caattaaatt tgcattaaca caaggggata aatttgccaa
    12061 agctgtacca ttatctgctg tgtttgaagc gcaaaattta atggatctag agtggaatga
    12121 tttttcaaaa gaggccataa ttggcaatct ttcaagtgtt aaaggaactg aacatgatcc
    12181 gtattacttg ctagacaaag ctgtagctga agataaacaa attccaaaat tgctcattat
    12241 gtgtggtaaa caagactttt tatatcaaga caacttagat tttatcgatt atttatcacg
    12301 cataaatgtt ccttatcaat ttgaagatgg accaggagat catgattatg catattggga
    12361 tcaagcgatt aagcgtgcta taacatggat ggtgaatgat taattatttc ttggaaaata
    12421 tgtggctgca ttaaatacac agagtgagag atacaaacta tttacgcacg actaacattt
    12481 ctaagtgttt aaattatttt tgtattaata tgattggcgc aatttgctga tacacaaaaa
    12541 tgtttctcgt gaaacttaga tttagcttat agttttatca tcatttgtat gacttacatt
    12601 ataaatttta ttataatgag gttaacgctt tgaaaggagt catcatcatg tcgaccaata
    12661 aaaacgatta tgagcatatg ttgttttatt ttgcatataa aacctttatt actaccgctg
    12721 atgaaattat agagaagtat ggtatgagtc gtcagcatca tcgttttttg ttttttatca
    12781 ataaattacc tggtattact attaaatcat tactagaaat attagaaatt tctaaacaag
    12841 gatcacatgc aacacttcaa aaattaaaag agcaaggtct cattattgaa aaagttttag
    12901 agactgatcg acgtgtcaaa aaattatatt cgacggataa aggcgatcaa ctcattgctg
    12961 aattgaacaa ggcgcaagat gaattattgc aaaatatata tcaacaagtc ggttcggatt
    13021 ggtatgatgt gatggaagca ttggctaaag ggcgacctgg ctttgatttt attaagcatt
    13081 tgaaagatga aaaagaaagc tagcatcaga aatgttaaaa atcttcgcat tcttaaattt
    13141 aaaaaatatg tcaaaaagtg tataataaaa acatataatt taattgaact cagtttcaac
    13201 acatcttaga aaggagtttg aatgatgaaa aaattagcag ttattttaac attagttggc
    13261 ggtttatact tcgcatttaa aaaataccaa gaacgtgtta accaagcacc taacattgag
    13321 tactaaatta aaccataaaa aattcccgaa caccttgtta tagtgctcgg gaattttttt
    13381 atgctttact tgaatatatc aaatattatt tttgcgcttt ctgtattttc gatattacca
    13441 ctaaatgatt ctgatctagg tccgtaagcg taggtattaa catcctcgcc tgtatgtcca
    13501 tcggaagtcc accctgtata agatttatca tttactggct tctgaatagc gtgttgtagg
    13561 gcttttgttt gcgtttctac ttctgcggat ttttcgtctt tttctttttt aagtagtctt
    13621 tttagctttt tattctcttt tttaaccttt ttcatatcat cttgtgaaaa ttcaaatcca
    13681 taaccttcat taataacttt ttcagggtct tcacctttag ccattttttc tgtcatatat
    13741 gatccagagt gtttcataga tttaatcggt tgaggattcc attcgtatcc tttatcttta
    13801 ccaattgtta gaccaccagt agagtgatct gctgttgcaa cgacaagtgt atctttatgt
    13861 ttcttagcat attgaatggc gtcatcgaat gctttttcaa aaccttccat ttcagacata
    13921 acgcctgtaa tatcgttgga atgcgctgat ttatctatag aagcaccttc gaccattaaa
    13981 aagaatcctt ttttattgcg ctcaagctta ctaagtgcac tttgttgcat atcagctaat
    14041 gatggttcgt ctttagaagc atctattgca agtggcatat ttttatctgc aaacaaacca
    14101 agaactttat ctttatcaga ttttgataac tccttactgt tcgtggcaag gtcgtaacca
    14161 tcttttttga attttttatc taaattgcca ttacttttac cgaaatattt agcgccgccg
    14221 cctaataaaa catcaacttt atgctttccg ttgattttat ctttataaaa ttgtttagcg
    14281 atttcgtttt tatcatctct agaagtcacg tgtgcagcat atgctgctgg tgttgcatct
    14341 gttaattcag ctgttgaaac aagaccagtc gacttacctt tttcttttgc acgttcaagc
    14401 accgtcttta ctttctgctt gttactgtca acaccgatgg caccattata tgtcttatga
    14461 ccagaactaa aggctgttcc gccagctgca gaatcagtaa tattctgttt tgggtcattt
    14521 gaatatgtac gatttgtgcc ttttaaatat gaatcaaaag cagtaggggt catttcttta
    14581 gcatgcggat catttttata ataacgataa gctgtgttaa atgatggacc catgccatcg
    14641 ccaactaaaa agataacatt ttttggattt ttagtattac caaccgcgaa actttcatct
    14701 ttagaacttt tatcggattg cgcaattgca ggtgtgacag aactaaaaac cgttgacacg
    14761 ataataaggt tagcaactgc aaattttgtg gcttttttaa ctgataacat aagacatcct
    14821 cctgagtata tgactatgtc ttcagtgtaa aagaggaatt ttgagcaatt atgtagtttt
    14881 agttaaaaat atgtaaacag agtgatttag aataacaaaa aatgaatata tatgacaatt
    14941 tgttatagaa agcgttagaa tagaagcgtg tgaaaatata gaattaaata taatttgagg
    15001 tggaaaaatg atactagtaa tgttatctcc attattaatc atattcttta tagtgttgtc
    15061 tattttagaa gagcgtaaac gtacgaagaa aaagcaactc gagaaagaaa aagcaaatac
    15121 actaaatcaa aatacaaatg acacggaaag ttcaaatcaa gagccgtcat tgcagcagga
    15181 taaagaacaa aaagataaca aaggataatt caattgaagg aagaagatta tagatgaaaa
    15241 tattaattgt tgaagatgat tttgttatag cagagagttt agcatctgaa cttaaaaaat
    15301 ggaattacgg tgttattgtc gttgaacaat ttgatgatat actgtctatc tttaaccaaa
    15361 atcaacctca gcttgtattg ctagatatta atttgccaac gttaaatggt tttcattggt
    15421 gtcaagaaat ccgaaaaaca tctaatgtgc caattatatt tattagttcc cgtattgata
    15481 atatggacca aattatggca atacaaatgg ggggagatga ttttatcgaa aagccattta
    15541 acttgtcatt aacgattgcc aaaattcaag cattattgag acgaacttat gacttgtcag
    15601 tagctaatga ttcattgacg gttaaaggtt gtacattaat attagatgaa gcaaaagtcg
    15661 tgtatcaaga acaaaacata cagctatctt tgactgaatt acaaatatta aagttattat
    15721 ttcaaaatga agataaatat gtaagtagaa ctgctttaat tgaaaaatgt tgggaatcag
    15781 aaaacttcat agatgataac acattagctg ttaacatgac gcgcctgcgt aaaaaattaa
    15841 atactattgg cgttaatgat tttatcatta caaagaaaaa tgtcggatat aaagtatagg
    15901 gtgaatgcaa tgacctttct taaaagtatt actcaggaaa tagcaatagt catagttatt
    15961 tttgctttgt ttggcttaat gttttacctg tatcatttgc cattagaagc atatttacta
    16021 gcacttggcg ttattttatt attattactc atattcatag gtattaaata tttaagtttt
    16081 gtaaaaacta taagccaaca acaacaaatt gaaaacttag aaaatgcgtt gtatcagctt
    16141 aaaaatgaac aaattgaata taaaaatgat gtagagagct actttttaac atgggtacat
    16201 caaatgaaaa cacccattac tgcagcacaa ctgttacttg aaagagatga gcctaatgtt
    16261 gttaatcgtg ttcgtcaaga ggttattcaa attgataact atacaagttt agcacttagt
    16321 tatttaaagt tattaaatga aacttctgat atttctgtca ctaaaatttc gattaata
//
LOCUS       DAKTLH010000050        16254 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.050, whole genome shotgun sequence.
ACCESSION   DAKTLH010000050 DAKTLH010000000
VERSION     DAKTLH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16254
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.050"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            150..803
                     /locus_tag="O0394_001341"
     CDS             150..803
                     /locus_tag="O0394_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCY0499425.1"
                     /translation="MELKVDDFVKNIKRPYLTVLGVFVVAVSLFFDAIMFFYAKLYDK
                     LPMYLLVFMAFTAVILIMMYIQEKNENYKVEKRYVVRYLTLNVIVGYTLPLLFVSIYV
                     FGVVGFGFDVFNYCLGIILMLFISWLGLFLFYKNEFDSENPNPAVNAIAIIIKLFAFG
                     GIFYISLIVPVTQQEEIFIGLSIFINIIVDALLVRSYFNYALYKSIKKDIENEGKTM"
     gene            837..1424
                     /locus_tag="O0394_001342"
     CDS             837..1424
                     /locus_tag="O0394_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCY0499426.1"
                     /translation="MIIFILITIFAIYYIAMIASLFKSEGFSIIGLILDVVIMGTLIF
                     YYFIGARFVDHDLSNFLMFMDTGSYIFMYFAIKCLWVKPKVVNYLIAKELGESKEVIE
                     EQELDLQTSKIRGIYFFIISVVMLIITKLRMQPELQADAISMNPVFIFVGVIIILIWL
                     VLDIYRKKKYGIFLFKTIVPLVVTTWIIIATIILS"
     gene            complement(1567..2460)
                     /locus_tag="O0394_001343"
     CDS             complement(1567..2460)
                     /locus_tag="O0394_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0499427.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
     gene            2708..3001
                     /gene="esxA"
                     /locus_tag="O0394_001344"
     CDS             2708..3001
                     /gene="esxA"
                     /locus_tag="O0394_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HCY0499428.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            3085..6114
                     /gene="esaA"
                     /locus_tag="O0394_001345"
     CDS             3085..6114
                     /gene="esaA"
                     /locus_tag="O0394_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HCY0499429.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKSIADSVSGQLNQV
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLVLVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            6114..6572
                     /gene="essA"
                     /locus_tag="O0394_001346"
     CDS             6114..6572
                     /gene="essA"
                     /locus_tag="O0394_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HCY0499430.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKAVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            6544..6786
                     /gene="esaB"
                     /locus_tag="O0394_001347"
     CDS             6544..6786
                     /gene="esaB"
                     /locus_tag="O0394_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HCY0499431.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            6799..8133
                     /gene="essB"
                     /locus_tag="O0394_001348"
     CDS             6799..8133
                     /gene="essB"
                     /locus_tag="O0394_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HCY0499432.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            8155..12594
                     /gene="essC"
                     /locus_tag="O0394_001349"
     CDS             8155..12594
                     /gene="essC"
                     /locus_tag="O0394_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HCY0499433.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGIATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRIFNDEIDRRK
                     KILSQYRVTSISEYRKLTGETIPHVFILIDNFDAVKDSPFQEVFENMMIKMTREGLAL
                     DMQVTLTASRANAMKTPMYINMKTRIAMFLYDKSEVSNVVGQQKFAVKDVVGRALLSS
                     DDNVSFHIGQPFKHDETKSYNDQINDEVSAMTEFYKGETPNDIPMMPDEIKYEDYRES
                     LNLPDIVANGALPIGLDYEGVTLQKIKLTEPAMISSENPREIAHIAEIMMKEIDILNE
                     KYAICIADSSGEFKAYRHQVANFAEEREDIKAIHQLMIEDLKQREMDGPFEKDSLYII
                     NDFKTFIDCTYIPEDDVKKLITKGPELGLNILFVGIHKELIDAYDKQIDVARKMINQF
                     SIGIRISDQQFFKFRFIQREPVIKENEAYMVANQAYQKIRWFK"
     gene            12624..13016
                     /gene="esaC"
                     /locus_tag="O0394_001350"
     CDS             12624..13016
                     /gene="esaC"
                     /locus_tag="O0394_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion substrate EsaC"
                     /protein_id="HCY0499434.1"
                     /translation="MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRL
                     EFNLSFALDDIESTAKDVQTAKSSANKDSVTVKGKAPNTLYIEKRNLMKQKLEMLGED
                     IDKNKESLQKAKEIAGEKASEYFNKAMN"
     gene            13032..13346
                     /gene="esxB"
                     /locus_tag="O0394_001351"
     CDS             13032..13346
                     /gene="esxB"
                     /locus_tag="O0394_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxB"
                     /protein_id="HCY0499435.1"
                     /translation="MGGYKGIKADGGKVDQAKQLAAKTAKDIEACQKQTQQLAEYIEG
                     SDWEGQFANKVKDVLLIMAKFQEELVQPMADHQKAIDNLSQNLAKYDTLSIKQGLDRV
                     NP"
     gene            13346..14020
                     /locus_tag="O0394_001352"
     CDS             13346..14020
                     /locus_tag="O0394_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5081 family protein"
                     /protein_id="HCY0499436.1"
                     /translation="MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKAALI
                     EKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNN
                     GFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDETLENLNNESIDTIGRVLE
                     IEIYNHQGDPQQSLYNIYEQNDLLFIRYPLKDKVLNVHIGVINTFIRELFGFDTDENH
                     INKAEE"
     gene            14020..14337
                     /locus_tag="O0394_001353"
     CDS             14020..14337
                     /locus_tag="O0394_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499437.1"
                     /translation="MTLSGKISVKAETIAHVVKELESISQKYDEIAQNFGKIAQLNYY
                     SSEKAAHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYN
                     IEG"
     gene            14347..16191
                     /locus_tag="O0394_001354"
     CDS             14347..16191
                     /locus_tag="O0394_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease YeeF family protein"
                     /protein_id="HCY0499438.1"
                     /translation="MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSD
                     VSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKA
                     FKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVK
                     VIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEELEEQKKKEEAIEIAALA
                     GMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGM
                     GFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQ
                     NTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGM
                     GGIKFAETTTLRNMGQNIKRAVTPQNHVTHGPKDSMVRSEGKHSISSHEMNSSKYVES
                     PNYTKVEFGEHYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGDRN
                     SHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKFINRPFKEKGHWYNLEKE
                     WQEFLNSGKEVKNIKMEVKYSGNSQRPTIFKVEYEINGERNIRRILNK"
BASE COUNT         6128 a         2222 c         3062 g         4842 t
ORIGIN      
        1 ttggaaatct aataaactac aaaaaatagt ttgattacat aatgattctt gaaaaatgtt
       61 ggttaactta ataatatgca tttttttggc gaagaagatt tatttaactt ataaaaatat
      121 tgaagtaaga ttggggagat tatgaattta tggaattgaa agtcgatgat tttgtaaaga
      181 atataaaaag accatacttg actgtattgg gagtatttgt agttgcagtt tcattatttt
      241 ttgacgcgat tatgtttttc tacgctaaac tatatgataa attgcctatg tatttactgg
      301 tgtttatggc ctttacagct gtaattttga ttatgatgta catacaagag aaaaatgaaa
      361 attacaaagt tgaaaaaaga tatgtggtta gatatctcac acttaacgtt attgtgggat
      421 atactttgcc attgcttttt gtatctattt acgtttttgg tgtagtcggt tttggatttg
      481 atgttttcaa ttattgtcta ggtattatct tgatgttatt tatttcttgg ttaggtttat
      541 ttttatttta taaaaacgaa tttgatagtg aaaatcctaa tcctgcagtt aatgccatag
      601 caattattat aaaattattt gcttttggag gaatatttta tataagtcta atagttcctg
      661 taacacagca agaagagatt tttatagggt taagtatatt catcaatata attgttgatg
      721 ctcttcttgt taggtcatat tttaattacg cgttatataa gagtattaag aaagatatcg
      781 aaaatgaagg taagacaatg taaaggaaag acataaaaaa taataggagt ctaaccatga
      841 taatttttat tttaataaca atatttgcta tttattatat agctatgatt gctagtttgt
      901 ttaaaagtga aggtttttca ataataggtt taatattaga tgttgttata atggggacat
      961 tgatttttta ctattttata ggcgctcgct ttgttgatca tgatttaagc aactttttaa
     1021 tgtttatgga tactggatca tatattttta tgtactttgc aattaagtgt ttatgggtga
     1081 aacctaaggt agtaaattat ttgattgcaa aagaattggg tgaatctaaa gaggtcattg
     1141 aagagcagga attagattta cagacatcaa agataagagg tatctacttt ttcattattt
     1201 cagtagtgat gttaattatc actaaattaa gaatgcaacc tgagttacag gcagatgcta
     1261 tatcgatgaa tcctgtattt atttttgttg gtgttataat cattttaatt tggctagtac
     1321 tggatatata tcgcaagaaa aaatacggta tattcttatt caagacgata gtgccactag
     1381 ttgttacgac ttggattatt atagctacaa ttatactttc gtaaacgatt ttgattaatg
     1441 gggaatgtag agaacatttc tttgtaattt tataatcgaa taacatacaa aataaagcga
     1501 ccaattttgc agtgttacga attgtaaact gcgaactggt cgctttttat tgatgtcatg
     1561 attaatttaa tggatgtaat tatatgatga aacttctgaa gcagagatgg ttcttgatga
     1621 aacgatatat tcaccaatcc agttcatttc tgaaattaga atacttccat caatattaac
     1681 tttttcaacg taggctacat gaccaaatgg accatttact gtttgtaaaa ttgatcctcg
     1741 tgttgggtgt ctatctactt tgaagccatt gcttgaagct tggcctgccc agtttttagc
     1801 atctccccaa aatgtactaa tcgtgtgtcc atctttggca cgtttatcaa agacatacca
     1861 tgtacattgt ccagcagtat ataagttgtt cttacttgtg ataagaggct gatcaatgat
     1921 tttaccgtta cctaatgcta aaggtttacc gtcagcggtc tttgctttgt cattaaattc
     1981 ggcgatttgt aattcgtcat acaattcgtc taaatcgatg cctgtaataa gccctttgtt
     2041 atcttttgaa aaagcgttat ttaattcatc gtcattgtct tcgacattcg gtattgctgg
     2101 tgtcaaagga ttgcttggtg acgtttgagg cggtgtgtgt gaatcaattg cgtcattaat
     2161 gtgcgtatac tgaccactta atgaagaatg gtactgattg ttgttaagat cacgttgatt
     2221 tgcgtgttga tgattgtcgt ttgtacgtga ctggttttga tgattgttgt ttggcgtgtt
     2281 gtttttgtca tatgtataag tatacgcgcc ggtgtcttta ttcactttga actgtgcgtt
     2341 tgggtgtgct ttctttgctt cttctaatgt tttgctatca ttcgtatatg cttgagccga
     2401 gttaggcgac atactaaata aagtaagagt tgtcatcgtc agtaaaattg ttttcttcat
     2461 aataaccatt taatccttta tgtatttaat ttaattttag tatacacatt tatattacaa
     2521 aaatgaatgg ttaattaaaa atatatgggt atattcaata tatttattta aaaaaagcta
     2581 aaaatactta aaaaactata tacatatact aataatttat atattatttg agtaaggagc
     2641 actttttcaa aaaatagtgt ccctaaaaag ttttgataaa cttaaaatat tcaggaggtt
     2701 tctagttatg gcaatgatta agatgagtcc agaggaaatc agagcaaaat cgcaatctta
     2761 cgggcaaggt tcagaccaaa tccgtcaaat tttatctgat ttaacacgtg cacaaggtga
     2821 aattgcagcg aactgggaag gtcaagcttt cagccgtttc gaagagcaat tccaacaact
     2881 tagtcctaaa gtagaaaaat ttgcacaatt attagaagaa attaaacaac aattgaatag
     2941 cactgctgat gccgttcaag aacaagacca acaactttct aataatttcg gtttgcaata
     3001 agcattctga aattggcaaa gtcacatttt ctaatgtggc tttgcttatc atttttttaa
     3061 gaaaacaact gaaaggaaat aagcatgaaa aagaaaaatt ggatttatgc attaattgtc
     3121 actttaatta ttataattgc catagttagt atgatatttt ttgttcaaac aaaatatgga
     3181 gatcaatcag aaaaaggatc ccaaagtgta agtaataaaa ataataaaat acatatcgca
     3241 attgttaacg aggatcaacc aacgacatat aacggtaaaa aagttgagct gggtcaagca
     3301 tttattaaaa ggttagcaaa tgagaaaaac tataaatttg aaacagtaac aagaaacgtt
     3361 gctgagtctg gtttgaaaaa tggtggatac caagtcatga ttgttatccc agaaaacttt
     3421 tcaaaattgg caatgcaatt agacgctaaa acaccatcga aaatatcgct acagtataaa
     3481 acagctgtag gacaaaaaga agaagtagct aaaaacacag aaaaagttgt aagtaatgta
     3541 cttaacgact ttaacaaaaa cttagtcgaa atttatttaa caagcatcat tgataattta
     3601 cataatgcac aaaaaaatgt tggcgctatt atgacgcgtg aacatggtgt gaatagtaaa
     3661 ttctcgaatt acttattaaa tccaattaac gacttcccgg aattatttac agatacgctt
     3721 gtaaattcaa tttctgcaaa caaagacatt acaaaatggt tccaaacata caataaatca
     3781 ttattgagtg cgaattcaga tacgttcaga gtgaacacag attataatgt ttcgacttta
     3841 attgaaaaac aaaattcatt atttgacgag cacaatacag cgatggataa aatgttacaa
     3901 gattataaat cgcaaaaaga tagcgtggaa cttgataact atatcaatgc attaaaacag
     3961 atggacagcc aaattgatca acaatcaagt atgcaagata caggtaaaga agaatataaa
     4021 caaactgtta aagaaaactt agataaatta agagaaatca ttcaatcaca agagtcacca
     4081 ttttcaaaag gtatgattga agactatcgt aagcaattaa cagaatcact gcaagatgag
     4141 cttgcaaata acaaagactt acaagatgcg ctaaatagca ttaaaatgaa caatgctcaa
     4201 ttcgctgaaa acttagagaa acaacttcat gatgatattg tcaaagaacc tgatacagat
     4261 acaacattta tctataacat gtctaaacaa gactttatag ctgcaggttt aaatgaggat
     4321 gaagctaata aatacgaagc aattgtcaaa gaagcaaaac gttataaaaa tgaatataat
     4381 ttgaaaaaac cgttagcaga acacattaat ttaacagatt acgataacca agttgcgcaa
     4441 gacacaagta gtttgattaa tgatggtgtc aaagtgcaac gtactgaaac gattaaaagt
     4501 aatgatatta atcaattaac tgttgcaaca gatcctcatt ttaattttga aggcgacatt
     4561 aaaattaatg gtaaaaaata tgacattaag gatcaaagtg ttcaactcga tacatctaac
     4621 aaggaatata aagttgaagt caatggcgtt gctaaattga aaaaggatgc tgagaaagat
     4681 ttcttaaaag ataaaacaat gcatttacaa ttgttatttg gacaagcaaa tcgtcaagat
     4741 gaaccaaatg ataagaaagc aacgagtgtt gtggatgtaa cattgaatca taaccttgat
     4801 ggtcgcttat cgaaagatgc attaagccag caattgagtg cattatctag gtttgatgcg
     4861 cattataaaa tgtacacaga tacaaaaggc agagaagata aaccattcga caacaaacgt
     4921 ttaattgata tgatggttga ccaagttatc aatgacatgg aaagtttcaa agacgataaa
     4981 gtagctgtgt tacatcaaat tgattcaatg gaagaaaact cagacaaact gattgatgac
     5041 attttaaata acaaaaagaa tacaacaaaa aataaagaag atatttccaa gctgattgat
     5101 cagttagaaa acgttaaaaa gacttttgct gaagagccac aagaaccaaa aattgataaa
     5161 ggcaaaaatg atgaatttaa tacgatgtct tcaaatttag ataaagaaat tagtagaatt
     5221 tctgaaaaga gtacgcaatt gctatcagat acacaagaat caaaatcaat tgcagattct
     5281 gttagtggcc aattaaatca agtcgacaat aatgtgaata agctacatgc gacaggtcga
     5341 gcattaggcg taagagctaa cgatttgaat cgtcaaatgg ctaaaaacga taaagataat
     5401 gagttgttcg ctaaagaatt taaaaaagta ttacaaaatt ctaaagatgg cgacagacaa
     5461 aaccaagcat taaaagcatt tatgagtaat ccggttcaaa agaaaaactt agaaaatgtt
     5521 ttagctaata atggtaatac agacgtgatt tcaccgacat tattcgtatt attgatgtat
     5581 ttactatcaa tgattacagc atatattttc tatagttatg aacgtgccaa aggacaaatg
     5641 aatttcatta aagatgatta tagtagtaaa aaccatcttt ggaataatgt cattacgtca
     5701 ggtgttattg gtacaactgg tttggtagaa gggttaattg tcggtttaat tgcaatgaat
     5761 aagttccatg tattagctgg ctatagagcg aaattcatct taatggtgat tttaactatg
     5821 atggtcttcg tacttattaa tacgtattta ctaagacagg taaaatctat cggtatgttc
     5881 ttaatgattg ctgcattggg tctatacttt gtagctatga ataatttgaa agcagctgga
     5941 caaggtgtga ctaataaaat ttcaccattg tcttatatcg ataacatgtt cttcaattat
     6001 ttaaatgcag agcatcctat aggcttggtg ctagtaatat taacagtact tgtgattatt
     6061 ggctttgtac tgaacatgtt tataaaacac tttaagaaag agagattaat ctaatgttga
     6121 tgaatagcgt gattgcttta acttttttaa cagcatctag caataatggc ggacttaata
     6181 ttgatgtgca acaagaagag gaaaagcgaa tcaataatga tttaaatcaa tatgatacaa
     6241 cgctatttaa taaagacagc aaagcggtta atgatgcgat tgctaagcag aaaaaagaac
     6301 gacaacaaca aataaaaaat gatatgtttc aaaatcaagc gagtcactcg actcgcttga
     6361 atgaaactaa aaaagtgtta ttttccaaat ctaacttaga aaagacttcg gagagtgata
     6421 aaagccccta tattcaaaac aagcaggaga aaaaaatatt cccgtacatt ttgatgtctg
     6481 taggggcttt tttgacttta ggatttgtca ttttttcaat tcataaaggg agacgaacga
     6541 aaaatgaatc agcacgtaaa agtaacattt gattttacta attataatta cggcacatat
     6601 gacttagcag taccagcata tttaccgata aaaaacttaa tagctttagt attggatagt
     6661 ttggacattt caatatttga tgtcaataca caaattaaag tgatgacgaa aggtcaatta
     6721 cttgttgaaa atgatcgact cattgattat caaatcgctg atggagatat tttgaagtta
     6781 ctataggagg aaaaatagat ggttaaaaat cataacccta aaaatgaaat gcaagatatg
     6841 ttaacgcctt tagatgctga agaagcagct aaaacaaaat tacgcttaga tatgagagag
     6901 attcctaagt cttcaattaa accagaacat tttcatttaa tgtacttatt agaacaacat
     6961 tctccatatt ttatagatgc tgaattaact gaactacgtg acagtttcca aatacattat
     7021 gacattaatg acaatcatac accttttgat aatattaaat catttactaa aaatgaaaaa
     7081 ttacgttact tactcaatat caaaaattta gaagaagtaa atcgtacacg ctacacattt
     7141 gtgttggcac cagatgaatt atttttcaca agagatggat tacccattgc taaaacaaga
     7201 gggttacaaa atgttgttga tccattacct gtgtcagaag ctgaattttt aacaagatat
     7261 aaagcgctgg ttatctgtgc attcaatgag aaacaatcat ttgatgcttt agttgaagga
     7321 aacttagaac tacataaagg aacgccattt gaaactaaag ttattgaagc ggcaacgtta
     7381 gatttactaa cggcattttt agatgaacag tatcagaaac aagaacaaga ttatagtcaa
     7441 aattatgcat atgtacgcaa agtaggacat accgttttca aatgggttgc tatcggtatg
     7501 acaacgttaa gtgttttatt aattgcattc ttagcctttt tatatttttc agtaatgaag
     7561 cataatgagc gcattgaaaa aggataccaa gcatttgtaa aggatgatta tacgcaagta
     7621 ctaaatacgt atgatgattt agatggtaaa aaattagata aagaggcact ttacatttat
     7681 gccaaaagtt atatccaaac aaataaacaa ggtttagaaa aagataagaa agaaaattta
     7741 cttaataatg tgacaccaaa ttcaaacaaa gactacttat tatattggat ggaattagga
     7801 caaggacatc ttgatgaagc gattaatatt gccacttatt tagatgataa cgatattaca
     7861 aagttagcgt tgattaataa attaaatgag attaaaaata acggagattt atcgaatgat
     7921 aaacgttctg aagaaacgaa aaagtataac gataaattgc aagatatttt agacaaagaa
     7981 aaacaagtta aagatgaaaa agcgaaatct gaagaagaga aagcaaaagc gaaagatgag
     8041 aaattaaagc aacaagaaga gaacgaaaag aaacaaaaag aacaagcaca aaaagataaa
     8101 gaaaaacgcc aagaagctga aagaaaaaaa tagtatagga ctgaggcaaa gacaatgcat
     8161 aaattgatta taaaatataa caaacaattg aagatgctca atttgcgaga tggtaagaca
     8221 tatactatta gcgaagacga gcgtgcagat attacgttga aatcgttagg cgaagtcatt
     8281 catttagaac aaaataatca aggtacttgg caagcgaatc atacttctat taataaggtg
     8341 cttgttagaa aaggtgacct tgatgacatt acattacagc tttatacaga agctgattat
     8401 gcatcatttg cgtatccttc aattcaagat acgatgacaa ttggaccaaa tgcgtatgat
     8461 gatatggtta ttcaaagctt gatgaatgcc atcattatta aagattttca atcaatacaa
     8521 gaatcacaat acgtacgcat tgtgcacgat aaaaatacag atgtgtatat taactatgaa
     8581 ctacaagagc aactaacgaa caaagcttac attggtgatc atatttatgt tgaagggata
     8641 tggctcgaag tacaagctga tggtttaaat gtattgagtc agaatacagt ggcatcgtca
     8701 ttaattcgct taacacaaga gatgccacat gcacaggcag atgattacaa tacgtaccat
     8761 cgttcgccaa ggattattca ccgtgaaccg acggatgata ttaagattga aagaccgcca
     8821 cagccaatac agaagaacaa tacagtgata tggcgttcca ttataccgcc attagtaatg
     8881 attgctttaa ctgttgtcat ctttttagtg agaccaattg gtatttatat tttaatgatg
     8941 attggtatga gtacagtaac gatagtattt ggtattacaa cgtatttctc tgaaaagaaa
     9001 aagtataaca aagatgttga aaaacgagag aaagattaca aagcttattt ggataataaa
     9061 tctaaagaaa ttaacaaagc gattaaagca caacgtttta gtttgaatta ccattatcca
     9121 acggttgctg aaattaaaga tatcgttgaa acgaaagcac caagaatata tgaaaaaaca
     9181 tcgcatcatc acgatttctt acattataag ttaggtattg cgaatgtaga aaagtcattc
     9241 aaattagatt accaagaaga agaatttaac caacgtcgtg atgaactatt cgacgatgct
     9301 aaagaattgt atgaatttta cacagatgta gaacaagcac cattaatcaa tgatttaaat
     9361 catgggccaa ttgcatatat tggtgcacga catctcattt tagaagaatt ggagaaaatg
     9421 ctaattcaat tgtcaacatt ccatagttat catgatttag agtttctatt tgtgacacgt
     9481 gaagatgaag ttgaaacatt gaaatgggca cgttggttgc cacatatgac attgagaggg
     9541 caaaacatta gaggatttgt ttacaatcaa cgaacgcgtg accaaatttt aacgtcaatt
     9601 tatagcatga ttaaagaacg tatccaagct gtgcgtgaac gcagcagaag taatgagcaa
     9661 attattttca caccgcaatt agtgtttgtc attacagata tgtcattaat tattgatcat
     9721 gtcattttag aatatgtaaa ccaagattta tcagaatatg gtatttcatt aatctttgtt
     9781 gaagatgtga ttgaaagttt gccagagcat gtagatacca ttattgatat caagtctcgt
     9841 actgaaggcg aactgattac gaaagaaaaa gaattagttc aattgaaatt tacacctgaa
     9901 aatattgata acgtcgataa agaatatatc gcgcgacgtt tggcgaattt gatacacgtc
     9961 gaacatttga aaaatgcaat tcctgatagt attacatttt tagagatgta taacgtgaaa
    10021 gaagtagatc agcttgatgt ggttaatcga tggagacaaa acgaaacata caaaacgatg
    10081 gcagtacctt taggtgtaag aggtaaagat gatattttat cattgaactt acatgaaaaa
    10141 gcacacgggc cacatggttt agttgctggt accactggtt cagggaaatc tgagattatc
    10201 caatcataca ttttatcttt agctattaat tttcaccctc atgaagttgc attcctattg
    10261 attgactata aaggtggggg tatggcgaac ttatttaaag atttagtcca tttagttggt
    10321 acgattacaa acttagatgg cgatgaagcg atgcgtgcct taacatcaat caaagccgaa
    10381 ttgagaaaac gtcaacgttt attcggagag catgatgtta accatattaa tcaataccat
    10441 aagttattta aagaaggtat tgcgacagaa ccaatgccac atttattcat tatttccgat
    10501 gagtttgccg aattaaaatc agaacaacct gattttatga aagaacttgt atcaacggca
    10561 cgtattggac gttcgttagg tattcattta atacttgcga cacaaaaacc atcgggtgtt
    10621 gttgatgacc aaatttggtc taactctaaa tttaagttgg cattaaaagt acaagataga
    10681 caagacagta atgaaatttt aaaaacacca gatgcagcag acattacatt accaggtcgt
    10741 gcgtatttac aagttggtaa taatgaaatt tatgaattat tccaatctgc atggagtggt
    10801 gcaacatatg acatcgaagg cgataaatta gaagttgaag ataagacgat ttacatgatt
    10861 aatgactatg gtcaacttca agcaatcaac aaagacttga gtggacttga agatgaagaa
    10921 acgaaagaaa atcaaactga gttagaagcg gtcatagatc atatcgaatc tattacaaca
    10981 cgattagaaa tcgaagaagt taagcgtcca tggctaccac cattgccaga aaatgtatat
    11041 caagaagatt tagtagaaac agatttcaga aaattatggt cagatgatgc aaaagaagtg
    11101 gaattaacat taggacttaa agacgtacca gaagaacaat atcaaggacc gatggtattg
    11161 caattgaaaa aagctgggca catcgcgtta atcggaagtc caggatatgg tagaacaacg
    11221 ttcttacaca acattatttt cgatgttgca agacaccatc gtcctgatca agcacacatg
    11281 tacttgttcg atttcggtac caatggtttg atgccagtta cagacatacc acatgtcgct
    11341 gattacttta cagtagatca agaagacaag attgctaagg cgatacgtat atttaatgat
    11401 gaaattgatc gtcgtaagaa gattttaagt cagtatcgtg tcactagtat ttctgaatat
    11461 cgaaaattaa ctggtgaaac aattccgcat gtctttattc ttattgataa ctttgacgca
    11521 gtaaaagatt cacctttcca agaagttttt gaaaatatga tgattaaaat gacgcgtgaa
    11581 gggctagcat tagacatgca agtaacctta actgcttcaa gagctaacgc tatgaaaaca
    11641 ccaatgtaca ttaatatgaa aacgcgtatc gcaatgtttt tatatgataa atcagaggtg
    11701 tcgaacgtag taggacagca aaaatttgcg gttaaagatg ttgtgggtcg agcattgtta
    11761 agtagtgatg acaacgtatc attccatatt ggccaaccat ttaaacatga tgagaccaaa
    11821 tcatataatg atcaaattaa tgatgaagta tcggcgatga cagaatttta taaaggtgaa
    11881 acaccaaatg atattcctat gatgccagat gaaattaaat atgaagatta cagagaatca
    11941 ttaaacttac cagatatagt tgcaaatggt gctttaccaa ttggattaga ttatgaaggt
    12001 gttacactac aaaaaattaa attaactgaa ccagcaatga tttcatcaga aaatccgaga
    12061 gaaattgcgc atattgctga aattatgatg aaagaaattg acatattaaa tgaaaaatat
    12121 gcgatttgta tcgcagactc aagtggagag tttaaagctt ataggcatca agtggctaac
    12181 tttgccgaag aaagagaaga cattaaagcg attcatcaac taatgattga agacttaaag
    12241 caaagagaaa tggacggccc atttgaaaaa gattcacttt atattatcaa tgattttaaa
    12301 acatttattg attgcacgta tattccggaa gatgatgtta aaaagcttat tacaaaagga
    12361 ccagaacttg gcttgaacat tttatttgtc ggcattcata aagaattaat agatgcttat
    12421 gataaacaga ttgatgttgc acgtaaaatg attaaccaat ttagtatagg tattcgtatt
    12481 tcagaccaac aattctttaa atttagattt attcaacgag aacctgttat taaagaaaat
    12541 gaagcatata tggtcgcaaa ccaagcttat caaaagatta gatggtttaa atagcaatga
    12601 attaaatagg agggaggtat gttatgaatt ttaatgatat tgaaacaatg gttaagtcga
    12661 aatttaaaga tattaaaaag catgctgaag agattgcgca tgaaattgaa gttcgttctg
    12721 gatatttaag aaaagctgaa caatataagc gattagaatt taatttgagt tttgcactag
    12781 atgatattga aagcacagca aaggacgtac aaactgcaaa atctagtgct aataaggaca
    12841 gtgtaactgt taagggaaag gcgcccaata cgttatatat tgaaaaaaga aatttgatga
    12901 aacaaaagct tgaaatgttg ggtgaagata tcgataaaaa taaagaatcc ctccaaaaag
    12961 ctaaggaaat tgctggcgaa aaggcaagtg aatattttaa taaagcaatg aattaatatt
    13021 gaggtgaaga tatgggtgga tataaaggta ttaaagcaga tggtggcaag gttgatcaag
    13081 cgaaacaatt agcggcaaaa acagctaaag atattgaagc atgtcaaaag caaacgcaac
    13141 agctcgctga gtatatcgaa ggtagtgatt gggaaggaca gttcgccaat aaggtgaaag
    13201 atgtgttact cattatggca aagtttcaag aagaattagt acaaccgatg gctgaccatc
    13261 aaaaagcaat tgataactta agtcaaaatc tagcgaaata cgatacatta tcaattaagc
    13321 aagggcttga tagggtgaac ccatgatgaa agatgttaag cgaatagatt atttttctta
    13381 cgaagaatta acaattttag gtggtagtaa attgcctctc gtaaattttg aattgtttga
    13441 tccatcaaat tttgaagaag ctaaagctgc tttaattgaa aaggaattag taacagagaa
    13501 tgacaagtta actgatgcag gttttaaagt ggctacatta gtcagagagt atattagcgc
    13561 cattgtaaat attcgaatta atgatatgta ttttgcacca tttagctatg aaaaagatga
    13621 atatattttg ttaagccggt ttaaaaataa tgggtttcaa atacgaatta tcaataaaga
    13681 cattgcatgg tggtcgattg tacaatcata tcctttattg atgagacaag aaaagtccaa
    13741 tgattgggac tttaaacaaa ttgacgatga aacattggag aacttaaata atgaaagtat
    13801 cgatacgatt gggcgtgttt tagaaattga aatatacaat catcaaggtg accctcaaca
    13861 aagtttatat aacatttatg aacaaaatga tttgttattc attcgatacc cattaaaaga
    13921 taaagtgctg aatgttcata ttggtgtcat taatacattt atacgagaat tatttggatt
    13981 cgatactgat gaaaatcata ttaataaagc agaggagtaa tgacgttgag tggaaaaatt
    14041 agtgttaaag ctgaaacgat tgcacatgtt gtaaaagaat tggaaagcat aagtcaaaag
    14101 tatgatgaaa tagctcaaaa ctttggaaaa atagcgcaat taaattacta cagtagtgaa
    14161 aaagctgcac attctatgga aaatggctat agtagtgctg caacagtcat tagtggtctc
    14221 aaaggtccat tgagtacact cggtggtggc gtcatgaatt cagcacaaaa gttctttgaa
    14281 gcagatgaac attggggtac ggaatttgcc aagctttact ataatattga gggataggtg
    14341 catgacatga caaaagatat tgaatatcta acagctgatt atgacaatga aaagtcatct
    14401 atccaaagtg taatagatgc aatagagggt caagacttct tagatgtaga tacaacaatg
    14461 gatgatgcgg taagcgatgt cagttcttta gacgaagatg gcgcaatatc attaacaagt
    14521 agtgtagtag gtccacaagg atctaaatta atggggtatt atcaaaatga gttatatgat
    14581 tatgcatctc aattagattc gaaaatgaaa gaaattattg acacgccatt tatagaagat
    14641 atagataaag cattcaaagg tataacgaat gttaaattgg aaaatatact aattaaaaat
    14701 ggtggtggac atggtagaga tacctatggg gcttctggga aaattgcaaa gggagatgcc
    14761 aagaaaagtg acagcgatgt ttatagcatc gatgaaatat taaaatcgga tcaagaattt
    14821 gtaaaagtaa ttgatcagca ttacaaagaa atgaaaaaag aagataagaa attatctaaa
    14881 agtgattttg aaaaaatgat gactcagggc gcttcttgtg attacatgac agtagctgaa
    14941 gcggaagagc tagaggagca aaagaaaaaa gaagaagcta tagagattgc agcactagct
    15001 ggtatggtag ttttatcttg tattaatcct gttgctggag cagtagctat tggtgcttat
    15061 tccgcttatt cagcagcaaa tgcagccaca ggaaaaaata ttgtaactgg aagaaagcta
    15121 tctaaagaag aacgaatcat ggaaggactt tcgcttattc cattgccagg tatgggcttc
    15181 ctcaaaggtg ctgggaaaag tttaatgaaa ttaggcttca aaggcggaga aaaatttgca
    15241 gttaaaacag gattgcaaaa gacaatgcaa caagcagtta gtcgtatttc acctaaaatg
    15301 ggaatgatga aaaacagtgt gttgaatcaa tctcgtaact ttgctcaaaa tactcatgtt
    15361 ggacaaatgc tgagtaacat gcgtggtcaa gcaactcata ctgttcaaca aagtagaaat
    15421 tggattggac aacaagcaca aaatgtcaaa cgaatagtga ataatggact tgataaagaa
    15481 atagcacatc catttaaaca acaacttgca ccagcgggaa tgggtggtat aaaatttgct
    15541 gaaacaacta ctttgagaaa catgggtcaa aacataaagc gtgctgttac accacaaaat
    15601 cacgtgacac atggtccaaa agatagtatg gtgagaagtg aaggcaaaca tagtataagt
    15661 agccatgaaa tgaattcatc aaagtatgtt gaatcaccaa actacaccaa ggttgaattc
    15721 ggagaacact atgcaagact tagacctaag aaactaaagg cgaatattga atacacaaca
    15781 cctactggtc acatatatcg aaccgatcat aaaggtcgca taaaagaagt ttatgtagac
    15841 aatctctctc taaaagatgg ggatcgtaat agccatgcac aaagaactgt ggggggagag
    15901 gatagattac cagacgatga tggaggtcac ttaatcgcta gaatgtttgg tggttcaaaa
    15961 gacattgata accttgtggc acaaagtaaa tttatcaacc gtccatttaa ggagaaaggt
    16021 cattggtata atcttgagaa agagtggcaa gagttcttaa actctgggaa agaggtgaaa
    16081 aatattaaaa tggaagtaaa atatagcggt aatagtcaaa gaccgactat atttaaagtt
    16141 gaatatgaaa ttaatggtga aagaaatatt agaagaatat taaataagta gaggtgccaa
    16201 catgacattt gaagagaagc ttagcaaaat atacaatgaa attgcgaatg agat
//
LOCUS       DAKTLH010000051        16202 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.051, whole genome shotgun sequence.
ACCESSION   DAKTLH010000051 DAKTLH010000000
VERSION     DAKTLH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16202
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.051"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(637..1497)
                     /gene="folD"
                     /locus_tag="O0394_001355"
     CDS             complement(637..1497)
                     /gene="folD"
                     /locus_tag="O0394_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCY0499439.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
     gene            1698..2180
                     /gene="purE"
                     /locus_tag="O0394_001356"
     CDS             1698..2180
                     /gene="purE"
                     /locus_tag="O0394_001356"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCY0499440.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            2167..3291
                     /gene="purK"
                     /locus_tag="O0394_001357"
     CDS             2167..3291
                     /gene="purK"
                     /locus_tag="O0394_001357"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCY0499441.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            3295..3999
                     /locus_tag="O0394_001358"
     CDS             3295..3999
                     /locus_tag="O0394_001358"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCY0499442.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLK
                     QLMDAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            3999..4262
                     /gene="purS"
                     /locus_tag="O0394_001359"
     CDS             3999..4262
                     /gene="purS"
                     /locus_tag="O0394_001359"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCY0499443.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            4264..4935
                     /gene="purQ"
                     /locus_tag="O0394_001360"
     CDS             4264..4935
                     /gene="purQ"
                     /locus_tag="O0394_001360"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HCY0499444.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            4928..7117
                     /gene="purL"
                     /locus_tag="O0394_001361"
     CDS             4928..7117
                     /gene="purL"
                     /locus_tag="O0394_001361"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCY0499445.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            7096..8580
                     /locus_tag="O0394_001362"
     CDS             7096..8580
                     /locus_tag="O0394_001362"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCY0499446.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            8573..9601
                     /locus_tag="O0394_001363"
     CDS             8573..9601
                     /locus_tag="O0394_001363"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCY0499447.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDVSRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            9604..10170
                     /gene="purN"
                     /locus_tag="O0394_001364"
     CDS             9604..10170
                     /gene="purN"
                     /locus_tag="O0394_001364"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCY0499448.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRKCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            10185..11663
                     /gene="purH"
                     /locus_tag="O0394_001365"
     CDS             10185..11663
                     /gene="purH"
                     /locus_tag="O0394_001365"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCY0499449.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPATVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPSGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            11685..12932
                     /locus_tag="O0394_001366"
     CDS             11685..12932
                     /locus_tag="O0394_001366"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCY0499450.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVH
                     TEISESDHQAILDFAKRQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYKK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(13197..14003)
                     /locus_tag="O0394_001367"
     CDS             complement(13197..14003)
                     /locus_tag="O0394_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCY0499451.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDIIFIIVLIAIITMAYYCSITLPITGIDDVRLGRVG"
     gene            complement(13996..15396)
                     /locus_tag="O0394_001368"
     CDS             complement(13996..15396)
                     /locus_tag="O0394_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0499452.1"
                     /translation="MLKVSDLRLKYPSGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQLP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            complement(15411..15986)
                     /locus_tag="O0394_001369"
     CDS             complement(15411..15986)
                     /locus_tag="O0394_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCY0499453.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(16063..16163)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         5639 a         2426 c         3104 g         5033 t
ORIGIN      
        1 ttgtaaaatt aataatttag ggataatttg aaatcaaata taactttatt cgacagtttg
       61 aacttaaatt tttgtgtatc aaaacaataa gaaaagggag gcgaaaatga taaagaacca
      121 ctaatgatta actatgtagt agtactttat catagtcaca gcttctgtgt tggggcccat
      181 ccccaacttg cacattattg taagctgact tttcgtcagc ttctttgttg gggccctgac
      241 tagaattgaa aacttgttac aagcttattt tcattcaatt aactactgtc tatataaaat
      301 tgttgagcca agtacaaagt gaaatgctcc cttcaaagcc ttcatttatt caatgtctac
      361 tttgaaggga gcatttgatc gattaatgtc ccagattttt cttgttattc attgcaattg
      421 caaaataata tttaatgtgg gttaaaagac tttattaaaa ctcagttatt agggagtggg
      481 acagaaatga tattttcgta aaatttattt cgtcgtccca ccccggcaag gttgactaga
      541 attgaaaaaa gcttgttaca agcgaatttt cgttcagtca actactgcca ttataacttt
      601 gtagagcata gaacattgat ttatgtctca ggctctttac gaatcaatac ctcgacgcat
      661 tttttctgca agcaaagtat tatttaatac catagtaatt gttaatggac caacgccacc
      721 aggaactggt gtaatagctc cagcaatttc tttaaccgca tcataatcaa cgtcaccttt
      781 taatttgcca ttttcatctg gcgtattgcc aacatcgata attactgctc cttctttgac
      841 cacatctttt gttactaaac caggcttacc aactgcactg acaatgacat cagcatcttt
      901 taaatatgat gccatatctt ttgaacgaga atgtaagatt gttactgatg catttttttg
      961 aagtagtaac ttagaaactg gttgtccgac aatatgactt cgtccaatta caactgcatt
     1021 tttaccttct aaatcaatat cagcatgttt taatatttcc atgatgccga gcggtgtgca
     1081 aggtacaaaa gtttgttcat cgatatataa tttccctata tttattggat gaaaaccgtc
     1141 cacatctttt tcaggattga ttgcttctaa tattttctgt tcgctaactt gttttggtaa
     1201 tggtacttgt accaaaatac cacttacaga atcatcatta tttagtctat ttagttcgtt
     1261 taatacttct tcttcagtag ctgtttcttc caaatgtacg atttctgaaa tcataccaat
     1321 tttttcagct gctttctttt ttgatctaac ataactttga ctagcgccat cattaccaac
     1381 taatataacg gataatttag gtgtaaaacc cttttctttt agcgcttcaa cttgatcttg
     1441 taacccctgt ctgtagtctt tggcaatttg tttaccatct aaaattttag caaccataaa
     1501 aacttctcct cctattgtca atatcctaac tttgttagtt tataaaacta atttacatag
     1561 caaaagacgt aaattcaaac aaaaaatcaa aataaagttc gatttttgat tgaaaaagca
     1621 gaaattgctt gttatgctat atctataata tacaactaaa gtaattcaca attcaaactt
     1681 ttgaaagggt gtacaaagtg aaagtagcag tcattatggg cagttcttcc gattggaaaa
     1741 ttatgcaaga gagttgtaac atgttggatt attttgaaat tccgtacgaa aaacaagtag
     1801 tatccgcaca tcgtacgcca aaaatgatgg ttcaatttgc ttctgaagcg agagaaagag
     1861 gtataaacat tatcattgca ggcgctggcg gtgcggcaca tttaccaggt atggttgcat
     1921 cattaacgac gctaccagtt attggagtgc cgattgaaac aaaaagttta aagggtatag
     1981 attctttatt atcaattgtt caaatgccag gaggtattcc ggttgcaacg actgcaattg
     2041 gtgcagcagg tgctaaaaac gcaggtatac ttgcagcaag aatgttaagt attcaaaatc
     2101 cttctttagt tgaaaaacta aatcagtatg aatcttcgtt aattcaaaaa gtggaggaca
     2161 tgcaaaatga acttcaataa attaaagttt ggtgcgacta ttggcattat tggtggtggt
     2221 cagcttggaa agatgatggc acaatcagct caaaaaatgg gttataaagt ggttgtattg
     2281 gatccttctg aagattgtcc atgtagatac gttgcacacg aatttataca agccaagtat
     2341 gacgatgaaa aggcactcaa tcaattagga caaaaatgtg atgtgattac ttatgaattt
     2401 gaaaacattt cagcccaaca attaaaacta ttatgtgaaa agtacaatat tccgcaaggt
     2461 taccaagcta tacagttatt acaagatcgc ttaactgaaa aagaaacatt aaaaagtgct
     2521 ggtaccaaag ttgtcccgtt catttcagta aaagaatcta cagatattga caaagcaatt
     2581 gaaacattag gatatccttt tattgtaaaa actagatttg gtggctacga tggcaaaggt
     2641 caagttttaa ttaacaacga aaaagactta caagaaggtt ttaaattaat tgaaactagt
     2701 gaatgcgtag ctgaaaaata tttgaatatc aagaaagaag tatctcttac tgttacaaga
     2761 ggaaacaaca atcaaatcac ttttttccca ttacaagaaa atgagcatag aaatcaaata
     2821 cttttcaaaa caattgttcc agcgagaata gataaaacag ctgaggcgaa agagcaagtt
     2881 aataaaatta tccaatcgat tcatttcatt ggaacattta cagttgaatt ttttatagat
     2941 agtaacaacc aattgtatgt gaacgagata gcaccaaggc ctcacaattc cggacattat
     3001 tcaattgaag catgtgatta ttcacaattt gatactcata ttttagcagt taccggacaa
     3061 tcattaccaa attcaattga attattaaag ccagcagtca tgatgaactt actaggtaaa
     3121 gatttagatt tattggaaaa tgaatttaat gaacatccag agtggcactt acatatttat
     3181 ggtaagtctg agcgtaaaga tagcagaaaa atggggcata tgactgtact aacgaatgat
     3241 gtaaaccaaa ctgaacaaga tatgtacgct aaatttgagg ggagtaatta aagcatgaca
     3301 ttattatatg aaggaaaagc gaagcgcatt ttctcaacaa atcaagaaaa tgaattaaga
     3361 gttgaatata aagatgaagt tactgctgga aacggggcta agaaagacac aatggcaggt
     3421 aaggggcgat taaataatca aattacttct attatattta aatatttaca agaaaatgga
     3481 atagaaagtc actttattaa acaattatct gaaacagaac aattagttaa gcctgtgaaa
     3541 ataattccat tagaagtagt tgttcgtaat attgctagtg gatctattac aaagcgttta
     3601 ggttttgaaa atggtgaagt ttttagagaa ccacttgtag aatttttcta taaaaatgat
     3661 gcgttaaatg atccgttgat aacggatgac catgttaaat tgctcaatat agcatcagat
     3721 gaagatattg aaatactaaa atccaaagca ttaaagatta ataatgtgtt gaaacaatta
     3781 atggatgcta tgaatttaaa attagtagat tttaaaatcg aatttggaaa gactgagact
     3841 ggtcaaattt tgttagcgga tgaaatatct ccagatacat gtcgaatttg ggataaagct
     3901 accaatgcaa actttgataa agatgtatat agaaataaca ctggatcact gattgaaaca
     3961 tatcaaatat ttttaaacaa attggaggat ttaaaataat gaaaacaatt gaactacata
     4021 tcacattaca accacaagta ttagatacgc aaggacaaac gcttactcga gctgtacatg
     4081 acttaggtta tgcacaagtg aatgatattc gtgtaggaaa agtattatat atgacagtgg
     4141 atgaggttag tgatgaaaag gtacacaaca ttattacaac tctaagtgaa aaattgtttg
     4201 caaatacagt gattgaagaa tatagctata aagtgttaga tgatgaaaag gagaatgcat
     4261 aaaatgaaat ttgcggttct tgtttttcca ggttcgaatt gtgatagaga catgtttaat
     4321 gctgctatta aaagtggtgt tgaagcggaa tatgtagatt atagagaaac atcactaagt
     4381 ggatttgatg gcgtacttat tcctggtgga ttttcattcg gggattactt aagatctggg
     4441 gcaatggcta gtgtagcgcc gattatttcg gaagttaaac gtcttgcagc tgaaggtaag
     4501 ccagtattag gtgtttgtaa tgggtttcaa attttaactg aaataggctt attacctggt
     4561 gcattattgc ataacgattc acatttattt attagtagaa atgaagagtt agaaatagtg
     4621 aataatcaaa cggcatttac aaatctttat gaacaaggtg aaaaagttat atatcctgta
     4681 gctcacggtg aaggtcatta ttattgtact gatgaaatat atcaacaatt aaaagctaac
     4741 aatcaaatta ttctgaaata tgtgaataat ccgaacggtt catatgatga tattgcagga
     4801 attgttaacg aaaaaggcaa tgtatgtggc atgatgccac atcctgaaag agctttagaa
     4861 acgttgttag gtactgatag tggtgtgaaa ttatttgaag cgatggtaaa aagttggagg
     4921 gaacaacatg tctaaattta tcgaaccaag cgttgaagaa attaaacttg aaaaagtata
     4981 tcaagatatg ggattaagtg atcaagaata tgaaaaagtt tgcgatattt taggcagaca
     5041 acctaacttt acagaaacag gtatcttttc tgttatgtgg agtgaacatt gctcttataa
     5101 acattctaaa ccgtttttaa agcaatttcc tacgtcaggt gaccatgtgc ttatggggcc
     5161 tggtgaaggt gcaggggtag tcgatatagg tgataatcaa gccgtagtat ttaaagtaga
     5221 gtctcacaat catccatcag caattgaacc atatcaaggg gctgctacag gcgttggtgg
     5281 aatcattcgt gacattgtct ctattggggc tagacctatt aatttgttaa acagtcttag
     5341 atttggagaa ttagataata aacaaaacca aagattactt aaaggtgttg taaagggtat
     5401 cggaggttat ggtaactgca ttggtattcc aacaactgct ggtgaaatcg aatttgatga
     5461 acgttatgat ggcaatccac ttgttaatgc aatgtgtgtt ggtgttatca atcacgacat
     5521 gattcaaaaa ggcacagcaa aaggtgtagg taattcggtc atttatgttg gtttgaaaac
     5581 tggtcgagat ggtattcatg gtgctacttt tgcatctgaa gaattgacgg aagaaagcga
     5641 aagtaaacga ccttctgtac aaatcggtga tccatttgta ggtaaaaaat taatggaagc
     5701 aacacttgaa gcaattacat ttgatgaatt agttggtatt caagatatgg gtgctgctgg
     5761 tttaacatct tcatcgtctg aaatggcggc aaaaggtggt agtgggttac atttgagatt
     5821 agaacaagtg ccaacacgtg agccaggtat ttctccttat gaaatgatgc tttcagaaac
     5881 tcaagaacgt atgttactag ttgttgaaaa aggtactgaa caaaaattct tagatttatt
     5941 tgataagcac gaattggata gtgctgttat aggtgaagtt acagatacaa atcgttttgt
     6001 tttaacatat gatgacgaag tttatgctga cattccagtt gaaccactag ctgatgaagc
     6061 acctgtatat attttagaag gagaagaaaa agattataat acttctaaaa atgattatac
     6121 acacatcgat gttaaagata ctttctttaa attacttaag catccgacta tagcatctaa
     6181 acactattta tatgatcaat acgaccaaca agttggtgcc aatacgataa ttaagccagg
     6241 acttcaagca tcggtagtac gtgtggaagg cacaaataag gcaattgctt caacaattga
     6301 tggtgaagcg cgttatgtat ataacaatcc atatgaaggt ggaaagatgg tagtagctga
     6361 agcttatcga aatttaattg ccgtgggtgc aacaccatta gcaatgacag attgtttaaa
     6421 ttatggttct cctgaaaaga aagaaatcta tcaacagttg atagattcaa cgaaaggtat
     6481 ggcagaagca tgcgacattc ttaagacacc agtagtttct ggtaatgtat ctttatataa
     6541 cgaaacgaaa ggtacttcta ttttcccaac accagttgtt ggaatggtag gtttgattga
     6601 aaatgtaaat tatttaaatg attttgaacc tcaagttgga gataaattat atttaatcgg
     6661 tgatactaag gacgactttg gtggtagtca acttgaaaag ttaatttatg gcaaagttaa
     6721 tcatgaattt gagtcattag atttgagttc agaagttgaa aaaggtgaat caatcaagac
     6781 cgctattcgt gaaggactat tatcacatgt tcaaacagtt ggtaaaggtg gcttactgat
     6841 taccttagct aaactaagtg cgcattacgg tttaggatta aaatcttcaa tagatataac
     6901 aaatgcacaa ttgtttagtg agacgcaagg ccgatatgtt gtttctgtta aatcaggtaa
     6961 aactttaaat attgataatg caatagaaat tggactttta acagatagtg ataatttcaa
     7021 ggtaacaaca ccatatacag agattagtga aaatgtttca gatattaaac aaatatggga
     7081 aggggcaatt gctcaatgtt taactactca ggattaaacg aagaatgtgg cgtgtttggt
     7141 atttggaatc atcctgaagc agcgcaacta acatatatgg gacttcatag tttgcaacat
     7201 cgtggtcaag aaggtgcagg tatagttgtt tctgatcaaa atgaattaaa aggcgagcga
     7261 ggattaggct tactaactga agcgattaaa gatgatcaaa tggaacgatt aaaaggatat
     7321 caacatgcaa ttggtcacgt ccgttatgct acttcaggta ataaaggtat tgaaaatatt
     7381 caaccgtttc tgtatcactt ttatgatatg agtgtaggta tttgtcataa tggtaacctc
     7441 attaatgcta aatcattgcg tcagaattta gaaaaacaag gtgctatctt ccattcgtct
     7501 tctgatactg aagtcattat gcatttgata cgtcgaagta aagctcctac ttttgaggaa
     7561 gcgttgaaag aaagtttgcg aaaagttaaa ggcggtttta catttgcgat tttaactaaa
     7621 gatgcgttat atggcgcagt agatccaaat gctatcagac cacttgttgt aggtaaaatg
     7681 aaagatggga catacatcct tgcaagtgaa acatgtgcaa tagatgtgtt aggtgcagaa
     7741 tttgttcaag atattcatgc aggtgaatat gtcgtgatta acgataaagg tattacagtt
     7801 aaatcttata cacatcatac gacaactgca atttctgcga tggaatatat ttattttgct
     7861 agaccagact caacaatagc tggtaaaaat gtccatgcag tacgtaaagc ttctggtaaa
     7921 aaattagccc aagaaagccc tgtaaatgct gatatggtca tcggtgtacc caattcatcg
     7981 ctatcagctg cgagtggtta tgctgaagaa ataggtttgc catatgaaat gggactagtt
     8041 aaaaatcaat atgttgcaag aacatttatt caaccaactc aagaattacg tgagcaaggt
     8101 gtgagagtga agttatctgc ggtaaaagat atagtagatg ggaaaaatat cattcttgtt
     8161 gatgattcca ttgttcgcgg tacgacaatt cgacgcattg tgaaaatgtt aaaagattct
     8221 ggtgcaaata aagtgcatgt gcgtatagca tcaccggaat ttatgtttcc aagtttttac
     8281 ggaatcgatg tttcaactac ggcagaatta atttctgcaa gcaaatcacc tgaagaaatt
     8341 aaagattata ttggcgctga ttcattagca tatctatctg tagatgggtt aattgaatca
     8401 attggtttag attatgacgc gccatatagt ggcttatgtg tagaaagttt cactggagat
     8461 tatcctgcag ggttatatga ttatgaagca aattataaag cgcatttaag tcatcgacaa
     8521 aagcaatata tttctaaaaa caaacacttt tttgatagcg agggaaattt aaatgtctaa
     8581 agcatatgaa caatctggtg taaatattca tgctggttat gaagctgtag aaagaatgtc
     8641 tagtcatgtt aaacgtacga tgcgtaaaga agttatcggt ggtttaggtg gattcggtgc
     8701 tacatttgat ttatcacaat taaatatgac agcgccagtt ttagtttctg gaacagacgg
     8761 agtaggtacg aaattaaaac tagctatcga ctatggtaaa catgattcga taggtatcga
     8821 tgcagtcgca atgtgtgtta atgatatttt aacgacaggt gcagaaccat tatatttttt
     8881 agattacatt gctacaaaca aagttgttcc tgaagttatt gaacaaattg ttaaaggtat
     8941 tagtgatgca tgtgttgaaa cgaatactgc acttatcggt ggagagactg ctgaaatggg
     9001 tgaaatgtat cacgaaggcg aatatgatgt agctggattt gctgttggag cagttgaaaa
     9061 ggatgactat gtagatggtt cagaagtgaa agagggacaa gttgttatag ggcttgcgtc
     9121 aagtggcatt cattcaaatg gatacagttt agtgcgcaaa ttaattaatg aatcaggcat
     9181 tgatttggca tcaaacttcg ataatcgtcc atttatcgat gtctttttag aaccaactaa
     9241 attatatgtc aaacctgtac ttgctttgaa aaaagaagtt tctattaagg caatgaatca
     9301 tattactggt ggaggttttt atgaaaatat tccacgtgca ttgccagccg gatatgctgc
     9361 tagaattgat actacatcat ttccaacacc aaaaatattt gattggttac aacaacaagg
     9421 caatatagac acaaatgaaa tgtataacat ttttaatatg ggtattggct atacggttat
     9481 cgttgatgaa aaagatgtat cacgcgcttt gaagatttta gcagaacaaa atgtggaagc
     9541 ctatcaaatt ggtcatattg tgaaaaatga gtcaactgca attgaattgt tgggggtata
     9601 acaatggtta aaattgcgat ttttgcatca ggttcaggaa gtaactttga aaatatagtt
     9661 gagcatgttg aatcaggaaa acttgaaaat attgaagtta cggcgctata tacggatcat
     9721 caaaatgcgt tttgtataga tagagcaaaa aagcacgata ttcctgttta tattaatgaa
     9781 ccaaaacaat ttgattcaaa agcagcgtat gaacaacatt tagtaacatt attaaatgaa
     9841 gataaggtag agtggattat tctagctggc tacatgcgtc taattggtcc agacttatta
     9901 gcttcatttg aaggtaaaat attgaatata catccatctc tattgccgaa atataagggg
     9961 attgacgcaa taggccaagc atatcatagt ggcgatacta ttactggttc gacagtacat
    10021 tatgttgata gtggtatgga tacgggagaa attattgaac agagaaaatg tgatattaga
    10081 ccggacgatt caaaagaaca attagaagag aaagtaaaaa aattggaata tgagttatat
    10141 ccaagtgtta ttgctaaaat tgtaaaataa ggagtatgta tgtgatgaag aaagctattt
    10201 tgagcgtatc aaataaaaca ggtattgtag agtttgctaa agcgttaacg caattaaatt
    10261 atgaattata ttcaacaggt ggtactaaac gtatattaga tgaagcaaat gtaccagttc
    10321 gttctgtttc agacttaaca cattttccag aaataatgga tggccgtgtt aaaacattac
    10381 atccggctgt tcatggtggc attttagctg atcgaaataa accgcagcat ttaaatgaat
    10441 tatcagaaca acatatagat ttaattgata tggtagtagt taatttatat ccattccaac
    10501 aaactgttgc aaaccctgat gtgacgatgg acgaagcaat tgaaaatatt gatattggtg
    10561 gtccaacaat gttacgtgct gcagctaaaa actataaaca tgtaacaaca attgtacatc
    10621 cggcagatta tcaagaagta ttgacgcgat taagaaacga ttcgttagat gagtcatata
    10681 gacaatcatt aatgattaaa gtttttgagc atactgcaga atatgatgaa gcgattgtac
    10741 gtttctttaa aggggataaa gaaactttaa gatatggaga aaatccacaa caatcagcgt
    10801 attttgtgag aacttcgaat gctaagcaca cgattgcagg cgctaaacaa ttacatggga
    10861 aacaattaag ctataacaat attaaagatg cagatgctac actagcttta gttaaaaagt
    10921 ttgatacacc tgctacagtt gcggttaaac acatgaatcc atgtggtgtt ggtatcggtg
    10981 acacgataga acaagcattt caacatgcgt atgaagcgga tagtcaatca atatttggtg
    11041 gaattgttgc attaaaccga gctgtaacac ctgagttagc agagcaattg catagtatct
    11101 ttttggaagt cattattgca ccaaaattta cagatgaagc attagatatt ttaaaacaaa
    11161 agaagaatgt aagattatta gaaattgata tgactataga cagtaacgaa gaagagtttg
    11221 tttcagtatc tggcggatat ttagttcaag ataaagacaa ttatgtcgtg ccaaaagaag
    11281 aaatgaaagt tgttacagaa gtagcaccta cagatgaaca atgggaagca atgttattag
    11341 gatggaaagt tgtaccatca gtaaaaagta atgcaattat tttaagtaat aataaacaaa
    11401 ctgtaggtat aggtgctgga caaatgaatc gtgtcggtgc tgctaaaatt gcgttagaga
    11461 gagctattga aatcaatgat catgtagcgt tagtatctga tggatttttc cctatgggag
    11521 atacagttga acttgcagca caacatggta taaaggcaat tatccaaccg agtggttcga
    11581 ttaaagatca agattcaatt gatatggcta ataaacatgg tattgcaatg gtagtcacag
    11641 gcactcgaca ttttaaacac taattttaaa ggggcgtaac ataaatgaat gtattagtaa
    11701 ttggtgctgg tggacgagaa catgcacttg catataaact taatcaatcg aatctagtta
    11761 aacaagtgtt tgtcattcca ggtaatgagg caatgacacc tatagctgaa gtacacactg
    11821 aaatttcaga atctgatcat caagcgatac tagattttgc taaacggcaa aatgttgatt
    11881 gggtagttat aggtccagaa cagccgctaa ttgatggatt agcagacatt ttacgagcga
    11941 atggtttcaa agtgtttggt ccaaataagc aagcagctca aatcgaaggc tcaaaattat
    12001 ttgctaaaaa gataatggaa aaatataata ttccaactgc tgattataaa gaagttgagc
    12061 gaaaaaagga tgctttaaca tatattgaaa actgtgaatt gcccgttgtt gtcaagaaag
    12121 atgggttagc tgctgggaaa ggcgttatta ttgcagatac tattgaagca gccagaagtg
    12181 ctattgagat tatgtatggt gatgaagaag aaggtactgt tgtatttgaa acgtttttag
    12241 aaggtgaaga gttctcgcta atgacatttg ttaatggtga tttagcagta cctttcgact
    12301 gtattgcaca agatcataaa cgcgcatttg atcatgatga aggaccaaat actggtggta
    12361 tgggggctta ttgtccagta ccacatatta gtgacgatgt tttaaaactt acaaatgaaa
    12421 caattgcaca acccattgca aaggcaatgc ttaatgaagg ttatcaattc ttcggtgtat
    12481 tatacattgg tgctatttta actaaagatg gtccaaaagt aatagaattt aatgcccgtt
    12541 ttggtgatcc tgaagctcaa gtattattaa gtcgcatgga aagtgattta atgcagcata
    12601 ttattgattt agatgaagga aaacgtactg aattcaaatg gaaaaatgaa tctattgtag
    12661 gggtcatgtt ggcatcaaaa ggatatcctg atgcatatga aaaagggcat aaagtaagtg
    12721 gctttgattt aaatgaaaac tattttgtta gtggattaaa gaagcaaggt gatacctttg
    12781 ttacttcagg tggtagagtt atacttgcca tcggaaaagg tgacaatgta caagatgcac
    12841 agcgagacgc atacaaaaaa gtatcacaaa tacaaagtga ccatttattc tatcgtcatg
    12901 acattgcgaa taaagcacta caacttaaat aagtaaattt aaaatactaa gattagctat
    12961 gaacgaatct ataacgatag attttttcat agctttttta gttgtagagt ctaggacatt
    13021 gatttctgta ccaaatttgt gattatgcat atgtaataca aaagaggcgc cacaacatgt
    13081 ttggatgaac aaaataacat gtttgtggca cctcttttgt ttagtatgga ataaatggtt
    13141 ttctttttct atacaatgaa tttctaattt agtatctata caattatgga taaaatttaa
    13201 cctacacgac caagacgaac atcatctatg cccgtgatgg gtaaggtgat tgaacaataa
    13261 tatgccatag taataatggc aattaaaact ataataaaga ttatatcttt atatgagaaa
    13321 ggtacgttgt aatagtaagt acgaggacca tctctaaatc ctttcgactc catcgcaact
    13381 gataattgat gtgcctttct aatattttgg cttaatagag gtataattaa atgcttaaat
    13441 cgctttaacc ctctataatt tgccgcgtct atcatctgat agcgcatttt taaagatctg
    13501 cgaagctgta ttaaagaact aatcattaaa ggtatcatac gaatggcagc catgaatgca
    13561 taagcaactt ttgatttaac ctttaaatgt tgcattaaac tataaaatat catgacaacc
    13621 tgagatgtaa gtgcgattaa gataccgaaa aatgaaatag caatggttct taatgataca
    13681 tgtaaaccac gaactaaact ttctgttgta atatggataa atccgaattt caaaattgta
    13741 tggctaccat tcccgtataa aatcatgaac agggaagaga gtaatgcaaa gccaatactt
    13801 atagttataa aaattgctgt aattttaaac tgagtaccat taaacatcaa taagaaaact
    13861 aacattaaga tagtgatata aagcataaaa tcgaaattat gcacaaatat aataaagaaa
    13921 aatagtataa ttccaagaaa tagtttcgtt ataatgttga catcatcaac aaatgattgc
    13981 cgaactttcc attgctcata cattcgtatc accatcacaa tctagtaacg caccatctga
    14041 aattttaagt cttcttgatg gataacgttc aattatttca tcgtcatgtg taaccatgac
    14101 aatactttgt cccaaattaa ttcgcttttg gaaaagtttg atcaactgga atgtattatg
    14161 gctatcaagt ccaaatgtcg gttcatctaa aaagataata tcagctttag aacttagtgc
    14221 ggtagctacg ctaaggcgtc gtttttgacc aatagacaac tcataaggat gttgatcttt
    14281 tacattttgt aaatctaaaa gttttaaaag ttgtatcgtt tcatcatcac tttgatcttt
    14341 agaaaggtga ttaaaatgaa tgttaatttc atcataaacc gaatttgtta taaattgtaa
    14401 ttctgggttt tgataaacta ggtacatgtg ttttgctgca tgtttaattt ttgttaaacg
    14461 ctgattttca aaataaacat caccttgata tttaatcaat tgcataattg attcaagcaa
    14521 ggttgtttta ccactaccat ttgcccctgt aattgtaatc cactcaccta gaccaatttc
    14581 taaatctgag aatgagagca atgttgattt accgcgaata atacgtccat ttttaaattg
    14641 taataagtgt gagtttgttg ttggaaagtc aacacgactt ggtgcgaatt cccatgcacg
    14701 tggatgccac acaccatatt cactgagtaa atgaacatac ttctgtaata tgatttcagg
    14761 acattcatcg gcaatgatat ttccgttata atccatcaaa atgacgcggt cgacatgatt
    14821 ccagatgtgt ttaactttat gttcaacgat tacaaccgtt tgatcttccc aaagttcaat
    14881 tagtttagtc cataaatctt ctgttgcttg aacatctaac attgctgtcg gttcatctaa
    14941 aaacaatgtt tttgattgtt gaagaatggt ttcaacaatt gccaatttct gtttcatccc
    15001 gccacttaaa tctttgatat acgtttcagg ggtaacattt aaattgacca tatttaaagc
    15061 attgataatt aacgcatcca tgtcttcacg tggtaattgt ctattttcta aaacgaatgc
    15121 aagttcttcg tatacttttg gcatacaaaa ctggctatca gggtcttgga aaataacgcc
    15181 acttaatggg tcaacgatta gttcatcata tttcataggt aattcaatta aattaggaac
    15241 aataccactt aatacattca gaagtgtact tttaccgcaa ccagaaggac cgagtaaaag
    15301 tactttttct ttgtcttgaa tagtgatatt taaatgatcg aaaattttac gttgaccact
    15361 tggatatttt aatcgtaaat cacttacttt taacactctg aatgctcctc ttataagttg
    15421 tcgtaatctt ctttagcagc tggtctaaat aattttgtta cgcctgtctt atctaaagct
    15481 tttactaaaa ggtaagatag gacgccggcg actactgcac cactaattaa tctaaatacg
    15541 atgaataatg ttaagttcca acctgcaact tcatttaaat aaccatagaa ataatctatc
    15601 gggaaagccg cgattgctgt acaaaaacct gctaacatag ctaccataac tgaacgtgat
    15661 tgatatttaa aaattgcaaa gacaagttca cacgctaaac cttgtataaa agcgtaaacg
    15721 attgtcggaa tatcgaaacg acccataatg atagtttcgc cggcacctgc agcaaattca
    15781 gccagtaaag caatacctgg ttttggaata attagatagc agacaatcgc tgccatgaac
    15841 caaaccccgt ttgttaattg ttcgaggtga aggcctgtag cttgcacacc attgtaaaca
    15901 aaccaccata aattgtaaat aactgcgaat actactgaaa taagtacggt tactagtatt
    15961 tcagatagct ttaaaccttt tgacattttt acatcctcct aataaaaaaa cgcacaacca
    16021 tccataggaa agttatgcgt tcacaatata tattagtaaa acatatgtat agtaacactt
    16081 tcctacgcta gttcaagcta gatcaggttc aaagggtttg agggcaagcc tcatctcagt
    16141 ataaaacacc cctagtgtgt gcgatttatt taattaatta tactgtaaga cgtttgtaaa
    16201 ct
//
LOCUS       DAKTLH010000052        15837 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.052, whole genome shotgun sequence.
ACCESSION   DAKTLH010000052 DAKTLH010000000
VERSION     DAKTLH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15837
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.052"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(177..1079)
                     /gene="cvfB"
                     /locus_tag="O0394_001370"
     CDS             complement(177..1079)
                     /gene="cvfB"
                     /locus_tag="O0394_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HCY0499454.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDYLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKIALTKKGWSRMDSKE"
     gene            1226..2827
                     /locus_tag="O0394_001371"
     CDS             1226..2827
                     /locus_tag="O0394_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0499455.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNISFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSYKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            3687..4892
                     /locus_tag="O0394_001372"
     CDS             3687..4892
                     /locus_tag="O0394_001372"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCY0499456.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGMNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIISNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            4956..5945
                     /locus_tag="O0394_001373"
     CDS             4956..5945
                     /locus_tag="O0394_001373"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCY0499457.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEV
                     EFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            5947..6834
                     /locus_tag="O0394_001374"
     CDS             5947..6834
                     /locus_tag="O0394_001374"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCY0499458.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNIEALKTHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKERILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLRETYDTFKAGENE"
     gene            6831..7553
                     /locus_tag="O0394_001375"
     CDS             6831..7553
                     /locus_tag="O0394_001375"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCY0499459.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKGADVAIDFSNPNLLFPLLDEDFHLPLVVATTGEKEKLLNKLDELSQNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            7580..8299
                     /gene="dapD"
                     /locus_tag="O0394_001376"
     CDS             7580..8299
                     /gene="dapD"
                     /locus_tag="O0394_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCY0499460.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            8442..9593
                     /locus_tag="O0394_001377"
     CDS             8442..9593
                     /locus_tag="O0394_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0499461.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV"
     gene            9598..10683
                     /locus_tag="O0394_001378"
     CDS             9598..10683
                     /locus_tag="O0394_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCY0499462.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTLKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            10673..11938
                     /gene="lysA"
                     /locus_tag="O0394_001379"
     CDS             10673..11938
                     /gene="lysA"
                     /locus_tag="O0394_001379"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCY0499463.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
     gene            complement(12178..12579)
                     /gene="msa"
                     /locus_tag="O0394_001380"
     CDS             complement(12178..12579)
                     /gene="msa"
                     /locus_tag="O0394_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarA expression modulator protein Msa"
                     /protein_id="HCY0499464.1"
                     /translation="MKYLILSLVANLLVFGVLSAIGLNINILAAMMIVLVIPIMISGI
                     LFFKTNIDKTYIFFNIIFIDFYYYIYNVHLMTLPKFNNYIKAEMMELEDIDVLITSKD
                     FGFDEILFYTLYLLLILIVLYYLKKQVKHKI"
     gene            complement(12776..12976)
                     /gene="cspA"
                     /locus_tag="O0394_001381"
     CDS             complement(12776..12976)
                     /gene="cspA"
                     /locus_tag="O0394_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HCY0499465.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
     gene            complement(13147..13455)
                     /locus_tag="O0394_001382"
     CDS             complement(13147..13455)
                     /locus_tag="O0394_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCY0499466.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYSFIVLQNNEVYYKLPIID
                     "
     gene            13618..13887
                     /locus_tag="O0394_001383"
     CDS             13618..13887
                     /locus_tag="O0394_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCY0499467.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            13906..14535
                     /locus_tag="O0394_001384"
     CDS             13906..14535
                     /locus_tag="O0394_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCY0499468.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            14567..15703
                     /locus_tag="O0394_001385"
     CDS             14567..15703
                     /locus_tag="O0394_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCY0499469.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
BASE COUNT         5649 a         2485 c         2758 g         4945 t
ORIGIN      
        1 atcaaataat taatagaaat cagctttttt acattgccta agaacttaat gtcccagcct
       61 attcgtttga attatttaat tttttacttt gaaaggaatg tttaaaaaac tacttcatct
      121 gtccgattta atattgaata aaaaaaacac gcatcctacg acacgtgtaa aaatgattat
      181 tcttttgagt ccattcgact ccaacctttt ttagttaaag cgattttacc tgtttctata
      241 ttaataatct tctgtttata taagtgaccg attgcacgtt tgaatgaacc tttactcata
      301 ttgaatactt ctttaatcgc ttcagggctt gatttgtccc agaatggtaa ttcaccatca
      361 tattcaacta gtaaatcaaa gatgacttgg ccgtcatcgt ctaaacgttc atgtgcaagt
      421 ggtaaaaatg aaccatttaa ctcaccttta tcattatgcc cgataattct aacttgaaca
      481 gattcaccta atcttggttc agctttacgt tctgattcat gtacgaaaat tttgtaacct
      541 gattcgctta ataaaaagct accaattcgt aatacgcggt aaggtttggc ttcaatgact
      601 tcgtttttta aattatcgtc gtgtacaggt gtaaacatat tttctacaac agattcactc
      661 gctaaacgtc catacatatg attctcacgg tcaattcgta atgtaactag caaataatca
      721 ccaggttgtg gccatagtga tttcactttt ggtaaatctt cccatggtac taacacttca
      781 cggggtaatc caacatctat acgtgcccca tcgcgatccg ttttaagtac tttagcaaag
      841 tcatatttat ctttcgtaat atcaggcata ttttgagttg caaataattc acctgaacgg
      901 tttggataaa tgaagaaact atattcttca cctacttcta attcatcatc atcgttcatt
      961 tctgattggt ttaactttac gttttcaccg tttggtcctt ttaaaaggta agttgaacct
     1021 tgtaacccta ctacttcaag gaattctata gaacctacta tatctttgtc taatgccatg
     1081 ttattctcct tcgattcttg tattctttat tattataaca tgtgtgagtc attctatcat
     1141 tacaacttat atgaggtcga aactgtgacg ataatatgat ataattcttc cgttagaata
     1201 tcgaatgaaa aaaaggagaa aatgcatgtt acaagtaact gatgtgagtt tacgttttgg
     1261 agatcgtaaa ctatttgaag atgtaaatat taaatttaca gaaggtaatt gttatggatt
     1321 aattggtgcg aatggtgcag gtaaatcaac attcttaaaa atattatctg gtgaattaga
     1381 ttctcaaaca ggacatgttt cattagggaa aaatgaacgt ctagctgttt taaaacagga
     1441 ccactatgct tatgaagatg aacgcgtgct tgatgttgta attaaaggtc acgaacgtct
     1501 ttatgaggtt atgaaagaaa aagatgaaat ctatatgaag ccagatttca gtgatgaaga
     1561 tggtatccgt gctgctgaac ttgaaggtga atttgcagaa atgaatggtt ggaatgctga
     1621 agctgatgct gctaaccttt tatctggttt aggtatcgat ccaactttac acgataaaaa
     1681 aatggctgaa ttagaaaaca accaaaaaat taaagtatta ttagcgcaaa gtttattcgg
     1741 tgaaccagac gtactattac tggatgagcc tactaacggt ctcgatattc cagcaatcag
     1801 ttggttagaa gatttcttaa ttaactttga taatactgtt atcgtagtat cgcatgaccg
     1861 tcatttctta aataatgtat gtactcatat cgctgattta gacttcggta aaattaaagt
     1921 ttatgttggt aactatgatt tttggtatca atctagtcag ttagctcaaa agatggctca
     1981 agaacaaaac aagaaaaaag aagaaaaaat gaaagagtta caggacttta ttgctcgttt
     2041 ctcagctaac gcttctaaat ctaaacaagc aacaagtcgt aaaaaacaac tcgagaaaat
     2101 tgaattagat gatattcaac catcatcaag aagatatcct ttcgttaaat tcacacctga
     2161 gcgcgaaatc ggtaatgact tactaatcgt tcaaaatcta tctaaaacga ttgacggtga
     2221 aaaagtatta gataatattt cattcacaat gaatccaaat gataaagcaa ttttaattgg
     2281 ggatagtgaa attgcgaaaa ccacattgct taaaatatta gccggtgaaa tggaaccaga
     2341 cgaaggttca tataaatggg gtgtaacaac gtcattaagt tacttcccta aagataactc
     2401 agaattcttt gagggcgtta atatgaacct tgtagattgg ttaagacaat acgcaccaga
     2461 agatgagcaa actgaaacat ttttacgcgg cttcttaggc cgtatgctat ttagtggaga
     2521 agaagttaag aaaaaagcta gtgtactttc aggtggagaa aaagtacgtt gtatgctaag
     2581 taaaatgatg ttatcaagtg caaacgttct tttacttgat gaaccaacaa accacttaga
     2641 cttagaaagt attactgctg ttaatgatgg acttaaatca ttcaaaggtt ctattatctt
     2701 tacttcatat gactttgaat ttatcaatac aatcgcaaac cgtgttatcg atttaaataa
     2761 acaaggtggc gtttcaaaag aaattccata tgaagaatat ttacaagaaa tcggcgtttt
     2821 aaaataaaca taatatgtag aatttctttt cgaaaatctc tgtattgagg cccgaccaac
     2881 ttgcacatta ttgtaagttg gcttactgtc accttctata ttgggactta ttccgcaact
     2941 tgtattgttt gtagaaatta ggaatccaat ttctctatgt gttgcaccga caaggattga
     3001 aaaaagctag ctacaaagtc attttcattc agtcaactac tgtcaatata acattgcagg
     3061 gcctagggca ttgattcatg tccgagacta tattcaattt aatagacatt aactgggcgt
     3121 ctcgatgcta atatttatag catcgaggtg tcctttttat tttatttttt aacacatcag
     3181 catagtgaac accccttctc taaattacaa atacagttat caacgcatta aaagagtaca
     3241 tttatttaat gtgcatattg attatgctat aagtattttg aattttattt ttcaaaaagt
     3301 ctcattatca ccacctttta aaatataagc aacgctataa cttgaatttt caggttattc
     3361 caaaaaatct aaattatcgg tttacattca tatttgtcat atgtataatg aacacatacc
     3421 aatttaataa tattttgatg aggcgcatca atcatgagta aagtttagat tactgtctgc
     3481 taacagctaa atttgaaagg gtgcgatgcc gaagcaatta taatagcagt tataatttgt
     3541 tggacttttt ggttaagagc tgagagtttg tcattattta aaaataatgg agtgcatcac
     3601 ttgtatatag attaagagca agttcgcatt ccgaacttgt tcttttttta ttattgtgtg
     3661 cccttcctaa caattaggag gattatatgg taacaagaag tgtgttgaaa tttggcggat
     3721 catccgtcag tgattttaca aaaataaaaa ggatcgctga aatgttaaag gagcgagtca
     3781 atcaagatga acaattaatt gtcgttgtaa gtgctatggg taacacaaca gatcaattaa
     3841 tgacgaatgt atcaaccttg actaaagcac caaaacaaca agaactggca ttattattga
     3901 caaccggaga gcaacaaact gtatcttatt tatcaatggt attaaatgat atcggtatga
     3961 atgccaaagc aatgactggc tatcaagcgg gtattaaaac cattggccat catttaaaaa
     4021 gtaaaattgc tcaaattaat cctcaaacat ttgaacaagc ctttcaagaa aacgatattt
     4081 tagtagttgc tggatttcaa ggcatcaatg aacatcagga attaacaact ttaggcagag
     4141 gtggttctga tacgaccgct gtggcacttg ctgttagtaa tcaaatacct tgtgaaattt
     4201 ataccgacgt tgatggtgtg tatgccactg acccaagact tttaccaaag gctaaacgac
     4261 tagacatcgt ctcatatgaa gaaatgatgg aaatgagcgc tttaggtgct ggtgtacttg
     4321 aaacaagaag tgttgaatta gctaaaaact ataatatccc tttatattta ggaaaaactt
     4381 tatcgaacgt gaaaggaaca tggattatat caaatgaaga aatattagag aaaaaagcag
     4441 ttactggtgt ggctttggat aaacatatga tgcatgtaac aattagttat cccctacctg
     4501 acaatcagct acttacccaa ctatttacgg aacttgaaga aggtgctgta aatgttgata
     4561 tgatttcaca aatcgtcaac ttggatgggc tacaactatc cttcacgatt aaagatagtg
     4621 attttcatca aatttctatg attcttgaaa cattaaagaa tcaatatgaa gcattagctt
     4681 ataaaatcaa tgagcattat gtcaaaattt cattaattgg ctcaggcatg cgtgatatgt
     4741 caggtgtggc atcaaaagca tttttgacat taattgaaaa taatatacct ttctaccaaa
     4801 caacaacatc tgaaataagt atttcatacg tcattgatga ttttaatggg caacaagcgg
     4861 tagaaaaact atatgacgca tttaacattt aatggtaaaa tgattgttaa aatattctaa
     4921 aaattggaaa ttattataaa atggagtgac aagttatgac aaagttagca gttgtgggtg
     4981 caacaggatt agtaggtaca aaaatgttgg agacattaaa tcgtaaaaat attcctttcg
     5041 atgaattagt attattttca tcagcacgtt ctgcagggca agaagttgaa tttcaaggaa
     5101 aaacatatac agttcaagaa ttaactgatg ctcgtgcaag tgaacatttc gattatgtat
     5161 taatgagtgc tggtggcggt acaagcgaac actttgcccc actttttgaa aaagctggtg
     5221 caatcgttat agacaattca agtcaatggc gtatggcaga agatattgat ttaatcgttc
     5281 cggaagtcaa tgaacctaca tttacaagag gtatcattgc caatccaaac tgctctacga
     5341 ttcaatctgt tgtacctcta aaagtattgc aagatgctta tggtttaaaa cgagtggcat
     5401 atacaacata tcaagctgta tcaggttcag ggatgaaagg taagaaagat ttagctgaag
     5461 gtgtaaatgg taaagcacca gaagcatatc cacatccaat ttataataat gtgttaccgc
     5521 atattgatgt gtttttagaa aacggatata caaaagaaga acaaaaaatg attgatgaga
     5581 cgagaaaaat tttaaatgcg ccagacttaa aagtaacagc aacatgcgca cgtgtgcctg
     5641 ttcaagatag tcatagtgtt gaaattgatg taacgcttga caaagaaaca acagcagaag
     5701 atattaaagc gttatttgat caagatgacc gcgttgtttt agtagacaat ccagagaaca
     5761 atgaatatcc aatggcaatc aattctacta ataaagatga agtgtttgtt ggccgtatac
     5821 gtagagatga ttcattagaa aatactttcc atgtatggtg tacatcagac aatttattaa
     5881 aaggtgctgc attaaatgct gtacaagtat tggaacaagt tatgcgttta aaaggagcga
     5941 attaaaatga cacatttatt tgagggtgtt ggcgttgcac ttacaacccc ttttacaaat
     6001 aacaaagtta atattgaagc tttgaaaaca cacgttaatt ttttactaga aaataatgcc
     6061 caagcaatca tcgttaatgg aactactgct gagagcccta ctttaacaac agatgaaaaa
     6121 gaacgcattc taaaaacagt tattgatctt gtagataaac gtgttcctgt catagcagga
     6181 actggcacta atgatactga aaagtcaatc caagcttcaa tccaagctaa agccttaggg
     6241 gctgatgcaa ttatgttaat tacgccctac tacaacaaaa cgaaccaacg tggtttagtc
     6301 aaacactttg aagcgattgc agatgctgtg aaattaccag tcgtgctgta caatgttcct
     6361 tcaagaacga acatgacaat tgaaccagaa actgtagaaa tattaagtca acatccttat
     6421 atagttgctt taaaagatgc tacgaatgat tttgagtatt tagaagaagt gaaaaagcgc
     6481 attgatacaa attcatttgc attatatagt ggcaatgatg acaacgtcgt cgaatactat
     6541 caacgtggcg gtcaaggggt tatctctgtt attgccaatg tcattcctaa agaatttcaa
     6601 gcgttatacg atgctcaaca aagtggatta gatattcaag atcaatttaa accaatcggc
     6661 acactgttat cagctttatc agttgatatt aacccaattc ctattaaagc tctaacaagt
     6721 tatttaggat ttggaaatta tgaattacgt ctaccattgg ttagcctaga agatacagat
     6781 actaaagtgc ttcgtgaaac atatgacaca tttaaagcgg gtgaaaatga gtgaaaatat
     6841 tactaattgg ctatggcgca atgaatcaac gcgttgctag attagcagaa gaaaaaggac
     6901 atgaaatcgt tggggtcatt gaaaatacac cgaaagcaac aacgccatat caacaatatc
     6961 aacatattgc agatgttaaa ggtgccgatg ttgcaataga tttttcaaat ccaaatctgc
     7021 ttttcccttt attagatgaa gattttcatt tgccattagt tgtggcaaca actggcgaga
     7081 aagaaaaact acttaataag ttagatgaat tgagtcaaaa tatgcctgtg tttttcagcg
     7141 cgaacatgag ttatggcgtt catgcattga ctaaaatttt agcagctgct gttcccctac
     7201 ttgatgattt cgacatcgaa ttgactgagg cacatcataa taaaaaagta gatgcaccaa
     7261 gtggtacgtt agaaaaattg tatgatgtga tcgtatcttt gaaagaaaat gtaacacctg
     7321 tgtatgatag acatgaatta aatgaaaaac gccagccaca agatattggt atacattcta
     7381 ttcgtggagg tacgattgtc ggtgaacatg aagttctatt tgctggcact gatgaaacga
     7441 ttcaaatcac gcatcgtgca caatcaaaag atatttttgc gaatggtgca atacaagcag
     7501 cagaacgctt agttaataaa ccaaacggct tttatacgtt tgacaactta taaacatatt
     7561 aaaggagatc gattatttta tggtacaaca tttaacagct gaagaaatta ttcaatatat
     7621 aagtgatgct aaaaagtcta caccaataaa agtatattta aatggtaatt ttgaaggcat
     7681 cacatatcca gaaagtttta aagtatttgg ttcagaacaa tctaaagtaa tcttttgtga
     7741 agcggatgat tggaaacctt tttacgaagc atatggtagt caattcgaag atatagaaat
     7801 tgaaatggat cgtcgcaatt ctgctattcc attaaaagac ttaacaaata cgaatgcacg
     7861 aattgaacca ggtgcgttta ttagagaaca agccattatt gaagatggtg ctgtcgttat
     7921 gatgggcgca acaattaata ttggcgcagt cgttggcgaa ggtacaatga ttgatatgaa
     7981 tgctactctc ggtggtcgtg ctacaactgg taaaaatgta catgtagggg ctggcgcagt
     8041 attagcaggt gtgattgaac cccctagtgc ttcaccggtt ataatcgagg atgatgtatt
     8101 aatcggtgca aatgcagtta ttttagaagg tgtacgtgtt ggtaaaggtg ctattgttgc
     8161 agctggcgcg attgtgacac aagatgtacc agctggtgca gttgttgctg gtacacctgc
     8221 aaaagtgatt aagcaagctt ctgaagtaca agatactaaa aaagagattg tagcagcatt
     8281 aagaaaactg aatgactagt gacgtcaacg tataataatt tcaaggttga gatacgatta
     8341 tgtctcaacc ttattgttta gcattgaata aacttatgat cacaaatttc gaaatacgtt
     8401 acactattta tatagtaatt taacacaaag gattgataat tatgaatgaa ttagaatttg
     8461 ttacgaaaca tcgccgtcat ttacatcaac atcctgaatt aagcttacat gaatttgaaa
     8521 caactgctta tattaaagcg tttttagata gtttaaatat taaatacgat tgcccattgg
     8581 aaactggcgt cattgcatac ttagaaggta atggctcaca tacgatagcg tatagagctg
     8641 atattgatgc gttacctatt ttagaggaaa atgatgtgcc ttatcgcagt caatctgatc
     8701 atgtgatgca tgcttgtgga catgatggtc atacaactgc attaatgctt tttgtacaac
     8761 gttgcaaaga catgcaagat gcaggtcaat taccgcaaaa tgtcgttttc attttccaac
     8821 ctgcagaaga aactggtggc ggtgcaaatc gattaataaa agccggtgcc tttgataagt
     8881 atccaattga agcggtattt ggtattcatg ttaacccatt tgctgatgaa ggcattgcag
     8941 tgataagaga tgaagaaatt acggccagcg caacagagta tcgctttttc ttaacaggcc
     9001 tgtcaagtca tgttgctgat aaagaacaag gtcattcttg tggtgaagca ttacaacatg
     9061 tattaactca aatatcacaa attcaacaat ttcaccttaa cggtttgaaa cgaaatattg
     9121 ttcatattgg tcattttaaa gctggtgaag cgattaacac tgtaccaagt aatggctatt
     9181 tagaaggtac tattcgtaca tatgatattg atgatttaac aatcgttaaa aatcaaatgc
     9241 acaagatagc agaaagtgtc aagcttctgt ttaatgtaga ttgtgaagtt aaatttgcag
     9301 aaggttatcc ccctacaatc aatagtccga aattacgtac tcaaatagag gacgccttaa
     9361 taaaagctga tttaaatgtc tatgacaaac caacgccatt cttatttggg gaagatttta
     9421 gtttttatgg tcaacaacta gctccagctt actttgtttt tataggaaca cgaaatgaag
     9481 ataaaggttt tgtaactggt ttgcacacat cacatttaaa ttttgatgaa aaagtgttaa
     9541 taaacgtggt taatttttac gaaaatttat taaataatta caaagaggtg taatacattg
     9601 acagcaacat ggtctgtaaa taagaaaata tttttacaaa atgcaatcac agtcaaaaac
     9661 aatcagccat taatggcagt tgttaaaaat aatgcatatc actatgacct agaatttgct
     9721 gtaactcagt ttatccatgc aggtatagat acatttagca caacatcact acgagaagca
     9781 attcaaatta gacaacttgc tccagatgca acaatctttt taatgaatgc agtttacgag
     9841 tttgatttag tccgtgaaca tcaaatacat atgactttgc cgtcgttgac atattactat
     9901 aaccataaaa atgatttagc tggtattcat gttcacttag aatttgaaaa tttattacat
     9961 cggtctggat ttaaagattt aaacgaaatt aaagaagtat tgaaagatca ccatcataat
    10021 caaaatgcaa aaatgattat tagtggttta tggacccatt ttggatatgc tgatgaattc
    10081 gatgtgtcag attataatgt tgaacgttca caatggatgg aaattgttga agcactttta
    10141 tctgaaggtt atcagttcga cctaatccat gctcaaaata gtgcgagttt ttatcgggaa
    10201 ggacaaatat tactacccca ccatacacat gcgcgtgtag gtattgcgtt atacggttca
    10261 agaccatata gttcactgaa tcaacatgat atagttcagt cattaacttt aaaagcacat
    10321 gttattcaag tgcgcgaagt acaagctggt gattattgcg gttatagctt tgcctttgaa
    10381 gtgactaaaa acaatacaaa attagctgta gttgatatcg gttatggcga tggaatttta
    10441 agaactcgtg ctaaacatga agcacttatc aatggtaaac gctacccgat acgtgcatta
    10501 atgatgagcc atatgtttgt tgaagtagat ggcaatgtac atgcacaaga tgaagttatt
    10561 ctttataata atgatatccg catcgatgaa tataccttta aaggtgttgg tgcaaattct
    10621 gaacaattaa gtgctatgaa tcatgattct ttaaaaaagg agtacatttc aaatgactgt
    10681 taaatataat caaaatggcg aattaacaat ggatggtatt agtttaaaaa cgattgcaca
    10741 aagctttggt acacctacca ttgtttatga tgaactacaa attagagaac agatgcgccg
    10801 ttaccatcgc gcatttaaag atagtggatt aaaatacaat atttcatacg cctcaaaggc
    10861 atttacttgc attcaaatgg tcaaacttgt agctgaggaa gatttacagt tagatgttgt
    10921 ttctgaaggt gaattatata cagctttaga agcaggtttt gaaccgagtc gcatccattt
    10981 ccatggtaac aataaaacga aacatgaaat taggtatgct ttagaaaata atatcggtta
    11041 ttttgttata gattcattag aagaaattga attaatagac cgctatgcta atgatacggt
    11101 tcaagttgta ttacgagtta atccaggtgt tgaagcacat acacacgaat ttattcaaac
    11161 tgggcaagaa gatagtaagt ttggattatc aattcaatat ggcttagcta aaaaagcaat
    11221 tgacaaagtc caacaatcta aacacttaaa attaaaaggt gtacattgtc atattggttc
    11281 acagattgaa ggtacagaag catttattga aactgctaaa attgttttac gttggcttaa
    11341 agagcaaggc attcaagttg aattattaaa ccttggtggt ggctttggta ttaaatatgt
    11401 tgaaggtgac gaaagtttcc ctatcgaaag tggtattaaa gatattacag acgcaataaa
    11461 atccgaaatt aaagttctag gtatagatgc accagaaata ggtattgaac cgggacgatc
    11521 aattgtaggt gaagctggcg ttactttata tgaagttgga accattaaag aaattccaga
    11581 gattaataaa tatgtttcaa tcgatggcgg tatgagtgat catatcagaa ctgcacttta
    11641 tgacgcaaag tatcaagcat tgcttgttaa tagaaatgaa gaagcagatg acagtgtaac
    11701 tatagctgga aaattatgtg agtctggtga tatcattatt aaagacgcta aattaccttc
    11761 atcagtcaaa cgtggagact atcttgctat attatcaact ggtgcatatc attactctat
    11821 ggcatccaat tacaatcaaa tgcaaaagcc ttctgtgttt ttcttaaaag atggcaaagc
    11881 acgtgaagtt ataaagcgac aatcgttaag acaactcatt attaatgata caaaataaaa
    11941 ataaacaaag taatccccga agcacagaaa attatactgt gactcgggga atttttatgc
    12001 attatagatg actgtagaaa acatgcttgg acatatactc taaaaaagga agaacagcac
    12061 atttttagtt agaaagtatt tatttctttt tgtcattaat cctttatatt tagttagccc
    12121 tattcaacta atttaaagca tcatatacat accacacttt aaacaagcat catgaaatta
    12181 gattttatgc tttacttgct ttttcaaata atataaaacg ataagaatta acaacaaata
    12241 taatgtataa aacagaattt catcaaatcc aaaatcttta cttgtaatta atacatcaat
    12301 atcttctaat tccatcatct ctgctttaat ataattatta aactttggca aagtcattaa
    12361 atgtacattg taaatatagt aataaaagtc gataaatatg atgttaaaaa atatgtatgt
    12421 tttatcaata ttagttttga aaaatagtat cccagaaatc attatcggaa tcactaatac
    12481 aatcatcatt gctgctaaaa tattaatatt taatccaatt gcagataata caccgaaaac
    12541 taataaatta gcaactaaac taaggataag atatttcata aaatcacctc gtcgttgtac
    12601 accataatta tattatataa atatattttt ctattgcaaa caatgtttgt gatataaaaa
    12661 aagagttctc aatttggttg agaaactcat cgcgttaagt atttgtaata agcatataaa
    12721 aacaccttac ttcttggtaa ggtgttagtt aaaatcaatg attcaaatct aagaattata
    12781 gtttaacaac gtttgcagct tgtggaccgc ggtcgccttc aactacttca aactcaacag
    12841 cttgaccttc ttctaatgat ttgtaaccat cttggttaat tgctgaaaaa tgtacgaata
    12901 cgtcattttc tccttcaact tcgataaagc cgaatccttt ttcagcgtta aaccatttaa
    12961 ctgtaccttg tttcataatc tgaaacctcc aagactaaaa ttcattcaat atgcttattc
    13021 gcatattaca aaaaatacat gcaaatttca ctcaaatatt ctttgcaata tggaataatc
    13081 atctgcttaa cttgtattat agtgcagtta ttaccaaatt gcaatacaat aataaaaaat
    13141 attcatttaa tcaattattg gtagtttata atatacttca ttattttgta aaacaatgaa
    13201 actataaaat atttgtaaat cctcttcaca atcttcgcaa tagttcatgt cacaattatt
    13261 ataaaatgca taaatattat atttgcctgt gacatagtta tcgaactgaa ctttacattg
    13321 attaatcaat tgttgatagt gcttaaacat gtcatcaaaa tcttgatatt gatatttttc
    13381 aacaatgttt tctggccagt cactgaataa ccaccatccc tcataatcgg ctctaatttt
    13441 ggtaactgtc cacattatta aagacccctt ccatacttca aaaactctat acttattttt
    13501 atacctttat cctctgaaaa tcaagtttga cgacttgaaa caaacaaagt agtaactata
    13561 gtaattatta gtttttttaa gtaaaactac gtataataac attatgaggt ggtaaaaatg
    13621 agacatatac atttacaagt attcggacgc gttcaaggcg tcggatttag atattttaca
    13681 caacgcattg caatgaacta taacattgtc ggtactgttc aaaatgtaga tgactatgta
    13741 gagatatatg cacaagggga tgacgcagat atagagagat ttattcaagg tgtaattgaa
    13801 ggtgcctcac cagcatcaaa tgtaacaagc catcaacttg aagagttaga actaaatcaa
    13861 aaattatcgg attttcgatc aatataaaat gctaaataaa ggaagatgac agtgagatat
    13921 aatatttctc atatatttgg ggtgttagtg ggaattcctg tagcgttttt aacaagcata
    13981 tttgggatga ttgcacttga tgtatctttt ttaattgata tgtctattgg tattgttggc
    14041 tttttaatga catacctacc gatacaaaaa ctcacttcac gcaaatattt aaacgaaatt
    14101 ggtttgacta gaaaagacta tcgctatatt cgaaatcagt taaatcatac acaccaaaaa
    14161 cttagaggta ttttaaaaac gtatgtcaat ataagatcaa ttaaagattt taggcagatt
    14221 aatgatatat accaaatttc acgttctatt tatacgacag ttagacagag acctgcatca
    14281 ttttataaag ttgaaggctt tttttattct catattgata atgctttaaa tttggttgat
    14341 gcatatacac gtctagcaaa aatgcccaaa aaatcaatta atgaacagca aaagttagaa
    14401 caaacacgaa ttactttgga tgaggtcaaa cgaacattaa tcgctgattt aaagcgtctc
    14461 aacgaagatg attatgaacg tttagatatt gaaatggaat taaataagtt acatcaaaaa
    14521 catcatcaag attgatgtaa aatagcaact attggagaga tgattgatga ctgaaaataa
    14581 aagtttcaaa gaaagccatc cactagatga ttttataagt gataaagaat tatcgaatac
    14641 tactattcaa aaagaaaagt taacaattga acaacaaaaa caggtagaca caatcagtaa
    14701 acaaattaac cctttagaca atgaaggttt attagcgttt ggttctgatt tacagaaaca
    14761 aatgtctcaa ttttcacatc aaatgttgga tgaagtacaa agtaaagatg ttggtcctat
    14821 tggagatact ttgtcagatc taatgtcaaa actaaagtca gttaatccaa atgagttaaa
    14881 tactgataaa ccatctatgt taaaaagaat ttttagcaga gcaaagtcgt ctatcaatga
    14941 aatcttttca agaatgcaat cagttagtgc tcaagtcgat cgcataacga ttcaactgca
    15001 gaaacatcaa acacatttaa caagagatat tgaattatta gatacgctat atgataaaaa
    15061 caaacaatac tttgatgact tatcattgca tatcattgct gcacagcaaa aaaagttgca
    15121 attagaaaat gaaaagctac cacaattgca acagcaagcg caacaatcca ctaatcaaat
    15181 ggatattcaa caagttgcag atatgcagca atttatagat agactagata aacgcatata
    15241 tgacttacag ctttcaagac aaatagcttt gcaaactgcg ccacaaattc gtatgattca
    15301 aaatgttaat caagcacttg ccgagaagat acaaagttca attttgacaa gtattccact
    15361 atggaaaaat caaatggcca ttgcgcttac attaatgaga cagcgtaatg cagttgctgc
    15421 acaacgagct gtcactgata caactaatga tttattaaca gcaaatgctg aaatgttgaa
    15481 acaaaatgcg attgaaactg caacagaaaa tgagcgtggc attgttgatc ttgatacatt
    15541 gaaacgtaca cagcgtaaca ttattgagac aattgaagaa acattaatta ttcaacaaca
    15601 cggtcgcgaa gaacgacaat tagctgaaaa agaattacaa caattagaac aagatttaaa
    15661 gtcacattta gtgaacatca aaggaccgaa taaacaatca taaattacga attagatata
    15721 acaaaaaaga gcttgggaca ttaagttcct taaagttctt aggcaatgta aaaaagctga
    15781 tttctattat ttatttgata gaaatcagct tttttgatat gtattttata atgtaca
//
LOCUS       DAKTLH010000053        15802 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.053, whole genome shotgun sequence.
ACCESSION   DAKTLH010000053 DAKTLH010000000
VERSION     DAKTLH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15802
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.053"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(85..1212)
                     /locus_tag="O0394_001386"
     CDS             complement(85..1212)
                     /locus_tag="O0394_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCY0499470.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSNIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(1574..1951)
                     /locus_tag="O0394_001387"
     CDS             complement(1574..1951)
                     /locus_tag="O0394_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCY0499471.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
     gene            complement(2367..2960)
                     /locus_tag="O0394_001388"
     CDS             complement(2367..2960)
                     /locus_tag="O0394_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCY0499472.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            3024..3407
                     /locus_tag="O0394_001389"
     CDS             3024..3407
                     /locus_tag="O0394_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HCY0499473.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHDRKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            3538..5943
                     /gene="mnhA1"
                     /locus_tag="O0394_001390"
     CDS             3538..5943
                     /gene="mnhA1"
                     /locus_tag="O0394_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HCY0499474.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            5936..6364
                     /gene="mnhB1"
                     /locus_tag="O0394_001391"
     CDS             5936..6364
                     /gene="mnhB1"
                     /locus_tag="O0394_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HCY0499475.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            6364..6705
                     /gene="mnhC1"
                     /locus_tag="O0394_001392"
     CDS             6364..6705
                     /gene="mnhC1"
                     /locus_tag="O0394_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HCY0499476.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            6698..8194
                     /gene="mnhD1"
                     /locus_tag="O0394_001393"
     CDS             6698..8194
                     /gene="mnhD1"
                     /locus_tag="O0394_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HCY0499477.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVYFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGSIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            8196..8675
                     /gene="mnhE1"
                     /locus_tag="O0394_001394"
     CDS             8196..8675
                     /gene="mnhE1"
                     /locus_tag="O0394_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HCY0499478.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            8675..8968
                     /gene="mnhF1"
                     /locus_tag="O0394_001395"
     CDS             8675..8968
                     /gene="mnhF1"
                     /locus_tag="O0394_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HCY0499479.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            8946..9302
                     /gene="mnhG1"
                     /locus_tag="O0394_001396"
     CDS             8946..9302
                     /gene="mnhG1"
                     /locus_tag="O0394_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HCY0499480.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            9551..10705
                     /locus_tag="O0394_001397"
     CDS             9551..10705
                     /locus_tag="O0394_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCY0499481.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     YTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKELDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISGGVINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(10761..11135)
                     /locus_tag="O0394_001398"
     CDS             complement(10761..11135)
                     /locus_tag="O0394_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HCY0499482.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHRGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(11154..12470)
                     /locus_tag="O0394_001399"
     CDS             complement(11154..12470)
                     /locus_tag="O0394_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HCY0499483.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(12881..14356)
                     /locus_tag="O0394_001400"
     CDS             complement(12881..14356)
                     /locus_tag="O0394_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HCY0499484.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVFYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSEVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(14487..15695)
                     /locus_tag="O0394_001401"
     CDS             complement(14487..15695)
                     /locus_tag="O0394_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0499485.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
BASE COUNT         4987 a         2798 c         2415 g         5602 t
ORIGIN      
        1 ttactaagaa tatttttatt tataaatatt gaaaaccact aacaaattgc atacacaata
       61 tcattagtgg taacagttaa acacttattt atctttacgt ggtaatggat aaaacccttc
      121 attaaattga tgccataaat taggagttaa gtgatgatta tcttctctct ctggatcaaa
      181 gtaattaata atttcatctt cccgtccatc tttaattttt tcaacaaatt gataatccag
      241 tagtagctct ctaccaatgg ctactaagtc aacaccaaca ttttcaactg catctaaagc
      301 ttcgtcagct gtgaaaattg aaccaatacc gataagtggc atacgaccat ttatccattt
      361 gtgaattaaa ggcagtcttt cttgtccagc gtatttacct tcacgtgtcg ttgcatgcgt
      421 atccatcatt gaaacatgaa tatagtcgat tggcatatgg ctaattttat taacgagttc
      481 ctctgtaatt tccattgtga ttcctggaga ctccgcttcc tctggagata atctgtatcc
      541 aactataaag tctttattgc catacgcttc tttcgcttta agtacttctt caatcacagc
      601 gaccgggaat ttatattgat ttgcccatac atcatttctt ctattatagt atggtgatac
      661 aaattgatga attaagtaat gattcgcgcc atgtatttca acaccatcaa accctgcttc
      721 aattgcacgt cgcgttgctt caccaaaatc cttgattgct tgttcaatct cttcattcgt
      781 catttctcta gcactatgtt cttgtttctg accaaaactt tttaaagaaa ttggacttgg
      841 tgctacgacg tctccatcag gtgttaattc aggcaatgct tgtgcaccgc catgatgtat
      901 ttgtacgagt gctttggcac cgtttttctt cattgctgta gctaatcgtt ttagtccttc
      961 aatattactg tcatgcgcga ttgatggctg tcctggaaat gcttttccga catcactcac
     1021 attactcgca gcattaattg taataccaac atcttgtgaa cgcttttcaa tataaggaag
     1081 ttctacatct gaaatagtac catcatcatt tgaagaaata tgtgttaaag gggctaacac
     1141 aaatcgattt ctcaactcta ctccatttgg taattctact ttatcaaaca atggttcgta
     1201 tttacttttc attacttaat tcctccttaa aattattgag ttgactataa catgtaattt
     1261 tttcattctc caattatctg tttatatagt tgcaattcgt ttaatacttt aagttgtagt
     1321 tctcatttct aaaataaata aaaaaaacaa atccacatac aatattgcaa atcaacgatt
     1381 ctatctaaca aagataatgg atatgtcacc ttaaaccatt tgtacgtgta aatagaatca
     1441 atcatattac gttatcgtag acaaaaagag attagtatct aataaaattt aatagatact
     1501 aatctctttt atagtctaag aaactttcat ttcagaccct ttgtatgatt ttcggtacga
     1561 tttatctgtt cctttagtcg tttcgaattg ctcgctttga ctgttttatc cagattggta
     1621 agcgttcttt aagtgtttga aacccatatt tttctgtttc tctgatttca tctaatactg
     1681 attgtttttc cttcttacgc tcataatttt tgaagtaatc attatccttt aatgatagat
     1741 taagctttcc ttcatcatct atagacaaaa ttttagcttt aacaatttgg ccttctgata
     1801 gaaatttctt caaattatga acgtagtcat ccataatttc tgatatatga atcagtcctt
     1861 cagtatgatt aggggtctca acaaacgcac cgtatggttg aataccagtt acacgcacct
     1921 tgatatgttg gccaattttg tagttattca acttaataac cccttatttc gtttacttaa
     1981 cactattatc atagcacaaa tcgaaatggt ggactaatta aaacatcaaa tcatacgaat
     2041 attattttat aaatgaataa gaattctaca aacttggatt tacaaataaa attagcaaca
     2101 aattcctatc taaatcaata tgatatccag ctactttgtt ttcaatttat ttactatttg
     2161 aatagcaatt ctctgtaaaa acataaaaag caacctcaac tattatcttt aaaatacaca
     2221 tctatttaaa aactgtgctt tcttgataaa tatattgaga ttgctcatca tattagttta
     2281 tttattttcg ttggaatata tactaatcca ttcataaatt agctttatcc gagtttaaaa
     2341 tgttggtagt taattatgtt tagatattat tcttcaacat caatagattc aattacaaca
     2401 tcatgaagtg gtttatcttg tggtcccact tttgtatttg caatatcttc taatgtagtt
     2461 tcaccatcaa tgatttgacc gaatactgta tgtttttgat ctaaccatgg tgtaccaccc
     2521 ttttcgccat atgcatcaac gattggttga ggccagccac catctgcaag ttgacttaac
     2581 atattttgag gtacttcttt catttgaaca atgaaaaatt gtgaaccatt agtattaggt
     2641 cctgagttag ccattgataa tgcgccatat aagttaaatg catttaatga aaattcatct
     2701 tcaaaagcac cgccataaat actttcgcca cccataccag tagctgttgg atcgccacct
     2761 tgaatcatga agtcattaat gacacggtgg aatgtgattc catcataata accatttttt
     2821 gcatgtgtca caaaattttc aactgttttt ggtgcaatat ttggaaataa tttgaatgtc
     2881 atgtcacctt tatttgtgtg cataaccact ttgatttcac cttgttgtac ttctttgttt
     2941 aactgtggat agttagccat ttatattctc cattcatgtt atgatacata tatattaaca
     3001 tacttttaag ggaggaaaat agcatgaagc tatatcgatt ttcacacaaa acaggtagct
     3061 atggtgtcac gattgttgaa gaaaatggag acgatgtcct tgttaaagtt gaacaagtta
     3121 tcaaacaccc gaaacaaggt gatttacata accctactga aactgaaggt gtatttttcc
     3181 atgaccgcaa agcattaagc cattttgaaa aacgctatac gaaacgttca caattacgcg
     3241 acttcaatgt tgatgtattg cgctatgaag attcacttca acaagcaatt acagatttag
     3301 aaactaaact taaagctcag caaacaaaac atgctgaaat gtctttagct agtttagcac
     3361 gcttaaaaga agattattca cttcaataca aagaaaattt taaataaaaa atgacgtgtt
     3421 tcttcttatt taaatacttc tgaaaataaa gcacaacaag gagtgtgtac gtttttcaga
     3481 actatatcat tcatgtataa tgaataacat aaatattttt taaaggagga caacatagtg
     3541 agtttgttac atattgcggt gatattaccg ttaatattcg cactcattat tcctatcctg
     3601 tatagatttt ttaaacgtat acatttagga tggttcgtat tacctgtccc tatcgtcata
     3661 tttatttata tgcttacatt aattaaaacg acgatgtcag gtaatactgt aatgaaaacc
     3721 ttaaattgga tgcctcattt tggtatgaac tttgatttat atcttgatgg cttaggttta
     3781 ctctttagtt tgcttatttc aggaatcggc agtttagtag tattatattc aatcggctac
     3841 ttaagtaagt cagaacaact agggaatttt tattgttatt tattactatt tatgggtgca
     3901 atgttaggtg tcgtactttc agataatgtt attattctat atttattctg ggagctaacg
     3961 tcattttcta gttttttact tatttcattc tggcgcgaac gacaagcatc tatatatggt
     4021 gctcaaaaat cgctcataat cacagtattt ggtggtctat cattattagg tggtatcatc
     4081 ttactcgcta taccaacaca atcatttagc attcagtaca tgattcaaca tgcttctgaa
     4141 attcaaaata gcccattctt catttttgcg atgattttaa tcatgattgg tgcttttaca
     4201 aaatctgctc agtttccatt ttatatttgg ctaccagatg caatggaagc acctacacca
     4261 gtcagtgctt atcttcattc agcaacaatg gttaaagcag ggttatattt aatcgctcga
     4321 atgacaccaa tcttcgcagc atcgcaaggt tgggtatgga ctgtcacact cgttggttta
     4381 attacattat tctgggcttc gctaaacgca acaaaacaac aagacttgaa aggtatactg
     4441 gcattttcaa cagtatccca attaggtatg attatggcga tgcttggaat cggtgccatt
     4501 agttatcact atcaaggcga tgatagcaaa atatatgctg ctgcatttac ggctgcaata
     4561 ttccatttaa tcaaccatgc aacatttaaa ggtgccctat ttatgattac aggtgcagtg
     4621 gatcattcta ctggtacacg tgatgttaag aaattaggtg gcttactaac aattatgcct
     4681 atttcattta caatcactgt tattacagca ttaagtatgg ctggtgtacc accgttcaat
     4741 ggtttcttat caaaagaatc atttttagaa acaacattta cagcaagcca agcaaattta
     4801 ttcagtgttg atacattagg ctacctattc ccaatcattg gtatagtggg cagtgtcttc
     4861 acatttgtat attcaattaa atttataatg catatatttt tcggacaata taaacctgaa
     4921 caattaccta aaaaagcaca cgaagtatca atattaatgc ttttatcacc agctatttta
     4981 gctacattag taattgtatt tggtttattc cctggcattt taactaattc gattattgaa
     5041 ccagctacat cttcgattaa tcatacggta attgatgatg ttgaattcca tatgttccat
     5101 ggattaacgc ctgccttttt atcaacatta gtgatttata ttttaggtat cttactaatt
     5161 gtgacattta gttattgggt gaaattgttg caacgacaac ctggtaaact aacattcaat
     5221 tattggtaca acagaagtgc aaatgttatt ccaaactact ctgaaaagat gacgaatagc
     5281 tatgttaccg attattcacg taataattta gttatcattt ttggtgcatt aattttatta
     5341 acatttgtca ctatattctc agtacctttc aacattaatt ttaaagacgt tagtccgatt
     5401 agaattttcg aagtttgtat cgtaatttta ttattaagtg ctgccttttt aattcttttc
     5461 gctaaatcaa gattatttag catcattatg ttaagtgcag ttggatatgc tgtgtctgta
     5521 ttgtttatat tctttaaagc acccgactta gcattgacac agtttgtcgt tgaatctatt
     5581 tcaacagcat tgttcttatt atgcttctac cacttaccaa atttaaatcg ttataatgaa
     5641 aagcgctcat tccagcttac taatgcttta attgctggtg gtgttggttt gtccgtcatt
     5701 attatcggtt taattgcata cggaaataga cattttgaat cgatatctaa gttttatcaa
     5761 gagcacgttt atgatttagc tcacggtaaa aacatggtaa acgtcatact tgttgatttc
     5821 cgtggtatgg atactttatt cgagtcatct gtgcttggta tcgcaggatt agctgtatat
     5881 acaatgatta aattacgtaa aaagcgtcaa acgcaaggta acgaggtgaa aaatcatgaa
     5941 tagacaacaa aatgatctaa ttttgcaatt tgctgcagtt attatctttt tcatggtaat
     6001 ggtctttggt ttttcacttt tcttagcagg acattataca cctggtggcg gtttcgtcgg
     6061 cggattatta ttcgcaagtt cacttgttat cattacgatt gcatttgata tagaaacaat
     6121 gcgtaaaatt ttcccattag attttaaaat cttgataggt atcggattag ttttttgtat
     6181 tgcgacgcct atcgcaagtt ggtttttagg caaaaatttc tttacgcatg tcacatttga
     6241 tataccgtta tttatattag aacctgttca tatgacaaca gcagtattct ttgatttcgg
     6301 tgttttatgt gcagtagttg gtactgtcat gacaattatt atttcgattg gagagaacga
     6361 atagtggaaa ttattatgat ttttgttagt ggtattctca cagcaattag tgtctatctc
     6421 gttttgtcta aaagtctgat acgaattgtt atgggaacta cactattaac acatgcagca
     6481 aatttatttt taataactat gggcggactt aaacatggta ctgttccaat ttatgaagcg
     6541 aacgtaaaaa gctatgttga tcctatcccg caagcactta ttttaacagc aatcgttatc
     6601 gcctttgcga caacagcctt tttcttagta ttagcattta gaacatataa agaattaggc
     6661 acagataacg ttgagagtat gaaaggagtt ccagaagatg attgaaagta atatgcttgt
     6721 gttaacgcta gttattccag taattacagc catattactt gtctttattg gaaagcgtcc
     6781 tattataaag cgttatgtcg cactaggcgg tacattatta accttggtcg cggcaattat
     6841 caatttagca aatgttgtta aacatggacc aatacgcgta gagctaggtt catggaaagc
     6901 accatacagt attgtctttg tattagacat attcagtgcc ctactaataa ttactagtat
     6961 tattattaca gctattgtga ttttatattc ataccaaaca attggtattg aacgtgaaag
     7021 atattattat tatttttcag tactatttat gttaataggt atcattggtg cattcacaac
     7081 tggtgacatt ttcaacctat tcgtatattt cgaagtattt ttaatgtcat cttatttctt
     7141 attagtaatt ggttctacaa aaattcaact acaagaaaca ataaaatatg ttcttgttaa
     7201 cgttgtatcg tcatccttct tcgtaatggg tgtagcgatt ttatattcag tagtaggaac
     7261 attgaatcta gcagatataa gcaataaatt agctaacctt tcagcgcatg acagtggctt
     7321 agtcaatatc gttttcatac tatttatctt tgtttttgct acaaaagctg gcgtattccc
     7381 aatgtttgtt tggttaccta gtgcatacta tgcaccacca attccaatca ttgcattctt
     7441 tggtgctttg ttaacaaaag ttggtgttta tgcaatcgct agaacattga gcctattctt
     7501 tagtgataat gtaagctttt cacattatgt cattttattc ttagcactat taacgatcat
     7561 ctttggttgt gtcggtgctg ttgcatatgc caacattaaa aaaatcatcc tatacaatgt
     7621 catgattgct gtaggtgtga tcttagtcgg cgttgcaatg atgacagagt caggtatgat
     7681 tgggtctatt tactatacat tgcacgacat gttagtaaaa cttgcattat tcttgcttat
     7741 cggaattatg attaaaatta caggcactgc tgatttaaga caatttggtg gcttaattaa
     7801 acgatatcct gtacttggtt ggtcattctt tatcgcagca ttaagtttag ctggcattcc
     7861 cccactcagt ggcttttatg gaaaattttt tattgtccaa tcaacatttg aaagaggatt
     7921 ttatttaagt ggtgtcatcg tcttattatc aagtttagtc gtattatatt ctgtcattcg
     7981 catattccta caaggctttt tcggtcaacc aaaaggatat gatcttaata acaaagtcga
     8041 cgtcaaatat ttaactacga ttgcaatcgt tgcagttgtg ataactgttt tatatggatt
     8101 gtcagcagat tacttatatc caatggtaaa agctggtgca gaaacattct acaatccatc
     8161 aacctacgtt aaagctgtat taggaggtaa ataacatggc tgtacaatta gtattaaact
     8221 ttattatcgc agtgttttgg ttgtttgtga caaatagtta cacaacaaat aactttgtgt
     8281 taggtttcat ctttggccta gttcttgttt acctcttaca cagagtgtta ccaggtagat
     8341 tttatgtcat cacgctatat cgtattatta aattagttat tattttctta atcgaactga
     8401 ttaaagctaa ttttgatgtt ttgaaaatta ttattaaacc ttcgattaaa aatgaaccag
     8461 gattctttgt ttatcacaca gatttaaaaa aagattggca aattgtcttg ctgtctaatt
     8521 taattacatt aacacctggt actgtagtgc ttggtgtaag tgacgatcgt acaaaaattt
     8581 atattcatgc catagatttt tcaactaaag agcaagaagt agaaagtatt aaaacatcat
     8641 tagagaaaat tgtaagagag gtgggcgaaa tataatgaat cataatgtta ttatcgttat
     8701 tgcattaatc atagttgtca tttctatgtt agctatgctc attcgcgttg tgctaggccc
     8761 atcacttgcc gatcgtgttg tcgcattaga tgcgattggt cttcaattaa tggcagttat
     8821 agcattattc agtattttat taaatattaa atacatgatt gtcgttatta tgatgattgg
     8881 tatattagct tttttaggta ctgcagtatt ctctaaattt atggacaaag gtaaggtgat
     8941 tgaacatgat caaaatcata ctgattagtc ttgcacttat ctttgttatc atcggtgctt
     9001 taattagcgc cctagcagct ataggattat tgagacttga agatgtatat tcacgtgcac
     9061 atgctgccgg aaaagcatca acattaggtg caatgtcatt actatttggt acgtttttat
     9121 attttattgc tactcaaggt tttgtaaata tgcaattaat cgttgcgatt atctttgtat
     9181 taattacagg tcctctttca agtcatatga ttatgaaagc agcatataat attaaaacgc
     9241 cttatactaa aaagactaaa gtcgatgaaa tttcggaaga cttaaaagac acaaaattgt
     9301 agattatata acaaaatcat gaatataaat caacaacaaa cagcagtaag atgattccaa
     9361 attaggaatg attttactgc tgttttcttt tgacattgtt acctcttttt caatgatttt
     9421 ttctttgact acagattcgc cctatctaca tatatctctt taatttaatt gcctttcatg
     9481 tcgttatgta ttatgataat aataattata aatcgtaacg attacgtttt aaaaagagag
     9541 aggttttatt atgcattgga caattatcgg cggtggcata cagggaactg caatcgcaca
     9601 aaaactatta tcaagcggat taacaacaga ccgattaaca atcattgacc cacacgaaac
     9661 tttttgccaa aggtttaact catatacaaa tcgaatagaa atgccttatt taagatcacc
     9721 gattgtacat cacgtacatc cacaaccgtt ccatctaaaa caattcgcta aacagcacca
     9781 atatacaaat gctttttatg gtccttatca acgacctgaa ttgacaatgt ttatggatca
     9841 tattgcacat gcttctaaac aatatcaatt agaggattgc ttggttcaag gtttagttca
     9901 aactttagat aaacaagaag acaaatggca tatcaagtta gaagatggac aaattatcac
     9961 tacagattgc gtcgttattg caataggcag tacaaatatt ccgtttatgc ctgacatttt
    10021 aaaagacaaa cagaatgtaa atcatatctt cgagaaagaa cttgatcaag tagtatatga
    10081 taagaccgat catatcgttg gtagcggcat tactgctgca catcttgcac ttaaattgtt
    10141 aaatcatgat aacgataaaa agattcattt atggctaaat aaagatattg aaatacatga
    10201 ctttgatgct gatcctggtt ggttaggtcc gaaaaatatg tcttcatttt taagtactaa
    10261 aagcatgcct gaaagaaatg ccattgtaca acgcgaacgt cataaaggat caatgcctca
    10321 cgaactgtac ttacgcctta aaaaacatat taaaaatggt cgtataaatg tgcataaaac
    10381 acctatcact caaattagtg gtggtgtaat taacactgaa aatgattctg ttccatatca
    10441 acagattatg gttgcaactg gttttgaaca agattttatg tcacaaccac ttattaagca
    10501 attaatacaa aattatgatg cacctatcaa cgaatgtaat taccctgtta tttccgaaaa
    10561 attagaatgg ataccaaatc tatttgtcgc aggatgcttt gcagacttag aattaggacc
    10621 atttggtaga aatgttatgg gtggccgtaa agctgccgaa cgcattgaac aagcatttct
    10681 aaaactacaa caatatagcg cataaaaaaa ctgctagccg tagttatgaa tatctcataa
    10741 catttcagct agcagttctt ttattttaaa ggtttaatag caactgtacc acgcataact
    10801 gtaattaatt gttctttgtc attcttaatt tttatatccc atacatgtgt cgacttacct
    10861 cgatgaataa tttcagctgt cgcagtaaca cgaccatctt tagcagaatg aatatggtta
    10921 gcattcatct ctaaacctaa tggaataaat ttggttgtat caattaaatt agcagatcct
    10981 aatgaacatg ctgtttcacc taaagcaatc gaagccccac catgtaaata tccaaatggt
    11041 tgttttactt tatcagtaac aggcatagaa ataacaacta aaccatcttc ctggtgatct
    11101 attgacatct caaatgtctc taataaatga gtcatatttt cacctcttta tgcttatagt
    11161 accatagcag caatagtacc gaaaatcatt aaaggaatat tataaaacaa gaagtttggt
    11221 acacatgtat cacgtatatg atcatgttgg ccatcaacat ttaatcccgc agttggtcct
    11281 aatgttgaat cacttgcagg cgaacctgag tcacctaatg cactcgctgt tccaatcaat
    11341 gcgattaatg ccattgtatc tagtccaatt gacgctccaa aaggaatgaa taatgatgcg
    11401 ataataggaa ttgttgcaaa tgatgatcca atacctaaag tgacaattaa acctatcaca
    11461 tacatcatga taatgctaaa taatttatta tcaccagtaa tacttgttaa agttttaact
    11521 aattcatcta tatcaccagt agcattcatt acaccagcaa atccatttgc tgttaaaata
    11581 actacaccaa tataagccat aattttaata ccttcaacaa acttagcatc taattcatac
    11641 caattatatg cacgtgaaat aaagaatacg agtacccctg ccagtgcacc aaaaatcatt
    11701 gaatctgtaa atgtttgtac taaaaatgta gctagtattg ctacaattgt tactattaag
    11761 atatatggtt ttaactctgt aacattgtca ctatctgaaa ttttacgtgt ttcatattca
    11821 cgtggtttac gatatacata taaaccgata agtaagccaa caatataccc cattgaagga
    11881 ataagcattg ctttccaaat catattaaac tcaattgggt gatttgcctt tgcaaagcca
    11941 ctttgaataa tttgctggaa aatttgaccg aatccatatg gtaataacac atacgggaaa
    12001 cataaaccaa aaccgataat caaaccgatt aaacgtctat ctatttttaa gtcattaaac
    12061 agacttaaca atggtgggat gacaattgga atgaatgcaa tatgtacagg aattaagttt
    12121 tgactcatga tactcatagc taataatgca atgattattg tcactttgac tttaactctt
    12181 gaccatcgac tattttcagc gtgaattgca tttataattt ttcctactaa atagtctgtg
    12241 ataccactgt atgaaattaa tgctgcaaat ccacctaata aagcatagct taatgctacc
    12301 tcagcaccat cgactatatt tttcccaaat acatttataa ctttttcaat gctcatgcct
    12361 gaaattaagc caccaactag cgcactgata aataagctta taactacgtt taatcgacat
    12421 aaacatagca caatcattaa aattactgct attactactg catttatcat atgacaacca
    12481 tcctttatca ctctatcgaa ctaaagcgtt tatagactac atagtatcag tagctttaac
    12541 acctgtcaat agcaaactat accaataatc tttatttttt agttaattgt ctttcttaaa
    12601 ataattttag ctttcattaa attaaacaat tttacaagct tggaacacca atcaaaatcc
    12661 taagttctaa aatgcaatat tagtagtcgt tgactgaatg aacatatgct tataatattt
    12721 ttttgcaatg ctagtcaagt tgatttatgc tcacaaggat atgcgattta tattttctta
    12781 caacaatgaa aatgcctgat acaatgcgat cctttatact ttcaagagtt aaattagaaa
    12841 tgtatatcaa gacacaagtt gtatcaggca aataattaaa ttattgttgt tttaaccatt
    12901 gtactatcgt cggaatcata aatcctgttg gcccttttgg accattatat gaagctttat
    12961 tagtcgttgc tggacctgca atatcgaaat gaatgtgagg tgtttgacca ctaaaatgtg
    13021 ttacaaaact tgccgcaaat agcgctttac cttgtccatt cgtatggtta actaaatcag
    13081 cgatatcact gtgtttaata cttgcacgtt cggttgcagt aatcggtaat tcaaatacca
    13141 tttcatcgac ttcagaactt atttgtaata tatcgtttaa tatcacttta ctattcgatt
    13201 caaatgcagc agctttatca tcgcctagtg caacaattgc tgcacccgtt aatgtagcaa
    13261 agtccataat cacactaggc tgatattgat ttgcataaaa cacagcatct gcaaggacta
    13321 atctaccttc agcgtctgta ttcattactt ctacagtttc accacttaat gctgtaaata
    13381 catcatctgg cttcattgat gcttcattta tcatattttc agcacacgca agcactccga
    13441 caatatttac aggcagttgt aaacgactag ccgcttcaat gataccaacg acattcgcag
    13501 cgccacacat gtcaaacttc attgtagcca tgccattctt cgttttaata ctataaccac
    13561 cagaatcata cgttatacct ttaccaacta aggcaattgg tgcttcatct ttgtctttgc
    13621 cattatatgt gatggttact aatctcggtt tatgcttact acctttacct actgcttgta
    13681 aaagtccgaa tccttcagaa actaaagtgt cataatcttt aacatctact ttgacctttg
    13741 tatttttaaa atgattaaca atatcttctg caaatgtttg tggtgttaat acattcggtg
    13801 gcatattact aaagtctctt gccaaattaa tggattggcc aatactgata ccctcatgaa
    13861 taaaatctaa ttcaattaat gattcactaa taaggtttaa attcgtctta aacggtgcct
    13921 tcttacttga tttataatga tcgaattcat atgttgcacg ctcactttga ataccgcatg
    13981 ccattaatac atctgataac tgatcatatt ttgaaataaa tgaatccata agtaaatacg
    14041 tatcttcaat gtgttctgac tttatgtatt ggaaaagatg tccccatatt ttcaacatat
    14101 cttgataact acgtgtcttt aagtttccta aaccaactgt aattaaacga tatgtttgat
    14161 cttgtacatc aaatgcagtt gtataaatct tcccaacttt actaccaata atatgttgat
    14221 gttttagtct ttcaagtgat tctgtaatat cgatatgatt aaaactaatg cgctctaact
    14281 gatttaaatg ttctggtata ccaataatca atgtatttat ttcgttgctt agtgtgttat
    14341 ttaatttaaa attcataatg tacctcctta gatttgatat gttactcact tcaaattgta
    14401 caacaataaa gcccttcagt gacactgaag gactttataa atgaataatt taattttatg
    14461 tgtttagctg aaactttatt taaagcctag aatttacctt ttttgaatgc taaaccgata
    14521 ccaccgattt tgaataccgc acgtgtatca atcacttttt tcatgaatgc tgctttttta
    14581 ccagcgatag gtttaccaaa taccatacct acaccgtcat gtgaacctaa agaacaaaca
    14641 gttccacgat caacgtattc gaattcttca gttgattcac cgtttaagat gcgtttaatg
    14701 tttttagcaa cactttcacc ttgttgcatt gcaatttgtg ctgtagttgg taatggacgt
    14761 tcttctccag ctgggataaa cgctgaacag tcaccaataa caaaaatgtt gtcgtaacca
    14821 ttgattgtta aatcttgctt tgtaacgata cgtccacgtt taacgccttc aaatgattct
    14881 tccattaatt tactaccacg tacaccagct gcccatactg aagtacctgc atttaattgt
    14941 tgtttttcac catctacttc aactacaaaa cctttttcgt tacaagcaac gattggtgta
    15001 gcaattttaa attcaacacc gcggtcttct aagtagctaa ctgcgtggtt aactaattct
    15061 tctgagaaca ttggtaacat tttaggtgct gcttcaacac aagtgatttt aactttattt
    15121 tgatccacac catatttgct acataattca ggaattctgt ctgttaattc acctaagaat
    15181 tcaacaccag tgaatccagc accaccaact aagatagata aatcgttatc atctttttct
    15241 tttgatgctg catagttagc aaatttgtct tcgatatgac gtgataattc acgtgctgtg
    15301 ataacatttt caatttggaa agcatgatct ttcatacctt cgatgccgaa tgtttcacta
    15361 acgaaaccta atgctactac taaaatatca aagtcataaa taccttgatt tgtttctacc
    15421 tttttagcat cacggtcaat ttttgttact tctgcttgaa caaagttcac tttgtctttc
    15481 ttcaagacac tttccacagg atataataca tcttcatagt ttagtgtacc tgctgatgct
    15541 tcatgtaacc atgttgcttc atagtgatat tcatttttat taataagcgt aatttctgct
    15601 tcttctgttg atatcgcttt ttgcaattta gttacagttt gtaaacctgc ataaccagca
    15661 ccaagtacaa gtactttttt acgatcttga gccatttaat ttcacctaag ctttcatatt
    15721 tttttaacca aatgctgata attactagta acaactggtc attactactt ttaccacaat
    15781 gtatactagt ttatcatcat tt
//
LOCUS       DAKTLH010000054        15564 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.054, whole genome shotgun sequence.
ACCESSION   DAKTLH010000054 DAKTLH010000000
VERSION     DAKTLH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15564
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.054"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            33..524
                     /locus_tag="O0394_001402"
     CDS             33..524
                     /locus_tag="O0394_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCY0499486.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            598..1956
                     /locus_tag="O0394_001403"
     CDS             598..1956
                     /locus_tag="O0394_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499487.1"
                     /translation="MTQQQNDKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQAEADKSVAVSNKESKAVALKAQQAAI
                     KEEASANNLSDTSQEAQEIQEAKKEAQAETDKSAAVSNEEPKAVALKAQQAAIKEEAS
                     ANNLSDISQEAQEVQEAKKEAQAEKDSDTLTKDASAAKVEVSKPESQAERLANAAKQK
                     QAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVNIDNKDTVKQK
                     EAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQKKQHN
                     KKSSQGAKKQSSSSKSTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKAKSKIEKRTF
                     ND"
     gene            2091..2195
                     /locus_tag="O0394_001404"
     CDS             2091..2195
                     /locus_tag="O0394_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499488.1"
                     /translation="MRSFHVFMLFETSLDNNSDIAHFKLKMEKYSIIK"
     gene            2656..3747
                     /locus_tag="O0394_001405"
     CDS             2656..3747
                     /locus_tag="O0394_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HCY0499489.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLADLYNAKKLK"
     gene            4101..4214
                     /locus_tag="O0394_001406"
     CDS             4101..4214
                     /locus_tag="O0394_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499490.1"
                     /translation="MKKVYIKLKYIVRYHLAFVFISSFSLNFSKQKYVTVN"
     gene            4287..5276
                     /gene="ccpA"
                     /locus_tag="O0394_001407"
     CDS             4287..5276
                     /gene="ccpA"
                     /locus_tag="O0394_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCY0499491.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGSITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(5374..6543)
                     /locus_tag="O0394_001408"
     CDS             complement(5374..6543)
                     /locus_tag="O0394_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HCY0499492.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(6568..7200)
                     /locus_tag="O0394_001409"
     CDS             complement(6568..7200)
                     /locus_tag="O0394_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0499493.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTHEDLKALTFDAHLTAFRD
                     AEDQYKALLEITTLPEGRIYVARQDQLIVGYVTFHYPDEIERWSTGKLPYLIELGAIE
                     VSINFRQLQLAEKLIQLSLSTPEFENYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            7369..9075
                     /gene="acsA"
                     /locus_tag="O0394_001410"
     CDS             7369..9075
                     /gene="acsA"
                     /locus_tag="O0394_001410"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCY0499494.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRIFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            9515..11182
                     /locus_tag="O0394_001411"
     CDS             9515..11182
                     /locus_tag="O0394_001411"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCY0499495.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVEGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKVREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWKKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            11478..14165
                     /gene="harA"
                     /locus_tag="O0394_001412"
     CDS             11478..14165
                     /gene="harA"
                     /locus_tag="O0394_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HCY0499496.1"
                     /translation="MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKNYPAADESLKDAIKDPALENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVVTNDQSSSVASNQTNTNTSNQNTSTINNANNQPQATTN
                     MSQPAQPKSSTNADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVA
                     DTDVAENSSTATNPKDASDKADVIEPESDVVKDADNNIDKDVQHDVDHLSDMSDNNHF
                     DKYDLKEMDTQIAKDTDRNVDKDADNSVGMSSNVDTDKDSNKNKDKVIQLNHIADKNN
                     HTGKAAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLS
                     QSKMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            14296..14388
                     /locus_tag="O0394_001413"
     CDS             14296..14388
                     /locus_tag="O0394_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499497.1"
                     /translation="MKIILLLFLIFGFIVVVTLKSEHQLTLFSI"
     gene            complement(14368..15273)
                     /locus_tag="O0394_001414"
     CDS             complement(14368..15273)
                     /locus_tag="O0394_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCY0499498.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
BASE COUNT         5809 a         2240 c         2845 g         4670 t
ORIGIN      
        1 cattattaca tgttaattag gaggcgtttt taatggattg gattttacca attgctggaa
       61 ttatcgctgc gattgcattc ttaattttat gtatcggtat cgtagctgta ttaaattctg
      121 ttaagaaaaa cttagattat gttgcaaaaa cacttgacgg tgtagaaggt caagttcaag
      181 gtattactcg tgaaacaaca gatttacttc ataaagtaaa ccgtttaact gaggatatcc
      241 aaggtaaagt agatcgttta aactcagttg tagatgctgt taaaggtatc ggtgactcag
      301 tacaaacgtt aaacagctct gtagatcgtg taacaaattc aattacacat aatatttctc
      361 aaaatgaaga taaaatctca caagttgttc aatggtcaaa tgttgcaatg gaaattgcag
      421 acaaatggca aaatagacac taccgtcgtg gaagtgcaaa ttacaaagct aataatgtag
      481 caactgatgc aaatcatagc tatacttcta gagtagataa ataattaata attaaacaaa
      541 tgcacttaaa tgaggttgtt acttatttaa gtgcatttgt tataggagga tatacaaatg
      601 acgcagcaac aaaatgataa aagaacatta aaaaataaac acacttatca aaatgaacca
      661 ctaccaaacc gtaaagattt tgttgttagt tttataactg gcgcgcttgt tggttcagct
      721 ttaggtttgt attttaaaaa taaagtttat caaaaagcag atgatttaaa agtcaaagaa
      781 caagaactgt cgcaaaagtt tgaagaaaga aaaacgcaac ttgaagaaac ggttgcctat
      841 acaaaagaac gtgttgaagg atttttaaac aaatctaaaa atgagcaagc ggcattaaag
      901 gcacaacaag cagcgataaa agaagaagca agtgcaaata atttaagtga tacatcacaa
      961 gaagcacaag agattcaaga agctaaaaga gaagcacaag cagaagcgga taaaagtgtg
     1021 gctgtatcaa ataaagaatc aaaagcagtg gcattgaaag cacaacaagc agcgataaaa
     1081 gaagaagcaa gtgcaaataa tttgagtgat acatcacaag aggcacaaga gattcaagaa
     1141 gctaaaaaag aagcacaagc agaaacagat aaaagtgcag ctgtatcaaa tgaagaacca
     1201 aaagcagtgg cattgaaagc acaacaagca gcgataaaag aagaagcaag tgcaaataat
     1261 ttaagtgata tatcacaaga ggcacaagag gttcaagaag ctaaaaaaga agcacaagca
     1321 gagaaagaca gtgacacatt aactaaagat gcaagtgcag caaaggtaga agtatcaaaa
     1381 ccagagtcac aagctgaaag attagcaaac gctgcaaaac agaagcaagc taaattaaca
     1441 ccaggttcaa aagagagtca attaactgaa gcgttatttg cagaaaaacc agttgctaaa
     1501 aatgacttga aagaaattcc tcaattagtt actaaaaaga atgatgtatc agagacagag
     1561 acggttaata tagataataa agacactgtt aaacaaaaag aagctaaatt tgaaaatggt
     1621 gttattacac gtaaagctga tgaaaaaaca actaataata cagctgttga caagaaatca
     1681 ggtaaacaat ctaaaaaaac aacaccttca aataaacgaa atgcatcaaa agcatctaca
     1741 aataaaactt caggtcagaa aaagcaacat aataagaaat catcacaagg tgcaaagaaa
     1801 caaagtagtt caagtaagtc aactcaaaag aataatcaaa ctagtaataa gaattcaaaa
     1861 acaacaaatg ctaaatcatc caatgcatca aaaacgccaa atgctaaagt tgagaaagct
     1921 aaaagtaaaa tagagaaacg tacattcaat gactaagatt actaaaatcg ttgaataatc
     1981 aatcatagca gtaagctatg taatatattt ttaaaaattg aatttcaaaa taaaaggtag
     2041 gcattcaggt gcttaccttt tttgatatca tcagtttatt ttgaaaggca atgcgatcat
     2101 ttcatgtatt tatgttgttt gaaacatcgt tagataacaa tagtgatatt gcacatttta
     2161 agctgaagat ggaaaaatat tcgataatca aataaacaat gaattttaga aggtacaatg
     2221 acgttcacta atttaaatat agctgaatgt gttggtgagt gatgttcact atagatttat
     2281 attaatatac aaagacaaag gttgttaatt tttattaagc gttaggttga atgtatgaga
     2341 attttagatt tataatagaa gatagaaacg aaaatttttc ttaaaagcag taatgttgac
     2401 tcaaaataag ctataataat gacacttatt taattgatta acatttgcta ataaatatca
     2461 atatagaata taactttcca ataatgactg agaaaatcga aatgtcagtc tcgaatcata
     2521 taattagaaa attgattatt ttctgtcaat ttagggttga actatacata tgatattgtt
     2581 agaatatttt ttaacattat attttattgc tttaaagtgg aatatacttg aaataattag
     2641 tagaggtgag taaggatgag taataaatta gaatcataca gaagtgagat tgtatcactg
     2701 aatcatcaaa ttttagactt attatctaaa cgtggtgaac tagcacaaaa aattggggaa
     2761 gaaaaattaa aacaaggtac acgtatttat gatccacaac gtgaaaaaga aatgcttaac
     2821 gacttaatcg atagtaacaa aggaccattc aacgataata ctattaagca attatttaaa
     2881 gaaattttca aagcctctac agatttacaa aaatctgaaa atgaaaaaca tttatatgta
     2941 tcacgtaagt tgaaacctga agatacgatt gtaacatttg ataatggggg cattattgga
     3001 gacggcaata aatcatttgt atttgggcca tgttcagttg aatcatttga acaagttgaa
     3061 gctgttgcta aaaacttaca tgctaaaggt gaaaaattta ttcgtggcgg tgcatttaaa
     3121 ccacgtacat caccatatga tttccaaggc ctaggtgttg aaggacttaa aatacttaaa
     3181 cagattaaag ataaatatga tttaaatgtt gtcagcgaaa tcgtaaatcc aaatgatttt
     3241 gaagtggctg atgagtattt agacgtattc caaattggtg cacgtaatat gcaaaacttc
     3301 gagttattaa aagaagctgg ccgtacgaaa aagcctattc tattaaaacg tggtttatct
     3361 gctacaatcg aagagtttgt ttatgcagct gaatacattg cttcacaagg taatcaaaac
     3421 attattttat gtgaacgtgg aatccgaact tatgaaaagg cgacacgtaa cactttagat
     3481 atttcagcag taccaatttt aaaacaaggt acacacttac cagtcatggt agatgttacg
     3541 catagtacag gtcgtaaaga tatcatgtta ccaactgcga aagcagcatt agcagttggt
     3601 gctgatggag ttatggctga ggtgcatcca gatccatctg ttgcacttag tgatgcgggt
     3661 caacaaatgg atttagatga attccaagca ttttatgatg aattaaagcc tttagctgat
     3721 ttatataacg ctaaaaagtt aaaataatat tccaaggaaa ctatagacta cttaactaat
     3781 atgtcatgtt gaagtagaat attatctttg aatcgacaat tttaaactta cagccattct
     3841 aagagtatat tacttttaga gtggctatta ttttttgtat agaaataaag gtatactgca
     3901 cttaacgatt gttataatac ttcgacactt gttcaatttc acaattatta aagattatga
     3961 ctgatagcag taattaaaat tataactatg aattatctgt aaaatataat agattcacac
     4021 atttgttgct gaaatgtgaa catttttcaa caaatgcaat tgatatttga aaaggctttc
     4081 tcaaaacatt acaattaaaa atgaaaaaag tttatataaa attaaaatat atcgttcgtt
     4141 atcatttagc gtttgttttt atttcaagct tttcgctaaa tttttccaaa caaaaatatg
     4201 ttactgtaaa ttaaaatatg gtaaactatg aaaatgaaat gaaaacatgt tattataatg
     4261 aataaaacgt ttacaaggag gaaattatga cagttactat atatgatgta gcaagagaag
     4321 cgcgtgtctc tatggccaca gtgtcgcgtg ttgttaatgg gaaccaaaat gttaaagcag
     4381 aaactaaaaa taaagttaac gaagtcatta agcgtttgaa ttatcgtcca aatgctgttg
     4441 ctagaggttt agctagtaaa aagacaacaa cagtaggtgt gatcattcca gatatatcta
     4501 atatctatta ttcacaactt gctcgtggac ttgaagatat tgcaacaatg tataaatatc
     4561 actcaattat ttcaaattca gataacgatc ctgaaaagga aaaagaaatt tttaataact
     4621 tattaagtaa acaggttgat ggtattattt tccttggtgg ttcaattact gaagaaatga
     4681 aagaattgat aaatcaatca tctgtacctg tagtagtatc aggaacaaat ggtaaggatg
     4741 cacatatagc atcagttaat attgatttta ctgaagctgc gaaagaaatt acgggagaat
     4801 taattgaaaa aggcgctaaa tcatttgctt tagtaggtgg agaacattct aaaaaagctc
     4861 aagaagatgt tttagaaggt ttaactgaag tgttaaataa aaatggcctt caattaggtg
     4921 atacattgaa ttgttctggt gctgaaagtt ataaagaagg cgtaaaagct tttgccaaaa
     4981 tgaaaggcaa tttgccagat gccattttat gtatcagcga cgaagaagca attggtatta
     5041 tgcatagtgc aatggatgct ggtattaaag ttccagagga attacaaatt attagtttca
     5101 ataatacacg attagttgag atggttagac cacaactttc tagtgttatt caaccattat
     5161 atgatatcgg tgcagtaggg atgcgcttat taacaaaata tatgaacgat gaaaagatag
     5221 aagaaccaaa tgtagtttta cctcacagaa ttgaataccg aggaactaca aaataaattc
     5281 acaaaattag gcattcatct aacgacccaa attatatggg tgttggaaga atgcctttta
     5341 tttatctttt aaaatcgttg cagattaggt tacttattga cgagtagatt cgtaccaact
     5401 cgctatatgt aaagctaatt ttttattttt ttcactaatt tcttttgtgc gggggacata
     5461 ggtataatca tttaaacgat cttcccatct tttaggtaat aattcagatg aataatgttt
     5521 ccatttatta atccattcta acggtaaata accactttga attggttgat caattaaact
     5581 taagaataca tgactccatg cacgtggtac gactctccaa atattgtagc ctccgccacc
     5641 aaacataatt acctttccat tcgtataaga atcagctaaa tattttacaa aatatggaat
     5701 ttcatataat gaatgtaacg tacaatttag atgagttagt ggatcacgat aatgtatatc
     5761 gacaccattt acgcttagaa taatatcagg tttaaaactc tttacgacag gctcaactgt
     5821 taatttaaaa cactccaaaa atgatgcatc ttctgtatac ggttcaagtg ggacatttac
     5881 agtgtgtcca tagccgatat cttcaccgcg ctcagtatag tgaccagagc ctgggaaaag
     5941 aaattttccg gtttcatgga tagaataagt agtaacatgg ttatcggcat agaaactcca
     6001 ttgtgtacca tctccatgat gtgcatcggt atctatgatt aaaacgcgtt gattgtattc
     6061 tttagctaag tattgtgcgg taattgcaat atcattgtat atacaaaaac cacttgctcg
     6121 accaggttga gcgtgatgca aaccaccacc taagtgacaa ccatttaata ctttgcctga
     6181 cataataaga tctgctaaag ttaaagcgcc tccaacaatt gtggcactat ggcggtgcat
     6241 atgcttaaat tgaccattct cttcatcatt taatccatat ttcttagcct catcttcact
     6301 gataatgcca tgtgaagcat gcttaatagc ttcgacgtaa tcatatttat gaattaacat
     6361 taattcgtca tctgttgcaa ttctaggttg tactatttgt tctggagaca ataaatttgc
     6421 attcaaaagt agctctgttg ttaattttaa acgcatttga ttgaagggat gttggtcatg
     6481 aaatcgatat tgtaataact tatctgaata aacatatgca gtttttgatg aatgttgttg
     6541 catataatcc ctccgatatt ccaaaaatta aaagaaaaac cgattcatat aacgaatatc
     6601 atcaaacgct tgttgctgtt ctaatgtaat gtttttgcca attcttgcca ttaaacaatt
     6661 agctggatga cttgttattt ctggatcatc tgtagcgaat atttcaagtc caccagttgc
     6721 cattaaccgc tgcattaatt ttttatagtc aaatacatct aactttgaat tttttaaatc
     6781 ccaatgccag taatattctg tagttataac gatataattc tcgaattctg gtgtagaaag
     6841 gctaagttgt atcagctttt ctgcaagttg taattgccta aaattgatgc tgacttcaat
     6901 tgcccccaat tcgattaaat atggaagctt acctgtagac cagcgctcaa tttcatcagg
     6961 atagtggaaa gtgacataac ccacaatgag ttgatcttgg cgagcaacat aaattctacc
     7021 ttctggtaat gttgtaattt ctaacaaagc tttatactga tcttcagcat ctctaaatgc
     7081 ggttaaatgc gcatcgaaag taagcgcttt caaatcttcg tgtgttaaag gaccttcaat
     7141 aacaaattgc ttgtcatgaa tgtaataatc ttcggattga tacgtcttta aatgattcat
     7201 attttcaact cctcaatcga cgtcgtgatt gtattaaatt cattatatag aaaatttaca
     7261 ataattaata ctagaaaaag gataaaagta aaaattttga ataattagaa atgttatgta
     7321 taatattgag aaagaaagcg ttttcacata acaaaggggg agtttcaaat gaaagtcgaa
     7381 gtttataaag gagcgcaagg taaacataac cttaaagatt atgaagaaac atataatact
     7441 tttgattgga aagacgtaga acaagcattt tcttggagtg aaactggaaa aatgaacatg
     7501 gcatatgaat gcatagatcg ccatgtagat caaggattag gggataaaat agcgttaaat
     7561 tacaaagatg agcacagaaa agaatcgtat acttataaag atatgcaacg gttatctaat
     7621 aaagcagcga atgttttgtc tgaacatgca gaagttgaca aaggtgacag agtatttata
     7681 tttatgtcgc gtacacctga actatatttt gcgttgttag gtgttttaaa aattggtgca
     7741 attgttgggc cgttatttga agcatttatg gaaaaggcag ttgcggatag attagagaac
     7801 agtgaagcta aagtgttaat tactaataag gcattgttac ctcgagtacc tgtagataaa
     7861 ttaccaaact tgaaaaaaat tgttgtcgta gatgaggatg tagaagacaa ttacatagac
     7921 ttcattagtt tgatggaaac tgctagcgat gaatttgaca ttgaatggtt aaagtcggat
     7981 gatggtttga ttttacatta tacatcaggt tctactgggc aacctaaagg tgtattgcat
     8041 gttcaacaag caatgttagt gcactatatt tctggaaaat atgtattaga tttacaagaa
     8101 gatgatgttt attggtgtac agcagatcca ggttgggtta caggaacatc ttatggtatt
     8161 tttgcaccat ggttaaatgg cgctacaaat tgtatagctg gtggtcgctt ttcgccagaa
     8221 cagtggtata gtatgattga agattttaaa gtgacgattt ggtatacggc accaacagct
     8281 ttaagaatgt taatgagtgc tggtgacgat attgttgaga aatatgactt gtcatcgtta
     8341 cgttcgattc tatcagtagg tgagccttta aatcctgaag ttataaaatg ggcgaaaaaa
     8401 gtatacggtt taacggtgtt agatacttgg tggatgacag aaacaggtgg acatatgatt
     8461 gttaactatc caacgatgga cgtcaagctt ggctcaatgg gcaaaccatt acctggtatt
     8521 caagctgcaa ttatcgatga tgcagggaat gaattaccac caaatcgaat gggcaacctt
     8581 gctataaaaa aaggctggcc atcaatgatg tatcgtatct ggaagaatcc agaaaaatat
     8641 aaatcatatt ttattggaga ctggtatgta tctggtgatt cggcatataa agatgaagat
     8701 ggttacttct ggttccaagg acgtgttgat gatgtaatta tgacagctgg tgaacgagtt
     8761 ggaccatttg aggttgagtc taaattggtt gaacacgaag cagttgccga agcaggaatt
     8821 attggtaaac ctgatccggt tcgcggtgaa ataattaagg cgtttgttgc actgagaaaa
     8881 ggatatgaac caacagacga attaaaagaa gaaattcgta tatttgttaa agaaggtttg
     8941 tcggcacatg cagcaccacg tgaaatcgaa tttaaagata aattacctaa aacacggtca
     9001 ggtaaaatta tgagacgtgt attaaaagct tgggaattaa atttagatgc tggggattta
     9061 agtacaatgg aataatgaca tgaatgttat tgaagatttt tttcgaagaa taaagggtga
     9121 caacatattt catgtcaatg tttaaataat cgtttacttt acgataagca atataaagaa
     9181 ctgttaactt gtgtcatatc atttcgtaga aagcatttga aaatgatgac ataacaataa
     9241 tggcatatct ttatattgct ttttattttt aatatgatct ttggaagatg attattttaa
     9301 ataatagaaa aatatagtta tcaatagtat caagcgctaa aagttgtata atacaaaact
     9361 ttaataagtg aatttattgc aaaaatgaaa gcgctaaccc gatttagtcg acaagttttt
     9421 aacagttcgt tattatatga atgtaagtaa aaatttctta gctacaactt acatattata
     9481 aatgcataaa ttaaacaaaa aggggcgaaa aaagttgact catttatcag atttagatat
     9541 tgcgaatcaa tcaacactac aaccaattaa ggatattgct gcatcagtag gtatttcaga
     9601 ggatgcatta gaaccttatg gtcattacaa agctaaaatc gacattaata aaattacgcc
     9661 aagagaaaac aaagggaaag ttgttttagt aactgcgatg agcccaacac cagctggtga
     9721 aggtaaatca acggttacag ttggtttagc tgatgcattc catgagttaa ataaaaacgt
     9781 tatggttgca ttaagagagc ctgctttagg accaacattt ggtatcaaag gtggtgcgac
     9841 tggtggtggt tatgcgcaag tcttacctat ggaagatatc aacttacatt tcaacggaga
     9901 tttccatgcg attacaactg caaataatgc attgtctgcg tttatcgata atcatattca
     9961 ccaaggtaac gaattaggaa tcgatcaaag acgtattgag tggaaacgtg tattagatat
    10021 gaatgatcgt gcacttagac atgtaaacgt tgggttaggt ggacctacaa atggtgtacc
    10081 acgtgaagat ggctttaata ttacagtagc gtctgaaatt atggcgattt tatgtttaag
    10141 tagaagtatt aaagacttaa aagataaaat tagtcgtatt actattggtt acactagaga
    10201 tcgcaagcca gttacagttg cagatttaaa agtggaaggt gcacttgcaa tgattttaaa
    10261 agatgcaata aaaccaaact tagtacaatc aattgaaggg acacctgcat tagttcatgg
    10321 tggaccattt gcgaatatcg cacacggttg taactcaatt ttagcaactg aaacagcacg
    10381 tgatttagct gatatcgttg taacggaagc tggatttggt tcagacttag gcgctgaaaa
    10441 attcatggac attaaagtgc gtgaagcagg atttgatccg gcagctgtcg ttgttgttgc
    10501 gacaattcgt gcgttaaaaa tgcatggtgg tgtagcgaaa gataatttaa aagaagaaaa
    10561 tgtagaagca gtaaaagcag gaattgttaa tttagagcgt catgttaata atattaaaaa
    10621 attcggtgta gaaccggttg ttgcaattaa tgcatttata catgataccg atgcagaagt
    10681 agaatatgta aaatcttggg ctaaagaaaa taacgtacga attgccttaa ctgaagtttg
    10741 gaaaaaaggt ggtaaaggtg gcgttgactt agcaaatgaa gtattagaag tcattgatca
    10801 acctaattca tttaaacctt tatatgaatt agaattacca ttagagcaaa agattgaaaa
    10861 gattgtgact gaaatctatg gcggttcaaa agtaacgttt agcagtaaag cgcaaaaaca
    10921 attaaaacaa tttaaagaaa atggttggga taattaccca gtatgtatgg cgaaaacaca
    10981 atattcattc tcagatgatc aaacgttgtt aggtgcacca tcaggatttg aaattacaat
    11041 tcgtgaatta gaagcgaaaa caggtgcagg atttatcgta gcgttgacag gtgcaatcat
    11101 gactatgcct ggtttaccta aaaaaccagc agcattaaac atggatgtta ctgatgatgg
    11161 tcatgcaatt gggttattct aataaatcat gtcaattgtt taataaagat aagtaaatag
    11221 tttaatagac cggactgttg gagatgcatt atttcagcag ttcggttttt tgctgtgcta
    11281 aaaatagatt caatttggcg aatctaacga caatgtttga aggtggttaa ttaatgtata
    11341 tgaagataaa aagtgggctt gaagaatagg aaagcgatgc aatgaatatt ccatattaaa
    11401 aaaaaataat aaaataggtt gcaatattta attgggatgc gctacaatta acactaataa
    11461 taggagttgt taacaacatg aacaaacatc acccaaaatt aaggtctttc tattctatta
    11521 gaaaatcaac tctaggcgtt gcatcggtca ttgtcagtac actattttta attacttctc
    11581 aacatcaagc acaagcagca gaaaatacaa atacttcaga taaaatctcg gaaaatcaaa
    11641 ataataatgc aactacaact cagccaccta aggatacaaa tcaaacacaa cctgctacgc
    11701 aaccagcaaa cactgcgaaa aactatcctg cagcggatga atcacttaaa gatgcaatta
    11761 aagatcctgc attagaaaat aaagaacatg atataggtcc aagagaacaa gtcaatttcc
    11821 agttattaga taaaaacaat gaaacgcagt actatcactt tttcagcatc aaagatccag
    11881 cagatgtgta ttacactaaa aagaaagcag aagttgaatt agacatcaat actgcttcaa
    11941 catggaagaa gtttgaagtc tatgaaaaca atcaaaaatt gccagtgaga cttgtatcat
    12001 atagtcctgt accagaagac catgcctata ttcgtttccc agtttcagat ggcacacaag
    12061 aattgaaaat tgtttcttcg actcaaattg atgatggaga agaaacaaat tatgattata
    12121 ctaaattagt atttgctaaa cctatttata acgatccttc acttgtaaaa tcagatacaa
    12181 atgatgcagt agtaacgaat gatcaatcaa gttcagtcgc aagtaatcaa acaaacacga
    12241 atacatctaa tcaaaataca tcaacgatca acaatgctaa taatcaaccg caggcaacga
    12301 ccaatatgag tcaacctgca caaccaaaat cgtcaacgaa tgcagatcaa gcgtcaagcc
    12361 aaccagctca tgaaacaaat tctaatggta atactaacga taaaacgaat gagtcaagta
    12421 atcagtcgga tgttaatcaa cagtatccac cagcagatga atcactacaa gatgcaatta
    12481 aaaacccggc tatcatcgat aaagaacata cagctgataa ttggcgacca attgattttc
    12541 aaatgaaaaa tgataaaggt gaaagacagt tctatcatta tgctagtact gttgaaccag
    12601 caactgtcat ttttacaaaa acaggaccaa taattgaatt aggtttaaag acagcttcaa
    12661 catggaagaa atttgaagtt tatgaaggtg acaaaaagtt accagtcgaa ttagtatcat
    12721 atgattctga taaagattat gcctatattc gtttcccagt atctaatggt acgagagaag
    12781 ttaaaattgt gtcatctatt gaatatggtg agaacatcca tgaagactat gattatacgc
    12841 taatggtctt tgcacagcct attactaata acccagacga ctatgtggat gaagaaacat
    12901 acaatttaca aaaattatta gctccgtatc acaaagctaa aacgttagaa agacaagttt
    12961 atgaattaga aaaattacaa gagaaattgc cagaaaaata taaggcggaa tataaaaaga
    13021 aattagatca aactagagta gagttagctg atcaagttaa atcagcagtg acggaatttg
    13081 aaaatgttac acctacaaat gatcaattaa cagatttaca agaagcgcat tttgttgttt
    13141 ttgaaagtga agaaaatagt gagtcagtta tggacggctt tgttgaacat ccattctata
    13201 cagcaacttt aaatggtcaa aaatatgtag tgatgaaaac aaaggatgac agttactgga
    13261 aagatttaat tgtagaaggt aaacgtgtca ctactgtttc taaagatcct aaaaataatt
    13321 ctagaacgct gattttccca tatatacctg acaaagcagt ttacaatgcg attgttaaag
    13381 tcgttgtggc aaacattggt tatgaaggtc aatatcatgt cagaattata aatcaggata
    13441 tcaatacaaa agatgatgat acatcacaaa ataacacgag tgaaccgcta aatgtacaaa
    13501 caggacaaga aggtaaggtt gctgatacag atgtagctga aaatagcagc actgcaacaa
    13561 atcctaaaga tgcgtctgat aaagcagatg tgatagaacc agagtctgac gtggttaaag
    13621 atgctgataa taatattgat aaagatgtgc aacatgatgt tgatcattta tccgatatgt
    13681 cggataataa tcacttcgat aaatatgatt taaaagaaat ggatactcaa attgccaaag
    13741 atactgatag aaatgtggat aaagatgccg ataatagcgt tggtatgtca tctaatgtcg
    13801 atactgataa agactctaat aaaaataaag acaaagtcat acagctgaat catattgccg
    13861 ataaaaataa tcatactgga aaagcagcaa agcttgacgt agtgaaacaa aattataata
    13921 atacagacaa agttactgac aaaaaaacaa ctgaacatct gccgagtgat attcataaaa
    13981 ctgtagataa aacagtgaaa acaaaagaaa aagccggcac accatcgaaa gaaaacaaac
    14041 ttagtcaatc taaaatgcta ccaaaaactg gagaaacaac ttcaagccaa tcatggtggg
    14101 gcttatatgc gttattaggt atgttagctt tattcattcc taaattcaga aaagaatcta
    14161 aataattaac taaatatagc atatgtatga ttaactttgt agacaatgtg aaagcaatta
    14221 atttataaac tattgattgg tttaatggct ttcctttaga gtaaataaaa agaacagcag
    14281 tgagaaattt tctaattgaa aataatctta ctgctgtttt taatatttgg attcattgtt
    14341 gtggttactt taaaaagtga gcatcaatta acgctttttt cgatttaaca aatgtgattt
    14401 aatatcatat tttaatgcgt cgttgtattc tttttcagtg atttgatctt cgattaacat
    14461 acgctttaat acataatgtt gtctttgaat actatatttc aaatctttat ccgattttaa
    14521 cgttccatct ttttcgtagg gtgtatagcc atatgggctt tgcaacaaac cgataaggta
    14581 tgcagattgt gcaattgata aatcttttgg tggaatacca aatagactat atgaagcgga
    14641 tgcaattccg gaaatattag cgccattata atctctaccg aagggaacta tatttaaata
    14701 tgtatatata atttcatctt ttgagagtag gtgttctaat ctaattgcta ggcgaagttc
    14761 atttgctttt ctactatatg ttttttcgtt ggtaagaact tgatttttaa caagttgttg
    14821 tgtaattgtg ctaccacctg aactttgatc agtattaaaa atatcttgta tcattgctct
    14881 taaaatcgcc tttggtaaga tgccatcatg tttataaaat aaagtgtctt cagatgacgt
    14941 taatgcttta atgacatttg gacttgatgt tttagggcct ataatgagtg agttttgaga
    15001 atggttatac tcatataata aatttttgtt attatgatct aataattcat cgccaggtat
    15061 ttgtcgaact ttttttatta aagcatcatc tgataatgag tcggacgttt tagttaaatg
    15121 atgaaaatat aaagacatcg caatcacagc gatagcaacg atggcaatga aaatcataaa
    15181 tataaaaaca atgacatgtt tgatagcttt atatatcttt tcaaaatgat atatacgatg
    15241 attcaattga tgattgttgt cttgattcgt catctttaac ctcctaaatt acgtatagta
    15301 taccatattt aagcaatttg acttttattt taacgttaat tataattagg gaaaatgcat
    15361 aaagaatagg ataaaaggac aagtaatatt atttgaaatc atcagagagc tagtggtcgc
    15421 tgtgaactag tgtttttaat aatatgaata cacctttata actaacatgt cctacgttta
    15481 ttaatgaacg tatcattaat agagtgaaca ttatcttatg attatgttta agtttggtgg
    15541 taacgtgcaa tcgcgccctt acat
//
LOCUS       DAKTLH010000055        15549 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.055, whole genome shotgun sequence.
ACCESSION   DAKTLH010000055 DAKTLH010000000
VERSION     DAKTLH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15549
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.055"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..281)
                     /gene="mreD"
                     /locus_tag="O0394_001415"
     CDS             complement(<1..281)
                     /gene="mreD"
                     /locus_tag="O0394_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCY0499499.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFF"
     gene            complement(281..1123)
                     /gene="mreC"
                     /locus_tag="O0394_001416"
     CDS             complement(281..1123)
                     /gene="mreC"
                     /locus_tag="O0394_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCY0499500.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            complement(1323..1442)
                     /locus_tag="O0394_001417"
     CDS             complement(1323..1442)
                     /locus_tag="O0394_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499501.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            complement(1516..1989)
                     /locus_tag="O0394_001418"
     CDS             complement(1516..1989)
                     /locus_tag="O0394_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HCY0499502.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKSENAYQ"
     gene            2149..2346
                     /locus_tag="O0394_001419"
     CDS             2149..2346
                     /locus_tag="O0394_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499503.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            complement(2463..2747)
                     /locus_tag="O0394_001420"
     CDS             complement(2463..2747)
                     /locus_tag="O0394_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499504.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(2815..3501)
                     /gene="radC"
                     /locus_tag="O0394_001421"
     CDS             complement(2815..3501)
                     /gene="radC"
                     /locus_tag="O0394_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCY0499505.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIKVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            complement(3498..4205)
                     /locus_tag="O0394_001422"
     CDS             complement(3498..4205)
                     /locus_tag="O0394_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCY0499506.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            complement(4475..5746)
                     /locus_tag="O0394_001423"
     CDS             complement(4475..5746)
                     /locus_tag="O0394_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCY0499507.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            complement(5759..8389)
                     /locus_tag="O0394_001424"
     CDS             complement(5759..8389)
                     /locus_tag="O0394_001424"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCY0499508.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     misc_binding    complement(8433..8734)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8792..9352
                     /locus_tag="O0394_001425"
     CDS             8792..9352
                     /locus_tag="O0394_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCY0499509.1"
                     /translation="MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLQDCPSKPKHN"
     gene            complement(9575..10648)
                     /locus_tag="O0394_001426"
     CDS             complement(9575..10648)
                     /locus_tag="O0394_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCY0499510.1"
                     /translation="MAMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKNNWLSIIVVSISILLIAIVMA
                     FVFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYIIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(10682..10783)
                     /locus_tag="O0394_001427"
     CDS             complement(10682..10783)
                     /locus_tag="O0394_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499511.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            complement(10844..12130)
                     /gene="hemL"
                     /locus_tag="O0394_001428"
     CDS             complement(10844..12130)
                     /gene="hemL"
                     /locus_tag="O0394_001428"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCY0499512.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGNDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            complement(12178..13152)
                     /gene="hemB"
                     /locus_tag="O0394_001429"
     CDS             complement(12178..13152)
                     /gene="hemB"
                     /locus_tag="O0394_001429"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCY0499513.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            complement(13155..13823)
                     /locus_tag="O0394_001430"
     CDS             complement(13155..13823)
                     /locus_tag="O0394_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCY0499514.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            complement(13845..14771)
                     /gene="hemC"
                     /locus_tag="O0394_001431"
     CDS             complement(13845..14771)
                     /gene="hemC"
                     /locus_tag="O0394_001431"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCY0499515.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQNEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            complement(14813..>15549)
                     /gene="ccsA"
                     /locus_tag="O0394_001432"
     CDS             complement(14813..>15549)
                     /gene="ccsA"
                     /locus_tag="O0394_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HCY0499516.1"
                     /translation="FVQKSHKIRSLGIYLLGIVWVLQTISLSIFIIQTRHIPLGSISD
                     VFYTLSWLIISISLILNLIKVLNFSVFFLNLIGLTLLGMNTFQPTHYTNKVQKIAVVD
                     ELLLVHIGLAVLSYVFFALAFVNALLYIMQYRNLKEKRFDQKYFRIGSVATLESIVFY
                     STLSGWIILIFSIILGTQWGVISVGERIFIDPKVILSSIITVLYGSYILLRINKWLNS
                     RYLIYYNIILFCLNMINLFFATHFVN"
BASE COUNT         5136 a         2910 c         2109 g         5394 t
ORIGIN      
        1 ttaaagaatc gatctataag tgctaaaaac aatatatagc caaataagta cactccataa
       61 atactaccaa agtatacatc agtcattacg cctaaaaata tgctgagcaa taatgatacg
      121 ccaaagccac gatacactac catcattaaa atatacataa atgtaaggtg tggtacaaat
      181 acaagttcaa acttacctat gtgcattgga ataagaagcc caattgcagt atctatataa
      241 aatagtaaaa tacctatcaa aaaataatac agtgtacgca ttatttatcc ctgctttcat
      301 catcaggaat tgttttagga tctctttttg caacataaac atgactcaaa tctgttaagt
      361 ccgcaccagt cttaacccta acttctttag ctaagccgta ttgatcattt tgaaccttag
      421 tcacttctcc tatatataaa ttacttggta gttgatcagc taatccactt gtaacgactt
      481 tatcaccttt tgagatatta tctctattat taatgtcact aattacaagt tctgagttct
      541 tttcatcata acgatcaatt aaaccaaata tatttttaga accgtgttgt atatttacag
      601 ataatttacc cgcacgtgta ttagttgaga ttaaatcaac ttgtgaagaa aatttattaa
      661 ctttagtaac tcttccaaca aaaccttgtg atgtcatcac agccatattt gaagttatac
      721 ctgatttaga tcccttatca attacaattg tattcatcca ctgatccgga tttcttgcca
      781 aaaccgtagt agaaatagga tcaaattttg aaatatcttt taaatcaagc tcttttttta
      841 atttttcatt ttccgcttct aattgttggt tcttagattc taactggcta atcttatttt
      901 tagattcttt agaatctcct tttttaaaaa agtccccaat cgtaccagca acaaaattaa
      961 ctggataact cacaactcgt tgtccaaaag acacagaatc acctatatat tgttcaggag
     1021 gtgattgaga ttgtgaacgt atggacagcc caattaatgc aataaaaacg ataattgcac
     1081 ataaaacaac aattaatttg ttatttttaa aaaacttaag cacccagaac acctctatta
     1141 tgtcaaaata ttgtatatcc ttttctattt tatattactc ccattatgaa ataaagcact
     1201 aactacgaat tactttgaaa atgaacaact ccgtaaaaaa tttgtggcat tttaaaaaca
     1261 aaaaaactac tagcaaacat tccagtcact agtagtttaa ataatcgatt taaattttaa
     1321 aattatttgt ttgtatcgtt cgaggattca ctgttcgaat tctgaagttg tctccaacgt
     1381 gcgaataagt tctgggcctt ttgttgttca ttagcttgtt tctgttttga ttgttctgcc
     1441 atttgaacta caccaacctt ttaaatttat tctagtgaca ggataactaa aatatatttc
     1501 ttatgcaata atttcctatt gataagcatt ttcagatttt agttgtaaat tttgccctaa
     1561 ttcatttaag tcttgttgca tttcaaattc agtattgtaa acacgcattg actcatctcc
     1621 aaatttataa agaataaatt cgtctcctct ttgacctata atatattgat cattataagc
     1681 catgcgattc attccagaca cagccataaa ctcttgttta tctatcattt taaatacatt
     1741 tttaatttgg cttaatggta cattctttat taaatcattt tcttttaatg aatatttctc
     1801 accacttaca taaacatgtt gggcatcatt agtgggttgc gtaacttgat tcatttgatc
     1861 attatcgtga tttacaggat tccaatcttg ttcttttaaa aaaggtgcgt atttcaaggc
     1921 gatataaaca attatcacaa ttgctattac tgctataatg tttttaatca tactaaatat
     1981 caatctcata gttcgcctcc aatgtcttca tctaattata tattttatta tgtatatttt
     2041 cctcagtctt cagccttaat catgatatat cttaggactg atgcacgttc atttaaaata
     2101 gcttataatt ttgttatgat ttaaaagcgt attaggaggt ttgtagcatt gagtatacta
     2161 acaattattt taatagcact attagtcatc ctccttttta gagttggact ttctattttg
     2221 cgatttttaa tttatgtcgg acttgtttta ttatgtattt acttaggata tcaaggttta
     2281 atttggttgc tcgatttttt tcaaataaat agtggatttt taccgcactt tcaatttaac
     2341 aattaaattt attctgcata acaaaactcc taatgtacta gtttagaatt ttttaactag
     2401 ttccacatta ggagttttag tttgttttca agcacggata ctacttgatg aacagattac
     2461 tattacttac gcccaaatgc gttagataaa aatcgtatca gcgcgtagct accacttagt
     2521 aaaaggcccc aaacgattcc agtaaaaatt gagccaattg ctacacttaa taaaggaaaa
     2581 gcttgtttcg taacgattac acctgctaac gcggttacta aaatacctaa cgtagataaa
     2641 gctaatgtta ttaaaagatt gcgctttgtt acaggttcat ttttaatatt taactgcatg
     2701 gtataaacca ttggcacgag aaccccgata ataataaata gagtcactat ttttcctcca
     2761 atgaaagtac ttcgtaggta ttatacacaa gatacgtcat taattctatc aacttcaatc
     2821 attttcatca aagtaacccg cttctacaag actggtaaat ctattatcac cgattataat
     2881 atgatccaat aaatctatcc ctaaaatcaa accacactcc ttcaacctca ttgttgttat
     2941 gatatcttct tgtgagggcg ttacatcacc ggatggatga ttatgaactg cgatgattgc
     3001 attggcattt tctctcaccg caatactaaa aatttcacgt ggatgtacaa tcgaactatt
     3061 taatgtacct ttaaaaacac aggtttcttt aatcactaca ttttttgaat ttaacaataa
     3121 aatgacaaaa tgttcttgtg ttaaatcttt cattgttgga atcatataat cagcaacatc
     3181 acttggttgc gttattttta tacgattatt ttcagctctt ctccccatcc tttcccctaa
     3241 ctcaaatgct gcttttaaag taattgcttt ttgtaatcca atccctttaa cttttatcaa
     3301 atcgttaatt gaagattttt tcaattcatt cagattcgaa gcagatttaa gcagttcatt
     3361 actaatgtct atgctcgaga atccttttct tccggtgtta attaatatag ctaataattc
     3421 tgtattcgaa agactttttg caccatggct taacaaacgt tctcttggca tttctgaagt
     3481 taccatttct ttaattttca aaaatatacg cctcctaaaa attgatggat atcattataa
     3541 aaaagtgaat tgataaaaaa ggaaataaat ataaatggaa caaggggtaa tagtttaatc
     3601 ggcttaaata tcatggtaat taaagcaact aaaccagcaa tgacaaatgt aaataaaatg
     3661 acataaatag tgaattggag agggaaaaac aaagaaagtg cagatattag taaaacgtca
     3721 ccataaccaa tatatgcccg aaataaaaag tagaatatat gcgtggtcat actaataatg
     3781 ataaaagcta ctggataaat catacttaac gagagagaaa cgatacaata aattataatt
     3841 aagcgacaat ctaacattaa agaagtgata tcggtcatag taaaaataag cagaaaaaca
     3901 tatgtagtta taaatagcgt agcatttacg tatgtgaaat catacttaat aaagacgata
     3961 ggtattaaag caaaggtttc ccctaagaaa tgtgttaggg aaatacgctt tcgacagttt
     4021 cgacatcgcc cttttaataa taaaaaacta ataatcggca ttaattcata ccatttgagt
     4081 gacgaattac aataatcaca tttcgatctt ctatgtaaat aatcaaatga cgtttcttct
     4141 atagatataa attgatatag aaaactaaaa atacaactgc aactataaga taacaatact
     4201 accaaatgac aacctcctta tgtaaattat agttagttat taccaaaatg taaatataca
     4261 ctatttttca agaattgaac cgctttttca tttaaatttt tcaatattgc taagcataat
     4321 tgatggatac tttaacaacc cattactgct cggcaaaatt aataatggca agaaattgaa
     4381 ccttataaac acatacgatt tagagcataa aaaataacca tgaagctcta cctattgatt
     4441 aaatagattc ttcatggcta ttttagtttt agttttataa tgcttcaaag tctaattttg
     4501 atttaacttc acttatgaaa tacagactac cggtaattac taatgtatca ccttgataat
     4561 tttttataaa ttcaacgtag tcatctacta attgtatttc atcattttca atactaccta
     4621 caatttcttc tttgcgtaac gctttcggaa aatcaaattc agttgcataa aacgtatgcg
     4681 caattaaact taaatgtttg accatctcgt taatcggttt tccgtttatt gctgagaaca
     4741 aaatatctac tttttcttta tcatggtact gtttaattgt atcaattaga gcatctatac
     4801 tctctgaatt atgtgcgcca tccaaaatga ttaaaggttt gtcatgcacc tgctcaatac
     4861 gtccagtcca acgaactgat tcaataccgt ctatcatctt attgaaatct aattcaatta
     4921 atccttgttc atttaattca ataagagctg ttatggctaa tgcagcattt tgtttctgat
     4981 gttcacctaa catgcttaaa atgattgttt ctaattcata atctttataa cggtaagtaa
     5041 attcatcatt ttgcgataca acaacaattt ctctatctaa ttcaattggc tttgcatgtt
     5101 gttcaattgc gcgttcacga acatatttta atgcatcttc atttttaaca gcatatatca
     5161 ctggaacgtt aggctttata atcgcgcctt tatccctagc aatatctaga taagtaccac
     5221 ctaaaatatc tgtatggtct agaccgatac tagttaagat tgataaaacc ggtgtaaaga
     5281 catttgtcga atcgttcttt atacccaatc cagcctcaac aatgacaaaa tcaacaggat
     5341 gtatttcacc aaaatataaa aacatcatcg ctgtgattat ttcgaattca gttgcaacac
     5401 ctaaatctgt ttcacgttcc atcatttcac ttactggttt aatacgtgat actaattcta
     5461 caatagcgtc atttgatatt ggcacaccat ttagactaat tcgttcatta aatgtttcaa
     5521 taaacggcga cgtaaatgta cctacttcat aaccattttc aactaaagct gttctaaggt
     5581 aagcaactgt agagccttta ccatttgtgc cacctacatg aataccctta atgttatttt
     5641 gaggattatt aaattgtgct agcatccatt ccatacgttt aacacctggt ttgatgccaa
     5701 atttagttct ttcgtgtatc caatacaagc tctctaggta attcattgtt actaactcct
     5761 atgcttttaa ttgttcaatt cttgccttca caccatcata tttttcttga taatcttgtt
     5821 ttttacgttt ttcttcattt ataacctttt caggtgcttt acttacaaag ttttcattag
     5881 agagcttttt atctactcta tctaattcgc tttgaagttt agctaattct ttttccaaac
     5941 ggctgatttc cttatccata tcaattagcc cttctaatgg taataccact ttacctgcaa
     6001 ttacaactga tgtcattgct ttctcaggaa tttccacgtc agtgctaata tttaaggtac
     6061 taggattaca gaatttgatt aaataatctt tgttttgtga taaagttgtt tcaatttctt
     6121 tatctttagc ttgaattaaa ataggtattt ctttagacaa tggcgtattt acttctacac
     6181 gtgattgtct tacagattta atgatttcaa caagttgttg cattgtttgt ttactttctt
     6241 caaaaatcaa tgattcacgc acttctggcc atgaagcttt aacaattgtg tcaccttcat
     6301 gtggtaaact ttgccatatt ttctctgtta caaatggcat gaatggatgt agcattctca
     6361 taatattgtc taaagtataa ctcaatactg aacgtgtaac ttgtttttgt tcttcatcat
     6421 tactattcat tggaatttta ctcatttcaa tgtaccaatc acagaaatca tcccaaatga
     6481 aattatataa tgcacgtcca acttcgccga attcatattt gtcacttaaa tcagtaactg
     6541 ttgcaatcgt ttcatttaaa cgtgttagaa tccatttatc tgctaatgat aagttaccac
     6601 ttaaatcgat atcttcaact ttaaagtctt caccgatatt cattaaactg aaacgtgccc
     6661 cattccagat tttattgata aagttccaca ctgactcaac tttttcagtt gagtatctta
     6721 aatcatgtcc tggagatgaa cctgttgcta agaagtaacg caagctatca gcaccgtatt
     6781 cgtcaataac atccattgga tccacaccat tacctaatga tttactcatc ttacgcccgt
     6841 cttcagcacg aactaaaccg tgtaataata catcattaaa tggacgacga tctgtaaatt
     6901 ctaagccttg gaatatcatg cgtgctaccc agaaaaagat aatatcgtaa cctgtaacta
     6961 aggcatttgt tgggtagtat cgtttaaagt cttcactttc taaatcaggc caacctaacg
     7021 tagagaaagg ccataaagca cttgagaacc acgtatctaa tacatcttca tcttgttgcc
     7081 aattttcaat atcagttggc gcttcttctc caacatatat ttcgcctgtt tctttatgat
     7141 accaagccgg aatttgatga ccccaccata attgtcttga aatcgtccaa tctctaatat
     7201 tttccatcca ttggttaaat gtatgttcga aacgttgcgg ataaaaatca atacgatcat
     7261 ctgttttttg gttatctaat gaacgtttcg ctaagtcttc catgcgcaca aaccattgtg
     7321 ttgataaata tggttcaaca acagcgccag atcgttctga atgacctaca gaatgaacat
     7381 gatcttcaat cttgataact aaatcttgtt cttttaaatc tttaactagc tgtttacgac
     7441 aatcaaaacg gtccatacct tcatatttac ccgctttgtc gttcatttta ccattttcat
     7501 ccataacgat aatattttct aattgatgtc tttgaccaat ttcaaaatca ttagggtcat
     7561 gtgctggtgt cactttcata gcaccagaac cgaagtctat atcaacatac tcatctgcta
     7621 aaataggcag ttcgcgtcct acgattggta atataacagt tttaccgatt acatctttgt
     7681 atcgttcgtc attagggtta acaacaatcg ctgtatcacc taacatcgtt tctggtcttg
     7741 ttgttgcaat ttcaataaaa ccttcaccat cagcgtaagg atatttaaaa tgataaaacg
     7801 caccttgaac atcttcatgt attacttcaa tatcagataa agctgtacgt gctttaggat
     7861 cccaatttat aatacgttcg ccacgataaa taattccttt attgtataaa tcaacaaaaa
     7921 cttttttaac tgctttactt aaaccttcat ctaaagtaaa acgttctcta ctataatcta
     7981 aacctagacc taatttagcc cattgcgcac gaataaatga cgcatactct tctttccaat
     8041 cccatgcctg ttctaaaaac ttttcacgac caagatcata tctagttatt ccttgttcat
     8101 ttaatttagc ttctaccttt gcctgtgtcg caataccagc atgatccata cctggtaagt
     8161 ataacgtatc gtatccttgc atacgtttca tacgtgtaat gatatcttgt aaagtcgtat
     8221 cccatgcatg tcctaaatgt aatttaccag ttacatttgg tggcgggata acaattgtat
     8281 atgtttcttt tgatttatct tctgacggtt taaaataacc attctttacc cattcttcat
     8341 aacgtcccgc ttcaacttca cgaggatcat attttggttt catttccatt tgctatacct
     8401 cctaaaaaaa taaaaatatc catcctatat acaaatagga cggatattcc gtggtaccac
     8461 ctatattcaa gaaggatgat taatatcaaa ttcactcttt taacataatt ggaataatca
     8521 taccaatact atcatcgtga aatttgaaat gcttcatctc ttcaagcact ctagattatg
     8581 attaacgctc aaacacgtct tagcctacta ttaatcacgt tcagctaaga tactctgtgg
     8641 gctaccttca gtaaaaatca tttacatact cacaccaaat catatgctct ctttaaaata
     8701 atttgaactt actcttccca aatcctatat taaactctta acttatagta taatgattga
     8761 caaaataagt caatgtatag gtgggaataa aatgaatgaa tgcgcatttg gtactaaaga
     8821 tccagtctac ttagactatc atgatcatgt atggggacaa ccgctctatg atagcaaggc
     8881 attgtttaaa cttttagcat tagaatcaca acatgctggg ctatcttggt taactatttt
     8941 aaaaaagaaa gaagcctatg aagaagcatt ttatgatttc gaaccagaaa aggtagcaca
     9001 aatgaccgct caagatatcg accgcttaat gacttttcca aatatcgttc atcatcgtaa
     9061 aaaattagaa gcaattgtta atcaagctca agggtattta aaaattgaac aagcatatgg
     9121 tagttttagt aaatttttat ggtcatatgt aaatggtaag cctaaagatt tgcagtatga
     9181 acatgcttct gatcgtatca cagttgatga tactgcaaca caactatcta aagatttaaa
     9241 acaatacggg tttaaatttt taggtccagt aacagtattt tcgtttttag aagcagccgg
     9301 tttatatgat gcacatttac aagattgtcc atcaaagcct aaacataatt aatttgctat
     9361 agcatcataa agtaaacgag acatcatcta tttttaacta atagactgtc tcgtttttta
     9421 atgtgaaaat ttattcaaat cataagttaa agttccaagt taatgctctg ctaatattag
     9481 atatcttaat ctaaaaatag atgtacaacc tttgcttaaa ccttaataat gaattctatc
     9541 ctaagcataa ttcaacttag atggatgata gtttctaatc ttcacgctta ttcgaacgat
     9601 attttaaaaa gtaattaatt cctggtgcaa taataaatag gatgaaaaag attcggaaaa
     9661 tatgataact cgtaatcata gcaacatcgg caccagtagc taatgcaact aaaactatct
     9721 gattaacccc tcctggtgct gcaccaagaa acaattcatt aataggatta ttatcaaaga
     9781 aatgtatgat ataaaccatg attagcgcac caattatcaa cataatattt tgaattgtaa
     9841 ttgcgattgc tagtctacct tttaaatctg acaataaatg cgcaatttga actccaattc
     9901 taatcatata tattagttgt gccatgttca acaaccaatg atctagtgta aatgttaaac
     9961 ctgtagaaaa attccaaaca attaatacaa tgagtggtgc taataattga aatgttggaa
    10021 actttatttt agacataatt agataaacta taaagatagc tatcgctaaa ataactattt
    10081 gccctatgtt taatacttgt gataaaggca agacttttgt taactttcca ttcgcatgca
    10141 tgttaccatc atgaaaaaaa tatgaaatga acggtactaa aacaacaaca aatataattc
    10201 gtgatgtttg cgttaagcta acaactaaca aattagcacg tttgtcttgt tcagccatga
    10261 ccagcatttg tgttagtgct cctggtataa cacttaaaat agctgtttct gtattaatac
    10321 gtgcaatttt tttaaaaaca aatgccatta ctattgcaat taataatatc gaaatagata
    10381 caacaataat cgaaagccaa ttgtttttaa tatccataac gacatttttc gtaaacgttg
    10441 atccgatttg cacacctaat agtacaatac ctaattcact aagtaagaat ggccatttaa
    10501 tatcaagttt gaaaactttt acacaaatga ttgatgcgat aataggacca aacataaatg
    10561 gaagtaatac gtgcgatgaa tacagtagaa tactaataaa aaatgataaa acgaacacaa
    10621 tgaaattatt tctatatatc attgccatgt tttccacttc tttcaataaa aaataaaatg
    10681 actaaattgc tgcttgagct tcacgtttgt taagataaca atatccgcta gcagttttga
    10741 ctacaaagca tatatggact ttcactatca agtcgccgcc catgccttat atacatttaa
    10801 aaagagcctg aacaaagttc aggctctcaa tttgtccgta tatttatttt acaatacgac
    10861 ttaaagccgt atcaaatgct tgaatcgttt tttcaatatc ttctttcgtg tgtgccgtag
    10921 ataagaatgt accttcaaat tgagatggtg gtaaaaacac accttctttt gccatttctc
    10981 gatacatttc tgcaaataat ttcaaatcac ttttattcgc ttgttcaaaa ttagttacag
    11041 gtccttcatt taagaaataa ccaatcattg aacctgctct atttacagtt attggtacat
    11101 tgtgtttagc aaatacacgt tttaaaccgt cttcaagtat atcgcctaac atattaaaat
    11161 actcatatgt ctctggcgtt aattggctta acgtttcata accacttgtc attgcaagag
    11221 gatttcctga taacgtaccc gcttgataaa tatttcctaa tggtgctata tgatccatga
    11281 tttctttttt accaccaaat gcacctacag gtagtcctcc accgataact tttcctaagc
    11341 aagttaaatc tggtgtcaca ccaaagtaac cttgtgcaca atgataaccg actctgaaac
    11401 cagtcattac ttcatcgaaa attagcaatg cgccgtattc agtcgtaata tctcttaatc
    11461 cctgtaaaaa accttcaatc ggcggtacga cacccatatt accagcaaca ggttctacga
    11521 ttacaccagc aatatcgttt ccaaattttt cgaaagcgat tttaagtgca tctaaatcat
    11581 tgtatggaac tgtaattgta tttttagcaa taccttcagg cacaccagga gaatccggca
    11641 atcctaatgt tgccacccca gaaccagctt tgattaataa cgaatcacta tgaccatgat
    11701 agcaaccttc aaatttcaca attttatttc tgccagtata accacgtgct aatcttaaag
    11761 tatccaatgt agcttctgta ccagatgaca ccatacgcac tttttctatt gaaggtactc
    11821 ggtcaataac gagctgcgcc aatttatttt caagtaatgt tgatgcacca aaacttgtac
    11881 ctttatcaat tgcttcatgt aaatgactaa taacttgagg gtctctatgt cctaaaataa
    11941 gtggtcccca acttagtaca tagtcgatat actcgttacc atcgatatca taaatttttg
    12001 aacctttacc gtgatccata aaaattgctg gtgtatctac tgatttaaat gcgcgtactg
    12061 gactatttac accaccaggc attaaagttt cagcaacctt cattgcttct tctgatttcg
    12121 tatatctcat aaagtaaatt cctcctacag tttatggaaa atcataaata taaaacctta
    12181 tttatctaaa tagcgacaaa tgtcctttgc aaaatacgta ataatcatat cagcacctgc
    12241 acgtttcatt gaaaccattt gttccataac gacacgttct tcatctatcc aaccattttg
    12301 tgccgctgct ttagtcatac tatattctcc actcacatta tatgcaacaa ctggaacatt
    12361 cgtatgattt ttaacatctc gaactatatc taaataactt agagcaggtt taacaatcat
    12421 catgtcgcac ccttctttaa gatcactttc taattcacga agtgcttcca aacggttagc
    12481 agggtccatc tgatacgttt ttctatcccc aaatgatggc gctgaatctg ctgcatctct
    12541 aaaaggtcca aagaaacttg atgcatactt gacaccataa ctcattatag gaatattgta
    12601 atagccggct tcatctaatc cacgacgaat ttcagcaaca aaaccatcca tcatattact
    12661 tggcgcaata atatcagcac cagcttccac ttgagaaatt gctgttttaa caagtagtgg
    12721 caatgattta tcattgtcaa cgtcatgtgt atggtcatca atcacgccac aatgaccatg
    12781 atcagtatat tcacataaac aagtgtctgc aacaattaat aagtcatcat acattttttt
    12841 agcaatacgt gttgcctgtt gaataacacc atcgtgaatg tatgcaccag tacctatatc
    12901 atcttttgag tttggaacac cgaaaaacat aatggcacgt atgcctaagt cataagcttc
    12961 ttttaattca ctttcaagta aattcaaact gatttggtat acacctggca atgacttaat
    13021 ttcttttttc acatcgtctt tttcaactac aaaaattgga tatattaaat cttcttttct
    13081 tacatgattc tctctaacca tatctctcat tgtcgctgat gatctcaatc ttctatgtct
    13141 atcaaatttc atttttagcc cctactttct aaaatctttt caattagtga ttcgagtgtt
    13201 tgaatttctg caattgttac tggttgttga tatgatttaa tggtccgtgc tgtttgttct
    13261 ccaatagcaa aatacgactt gaattttggt acaaatcctt cattaaaata ataacgtact
    13321 gccgacgaac ttgaaaatgt taatgcatcg atttgttgat gttctatcat ttctttaaca
    13381 tcttgtatat tttgtttgtt aggcactgaa gtatataaat ctattttaac aacttcatta
    13441 tctttagata acgctgctaa taacaatggt ctcgccaatt cactcgaagg caaaagtatt
    13501 ttttggttag tttgattaaa tgattttaaa aatccttctt gagaaaagtc gtttggcata
    13561 aaatcaactc gaatgccaag tgattcacaa tattgcgctg tcttacttcc tatcacagca
    13621 atgttatcaa cattaattcc ttttagatat ttataaaaga atttcacagc atttttagat
    13681 gaaaaaataa gccagtcata gcgttgattt aacaaatgaa tatcaaaatt tagtggcttt
    13741 atatcaataa agggtttgtg aataattgat actaaatcac tttgcatgtc atttgtttgt
    13801 gtcataacta caactggctt catatttaaa cgtcactcca ttatttaatg ttgttcattt
    13861 aagcgtttta taatttcata agcaccttgc tcttttaatt tgttactcac tgttttgcct
    13921 aactcaaccg gatctgttcc gttcattgta tattcaaatc gttctttacc atctggggtc
    13981 ataattaaac ctgtaaattc gatttcgttt tgatctgaga ttgtagcata tcctgcaatt
    14041 ggcacctgac aactaccatc catttctgct aaaaacgttc gttcagcagt cacacatttt
    14101 gcaacctcat cattatgtac tttgcttaat aatgttaata gttcttcatc gtcactacga
    14161 cattctatcc ctaaagcacc ttgtccgatt gcaggtaaca atgtatctct atcaagataa
    14221 gatgttacaa tatcatctga ccagcccatt cttcttaaac cagctgcagc taaaataatc
    14281 gcatcataat cttcagtttg taacttttct aatcgtgtat ctatattacc tctaatccat
    14341 ttaatctcta aattaggata cttagataat atttgtgcac cacgacgtaa tgaactagta
    14401 ccaataatac tgccttctgg caattgggat agtggtgtat gtgttttaga aatatacgca
    14461 tcaaaaggta attctctatc agggatacaa cctaatgtta aaccttccgg aattacactt
    14521 ggtacgtctt taagcgagtg tattgccata tcgatatttt tttcaaaaag ttcatgttgt
    14581 atttctttaa caaataagcc tttgcctccg actttagaca attgtttatc tactatacga
    14641 tcgcctttcg tgacaatttc tttaatttca atttctagat ttggctcgac agcttttaat
    14701 ttatcaataa attgctggct ttgtgttaaa gctaatttac ttcttctgga gccaacgact
    14761 aatttacgca tgttcaattc ctcctaggaa cggattgctc tagattattt tctcaattca
    14821 caaaatgtgt tgcaaaaaat aaattaatca tatttaagca aaataaaata atgttatagt
    14881 atattaaata tcttgaattc aaccatttgt tgattctaag taaaatataa cttccatata
    14941 atactgtaat aattgaagag agtattacct tcgggtcaat gaatatacgt tcaccaactg
    15001 aaattacacc ccactgtgta cctaaaataa tactaaatat gagaattatc cacccactta
    15061 acgttgagta aaacacaatt gattcaagtg tagcaacgct accaattcta aagtattttt
    15121 gatcaaaacg tttttccttc aaattacggt attgcatgat atacagtaat gcattgacaa
    15181 aagctaaggc aaagaagaca taacttaaca cagctagacc gatatggact aacagtaact
    15241 cgtctacaac agcaattttc tgaaccttat tagtataatg tgtcggttga aatgtattca
    15301 tccctaaaag tgttaaccct attaaattca agaaaaacac agagaaattc aaaactttga
    15361 taaggttaag aattaaagag attgaaataa tcagccaact taaagtatag aatacatctg
    15421 aaatagaccc taatggaata tgtctagttt gtataataaa aatagataaa gagattgttt
    15481 gtaaaaccca aacaatcccc aataaatata tgcctaaact tctaatctta tgactttttt
    15541 gtacaaaat
//
LOCUS       DAKTLH010000056        15509 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.056, whole genome shotgun sequence.
ACCESSION   DAKTLH010000056 DAKTLH010000000
VERSION     DAKTLH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15509
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.056"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            297..746
                     /locus_tag="O0394_001433"
     CDS             297..746
                     /locus_tag="O0394_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HCY0499517.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGVFTDKYGVRWMLHGQDYTAIQQ"
     gene            complement(827..1414)
                     /locus_tag="O0394_001434"
     CDS             complement(827..1414)
                     /locus_tag="O0394_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0499518.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSFI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(1392..1490)
                     /locus_tag="O0394_001435"
     CDS             complement(1392..1490)
                     /locus_tag="O0394_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499519.1"
                     /translation="MPIINVGDLWDNEHNVYNHWGGLDINDYRSTY"
     gene            1547..3229
                     /locus_tag="O0394_001436"
     CDS             1547..3229
                     /locus_tag="O0394_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HCY0499520.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGTIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWT
                     QGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKQQD
                     VSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN"
     gene            complement(3358..3741)
                     /locus_tag="O0394_001437"
     CDS             complement(3358..3741)
                     /locus_tag="O0394_001437"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCY0499521.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(3743..4594)
                     /locus_tag="O0394_001438"
     CDS             complement(3743..4594)
                     /locus_tag="O0394_001438"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCY0499522.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITIVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            complement(4587..5405)
                     /gene="panB"
                     /locus_tag="O0394_001439"
     CDS             complement(4587..5405)
                     /gene="panB"
                     /locus_tag="O0394_001439"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCY0499523.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            5478..6338
                     /locus_tag="O0394_001440"
     CDS             5478..6338
                     /locus_tag="O0394_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0499524.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HISFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIAHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            complement(6424..7128)
                     /gene="budA"
                     /locus_tag="O0394_001441"
     CDS             complement(6424..7128)
                     /gene="budA"
                     /locus_tag="O0394_001441"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCY0499525.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(7406..8365)
                     /locus_tag="O0394_001442"
     CDS             complement(7406..8365)
                     /locus_tag="O0394_001442"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCY0499526.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
     gene            9052..10500
                     /locus_tag="O0394_001443"
     CDS             9052..10500
                     /locus_tag="O0394_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0499527.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAITFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
     gene            complement(10581..11918)
                     /locus_tag="O0394_001444"
     CDS             complement(10581..11918)
                     /locus_tag="O0394_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HCY0499528.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEIAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            12176..12592
                     /locus_tag="O0394_001445"
     CDS             12176..12592
                     /locus_tag="O0394_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499529.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPYILEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            12715..13605
                     /locus_tag="O0394_001446"
     CDS             12715..13605
                     /locus_tag="O0394_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HCY0499530.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVESKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDNLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNAELIASFSRALASDLRADQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(13798..15294)
                     /gene="mqo"
                     /locus_tag="O0394_001447"
     CDS             complement(13798..15294)
                     /gene="mqo"
                     /locus_tag="O0394_001447"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCY0499531.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
BASE COUNT         4867 a         2862 c         2491 g         5289 t
ORIGIN      
        1 tcagtcaaaa aagttagaaa ttcattttaa tgcatttata tttcgaaaat cccttatgta
       61 tctaaaagca ttttttaaac ttgaatttta aaacactaaa caacacatac gtctctgtgt
      121 cattttcatt ttttgtatgt catatatatg tttacttcat ttaaatcaat ttcatcttat
      181 aatttatcgt gtattttaca aaagattgac ttcaattcat cgtaaaagtt atacttttgc
      241 cattttttaa tgtaacatgg tgttagtaat aaaaataata cattgaggtg ttttacatga
      301 cagcattatt cccttatatc gcttttgaaa attcaaaaga agcccttgca tattacgaag
      361 aagtatttgg tgcaactgac gttaaacgtt tagaagttgg cgaagaacaa gcgtcacatt
      421 ttggtatgac taaggaagaa gcgcaagaag caactatgca tgctgaattt gaagtgcttg
      481 gcgtaaaagt gttatgttct gattcttttg gtcgcgctga caaaattaat aatggcatat
      541 cattattaat tgattatgat gttaacaata aggaagatgc tgataaagtt gaagcattct
      601 atgagcaaat taaagatcat tcttcaattg aaatagaatt accgtttgct gaccaattct
      661 ggggtggcaa aatgggcgtc tttaccgata aatacggtgt tcgttggatg ttacatggtc
      721 aagactatac tgcaatccaa caataatgtt attgataaag atatcattta ataagcttat
      781 gttatttagc aaggcaagcc actctcgatt catggcttgc cttattttat tgtatatttt
      841 taattatcgt tgctggccct tgattgacaa tcgtattaaa atgccttatt aagtcaactt
      901 tgtctatacg gtttggatct tctacccaat gtcttataaa agacaatccc gcacctgaaa
      961 cataactcat gaaataagaa aatggtatac cattaatttg atcattttca tttttataac
     1021 gttgtaaatg acacataatt aaatcataaa gtttttcgtg gatatttgaa tctttaccca
     1081 tacgaaacat caattgataa aatgcgatgt ctttttctat catttctatt aaaacggtca
     1141 taatttgatg tatgttatcc gtggataact taactgctcc atttaacttc tcatcatgaa
     1201 tgaagtctct tatttcctcc aactgctggt cctctaattt ttcaagcaaa tcatacttat
     1261 cataataatg cgtataaaat gtactacggt taacatcagc taaatctgca atttgttgca
     1321 cagtaatctc ttctaattgg tgttgatgta aaagttcaat aaatgcattt ctcattgcaa
     1381 cttgtgattt tctaatacgt cgatctatag tcatttatat caagtcctcc ccaatgatta
     1441 taaacgttat gttcattatc ccacaaatct ccaacattga tgattggcac acaatgttta
     1501 cctgtttaat ataggtgata caaacaaaca gaaaaaggtg ataacaatga accaacattt
     1561 actaggaaat ccaaaattaa ctgtaactca tgtcaatgaa gttaaagccg gtattaacca
     1621 catcgttgtc gacagtgttc aatatggaaa tcaagaaatg attatggaaa aagatgtcac
     1681 tgtggaaatg cgcgatggcg aaaaattata tattaatatt ttcagaccaa ataaagatgg
     1741 caaattccct gtagttatgt ctgcagatac ttacggtaaa gataataagc ctaaaatcac
     1801 aaatatgggt gccctttggc caacattagg taccattccg acatctagtt ttacacctga
     1861 agaatcacca gacccaggat tttgggtgcc aaatgattat gttgtagtta aagttgcatt
     1921 acgcggtagt gacaaatcca aaggcgtctt atctccatgg tcaaaaagag aagcggaaga
     1981 ttattacgaa gtgattgaat gggcagcaaa tcagtcatgg agtaatggaa atatcgggac
     2041 aaatggtgtt tcttatcttg cggtgactca atggtgggtc gcatcattaa atccaccaca
     2101 tttaaaagca atgattcctt gggaaggctt aaatgatatg tatagagaag tagcctttca
     2161 cggaggtata ccagatactg gcttttatcg tttctggact caaggtattt ttgcgagatg
     2221 gacagataat ccaaatatcg aagatttgat tcaagcacaa caagaacatc ctctgttcga
     2281 tgatttttgg aaacagcgtc aagtgccatt atcacaaatt aaaacacctc tactaacatg
     2341 tgctagttgg tctacacaag gtttgcacaa ccgtggctct tttgaaggat ttaaacaagc
     2401 tgcatctgaa gaaaaatggc tatatgtgca tggacgtaaa gagtgggaaa gttactacgc
     2461 tagagaaaat ctcgaacgcc aaaaatcatt ctttgatttt taccttaaag aagaaaataa
     2521 cgattggaaa gatacgcctc atgtcattta tgaagttaga gatcaatttt ataaaggcga
     2581 attcaaatca gcgtcagcgt tccctttacc taacgcagaa tatacaccat tgtatttgaa
     2641 tgctgaaaat cacacattga atcatgcaaa gattagtagc gcgcatgtcg cacaatatga
     2701 ctctgaagat aaacaacaag atgtaagttt taaatatacg tttgacaaag atactgagtt
     2761 agttggaaac atgaacttaa aactatgggt aagcactaaa gactcagatg atatggattt
     2821 atttgcaggt attaaaaagt tagatcgtcg tggtaatgaa gttaacttcc ctgattttaa
     2881 tcatattgaa aatggtcaag tagctactgg ttggttacgc gtatcacatc gtgaattaga
     2941 tcaagaaaaa tcctcaatcg cgcaaccttg gcataaacat gaaacagaat taaagttgtc
     3001 acaagatgag attgtacctg ttgaaatcga attgttacct tcaggcacgc tatttaaaca
     3061 aggcgaaaca ttggaagttg ttgtaaaggg tagtgaaatt gtaattggta atagtactcc
     3121 tggcatgaaa acacgttatg aacatgaaga aaccgtaaat aaaggcatgc acatgattta
     3181 tactggtggt aaatatgatt cacaattaat cattcctatc gttaattgat atgcagcaat
     3241 tacggtcgct tttgattaaa agtgacatag tgataggact gtataacaag agaaagccac
     3301 acgcttggaa tcttaaacca aggtgtggcc ctttttatta ttgatggcta tttaatttta
     3361 taacactatc gtattttctt tttcatgaat catttcaata atgacattat cttcattcat
     3421 tactgctact ttaggtgcat ggtttttaat ttcttcttca ttcaactgtg cataagtcat
     3481 gattatgact acatcgccta cttcaacaag tcttgacgct gcaccgttta aacaaatttt
     3541 accactacct ctttcaccag ctattacgta tgtttcaaaa cgtgcaccat tattattatt
     3601 cacgatggct actttttcat ttggcaagat gtctaccgct tccaatatat ctgaatcaat
     3661 cgtaatgcta cctacataat ttaaatttga ctcagtcact cttgctctat gaattttagc
     3721 attcatcatt gttcttatca ctttattcag ctccaattat tatattatct attaaacgcg
     3781 cttttgaaaa tttaacagct aacgagataa atatgcgtcc agttatttcg tgttgttcta
     3841 ctaattgagg ataactataa acagcaactt cttcaatgcg tccacttata tgtgattcaa
     3901 gatattcagt aaccctgtct ataattactt tactttgacg ttcaccgtct tgatacaacg
     3961 cttgtgctaa tagcaaactt ttacttaaat gtaccgcttc ttgtcgttct tgctccgtta
     4021 aataaacatt tcttgaactt ttcgccaaac catctgcttc tcgaacgata tcaataccaa
     4081 taatttcaac ggcatgattg aagtctttta ccatttgctc gacaatagcc aattgctggg
     4141 catctttttt accaaaataa gcataatccg gcataacaat attaaatagc ttattaacta
     4201 ctgttaccac cccatcaaaa tgccctggac gcttcgctcc ttctaacaca tcagctaatg
     4261 ggcctacttt gacatcaata cctaattcac ctggatacat atcttctact gcaggatgaa
     4321 aaacaatgtc cgctcctact tctgatacta attctaaatc tttatcaatt tgtctcggat
     4381 aagcatcgaa atcttcgttt ggaccaaatt gtaatggatt aacaaataca ctcacaattg
     4441 taatatcatt tgtactaact gattcgcgta ccatcgttaa atgtccatca tgtaaggcac
     4501 ccattgttgg gataaaacca atcgttgtgc ctgagcgttt ggctgcttta acaatgtgtt
     4561 gcatctcttt taccgtagta atcagcttag tcattgttat taacctcatt cataattttc
     4621 tttttatacg tatattcttc tgatggaaat gcaccagatt taacttcttg atcgtattgt
     4681 tttaaaccat ccacaccaac actaaaatca gcaaattgct tcacaaattt cgctttatgt
     4741 tcaacaccat aatttaacat atcgtgataa accaatactt gaccatctgt accttttcct
     4801 gcaccaatac caatgactgg aattgttaag tgcttgctaa tttcttctgc taaatcattt
     4861 ggaattgctt caagtactaa cgcaactgca ccagcttgtt ctacattttt cgcgtctaaa
     4921 ataagttgct ccgctgcttc tttcgttgca ccttgtaatt tataccccat aacgccaaca
     4981 ctttgaggtg ttaatcctaa atgtgcaaca acaggaatac caattgccgt tgctttttca
     5041 ataaatggtg taatatgcgc tccttctgct ttaattgcat ttgcattcgt ctcctgataa
     5101 agctttagag catgatttaa gtcttgtgtc atagagatgc ctactgcacc aatcggcata
     5161 tcaacaacta caaatgtatt tggtgcgcct cttcttactg cacgaccgtg atgaatcata
     5221 tctgctaacg tcacttgtac ggtactttca taacctaata cagtcatacc aagtgaatcc
     5281 ccaacaagaa tcatatcaat acccgctgct tccacttgtt tagcacttgg aaaatcataa
     5341 gctgttacca tagaaatttt agtttgcttt tgtttcatat ctattaattg acttactgtt
     5401 ttcaatgtta ttcaacctct ttttgcagta ttattagaaa tagtataatg aatcttttat
     5461 agaaaggaaa gaagaagatg ttatcagttg cgattatcgg cccaggcgct gtcggtacaa
     5521 ctattgccta tgaattacaa caatcattgc ctcatacaac gcttatcggg agacacgcca
     5581 aaacaataac atattatact gtaccacatg cacctgcaca agatattgtt gtgaaaggtt
     5641 atgaagatgt cacaaacaca tttgatgtta ttattattgc agtcaaaaca catcaacttg
     5701 atgcagttat tcctcattta actcatcttg cacatgagga cacgctcatc attttagccc
     5761 aaaatggtta tggtcaactc gaacatattt catttaaaaa cgtatgccaa gcagttgtct
     5821 atataagtgg tcaaaagaaa ggcgatgttg ttacgcactt cagagattat caattacgta
     5881 tacaagataa tgcattaact cgacaattta gagatttggt ccaagacagt caaatcgaca
     5941 ttgtattaga agcaaatatt caacaagcta tttggtataa attgttagtt aacttgggca
     6001 ttaattctat cacagcactc ggtagacaga ccgttgcaat catgcataat cctgaaatac
     6061 gtacactgtg tagacagcta ttactagatg gttgtcgtgt tgcacaggca gaggggttaa
     6121 acttttcaga acaaactgtc gatactatta tgactatata tcaaggttat cccgacgaaa
     6181 tgggaacgag catgtattac gacattgcgc atcagcaacc tcttgaagtc gaagcgattc
     6241 aaggctttat ttatcgacgt gcacgtgaac acaatcttga caccccatat cttgatacta
     6301 tttatagctt tttacgcgcg tatcaacaaa atatgtaagc acatatataa tataaaaaga
     6361 ccttaacgat gcatttagaa ccacctgttc ctaatatcgc taaggtcttt taattatatt
     6421 agactattca gcttctctaa tttcttctgc cacatcttta tagtctattt tcgctttaac
     6481 aaacgtctcg ttatttaccg ggaaatgttg ttggaatgtt tcaaagtttt gtatctcaac
     6541 gacaacgtca tccacttcaa agtcaagaac atgtccacca taagctcttt catcatccgc
     6601 aaaatgtata tgaaaaccag cagaccctac gccatgaaat aattctggtg taaaaaatcc
     6661 aacaatggca ccacgaatat cttgtctttt ttcctcaggt tgtctgcgtg ctgaatcaat
     6721 caaacgtgta tatggcggtt gctgagcagg catcattcgt acatgcatat gtttaaatgt
     6781 gccataaatt ttaacagccg aaaataaatt ctcacttaac atttcatttt taatttgtgc
     6841 aaatacatca tcttgtgata attgttgcaa tggaaatgtc ttactcgctt taaaattagt
     6901 aatcgatgca tacggtactt tctcatcgcc ttttaattct ataaattctt tatgctcgtt
     6961 agcatgatat gcctttccgt ctaaaaatat tacttcgcca tcagaccctg ttaacgttgc
     7021 aatccctaaa ttcccatgtt ctaacaattc attaattgta gctgtgcctt ctagtaagcc
     7081 agccattaac gtacctaatg taccatgttg atacaagaca ttcgtcattc caaaaccatc
     7141 tccctatagt tattcctcgt tgtcatgctc tacttcgtga ttaatgcaga actactttcc
     7201 tttatactca atgacgcctc aacattttta ttcatgtcac taaattttca cgagtcataa
     7261 tactttattc aattattatt ttacgcaaaa gtattgcctc tatcaatgct tgctaaaaaa
     7321 atggacctat cagtgccacg tgttactgca acactaatag atcctcttac cattaaaata
     7381 tttctaatta atacttaaaa taggcttagt cttctaataa atatttaatt gaatcaaatg
     7441 tatcttctaa tgttttaaca gatttatcga acaacgcttg ttcttcggca cttaatggca
     7501 tttcataaat tttaactgcg ccatgttggt taactaatgt tggtacacct aggtaaacgc
     7561 ctttgtgacc accatattga ccatctaatt gtatagaaac atttaagaca ttattttcat
     7621 tatttaaaat ggcttttgaa atgcgcatca atgctaatgc aataccatag tatgttgacc
     7681 ctttagcttg gataatttca taagcagcgt cacgtgtatt cacataaatt tcttccgctt
     7741 tagcttcgct accagtttgt tcttttaatg tgtcatatac tgaaatacct gctacatttg
     7801 cttgtgacca aactgcaagt tcagtatcac catgctcgcc aataatacta gcgtcaacac
     7861 ttgaaggtgc aacaccaagt tcttggctaa ttaaatattg taaacgtgca ctgtccaata
     7921 cagtacctga accgataaca cgctctgctg gtaatccagt atattctttt acaaatcttg
     7981 ttaaaatgtc tacagggttt gccgcgatta agaaatatcc atcaaagcca ctatccataa
     8041 cactcttaac gatgctcttc ataatcttag tatttttttc aactaattgt aaacgtgttt
     8101 cacctggctt ttgaggtgca ccagctgtaa taacaactaa atctgcatct ttacagtctt
     8161 cgtattcacc tgctttcaca tcaactggtg aaggactgtg gactgtacca tggtttaaat
     8221 cttgaacatc tgcttttact ttgtcttttg caatgtcaat aattacaaat tcatcagcaa
     8281 caccttgcgt aaccattgca aaggcatagc ttgatcctac agatccatct ccgattaata
     8341 caaccttttt accaaatgtt ttcataatta tgtctcctaa cctaattgaa tttattttac
     8401 acgtgactac acgtaaaaat atctatactg aaattaaaga ttaacaattt tattttatta
     8461 taatattatg ctaccaaatt acaaacataa aactgagatg taagcggagt caaattacac
     8521 tctatcgcca ataaatattg atatctcaag ctttagcacg taatttgaaa tttgtgacaa
     8581 tttttagcat tctaaaattg cgtaacgacg ctatttaaag gctttaacaa actattgagc
     8641 tttctatttt tttcatttta aaaaggatgg ctattcgact taaatgactt actatataac
     8701 ctcctatttt ccgccttacc ctcatcatat ttgatgcatc attttatagt caaagaatta
     8761 catttcatac aaactagcca atcagacata aacatgaaat tgattttaaa aatataatgt
     8821 gcaaaattgt tagaaaatta gcaaaaaata gtacttattc atttaatatc tgttataatc
     8881 agttttatca tttaaaaaat ctatggatgt tatcaagaca agtcattaat acgtaacata
     8941 aagttgtgat ttttcagaat ttttagacaa aatattgatt atttgtgcta tgatataaca
     9001 tattaaaaaa caccgtatag aattgtttat gaatgtttgg gaggaaagtt tatgggaagt
     9061 tttttcaata aaatagcacg aaaagaggat ccggctatct atcaaaataa agatggtcat
     9121 ttaaagcgta cacttcgggt gcgtgatttc ttagctttag gtgtaggaac aattgtatcg
     9181 acatctatct ttacgctacc tggcattgtt gctgcagaac atgcaggacc ggccgttgcg
     9241 ttatcattct tactcgctgc tattgttgct ggtttagttg catttactta tgcagaaatg
     9301 gctgccgcta tgccatttgc aggttcagcc tattcttggg tcaatgtatt atttggtgaa
     9361 ttttttggat gggttgccgg ttgggctcta ttagctgaat attttatcgc cgtagccttt
     9421 gttgcatcag gattctcagc gaatttacgc ggacttgtga aaccaattgg catcgaatta
     9481 cctgcagcat tatcaaatcc atttggtaca aatggcggtt ttatcgatat tattgctgct
     9541 atcgttattt tattaactgc attattacta tcacgtggta tgtcggaagc agctcgtatg
     9601 gaaaatattt tagttatttt aaaagtatta gctattattt tatttgtcat cgtaggttta
     9661 acagcaataa atgttagtaa ctatgtgcca tttattccag aacacaaagt aactgctaca
     9721 ggtgactttg gtggatggca aggcatatat gctggtgttt caatgatttt cttagcgtat
     9781 atcggtttcg attctatcgc agcaaactca gcagaagcac ttgatcctca aaagacaatg
     9841 cctagaggta ttcttggttc tttaagcgtt gctatcgtat tatttattgc tgtagcactt
     9901 gtgttagttg gtatgttcca ttactcacaa tacgcaaaca atgctgaacc tgttggttgg
     9961 gctttacgtc aaagtggtca tggtgttgta gcagctattg ttcaagctat ctctgttatc
    10021 ggtatgttta cagcattaat tggtatgatg ttagcaggct cacgtttact ttattcattt
    10081 ggacgtgacg gcttattacc ttcatggtta agccacttaa acgacaaaca tttacctaat
    10141 cgcgcactag ttatacttac tattattggt gttttaattg gttctatgtt cccattcgca
    10201 tttttagcac aattaatttc agcaggtaca cttgttgcat ttatgttcgt ttcattagca
    10261 atgtatcgtt tgagaaagcg tgaaggtaaa gatctaccaa ttcctgcatt taaattacct
    10321 ttatatcctg tgttaccagc aattacattt gttttagtat tgctagtatt ctggggatta
    10381 ggattcgaag cgaaattata cactttaatt tggtttattg ttggtatcat cctatattta
    10441 agttatggac ttagacattc taagaaaaat gacgtagctg aataccaccc accaaaataa
    10501 aaaataaaaa aaatcatttc ccatctgatt aacatccaga tgtggaaatg attttttatt
    10561 ttatattaag ataatcgcta ttaccaacct tgtccagata tgtcatattg atctaagtta
    10621 cctgcttcca aagcagtcag tgcatcttct atagtgttta acgccgtgtc taattgctca
    10681 tatgttatta ccaatggcgg ttggaatcgc aacacatttc ctgctacagc tataataact
    10741 acgccatgct caaagcagta attacaaatt ttaagtgccg cactggcatc acgtgttttg
    10801 agttttttgt cggaaacaat atcaatacca atgctcagac ctttacctct aacatcgcct
    10861 acactattgt atttagatac ccattgatcc attcgtttcc taacatattc ccctttttcc
    10921 gcactagcct gaagaagcga ctgatcttca atcatttgaa ttgtggctaa tgcagcttca
    10981 caactaactg gattagcacc tgttgtaaat aaatgtgctg gtgcttctaa acaattcata
    11041 atctctttgc gtccaacaat tgctgacata ggcataccac ctgctaagga ttttccgaaa
    11101 gtgattaaat caggcgtaaa attaaaatgc gagactgaac tccatgtacc tgttctccca
    11161 aaaccttgtt gaatatcatc gacagcgatt aaaataccat gttcacgaca aatcttttct
    11221 aacgcttcaa aataccctgg aactggttct aaaagtccac catcgccttg tatcgtttca
    11281 ataacaatac atgctacttc gtcagcaggt acatacttcg caaacatttc ttttaagggt
    11341 gctaaatatt cttctactga attagcttgt ggctgctcgt acataccacg atatttatct
    11401 ggaaacggaa tatgataaaa accattcaat aacggaccat aatgtttgcg catatttaaa
    11461 ctaatagctg acatagacaa tgagccaaaa gttgaaccat gatatgcatt agtgaaacta
    11521 atgatataag gacgccctgt atatgctctg gcaaatttaa tgatgccatc atttgcgtct
    11581 gatccggtta atccgaaggt cactcttttt tcaaaatctc caggtgcaat ctcacaaagc
    11641 ttcttagcta aacgtaccaa cggttcatga tacatatatg ctggtgtata atgaataaat
    11701 ttatcaactt gtgcctttat cgcttctgtt acttctctag gtgcatgacc tacgttttga
    11761 gaactcgcac tcgataacaa atcgatatat gtcttcccct caatatcaac caatgttgct
    11821 ccatatccat gatcaatcac taacggataa tatttaatac gtcctgattt cgcaaaataa
    11881 tgttcatcct cttgaattaa ttgatgtgct ttactcatat acattcccct cccattgaca
    11941 gtattaatgt gcaagcaaag tggcctctct taaatcgatt ataaaatccg atttaaaatc
    12001 ctgctcaaat accgtcatgc ctctatgaat actatccaat atactaataa aattcagatt
    12061 tgtaaatata ttctgataat taagaatttt atatcaatct actttgttta tgcgcataat
    12121 tttgttcgat tagggaattg tttactatat tccatttgac tctgaggtga tacgaatgcg
    12181 tttaaaagta ttgttccatt tcattgctgc tattttcata tcattcatgt tgctatggat
    12241 gaccatgttg tttgacttaa taagcaatca atcacattta aaagctttat tattgaattt
    12301 agattttcta attccttctg acaatacacc atatattcta gaaataatat gtcacttact
    12361 aattggtagc gtcatttatt ttgtatttgt gttgctgttt catacttcta aaaggcttta
    12421 ctatctatgt tatatccctt tatttttctt atttatagca ttgtatccct ttttagtatt
    12481 tattgcgcaa cgtccaattt ttcaatttag tgtgacagaa ttaatcggtt ggattattac
    12541 acatattttc tttatgtcat taatggcgtt agtcatacct agaataaaat aatattttta
    12601 attaaactga caattatttt tcacatttta tacacttttc aaggtttatg acttgagaaa
    12661 tgttctcatt tttatcatac tggaggtgta ttgaaaagtt aaggagtgga aagtatgaat
    12721 aaagagcaat tagaaaaaat gaaaaatgga aaaggcttta ttgccgcatt agaccaaagt
    12781 ggtggtagta caccaaaagc acttaaagaa tatggtgtaa acgaagatca atatagcaat
    12841 gaagacgaaa tgttccaact tgttcacgat atgcgtacac gtgtggtaac ttcaccttca
    12901 ttctcaccag ataaaatttt gggtgctatt cttttcgaac aaacaatgga tcgcgaagta
    12961 gaaagcaaat acactgcaga ttacttagct gataaaggtg ttgttccgtt cttaaaagta
    13021 gacaaaggtc ttgctgaaga gcaaaatggt gttcaattaa tgaaaccaat cgacaactta
    13081 gacaatttat tagaccgtgc aaacgaacgt cacatttttg gtacaaaaat gcgttctaac
    13141 attttagaat taaatgagca aggtatcaaa gacgttgttg aacaacaatt tgaagttgct
    13201 aaacaaatta ttgctaaagg tttagttcca attatcgaac cagaagttaa tattaatgca
    13261 aaagacaaag ctgaaattga aaaagtatta aaagctgaac ttaaaaaagg tttagatagc
    13321 ttaaatgctg atcaattagt aatgttgaaa ttaacaattc ctactgaacc aaacttatac
    13381 aaagagttag ctgaacatcc taatgttgtt cgtgtcgttg tattatcagg tggttacagc
    13441 agagaaaaag caaatgaatt acttaaagat aacgctgaac ttatcgcaag tttctcacgt
    13501 gcattagcta gtgatttaag agctgatcaa tctaaagaag aattcgataa agctttaggt
    13561 gatgctgtag aatcaatcta cgacgcatct gtaaacaaaa actaatttat acattaaact
    13621 attttttgat ataaactact aaaaaggact catacatgtg ctttaaaggc attgtgtatg
    13681 agtctttttt gattaaagtt gattttgccg tatgtattag attacgtaaa aaaatcggac
    13741 agctgaaaaa tctttaagat ttctcagttg tccgatttta aatatagcta ttagaattta
    13801 gttttcgtag taacctaatt ctaagtcttt tgaagtttgt ttacggattt ttctcattaa
    13861 tttttcgtct tcaattaatg attcaccgta tgatggaatc attttcttaa ttttaggtgc
    13921 ccattcagtt ttgtattcag ggaagttacg ttctaatact tctaacgcaa ctgaaactga
    13981 agttgaagcc cctggtgatt cacctaataa tgcaattaca gtgtggtctt gtgagttaac
    14041 cacttctgta ccgaattgga tgaatccttt accgtgttca ggtgtatctt tgataacttg
    14101 tacacgttta ccagcagtgt ataattgcca atcttcatta cgtgcttctg gatagaaagt
    14161 acgtaagtgg ttcatacaac cttcttttgt cataattact tggtcaaatg agtatttaat
    14221 taaaggtaag tttttaactg ctgctgctaa taaagttgta atgttgtatg ttttaacaga
    14281 cttgaataaa tctaagttag aaccattttt caagaattta ggtccaacat tagcaaatgg
    14341 tccaaataat aatgttcttt gaccatcaat gtaacgcgta tctaaatgag gtacagtcat
    14401 tggtggtgta ccaggtggct ctttaccata aactttggca tcgtgttgtt caataacttg
    14461 tgggtttgta caagctaaga attgaccact gatagggaat ccacccaaat gtttactttc
    14521 agggatacct gttttttgta ataatggaat tgctccaccg ccagcaccga tgaatacgta
    14581 gtcagttact tgtttgaatt tctcaccagt taggcgattt ttaacagtaa cttcccattg
    14641 accatttgat aattgttcaa aatcaacaac ttcatggtta aattgcactg tagcatttgg
    14701 atgtgcttca atgcttttag ccattttacg tgttaattca ccgaagttta catctgtacc
    14761 ttcgtcaatt ttacttgccg ccatgatacc agggttatct tcacggcctt tcatcatcaa
    14821 tggaatccat tttttcatta cttcgatgtc ttcagtatat tcgatattat cgaacatagg
    14881 gaaagctttc atcgcttcgt aacgatcttt taagaattta acattgtttt tacctctaac
    14941 ataactgatg tgtggtaatg gattgataaa ttctcttggg ttctcgatgc taccgctttt
    15001 cactaagtga ccccagaatt gttttgaaat ctcaaactct tcgttaatca ctttcgcttt
    15061 ttcgatgtcg atagaaccat caggttgtaa aactgtgtag ttcaactcac ataatgctgc
    15121 atgacccgta ccagcattat ttctttcgtt tgaactttcg attgcaggac gatccaagcg
    15181 ttcgtaaacg tggatattcc agtctggctc aatttctttt aacattgaac caaatgttgt
    15241 gctaagtaca ccggctccaa ttaaaacgat gtctttacta ttagacttag ccattggttt
    15301 cacctctcca aaaattgtaa atgtgtatca tcaatatgaa agttacataa aactgacata
    15361 tttctttaaa atatcaacgc cattgataac ttcctgtttt aattgatacg ctgtaacaaa
    15421 atactatagt tagtgcttac atgtatatgt taaagcaagc agtggtaaat gtaaattata
    15481 attattcatt aactttgcaa tatattaaa
//
LOCUS       DAKTLH010000057        15279 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.057, whole genome shotgun sequence.
ACCESSION   DAKTLH010000057 DAKTLH010000000
VERSION     DAKTLH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15279
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.057"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            184..699
                     /locus_tag="O0394_001448"
     CDS             184..699
                     /locus_tag="O0394_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HCY0499532.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSVSESKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGIIVTGTINREKYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            complement(848..1819)
                     /locus_tag="O0394_001449"
     CDS             complement(848..1819)
                     /locus_tag="O0394_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HCY0499533.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNLQDIIEDLSTAYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVSPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            2063..3019
                     /locus_tag="O0394_001450"
     CDS             2063..3019
                     /locus_tag="O0394_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCY0499534.1"
                     /translation="MNYQVLLYYKYMTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDD
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETK
                     YLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            3171..3809
                     /gene="pcp"
                     /locus_tag="O0394_001451"
     CDS             3171..3809
                     /gene="pcp"
                     /locus_tag="O0394_001451"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCY0499535.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTFVCNHTLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVIGKPDTPSMPLEKIVAGLTAAIEAISNDEDLHLALGTTE"
     gene            complement(3928..4695)
                     /locus_tag="O0394_001452"
     CDS             complement(3928..4695)
                     /locus_tag="O0394_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499536.1"
                     /translation="MLSKQSQDFLEKLRVELLFRGKTEAEVEELLEELEDHLTMAEKN
                     NEDTSSIINTPIKSMADQLSPEISLTTGLYKYITLYFAFILAVIIIPRFLDLGTFDVT
                     IAFLLYIASIVILGVVIGMLILRNTLVRFGDQKITYVINVTYGIIVFLWMILGGLIIK
                     KYPIYSFFELSDKQSFIVGLVLLIALIGTCILIKQKIYALIVLVISLPSLIARIAAIF
                     SKEPDTFNLVSVSILIILNIAFIIYTFVNFRKLKKED"
     gene            complement(4685..5023)
                     /locus_tag="O0394_001453"
     CDS             complement(4685..5023)
                     /locus_tag="O0394_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCY0499537.1"
                     /translation="MSVSDQIMKGLLDGAILGLIAQGETYGYEIMTKLKNQEFPEISE
                     GSIYPVLMRLNKKGYVDTTIKKSSNGGPRRKYYTITDSGLKELKVFKSKWQVLNKGMI
                     NLFGGENDVK"
     gene            5190..5654
                     /gene="bstA"
                     /locus_tag="O0394_001454"
     CDS             5190..5654
                     /gene="bstA"
                     /locus_tag="O0394_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HCY0499538.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDAWKVEKVLDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIERILEGLQTLPER
                     ARNLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            5892..7310
                     /locus_tag="O0394_001455"
     CDS             5892..7310
                     /locus_tag="O0394_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCY0499539.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVVALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(7723..8631)
                     /gene="rarD"
                     /locus_tag="O0394_001456"
     CDS             complement(7723..8631)
                     /gene="rarD"
                     /locus_tag="O0394_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCY0499540.1"
                     /translation="MNSEYKKGIFLALSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDMNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            8856..9431
                     /locus_tag="O0394_001457"
     CDS             8856..9431
                     /locus_tag="O0394_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0499541.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEAVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVQSSLPFKLTGDVSCNHAPKRQQMIDFIVVTAALDIELLRS
                     LVTTEFIWSVPGRFDIYGPQILIQELSNHYNQVASLNIHSSITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVG"
     gene            9596..10612
                     /locus_tag="O0394_001458"
     CDS             9596..10612
                     /locus_tag="O0394_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HCY0499542.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSR"
     gene            10855..11649
                     /locus_tag="O0394_001459"
     CDS             10855..11649
                     /locus_tag="O0394_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HCY0499543.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPLHAIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTHPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKFDVTKDNYKHFLEKYFGLNVTINRIEKQ"
     gene            complement(11783..12295)
                     /locus_tag="O0394_001460"
     CDS             complement(11783..12295)
                     /locus_tag="O0394_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HCY0499544.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIVYRRKAKALVGGNQNWILFMGIVDILFGLLVIFNVGASSAFFIYMFAFW
                     FIFSSISGLFTFSGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            12578..13336
                     /gene="vraD"
                     /locus_tag="O0394_001461"
     CDS             12578..13336
                     /gene="vraD"
                     /locus_tag="O0394_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HCY0499545.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            13326..15206
                     /gene="vraE"
                     /locus_tag="O0394_001462"
     CDS             13326..15206
                     /gene="vraE"
                     /locus_tag="O0394_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HCY0499546.1"
                     /translation="MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     IIAYVLILFKSALFLKRRSILSMMKDSIKTDATTAKVTTAEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQ
                     DAKQFETKLSQQQITFSKNAYETITVDNVNDQVITLENGSDSGRTNSILSANNKLTGN
                     NAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGKVYPLNLSFNSPVIEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQAIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKK
                     TI"
BASE COUNT         5098 a         2591 c         2454 g         5136 t
ORIGIN      
        1 gtttgaatta attgactagg gtataacaat agtgtggtgt tttataggtc attctttaaa
       61 tacatcacag aaaatattcg aattcgaaat aaaatgcttg catctttgtt ttttaggtgg
      121 tattgtatgt atatcaagta taaaaaatat atctaagaga taaaaatatg gaggttttat
      181 attatgacta actttacttt tgatggtgca cacagtagtt tagaattcca aattaaacat
      241 ttaatggttt ctaaagtgaa aggttcattt gatcaatttg atgtagctgt tgaaggagat
      301 attaatgact tcagtacttt gaaagctact gcaacaatta ttccaagctc aattaacact
      361 aaaaacgaag cacgtgataa ccacttaaaa tctggtgatt tctttggtac tgacgaattt
      421 gataaaatta catttgtgac aaaatcagta tctgaaagca aagttgttgg tgatttaaca
      481 attaaaggca tcactaacga agaaacattc gatgttgaat tcaacggagt aagtaagaat
      541 cctatggatg gttctcaagt aacaggtatt attgttactg gtacaatcaa tagagaaaaa
      601 tatggcatta actttaacca agcacttgaa actggtggcg taatgctagg caaagatgtt
      661 aaattcgaag catcagctga attctcaatc tcagaataat ctcattacac aatcctcatt
      721 gttttaatat atatacgaaa tgccaactat atcatcccta ggtatagttg gcatttttcg
      781 tttaactcat gtgtaacaag cactaactgt tcaccctaat tttagacgcc tttcattcag
      841 gtaggtctta ttgaaactga aaactttgat gaccttttgc aaagccatta actgtataaa
      901 gcatagatcc tccgcccatt tctatatcat tggaacaaat gatgagttga tttgttccag
      961 gtataaattg cggatgagta gaacgtaaca tatgcccttc atctcggcct ggtatcaata
     1021 tttgtcctat tggataaccc cttttattaa aaactaacac tcgaccttga ccatacattg
     1081 ctacgtataa attatcgtca ctatcaatac aacatgagtc tggtccttca tgacctgtaa
     1141 aatagtacgg tatggtagct ccaaatggtt gtatcgtcac accatcatct tcaagtgcaa
     1201 tgcgatgtaa tcgattggct gttgtttctg ttacccatag tactttttca tctgtactta
     1261 aagcaatacc atttgctacg ctaatatttt gaatgatagg cgtcactgtt ctaaagtccg
     1321 gcgaaacata ataaacgcct cctagtggat tggtagagta tcctctaaaa tctgtaaaat
     1381 aaaatccacc tttagaatca aataccatgt catcaataca atatgctgtt gaaagatctt
     1441 caataatatc ttgtaagttg tcaccatttt ctgtagctgc aaaaatgcct cctgtagatt
     1501 taaaatctcc taaataacaa acgaataatc ggccatcttt atgtattttg attgctgcag
     1561 gattcgcttt gtgacttaca aaaggtcgtt tgatttcctt cgtttcagga ttgattttga
     1621 aaatattgcc ttcgaataca tccaataaaa agagttgtcc ctgccgatca aagttcaatc
     1681 cttctagttg caatcctttt ttggaaattt caagccatgg ttcagctgta attgtttgta
     1741 attcactttc agatataatt ggaacagcac tattggactt cccgctataa aataatgtag
     1801 gtaaatcttg ttgtgacatc atgatcattc ctttcatttc attcgtttta actatcaatt
     1861 ttattttaaa caagataact atcaaataaa atgataacgg tttcatctat ctattttatc
     1921 ggtctagtgg ctgatttcaa gctagaaata ttgaatgaca atacaactct gttaaaatga
     1981 tggacgtaga caaatatgcg tattgacgct ttattttaaa aattaacatg cttataacat
     2041 gtttatagaa ggagattaac ctatgaacta tcaagttctt ttatattata aatatatgac
     2101 gattgatgac cctgaacagt ttgctcagga tcacttagcc ttttgtaaag cacaccattt
     2161 aaaaggtaga attcttgttt ctacagaagg tattaacggc acattatctg gtacaaaaga
     2221 agaaaccgaa caatatatgg cacatatgca tgccgatgaa cgattcaaag atatggtgtt
     2281 taaaattgat gaagctgaag gacatgcttt taagaaaatg catgtacgtc ctcgaaaaga
     2341 aatcgttgct ttagatttag aagatgacgt cgatccaaga cacacaactg gccaatattt
     2401 atcacctgta gaatttagaa aagctcttga agatgatgac acagtcatta ttgatgcacg
     2461 taatgattat gaatttgatt taggtcattt ccgaggtgca attcgtccaa atatcacacg
     2521 ttttagagat ttgcctgact ggattaaaga gaataaagcg ttatttgcag ataaaaaagt
     2581 ggttacgtac tgtactggtg gcattcgatg cgaaaaattt tctggatggc ttttaaaaga
     2641 aggtttcgaa gatgtagctc aacttcatgg cggtattgct acatatggta aagatcctga
     2701 aacaaaaggt gaatattggg acggtaaaat gtacgtattt gatgaccgta tcagtgttga
     2761 tatcaaccaa gttgaaaaaa caattattgg taaggattgg tttgatggca aaccatgtga
     2821 acgttatatt aattgcgcta acccagaatg taataaacaa atattagttt ctgaagaaaa
     2881 cgaaactaaa tatttaggtg catgctctta tgaatgtgct aaacatgagc gtaatcgtta
     2941 tgttcaagca aataatatta gtgataatga gtggcaacaa cgtttaacaa actttgatga
     3001 tttacatcaa catgcttagt tttaattaaa tacctttcaa aacacgcttt gaaaatccga
     3061 tttataaagg tttttcaagg ctgtttttta tgcgcataca taacctgata tgtttttaat
     3121 tatgcgcgtg ttatactaaa ttaaatttta atactgcggg gtgtcttaaa atgcacattt
     3181 tagtaacagg gtttgcgcct tttgacaatc aaaatatcaa tccctcatgg gaagctgtga
     3241 ctcaactaga agatattatt ggcacacata caatcgataa attaaaacta ccaacctctt
     3301 ttaagaaagt agataatatt ataaataaaa cgttggcatc taatcattat gatgttgtac
     3361 tagctatagg acaagctggt ggtagaaatg ccattacccc agaacgtgtc gccattaata
     3421 ttgatgatgc acgtattcca gataatgatg attttcaacc tattgatcaa gccattcact
     3481 tagacggtgc gccagcttat ttttcaaatt taccagttaa agcaatgact caaagtatta
     3541 ttaatcaagg acttcctgga gcactttcaa atagcgcagg tacatttgtt tgtaatcaca
     3601 cactttatca cttaggttat ttacaagata agcattaccc tcacctacga ttcggattta
     3661 ttcatgtgcc atacatacca gagcaggtca ttggtaaacc cgatacacca tctatgccat
     3721 tagaaaagat agttgcaggt ttgactgcag ccattgaagc tatttctaat gatgaggatt
     3781 tacacctagc cctgggcaca acggaatagt tataagtgca caaacaaaag caccctaatt
     3841 tgttagttta agtctaactt tagggtgctt ttggatgatt ggtatacagt tttactataa
     3901 tgatgtattt tagctaaacg acattgttta gtcttctttt tttaacttcc taaaatttac
     3961 aaatgtataa ataataaatg ctatatttag aatgatgagt atacttactg aaactaaatt
     4021 aaaagtgtct ggttctttac taaagatagc tgctatcctt gctatcaaac ttggcaatga
     4081 aattaccaac acgatgagcg cataaatttt ctgtttaatt aaaatacaag ttccaatgag
     4141 cgcaattaaa agtactaacc caacgatgaa actctgtttg tcacttaact caaagaaact
     4201 atagatagga tattttttaa taatcaagcc acctaaaatc atccataaaa atacgataat
     4261 tccataagtc acatttataa catacgttat tttttggtca ccaaatcgga ctaatgtatt
     4321 tcgtagaatc agcataccaa tgacaacacc taaaataacg atactagcta tataaagtaa
     4381 aaatgcaatt gtcacatcaa atgtacccaa atctaaaaac ctaggaatta taatgactgc
     4441 taaaataaaa gcgaagtaca aagtaatata tttatacaaa ccggtagtaa gacttatctc
     4501 aggtgataat tgatcagcca ttgacttaat cggtgtatta ataattgaac ttgtatcttc
     4561 gttatttttt tcagccatag ttaaatgatc ttcgagctct tccaataact cttctacttc
     4621 tgcttcagtc ttacctctaa ataacaattc aacacgtaat ttttctaaaa aatcttgaga
     4681 ttgtttactt aacatcgttt tcccctccaa acaagttaat catcccttta ttcaaaactt
     4741 gccatttcga tttaaatact tttagttcct ttaaacctga atcggtaatc gtatagtatt
     4801 tccgcctcgg gccgccatta ctagattttt ttattgtcgt atcaacgtat ccttttttgt
     4861 ttaaacgcat taaaactgga taaatactac cctcacttat ctctggaaac tcttgattct
     4921 taagtttcgt cataatttca tatccatacg tttcgccttg ggcaatgaga cctaatatcg
     4981 ccccatctaa gagacctttc ataatctgat ctgacactga cattttaatc acctactatc
     5041 ttacataata agatagtaca ttgagaactt ttcgtcaact atcttttatt gtaaggtagt
     5101 tgttgtacac attccttaaa tgactaacaa ctttgttaat agggtaatac ttacggaagt
     5161 atattttatt tatgggggag gaattaataa tgactacaaa aacagtattt gatgtcattg
     5221 atatggggtt aggatattta gtaaatgtgt atgatgcttg gaaagttgaa aaggtacttg
     5281 atgattatca taagcctttt tctaatacca ttcattggca atttggtcat gtattaacaa
     5341 tttttgaatc ggccttagct gttgctggta aagagaatat tgatttaaat atctatagac
     5401 ctttattcgg aaatggttcg tctccagatg aatggaagga tgaagtaccg agtattgaaa
     5461 ggattttaga aggtctccaa actttacctg aacgtgcacg aaatctaact gaagatgatt
     5521 tagcaattga attgaaacag ccaattgtcg gttgtaataa cttagaagag ttattagtat
     5581 taaatgccat tcacatccca cttcatgctg gtaaaattga agagatgtct cgtatattaa
     5641 aaaatttaaa ataaatatgt gcttattaac cgttaacaac acgttaacgg gttttttatt
     5701 tgtttaaaag gtcacttttt tgaatttaat aaacaccatc tataccagtt cttcaccgat
     5761 tctcgaaaaa taattatatt aatgatttcg ttaatttaat tttatattta attattactg
     5821 tacatctttt gtagttagct ttattcttaa attgaaatat gtataaaaca tagaaagaag
     5881 gacgttttaa catggaaaac acggttaaat atcgtaagtt tatactccct atcgttgtag
     5941 gtctccttat ttgggcactt acacctttta aaccggatgc tgtggatcca acagcatggt
     6001 atatgttcgc aatattcgtc gcgacaatca ttgcttgtat tacacaaccg atgccaattg
     6061 gggccgtctc tataattgga tttacaatca tggtactcgt tggcattgtt gacatgaaaa
     6121 cggctgtcgc tggttttggt aataatagca tttggttaat tgctatggca tttttcattt
     6181 cgagaggatt tgtgaaaaca ggtcttggta gacgtatcgc acttcatttc gtcaaattat
     6241 ttggtaaaaa aacattagga ttagcatatt ctatcgtcgg tgtagattta attctagcgc
     6301 ctgctacacc aagtaatacc gcgcgtgctg gtggaatcat gttcccaatt atcaaatcac
     6361 tttctgaatc atttggttcg aaaccgaaag acggatcagc acgcaaaatg ggtgcatttc
     6421 ttgttttcac agaattccaa ggtaatttaa ttactgcggc tatgttttta actgcaatgg
     6481 ccggtaaccc ccttgcacaa aatttagcat ctagcacatc taatgttcac attacatgga
     6541 tgaattggtt tctagctgct ttagttcctg gacttgtttc cttaattgtt gtacctttta
     6601 ttatttataa aatttatcca ccaactgtta aagaaacacc aaatgctaag agttgggctg
     6661 aaaatgaatt agcgactatg ggtaaaatcg ctttagctga aaaatttatg attggtattt
     6721 ttgtcgttgc gttaacacta tggattgtcg gaagtttcat tcatattgat gcaactttaa
     6781 cggcctttat tgcgctagca ttgttattat tgacaggcgt cttaacatgg caagacattt
     6841 taaacgaaac aggtgcttgg aacacattag tatggttctc agtattagtg ttaatggccg
     6901 accaattaaa caagcttgga tttattcctt ggttaagtaa atccattgct acaagtcttg
     6961 gtggcttaag ctggcctata gtcctggtca ttttaatatt gttctacttc tattcacatt
     7021 acttatttgc aagttctaca gcacatatca gtgcgatgta tgcagcatta ctaggcgttg
     7081 ccatcgcagc cggtgcacca ccattattca gtgcattaat gttaggtttc ttcggtaacc
     7141 tattagcttc aacaacacac tatagtagtg gtccagcgcc gattctattc tcttcaggtt
     7201 acgtgactca aaaacgttgg tggacaatga acttaatatt aggtttcgtc tactttatta
     7261 tctggattgg tttaggatca ctttggatga aagtaattgg tatattttaa aatatttaaa
     7321 ttagcgctcg aatctcattg atttgggcgc tttttaattt gtatttaaaa tcaacctttg
     7381 ctaaatcaag actccctttt taaaatacgt ttatccttta aatcattgcg tgcttcactg
     7441 aaaatttgta taaagattta agtcattacg taacatcaca taaaatacat ttctatacta
     7501 ttccgcttca ttgattaaca ttacgtatgc cctcataaat catcatacaa aaaacacctt
     7561 cgtttaaatt cattttaatt gcgaattcaa cgaaagtgcc ttatttcata tttaatgttt
     7621 caaatttata cgtctgtcac tgttactgca cacatacctc agtctcacct catcatgaca
     7681 caaaggatat tgaaaacacg tataattatc tttcctcgta ttttataggg tttttgcgac
     7741 cggatgtttc ttcaatttaa tgtattgaga aagactatat aacacaatac ctgtccaaat
     7801 aaatataaac gtaattaatt gatctatact aaaaggctct ttgaaaacaa atatgccgag
     7861 tacaaacatt attgttggtc caacgtattg aataaatcct attagcgaaa gtggaatacg
     7921 ttttgccccg gctgagaata ggattagtgg tattgccgta atagcaccag aaaataacaa
     7981 ccaaaatgat gacatgttca atccaaatga catctgatgt tgctgccata aataaataac
     8041 gtatattagt ccagcaggtg cggtaacaat acattcaatc gtaatactgc tgatggcatc
     8101 aatatgtact acttttttca ataatccgta tgtaccaaag gataacgcta atataataga
     8161 gacgattggg aattctccaa tcttgagcgt catatataat acaccgatga atgcgaataa
     8221 aatggctagc cattcaaatt tattgaatct ttcttttaaa aagataagtg cgagcaaaat
     8281 gctaacaagt ggatttatat aataacctaa acttgtttgt aggacgtgac cgttcgttac
     8341 agcccaaata aatgtacccc aatttaatgt aatgacatag cctgctacga caatcgctaa
     8401 tagctgaatg ggcttgccta acaattgatt catatctcgt tgaaatgcat tgcgttgttt
     8461 ttgtccaacc gcgagtatga aaatcatgaa tattgctgaa aatataatac gaaaggctaa
     8521 aatttcaaat gcgcctattg catcaacgaa ctgccaatat ataggtagta ttccccacag
     8581 aatgtatgca ctgagtgcta aaaatatgcc ttttttatac tctgaattca ccttcaaacc
     8641 tccttacttt cctaattttt aatttactgc atacgctcac ttggttatgc taatataacg
     8701 attttactaa taatatttcg ataaagatat cattttgttt atatttccca catttattca
     8761 ccaaccacta aacaatatta attttataaa taattctgta caaatcaggg tatattgcca
     8821 gaaagactac catacaacat aaaggatgga tacaaatgac tttacctaaa attggaaagc
     8881 ctgcaacacg cgcgctaaat tcacaaggta tatacacatt agaagcagta tcacaatata
     8941 cgaagtcatc tctaatggag atgcatggcg ttggtcctaa agctatatca atattggaac
     9001 aagctttatt tcagcaccaa ctacatttta aaacggaagt tcagtcatca ttacctttta
     9061 agttaacagg agatgtgtca tgcaatcatg cgcctaaacg tcaacaaatg atagatttta
     9121 tagttgttac agcagcatta gatattgaat tattacgttc actggttaca acagaattta
     9181 tttggtctgt ccctggccgc tttgacatat acggacctca aatattaatt caagaactat
     9241 ctaatcatta caatcaggtt gctagtctca atatccattc cagcatcaca catgggtgct
     9301 taggttccat gcatggtatt gaaatattaa aaactggtaa agagattcat tttgctcatt
     9361 tttttgaatt tgaaaatcat aaaaaggatg ccaagttaag caaagtgaca tcttacattg
     9421 ttgttggtta atttatcaaa ttatatatat cgcaaaaaat cttttgtggt tacatgtata
     9481 tttttatata acaaataatt tggcataaca tttttgttat atgaacactt attactagag
     9541 tgcccattga acatacagta tgattaaata tcatgttatg aaagaaggat atattttgac
     9601 tgtttttaaa aatgagcgct taagctggtt accatacata gctattgtca ttttgttaca
     9661 cgttattggg tttagttttt tatggattgc tggaaaagac catcatatct tatttggtat
     9721 ggggattctt gcatatacat taggtttgcg tcatgcattt gatgcagatc acattgctgc
     9781 aatagataat acggttcgca aattattaca gcaacgcaaa gatccatctg gtgtgggttt
     9841 ctatttttca attggacatt catctgtcgt atttttaatg gctgtttttt taggggtatc
     9901 tgtaaaatgg gctaaagatg aattaccgca tttccaagat attggtggaa cgattggtac
     9961 actagtttca ggtttctttt tagtgcttat cggtgtgttg aatctaatta ttttaatctc
    10021 tttaattaac ttgttcgcta aattacgtcg tgaacacatt gaagaagctg aagtcgatgc
    10081 attacttgaa tctagaggat tggtttctcg atttgtagga ccttatttca aattaatcac
    10141 gcgtagttgg cacgtattgc cacttggctt tttatttgga cttggttttg atacagctag
    10201 tgaaattgcg ttactcgctc tttcttcagg cgcatcacaa caagccattt catttatcgg
    10261 aatcttatct ttaccaattt tattcgcatc aggtatgagt ttattggata cattagatgg
    10321 tgttgtaatg aagtatgcct ataattgggc attcttcaat cctattcgca aaatctatta
    10381 caatataacg attactgcga tatcagtcat ggcagcatta gtgattggga tgattgaatt
    10441 gctacaaatt cttgctgata agttagattt acatggtgcg ttttgggcat tcattggttc
    10501 gatcgaattt gattatttag gctatatttt agttgcatta tttttaatta cttggcttat
    10561 ttcaagttta atttggaagt ttggtcgcat agaacacaaa tggtctagat aaggcaactt
    10621 gtcttgcaac tacctttgta ttgttgcatt tttagacacc ctgcacactt cttccattat
    10681 gctacgctct cagtagtcat ctttaaatcg tttcttttag agatgacttc attttgagag
    10741 cgtatttttc tatttaaaat gtatttatcc aaaattcaat gctttcgaat ttaaaatttc
    10801 agtatttctg tttaaaattt aattataatc tttaatacag gaggcgctat gcttatgaat
    10861 attgcgaagt tagagaatta tttacaaatt gattcatctc gttataatcg accgagtatt
    10921 gaagcattaa attattatgc aacacgtttt atgttaactg taccttttga aaatattgat
    10981 gttcaaaata gtaagccgat atctataaat atcgatgcac tttttaataa aattgttcat
    11041 gataaacgtg gtggtttttg ttatgaatta aatacatttt tcaaagccta tttacaacaa
    11101 aaaggattta atcctgaatt aatgtcagct actattcaca cacccggtgg cggtcgtagt
    11161 ctgaacggtt cacatgcttc acttgttgtt tctataaatg atgtattcta tgtaactgat
    11221 gttggctttg gagacttacc tctacatgcc attcctatta cttcatctga gcatacacaa
    11281 ccaattactg atatcagtgg tacattccgc gctattttta acaacgaaga caaggatatt
    11341 ttctatgttc aaaaattcga aaatgatcat tggcatacaa aatatgaagc tgaattcaaa
    11401 cctaaacaaa tagaagattt taatagcaac atcgaatata atcaaacgca tccagattca
    11461 atatttgtac agcatttatt aattacaatg ccacaatcat ttggccgtgc tacaatgtct
    11521 gaaaatcatt taactttaac aaggaatggt agctcagaaa aatttgatgt cactaaagat
    11581 aactataaac attttttaga aaaatatttt ggactaaatg taactattaa tcgtattgaa
    11641 aaacaataat agtaaaatat aaaaatatgg gcgtatctta cttattgtag gtagcgcccc
    11701 ttttttatgc ttcataaaca caacaaaaag gatatgacac aaacttcatg tcacaccctt
    11761 cttttgaacc gcttcatttt acttaagcaa gtgcatctac aacataaatg acacctacga
    11821 atacaaatgc aatagcaatc atcgtcgaaa taaagacgat acccattaat ggattaaata
    11881 ataaaatgac accgaaaaca atacctaata aattaaaaat cactgaaatt agttttaagc
    11941 taccactacc cgaaaacgta aataatccag agatagaact aaaaataaac caaaaagcaa
    12001 acatataaat aaagaatgct gaacttgcgc caacattaaa aataactaat agaccaaata
    12061 gaatatctac aatccccata aacagaatcc aattttggtt accacctact aaagcttttg
    12121 cttttctacg gtaaacgatt tgaatcacac cgttaattaa tacaaacagt ccaatcaacc
    12181 aggtaatagc ataaaaattt tcaattggaa atgtaaaaat aacgactgcc aacattaata
    12241 ataatacacc cattattaaa ctagaccatt taataccttg atttcctttt tccataacaa
    12301 tcaattcctt tcaaataaaa ccctctttta atttttacaa aaaatttatt atacaataat
    12361 taaattccct tttctcaatt tacacaaaca tcatcactta gaaagcacca ttgcttttgt
    12421 ctaatatgta cgatgaatct gtatcctcac tcaaaaatct actaagtagc gatagctttc
    12481 aactctgtaa ggttcattca ttgaattgta agtttagata aaggctacta ctccaaacat
    12541 tatttaaaat gaaattaaat acaaaaggag tgagactatg acgatattat cagtgcaaca
    12601 tgtttcaaaa acatacggta aaaagcacac atttcaagca cttaaagata ttaactttga
    12661 catacaaaaa ggcgaattcg ttgcgattat ggggccttct ggatcaggta agacaacctt
    12721 attaaatgta ctaagttcaa ttgaccaaat ttctagcggt agcgtgattg ctaacggaca
    12781 agagcttaat aaacttaatc aaaaagcact tgccaaattc cgcaaagaat cattaggttt
    12841 catcttccaa gattacagta ttctgccgac attaaccgtt aaagaaaaca ttatgttacc
    12901 tttatctgtt caaaaaatgt cgaaggcaac aatggaagaa aattataaag cgatcacgac
    12961 agcattaggt atttatgacc taggaaataa ataccctagc gaattatctg gtggtcaaca
    13021 acaaagaact gcagcagcga gagcatttgt tcacaaacca caaatcatat ttgcagatga
    13081 gccaacaggc gcactcgact cgaaaagtgc aaatgaccta ttacaacgtt tggaagaaat
    13141 gaataaatcg tttgatacaa ctattgtcat ggttacacat gatccggttg cagcaagttt
    13201 tgcagaacgt gtcatcatgc taaaagatgg ccaaattcat acacaacttt atcaggaagg
    13261 acgttctaaa caggcctttt atgaagacat tgtacatctt caatcagtat taggtggtgt
    13321 ctcaaatgac atttaaccat atcgttttca aaaacttacg acaaaactta aaacattatg
    13381 caatgtattt attttcatta ttttttagca tcgtcttata tttcagtttt acaaccttac
    13441 agtttactaa aggtgtaaat aatgacgact cgatggccat cattaagaaa ggtgctttag
    13501 tcggatcaat atttttattt atcattattg tcatcttttt aatgtatgcc aatcatttat
    13561 tcgtaaaacg ccgtacacgt gaatttgcgc tatttcagtt gattggttta acacgacaaa
    13621 acattttgaa aatgttagca cttgaacaaa tgatcgtatt tttaatcaca ggtgttgtcg
    13681 gtgtattatg tggcattgca ggtgcacaat tattgctttc catagtatct aaattgatgt
    13741 cgttatcgat taacttatcg atacatttcg aacctatggc ccttgtttta actattttca
    13801 tgctaattat tgcgtatgta ctgattttat ttaaaagtgc tttatttcta aaaagacgta
    13861 gtatcttatc aatgatgaaa gattcaatta aaactgatgc cacaactgct aaagtaacga
    13921 ctgcagaggt tatttcaggc gtattaggta ttgctatgat tgcactaggc tattatatgg
    13981 cgacagaaat gtttggtacg ttcaaagcac taactatggc tatgacatca ccgtttatca
    14041 ttttattttt aacggttgta ggcgcctatt tattcttcag aagttccgtg tcacttattt
    14101 ttaaaacatt gaaaaaatca aaaaatggac gcgtatctat taccgatgtt gtattcacat
    14161 cgtctattat gtacagaatg aagaaaaatg ccatgtcttt aactatcatt gcaattattt
    14221 ctgcagttac agtaactgta ttgtgttttg cggcattatc taaatcaaat acagatcaaa
    14281 cccttacatc tatggcacca aatgaattta acgtggtcgc tactcaagat gctaaacaat
    14341 ttgaaactaa actaagccaa caacaaatta cattttctaa aaatgcttac gaaactatta
    14401 ctgtagataa tgttaatgat caggttatta ctttggaaaa tggtagtgat tcaggtcgca
    14461 cgaattctat tttaagtgca aataacaagt taacaggcaa caatgccatc attacaaata
    14521 caaaatcgct gcctaacatt attaatatcc atttaaacaa agatttagta gtaaaaggta
    14581 ctaagaatga aactttccgt gttacacaag aagacaaagg taaggtttat cctttaaatc
    14641 taagcttcaa ctcacctgtc atcgaagtaa gtcctgaaaa gtatcagcag ttgaaaacac
    14701 aaaataacgt tcatactttt tatggatatg atattaaaca aacatcacaa aaggaaaaag
    14761 ctcaagcaat tgcaaaacag tttggagaca aagtcatcac gtatgatgaa atgaaaaaag
    14821 aggtcgatgc tactaacggt attctaatat ttgttacatc atttttagga ttagcatttt
    14881 tagtagcagc tggatgtatt atttatatta aacaaatgga tgaaactgag gatgaactaa
    14941 gtaacttcag aatattaaaa cgcataggct ttacgcatac agatatgctt aagggattat
    15001 tattaaaaat tactttcaac ttcggtttac cattattgat tgctatatta catgcagtat
    15061 tcgccgcaat agcatttatg aaattgatgg gtaatatttc cttcatgccg gttattgtag
    15121 taattgttgt atacaccctc atttacatta cttttgcttt aattgctttt gtacattcaa
    15181 acaaattgat taagaaaacc atttaacact tacgattaaa agatgcttca ttagatattt
    15241 tgattttaag caatgcagta taactattaa ttgatttaa
//
LOCUS       DAKTLH010000058        14970 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.058, whole genome shotgun sequence.
ACCESSION   DAKTLH010000058 DAKTLH010000000
VERSION     DAKTLH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14970
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.058"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(212..1330)
                     /gene="mnmA"
                     /locus_tag="O0394_001463"
     CDS             complement(212..1330)
                     /gene="mnmA"
                     /locus_tag="O0394_001463"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCY0499547.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            complement(1331..2473)
                     /locus_tag="O0394_001464"
     CDS             complement(1331..2473)
                     /locus_tag="O0394_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCY0499548.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMTIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            2785..3798
                     /locus_tag="O0394_001465"
     CDS             2785..3798
                     /locus_tag="O0394_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCY0499549.1"
                     /translation="MAIKYSALNLVPIREGEDEQTAINDMVNLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEK
                     NTL"
     gene            complement(4035..4181)
                     /locus_tag="O0394_001466"
     CDS             complement(4035..4181)
                     /locus_tag="O0394_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499550.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
     gene            complement(4221..4403)
                     /locus_tag="O0394_001467"
     CDS             complement(4221..4403)
                     /locus_tag="O0394_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCY0499551.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKVIGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            complement(4503..4925)
                     /locus_tag="O0394_001468"
     CDS             complement(4503..4925)
                     /locus_tag="O0394_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCY0499552.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            5010..6284
                     /locus_tag="O0394_001469"
     CDS             5010..6284
                     /locus_tag="O0394_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCY0499553.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDDENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            complement(6445..7218)
                     /locus_tag="O0394_001470"
     CDS             complement(6445..7218)
                     /locus_tag="O0394_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HCY0499554.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(7218..7346)
                     /locus_tag="O0394_001471"
     CDS             complement(7218..7346)
                     /locus_tag="O0394_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499555.1"
                     /translation="MGFIAFNKCFLVEFWENNQSDNAKYKILSYYVLMYKSVKEVK"
     gene            complement(7411..7601)
                     /gene="ssrS"
                     /locus_tag="O0394_001472"
     ncRNA           complement(7411..7601)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="O0394_001472"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(7679..9445)
                     /gene="aspS"
                     /locus_tag="O0394_001473"
     CDS             complement(7679..9445)
                     /gene="aspS"
                     /locus_tag="O0394_001473"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCY0499556.1"
                     /translation="MSKRTTYCGLVTEVFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            complement(9461..10723)
                     /locus_tag="O0394_001474"
     CDS             complement(9461..10723)
                     /locus_tag="O0394_001474"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCY0499557.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TASRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            complement(11184..12059)
                     /locus_tag="O0394_001475"
     CDS             complement(11184..12059)
                     /locus_tag="O0394_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCY0499558.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            complement(12056..12508)
                     /locus_tag="O0394_001476"
     CDS             complement(12056..12508)
                     /locus_tag="O0394_001476"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCY0499559.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ"
     gene            complement(12520..14709)
                     /locus_tag="O0394_001477"
     CDS             complement(12520..14709)
                     /locus_tag="O0394_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCY0499560.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
BASE COUNT         4609 a         2914 c         2120 g         5327 t
ORIGIN      
        1 taacaaaatt aaattcgtta tccccaactg gcattgcctg tagaatttct ttacgaaatt
       61 ctctatgttg tggtcccgcc aatataacat tgtagagcct aggacattgt gatgtcccag
      121 actctatcct catgaattat tctcatcaaa aactgtcttt cgtcattttc aacgttgaaa
      181 cttcaaataa gtaatttatt gttgccattg tttatacaac ataatttaat tgaccttcat
      241 ttttgaacac atcgtcaatt gttgcaccac caagacacac atcaccttga taaaaaacaa
      301 ctgcttgtcc aggtgtgatt gctcttactg gctcagcaaa agtaacacgt agtgcatggt
      361 cgttttcacg tttcacaaaa actttcgtat ctttttggcg atatctaaat ttagctgtac
      421 attcaaaacc ttgatctaag tcattatctt ctggatttac aaatgaatag tctgaagcaa
      481 ttaagtaatc actgtataat gcatcgtgat ggaatccttg ttctacatat aaaacattat
      541 cttttaggtt tttaccgaca acaaaccaag gatcgccatc tccacctata cctaatccat
      601 gtctttgtcc tattgtgtaa tacatcaaac cactatgttt acccattttc ttaccatcaa
      661 gtgttatcat atcacccggt tgtgcaggta aatattgtga taaaaatgtt ttaaagtttt
      721 tttcgccgat aaaacaaatg cctgtagaat cttttttctt agcagtaaca agtccttgtt
      781 cttcagcaat tcgacgcact tcactctttt cgatgtcgcc aattgggaac atcacttttg
      841 aaagttgttg ttgagataat tgattcaaga agtatgtttg atctttatta ttatctacac
      901 cacgtaacat ttcaacatga ccatcttcat gacgatgtat gcgtgcgtaa tgtcctgttg
      961 ctacataatc tgcacctaaa ttcatcgcat gatctaaaaa ggctttaaac ttaatttctt
     1021 tattacacat aacgtctgga tttggagtac gacctttttt gtattcatct aagaaatacg
     1081 taaagacttt atcccaatat tctttttcaa aattaacagc gtaatacgga atgccaattt
     1141 gattacacac ttcaataaca tcgttgtaat cttcagttgc agtacatacg ccattttcgt
     1201 cagtgtcatc ccagtttttc ataaatatgc caatgacatc ataaccttgt tcttttaaga
     1261 cgtgggctgt tacagaacta tctacaccgc ctgacatacc aacgacaaca cgtatatctt
     1321 tatttgacaa ttatgactcc tccttaaatt taaaatatat tttatgaatt tcagctacaa
     1381 ttgcattaat ttcattttca gtagtcaatt cgttaaaact aaatcgaatc gaatgatttg
     1441 atcgctcctc atcttcgaac attgcatcta aaacatgcga cggttgtgta gagcctgctg
     1501 tacatgcaga tccagacgac acatagattt gtgccatatc caacaatgtt aacatcgttt
     1561 caacttcaac aaacggaaaa tatagattta caatatggcc tgtagcatcc gtcattgaac
     1621 catttaattc aaatggaatc gctctttctt gtaatttaac taaaaattgt tcttttaaat
     1681 tcattaaatg aatattgtta tcgtctcgat tcttttctgc taattgtaat gctttagcca
     1741 tcccaacaat ttgcgcaaga ttttcagtgc ctgcacggcg tttcaattct tgttcaccgc
     1801 caagttgagg ataatctagt gtaacatggt ctttaactag taatgcaccg acaccttttg
     1861 gtccgccaaa cttatgagca gtaatactca ttgcgtcgat ctcaaattcg tcaaacttaa
     1921 catcaagatg tccaattgct tgaaccgcat caacatggaa atatgcattt gtctcagcaa
     1981 taatatcttg aatatcataa atttgttgca ctgtgccaac ttcattattt acaaacataa
     2041 tagatactaa aatcgtctta tctgtaattg tttcttcaag ttgatctaaa tcaatagcac
     2101 ctgtatcatc aacatctaga tatgttacat caaaaccttc tcgctctaat tgttcaaaaa
     2161 catgtaacac agaatgatgt tcaatcttcg atgtgataat gtgattaccc aattgttcat
     2221 ttgcttttac tatgccttta attgccgtat tattcgattc tgttgcgcca ctcgtaaata
     2281 taatttcatg tgtatctgca ccaagtaatt gtgcaatttg acgtcttgac tcatctaaat
     2341 atttacgcgc atctcttccc ttagcatgta ttgatgatgg attaccataa tgcgaattgt
     2401 aaatcgtcat catcgcatct actacttcag gttttactgg tgtggtcgca gcataatctg
     2461 cataaatttc catgtttgga cactcctcac aattttatca atgttccaat aatagcacct
     2521 tacatactat ttttctactt ttctgtttaa ctttatttat aatgttttta attatatttt
     2581 accattttct acacatgctt ttcgataggc ttttttaagt ttatcgcttt attcttgtct
     2641 tttttataaa ttttagtatt tgcagatatt tttttatttg taaaatgtaa cgtactatta
     2701 ttttggttat gagcaattta atatttatct ggttattcga ttggtatact tcttatatca
     2761 taaaaaagga aggacgatat aaaaatggcg attaaatatt cagcattaaa ccttgtccct
     2821 attcgagaag gtgaagatga acaaacagca attaatgata tggttaatct cgcacaacat
     2881 ttagacgaat tatcatatga aagatattgg attgctgaac accataacgc tcccaaccta
     2941 gtaagttcag caactgcttt attaattcaa catacgttag aacatacgaa acacatacgt
     3001 gtaggttctg gaggcatcat gttacctaat catgctccat taatcgttgc ggaacaattt
     3061 ggcacgatgg caacattatt tccaaatcgt gtcgatttag gattaggacg tgcacctgga
     3121 acagatatga tgaccgcaag tgcattaaga cgagatcaac atgatggtgt ttataaattt
     3181 ccagaagagg tttcattatt acaacaatat ttcggccctg ctcaccaaca agcatatgtt
     3241 cgtgcttatc cagcagtagg taaaaatgtg cctttataca ttcttggttc ttcaacagat
     3301 tctgcacatt tagctgctcg caaagggctt ccatatgtgt tcgctggaca ttttgcacct
     3361 caacaaatga aagaagctat cgaaatttac aaaacgttat ttgaaccttc tgatgtatta
     3421 gacgaacctt atgttattgt atgtttaaat acaatcgttg ctgaaaatga tgacgaagca
     3481 caatatttag cttcatctat ggcacaagta atggttagta tcactcgtgg cagaatgcag
     3541 cccgttcaac cgccaacaca tgaactacaa aatatattaa cgccgagaga atacgcgatg
     3601 gctatggaaa gacagaaaat atcattaata ggttcagaaa atactgttca acaaaaaatt
     3661 caagatttta tggaaactta tggtgaagtc aacgaaatta tggcaataag ttatatttat
     3721 gataaagata tgcaattaga ctcttatcgt cggttcaaga atgttataaa tcagataaat
     3781 gaaaaaaaca ctttataatg tgataaataa actaagtgaa agtatgtatc cataatatta
     3841 ataaaaatat acagtaacag cattttgaat gaaagatgtc tttattgttc aatcatttat
     3901 tttagtaatg attcaaattc acttaaaatt ctaatgcaaa tatgaaagcg ccccttcaac
     3961 tttacactgt gtaagtgttt atttgatggg gcgctttcaa aatattgaaa agcatatcca
     4021 aaatttaaag aaatttattt ctctttatct tcattttctt ttttctcttc gttattcgat
     4081 cctgtatatt catttatctt atcttttaca tttttaactt gttcattatc gctattttta
     4141 aatttttcta ctgcgtcttt agctttatcc ataaaactca tattaatcgc tcctcttata
     4201 tttgattagt ttaattgaac ttatttttta agtttatcaa ttgcatcagt tattttgttt
     4261 ttagcatttt caacaacttc ttttgcttta ccaatcactt tatcttgctg accttctttt
     4321 tctaattctt tgttatcagt aacgttacct actgtttctt taacatttcc tttaaattga
     4381 tcgaacttac tttcgtctgc catagtgaaa cctccttgga tgtatatatt tatataccac
     4441 taaggaggtt cgctaaacat tataatatga agtttttatg ttatagtata gtatttatac
     4501 gattaaatat aaaacatgta tccgtctaaa tcttcacttg tatctacata ttccgctaaa
     4561 tatttcaatg ttgtattatc taaaacatct ctcactgcat ctctcatgcg aatccatagt
     4621 tgtttttgcg caggtggttc tgattcaata ctttcaacaa atgtaattgg accttctaac
     4681 agtcttataa tatcccctgc tgagatttct tccgctggca ctcttaattg gtatccacct
     4741 ttagcaccgc gtacacttcg aattaacccc gcatttctta aaggacctac aagctgttct
     4801 aaatataaat cactcaaatt attttcttca gcaattgact ttaatgatat acatccttgc
     4861 ccctcttttt tagcaagaga aatcatcaat gtaagtccat atctcccttt agtagaaatt
     4921 ttcattgtat aacctcactt aattcgaata ttgatattcc cattttagca ttttttgagt
     4981 taagatagta taagaaaggt gtgacaaatg tgagtacaga accattagca tcgagaatgc
     5041 gcccaaaaaa tatagatgaa atcatttccc aacaacattt agttggacca agaggcatta
     5101 tcagaagaat ggttgataca aaaaaattaa cttcaatgat tttttatggt ccacctggta
     5161 taggcaaaac aagtattgcc aaagcaattt cgggcagtac gcaatataaa ttcagacaat
     5221 tgaatgctgt aactaacact aaaaaagata tgcaacttgt tgttgaagaa gctaaaatgt
     5281 ctggtcaagt tatcttgtta ttagatgaaa tacatcgact agataaagct aaacaagact
     5341 ttttattacc tcatttagaa aatggcaaaa tcgtcttgat cggtgctaca acttcaaatc
     5401 cttatcatgc tatcaatcca gcgattcgtt caagagcgca aattttcgag ttatatcctt
     5461 taaatgacga agatgtgcgc caagcgttaa ctcgtgcaat agaagatgat gagaatggtt
     5521 tgaaaacata tcaacccaaa attgatgaag atgccatgac ctacttttct acacaaagcc
     5581 aaggtgatgt tcgtagtgcg ttaaatgcat tggaattagc tgtattaagc gcagataatg
     5641 acaaagacgg ttatcgacat gttacattgc aagatgctaa agactgttta caaaaaggtg
     5701 catttgtaag tgacaaggat ggtgacatgc attacgatgt tatgagcgct ttccaaaaat
     5761 ctatccgtgg tagcgacgtc aatgccgctt tacattattt agcacgatta attgaagctg
     5821 gagatttacc tacaatagtt cgacgattac ttgtaattag ctatgaggat ataggcttag
     5881 cctcacctaa tgctggtcag agaacacttg ctgctattga atcagcagaa cgtctaggtt
     5941 taccagaagc tagaattcca ctaagccaag cagtaatcga actatgctta tcacctaagt
     6001 caaattcagc aatgagtgcc attgatagtg cattgtccga tattagaaac ggtcatgtgg
     6061 gccaaattcc aaaccattta aaagatggac attatcaagg tgctaaagat ctaggccgat
     6121 ctattggtta caaatatcca caccaatatg ttaatggcta tgtttcacag caatatttac
     6181 ctgataaact aaaaaacaaa atttattacg aaccaaaaac gacatctaaa agtgaacaac
     6241 aactcaaaga aatatataac aacttactta aacaaaggcc gtaaaaataa gattaaatcg
     6301 attacctttg atattttagc tataatcaaa tcaaaactga ctagtacacg aggcatattc
     6361 tactatgctt gcgaactagt cagttttagt tttatggtgt ggttacactt gccttacgtt
     6421 gaaaattatc cctttcaaaa tgaattattg ccctttgtct ttaatgcgac gaactggaat
     6481 atcttttaat acgtcattca ccacataact tgcacaaatt aatccaacaa cacttggcac
     6541 aaaggcattt gaagaaggtg gcatttgtcc ttttcgattg atagcatttt tatctccaac
     6601 tatatctttt acatcttctc ttatgacaat tggactttca tctgaaaata caactggaat
     6661 ccctttacga attcctagtt ttttcaattt ttgacgaata attttggcca ttggatcggt
     6721 atgtgtttta gagatatctg aaattgtaaa acgtgttgga tctgttttat ttgcagcacc
     6781 catactggaa atcactttaa tccctcggtc aagacactct ttcattaagt gtactttgta
     6841 cattattgta tcacttgcat ctacaaaata atctatatcg tagttatcga aaatttcttc
     6901 atatgtctct tctgtataaa acatatgtaa gggcgtgact ttacaatctg gattaattaa
     6961 tttaatacgt tcttccatca aagaaacttt actttgtcct accgttgtag ttaaagcgtg
     7021 taattgtctg tttacatttg taatatcaac atcatcttta tctattaata taatatgacc
     7081 aatattcgtt cttgctaatg cttcagcagc aaatgaacca acacctccaa cgccaagtat
     7141 gacaacagtt tgttgcttca ataaatctaa accttgttgt ccaatcgcta gttcatttct
     7201 tgaaaattga tgtttcatta ttttacctct ttcactgatt tatacataag tacatagtaa
     7261 cttaaaattt tatatttagc attatcactt tgattatttt cccaaaattc aacgaggaaa
     7321 catttattaa acgctataaa acccaactaa ttctttatta aaaacttaaa gaaacgcata
     7381 aaaatacgca agacaaagtc ttgcgtatcg atagagtccg tattgccgta gttataatag
     7441 cttgatcatt cggcctgtta tatacaggtg ggtgccctgt ttcttgtttt gtacgtcctt
     7501 catataaggc gtgtacgctg caagaaaacc cattgggctc ccttgatcaa agagtgttag
     7561 gcccaaatta aaaagcaaac ttacgaacaa ctcagatgac tatcttatga tgttatatta
     7621 ccacataatt aaaattaatg aaattataac aaaccaaagt ttattgattt tttaaaattt
     7681 agtgacgaat tcgcaaagaa agttcttcta attgtttatc agaaacttca ccaggcgcat
     7741 tcgttaataa acatgtagca gatgctgttt tagggaatgc gattgtatct ctcaagtttg
     7801 ttctattagt caataacatg actaatcggt ctaatcctaa tgcaataccg ccatgtggtg
     7861 gtgcaccata tttaaatgca tctagtaaga agccgaactg ttcttgtgct tgttctttag
     7921 taaatccaag aacttcgaac attttttctt gtaactcacc atcatgaatt ctgattgaac
     7981 cgccacctaa ttcataacca tttaatacta tgtcataagc atttgcctca gcttcttctg
     8041 gcgcagtgcc aagcttagca atatcagctt cttttggaga tgtaaatgga tgatgtgctg
     8101 caacgtaacg tttcgcatct tcatcatatt ctaataatgg ccaatctgtc acccataaga
     8161 agtttaattt tgtttcatcg attaaaccta attctttagc taatttgaca cgtaatgcac
     8221 ctaaactttg tgcaacgaca tttggtttgt ctgcaacaaa cattactaag tcaccagctt
     8281 cagcaccagt taatgtaagt aatgtttcaa cattttctgt ttcaaagaaa cgtccaattg
     8341 gacctgtcaa accatcttcc acaactttaa cccacgctaa tcctttagca ccatagatgt
     8401 ttacaaattc tgttaaagca tccatatctt tacgagtata ttgttcagct gcacctttag
     8461 cgacaattgc tttaatttca ccatcatttt caacagtatc tttaaatact ttaaagtcca
     8521 tatcacgtcc taattgagaa acgtcaatta attccatttc aaaacgtgta tctggtttat
     8581 cagaaccata gcgacgcatc gcttctttat atgtcatgcg tgggaaagcg ccattaattt
     8641 caacgccttt aacttcttta acaacttttt taagcatttc ttcacccatt tgcatcacat
     8701 cttcttggtc tacaaaactc atttcaatat cgacttgtgt aaattcaggt tgacgatctg
     8761 cacgtaaatc ttcgtcacgg aagcatttta cgatttggta gtatttgtca aatccactaa
     8821 tcatcaataa ttgcttaaat aattgtggtg attgtggtaa tgcataaaat tcaccatcat
     8881 gaacacgaga tggtactaaa tagtcacgtg caccctcagg tgttgacttc gttagtactg
     8941 gtgtttcgat gtcaaagaac ccttcatcat ccaaatattg acgaatagaa cgtgtaattt
     9001 gatgtctcat tttaaatgtt tgcgctaact cttgacgacg taaatctaaa taacggtatt
     9061 ttaatcgaat attttcatca acgttaacat tttcttcatt tatagaaaat ggtggtgtct
     9121 cagatttatt aatcacttta atatttgtaa cttgtacttc aacttggcca gttttaattt
     9181 taggattaac tgtttcaggg tcacgcttcg taactgtacc ttgaacttct acaacatatt
     9241 cagaacgtac tgtttcagca attttcaatg cctcttctga aaatgcagga ttaaacacga
     9301 cttgtacaat tccttctcta tctcttaaat caacgaaaat caatccacca aggtcacgac
     9361 gattgttaac ccatcctttt aatgtaattt cttgtcctaa aaatacctca gtaactaatc
     9421 cacaataagt tgttctctta ctcatatttt aacgccctct ctacttctta aaatattcga
     9481 ctaatgcgtc taattcaatt gtttcagatt caccagttgt catattttta acatcgattt
     9541 tattattttc taattcttga tcaccaataa cgattgtaaa cttggcacct aaacggtctg
     9601 cttgtttcat ttgtccttta attttacgct gtaaatagtc tttatctgct ttaataccat
     9661 tatgtctcaa atgatttaat agcttcacag catatcgatc tgcttgatca cccattgtaa
     9721 caatgaataa atctaagttt tcttcaatat ctaattcgat accttcttct tcaagtgcaa
     9781 gcaataatcg ttctatactt agcgcaaaac caatacctgt ttcacttgga ccatctagca
     9841 attctaataa accattataa cggccaccac cacaaagcgt tgtaatggca ccatcatagt
     9901 tagggttatc catcattaat tcaaatgctg tatgtgtata ataatccaat ccacgaacta
     9961 agttaggatc ttcaatatat ggaataccta aatcatctaa ataagctttt acttgttcat
    10021 aatatgcctt agattcctca tttaagaaat cagtgattct agatgcagtc ttaatcgctt
    10081 ctttatcacg gtctacttta caatccaaaa ttcgcatcgg atttgtatgc aaacgtgatt
    10141 gacaatctga acaaaattca tgaattactg gttcaaagtg tttcactaac gcttcgttat
    10201 attcttttcg agacgccata tcccctacac tattaataac aagctttaaa tgttttaatc
    10261 caaatgattg ataaatatgc ataaccatag ctaatacttc tgcatctacg ctaggatttt
    10321 cagcaccaat agcttctaca ccaaattgat taaattgacg atagcgtcct ttttgcttac
    10381 gttcatatct aaacatcggt ccattgtaat aaagtttaat tggttggttt ggattacctt
    10441 gcattttatg ttcaatatat gaacgcacaa ctgcagctgt tccctcaggt cttaatgtaa
    10501 tacttctatc gcctttatct ttaaatgtat acatttcttt ttgtacgaca tcggttgaat
    10561 caccaacacc tcttgcaaaa agatctgtac tttcaaaaat tggtgttctt atttctttat
    10621 aattataaaa tgtcattaat tcatctaatt gattttcaat gtaacgccat ttctttgaat
    10681 cttcaggtaa aatatcctgc gtccctctag gtattttaat cattttgaca tctccctcgg
    10741 caaactattt tatttaatta cctttttaaa actaaaacta tatacgacca cttaactttg
    10801 cacgataaat cataatctta aaagcaaaag aaaaagccct tgcacagcct gaaactgcac
    10861 aagggacgaa tatccgtgtt gccaccctca ttggtttaaa aaacatgcct aattgtaatc
    10921 atcattactt tttataagcg tgcatttaat aaacccactc aaaacgttta acgtacgttt
    10981 aacggcttta cttttcataa agcacctctt cttgtgttca atgtatataa actactcagc
    11041 ataatctttc agtctatgga ttatattcct acacaatctt agttagattg ctacctattt
    11101 catttaccat ttcaagaata ctaacggttt tagttataaa attcaatatc aatgataact
    11161 actttcacat atttttgcaa gccctacgca gaaaaataaa ttttaaggcc atcaacaatt
    11221 gcttgttcta aaatttgtct atgtaattga tctttaatca tcgtttcatc agttgggtta
    11281 ctaatataac ctaattctaa taaaacagca ggaacttttg tttgtcttaa cacttgataa
    11341 ttttcttgtc ttgaaccgcg attagaaagt agacctttct tctgaatcgt agcgtctaac
    11401 gtatctgcta aagctctttg attatcatga taccaataaa ctgtcattcc atttgcatta
    11461 gatgattcta acgcatcatt atgtatactc aaataggcat cgcctttgat atcacgattt
    11521 tctagtgaaa catatgtatc gtctgttctt gtcatcttaa cagttgcgcc ttctttttct
    11581 aaagtacgct gcaattcttt tgctgttttc aacgtataat ctttttctaa acttttatat
    11641 ttagtattgc ttgaagcacc ctggtcacta cctccatgac caggatcaag cactattgtt
    11701 ttaccttgca aaggattttt ctccttcgta ttatccgcga caatatctaa atttgtgtgc
    11761 catccagcta tccaaccttt ttcattactg gatgtatctt caacttcaat ccatttacct
    11821 actttaccaa tctttttaaa atggtcacct ttttcaactt tatatatgac tggatacgca
    11881 gcgtttggac ctgtacgtaa ttcagcattt tcagttatcg tgatgttccc actatcttca
    11941 ctattgctat tcagcaataa aaataaaaag atgataaata agacaaaggc aatcactact
    12001 attagagtac gtttattttt aagacccttt ttagataacc atgcctctat ttttttcatt
    12061 gaattttgcc gtcctgactt tcataaataa tagtgactgg accatcatta tttatgctaa
    12121 cattcatgtg tgttccaaat tcacctgttt tcacagtaag accatacgct cgtagcgcat
    12181 cattaaaata ctcataaatt tttaccgctt gatcaggatt tttagaattt gagaaacctg
    12241 gacggttacc tttttttaca tctgcataga gagtaaattg tgaaactgat agtatttcac
    12301 cattcatttg ttggatatta aagtttaatt tattattgtc atcttcaaat aatcttgcat
    12361 tagcaatttt ctttgcaatt acatctgcat cttgctctgt agagttctga ccgataccga
    12421 ctaataaaca atatcctttt ttgatttgat tatttaatgt atcattcgtc accgatgctt
    12481 ctttaactct ttgtacaact actttcatat tttgcacctc tagttccaaa ctcttgttac
    12541 tgtataaaca tcaccaagtt gtttgatctt ttctaccaca cgataaacat cattcacgtt
    12601 tttcaccatg acactaatat ttattattgc atttttatca atatctgaac gtcctgaaac
    12661 tttaattaaa ttgccggctg tcgagctaac agcttgtagt acttcattca acaagccatt
    12721 tcggtcatac gcagttacct ctaaatcaac ctgatatttt tgagttgcgt cttttgattt
    12781 tacccattca acattaatta gtcgttcagt ttcgttctta atatttgggc aatcagtgcg
    12841 atgtacttta ataccgtgac ctttggtgat ataacctaca atatcatcac ctggtatagg
    12901 attacaacat tttgacaact tgataagtac attttctaaa ccttctacat agacaccact
    12961 atcagtaatg atgttgtctt taataggcaa tgatttcgta acttcttgtg cttcatttaa
    13021 agcacgttgt ttatctaaaa tacgttgtct ttcagttaat ttattaacaa tctgtaagga
    13081 tgtcacgccg ccaaatccta cagctgcgaa taaatcatct tcatttgcaa agttatattt
    13141 ttcattaaca acctgaatat ttttctctgt caaaatatct tcgactctaa atccttgctc
    13201 ttttatttca gcttcaacca tcattcggcc tttttcaata ttagatgaac gatcttgttt
    13261 tttgaagaaa cttttaattt tacctttggc actagacgat ttaacaattt tcaaccaatc
    13321 acgacttggt ccatatgaat gtttactagt acgtatttca acaatatcgc ctgtttgtaa
    13381 aatatagtca attggtacaa ttttgccatt caccttggca ccaatcatct tattacctac
    13441 ttcactgtga atcgcataag caaaatcaat cggcacagca ccatatggca actcaataac
    13501 atcactcgct ggggtaaatg cgtatacttt gtcactctgt aagtcatatt ttaaggtttc
    13561 cataaattct tgagcgtcag acgatgtatg atccgcttca gctaattctt ttaaccaatt
    13621 taacttattt tgataagttt gatctttttc acttactttt ttaccttctt tgtaagccca
    13681 gtgtgctgca acaccatgct cagcaatttc gtgcatatca aacgttcgta tttggatttc
    13741 gagcgggtct ccatttgggc ctactactgt agtatgcaat gactgataca aattttgttt
    13801 aggcattgca atataatctt taaaacgtcc tggcatcggt ttccataacg tatgcaccaa
    13861 cccaagtatc gcataacaat cattaataga attgacaata acacgtatcg ccaacaaatc
    13921 aaaaatttga tcaaattgtt ttttctgctt catcattttc cgataaatac tgtaaatatg
    13981 tttaggtcta ccatttatat cgccttcgat attcattcgg tccatttcag tacgtattct
    14041 atcaatagcc gtttcgatat acgcttcacg ttcactacgt ttcttcttca ttaaattgac
    14101 tattctaaaa tattgcacat tatcaatata acgaagagcc gtatcttcta gttcccattt
    14161 gattgtatta ataccaagac gatgtgctaa tggtgcataa atttctaatg tttctcgaga
    14221 aattctaatt tgtttttcgc gcggcatggc tttcaaggta cgcatattat gtaatctgtc
    14281 tgctaatttc accaaaatta cgcgtacatc tttggcaatc gcaataaata acttgcgatg
    14341 attttcagct tgttgttctt cttttgagcg gtattttact tttttaagct tcgtcacacc
    14401 atcaacaatt cgagcaactt cttcattgaa catttctttt acatcttcaa atgtatacgg
    14461 tgtatcttca attacatcat gcaaaaaacc tgcgacaatc gtcggtccgt ctaatcgcat
    14521 ttctgttaaa atacctgcaa cttgtatagg atgcataatg tatggtaatc cgttttttcg
    14581 gaactgacct ttatgtgctt cataagcaat atgatagctt tttaaaacat actcatattc
    14641 atctgctgac aaatatgatt ttgctttgtg aagaacttcg tctgcactat atggatattc
    14701 gttgttcatt atatgataca ccccattcat atttattact tcgccttaaa caatgattta
    14761 ggtactcttg ttgaatagta tttgtcccac accaatcata cgtccgtcga cgataaatat
    14821 ttatcctgtc gtcattaatc gtaatattaa ttttacttga gcgagtttaa tttgtatact
    14881 attcctactt ttaaaacttt tacaaaaatt cgacctaaat ctactgtttc attttttaaa
    14941 tattagttct atgatactac aatttatgaa
//
LOCUS       DAKTLH010000059        14646 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.059, whole genome shotgun sequence.
ACCESSION   DAKTLH010000059 DAKTLH010000000
VERSION     DAKTLH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14646
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.059"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..69)
                     /locus_tag="O0394_001478"
     CDS             complement(<1..69)
                     /locus_tag="O0394_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCY0499561.1"
                     /translation="MESTLELTKIKEVLQKNLKILII"
     gene            complement(414..3023)
                     /gene="adhE"
                     /locus_tag="O0394_001479"
                     /gene_synonym="adhC"
     CDS             complement(414..3023)
                     /gene="adhE"
                     /locus_tag="O0394_001479"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCY0499562.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQKTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
     gene            complement(3473..3957)
                     /gene="tnpA"
                     /locus_tag="O0394_001480"
                     /pseudo
     CDS             complement(3473..3957)
                     /gene="tnpA"
                     /locus_tag="O0394_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500412.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(4261..4515)
                     /locus_tag="O0394_001481"
     CDS             complement(4261..4515)
                     /locus_tag="O0394_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499563.1"
                     /translation="MLIKIRGGNMKRDFFEKWSFILNLILFCAFLFLVVLMFYKDVDL
                     IYIFCTLLISLLPLLNIIKRIGWEINQSSMIYEVILAVID"
     gene            complement(4671..5579)
                     /locus_tag="O0394_001482"
                     /pseudo
     CDS             complement(4671..5579)
                     /locus_tag="O0394_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            complement(5804..6016)
                     /locus_tag="O0394_001483"
                     /pseudo
     CDS             complement(5804..6016)
                     /locus_tag="O0394_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc peptidase"
     gene            complement(6091..7626)
                     /locus_tag="O0394_001484"
     CDS             complement(6091..7626)
                     /locus_tag="O0394_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCY0499564.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMVFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELL
                     PVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVKNDEVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            complement(7677..9221)
                     /locus_tag="O0394_001485"
     CDS             complement(7677..9221)
                     /locus_tag="O0394_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0499565.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVKYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDNDGKSL
                     KASSLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQNEKVTFDIPQNVSVNEPFEMTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNVTIKVNAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            9450..10406
                     /locus_tag="O0394_001486"
     CDS             9450..10406
                     /locus_tag="O0394_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0499566.1"
                     /translation="MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            10620..11393
                     /gene="phnC"
                     /locus_tag="O0394_001487"
     CDS             10620..11393
                     /gene="phnC"
                     /locus_tag="O0394_001487"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCY0499567.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            11395..12195
                     /gene="phnE"
                     /locus_tag="O0394_001488"
     CDS             11395..12195
                     /gene="phnE"
                     /locus_tag="O0394_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCY0499568.1"
                     /translation="MPLEIPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            12192..13007
                     /gene="phnE"
                     /locus_tag="O0394_001489"
     CDS             12192..13007
                     /gene="phnE"
                     /locus_tag="O0394_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCY0499569.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(13138..14316)
                     /gene="deoB"
                     /locus_tag="O0394_001490"
     CDS             complement(13138..14316)
                     /gene="deoB"
                     /locus_tag="O0394_001490"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCY0499570.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
     gene            complement(14344..>14646)
                     /gene="deoC"
                     /locus_tag="O0394_001491"
     CDS             complement(14344..>14646)
                     /gene="deoC"
                     /locus_tag="O0394_001491"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCY0499571.1"
                     /translation="VKVIIETVLLDHDEIVKASELTKTAGADFVKTSTGFAGGGATAE
                     DVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADSDY"
BASE COUNT         4611 a         2706 c         2224 g         5105 t
ORIGIN      
        1 aataattaaa atcttcaagt ttttttgtaa tacttcttta atttttgtta attctaatgt
       61 actttccatt atttacctcc cttaaaaatt ttcattaaaa ttggtattat atatatagta
      121 tttacatatt acatatcgtt tgaacaatta attactttta aaccgcatta ttttcgcctt
      181 tttatttata tgaaattaaa aataattata ctctaaagta gtaatagttt gtgaaatgag
      241 tatcaatctt aatattttct ttatttaaaa aaatgattgt ctagtttgta tctctctgaa
      301 gatttggcaa taaataaaag ccgataaccg tataatgatt atcgacttaa agtttatgtg
      361 gcatttttta cttttgtaat ttcaggtgag ttagatgatt attatcagat agattattgc
      421 ttataatcat atgatgtttg aatgatatct ttgatttcac tgattagtgc ttctttagga
      481 ttagcagttg tacattgatc ttcaaatgcg agctctgcca ttctatcaat tgactcattt
      541 aattcttctt cagacacacc ttgtgatttc aaattcattt caattccgac tgattgacct
      601 aattcgtaga cagctttagc taatgattct acgagtgctt ctgtcgtatt accttttaat
      661 cctaagaatt tggcaatatc tgcataatct gtatctgctc tgaagaactc atatttaggg
      721 aataatgcat gtttttgcgg gtctttggca ttataacgga taatatgcgg tagtaatatc
      781 gcattcgctc taccatgcgg aataccatat tcgccaccaa ttttatgcgc aattgagtgt
      841 gcaatgccta agaatgcatt tgcaaatgcc ataccagcca aagttgatgc gttatgcatt
      901 ttctctcttg aaactttatc acccttttca acagatgatt ttaaatattc gaacgtcaat
      961 ttaatcgctt gtagactcaa acctcttgtg tagtctgaag ccattacaga tacatatgat
     1021 tccattgcat gcgttagtac atccattcct gtatctgctg taacgctttt tggcacactc
     1081 atcacaaatt gagggtcaat aattgcaacg tcaggtgtta aagcaaaatc agccaacgga
     1141 tattttacat ttgtttcact atctgtgata actgcaaatg gtgttacttc tgaacctgta
     1201 cctgatgtcg tagggataca aatgaacgtc gcattttcag gcatgcctat tttataagta
     1261 cgtttaccga tgtctaggaa cttttgttta gcaccgaaga atgatgtctc agggtgttca
     1321 aagaacatcc acattgcttt tgcagcatcc atcgctgaac caccaccaag tgcaatgatt
     1381 gtatccggtt ggaaatcaac catcatttcc agacctttat atactgtatt agttgatggg
     1441 ttcggttcga cttcgctaaa tattttaatt tgaggctgtt ccgttttttg tcttaataca
     1501 ttctcaactg tttttgtata accgaattct accataccag ggtcacagac aatcatcact
     1561 ttttcaatct tgtccattgt tgttagactc atgattgcat tttcttcaaa ataaatttga
     1621 gcaggcacct tgaaaatttg agtattatta cgtcgtttag caatcgtttt aatgtttaat
     1681 aaatctgtcg cactaacatt atgtgaaatt gagtttctac cgtaggaacc acaacctaat
     1741 gttaaagacg gaatcaattc gttatacata tcaccaatac ctccaaccgc tgatggtgta
     1801 tttacaagta cacgacaagc tttcattctt agtccaaaat ctttttgtaa tgtttcatct
     1861 tctgtatgga taacggctgt gtgtcctaat ccaccaaaat gtagtgtgtc ttcacaaatt
     1921 tgaaatgctt gttttgtaga ttgggctttt actaaggcta atactggaga taatttttca
     1981 cgagataacg gatagtctga acctacaccg ctaatttcgg ctatgataag ttttgtattt
     2041 tcggggacag gtatacctgc taattcagct atttcaactg cagatttacc gacaatatca
     2101 ggcttaatac ctgttttttg ttcattcata attgcatttt ctaagcgttg taattcatct
     2161 tttttaacaa agtatgcttg atgtgcttta aattcattag taacatcttt ataaatttct
     2221 ttatcaatga ctacaacttg ttcagaagca caaatcatac cattatcaaa tgtttttgaa
     2281 ccaatgatat catttactgc acgtttaatg tgtgctgttt tttcaatgta agacggcacg
     2341 ttacctggtc ccacacctaa tgccggtttg ccagttgaat atgcagactt aaccatgccc
     2401 gaaccacctg ttgctagaac taatgcaata cctttgtgat tcattaattg ttttgttgct
     2461 tcgatagaag gcacttcaat ccactgaata atatctttag gtgcacctgc cttcattgcc
     2521 gcttctaata caacttctgc tgcacgcttc gacgattctt gtgcacttgg atggaatgca
     2581 aaaatgattg gatttcctgt cttaattgca atcatcgctt taaaaatagt tgtcgacgta
     2641 ggatttgttg ttggcgtaac accacaaata acaccaattg gttccgctac atacgttaat
     2701 cctttttctt tatcttcacc aataatccct actgtcttat tgtcttttat tgaattccat
     2761 atatattcag aagcgtataa atttttaatc gctttatctt cgtatatacc tcttccagtt
     2821 tcttcatgtg ctaattttgc tagcaccata tgttgatcaa cagctgctaa gctcatttga
     2881 tgaacaatat gatcaatttc ttcttgtgac tttttagata atgcttctaa tgcttttttc
     2941 cctttgtcag ctagagcatc aatcataatt gccacttctt gttctttcga tccacgattt
     3001 tctttttcag gtatagttaa catatacaac cactccttta tactttgtga attatttcac
     3061 aaacattata gtacatgtct ctcaggatat aaagaaaatt ctatacaaaa aagtttaatt
     3121 tcgaatatta tttgaacaaa tatcaaattt taaaataaat gttttcatga aatcattgtt
     3181 atttcggtgt ttttagaatg attttataat cataattttt tcaatgacat aatttattca
     3241 taattatata tttaattctg aatattcttt aatagcctaa aaagcttgtt atatcgcaca
     3301 aaacacaaca ttaaaaggtc cttgtcgata cctatcaaca gatgttacaa ataaaaacca
     3361 cccgtgtgaa cgggtggttt gttctgcggc tataagcctt ccttactggc cagccctaaa
     3421 agggcactga caagtcagcc aactgcacta ctattccagc aatcctaaag gtttactctt
     3481 ttttctttct ttttttattt ttctctccag tgaaaggatc taaatattct tccattgaaa
     3541 tttcatctgc aacgatatcc tcttgtaatt gattacgaat ataattttca atcacttttt
     3601 tatttctacc tactgtatcc acataaaatc ctttacacca aaactttcta tttccatatc
     3661 tatactttaa gttagcatgt ctatcaaata tcattaaatt acttttcctt ttaaatagcc
     3721 aacaaatgat gataccccaa gtttgggtgg tatactaact aacatatgga tatgatcttt
     3781 acatacctct gcttcaatta tctctacacc ttttctttca tataattgac gtaatataat
     3841 ccctatatct ttttttattt ttccatatat cgcttgtctt ctgtatttag gtgcaaagac
     3901 aatatgttac ttacaattcc atttcgtatg tgctaaactg tttgtgtcag atgacattaa
     3961 atagcatctc ctcgtgttga ttattttggt tggctgacca atatttactc taacatgtag
     4021 agatgcattt ttttgacaat ggtagaacct tttctgggga gtgggacaga aatgatattt
     4081 tcgcaaaatt tatttcgccg tcccacccca acttgcattg tctgtagaaa ttgggaatcc
     4141 aatttctctt tgttggggcc ccgccccaac tcgcattgcc tgtagaattt cttttcgaaa
     4201 ttctctgtgt tggggcccct gactagaatt gaaaaaagct tattacaagc gcattttcgt
     4261 tcagtcaatt actgccaata taacttcgta gatcatagaa gattgattta tttcccagcc
     4321 tattcttttt ataatattta gcaaaggtaa taatgatatt aataatgtac aaaaaatata
     4381 aatcaaatcg acatccttat aaaacatcag aaccactaaa aacaaaaaag cacaaaataa
     4441 aattaaattt aaaataaacg accacttttc aaaaaaatct cttttcatat ttccacccct
     4501 aattttaata agcattattt tatattctct tttaagttta ttattcaaaa ggaaaacaga
     4561 aatatctttc aatattatta taaacatttc aactactttt aaaaaccaac aaaaaaatac
     4621 ttattttaag tagatgagca taagtgaaca tagttcttta gttataataa ttaattcaac
     4681 caaaagtcga tttgtttttg caattggttt tcatttcctc ttaaagatat tttcattaaa
     4741 tctgtcaaat caatagacgc tatatttttc aacttatctc tatatttatt tttagtacgt
     4801 ctttctaaat ttccccattc ctcttcttcg tgagttaata aatgaagcat tgctcgttct
     4861 tgtatatttt caatcatttt taaattcggt tttaaaatat gcaaatcatc aaaacaatct
     4921 ttccaacaat caaccatatc tcgttttaat tcaatttcca cacgccatag aaatgttgaa
     4981 tcaatttcaa catctgcatt atctttacgt tcttgttttt attataaatc cgaataaacc
     5041 tatcactatt acgcacacca aaatattttg tttctggttt tacattacgt ccataaaata
     5101 tagttttctt taccgactta tctgacaatg cataatagtc atttaaatca aattcaaaat
     5161 caaaagccaa atctaatctc gtaaaactaa catcgtccaa ataactgatg atattttgtt
     5221 ttaaccaaag cacttcatca tgcgaaagct tattaggatt aaattcaacg cgcatactac
     5281 gtctattcca aagagttgct tttattttgt catattcaat ataaactttt tctttaagag
     5341 ctttagcttt aaagtttgtt tgtaaaatat cccaaagccg aatttcagga ttagtactca
     5401 taaaatgtga aagtctctct gcgttagaca tgctaagatt cccaacaatc gttatagcgt
     5461 caaaagacaa ttttggaata gctagtgaca tcctatgtcg atttaaccgg ctattaccgg
     5521 atattagagt atccagtttt acaaatggat gaaacgaaat tcaaaacact aaaaaatatg
     5581 ttccactaac agcaaaaaaa taccattatg ttcctactaa aaaactaaaa atactggaga
     5641 acaaatgtca ggatataact taggatacta tgtaataaaa atttacaata aaaaaacagg
     5701 aaaacaaatt tcaagtaaaa gaatacccat acaaagagga taaaataaaa aacctcgaac
     5761 tgaaatgatg atcttttcag ctcgaggttt aaatattggt gccttattta tatagattcg
     5821 ttatattata ttctctattt tcattaacat aatccttaaa gagttttaaa ttaatacctg
     5881 ctagatgatt caaaaatgtt tcatcaactt ttaaataatt caataatttt tgtggtgtca
     5941 gtaaatttct atcaaaatac aactttaata aactattcat tttgacagga cgtgacattt
     6001 caatcacgtc gtctaaagat aatactttct cgctttaaac aaaaacaaaa acttacccga
     6061 ttaaaatcaa gtaagtttta catatatccg tcacttttca actttaaaat caacaacttg
     6121 cgggatgcgc atcaaattat tatttgataa aaaatcaata agtaattgtg cgccttcaac
     6181 ttgaatatct tttacaactg gcgcgtcgat atacatatca tactgaccac cgcctactgc
     6241 acgataatta tttacacaaa ttgtatatgt ctgctttaaa tcaactgcgt gaccttgaat
     6301 catcatattg ctcacacgtt gtccctttgg tcttccaaca tgaatggtat aacttacgcc
     6361 accatatata tcataattaa agtgttgtgg tttgggttca aggaagtctg cgctcacact
     6421 aacttcatca tttttcacgt caaaatattc tgctgatcgt tcaatggctt ctttaagttt
     6481 ggcaccactt acagctaaaa ctttaaatgt atttggaaat gggtaattgt taataacatc
     6541 tcgcatcgtc acgacttgct tgaaaccact agcagaatca aacaaagctg tacaggcaac
     6601 atctgcgtca cttttttcta ataaagcgta attcataaaa tttgtaaaag gatgcggtgc
     6661 cacacgtgcc tcaaatgcat gattaatcgt catatcatat ggcaatgtag taatttcgta
     6721 atctaaccag tcctctaact gctttcgtaa atgttggtca tcttcatcaa tagtaaatgt
     6781 ggaatcatct ataacaggaa gtaattcaca tgattcaacg gatagatttt catattcatc
     6841 agtactcaag actactctgc ctacagttgt acctctcgta ccaggttgaa tcacagccgt
     6901 ttgcttaaac ctttcagcaa tttgtcgatg ttggtgaccc gtaataaaga tatctatatc
     6961 tttagaaaac gcttctaaca tggcatatcc ttcattttca cccgttaata cttcggtcgg
     7021 cgtaccactt tctaaatcct tttcaaatcc accatggtaa caaaccacaa tgatatctgc
     7081 atgtcgcttc atttcaggta agtattgttg aagtatttca aaagcactat gaaacgtaag
     7141 tgactgaata tgctctggtt gttcccaatg gggaataaat tgtgtcgtta aacctatcac
     7201 accaacagtt tgatctccaa cctgaaaata cttcacaccg ttatcagtca atgtactatc
     7261 attttcatat atattagcgc acaaaactgg ataattgagt ctgcgtaaag tgtcttttaa
     7321 gtatggtaat ccataattaa attcatgatt accaagcgta ccaaagtcga ataccattcg
     7381 attataaaaa tcaactaaag gctggctact gccgctatgc gcgattaagt aattacaaaa
     7441 tggtgaccct tgcaaaaaat caccattatc tattttaaaa ctttggtcat actgccttct
     7501 gtcttgttct ataacatgat tcgctagtaa caatcccata ggttgatatt gatttctact
     7561 cgtaaaatct gttgggaaaa tataaccatg tacgtcactc acgacataaa atgctatgtt
     7621 tgacatcctc actcactcct tcaatcacaa acatctttct tatttctatt atatatttat
     7681 ttgaagtctg ttgtaatcaa ggttttgtca ccgagtttta aacgaatctt tgaaccttcc
     7741 atactttcaa gtactttagc attgacctta attgtgacat ttccgttttc atctgcttta
     7801 actgttggca aagtactgta acctggtggg ttataatcgt tatctttact tgaaaattgt
     7861 ccgatttgac gtccgccttc tttgtaaata ccaactctaa gattttcaag tgtttgattt
     7921 gcttcaaatc cttttaaatg tattgtcatt tcaaatggct catttacaga aacattttgc
     7981 gggatatcaa atgttacttt ttcgttctga tttggtggtg tatgatcatc tggtgtgttt
     8041 ggctgaggat ctgcgccttt ttcgctgcca taactacctg ctttaaatgt tgttggatca
     8101 taccatttat aaccactcgg cggttgtgac catggctctt tttcaggctc agttgaacgc
     8161 tctggtcgtt caaaatcaag caacttagtc tttgtatcta atgttaggct actcgcctta
     8221 agtgatttcc catcattatc tttagacatc caagccgtta tattatttaa tagcttaccg
     8281 ttgtcttgtt ctttaaaacc atcatatgtt ttcttctttt ctccattatc ttctcttaca
     8341 tatttgggcg aactatcttc cacaagtgat gaatcaccga taaatgctgc tttacctttt
     8401 ccaactttag aaattgctac ataggggcct tctgctttac cgcccccatt ataaatacct
     8461 tgatctacag catgtgacca tttacttttc gctggcaatt gttctggtgt atacacaata
     8521 ccttttgctt tctctggatt agtaattgct aatgtcgatc cggcatgcat agagacagat
     8581 ttcacacctt cagtaatacc gaaactttct tttgaagaaa caatattgct cgtatttaaa
     8641 tcacctagtg cattatatcg aaaacgtacg ccaaagtttg tagataacca atctgaactt
     8701 ttcacacctt gcattgcagt agaacttttt tcttctgcat tcataccttt cgacatatct
     8761 tcatatgctc cacgtcgata accattcatt gcctccgatg aatcaatacg atttaaattt
     8821 cggtcagcat tgtaatgatc tgaaataaag acaacattgc caccttgttt cacatattta
     8881 acaattgctg cctgttctga ttctttgaaa ggaatgttag cctcaggaat tacaaatatt
     8941 ttggaacttt tcaaacttgc ttctgttatg ttcgaatgac catcaatagc tttaacgtca
     9001 taaccttgtt tttgtattga atccgcataa tctgaaaatg caccatcact aacccaatct
     9061 gcagcaccag ctgtttgacc atgagaacga tcgaataata ccgttcgctg ttgctttgta
     9121 ggttgcgatt catgcgttat agctaaagat tgcggtaaag cacttaatga taccgttgca
     9181 acaattgcag agacagttaa tgacttatat atttttttca ttttgtgagg ctccttttaa
     9241 aataaatttg ttcttgaatt ataggataaa aattcgttgc atatgagcaa tttaacgaaa
     9301 aatttacaaa atcttatcaa actcttaaag aaagttatta aaattcattt ttataaaata
     9361 ctttttaaca tttaaatgtg gtacgctata agtgtaattt cattgcatac atattacacg
     9421 attaagaatg tgaaggggac agttatcaaa tgaaaaattt taagtgttta tttgtattaa
     9481 tgttagcagt cattgttttt gcagcagcat gtggaaactc aagttcttta gataatcaaa
     9541 agaacgctag taatgattcg gattctaaat caggaggata caaacctaaa gaattaaccg
     9601 ttcaatttgt accttcgcaa aatgctggaa cattagaagc taaagcaaaa ccattagaaa
     9661 aattactatc taaagaatta gggattccag ttaaagtgtc tgtatcaact aactacaata
     9721 caattgttga agctatgaag tctaaaaaag ttgatgttgg tttcttacca ccaacggcat
     9781 acacattagc acatgatcaa aaagcagctg atttattatt acaagcacaa cgtttcggtg
     9841 taaaagaaga tggttcagca agtaaagaac ttgtagatag ttataaatca gaaattcttg
     9901 ttaaaaaaga ctcaaaaatt aaaagcttga aagatttaaa aggtaagaaa attgccttac
     9961 aagatgtaac atcaactgct ggatatacat tcccacttgc gatgttaaaa aacgaagcag
    10021 gtattaatgc aactaaagat atgaaaattg tgaatgttaa aggtcatgac caagcagtta
    10081 tctcattatt aaatggagat gtagatgctg cggctgtatt taacgatgca cgtaatactg
    10141 tgaaaaaaga ccaaccaaat gtatttaaag acacacgaat tttaaaatta acacaagcta
    10201 ttccgaatga cacaatttct gtaagaccag atatggataa agattttcaa gaaaaattga
    10261 aaaaagcttt tatagacatt gctaaatcaa aagaaggtca caaaattatt agcgaagttt
    10321 attcacatga aggatacaca gaaacgaaag attcaaattt cgacattgta agagagtacg
    10381 aaaaattagt taaagatatg aaataatcat tatttaacaa atgaatcatt agcgaatttg
    10441 gtattaaaag ctttcgttca atagatatat tctagattaa tattgaaaag ctaggcgcta
    10501 aactgaaaca gatatagaaa ggtgtcgctg tacatttgaa accatttgta cacagaaacc
    10561 caatgtctat gatatttcag tttaccttgg cttttcttta ttaaagaaag gtgtcaaaca
    10621 tgagtcaaat cgaatttaaa aacgtcagta aagtctatcc taacggtcat gtaggcttga
    10681 aaaatattaa cttaaatatt gaaaaaggtg aatttgcagt tattgtcgga ctatctggtg
    10741 ctgggaaatc cacgttatta agatctgtaa atcgtttgca tgatatcacg tcaggtgaaa
    10801 ttttcatcca aggtaaatca atcactaaag cccatggtaa agcattatta gaaatgcgcc
    10861 gaaatatagg tatgattttc caacatttta atttagttaa acggtcaagt gtattacgaa
    10921 atgtactaag tggacgtgta ggttatcacc ctacttggaa aatggtatta ggtttattcc
    10981 caaaagaaga caaaattaag gcaatggatg cactagaacg cgtcaatatc ttagataaat
    11041 ataatcaacg ctctgatgaa ttatcaggtg gccaacaaca acgtatatct attgcacgtg
    11101 cgctatgcca agaatctgaa attattcttg cagatgaacc agttgcttca ttagacccat
    11161 taactacgaa acaggttatg gatgatttaa gaaaaatcaa ccaagaatta ggcatcacaa
    11221 ttttaattaa tttacatttt gttgacttgg caaaagaata tggcacacgc atcattggtt
    11281 tacgtgatgg tgaagttgtc tatgatggtc ctgcatctga agcaacagat gacgtattta
    11341 gtgaaatata tggacgtaca attaaagaag atgaaaagct aggagtgaac taacatgcct
    11401 ttagaaatac ctacaaagta tgactccctt ttaaagaaaa aggtttcttt aaaaacgagt
    11461 tttaccttca tgttaatcat tgtgcttatc atttggagct tcatttatac gggatttaat
    11521 tttggagatt taatgatagg aatacctcaa ataggtgatc tattcaaaca aatgattcca
    11581 cctgatttcg agtatttaca acaaattaca acgccaatgt tagataccat tcgaatggct
    11641 atcgtaagta cagtattagg tagcatcgtt tcaataccaa ttgcgttatt atgtgctagc
    11701 aatatcgttc atcaaaagtg gatttcaata ccctcgcgct ttattttaaa tatagttcgt
    11761 actattccag atttgttatt agcagcaatc tttgtggctg tatttggaat cggtcaaatt
    11821 ccagggatat tagcactgtt tattttaact atctgtatta ttggaaaatt attatatgaa
    11881 tcattggaaa cgatagatcc aggtccaatg gaagcaatga cggctgttgg cgctaataaa
    11941 ataaaatgga ttgttttcgg tgttgtacca caagccatat cgtcatttat gtcatacgta
    12001 ttatatgcat ttgaagtaaa tatacgtgct tcagctgtgc ttggattagt cggcgctggc
    12061 ggtattggat tgttttatga tcaaacactt ggtttatttc aatatccaaa aacagcaacg
    12121 attattttat ttactttagt tatcgtcgtc gtcattgatt acatcagtac gaaagtgagg
    12181 gcacatctcg catgacacag gaaatagcaa aatataatgt tcacacaaaa gcacacaaac
    12241 gaaaattgat taaaagatgg cttattgcaa ttgtcgtctt agctattatc atctgggcat
    12301 ttgcaggtgt accaagttta gaacttaaaa gtaaatcatt agaaatctta aaatccatat
    12361 tcagcggatt attccatcct gatatcagct atatctatat accagatggc gaagacttat
    12421 tacgtggttt acttgaaacc tttgcgatag ccgttgtagg tactttcatc gccgcaatta
    12481 tctgtattcc attagcattt ctaggtgcaa ataatatggt aaagctacgc ccagtttcag
    12541 gtgttagcaa atttatttta agtgttatac gtgtcttccc agaaattgta atggcactta
    12601 tatttatcaa agctgttggc ccaggttcat tttcaggtgt attagcttta ggtatccatt
    12661 ccgtaggtat gcttgggaaa cttttagctg aagatattga aggtctagat ttcagtgctg
    12721 tagaatcatt aaaggccagt ggtgcgaata agattaaaac actcgtattt gcagtcatac
    12781 cacaaattat gcctgccttt ctatcactca tactttatcg ctttgaacta aacttacgtt
    12841 cagcttctat actggggcta attggggctg gtggtatcgg gacaccactc atatttgcca
    12901 ttcaaacacg ttcttgggac cgtgtaggta ttatattaat cggtttagta ctaatggtcg
    12961 caattgtcga tttaatttcc ggttcaatcc gaaaacgtat tgtttaacat taaatcagga
    13021 tactcctaaa taagaagtcc taccgtctta cgtttctcta ttataataaa aacagcagtg
    13081 aagaaaacta ttgttatagt taacttcact gctgttttta taatatctaa atttattcta
    13141 tttcaattcc tttaaataac ttttaccgaa ctctggtaat gttacgttga aattatctgc
    13201 tatagttgca ccgatagaac tgaatgtagt atcactttct agtgcatgac cacctttaaa
    13261 tttcggactg tacataatta ctgggatata ttctctcgta tggtccgtac ctggcgctgt
    13321 cgggtcatta ccatggtctg ctgtaataat tactaaatcg tcttctttta agttgctaaa
    13381 cagttctggc aagcgatcat cgaaatcttt aattgcttgt gcataacctg gtttatcacg
    13441 acgatgaccg tataatgcat caaagtctac taagtttaag aagctaatac ctgtgaaatc
    13501 tttcttaaca attttcatca attgatccat accgtccatg ttactcttcg tacgaaccgc
    13561 ttctgttaca ccttcaccat cataaatgtc attaatttta ccgatggcaa taacatcata
    13621 accaccgtct ttcaaatgat ctaagacagt tttaccaaaa ggttttaacg catagtcatg
    13681 tcgattagat gtacgtgtaa agtttcctgg ttcaccaaca tatggacgtg cgataatacg
    13741 accaattaaa tatttagggt cttttgtcaa ctcacgaacc ttttcacaaa tatcatataa
    13801 ctcttctaat gggataatgt cttcatgtgc agcaatttgc aatactgggt ctgcacttgt
    13861 ataaacaatt aagtcaccag ttttcatttg gtgctcgccc cactcatcga taatttgcgt
    13921 acccgatgcc ggtttgttag caacaacttt acgacctgtc atttcttcaa tttgttgaat
    13981 taactcttca gggaatccat tagggtatac tttaaaaggt tgcataatat ttaatcccat
    14041 aatttcccag tgaccagtca ttgtatcttt accaactgaa gcttcactca atttagtata
    14101 gtatgcttct ggttgttcaa ctgcatttac tactggtaat ttatcgatgt tccctagacc
    14161 taacttttca aggtttggta aagtttgatc gaaaccttct aaggtatgtc ttaaagtatg
    14221 tgaaccttca tctttaaaat cagctgcgtc tggcgcttca ccaataccta ctgaatccat
    14281 tacgattaaa tgtacacgat taaatggtct tgtcatagct atcactccca aaatttatat
    14341 atattagtaa tctgaatctg cttctaaacc ttgcataatt tgaacacctg cgctcgcacc
    14401 aatacgtgtc gcacctgctt caaccatttt attgaaatct tctaaattac gtacgccacc
    14461 tgatgctttt acttctacat cagcacctac tgtatctttc attaatttaa cgtcttctgc
    14521 agtcgcaccg ccacctgcaa aacctgttga agttttaacg aagtccgcac cagccgtttt
    14581 tgttaattca ctcgctttta caatttcgtc atggtccaac aataccgtct caataatcac
    14641 ttttac
//
LOCUS       DAKTLH010000060        14382 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.060, whole genome shotgun sequence.
ACCESSION   DAKTLH010000060 DAKTLH010000000
VERSION     DAKTLH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14382
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.060"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            78..1118
                     /locus_tag="O0394_001492"
     CDS             78..1118
                     /locus_tag="O0394_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCY0499572.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKENQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
     gene            1132..2199
                     /locus_tag="O0394_001493"
     CDS             1132..2199
                     /locus_tag="O0394_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HCY0499573.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPSEQIISRLKAANVKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVITKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELIHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            2266..2364
                     /locus_tag="O0394_001494"
     CDS             2266..2364
                     /locus_tag="O0394_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499574.1"
                     /translation="MRFYFTNLIKRKHQAEDRRKILIKQKHQTKVR"
     gene            2412..3260
                     /locus_tag="O0394_001495"
     CDS             2412..3260
                     /locus_tag="O0394_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCY0499575.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            3273..4100
                     /locus_tag="O0394_001496"
     CDS             3273..4100
                     /locus_tag="O0394_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCY0499576.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            4127..5446
                     /locus_tag="O0394_001497"
     CDS             4127..5446
                     /locus_tag="O0394_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCY0499577.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALNWIVTDPLESIKEEIEL
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLFGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            5530..6447
                     /gene="lipA"
                     /locus_tag="O0394_001498"
     CDS             5530..6447
                     /gene="lipA"
                     /locus_tag="O0394_001498"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCY0499578.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            6577..6960
                     /locus_tag="O0394_001499"
     CDS             6577..6960
                     /locus_tag="O0394_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCY0499579.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(7039..7305)
                     /locus_tag="O0394_001500"
     CDS             complement(7039..7305)
                     /locus_tag="O0394_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HCY0499580.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            7403..7837
                     /locus_tag="O0394_001501"
     CDS             7403..7837
                     /locus_tag="O0394_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HCY0499581.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVGIRKQFTYDYTALD
                     IKIIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            7837..8616
                     /locus_tag="O0394_001502"
     CDS             7837..8616
                     /locus_tag="O0394_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCY0499582.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            8649..9608
                     /locus_tag="O0394_001503"
     CDS             8649..9608
                     /locus_tag="O0394_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HCY0499583.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
     gene            9862..9972
                     /locus_tag="O0394_001504"
     CDS             9862..9972
                     /locus_tag="O0394_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499584.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            10020..10172
                     /locus_tag="O0394_001505"
     CDS             10020..10172
                     /locus_tag="O0394_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCY0499585.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            10188..11645
                     /gene="dltA"
                     /locus_tag="O0394_001506"
     CDS             10188..11645
                     /gene="dltA"
                     /locus_tag="O0394_001506"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCY0499586.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVNR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            11642..12856
                     /gene="dltB"
                     /locus_tag="O0394_001507"
     CDS             11642..12856
                     /gene="dltB"
                     /locus_tag="O0394_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HCY0499587.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            12874..13110
                     /gene="dltC"
                     /locus_tag="O0394_001508"
     CDS             12874..13110
                     /gene="dltC"
                     /locus_tag="O0394_001508"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HCY0499588.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            13107..14282
                     /gene="dltD"
                     /locus_tag="O0394_001509"
     CDS             13107..14282
                     /gene="dltD"
                     /locus_tag="O0394_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCY0499589.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKEYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
BASE COUNT         5169 a         2067 c         2653 g         4493 t
ORIGIN      
        1 ctggtttatt aaacgagaac gtcgggaatt aagtaactac aataaaaata agatatgaca
       61 ataaggagac tacacgcgtg atcattgcca taattatatt gatatttatt tcgtttttct
      121 tttcaggaag cgagacggca ttaacggctg ccaataaaac aaaatttaaa actgaagctg
      181 acaaaggtga taaaaaagca aaaggcattg taaagttact tgaaaaacca agtgagttta
      241 ttacaacgat tctaattggg aataatgtcg cgaatatttt attaccaaca cttgttacaa
      301 ttatggcttt acgttggggg attagcgttg gtattgcatc agctgtttta acagttgtta
      361 tcattttgat ctccgaagtg attcccaagt ctgtcgctgc aacatttcca gataaaataa
      421 caaggcttgt atatccaatt attaatattt gtgtcattgt gttccgtcct atcacattac
      481 ttttaaataa gttgacggac agtattaatc gaagtttatc taagggccaa cctcaagaac
      541 atcaattttc aaaagaagaa tttaaaacaa tgttagcaat tgctggacat gaaggtgctt
      601 taaatgaaat tgagacgagt aggttggaag gtgtcattaa ttttgaaaat ttaaaagtaa
      661 aagatgtaga tacaacacct agaattaatg tgacggcatt tgcttcaaat gcgacatacg
      721 aagaagttta tgaaacggtt atgaataagc catacactag atatccagtg tacgagggag
      781 atattgataa cattattggg gtgtttcatt ctaaatatct gttggcttgg agtaataaaa
      841 aagaaaatca aattacaaac tattcagcta agccattatt tgtgaatgaa cacaataaag
      901 ctgaatgggt attacgtaag atgactattt ctagaaaaca tttagcaatt gtgttggacg
      961 aatttggtgg tactgaagcg atagtgtcac atgaagactt aattgaagaa ttattaggta
     1021 tggaaattga agatgagatg gataaaaagg aaaaagaaaa actttctcaa cagcaaattc
     1081 aatttcaaca acggaaaaat cgcaacgtat ctatataagg agcgaacagc tatgtggaat
     1141 aagaatcgac ttactcaaat gttaagtatt gaatatccaa ttatacaagc aggtatggca
     1201 ggaagtacga caccgaaatt agttgcatca gtaagtaaca gtggtgggtt aggcacaata
     1261 ggcgcaggtt actttaatac gcagcaattg gaagatgaaa tagattatgt acgccaatta
     1321 acgtcaaatt cttttggcgt aaatgtcttt gtaccaagtc aacaatcata taccagtagt
     1381 caaattgaaa atatgaatgc atggttaaaa ccttatcgac gcgcattaca tttagaagag
     1441 ccggttgtaa aaattaccga agaacaacaa tttaagtgtc atattgatac gataattaaa
     1501 aagcaagtgc ctgtatgttg ttttactttt ggaattccaa gcgaacagat tataagcagg
     1561 ttgaaagcag cgaatgtcaa acttataggt acagcaacaa gtgttgatga agctattgcg
     1621 aatgaaaaag cgggtatgga tgctatcgtt gctcaaggta gtgaagcagg tggacatcgt
     1681 ggttcatttt taaaacctaa aaatcaatta cctatggttg gaacaatatc tttagtgcca
     1741 caaattgtag atgtcgtttc aattccggtc attgccgctg gtggaattat ggatggtaga
     1801 ggagttttgg caagtattgt cttaggtgca gaaggggtac aaatgggcac cgcattttta
     1861 acatcacaag acagtaatgc atcagaacta ctgcgagatg caattataaa tagtaaagaa
     1921 acagatacag tcattacaaa agcgtttagt ggaaagcttg cacgcggtat caacaatagg
     1981 tttatcgaag aaatgtccca atacgaaggc gatatcccag attatccaat acaaaatgag
     2041 ctaacaagta gcataagaaa agccgcagca aacatcggcg acaaagagtt aatacatatg
     2101 tggagtggac aaagcccgcg actagcaaca acgcatcccg ccaacaccat catgtccaat
     2161 ataatcaatc aaattaatca aatcatgcaa tataaataat cgaccgcaat ccacaaaagc
     2221 acaagcaccc ccaaacatta ttttagtgct tgccattttt gtggattgcg tttctatttt
     2281 acaaatttaa tcaaacgaaa acatcaagct gaagatcgcc gaaagatttt aatcaagcaa
     2341 aaacatcaaa ctaaagttcg ctgaaatgat tatgataaaa gttatatggt atgatgacat
     2401 tggtgatata tatgataaac atcggattaa caggttgggg tgatcactat tcattatatg
     2461 aagatttaga acgccaaacc gataaactta aaacatatgc tggacatttt ccggttgtcg
     2521 aattagatgc gacatactat gcgatacaac cggaaagaaa tatattgaaa tggataaaag
     2581 aaacgcctga tacatttgaa tttgtggtca aaattcatca agcactcaca ttgcatgcag
     2641 actacaaaac atttgcagat acaaggcaag aactatttga tcaatttaag aatatgttag
     2701 agcccttaca tacacagaaa aaattagcaa tggtattggt tcaatttccg ccatggtttg
     2761 actgcaatgc acaaaatatc aaatatattt tgtatgtaag acagcaatta caagcatttc
     2821 caatgtgtgt agaatttagg catcaatcat ggtttagtga tgcatttaaa gaacaaacat
     2881 tggcattttt aacagaacat caaatcattc atgcagtagt tgatgaacca caagtgaaag
     2941 atggcagtgt acctttagtc aatcgaatca caaatgaaat tgcgtttgta cgttatcatg
     3001 gacgtaatca ttacggttgg actaagaaag atatgtcaga tcaagaatgg cgcgatgtac
     3061 gctatttata tgattataat gagcaagaat taatagactt ggcacaaaag gcacaaatat
     3121 tagcacaaaa agctaagaaa gtttacgtca tatttaacaa taattctggt ggtcatgcag
     3181 caaataatgc caaaacatat cagcgattat tgaatataga atatgaaggg ttagcaccac
     3241 aacaattaaa attattttaa gaggcgacga ctatgttatt aacaattaca ttattagttt
     3301 taatcggagg tttgtcagcg attatagggt ctatcgtagg cattggaggc ggtattatta
     3361 tcgttccaac aatggtttac ctcggtgttg aacatggatt actacataat attacaacac
     3421 aagtagcgat agggacgtct tcagtcattc taattgtgac aggactttct tcatcacttg
     3481 gatatttaaa aacaaaacaa gttgatatta aaaatggttc catcttttta tttggactat
     3541 taccaggttc attgcttggg tccttcatta gtagatattt aacatttgag tcatttaatt
     3601 tatattttgg tatcttttta attttcgtag ccattttatt aatggtaaga aataagatta
     3661 aaccgtttaa aattttcgat aaacccaagt atgaaaagac ttatgtagac gctaaaggta
     3721 aaacatatca ttatagtgtt ccaccattgt ttgcttttat tacaacgttt ttaattggta
     3781 tattgacagg tttatttggt attggaggtg gcgcactaat gacgccacta atgcttattg
     3841 tatttagatt tccacctcat gtagctgttg gaacaagtat gatgatgatt ttcttttcaa
     3901 gtgtcatgag ttctataggg cacattgctc aaggtcacgt agcttggggt tatgcaatca
     3961 ttttaattat ttctagttat tttggtgcga aaatcggtgt caaagtgaat caatcaatta
     4021 agtcagatac ggtagtaaca ttattgagaa cagtaatgtt gttaatgggt atatatttaa
     4081 ttattcgtgc gttgatttaa tacaacttta aaaggaggac gtcaatttga ggcttacaat
     4141 ttatcatacg aacgatattc atagtcattt acatgaatac gaacgcatta aagcatatat
     4201 ggcagaacat cggccacgac ttaatcatcc ttctttatat gttgatctag gtgatcatgt
     4261 agatttatcc gcacctataa ctgaagcaac tttaggtaaa aagaatgtgg cattactaaa
     4321 tgaagcaaaa tgtgatgttg caacaatcgg taataatgaa gggatgacca tttcacacga
     4381 agctttaaat cacctttacg acgaagcaaa atttatagtg acatgtagca atgttataga
     4441 tgaatcaggt catttaccaa ataatatcgt ttcttcttat attaaggaca tagacggtgt
     4501 gaaaatacta ttcgttgcag cgacagcacc ttttacccca ttttatcgtg cactaaattg
     4561 gattgttacc gatccacttg aatctataaa agaagaaatt gaacttcaac gaggtaaatt
     4621 tgatgtatta atcgtgctaa gtcattgtgg cattttcttc gatgaaacat tatgccaaga
     4681 attgcctgaa attgatgtca tttttggtag tcatacgcat cattattttg aacatggtga
     4741 aatcaataat ggtgtactga tggcggcagc tggaaagtat ggtaattatc ttggagaggt
     4801 taatttaact tttgaggcac ataaagtagt acataaaact gcaaagatta ttcctttaga
     4861 aacattacct gaagttgaaa cttcatttga agaagaagga aaaacgttaa tgtccaattc
     4921 agtaattcaa catccagtag tgcttaagcg tagtatgaat cacataactg aagctgcata
     4981 cttattagct caaagtgttt gtgagtatac acatgcacaa tgtgccatca tcaatgctgg
     5041 cttactcgtt aaagatattg taaaagatga agtgacagaa tatgacattc atcaaatgtt
     5101 accgcatccg attaatatgg taagggttag actttttggt gtgaaattaa aagagattat
     5161 agctaaaagt aataaacaag aatatatgta tgaacatgca caaggtttgg gtttcagagg
     5221 gaatatattt ggaggatata ttctttataa tttagggtac attcattcta cagggcgtta
     5281 ctatctgaat ggagaagaaa tcgaagacga caaagaatat gtactaggta cgatagatat
     5341 gtatacgttc ggtcgttatt tcccaacatt gaaagaatta ccaaaagagt atttaatgcc
     5401 agagttttta agagatatat ttaaagaaaa attattggaa tattaaaaag taagattatt
     5461 ggattttcat ttgtcatgaa tttcgatata atgtttaaag atacacttaa caggagggta
     5521 tgtgttgtta tggcgacaaa aaacgaggaa atattacgta aaccggattg gttgaaaata
     5581 aaattaaata ccaacgaaaa ctatacagga cttaagaaga tgatgaggga aaaaaatctt
     5641 aatactgtat gtgaagaagc taaatgtcct aatatacatg aatgttgggg tgcacgtcgt
     5701 acagcgacat ttatgatttt aggtgccgta tgtacaagag cttgtcgttt ttgtgcggtt
     5761 aagacaggtt tacctaatga acttgattta aatgagcctg aacgtgtagc tgaatcagtt
     5821 gaattaatga atttgaaaca cgttgttatc actgctgttg cgcgtgatga tttaagagat
     5881 gctggttcaa atgtttatgc tgagacagta cgtaaagtta gagaaagaaa tccatttaca
     5941 acgattgaaa ttttaccatc agatatgggc ggggactatg atgcgttaga aacattaatg
     6001 gcgtcaagac ctgacatttt aaaccataat attgaaactg ttcgtcgctt aacaccgaga
     6061 gttcgtgcgc gtgcgactta cgacagaaca ttagagtttt tacgtcgttc aaaagaatta
     6121 caaccggata tcccaactaa atcaagtatt atggttggat taggtgaaac tatagaagaa
     6181 atttatgaaa cgatggatga tttacgtgcg aatgatgtag atattttaac gattggtcaa
     6241 tatttacaac cttcacgtaa acatttaaag gttcaaaaat attacacgcc tttagagttt
     6301 ggtaaattaa gaaaagtggc aatggataaa gggtttaaac attgccaagc tggaccttta
     6361 gtacgtagtt cttatcatgc ggatgagcaa gtaaatgaag ctgctaaaga aaagcaacgc
     6421 caaggtgagg cacagttaaa tagttaatat ttaaccatta ataaggcata aaggcttagt
     6481 ttgtacaaaa cgaacgtgtc atagaagtaa tcattatttt tatgaaacac taaaagtata
     6541 aactgagcct ttcattgtca tagataggtg aagaatttga taaaagtaga tcaacattac
     6601 tttgaattaa tagaaaatta tcgcgaatgt tttaatgaag aacaatttat tgctaggtat
     6661 tcagatattt tagataaata tgattacata gttggtgact atggttacga tcaattacga
     6721 ttaaaaggtt tttacaaaga ttctaataaa aaagcagaga tgagtaaacg tttttcaaat
     6781 attcaagatt acatatttga atattgtaac tttggttgtc cttactttgt attaagacat
     6841 ttgtctaaac aagaggttaa aaagttaatc gaagaagttc atccgtctga tgtgatagat
     6901 gacgacaata aacttcaaga tgtgaagatt aagccaacca ttcaagatac tgaacattaa
     6961 taaaaccctt agctagattg aaaatgggaa tcatgcaatt caagcatgga cctgtaatct
     7021 agttaggggt ttttatcttt aatgaatgac ttcatttaaa tactcagtaa tttcatcgcc
     7081 ttcttcagca tttacaccta aaatatgagc gatatagcct tcttctttta aatcatcagt
     7141 accgataata ccgaatttat ttgtttgcat attaagtacg agtgtcttac cataatgtct
     7201 atttgtatgg actaacatca aatcatatcg actatgctcg ccaacaaaac caacaaactg
     7261 aacttgactc tcttcgttgt catcatataa atacatatca atcattttgt agcgactcct
     7321 tttaaaagta gtaaagttag tataacgaca aatgaagtat actgcaaaat tatgataata
     7381 tataagtgag aggtgacaag gaatgtattt tgtagacaaa gataaactaa ctcagaaatt
     7441 agcctattta caagcattaa ctgatgatta tcatgagagc aagcacaatc attatgcatt
     7501 tgaacgcatt gctcaaatgt tgatagaatc atcggtagat atagggaata tgattatcga
     7561 tgcatttatt ttaagggatc ctggtaatta taaagatgtg attgatatat tagaactaga
     7621 aaatgttatt actaaagaaa cacagcaggc gattaataaa actgtcggta ttcgtaaaca
     7681 atttacatat gattacacag ccttagatat taagattatc atgccaatgt ttgatgacgc
     7741 attaccttat tacaaacaat ttattacaga agtaacgaca tttttacatc aagaaaatgt
     7801 accagtaaca gcttttggta aaggagaaaa tcaataatga aacagtataa agcgtattta
     7861 atcgatttag atggcacaat gtatatggga acagatgaga ttgatggagc aaaacaattc
     7921 atcgattatt taaatgtaaa aggcattcct catttatacg taactaataa ttcaacaaaa
     7981 acacctgagc aagtaactga aaaattacgt gaaatgcaca ttgatgctaa accagaagag
     8041 gttgtaacgt cagcgttagc cactgctgat tatatttcag aacaatcacc aggagcatca
     8101 gtatatatgt taggtgggag tggtttaaat actgcgttaa ccgaagcggg acttgtcatt
     8161 aaaaatgacg agcatgttga ttatgtagtt attggacttg acgaacaagt tacatatgaa
     8221 aagcttgcga ttgcaacgtt aggtgtaaga aatggtgcaa catttatttc tacaaatcct
     8281 gatgtatcaa ttcctaaaga gcgtggttta ttacctggta atggtgctat tacaagtgtt
     8341 gtaagtgtat cgacaggtgt atcgccacaa tttattggta aaccagaacc gattattatg
     8401 gttaaagcat tagaaatttt aggattagat aaatccgaag ttgctatggt aggcgatttg
     8461 tacgataccg atattatgtc tggtattaac gtaggtatgg atacgattca tgtacaaaca
     8521 ggtgtatcta cgttagaaga tgtgcaaaat aaaaatgtgc caccaacgta ttcttttaaa
     8581 gatttaaatg aagcaatagc tgaattagaa aaatagatat agtcatttta taaagtaggt
     8641 gaattgattt ggtaaaaata gttgtttcga ggaaaattcc agataaattt tatcaacaat
     8701 taagtaaact tggtgacgtt gttatgtggc aaaaatcatt agtgcctatg cctaaagatc
     8761 aatttgtgac agcacttcgt gacgcagatg cttgttttat tacattaagt gaacagatcg
     8821 atgcagaaat tttagcgcaa tcaccaaatt taaaagtaat tgcgaatatg gctgtaggat
     8881 atgacaacat cgatgttgaa agtgcaacag cgaataacgt ggttgtcacg aatacaccaa
     8941 atgtacttac tgaaacaact gcagaattag gatttacatt aatgcttgct atagcacgcc
     9001 gtattgtaga agctgaaaaa tatgtagaag cagatgcatg gcaaagctgg ggtccttatt
     9061 tattgtcagg taaagatgtc ttcaattcaa ctattggaat atatggtatg ggagatattg
     9121 gtaaagcttt tgcaagaagg ttgcaagggt ttaatactaa tattctttat cataatcgat
     9181 caagacataa agatgcagag gcggacttta atgcaacata tgtttctttt gaaacgttgt
     9241 tagcagaaag tgattttatc atctgtacag cgccacttac aaaagaaaca catcataaat
     9301 ttaatgctga agcatttgaa caaatgaaaa atgatgcaat ttttattaat atcggtagag
     9361 gacaaattgt agatgaaaca gcattaatcg atgcactaga caataaagaa attttagcat
     9421 gtggtttaga tgtattagca aatgaaccga ttgatcatac acatccatta atgggacgtg
     9481 ataatgttct gattacacca cacattggta gcgcatcagt aacaacacgg gacaatatga
     9541 ttcaattatg tattaataat atagaagcgg ttatgacaaa tcaggtacca catactccag
     9601 taaattgaaa ataagtatgg tttttaatca ttggtaaaac aagcaaagca attgttaaat
     9661 atgttatgct tgacttgcga atgtatatat ttgcgctgat gataattcaa taacattata
     9721 aactcgtcat attaatgtga tgagtttatt tgattttgac gtatataaca aaattgttat
     9781 atagactgta attgtttatt gtttaaataa cctttaaaat gaatgatgtt ctcaacaaag
     9841 tgtatcattt acaataattg agtgatgaca accaattact atgttgaatc tataaaatta
     9901 aaattgaatt tcattatgaa tatcgatata atgaattgta aaaaacaaat tttaaaacgt
     9961 attttatatt gaacggtctt tataaaggcg tttttactaa gaactttaag aggtgcaata
    10021 tgaaatctaa aagtaaacag ccacctaata aatatgttga agcattcaaa ccatatttat
    10081 taacactatt gtatttggca atatttatta ctttatattt aatttatggc agtggcgaca
    10141 cacacaataa cttcatttat aatgagttct aatgagggag acttaatatg acagatatta
    10201 ttaacaagct gcaagcgttt gcggatgcaa atccacaaag cattgctgtt agacacacaa
    10261 ctgatgaatt aacttatcaa cagttaatgg atgagtctag taaattagca catcgattac
    10321 aaggtagtaa gaaaccgatg attttattcg gtcacatgtc accatatatg attgttggga
    10381 tgattggtgc cattaaagca ggatgtggat atgtacctgt agacacttca attcctgaag
    10441 accgtattaa aatgattatt aacaaggttc aaccagagtt tgtatttaat acgactgatg
    10501 aatcatttga aagtttagaa ggcgaagtat ttacaataga agatattaaa acatctcaag
    10561 acccagtaat ttttgatagt cagattaaag ataacgacac agtatacaca atctttacat
    10621 ctggttctac tggggaacct aaaggtgttc aaattgaata tgcaagttta gttcaattta
    10681 ctgagtggat gttagaactt aataaatcag gaaatgaaca acaatggctt aaccaagcgc
    10741 cattttcatt tgatttatct gtaatggcta tttatccatg tttagcatca ggcggtacat
    10801 taaatcttgt agataaaaac atgattaata aacctaaatt attaaatgaa atgctaacag
    10861 caacaccgat taacatttgg gtatcaacac catcatttat ggaaatgtgt ttattattac
    10921 caacgcttaa tgaagaacaa tatggtagtc ttaacgaatt cttcttctgt ggtgaaattc
    10981 tacctcacag agcagcaaaa gcgttagtaa accgtttccc aagtgcgacg atttacaaca
    11041 catatggtcc aactgaagct acggtagcag ttacaagtat tcaaattaca caagaaatct
    11101 tagatcaata tccgacatta cctgttggcg ttgaaagacc aggcgcaaga ttatctacta
    11161 cagatgaagg tgaacttgtt atcgaaggtc aaagtgtaag tttaggatac ttaaaaaatg
    11221 accaaaaaac agctgaagta tttaatttcg atgacggtat tcgtacatat cacactggtg
    11281 ataaagcgaa gtttgaaaat ggtcaatggt tcattcaagg tcgtattgat ttccaaatca
    11341 aattgaatgg ctacagaatg gaattagaag aaattgaaac acaattacgc cagtctgagt
    11401 tcgtaaaaga agcgattgtt gtacctgtat ataaaaatga taaagttatt catttaattg
    11461 gtgcaattgt gccaacgact gaagttacgg acaatgcaga aatgactaaa aatattaaaa
    11521 atgacttgaa atcacgctta ccagagtata tgattcctag aaagtttgaa tggatggaac
    11581 aattgccatt gacttcaaat ggtaaaattg acagaaagaa aattgcagag gtaattaacg
    11641 gatgattcca tatggtgatt ttacattctt cttaattgct ttaattgcat tattaccagt
    11701 cattatactt ggatttttag gtaagcgaag ttacatttat aatggcgtag ttacagcatt
    11761 tatgattgtg ttaatctttt cttctgataa acataatctg tttgaccaaa agtatttaag
    11821 tgttcaatta attagtttta ttatttacgt cgtatggcaa gttttattga taatgtttta
    11881 ttatcattca aaaccaaaaa ataattcatt ttcaaaattt gtaactgtaa tggttttatc
    11941 aatattgcca ttagcacttg tgaaagtgtt acaaagtaca tggttaggtg gacatcagat
    12001 tcacttccat gaaagtaaat taattgaatt tgttggtttc ttaggaattt cttatgttac
    12061 attcaaaagt gtgcagttaa ttatggaaat tcgtgatggt tctatcaaag aaattaaagt
    12121 atggaaatta attcaattta tttcattctt cccaacgatt tcatctggac caatcgatcg
    12181 ttacaaacgt ttcgttaaag acgataaaaa agtaccaaca ggcaatgaat atcgtgaatt
    12241 agtattaaaa gcaattcaca tgattatgct tggtttcttg tataaatata ttgttgctta
    12301 ctttattaac acatatgcaa tcatgccgtt acaattagac ttacatggct ttgtcaattt
    12361 gtggttatat atgtacgcat acagcttata tttattcttt gactttgcag gttatagttt
    12421 atttgcgata gcatttagtt atttattcgg tattaaaaca ccaccaaact tcgataaacc
    12481 tttcaaagcg aaaaatatta aagatttctg gaatagatgg catatgacat tatcattctg
    12541 gttcagagat tgtatttaca tgagatcttt attctacatg tctcgtaaaa aattattgaa
    12601 gagtcaattt gcaatgtcta acgtggcatt cttaatcaac ttcttcataa tgggaatttg
    12661 gcatggtatc gaagtgtatt acattgttta tggtttatac catgcagcat tgtttatagg
    12721 ttatggctat tatgaacgtt ggcgtaagaa acatccgcca cgttggcaaa atggtttcac
    12781 aacagcactt agcattgtga ttacattcca ctttgtaaca tttggctttt taatcttctc
    12841 aggtaaactt atataataaa ggagaattta attatggaat ttagagaaca agtattaaat
    12901 ttattagcag aagtagcaga aaatgatatt gtaaaagaaa atccagacgt agaaattttt
    12961 gaagaaggta ttattgattc tttccaaaca gttggattat tattagagat tcaaaataaa
    13021 cttgatatcg aagtatctat tatggacttt gatagagatg agtgggcaac accaaataaa
    13081 atcgttgaag cattagaaga gttacgatga aattaaaacc ttttttaccc attttaatta
    13141 gtggagcggt attcattgtc tttctattat tacctgctag ttggtttaca ggattagtaa
    13201 atgaaaagac tgtagaagat aatagaactt cattgacaga tcaagtacta aaaggcacac
    13261 tcattcaaga taagttatac gaatcaaaca agtattatcc tatatacggc tctagtgaat
    13321 taggtaaaga tgacccattt aatcctgcaa ttgcattaaa taagcataac gccaacaaaa
    13381 aagcattctt attaggtgct ggtggttcta cagacttaat taacgcagtt gaacttgcat
    13441 cacagtatga taaattaaaa ggtaagaaat taacatttat tatttcacca caatggttta
    13501 caaaccatgg tttaacgaat caaaactttg atgctcgtat gtctcaaact caaattaatc
    13561 aaatgttcca gcagaaaaac atgtctactg aattaaaacg tcgttatgca caacgtttat
    13621 tacagtttcc acatgtacac aataaagaat acttgaaatc ttatgctaaa aaccctaaag
    13681 aaactaaaga tagttatatt tctggtttta aagagaatca attgattaaa atagaagcga
    13741 ttaaatcatt gtttgcaatg gataaatctc cattagaaca tgttaaacct gctacaaaac
    13801 cagacgcttc ttgggatgag atgaaacaaa aagcagttga aattggtaaa gctgatacta
    13861 catcgaataa atttggtatt agagatcaat actggaaatt aattcaagaa agtaagcgta
    13921 aagttagacg tgactacgaa ttcaatgtta attctccaga attccaagat ttagaattac
    13981 ttgtaaaaac aatgcgtgct gctggtgcag atgttcaata tgtaagtatt ccatcaaacg
    14041 gtgtatggta tgaccacatt ggtatcgata aagaacgtcg tcaagcagtt tataaaaaaa
    14101 tccattctac tgttgtagat aatggtggta aaatttacga tatgactgat aaagaatatg
    14161 aaaaatatgt tatcagtgat gccgtacaca tcggttggaa aggttgggtt tatatggatg
    14221 agcaaattgc gaaacatatg aaaggtgaac cacaacctga agtagataaa cctaaaaatt
    14281 aaaatacaaa tagcacataa ctcaacgatt ttgattgagc gtatgtgcta tttttatatt
    14341 ttaaatttca tagaatagaa tagtaatatg tgcttggata tg
//
LOCUS       DAKTLH010000061        14322 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.061, whole genome shotgun sequence.
ACCESSION   DAKTLH010000061 DAKTLH010000000
VERSION     DAKTLH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14322
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.061"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(279..785)
                     /locus_tag="O0394_001510"
     CDS             complement(279..785)
                     /locus_tag="O0394_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499590.1"
                     /translation="MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            complement(921..1721)
                     /locus_tag="O0394_001511"
     CDS             complement(921..1721)
                     /locus_tag="O0394_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0499591.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAELA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLTLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTAAFINKHHDEKHV"
     gene            complement(1705..2424)
                     /locus_tag="O0394_001512"
     CDS             complement(1705..2424)
                     /locus_tag="O0394_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0499592.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            complement(2479..3429)
                     /locus_tag="O0394_001513"
     CDS             complement(2479..3429)
                     /locus_tag="O0394_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0499593.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVTGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            complement(3577..4464)
                     /locus_tag="O0394_001514"
     CDS             complement(3577..4464)
                     /locus_tag="O0394_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0499594.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSNDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKKGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            complement(4843..5478)
                     /locus_tag="O0394_001515"
     CDS             complement(4843..5478)
                     /locus_tag="O0394_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HCY0499595.1"
                     /translation="MLGTDELYKVLYEHLGPQFWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIHSSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKN
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            complement(5866..7527)
                     /locus_tag="O0394_001516"
     CDS             complement(5866..7527)
                     /locus_tag="O0394_001516"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCY0499596.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(7524..7940)
                     /locus_tag="O0394_001517"
     CDS             complement(7524..7940)
                     /locus_tag="O0394_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCY0499597.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            complement(8033..8140)
                     /locus_tag="O0394_001518"
     CDS             complement(8033..8140)
                     /locus_tag="O0394_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499598.1"
                     /translation="MKDSENLVFFGFEVLFVFYKMAYIYEALIKYGIVN"
     gene            complement(8215..9225)
                     /gene="adhP"
                     /locus_tag="O0394_001519"
     CDS             complement(8215..9225)
                     /gene="adhP"
                     /locus_tag="O0394_001519"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCY0499599.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
     gene            complement(9724..10245)
                     /locus_tag="O0394_001520"
     CDS             complement(9724..10245)
                     /locus_tag="O0394_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HCY0499600.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(10273..11568)
                     /locus_tag="O0394_001521"
     CDS             complement(10273..11568)
                     /locus_tag="O0394_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCY0499601.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVAPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(11608..12084)
                     /locus_tag="O0394_001522"
     CDS             complement(11608..12084)
                     /locus_tag="O0394_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0499602.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            complement(12253..12789)
                     /locus_tag="O0394_001523"
     CDS             complement(12253..12789)
                     /locus_tag="O0394_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCY0499603.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            complement(13000..13938)
                     /locus_tag="O0394_001524"
     CDS             complement(13000..13938)
                     /locus_tag="O0394_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0499604.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDNNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVVENIKAADIVLSDDEIQYIDELFPIED"
     gene            complement(14208..>14322)
                     /locus_tag="O0394_001525"
     CDS             complement(14208..>14322)
                     /locus_tag="O0394_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0499605.1"
                     /translation="YQNIIVYFVWIAVIMGFFLVNIALIIDKNIHVILKNQ"
BASE COUNT         4512 a         2725 c         1859 g         5226 t
ORIGIN      
        1 attttttata tcagtaatta ctttctcttt aatatcatca atcaaatgcg gtgataaacc
       61 tttatgatta aagtaatctt ctatattttc tagtaaaatt ttatcgttaa tacgcattgg
      121 tagtactcct ttaaaatgaa acatctttat ttattgtatt ggtttaattt ccccttcgca
      181 aatcataaaa attaatattc actgtattat gccatgtaat aaaataaccg acaagtattg
      241 cgatttaact tgtcggttaa gcacgattaa tttaattatt atttttccaa atcaatacga
      301 tataatttta tgtttttatc ttttggtgct gaggcaaatt gataagtcgt tttgcctttt
      361 tcaatatatc caaaattacc agcaactttg ctatattccg tatgtgttac tgctttgcca
      421 acataatttt taacagcttg atatgttgga ccattagcat cattatacat cattgaaata
      481 tgagttactt taccattgtt tttcttacca tcagtagtta ctaataacat acctttttta
      541 gtatggaatt cgtaataatg ttcagttcca tcttcattgt aagaatacat cggatttttg
      601 tacttagtta aaacatcttt aatagattcg ccaattttca cattttctaa tgactgatca
      661 cccttaatta attgtttaac agtatcaata gagttgccac ttgctgcttc ggcactagtg
      721 ttcactaagc ctactcccat tacaacagaa catgcaatta tacttgccgt tagtagcttt
      781 ttcataagca ctctctcctt ttatttatat cgtcttgtgc tcacaaccat tatacaacaa
      841 tcatttctaa aacaacagtc atattacaat tatattacaa ataataatta ctacttttat
      901 atttcactta tcactaaaaa ttaaacatgc ttttcatcgt gatgtttgtt gataaatgca
      961 gctgtatctt cgacgaatct ttcttgctct tcaacaaatg ggaatgcact tgattcttga
     1021 tatacttcaa agtccgcatt ttggattaaa tcagctactt ctttagcttc taatcttgtt
     1081 gttctttcac cgaattctcc tgcaataatt tttgtcggta ctacgacatt tctataagtt
     1141 tgagaaatat cagcgttttt gaatacctct ttaactgctt gaatttcttc tttagttgat
     1201 atttcatttg tatctacgac atgtttgagg aatcgattca tttttctcgg acgataatac
     1261 ttacgcttat ttaaaaattt atcttgtttt tcaggatccc agtttcgaat aatatgggca
     1321 tattttctaa ataaacgttc ttccggtaat tcaccttcaa tagatgttgg atttaccaac
     1381 gtaagtgatg atgtaaattc aggataacgt actgaaatat ccgcaccaat gattccaccc
     1441 atttcatggc atacaaatgc aacttcttca atatataaat atttaagtaa ttcaacaatg
     1501 tcatcagaaa aatcttttat ttcaatgtga cgaggtttat cagaatagcc atgtccacgt
     1561 aaatcaatta acacaacttg aaatgatttt gctaattctg ctgctaattt attaaacaca
     1621 gaataattat caagtacagt atgaatcaat acgataggat agccttcacc taatgtactg
     1681 taatgtatcg atgttccatc ttttctagta aatagatcca taattttctc ctttattatc
     1741 attttttatt ggtaaaatgg ttaataattc ttccactgtt gtaattgtaa aatcaacttc
     1801 ttcttcgagt ggttcaattt cagcatcttc ttgtttatac catacactaa ccatgcccat
     1861 agctcgtgct ggagctacat catttaacgc atcatcgcca acatacataa tttgctcagg
     1921 taatacccct agttgatcaa tcatatcttc aaaaatttta ggatgtggtt tacgaaaacc
     1981 aactgtttct gatgttgata aataattaat aacatgcatc aaaccaagtg aatgtaatcg
     2041 aaattgttta atcttagatt taccatttgc gataacacca atttgaaagc ctttttgcga
     2101 tagcttttct agtgtataca aagtatcgta ataaggaaat acataacgat aaaaatgcat
     2161 ttcaaaatca ttaaataagt ctttccaagt taacctatct acatggaaac gtttaatgat
     2221 ttctttatat aaatcaggtt tatcattatc ttcatcgtca tctagctcaa taaatgcttt
     2281 tttaaaatct gccagttgaa catgaattaa gtagtcatga aatcgttcat attgctcttc
     2341 gataaatttc tctcgagatt taactctgtc caacaacgta ccttctaaat caaatacaac
     2401 tgctttaatt tgattcaaat ccatcgctta tgccctcttt atgtttatag ttatttttat
     2461 tttagcaaat gtcgtgtgct agtgtaaccg tttcgactga gatattagca tgtaaaataa
     2521 cactggtata ccaattaatg caatgataat acttgccggt atttgtactg gtgacaatat
     2581 atggctacca ataaaatctg ccataaccat tatttgtcct ccagttacaa tatttaaagc
     2641 caattgtctt cctaatgatt gtttccactg ccattttcta atgagttgcg gtatcaccat
     2701 accaatgaat ccaatgatgc caacatatgc gacatttatc gccgttatca tagaagctat
     2761 acataacgcg atatatgtaa tcaattgaac atttaagcct agtgactgac tttttagttc
     2821 tcctaattgc aataacttta gttgattaag aatgataaat ataccaaaca atgcaatgat
     2881 aaatgtgatt gctattatag atacatttga gtattctgca tcaccaaaac caccaaacag
     2941 ataattggca attgtattta atttgcgagg ttttaataaa atcaaaaaat atagaagtga
     3001 attgaataac gcaccaatca ttaaaccact taatattaag attctaactg gatagccttg
     3061 gctcaatacc gacgtaatga ctaatacagt tattaatgtt atcaaactaa atgttattga
     3121 aaatacagga atccataacg ttgttaaacc taaaaataat gctaatcctg aaccaaatgt
     3181 agcgccgctt gccaatccta atgtaaagct atctgccaat gcattattta aaactgtttg
     3241 aaatataagg cctgcaacag ttaatatcat acctgtcaac aatgcttcta ataatctcgg
     3301 taatctaaca ttgattaaaa tagactggtt aaatacatca ttgatatcgc ccaactgcca
     3361 aaatagatat atgctagttg ttataatcaa tatgactatc cagctcaata atactttatt
     3421 aaatgtcata gcattctctt ttctttcatc gataattaca ttcattatag caatcacata
     3481 ctcgcgttgc tatcattcac taaatatatt ttaaaaaccc cttatctcaa acatgtattt
     3541 tttacatgta gcaataaggg gcataattag aatggtttat tttctataaa ttgcatctct
     3601 taattctttt aatccttcat caatacgtgg acctggtctt gatacttcat caccatttac
     3661 aacttcaata cgtgtattct tgacagcatt aattttattg aatccacctc tttttttgat
     3721 gatatccata taatctgatc ttgtcttagc ttccgtcgaa attaatatat ctgggttctt
     3781 tttaataata ctttccttcg ttacagggtt ccaaccatta atgtcactat acacattttg
     3841 ggcttctaat ttttctaaca tatcattaaa aaatgtatgc ttccctgctg tatatatttc
     3901 aggctttgat gaaacctcaa taaatacttt tgatttttta tgatgagcag gaattgaatc
     3961 tatgacttta tcgatattat ctttagtttc ctcaacaagt tgttcagcct gcttatcatg
     4021 atgcgttaat ttcccaattt gcttaaatgt gttgtaagtt tcatcaattg attgtgcatc
     4081 tttaacatac actactttga tgccttgttt ctctaatgat gacaatactt tattagcagt
     4141 tgccttttgc gactcatgcg caagaattag atctggcttt gcctttaaaa gttcctcttt
     4201 atttagattc aaagcatcaa attgtttctt accctttttc acatcttttg gataatcatc
     4261 aaccgttgaa acaccaacta tgtatttacc taatcctaat tcatataaaa tttcagtatt
     4321 actaggcatt aacgaaacaa ttctatggta cggcgattta gacgagccat tatcattgct
     4381 accttgttta tcattatctt tcataccaca gccactcagg acaagcataa aaatcaaaat
     4441 aaaagcaatt aacgatttct tcacactatc acatctcctt tttaatccca cttttcgctt
     4501 tagcgattag agggatttat gcagttgtgc ggtagcatcc aactgcgttc ctcttcatct
     4561 ttagcgatta gaggctttta tgcagttgtg ccttagcatc caactgcgtt cctcttcacc
     4621 tttagtgatt agaggctttt atgcagttgc tgcacaaaca actgtattcc tttactcccc
     4681 ttgagcttta gcgattaggg gctcttatgc agttgctgca caaacaaaca actgtttttt
     4741 tctttatatg cttctattta ttatataagt ccacatacta aaagaaaata gtaaattatt
     4801 ttttatattt catataaact catctatcta acttcacagc gtttactttt taaagtaagg
     4861 ttctaaaaaa tcataattct ttatatcttt gtctctaaag taatgtttac caaatacatc
     4921 taacagagca tgaaattcat tagcatcttg atttgtaaaa tgatttggta atgtgactac
     4981 tttttttaat tgatcataat ttttagtgtt ttcatatcct aatttatcat atatttttct
     5041 tgtatagcta tctggaataa attcaatacg tccgaatata taaacaagta agacatctgc
     5101 tgtttcactt ccaatacctt tcaaagataa taattctttt cttaatccac ctttatatcg
     5161 ctcattaatc tcttgataat tgaaatgatg tcgtgctaac catgttaata atgttttaat
     5221 cgtcagtgat ttacttttat aaaagccact tgaatgtatc aatgattgta acgtttcaat
     5281 aggtagttct aatatatgat ttggattaaa atgcgtatgt tctttaatct gattcaatgc
     5341 aatttctgca tttcgccatc tagtattttg aactaaaatt gcacctaaca tcatttcaat
     5401 gtcattatca gcaggccacc aaaattgtgg tccgagatgt tcatataaaa ctttatataa
     5461 ttcatcagtt cctaacattt acaccctcca ttttagaggg agtgggacag aaatgatatt
     5521 atcgtaaaat ttatttcgtc gtcccacccc aacttgcatt gtctgtagaa attgggaatc
     5581 caatttctct ttgttggggc cccgccggca aggttgacta gaattgaaaa aagcttgtta
     5641 caagcgaatt ttcgttcagt caactactgc cattataact ttgtagagca tagaacattg
     5701 atttatgtct caggctctct tatgcagttg tgcagagcat ccaactgtgt tcctcttaac
     5761 cttattcaca tactaagcat tccccaatat gtcacatacc taaaaaaaca ctccaacaca
     5821 aagtatcttt cgagaaactc tacgttggag tgtatataaa tgttcttaca ttgattcagg
     5881 tgcgcttaca ccgaccattg ccaatgcatt tttcaatgta attctgaccg cttcaatcat
     5941 agcaacatgt gcttttgttt tttcaatatc atctgttaac actttttcag cattatagaa
     6001 tttatggaaa tgagaagcta aatcttgaat ataattagta attctatgtg ccgatctatg
     6061 ctcagcagca ctttcaattg taggttcgaa atcagctact tttttcaaca attcaatcgc
     6121 tttttcatta gtaatcgttg taaaatcatt cgcagcagtc acttcaatac cttgctcttt
     6181 cgcttgtttt aaaattgaac aaatacgcgc atgtgcatat tgagcatagt aaactggatt
     6241 gtcttgagat tgctctttcg ctaattccat atcaaaatca aagtgactat caggactacg
     6301 catagttaag aaataacgtg cagcgtcaac gccaacttcg tccataattt ctcttaatgt
     6361 aatcgcatta ccagtacgtt tactcatctt cacttcttta ccattttcca ttaaacgaac
     6421 catttgcatg atttgaattt ctaaacgatt actatctaca ccaaacgttt caagagatgc
     6481 tttcaaacga ttaatataac catgatgatc agcaccaaat aaatcgatta aaatgtcatt
     6541 accacgtttt actttatcga agtggtacgc aatatctggt aagaaatacg tatatgtacc
     6601 gtcattttta attaatactc tgtctttgtc gtctttaaaa tcagttgtac gtaaccatgt
     6661 agcgccatca gcttcatacg tataacctaa ttctttcatt tttgctaaaa cttcaagaat
     6721 ttctcctttt tcatataaag atgtttcact aaaccaatta tcaaaatgcg tattgaactc
     6781 tgctaaatca tttttcaatt tagccatttc gtattctacg cctaattttc taaattcttt
     6841 caaacgtgct tcttcagaat aatctttaat ttcagggtgt ttctctgcta aatctttacc
     6901 tatttcaata atatcttttc cattatagcc atcttctggc attgaataac tattgtcacc
     6961 taaagcttca aagaaacgtg tttcaatcga acgcgctaag ttagtaattt gattaccagc
     7021 atcattaata taatattcac gtgttacatt atagccagct gcagttaaaa tattagctaa
     7081 agcatcacca actgctgcat ttctagcatg accaatatgt aaatctcctg tagggttagc
     7141 tgaaacatac tcaagcaata cattttgacc ttttgattca tttacatgtc caaattgatc
     7201 acctttttca attgcttcag gaataattgc tgttaaatac tgattatcta agtaaaaatt
     7261 aatgaatcct ggaccagcaa tgtcaatttg ttttacatgt gctttttcag tatctaagtt
     7321 atcaacaatc gcttgagcaa tttcacgagg attacgcttt gcaatcttag tcagtaccat
     7381 cgcaatatta gtagcataat ctccattttt tgtatcttta ggaacttcaa ttttaatatc
     7441 aggaatctca tctgctaatc ctgctttgtt aatacttgct gcaatttctt ctactaatgt
     7501 ttgtttcact tgatcaataa tattcattcg cctatctcct tatagttaat ttcatattga
     7561 taagaaccca ttttttcatt atcttgatat aattcataat gaatctttag cttgccaccg
     7621 tctccactta cgaaatgtaa aatgcgtaat gttttaactt ctagtggaat tcgtccagcc
     7681 gatatatcgt aaaaagttgt tgtcgtttgt ccttcaacaa aatgcaaatt catattaatg
     7741 tcgcctttac gaatcaactt aacgctatca tcatccactt ttattgtaac attaactgtt
     7801 gcatcctcaa tttgttctac ataccgaata aagttagatt gcctttgttg ccaagttcct
     7861 tcagtagtaa attcaaattt ttctttttcg ttgtgctgtt tcaacacttg ctttgtttga
     7921 atacttactt ttttatccaa tttactactc accttaatct tcgcataata gaaatattat
     7981 agcataaaat aaggtcatct tgttatgcct tcttaaagct aaataggttc aattaattaa
     8041 caattccata cttaatcaac gcttcatata tgtaagccat tttataaaat acaaacaaca
     8101 cctcaaaacc aaaaaacacg agattttcac tatctttcat gaaaacctcg tgtttgatta
     8161 taatgatagt gttcagagca ctaccttctt tatctatata gtcagttgat atttttaaaa
     8221 tttaataacc attctaccag ttatagtacc attttccatt tcttcaaaaa tatcattgat
     8281 ttcttctaat tttcttaatt gaactttagg tgttacttta ttttcagcag caaattcaaa
     8341 cgcttcacgt aagtcttgtc ttgtaccaac aagtgaacct actacttcaa taccatcaag
     8401 cactaatctt gggatatcta agttcatttt atcaacaggt aaaccaacgg caacaactct
     8461 tgcaccagct tttacaacat caaccgcttg gttaaatggc gtcttagcaa ctgaagttac
     8521 cactgttgca tctaatcctt tgttatcagt taatttcata acttctgcaa ctggatcaac
     8581 atctttagaa ttaataatag catcagcacc taattctttc gcgaatgcta atttatcatc
     8641 attgatgtcg aaggcaacaa ttttagcccc cataacgttt ttagcatatt gtaaagctag
     8701 gttacctaaa ccacctatac caaaaacacc taaccattgt ccaggtttta cattacttac
     8761 ttttacagct ttataagttg tcacacctgc gcatgtaata gaagacgctg ctgctggatc
     8821 taatttttca ggtactttca cagcatagtc tgcagtaaca ataacttgtt cagccattgc
     8881 accatctact gtataaccag catttttcac actacggcaa agtgtttcac gacctgttgt
     8941 acaatattca catcttccac agctttcgaa catccaagcg atagacacac ggtctccaat
     9001 ttttaatgat tctacatctt ccgcaacttc gatgacttta ccaatacctt catgacctaa
     9061 agtaacgcct gtaacatcac caaaatcagc attcttaaca tgtaaatcgg tatgacaaac
     9121 gccacaatat tccgtttgta ccaacgcttc accaggtttt aaagctctta actttttgtc
     9181 ctcaatactt actttgtgat ctttcgttac aactgctgct ctcataataa tgtcctcctt
     9241 atagtcactc cagtatgtat gatattaatt ggctattact ttcaaaaaag aaaacgaata
     9301 attatagatg taatttttat ttgtaataga cgccagatta aaacatacta ctgtgacctt
     9361 gtttttagtt agatatcaac cgttaacttt taacgtttcc ctagttaata tgttaaaaga
     9421 aatgacataa tcgatatgct aacgtgtaat ttttacttca catatattta tttaaagata
     9481 atttataata ctcttatctt cactcttatt ataatgtgaa ttaattcaca aagtatatag
     9541 ctatgatcaa aaaaatgtgt cagttattca aattttttat taactacaga ttaatttaat
     9601 ttcaaaaaaa ttaaaaaaac tcccaatcaa tctaagacat tattttcacc tcacgattta
     9661 cgtcgtcatt gagaaatgaa tcatatcttt atagattaat tgggaaattt tagtttctaa
     9721 tatttaatta aatgcttgat gtaaagcatc agaagaatgc ttccacatac cttcatcatg
     9781 gttcattaaa aagtctttta aaatagcttt attctcgtca ccaatttgat ctatgataat
     9841 atgatgtttc attgatttat ccatcatatt aacatgttca ggcatacttt tataaccacg
     9901 acgaattgct ttattcacta ataaatagca cgctgttaaa ccagcgtaat atggtccttg
     9961 ttcattacgt tctgttgtta cccaacacaa ccaatattct ttagcttcat cgccttcaac
    10021 agcagcttta tcagtaatcc atttcacacc tttttcaact ttggcacgag catgcatgcc
    10081 tccaatatca ataaatgctt ctttgttagc aacatcgata aaaactggtg caatattgtc
    10141 taagctaatt gaaccaatat ttgtaccttt atgaccatct agagggtcat tttttatgat
    10201 attaaaatta aagccttttt tctcagacaa tgatataacc tccatattta acgataacaa
    10261 ttttagccct gtttaattaa gctcattaat caaattttca atagcttctc taattgtctt
    10321 atctttaatc tttgcaacca tttctctagg ataatataat ttatagtctt ggcgattaat
    10381 gcctgtaata ctatgaatga ctaatgattg attgcttatt tctctaatcg taccatcttg
    10441 tcttaataaa tgaatcggtt tgcgatttga ccccggtcga tcatagtcat atggcaaatc
    10501 agaaaatgct tcactcacaa aataataatc tggattaata ccacctgctt caaacagttc
    10561 ttgcagttct gatattgtaa taattgagcc atcaaatgga atatatttaa ataagtctcg
    10621 attaataaat cgacttgcta aatcacttaa aatagcatca tcttcttttg tccatttttc
    10681 caaataatac gtaactaccg cttcatcgag ttcaacatat tgttcaatcg taactgtctc
    10741 ttcaaaaaat ggaataaaat catgtggatg caacttaaat tcatagcctt cattataaag
    10801 ctgttttgcg cgtttcaaac aattattaag cagcacttct ccaccacgac taactgggtg
    10861 gaagtaaatt tgccaataca tttgataacg actcataata aagttttcaa ctgcatgcat
    10921 accactttct ttgattagta cttcatcttt agaaggtcgc attaatctta aaatacgctc
    10981 catatcaaaa gcaccatatg atacacctgt gaaatacgca tcacgttgta aataatccat
    11041 tctatccgca tcgatttgtg acgaaatcat cgaaatgacc aatttattat gatgcgtttt
    11101 attaattact tccgcaactt ctcttggaaa ctcaggcgcc actttacgta atacagcatt
    11161 cacctcagta tctccagtaa taatcgcttg tgtgtatgct tcatggtctg tattaaatat
    11221 tttttcaaaa ctatgtgaaa atggaccatg tcctaaatca tgtaataatg cagcacataa
    11281 tgccaacggt ctatctttat tgtcccatgc atcatgacca ataaatgact catcaattaa
    11341 tcgtctaact atttcataca cacctaaaga atgtccaaag cgactatgtt ctgctgtgtg
    11401 aaaagatagg tacagtgttc ctagttgtct aattcgacgt aacctttgga attccttagt
    11461 tttaattaaa tcccatatca attgatcttc aacatgaata tatcgatgta tcgggtcttt
    11521 aaaaactttt tcttctacta attttaaatc tacatatgcg ttagtcatta ttcccctcct
    11581 tttcgtttaa tataatattt aatttactta aaatgctttg tacataagtg ctaagtctaa
    11641 cttttcgcca tacatttctg gctcataaga gcgtaagatt gtaaaacctt gctctttata
    11701 gtaagctact gcttcttcat ttttattatc tacttctaag taaacacctt caaatttatc
    11761 ttcaaaacgt gataatcctt catttaacaa tgctgtacca taacctgtat gttgcgattc
    11821 tggtttaaca taatgagctg ataaatataa ttcttcaccg taaataaagt tagcaaagcc
    11881 aacgatgtca ttaccttctt caacgactaa gaataattgt tcttgaagtc ttttctttaa
    11941 atgatgttca ttatatgaag cttctaacaa gtgattaact gttgtcgcag cgtatatatt
    12001 taagtatgta ttaaaccaag ctttagttgc gacatctcta atttgaacaa catctttttc
    12061 agttgcttgt cttaccttga acatgacttt ctccccttat taacaagttt taataacggc
    12121 attataccac aacttgctca atacttaata aacaatgatt gtctattcaa tttatatatt
    12181 tatattttcc gttaaaatta aaaataaaaa ataacgaagc aaaaaatcac ttcgtttagt
    12241 atgaggtatg tcttattgca atatactatt ccactcagtt gcacgtgcta aggcatagtt
    12301 gtctttcatg atgtcaccag gcttttcagc agttccaata atataaccat ttaaagtggc
    12361 acctaaaaag tctaaactat atttcatttg cgtaattgct ggcttcgctt ttatttttgg
    12421 acaatctcca ccaactaaaa taactctaaa atccttttcg gccatttgtg ccttaaaatt
    12481 aggatatcgt ttatcttgta atgtttctga ccaatgttcg ataaatgctt tcaatggtgc
    12541 tgaaatgcta taccaataca ctggtgatgc aaaaataatt gtatcactag ccaatatttt
    12601 atctagaatc ggcaaatagt catcgtcata tgaagtaata gtctctgctg tatgtctcac
    12661 gtcacgtatc ggtttaaact gatgttgtgt cacgtcaatc cattgatact ctaaatcttg
    12721 caaagcgaat tttgttaatt gtgcagtatt accgtttggt ctactcccac caaacaaaac
    12781 agtaatcatt ttagcctaac ctcacttttg attaataaat atctgtgttt ttcgttacct
    12841 aattatacta tcataagctt tgcctaccga atagtaaaac gcttacaact tttatataaa
    12901 tttgacgaaa tttcgtcatg ccttatataa cgtcgtttgt gatacggggc taattcatga
    12961 tgaaattaga tacatatatc accattaaat acaattcatt tagtcttcaa tcggaaacag
    13021 ttcatcgata tattgaatct catcatctga taaaacgata tctgcagctt taatattttc
    13081 aacgacttgt tctgcacgtt ttgcaccagg aataatcaca tcgatagctg gtctcgttaa
    13141 ataaaatgct aatacaatgt tcgcaattga agtttgatgt gctgcagcta tgctttccaa
    13201 agcttttacg cgacgcacat tttcttcaaa tacacctggt ttaaaatcac gacgtgtagt
    13261 acgatggtca ctaaatttag tgttctcatc atattttcca gctaaaatac cggatgctaa
    13321 tgggaaatat ggaataaatg tgatttggtg atcaacacaa tattgtaata ctgcctcatt
    13381 ttcgcgatgc aataaattat attctaactg tacaacatca acgtaaccat ctttatttgc
    13441 ttctttaagt tgatctaatg tgaaatttga tacaccaatt gctttaatct tcccttgttc
    13501 cttaagctct tgtaatgctg caactgcttg atctttcgga gtgttgttat ccggaaaatg
    13561 aatataatat aaatcgatat aatcagtttg tagacgtttc aaactattct caacttgttg
    13621 ttttaaatat tccggttgat tgttctgatg tacttcttga ttttcatcaa attcatgaga
    13681 ccctttcgta gcaattttaa tttgctctcg cggatattct ttaacaactt ctccaaccaa
    13741 ttcttctgat cgttctggcc cataaatata tgccgtatct aataaattaa taccatgatt
    13801 aatggcttga cgaacaacat cttttccttg ttcttcatct aagttcggat ataaattatg
    13861 cccacctact gcgttcgtcc caagtgcgat tggaaacact tcaacatcag atttacctaa
    13921 gtttacaaat tgcttcatta gacccagctc cttatcttta taacttttcc actatcacac
    13981 tatcatgatt taatacaact tccaaatgtt agaattagag cttgaattta agcggtgtaa
    14041 taggaaaacg ttatataaaa ctcagttaaa ataagtattg cttaaattga tatgcatata
    14101 gcgcgattgc tcgttgacct ttaaattaca tgtacttttg aatcagaaat tgaaaaacat
    14161 ttaagaacga caatattaaa gccattgcaa atttaatttg gtaatttcta ttggttttta
    14221 agtatgacat gaatgttttt atctataatt aaagctatat taactagaaa aaaacccatt
    14281 ataactgcaa tccaaacaaa atatactatt atgttttgat aa
//
LOCUS       DAKTLH010000062        14296 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.062, whole genome shotgun sequence.
ACCESSION   DAKTLH010000062 DAKTLH010000000
VERSION     DAKTLH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14296
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.062"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(120..269)
                     /gene="rpmG"
                     /locus_tag="O0394_001526"
     CDS             complement(120..269)
                     /gene="rpmG"
                     /locus_tag="O0394_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCY0499606.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            complement(360..1877)
                     /locus_tag="O0394_001527"
     CDS             complement(360..1877)
                     /locus_tag="O0394_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HCY0499607.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            2081..3535
                     /locus_tag="O0394_001528"
     CDS             2081..3535
                     /locus_tag="O0394_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0499608.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWLLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
     gene            3674..3778
                     /locus_tag="O0394_001529"
     CDS             3674..3778
                     /locus_tag="O0394_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499609.1"
                     /translation="MLPFFILDILHIVYNIDNNNFSGVKFNDWTNKFI"
     gene            3753..4067
                     /locus_tag="O0394_001530"
     CDS             3753..4067
                     /locus_tag="O0394_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499610.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFMHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(4360..5163)
                     /locus_tag="O0394_001531"
     CDS             complement(4360..5163)
                     /locus_tag="O0394_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0499611.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYANEVTLDHNENGIPHALKKLL"
     gene            complement(5221..6135)
                     /locus_tag="O0394_001532"
     CDS             complement(5221..6135)
                     /locus_tag="O0394_001532"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCY0499612.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     SLNSQVVSCHSELVDINISGVKERIVYQ"
     gene            complement(6137..7198)
                     /locus_tag="O0394_001533"
     CDS             complement(6137..7198)
                     /locus_tag="O0394_001533"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCY0499613.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            complement(7204..8484)
                     /locus_tag="O0394_001534"
     CDS             complement(7204..8484)
                     /locus_tag="O0394_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HCY0499614.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEL
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
     gene            8675..10057
                     /locus_tag="O0394_001535"
     CDS             8675..10057
                     /locus_tag="O0394_001535"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCY0499615.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
     gene            complement(10111..10686)
                     /locus_tag="O0394_001536"
     CDS             complement(10111..10686)
                     /locus_tag="O0394_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499616.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            11017..11871
                     /locus_tag="O0394_001537"
     CDS             11017..11871
                     /locus_tag="O0394_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499617.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEISNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
     gene            complement(12014..12547)
                     /locus_tag="O0394_001538"
     CDS             complement(12014..12547)
                     /locus_tag="O0394_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCY0499618.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            12686..12874
                     /locus_tag="O0394_001539"
     CDS             12686..12874
                     /locus_tag="O0394_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HCY0499619.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            complement(12987..13589)
                     /locus_tag="O0394_001540"
     CDS             complement(12987..13589)
                     /locus_tag="O0394_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0499620.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVFLDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVIIDKLFADNRFDAWKKANEKGWI"
     gene            complement(13586..>14296)
                     /locus_tag="O0394_001541"
     CDS             complement(13586..>14296)
                     /locus_tag="O0394_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0499621.1"
                     /translation="NNYFVAMMVYYVVISLIMLDNFKKMKNREYQKEIAEKNRHINTL
                     IAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDADVEKVKAELLAINKLSRESLN
                     KVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTKQSMLVMITREA
                     INNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQRVQHLNGTLAV
                     DSTNGTKIIIEISTGGIA"
BASE COUNT         4607 a         2509 c         2021 g         5159 t
ORIGIN      
        1 ctaccgatga aactcaatcc attaatcact cttcacaaaa atttcataaa aaagcgagct
       61 ccccaatata ataggtaagc ccgctttatt ttcaaatcgt cgtatttgaa tgataagaat
      121 tacttagttt cacgatgtaa cgtatatttg tttaatcttg ggcaatattt tttcatttca
      181 atacgctcag gattattacg tttattttta gtagtgatat agttacgatc gccacattct
      241 gtgcatgcta atgttacgtt tacgcgcacg ttaaacaccc tctcttgtta ggaatcttta
      301 cgatttatta tgatatcata aactgctcaa ctacgcaata tagaaactac atatcaaatt
      361 tatttttcaa agttttcgta tgtttcatca ttttcagttt caagatcaat agaatttata
      421 tcaataccta atgcttttgc aacaccttta ccatattctg ggtcagcttt gtaacaatga
      481 cgaatatgac gtcgtttaac atcatccgtt acgccttcca ttgcatttgc tgtatttgta
      541 aaaattcttt ctttagcgtc ctctgattgt aatctaaaca atttacctgg ttgttcaaaa
      601 taattatcat catcttgacg ttgattatat tcatagccgt atccatcagt tgggaatggt
      661 ggctttttat attcaggttg agaatcaaat ttaccatggt tatttggata ataatgtgtt
      721 cctccacctt ggttattgtc aactacgcgc atttgaccat ctctactaaa aggacaaata
      781 ttttcaatac caacaccttt aggttggttt acaggaatct gccaatgatt aactcctaat
      841 cgatatcttt gcgcatcgcc atatgagaat aaacgccctt gcagcatttt gtctggagaa
      901 aaatctaatc ctggaataat attagttggt gcaaacgcag cttgttcaac atccataaag
      961 taattatctg gatttctatt taattcaaac tctccaactt caattagagg atactcatcg
     1021 tgataccata cttttgttaa atcaaatgga ttatctttat ggtttttagc ttgttcctca
     1081 gtcattactt gaatatacat tgtccatttt ggataatcac ctttttcaat ggcttcgaat
     1141 aaatcgcgtt gagatgaatc acgatctgta gctataattt cagcagcttc ttcatcagtt
     1201 aagttttcaa taccttgttg cgttctaaaa tggaatttaa cccaaacacg ttcaccagaa
     1261 tcattataca tagagtatgt gtgagaaccg aacccatgca tatgacgtaa atctttagga
     1321 atccctctat ctgacattaa gatcgttact tggtgcaatg cttctggaag acccgtccag
     1381 aaatcccagt tattttgtgc atctctcata tttgttctag gatctcgttt caccgcacga
     1441 tttaaactaa caaataactt tggatctcta aagaagaata ctggtgtgtt attccctact
     1501 aaatcccagt tcccttcttc agtgtagaac tttaacgcaa atcctcgaat gtcacgctcc
     1561 gcatcagcag caccacgttc tcctgctaca gtagagaaac gggcaaacat ttcggtttgc
     1621 ttacctattt cagagaatat tttagcattc gtatattttg ttatatcttt agttacagta
     1681 aatgtcccaa atgcaccaga acctttggca tgcattcgac gttctggtat tacttctcta
     1741 tcaaattgag acatttgctc taaaaagtaa atatcttgca ttaaaagagg tcccctaggc
     1801 cctgctgtca tactattttc tcggtctgat actggatgcc caaaaacacc agttaacttt
     1861 ttgtcttgtt gtgacatagt catccctcca caatttataa taattataat ttaatacata
     1921 ttataattta accccatcaa aattacaatt aaacatgctc aagatgatta aaaaatcaga
     1981 aaattttaat tttttcgctt tacagtacta aatagttatt gaatagtaca cttaaaaagc
     2041 ttacaataat gggtatattt aaatagatgg gagactaaat atggaagata ataaaatgaa
     2101 ccgtagtctt aactcaagac acatttccat gattgctata ggtggtgcaa ttgggactgg
     2161 tctatttgta gctactggta atatcatttc tcaagctggt cctggaggcg ctatactcgc
     2221 ttatcttgtt attggtgtca tgctatattt cttaatgtca tcaattggag agttggcaac
     2281 attttatcca gtatcaggtt cattcagctc ttattcaaca cgctttattg actcatctct
     2341 tggctttacc atgggatggt tgtattgggc attgtggtta ttagttacaa gtgttgatgt
     2401 catagtagcg tcaaatgtgc tttatttctg ggacacattt aaatttttcc accccattac
     2461 ttggagctta atctttatta caattttact attattaaac attttttctg taaaatcatt
     2521 tggagaaact gagttttggt tatcattgat taaagtgtta acaattatcg tattcgttat
     2581 ttttggcttt ttaatgattt tcggtatctt aggtggtcat acatatggat ttgaaaacta
     2641 tacaaaaggc caagcaccgt ttgttggtgg tatctctggt ttcttaggcg tattattagt
     2701 cgccggattt tcggttggtg gtacagaagt agtagcagta actgctggtg aatcagatga
     2761 ccctaaaaag tctatgccta aggcaattaa acaagtattt tggcgtattc ttttattcta
     2821 tgtcttatca attgcagtaa ttggtgcaat tattccgtac acagatccat cattattaag
     2881 agcaagtagt tcaataagtc aaagcccatt tacaattgta ttcgatagag taggcatagc
     2941 ctttgcagca tcagtaatca acgcggttat tttaacttca ttattatccg ctgcaaattc
     3001 aggtgtttat acaacaggca gaatgttgta ttccttaagt tcagacaaaa aagcacccca
     3061 atttttaagt aaattaaaca agacaactaa gttaccttta agagcattat taactactta
     3121 tgcagtcgtt gttattgtta ttatttatgc aaactttaat tcaaatgccg tttttaattt
     3181 acttgaaatt attggttcaa tgattatagt tgtttgggga tcaagcattt ggtcacaaat
     3241 acgattgcga caagctatta aaaaacaagg tcaagaccct aataaggtcc taccatataa
     3301 agcacctttt tatccattag gaccaatcat tgtcatcact acactattat tcttgctatt
     3361 tggtggctca gttgaatata ttttaaaaga tcaatggtta aatgctttta aaaacttttt
     3421 acctttaatc attctagcgt tgatttactt tattcataaa atcattcaca aaacaaaatt
     3481 tgtaaagcta gaaacaatta atttaaaacc acacgattat gacaatcaaa aataaataaa
     3541 ttctcatacg gcagatgctt tagattatct atagcatttg ccgcttttta atggtgtaaa
     3601 gcagatatac tttaatatct ttttctagta taaagtgtat taactagtat tctgtttata
     3661 ttttattatt accatgttac cattctttat tttagatatt ttgcatatcg tgtataatat
     3721 agataacaat aacttttcag gggtgaaatt caatgattgg acaaacaaat ttatttgatg
     3781 atgtactaaa gcaagatgaa agatttgtta ttgtagttca agcattagaa gaaaagaacg
     3841 gacaattatt aaagagaact ttaagggaat atcccggttt aaaccataaa caaatgaatg
     3901 atttatttat gcacttaaag gaattatttt ccgaagaatc atttgctgaa aaccaatcag
     3961 cgttcagtat tacagtttat acaaatttag attatactgc tgaccaaata tatgctcatg
     4021 taaaacgttt cagaggtaag catgactgga cacaaacagc taaataaaat caagatgctt
     4081 gcagtgattt taacaattaa tgttagattg tcactgcatt ttttaatgca ttcgttatta
     4141 ttcagaatga tcaaatttat ttatctacct tccgtgacta gttaatactt gatttattca
     4201 aaaaatatac atttactcta ttcgtttgat atcattacta atctaaaata tgtatttcaa
     4261 atcctccata gtataactga agtcattttc acaaaaataa aaagccttaa ccacaagata
     4321 tcttattcaa atatcatgtg ttaaggctta ttttaaaatt tataacaatt ttttgagcgc
     4381 atgtggaata ccattttcat tatgatccaa cgtaacttca tttgcatatt gcttaagttc
     4441 atctgatgca ttccccatag caatagctag acctgcgact tcaaacattg atttatcatt
     4501 catactatca ccaaaagcaa tgacttgatc caccgaatat cccaatttgt gacataacgc
     4561 tttaattgca ttaccttttg aaacgtcttt ggccataaat tctaagaaga atggcttact
     4621 tgtcgtagca tctacattat cattgaacac accattcaaa tcaattctag cttctgtaat
     4681 attcgctaca taatctacac ccatgacctt gggtacatcg ccttgaatat acgctttgat
     4741 atcatcaaca cgtttcatcg gtaaacctgt taattctgct tcaatattca tatactcatg
     4801 ttcgctgtcg taaataattt gaccatcatg atatgtaaga acgaaaaagc ctctatctcg
     4861 acaataatct acaatttcat cgaaatcttg cttaccaatc gatttactta cttctacttc
     4921 ttcattagtc atgttaatcg ttttactacc gttataacta ataatatagc tatgatgttc
     4981 aggtaatttt aaatctctag cagttggaat cataccttca gtaggtctac cagatgctag
     5041 aacaacataa taaccttcat cttgaattgc tgttaaataa tttgcagttt cttctgatat
     5101 cacattatct gaatttagca atgtgtcatc catatctaaa acgacaactt tataatttgt
     5161 catatcgcat taaatctcct ctctattctg catttaatac tattttacaa tataaagcgt
     5221 ctattggtat acaattcgtt ctttaacacc actgatatta atatcgacca attcagaatg
     5281 gcatgatact acctgactat ttaaagagcg aaccaattcc ccactatttt ctttacgact
     5341 aaatattaaa atagttggtc cagcaccact aattacagtt gcataggcat tatgttgact
     5401 agcaattgtt ttcacttcat caaattcagc aattaaatgc tgacgatacg gttcatgaaa
     5461 gccatcttgt tgcatgagtt tacctgctaa ttcataattg tgctgtgcta atgcacaaat
     5521 cattgtatta ctaattgcac tacttttaac cgcttcacta tgtgttaatt tttgtggtaa
     5581 agcacgtctt gatgcttctg tttttagttc ataagttggt atcgttacaa tcacatccac
     5641 gtctggtatg tcgatatgtg caactgacgt ttctttcgag acatcattat aatatccagc
     5701 gattaaacca ccataaatgg tcggcgcaac attatcagga tgtccttcga tttcagtcgc
     5761 taattgtaat acctcatgtt tagacagttg gatatcacca aaataatttg cgatatatat
     5821 agctcctact aaagcagaag ctgacgaacc taaccctctt gccaatggaa tatcacttct
     5881 catttcgata cataaattag gcaagtcaac actatattta gaagcaactt gttgtgctac
     5941 atgataaata aagtttgttt cgtcagtagg caattgctta gatgcatcat catggaaaat
     6001 atattcccat tttgtccctg atgtttcctt tacagacaga tgcaaaaatt tatctaaagc
     6061 catacctata gaatcaaagc caactccaag gttggctgtt gatgcaggaa ttgttaactc
     6121 caaaacattc gacatgttat aaagctcctt taatataatc gataatgcta tctttatcat
     6181 ttggcaatgg ctttatcgga ttatctagta gtgaaatagc agtatcagga tcttttaatc
     6241 cattaccagt taaaatagca actacttttt taccttgagg taatttacct tgtctatgca
     6301 atttaattaa acctgcaata gaagcattac tcgctggttc actaaagaca ccttctttag
     6361 ttgtcattaa ctgatatgct tctagaattt catcatcagt aacactatct attaatccat
     6421 ttgattcttt aagagcatta gtcgccttat cccaactagc aggattacca attcgaatag
     6481 cagttgcaat cgtttcagga tttttaatga ctttattttg aacaattggt gatgcgcctt
     6541 cagcttggaa gccaaacatt ttcggcaatt gtgatccttt agcttcatga tattctttaa
     6601 agcctttcca atatgcagta atattacctg cattacctac aggaatcgct aaaatatcag
     6661 gtgcttcacc gtctaattgt tgtacaattt caaatgagcc tgtcttttgt ccttcgattc
     6721 taaatggatt gacagagttt acaagctcga tttcgccact ttttgcaatt tcttttacaa
     6781 tttctaaagc ttcatcaaag tttccttcaa tagaaacgat ttctgcacca tacattactg
     6841 cttgcgacaa tttacctaat gcaattttac cttctggtat tacgacgata gcttttaaac
     6901 ctgctctcgc tgcatatgct gctgcagacg ctgatgtatt tccagtcgaa gcgcatatta
     6961 caattttctt accttgctct tttgcttttg tcacagccat taccataccg cgatctttaa
     7021 atgaacctgt cggattggca ccttcatact tcacatataa atctatgcct agtattttag
     7081 acatattttc acaatgaatg agtggtgtat ttccctcgtt caatgttaat tttggtgtat
     7141 tttcatttac tggtaaatgt gctttaaact cttctactaa tccttgccat cttctcatta
     7201 taattaaact ccttctactg ggtatacttt gtcaacttcg tatccatgct tagtgattaa
     7261 ttcttccggt gatgattcca atccaactat aacagcggca tatgattgat tatcgtaatt
     7321 tgcaactctt aatgatcggt gaaatggtaa tattgccttt aactcatttt caaaattttc
     7381 aattgaacct ttgacatgat tcactactac aaagaaattg gatttttctt taatattaat
     7441 ttctgcatct gaatccatca tttcccgtgt tttatctgtc tttaattcaa aatgtggtgg
     7501 caatgtgtgt aaatctgatt caaagaataa tgctacattc aataaatcac tgacaacggc
     7561 actacctgtt gctaaactgc ctgctccttt tccataaaac atcgtgtcac caacggcatc
     7621 accaataaca taaatcgcgt tatattcatc ctctacagct gctaattgat gctttttatc
     7681 aattaacgtt ggttctaccg atgcattaac tttgccattt tcatatattc ccttaccaat
     7741 caatttaatt ttatacccca gttgatcggc tacattaata tcagttaaag ttacgccact
     7801 aattcctctt cgtttaacgt cgtttaattt aatgacttgg ttaaatgata aatatgatgt
     7861 aatgacaact ttacgcgctg catctacccc ttctacatca tcagttggat ccgcttcagc
     7921 aaaaccaagt cttttcgctt catcaagtgc ttcctcaaat gtcgtttgct ctttagtcat
     7981 tttagataaa ataaaattag aggtaccatt taaaattccc ataaattttg aaatattatt
     8041 cgcattcaaa ccattattta tggcatttac gatcggaata ccacctgcta cactcgcttc
     8101 aaactttaaa gctacaccat tttcttctgc taaatcttct aaaagtttaa gatgtactgc
     8161 taataaatct ttatttgcgg taataacatg ttttttattt ttaagtgctg ttcttaacca
     8221 atctacagtt ggttcaattc ctcccatgac ttcaacgata atatctaatg aatcatcatt
     8281 taaaatttca ttaacatctt cagttaaatg atattggcta atatttagcg gtctcttttt
     8341 agatttatct cgaacaagaa tatgctttat gacaatatct ttatttaatg tatcttgaat
     8401 ttgctgtcgg ttctcttcga tgattttaac aacaccagat ccgacagtac ctaatcctaa
     8461 taatgctata tttaattttt tcattttatg tacctccgtt tgtgcttttg agaaaaacaa
     8521 atgtattatt gaaaaacact agatgatagt atagtataga attattcttt atttttgtaa
     8581 agttcgtaat attcaaaata taaatacata atatcattct tacttacaaa actgaataca
     8641 attttctgat tttttagaaa ggttggcatg cactatgaaa gtttccaaat tcggtggtag
     8701 ctccgtctcc aatgctactc aaattaaaaa ggttttaaat attgttaatt ctgatccaga
     8761 gcgaaagatt gttatcgttt ctgctccagg taaaagacat gataatgata ttaaaacaac
     8821 tgatttgtta atcagattat atgaaaaggt cattaatcat cttgattatc atgataaaaa
     8881 aagagaaatt attcagcgtt atgatgatat tgtaaaagaa ttgcaaatgg atgaaagtat
     8941 tttacggacg atagatgtga ctttggaaca ttatattaat caattaaaaa atgaaccaaa
     9001 gagactatta gatgcattac tttcttgtgg tgaagatttt aatgcgcaat tgatagcttt
     9061 atataataat agtcaaggta taccaacaaa atatatatca ccgaaggaag cgggtatttt
     9121 tgttactgac gtcccacaag aagcacaaat attagatagt gcttactctg aaattaaaaa
     9181 attaagagat tatgatgaaa aactaattat cccaggattt tttggcgtat ctcatgaagg
     9241 atatatagtt acatttccac gtggcggatc agatataact ggcgccatca tatcaagtgg
     9301 tgttagagcc acaatttatg aaaatttcac cgatgtgtct ggtatttata aagctaatcc
     9361 aaatatcatc aaagatcctg aattaatcga agaaatcact tatcgagaaa tgcgtgaatt
     9421 atcttatgct ggttttagtg ttttccatga tgaagcctta caaccacttt ataaagatcg
     9481 catccctgtg gtgattaaaa atacgaaccg ccctcaagat aagggtacat ttatagtgca
     9541 tgatagagaa ataaatgcga aaaatgtgat tagcggtata agttgtgata aagattttac
     9601 cgttattaat atcaaaaagt atttaatgaa tagacaagtc ggctttacta gaaagatatt
     9661 aggtgtctta gaggataata atatttcatt tgatcatatg ccttcaggta ttgatactat
     9721 cagtatcgtt atgcgttcaa aacaaattca aaataaagaa acaaaagtat taaatgaaat
     9781 tcgtcaaaaa tgtgatgttg atgagctaag tattgagcat gatttagcaa tattaatgat
     9841 tgtcggcgaa ggtatgaata aagtcatcgg tactgcaaat aaaattacac atgccttagc
     9901 tgaatcaaaa attaacttaa aaatgattaa ccaaggtgct tcggaaatct cgatgatgtt
     9961 tggaattcat gtagatgatg ctgaaaaagc tgtattatct acatatgagt tttgctatca
    10021 cggggttgca ttaaaaaact tgtgtgataa aaactaatat aaaagcaatt cgtaacacat
    10081 gtattcaact gcgttatgaa ttgctttttc ttaattattt attgttctct ggattgcatc
    10141 cttaatacgt tgctcaccta ctacaaattt taaaataaaa aattgatagt agcttaatgc
    10201 atctatgact cgtttataat tggaataatg atgatactga tggtgttgca ttagatcata
    10261 cattagaacg atctccggtt taatataatc cacatcccaa tttaaactat ggaagtatat
    10321 gttactctta ggtatacgta tattcggatc caatctaaaa atccattcat cgtttactac
    10381 atcataaacg aaaatactca ttattgttgt atcatcatat tgcacataaa cttttgcaat
    10441 ttttgaaagt gataaatctt ctttagctat tgcttgatct tgcttatcta tgtatagtaa
    10501 cgcatatgat tgcggaataa ctttcacaat atcacttaaa cgcttacgtt ccacgcatat
    10561 ttcaacaata gaaggtaatt ttaattcatc atttaagaaa agctgcatgg caacttcacc
    10621 gtgaaattta aataattgcg atgatgtttt cattaaattg tccactaatt gttctttagc
    10681 attcatttca ttcactctca aatacacctc ctaagcaagc gctttcacaa atatcattat
    10741 acattttaaa attaatgaat tcaatcttca aaatactatg agtgacctaa ttctgtcatt
    10801 taaatgaccg ttataacata aattcgacat aaacttgaat tcacgaatct ttcacgtcat
    10861 attcattgaa catcaatatc tgtcagttta taatttgatt ataacaatga tgaactaaaa
    10921 tcaactaata aaaatgtggg aaattgaata acaattaatc tttattaatt attattgtat
    10981 acaaagttaa agaagtcaaa aaggattggt gaaaaaatgt ctttttatgt tgtacttatc
    11041 attatcattg tagcattaat tggtatatta gttttaaatc aaaggtatag taatagtaaa
    11101 atagataccg aagtttatgc aagaaagcaa cttattaaaa agaacaaagc attaagtgca
    11161 gaaaatgctg aattaagaag tcaaatgctg agttctaata atgatgttgg ccaccatgca
    11221 tacaaaaatg ctaaaagaga attacgcaag attttagata gctatctcga aaatggtaaa
    11281 ttaaaatatt acgatattat cgttacaagc aatttagcta ctaaacatcc gtttttcgag
    11341 tatgcacgtt catttgattt tatcattgtt tcagatatcg gtttaataaa cgttgatgta
    11401 aaaagctggg gtgaaaaaac attttatcac tttgatgtac ctgatgagca tgatacagaa
    11461 ataagcaata gtaatattga aaaagttgtc ggtcattaca ttagccagca atatcatgat
    11521 cagtttaatt catcaagaaa gtctatctat acttttacag aaacagttca accaaatcgt
    11581 gttatttatg atttttatga ttatgatccg tatcaattag cagcaaataa tgcgaaagca
    11641 ttaaaggatc atattgaaca aaactttaac tttaaagtcc aaagtaccgg tgttatttac
    11701 tttagtgatg gtacagttaa tatcattcaa ggttctgaag aaagagataa atatgttgat
    11761 acagtttcaa caaaatcttc gttacgacgt attattagcg aagccataga gctatctaaa
    11821 caccctttaa ataaagaaca ggttgatcaa atcacagcta tattcaagta aaatcaaagg
    11881 cataataatc cattacgctc gatacgcact atggttatta tgcctttaaa ttatttctat
    11941 atataaattt atggtaataa taaatcgatg cttcaagtat attttacttt aatatatata
    12001 atcacctatt taattattta ctccaaatat ttaatttctg ttgtttagct ttattttgtg
    12061 cttctataaa tacatttcta tatttgccat ttggtgaaaa atacttctct ctagcaagtc
    12121 ccttttccac taattcctta ttgtacatac gatctttact tatccataca tacgccaatg
    12181 ttctaccata gcgatcttgt ttttctttat catattctaa ataaacatct tgatttgtta
    12241 atgtcttctt actataattt gatgcttctt tgccaaatgg ttgtacaggc gtattcggtt
    12301 tcaccgtttc tggcgtatca acccctataa gcctaacttt aatttcttta ccattttgat
    12361 ttgcaataaa tgtatcacca tccacaactc tttgaacatg tactttatct ttaccattta
    12421 aatcttgtac agtttcatga ttcgtcccct ttttgaaagg acccgtatga ttcataaatt
    12481 gaaatgctaa tacacctaca ataatgatgg ctaccacaat catagcaagc gatttatttg
    12541 acttcatatt gccacctact tctctttttt cattccgatt aatcatataa aagttaagaa
    12601 atatcgtcaa tagccatatc tacatatttc aatattttgg aaagctatag catgatataa
    12661 tgttattgtt aaggagtgaa aataaatgaa catttcaaat gaaaatacag tcacattaat
    12721 tgcagtaatt atcgcaatta tcattggtat tttcctacaa atatttttca aattaccatt
    12781 aatagtgacg gcagtactat ctattttatt gggtatcttt gtaggcttta tcgtttactt
    12841 gattgtttcg ttttataata agcgaaaaaa ttagttaaca aatctaaatt gcgagtacca
    12901 aaataaaagt attgagtaag attttgatta ttgactcact ttccaagtct cttaaatcaa
    12961 aacactcaat acttttttgt ttgtatttag atccagcctt tttcatttgc ctttttccaa
    13021 gcatcaaaac gattatctgc aaataattta tcaattataa cagatgtata atttctaact
    13081 gttccatctg tcaaaaataa tttttcactt atttctttac tacttaaacc attgccaatt
    13141 tcccttaata caatttgttc tttgggcgtt aatgggtttt tatctacaaa aaatgaagtc
    13201 atcaatgtgg cgctatattc tttctctccg ttatttactt tattaatggt ttccaccaat
    13261 tcttctatag aacgttcttt taaaacatat gcatccacat cattcacaac tgctttttca
    13321 aagtatcccg gtcttttaaa agttgttaca ataatcactt taatattcaa atgctttttt
    13381 ctaatttccg ctaaaacttc aagtccagtc atgcctggca tttctatatc taaaaaaaca
    13441 acgttaggat tatattcttc aataagtttc attgcatcga gaccattatc agtatctgct
    13501 aaaatttcaa aatcaccatg tagtttaatt aattgaacca ttgcctgtcg taacatattt
    13561 tgatcttctg caataattaa agatgtcatg ctattcctcc tgttgagatt tcaatgatta
    13621 ttttagttcc atttgttgag tcgactgcta aagttccatt taaatgttgt acacgctgtg
    13681 aaatactttt cacctcacaa tcactatcga tacctttgcc atcatcttca atcataagca
    13741 gtaatttatg attgtttaca gtttttaatt taccatgaac ttttgaagca tttgcatgtt
    13801 taataacatt atttatcgct tcacgcgtaa tcataactaa catagattgt ttagtaggac
    13861 ttaatacttg cgctaattct ttattttcaa atgtaaaatc aatatcagca tcttttaaaa
    13921 ctttacgtat actatcaatc tcttcaataa atgatggtaa ttttacatca tcaataattt
    13981 ctcgaacttt gttcaatgat tcacgagata atttattaat tgctaataat tcagctttta
    14041 ctttttctac atcagcatct attagtttat aagctaattc tgattttaat gataaacttg
    14101 caaacacatg ccctaacgta tcatgtaagt cttgaccaat tctatgtcgc tcttgttcag
    14161 caattaatgt attaatatgt ctattttttt ctgctatttc tttttgatat tcacggtttt
    14221 tcattttttt aaaattatct agcattatta acgatataac gacataataa accatcattg
    14281 caacaaaata gttgtt
//
LOCUS       DAKTLH010000063        14238 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.063, whole genome shotgun sequence.
ACCESSION   DAKTLH010000063 DAKTLH010000000
VERSION     DAKTLH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14238
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.063"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            484..1350
                     /locus_tag="O0394_001542"
     CDS             484..1350
                     /locus_tag="O0394_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCY0499622.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
     gene            1370..1870
                     /gene="queF"
                     /locus_tag="O0394_001543"
     CDS             1370..1870
                     /gene="queF"
                     /locus_tag="O0394_001543"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCY0499623.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
     gene            2211..3716
                     /locus_tag="O0394_001544"
     CDS             2211..3716
                     /locus_tag="O0394_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCY0499624.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAISPLIIKAMKGIR"
     gene            complement(3794..3895)
                     /locus_tag="O0394_001545"
     CDS             complement(3794..3895)
                     /locus_tag="O0394_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499625.1"
                     /translation="MVINEIDFYRVGRENIVIAVYNEHIKFLFILIS"
     gene            3986..4903
                     /locus_tag="O0394_001546"
     CDS             3986..4903
                     /locus_tag="O0394_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCY0499626.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            complement(5527..6069)
                     /locus_tag="O0394_001547"
     CDS             complement(5527..6069)
                     /locus_tag="O0394_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HCY0499627.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLRESGFFRHLKVMPYAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
     gene            complement(6420..7478)
                     /gene="hisC"
                     /locus_tag="O0394_001548"
     CDS             complement(6420..7478)
                     /gene="hisC"
                     /locus_tag="O0394_001548"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCY0499628.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
     gene            complement(7718..9232)
                     /locus_tag="O0394_001549"
     CDS             complement(7718..9232)
                     /locus_tag="O0394_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCY0499629.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            complement(9225..10202)
                     /locus_tag="O0394_001550"
     CDS             complement(9225..10202)
                     /locus_tag="O0394_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0499630.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQLLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            complement(10424..12205)
                     /gene="recQ"
                     /locus_tag="O0394_001551"
     CDS             complement(10424..12205)
                     /gene="recQ"
                     /locus_tag="O0394_001551"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCY0499631.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            complement(12217..14100)
                     /locus_tag="O0394_001552"
     CDS             complement(12217..14100)
                     /locus_tag="O0394_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCY0499632.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LIEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREENEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
BASE COUNT         4489 a         2523 c         2042 g         5184 t
ORIGIN      
        1 ttcttcataa ataaagcatt tgcatacaaa acacaagata ttgtgtcttt ttcgtttcac
       61 caacactata ttatgtgttt cattatacat aagacacact atgatttaaa gtgccatttt
      121 ctaatcgaat gttcgtaaaa cgttccaact aggcaccaat actaaattag aaaaacttga
      181 acaaatttaa aaaaattttt tagtagatat cacttgattt ttttaaatct gtgtcaatct
      241 caattgtatc aacagaaatc aagaaaaagc ttattagctt gatactgaat tgatatttgt
      301 tttatgtaat aacacaaaca ttcataaaag tgtaatgccc tattattccg acaaataaac
      361 acagaacgtt tgttcgctac agtctattat agtcttttta tagtaaattt caactattta
      421 tgcacaacat gttgttttat gctaatataa atacgttaat tttatataaa agaggcgata
      481 aaattgaatc ctaaagtaaa aggtatcatt gccattttaa tttcggctat tggtttcagt
      541 tttatgtcag tgtttttcag acttgcaggt gaccttcctg tttttcaaaa atcattagca
      601 cgaaacttag tagcaatgtt tattccatta ttttttatat ataaatacca tcagccaatg
      661 tttggcagac tttcaagtca acccttatta ataacacgat caacacttgg acttattggt
      721 gtattgttaa atatttatgc cattgatcat atggtactta gtgatgccga ttcattaatg
      781 aaactcaatc ctttctggac gattttatta agcattgttt tcttacatga aaaggtaagg
      841 aagtatcaaa ttacagcaat gataattgct attttaggta tgcttttaat agtgaaacct
      901 gaattttcat catctatgat tccatcatta gctggcttat tctcaggtat ttttgcagca
      961 tcagcatata catgtgttcg agcacttagt acaagagaag caccatatac tatagtattt
     1021 tacttctcct tattttctgt catcgtttta atacctttca ctgcatatac ttatgaacca
     1081 atgagtcaga tgcaaatact ttatctatta ggggctggct tagctgccgc tgtgggtcaa
     1141 attggtgtta ctttagcata tagttttgca gcagcaaaag atatttctat ttttacgtat
     1201 gcatctataa ttttcacagc aatacttgga tttattctat tcggagaatc tccagatttc
     1261 tatgcaacat taggttacgt cgttattatc ggtgcaagtt attacatgtt tgaaaaagca
     1321 cgccgagacg ccaaaataat aaaaaaataa atttaaagga gcgctttata tggcacatgg
     1381 ccgtcaacaa gatgaattac aagatataac tttattagga aatcaagaca atacttacaa
     1441 ttttgactat cgtcctgatg tattagaatc gtttgataat aagcatcaag gtcgtgacta
     1501 tttcgtaaaa ttcaactgtc cagaatttac atcattatgt ccaattactg ggcaaccaga
     1561 ttttgcaact atctatattt catatattcc aaatgttaaa atggttgaat caaaatcttt
     1621 gaaattatac ttatttagtt tcagaaatca cggtgatttt cacgaagatt gtatgaatat
     1681 tattatgaat gatttgatag agcttatgga cccacattat attgaagtct ggggcaagtt
     1741 cacaccacgt ggtggaattt ctattgatcc ttatacaaac tatggacgtc caaattctaa
     1801 atatgaaaaa atggctgagc atcgtttgat gaatcatgat ttatatcccg aaaaaataga
     1861 taatcgttaa atgtatcatt taataaacac accaataagt tgattttcct aacttattgg
     1921 tgtgtttttc atttagcata cataataggt tacattaaaa taacatttta taccaaagta
     1981 caccaaaaga atattagtac acgaattaaa caacattttt atagaaacct attgcacttt
     2041 aacgtcaata agtatatttt tatattatct ctaattaatt gtgcgcgctt aataacagaa
     2101 tattctcaat atttttattt ttttgtgatt tgttggaata tttagttgat aaggcacaat
     2161 caaatttact taaactattg tattagggga agaaaggatg ggatgtatac atgacacaac
     2221 aaaactccca tggaaatcaa attcaagaca tacctcaaac aggatttttc gggcatcctc
     2281 gaggactagg cgtactcttc tttgtagagt tctgggaaag gtttagttat tatggcatgc
     2341 gtgccctact cattttctac atgtactttg ccgtaacaga taatggcctt ggaattgata
     2401 aaacaacagc tatgtcaatt atgtcagttt atggttcatt aatctatatg acatcaatac
     2461 caggcggatg gattgctgac agaattacag gcactagagg cgctacttta ttaggtgcag
     2521 tctttattat tatcggacat atttgtttaa gcttaccatt tgcattaatc ggcttattca
     2581 catcaatgtt cttcatcatt attggttcag gtttaatgaa gccaaacatt tcaaatatcg
     2641 ttggccgttt atatcctgaa aatgatagac gtatggatgc aggttttgtt attttctata
     2701 tgtcagttaa tatgggtgca ttattatcac ctattatttt gcaacacttt gttaatgtta
     2761 aaaacttcca cggcggattc ttgattgcag cagttggtat ggcattaggt ttagtatggt
     2821 atgtactttt caaccgcaaa aacttaggta gcgttggtat gaaacctact aacccattga
     2881 caccagctga aaagaaaaag tatggtctta ttatcggaag tgttgtttta gcaattgtat
     2941 taattatcgt tattggtgca ttaactaact cattatcatt taacttagtt agtaatactg
     3001 ttttagtatt aggtattgca ttaccaatca tttacttcac tttaattatt agaagtaaag
     3061 atgtcactga tactgaacgt tctcgtgtta aagcatttat tccattattt attcttggaa
     3121 tggtgttctg ggctattcaa gaacaagggt ctaatgtatt aaacatatat ggaattgaac
     3181 attcagatat gaaattaaac ttatttggtt ggaaaacaaa ctttggtgaa gcgatcttcc
     3241 aatcaattaa cccattattt attttattac tagctccaat tatttcactt ttatggcaaa
     3301 agcttggaac taaacaacct agcctgccag taaaatttgc aattggtacg ttcttagcag
     3361 gtgcgtcata catactaatt ggtattgtag gttatgcatc aggttcatca aacttttcag
     3421 ttaactgggt tattttatcg tacattattt gtgttatcgg tgagctatgc ttatcaccaa
     3481 cgggtaatag tgctgctgtt aagttagcac ctaaagcatt taatgcccaa atgatgagta
     3541 tttggtactt aactaacgct tctgcacaag caatcaatgg tactttagtt aagttaatag
     3601 aaccactagg tcaaacaaat tactttattt tcctaggtgt tgttgcaatt attgttacaa
     3661 caattgtatt agcaatctca cctttaatca tcaaagcgat gaaaggtata cgttaatatt
     3721 gttggcctaa ttcaaaaaac agtaagtcat ttaaatggct tactgttttt ttataggttt
     3781 ctattaatta aattcaagat atcagtataa ataaaaactt aatatgctca ttatagacag
     3841 ctataactat attttctcgt cccactctat agaaatcaat ttcatttatt accattttct
     3901 ctatttaaaa acatattttt ggtattaaca attaaaagtg ggtatatata tttaatgaac
     3961 caatttttag aaagtaggct aaactatgga aaataaatat acacatggtg tcctctttta
     4021 ccatgaacac agtggattaa aaaatattaa tcaaggtatt ggggaagtta caacagcatt
     4081 aagttcaatt tgtaagcatc tttctattca attgagtgaa aacgaaggtg acattattaa
     4141 atattgccaa gaaatcaaaa ctaaaaacta tgcaaaagat gtagatatat tatttattct
     4201 tggcggtgat ggcacggtta acgaacttat aaacggtgtt atgacacatg accttcaact
     4261 ccctattggc attttaccag gcggtacttt taatgatttt acaaaaacgc taaatatagc
     4321 acctaatcat aaacaagcta gtgaacaaat gatttctgca caagttggta catacgatgt
     4381 aattaaaata aataatcaat atgcactcaa ctttgttggc ttaggtctca ttgttcaaaa
     4441 cgctgaaaac gtacaagatg gttcaaaaga tatatttggt aaattgagtt atattggatc
     4501 aactgtcaaa acactgctaa atccaacaca atttaattat caattatcaa tagatgataa
     4561 gacatattct ggcgaaacga ccatgatact aacagcaaat ggtccattca ttggtggtag
     4621 tcgtatacca ttgactgatt tgtctcctca agatggtgaa ctgaacactt tcatttttaa
     4681 tgaacaaagt ttcagtattt taaatgatat ttttaaaaag cgtgatagca tgaattggaa
     4741 tgaaattact caaggtattg agcatatacc tggtaaaaag atttcactta caacagatcc
     4801 agcaatgaaa gttgatattg atggcgaaat ttcattagaa acacctattg atattgaggt
     4861 tataccaaat gccattcagt tacttactgt caatgatttg taagcatccg gaatgatctt
     4921 aacccgatat tatattaggt ttggagtgta atcataagat tcaacgcacc ttatattcgt
     4981 ttttattagc tttaagctat taccctgttt caaaaacaga catataattt tggcgcatgc
     5041 cactaaaatg attgatgttg tattttaaat catagtggtt tatgcgtctt tttcaaattc
     5101 tataaaaaat cggatgacgt gtaatctgcc atagattaac acattcatcc gatttataat
     5161 aataagataa actaacattt attgagagcg ggacggaaat gataaagaac gactaatgat
     5221 tgattatgta gcgattcttt atcattagtc acagctaatg tgtacttaaa aatatgaatg
     5281 catgagttac actcatgcat aagaaatact aatttctaaa gaaaaagtat ttctttatgt
     5341 tggggcccac cccaacttgc attgtctgta gaaattggcg ttccaatttc tctatgttgg
     5401 ggccccgcag tcaactactg ccaatatagt gttgtagtgt ctaagacata aaagttcatc
     5461 tcagtcacaa ttactttata gcctatccat catcttttct acatgaaatt tttcaagtga
     5521 tatattttat ttctcaatat tatctaaaaa atactgttct acatctttcc agctatttac
     5581 gcgttcaaat cgatcatcat taatattatg cactgctgta aacataatcg gtgtaccagt
     5641 aaaaatttca agctgtctag gattgtcatc tattaaataa tcagctttaa cgatgttttt
     5701 tctaccacaa aaaacaaaat gctgaggatc taaaaatgga aagaactcta gtaaccattc
     5761 atatttatca ctaaatgatg ttggtacatc cattgctgct gtagcaatat atacatcata
     5821 atgttcagtt aattttttca caacttcttg tgcatacggc atcactttaa gatgtctgaa
     5881 gaagcctgat tctctcaata cttctgtaat taatccatca tgttcaggaa taacatgttt
     5941 aagtttttga ccattcaaag cttccatttt aatacctaaa tccgctctaa aattgacagc
     6001 atcaatgatt tctcctaatg tatctgccaa tacttcatcc atatcaatcg cgattgattt
     6061 acgggtcatt ttaatcttct cctcaaaata tcagtattga ttcagtatag caaaaaagca
     6121 attgatacaa aacaatatca tctattgttg ctttttacac tgaatactct cacctcatca
     6181 ttcacactgc attgagcttg agacataaat caatgttcaa tgctctacaa agttatattg
     6241 gcagtagttg actgaacgaa aatgcgcttg taacaagctt ttttcaattc tagtcaacct
     6301 tgccggcggg gccccaacaa agagaaattg gattcccaat ttctacagac aatgcaagtt
     6361 ggggtgggac gacgaaataa attttgcgaa agtatcattt ctgtcccact ccccacttac
     6421 tattcgtatt taaagtttga taaaacttct aacattttat cattttgttc tttaaaacca
     6481 attgtaattc taacaccagt tggaaatggt cgcgtaatac accctacatt taaaagtgct
     6541 tcataaagtt catttacccg ctttgttttt acaaatataa aattcgtttg acttggcaag
     6601 aaatactcac tttgaggtaa ttgataaaat ctttcgcgtt caacactatt tttatgtgtt
     6661 acctctttta aatattgttg atcttcaagt gctgcaactg ctgcgtattc agatatacgt
     6721 gtcacattaa atggtggtct aatgatgttc catttttcaa tcgcatgttc acttgctacc
     6781 acatatccta cacgtaaacc cgctaatcca tacgcctttg aaaatgtacg taataagaaa
     6841 gcattgtcat atttttgttg caaagcaagt gtatccgggt agtcctctgc tgtcacaaat
     6901 tcaaaataag cttcatctat aattactggt acatgtggag gtacttgaga taaaaacgaa
     6961 tctaagctct catgattaaa atatgtacct gtaggattat ttggattaca taaccatacc
     7021 aatgacgtat cttcattaac ttcttttaaa ataccttcta aatcgaagcc accatctttt
     7081 aaaggtactt gtatcacatt agctgattca acaatcgcat tgtgataata ttgaccgaat
     7141 gtcgcttcac ttgtaacaat agtatcccct ggcgttaata cagctctaga aatcattaat
     7201 ataacttcat ctaatcccgc accaaataaa atgcgtgatt gatctacatt taaatgttta
     7261 ctaatcgccg cttttaatgt cggtgatcct gtttcaggat aataatataa ctcatctaag
     7321 tgtgctgata tcgcttcttt aactttaggc gatggtccat acaaattttc atttgatgca
     7381 agtttatata aatctccttc aatgccatac ttttctttca atgcccttgg agataaacca
     7441 ggctgatatg ctgatagttg attaagttgt tctttcataa taaaaacaac ctccttagaa
     7501 ttaactgaaa tttcaaaaaa cttatattca ttatatcgct ctatgtttct aaaatgcaat
     7561 gctactattt tcacatcata atagcttaca ttgtgtttaa catgtcatat tttttaatca
     7621 ttattgcatg tttaaattga acataattat agatttaaat ataacaacgc cacataagat
     7681 ataatttggt gttacgcatc ttatgtggcg ttggtcgtta tttgattaac ccttttgctt
     7741 ttaaataatc tttcgcaact gtatatgggt cttcattttt aactgttact ttatagttca
     7801 tcatttgcat atcttcatca gatattttgt tttctagttt gtttaacggt ttcttaattt
     7861 ctggatgttt ctttaaaaag ctttctttaa ataatggtgc tccttgatat ggtggaaata
     7921 cgtgcttatc atcttttaac acaaccatat catattgttt taattcagcg tcagttgaat
     7981 atgcatctat taaattaata ttacctttat tgatcgcttg atatctcaac ttaggttcca
     8041 ttgtacgtat gttatctaaa tttaaattat aagccttttg aacagctttg taaccatctg
     8101 gacgatcatt aaattccaat gtaaatcctg gtttaagttg atctttaacc ttatttaaat
     8161 caccaattgt acgtatatta tgttgtttag caaaatcacg ttttacagct aaagcatacg
     8221 tattgttata cttcattggt tttaacatag tcatttgata tttcttttca agactttgct
     8281 tagcttgttc ataaactttt ttctcttctt ttgacttcaa tggttctttt gttaattcac
     8341 ctaaaactgt tccagtaaat tctaaatacc catctatatc gtcagatttt aaagcattaa
     8401 ataaaaatgc tgttttgccc ataccatctt tcacttctac agtatttttg gtctcttctt
     8461 ctattaaaat tttatacata tttgtaataa tcgatggctc ggagccaagc tttccagcta
     8521 acgtaatttt atcacctttt tgtgcaaaca taggaatagc gatagccagt ataataatca
     8581 tcactataac acctaacgtc atcaataact ttttataaga taatttagcc ataaatctta
     8641 aaattaaatc aaatataatt gccaataagg ctgctggaat tgcacctaat aatatcaacg
     8701 atgcattgtt acggtctata cctaataaaa ttaaatctcc tagtccgcct gcaccaatta
     8761 atgctgctag tgttgctgta cctataatta ataccatagc cgttcttaca ccagccatta
     8821 taacaggcat tgctatcgga agttcgactt tagttaaacg tctaaatggt ttcataccta
     8881 tacctttagc cgcttcaatg agtgatggat caacttcttt aattccagta tacgtattcc
     8941 ttaaaattgg taacaacgca tacactacaa gtgcaataat tgctggcaca cgaccgatac
     9001 caaataaagg aatcattaaa cctaataatg ccaacgatgg tatggtttga agaattgccg
     9061 caatattcat tacgatttca gatatcgttt tagtcttcgt taataaaata cctaatggta
     9121 ccgcaatagc agttgcaatc aataatgcga taaatgatat ttgaatatgt tctatcattg
     9181 tcgaaaagag ttgcccctta cgttcactca atatgtcgaa aaagttagtc atgttgagct
     9241 acctcctttt tctgggacaa atatttgaag atatctttcc tatcaataac atattgacct
     9301 acgctatctt cttgcatgac aatgacacgc tcgctctctg ataaaagttg atacaatact
     9361 tcaattggtt gattgtcata aacaattgga taagcgctca tagatgtaac ctcatcgatt
     9421 ggtttcataa tatccaagtc acggataatt gcgttctctt caacacatgg cgcatcatct
     9481 tctaaatgac tacccataaa ttgtttaaca aattcacttt gaggattatt tttaaatcct
     9541 tctggtgtgt caatttgttc aatatgccct tcattcaaaa gacaaatctt atcaccaagt
     9601 ttcatcgcct cttgaatatc atgtgtaaca aatatgattg tcttcttaat tttagtttgt
     9661 aattcaatta aatcatcttg aagtttttct cggctgattg ggtctaatgc actaaacggt
     9721 tcatccatta aaataactgg tggatcagct gctaacgcac gtataactcc tacacgttgt
     9781 cgttgccccc ctgacaattc atcaggtttt ctgtttttat atttttcagg ttctaatcca
     9841 accatttcaa gtaattcatc tactctttta tctatatctt tttctttcca ctttttcatt
     9901 tgtggcactt gtgcaatatt ttctttgatt gtcatatgtg ggaataatgc aatctgctgc
     9961 aatacgtatc caatatccca acgcatttcg tatactggat aatcacttat tggtttatct
    10021 ttaaaataaa tataaccttc acttaagtga atgagtcgat taatcatttt taatgtcgta
    10081 gtttttccac aacctgaagg tccaattagc acaaaaaatt caccctcatt aatattgaaa
    10141 ctaatgttat cgacagcaac atgtttgcca taacgcttag ttacattttt aaacttaatc
    10201 actttgccac ctcttttttt ctcatagcat aaaaccgaga ttatatgtat gtattcccta
    10261 tttaaccacg tttattacaa ttttcaaatt taaatgattt atccttgaac ttttttaata
    10321 aaataatgaa taataggtaa tctccagtta agaaatagtg ttattttacc ttgaattcaa
    10381 aaaaacaccc agtaaaacaa ggaatgctta ctaggtgtct tcactatact ttggctttat
    10441 aattttgaat cgtttctaaa aatgctggac aataatgttt taatttgtaa ctacctacgc
    10501 catcaatatt aatcatatct tgtttcgaag caggcttacg tttagcaaat tcctccaacg
    10561 tgtaatcaga aaatatactt acaggtgcta tcgttaattt gtcacttaac tttttacgaa
    10621 cttctaccaa ctgactgaat aatactcggt caaccccttc aaccgtattt ataaatactt
    10681 tttcagtcgc tttttgctta aatggtgttg tgaatacttc tacttcatta ctgagtaatt
    10741 ttttaattga agtatcacac attaatattt cgtcattttc atttaagaac cctttgaatc
    10801 ttaattcatc tattaagtga cttaattctg atgttgtgta acctttcatt aaaccatggg
    10861 ttgaaatttg gtcataacct ttatacttaa tataatctgt tgactctcct cttaacactt
    10921 gaatgataac actataactc tcttgttgtt tcatacgagc gatgcaacta ataatcatct
    10981 tagcttcttg tgtcatatta tatgatttat cttgttgaac acaattacta cattgttcac
    11041 attcttctaa tttttcattc ggttcaaaat aatggacaat tgttgcttct agacattttt
    11101 ttgtttttgt atattgaatc attttagtta acttttcgcc cattttatct ttatagtcat
    11161 catcagcttg agagactgtt ataaaatact cgtgtaaatt gatatcgcgt tcgctaaata
    11221 acaaaataca ttcacttttc aacccgtcac gacctgcacg acccgcttct tgataataag
    11281 attctaaatc tccaggcata ttataatgaa taacaaagcg tacattggat ttatcaatac
    11341 ccataccaaa agcatttgta gcaacgacta ctttaacacg atcaaataag aaatcattct
    11401 gcgcttcttc tctttcttta ttgctcaaac ctgcatgata tataacactt tcaattttct
    11461 gactttctaa ggcttcttga agctcttcaa cttgcttacg tgtagaacaa taaataatac
    11521 ctgcatcttc atcgtgtgtt ttaatataat ccaatataaa tttttgacgt tgataagtag
    11581 gatttacttt aaaaattaag tttctacgct tagtactcgt tttaatttga tcagtttgag
    11641 cgatatttaa cttttctcta atatcttgct gtacttcaac cgtggcagtt gctgtcaacg
    11701 ctattattgt aaaatcttga ggtaacgtaa atacttttga aataacattt tggtaactcg
    11761 gcctgaaatc atgaccccat ttagaaatac aatgcgcttc atcaaacgcg actaagtgaa
    11821 tctttatacg ctgaagcata tttaaaaaat atcggttttc aaatcgttct ggtgcaacat
    11881 acaaaaattg aatttctcca tttgataatg ctttttcaat acgttgttgc tctttttgag
    11941 tcaaactact atttaaaaaa gcagcttgaa ttcccatcgc ttttaattga tccacttgat
    12001 ctttcattaa tgatattagt ggacttatta caattgttgt accacctaac aataaacctg
    12061 gtacttgata gcatatagac ttacctccac cagttggtaa gacaccaagc acattacgat
    12121 ggtctaatac tttgctaata atttcttctt gtcctggtcg aaacgtttca tacccaaagt
    12181 aatgcgataa tgtttgttgc atcataaatt gaccccttat tgttgttctt taatttcttc
    12241 taactcactc catcttgtga tgtctaaatc atattgaatt tcaagttgtt ctttttcttc
    12301 gtttaattct ttaattttcc cataatctgc acttgcctca atcatgagca catcaatttc
    12361 ttccattctt acttccgctt gttctatgcg tttcatcaat tgttcatatt ctaatttttc
    12421 tttatatgat aaaccatttt tcttacgtac agttgtagaa gatttagatt gttgcttcaa
    12481 tgtggattta tttttatcta atgatttttt ataactttca taatcttcaa aagttccgat
    12541 aatcttttcc atctgaccat catgaataaa ccaatatgac tgtgcaactt tatttaagaa
    12601 gtagcgatca tggcttacgg taatcactgt accaccgaaa gtatgaatat aatcttcaag
    12661 tattgttaaa gtctcagtat ctaaatcatt tgtcggttca tctaacaaca gaacatttgg
    12721 ctggtgtacg agtagacgta ataaatacaa acgcttttgc tctccaccag ataatttata
    12781 aactttttta ccatgagttg cacttggaaa taaaaatcgt tcaagaagtt gtgtaataga
    12841 taccgaggtt ccatcttttt cttttgcgat ctcattttct tctcttaaat aatcaatcat
    12901 acgaatatct ctatccaggg tctcatctgt ttgcttaaaa taagctactt taaccgtctg
    12961 cccagtcttc aatttacctt cgaattgttg gtcttctcca ctcaaaatat ttaagagtgt
    13021 tgttttacca gctccatttg gcccaacaac accaatacgc tcaccttttt gaataatttc
    13081 cgtcagatgt tcaattaata ctttatcatt aatagccttt gataagtctt ctaattcgaa
    13141 cacttgctta cctaatcttg aataagcaag attcaattca cctttatcgt ctttatattg
    13201 ctggttaact tcattttcta ggtcattaaa tcgattaatt ctagcttgtt gctttgtagt
    13261 acgagcctta gctccagccc tcatccaagc aagttcttcc ttatataaag ctcgttgctt
    13321 ttgttgttgc ttttgaagtg ttacttctct ttcagcgcgc atttcaatat aagattcata
    13381 gttaccagga tatgacgcta acttacctct gtttagttca ataattctag tggaaacttc
    13441 atttaaaaaa tatcgatcat gggttacgaa taaaacagta tgaggatatt gcttcacata
    13501 attgatcaac cagctgattg attcgaagtc taaatggttc gtaggttcat ctaataacaa
    13561 taaatctggt tgttctatta atgttttagc aagtacaaca cgtttttgtt gtccgccgga
    13621 taattcttta atgtatttag tagtatcatg tatacctagt tttgagagga ttgttttaat
    13681 ttcagcgtta tagtcccaag catcatgttg atccatagca tcttgcgcat ccatcattcg
    13741 cttgaacaat ttatcacttt ggtcatccgc ataagcttgt actgcctgct catattgctt
    13801 gatgatgcgt aaagttgttg tatcagaact taatactgca tcaaaaactg tcatatcttc
    13861 attaaggtcc tgtttctgag acgaatatcg aattcgatat tgatttggat gcataacatt
    13921 ggctgtaaaa tcatcatcaa taccaccaat tacttttaac aacgtacttt tccctgtacc
    13981 atttatgcct actaaaccta ttttttcacc ttctgaaatt gataaatcta ggttatcgaa
    14041 tatagtctta tcggcataag atttatttaa atgttcaatt ttatatgctt ccatacccat
    14101 cttccttctt ctgttgaaat ctctacgtac ttaaatcagt tctatacatt atacaccttt
    14161 ttgaaatcaa aagaaatcga taccgttaag tgtcataatt taatatattg ttgcttcaac
    14221 tataaataaa acttgtta
//
LOCUS       DAKTLH010000064        14090 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.064, whole genome shotgun sequence.
ACCESSION   DAKTLH010000064 DAKTLH010000000
VERSION     DAKTLH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14090
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.064"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            5..424
                     /locus_tag="O0394_001553"
     CDS             5..424
                     /locus_tag="O0394_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0499633.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDIKSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            451..561
                     /locus_tag="O0394_001554"
     CDS             451..561
                     /locus_tag="O0394_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499634.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(631..4401)
                     /locus_tag="O0394_001555"
     CDS             complement(631..4401)
                     /locus_tag="O0394_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCY0499635.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKS
                     AAKPAALETQYKTAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYK
                     PQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTING
                     EISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASF
                     ARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSH
                     NYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTGKLTVAANNGV
                     AQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVK
                     EGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQI
                     DKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINA
                     KNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVI
                     YNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIY
                     LFGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNN
                     TGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNV
                     QNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVY
                     LYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYS
                     LKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVA
                     QIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISID
                     KINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNK
                     VVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQ
                     NTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(4629..5063)
                     /locus_tag="O0394_001556"
     CDS             complement(4629..5063)
                     /locus_tag="O0394_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0499636.1"
                     /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(5218..5688)
                     /locus_tag="O0394_001557"
     CDS             complement(5218..5688)
                     /locus_tag="O0394_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HCY0499637.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(5736..6953)
                     /locus_tag="O0394_001558"
     CDS             complement(5736..6953)
                     /locus_tag="O0394_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HCY0499638.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDNSLLRKEQNDTTDKDKTSNENSDSTNNSDSSNQQQPATDQNSNQNQGGTQQ
                     APQASNNQNGVVN"
     gene            7404..8597
                     /gene="fmtA"
                     /locus_tag="O0394_001559"
     CDS             7404..8597
                     /gene="fmtA"
                     /locus_tag="O0394_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HCY0499639.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETK
                     LSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRP
                     NILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKKYPDEYRYGFY
                     AKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
     gene            complement(9134..9412)
                     /gene="qoxD"
                     /locus_tag="O0394_001560"
     CDS             complement(9134..9412)
                     /gene="qoxD"
                     /locus_tag="O0394_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HCY0499640.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(9421..10026)
                     /gene="qoxC"
                     /locus_tag="O0394_001561"
     CDS             complement(9421..10026)
                     /gene="qoxC"
                     /locus_tag="O0394_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HCY0499641.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(10016..12004)
                     /gene="qoxB"
                     /locus_tag="O0394_001562"
     CDS             complement(10016..12004)
                     /gene="qoxB"
                     /locus_tag="O0394_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HCY0499642.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(12004..13104)
                     /gene="qoxA"
                     /locus_tag="O0394_001563"
     CDS             complement(12004..13104)
                     /gene="qoxA"
                     /locus_tag="O0394_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HCY0499643.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            13406..13528
                     /locus_tag="O0394_001564"
     CDS             13406..13528
                     /locus_tag="O0394_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499644.1"
                     /translation="MIERKRKHFNKEFAKSNLSLNNEKWKFVCSKSKHFFKKSS"
     gene            complement(13676..13993)
                     /locus_tag="O0394_001565"
     CDS             complement(13676..13993)
                     /locus_tag="O0394_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HCY0499645.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
BASE COUNT         4579 a         2237 c         2372 g         4902 t
ORIGIN      
        1 caacatgtat aaacaacttg aaaaacttat tacactgact aacaatgact taaacttagt
       61 gaatagaaga tttggacaac gcacggatat caaatctgaa cagctagaac ttctccgtat
      121 tttatttaat tacgatcgct tatcacagta tgatttgacg atgaagatta gcagggaaca
      181 atctatagtt tcaaggtgga ttaagaaatt agttttgaaa gggtacatca caagtcaaca
      241 atctagcgaa gatttaagat gtaaagaatt aattttgact gatcaagcac gtacattaat
      301 ttcacaaata aataatgcac gttgcgaatt gattgaagca agatgtcaat gtttatcgga
      361 agtcgaatta gacaatttaa atcaattact tgataagtta aatcaacgac gcatatcgtt
      421 gtaacacgac aatagcaaac acataattta gtgtcgttgt gcacacaaca aaaattgaat
      481 catgaattac aagcaaaagt agcggtgatt gttaaaattg atggtaaaca atcaccgcta
      541 tttttgcttg tgtatgtata aaaaagggat caaaggtcat cccccatgat tgatagtggg
      601 ggatgacttt tgatcctatg ttcatgttgc ttatttatat tgtgggatgt cgaagtattt
      661 gccgacttcg ccaattttat catagtagcc tttgatgatt ttagcattga tgttagccca
      721 atctacatct gtagcatatt ggtgtgttcc tggatgtgca ggattccatc tcattttgta
      781 aagtgtattt tgaccagctt ttacatatga gttgccgatg aatttagcac caccaacgat
      841 tgcttttgat actgtgtccc aaccagcttg tttagcatat ttaatacctt cacgtaaagg
      901 atcgttatca tatgcagcaa taccaaatac gttatggtat ttcgtgtttg agttagttac
      961 aactttgttg ttcactacat ctgcaccttt cgctaattga gaagtaccgt tacctgtttc
     1021 taatagggca tgtgagataa gataaacttc attaatgcca tacatttgag cagctttgtt
     1081 aaatgcagca ccttggtttt ctaatacacc tttacctttt aagaattgat taattttatc
     1141 aatagaaata ttttgtggtt ggtctaagcg taagaattga tattttaatg ctggatcttg
     1201 agctaaacgc ttcgtatcca ttgcatgctt aacatcatta aagttagcat ctgtccactt
     1261 acctggtaca cgttgtactt gtggtttata ttgtaaacca gcttgtattt gagcaacttg
     1321 gtttaatgtc atacctgttt gattatactt aattaattct ttagctaaat cagttgattt
     1381 aatccatgct aatttaccgt tagataattt accatagtac caagtttgtc cattaatgac
     1441 ttgttcttta acaactgcga atggttgttc attaaatgct tttaatgagt atttagctgt
     1501 atcagaattt ggtgttacat agtaataacc attaccattt ttaattacat aagtgtagtt
     1561 ataatctttg gcagctgatg tagttggttt cacagcggtt ggtgcagtta aatcttttgc
     1621 atttacccaa ccagtgcggt tattaatagt accgtataaa taaacatctt tgcctacaga
     1681 tacttgtttc gttgcattaa atgtaccttg agcaatgtta ttgcctgtta aaatgacttg
     1741 gtttttagta ccccaaggaa ccattgataa gccgttattt gatttattaa cagtatattt
     1801 ttgagtcgtt ttaacttctt tgcctaagtt ttgaacattt aagtctttta cattgaacca
     1861 acctaatggg atgttatggc ttgtattgtt taataataca tacgtttcat taccatgagc
     1921 acgctctttt gttacataga acgtacggtc tgcatatttc gcaccgtttt tcgctgtttt
     1981 ttcataaaca gaagcacgaa taccagtgtt gtttggttta acttgagcaa tcttgctaac
     2041 tgtttgagtc gtttgtggtt tagtaacagt ataagctttt acagctgttt ttggttgtgc
     2101 tactgctttt ttaggtgcag caggtacagc taaatatgct ttacttaccc aaccagattt
     2161 accatttaca gttccaaata aatagataga tttatcaatt tgttgttgct tagtcgcttt
     2221 aaaagtttgg ttacctgtac cagaaactgc accagcttct tgtttataag tgccccaagg
     2281 tactgaatat aatttagtgc ctggttttac tgtatatgtt tgcattacat ttacaggtga
     2341 ttttgcattg ttataaataa cgtcaccttg tttaacccaa ccaattaaag ttggactatt
     2401 gtaatcttta actaagtaga atttgtttcc acctaaactt gcttcttttg ttacagcaaa
     2461 tgttttttga acttctttcg ttggcttacc agttttgtca taaactgtag tgaataagcc
     2521 attgttttta gcattaattt gagcaacacc gtttaatgat gaaactgtta atttattatt
     2581 tgttgtaggt gttgatggct taggtgttgg tgtaggcgta ggtttagcag tatcaactaa
     2641 atatgcttta cttacccaac cagatttacc attcacagag ccatataaat aaattgattt
     2701 atcaatttgt tgttgctttg aagccttaaa tgtttggttt ccagagccag acacactacc
     2761 agcaacttgt ttagatgtac cccaaggtac tgtataaagt ttcgtaccag gtttgattga
     2821 atatgattga tttacattta caggtgattt agctgtgttg taaaccacat cgccttcttt
     2881 aacccaacca aatttattac cagaattgta atcttgaaca agatagaatt tttgattacc
     2941 taatgtagct gttttagata cagcaaatgt tttttgaact tcattagttg ctttaccagt
     3001 tttgtcgtat acagtagtat ataaaccact atttgttggt ttgatttgtg cgacaccatt
     3061 gtttgcagca actgttaatt taccagttga tggtttcgac ggtgttgttg gttttgatgg
     3121 tgtagtaggg gtagttgtag attgcgtacc ccatggcgcc actttaccca tttttattaa
     3181 atatttttca ttaattaagt catataattg atcataacta taattatgac ttcttaaata
     3241 tccatgtgga tcggcatggt cagtaccacc taaatattta cttacagcgt agtgagtcca
     3301 tactgtacca tttccatcat actcagcact gtctggtttt aaaccataat attgtaattg
     3361 tgtagctgca tagtcagcat agttattcat tgaacgtgca aatgaagcat agtcgtgtgt
     3421 gtgtacgatt tcaacattga tgaatctagg gttaccgact gcaccgacac cccaagataa
     3481 gtaatccgtt ggtgctgttt cgattatacg atccccatca acaaatgcat gtacgaatgc
     3541 gttttgatag ttatttttca tataactaat ttcaccattt atcgtcgaac gatcattagc
     3601 tgtatcatga actacgatac cttcaggacg acctacgccg ttacggtatg cgtatttagg
     3661 gaagtaagat gtataatctt cttcaatttt aggtgctttt aagttatttt tacgaatgta
     3721 atcgttaatt gaagagttta cttgtggttt atattttggc aaactcgttt ttggtgttgc
     3781 agcaactgat cttggttgtg ctgaagcgct aaaagtagtt actttaggtg tcgcttcagt
     3841 tttagcttta ggtgctgatg tagttgcagc tttaggtgct gcggttttat attgcgtttc
     3901 aagagctgca ggtttagcag ctgatttaat taattctgga ttaatttgat tttctgaatt
     3961 atcatcttca tcatcaacta aactataacc agcatttgta acattagtgt tagttttagg
     4021 tgctgtagtg cttgttgact ttgcaacagg ctgcgtatta tttgtagtcg ctgattgatt
     4081 agcacgagtg tcaccattta cttgtgcagt atcaactttt tgacttactt gagcattgcc
     4141 tgttttgtta tttgctgttt ttggttggac aatagcaggg tcttgatata cttgagtgcc
     4201 agaaatgttt tgcgttggat tttttacctc agcttttgct tgttcagtag ttgctttaac
     4261 tttattacta tctaaaacgt ttttattagt agtttgatct tgtgtcgtct cagctgcttg
     4321 aacttgatgt gcagtgactg ctgaacctac aagcgttaat gcaaccattg atggtagttt
     4381 gtaattgaat tttttcgcca ttctatttat tactcctaac atttattaat tattactaac
     4441 attatagtac ctgttttata tacctgtgcg tatttaacca atttgtaata tactcttaat
     4501 ttacattttc actgtaaaaa aatgaaaaaa attttgaatc tcaatggtga cgtgacaaat
     4561 caaatagaat tgatgtcaat ttaagaattg gtttattaca aatgtttcta aaatagatac
     4621 aacttttttt aattaagcga ggttaacttt tttgtcattt caatatgctc gatgccttcc
     4681 tcaagaaata tattacctct cattttaaag tttaaacttt cataaaatgg gatagcatga
     4741 cattgggcat tcatagtagc tacgtaaaaa ccttcatctt tagctaatga ttctacagct
     4801 tgcataagca ttctacccat tccttgtcca cgatgtgatt tcatcacagc tactcgttct
     4861 attttgacag ttgtttcatt aatagggcgt attcgagcag tggcaactgg ctgtccatta
     4921 tcatatccaa tgaggtgaat agattcagat tcatattcat caatttcact ttcctcaggg
     4981 acgccttgtt cttctacaaa cactttcttt cttatataga agcaatcttc taacatcttt
     5041 tgattgttta cttttgaaaa cataaaattc ctccacatcc taacaaaatc atttacattt
     5101 aatttactaa taataacata gaataacgat tgttattttc ctcaaaaata attagacata
     5161 aaaaagctat aaagatgatt gttgtgaatc atctttatag ctaattaatt attaatgtta
     5221 tagtgcttct tttctaataa ttgctgtata ttgccaaaag attcgcattt gagcaatatt
     5281 ccaattattc attcccattg taaatcctga aggcttaaat atattcacat cagttacttc
     5341 taaagctgct gcaactaaat attgtaatgg aatgctaagt gatttcattg tttcggtaat
     5401 gccattttca tcatataccc atgaaaatgg taactctgat tcgaaaacat caacactatt
     5461 gaaaatttca ggtagggcta aaatataaca tgcaccatct acaataggat tgtcgatact
     5521 atttttgtaa taaattagga gtgcttcata gttttctcga tgctcatgat tgacgtaaaa
     5581 aaattcactt ctaaaatgag ccatatcttg gctatgaatg atttgttgtt ctaacatatt
     5641 aaacatgcca tttatatttg caatcttttc gtatgttttt ctactcatgt atgatgctcc
     5701 tttcataaat agtaaatgtt aatcgtcata taatattaat ttacaacacc attttggtta
     5761 tttgaagctt gtggcgcttg ttgtgtgcca ccttgatttt gatttgagtt ttgatctgta
     5821 gcaggttgtt gttgattgct ggaatcactg ttattagttg aatcactgtt ctcgttagat
     5881 gtcttatctt tatctgtcgt atcattttgt tcttttctca ataaactatt atctaaaggc
     5941 gttaatggta ttaatgaacc ataatgatta atgacacgtt gatctaagaa atcattttta
     6001 tcattaatag gtgataattc taagtcttta cgaagtaagt ttgcatattt ttgaatgctt
     6061 tcaacacttg gatgataata gtaaatacca tttaacatat catctttacc ttttaattgc
     6121 gcagttttaa tttcaacatc atttgttaag tacatttttg ctaaagcttt aatttcagag
     6181 ttagttaaat tatgctttgc atttttacct acaatttgaa tcacgttatc aagtttatca
     6241 atagaatcaa cttcctgtgc tttttggaat aaaatcttaa ttaattccat ttgacgttgt
     6301 ccacgtttta agtctgaatc atgatgtcta gttctagcaa ctgctaaagc ctcatcacca
     6361 tttaattttt ggtacccttt tttaatttta atcttaccag tatcatctgt gttaggttca
     6421 tttaagtcgt atggcacatc atagtatatg ccaccaagct catttacagc ctcgacaaat
     6481 gctttcatat tgactctcac ataataatca acaggtacat tcatggtagc ttctaccgaa
     6541 tccattgcgg caattggacc accatatgca tgtgcatggg taatcttatc gtaatagcca
     6601 actttaggaa tgtagctgat agtatcacgt ggaatactaa gcattctaat ttgatgtttt
     6661 gattgattaa aagtagttaa aatcatagcg tctgatctag agtgttcagc atcctgtcct
     6721 ttttttcttc ttccatcgtt atcatcgata cctaagaaaa gaatagagat aggttgttct
     6781 tcgggattga ctttattatc tcttaagttg gattgacgat tagcattttt gctgtcttga
     6841 gaagattcga atgcatcttg ggacgtttta aaaagtaacg tagcgaagac tattggaaca
     6901 acaatgagaa ccaatgctag aaggatcaaa aagtatttta aaaatttatt catgattgat
     6961 gctcctatat taaatttttg ttaaatcata atcataattt taaaatataa ttagttgaac
     7021 gatatttata caacttcatt actaatacat tatagcctta aattgtagta ctttatagta
     7081 aaaaatacaa atgttatgtc aaagttcctt gtaatttgaa tgttttgtta aaatcaaaaa
     7141 gcatttttaa aaatataatg cgactttagt atgatgtctt aaaaaattta aaggcgtaat
     7201 ataaattcga gtgatttata caaagtgata aggtataatt aattttgttg tatagataac
     7261 atattagaaa aacttattac tatgtagcgt aaagcataaa tgaatgataa gttaattttt
     7321 gtaagttgta ttattaggca tctaggttca aatttttaac gatttaaata tataaaatga
     7381 atacattgtg aggcaattat caaatgaaat ttaataaagt aaaactagtt atacatgcgt
     7441 gtgtactatt atttatcatt atttctatag cgttaatttt tcatcgatta cagacgaaga
     7501 cacattctat agacccaata cataaggaaa caaaattatc agacaatgaa aaatatttag
     7561 tggatcgtaa taaggaaaag gttgcgccgt ctaaactaaa agaggtatat aatagcaagg
     7621 atcctaaata taagaaaatt gacaagtatt tacaaagttc attatttaac ggttcagtag
     7681 ctatatatga aaatggcaaa ttgaaaatga gtaaaggtta tggatatcaa gattttgaaa
     7741 aaggtattaa aaacacaccg aatacgatgt ttttaatagg ttcagctcaa aaattttcaa
     7801 cagggttact gttaaaacag ttagaagaag aacataaaat aaatatcaat gatccagtaa
     7861 gtaaatacct tccatggttt aaaacatcta agcctatccc attgaaagat ttaatgttgc
     7921 atcaaagtgg attatataaa tataaatcct caaaagatta taaaaattta gatcaagcag
     7981 ttaaagcgat tcaaaaacgt ggtattgatc ctaagaaata caaaaagcat atgtataacg
     8041 atgggaatta tttagtactt gcgaaagtaa ttgaagaagt tacaggtaaa tcttatgctg
     8101 agaattatta tacaaaaata ggagatcctt taaaacttca gcacacagca ttttatgatg
     8161 aacaaccttt taaaaaatat ctagcaaaag gttatgctta taatagtaca ggactttcat
     8221 tcttaagacc taatattttg gaccaatact atggtgcagg taatttatat atgacaccaa
     8281 cagatatggg taaattaatt actcaaatac aacaatataa attattcagt cctaaaataa
     8341 ccaatccatt attacatgag tttggtacga aaaagtatcc agatgaatat cgatatggtt
     8401 tctatgctaa gccaacattg aatagactta acgggggatt ctttggacaa gtctttactg
     8461 tttactataa tgataagtat gtagttgtac ttgcattaaa tgtaaaagga aacaatgaag
     8521 ttcgaatcaa acatatttat aatgatattt taaaacaaaa taaaccttac aatacgaagg
     8581 gtgttattgt tcaataatta atatagaaga tataacatgt atatggcatt aaggcatcga
     8641 ccttatctga ccagtatacg agttatatct tcttttttat agtggtaaaa agtttaaagt
     8701 ataaggttga agaaggatga gtttaaaaat atgtgttaac tgataaaagg ggaaatcatt
     8761 tggtgagttg gcatcagact aaaatgaatg aagacgaata cgttggtcca tgcgtggtgt
     8821 gaatgttttc taaatctaac tggaaatgat aatttaataa tgtacacttt tgattgttta
     8881 aacatgtaca atttttaaaa acctagggat aattttattg acgtgaacaa cattgagatt
     8941 atctttagac ataaatatgg attttgaagt tactatattt attagttgtg gttgttaatg
     9001 tattaattga attgattata atgattgtct tataagatat agaaggggac ttaaccattt
     9061 aaaaattgaa ggaaaataaa aaccagctaa caaaagtgtt agctggttaa actttacatt
     9121 attaaaatgt tgtttataag tgtgaagagt gaccgccttg cataacccaa tatgttccga
     9181 caacgaaaca aagtgtaatt acaagagcaa agataacttt gaatgtttgt aaacgtccat
     9241 ctttaccttc agttaaatgc atgaacatta ataattgaag tcctgcttgg acgaatgcaa
     9301 agccaaagat aattgtcaac ttcgcgtgga atgttaatga cgtgtatagt gttacgtaaa
     9361 ctgctaaaag cgttaatacg atagatgcga taaatcctac agtatgtttc attattgtac
     9421 tcatccgcta tacaccatcc ctatcatata tacggcagta aagatgaaaa cccaaacaac
     9481 atctaagaag tgccagtata aacttactat aaataatttt ggcgcattat atttgtctaa
     9541 tccgcgtcgt tggatttgga ttaataaaca aatggcccaa acgataccta gcgatacgtg
     9601 acaaccatgc gttcctaata ggataaagaa actagaccag taagaaccaa ttgttgggtt
     9661 aacgccttct gatgcatagt gtgcgaattc ataaatttcg aatccaacaa agactaaacc
     9721 taaaagtaac gtaatgatca tccaaaacat cattaacttt tgtttttctt ggcgcatgta
     9781 gtaaatagca ataccacatg tgtaagaact gaataataat gcaaacgtca ttattaaaac
     9841 aagaggcaat tcaaataact cagtagtcat tttacctgca taatcgccac catgttgcaa
     9901 agttaatagt gtcgcaaata gggtaccgaa taacgcaaat tcggctgtaa tgaaaatcca
     9961 aaagccaagc ttatttaatt cgccttcatg tgtgcgtgaa tcaatagtgt ttgtatcatg
    10021 actcatgact tacagcctcc ctttctttaa tacgcgcttc tcttaatcta gcttcagttt
    10081 ctgcaacttc agcagcaggg atatgatatc cgtgatcgat ttggaaactg cgataaatca
    10141 tagtaccaaa aataccgaat aaacaaatta atgctggaat tacagtttcg aaaattaaga
    10201 agaaaccgcc gatagtcata aagataccaa tccagaatcc aacaggagta ttgtttggca
    10261 tatgaatgtc tttgtaatta tggttgtcta agtaatgacg accatgttct ttcatatcaa
    10321 caaatgtgtc gtagtcattc caatctggtg taatggcaaa gttgtattta ggtggaattg
    10381 ctgatgcagt agtccactct agagtacgac caaggccatc ccagttatct ccagttgctt
    10441 cacgtggaga tttgaagtga ctgtatacga tactaacaac aaggaataag aatccgattg
    10501 ccattaataa tgcaccgata gttgaaatta agtttaataa gaaccaacca tctgatggca
    10561 tataagtgta taaacgacgt ggcataccat ctaatccaag aatgaattgt ggtaagaaac
    10621 aaacgttaaa tccgatcatg aagaaccaga agcaccattt gtttaatgtt tcgtttaatt
    10681 tgtaacccat catctttgga taccagaaga ttaaaccagc taagcaggca aatacaacac
    10741 cagtaaccaa tgtatagtgg aagtgagcta ctaagaagta cgtattgtga tattgatagt
    10801 cagctgatgc cattgctaac attacacccg taacaccacc taaaaggaag ttagggataa
    10861 atgctaatga gaatagcatt ggtgactcaa atgtaatacg tcctttatat aatgttaata
    10921 accagttaaa caatttcaca ccagttggaa taccaatcag cattgttgaa attgagaaga
    10981 atgagttgat taacgcacca ttacccattg tgaagaaatg gtgaacccaa actaagaaac
    11041 taaggaacgc gatgccggca gttgcccata ccatactttg atgtccgaat aaacgcttac
    11101 gagcgaatgt cgggataatt tctgagtaaa taccaaatgc tggaaggata acgatataaa
    11161 cttcagggtg cccccatacc cagaagaagt tagcccaaag cattggcata ccgccatgtg
    11221 caactgtgaa gaatgctgtg tcaaatattc tatcagttgt cattaatgct aacgctactg
    11281 ttaaaggagg gaaagcaaga ataacaatta atgtagtaat aaatgttgtt actgtaaaca
    11341 ttggcatttg cataaacttc atagttggtg ttttacatct taaaattgtt acaaagaagt
    11401 tgatacctgt agctaaggta ccaagccctg aaatttgtat agctattaag taatagttaa
    11461 cacccggacc aggactgaat tcacctgcta gtggcgcata gtttgtccaa ccagctgctg
    11521 gtgaaccacc aataataaat gacaggttga ataaaatcat acctgcaaag aatagccaga
    11581 aacttacgtt gtttaatact gggaatgcaa catcacgtgc tccaatttgt aatggaacaa
    11641 cgatattcca taaaccaaag ataaatggca ttgccatgaa gataatcatg attacaccat
    11701 gtgtactaaa aatttcgtta tagtggttag attctaaaaa tttgttatca ggtactgtta
    11761 attgcgcacg aataagtaac gcatcaatac caccacggac gaacattaat acggcacaga
    11821 ttaaatacat aataccgatt ttcttatggt ctacagatgt gaaccattct ttgtaaagat
    11881 atttccataa tttaaagtaa gtaattactg cgattaaacc aataactaag aatggggcac
    11941 caatttgtgc cattgtaatc atccagttac ctttaactag taattgatcc catggaaaat
    12001 tcattaatgt ccacctccat gatcatcatt gtcttgatct tgcgcatctt ttgaaatttt
    12061 cttcatttct ttcgcatttt tcgattcatc tttcttgaac tcattgttat atggttcgtc
    12121 atttccaaga atcatcaact tcataccatg tcgtttatag ttcgcatttg taatttgagc
    12181 tttacgagca ggtattaatg gtttgtctga tacatcttta aacatatttt cttcactagt
    12241 gaagtttgga tcttttaatt cgaaattgaa acgtttatat gcatagaaga tgtattctgg
    12301 atcggctgct ggatcaacaa acgccatatg tgtaccatta aattctaaag ctttattagg
    12361 tgtgcttggt aataattgtt tatcaaatgt atcttgatct aacgttttct tacctttaac
    12421 ttctttcacc catttgtcgt agtctttttg actaacggca tttactttaa atgtttgacg
    12481 tgagaatcct tcaccattga agttagagtt acgacctctg aacgtaccag tttgagatgc
    12541 ttctaacgtc caattcattg tcatgccagt catggcatat ttttgaccac ctaattgtgg
    12601 aatccagaaa cttgtcattg tatccatagc ttgaagctta aatacaacag gacgatcttt
    12661 agggattgtt aatgtattaa cagtctctat atgttcatct ggataagcaa agaaccattt
    12721 gtatcctgca cttactgcat atacaaccat tggatctttc tcactcttcg gtggtttttc
    12781 gtaatcgtat aaagttttaa ctgtaggaat agctaaagca gcaacgatta tgataggtat
    12841 tacaaaccat attgtttcaa tgatggcatt atggtgcatc ttaccagatt cggcattctt
    12901 attataacta tacttgtaaa taaaaatggc gaacatgcca agtacaacga aacaaataac
    12961 aagcatgaag acgattgaat aaagaatcaa gaacttctga ctacttgcta ctggcccttt
    13021 tgcgttgaaa atttctatat ttgaacaacc actaagtaaa attagtgtgc caaataatag
    13081 aagcaaagac ttaaattttg acactttttt gacctcctaa tactacaaat gtagggctta
    13141 acattaattt taagttatta cacaatattt acaagggctt atgggaaaaa aattaataaa
    13201 attgtatcaa aaatgttgat aaatcaaggt gtgacgtggg ttcacacatt tgttaaaatt
    13261 atgtgtacat tttgtgacta atagcgtttt tgaaaaaatt gcttcatatt taaaaggaaa
    13321 gttgagaata gctttcaatt aaaggttaat tgaatgaaaa taaaaaaagt ttgaaattgt
    13381 ctaataaatt gaaaatgatg gctagttgat tgaaaggaag cgcaaacatt ttaataagga
    13441 atttgccaaa agtaatttat cgttaaacaa tgaaaagtgg aaatttgttt gttcaaaatc
    13501 aaaacatttt tttaaaaaga gcagttaagt tttaataatt tgataggtag tcctttggta
    13561 tacagaaaaa atacaatatt tgggttaaag ttgcagatga agttagaata acaaataaaa
    13621 tggtagcaaa acatgttcgt ttgctaccat tttatttgca gtatagtgat gagggctatt
    13681 taacctcaat atacctagtg gaaattttaa ttgcaccatc tgaatcagtg acatgatatt
    13741 taatttgata tttaccagat ttagatgtat caatttggcc atcgacttta attttatcgg
    13801 ttaaatctcc atcttcttta tcaaatgcac ttatgccgtt tagaaggtta taatcttgac
    13861 ctttcttaat aacgatatca ttagcgcctt taatttgtgg tgtagtattc gtcgttgcat
    13921 ctgcatttaa atttggtgtt actagtgtag cagaaacacc gagggctgat aatgactgta
    13981 atagtttatt cataatgtaa cctccgaatg ataagtcatt tctgagttag ctttactata
    14041 ccttaattta tattatttac acatcatact atagtcctaa tcttacccta
//
LOCUS       DAKTLH010000065        14019 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.065, whole genome shotgun sequence.
ACCESSION   DAKTLH010000065 DAKTLH010000000
VERSION     DAKTLH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14019
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.065"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            157..1005
                     /locus_tag="O0394_001566"
     CDS             157..1005
                     /locus_tag="O0394_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCY0499646.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEIREVTKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            1034..1588
                     /locus_tag="O0394_001567"
     CDS             1034..1588
                     /locus_tag="O0394_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HCY0499647.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGRFSRKSMVYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            1641..3353
                     /locus_tag="O0394_001568"
     CDS             1641..3353
                     /locus_tag="O0394_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0499648.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQNINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLKDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMS
                     EHVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIAALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLVLNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            3350..4183
                     /locus_tag="O0394_001569"
     CDS             3350..4183
                     /locus_tag="O0394_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCY0499649.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISASSILLVKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELV
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPW
                     H"
     gene            4230..4727
                     /locus_tag="O0394_001570"
     CDS             4230..4727
                     /locus_tag="O0394_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0499650.1"
                     /translation="MINTERLNLMIPSSSHLIELYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWRNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLKFKRDDTMDDIINQGD
                     IVFYK"
     gene            4845..5969
                     /locus_tag="O0394_001571"
     CDS             4845..5969
                     /locus_tag="O0394_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCY0499651.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRRYFKGNRDYEE"
     gene            6274..7092
                     /locus_tag="O0394_001572"
     CDS             6274..7092
                     /locus_tag="O0394_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HCY0499652.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHK
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            7110..7724
                     /locus_tag="O0394_001573"
     CDS             7110..7724
                     /locus_tag="O0394_001573"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCY0499653.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELAGVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            7717..8967
                     /locus_tag="O0394_001574"
     CDS             7717..8967
                     /locus_tag="O0394_001574"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCY0499654.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVDQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDETADLDAIVYDVFAQAEHDELARTYAISEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            8983..9996
                     /locus_tag="O0394_001575"
     CDS             8983..9996
                     /locus_tag="O0394_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HCY0499655.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGTAYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLN
                     VFTLNIATYIFRHREETRQFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            9965..10543
                     /gene="hisB"
                     /locus_tag="O0394_001576"
     CDS             9965..10543
                     /gene="hisB"
                     /locus_tag="O0394_001576"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HCY0499656.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            10540..11118
                     /gene="hisH"
                     /locus_tag="O0394_001577"
     CDS             10540..11118
                     /gene="hisH"
                     /locus_tag="O0394_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCY0499657.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            11111..11815
                     /locus_tag="O0394_001578"
     CDS             11111..11815
                     /locus_tag="O0394_001578"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCY0499658.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRRLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            11812..12570
                     /gene="hisF"
                     /locus_tag="O0394_001579"
     CDS             11812..12570
                     /gene="hisF"
                     /locus_tag="O0394_001579"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCY0499659.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQ
                     TDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR"
     gene            12567..13199
                     /locus_tag="O0394_001580"
     CDS             12567..13199
                     /locus_tag="O0394_001580"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCY0499660.1"
                     /translation="MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            13392..13571
                     /locus_tag="O0394_001581"
     CDS             13392..13571
                     /locus_tag="O0394_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499661.1"
                     /translation="MKSKLSNSGIDVTVKDVEKYMNRYNEVMKGKNGEKAKELCLSLL
                     PINIIVVFTFFVFIL"
BASE COUNT         5005 a         1986 c         2712 g         4316 t
ORIGIN      
        1 gataaatctg actattatta tcatttttat agtcactact attgactttg catcaagtga
       61 ctcgtactat tttaatataa ttctgaaaat tcaaaatata gaacattaaa tatgtagata
      121 atattttttt gaagttttaa aaaaaggagg cattttatgg gaaattattt ggtattgcaa
      181 tcagattttg gacttagtga tggcgctgta agtgcaatgt atggagttgc atatactgta
      241 agtgatgata tacgtgttga aaacttaacg catgatatac caccgtatga catttgggta
      301 gcgtcatatc gtttatatca aactgtaaaa tactggccta aaggcacagt ttttgtatca
      361 gtggtagacc cgggtgtagg tagtgatagg cggagcattg cttgcttaac atattcaggt
      421 cattacatta ttacacctga taatggttct ttaagtcata ttaaacatta cgaaggtatt
      481 aaaaaagtta tcgaaattga tgaagtgaaa agccgattgc ctcattcgga agaaagtcat
      541 acttttcatg gtagagatgt ttatgcatac aatggtgcga gattagctgc aaataagata
      601 gcgtttgaac gtctcggtca ggcaattaat gttgatagta ttgaggcatt agaaataaga
      661 gaagtgacaa aaaatgagga ttctgttaca ggaagcattg atattttaga tatcagattt
      721 gggtcattat ggacaaatat tccattggca ttcttaaaat ccttggaaat tgttcatggt
      781 aataatttgg tggtaacaat ttatcatcaa gataagaaag tctatcaaaa tattattaaa
      841 tttgcgcgat cgtttgcaga tgttaatgtg ggtgaacctc ttgtgtatgt taactcgcta
      901 gtaaatattg gtgtagcagt caatcaagac tcattttcag atttatatca tattggtact
      961 ggaaatgatt ggacgattca tttatctaaa gcaccatcaa tataatgaat tttttatgta
     1021 aggagtaatg aagatgaaaa aacaagatat ttcagttaaa actgttgttg caattggtat
     1081 aggagcagcg gtatttgtaa ttttagggcg ttttgttgta ataccaacag gttttcctaa
     1141 tacgaatata gaaacatcat atgcattttt agcattaata tctgcaatat ttggaccttt
     1201 tgctggttta atgactggat tagttggaca tgctattaaa gattttacga catacggtag
     1261 tgcctggtgg agttgggtta tttgctcggg aattataggc tgtttatatg gctggattgg
     1321 actaaaatta aatctttctt caggccgatt ttctaggaaa tcgatggttt attttaatat
     1381 cggtcaaatt attgcgaata ttatttgctg ggcacttatt gcaccaacat tagatatttt
     1441 gatttataac gaaccagcta acaaggttta tacacaaggt gttatctctg cagtattaaa
     1501 tattatttca gttggcatta ttgggacaat attattaaaa gcatatgctt catctcaaat
     1561 taaaaaaggt agtttacgta aggaatagtt attttgttga atcagatatg taaatgaata
     1621 tagaaaggta atgatatatc atgactgaac caattatctc gttcaaagac tttagttttc
     1681 aatatcatag tcaagcaaca cctacattac agaatataaa tgttgatatt tatccaggag
     1741 aaaaagtatt agtagttggt gcttcgggta gtggtaaatc gacttttgca aattgcataa
     1801 acggattaat tccatttaaa actaaaggta acataactgg ggaactatat ataaataatc
     1861 aagatgcaac cgttagttgt ttacatgata gatctaatgt tgttggtaca gttttaaaag
     1921 atacagatgg acagttcata ggcttaacag cagctgaaga tatggccttt ttattagaaa
     1981 ataattgtgt tgaacaagat gatatgaaga aaaatgtaag ttattgggct gaaaaagttg
     2041 gcatgataga acatttaaat caccgaccgc aagatttatc tggaggtcaa aaacaacgcg
     2101 tttcattagg tggtatatta atccatcgta cgcctatttt aatattggat gagccactgg
     2161 ccaatttaga tcctgcgaca ggacatgaaa cgctgagatt gttaaacaat attcatgaag
     2221 aaacaaagtc aacgatgatt attgtcgaac atcgattaga agaatcatta gatgatacgt
     2281 ttgatagagt tcttcttttt aaagatggaa aaatcatcgc taatacaacg cctagtgatt
     2341 tgctaaaatc atctaagctt aaagaagcag gcatacgtga accattatac tgtacagcgc
     2401 ttaaatatgc agaagtagat gtcgaatcca ttgataactt agcgaattta cgtgacgttt
     2461 gtatgagtga acatgttaaa tttaaagtga aaaaatggat agatgaaaca tctgcaaata
     2521 atgataataa gtacaaatct gaaccgctat tagaattaaa tgaggtatgt gttcaatata
     2581 gtgactatag taatagtgta cttaataatg ttcaattaaa tgtttataga agagaaatgc
     2641 ttagtatagt tggtcacaat ggtgccggca aatcaacact tgctaaagca atttgtggat
     2701 ttttagatat aacaggtaat atccaatttt gtaatagggg atttaatcaa ttgtcaatta
     2761 gcgaacgatc tgaattcgta ggttatgtga tgcaaaatcc aaatcatatg atttctgaaa
     2821 aaatgattta cgatgaagta gcattagggt taagagcacg aggtatgaaa gaatcagata
     2881 taaagatacg agtagagaat gtgctcaaaa tatgcggact ttatgcattt cggaattggc
     2941 caattgcagc tttaagctat ggtcaaaaaa agagggtcac tatagcatct gttttagtct
     3001 taaatccgga aataatcata ttggatgaac cgactgctgg tcaagatttc tatcattata
     3061 atgagataat gtcattttta attgaactaa acagacaggg gaagacgatt attatgatta
     3121 cgcatgatat gcatttattg tctgagtata gttcaagaac agttgtatta tcaaaaggtc
     3181 aagtcgttgc tgataccacg ccagtattgg ttttaaatga taaaaaaatc tgtgagattg
     3241 catcattgag acaaacatcg ctatttgaaa tggccgaata tatagggatt agcgagccac
     3301 agaaattagt acaattattt attaaccatg ataggaaggt gagacgccaa tgaatcaata
     3361 taatactata ggttttcacc cgggaaatag tcgtattcat caattaaatg cgactgttaa
     3421 acttttattc ttattagttg tttctatttc tgcaatggtg acttatgaca caagatattt
     3481 aattttaatt agtgcttcat ctattttatt ggtcaaatat gctcatattg aatggaaaca
     3541 agttcgcttt gttgttaaat tcattctgtt tttcacaata ttaaatatta ttgccgtgta
     3601 catatttgac cctgaatatg gtgtgaagat ttataatcag cgtacagagt tagtcaatgg
     3661 tattggtcga tttacgctaa catcacagga attattctat ctttttaatc taatattaaa
     3721 atatattagt acagttcctt tagcgttaat atttttattc acaacgaatc cgagtcattt
     3781 tgctgcaagt ttaaatcagc taggtgtgaa ttataaaatc agttacgcag tctcactagc
     3841 attaaggtat attccagata ttcaagaaac atattttaat atttcacaag cgcaacaagc
     3901 aagaggatat gataattcga aaaaagcaaa atttactagc cgtgttaaag gtatcaaacg
     3961 cattgtgtta cctttaatat tttctagtat cgaaagaatt gacactatta gtactgctat
     4021 ggagttaaga caattcggac agtataaaag gagaacctgg tacgtcaaaa aacaattaaa
     4081 aaaagatgat tatgttgttt tgtgtttgac gttaatactt ctgatgttag tagttacatt
     4141 attcttttta aataatagtc gatatttcaa cccgtggcat tagtattcat ataaatagtc
     4201 tttaaataga aataggaggg agacatttaa tgataaatac tgaaagatta aatttaatga
     4261 ttccaagttc ctcgcattta attgaacttt ataatatttg tagtcatcca caagcaaata
     4321 tatacactcc caaaggttta cataattcca aattagacac acaacggtgg attgaaaaat
     4381 ggcgaaacca ttggcaacaa tatcaatttg gttactttgt attggtaaaa aaaatagatt
     4441 gtagtgttat tggtatttgt ggatatgaat atcgacaatt aaagcaagaa acagtattaa
     4501 atttatttta taaattacat ccaagttttg aaggacaagg gtacgcatgt gaggctatta
     4561 cagcaatcac aaattttgtg aattatatcg atcaagaaac agtaaaagtt atcaggacaa
     4621 ataagtgtaa ccaacgttca ataaatttag cagaaaggct taaattcaag cgagacgata
     4681 ctatggacga cattatcaat caaggagata ttgtgtttta taaataaaat actatgacat
     4741 tatctaaaaa ataaaattaa aatcccaaca gtttcttaaa aagttatgag ccacataata
     4801 aaaatgttat aattgtgcat aataaaacat agggagaatg tttaatgcat aaaaaatatt
     4861 tattgctatt ttttctgtta ttatcagttg cattgttata tagctgttcc aagcaaaatg
     4921 agaaagctca aaataaatct gttaatccaa atgagtatgc aaagcataaa tcgaaaatgg
     4981 atgaacattg ggaaacatat cacggccaag tttatcatgt gttttatcac ccattaatta
     5041 cagacccaaa agttgcgttt acaggagata cacaccaagc taagggcaac aatgattgga
     5101 tgattactgt aagtgaattt aaaaaatcat tgaacgaatt atataaaaat aattacatca
     5161 tcgttaatcc acatgatgta tacgacttat catctcatca tgtaaaagcc aaaaaaataa
     5221 aattgccgaa aggtaaaaag ccactaatca tctcaattga tgatatgaat tactatagtt
     5281 acatgagaca ccatggttat gctgaccgtt tagtactcga taaaaataaa catgttgttt
     5341 ctgaaactac agacaaaaaa ggccatgcta cttattctga tgacaatgat atcgtgccaa
     5401 ttttaaataa atttgtaaaa gagcatccag atttttcttt aaatggtgaa aaaggtgttg
     5461 ttgctttaac cggttatgag ggcgtattag gttatcgcac gaatgaatta acgagtaaag
     5521 attatacaaa gaataaaaag gcagctgagg aagttgttcg cgcaatgaaa cgggatggat
     5581 ggtcatttgc gagtcattca tatggtcaca ttaattttga aaaaacatct ttagaaggta
     5641 ttaaacgtga tactaaaaga tggaaagatg aagttgaacc aattgtaggt aaaacagata
     5701 tgtttgtatt tccacatggc gcacaggata gacatacaca agcgtatgat tatttggtag
     5761 atgaagcaga gtttaagttt attgcaggtg tcggtccaaa caattttaca gatatatcag
     5821 caacgaacgt ttaccaagat agggttgcga ttgatggtct aaacttattt gaatttaaat
     5881 ataaactcaa accgtttttt aatcctgaaa atgtatatag taaacaagat agacgttact
     5941 ttaaagggaa tcgggattat gaagaatagt taaatttaat aaaaagatag aaagaaaata
     6001 actttacata ataaggcaag gacaatatgt gcaagaagta caacttcaat ataaaaatct
     6061 tggttaagca tttttatagg cacaaatgtc ttgccttttt tattttaaaa aattttaaaa
     6121 tcaatacacc gaattttctg aaaaaggttg taatgtattg ctaaaaatga taagttatta
     6181 atcatattac actttagcgt ggtagagaag taaagcaaac aacgaaaata ccaagtgatt
     6241 ttaaatacag tattgaaagt aaagggtggt cgagtgaata attcagaaca attaattgcg
     6301 ttaaaagaat cggaaacagc atttttaaaa tattttaata aagcggatta tgagttagtt
     6361 gattttagtg tggttgaaaa gttggactgg aaacaattga accatgagga tttgcagcag
     6421 atgggtgagc gaaatttttg gcaacatgag catcaaattt atgcacttcg aaatgatttt
     6481 acagatcaat tattgcgtta ttactcaatg tatccaactg cagcaacaaa agtggcatac
     6541 acaggtttaa tcattcggaa taatgaagcg gctgtacaag ttggtttgga aaactatgca
     6601 ccatcattag cgaatgtgca acagagctta aagctcttta ttcagtttat tcaacagcag
     6661 ttgagagaca acgtgcattt tgttgtattg ggacattatc aattgcttga tgccttactc
     6721 gataaatcgt tacaaacacc agacatacta tcaatgattg aggaacgtaa tttatcggga
     6781 ctggttacat atttgtcgac tgaacatcca atcgtgcaaa tcttgaaaga gaatacgcaa
     6841 caacaattaa atgtattgga acactatatt ccaaatgacc atccagcact cgttgaatta
     6901 aaaatatggg aacgttggtt acataaacaa ggttacaaag acatccattt agatattact
     6961 gcgcagccac ctagatccta ttacacaggt ttatttattc aatgtcattt tgctgaaaat
     7021 gaatctcgag ttttaacagg tggttattac aaaggaagca tcgaagggtt tggattagga
     7081 ttaacacttt aagtaaggga gtatgcacaa tgttaagaat cgccatagcc aaaggacgtc
     7141 taatggatag tttaattaac tatttagatg taattgaata tacgacatta tcagaaacat
     7201 taaaaaatag agaacgccaa ttattattaa gtgtagataa tattgaatgc attttagtaa
     7261 aaggaagtga cgtgccaatc tatgtggaac aaggaatggc agacataggc attgttggta
     7321 gcgacatatt agatgagcgc caatataatg ttaataattt gttgaatatg ccttttggag
     7381 catgtcattt tgcggttgca gcgaaacctg aaacgaccaa ttatcgtaaa atcgcaacga
     7441 gttatgttca tactgctgaa acatatttta aatcaaaagg tattgatgtc gaattgatta
     7501 aattgaatgg ctctgttgaa ttggccggtg ttgtagatat ggtagacgga attgtcgaca
     7561 tcgttcaaac aggtactacg ctaaaagcga acggactggt tgaaaagcaa catattagtg
     7621 atatcaatgc aagattaata actaataaag cagcttattt taaaaaatca caattaatag
     7681 agcaatttat tcgctctttg gaggtgtcta ttgccaatgc ttaatgcaca acaattttta
     7741 aatcaatttt cattagaagc accattagat gagtcattgt atccaattat tcgcgatatt
     7801 tgtcaggaag ttaaagttca tggagataag gctttgaaaa tgtataatct aacattcgat
     7861 catacgaaaa cagatcattt agaaattagt catgaacaaa ttaaagcagc atttgacaca
     7921 ttagatgaaa aaacaaaaca agcattacaa caaagttatg aaagaattaa agcatatcaa
     7981 gaaagtatta aacagacgaa tcaacagtta gaagaatcag tggagtgtta tgaaatatac
     8041 catccactag aaagtgtcgg tatttatgtg cctggtggta aagcaagtta tccttcaacg
     8101 gtattaatga cagcgacgtt agcacaagta gcaggtgtgg aaaatattgt cgttgtgaca
     8161 ccacctcaac ctaatggtgt gtctcaagaa gtattagcag catgctatat tacgcaagtt
     8221 gatcaagtat ttcaagttgg tggtgctcaa agtattgctg cgttaactta tggcacagaa
     8281 acgataccta aagttgataa gattgtaggt ccaggcaatc aatttgttgc atatgccaag
     8341 aaatatttat ttggacaagt aggtattgac caaatagctg gaccaacaga aatagcactt
     8401 attattgatg aaacagcaga tttagatgcc atcgtatatg atgtttttgc acaagcagaa
     8461 catgatgaat tagcacgtac atatgccatt agtgaagatg cgcaagtcct taaagattta
     8521 gaatcacgta ttgctaaagc attgcctaat gtggacagat acgacattgt ttctaaaagt
     8581 atcgctaatc aacactacct tatccatgct agtaattttg atgaagcatg ccatgtcatg
     8641 aatacaatcg cgcctgaaca tgcgtcgatt caaacagtaa atcctcaacc atatattgaa
     8701 aaagtgaaat atgtgggtgc attgtttatt ggacattatt cgccagaggt cataggagat
     8761 tacgttgcag gtccaagtca tgtattacct acaaatagaa cagctagatt taccaatggg
     8821 ttatcggtca atgatttctt aacacggaac acggtcatcc atttatcaaa agatacgttt
     8881 gaacaaattg ctgattcagc acaacatatt gctcatgttg aagcattata caatcaccag
     8941 cagtctattt taatacgtca gtcttagggg agtgtaattg aaatgattta tattgataaa
     9001 aatgaaagtc cagttacgcc gttggatgaa aaaacaatga cgtctattat tagtgcaacg
     9061 ccatataatt tatatcctga tgcagcatat gaacaattca aggaagctta tgctaagttt
     9121 tacggattat cgcctgaaca aattattgca ggaaatggat ctgatgaatt gattcaaaag
     9181 ttaatgctga tcatgccaga aggtccggca ttaacgctaa atcctgattt ttttatgtat
     9241 caagcatatg cggcacaagt aaatcgtgaa attgcatttg tagatgcagg atcagattta
     9301 acgtttgatt tggaaaccat tttaacgaaa atcgatgaag tacaaccatc attttttatt
     9361 atgagtaatc cacataaccc ttcaggcaag caatttgata cggcattttt aacagctatt
     9421 gcagataaga tgaaagcatt aaacggatac tttgtcattg atgaagcata tttagattat
     9481 ggtacggcat atgacgtgga actggcacca cacatcttaa gaatgcgtac attatcaaag
     9541 gcgtttggaa ttgccggctt aagattaggt gtcttaatta gtactgctgg aacgataaag
     9601 catattcaaa aaatagaaca tccatatcca ttaaatgtat ttacgctaaa tattgcgact
     9661 tatattttta gacatagaga agagacaaga caatttttaa cgatgcaacg acagttagct
     9721 gagcagttaa aacaaatatt tgatacacat gttgcagata aaatgtcagt gttcccatca
     9781 aatgctaatt ttgtacttac taaaggctca gcagcgcaac aattaggaca atacgtatat
     9841 gaacaaggat ttaaacctcg cttttatgat gagccggtga tgaagggcta tgtaagatac
     9901 tcaattgcaa cagcatcaca gttaaagcaa ttagaagaaa ttgttaaaga atggagtgca
     9961 aaatatgatt tatcaaaaac aacgaaacac agctgaaacg caactaaata tttcaatatc
    10021 tgatgatcag tcaccatcgc atattaatac aggtgtgggc tttttaaatc atatgttaac
    10081 cttgtttaca tttcatagcg gtctgtcatt aaacattgag gcacaaggtg atattgacgt
    10141 agatgatcac cacgtaactg aagatatcgg cattgtcatt ggccaattgt tacttgaaat
    10201 gattaaagat aaaaagcatt tcgttcgtta tggaacgatg tacattccaa tggatgaaac
    10261 attagcacgt gtcgttgtgg atataagtgg gcgcccatac ctatcattca atgcatcatt
    10321 aagtaaagaa aaagttggta cgtttgatac ggagttagta gaagaatttt ttagagcggt
    10381 cgtaatcaat gcaagattaa caacgcatat tgatttaatt cgtggaggta atacacacca
    10441 tgaaattgaa gctatattca aagcgttttc ccgtgcatta ggcatagcgc taactgcaac
    10501 tgatgatcag cgtgtgccgt catcgaaagg tgtgattgaa tgattgtcat cgttgattat
    10561 ggattaggga atattagtaa tgtaaaacgc gctattgaac atttagggta tgaggtggtt
    10621 gtctcaaata cctcaaaaat aatcgatcaa gcagaaacaa tcatattgcc cggtgtcggc
    10681 cattttaaag atgcgatgtc agagataaaa cgattaaatc tcaatgcaat attggctaag
    10741 aatactgata agaagatgat tggtatttgt ttaggcatgc aattaatgta tgagcatagt
    10801 gatgaaggcg atgcatctgg attagggttt atcccaggaa atatttcgcg tatccaaaca
    10861 gaatacccag tgccacactt aggctggaat aatttagtga gtaagcaccc tatgttaaat
    10921 caagatgttt atttcgtaca ttcttatcaa gcgccgatgt cagaaaatgt aattgcatat
    10981 gcgcagtatg gggctgatat tccggcaatt gttcaattta acaattatat tggtattcaa
    11041 ttccatcctg aaaaaagcgg tacatatggg ttacaaattt tgcgtcaggc aatacaaggg
    11101 ggatttataa atgattgaat tatggccagc gattgatttg attgggtcaa caagtgtgag
    11161 gttaacagag ggtaaatatg atagtgaaga aaaaatgtca cgctcggctg aagaaagtat
    11221 tgcttactat agtcaatttg aatgtgtgaa tcgtattcat atcgtcgact tgataggtgc
    11281 taaggcacag catgcccgag agtttgatta tattaagtca ttgaggagat taacaaccaa
    11341 agatattgaa gtaggtggtg gcattcgtac gaagtcacaa atcatggact actttgccgc
    11401 agggattaat tattgcatag ttggaacgaa aggtattcaa gatactgatt ggcttaaaga
    11461 gatggcacat acatttccag gtcgcattta tttatctgtt gatgcctatg gagaagatat
    11521 taaagtgaac ggatgggaag aggacacaga gttaaattta tttagttttg tgagacggtt
    11581 atcggatata cctcttggcg gcattatata tactgatatt gctaaagatg gcaaaatgtc
    11641 cggacctaac tttgaattaa ctggtcaatt agtaaaggca acgacgattc ctgtcattgc
    11701 ttccggtggt attagacatc agcaagatat tcaacgatta gcgtcgctaa atgttcacgc
    11761 tgctattata ggaaaggctg cacatcaagc atctttttgg gaggggctaa aatgattaaa
    11821 aaacgtatca ttccatgttt agatgtcaaa gatggtcgtg tcgttaaagg gattcaattt
    11881 aaaggattaa gggatattgg gaatcctgtt gatttagcaa tgtattacaa tgaagcgggt
    11941 gctgatgaat tagtattttt agacatctct aagacggaag agggtcatag cttaatgcta
    12001 gaagtgattg aacagacagc gtcacgcttg tttatccctc ttactgtagg gggtgggatt
    12061 caaagtctcg atgatattac ccaattgcta aatcatggtg cagataaagt atcattaaat
    12121 tcaagtgctt taaaaaatcc acagctcatt aaacaagcga gtgataaatt cggtagacaa
    12181 tgcatctgca tagcaattga tagctattat gatcctgaaa gaaaagcaca ttattgttgt
    12241 acgactggtg gtaaaaaaat gacaaatatt aaagtatatg actgggtaca gcaagtagaa
    12301 cagttaggtg caggtgagct cctcgttaca agtatgggac atgatggtat gaaacaaggc
    12361 tttgatattg aacacctagc aaatattaag tctcttgtaa atattccaat cattgcttct
    12421 ggtggtggtg gcaatgcaca acactttgta gaattatttg atcagacgga tgtttctgca
    12481 ggtttagctg caagtatatt acatgatcga gaaacgacgg ttcaatctat taaagaagtg
    12541 atacggcaag ggggtatagc agtaagatga ccaaatataa aattgatttt agcaaaggtt
    12601 tagtgccagc aattttacaa gataatcaaa caaaacaagt attgatgttg ggttatatga
    12661 accaagaagc ttttgataaa acgatagaag atggtgtggt atgtttctat tcgcggtcga
    12721 aacaacggct atggacaaaa ggtgaaacat ctggtcatac gcaacgtgtt aaagatattc
    12781 atgtagattg cgacaatgac actattttaa ttgatgtcat accaaatgga ccaacatgtc
    12841 atacaggcag tcaaagttgt ttcaacacag aagttccatt ttcagtgcaa acattagcgc
    12901 agacagttca agatagtgcc caatccaata atgaaaagtc atatacaaaa tatttattaa
    12961 cagaaggtat agaaaagatt acaaaaaaat acggtgaaga agcttttgaa gtcgtaattg
    13021 aagcaattaa aggtgacaaa aaagcatttg taagtgaagt agcagatgaa ctttatcatt
    13081 tatttgtctt gatgcatgcg cttggcgtcg atttttcaga aattgaggcg gaattagcgc
    13141 gtagacatca taagcgcaat aactttaaag gtgaacgaca aaatatcgaa cagtggtaaa
    13201 gcaagtatgg actaagatat aaggaaaagg atcatggctt atacacttac aaatattgtg
    13261 gaaaacgtga cattttcaag tttaaaatac gacaccaaca tattttaact atgaatgctg
    13321 tgatggtact aaagttgcga actcgttata gataagtagt ggataatcac aatacgaaat
    13381 caaaaataat tataaaaagt aaattgagca actcaggaat agatgtcact gttaaagatg
    13441 tcgaaaagta tatgaatcga tataatgaag ttatgaaggg aaaaaatggc gaaaaagcta
    13501 aagagttatg tttgtcgtta ctacctatta atatcatagt tgtctttaca ttctttgtat
    13561 ttatactata aatacaaata tatctagcct gaaatagaaa tgtcatagcc tatttaaaag
    13621 acaatctcca ttagaactaa gatatgcatc ccgaaagtta gactaaaaaa ctaactttat
    13681 gggatgtatt tttatgctaa tcatcataaa ttcgagatta agttaaaggt agttcaagag
    13741 taattaaaca ataaattaaa aatagtagga tacttacttt gagggaagaa aattaactgt
    13801 atatatttag tttaggaaca agtattacgg tttatcctga tacaattatt gtggatggga
    13861 tgatattttt aggtttaaaa tacgacacca gcaaacataa taactgtaat agctcataaa
    13921 tctccccata tagctaatct aaaaaaataa tacatcattg gaattaagcc ccaagcatgt
    13981 aaatattaaa aatcaaaaaa gatatatgta aaaaagtta
//
LOCUS       DAKTLH010000066        13831 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.066, whole genome shotgun sequence.
ACCESSION   DAKTLH010000066 DAKTLH010000000
VERSION     DAKTLH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13831
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.066"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            38..997
                     /locus_tag="O0394_001582"
     CDS             38..997
                     /locus_tag="O0394_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0499662.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK"
     gene            complement(1043..1174)
                     /locus_tag="O0394_001583"
     CDS             complement(1043..1174)
                     /locus_tag="O0394_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499663.1"
                     /translation="MRYVTMRQFIKRTVKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
     gene            complement(1238..1453)
                     /locus_tag="O0394_001584"
     CDS             complement(1238..1453)
                     /locus_tag="O0394_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCY0499664.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
     gene            1618..2169
                     /locus_tag="O0394_001585"
     CDS             1618..2169
                     /locus_tag="O0394_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0499665.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVENKVAGCIISYSGENELKYEKAWELLDLPEKIKQYGTPLP
                     VKEAKDDEYYIETIATFAAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(2221..3159)
                     /locus_tag="O0394_001586"
     CDS             complement(2221..3159)
                     /locus_tag="O0394_001586"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCY0499666.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
     gene            3341..4702
                     /locus_tag="O0394_001587"
     CDS             3341..4702
                     /locus_tag="O0394_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCY0499667.1"
                     /translation="MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
                     MDLFNNISDIDVDEQIGGITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            4689..6362
                     /gene="menD"
                     /locus_tag="O0394_001588"
     CDS             4689..6362
                     /gene="menD"
                     /locus_tag="O0394_001588"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCY0499668.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTVNRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            6349..7152
                     /gene="menH"
                     /locus_tag="O0394_001589"
     CDS             6349..7152
                     /gene="menH"
                     /locus_tag="O0394_001589"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCY0499669.1"
                     /translation="MTHYKFYEANVETNQVLVFLHGFLSDSRTYHNHIEKFTDNYHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPHKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            7145..7966
                     /gene="menB"
                     /locus_tag="O0394_001590"
     CDS             7145..7966
                     /gene="menB"
                     /locus_tag="O0394_001590"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCY0499670.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(8205..8534)
                     /gene="sspC"
                     /locus_tag="O0394_001591"
     CDS             complement(8205..8534)
                     /gene="sspC"
                     /locus_tag="O0394_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HCY0499671.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(8572..9753)
                     /gene="sspB"
                     /locus_tag="O0394_001592"
     CDS             complement(8572..9753)
                     /gene="sspB"
                     /locus_tag="O0394_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HCY0499672.1"
                     /translation="MNSSCKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSAKLKQELKNTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCATFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            complement(9835..10845)
                     /gene="sspA"
                     /locus_tag="O0394_001593"
     CDS             complement(9835..10845)
                     /gene="sspA"
                     /locus_tag="O0394_001593"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HCY0499673.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQS
                     SKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPD
                     AA"
     gene            complement(11373..12527)
                     /locus_tag="O0394_001594"
     CDS             complement(11373..12527)
                     /locus_tag="O0394_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0499674.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPLFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
     gene            complement(12723..13733)
                     /locus_tag="O0394_001595"
     CDS             complement(12723..13733)
                     /locus_tag="O0394_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCY0499675.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIVYVIHPINADWLLGSSP
                     YTSLENEGQSIFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRPSQFK
                     DS"
BASE COUNT         4840 a         1966 c         2448 g         4577 t
ORIGIN      
        1 atatatattt ctactaaagt tgtttggagg taaatgtgtg aataggaata tcgttaaact
       61 agttgtgttc atgctaattt tagttgtagc agtagcgggt tgtggtcaaa aagatactga
      121 agagaaaact gaaatgacga caataaaaga tgaattagga actgaaaaaa ttaagaaaaa
      181 tcctaaacgt gttgttgtat tagaatatag ttttgctgat tatttagcag cattagatat
      241 gaaacctgtt ggtattgcag atgatggcag cactaaaaat ataacaaagt cagtaagaga
      301 taagattggg gcatatgaat cggttggatc tagaccgcaa ccgaatatgg aagtgataag
      361 taaattaaaa ccggatttga tcattgcaga tgttagcaga cataagaaaa tcaaatcaga
      421 attgagcaaa attgctccga caatcatgtt agttagcggt acgggagatt ataatgcaaa
      481 tattgaagca tttaaaacag tcgctaaagc agtaggcaaa gagaaagaag gcgagaagcg
      541 tctggaaaag catgataaaa tattagcgga gattagaaag aaaattgaac agagtacgtt
      601 aaaatctgca tttgcattcg gtatctcaag agcaggtatg tttattaata atgaagatac
      661 atttatggga caattcttaa ttaaaatggg tattcaacct gaagtcacaa aagacaaaac
      721 tacgcatgtt ggtgaacgca agggtggtcc ttatatatat ttaaataatg aagaacttgc
      781 caatatcaat ccaaaagtta tgattttagc cactgacgga aaaacggaca aaaatagaac
      841 gaaattcatt gatcctgcag tttggaaatc attaaaagct gtgaaagata acaaagttta
      901 tgacgttgac cgaaataagt ggttgaaatc aagggggatt atcgcaagtg aaagtatggc
      961 agaagattta gaaaaaattg cagaaaaagc aaaataaaaa tacagcgcta ctcgtaaatc
     1021 atataagagt ggcgctgtat ttttaattat gtttattatc tgtcggatgt gatgatttac
     1081 ctgaaagttt atttcgaata aatttaatta cataaccgac aaggattgtt ttaacagttc
     1141 ttttaatgaa ttggcgcatc gttacatacc tcatttctct atatcttacg aactatatac
     1201 ccattcatat atgcttttta aacgtcattg tcacaattta atttttaggg aatataatat
     1261 aaccatcttt atctgctttt ttagtaaaaa tgacaaaaat tgcatgtatt attgagatga
     1321 tggtagggat acctgtccag aaaaataata agtgaaaaag accttgtcca aatttatcag
     1381 cataaaattt atgaatacct aaacctccaa gaaataatgc aacaataaca taaatggctt
     1441 tattgacttt catttgtaaa tcctccttaa ctataattct acttaaattc gttgtgaaaa
     1501 ccaatatttc taactttaga attttcaaac tttctaaaat tataagtata tctttttaaa
     1561 ataagctaga atttctatat aataaatgtt aataacgtaa aagggaatga tgacatagtg
     1621 atacgtcaag cacgtccaga ggaccgattt gatattgcga agttagttta tatggtttgg
     1681 gatgatatgg aattagaatt ggtaaagcat ctacctaaag acatggtatt agatgcaatt
     1741 gaaaaaagct gtgttgatgc aacatatcga actttttatc agcatatttt agtttatgaa
     1801 gtagaaaata aagtagcagg ttgtattatt agctatagtg gtgaaaatga attgaaatac
     1861 gaaaaagcat gggaactact tgacttgcca gaaaaaataa aacaatatgg cacgccatta
     1921 cctgtaaaag aagctaaaga cgatgagtat tatatagaaa caattgcgac atttgcagca
     1981 tatagaggta gaggcatcgc gacaaagtta ttaacgtcat tacttgaatc aaatacacat
     2041 gttaaatgga gtttgaattg cgatattaat aatgaagcag cattaaagtt atataaaaaa
     2101 gtaggcttta tatctgatgg acagattgaa ttatacaagc acatgtatca tcatttaatt
     2161 gttaaataaa aaaactcgac agttcgatgt aagtcgattg ccgagtagta tcattttcta
     2221 ttaaatgcct gcaaataatg cactaatata aatacctaat gcatataata aaccgaaaaa
     2281 tgtatttgtt ttaccagcag cagccattgc tggcatcatt gtaggcggtg tatcattctt
     2341 cttgaaacgt ctgataactt taacaggcat tgggaatgat aacaacgcaa gtaagtaaaa
     2401 taatgagcca ccaggtttaa taatgatcgt aagtacaata aaggcataag cgataaagta
     2461 catgattgcc ataaatgtta aagaagcatt tttacctaat agaatgggta aagttttgcg
     2521 accacttgct ttatctttga cacggtcgcg aatattgtta gccatattaa ttaaaccgat
     2581 agtgattact ataggtacac ttaaccaaat tacataactt tgaatattgc cagtttgaat
     2641 aaagaatgca ataacgataa taaacatacc cataaatacg cctgagaata attcaccgaa
     2701 aggcgtccat gaaataggga aagggccacc tgtatatagg taaccaacag ccatacatac
     2761 taatccaact ggtaataacc aaaatgaaga gttagcagct aaaaacaaac ctaatattgc
     2821 tgctaagatg taaaatgcaa tggctaatcg tagcacaagc tctgggctca taccgttgcg
     2881 aacaatggca ccaccaatgc ctacagattc atgatcatca aggccttttt tataatcata
     2941 gtattcatta aacatattag ttgctgcttg aataagtaag catgctagta acatggcaat
     3001 gaataggctg attttaatat gatcttcgct accaagaaaa tatattttag atgctgctgt
     3061 accaactaaa acgggtacta cggaagcagt taatgtatga ggacgcatta aatgccaata
     3121 tttcttaact gtagaatatt gctgatattg attactcatt gaatacaaaa cctctttaaa
     3181 tcatattttt tataacaaag atattttaat agaaagtagc ataaaggtca attgacgtga
     3241 aaaattgcaa gataagttta agcaattctt gtaacacaat tatccaaaaa tgatacaata
     3301 taataatgag tgaaaaaaga cgaagaaaga agtgaaatgg atggctacgg gcgtattaga
     3361 ggacgatatt gtcaaagaga tatatggcag ctcaaaggaa tgggtttcag ttgaagtgaa
     3421 attatcacag tcactagacc cgagcacatt atttcatctc actgacaatg aggcaggaga
     3481 tcgcttttat atgcgtttga atgataatcg aacgtcatat tttggctaca aagcaattca
     3541 attattcaaa aataattcta aaaataaaca atctattttt aaagactggg aaaaattaaa
     3601 acataacatc acatttatac atccgcaatc tgagaaacat catcttcgag ttgttggagg
     3661 gttccaattt tcaagtcata aatcagatga tgaatggcga gagtttggac taaatcattt
     3721 tgtattacct gaagttttaa tttcaactga taataatggg acatttttaa cttatacagt
     3781 taaaagggaa agttttactg ttgaggcatt gaacgattta atggatttgt tcaacaatat
     3841 atcggacata gatgtggacg agcaaattgg gggaattact agaaatgaag atatttataa
     3901 agatgactgg cgtcaacttg tagtagaagc tatagaatct attaataatg aagaaaaaat
     3961 tgtactagca cgtagacggt taataaagtt cgataaagat atcagtattc catatattct
     4021 aaagcaagca tattctaaag aaaaaaacag ttatatattc ttgttagaat cacaagattc
     4081 tatattcttt tcacaaacac ctgaacaatt aataaaggtc aataataaaa tactatcgac
     4141 taaagctgta gcaggtacaa ttaaacgttc acaagatgag gacgaagata caaaaaatgt
     4201 tgaagcattt ttaaaagata ataaaaactt aatcgaacat cgatttgttg ttgacagtat
     4261 tttacatgat attaaacctt atatcactga attacattat gataagacgc ctaaaattct
     4321 aaaaaatgat catttatatc acttgtacac tgaaataaag gcgccactga aggatgattc
     4381 gtatattagt ttaattgata atttacatcc aacacctgct ttaggtggct atccaaaaga
     4441 atttgcaatg gattttattg agcagaaaga atttggtacg cgaggattat atggtgcacc
     4501 ggttggctat atagatatat acgatgattg tgaatttatt gttgcaattc gttcgatgct
     4561 tattaagaaa gcacaagcaa ctttatttgc tgggtgtggc attgttaaag attctgatcc
     4621 agatagtgaa ttggcagaaa cgaaccttaa gttcacacct atgatgaatg cattaggagt
     4681 cgatatgaat gggaaatcat aaagcagctt taacgaagca agtttttaca tttgcatctg
     4741 agttatatgc gtacggcgta agggaagtag ttatcagtcc gggatcacgc tcaacgccac
     4801 ttgcacttgc atttgaagca catccaaata ttaaaacatg gatacacccc gatgagcgaa
     4861 gtgcagcgtt ttttgcagtt gggttaatta aaggcagtga aagacctgtc gctatattat
     4921 gtacgtcagg tacagcagca gcgaattata cgcctgcaat tgctgaaagc caaattagta
     4981 gaattccttt aatcgtttta acaagtgacc gtccgcatga attaagaagt gtaggcgcac
     5041 cacaagcgat taatcaagta aatatgttta ataattatgt aagttatgag ttcgatatgc
     5101 ctattgcgga tgatagtaaa gagaccattg atgcaattta ctatcaaatg caaattgcta
     5161 gtcaatattt atatggacca cataaagggc caattcattt taacttgcca tttagagatc
     5221 cgttaacacc tgatttgaat gcaacagaat tgttaacttc tgagatgaag attttaccgc
     5281 actatcaaaa aagtatagat gcatcggcat taagacacat tttaaataag aaaaaaggtt
     5341 taattattgt aggggatatg cagcaccaag aagttgatca aatactaacg tattcaacga
     5401 tatatgattt gcctatttta gctgatcctt taagtcattt aagaaaattt gatcatccga
     5461 atgttatctg tacatatgat ttgctgttta gaagcggctt agacttaaat gtggatttcg
     5521 taattcgtgt tgggaaacca gtgatttcta aaaagttaaa tcaatggtta aagaaaactg
     5581 atgcatttca aatattagtg caaaacaatg ataagattga tgtctttccg atagcgccag
     5641 atatttcata tgagatttct gcgaatgatt tctttaggtc attaatggaa gacacgacag
     5701 ttaatcgcgt aagttggtta gaaaaatggc aatgcttaga gaaaaaaggg cgtaaagaaa
     5761 ttaaatgtta tttggaacaa gctacagatg agagtgcatt cgttggtgaa ttgattaaga
     5821 aaacatctga aaaagatgca ttatttatta gtaatagtat gcctatcaga gatgtagata
     5881 acttgttatt gaataaaaat atagatgtct atgcgaatcg tggtgcgaat ggtattgatg
     5941 gtatcgtttc aactgcactg ggtatggctg tgcataaacg aataacatta ttgataggtg
     6001 atttatcatt ttatcatgat atgaatggac tattaatgtc aaaattaaat aatattcaga
     6061 tgaatattgt attattgaac aacgatggtg gcggtatttt ttcatattta ccacaaaaag
     6121 aaagtgcaac tgactatttt gaacggttgt ttggcacacc gacgggattg gatttcgagt
     6181 atacagctaa gttatatcaa ttcgatttta aacgttttaa cagtgtttca gaatttaaaa
     6241 atgccacatt gttatctgaa acttcgacga tttatgaatt gataacgaat cgcgaagata
     6301 actttaaaca gcatcaaatt ttataccaga aattgagtga aatgattcat gacacattat
     6361 aaattttatg aagcaaacgt tgagaccaat caagttttag tatttctgca tggatttctt
     6421 agcgacagcc gtacttatca taatcacatc gaaaaattta ctgataacta tcatgtcatc
     6481 actatagact taccaggcca tggcgaagat cagtcttcaa tggatgaaac gtggaatttt
     6541 gattatatta cgacgttgtt agaccgaatt ttagataaat ataaagataa atcaataaca
     6601 ttgtttggat attcaatggg tgggcgtgtt gcattatatt atgcaattaa tggtcacatc
     6661 cctatatcta atttgatatt agaaagtacg tcaccaggta ttaaagaaga agcaaatcaa
     6721 ttggaacgcc gtcttgttga tgatgcacgt gctaaagtat tagacatagc aggtattgaa
     6781 ttatttgtta atgattggga aaagttgcca ttatttcaat cgcaactaga attaccagtt
     6841 gaaatacaac atcaaataag acaacaacga ttgtctcaat cgccacataa aatggccaaa
     6901 gcattaagag attatggtac aggtcaaatg ccaaacttat ggccgcgcct gaaagaaatt
     6961 aaagtaccaa cattaatatt agctggagaa tatgatgaaa aatttgtaca gattgcgaaa
     7021 aaaatggcaa atttaattcc taatagtaaa tgtaaattaa tttctgctac aggtcataca
     7081 attcatgtgg aagatagtga tgaatttgat acaatgatat taggattttt aaaggaggag
     7141 caaaatgact aacagacaat gggaaacact tagagaatat gatgaaatca aatatgaatt
     7201 ttacgaaggg attgctaagg taacaataaa tcgccctgaa gtacgcaatg cgtttacacc
     7261 taaaacagtt gctgaaatga ttgacgcatt ttcacgtgca cgtgatgatc aaaacgtttc
     7321 agttatcgta ttaactggtg aaggtgattt agcattctgt tctggtggtg accagaagaa
     7381 acgtggacat ggtggttatg taggtgaaga ccaaatccct cgcttaaatg tattagattt
     7441 acagcgttta attcgtatta ttccaaaacc ggttatcgcg atggtaaaag gttatgctgt
     7501 aggtggcggt aatgtactaa atgttgtttg tgacttaacg attgctgctg ataatgctat
     7561 ttttggacaa actggtccta aagtaggttc atttgatgcg ggttatggtt caggatattt
     7621 agcacgtatc gttggacata agaaagcacg tgaaatttgg tacttatgtc gtcaatacaa
     7681 tgcacaagaa gctttagata tgggtctagt aaatacagtg gtacctttag agaaagttga
     7741 agatgaaact gtgcaatggt gtaaagagat tatgaaacac tcaccaacag cgttacgatt
     7801 ccttaaagca gctatgaatg ctgacacaga tggtttagct ggtttacaac aaatggctgg
     7861 ggatgcaaca ttgctttatt acacaactga tgaagcgaaa gaaggccgtg atgcgtttaa
     7921 agaaaaacgt gatcctgact tcgatcaatt ccctaaattc ccataagtta ttttgaatat
     7981 gatattaagt cacttgcgtc gtttattagc gacgataggt ggctttttta ttttataaga
     8041 atttaaaagg gcattttaat taattatcat aaagataaga tagtttgaga aaaattaata
     8101 gagaaagaat aaatgttaaa gattattatt acaatgaaaa aacgagctgg tgagaaattg
     8161 aattctcgcc aacccgcttt atgaagtgaa atcatttaaa ttagttatac taagcgctca
     8221 taaacgattg gtcgcgaagt gccaatacct tgtttggatg acgtttgcat gaggatatgc
     8281 tgtgtatcat cataatcaag aatataaatg atttcttcat tatcaataga tgaaaattta
     8341 atgcgttgat gtgccgtatc tataaaaaga atatgatatt gattgtgact tgtatcatcg
     8401 ccatgacgta tacaaattga tttttgtaat tgatgattac tccaattacc aatgaataaa
     8461 ttgatattgg tttgttctag atgtgtgagt ttggttgtgt cgtaaactaa atttataaat
     8521 tgtagttgat acatagtttt acctgccttt aatcaatatc tatattactt tttagtaacc
     8581 tatcattgaa ccataccagt tataatcacg attgaatgat aaatgtaata ggcttgaatc
     8641 tgcatcttgg attgataatt ctgtatccca aggattccag taaataagtt tttcttggtc
     8701 attaatttta gcattaccaa caactgctag cgcatgtcct aaatgtgggt cattagggtt
     8761 ttgagataca ctttgtgcaa ggatcataat tcctacatta tcttttgtaa gttgatcaac
     8821 ttgttcatat gatggtacgc cttcttgata atgaatatct ctgccttgtg atttaccgta
     8881 ttcaatcatt tgattaggga atgttgcaca attaggaagg tcttgctcac ttacttcagg
     8941 gtataatgta cgcataatat catgtgcatt ataagtgtct gtatttttag ttgcatttaa
     9001 taatgctgcc atactgaatc ctgcacacca tgagttatcg aattgttgtt ctctaatttt
     9061 gaagtttttt aatgtatttt catattgaac ttgatcttct tgtatcgctt cgttttcttc
     9121 aactttagta ggtgttactg tattttttaa ctcttgtttc aattttgctg aaactgtttt
     9181 agcactttct ttttctttta cattaccagg tagaggcgta gcttttacta atctaacttt
     9241 gccatcttct tcaaaataaa accctttttc atcagtaaga acagtgatat ttgaattttt
     9301 atctttaatt tggtctaaat ctttagcgat gaagtttgaa attttaacac tgtaattcat
     9361 gtcttctttt gatttgttta aatcatcttt atttttagga ctaagtgtta aagtataaac
     9421 aattttaccg tcttttataa ctggataata gtaactatta tcttcttcac cattaaattt
     9481 ataaatttta aaagcttcgc caagttcata tttaccagtt tttgcattac tttgtttatc
     9541 taatgcttta gcataaccag caaattgttg ttgtgctaga tcttttactt tttgaggtac
     9601 tttgtcactc ttaacattaa tttctagctg tttagagtgt gaatcggctt tggctttatt
     9661 gttattagca aatgcgccta gtgataaaat aagcattgaa accattatga tgcttataat
     9721 attgaatact ctagatttac atgaactatt catataatac cctccattta aatcaaaata
     9781 ttttacgttg ctaagacttt gcttctattt tatctagacg aacttaattg ttaattaagc
     9841 tgcatctgga ttgtctgaat tattgttatc gccattgtct ggattatcag ggttgttagg
     9901 gttgtcaggg ttatttggtt catctgggtt gttagggtta tcaggattgt tagggttatc
     9961 aggattatct gggttattag gttggtcatc gttggcaaaa tggatatctt caatattttg
    10021 ttttaagaag ttgcgtacat tttcattaat aaataccgca ccattaaatt catttggtac
    10081 accgccccaa tgaattccga tcacttcatt tttttcatta aatacaggtg aacctgaatt
    10141 accaccagtt gtacttaaat catattgcat agcttcgcct ttgaggtaag tgatttttcc
    10201 tttactttcc cacattgttg ctacaggttt atcaccagga tatcctgtta cagtaatatt
    10261 ttggttaact tgtgtttcag cattattact cattgttgct ggtttaacta cttcaccaat
    10321 atgtttgttt tgctcattag gggagaattt aactattgct aaatcacctt cgcctgaata
    10381 tttagtgatt tgttcagcag tgaaaccacc atttggataa ttgtcttggt taattgcaga
    10441 agggaatgct tttaaagcat gaggatcacc gtgcgtagca tctacgacgt gtttatttgt
    10501 taaaagagta tctttaccta caactacacc ggaagcaata aatgtaccag taggtgcttc
    10561 aacttgaata taagttacgg gtgcataatg accattcgtt gtatctgtga tttggtgacg
    10621 atcgttattt ggtaatataa catttgcgtg ttcacgttgt tctaatggtt taaggttacc
    10681 gcctttttga atcttaggtg tttgctgttt gcttgactgc gtttgttgtg gatgattgtc
    10741 catagccttt gaagataacg cgtttgctgc tggagaactc acaagtgtcg ctgttgtcaa
    10801 agttgcaacg aataaagaac taacttttaa aaatttacct ttcatctaaa aacctccaaa
    10861 aaatttattt acaagttaaa tataacacta aaaattttta agtcaataag aatatataaa
    10921 taaattaaaa agtttttgta aagattagag tttacaaaaa cattaaggga attaaaattg
    10981 aattgtatta tactttattc aattgagtta aattgataag atcgtcaatt gtttgcattt
    11041 atctaaaaga ataaataaga aaggaattga gagcgaatgg aaagtatttg tttataataa
    11101 taaatagtaa cttttgaata ataattgttt ggccaaaggt agattaatat gaatggtgat
    11161 atattatttg agtgcttaag tttcgaagaa caattataag ttagcatatt aaaacctttt
    11221 gatagaatca ttttgagtga ggtgtttaaa ggagaggaat ataactcggt agtatttaca
    11281 gtctctatag agtattattt aaaacttata gaatataaaa aactgaacgt actatgaatt
    11341 tagttcacga cgtccagttc aacatatcat ttttatttag tatttaaata tttagcaagt
    11401 ctcttcatac cttcttttaa aacatccatt tcataagcat aggaaatcct tacaaatcct
    11461 ttaccgaatt ctgtaaagga tgagcctgga acgattgcta aatgtgttga ttcaagtaaa
    11521 tcgacacaaa attcgaaatc gtcatcggtg atatgtttaa tacttgggaa aatataaaac
    11581 gcgccttcag gttgagcggt aatctcaaaa cctaatttag ttaattcaga tactaaataa
    11641 tttcttcgtt ctacataagc ttcgttcata tatttaggag cttctaatcc ttcgttaagt
    11701 gctgttatac atgctatttg agctggaaca ttggcacaaa tacaattata ggcgtgcata
    11761 aatgttaatt tatcaatcaa atattgaggt cctaatagaa aaccaattct tattccggtt
    11821 gctgagtgtg atttacttaa accaccaatt aatattaatt gatcacgaat gtcttcaaat
    11881 tcagcgaagg atacatgttt accactaaat gtattttcag cataaatctc atcgctaata
    11941 ataaataacg gatatttttt taatacattt acgatattta aaacttcatt tctttttaaa
    12001 actacgccag ttggattagt cggataattt aacaagacag cttttgtctt tggagaaata
    12061 tgactttcta atgcatcagg tgtaatttta aattgtgttg ctgttgtatc aatataaatt
    12121 ggtttaccac ctagtacttc gatgagtggt atgtagcctg cataaattgg tcccggtatt
    12181 ataatttcat ctccaggctc tatgatagaa cgtaacgttg tgtctattgc ttcacttgct
    12241 ccatttgtca caataatttc ttcaggatca taggaaaaat gataacgatt tttgaagtat
    12301 tgactaattg cttcgcgagt ttctaataac cctttattgt gagagtatga tgtcttgtca
    12361 ttgttaatag catcaatata tgcctttttc acaacatcag gcatcgggaa gtctggttgg
    12421 ccaatagtta aattaacaca atcatccaaa ttattcatac gatttgaaaa ttggcgaata
    12481 cttggtgctc ttaaatattt agaattagaa tttaaagaaa gtttcatttg tgacacctca
    12541 aatataaatc aaatattgtc taaaaattta gaaattaata atatcataac atacttttaa
    12601 agcaaaaaag ggtaaattac ttaagcttta tttgaaaaac gaaaaatgtc taaatacact
    12661 gtagtaacta cttttcaaaa tgaataaagt ggttacagtt aatgtactta gacagtataa
    12721 aattatgaat ctttaaactg tgatggtctt tgtaaattaa ttattggatt tgtccattta
    12781 caaacaaagt ttgtagataa tacatatacg atgattacag atatacttat taaataaaga
    12841 taagtcatta atgatatagg attatcgaat gggtaccatt caaatcctct aacaatgcca
    12901 ataattaaac catgtaataa atatacgtat agcgtacgac taccaatata agtatataat
    12961 tttttctttg ttgacattaa atttagaaac gcagtcattg cgattaatat aattccatat
    13021 aatataagtc gtttaaaagg actgaatata ctctgtcctt cattttcaag tgaagtatat
    13081 ggtgaacttc ccaataacca atctgcattg ataggatgaa tcacgtaaac gataaaaaac
    13141 aaaataaagg taatgataga tactggtatt agttttttat ttttaaaaat agccgtatgt
    13201 tttttggtga aaatgtaacc tagataaaat attgggaaaa atacgattgt ccttgaaatg
    13261 cttaagtagc tatcgatgtt atctgaaaaa cctgctccaa tagatataat aattgaaact
    13321 gatagcactt tatatggatt aaatcttcta actattacta aaatgacatg aaagaaaaat
    13381 agcgtgatca aaaaccataa cgcaaatact gggttaaaag gatcaagttg taattcgtca
    13441 cttttacctg ttaagaaata ataaattgaa aagaatgcaa aaaatatcat ataaggtact
    13501 atcaaacgtt ttgaaatttt ttctaaatag tatggtttat caatattttt cgcgaaataa
    13561 ccagatataa acaaaaatgt tggcatatgg aaactataaa taactaggta taatgctgat
    13621 aaatatttat cgccactagt gtagggttgt aacatatgtc caaatacgac taataatatt
    13681 aaaattgctc ttgcgttgtc aaaaaaataa tctctctctt ttaatgaagt catattttat
    13741 ctcctttatg tccataagac tcttactaat ttattatatt ttgtattagt tatgcaacaa
    13801 atttgtctat tgtgtcgaat agtattgtaa a
//
LOCUS       DAKTLH010000067        13518 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.067, whole genome shotgun sequence.
ACCESSION   DAKTLH010000067 DAKTLH010000000
VERSION     DAKTLH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13518
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.067"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            36..4181
                     /gene="sdrD"
                     /locus_tag="O0394_001596"
     CDS             36..4181
                     /gene="sdrD"
                     /locus_tag="O0394_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrD"
                     /protein_id="HCY0499676.1"
                     /translation="MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKLIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEALQPDL
                     QENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNDETLVDNNSNSNNENNADII
                     LPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLITSNTTLTVVDADKNNKIVPAQDYLS
                     LKSQITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVQMGINYSIYMDADTIPVSKNDVEFNVTIGNTTTKTTANIQ
                     YPDYVVNEKNSIGSAFTETVSHVGNKENPGYYKQTIYVNPSENSLTNAKLKVQAYHSS
                     YPNNIGQINKDVTDIKIYQVPKGYTLNKGYDVNTKELTDVTNQYLQKITYGDNNSAVI
                     DFGNADSAYVVMVNTKFQYTNSESPTLVQMATLSSTGNKSVSTGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPN
                     LPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGN
                     YKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTN
                     KNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGY
                     TPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKDVKVTLLNEKGEVIGTTKTDENGKYCFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDRDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDRDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DAGKHTPVKPMSTTKDHHNKAKALPETGNENSGSNNATLFGGLFAALGSLLLFGRRKK
                     QNK"
     gene            4575..8039
                     /gene="sdrE"
                     /locus_tag="O0394_001597"
     CDS             4575..8039
                     /gene="sdrE"
                     /locus_tag="O0394_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrE"
                     /protein_id="HCY0499677.1"
                     /translation="MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENNSTNPIKKETNTDSQP
                     EAKKESTSSSTQKQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNN
                     TNNDVTTKPSTSEPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPV
                     NVSKEELKNNPEKLKELVRNDSNTDHSTKPVATAPTSVAPKRVNAKMRFAVAQPAAVA
                     SNNVNDLIKVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKN
                     VIPSDLTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKSRLTLYSY
                     IDKKTVPNETSLNLTFATAGKETSQNVTVDYQDPMVHGDSNIQSIFTKLDEDKQTIEQ
                     QIYVNPLKKSATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNSDQQLPQS
                     NRIYDFSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQ
                     GTSMRTTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQ
                     GTDSKEKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPTGYLPT
                     KVNGTTDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDAN
                     EPGIKDVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTT
                     ADDKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKD
                     VTVTLQNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDA
                     DGGEVDVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDAGKHTPVKPMSTTKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLF
                     GRRKKQNK"
     gene            8158..9630
                     /locus_tag="O0394_001598"
     CDS             8158..9630
                     /locus_tag="O0394_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0499678.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFVMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLSGAGNGIKSFYKTVFNNLTRYKAIVV
                     STEKQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QIEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPQGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(9754..11244)
                     /locus_tag="O0394_001599"
     CDS             complement(9754..11244)
                     /locus_tag="O0394_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0499679.1"
                     /translation="MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRY
                     LYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRI
                     YDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLC
                     EFFYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGD
                     LFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYR
                     AIIVSTEQQKLDVEKRINHTIPVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEK
                     QLHQQIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQE
                     YSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITSDFNGYLITKNDED
                     ALFDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(11526..12404)
                     /locus_tag="O0394_001600"
     CDS             complement(11526..12404)
                     /locus_tag="O0394_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HCY0499680.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
     gene            complement(12417..13082)
                     /gene="bshB2"
                     /locus_tag="O0394_001601"
     CDS             complement(12417..13082)
                     /gene="bshB2"
                     /locus_tag="O0394_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HCY0499681.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLANYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            complement(13096..13458)
                     /locus_tag="O0394_001602"
     CDS             complement(13096..13458)
                     /locus_tag="O0394_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HCY0499682.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQITYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
BASE COUNT         5160 a         2155 c         2292 g         3911 t
ORIGIN      
        1 attatttaaa aatttgatga attaggagta atctaatgct aaacagagaa aataaaacgg
       61 caataacaag gaaaggcatg gtatccaatc gattaaataa attttcgatt agaaagtaca
      121 cagtgggaac agcatcaatt ttagtaggta caacattaat ttttggtctg gggaaccaag
      181 aagcaaaggc tgcagaaagt actaataaag aattgaacga agcgacaact tcagcaagtg
      241 ataatcaatc gagtgataaa gttgatatgc agcaactaaa tcaagaagac aatactaaaa
      301 atgataatca aaaagaaatg gtatcatctc aaggtaatga aacgacttca aatgggaata
      361 aattaataga aaaagaaagt gtacaatcta ccactggaaa taaagttgaa gtttcaactg
      421 ccaaatcaga tgagcaagct tcaccaaaat ctacgaatga agatttaaac actaaacaaa
      481 ctataagtaa tcaagaagcg ttacaacctg atttgcaaga gaataaatca gtggtaaatg
      541 ttcaaccaac taatgaggaa aacaaaaagg tagatgccaa aactgaatca actacattaa
      601 atgttaaaag tgatgctatc aagagtaatg atgaaactct tgttgataac aatagtaatt
      661 caaataatga aaataatgca gatatcattt tgccaaaaag tacagcacct aaacgtttga
      721 atacaagaat gcgtatagca gcagtacagc catcatcaac agaggctaaa aatgttaatg
      781 atttaatcac atcaaataca acattaactg tcgttgatgc agataaaaac aataaaatcg
      841 taccagccca agattattta tcattaaaat cacaaattac agttgatgac aaagttaaat
      901 caggtgatta tttcacaatt aaatactcag atacagtaca agtatatgga ttgaatccgg
      961 aagatattaa aaatattggt gatattaaag atccaaataa tggtgaaaca attgcgactg
     1021 caaaacatga tactgcaaat aatttaatta catatacatt tacagattat gttgatcgat
     1081 ttaattctgt acaaatggga attaattatt caatttatat ggatgctgat acaattcctg
     1141 ttagtaaaaa cgatgttgag tttaatgtta cgataggtaa tactacaaca aaaacaactg
     1201 ctaacattca atatccagat tatgttgtaa atgagaaaaa ttcaattgga tcagcgttca
     1261 ctgaaacagt ttcacatgtt ggaaataaag aaaatccagg gtactataaa caaacgattt
     1321 atgtaaatcc atcggaaaat tctttaacaa atgccaaact aaaagttcaa gcttaccact
     1381 caagttatcc taataatatc gggcaaataa ataaagatgt aacagatata aaaatatatc
     1441 aagttcctaa aggttataca ttaaataaag gatacgatgt gaatactaaa gagcttacag
     1501 atgtaacaaa tcaatacttg cagaaaatta catatggcga caacaatagc gctgttattg
     1561 attttggaaa tgcagattct gcttatgttg taatggttaa tacaaaattc caatatacaa
     1621 atagcgaaag cccaacactt gttcaaatgg ctactttatc ttcaacaggt aataaatccg
     1681 tttctactgg caatgcttta ggatttacta ataaccaaag tggcggagct ggtcaagaag
     1741 tatataaaat tggtaactac gtatgggaag atactaataa aaacggtgtt caagaattag
     1801 gagaaaaagg cgttggcaat gtaactgtaa ctgtatttga taataataca aatacaaaag
     1861 taggagaagc agttactaaa gaagatgggt catacttgat tccaaactta cctaatggag
     1921 attaccgtgt agaattttca aacttaccaa aaggttatga agtaacccct tcaaaacaag
     1981 gtaataacga agaattagat tcaaacggct tatcttcagt tattacagtt aatggcaaag
     2041 ataacttatc tgcagactta ggtatttaca aacctaaata caacttaggt gactatgtct
     2101 gggaagatac aaataaaaat ggtatccaag accaagatga aaaaggtata tctggcgtaa
     2161 cggtaacatt aaaagatgaa aacggtaacg tgttaaaaac agttacaaca gacgctgatg
     2221 gcaaatataa atttactgat ttagataatg gtaattataa agttgaattt actacaccag
     2281 aaggctatac accgactaca gtaacatctg gtagcgacat tgaaaaagac tctaatggtt
     2341 taacaacaac aggtgttatt aatggtgctg ataacatgac attagatagt ggattctaca
     2401 aaacaccaaa atataattta ggtaattatg tatgggaaga tacaaataaa gatggtaagc
     2461 aggattcaac tgaaaaaggt atttcaggcg taacagttac attgaaaaat gaaaacggtg
     2521 aagttttaca aacaactaaa acagataaag atggtaaata tcaatttact ggattagaaa
     2581 atggaactta taaagttgaa ttcgaaacac catcaggtta cacaccaaca caagtaggtt
     2641 caggaactga tgaaggtata gattcaaatg gtacatcaac aacaggtgtc attaaagata
     2701 aagataacga tactattgac tctggtttct acaaaccgac ttacaactta ggtgactatg
     2761 tatgggaaga tacaaataaa aacggtgttc aagataaaga tgaaaagggc atttcaggtg
     2821 taacagttac gttaaaagat gaaaacgaca aagttttaaa aacagttaca acagatgaaa
     2881 atggtaaata tcaattcact gatttaaaca atggaactta taaagttgaa ttcgagacac
     2941 catcaggtta tacaccaact tcagtaactt ctggaaatga tactgaaaaa gattctaatg
     3001 gtttaacaac aacaggtgtc attaaagatg cagataacat gacattagac agtggtttct
     3061 ataaaacacc aaaatatagt ttaggtgatt atgtttggta cgacagtaat aaagacggca
     3121 aacaagattc aactgaaaaa ggtatcaaag atgttaaagt tactttatta aatgaaaaag
     3181 gcgaagtaat tggaacaact aaaacagatg aaaatggtaa atactgcttt gataatttag
     3241 atagcggtaa atacaaagtt atttttgaaa agcctgctgg cttaacacaa acaggtacaa
     3301 atacaactga agatgataaa gatgcagatg gtggcgaagt tgacgtaaca attacggatc
     3361 atgatgattt cacacttgat aatggatact acgaagaaga aacatcagat agcgactcag
     3421 attcggacag cgactcagat tcagacagag actcagactc agatagtgat tcagactcgg
     3481 atagcgattc agattcagac agcgattcag attcagatag cgattcagat tcagacagag
     3541 actcagatag tgattcagac tcagatagcg actcagattc agacagcgac tcagattcag
     3601 acagcgactc agactcagat agtgattcag actcagatag cgactcagat tcagacagcg
     3661 actcagactc agacagcgac tcagactcag atagtgactc agattcagat agcgactcag
     3721 attcggacag cgattcagac tcagatagcg actcagattc agatagcgat tcggactcag
     3781 atagcgactc agattcagat agtgattcag actcagatag cgactcagat tcagacagcg
     3841 actcagattc ggatagcgac tcagattcag acagcgactc agactcggat agtgattcag
     3901 actcagatag cgactcagac tcagatagcg attcagattc agatagcgac tcagactcag
     3961 acagcgattc agactcagac agcgactcag actcagatgc aggtaagcac acacctgtta
     4021 aaccaatgag tactactaaa gaccatcaca ataaagcaaa agcattacca gaaacaggta
     4081 atgaaaatag cggctcaaat aacgcaacgt tatttggcgg attattcgca gcattaggat
     4141 cattattgtt attcggtcgt cgtaaaaaac aaaataaata atacagctta taccaggtcc
     4201 gtgaggcctg gttttttatt tcaaaattta tgaaatgtat cttatgtttg agattaaata
     4261 gtgcaatttt ttaaaaaata taacataaat caacataaat ttaattcatc tttacattaa
     4321 aagtacaaac gaataagaaa agcaattaaa cacagtatca aaccatttaa aacaattgat
     4381 ataaaaaaca aacgaataat taaaaagtac tagaattaat ttattcaaac caaagtgtaa
     4441 aataacagtc ttagataaat aaatttattt aaagtattgt gctttatcta aaaatgtatt
     4501 acgatgggaa tacaaatttt tatatgtaaa aatattaatt ttttgcaaat attgatattt
     4561 taaaggagat ttatatgatt aacagggata ataaaaaggc aataacaaaa aagggtatga
     4621 tttcaaatcg cttaaacaaa ttttcgatta gaaagtatac tgtaggaact gcatcgattt
     4681 tagtaggtac gacattgatt tttggtctag ggaaccaaga agctaaagct gctgaaaaca
     4741 ctagtacaga aaatgcaaaa caagatgatg caacgactag tgataataaa gaagtagtgt
     4801 cggaaactga aaataattcg acaacagaaa ataattcaac aaatccaatt aagaaagaaa
     4861 caaatactga ttcacaacca gaagctaaaa aagaatcaac ttcatcaagt actcaaaaac
     4921 agcaaaataa cgttacagct acaactgaaa ctaagcctca aaacattgaa aaagaaaatg
     4981 ttaaaccttc aactgataaa actgcgacag aagatacatc tgttatttta gaagagaaga
     5041 aagcaccaaa taatacaaat aacgatgtaa ctacaaaacc atctacaagt gaaccatcta
     5101 caagtgaaat tcaaacaaaa ccaactacac ctcaagaatc tacaaatatt gaaaattcac
     5161 aaccgcaacc aacgccttca aaagtagaca atcaagttac agatgcaact aatccaaaag
     5221 aaccagtaaa tgtgtcaaaa gaagaactta aaaataatcc tgagaaatta aaagaattgg
     5281 ttagaaatga tagcaataca gatcattcaa ctaaaccagt tgctacagct ccaacaagtg
     5341 ttgcaccaaa acgtgtaaac gcaaaaatgc gctttgcagt tgcacaacca gcagcagttg
     5401 cttcaaacaa tgtaaatgat ttaattaaag tgacgaagca aacaatcaaa gttggcgatg
     5461 gtaaagataa tgtggcagca gcgcatgacg gtaaagatat tgaatatgat acagagttta
     5521 caattgacaa taaagtcaaa aaaggcgata caatgacgat taattatgat aagaatgtaa
     5581 ttccttcgga tttaacagat aaaaatgatc ctatcgatat tactgatcca tcaggagagg
     5641 tcattgctaa aggaacattt gataaagcaa ctaagcaaat cacatataca tttacagact
     5701 atgtagataa atatgaagat ataaaatcac gcttaactct atattcgtat attgataaaa
     5761 aaacagttcc aaatgagaca agtttgaatt taacatttgc tacagcaggt aaagaaacaa
     5821 gccaaaatgt cactgttgat tatcaagatc caatggtcca tggtgattca aacattcaat
     5881 ctatctttac aaaattagat gaagataagc aaactattga acaacaaatt tatgttaacc
     5941 cattgaaaaa atcagcaacc aacactaaag ttgatatagc tggtagtcaa gtagatgatt
     6001 atggaaatat taaactagga aatggtagca ccattattga ccaaaataca gaaataaagg
     6061 tttataaagt taactctgat caacaattgc ctcaaagtaa tagaatctat gattttagtc
     6121 aatacgaaga tgtaacaagt caatttgata ataaaaaatc atttagtaat aatgtagcaa
     6181 cattggattt tggtgatatt aattcagcct atattatcaa agttgttagt aaatatacac
     6241 ctacatcaga tggcgaacta gatattgccc aaggtactag tatgagaaca actgataaat
     6301 atggttatta taattatgca ggatattcaa acttcatcgt aacttctaat gacactggcg
     6361 gtggcgacgg tactgttaaa cctgaagaaa agttatacaa aattggtgac tatgtatggg
     6421 aagacgttga taaagacggt gttcaaggta cagattcaaa agaaaaacca atggcaaacg
     6481 ttttagttac attaacttac ccggacggta ctacaaaatc agtaagaaca gatgctaatg
     6541 gtcattatga attcggtggt ttgaaagacg gagaaactta tacagttaaa ttcgaaacgc
     6601 caactggata tcttccaaca aaagtaaatg gaacaactga tggtgaaaaa gactcaaatg
     6661 gtagttcggt tactgttaaa attaatggta aagatgatat gtctttagat actggttttt
     6721 acaaagaacc taaatacaac ttaggtgact atgtatggga agatactaat aaagatggta
     6781 tccaagatgc aaatgagcca ggaatcaaag atgttaaggt tacattaaaa gatagtactg
     6841 gaaaagttat tggtacaact actactgatg cctcgggtaa atataaattt acagatttag
     6901 ataatggtaa ctatacagta gaatttgaaa caccagcagg ttacacgcca acggttaaaa
     6961 atactacagc tgatgataaa gattctaatg gtttaacaac aacaggtgtc attaaagatg
     7021 cagataatat gacattagac agtggtttct ataaaacacc aaaatacagt ttaggtgatt
     7081 atgtttggta cgacagtaat aaagacggca aacaagattc aactgaaaaa ggtatcaaag
     7141 atgtgacagt tacattgcaa aacgaaaaag gcgaagtaat tggaacaact aaaacagatg
     7201 aaaatggtaa atatcgtttc gataatttag atagcggtaa atacaaagtt atttttgaaa
     7261 agcctgctgg cttaacacaa acagttacaa atacaactga agatgataaa gatgcagatg
     7321 gtggcgaagt tgacgtaaca attacggatc atgatgattt cacacttgat aacggatact
     7381 tcgaagaaga tacatcagac agcgattcag actcagatag tgactcagac agcgactcag
     7441 actcagacag cgactcagac tcagacagtg attcagattc agacagcgac tcagattcag
     7501 atagcgactc agattcggac agcgattcag actcagatag cgactcagat tcagatagcg
     7561 attcagactc agacagcgac tcagattcag atagcgattc ggactcagac agcgattcag
     7621 actcagatag cgactcagac tcagacagcg actcagattc agatagcgat tcggactcag
     7681 atagcgactc agattcagac agcgattcag actcagatag cgactcagac tcagacagtg
     7741 attcagattc agacagcgac tcagactcag atagcgactc agattcggac agcgactcag
     7801 actctgatag cgactcagac tcagacagtg attcagacag cgattcagac tcggatgcag
     7861 gaaaacatac acctgttaaa ccaatgagta ctactaaaga ccatcacaat aaagcaaaag
     7921 cattaccaga aacaggtagt gaaaataacg gctcaaataa cgcaacgtta tttggtggat
     7981 tatttgcagc attaggttca ttattgttat tcggtcgtcg caaaaaacaa aacaaataat
     8041 acaatatgac ccaggtcctt gtggcctggt ttttttataa ttacacatgc aatagatgta
     8101 tttttcatat aaaataaaca ataaagatac ggaataaaac ttatatgagg cgataatatg
     8161 aattacattt taggaacaat tttagaaagt aaaattacag gtgtagaaaa agcgcaaata
     8221 aatagattga agttgttcaa acaacacggc atatcttcaa aatgtgtata tgttaaatgg
     8281 aatccttatt catacacata tgcgaagcaa catcagattg aaaatgatgt atttacaatg
     8341 tatgactatt ttcaaaaagc aatcaattat aaaaagacaa agcaagttaa ctggatacag
     8401 tattgggaaa agtcatgtag gtacacattg aaatttgtgg aaaattcaaa tgatgtcaga
     8461 atatatgatg aagagcaatt tgtaatgtat gctcattttt tagataaaca gtatcatcaa
     8521 ttaaattatg tgaattattt tgatcataaa agaagaaaag taaaacgcga attgtatgat
     8581 ggaagaggct ttttaagttg ttctcgaatt ttaggtgaag gacaacggat tgtactcgaa
     8641 aattactata cacctaatgg ggaaatcgtc atccaaaaat attttgacga tataaaaggg
     8701 aaaaacacgc tcacaaaggt tatcttaaat gaagaccagc atcaacaatt ttttgataca
     8761 gaagatgaat tagttcaata ttttctccat caattatgta aaaataatga tcaaatcata
     8821 ttagatcgtc ctcatgaatt aggaaatgtt atagcgggat taaatcaaag tattccagtt
     8881 attgttgtac tccatagtac acatttatcc ggtgccggta atggtataaa aagtttttat
     8941 aaaacagtgt ttaataattt aacacgttat aaagcgattg ttgtatcaac agaaaagcaa
     9001 tgccaagata tttcacaata tattgaaaat aaaataccag ttatcaatat tccggttggc
     9061 tacgtggcaa atttaaagta tcaatttgac atcaatcaaa aggagaaaaa tcatatcata
     9121 tcaattgctc gcctcgttga aaataaacaa attaaacatc aaattgaagt aatcaagcaa
     9181 ttagtaacaa aacatcccaa tattcaattg aatatttatg gacatggaaa tggtttgtca
     9241 gaatatcgac aacttgtaga agattatcat ttatcggaac atgttaaatt tcatggtttt
     9301 aagacgcata ttaatgaaga gattgctaaa gcagaactga tgttatcgac aagtaaaatg
     9361 gaaggttttg gcttagcaat tttagagtcg ctttcagtag gtacaccagt gatcagttat
     9421 gatgtagatt atggtccatc agaactgatt caagatggat ttaatggcta tttagtacct
     9481 caaggtgaca tcaatcaaat ggttgaaaag gtcgaccaat tactaaataa tactcaaaaa
     9541 ttgcaacagt tttcaattaa tagcatagaa tctgcacaac agtacaatgc aactactatc
     9601 agtacaaagt ggcaaaatat tttaaactaa gtcaaaagaa aaaagcattt tcctagaatc
     9661 atctatttaa caaacctcgt tcatacgaac ttacatgttt agttaaatga cataggaaaa
     9721 tgctattttt gtaaaaataa aggtaaagta catttatata agacgaacaa attggtccca
     9781 ttgtttaatt aacgacgctt tactatattg ttgtgctttt gccaaactac cttttgacag
     9841 tcgttgctgt acttcaggat gatcaatcac atattttact ttatcaaata gggcatcttc
     9901 atcattttta gtaattaaat aaccattgaa atctgaagta atcagttcgt taggtccata
     9961 tttaatatca taactaataa ctggaacacc atgtgctaaa gattcaagta gcgctaaaga
    10021 gaaaccttcc atgttacttg ttattaaact caaataggca tcgctatatt cttggtctag
    10081 attgcttaaa aagccgcgta agtaaacatg attttccaat ccatattttt gtatcaattc
    10141 atttaatttt ttactttcag aaccaaaacc atacatatga agctctattt ttgggacata
    10201 cgatactaag cgtttaatta attcaatttg ttgatgtaat tgtttttcag gtgaataacg
    10261 agcaacggaa attaatttaa cactgcgctg atctaatgtt tggactggtg tatcaattgt
    10321 ttcactatag ccgacaggaa tattaacaac tggaatagta tggttaatac gtttttcaac
    10381 atctaatttt tgctgctcag tagaaacgat aattgcacga tatcgagata aattttcaaa
    10441 catcgcttta tatacatttt taaatggcga tgaatctaat gcatcaatat ttttaatgtg
    10501 tgtactgtga agcacagcta ctactgggat tgactcaggc gttaagttga aaataggtgc
    10561 tgtgtacaca ttacgatcac tgaaaaataa atccccatgt tgatatagtt gtttaatgaa
    10621 aaatgcgcct aattccgttt cattattaaa gaaatattgt ttgttagcat agtaaacaat
    10681 aattttttgt acttctggtt tgccatcctt gtaagaaaaa tacttttcta attttgtgtc
    10741 accttctgga ttatagaaaa attcacataa tgtttgttgt ttatcaacaa gaatcctact
    10801 acaacttaaa aagccacgca catcataaaa atcacgtttt acttttcgtc tttgactatc
    10861 aaaatgattt acataatcta atatacgata tttaggatct tgaaaatggg catacattaa
    10921 gaaacgctct tgatcatata ttctaaagtc atgactattt tcaacatgtt ttaaagtata
    10981 atgacattca tcagtccaat acgacaacca gtcaaatggt tcattgcgtt ctaaatatgt
    11041 tgcttcttgg aagaaatcat acatattaat atagtcagaa ctagtaatat aattttgggc
    11101 atttctatat aaatatctat tccatgacag aaatacacat tgcgctggtc ttcccatttc
    11161 tttaaataaa tttaaacgat taataattgc tttctctatc ccagttaaat taacacctaa
    11221 actattacct acaaaataat tcatttacaa caccacttat atctattttt tataattata
    11281 tcacataata tttaattact tcttttaact ggaagatgtg tttatttata aaacaacaaa
    11341 ttttgatatt tataatgata gtagttattc aatcaactac gaccaatata tcattgtaga
    11401 gcttaggata ttgatttatg actcaggcac atcaaatgag aagatttata aaagagatat
    11461 acaactctag aaggtataat aaaaacgcgc aactaatgtt acgcgtttga attaatcata
    11521 tgatattatt tgcgatactt taatttagcg aaagcatcat gttgatggat agactcttca
    11581 ttacgacatt cgatatcgaa accgtctaac caatcaaatt caactaagtc cgcggcaatt
    11641 aaacgaatta agtcttcgac aaaacgtgga ttttcatatg cacgctctgt cacacgtttt
    11701 tcatcaggac gttttaaaat agggtataga attgaacttg cattagcttc cattgcatct
    11761 aaaattttat ttttatagtc atcaactatg tcttgatctt tattaatata tgttttaaca
    11821 gtgacaacac cacgttggtt gtgcgctgaa tactcactta tttcttttga acaagggcat
    11881 agcgttgtga cagttgcttc aatagtaagt tctttacgtg taactttatc accgtcaatt
    11941 gctaatccat aagtgacatc ggcattacca actgctttaa tatttgtggt tggactatag
    12001 cgatcaaaga accatttccc agaaacatca acgcctgccg cattttgttt catattcgtt
    12061 tgtaaagtgc gtaacacctg ataaagtgta ttaaattcaa gttcaatacc attatcatag
    12121 tgcttttcaa cactttcgat tatacggctc atattaatac ctttttcgtc ttttgttaaa
    12181 cttgttgaaa aactaaatgt gccagctgtt tgatactggt caacaagtac agggtacact
    12241 aagtttttaa taccaacttc ttctatttca aataaaaaat ctttatgtgt actttgtaaa
    12301 tctgtcattt cgttcttagt agtaggtttc gtgccttcaa taggatctac ggaaccaaag
    12361 tgtttccaac gaccttctcg tgtcgataaa tcaaattcag tcattttttt cctccgttaa
    12421 gatttaaagt gatatgtcca atatggttcg actgttaaaa agctgtgttg tttaccatcg
    12481 atttcaggac ttgctaattg ttttaaaaat ggacctgttt gagaagcatg tgcttcaaat
    12541 gccttaattt taagttcttt aaaatctgta atatcatttt gaatatcagg ttctccaaga
    12601 gcttcggttg catcattact gaacgcaact aaagttaaac gagggcgttc ttctttaggc
    12661 atgcgttcaa ccgttcgaat tacagcgtct gctgttgctt cgtgatcagg atgtactgca
    12721 tatccaggat aaaatgaaat aatcaatgat ggatttgtat catcgattaa agatttaatc
    12781 ataccatcta tatgttcata gggttcaaat tcgacagttt tgtcacgtaa acccattttt
    12841 cttaaatcag taataccgat aactttacaa gcttcttcta gttcacgctc acgaatactt
    12901 ggtaatgatt cgcgtgttgc aaatggggga ttacctaaat ttctgcccat ttgtcctagg
    12961 gttaaacatg catatgttac aggtatgcct ttttggatat aatttgctaa tgtgcctgca
    13021 gatgagaagg tttcatcatc aggatgtgga aatattacta atacatgtct ttcgtcagtc
    13081 atgttgatgc ctcctctata aattaaatgg tcgctcacta atttgaagtg ctgcagcgag
    13141 ttgaccttcg taattaaaac ctgcaattaa aaattcatca tgctcattga cctcaaaatg
    13201 cgttagacct tgtacataaa cccaaccacc atttgatagt ttaagaccaa tgcgataagg
    13261 ttctttatta ccacctttta gttgtgcatg cgtatatgtt atttgtatgt ttcttaaaaa
    13321 agtaccagca ttaaaaacac gttgatcgaa atggttcgca taggccccat ttgtcgtttc
    13381 aacatgcaga tacacaggtt tatgttcaaa agaagcaagt aaatctataa cttcttgttc
    13441 tttaattggt tccaacacgt tcactcctta cactatcaat gtgtttatct ttctatttta
    13501 ctaaaaacta ttcgataa
//
LOCUS       DAKTLH010000068        13479 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.068, whole genome shotgun sequence.
ACCESSION   DAKTLH010000068 DAKTLH010000000
VERSION     DAKTLH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13479
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.068"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(186..689)
                     /locus_tag="O0394_001603"
     CDS             complement(186..689)
                     /locus_tag="O0394_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCY0499683.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKYNDVKAFYTHGHLYQVNRTRDLLAEKGLELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVIIDDQTLHGTINFKNRHHE
                     TISHTTF"
     gene            complement(682..1269)
                     /locus_tag="O0394_001604"
     CDS             complement(682..1269)
                     /locus_tag="O0394_001604"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCY0499684.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEQKGQISHRRNAINLLQAFLEGDKNV"
     gene            complement(1281..2081)
                     /gene="racE"
                     /locus_tag="O0394_001605"
     CDS             complement(1281..2081)
                     /gene="racE"
                     /locus_tag="O0394_001605"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCY0499685.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(2317..3132)
                     /gene="sdhB"
                     /locus_tag="O0394_001606"
     CDS             complement(2317..3132)
                     /gene="sdhB"
                     /locus_tag="O0394_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HCY0499686.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(3132..4898)
                     /gene="sdhA"
                     /locus_tag="O0394_001607"
     CDS             complement(3132..4898)
                     /gene="sdhA"
                     /locus_tag="O0394_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCY0499687.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(4950..5564)
                     /locus_tag="O0394_001608"
     CDS             complement(4950..5564)
                     /locus_tag="O0394_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HCY0499688.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(5888..7669)
                     /gene="uvrC"
                     /locus_tag="O0394_001609"
     CDS             complement(5888..7669)
                     /gene="uvrC"
                     /locus_tag="O0394_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCY0499689.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            7835..7930
                     /locus_tag="O0394_001610"
     CDS             7835..7930
                     /locus_tag="O0394_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499690.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(7993..8307)
                     /gene="trxA"
                     /locus_tag="O0394_001611"
     CDS             complement(7993..8307)
                     /gene="trxA"
                     /locus_tag="O0394_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCY0499691.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(8480..10828)
                     /locus_tag="O0394_001612"
     CDS             complement(8480..10828)
                     /locus_tag="O0394_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCY0499692.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSIKGTKPIFKEDRTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(10838..12550)
                     /gene="polX"
                     /locus_tag="O0394_001613"
     CDS             complement(10838..12550)
                     /gene="polX"
                     /locus_tag="O0394_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HCY0499693.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITIDDING
                     DIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNT
                     KLKK"
     gene            complement(12623..13144)
                     /locus_tag="O0394_001614"
     CDS             complement(12623..13144)
                     /locus_tag="O0394_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCY0499694.1"
                     /translation="MVIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(13145..13411)
                     /gene="zapA"
                     /locus_tag="O0394_001615"
     CDS             complement(13145..13411)
                     /gene="zapA"
                     /locus_tag="O0394_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCY0499695.1"
                     /translation="MTQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
BASE COUNT         4090 a         2602 c         1887 g         4900 t
ORIGIN      
        1 gattcaacca tatagggtta tcaacaatca cattctccaa aattacaatt cttttcataa
       61 cataaatgtg ttattcattc caattatctt ataaaattgt cagaaatact tttaaacaaa
      121 cattcatgac acgacctcct agttaaatca gtctataaac taaaaaaagc catagctact
      181 tctatttaaa aagtagtatg actgattgtt tcgtgatgtc gatttttgaa attgatggtg
      241 ccatgtaaag tttgatcatc aataataact tcagcatatg tttcttccat tgaacttcta
      301 gattgagata tacttccagg attaataaca tgaacaccat taatatactc atattttgcc
      361 acatgtgtat gtccataaaa tgcaaacaaa caacctaatt caagtccttt ttcagctaat
      421 aaatctcttg ttcgattgac ttgatataaa tgtccatgag tataaaatgc tttcacgtca
      481 ttatattttg cgaccgcttc attttcaaat tctgggtaaa aatcacaatt gccttttact
      541 ctattaaata agctaagttc cgtatcatca tacgcgaatt ctgaatctcc taaatgtaaa
      601 tatacatctg catctgggtg catttcataa atttgatata aaacgcctga ttcagtatgg
      661 ttatcactca caataatcca tttagacatt tttatcacct tcaagaaaag cttgaagtaa
      721 attaatcgca tttcgtctat gactaatttg ccctttttgt tcttttgaaa gttgagccat
      781 ggttttatct aatttcggta cataaaatat cggatcatat ccgaaaccat tttcgccata
      841 ttttccatct gcaatttcac ctgaaacagt acctttaaat acttttgttt ccatatcagg
      901 gccactcata cttatgacac aaacaaattg cgcacgacga tcagttgtat taccaagctt
      961 atttaataat ttttcaatat ttgcttcatc gcttttattt tcaccagcat aacgtgcaga
     1021 gtatatacct ggctcaccat ttaatgcaaa aacttctagt ccactgtcat cagctatgac
     1081 cgttttattc aatgcttttg cagcagcttc tgattttaat atagcatttt cttcaaatgt
     1141 tgatcctgtt tcttccacat caaaatctgg tattagttct gaaataccta ttacgtggta
     1201 atctggaaat attactttaa agtcatttat tttcccttga ttattcgatg caataacaat
     1261 ctctttcatt aaaaatcctc ctagtcattc actgatatac gttccacatt gacagataaa
     1321 tttaaccact ctttgataat gttagtaatg tgagtaggat cacctgttgc aaaaaatcga
     1381 tgatctggat gttcagtata acttgcatgt tcattactaa atgttagcaa tgcactaact
     1441 tcacgagccg tttctaatcc agacgaaatc actgtctttt taccaccaaa ataatcatag
     1501 ataggtttat agagcaatgg atagtgggta catcctaaaa tgacagtatc agactcacta
     1561 ttacgccaac gtttcagtgt ttgatgaatg acaatgcttg taattgttgg atcactatat
     1621 ctcatttgtt ctacaagtgg cacaaaacct ggacaggcaa cgccatgtac ctctacatgt
     1681 ggattgatac gtttaatatg cgtacgatat gcttcagatt taattgtgcc ttccgttcct
     1741 agtactaata cattttgatt tctagtagtc attattgctg ttctagcacc tggttcaatt
     1801 acgccaatca ctgggattga taaggtcttt tgtaaatatt ctaaagctac agcagttgct
     1861 gtattacaag caatcacgag catttttata tcaaattcca ttaatttacg agcgatttca
     1921 actgtatatt gttttacttg ttctcctggt cttggcccat atggacatcg tccaatatca
     1981 cctaagtaat aaatcgtctc atttggcaac tgacgcataa tttctttagc tactgtcaaa
     2041 cctccgacac cagagtctat tacacctatt ggtttattca tattcggtca tccttacttt
     2101 taatttaatt tttagtttat cataactaag cattggattt tagtattatg cactgtgttt
     2161 accatttttg tcattataat atttatttta aatcagccca ctatcatatt gtcatgtaat
     2221 ctgcttatta aaaaaatccc ttccaagtta ttgtgtatct ccattcaatt taattttgaa
     2281 aggaacataa cattttaact caaaagggat taatttttat tctacttcat ggtctgaacc
     2341 aaagaatgat ttaaacatgt ggaatgttgt ttctctgttc attgctgcaa tggatgttgt
     2401 taatggaata cctttagggc aagcattaac acagttttgt gaattaccac actgctgtaa
     2461 gccaccagta cccattaatg catttaaacg ttcatcttta gtcatagatc ctgttgggtg
     2521 caaattaaac aaacgaactt gcgagattgc ttgtgcacca acgaatttat tattttcagt
     2581 aacattagga caaacctcta aacatacacc acatgtcata catttagata attcataagc
     2641 tgtttgacgt tttttctctg gcatacgtgg tcccggacct aaatcatacg ttccatcaat
     2701 tgggatccat gctttcatac gttttaagtt atcgaacatt ctagaacgat caacttgtaa
     2761 gtcacggata actgggaaag tattcattgg ctctaaacga ataggttgtt ctaattgatc
     2821 aacaatcgca gaacaagatt gtcttgcacg accattgata accatagaac atgctccaca
     2881 tacttcttct aagcagttca tatcccagac aacaggtgtt gttttttcac ctttaatatt
     2941 aactgggtta cgtctaattt ccattaaaca agcaatgacg tttaaatttt cacgatatgg
     3001 aatttcaaat gtttcttcat aaggcttaga atcacttgta tcttgtcgtt taataattaa
     3061 ttttactgtt ttttgtttcg gtttagattg tgtttcatgt tgtggagtgt ttttcactga
     3121 ttgttcagtc attatttttt acccccttta gacttacttg tgtaatcacg tttacgaggt
     3181 ggtattaaac tcacatcgac gtcatcataa gtaaactgcg gtttttcaaa tgcgccttgg
     3241 aatgaggcca ttgtcgtttt taaccactct tcatcattac gctctgggaa ttctggttta
     3301 taatgggcac cgcgtgattc gttacggtta tatgcaccaa tcgtaataac acgtgcaagt
     3361 actaacatgt tccatagttg acgggtaaag aataccgctt ggttactcca agtttgagta
     3421 tcttccatat caatatcttc ataacgtttc atcaattcaa caatcttttt atctgtttct
     3481 aacagttttt cattttcacg aacaacagtt acatttgctg tcataatttc accaagttca
     3541 cggtgtaatt tatatgcatt ttctgtaccg cgcatagcta ataatttatc aaaacgttct
     3601 tgctcttcag ctttacgctt ttcaaaaata ctttcgtcca tatcagtata tgatcgatca
     3661 atatttgaaa tataatcaat cgcgtttgga cctgctactg taccaccata aatcgctgat
     3721 aacaatgaat tggcacctaa gcggttacca ccatgttgag agaagtcaca ttctccagct
     3781 gcaaataacc ctttaatatt tgtcatttga tcataatcta catatagacc acccattgaa
     3841 tagtgaacag ctgggaaaat cttcattggt actttgcgtg ggtcatcacc agtgaatttt
     3901 tcataaatct caatgatacc acctagtttt acatctaact catgtggatc tttatgtgac
     3961 aaatcaagat ataccatgtt ttcgccattt atacctaatt tttggttaat acatacatcg
     4021 aaaatttcac gcgttgcgat atcacgaggt actaagttac cataatcagg atatttctct
     4081 tctaagaagt accaaggctt accatcttta tatgtccaaa ttcgtccacc ttcaccacgt
     4141 gctgattcac tcattagtcg cagtttatca tcaccaggga ttgcagtagg atgaatttga
     4201 atgaactcac cattagcata aatagcgcct tgttggtaaa caatggaagc cgctgatcct
     4261 gtattaatca ttgagtttgt tgttttaccg aaaataatac cagggccacc cgttgccata
     4321 ataactgcat ctgaaccaaa tgtttcaatc tcagcagttg tcatattttg tgcaacgata
     4381 cctcttgcac tatcatcgtc acctttaact atgccaagga attcccatcc ttcatacttc
     4441 gtaactaatc catctacttc atatgcacga acttgttcat ccaatgcata taataattgt
     4501 tgtccagttg ttgcccctgc atatgctgtt ctgtgatgta atgtaccacc gaaacgtcta
     4561 aaatctaata gaccttcatt tgttctattg aacattacgc ccatacggtc taataaatga
     4621 ataattttag gtgctgcctc tgtcatcgct ttaacaggtg gttggtttgc aaggaaatcg
     4681 ccaccataca ctgtatcatc aaagtgaatc caaggagaat cgccttcccc tttagtattg
     4741 accgcaccat taatgccacc ttgggcacaa acagagtgcg aacgctttac tggtacaact
     4801 gagaacaaat ctacatgtgc acctttttct gccgctttaa ttgttgacat taagcccgct
     4861 aggccacctc cgacaacaat aagatgtttc tctgccataa aaatttcact cccctaaatt
     4921 ttcaatctat atttgttaaa tgcgatgtat tacataaagg caataattgc agtaacacca
     4981 atatacgaaa taactaaaaa tacgattaat gaaacccatg taaatactcg ttgtgatttt
     5041 ggagattgaa gtccacccca agtaactaag aatgaccata agccatttgc aaagtggaac
     5101 acaacagcaa taatacaaat aatataaaat attgcccatc caggatgttg caatgtttcg
     5161 tgcattaaat cgtaattcac ttctttgccg taaaatgctt tttgtaaacg tgtttgccat
     5221 aaatggatac caataaagat aaatgttaag ataccactca ctctttggaa gaagaacatc
     5281 cagtttctaa aaatcgagta atgtccaaca ttttcttttg ctgtaaatgc aatgtgtata
     5341 ccaaacaaac cgtgatataa caacggaatg tatataaata aaaattctac aataattaga
     5401 aatggtaatg attccataaa gttagatgcc ttattaaacg cttcagcacc ttgtgttgct
     5461 tggtgattca ctaataaatg aacgaccaaa aatgcaccta ttgggataat acctaataac
     5521 gagtgaatac gtcttagata aaattcattt tttgattgag ccaaaaggag tcccccctgt
     5581 gaacgaatat ttaatttatt gagctattta tattaaacgt acgcttaacc ccctaaagtg
     5641 atatcgtttc tagcgttgtc attatcatta agcgaaacat tttaaagaca aatacacact
     5701 gcacgatcac caaactgcat gtcgaacaat gtaacatttg gattcgatat ttaaaattgc
     5761 ttgtgatgat aaactttctc atttagaaaa cgcttccacg tacattcaaa aaaataactt
     5821 tgttaaccat attgtaacat tatttcatat attttggggc atgagaatga ttctcacgcc
     5881 cagtaattta tttatgcaat tgttcatgta ggttctttgc gacgttttca ggaataccta
     5941 tatttttaaa atcttcaagt gtagcttcct tcattttctt gattgaaccg aatgaacgca
     6001 ataataatgt tttacgtttg ttaccgatac catctatatc atcaagtatt gatttcaagc
     6061 ctgtcttttg acgtgtttgt ctatgaaatg tgattgcgaa tctgtgaacc tcatcttgga
     6121 tacggtgcaa caaataaaat gcctggctat ttttcttcag tggtacaatt tctgcactag
     6181 cgccatataa taattcagat gtttggtgtt tatcattttt ctgcaaacct gcaacaggga
     6241 tatcaagacc taattcgttt tgtagcacat caataacccc gttcatatgt cctttaccac
     6301 catctactat tattaaatca ggtaatggta atccttcgtt taaaacgcga gaatatcgtc
     6361 gtcttactac ttctctcatt gatttgtaat catctggacc tttaaccgtt ttgattttat
     6421 actttctata atttttctta tctggtttac cgtcgacaaa tgtaaccatt gctgacactg
     6481 gatccacacc ttgaatatta gaattatcga atgcttcaat tctaattggt gtttgaattc
     6541 ccatttgtgt tccaagttct tcaatagctt taatcgttct ggactcatca cgtgatatta
     6601 attcaaattt attatttaag gatactttag cgttatgtgc agctaggtca accatatctt
     6661 ttttgggacc tcgcgcgggt tgaacgattt tagtgtccac aacagattga atcatttctt
     6721 tatccaaatt acgtggtaca tgaacttcct taggtaaaat atgttggttt aagctataaa
     6781 attgtccaat aaatgtataa aattcttctt cttctgtttg ctgtaatgga atcatcgttg
     6841 tatctcgctt tatcatatta ccttgtcgta taaagaaaac ttggatacac atccatcctt
     6901 tatcaacact ataaccaaag acatcacgaa tcgttttatc tgatgacata attttttgtt
     6961 tgtttgtcag attttgaata tgttgaatta aatctctata ttctttagcc cgttcaaaat
     7021 caagtgattc acttgcagtt aacattcgct cttctaaact ttttaaaatt gttttgtctt
     7081 ccccattcag aaaatcagta atttccttcg tcatttgtgc gtatttactc aaatcaacgt
     7141 catatacaca tggtcctaaa cattgtccaa tatggtaata aagacataat ttatctggca
     7201 tcttatcaca tttgcgatat ggatatattc tgtctaataa ctttttagtt tcttgagcag
     7261 aatatgcatt cggatacggt ccgaaatatt tgccagtacc ttgttttaca gttctcgtca
     7321 ctagtagtct aggatatttc tccttcgtaa ttttaataaa tggataactt ttatcatcct
     7381 ttaataatat attatatctt ggttgatatt gtttaatcag attcaattcc agtaaaagtg
     7441 attctgtttc acttgacgtg acaataaact caaagcgacg tatttcacca accagtctcg
     7501 ttgttttagc atcatgagca cccgtaaaat atgatcgcaa tcgatttctt agctttttag
     7561 ctttgccaac atatatcact tgatcattac gatctttcat taaatagcat cctggttcca
     7621 taggtacgac atttaattta tttttaattc gttgcttata gtcttccaaa aatatccccc
     7681 cttctaattt taaaatgaat gatgctacaa aagtgcttca aatttatcac cactaattct
     7741 agttaataat caacatcttc gagtatgact tttcatgcac atcatgtaat attgaacatg
     7801 gttttatcaa accaactgct acaaatctat cagcatgcta gattatcatt taacgttcat
     7861 agatttatta atcccaccat tctcaagtcg taaacaatat gatgacttat cattcaactt
     7921 tttatattaa aaacacaaac aaaaactgga gcaattcatt aaagaaatgc cccagtcgtc
     7981 attggttgta acttataaat gtttatctaa aacttcagct aagttttctt ttggttggaa
     8041 accaacaact ttatcaactg gttgaccgtc tttaaagacg attaatgttg gaatactcat
     8101 cacttcatat ttagctgcag ttgatggatt ttcatcaaca tctaatttta aaatgtcagc
     8161 tttaccttca tagtcagctg ctaattcttc taataccgga gcgatcattt tacatggacc
     8221 acaccatgtt gcccaaaaat ctactagttg tacaccagat tctacttttg aatcaaaatc
     8281 tgcatctgtt acttttacga ttgccataaa tgccaatcct ccttaattat tattatgatg
     8341 ttcaaattat atcagataac atgcttcatg tatttctatt tgctcaattt ttatgcttat
     8401 ttcaaataac atgtttgaaa tgtattcttg ttgctttaca ttattttaac tgcagctatt
     8461 taatttatca aattataatt tattttagtg ttgcaacggt aacgccaaat ccaccttcgc
     8521 ttggcatacc acctctaaag tcactaacac ttttatgctt tttcaaatgt tgttgtacac
     8581 ctttttgaag tgcacctgta cctttaccat gaatgatata aacttgttcg taattactta
     8641 aaacggcttg atctaaatat tgatctagtt caattaaagc atcctcataa cgatagcctc
     8701 gtaagtcaag ttcagtttta attgtttgac gattttgacg tgtaaccatt ttcgttggct
     8761 taactttttc tttttgtttt ttctctaaat cttcaatagg taacttcatt ttaataattc
     8821 ccatttgaac aattgcttct tcatcattga caatttctaa aacttcaccc ttttgaccgt
     8881 aagataatac ttttacttca tcaccagcaa caattttatc gtatttttgc ttttgtacat
     8941 tttgctttat agatttcgct tcataatgat catctaatcg tttcttctta tcaatcaatt
     9001 catgttcttt aacatctgca cctttttgtt cacgcaattg tcttaagtct ttaataatat
     9061 cgtcagcttc ttttgttgca gccttaatct tctgatttgc tttttctttc gcttcctcta
     9121 ttagagactt ttcataattt tggaattgtt gatactgctt agataaatca tcatgcactt
     9181 gctccgcttc ttttacaaga cggtccagtt ctaacctctg tgtctctaca cgtttgtaat
     9241 tacgctctaa tgattcaatc atttcattta tttctttttc atcagtacca atcatcgtct
     9301 tagccttatt aataatattc aaactaagac ctaacttttt agaaatgtca aaagcatttg
     9361 aacgacccgg cacacccatt aataacttat acgttggact caaagtatct acatcaaatt
     9421 ctacactcgc attcataacg ccttctcgat tataactata tgctttaagt tcaggatagt
     9481 gcgtcgttgc cattactaga gaaccaattt ttctaacatg atctaaaatg ctcattgcta
     9541 atgcagcacc ttcactcgga tctgtacctg cacctaattc atcaaataaa actaaactat
     9601 gtttgtctgc atgctttaaa atttcaacta tattcgtcat atgagatgaa aaagttgata
     9661 atgattgttc tattgattgt tcatctccga tatcgcaata tacattttta aatacactta
     9721 actgactacc atcaagtgtg ggaatcaaca atcctgattg agccataaca ataattaaac
     9781 ctaatgtttt taatgttaca gttttaccac ctgtattcgg tcctgtaata attaccgttt
     9841 caatatcttc cataaattcg atggtattag ctacaacagt ctcacgattt aataatggat
     9901 ggtatgcttt aggtaaatat acagtacggt cctctttaaa tatcggcttt gttcctttaa
     9961 tacttctact atatctcgct tttgcgatta aaaaatctaa ctgacccatg acttgttctg
    10021 ccacaagtag tgcatctttg tccgcagcca cataaccagt tagttgcgtt aaaatgcgtt
    10081 ctttttcaat tgcttcgtca tgacgtaatc gactaatttg attattcatt tcaacaactg
    10141 atgatggctc aatatacaat gtttgtcctg aagcagattg atcatgtaca atcccattaa
    10201 aatcttgtcg atattcagct ttgacaggta taacgtttct ttcattccta actgttacaa
    10261 tagcatctga taattttttc tgatttgctt ggcttttaac aatacggtcc aaattttgtc
    10321 taatacgttg attcgtgcta gaaattttac ttctaatccc ttgcaattca taactcgcat
    10381 tatcatataa atcatacgta tcgcatgttt catttatttg ttgaaaaaga tcagttaaca
    10441 caggtaattg attcatcttg tcatctaata ttgggtattt aacaccttca tcttcttcaa
    10501 ccaattgatt ataaaatgtc ttgaattgat tttgtacttg aattaatctt tttatcaagt
    10561 taagctctga tacatttaaa acgccgccaa tatcagcgcg atgaatgaat gctgatactt
    10621 tagataagcc actcaagctt ggtaaacgat gcttattata gatttgagca atctcatccg
    10681 tttcttccat ttgaaaaaca accgtttcaa aattagtagc tggcatcatt tgattgacct
    10741 tttccaagcc taagtcacta atagtttcat tggcaacgag tgattttatt ttttcaaatt
    10801 ctaagacgtc taatgttttt tgtctcataa aatccctcta tttcttaagt tttgtattat
    10861 tttcaataaa gtctttaaaa gcttcacgcg ataatgcgtt aatcactcta tcttttgtta
    10921 caaatccttt ttgcgcagtt gctacgccat aattcataaa atctaaatga tttgtatgat
    10981 gcgcatcagt gttaatagtt aatttcacat ttggatattt acgaacgata tcagcgctca
    11041 gatccagtcg atgtggattg gcattaattt ctaatactgt attcgtttct tcagctaatg
    11101 ccattaattg ttcaatattc ggtttataac catctcttct acctataata cgccctgttg
    11161 gatgcgctat atgtcgcacg tatggattgc gacatgcatt agctaatcgt tccataattt
    11221 gttcttctga ttggttaaag ctttgatgaa tagctccaat tacataatca agttgtgcta
    11281 aaatttcatc atcataatcc agcgagccat caggtaatat atccatttct gtacctgaat
    11341 aaatatcaat ttcactatat tctttatcta aagccttaat ttcttcgttt tgtcttaaaa
    11401 gtctttctac ttgtaagcca ttagcaacac gtaaactttg tgaatgatca gtaattacca
    11461 tgaatttata accttttgcg atatttgctt ctaccatgtc tcgaatagaa aacgcaccat
    11521 cactatacgt tgtatgcata tgaatatcac cattaatatc atctattgta atgatattac
    11581 ttagatcttt atcaaattcg ctaccatctt ctcgcatagc aggtggtata aaattcacat
    11641 taaaatgttc atatatcttg gcttcactat catattgaat taatgtacca tcagcttgtt
    11701 caattccata ttcacttact ttttcatcac gtgctttagc aagttgtcga attcttatat
    11761 tatgttcttt tgacccagta aaatgctgca atgtatgata aaaagcactt ggttcaatta
    11821 atcgaaaatc gacaccaatc gtttcatcat catacgctaa ttctaatgaa acttttgtgt
    11881 tccccactgc aacttctttt actttattgg gaatatttaa taattgctgc tgcactgctt
    11941 ttgggttatc ggtacttatt atgaaatcta aatctttgct catttcttta aaacgacgga
    12001 agcttcctgc agatgaatat tgatcgatat aatttaatgt atctatataa tcaatgattt
    12061 cttgattaag tcttctcatt tgatcaattg gatatctatc tttcttagca ccaagttgtt
    12121 tcacagcttc taatatgttt tgttccgttt tcttagcaaa tccgcttaat tcactaactt
    12181 ttccattttc acaagcaact tgaagtgacg ctttatcaac aatattcaac tctttatata
    12241 gcttagcaat tttcttgctt ccaagtcctt gaattttcaa aagtggaata agaccttccg
    12301 gaacttcttc ctgtaattgc tgtaaatact gagattcacc ggtctcacgg taatcattga
    12361 ttacttctgc aacaccttta ccaatgcctt ttaactccgt tacatcagat atttcatcta
    12421 atggtcgttc atctaattca agactttgag ctgcttttcg atacgctgat attttaaaag
    12481 tattttcccc ttttaattcc atataagtag caatttgttc taatagtttg ataacatctt
    12541 tttttgtcat aataacactc cataaaaaga agaccaggac gtatcattaa tatatacctt
    12601 tgtcctgacc tcttatgata atttataaat taatggtgaa agccgataaa tatgggatgt
    12661 gaaaaatcaa tgaatgactc acaatagata ttttaagctg atgttgtaat gcttcgttag
    12721 gatataatgc caatagatat aacgtaaagt gtaagacaat tatcgtcata aacacaccaa
    12781 ctatcattcc cattgcacga ctgaaaatat gaatgttttg atacgctatt attttatcaa
    12841 aagttacgat aattagatat aaaatgaact tacaaaacaa tgtaatcatt aaaaaagcta
    12901 caatcgcttc aaatcgattt tgtagatgat taaaatgaaa cgcaaaagtt gtattaaatg
    12961 ctgttgtttt aggatatgga ataaatacaa ttaatctttc tacaatagat ttgtaaaatt
    13021 gactggcaat ccacaatgat acaatcgttg cactcaaatg tatcatagat aaccaaaaac
    13081 ctcgtctgaa tccaacgatg acaaaataca caaagaaaat gattatgata aaatcaatga
    13141 ccatttattg ctcacgctgc tgcaatttgt gaatttgttg tttcaaacgt cgattttctt
    13201 cttctagtag tactttttca tgcataatat tcacagcagt tagtattgct tttcttgaag
    13261 tatctaaacc tgctgcttta taccctaatt cttttatttt atcatcaact aaatgtgcta
    13321 catatcgtat gtgctctggg ttatcttccc caacaattgt aaaaagctga tcattaattg
    13381 atacatttac cttgttttta aactgtgtca tttataattt ctcctgatcc tttttttaaa
    13441 atctaaattc acgttataaa atatgactgg atagtttgt
//
LOCUS       DAKTLH010000069        13073 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.069, whole genome shotgun sequence.
ACCESSION   DAKTLH010000069 DAKTLH010000000
VERSION     DAKTLH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13073
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.069"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..187
                     /gene="mreD"
                     /locus_tag="O0394_001616"
     CDS             <1..187
                     /gene="mreD"
                     /locus_tag="O0394_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCY0499696.1"
                     /translation="VYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLII
                     KFLKKTNNDIDMKRRQW"
     misc_feature    214..341
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            354..662
                     /gene="rplU"
                     /locus_tag="O0394_001617"
     CDS             354..662
                     /gene="rplU"
                     /locus_tag="O0394_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCY0499697.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            668..988
                     /locus_tag="O0394_001618"
     CDS             668..988
                     /locus_tag="O0394_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCY0499698.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            1000..1284
                     /gene="rpmA"
                     /locus_tag="O0394_001619"
     CDS             1000..1284
                     /gene="rpmA"
                     /locus_tag="O0394_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCY0499699.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
     gene            1639..2931
                     /gene="obgE"
                     /locus_tag="O0394_001620"
     CDS             1639..2931
                     /gene="obgE"
                     /locus_tag="O0394_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCY0499700.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            2944..3399
                     /locus_tag="O0394_001621"
     CDS             2944..3399
                     /locus_tag="O0394_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCY0499701.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            3413..4015
                     /gene="ruvA"
                     /locus_tag="O0394_001622"
     CDS             3413..4015
                     /gene="ruvA"
                     /locus_tag="O0394_001622"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCY0499702.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            4047..5051
                     /gene="ruvB"
                     /locus_tag="O0394_001623"
     CDS             4047..5051
                     /gene="ruvB"
                     /locus_tag="O0394_001623"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCY0499703.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERE
                     "
     gene            5053..6078
                     /gene="queA"
                     /locus_tag="O0394_001624"
     CDS             5053..6078
                     /gene="queA"
                     /locus_tag="O0394_001624"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCY0499704.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLIEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSSRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            6101..7240
                     /gene="tgt"
                     /locus_tag="O0394_001625"
     CDS             6101..7240
                     /gene="tgt"
                     /locus_tag="O0394_001625"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCY0499705.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            7259..7519
                     /gene="yajC"
                     /locus_tag="O0394_001626"
     CDS             7259..7519
                     /gene="yajC"
                     /locus_tag="O0394_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCY0499706.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            7794..10073
                     /gene="secDF"
                     /locus_tag="O0394_001627"
     CDS             7794..10073
                     /gene="secDF"
                     /locus_tag="O0394_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HCY0499707.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
     gene            10276..12549
                     /gene="recJ"
                     /locus_tag="O0394_001628"
     CDS             10276..12549
                     /gene="recJ"
                     /locus_tag="O0394_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCY0499708.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTATTILVIT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDVEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     QVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            12571..>13073
                     /locus_tag="O0394_001629"
     CDS             12571..>13073
                     /locus_tag="O0394_001629"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCY0499709.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSL"
BASE COUNT         4678 a         1803 c         2559 g         4033 t
ORIGIN      
        1 agtctatgtg gcattaatat acggtatgtt aggattcatt caatttgata ttattcattt
       61 tgtagtcttt agattattgc caacattaat tatgaatttt gtactgttga ttatgcttta
      121 tccgttgatt ataaagttcc ttaaaaaaac aaacaatgac attgacatga aacgtcgtca
      181 atggtaagat gcaatagttg agtagttata agtagctaca tacaaccgct caaatatagg
      241 ttcaagaaca tttttaatga agaaagcgat tttcccacag agtgcaaacg cttgactcaa
      301 taacgaaatg tcacctatgt atggcgtgac ttattataca ggaggtgcaa agtatgtttg
      361 ctattattga aacaggtgga aaacaaatca aagtagaaga aggtcaagaa atcttcgttg
      421 aaaaattaga cgtaaacgaa ggagatactt ttacatttga taaagtatta tttgtaggtg
      481 gagattcagt taaagttgga gcgccaacag ttgaaggtgc aacagttact gctactgtta
      541 ataaacaagg tcgcggtaaa aaaatcactg tattcacata caaacgtcgt aaaaattcaa
      601 aacgtaaaaa aggccatcgt caaccataca ctaaattaac aatcgataaa atcaacgcgt
      661 aattattatg attactgttg atattacagt taatgatgaa ggcaaagtaa cagacgttat
      721 tatggatggc catgctgacc atggtgaata tggtcatgat atcgtttgtg ctggagcttc
      781 agctgtattg tttggtagtg ttaatgcgat tataggattg acatctgaga gaccagatat
      841 caattatgac gacaatggtg gtcattttca tataagaagc gttgatacaa acaacgatga
      901 agcgcaacta attcttcaaa caatgcttgt gtctttacaa actattgaag aagaatataa
      961 tgagaatatt agattaaatt ataagtgagg tgcattccga tgttaaaatt aaacttacaa
     1021 ttcttcgcat ctaaaaaagg ggtaagttct acaaaaaacg gacgtgactc tgaatcaaaa
     1081 cgcttaggtg ctaaacgtgc tgacggtcaa ttcgtaacag gtggttcaat tttatatcgc
     1141 caacgtggta ctaaaattta ccctggtgaa aatgtaggtc gtggtggcga tgatacatta
     1201 ttcgctaaaa tcgacggcgt tgttaaattc gaacgtaaag gtcgcgacaa aaaacaagtt
     1261 tctgtatatg cagtagctga ataattttgt ctagttaaca ccagaagtga atcttctggt
     1321 gttttttact ttttataaaa tgtttttcat tattattttc attatgttat taaaaaatgg
     1381 gaattttaga cgttatacta aatgtgcaac tgtataagag cccctaatca ctaaactata
     1441 agggggacaa aggaatacag ttgcagcgtt taaagaataa actgtaccac aattggtgct
     1501 gagaaatata agtattttaa agcaaagatt tataaaagta actgcataag agcccctaat
     1561 tatttacaat atataagggg ctctaatatg ctataattat tgggaaaatg aaaattatat
     1621 gtaaaagagg tgagatatat gtttgtcgat caagtcaaaa tatctcttaa agccggtgat
     1681 ggtggtaatg gtattaccgc atacagaaga gaaaaatatg taccatttgg tggaccagct
     1741 ggcggtgacg gtggtaaagg tgcttcagtc gtatttgaag tggatgaagg tttaagaacg
     1801 ttattagatt ttagatatca acgtcatttt aaagcaagca aaggtgaaaa tggccaaagt
     1861 agtaatatgc atggtaaaaa tgcggaagat ttagtattaa aagttccacc tggtacaatt
     1921 attaaaaatg ttgaaacaga cgaagtgtta gcagatcttg ttgaagatgg tcaaagagct
     1981 gtagtagcga agggcggtcg aggtggccga ggtaattcac gttttgcaac acctagaaac
     2041 cctgcacctg acttcagtga aaaaggtgaa ccaggtgagg aattagatgt atctttagaa
     2101 ttgaaattat tagctgatgt aggattagta ggtttcccta gtgtgggtaa atcgacttta
     2161 ttatctatcg tttcaaaagc taagcctaaa attggggcat atcattttac aacgattaaa
     2221 ccaaatctag gtgttgtttc aacgcctgat caacgtagtt ttgttatggc agatttacca
     2281 ggtttaattg aaggtgcatc tgatggcgtt ggattaggac atcaattttt aagacatgta
     2341 gagagaacaa aagttattgt tcacatgatt gatatgagcg gttctgaagg tagagaacct
     2401 attgaagatt ataaagtcat taatcaagaa ttagctgcgt acgagcaacg tttagaagat
     2461 agacctcaaa tcgtagtagc taacaagatg gatttacctg aatcacaaga taatttaaac
     2521 ttgtttaaag aagaaattgg cgaagatgtg ccagttattc cagtttcaac aataacgcgt
     2581 gataatattg atcaattatt atatgcaata gcagataaat tagaagaata taaagatgtt
     2641 gacttcacag ttgaagaaga ggagtcagtt ggcattaacc gagtattata taaacataca
     2701 ccgtcacaag ataaatttac aatttcaaga gatgatgatg gtgcttatgt ggtaagtggt
     2761 aatgctattg aaagaatgtt taaaatgact gactttaaca gtgatccagc agtacgtcga
     2821 tttgctcgtc aaatgcgttc gatgggtatt gatgatgcgc ttagagaacg tggttgtaaa
     2881 aatggtgata tcgttagaat tcttggcgga gaatttgaat tcgttgaata ggagcgaaac
     2941 atgatggaca ataaagatta taaaaagttt tatttaatta gagaagatgt cttgcctgaa
     3001 tccgtggtta aaacattgaa gattaaagat gccttaaaaa gtgatccgac attgtccatt
     3061 tatgatgccg ttaaacagtt tgatctatct agaagtgctt tttataaata tagagaaacg
     3121 atatttccag tagacgataa aatgcttgac catcgagaat ttacattaat tttatatgta
     3181 actgatattg ttggtatgtt ggcacgtgta ctagatgtta tatcaaagtt agaactatct
     3241 gtattaacga ttcatcaaag tattccaatg gaagaaaaag caacaataac attatcactg
     3301 aatgctaaat ctaaagaaac ttcagtagaa gatgttattg gcgctttgag aaatttagat
     3361 tatgtatcaa aagtagaatt aattagtatg agtatgtaag gatgtgccta taatgtacgc
     3421 gtatgtcaaa ggtaagttaa cacatttata tcctacacac gtagttgttg aaactgctgg
     3481 tgttggttat gaaattcaaa caccaaattc ttatcgtttt caaaagcatc tagatcatga
     3541 agttttaatt catacatctt taattgttcg tgaagatgca caattattgt atggatttag
     3601 tagtgaagaa gagaaagata tgttcttgag tttaattaaa gttactggta ttggtccgaa
     3661 atcagcttta gctattttag cgacaagtac gcctaatgaa gtaaaacgtg ccattgaaaa
     3721 tgaaaatgat acgtatttaa ctaaattccc aggaattggt aagaaaacgg caagacagat
     3781 tgtcttagat ttaaaaggta aagtgaaaat tactgaagaa gatagcgatt cattattaca
     3841 agtagacgct acttcgacgg tgcaagatca attcgtgcaa gaagcaatgt tagcgttaga
     3901 agcattaggt tattctaaac gagagcttgc aaaagttgag aaaacgttaa ataaaaataa
     3961 atatgactca gttgatgaag ctgttaaggc aggtcttcaa ttagttgtat cttaatttta
     4021 aatagattaa taggggaagt gttgtcatga atgagcgtat ggttgatcaa tcaatgcata
     4081 gtgaagaaac tgatttcgaa ttgtcgctta gacctacgag attacgacaa tatattggtc
     4141 aaaattcaat aaaaagtaat ttagaagtat ttattaaagc ggctaaactt cgtcatgaac
     4201 cattagatca tgtattgctt tttggccccc ctggattagg taagacaaca ttatctaata
     4261 tcattgccaa tgaaatggaa gttaatatac gtacagtatc agggccttca ttagaaagac
     4321 ctggtgattt ggctgcaatt ttatcaggac ttcaacctgg agatgttttg tttattgatg
     4381 aaatacacag actgagtagt gttgttgaag aagtgttata ccctgcaatg gaagatttct
     4441 ttttagatat tatcattggt aaaggcgatg aggctagaag tatccgtatc gacttacctc
     4501 cattcacttt ggtaggtgca acaacgcgag ctggcagctt aacaggtcca ctaagggatc
     4561 gatttggtgt gcacttaaga ttagaatatt ataacgaatc agatttaaaa gaaatcatta
     4621 ttagaacagc tgaggtttta ggcacaggta ttgatgaaga aagtgccatt gaacttgcta
     4681 aacgttctag agggactcca agagtagcaa atcgactatt gaagcgggta agagacttcc
     4741 agcaagtgaa tgaagatgaa caaatataca ttgaaacaac gaagcacgca ttaggtttac
     4801 ttcaagttga tcaacacgga ctagattaca ttgatcataa aatgatgaac tgtattatta
     4861 agcagtataa tggcggacct gttggtttag atacgattgc cgtaacaatt ggtgaagaac
     4921 gtattacaat tgaggacgtt tatgagccat ttcttattca gaaaggcttt ttagaacgta
     4981 cgccacgtgg cagaaaagca acaccattag cttatgaaca ttttgcaaag tcgaatgagg
     5041 agagagaata acgtgaatat tgaagaattt gactatgact taccagaatc attaattgct
     5101 caaacgcctt taaaagatcg tgatcatagt cgtttattag tcatggatag agaaactggt
     5161 gaaatgaaac atttacattt caaagatatc attgagtatt ttagacctgg tgatacatta
     5221 gtgcttaacg atacgcgagt aatgccagct agactttttg gtttaaaaga agaaactggt
     5281 gcaaaagttg aaatgttaat gttaactcaa attgaaggta atgattggga agtcttactg
     5341 aaaccagcta agcgtattaa agttggtaat aaattgaatt ttggtaatgg caaaattata
     5401 gctgaatgca taaaagaaat ggatcaaggt ggacgcatca tgcgtttaca ttatgaaggt
     5461 attttacaag aaagattaga tgaattaggg gaaatgccac tgccaccata catcaaagaa
     5521 cgtttagatg atccagatcg ttatcaaaca gtttacgcta aagaaagtgg ttcagcggca
     5581 gcaccaacag caggattaca ttttactgat gagttattaa ttgaaattaa aaataaaggt
     5641 gttaatatcg catttgttac attacatgtt gggttaggta cgtttagacc ggtgagcgta
     5701 gacgatgtga atgaccacga aatgcatagt gaatattatc aaatgacaca agaaacagct
     5761 gatttattaa atgatactaa gtccaaagga catcgcatta tatcagttgg tacaacttca
     5821 acacgtacac ttgaaacaat tcgacgcgat catgataaat ttgttgaaac gagtggctgg
     5881 actaatatat ttatttatcc aggatttgat tttaaagcaa ttgatggcca gattactaat
     5941 tttcatttac caaaatcaac attagttatg ctagtatcag cgtttagtag tcgtgaaaat
     6001 gttctgaatg cttataaaac ggcagtaaat ttagaatata gattctttag ttttggcgat
     6061 gcaatgttaa ttatataaaa agaatgtgag gattttgaat atgcctgcag taacatacga
     6121 acacattaaa acttgtaaac aatcaggtgc gcgtttaggt atcgtgcaca caccacacgg
     6181 ttcatttgaa acacctatgt ttatgccagt tggtactaaa gcaaccgtta aaacaatgag
     6241 tccagaagag ttaagacaaa ttgaagcaaa aatcattttg ggcaacacat atcatttgtg
     6301 gttacaaccc ggaaatgata ttatcaaaca cgctggggga ttacataaat tcatgaattg
     6361 ggatggtccg attcttacag attcaggcgg tttccaagtg tttagtttaa gtaatttacg
     6421 taaaattaca gaagaaggcg tggaatttag acatcatact aatgggtcta aattattttt
     6481 gagtcctgag aaatcaatgc aaattcaaaa tgatttagga tctgatatta tgatggcatt
     6541 tgatgaatgt ccaccgatgc ctgctgaata tgattatgta aaaaaatcta ttgaacgtac
     6601 aacacgttgg gcgaaaagat gtctagatgc acaccaaaga cctgaagatc aagcattgtt
     6661 cggcattata caaggtggcg aatatgaaga tttaagagaa caaagtgcaa aggatttagt
     6721 agaattagat tttcctggtt atgcaatcgg cggtttgtca gttggtgaac ctaaaccggt
     6781 tatgtataaa atggttgaac atacagagca gtttatgcct aaagataaac caagatattt
     6841 aatgggtgta ggatctccag atgcgttaat cgaatgtagt attcgcggca tggatatgtt
     6901 tgattgtgtc ttaccgacac gtattgccag aaatggtact tgtatgacat cgcaaggtcg
     6961 tttagttatt aaaaatgcaa aatttgcaga tgatttaaga ccgttagatg agaattgtga
     7021 ctgttataca tgtcaaaact attcaagagc gtatatacgt catttaatca aggcagagga
     7081 aacttttggt attcgtctta ctactattca taatttacat tttctgctaa aattaatgga
     7141 agatataaga caagccattc gagaagatcg tcttttagat ttcaaagaag aattcttcga
     7201 gcaatatgga ttaaatgttg agaacccaaa aaacttttaa gcaagaggag cgtataaaat
     7261 gcaattttca ttactaatat atatagtcgt aatttttgcg gttatgtatt tcttgatgat
     7321 cagaccacaa caaaaacgtg cgaaacagca tcgtgagttg attaataaca ttcaatctgg
     7381 tcaaagaatt acaactattg gtggtattaa aggtactgtt aaagcagtag atgaaacaac
     7441 tgttgttatt acagttaatg gtcatggtac tgaattaact ttcgaaaaac ctgctattaa
     7501 acaagttgac ccttcataat gagaagtggt attaaaaagg atgaaacgaa tagcgtttca
     7561 tcctttttaa gttataagta gtatgtagaa tagcttattt ttattccaaa aaaagaaatt
     7621 gtgatacata ataattgtta aatttaaatt aaatatatct atgactattt ttactccctg
     7681 ctctagtatg ttaatatgta taaggctaat aaattttgta agaaagattt aacttgcgtt
     7741 ataaaatagt attcaagtat tctaaataca tacgtaaata atgaggtgtt catgtgaaga
     7801 aaagtagtag aataattgcg ttcttgcttt tagtcgtatt gttgtttgcc ggaatggctg
     7861 caacttataa aagtgttata aagaatgtca atttaggact tgatttgcaa ggtggatttg
     7921 aggtgcttta tcaagtcgat cctttaaata aaggcgacaa aatagataaa aaagccttac
     7981 aatcaacagc gcaaacatta gaaaatcgtg ttaacgtttt aggtgtttcg gaacctaaaa
     8041 ttcaagttga agaacctaat agaattagag tacaacttgc tggtgtaaca gaccagaatg
     8101 aagcacgtaa aattttatct tctcaagcta atttaacaat tcgcgatgca gaagataaag
     8161 taaaattaag cggttctgat attaaacagg gatctgccaa acaagaattt aaacaggaaa
     8221 caaaccagcc tacagttact tttaaagtaa aagacaaaaa caaatttaaa aaagtaacgg
     8281 aagaaatctc taagaaacgt gataatgtca tggttgtttg gttagatttc aaaaaaggtg
     8341 acagctataa aaaagaagca caaaagaaaa accctaaatt tatttctgca gcaagtgttg
     8401 atcaaccaat caattctgat agcgtagaaa tctcgggtgg atttaaagga caagaaggcg
     8461 ttaaaaaagc aaaacaaatc gccgaattgt taaatgcagg ttctttacca gttgacttga
     8521 aagaaattta ttcaaactca gtaggtgccc aatttggtca agatgcatta gataaaactg
     8581 tgtttgcatc gtttattggt gtagcattaa tttatttatt catgcttgga ttctaccgat
     8641 tacctggttt agtagcgatt attgcattga caacttatat ctatctaacg ttagtagcat
     8701 ttaatttcat ttccggggtt ctaactttac caggattagc ggcgttggta ttaggtgtag
     8761 gtatggctgt agatgccaat attatcatgt atgagcgtat taaggatgaa cttcgaatag
     8821 gtagaacgat aaagcaagcc ttttctaaag caaacaaaag ttcattccta acaatttttg
     8881 attctaactt aacaacagtt atcgccgcag cagtattatt cttcttcggt gaaagttcag
     8941 ttaaaggttt cgcgacaatg ttattattag gtattctaat gatctttgtt acagccgtgt
     9001 tcttatcaag attcttatta tcattacttg tttcatcaaa tatattcaaa aatcaatttt
     9061 ggttatttgg tgttaaaaag aataaacgac atgatattaa tgaaggtgta gatgttcatg
     9121 accttaaaac ttcattcgag aaatggaatt ttgttaaatt agctaagcca ttaattggag
     9181 taagtatttt aattgtagtt gtcggtttag ttattcttta tatcttcaag ttaaacttag
     9241 gaattgattt ctcaagtggt acacgcgtag atttccaatc taaacaagca attactcaac
     9301 aaaaggtaga gcaagttgta aaagatagtg gacttaaagc ggatcaaatt cagatcaatg
     9361 gtaaagacaa taaagtagca actgttcaat ttaaagacga tttaacgcgt gctcaagata
     9421 ataaattgag tgataatatt aaatcgaaat tcggtgacac acctcaaatt aacactgtat
     9481 caccaattat tggacaggaa ttagcaaaaa atgcaatgct tgcattaatc tatgcatcga
     9541 taggtatcat catctatgta tcattacgat ttgaatggcg catgggtctt tcatctgtat
     9601 tggcattatt acatgatgta ttcattatag tagcgatttt cagtttattt agaattgaag
     9661 tagatttaac atttatcgcc gctgtattaa caattgtcgg ttattcaatt aatgatacaa
     9721 tcgtaacgtt tgaccgtgta cgtgaaaact tacaaaaggt taaagtgatt acgacaacag
     9781 aacaaattga tgatatcgtt aatagatcaa ttagacagac aatgacacgt tcaattaata
     9841 cagtattaac agttattgta gtagtagttg ctatactatt cttcggtgct cctacgatat
     9901 tcaactttac tttagcatta tttatcggat tgatttctgg tgtattctct tcaatcttca
     9961 ttgccgttcc gctatgggga ataatgaaaa aacgtcagtt gaaaaaatcg ccgaaacaca
    10021 aattagttgt atataaagaa aagaaatcga acgatgaaaa gattttagtt taaaatgaat
    10081 taagcggtat gtgaaacaat aaagagttca cataccgctt tttactttat ttaaatttaa
    10141 caaaatatat ataaataagt tcccgcattc atttttaaaa aatggtataa ttaaagtctt
    10201 gattatttag ataagtgctt tttatttaaa gtaattttag atataatgtc ttttggaaat
    10261 gaggcgtaaa tatatatgat taaaccgaag tataagtgga aattaactaa accagctgaa
    10321 tacataagtg atgaattaac atcaaaatta aagttaacgc caatcgtaaa aaaaatatta
    10381 gaaagtaaat cgattattga tgaacaagca atagaatcca tcatttcaga tacagatatc
    10441 aatcatgatg cactgcaatt gagcgatatg actaagacta ttgaaagaat taaaagagcg
    10501 atagcgaatg atgagaaaat cctagtttat ggtgattacg atgccgacgg tgttaccgca
    10561 actacaattt tagtgataac attacaactt ttaggtgcac aagtaggatg gcatattcct
    10621 aaccgcttta ccgaaggata tggaccaaac gaactagcat ttagaaatgc tcatgatgaa
    10681 ggcataacat taatcataac tgttgataat ggcatacaag gtcataatga aattaaaatg
    10741 gttcaagatt taggagttga tgtcattgta actgatcatc atgaaattgg tagtacatta
    10801 ccagaagcat atgcaatcgt acatccaatg catccatcgt ttaattatcc gttccaacaa
    10861 ttgtgtggtg caggtgttgc atacaaatta gcacaagcat tgattgaaaa tgtacctgat
    10921 tattttaaag cattagttgc gataggtacc attgcggatt tagtatcctt aactgatgaa
    10981 aatcgatcat tagtgaaaca aggattaaaa gtcttaaatg atcaatgtcc gacctctgtt
    11041 aaagcattgc taaaagaagc gggatacaat gacaatattg atgaagaaac aattggtttt
    11101 attattggac ctagacttaa tgctgtaggt cgtttagatg acgcatcatt agcttgcgaa
    11161 ttgttgatga ctgacgtcga agaagaagct gcatttttag cagaacaagt tgaacatttt
    11221 aaccgtgaaa gaaaagatat tgtagcaact atcacagaag aagcaatggc tatggcagaa
    11281 acgaaagtta aaaagggaga tttattttta cttttagcaa aagaaaattg gcatgaaggt
    11341 gtcttaggta ttgttgcttc gaaaattgtc gaaacatttg ctttgccaac attaatttta
    11401 aatattgata gggagcaaaa tcatgcaaaa ggctccgcca gatcaattga tcaagtgtcg
    11461 atgtttgaaa tactatcagc acatcaagag ttaatcgcta aatttggcgg ccatcatatg
    11521 gctgcaggta tgacgatgga tattgaaaat atagaatcgt tagctgaagg tttaaataaa
    11581 tggatgaaag aattatctaa aacaacttcg ttagatcccg taaagcaagt agatgtatta
    11641 ttaacagaaa atgatataac gattaaaaat attcgtgata tgaatcgttt aagacccttc
    11701 ggaacagatt tttctagacc tatttttgaa atggatgatt tatcagtttc ttcagttaag
    11761 gcgataggtc aacaaaaaaa tcatctaaaa ttgacacttg gtgaaagtaa tatcgccgcg
    11821 ctattttggc aaaatggaca cttagaacct gagttacaag atgaacagcc aattaatata
    11881 ttaggatctg ttcaaatcaa cgaatggaat ggtaatcaat caccgcaaat aattattcaa
    11941 gatattgcga tgaatgaaca gcaaatatta gattatagaa gtaagcgaaa aagtttacct
    12001 tttacagaaa atgatgaaaa tattgtcgtg cttattcatc ctaaaagtga taaagtaaat
    12061 gcgaatgaat attattatgg tgaagaaatt aaacaacaaa ctgataaagt agtattaaga
    12121 gatttaccaa cgtcaatgga agacttgtct aattccttgc aacaactgca attttctcaa
    12181 ctttatatag ttttgcaaca taatcattcg atttacttcg atggtatacc taatatggat
    12241 atttttaaaa agtgttataa agcattaata actaaacaag aaacaaatat ccagaaagag
    12301 ggtatgttat tgtgtcaaca tttaagtgtg aaaccagata cacttaaatt catgttgaaa
    12361 gttttcttag acttaaaatt tgtaacacaa gaagatggtt taattcgaat caatcaacaa
    12421 cctgataaaa gatcgattga ttccagcaaa gtatatcaat taagacaaca acgtatggat
    12481 gttgaaaagc aattattata tcaagatttt tcagaaataa aaaattggat aaagtcacaa
    12541 ttgtcgtgag caatttagga ggaaatatta atggatttaa agcaatacgt atcagaagtt
    12601 caagattggc cgaaaccagg tgttagtttc aaggatatta ctacaattat ggataatggt
    12661 gaagcatatg gctatgcaac agataaaatt gtagaatacg caaaagacag agatgttgat
    12721 atcgttgtag gacctgaagc gcgtggcttt atcattggct gtcctgtagc ttattcaatg
    12781 gggattggct ttgcacctgt tagaaaagaa gggaaattac ctcgtgaagt cattcgttat
    12841 gagtatgacc tagaatatgg tacaaatgtt ttaacaatgc acaaagatgc aattaaacca
    12901 ggtcaacgtg tgttaattac agatgattta ttagctactg gtggtacgat tgaagcagca
    12961 ataaaattag ttgaaaaatt aggcggtatc gtagtaggta ttgcatttat aattgaattg
    13021 aaatatttaa atggtattga aaaaattaaa gattacgatg ttatgagttt aat
//
LOCUS       DAKTLH010000070        12660 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.070, whole genome shotgun sequence.
ACCESSION   DAKTLH010000070 DAKTLH010000000
VERSION     DAKTLH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12660
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.070"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            1..729
                     /locus_tag="O0394_001630"
     CDS             1..729
                     /locus_tag="O0394_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0499710.1"
                     /translation="MFLVISITAGCGIGKEAEVKKSFEKTLSMYPIKNLEDLYDKEGY
                     RDDQFDKNDKGTWIINSEMVIQPNNEDMVAKGMVLYMNRNTKTTNGYYYVDVTKDEDE
                     GKPHDNEKRYPVKMVDNKIIPTKEIKDEKIKKEIENFKFFVQYGDFKNLKNYKDGDIS
                     YNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTSKAPKLLLKGSGNLKGSSVGYKDIEF
                     TFVEKKEENIYFSDSLDYKKSGDV"
     gene            781..1551
                     /locus_tag="O0394_001631"
     CDS             781..1551
                     /locus_tag="O0394_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0499711.1"
                     /translation="MIKRVNKLVLGISLLFLVISITAGCGMGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVNSQMAIQNKGEALKIKGMLLKIDRNTRSA
                     KGFYYTNEIKTEKYEVAQDNQKKYPVKMINNKFISTEEVKEENIKKEIENFKFFAQYS
                     NFKDLMNYKDGDISYNPEVPSYSAQYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKKIEFTFLENKNENIYFTDSLHLEPSEDK"
     gene            1615..2385
                     /locus_tag="O0394_001632"
     CDS             1615..2385
                     /locus_tag="O0394_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0499712.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAKIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMATQNKGEALKVKGMVLYMNRNTKTT
                     KGYYYVNAIKNDKDGRPQENEKRYPVKMVDNKIIPTKEIKDKNIKKEIENFKFFVQYG
                     NFKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKDIEFTFVEKKEENIYFSDGLIFKPSEDK"
     gene            2452..3219
                     /locus_tag="O0394_001633"
     CDS             2452..3219
                     /locus_tag="O0394_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0499713.1"
                     /translation="MKRLNKLVLYISFLILVISFTAGCGIGKEAEVKKSFEKTLSMYP
                     IKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMVVQPKGERMKSKGMVLYMNRNTKTTT
                     GKYIVSETLHDEDGRPKSKDKEYPVKMVDNKIIPTKGIKDENIKKEIENFKFFAQYGS
                     FKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYKIPTNKAPKLLLKGSGD
                     LKGSSVGYKDIEFTFVEKKGENTFFTDSLHLEPSEDK"
     gene            3313..5550
                     /locus_tag="O0394_001634"
     CDS             3313..5550
                     /locus_tag="O0394_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0499714.1"
                     /translation="MQRDYLIRVETESMSDFKRLNSLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDVHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFHHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHDYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFQPTNHQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFQVNEVLAYDVIPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLDQYDSRDYIDAFKIFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHRLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCKNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEEGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            5701..6879
                     /locus_tag="O0394_001635"
     CDS             5701..6879
                     /locus_tag="O0394_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0499715.1"
                     /translation="MNQQLIETLKSKEGKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKTMIENGVLDGVDHV
                     LGVHVMSTMKTGKVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEDMYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEMCEPQPPSED
                     FAYYAKERPSAFIYTGAAVENGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            6881..8269
                     /locus_tag="O0394_001636"
     CDS             6881..8269
                     /locus_tag="O0394_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0499716.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGTDM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRVIQGVSAACIMPSTLAIMKTYYQGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEITNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLNVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(8762..10423)
                     /locus_tag="O0394_001637"
     CDS             complement(8762..10423)
                     /locus_tag="O0394_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCY0499717.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDLWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(10749..12524)
                     /locus_tag="O0394_001638"
     CDS             complement(10749..12524)
                     /locus_tag="O0394_001638"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCY0499718.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHISGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKIDVTTSQKIAREILIDRNGNAE
                     SIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFT
                     INRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSPFDLRVLMDAIYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIESLLKEHKLL"
BASE COUNT         4438 a         1914 c         2309 g         3999 t
ORIGIN      
        1 ctgtttttag tcattagtat cactgctggt tgtggcatag gtaaagaagc ggaagttaag
       61 aaaagctttg aaaaaacatt gagtatgtac cctattaaaa atctagagga tttatacgat
      121 aaggaaggct atcgtgatga tcagtttgat aaaaatgata aaggtacatg gattataaat
      181 tctgaaatgg ttattcaacc taataatgaa gatatggtag ctaaaggcat ggttctatat
      241 atgaatagaa ataccaaaac aacaaatggt tactactatg tcgatgtgac taaggacgag
      301 gatgaaggaa aaccgcacga caatgaaaaa agatatccgg ttaaaatggt cgataataaa
      361 atcattccaa caaaagaaat taaagatgaa aaaataaaaa aagaaatcga aaactttaag
      421 ttctttgttc aatatggcga ctttaaaaat ttgaaaaatt ataaagacgg agatatttca
      481 tataatccag aggtgccgag ttattcggct aaatatcaat taactaatga tgattataat
      541 gtaaaacaat tacgcaaaag atatgatata ccgacgagta aagctccaaa gttattgtta
      601 aaaggttcag ggaatttaaa aggctcatca gttggatata aagatattga atttacgttt
      661 gtagagaaaa aagaagaaaa tatatacttt agtgatagct tagattataa aaaaagcgga
      721 gatgtataat catggctcaa tcagaatatg aaatcaatcc cggaaaaaga gagtgatgaa
      781 atgataaaac gtgtaaataa attagtgctt ggtattagtc ttctgttttt agtcattagt
      841 atcactgctg gttgtggcat gggtaaagaa gcggaaataa agaaaagttt tgaaaaaaca
      901 ttgagtatgt atccgattaa aaatctagag gatttatacg ataaagaagg atatcgtgat
      961 gatcaatttg ataaaaatga taagggaaca tggattgtaa attctcaaat ggcaattcaa
     1021 aataaaggag aagctctaaa aataaaaggc atgcttttga agatagatag aaatacaaga
     1081 agtgcaaaag gattttacta tactaatgaa ataaagacgg agaaatacga agtagctcag
     1141 gataatcaaa aaaaatatcc agttaaaatg attaataata aattcatttc tactgaggaa
     1201 gttaaagaag aaaacataaa aaaagaaatc gaaaacttta agttttttgc gcaatatagc
     1261 aattttaaag atttaatgaa ttataaagat ggagatatat catataatcc agaggtgccg
     1321 agttattcag ctcaatatca attaactaat gatgattata atgtaaaaca attacgtaaa
     1381 agatatgaca taccaacaaa taaagcgccg aagctgttgt tgaaaggtac agggaattta
     1441 aaaggttcat cagttggata taaaaaaatt gaatttactt ttttagagaa taaaaatgaa
     1501 aatatttact ttactgatag tctacatctt gaaccgagcg aggataaata atcatctttt
     1561 aaccagaata tgaaatcaat ctaggacaac ctaataaaag gagagagttg taagatgatg
     1621 aaacgattaa acaaattagt gttaggcatt atttttctgt ttttagtcat tagtatcact
     1681 gctggttgtg gcataggtaa agaagcgaaa ataaagaaaa gttttgagaa gacattgagt
     1741 atgtatccga ttaaaaatct agaggattta tacgataaag aaggttatcg tgatgacgaa
     1801 tttgataaaa atgataaagg tacatggatt attggttctg aaatggcaac tcaaaataag
     1861 ggggaagctc tgaaagttaa aggtatggtc ttgtatatga atagaaatac caaaacaaca
     1921 aaaggatatt attatgtaaa tgcaataaag aatgataaag acggaagacc ccaagagaat
     1981 gaaaaaaggt acccagttaa aatggtcgat aataaaatca ttccaacaaa agaaattaaa
     2041 gataaaaaca taaaaaaaga aatcgaaaac tttaagttct ttgttcaata tggaaacttt
     2101 aaagatttgt cgaagtataa agacggagat atttcatata atccagaggt gccgagttat
     2161 tcggctaaat atcaattaac taatgatgat tacaatgtaa agcaattacg taaaagatat
     2221 gacataccaa caaataaagc accgaagttg ttgttgaagg gtacagggaa tttaaaaggt
     2281 tcatcagttg gatataaaga cattgaattt acgtttgtag agaaaaaaga agagaacatt
     2341 tactttagtg atgggttaat ttttaaacca agtgaggata aataatcatg actcgatcaa
     2401 aatatgaaat caatccagga aagataataa gaagaatagg attgtgaaat tatgaaacga
     2461 ttaaacaaat tggtattgta tattagtttt ttgatattag tcattagttt cactgctggt
     2521 tgtggcatag gtaaagaagc ggaagttaag aaaagctttg aaaaaacatt gagtatgtat
     2581 ccgattaaaa atctagagga tttatacgat aaagaaggtt atcgtgatga cgaatttgat
     2641 aaaaatgata aaggtacatg gattattggt tctgaaatgg ttgttcaacc taaaggggaa
     2701 cgtatgaaat caaaaggtat ggttctatat atgaatagaa acactaagac tacaaccggg
     2761 aagtatatag tgagtgaaac acttcatgat gaagatggta gacctaagag taaagataaa
     2821 gaatatcccg ttaaaatggt agacaataaa atcattccaa ctaaaggtat caaagacgaa
     2881 aatataaaaa aagaaatcga gaattttaag ttcttcgcgc aatatggcag ctttaaagat
     2941 ttgtcgaagt acaaagacgg cgatatatca tataatccag aggtaccgag ttattcagca
     3001 aaatatcaat taactaatga tgactataat gtaaaacaat tacgaaaaag atataaaata
     3061 ccaacgaata aagcaccaaa gttattgttg aaaggttcgg gagacttaaa aggttcatca
     3121 gttggatata aagacattga atttactttt gtggagaaaa aaggtgaaaa tacttttttt
     3181 actgatagtc tacatcttga accgagtgag gataaataat catccacaca cacgattcaa
     3241 tatgaaattc aaatatgttg ctgtaaaata atgtaaaata aacgtattca tattactgaa
     3301 ttgagggata gtatgcaacg tgattattta attcgagtag aaactgagag tatgtcagat
     3361 ttcaaaaggc tcaatagttt aatgattggt tttgttatta aaggtgaggc acacatttat
     3421 gatgaaaata atatgacgca atgcaacagt ggcgacattt tcatcattaa ccaccgcgac
     3481 ttgtatcgat ttcaacttca acaagatggc atcatatgtt atatccaatt ccaaatgaaa
     3541 tatttagcag acaagtttga tgatgtgcat tgtctatatt ttcacttaac agatgcgacc
     3601 acaacaaaga atatacatca actgagaaat ataatggcaa gactggtttc aacacatatc
     3661 cgacataatg agttgtctaa attgactgag caacaacttg tgattcagtt gcttatgcat
     3721 atgattcatt atgtcccgcg tacatatcat tcgaaccaaa gtatcttaaa tgatgataaa
     3781 gtgaatcaag tatgcgacta tatcgagtta cattttcatg aagatttaag cctttcagaa
     3841 ttaagcgaat acgttgggtg gtcagagagc catctgtcta aaaagtttac agaatcgcta
     3901 ggtgtaggat tccatcattt cttaaatacg acgcgaattg agcatgcgaa actcgatttg
     3961 acatacacag atgaaaccat tactgatatt gcattgcaaa atggcttttc aagtgcagcg
     4021 agctttgcga gaacatttaa acactttacg catcaaacac ctaaacaata tcgaggtgat
     4081 cgtccagcaa tcactgaaaa ccagcaatcg gcacaacatg attatcacga ccgtgaattg
     4141 atattacttt taaatgacta cattgaagaa atgaatcatt ttattgaaga tattgaaaag
     4201 atgaactata aagagattgc ctttcaacca accaatcacc aactaaatca atttaatcat
     4261 attattcaag tgggctattt gaggaatttg ctcaatacac agtatcaatc acagttgctt
     4321 acatgtcatc atgattttca agtcaatgaa gtattagcat atgatgtgat tccatatatc
     4381 atgaaaaagc tcaatgcgcc attcacgtat gatgcagaaa tttcgaatat attttatgat
     4441 attgatttgt gtttagactt tttattagat cataacttta gtttgaccat gcatttggat
     4501 cagtatgact cacgtgatta tatcgatgca tttaaaattt ttatccatca cgttgccctg
     4561 catgtcagtc atagaaaaga tttgaagttc aacttgtatg tgacgacatt gcacacgtct
     4621 ttgattgaaa tgattgatta ttttaaagca ttattcccta atggtggctt gtacattcac
     4681 ttagatcaag ctacggaaag acatctacca ttattgaaac gacttgagcc acacatcgac
     4741 cattttgtat ttgatgccaa ttcaaatgac gctgttgatt ttaataaaat gaatgatgat
     4801 gaatttaaaa ccgcgagtca aatgattatt aataaaacga attaccttat cgacttaatg
     4861 catcgtcatc gcctaaagcg tccactcatt ttactcaatt ggaatacatt gacgggtgat
     4921 acatttatta caaacggcga atattttaga ggtggtatca tcattgagca attattaaaa
     4981 ttaagttcta aagtagaggg tatcggttat tggttgaatt atgatttgca cgtcagtcat
     5041 tgtaaaaatg aacgggatta tatgaattct attgaactat ttcatcaata taatggaaaa
     5101 cgtccggtct atttcacggc attgctattt aataaattaa caagcaatat tttgtattct
     5161 gatgatacat gtattgtcac ggggactgat tcaaattttc aaatattgtt atatgatgca
     5221 aagcatttta atccgtactt agcgttggac aatcaaatga atatgcgtgc gacggaaatg
     5281 attcatttga acattaatgc cctggaagaa ggtatgtata agattaaaca ttttacctta
     5341 gataaagaaa atggtgcgtt atttaatctt tggcgcaaac atcatacgat acatggcatg
     5401 gacaaggact ctatagatta cgttaatcga atgagttttc cgaaattaga agtatatgat
     5461 atagatatca cggacacact ggcattaaac attaaaatga ttacgaatgg gattcactta
     5521 attgaagtaa aacgttaccc aagttcataa aatgatcaca aatcacaaat tttgatatac
     5581 ataatttgtg attttttata ttcaaagcta aaattgcaaa aaattaatgg ttaacatctc
     5641 tgttgttggc aatataaata atgattaatc atttatgatg taactaagga gatgaaggat
     5701 atgaatcaac aattaattga aactttaaaa tctaaagaag gcaaaatgat tgagatcaga
     5761 cgttatttac atcagcatcc agaattatct tttcatgaag atgaaacggc gaaatacatc
     5821 gctgaatttt acaaaggtaa agatgtggaa gtagaaacga atgtcggacc acgtggaatt
     5881 aaagtaacga ttgattcagg gaaacctggt aaaacattag caatccgtgc agactttgac
     5941 gcattaccca ttactgaaga tacaggatta tcttttgcat cacaaaataa aggtgttatg
     6001 cacgcatgtg gtcacgatgc acatacagca tacatgcttg tattagcaga gacgcttgct
     6061 gaaatgaaag atagttttac aggaaaagtc gttgtgatac atcaaccagc tgaagaagta
     6121 ccaccaggtg gtgctaaaac aatgattgaa aatggtgtat tagacggtgt tgatcatgta
     6181 ttaggtgtac acgtcatgag cacaatgaaa acaggtaaag tgtattacag acctggttat
     6241 gttcaaacag gacgcgcatt cttcaaattg aaagttcaag gtaaaggtgg tcatggttca
     6301 tcaccacata tggccaatga tgccattgtt gcaggtagct acttcgtcac agcgttacaa
     6361 acagttgtat ctagacgact aagtccattt gaaaccggtg ttgtcacaat cggttcattt
     6421 gacggtaaag gtcaattcaa tgtcattaaa gatgttgttg aaattgaagg tgatgtacgt
     6481 ggattaacag atgctacaaa agcaacaatt gaaaaagaaa ttaaacgttt atcaaaagga
     6541 ttagaggata tgtatggtgt aacttgcacc ttagaatata acgatgatta tccagcatta
     6601 tataatgatc cagagtttac tgagtacgtg gctaagacgt tgaaagaagc aaaccttgat
     6661 tttggtgttg aaatgtgtga accacaacca ccttcagaag actttgcata ttatgctaaa
     6721 gaacgcccaa gtgcctttat ttatacaggt gcagcggtgg aaaatggtga aatttaccca
     6781 catcatcatc ctaaatttaa catttcagaa aaatcattac ttatttctgc agaagcagta
     6841 gggacagttg ttttagatta ccttaaagga gataactaac atgaatgaaa cgtatcgcgg
     6901 gggcaacaag ttaatcttag gtattgtatt aggtgttatt acattttggt tgtttgcaca
     6961 atcacttgta aatgttgtac cgaatttaca acaaagtttt ggtacagaca tggggacaat
     7021 tagtattgcg gtcagtctaa ctgcactatt ttcaggcatg tttgttgttg gagcaggtgg
     7081 tctggcagat aaaattgggc gcgtgaaaat gacgaatatc ggtttattat taagtattat
     7141 tggttcagca ttaattatta ttacgaattt accggcatta ttaattttag gtcgtgttat
     7201 acaaggtgta tcagcagcgt gtattatgcc ttctacattg gccattatga aaacttatta
     7261 tcagggtgct gaacgtcagc gtgccttaag ttattggtct atcggttctt ggggtggcag
     7321 tggtatctgt tcactcttcg gtggtgcagt tgcgacaact atgggttgga gatggatttt
     7381 catcttctca attatcgttg ccgtactttc aatgttactc atcaaaggga cgcctgaaac
     7441 gaaatcagaa attaccaata cacataaatt tgacgttgca gggctaattg ttctagtagt
     7501 tatgttgcta agtttaaacg ttgtcattac taaaggtgca gcacttggtt acacatcatt
     7561 atggttcttt ggtttgattg caatcgtaat tgtagcattc tttattttct taaatgttga
     7621 gaaaaaagta gataatccac ttattgattt taaattattt gaaaataaac catatacagg
     7681 tgcaacgatt tcgaacttct tattaaacgg tgttgcaggt acattaattg tagcgaatac
     7741 attcgtgcaa caaggtttag gttatacagc attgcaggca ggatacttat caattactta
     7801 tttaatcatg gtgttattga tgattcgagt tggtgaaaaa ttattacaaa aaatgggttc
     7861 taagcgacca atgttattag gtacattcat tgtggtcatt ggtattgcac ttatttcatt
     7921 agtattctta ccaggcatat tttatgttat cagttgtgtc gtaggatatt tatgtttcgg
     7981 actaggctta ggtatttatg caacaccttc tacagataca gctatttcga atgcaccgtt
     8041 agataaagtt ggcgttgctt caggtattta taaaatggct tcatcacttg gtggcgcatt
     8101 cggtgtcgca attagtggtg ctgtatatgc tggtgcagtt gctgcaacga gcattcatac
     8161 aggtgcgatg attgcacttt gggttaacgt attaatggga atcatggcat ttatcgcaat
     8221 tttattcgcg attcctaatg atgataaacg tgtcaaagat gcgaaataat aacgatgaca
     8281 cgccaagtgt tttgataatg atataaatga aagtaaagca aattaaatga ggaaatgtat
     8341 agagcagtgc atatagataa atctatgatt ggattaacac taatataaat ataaatgaaa
     8401 atgaaaaacc atccacttaa atttttgata agttaggtgg atggttattt attgcgttgt
     8461 tctaaaaatt tggacattta tatggaaaga actatatata atgtgaaggt tatggcaatt
     8521 cgcttcaatg aaggcgacgt gtaaaagtgt atattaaacg tttcggggtc agactccgag
     8581 aacagtgtcc gtcatgctta ccgcatacta atgaagcatt attatatcag tgtttatagc
     8641 cgatttaaga taataaattt tgctaaacaa aaaagccaac ccatgaatgt tggattggct
     8701 ttttacatgc atctgaatct ctaattttaa aaaaatatga atataaataa gacagtaaaa
     8761 attaaatttc agttgttgca atttcttcat ctgtaggtac atcatcgtta aggccaacaa
     8821 gtgcttcaga aacatttcgt gaatgataac cgatacgttc aagaacacca atcatatcga
     8881 tatatagtaa tccgcctttt gttgtacatt caccacgatt aaggcgttta atatgacctt
     8941 tgcgtagttt atgttcaata ttaaatgatt ctctactacg ttctacaatt tcatcttttt
     9001 tcgttttgtc ataaacatct aacatgtcga tggctttatc aaatgactca gcaacatggt
     9061 tgaataattt atccataccg cgttgtgcat cttctgtaat gcgaatatct tcatcatgtt
     9121 ggcgttttaa ttgagcgaca tactcttctg ttagctctgc tacttttaaa atagagcgat
     9181 tgacatcaaa cataactgct aaacgctcaa cgtctgcctt cgtaatggct tttgtagaaa
     9241 ttctaactaa ataatttcga atgctatcat tgattgtttc aacagcttga tgcttttgtt
     9301 caagcttttt gatcaatttt ttatcgtctt ttgtaatttc gcgaatgtct tcaaacattg
     9361 ataagacaat ctgacccaca ttttgtaatt ctttttgagt ttcttgtaat gcaacaccag
     9421 gtgcgtgata aacaagatct ttgtttaagt gctgaggttt atagtcatca gcaatatctt
     9481 tacctgggac aagctttgta acaatccatg ctaaacctgc tacaaatggt aattgaatca
     9541 aagtatttgt tatgttgaag ataccatgtg atactgcaat cgtcatcgct ggttttaagt
     9601 gccataaatc ttgtaacaaa ctaatcaaat gaatcacaac tggcaagaaa attgtgaaga
     9661 taattacccc gattaagtta aagatgacgt gtacaagcgc cgcacgtttt gcagcgattg
     9721 agccggctaa actagctaag atagctgtaa tcgtggtacc aatgttatcg cctagtaaca
     9781 cagggattgc tgcgtttaag ctaattaaat cttgttgata aaattcttgt aaaataccaa
     9841 tcgtcgcact tgaactttga actagtgctg ttaaccctgc gccgacaatg acagcgagta
     9901 ttggatttgt agacatatca agcattaatt gcttaaatcc atctaatgat gctaaaggtt
     9961 taacggcatc acccataaat tctagaccga agaatagtga accgaaaccg aatagtatgc
    10021 ggccaatgtt attgatttta gagcgtttaa agaaaaagat taagaatgca cctaatgcta
    10081 aaattggcat tgcatattcg cctaaatcta taccgataat aaatgcagtt accgttgttc
    10141 cgatattagc acccattatc actccaatgg cttgtttcaa tgtcataaat ccagctgtta
    10201 ccagtccgat tgtgataact gtcgtacctg aactactttg tattaaaata gttacaacga
    10261 tacctgcaat aacacctaat actggatttg atgtaaattt gtttaaaata tctcgtagcc
    10321 tgtctcctgc tgatgcttga agcccgtctc ccatgatttt taagccgtaa aggaaaatac
    10381 ctaaaccacc taaaaaggag aaaatgactt ctgtaaccga catttccatt atttcacctc
    10441 aaataagctt tatatttaga ttatcgctta taattgtaaa attaatgtta agattaggta
    10501 aaattattta acaatatatg ttatttgtat atgacttgta aaatatcgtc acttattatg
    10561 taaattttca gtgtgaaatg gcaggtttgc aatcacttgt ttaacaaaat gatgcaatca
    10621 atcatgtaat tatgtttcat caaaaaaatc atgagagtgg aacaacgaaa taatttttat
    10681 gaaaacatca tttctgtccc agtctcatga tttgaaatca ccaaataaaa atctattaat
    10741 ggttttcgtt ataacaattt gtgttctttt aataatgact caatgtacgt accttttatc
    10801 tttttaagga atcctgctaa tgcgagtttt tgcattttcg aatctttagt aatctcacgc
    10861 aaatcttggt ggtcattcag ttcgtatatg gcatccatta agacgcgaag atcaaatgga
    10921 ctattgatga cttctggaat accacgatct atatttagta attgataaac agcttccatg
    10981 gcagtacgaa ccgaatattc tgttgtaaat acagtgtctc gctctgtttc tgcaaagtta
    11041 ccaataaatg ctaagttctg agattgatgc gggacgacta aaggtctgtc gccgatagca
    11101 cgcgtcatga aataagatgt gatatatggc atataaacag gaatcgtatt agatgcatgt
    11161 tttgctaagt cttcaatttt gtcagttgat acacctaagt gatacagcca ttcttggcat
    11221 atttcattac cactacattc tgtaattggc tttttaatat aatcgccgtt tacatctgaa
    11281 tataaggcat aaatccatgt agatatttca ttttcaggtt ggtctttaaa ctgttgctga
    11341 cgattgattg taaaactcat ttgccatgca gaatcattga ttgtaataat accgcctgta
    11401 actgttttgc ctgcaagtgg gtcacgttta caaatacttt ctattgtatc gataatctct
    11461 ttattgtttg ttgtagaagt tgctgaaaca aaccaacttt ttttaggaat attttggcaa
    11521 aacttatcag gattaccaaa ttcaggactt tgtcgcgcta aatttttcca tagtgtccaa
    11581 ctaccaccta attcgtcagt tggtggcgct ggtgtatcat tatcaccata agtagagctt
    11641 tctgtaatac taccgtttgt cacaaagaca agatcgttta tagtcagttt aatagattct
    11701 gcattaccat tacggtcaat taatatttct cgggcaattt tttgacttgt cgtaacatct
    11761 attttaatat cttcgacttt tacatcgtat tcaaattgaa ccccatgcga ttttaaatat
    11821 tcaaccatag gtaatactaa agattcatat tgattatatt tagtgaattt taaagctgaa
    11881 aagtctgcga gaccactaat atgatgaacg aatcgcatta gatagcgacg catttccatt
    11941 gcagaatgcc acggttcaaa tgcaaacatc gttttccagt aaatccaaaa gtttgaatta
    12001 aagaagtcat cggaaaatac atctgttatt ttgacatcat ctaaatcttc ttcattcgtt
    12061 aagcataaat ctaaaatttc tttaatcgcc gttttagtca aagtgaagtc tccgtctgtg
    12121 actaaacgtt gaccctgttt ctcaataaca cgacagcgag aatagttagg gtcttctttg
    12181 tttagccaat agaactcatc taatacagac gcgttatcga tttctaatga agggatagat
    12241 ctgaataagt cccacaaaca ttcaaagtgg ttctccattt cacgaccacc gcggacaaca
    12301 tagcctttta aaggcatatt ttcaccatca agactaccac ctgctttagg taactcttct
    12361 aaaatatgaa tcttcgaacc ttccatttga ccatccctta ttaaaaaaca agctgcagca
    12421 agtgaagcta gaccagatcc gattaagtaa gcggatttgt tttctacatt ttcaggtttt
    12481 ttagggcgcg caaatgcttc ataatttcca taactgtaat acatatccac caagtccttt
    12541 cgtattagaa tacactcaga ctatacccct ttgtgaattg caaatattag tatttatatt
    12601 ggtaaatttg acgaaattag atcattttta tatttttgat ttttaaaatt ttatcactcg
//
LOCUS       DAKTLH010000071        12485 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.071, whole genome shotgun sequence.
ACCESSION   DAKTLH010000071 DAKTLH010000000
VERSION     DAKTLH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.071"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            127..942
                     /locus_tag="O0394_001639"
     CDS             127..942
                     /locus_tag="O0394_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002491360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HCY0499719.1"
                     /translation="MHYIKFIESKDNTKLYMKVNDIQDAKANIIIAHGVAEHLDRYDE
                     ITAYLNEAGFSVIRYDQRGHGRSEGKRAFYSNSNEIVEDLDAIINYVKSNFEGKVYLI
                     GHSMGGYTVTLYGTKHPNTVNGIITSGALTRYNNKLFGNPDRNISPDTYIENNLSEGV
                     CSDLEVMEKYKLDDLNAKQISMGLVFSIMDGVRYLKDNAQQFTDNILILHGKEDGLVS
                     YVDSLQLYQEIGSAHKSLHIYDRLEHEIFNESSYNRTIFNEVIEWLETELTYN"
     gene            1264..2442
                     /locus_tag="O0394_001640"
     CDS             1264..2442
                     /locus_tag="O0394_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016913246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCY0499720.1"
                     /translation="MSKRSSVVAHYKNGKFQKEFFIQNNKNGYNYLLKYLNDLDHPQL
                     IFESTGIYSRGMERFCCVNQINYIQMNPLEAKFKTSALRSWKTDQADAHKLACLGPTL
                     KQTGSLPIHELIFFELRERARFHLEIENEQNRLKFQILELLHQTFPGLERLFSSRYSI
                     IALNIAEIFTHPDVVLDIDKDVLITHIFNSTDKGMSMDKATKYALQLRVIAQESYPNV
                     DRHSFLVEKLRLLIQQLKQSIHHLKQLDDAMIQLAQQLDYFENIHSIPGIGKLSTAMI
                     IGEIGDIKRFKSNKQLNAFVGIDIKRYQSGHTHCRDTINKRGNKKARKLLFWVIMNII
                     RGQHHYDNHVVDYYYKLRKQPNEKPHKTAIIACINRLLKTIHYLVMNHKLYDYQMSPH
                     "
     gene            2702..3394
                     /locus_tag="O0394_001641"
     CDS             2702..3394
                     /locus_tag="O0394_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0499721.1"
                     /translation="MTKTYDVWWQKGQESDDDMARDHQEAWERTIKMLDTSDIEGKTI
                     LDVGCNQGGFLRQLYDTTPFKKGVGIDLARLSLEKAETLKGQRPLTYYLTDKPQETKH
                     VFDTAVSTSVLYLIEDIPQHAKDLKEVLKPGGVYYASFADLTNNPSRQFMDDTINQYG
                     ATPSQNHSLKHIVDSFVDAGFEVAVMKEHVPDVIDLTHYSDFYLSPNDYLQTLYEESF
                     LIKASVKEGTEK"
     gene            3391..4944
                     /locus_tag="O0394_001642"
     CDS             3391..4944
                     /locus_tag="O0394_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0499722.1"
                     /translation="MRKCVLMTVAASATLLLAGCGNGQKEDKDVTVSLPTEAKADKLD
                     AQGYDAAMPVYSAVYDALVKYDKDKGIKAGLADKWSVDESGKVYEFHLKKNVKFSDGS
                     ALDAKDVKFSIDRAKAMNKDSTVETLKKLDKVVVKNEHVVQIRLKSPSNQVLNELTQV
                     RPLRIMSPHSVEDGKVNGKFEKAIGTGAFVVDKTGKEKTTMKPNKYFDNGHPVNYHLA
                     FQTIEDGDSRNSAVQSGSVDISGGALGMLSDEQIKQDKKNKNLTIEDRPSTVSHFMAF
                     NPKNDVLNQRTIREAISKSIDAKDIAGKSVNGLFQKNVQFVTKNNQQPHDYDMKAAER
                     LLKSEGYHKNDDGIFEKNGKPLSFNLVIQTAEFPNWKDKAEKVQRQLKQAGIKLNVKT
                     LDSQSYYDTLWTKKDYDLIFYRTYSDALMPYNFISSVFKNNDGQPGVLADDETLTKQL
                     DDFPSTVSKEDQQCSFDDIFKHFNQQYYGVPIAYPNETFVVSDKVKQFKFSGLTDAPI
                     DYKALKVNE"
     gene            4947..5903
                     /locus_tag="O0394_001643"
     CDS             4947..5903
                     /locus_tag="O0394_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0499723.1"
                     /translation="MLKRTIKFILYLIVSSFIIFILVEKTSGNPAILYLQRHGYTSIT
                     QDNIEAAQHQLGLGQHVLLRYIDWVGHALTGNLGYGFSTNEAVTAMIMEAIVPTLVLI
                     IVSSCIMLPFGYIVGYFVGTRPHTRYANGIRGFAQVMTSMPEYWLAILFIYYLGVRWQ
                     LLPFVGSDSWQHFVLPIFTIVVIEGCHILLMTAHLITQTLDQDAYQLAQLRHFSLKAR
                     IIVQIKEIFAPLMTISINSIIHLIGKAVILEVIFSMSGIGKLLINAINQRDYPLIQGI
                     VIFIIVFIMLMNYLGDVIILKNEPRLRRRHTQQSGNEKRGTM"
     gene            5903..6670
                     /locus_tag="O0394_001644"
     CDS             5903..6670
                     /locus_tag="O0394_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0499724.1"
                     /translation="MKKYQTYIAIGSLLSLMVVLITYGLMQDTQHLNPLESPNGQHWL
                     GTDQLGRDFLVRLIVGSLVTLSLTGIVILLSVCMGLIFGLIAGIERRWLDQIIMFVAD
                     MLLAIPSFIIALVILSLVSNSMIGLILALTIGWIGRYLRYFRNLTRDIQKRPFVQYAR
                     LSGNSTFKTTVTHVIPHLLSSIFALVTADFGKMMLSISGLAFLGLGIKPPTPELGTIL
                     FDGKSYFNGAPWLFFFPGVLLGGFALLCQIINKKITQ"
     gene            6637..7404
                     /locus_tag="O0394_001645"
     CDS             6637..7404
                     /locus_tag="O0394_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0499725.1"
                     /translation="MSNYQQKNNAVNTVVNVNQLSILDQEKVLLKDVDLTVTKGAFHC
                     IIGESGSGKSLLTRTILGMKQSQLCYQGDIDIDLTQTDAVFQDVQSNMFQNITLAKHF
                     QYIYEANRTHLTKQRIKEDVLQMMQLLGLRQGEQLLERYPFELSGGMAQRVAFIMSLI
                     RRPNYLFLDEPTSALDQENIKKFMHYLLRAQERYQMTIVFITHDINLVKDCATHISIM
                     QQGKLIENGEAASILTKPTHNYTKKLITIAHRRQPYA"
     gene            7397..8032
                     /locus_tag="O0394_001646"
     CDS             7397..8032
                     /locus_tag="O0394_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0499726.1"
                     /translation="MLKIERLTKYIDTQLIFKEISCTINDQHLLISGESGCGKSTLAK
                     IIAGLDTDYQGELYLNGRLRESYTSKEWMKHIQYVPQYQRDTLNQRKTVLATLLEPLK
                     NYKVNKQRYTSSIEAVLDQCNLPHDILNHKVSTLSGGQFQRVWIAKALILEPEILILD
                     EATTNLDVINEEAILQMLISLKMTQLIIISHDTYVLSQFEGIQLQLNKLNN"
     gene            9052..11115
                     /locus_tag="O0394_001647"
     CDS             9052..11115
                     /locus_tag="O0394_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCY0499727.1"
                     /translation="MNNNGEEHNHQNHMNHSNQMHHDNHASHDHHSGHAHHHGNFKVK
                     FFVSLIFAIPIILLSPLMGVNLPFQFTFPGSEWVVLILSTILFFYGGKPFLSGGKDEI
                     ATKKPGMMTLVALGISVAYIYSLYAFYMNNFSSATGHTMDFFWELATLILIMLLGHWI
                     EMNAVGNAGDALKKMAELLPNSAIKVMDNGQREEVKISDIMTDDIVEVKAGESIPTDG
                     IIVQGQTSIDESLVTGESKKVQKNQNDNVIGGSINGSGTIQVKVTAVGEDGYLSQVMG
                     LVNQAQNDKSSAELLSDKVAGYLFYFAVSVGVISFIVWMLIQNDVDFALERLVTVLVI
                     ACPHALGLAIPLVTARSTSIGAHNGLIIKNRESVEIAQHIDYVMMDKTGTLTEGNFSV
                     NHYESFKNDLSNDTILSLFASLESQSNHPLAISIVDFAKSKNVSFTNPQDVNNIPGVG
                     LEGLIDNKTYKITNVSYLDKHKLNYDDDLFTKLAQQGNSISYLIEDQQVIGMIAQGDQ
                     IKESSKQMVADLLSRNITPVMLTGDNNEVAHAVAKELGISDVHAQLMPEDKESIIKDY
                     QSDGNKVMMVGDGINDAPSLIRADIGIAIGAGTDVAVDSGDIILVKSNPSDIIHFLTL
                     SNNTMRKMVQNLWWGAGYNIVAVPLAAGILAFIGLILSPAIGAILMSLSTIIVAINAF
                     TLKLK"
     gene            11133..11678
                     /locus_tag="O0394_001648"
     CDS             11133..11678
                     /locus_tag="O0394_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1541 domain-containing protein"
                     /protein_id="HCY0499728.1"
                     /translation="MIKKLFFMILGSLLILSACSNNDEKDKDTNDQKSESHMKHNDES
                     KVPEDMKSTNEGEFKVGDKVTITAGHMPGMKGAEATVKGAYKTYAYVVSYKPTNGNEK
                     VNNHKWVVNEEIKDAPKDGFSKGDTVKLEASHMSGMKGATANIDNVKKTTVYVVDYKS
                     KDNGKIIKNHKWMTGNELKAR"
     gene            complement(12106..12240)
                     /locus_tag="O0394_001649"
     CDS             complement(12106..12240)
                     /locus_tag="O0394_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499729.1"
                     /translation="MKLHWYIYYFLSISVILFMIIYHYLATSMTVDYEITVSIAKLLL
                     "
     gene            12204..12296
                     /locus_tag="O0394_001650"
     CDS             12204..12296
                     /locus_tag="O0394_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499730.1"
                     /translation="MTKNSKYTNEVSKEQFQEIENVNNKVKEFY"
BASE COUNT         4499 a         1809 c         2293 g         3884 t
ORIGIN      
        1 taaaaaacat gggcttaaag ggttaattta tatagtatta ctgcaaacat tgatttgggt
       61 caaaaattag gggtattaaa aaaatgaatg aatcataatt ttatcaagct gattggagag
      121 gttaaaatgc attatataaa atttattgag tcaaaagata atacaaaact ttatatgaaa
      181 gtgaatgata ttcaagatgc aaaagcgaat atcattatag ctcatggtgt ggcagaacat
      241 ttagatcgtt atgatgagat aacagcatat ttaaatgaag cgggttttag tgttattaga
      301 tatgatcaaa gagggcatgg tcgttctgaa ggcaagcgtg ccttttatag caatagtaat
      361 gaaattgtcg aagatttaga tgcgataata aattatgtga agtcaaactt tgaaggtaaa
      421 gtttacttaa tcggtcatag tatgggtggt tatacagtca ctttatatgg aacgaaacat
      481 ccaaatacag tgaatggtat tataacttct ggagcattaa cacgttataa taataaacta
      541 tttggcaatc ctgatagaaa catatcacct gatacttata tagaaaacaa tttaagtgag
      601 ggggtatgtt ctgatttaga ggtaatggaa aaatataaac ttgatgattt gaatgcgaaa
      661 caaatctcta tggggctcgt cttttcaata atggatggtg ttaggtattt gaaagacaat
      721 gctcaacaat ttacagataa tattttgata ttgcatggca aggaagatgg gctagtaagc
      781 tatgtagatt ctttacagct ttatcaagaa ataggatcag cacataaatc attacacatc
      841 tatgatcgtt tggagcatga aatatttaat gaaagttctt ataatagaac tatttttaac
      901 gaagttattg aatggcttga aacggaatta acttataact aaaacagtat agttccgtgt
      961 atttgattat aagaaattat gaggatatta aacatactaa gattagctat gaagaaatct
     1021 atgacgatag attttttcat agctattttt tatagttata gagaggagta gactgtccag
     1081 actcttggat tttaaatccg taaaaaaaac aagtcagctt tactctcacc ttttgaaatt
     1141 cgtttgtagt atgttgggtt cttgaaaccg tgtataggaa aatgaaatga gaaaggttaa
     1201 gtaaagtttt tagcttctca actattcaaa ggaggttttt ttatcgatta cttaggtgtt
     1261 gatattagta aaaggagtag tgtagttgca cattataaaa atggaaaatt ccaaaaagag
     1321 tttttcatcc aaaataataa aaatggctac aattatttac tcaagtattt gaatgactta
     1381 gaccacccac aactcatttt tgaatctaca ggtatctatt caagaggtat ggaacgattt
     1441 tgttgtgtaa atcaaattaa ctatattcaa atgaatccgt tagaagccaa atttaaaacg
     1501 agcgctctaa gatcatggaa aactgatcag gcagatgctc ataagcttgc ttgtttagga
     1561 ccgacgctca aacaaacagg cagcttacct atacatgagt taatattctt tgaattaaga
     1621 gaacgtgccc gttttcatct agaaatcgag aatgaacaaa atcgacttaa atttcagatt
     1681 cttgaattac tccatcaaac attccctggt ttagaaagat tatttagtag tcgatattca
     1741 atcattgcac tcaacatcgc agaaattttt actcatccag acgtggttct tgatatcgac
     1801 aaggatgtac ttattacaca tatattcaat tctacagata agggaatgtc aatggataaa
     1861 gctacaaaat atgcacttca attaagagtg attgctcaag aaagctatcc taatgtcgat
     1921 agacattcct ttctagtcga aaaattacgc ttacttattc aacaattaaa acaatctatt
     1981 catcatctca aacaattaga tgatgccatg attcaattag cacaacaact cgattatttt
     2041 gaaaatattc attcgatacc tggtattggt aagctaagca cagctatgat tattggggag
     2101 attggtgata ttaagcgatt taaatcaaat aaacaactca acgcttttgt aggcattgat
     2161 atcaaacgat atcaatcagg tcatacacac tgtagagata ccatcaacaa gcgtggtaat
     2221 aaaaaagcga gaaaactttt attttgggtg attatgaata taataagagg gcagcatcat
     2281 tatgacaatc atgtcgtcga ttattactac aaactaagaa agcagcctaa tgagaaacct
     2341 cataagactg ccatcattgc ttgtataaat cgattattaa aaacgattca ttatctggta
     2401 atgaatcata aattgtacga ttatcaaatg tcaccacatt agccaaacgt acaattaaat
     2461 atattttaat accttattca aaaaattaaa atgaacggtt tagttaagta atgcttattt
     2521 taattataag tacttgacta atcgtaagaa agagcctagg acataaatca atgtctcgcg
     2581 accacagctt aatttttggt gttcattcat gactttaaaa aatccttatt gcataaatgt
     2641 acatagtgta gtactattca aaacgtaatt attacgattt gaattaagcg aggagaatga
     2701 aatgactaag acttatgacg tttggtggca aaaaggtcaa gaatcagatg atgatatggc
     2761 acgagaccat caagaagctt gggagagaac aataaaaatg cttgatacat ctgacatcga
     2821 agggaaaacg attttagatg tgggatgtaa tcaaggcgga tttttacgac agttatacga
     2881 tacaacaccg tttaaaaaag gtgttggcat agatttagca cgtttatctt tggaaaaggc
     2941 agagacatta aaaggacaac gtccacttac atactattta acagataaac cgcaagaaac
     3001 gaagcacgtg tttgatacgg cagtaagtac gtctgtcttg tacttaatag aagatattcc
     3061 gcaacatgca aaagatttaa aagaggtatt gaaaccaggc ggtgtttatt acgcttcatt
     3121 cgcggattta actaataacc caagtcgtca gtttatggat gacacgatta atcaatatgg
     3181 tgcaacacct tctcagaatc actctctaaa acatatcgtt gatagctttg tggatgcagg
     3241 atttgaagtt gcagtaatga aagagcatgt acctgacgtg attgatttaa cacattatag
     3301 cgatttttat ttatcaccga atgattattt acaaacacta tatgaagaat cgtttttaat
     3361 aaaagcaagt gtgaaagaag gtactgagaa atgaggaaat gtgtattaat gacggtagca
     3421 gcaagtgcta cgctcttatt ggcaggttgt ggcaatggtc aaaaagaaga taaagatgtt
     3481 acggtatcgc tacctactga agcaaaggcg gataaacttg acgcgcaagg ctatgatgca
     3541 gcgatgcccg tttatagtgc agtgtatgat gcattagtta aatatgataa agataagggt
     3601 attaaagcag gtttagcaga taaatggagc gttgatgaat cagggaaagt ttatgaattc
     3661 catttgaaaa agaatgttaa attctcagat ggttcagcat tagatgctaa ggacgtgaaa
     3721 ttctcgattg atcgtgcgaa agcgatgaac aaagattcga ctgtagaaac gttaaaaaaa
     3781 ttagataagg tcgttgttaa aaatgagcac gtggtccaaa ttagattgaa atctccttca
     3841 aatcaagtgt taaatgaatt aacacaagtg agaccgttgc gtattatgag tccacattca
     3901 gtagaagatg gtaaagtaaa cggtaaattt gaaaaagcga ttggaacagg tgcatttgtt
     3961 gttgataaaa ctggtaaaga aaaaacgaca atgaagccaa ataaatattt tgacaacggt
     4021 cacccagtca attatcatct tgcattccaa acgattgaag atggggactc aagaaattct
     4081 gcagtacaaa gtggttctgt agatatttct ggtggtgctt taggtatgct ctcagacgaa
     4141 caaatcaaac aagataagaa aaataagaac ttaacgattg aagatagacc tagcacagta
     4201 agtcacttta tggcatttaa ccctaaaaat gatgtattaa atcaacgcac aattcgtgaa
     4261 gcgataagta agagcatcga tgcgaaagac attgcgggca aatctgtaaa tggtctgttc
     4321 cagaagaacg tacaatttgt gactaaaaat aatcaacagc cacacgatta tgatatgaaa
     4381 gcggctgaaa ggttacttaa atcagaagga tatcataaaa acgatgacgg catctttgaa
     4441 aagaatggca aacctttatc atttaactta gtcattcaaa ctgcagagtt cccaaattgg
     4501 aaagataaag ctgaaaaagt gcaacgtcag cttaaacaag ccggtattaa gttaaatgtg
     4561 aaaacgttag attcacaatc atactatgat acattatgga cgaaaaaaga ctatgatttg
     4621 attttctata gaacgtattc agatgcatta atgccttaca actttataag ttcagtgttt
     4681 aaaaataatg atggtcaacc aggggtgtta gctgatgatg aaacattaac gaaacagcta
     4741 gacgatttcc catcaaccgt atcaaaagaa gaccaacagt gttcatttga tgacatattt
     4801 aaacacttta atcaacaata ctatggtgtg ccaattgctt atccaaatga gacgtttgta
     4861 gtgagtgata aagtaaaaca attcaaattc tctggactta cggatgcacc aattgattat
     4921 aaagcgttga aagttaatga atagcaatgc tcaaacgtac aattaaattc atactttatt
     4981 taatcgtaag ttcgtttatt atcttcattt tagttgagaa gacatctggt aatccagcga
     5041 ttctgtatct acaacgtcat ggttatacgt cgattacgca agacaatatt gaagcggcac
     5101 aacatcaact tggcttagga caacatgtgt tactaagata tatcgattgg gttggacatg
     5161 cactcacggg caacttagga tacggcttta gtacgaacga agcagttacc gctatgataa
     5221 tggaagccat cgtgccgacg cttgtgctaa tcattgtctc tagttgtatc atgttgccat
     5281 ttggctatat tgttggttac ttcgttggga cgcgtccgca tacacgttac gctaatggaa
     5341 ttcgtggatt cgcccaagtg atgacctcaa tgccagaata ctggttagct attttattca
     5401 tttattattt aggcgtacgt tggcaattgt taccatttgt aggtagtgat tcatggcaac
     5461 actttgtgct gccaatcttc acaattgttg ttatagaagg gtgtcatatc ttattgatga
     5521 cagcacatct gattacacaa acgttagatc aagatgcgta tcaactggcg cagttaagac
     5581 atttttcgtt aaaagcgcgt atcatcgtac aaattaaaga gatatttgca ccactaatga
     5641 cgatttcaat taacagtatc attcatttaa ttggaaaagc cgtaatacta gaagtcatct
     5701 tcagcatgtc tggtataggt aaattgttga ttaatgctat taaccaacga gattatccac
     5761 tgattcaggg cattgtcatc tttatcattg tctttattat gctaatgaat tatttaggcg
     5821 atgtgattat tttgaagaat gaacctagac ttcgacgacg tcatacccag cagtcaggca
     5881 atgagaaaag aggtacgatg tgatgaaaaa atatcaaacg tacatcgcaa taggttcact
     5941 attgagtttg atggttgtat taattacgta tggtttaatg caagacacgc aacatttgaa
     6001 cccacttgag tcacctaatg gacaacattg gttgggtacc gatcaattag gcagagactt
     6061 cttagtaaga ctgattgtcg gtagtcttgt cacattgagt ttaacaggca tagtgattct
     6121 attaagcgtt tgtatgggac ttatctttgg cttaattgca ggcatagaaa gacgatggtt
     6181 agatcaaatc atcatgtttg ttgccgatat gttgctggct attccgtcat ttattatcgc
     6241 attagtcatc ttaagtttag taagtaactc catgataggt ttgatacttg ctttaacgat
     6301 tggatggata ggacgttatt tacgttactt cagaaattta acgcgagata ttcaaaaacg
     6361 tccatttgtt caatatgcac gattgagtgg gaactcaaca ttcaaaacga cagtaacaca
     6421 tgtgattcca catttattaa gtagtatatt cgctttggta acggctgact ttggcaaaat
     6481 gatgctcagc atatctggac ttgcttttct aggactaggt attaaaccgc cgacgcctga
     6541 gttaggaaca attctttttg atgggaaaag ttatttcaac ggcgcaccgt ggctcttctt
     6601 cttccctggt gtattgttag gaggtttcgc cttattatgt caaattatca acaaaaaaat
     6661 aacgcagtaa atacggtagt caacgtcaat caattatcga ttttagatca agagaaagta
     6721 ttgttaaagg atgttgattt gacagtaact aaaggtgcat ttcattgcat tataggtgaa
     6781 agtggcagtg ggaaatcact gttaacaaga acaatacttg gaatgaaaca atcacaatta
     6841 tgttatcaag gagatattga catcgattta actcaaacag atgcagtgtt tcaagatgtt
     6901 caaagtaata tgtttcaaaa tataacatta gctaagcatt tccaatacat ttatgaagcc
     6961 aatcgcacac atctcactaa acagcgtatt aaggaagatg tcttacagat gatgcaatta
     7021 cttggtttaa gacaagggga acaattgctt gagcgttatc ccttcgaact tagtggaggt
     7081 atggcacaac gtgtcgcctt tataatgtca ttaattagac gtccgaacta cttattttta
     7141 gatgaaccaa cgagtgcact tgatcaagaa aatattaaaa agtttatgca ttaccttctt
     7201 agggcacagg agcgctacca aatgaccatt gtttttatca cacatgatat taacttagtg
     7261 aaagattgtg ccacacatat tagtattatg cagcaaggta aattgataga aaatggtgag
     7321 gccgcgtcga tcttaactaa gccgacacat aattacacga aaaaattaat tacgattgca
     7381 catcggagac aaccttatgc ttaaaataga gagattaacc aaatatatag acacgcaact
     7441 gatatttaaa gagatatcat gtacaattaa cgaccagcac ttactcataa gtggggagag
     7501 tggttgtggt aaatccacat tagccaagat tatcgctggc ttagatacgg attatcaggg
     7561 cgaattatat cttaatgggc gcttacgtga atcttatacg tctaaagagt ggatgaagca
     7621 catccaatat gtacctcaat atcaacgtga tactttaaat cagcgtaaaa cggtattagc
     7681 tacattatta gaaccactta agaattataa ggtaaataaa cagcgttata catcaagcat
     7741 tgaagcagtg cttgatcagt gtaatttacc acacgatata cttaatcata aagtttcgac
     7801 attaagtggt ggccaatttc aacgcgtctg gatagctaaa gctttaatat tagaaccaga
     7861 gattctcata ttggatgaag ctacaaccaa cttagatgtc attaatgaag aagctatact
     7921 tcaaatgttg atttccttaa agatgacaca attaatcatt atttcacatg atacatacgt
     7981 cttaagccaa tttgaaggaa ttcagttaca gctaaataaa ttgaataatt aagatcacaa
     8041 atcttaatat ggtgaatatt taatggtacc taaaaaataa aatttaaact acaatgtcta
     8101 aatccatatg ttgtttcatt agaggattta aaaatgatta taacactaaa agatttcaaa
     8161 ttattatatt taatataaat ttacatatga taaacgaata acaattccaa tataaattat
     8221 tttttgatta tttttattta taactatata ttttatatga aaaatataag ttataataat
     8281 aagtttaata ttgcctcgtg gttctgagct tgaacttatc tctaaatcat tttgagctac
     8341 ttatctatca attcatatat tctataacaa tatttgtgac atcacgtgct atttcatgaa
     8401 gtgattttac gatatcacct tctttagaaa aaatatattt cagtaggcac cgacgtatac
     8461 agaatcattt gagtattaaa aataaaaact agaaaaagaa accgcatcat taactgatac
     8521 gcagaatcat attataaata aaactaaaaa tgaggttgta tataactcac tctgaaattg
     8581 attgaatata tagtatcttt aataaaatgc agctattgtg gcgtagaatt tgagaatcaa
     8641 aaaatgatta atatagtttg aagagactga gcataaatac tagaaaaatg gccagtaaat
     8701 gagtttacta taaactcatt tactggcttc tctaataatt atcaagacaa tttgcgtttc
     8761 taggcatact ttgaaatgcg ctattttctt taagaatatt aatatatgac ttgtgttggt
     8821 aatgataacc ttggttagag tgtattgtca tgtgatagtt aagattagat cttttaatta
     8881 aaacattgtt ttaaatatcg atgacaaggt ctaatgtagg acgtgtagat ataaggttcg
     8941 gaattatata aatccataaa aggagataaa tcgaaaacct ttataccctg ggtgggtata
     9001 tgttatagta taagtagctt tactataaca ttttcattag gaggggttaa tttgaataat
     9061 aatggtgaag agcataatca tcaaaatcac atgaatcatt ccaatcaaat gcatcatgat
     9121 aaccatgcct cacatgatca tcatagtggc catgcacatc atcatggaaa ttttaaagtt
     9181 aagttttttg tttcattaat ttttgcaata cctatcattc ttttatcgcc actgatgggt
     9241 gttaacttac cttttcaatt cacatttcca ggttctgaat gggtagtgtt aatattaagt
     9301 acaattttat tcttttatgg tggtaaaccg ttcttgtctg gtggtaaaga tgaaattgct
     9361 acaaaaaaac caggcatgat gaccttagtt gccctaggta tttcagtagc ttatatttat
     9421 agcttgtatg ctttttatat gaataacttt agtagtgcaa ctggtcatac aatggacttt
     9481 ttttgggaat tagcaacctt aattttaatt atgctattag gacattggat agaaatgaat
     9541 gctgtcggaa atgctggaga tgctttaaag aaaatggcag aactgttacc taatagtgct
     9601 attaaagtta tggataatgg ccaacgcgaa gaagttaaaa tatcagacat catgactgat
     9661 gatatcgtcg aagtaaaagc cggagaaagc attccaacag atggtattat cgttcaagga
     9721 caaacatcta tagatgaatc cctagtcact ggagaatcta aaaaagtaca aaaaaatcaa
     9781 aatgacaacg tcatcggggg ttctattaat gggtctggaa caatacaagt caaggttaca
     9841 gctgtgggag aagatggata tctttctcaa gttatgggac ttgttaatca agcacaaaat
     9901 gataaatcta gtgctgaatt gttatctgat aaagtagcgg gttatttatt ctactttgct
     9961 gtaagtgttg gcgtgatttc ttttattgtc tggatgctca ttcaaaatga tgttgatttt
    10021 gcattagaac gtcttgtaac tgtgttagtc attgcttgtc cacatgcttt aggcttggca
    10081 atacctttag tcactgcacg ttctacttca attggtgcac ataatggttt aattattaaa
    10141 aatagagagt ctgtagaaat agctcaacat atcgattatg taatgatgga caaaactggt
    10201 actttaactg agggtaactt ttctgtgaat cattatgaga gctttaaaaa tgatttgagt
    10261 aatgatacaa tattaagcct tttcgcctca ttagaaagtc aatctaatca cccattagct
    10321 ataagtattg ttgattttgc gaaaagtaaa aatgtttcat ttactaaccc acaagacgtt
    10381 aataatattc caggtgtcgg attagaaggt ctaattgata ataaaacata taaaataaca
    10441 aatgtctctt atcttgataa acataaactt aattatgacg atgacttgtt tactaaatta
    10501 gctcaacaag gtaattcaat cagttattta attgaggatc aacaagtcat tggcatgatt
    10561 gctcaaggag atcaaattaa agaaagctca aaacaaatgg tagctgattt actatcaaga
    10621 aatattacac cagtcatgct tacaggtgac aataatgaag tggcacacgc tgtcgcaaaa
    10681 gaattaggta ttagtgatgt ccacgcacaa ctcatgccag aagataagga aagcattata
    10741 aaagattatc aaagtgacgg taataaagtc atgatggtcg gagacggtat caacgatgcg
    10801 ccgagtctta taagagcgga tattggtata gcaattggtg caggtacaga tgttgcagtg
    10861 gattcaggtg atatcatact tgttaaaagt aatccatcag atatcattca tttcttgacc
    10921 ctttcaaata atactatgag aaaaatggtg caaaacttat ggtggggtgc aggttataat
    10981 attgttgctg tacctttagc agctggtatt ttagcattta ttggcttgat tttatcacct
    11041 gcaataggtg ctattttaat gtctttaagt acaattatcg ttgcaattaa tgcctttaca
    11101 ttaaaattaa aataaaagat aggagtttta ttatgattaa aaaattattt tttatgatat
    11161 taggatcatt actaatatta tcagcttgct ccaataatga tgaaaaagat aaagacacta
    11221 atgaccaaaa aagtgagagc catatgaagc ataatgatga aagtaaagtt ccagaagata
    11281 tgaaatcgac taatgagggt gaatttaaag tgggagataa agtaacgatt acagcagggc
    11341 atatgccagg tatgaaaggt gcagaagcta ctgtaaaagg tgcgtataaa acatatgcct
    11401 atgttgtaag ttataaaccc acaaatggaa atgaaaaagt aaacaatcat aaatgggtcg
    11461 taaacgaaga gattaaagat gcacctaaag atggatttag taagggcgat actgttaaat
    11521 tagaagcaag tcatatgtct ggtatgaaag gtgctacagc caatatagat aacgtgaaaa
    11581 agactactgt ttacgtagtt gattacaaat ccaaagataa tggtaaaatc attaaaaatc
    11641 ataaatggat gacaggaaat gagctgaaag cacgataaaa atctagttct aaattgagaa
    11701 ataaatagat ataaaaatat cctccttaat caataattta aataacttat tattgttaag
    11761 gaggatattt tttagtgtgt aaattaaaaa gaattttaga agaataacat ttatcaaaaa
    11821 actgttcatt accttattaa ttgaaattat ataattaaaa accgcatcat taaccgatac
    11881 gcagaggcgt atcataagta aaactaaaaa attctgtatg aggagataat aatttggagg
    11941 gtgttaaatg gtggacatta aatccacgtt cattcaatat ataagatata tcacgataat
    12001 tgcgcatata acttaagtag tagctaacag ttgaaattaa gccctatcaa attggtttat
    12061 atctaaaatg attaatatag aatgcttctt tttgtcctta ttaaattata aaagtaactt
    12121 tgcaatagaa acagttattt cataatcaac agtcattgac gtagctaagt aatgataaat
    12181 aatcataaat aaaattacag atattgacaa aaaatagtaa atataccaat gaagtttcaa
    12241 aagaacaatt ccaagaaatt gagaatgtaa ataataaggt caaagaattt tattaagatt
    12301 tgaaagagta tcaatcaaga aagatgtagt tttttaataa actatttgga aaataattat
    12361 cataatttaa aaactgacaa tttgcgagac tcataaaatg taataatgga aatagatgta
    12421 aaatataatt aaggggtgta atatgaagat taatatttat aaatctattt ataattttca
    12481 ggaaa
//
LOCUS       DAKTLH010000072        12481 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.072, whole genome shotgun sequence.
ACCESSION   DAKTLH010000072 DAKTLH010000000
VERSION     DAKTLH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12481
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.072"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            27..686
                     /gene="arlR"
                     /locus_tag="O0394_001651"
     CDS             27..686
                     /gene="arlR"
                     /locus_tag="O0394_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HCY0499731.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            683..2038
                     /gene="arlS"
                     /locus_tag="O0394_001652"
     CDS             683..2038
                     /gene="arlS"
                     /locus_tag="O0394_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HCY0499732.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            2322..5120
                     /locus_tag="O0394_001653"
     CDS             2322..5120
                     /locus_tag="O0394_001653"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCY0499733.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPADLALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            5134..6402
                     /gene="sucB"
                     /locus_tag="O0394_001654"
     CDS             5134..6402
                     /gene="sucB"
                     /locus_tag="O0394_001654"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCY0499734.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKK
                     EETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLES"
     gene            6982..7791
                     /locus_tag="O0394_001655"
     CDS             6982..7791
                     /locus_tag="O0394_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0499735.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
     gene            7820..8023
                     /locus_tag="O0394_001656"
     CDS             7820..8023
                     /locus_tag="O0394_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499736.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            8204..8995
                     /locus_tag="O0394_001657"
     CDS             8204..8995
                     /locus_tag="O0394_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCY0499737.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQGDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            9009..10895
                     /locus_tag="O0394_001658"
     CDS             9009..10895
                     /locus_tag="O0394_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HCY0499738.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPSIHSDLDDILVNMFLYLPNFFQNQNSEDNMYLAQRIMYQVDDI
                     LKEDMLNEYYYLPKTLYNTLASPEFDDLKRTDASQVDGQDDTSEDDDNESEKADSKSA
                     DSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGD
                     AVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIER
                     EQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKM
                     AETKKGVVLFHETLKALNIKHEILSFSEDAFDSDEHAQPNIINEIINYDYSTFEKDGP
                     RIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEA
                     VEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLL
                     LKSL"
     gene            11120..12463
                     /gene="brnQ"
                     /locus_tag="O0394_001659"
     CDS             11120..12463
                     /gene="brnQ"
                     /locus_tag="O0394_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCY0499739.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
BASE COUNT         4607 a         1795 c         2230 g         3849 t
ORIGIN      
        1 aaaaagtcgt aatatgaggt gtacaaatga cgcaaatttt aatagtagaa gatgaacaaa
       61 acttagcaag atttcttgaa ttggaactca cacatgaaaa ttacaatgtg gacacagagt
      121 atgatggaca agacggttta gataaagcgc ttagccatta ctatgattta atcatattag
      181 atttaatgtt gccgtcaatt aatggcttag aaatttgtcg caaaattaga caacaacaat
      241 ctacacctat cattataatt acagcgaaaa gtgatacgta tgacaaagtt gctgggcttg
      301 attacggtgc agacgattat atagttaagc cgtttgatat tgaagaactt ttagcaagaa
      361 ttcgtgcaat tttacgtcgt cagccacaaa aggatattat cgatgtcaac ggtattacaa
      421 ttgataagaa cgcttttaaa gtgacggtaa atggcgcaga aattgaatta acaaaaacag
      481 agtatgattt actatatctt ctagctgaaa ataaaaacca tgttatgcaa cgggaacaaa
      541 ttttaaatca tgtatggggt tataatagtg aagtagaaac aaatgtcgta gatgtttata
      601 taagatattt acgaaacaag ttaaaaccat acgatcgtga caaaatgatt gaaacagttc
      661 gtggcgttgg gtatgtgata cgatgacaaa acgtaaattg cgcaataact ggattattgt
      721 taccacgatg attacgtttg tcacgatatt tttgttttgt ttaattatta tttttttctt
      781 gaaagataca ctgcataata gtgagcttga tgatgcagaa cgaagctcaa gcgatattaa
      841 taatttattt cattctaagc ctgttaaaga tatatctgca ttagacttga atgcatcttt
      901 aggtaatttt caagagataa ttatttatga tgagcataat aataaattat ttgagacatc
      961 gaatgataac acagtgagag ttgaaccagg ttatgaacac cgttattttg accgcgtaat
     1021 aaaaaaacgc tataaaggca ttgaatattt aattattaaa gaaccaatta caacgcaaga
     1081 tttcaaaggg tatagcttgt taattcattc actagaaaat tatgataaca tcgtaaaatc
     1141 attgtatatc attgcgctgg catttggagt gattgcaaca attataactg ccacaatcag
     1201 ttatgtattt tcaacacaaa ttactaaacc gcttgtcagt ttatcaaata aaatgattga
     1261 gattcgacga gatggttttc aaaataaatt gcaattaaat acaaattatg aagaaataga
     1321 taatttagca aatacgttta atgagatgat gagccaaatt gaagaatcat ttaatcaaca
     1381 aagacaattt gttgaagatg cgtcacatga attacgaaca ccattacaaa ttattcaagg
     1441 tcatttaaat ttgattcagc gatggggaaa aaaagaccca gcagtattag aagaatcgtt
     1501 aaatatttct attgaagaaa tgaatcgtat cataaaatta gtcgaagaat tacttgaatt
     1561 gactaaagga gatgtaaatg acatttcttc tgaagcacag accgtgcata ttaatgatga
     1621 aattcgctcg cgaatacact cattaaaaca attgcatcct gattatcaat ttgatacgga
     1681 tctgacatct aaaaatctag aaattaaaat gaaacctcat caattcgaac aattattttt
     1741 aatctttatt gataatgcaa tcaaatatga tgtgaagaat aagaaaatta aagttaagac
     1801 aaggttaaaa aataagcaaa aaataattga aattacagat catggaattg gtattccaga
     1861 ggaagatcaa gatttcattt ttgatcgctt ttatcgagtg gataaatctc gttcaagaag
     1921 tcaaggcggt aatggactcg gattatctat tgctcaaaaa atcattcaat taaacggagg
     1981 atcgattaaa attaaaagtg aaattaacaa aggaacaacg tttaaaatca tattttaatc
     2041 atgactgaga cgtcaatcaa agtcatagga tcaatttttt aagtacacat tagctgtgac
     2101 taatgtataa gaacaactat aaaacaaata aacagtggtt ctttatcatt tctgttgtac
     2161 tcccaaaatt tacaataaaa tacatctata aacctagaag aatcaacgct tttgttgatt
     2221 cttcttttta gcagataaat aggtaaatct actttaacaa ataactaaat agtgatatta
     2281 ttacattgta agcgtttcaa catttttgtg gagggtgtaa aatgactaac gaaagaaaag
     2341 aagtttcaga ggctcctgta aacttcggtg cgaatttagg tctaatgtta gatctatatg
     2401 atgacttttt acaagatcca tcatctgtac cagaagattt acaagtctta ttcagcacaa
     2461 ttaagaatga tgactcaatt gtaccagctt taaaaagtac aagtagtcaa aatagcgacg
     2521 gcacaattaa gcgtgtcatg cgtttaattg ataatattcg ccaatacggg catcttaaag
     2581 ccgatattta tcctgtaaat cctccaaaaa ggaaacatgt acctaaatta gagattgaag
     2641 actttgattt agatcaacag actttggaag gtatatcagc aggaattgtt tcagatcact
     2701 ttgccgacat ttatgataat gcttatgaag caattttaag aatggaaaaa cgttacaaag
     2761 gaccaattgc atttgagtat acacatatta ataacaatac cgaacgtggt tggttaaaaa
     2821 gaagaattga aacgccatat aaagtaacgt taaataataa cgaaaaaagg gcactattca
     2881 aacaattagc gtatgttgaa gggtttgaaa aatatcttca taaaaacttc gttggtgcaa
     2941 agcgtttttc aattgaaggg gtagacgcac ttgtaccgat gttacaacgt actattacga
     3001 ttgctgcgaa agaaggtatt aaaaatatac aaataggcat ggctcaccgt ggacgtttaa
     3061 acgttttaac gcatgtctta gaaaaaccgt acgaaatgat gatttcagaa tttatgcata
     3121 cagatccaat gaaattctta cctgaagatg gtagcttgca gttaactgct ggatggactg
     3181 gtgatgtgaa atatcacctt ggtggcatta aaactactga ttcatacggt acaatgcagc
     3241 gtattgcact ggctaacaat ccaagtcact tggaaattgt tgcacctgtt gttgaggggc
     3301 gtacgagagc agcacaagat gatacacaac gagctggggc tccgacgact gatcatcata
     3361 aagcaatgcc aattattata catggcgatg ctgcttatcc tggtcaagga attaacttcg
     3421 aaacaatgaa cttaggaaac ttgaaaggct attctacggg tggttcattg catattatta
     3481 ctaacaatag aattggattt actacagaac caattgatgc acgttcaaca acttattcta
     3541 cagatgtggc caaaggttat gatgtgccaa tattccatgt caatgcagat gacgttgaag
     3601 ctactattga agcaattgat attgcaatgg aatttagaaa agagtttcat aaagacgtcg
     3661 ttattgattt agtaggttat cgtcgtttcg gacataacga aatggatgaa ccatcaatta
     3721 ctaatccagt tccttatcag aatattcgca aacatgactc tgttgaatat gtgtttggta
     3781 aaaagcttgt taatgaaggt gtcatttcag aagatgaaat gcattcattt atagaacaag
     3841 tccaaaagga actaagacaa gctcatgata aaattaataa agctgataaa atggataatc
     3901 cagatatgga aaagcctgca gatcttgcat taccgttaca agcagacgaa caatcattta
     3961 cttttgatca cttgaaagaa ataaatgatg cattgttaac atatccggat ggctttaaca
     4021 ttttgaaaaa gttaaacaaa gttcttgaga agcgtcatga gccgtttaat aaagaagatg
     4081 gtttagttga ttgggcacaa gcagaacaac ttgcatttgc gacaatttta caagatggta
     4141 caccgattcg cttaactggt caagatagtg aacgtggtac attcagtcat aggcatgccg
     4201 tgttacatga tgagcaaaca ggtgaaacat atacaccttt acatcatgtt cctgatcaaa
     4261 aagcgacatt tgatatacac aattctccgc tttcagaagc agcagtagtt ggttttgaat
     4321 acggctataa tgtggaaaac aaaaaaagct tcaatatttg ggaagcacaa tatggtgatt
     4381 ttgcaaatat gtcacaaatg atttttgaca acttcttatt cagttctcgc tcaaaatggg
     4441 gagaacgttc aggattaaca ttattcttac ctcatgcata tgagggtcaa gggcctgaac
     4501 attcatcagc aagattagag cgatttttac aattagctgc tgaaaataat tgcacagttg
     4561 tcaacttatc tagttcaagt aattatttcc acttattgcg tgcacaagcg gctagtttag
     4621 attctgaaca aatgcgacca ttggttgtta tgtcaccaaa aagcttactg agaaataaaa
     4681 cagttgcaaa accaattgat gaatttactt ctggtggatt tgagccaatt ttgacagaat
     4741 catatcaagc ggataaggtt acaaaagtta ttttggcaac tggtaaaatg ttcattgatt
     4801 taaaagaagc attagctaaa aatccagacg aatcagtatt actcgttgcg attgaaagat
     4861 tgtatccatt cccagaggaa gagattgaag cattactagc acaattgcca aaccttgaag
     4921 aagtgtcatg ggtacaagaa gaacctaaaa atcaaggtgc atggttatat gtctatccat
     4981 atgttaaagt gctagttgca gataaatatg atttaagtta tcatggcaga attcaaaggg
     5041 ctgctccagc tgaaggcgat ggagaaattc ataaacttgt tcaaaataaa attatagaaa
     5101 atgcattaaa aaataactag ggggaaataa gtcatgccag aggttaaagt tccagaatta
     5161 gcagaatcta ttacagaagg taccattgca gaatggttga aaaacgtagg ggatagcgta
     5221 gaaaaaggtg aagctattct tgaattagaa actgataaag ttaatgtcga agttgtatct
     5281 gaagaagcag gtgtattatc tgaacaactt gcaagtgaag gcgacactgt agaagttgga
     5341 caagcaattg ctatcatcgg cgaaggtagt ggcaatgctt ctaaagaaaa tagtaacgac
     5401 aatactccac aacaaaatga agaaacaaat aataaaaaag aagaaacaac aaataattcg
     5461 gtagataaag ctgaagtaaa tcaagcaaat gatgacaatc agcaacgtat taatgctacg
     5521 ccttctgcgc gtcgatatgc tcgtgaaaat ggtgtgaatc ttgctgaagt aagtccgaaa
     5581 acaaatgatg tggttcgtaa agaagatatt gataagaaac aacaggcacc ggcatcaaca
     5641 caaacaacac aacaagcatc tgcaaaagaa gagaaaaaat acaatcaata tcctacaaaa
     5701 ccagtgattc gtgaaaaaat gtcacgtaga aagaaaacag ctgccaaaaa attattagag
     5761 gtatctaata atacagctat gttaacaaca tttaacgaag ttgacatgac aaatgttatg
     5821 gaattgcgta aacgtaagaa agaacaattt atgaaagatc atgatggtac taaattagga
     5881 tttatgtcat tctttactaa agcttctgta gcagctttga aaaagtatcc agaagttaat
     5941 gcagaaatcg acggcgacga catgattacg aaacaatatt atgatattgg tgtagctgtt
     6001 tctacagatg atggattatt agtaccattt gtaagagatt gtgataaaaa gaattttgca
     6061 gaaatcgaag cagaaattgc taatttagca gttaaagcac gagagaaaaa acttggctta
     6121 gatgatatgg ttaatggttc atttacgatt acaaatggcg gtatttttgg atcaatgatg
     6181 agtacgccaa ttatcaatgg taatcaagct gcaatcttag gcatgcattc aattattaca
     6241 agaccaattg cgattgatca agatacaatc gaaaatcgtc caatgatgta tattgcatta
     6301 agctatgatc atagaattat tgacggtaaa gaagcagttg gattcttaaa aacaattaaa
     6361 gaattaattg aaaacccaga agacttatta ttagaatctt aatccacaac acaaaatagt
     6421 ttactattcc taaaagcggg attaaatcaa taacaaacag cagtaagatt atttcctagt
     6481 cgaaatatct tactgctgta ctttattttt ataatgattc gtattaaaat gtgggtttaa
     6541 ttttgactac ttaaaaaata tatcatttct attgaaatag actcacaata gaaatatagg
     6601 tagaatagcg tgtttcaata tgctaatgaa tgcattttag ataataacaa acgatgaaat
     6661 atatgaattc tataagcgcc tttagttaat ttaaaatcat ctgaacatga tgtaaattcg
     6721 ctttatggac acgacatata tatgtttttc atgtcctaca aaaaacgaaa tattcaattg
     6781 ctatatacag atattcatta atgacacact caatagcaac cataacaata ttatgttacc
     6841 tatttaaatg aaatagttct tttaaagaaa tacatttttc acatattaat ctataatcaa
     6901 aatcaactga ccgatattct ataatttatg attaaaataa gttataatat aatagtaaag
     6961 ataaagatag aggtggctat aatgtgtgga cttagaagta taacattagg tacaacaaat
     7021 atagaacaga caaaacattt catggttgac atattaggat taaattatga agaacttctt
     7081 gaaaactcaa ttcgtttcgg cgatgcagat ataagcccag gaacaagact tcaatttata
     7141 caagttccaa gtgagcaatt agaagaatct cactttgtgg gtattggatt acgtacacca
     7201 actgactcag gtttagagga gtatgcggaa atattatcga ataaggatat tccatttaca
     7261 acagttaaag aattaaatgg caataaatat ttcagtctcg aagataacaa tggtcatatt
     7321 ttctcaatat attcaaacga gaataattat ggcgttggtt taggtatgcc ttcttttgag
     7381 agtgcggtca atccgttaca tcaagtgcaa ggtttaggac cagtgattct taaagtgaat
     7441 catgtagata ttacaggtca aattttaaca aatatattcg gacttgaagt atttgcagaa
     7501 taccaaccct tcgacaatgc tgactatcat gtccaagtat tcaaagttgg aacgggtggt
     7561 ctaggtggcg aaatacattt aatgcctgta gaaaccgaaa tgacaatgcc ggaatatggc
     7621 gcagtcgatc aagttgagtt tgaaacgaaa gatgcagatt tctttaatca agcgaaatcg
     7681 cgcttagatg aagtggaaat accatatcaa acgcttgagc aagatgatat tgaatcaatt
     7741 agaattactg aaaacagtgg attatcgttt atattcactt tacaaaaata atttttttac
     7801 gatagcgagg acaaaattta tgttacatga aacttggaaa gaacgtacac caatcaagaa
     7861 agtagaagtc attaatacag atgcaaagaa atttacagtt tctgacatgt taacggttgg
     7921 caaacaatat gatgttatca acgaaactga agaatattat caaataatag ataattctgg
     7981 attagttggc ggttactata aaacatattt caaagaagtt taaacgcata attattgaaa
     8041 tgaagcctaa tatgagattt tcaattagta aatttggtaa aatataaaca attcgcatac
     8101 taagcattgc gttttatatc agaatagaat agtaaactaa ggcatttgga gaacttattc
     8161 catgtcttag ttttttaatt tatttaaagg agaacgtttt aaaatggcac taaaacatta
     8221 taagaattca gattcaacag ttttcaatga tgcgaaggca ttatttgatt taaataaaaa
     8281 tattttactt aaaggtccaa caggttcagg gaaaacaaag ttggcagaaa cattaagtga
     8341 agttgttgat acacccatgc atcaagtcaa ttgttctgtt gatttagata cagaaagctt
     8401 attaggcttt aaaacaatta aaacaaatgc ggaaggtcaa caagaaattg tctttgtaga
     8461 tggtccagtt attaaagcta tgaaagaggg gcatatttta tatattgatg aaataaatat
     8521 ggctaaacct gaaacattgc ctgtattaaa tggggtctta gattatcgtc gtcaaattac
     8581 gaatccatac actggtgaag taatcaaagc tgtaccagga tttaacgtta tagcagcgat
     8641 aaatgaaggt tatgttggta ctttgccaat gaatgaagca ctaaaaaatc gctttgttgt
     8701 tattcacgtt gattatattg atggggacat tttaaaaaat gtgattaaag agcaaagttt
     8761 attacaaggt gataaacaaa tcgaacaaat tattaagttt aatgaagatt tacgtactat
     8821 gtctaagcag ggacaaattt ctgaagaagc cgctagtatc cgtgcattat tagacttgtg
     8881 tgatttaatc actgtaatgc cagttgaacg tgcaattaaa cgtacaatta ttgataaatt
     8941 ggaagatgaa cgtgaacaac aagcaatata taatgctgta gaactaaact tttaaagagg
     9001 gataaaacat gagtgatcgt ttcataaaat ttaatgacga acagttagat gccaagcaag
     9061 taatgatgtt gcaagactta gctcgactct tattaaaaaa tgaacaaaca caagttaaaa
     9121 tccaaaaatt cccttactat aatcctgttc aaaatgtttt gattacgagt tggttttggt
     9181 cacatcgacc aagccatatc gaaatggctg gtttaaaaac agatgttatg ttagctgctt
     9241 acggttatca tatgatggat gtacaaattg taaatgaagt agtacaagat aaaactttta
     9301 aacaccctaa attttatcaa caacttttta aattattaga agatatgcgc gtattaaatt
     9361 ctattaaagt tgagcgccca agtactgcta aattaattga tcttcgctta gatacacgta
     9421 tatcatatac ggaatctcaa attaaagtgt atcgtactaa aacacaatat acagatttat
     9481 tatttttgta tttagaacat gcatttttaa gccaagattt ctttgatata ccatctatac
     9541 attcagattt agatgatatt ttagtaaata tgttcttata tttacctaat tttttccaaa
     9601 atcaaaattc tgaagataat atgtatctgg ctcaaagaat tatgtatcaa gtagatgata
     9661 ttttaaaaga agatatgcta aacgaatatt attacttacc taaaacacta tataatacat
     9721 tagctagtcc agagtttgat gatttaaaga gaactgatgc aagtcaagtc gatgggcaag
     9781 atgatacttc agaagatgat gataacgaaa gtgaaaaagc tgactctaaa tctgcagata
     9841 gtgagtcaaa aggtggcgcc tatttagaaa tggagcttca cgaaggacaa aatagtgaaa
     9901 cattaggtaa tgatgaagca cgtgaaggcg atgctacaga tgatatgact gatatgatga
     9961 ccaaaaaggg caaaggatca aacgatacgc taaatcgtga agaaggcgat gctgttggtc
    10021 aaagccaagc atttcaactt gatggtgtga acaaaaatgt agaaataaaa tggcaaattc
    10081 ctgaaattga gccacaatat gttttagaat atcaagaatc gaaacaagac gtacaatatg
    10141 aaatcaaaga cttaattcaa attataaaga aaacgattga acgagaacaa cgagatgcac
    10201 gttttaattt aacaaaaggg cgattacaaa aggatttaat taattggttt atcgacgatc
    10261 aatataaatt gttttacaaa aaacaagatt taagtaaatc atttgatgcg acatttacat
    10321 tgttaattga tgcgtcagca agtatgcatg ataaaatggc tgaaacgaag aaaggtgtag
    10381 tgttattcca cgagacgtta aaagcattga acattaaaca tgaaatttta tcattcagtg
    10441 aggatgcctt tgattcagac gagcatgcac aaccaaatat cattaatgaa attattaact
    10501 atgattactc aacctttgaa aaagatggcc cacgtattat ggcacttgaa cctcaagatg
    10561 ataatcgtga tggtgttgct attagagttg caagtgaacg attaatgcgt cgaaatcaac
    10621 atcaacgatt tttaatcgta ttttcagacg gcgaaccgtc tgcatttaat tatagtcaag
    10681 atggtattat tgatacgtat gaagctgtag aaatgtcacg taaatttggt attgaagtat
    10741 ttaatgtatt tttgagtcaa gatccaataa ctgaagatgt tgaacaaaca attcataata
    10801 tttatggtca atatgctatt ttcgttgaag gtgttgcgca tttacctgga catctctctc
    10861 cattattaaa aaaattacta cttaaatctt tataaataaa tttaaaatat gagtttatat
    10921 gaaattgtga ataaaagtca tcagttacta tttaaaaagt ggatttgaag aattttattt
    10981 tctgacataa tttgatttaa acaacacatg tgttttgttt ctgctatgta caacatattt
    11041 catatagact ctttattatt ttttgtgcta aaatatttac ttgtaaatta tttcgtgttt
    11101 taaaaaaagg agtaactcta tgaataaaaa tacatgggtc attgggttta cgctcttcgc
    11161 tatgtttttt ggcgcaggca atctaatttt tcctcctaat ctcggattgg atagtggtca
    11221 atttttctgg cccgccattt tagcgtttgt tctaactggg attggtttac cattattagg
    11281 tgtgattgta ggtgcacttg ataaagaagg atatattggc gcattaaata aaatttcacc
    11341 taaattttca atattgttct taatcatcat ttatttgact ataggaccac tttttgcaat
    11401 acctagaact gcatctacat cttttgaaat gacaattaca ccaattatac atagcaatag
    11461 tagtatcgct ttatttatat ttacgattat ctacttcata gtcgttttgt atatttgttt
    11521 aaatccatct aagttaatcg atcgtattgg ttcattatta acaccattat tattgattac
    11581 tattttagcg atgattatta aaggatactt agactttagc ggtaatagtg ctggaaaggg
    11641 caatgaagca ctatatcatt ctaatttttc aagttttgct gaaggcttta cacaaggcta
    11701 tttaacaatg gatgccattg cagcaattgc tttttcaatg attgttgtta atgcagtaaa
    11761 actaacaggc attactaaaa caaatcaaat attcaaacaa actttgactg ctggtttaat
    11821 tgcagccgta gctttaattt tcatatatat ttcattaggt tatattggta atcatatgcc
    11881 agtaagtgac atgacgttag atcaattgaa atccaaagat cgaaacattg ggacatattt
    11941 attaacgaca atggcttcaa caggatttgg ttcattcgga aaatatttat tgggcatcat
    12001 tgtggcgctg gcatgtctaa ctacagcatg cgggcttatt gttgcagttt ctgaatattt
    12061 ccatagaatc gtacctaaag tatcatacaa agcatttgta ttagttttca ttttaatgag
    12121 ttttattatt gctaaccaag gtttaaatgc tgttatctca atgtcaattc cggtattaag
    12181 cattgtatac ccagtagcaa taactgttgt attattaatt ttaattgcca aattcatacc
    12241 gacaaaacgc atttcacaac aaattccagt tattatcgta tttatattgt cgattttcag
    12301 tgttattagt aagttaggtt ggctgaaaat taactttata gaatcattgc ctctaagagc
    12361 gtattcttta gagtggttcc cagtagcaat tattgcaacg atattaggct atctagtcgg
    12421 catatttgta aaacaagatc caattaaata tcaacaggaa taacgaataa tataaaagag
    12481 g
//
LOCUS       DAKTLH010000073        12440 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.073, whole genome shotgun sequence.
ACCESSION   DAKTLH010000073 DAKTLH010000000
VERSION     DAKTLH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12440
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.073"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(74..181)
                     /locus_tag="O0394_001660"
     CDS             complement(74..181)
                     /locus_tag="O0394_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499740.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHANNKLLKHSLI"
     gene            complement(218..541)
                     /gene="isdG"
                     /locus_tag="O0394_001661"
     CDS             complement(218..541)
                     /gene="isdG"
                     /locus_tag="O0394_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HCY0499741.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(560..1294)
                     /gene="srtB"
                     /locus_tag="O0394_001662"
     CDS             complement(560..1294)
                     /gene="srtB"
                     /locus_tag="O0394_001662"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HCY0499742.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQEHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDRIM
                     TLSTCEDAYSETTKRIVVVAKIIKVS"
     gene            complement(1356..2321)
                     /gene="isdF"
                     /locus_tag="O0394_001663"
     CDS             complement(1356..2321)
                     /gene="isdF"
                     /locus_tag="O0394_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HCY0499743.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(2334..3212)
                     /gene="isdE"
                     /locus_tag="O0394_001664"
     CDS             complement(2334..3212)
                     /gene="isdE"
                     /locus_tag="O0394_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HCY0499744.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(3209..4285)
                     /gene="isdD"
                     /locus_tag="O0394_001665"
     CDS             complement(3209..4285)
                     /gene="isdD"
                     /locus_tag="O0394_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HCY0499745.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTIKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            complement(4285..4968)
                     /gene="isdC"
                     /locus_tag="O0394_001666"
     CDS             complement(4285..4968)
                     /gene="isdC"
                     /locus_tag="O0394_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HCY0499746.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            5177..6229
                     /gene="isdA"
                     /locus_tag="O0394_001667"
     CDS             5177..6229
                     /gene="isdA"
                     /locus_tag="O0394_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HCY0499747.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
     gene            6432..8369
                     /gene="isdB"
                     /locus_tag="O0394_001668"
     CDS             6432..8369
                     /gene="isdB"
                     /locus_tag="O0394_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HCY0499748.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEE
                     TGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEV
                     KAPKETKEVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKKDG
                     TQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVK
                     DYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAE
                     KLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQN
                     VQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYW
                     KDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVD
                     KEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPS
                     VEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVV
                     QTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLP
                     LMALLALSSIVAFVLPRKRKN"
     gene            complement(8523..8696)
                     /gene="rpmF"
                     /locus_tag="O0394_001669"
     CDS             complement(8523..8696)
                     /gene="rpmF"
                     /locus_tag="O0394_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCY0499749.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(8776..9333)
                     /locus_tag="O0394_001670"
     CDS             complement(8776..9333)
                     /locus_tag="O0394_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCY0499750.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            9460..10599
                     /locus_tag="O0394_001671"
     CDS             9460..10599
                     /locus_tag="O0394_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCY0499751.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKNAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTDSLKNIYTVTEGFENRLKSNIYEATDFHHFV
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTSNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            complement(10661..11143)
                     /gene="coaD"
                     /locus_tag="O0394_001672"
     CDS             complement(10661..11143)
                     /gene="coaD"
                     /locus_tag="O0394_001672"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCY0499752.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(11145..11687)
                     /gene="rsmD"
                     /locus_tag="O0394_001673"
     CDS             complement(11145..11687)
                     /gene="rsmD"
                     /locus_tag="O0394_001673"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCY0499753.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            11757..12146
                     /locus_tag="O0394_001674"
     CDS             11757..12146
                     /locus_tag="O0394_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499754.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            complement(12149..12403)
                     /locus_tag="O0394_001675"
     CDS             complement(12149..12403)
                     /locus_tag="O0394_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCY0499755.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
BASE COUNT         4361 a         2184 c         1654 g         4241 t
ORIGIN      
        1 atatcattat taattctttt cttcttcaaa ataaatcaaa atgatataat tgatgattat
       61 tttcaaagca cattcaaatc aaactatgtt ttagcaattt gttgttagca tgtttgtgtt
      121 cattaaaaaa acgaccatca tcggtatcat gtatggtcgt tacaaaagct aacaatacca
      181 attgtcataa caagtactgc aacctcttta aattcaatta tttcatgtaa ctatagccta
      241 tatcatatgt aattactttg ttatttataa tcgggctact ttcatcttca tttttacttc
      301 taacatgttt atgcgctgct ttaaagacat cagattttaa ccaatccgta aaagcttgct
      361 ttgatttcca aactgttaaa attttcactt catcaaaatc ttcttgttct aaagtttgtg
      421 taacaaacat gccatcaaag ccttctaatg tttcaatccc atgtctcgtg taaaatcgtt
      481 ctataatatc ttttgctgtt ccttttgtta acgtcagcct attttctgcc ataaatttca
      541 taattatcct cttttctgtt taacttacct taattatttt tgcgacaaca acaattcttt
      601 tcgtcgtttc actatatgca tcttcgcacg ttgataaagt cattattcta tcttttaccg
      661 ttacattaac atctgaatta attacagatt tacgttttgt ctcatctaaa aattgttgat
      721 aatcttgatc attttcaaaa tctgtacgta tgtaattatc tttagtagta gttttatatg
      781 cactaaatac ttgcaattga tatttaccat atttattgtc aaattcaatt atcttgtgtt
      841 tttcataaaa cgattgcttt aaataatctt ctaacacatc aaacatcgta ttatcaccga
      901 catggtgccc gtataaaata gtattatgat ttaaattctt caattcattt ctaaaatcca
      961 taaaaatact acctttacgt cgatgttctc gctcaaaatc taaatttaaa taatcgtgat
     1021 ttgtcttacc ttgtagtact ggataattta atgatgttcc tgataatttt atccatccaa
     1081 caatgtcttt atttattttt tcaagtgatt caaattgtgg tctcacatgt tcttgatgtt
     1141 tgctcatcag catttgaaat ttttgttgta atttctcata atttgcgcgt tcttgcttgt
     1201 cttcaatata tgtttgaaca attttgtaac caaaaatgat aataattaca accaataaaa
     1261 tttgtacaat agttaaaaat cgcttcattc tcataaaaat cctcttttat taacgacgtt
     1321 tcttcagtca tcactaaacc agttgttgta ccgttttaga ttcgatttcg ttgactttga
     1381 caaattaagt aaattagcat tggaccaccg acaatcatta aaatagcatt ggctggaatt
     1441 tctaaaggag gctgtatcac tcgtcctaat aaatcagcca ctaacaatag ccatgcacca
     1501 ataactgtag aaaacggaat aagtactctg taattgcccc caactagctt tctaaccaca
     1561 tgtggcacaa taatacctaa aaaggctagt tgtccaacaa tcgcaacagt tgcacttgct
     1621 aaaaatactg ctaataaacc tgttaaccat ctgtaacgat caatattaaa accgatactt
     1681 cgcgcttgta tgtcgtctaa atttagtaaa ttcaatttag gggacaatag taatgttaat
     1741 attaatccca ataatgctga tactgctaat atgtatacgt cgctccatat tttcattgtt
     1801 aagccttgag gaattttcat taaagggttt tgagttaaaa tttctaaaac accatttaat
     1861 aatacgaata acgcaacacc tactaatatc atacttacag cattgaatct aaatttagaa
     1921 tgcaacaata taattattaa aaatggtatt aaacctccaa taaaacttaa taatggtaag
     1981 taaaagtaca attgtggaat aaacaacata caaagtgctc tcattataag tgcacctgag
     2041 gaaacgccaa tgatattcgc ctctgccaaa ggattttgta gtgctgcttg taataatgct
     2101 ccagaaactg ctaacattgc gccaaccatc aatgcaatta atatacgtgg caatcgcaaa
     2161 tcaatgattg aatccactgc ttcattgcta ccagttgtaa attttgtaaa taggtcatta
     2221 aatgacaatt taattgtacc ggttacaaac gaaatataag cagttgcgat taaaatgact
     2281 aacaaacata aaaatagtag tttcttttta ttttttatca tcatatcaac tcactatttt
     2341 ttatccttat aaaataaatc atataattga gtcattgcct tatcagcatc aacatttgct
     2401 gtaataccga atggcacttc ctctaagtca taaacatgat tatttttcac agctttaaaa
     2461 tgtttccaaa tatcattttg tttaaattct ttttgaaaca ttttcttaac ttcttcaggc
     2521 attccgtgtg gtaatcgtaa aataatatct ggattgatat tcaacaaatt ttcagtatta
     2581 gacgaaatat attgacgatc tttcacttta ataacatttt ctccacctgc tattttaact
     2641 aaatcaccaa tatatgattt atcagttgct actaaatagc tacccggtac acccattaat
     2701 attaatactt tgggatgttt cttttgttta gctgctttat tttcaatttt ttgctttaca
     2761 gaatttaaat ggtcatttaa ttcttttgct tgtgctttac gattaaattg atcacctaat
     2821 tgtgtaatcg acttttgcat cccttttaaa ctatcaaaat cataaaagta gcctttcata
     2881 tttaattgtt tgtaaaatgg ttgcatttca tctttaatcg ttgacacact caaaacatgt
     2941 gttggtttta actttttaac agcttcaaca ttcggctcca ttggttgacc aatttccggt
     3001 acatctttat aacgattagg caatgtctta tatgacgtgg gtttgccgac aattggtaaa
     3061 tccaatttgt ccaatgtcat tgtcaatgca acagttgttg gtacgattct gaattcgccg
     3121 gatttagttg attcttgaga actggaagat tgacagctgg ttaagataac gacgcttatc
     3181 actaaaatgg ttaaatactt tatgattctc aaacttggtt acctctcttt ctgctgtata
     3241 aatgtgccga aataagtaaa atagttgcta acacaatgcc agctgcaaat aacatatatt
     3301 gctttttatg atttggttca tcaagttctt taatatcact agataattga ctactattct
     3361 tgctatcatc atctctatta aaagctggta acgctttttc tttttcaact acttttttct
     3421 tttccttttt tgatttttca tctttatgtt tcggttgctc tttgtgatta ttttcaacct
     3481 tttttattgt tggagggcta ttttcaactt ttaatgtacc tgatgcttta ggtcgctcat
     3541 tttcaatctt tttattactg tcttgttgca ctttatcttt ttgtacagga atatcattta
     3601 atcctttttc agtaaaaagg ctagtacgtc tttccttagt atctgatttt tgactatctt
     3661 ttttgtcatc aatatctttt ttaagctgat cttgattatt ttcttgattt tgatgttttg
     3721 aaggcacttc ttttgcatca tcatgttgta cttttttctc tttaaaatga atagaataca
     3781 ccatttcttt gttatctaga tttttctttt cagccaacgt tttaacatgc atattgattt
     3841 tatccatatg ctgttcaatt aaaattctta ctattgtgtc atccttttca tcttttataa
     3901 caaaaggaaa ttcttcattt ttgtcatttt gaaaaacaag ttgttcttta tctatttgac
     3961 cttttagctt tacttgcaag taaattttac cagtcatatc tgaaatcaat tgcgcctttt
     4021 cgtcaactag ttgttgttcc tcagttacat ttttattttc ttgattaaaa attagtaact
     4081 ctaacgcttt attctctttt atcttttcat caaaaattga tgtagttaat tctgttttat
     4141 cttttgcttc aggttgatta aatttggctg atggatattt tatctcatca gccaacacgc
     4201 taccaatcat tgttgtatat gtaagtatac ctaagctaat aatagctata atagattttg
     4261 tagcaatttg tttaacattt ctcattattc cacattgcct ttagattttt ttctaacaaa
     4321 caatgtgatt gcgattaata atgctaagga tgcaactggt attgcatata tatatgcagg
     4381 tgtaccagca tttgtttgtg gattttctac tttgtcttta ttcgaactat tagattcact
     4441 ttgaccagta gtcgttccat cagatccttt gtcactacct gaagcagaat ttttatcatc
     4501 tttacctggt gcattagcac ctgctacatc agttggtcca ttaaatttat atgtaatgtt
     4561 gtaatgatgg tcatatttga atggctttcc atttactttt tcatcgatat aaacgtcaat
     4621 ttttccatct attttaccgt tcaacttact tacttcaaat tcagaagtgc gttcatcttt
     4681 ggcagtgttt ttactaataa tattttcttt atgtccttcg atactcattc cagtaatcca
     4741 atgactgtgg ttgacagtta tttgaacata caatttacca tttttcttaa tgtactttgc
     4801 cggtttatta aaatagtcat tagcaattga cgtgtcattg gtattgtatt tgtaaacctc
     4861 ataattcaaa gtaccgctat ctgcggcatt tgcagaatta ctgaatgtcg cgatgatgat
     4921 aattaacgct aaaatcgttg tattaaaaac ttttaaaata tttttcaaaa cataatcctc
     4981 ctttttatga ttgcttttaa gtctttagta aaatcataaa taataatgat tatcattgtc
     5041 aatatttatt ttataatcaa tttattattg ttatacgaaa atagatgtgc tagtataatt
     5101 gataaccatt atcaattgca atggttaatc atctcatata acaacacata atttgtatcc
     5161 ttaggaggaa aacaacatga caaaacatta tttaaacagt aagtatcaat cagaacaacg
     5221 ttcatcagct atgaaaaaga ttacaatggg tacagcatct atcattttag gttcccttgt
     5281 atacataggc gcagacagcc aacaagtcaa tgcggcaaca gaagctacga acgcaactaa
     5341 taatcaaagc acacaagttt ctcaagcaac atcacaacca attaatttcc aagtgcaaaa
     5401 agatggctct tcagagaagt cacacatgga tgactatatg caacaccctg gtaaagtaat
     5461 taaacaaaat aataaatatt atttccaaac cgtgttaaac aatgcatcat tctggaaaga
     5521 atacaaattt tacaatgcaa acaatcaaga attagcaaca actgttgtta acgataataa
     5581 aaaagcggat actagaacaa tcaatgttgc agttgaacct ggatataaga gcttaactac
     5641 taaagtacat attgtcgtgc cacaaattaa ttacaatcat agatatacta cgcatttgga
     5701 atttgaaaaa gcaattccta cattagctga cgcagcaaaa ccaaacaatg ttaaaccggt
     5761 tcaaccaaaa ccagctcaac ctaaaacacc tactgagcaa actaaaccag ttcaacctaa
     5821 agttgaaaaa gttaaaccta ctgtaactac aacaagcaaa gttgaagaca atcactctac
     5881 taaagttgta agtactgaca caacaaaaga tcaaactaaa acacaaactg ctcatacagt
     5941 taaaacagca caaactgctc aagaacaaaa taaagttcaa acacctgtta aagatgttgc
     6001 aacagcgaaa tctgaaagca acaatcaagc tgtaagtgat aataaatcac aacaaactaa
     6061 caaagttaca aaacataacg aaacgcctaa acaagcatct aaagctaaag aattaccaaa
     6121 aactggttta acttcagttg ataactttat tagcacagtt gccttcgcaa cacttgccct
     6181 tttaggttca ttatctttat tacttttcaa aagaaaagaa tctaaataaa tcatcgtcac
     6241 actcataact taatatattt tttattttaa attttattta acctatgtca tagatatttc
     6301 ataatctata acataggtta tttttttata aaataacgtt gcaattaact aacatttcaa
     6361 tgtacaatac aagtaatcaa ttgataatga ttatcagttg ataatataca attaggagtt
     6421 gtttctacaa catgaacaaa cagcaaaaag aatttaaatc attttattca attagaaagt
     6481 catcactagg cgttgcatct gtagcaatta gtacactttt attattaatg tcaaatggcg
     6541 aagcacaagc agcagctgaa gaaacaggtg gtacaaatac agaagcacaa ccaaaaactg
     6601 aagcagttgc aagtccaaca acaacatctg aaaaagctcc agaaactaaa ccagtagcta
     6661 atgctgtctc agtatctaat aaagaagttg aggcccctac ttctgaaaca aaagaagcta
     6721 aagaagttaa agaagttaaa gcccctaagg aaacaaaaga agttaaacca gcagcaaaag
     6781 ccactaacaa tacatatcct attttgaatc aggaacttag agaagcgatt aaaaaccctg
     6841 caataaaaga caaagatcat agcgcaccaa actctcgtcc aattgatttt gaaatgaaaa
     6901 agaaagatgg aactcaacag ttttatcatt atgcaagttc tgttaaacct gctagagtta
     6961 ttttcactga ttcaaaacca gaaattgaat taggattaca atcaggtcaa ttttggagaa
     7021 aatttgaagt ttatgaaggt gacaaaaagt tgccaattaa attagtatca tacgatactg
     7081 ttaaagatta tgcttacatt cgcttctctg tatcaaacgg aacaaaagct gttaaaattg
     7141 ttagttcaac acacttcaat aacaaagaag aaaaatacga ttacacatta atggaattcg
     7201 cacaaccaat ttataacagt gcagataaat tcaaaactga agaagattat aaagctgaaa
     7261 aattattagc gccatataaa aaagcgaaaa cactagaaag acaagtttat gaattaaata
     7321 aaattcaaga taaacttcct gaaaaattaa aggctgagta caagaagaaa ttagaggata
     7381 caaagaaagc tttagatgag caagtgaaat cagctattac tgaattccaa aatgtacaac
     7441 caacaaatga aaaaatgact gatttacaag atacaaaata tgttgtttat gaaagtgttg
     7501 agaataacga atctatgatg gatacttttg ttaaacaccc tattaaaaca ggtatgctta
     7561 acggcaaaaa atatatggtc atggaaacta ctaatgacga ttactggaaa gatttcatgg
     7621 ttgaaggtca acgtgttaga actataagca aagatgctaa aaataatact agaacaatta
     7681 ttttcccata tgttgaaggt aaaactctat atgatgctat cgttaaagtt cacgtaaaaa
     7741 cgattgatta tgatggacaa taccatgtca gaatcgttga taaagaagca tttacaaaag
     7801 ccaataccga taaatctaac aaaaaagaac aacaagataa ctcagctaag aaggaagcta
     7861 ctccagctac gcctagcaaa ccaacaccat cacctgttga aaaagaatca caaaaacaag
     7921 acagccaaaa agatgacaat aaacaattac caagtgttga aaaagaaaat gacgcatcta
     7981 gtgagtcagg taaagacaaa acgcctgcta caaaaccaac taaaggtgaa gtagaatcaa
     8041 gtagtacaac tccaactaag gtagtatcta cgactcaaaa tgttgcaaaa ccaacaactg
     8101 cttcatcaaa aacaacaaaa gatgttgttc aaacttcagc aggttctagc gaagcaaaag
     8161 atagtgctcc attacaaaaa gcaaacatta aaaacacaaa tgatggacac actcaaagcc
     8221 aaaacaataa aaatacacaa gaaaataaag caaaatcatt accacaaact ggtgaagaat
     8281 caaataaaga tatgacatta ccattaatgg cattattagc tttaagtagc atcgttgcat
     8341 tcgtattacc tagaaaacgt aaaaactaat aaatcgtctt tatatttaat tattaaatta
     8401 acaaatttta attggcggat gaggtatcca gttacctcgt tcgccaatta tttttcgcaa
     8461 tataaaaagt cccacttaaa acaatcattt taagcgggac tttttatatt gagtaactaa
     8521 aattatttag ctgctacttc ttcgccattg taagaaccac agtttttaca tacacggtgt
     8581 gataatttgt attcgccaca gtttgggcat tcagtcatac ctggtactga aattttgaaa
     8641 tgcgtacgac gtttgttttt tctagtttta gaagttcttc tttttggtac tgccatgata
     8701 tatcctcctt agattataaa cgaaaaatac taaatgttag tttaattaac aacattatat
     8761 cattaattaa actacttatt gctctttatc atataattgt tgtaattttt gaagccttgg
     8821 atcaacttgt cgtgattctg aatcatcttg ttcttgctgt ttagcaagct catctaattg
     8881 atcctcatcg attacttccc aaccattacc tactgtcaac atttggtcac tttgctctga
     8941 ataagctctc attggtttct caataataac tatatcctcg acaatatcct gaagattaac
     9001 cataccatct ttaataatgt gatagtgttc atctacatca tcttgatcat cgttatactg
     9061 attgtaccct tctaaatcaa atacttctgt agtagttaca tctagtggga cttttactgg
     9121 tacaagagta cgtgcacaag gcattgtata cgttccagta atgtgaatat ccgcaacgac
     9181 ttctgttgac ttaatggtta actgaccttg gattgtaatt ggagataaat caattaaatc
     9241 taatgattct tttaaattgt caaaactcac cgtttgatca aattcaaatg gcttaccttg
     9301 atatttcctt aattgcgtaa ttgaccattt catatggctt cacctctaac aagcacaaaa
     9361 tttattttaa ctttctaaga acaagttgtc aagatttttt cttaacatat catgttctgt
     9421 tacaatatta ataatgaata ccgaaaggac gaattgcaaa tgaaaagcgt tggcttaatc
     9481 acagaatata atccctttca taatgggcat caatatcata ttaatcaatc taaaaaactt
     9541 acaaatgctg acgttactat tgcaataatg agtggtaact ttgtcatgcg tggcgaacca
     9601 gcaatctata ataagtttac tcgtgcaaaa atggcattat caacagctga tttagttatc
     9661 gaactaccag caactgccag tttatcatct ggcgatcatt ttgccgaact agcagttaaa
     9721 gtcgcagatt atatgagtgt cgatacaatt gcatttggta gtgaaaataa tgatatcaaa
     9781 acattaaagc aattagcaca cagcattaat gaaattgaac aatctgaatc cttttcacaa
     9841 aaagtaaaag aaggtaaaag ctaccctcgt atcattagcg aattattaga gcatcatgaa
     9901 gcactagcaa gtcctaacaa catacttggt attagttacc tgaaagcaat tgctaaaaat
     9961 gctaaaaaca tcaatgcaat ttctatcaaa cgagaaaatg ctcaacatca tgattcatta
    10021 attcaacacc atcagtttgc aagtggtaca tctattagaa catcaatcat tagtcaagat
    10081 gatcattggc atcatgtggt acctaaagat attcaacact tgtacgtaac acctcatata
    10141 acattaaatc aaatatttcc atatttgaaa tatcaaatca tagctatgac tacagattca
    10201 ttaaaaaata tttatactgt tactgaaggt tttgaaaatc gtttgaaatc taacatttat
    10261 gaagcaactg attttcatca ttttgtaaaa ttactaaaaa caaagcgtta tacatatacg
    10321 catatccaaa gactgttaat gaatgtatta ttaaacatta aacctacaga tgttacgagt
    10381 aacattcacg cagttaaagt attagcgatg aacgaccgtg gcagacaata tttaaagcat
    10441 ttaaaaacag cgttcccaga aagacaatat ataaccaata ttaacaaaag caatgcacat
    10501 tacttcacaa atgaaatcaa agcaacacac atatacaatg caataagcgg gcaacaacaa
    10561 acagatttca acacgcctgt catacagcaa tatcgttaac aaatgaaaac agtattttag
    10621 gattgtaaac catgataaac ctaaaatact gttattttta ttacttaaat ttcttcttca
    10681 atgccttttc aacataaggt ggaacgaatt cagaaatatc tgctcgataa gctgcaactt
    10741 ctttaacaat acttgaactt ataaatgaat aattagtact agacatcata tataacgttt
    10801 caatttcatt gttcaacttt ttattcattg aagttaagcg taattcatat tcaaaatcac
    10861 tgactgctct taaaccacgt atgattgttt tagctcctac ttgttcacaa taatcgacta
    10921 gtaaaccact aaattgatga accttgacat taggtaaatg tttaacagat tgttcaatta
    10981 aatccatacg ctcttctaaa ctaaacgtac cttctttttt actattttta agaacacaga
    11041 catgaatttc atcaaatcta tctgtacttc tctcaataat gtctaaatga ccataagtaa
    11101 tggggtcaaa actacccgga atgaccgcta ttgtatgttc catgctattc tcccttttct
    11161 aataacaatg tgtctgtcaa cccataatgg taacgtttaa tcatattaaa cggttgataa
    11221 tctatttctt catgattgct aaattcacaa acgatgatac cattttcttt caataaatta
    11281 aactctgaaa ttagttttaa agctttatca atgagacctt tattataagg tggatctaag
    11341 aaaatgacat caaattgaat atcacgtttt gacaatgctt ttaaagctct atctgcatta
    11401 tttttataaa cttcagattg tgcctctaaa tccaaattcg caagatttga tttaataact
    11461 tttacagctt taaaattttg atcaacaaag attaccttat ccatacctcg agagagtgct
    11521 tctattccaa gcgccccgct tcctgcaaat aaatctaaac ctatacctga cacatcatat
    11581 aaactattaa agataccttc tttaacttta tccatagttg gtctcgtatt acggccttcc
    11641 atactttcta aagctttact tttatgttta cctgcaatga cgcgcatgtt gttcacactt
    11701 ccaattcatt tagttattta atataattta ttgagaaaaa ggagaatgat aaaccaatga
    11761 aacaaacatt tattacactt ggtgaaggtc taacagattt gttcgaattc atgacgatga
    11821 ttgaatataa ccatcaacgt attgataaaa ttatctattt tcattcacca caagctgaaa
    11881 ataaaaagtc atctgtagca atcattatga accctacaac tggcaatcat ttccaagcat
    11941 tttatatcat gataaacgct attaaatatc catatccaga ttcaaataaa aagtttcaaa
    12001 tgataaatga ttgtgctgaa aaattcgaca taccaatttt aggtatcgat gtacagcccc
    12061 ctcaagcatt tcatgattta tcgttatatt ataattattt aattagtgtg ttaaggctcc
    12121 aaaaatggat accagaactt caataatatt aattatatat ttcgtgtttc tctttttcgt
    12181 aagttttctt taagtattta tatggtgagc catcaatgtg tctaacgtat ttaagttgca
    12241 ttagtttatg tacaatttga tcaacatctt gctcattcac atacataatt acaaatttac
    12301 gatctctatt tgaatgaacg atatgtccat attttcggat ttgtcgttca tgtttcatat
    12361 gttttaaata aactacaata ctagttcttg ggattaaatt catttaaacc tcactccatt
    12421 tattttcagc attaatcata
//
LOCUS       DAKTLH010000074        12406 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.074, whole genome shotgun sequence.
ACCESSION   DAKTLH010000074 DAKTLH010000000
VERSION     DAKTLH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12406
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.074"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            86..1915
                     /locus_tag="O0394_001676"
     CDS             86..1915
                     /locus_tag="O0394_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCY0499756.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSAKNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     LPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(2055..4133)
                     /locus_tag="O0394_001677"
     CDS             complement(2055..4133)
                     /locus_tag="O0394_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCY0499757.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
     gene            complement(4271..5680)
                     /locus_tag="O0394_001678"
     CDS             complement(4271..5680)
                     /locus_tag="O0394_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0499758.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
     gene            5966..6772
                     /locus_tag="O0394_001679"
     CDS             5966..6772
                     /locus_tag="O0394_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCY0499759.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTDWYDYSFAAQRFSLDELQKGKHYGATWQDKERISWGISDQNLSKIA
                     AEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVIDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(6835..7491)
                     /locus_tag="O0394_001680"
     CDS             complement(6835..7491)
                     /locus_tag="O0394_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCY0499760.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            7604..8077
                     /locus_tag="O0394_001681"
     CDS             7604..8077
                     /locus_tag="O0394_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCY0499761.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYMIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(8200..9411)
                     /locus_tag="O0394_001682"
     CDS             complement(8200..9411)
                     /locus_tag="O0394_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCY0499762.1"
                     /translation="MQSTKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(9593..10252)
                     /locus_tag="O0394_001683"
     CDS             complement(9593..10252)
                     /locus_tag="O0394_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499763.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
     gene            complement(10312..11454)
                     /locus_tag="O0394_001684"
     CDS             complement(10312..11454)
                     /locus_tag="O0394_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCY0499764.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIT
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            11722..12108
                     /gene="gtxA"
                     /locus_tag="O0394_001685"
     CDS             11722..12108
                     /gene="gtxA"
                     /locus_tag="O0394_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HCY0499765.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFLVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLIITIPITFILSKWILKDSN"
     gene            12242..12349
                     /locus_tag="O0394_001686"
     CDS             12242..12349
                     /locus_tag="O0394_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCY0499766.1"
                     /translation="MFNLLINIMTSAISGCLVAFFAHWLRTRNNKKGDK"
BASE COUNT         4132 a         1938 c         2242 g         4094 t
ORIGIN      
        1 aaaatattaa ttaactctct atactttttg gaaagatagg cgtatagtga tatccatcaa
       61 aaactataaa aaggaatagg agattatgtt caatcaattt aaaagactta ttatagggca
      121 acctaaaaaa aacagagaac taaaagacga aaagatatcg aagtttaaag ggttagcgat
      181 tctttcgtcg gatgcattat cttcagtggc ttacggtccc gaacaaatac tgattacact
      241 atcagtagtg ggtgcagttg caacttggta tactttaccg attgcaggtg ctgttctgat
      301 cttattagct gctttaatta tgtcttatag gcaaattatt tatgcatatc ctaaaggtgg
      361 cggcgcgtat atggtgtcca agaccaattt aggagaaaaa tggggattgc ttgcaggagg
      421 ctcattatta gtcgattaca tactgactgt cgctgttagt atatcatctg gtgctgatgc
      481 atttgttgct gcattcccaa gtttatatgg gcataaagta ctgattgcat gtttacttgt
      541 actttttatt ttaatattaa atttacgtgg tttaactgaa tctgccaccg tattatccta
      601 tccagtatat cttttcatta tagggttagt gatattaata tttataggta ctttccgtgt
      661 ggcgacagga gatattcaac cacatatgca tgcatcagta ggaactgcgg ttcctggagt
      721 aacattattc ttgctattaa aagcattttc atcaggggct tcatcgttaa cgggtgttga
      781 agctatttca aatgcggtaa ccaattttag agagccaagt gctaaaaatg ctgtgaaaac
      841 gttaattgct atgggttcaa ttttagcgtt tttattagtt ggtatcgttg gtttagcata
      901 tgtttatgga attttgccac aaacggaaac gacagtttta tcacaattag ctatgcaaat
      961 atttggtgat aatgctgcct tctattttgt acaggcaacg accgtgatga ttttggtatt
     1021 agcagccaac actggattta cagcttttcc gatgttagcg gcttcaatgt ctaaagataa
     1081 atatatgcct agaatgttta ctgttagagg tgatcgctta ggctactcta actcgattat
     1141 tattttaggt gtattagcca ttattttaat tattgtattt gatggtatga ctgaagattt
     1201 aattccattg tatgcggttg gtgtatttat tccatttaca ctagcacaat tcggtatggt
     1261 tattaaatgg attcatgaac gtcctaagaa ttggttaagt aagttgtctg tgaacttact
     1321 tggtggtatc gtgacattta ttgtttttat gattttactc ataactaaat ttagccaagt
     1381 atggccgata cttattttct tgccgttcgt ggtgattttc ttcttgaaaa ttaacaaaca
     1441 ttatcgagat atcgcagaac aattacgttc tgacattgat gttcttaatg tggacgtggt
     1501 agatcgtaat ttagcaattg tgccgataac aagtattaca acagcagttg ataagtctat
     1561 ttattacgca caaatgcttg cgaataatga tgtcatagca gtacatgtat catttggaga
     1621 tgaagatgaa aaagcattcc aagagaaatg gaaacgtcat ttcccagatg taagattagt
     1681 tattttacat tcggaatatc gcagtattat acgaccaatt tcacgtttta tcgataagat
     1741 taatcgtaaa gccaacgatc aaaactatat gattacagta gtgataccag aatttattac
     1801 taagaaacgt tggcataatt tattacacaa tcaaactagt ttgcgtatga aattgtattt
     1861 aatttatcaa aagaatgtta acgtatgtac aattccattt aaacttaaaa aatagattct
     1921 ataatcaaat cggattgatg aaaggttatt tacttttcat caatctgatt ttttgatgtt
     1981 gaatataaaa accccgtaca tatgagatga taatgacttt gtctcatatg tacgggggta
     2041 ttgttatgtc ttgcttaatg tttggactgt aaagacaaga caatttcgtt gtaattgata
     2101 ttttctcgaa tttgagctgc aacgatattg ttgattttat ttttgcgaat taattcatct
     2161 aaaagatgac gttgtaagta caatgcttcc aatttgtggt tatggaacat agcttgcggt
     2221 atgatttgat tgctgttttc ttgacgttct tttcgacgtt gtgcacgcca ctttaatgag
     2281 cgactcatat catagtaatg attaatgact aagctaacct caagtacatt tgtactattt
     2341 tgttcttctt ttaagcgtaa aataatattg tgattcacaa tacgcataat tttttgaaca
     2401 tctttgaatg aattttcaaa ttcttgcttc ttagattctt tatttaaggc tttgtagtgc
     2461 ttcatcgctt taagtctaag ccataacatc ttgaagaagt ttgcaaattt ttcaacaaat
     2521 gatgcagtac ggtgtgtctc tgttaattcg acaatattgc ggtaattatt aatatcctga
     2581 tatgttgcct tacctttatc aattaaacgc tcaagtgtag atgtttcgat tacttttgca
     2641 atatcttcta aacgtttgag ttcttttgta ttttgattat caggttctga atttaataaa
     2701 aataacaatt ctccatagta ttggtttaat actgggcgat aatttgtatc gtttttgtct
     2761 tttttacttt cgtttttaaa atgctggatc actttttgaa caatgaaaat tttggcagat
     2821 tgataagtca tacctttaaa agtagtatct tcggcagatg gtgtaattaa aggtaaaaca
     2881 atttgcgcta agattaaact aattaatacc atgaaagatg caataaacaa taaatcatta
     2941 cggtattcga atgcttgtcc ttttgtaatg ataaatggta atgtaagtgc cattgaaagt
     3001 gaaattgtac cgtgaatacc acacatggtc ataataaatg cgtaacgcac tcgagaaggt
     3061 ggtgtttcat gtgaatcatg ttcctcgtct agataagatt gtatattttt cgggaaataa
     3121 aaatctttgt accagaaata aacccaaaca aatcgacatg cataaattgc aatagcgatt
     3181 aatatagtga tcactattaa aaatgaaaag ttgtcaggct ctgtttggaa aatatctatg
     3241 actacagtag gaatcataaa acctaataca acaaatacaa aaccatttaa ggcataacta
     3301 aatgtgttcc aaatttgatg gtaattcatt tgtagttccg tttgtgctct aattaaacgg
     3361 tcgcgttcta aaccatggat tagacctgcg attacaactg caatgatacc tgaagcatga
     3421 acttcttctg ctaaaaagta tacgacaaaa ggagttaata attgaataaa agttaaggta
     3481 ttgttatctt ttaaaccttt attagcggtt aagtctatac gtattctaac gacaacgaat
     3541 ccaataattg caccaataag tacacctagt attgttgaaa tgataaattg ttcaacagct
     3601 tggaataatg aaaaggtacc agttactaat gcagtaacag caattttaaa tgaaatgata
     3661 cctgctgcat cattgagtaa agattcacct tctaaaatcg tcatagaacc tttaggtaat
     3721 aattttccgc gtgtaatagc agatactgct actgcatcag taggacataa aattgctgct
     3781 attgcaaaag cggctggcat tggtaaggca ggccaaatcc aatgtataaa atagccaaca
     3841 ccgactacag ttgcaaacac tagtgccatt gacattaata gtataggttt acgatattct
     3901 aataattttg ttcgagagac gtgggtacct tccacaaaaa gtagtggcgc gataacggca
     3961 aacataaata cttcagaatt gaattggaaa tcaacttgta ttggaataat gaaaataacg
     4021 acacctaatg caatttgaat aaaggcagta ggaatttgtg ggaatcgatt attgataacc
     4081 gaactaataa tcacagcaaa aataaaaatt aaaaatgctt ctaatagtgc catacaatac
     4141 tcctcaaaat tttaatagtt aatattttat cacttttaag gcataatgac atagatatat
     4201 tgataaaatg aagttatttt caaaaaaact ctagtatcgg ttgaactgat actagagcga
     4261 gatgtttaaa ttattgattg tcatatctga aatgaccgct gtcattttgt cgttgttcat
     4321 acgcgagctt ttcagcattc gttttgtatt ttttataaaa gaaaaataaa aatataaacc
     4381 agaatggcga aatataaata gctgctcttg tttcgtcact aaagaataat aaaatgaata
     4441 caaagaagaa gaacgctaga ataatgtaag caataggctt accaccaatc aacttaaatt
     4501 tactgttttt atgtgcctca ggatgctttt tcaaatacat tagataagcg acaataatca
     4561 ttgcccaaac aactaaaaac aacacagttg ataacgttgt aacgtatatg aatagttgaa
     4621 ttgcattagg gaaaatgtag tttaacaagg ctgctataag taatgcaatt gatgaaacta
     4681 acattgataa ataaggcacg ccatgactat tcgttttatt taaaacttta ggacctaacc
     4741 cttgttgtga cagtccgaat aagatacgac tattcgaata gataccacta tttgtagcag
     4801 aggccgcggc agttagcacg acaaagttaa cgacacctgc tgcaaatggt acgccgatta
     4861 atgtaaagag tttaacgaaa gggctactat ttggatcgat atcattccaa ggtatgactg
     4921 acataattac taatagacca ccgatataga ataataaaat acggataggt acattattaa
     4981 ttgctttcgg taaggttttt tcaggatctt tcgtttcacc agcagttaca cctataagtt
     5041 caataccaat gaatgaatat acagcaattt ggaatgacat taagaaacca aatgttccac
     5101 cagggaacat gccaccgtga ctgattaagt ttgtgaatga tgcatgtcca taatgtgttt
     5161 taaatgagaa aaagattaat acaagaccaa cgataaccat cgcaataatt gtgacaactt
     5221 taataatcga gaaccaaaac tccagttcac caaataatct ggcacctaat aagttgaagc
     5281 taatcaagat taaaacaata aatagcacgg taatccaatt tgggacttgt ggataccaaa
     5341 atgcaaagta ttgtcccatc gcagttaggt ctgacatact tgatacaatc caacataacc
     5401 agtaagtcca gccaatgaca aagccaccaa aagggcctaa atattcattt gcaatatcaa
     5461 caaatgaatt aaatcttgta ttgctcaaca acaattcgcc taatgcgcgc ataaaagcga
     5521 atagtacaac cccaataatc atgtatgtga ataacagtga tggaccagtt aaagatattg
     5581 tttgacctga tcctaggaat aacccagttc caattgcgcc cccaattgct attaattgga
     5641 tgtgtctgtt attcaactct ctatgcaatt ttcttgccat aattttcctc ctcaggataa
     5701 agcaaataaa aaagttgtgt gtaagcgttt tcttaattat aaatactaaa gaaatccatt
     5761 taataaagca attatttttt taaatataag aaaaaacatt tgataggtaa gggtatgata
     5821 actaaaactt atcgattttt tgaaaagata taagttttta tgcaaaaata taaaataatc
     5881 atctaaatga agcagttaac tatatatatt agataagcta tggcataata aataacattt
     5941 taatttgagt aaagaaggta agggtatgaa gatagggaca atcgccgatt tacatattga
     6001 tcgtcataat aaaaaaacat ccgaagatta cttagaggca ttggttgaaa tagttaagta
     6061 taagaaattg gatatattgt tgattgcggg ggatatctca aatcattatc aattgacgca
     6121 ccaatttata acgcaattga caaaacaatt agatatacct gtgaaatttg tacctggaaa
     6181 ccatgattta tgggaagttg aaagtatgac tacgcaagac atttggaata attataagag
     6241 tatgtcacag tgcttggtag gaaaaccatt tatagtaaat gaagaatggg caatcatagg
     6301 acatactgac tggtatgatt atagctttgc agcacaacga ttttcattag atgagttaca
     6361 aaaaggaaaa cattatggtg cgacttggca agataaagaa cgaatatctt ggggcatatc
     6421 agatcaaaat ttatctaaaa tagcggctga acaagtgaag aaagatatat tagaagtagg
     6481 aaatagacga gtgattttag tcacacatgt tgtgacgcac cctgatttca ttgttcctat
     6541 gccgcatcgt atattcgatt tttataatgc atttattggg acaagtgatt tcaatccttt
     6601 gtatgcgatg ttcgatatac catatagtat tatgggccat gttcattttc gtaaaagtgt
     6661 gatagatgat ggcagatgtt atctctgtcc gtgtctaggc tatccaagac aatggcgttc
     6721 agaagatatt taccaggaaa taaatgagac gatacaaata atagaaattt aaaatgcgca
     6781 aacctgaccc agtttgcgca ttttatgttt tacacacgcg agtaatgtgt ttacttacgt
     6841 gtgtttattt tgttgctgat tttcaattgt atatgaatgt ggttgcacat aaatgcactt
     6901 tcttcctggt gaattaaagc tgtattccat tttctcttta cgaattttaa taatttgttt
     6961 gcgatttgga atgatggcag gtaaaactag gccacgacga atatgactcc aaagtggatc
     7021 gatataatag taatagtaat catcgtaccc aatgagtaac gttacgtgaa tatttgaaac
     7081 ttgtttcgct ggcttgttat caaagcggaa aacacgtagt aatggtttag aaccaagatt
     7141 agtatggtat attaacacag gttgaccttg atcgataata cctttaagat cttctaacga
     7201 tttaccagtg ccgtctacga tattaggatt gtatttttgt aaaaatggta catatgcttc
     7261 tggaaatatc gtttgatgat aattgccaag cttaatgaat aagtgatgtc caacataacc
     7321 tttatgtgga ttgttcggat gtgtcggcca atgtctcata atttctgtag cagggatatg
     7381 ttggttgttg tattgcaaca tcatggctgc ggaaacacct tcacacccca tgaccatagg
     7441 gataggaaat agctgactga taggtttaac tggtaatatt tttcggttca taatatagtc
     7501 ctcgcattga ttcaataaat atttaatata attatatagc gtcaatgcaa aatgtcctaa
     7561 acatatgttt tacatgagtg aataaaatta atggagtgat aaaatggaat atcaattaca
     7621 acaacttgcg tcgttaacgt tagtaggtat taaagaaacg tatgaaaatg gacgacaggc
     7681 tcagcaacat atagcagggt tttggcaaag atgttatcaa gagggagtaa ttgcggattt
     7741 acagttaaaa aataatggtg atttagccgg gatacttggc ttatgtatac ctgaattaga
     7801 cggtaagatg tcatatatga ttgcagttac cggagataat agtgctgata ttgaaaaata
     7861 tgatgtcata acattagcaa gttcaaagta tatggtattt gaagcacagg gcgcagtacc
     7921 taaagcagtt caacaaaaaa tggaagaggt tcatcactac atacatcaat atcaagcaga
     7981 tacggtaaaa tcagcaccat tttttgagtt gtatcaggat ggtgatacta caagtgaaaa
     8041 atatattaca gaaatttgga tgcctgttaa agggtgattg aatagaaatg tccttgctat
     8101 gaaggcgtaa ttttagttga agattcgatc tcataattca taaagaagaa ccctgacaaa
     8161 gatagatatc tttgctaggg ttctaattat gttgaagtgt tatgcgacat gttgcttttt
     8221 aatcatttta aagtaaatga tttgtagact gactaataga atggctgtaa tgaagataat
     8281 aatcatatat ggtgatgtat taaattcgcc ttttaagcca actaatggtg caacagcgcc
     8341 acctaagata aattggaata agccaagtaa acttgatgcg ttaccactgc cacctgttcg
     8401 ttcttccata gccattgtga aaccaagcgg tccaattgac gtcacaggac acacatttaa
     8461 gaagaatgcg atgagtaaga cccaaagtgg taaatggaat gtaagtgtca ggataattaa
     8521 agcaacacct accacttgta taatagttaa aatgattaat aatatgtggc gatgtaattt
     8581 ttctactaat aaagcaacga cttgactgac aatgattaaa cctacaccgt taacagcaaa
     8641 catgatacta aattgttggg gtgtcatatt atacaatttt tgagtaataa atggcgatgc
     8701 agatgaataa ctaaatagca ttacataagt taaaccttgt aatagcattg gaataataaa
     8761 tgctggtttt ttcaaaagac ttccaaaatc tttaatgacg ctactaaaat tcacctgctt
     8821 taattcatct ttagatgttt taggtaattg agaagcgacg ccaattaaaa tgatgagtgc
     8881 cacaatagtt aaaattgtga aaatagaacg ccatgttgct acggataaag ctaatccacc
     8941 ggctaatggt gcaagaatag tgatgatgcc attaactacc attaaagatg ctaaaaattt
     9001 agcgagtgcg ttgccactaa atttatcacc agcagaggct tttgcaatca cgatgacgcc
     9061 accaccagtt aaaccttgaa taaatcgtaa agttaagaat aatggcaatt gatcaacaaa
     9121 catagacaaa cctgatacca aaatgaaaat gatcaaaatg gatacagcaa tccgtttgcg
     9181 accaaaagca tcggataaag gtccaaagat aaattggcct agtgcaagac caatcattgt
     9241 gaatgataat gttaattgaa tttctgatgt agtagaacca aaatcacctt gcactttagg
     9301 taatgatggt acgtacatat ctatagttag tgggccaaaa gcggtcatga cgcctaacgt
     9361 aattagcaat aaaaatgaaa aatgcttcgt tttggttttc gttgattgca tgatcagact
     9421 cctttaaatg atataattat ataaattata tacgttttgt gtgtatttgt atgtacttag
     9481 aatacaagtt tatataaaaa atccctatac tcttaatgca tcatcacgaa taacgtagtg
     9541 atacagataa agtataggga attaatgttt atcatcaaag caggtgtaat aattaattag
     9601 cgccaccatt gtcatcttta aataagcttt ctaatttaga tttaatactg tcgaattttg
     9661 tattaatatc atttaacatg ctttcaattt taccttggct tgcattgttt ttaagtgcat
     9721 tatttgcctc tgtcaatttg ttgatttggt cttgataaaa ttgagttagc ttagaatcag
     9781 ctttgttttg ttcttgctgc ttcaagttgt cgatttgatt ttgtaaattt ttaataatgt
     9841 catcagtttt ttggttgttt ttaatatttt ctttagcatt atctacttgc tggttaattt
     9901 ggccgtcttg attttcaact tgttttttat aattttgaga gactttaggg tcattactaa
     9961 ctttatcgct atcattgtta agtaaggcgc gtgtgacaaa aatagcgaca ataattaaga
    10021 gaatggcaat aatgatatat aaccattttt ttgaacggcg ggatttctcg ttttcttctt
    10081 caagaatttg ttctcttcga taatctctac cattatagta tcgttccggg tgttcttcag
    10141 gatcgccttt agcatattgt tgtctatacg atgcgtcttc agattgacga cgatgatttt
    10201 ggttttgatt gtcgtattga tatgaatcac ggtatttatc agttcttttc atgggtttca
    10261 cctcattcaa gttttaactg caaataataa agctgatgat ttactttgac attattgtaa
    10321 tttcagaata cgagcaatgt cagttgcagt gtttaataaa ttttgttcgc tattttgtag
    10381 gacatcttct aaagtgctag gtgaagagat tatagaatag gcactatcaa taccgaaatc
    10441 gtaaacatgt tgataatttt cgcctagact gccacaaatc gcgatgacag gaatatgata
    10501 ttgttttgca gctaacgcaa cgcctacggg tgttttacca aagatggtct gataatccat
    10561 gcgtccttct ccagtaataa cgaggtctgc atctttaatt ctttgatgaa aatctgtaat
    10621 gtcaaagacg acatcaatac cttttgttaa agttgtctca caaaacgcta ataatgctgc
    10681 gcccataccg ccagctgcac cagaacctgg tatttgatta acggactttc ctgtgcacat
    10741 ttttatctta tcatgataat gcgacattgc gaaatccaac tttggtatca tctttgcatc
    10801 agcgcctttt tgaggaccat aaatataggt agcaccattt tcacccaata aaggatttga
    10861 aacatcacag gccactttaa aggtcacctc ttttaatcgc gaatctagat tggttatatc
    10921 gatttgtgca atgtgagcaa gattagcacc attcatttgt aataagtccc cgtttacatc
    10981 agtaaacttt acgcctagtg cacttagcat acctgtacca ccatcatttg ttgcactgcc
    11041 accaatccct aaaataatgg tcttagcacc atgatttaat gcatctttaa ttagttcacc
    11101 ggtaccatat gatgatgtgt ataaaggatt acgttcctct ttttctaata aatccaaacc
    11161 tgacgctgcc gccatttcaa taattgcaat ttgttgttcg tctgcgcgtg cataacatgc
    11221 ttcaataggt cgcattaaag ggtcattaac gatgactgta tacttagtgg cacctgttgc
    11281 atgcattaaa gcatctgtgg taccttcacc accatcagcc atcgttatga tatcataatg
    11341 aagggtattc ccataaacat tagtaaaagc ctgttttata atattgccaa cttgctgtgc
    11401 ggtcatactt tccttaaatg agtcaggagc gatgacaatt ttttgtaatt gcatagtaga
    11461 catcctctca ttactgttgt cgctcttatt gtatataaaa ttactagtga actaaagtaa
    11521 tctctatagt gctaatgagt cacgcgataa atcagttaca ttataatggc gtataaggat
    11581 gaggggaaag agactaaatt tgctgtgaaa gggtataaag attgaaataa gcatatttga
    11641 agtgaagcgg attggctcgc atattcgagt atgttcgaga acgtagttat gcatatattt
    11701 tgatgttgag gagttacaca tatgaaacta acacaaacgc atgctgaaat tttaaaattt
    11761 atcattgttg gcggcattaa tacgttaaat tattatgttg tctatttatt gctgttaaaa
    11821 ttattacaca ttgaatatat gattagtcat attaccggat ttctagtcgc ttttgtgatt
    11881 tcatattatt tgaattgtta ttttgtttac agggtaaaac ctacttggag aaaattcatt
    11941 agttttccaa ttacgcagat tgtcaacgta agcttacaaa cagttttatt atatgtattt
    12001 gtatcctggc taaatttgcc agcagaaatt gcaccatttg ctggtttaat tattacgata
    12061 cccatcacat ttatattatc taaatggatt ttaaaagata gcaattaaag tagcgtaaac
    12121 cactaaaaaa aactttactt aagccttaat attgcgtgaa atataataaa taaatgatat
    12181 aatatgatgg tcgcctaaca tctcgggcgt tactttaacg cagagaggag gtgtatgacg
    12241 gatgttcaat ttattaatta acatcatgac ttcagctata agcggctgtc ttgttgcgtt
    12301 ttttgcacat tggttacgaa cgcgcaacaa taaaaaaggt gacaaataac caacacctaa
    12361 ataaatcccc tcactatggc agtagtgagg ggatttggtg tattta
//
LOCUS       DAKTLH010000075        11918 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.075, whole genome shotgun sequence.
ACCESSION   DAKTLH010000075 DAKTLH010000000
VERSION     DAKTLH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11918
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.075"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..179)
                     /locus_tag="O0394_001687"
     rRNA            complement(<1..179)
                     /locus_tag="O0394_001687"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(298..371)
                     /locus_tag="O0394_001688"
     tRNA            complement(298..371)
                     /locus_tag="O0394_001688"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(337..339),aa:Gly,seq:tcc)
     gene            complement(394..476)
                     /locus_tag="O0394_001689"
     tRNA            complement(394..476)
                     /locus_tag="O0394_001689"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(441..443),aa:Leu,seq:gag)
     gene            complement(1123..1578)
                     /locus_tag="O0394_001690"
     CDS             complement(1123..1578)
                     /locus_tag="O0394_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCY0499767.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
     gene            complement(1571..3721)
                     /locus_tag="O0394_001691"
     CDS             complement(1571..3721)
                     /locus_tag="O0394_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCY0499768.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLQEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIYSIDKNKDKVSLTMIDPHE"
     gene            complement(4155..4925)
                     /gene="sigB"
                     /locus_tag="O0394_001692"
     CDS             complement(4155..4925)
                     /gene="sigB"
                     /locus_tag="O0394_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HCY0499769.1"
                     /translation="MAKESKSANEISPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHQ"
     gene            complement(4900..5379)
                     /gene="rsbW"
                     /locus_tag="O0394_001693"
     CDS             complement(4900..5379)
                     /gene="rsbW"
                     /locus_tag="O0394_001693"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HCY0499770.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            complement(5381..5707)
                     /locus_tag="O0394_001694"
     CDS             complement(5381..5707)
                     /locus_tag="O0394_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HCY0499771.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            complement(5826..6827)
                     /locus_tag="O0394_001695"
     CDS             complement(5826..6827)
                     /locus_tag="O0394_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HCY0499772.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTILIIKRVN"
     gene            complement(7177..7539)
                     /locus_tag="O0394_001696"
     CDS             complement(7177..7539)
                     /locus_tag="O0394_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCY0499773.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
     gene            complement(7536..7706)
                     /gene="mazE"
                     /locus_tag="O0394_001697"
     CDS             complement(7536..7706)
                     /gene="mazE"
                     /locus_tag="O0394_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCY0499774.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            complement(7791..8939)
                     /gene="alr"
                     /locus_tag="O0394_001698"
     CDS             complement(7791..8939)
                     /gene="alr"
                     /locus_tag="O0394_001698"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCY0499775.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            complement(9005..9364)
                     /locus_tag="O0394_001699"
     CDS             complement(9005..9364)
                     /locus_tag="O0394_001699"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCY0499776.1"
                     /translation="MIHGIGVDLIEIDRIQALYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            complement(9368..9859)
                     /locus_tag="O0394_001700"
     CDS             complement(9368..9859)
                     /locus_tag="O0394_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCY0499777.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVLFWIPIALVLLIAFNILNWLFWEI
                     LDNHLSIVSSVIIILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            complement(9846..11427)
                     /locus_tag="O0394_001701"
                     /pseudo
     CDS             complement(9846..11427)
                     /locus_tag="O0394_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
     gene            complement(11420..11899)
                     /locus_tag="O0394_001702"
     CDS             complement(11420..11899)
                     /locus_tag="O0394_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499778.1"
                     /translation="MSDYTKLTKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLFIKPLWQYHCRFYKVDQLSVQYRTSFFIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEERTMSNLRGVESDV"
BASE COUNT         3684 a         2291 c         1569 g         4374 t
ORIGIN      
        1 atccggtatt agctccggtt tcccgaagtt atcccagtct tataggtagg ttatccacgt
       61 gttactcacc cgtccgccgc taacatcaga gaagcaagct tctcgtccgt tcgctcgact
      121 tgcatgtatt aggcacgccg ccagcgttca tcctgagcca ggatcaaact ctccataaaa
      181 attatgatgt ttgattagct cataaatact aaataatgtt tgtaacttat agttacgttt
      241 ttggaattaa cgttgacata ttgtcattca gttttcaatg ttcattaaaa ttatctatgg
      301 agcgggtgat gggaatcgaa cccacaacat cagcttggaa ggctgaggtt ttgccattaa
      361 actacacccg cttatttaaa ttaagaattt tgatgcggcc gagaggactt gaacctccac
      421 gggatttctc ccactaggcc ctcaacctag cgcgtctgcc attccgccac gaccgcggat
      481 ataggcattt aaaacatctt cgtttttcag aacgttattt attatattat gtttaaaaat
      541 atttgtcaat aacttttaat cgttttttta cattaaatta atttgtgtta tcagttgtta
      601 acacaacgct atttataata aaacatttag aacttattgt caacatattt tataaattaa
      661 atttagaatt gactgtcttt caaatcaaag tttatttaat ttgttctttt ttaaggaacg
      721 tgtttaatca tactaactat aagctgtaca gtcaatagaa aagttcaatc aaaatgtcat
      781 gaaaaatgta taattatctt ttgtgctact ttaaagcttt aattgggtaa agtttatata
      841 ttaattatta ctaaaatacg ccaagatttt taatctaatc attttaattt catcctatca
      901 agccacaatt tatgccatta cctatatcat aagtagaaaa gccgagattg cgaagtatta
      961 aattacgaca atctcggctt taaacatttt ataaacacaa tttcttctta ttcaattact
     1021 atgtttacca atcaaaataa ctcactgata aattctatat aaattccata caactcataa
     1081 ataatcttac atatacacga attagctcta tttgtattgt acttatttca attgtctttt
     1141 cattcttaat tttccattgc aatgtccgca acgcatacga ttcgtatcaa ctttacggat
     1201 acgtatatat tttgcatgac atttagtgca ataatattca taatttgccc gttgttgata
     1261 gctttcaatg ctgttacaaa atctaggcgc tccaacctgt tggctcaatc gtttaaaatc
     1321 ttgatcttta tgttgataac ctttaccagc aatatgcaag tgataatgac acaattcgtg
     1381 cagtataatt tttacaacag catcttctcc ataatgctca tattgttttg gattaatttc
     1441 aatatcatgg gactttaaaa gataacgtcc gcctgttgta cgtaaccttt tattaaaata
     1501 tgcacaatgt cgaaacgtac gtccaaattt ttcttccgaa agattctcaa ccattcgctg
     1561 aagtttgtca ttattcatgt ggatcaatca tcgttaatga tactttgtct ttatttttgt
     1621 caatactgta aatccaaacg tcaacgatat caccaacact gacaatatcc attggatttt
     1681 ttacgaactt cttagaaagt ttcgaaacat ggacaagtcc atcttgttta acacctatat
     1741 ctacaaatgc tccaaaatct actacatttc taactgtacc actcaatttc attccttctt
     1801 gtaaatcttc aattgatagc acatcggatt taaggattgg tgtttcaaac tcgtcccttg
     1861 gatctcgatt aggtgctttc aaggatttaa taatatcctc taatgtaggt acaccgactt
     1921 gtaattcaat cgccagttca tccatatcca atgaatttaa tttagctttt aatgcatcac
     1981 tacctaaatc attgccacca aatccgagtt tatctaataa ctgatacgtt actttatagc
     2041 tttcgggatg aatcgatgta ttatccaatg gctctgaacc gtctactatt cttaaaaatc
     2101 ctatactctg ctcaaaagtt ttagcaccca aacgcttaat cttgcttaac tctttattat
     2161 gctttattgc gccattttct tctctatatg caattatatt tttagcaatt tgcgaactta
     2221 gaccagacac atattgtaat aatgatgatg atgctgtatt gacatcaaca cctacttggt
     2281 taactgctgt ttcaacaacg aaagttaatg cattttctag tgctttttga ttaacatcat
     2341 gctggtattg tccgacacca atagatttag gatcgatttt aactaattca cttaatggat
     2401 cttgaacgcg tcgtccaatt gatacggcac ttctttcttc cacttgaaaa tcaggaaatt
     2461 catctctagc aatttctgat gctgagtata ctgaagcgcc cgcttcattg acaatgatga
     2521 attgtactgg caactgatgc tttttaatta aatctgcaac aaattgttct gtttcacgac
     2581 ttgcagtacc attgccaatt gcaattaatt gcacatcata cgctttaacc atttgtacaa
     2641 aatccttctc tgctgcctct tttttagaaa ctggtggatg cggataaatc acaccttttg
     2701 ctataaaagt accgaatggg ttaatgactg ctaatttaca acctgttcta aatgctggat
     2761 ctacgcctaa tatttgttta cctttcattg gaggttgcag taatagattt cttaagtttt
     2821 cactaaaaac atctattgca tgattttcag ctttttcagt taaatcagca tggatttcac
     2881 gctctatcga agggacaatt aagcgtttca agctatcttt aatcgcctct aaaatataac
     2941 tgcgatttac attattatga ttgatttctt gacgtgcaat gaaatcctct actgatgtcg
     3001 tatcgaattc aaactttaca gataatactt tctctttttc accacgatta acagctaaaa
     3061 ctctatgatt agcaatgcgt ttaattggct cactatatgc atagtacatt tcaaaaatac
     3121 ctttttcatc ttcagcattt ttctttttag atgtagttaa cacaccttga tgatacatat
     3181 cttttaaaat ttttgttcta tatttaggat tatctgaaat ttgttccgca ataatatctt
     3241 gtgcaccttt gatagcatct tcaaccgatt gcacttcttc atttataaat tgttgtgctt
     3301 tttcttcaat tgagacttca tgtttacgtg ccttcatcca tatcgctaat ggctctaacc
     3361 ctttacgttt cgcctcagtt gcccttgtct ttttcttttg tttaaaaggc ctatataggt
     3421 cttcaacacg ttgtaatttg ttctgtttta aaatatcctt ctttaattcc tcagtaagta
     3481 atccttgctg ttctatattt ttgataactt cttctttacg tttttgtaaa ttgaccatat
     3541 attggtattc gtcatcaatt tgctttattt gaacttcatc tagtccacca gtttgctctt
     3601 ttcgatacct cgcaataaat ggtactgtat ttttttcttc tagcagtgtt aatactgctt
     3661 caatttgttt tttactaaat tgatatttct ctatgattga attaatcaat tgattgtcca
     3721 tttacttaac ccacactttt tcttaaattt actcatttat tttaacatat tctaaaatac
     3781 ttctattaag gtatgatact taatgtaatt ttcacttcca aaacatttaa aacgaatgat
     3841 taggtcatac tatattttaa aacgcactaa gattattgaa aatgacacac catcatttct
     3901 aaatttcata atctaagact acttttttta ttctaatagc aacccaccta tttaaaacga
     3961 caattagata tcttcttcac ttaaattatt aatactatat cgcgacattt atgtggatac
     4021 acattaaact acactaataa tgtattaatt cgaaaatctt aattaaatta aaaatacccc
     4081 tcgatttcaa tttaataatc aagggataat tatatggatt tttcattata aaacgtatca
     4141 ttaataaaca aattctattg atgtgctgct tcttgtaatt tcttaattgc cgttctctga
     4201 agtcgtgata catgcatttg acttaaaccg atacgctcac ctgtctcttt ttgactcaat
     4261 ccttcaataa acgtacattg tatgatttct cgttcgcgat cagataatat aggtaatatt
     4321 ttttctaaaa tcatacgttt ttcagttaag tcataatggt catcttgttg ccccataata
     4381 tctaatagcg taacagttga accatcttta tcagcttcaa tggaatgatc aacacttaac
     4441 gcattataac tttgtcccat ttccattgct tctaacactt cttcttctga gacttctaat
     4501 cgatcagcta tttcactgat agaaggtgaa cgctctaatt cagcggttag ttcatcgctc
     4561 acttttttga ttcttggccc aatttcttta atacgtctcg gaacatgtac actccaagtt
     4621 ttatctcgta gatatctttt gatttcaccg attacagtag gtactaaaaa ggcttcaaac
     4681 ttccgttcaa aggacatatc gaatctattt atggcaccta ttaaaccaac cataccaact
     4741 tgaactaaat cttcgtgatg tgattgtcct ttagaatatt tatatgccaa tgactcaatt
     4801 agtttttggt aatgtttaac taacttatcc tgtgcatctg tattcttatt ttcttggtgt
     4861 tctttaatcc attggttaat ttgctcaggt gaaatttcat tagctgattt cgactctttc
     4921 gccattattt cgcacctgct ctttttttat atacttagtc atactgattg tcacaccaga
     4981 ttctttatat actgtgactt catccattaa agattcgatt aaaaataaac ctaggccacc
     5041 ttcgcgtaaa aagtctatat tttcgtcttt atcgtaagga cctatttttg atttagttgt
     5101 ttcataatca aaactgtcac ctttatcaga aataacaatt ttaattttat cttctaaaat
     5161 ttcaaaatat atgttaataa tgcccacatt gttattttct ttgtatgcat gtttaactgc
     5221 atttgtcaca gcttcactaa ctgcaatctt ggcatcttca atatcatcat atgtagcacc
     5281 agctctcgaa aaaacgccag aaagtgttaa acgaattaaa cttacatact ctgccgatgc
     5341 tggcacgcgc atttcgataa aatcttcttt agattgcatg ttattcgacc tccgttcctt
     5401 cattaacatg cattaaatcc ttaagaccag taatttcaaa tagtctaccg atacgatctg
     5461 acacacctaa aatgtatagt tctttatcat tttggtttaa tgcttttaat gtacctacga
     5521 ataaacctaa acctgtcgaa tccatataac tcacattttc taaattaaca taaatatcac
     5581 gagttccatc ttgtctcata ggtgttaaaa cctcttctaa ttcaggcaca gtataaacat
     5641 ctaattctcc accgacttta acttcgtaaa atttatcttg agtggttgtt tctatattaa
     5701 gattcatttc attacactcc tacttattaa aaatatttat cttcatttta agcgctgtat
     5761 ccaccatatt tatatttcaa attattatat acccatcgaa ataatttcta atcttctttt
     5821 ttaaattaat ttactctttt tataatcaaa atagtcatat catctttttt atttgggttt
     5881 tgaagcttta aaattgcttc atagataatt tgaacaatat cttgtgggtg catatgttta
     5941 tgttttttaa tatattctaa aagtttttgt ttatctataa aggtaccttc actatttcta
     6001 gcttcagtca caccatccgt taaaatgata attaaatcat caaggtatat tggaatttct
     6061 tgttgttgat atcgtgtttg tgaactgatt cctaacactc tacctctaac tgaaatttct
     6121 tcaaattctt ctttttcagc gcgataaata tatccaggct catgaccagc tgaactacaa
     6181 tacaataaat ggttcatttc ttcatataaa ccataaaaca ttgtgacgaa catattttga
     6241 ttaatattct tttcaacaac acgatttaaa cgttttaaac catcactcgg taattgtgag
     6301 tgtccataag aatccatgcc aaactttatc atactcattg ctaaagcagc tggtatacct
     6361 tttccaatga catctgcaac agcaaagctc attgtgccat cgttatggtc aattaaatta
     6421 aaataatctc cacttacttt ttgtgccgcc actgaaataa cgccaatttg aatactatca
     6481 aattgtggaa tatctgtttt aagcattgtt tgttgtaagc tagaagctaa gtctatctct
     6541 ttatcgtgaa cttgaagttt atctaccaat ctttgataat ctcgataact ataaccaaag
     6601 cctttaacga tttcttgtaa gacatctaat gtcttgaaca catcttcacg cgttaagttt
     6661 aacgtcaata tatagttttt gtgaatatcg acaatgtctt caggcaagac gtccttacga
     6721 atcacttcgt cagtgtattt ctcacacttt tttatcaatt ctactttatc ttggcacgtt
     6781 aaactttcat caattaaacc cttataatgt tgcttaaatt cttccacgat tagttgcctc
     6841 ctcacttccc ttacaaagca aatgcttaag caaggataat tatgattcac ttactaacat
     6901 ttatttaatt gcacttatca attttgtcgt ttgcaatttt gctgtagatg atacttttaa
     6961 gctaaaaaaa actttaggat ggaataccta aagcctaaaa aacttaagac atccttttat
     7021 aataaaaaat atgaatatca taactggtat ttgctaaaat ctttgtttat aagaaaattc
     7081 ttcgtacaga atttagaaat agttataatt attataaagt tattgtcttt tatatcaata
     7141 ttttatttat ctctgaaaaa tacatataat agacgcctaa tttttctggt gagctactgc
     7201 attcagccct aaactaatca ttagtgcatt atctacttct ttcattttat catcggataa
     7261 gtacgtcagt ttttctttca atcgtttttt atcaagtgta cgaatttgtt ctaataatat
     7321 aactgagtct ttatccaact tatacttttt cttttcaatc tctacatgtg tcggtatttt
     7381 cgctttatta atcctaccag ttattgccgc aacaataact gtaggactat atttattacc
     7441 agtatcattt tgaattatga ctacaggtct gactccccct tgttcagatc cctgtactgg
     7501 tgataaatct gctaaataaa catctcctcg tctaatcatt cattcgttga attagaagat
     7561 aaatatgttt cgttgcaatc gcatgcttca cactctatcg gaaaagcttc gttcgctagg
     7621 gagagattta aatcagccat ttgtgaatat ccttctttta aagattgttc taagctatga
     7681 cttctatttt gactaaaaga taacatatga gaacctccaa ttgattccaa aagttattaa
     7741 atgaccttta attactctaa tgataacaaa ttttatatgc actgaccata ttattttaac
     7801 aattcatttg ttacaaaacg ttgatcacca tcatgataga ttcgcggcaa acgtctcgac
     7861 aagttacaaa gcacttcata attaatagta tgttgctttt cagctaccac ctctaccgac
     7921 tgtggacttt ctctatgatt atctattaaa atcaccgaat ctccagcttt aacttgatct
     7981 ggcactttaa caattgtctg atccatacat acgcgaccaa taacttcgca ttgatgacca
     8041 tttacattta caaagctacc ttgcattatg cgtaaatagc catctgcata tccaataggt
     8101 aacaatgcta ttgtagttgg gtcagtagct gtataagttg caccataact tacagactca
     8161 cccgcttgta gcgtctttgt ttgaactaca ttagcaatta attgcacact tggtttaagg
     8221 tgtactttaa ctttttgctg tacatactct gatggataat atccataaag ggaaattcct
     8281 ggtcttattg cattacagaa ttggcaatcc attaatagag agcctgctga gttctgacaa
     8341 tgtatatatt caggtttaat tgcttcattg accatatctt taaaacgttg atattgttca
     8401 gttgtcatat ctcctggttc gtcagcacag gcaaagtgtg taaacacgcc ttcaaataca
     8461 agttgctcat attgttgaat gatttcaatc acttcttgat acgttttagt atctttaata
     8521 cctaaacgtc ccattcctgt atctaattta atgtgcaacc ataacttttt ctcttgctca
     8581 ccagaaatgt ttttaattgc ttctttcaac cactgtttag acggaaccgt taaggcaact
     8641 cggtgttgta tcgctttatc aatatcttta gctggtaaca cacctaagac taaaatttta
     8701 gcagtaatcc catgcattct aagttctatc gcttcatcta acgttgctac agcaaaaaat
     8761 gtggcgccat tttccattaa atgacgtgct actttaacac tacctagtcc ataggcattg
     8821 gctttaacga cagccatcac tgttttattt ggatgcaatg tactgaatac tttgaaattt
     8881 gatgcaacag cgtttaaatc tacattcata tacgcagatc tataatattt atccgacata
     8941 ttacttcctc ctgtaattcc cacacgtttt aaaactagat cttaattatc attgtataac
     9001 aaatttaaaa tgctgacttt tctaaaacaa cttggctcat cgcataatgc tcagtgtgtg
     9061 agatactaac atgtacgata aacccttcgt aatcaatctt tggtttgcca agttcgtcgt
     9121 tgtaacagtc tatatcgtta aaagctacat gttttcctaa gcctgtgcct aatgctttac
     9181 tgaacgcttc ttttgtagca aacctgccag ctaaaaattc aattttacgt tgctcatgtg
     9241 tgaaattgtt gaatttgtgc tgttcatttt tagttaaaat ccgctcaacc aattttggtt
     9301 gcttactata taacgcttgt attcgatcga tttcgattaa atctacacca attccatgta
     9361 tcattgatta ttcctcattt ttattgtttt ctaaatgtct ctcgttattt aaccatcgtt
     9421 tcaatctacg tgcttcactt ctatctatta attcaatttc agcttgacca cccgctgtat
     9481 aaagggtcaa agttgctaat cgatagcgtc tcattatagg accaacatcg atatcaatat
     9541 tttgaatacg aaaatatggt attacctttt catccaaaaa taaaatgccg tttcgtacac
     9601 gcaaatggtg tttttcaaat gcatatctgc agtgcttata tcgatagact ggcgctataa
     9661 caagcgtgaa aacagcaaca agtaatatta taatcacact actaacaatg gataaatggt
     9721 tatctaaaat ctcccaaaat agccagttca aaatattaaa tgcgattaaa agtacaagcg
     9781 ctatgggtat ccaaaacagc acagcactta acctcatcac ttttttagcg tgtggtgaca
     9841 taaaattata atccctcatc attttcacct cttaaatacc atgatttcaa tttatttgca
     9901 tcttcacttc ttgcgtattt taagttaatc gattgggctc ccacaccttt agcaacaata
     9961 aagctaaaat tatttaaatt gttgcgttta agtaatgtat gttgccaagt gtcaaatcct
    10021 ataatgtgat gcgctttaaa ataataaata tttcgtttca atagctcgaa attctggata
    10081 gtaatttgtt cttctgtcat tttaaaaccc gcatgtttga cataaagata tcctttgatc
    10141 acaaataaac caataatgac tattgttata atcgtaaata acaataataa ttgattccaa
    10201 aaataacagc ctatacctgc catagctatg acaataatac taggtattaa aaagtgtctg
    10261 tggaaacctg acaaaggcat accttcatta acttgttgat aagataaatc tggtactaaa
    10321 ttctggataa tttgatatgc tttgtctcgt ttaataaacg gcaatatcgg cacactacct
    10381 gaatcattgt catcatcttc gattttttta ttttcaaaat cacttgtgat gataaaatgt
    10441 attgctgtgt agccaaataa tcttcgtata tatgactgac gttcaacaac agcttgcaat
    10501 cgtttcgttg gtacagttac tttcttcttg ttaaagagac catattcaat tttaagttgc
    10561 tcattttcaa gcatcaccga aaagccataa aatcttatca ttgttataat cgttccaata
    10621 atatatgcca ctattaatac tagtaaaatg atgattaata ctgaaatact tacaatttga
    10681 acccattgac taatttcatg atttagcttc gaccatggga tcaactctct tacagccccg
    10741 taaatcggta ctaaagctgc taacgttaca ccaatggcgc cactggtcat tgccataaat
    10801 agtgattctt taaaattcat ctgatatata ggaatgcgtt tatttttctg attaagcata
    10861 ctatcagtgt tctgcacttc atctaagcga ccttctgcga tgtcttccac attaccttca
    10921 atgtcatgat tacagttgtc attcttctca gcactagact tttgcgccac ttctgtcttc
    10981 aactctgttt gcaattgatc aatatatcgt tcaagatatt caccttgttt tttcgaaata
    11041 acacttaaga caaccatcac ttggtgtttt gatttgtaaa tccacaccac caataatttg
    11101 attgacgacc ccttgtgtca tatcaacgga ctgaatacgt tttatattta attctttacg
    11161 ctttttatta aaaatacctg ttgttaaaat gaaataatta tcctcaatcc aatatcgcgt
    11221 gttcataatt ccgacaattt gagaaatgta tgatattaaa aagaatacaa atacaatacc
    11281 tatccataaa tatgattcgg gattcgtata atcaaaatct ttcaattgaa agataatgaa
    11341 aataaaaaag acgactatgt tttgtttgat agcattgatt atgccattaa aatatgaaat
    11401 cggatgtaat ttttgaggtt cagacatcac tttcaacccc tctcaaattc gacatagttc
    11461 tctcttcgat tattttaaca tcgtcatgag acatcatcgg taaataaata gtatgacctg
    11521 cagtcataaa tccaacttta tacaaattaa gcactttact aattggatta gatttaatcg
    11581 acaagtattg taaacgttca attcgactcg tttcttcttt atatataaaa aatgatgtac
    11641 gatattgtac acttagttga tcaactttat aaaagcgaca atgatattgc cataaaggct
    11701 taataaataa ttttaatgta ctcagagcac ctaaaaccaa caaaatataa agtaagtaat
    11761 gtggccattc aaatcttaac catataaaat aaaaaatgac atacacagct acactcaata
    11821 taaattctaa gccattcgta atgtagtaat acaacaatgc tgacttagga ctcttagtca
    11881 acttagtata atctgacata tacccctctc cccaaata
//
LOCUS       DAKTLH010000076        11852 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.076, whole genome shotgun sequence.
ACCESSION   DAKTLH010000076 DAKTLH010000000
VERSION     DAKTLH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11852
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.076"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(164..1168)
                     /locus_tag="O0394_001703"
     CDS             complement(164..1168)
                     /locus_tag="O0394_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCY0499779.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
     gene            complement(1152..1862)
                     /locus_tag="O0394_001704"
     CDS             complement(1152..1862)
                     /locus_tag="O0394_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HCY0499780.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            complement(1950..2156)
                     /locus_tag="O0394_001705"
     CDS             complement(1950..2156)
                     /locus_tag="O0394_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499781.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            complement(2222..2830)
                     /locus_tag="O0394_001706"
     CDS             complement(2222..2830)
                     /locus_tag="O0394_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HCY0499782.1"
                     /translation="MQLFETMKIDNGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLNAESKFETIVSPLPEKSSFTAKFQVLPKVVNPTFIKNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            complement(2830..3981)
                     /locus_tag="O0394_001707"
     CDS             complement(2830..3981)
                     /locus_tag="O0394_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HCY0499783.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHAMCTHSLAKDDIYAAAYSFEKAESINSTYEHQT
                     SYVSKYHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKNNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            complement(3965..4558)
                     /locus_tag="O0394_001708"
     CDS             complement(3965..4558)
                     /locus_tag="O0394_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCY0499784.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIR
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
     gene            4909..5577
                     /gene="queC"
                     /locus_tag="O0394_001709"
     CDS             4909..5577
                     /gene="queC"
                     /locus_tag="O0394_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCY0499785.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            5579..5998
                     /gene="queD"
                     /locus_tag="O0394_001710"
     CDS             5579..5998
                     /gene="queD"
                     /locus_tag="O0394_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCY0499786.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            6002..6715
                     /gene="queE"
                     /locus_tag="O0394_001711"
     CDS             6002..6715
                     /gene="queE"
                     /locus_tag="O0394_001711"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCY0499787.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
     gene            6814..7398
                     /locus_tag="O0394_001712"
     CDS             6814..7398
                     /locus_tag="O0394_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499788.1"
                     /translation="MIVYILINIAIVVLITGFNLYRHQMQHLSLSAMLLSITINAFIN
                     TFIIDKYNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVT
                     DFSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNSLDETAENNNKIKKLMIKGLIIINI
                     SFIVMMVLTPYWYLYLIVYLIFLLFLLWQKVYKF"
     gene            7678..8118
                     /locus_tag="O0394_001713"
     CDS             7678..8118
                     /locus_tag="O0394_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HCY0499789.1"
                     /translation="MNTKYFLAAGAVITTLALGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            8460..8933
                     /locus_tag="O0394_001714"
     CDS             8460..8933
                     /locus_tag="O0394_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCY0499790.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            8908..9594
                     /locus_tag="O0394_001715"
     CDS             8908..9594
                     /locus_tag="O0394_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0499791.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            9594..10649
                     /gene="saeS"
                     /locus_tag="O0394_001716"
     CDS             9594..10649
                     /gene="saeS"
                     /locus_tag="O0394_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HCY0499792.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIMSYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCNEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENEDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(10721..11704)
                     /gene="csbB"
                     /locus_tag="O0394_001717"
     CDS             complement(10721..11704)
                     /gene="csbB"
                     /locus_tag="O0394_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HCY0499793.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSLENDEKDTHKVYSK"
BASE COUNT         4080 a         2001 c         1726 g         4045 t
ORIGIN      
        1 taattttagt caaaattcaa gtaaaaacac actttatctt ttcattttac tgtgaaacga
       61 attagaaaat gatacatttc gcagataaaa cacagtatta tttcattata cattaggagg
      121 aaattatttg catcggactc gagtatgagc tacgtaccaa atattatttc atcatttcta
      181 attgcattct atattctatt ccatggaata attggtttaa ccagttacta tacttaacaa
      241 ggttcttttc agccgttaaa atatctataa attgaaattt caaacgtgat ccttgcggtt
      301 tttgtgctaa tttcgttaaa tgataacttg cgattgtacc gatttgcggg tagctaccta
      361 gcgtataatg atcatttaat aggataatag gtgtgccatc acgcttaatt tgtatcgttc
      421 cttttttgac agtctgatac ggtggcatat cttcataaaa agctttgatt ttttcacctt
      481 caagcatcat ccccatgcga tttgctttgc tcgttacttt ataatcatgt ttcacaaatc
      541 tttgaatggc atcttcttta aaatcttccg tacctttatt tttaacgaca tgaaatacat
      601 cagacatata attaaatgac aaggcgtatc catcaatacc ccaatctgtt tgtttcgtgt
      661 gagcaaggtt ttcaaataac ttatgatgac gagctgtata atctctctta agctttattt
      721 catcgccatc ttgtaatgtt ctacctttaa aaccaccaat ttttacatta aagtcggttg
      781 agttagatcc taaccatgca tctaattcaa atccgcctcc cacagctaaa tacactcgcg
      841 atgtataact tgtttctcta aactttaaaa catcgccttt atctaacaaa tacaatttat
      901 atggagatat agtcatatgc tcagtgtaag ctttgacatt accccctgct aatgcaatca
      961 gcgtaggttc tgtaaaacga atcgttgcca ttttattagt catttccaaa gttgcttcat
     1021 tcttgtcatt tgcaactaat cgattagcaa tttcatgtgc taaagtatca agtgcaccac
     1081 atggaattac accatcatgt tcatatcccc ttctgccaaa gtcctgaaag ctactgaata
     1141 agccactttt ttcaattatg attgacatct ttaaacgctc ctacttctaa ttcattttca
     1201 ttgataggtc taaatttaat attatcgcct aactttaagc gtgcgaaatc tgattctttc
     1261 ggattaaaaa gtgataatgg tgtataacca ataactaacc aatcattaat tgtatccgta
     1321 gttacaatac cgcatttttt cccttcaagt actacagaac cagctggaat gaattttttc
     1381 tgtttactcg tgtgattaat atacaatttc ttatttaatc cagttaaata aggaaaccca
     1441 ggtgaatatc ccatcatcga aacaaaatat tgcgccttag aatgtaattc aataaaagtt
     1501 tctagcttga ttttgtaatg ttttaaaagt gattctaaat ccggaccata tttcgaacca
     1561 tacacaattg gtatttcaat caaatcttgc tggttaaaat catgttttat gtttaaatga
     1621 atcgattcta ttagtgcttt catgtataaa aaaggtgatt gtatattatg gtgtttaatc
     1681 atatcccttg cgtcatagac aatcatcatg tctgattctg atggcacaat ttctataata
     1741 aatggataat tttgttcaat taaatatgag cgaagtgtta ataaatcttc ggttaaactt
     1801 ttagatactt ctttttcaat tgcgactaca atggcttggt caccttgact atatattttc
     1861 atttgtacac ctcttcaata tttggttact ttactttcat ttttaattat agaatcgatt
     1921 gaatagttca ttttacctac tcactaaatt tactggtggt acattaaatc cattgcccgc
     1981 acaacaaaag gcataagttc gtgaattaat gcgtatacaa ggataaagct tataacagta
     2041 gtaattgttg ctatcaaacg aacaacatat attctatttt cagatagcaa tcgattcact
     2101 aatcgataat ttacgtatac aagaattaac aacagcacaa tataaacaat aatcatattt
     2161 ggccccattt aatttttaaa tttgttttca catcattttc tactttatta tcatactaat
     2221 tttaaaggca aaggtggaca tcggcaacct ctcgtaaact atttatcaaa aataaacgta
     2281 tctcattgtt atgatattta taaatcaatt cgtttttatt aaagtctttt tcaacaagtt
     2341 tgtcactatc tattaaataa tcacgcatgc atccttttaa gaaatcatct ttataacttg
     2401 gtgtgtacca ttttccatcc tcttcaatga caatgttgcc aatatcaaat tcaaggacct
     2461 tgccgtcctc tgaagttagc aatattaaat ctgtttcatg attgtgtgct aaatgctttc
     2521 gttctgtcgt tttatttttt ataaaagttg gattaactac tttgggcaac acttgaaatt
     2581 ttgctgtaaa actacttttc tcaggtaaag gtgacactat cgtttcaaat ttgctttcag
     2641 catttaatac gattttaagt ctatattgac cactgtgata ctttgttgtt acatcgttta
     2701 attcatttcg ccatgcatgt tcatcaaatt taaagtttaa tcgctcagaa gaacatttta
     2761 tgcgattagt atgataagta agtctaggga tatgtccatt atcaattttc attgtttcaa
     2821 ataattgcat tataacatct ccaaaatctt ggtttttgcg taaaattcat tcacttcatc
     2881 ttttggctta gaatcaattg taatacctgc tccgactcca taaatcgctt gtccatattt
     2941 atactcaata gtgcgaatcg gaatattaaa aatcatttta tcatcttcag ttggaagtaa
     3001 tagtccaatt gctccgcagt atataccacg aggtgaactt tctaattgtt taatatattt
     3061 cattgtattc agtttcggtg cacctgtaat cgaaccacaa ggaaataacg atgttaaaat
     3121 ttgagttaag tctgtattat tttttaaagt tccacttacc atcgaagtca tttgaaatac
     3181 agttttatat gcctcaataa aaaatagttt atacacctta attgagccac tctgtgatat
     3241 tctccctata tcgtttctta gtaaatcaac aatcatgaca ttttctgcac gatctttcga
     3301 agaagtttgc aatgttttat aatactgttg atcttcagct tccgttttac ctctaggcat
     3361 tgtccctttc atcggtttgc ttatgataac gttatcgaca ttgttaaatt gtcctttttg
     3421 aaaaaataat tctggtgaga tagacgctac ttggatttca tcagtttgta ataacgcagt
     3481 ataattacca ttactaaatt gagttaatcg ttcatataaa gtactaatag gataataaat
     3541 gttatccgtt aagcgcgccg tatagttcac ttgatacgtt tcgccttcaa caattgcttg
     3601 ttggacacgt ttaatattag tcatcataac ctcagaagat tcaacaaatg aaaaatgata
     3661 ctttgataca taagaagttt gatgttcata tgttgaattt atgctttccg ctttttcaaa
     3721 actataagct gctgcataaa tatcatcttt agctaatgaa tgtgtacaca tagcatgatt
     3781 aaaatacttt gccgcttcgt aacttaaata taacgagaca tatctacctt gtcgttgctg
     3841 cgcttgtgca aagtgtatca cttctcccac atcagccaac ttagtagcaa catacttctt
     3901 tataaatccc tttaattgaa tatggtattg cttatattca ttttcagtta aatagtagcg
     3961 ataattatat tctattctca cagtaatcac ctaccttcga taaaaataat tcaacttgtc
     4021 gataaccgta ttcactcaaa atagattcag gatgatattg cacaccaaaa accggaaatc
     4081 taatatgctc aaatgccata ataatcgctt catcgttttt tgctgtaatc tttaagcaat
     4141 ttggaaaagt cgctccgtca gcaattaatg aatgataacg cattacattg aaattttgag
     4201 gcagtccttg aaaaatacct tcattggtat ggcgtaactg tgtagtatgt ccgtgtacag
     4261 gatgatagcc gtgaatgata tttccaccaa aataagacac gatacattga aatcctaaac
     4321 atacacctag tataggtaca cgctgataaa attgttctaa cacttcattc aagataggat
     4381 aatcatccgg attacccggc ccaggcgaaa taacaattgc ttttggcttc atattaatga
     4441 cgtcttctat cagcagatta tcaataccaa caacttgaac tgttagtttc gtttgagtct
     4501 taatatagtc tattaaatta tatgtaaatg aatcattatt atctatgact agaatcattg
     4561 tatactccgt tctaaatgtg ttttattttt ataatatgta ttggatgtag ctaaaacttt
     4621 aaaagcattg tcattatcct gaccttgatt ttaactaata tatggtatat tctattcatc
     4681 gtacataaat gaatatcaga ggttcctagc tgaaaccctc tataaaaaac tagacattga
     4741 aatttcaaac attcgttggt aaacctttaa tatatattat aggagcaacc catttctttc
     4801 gtctatcttt tatggagata ggcttttttt attttataaa ttaaaaacgg gcttatcaat
     4861 gccattaacc tctttattaa tattattaat gataggagca attacaaaat ggaaagtgta
     4921 ttaaataatg aaaaagccat cgttgtcttt agtggtggtc aagacagtac aacatgtctc
     4981 ttttatgcaa aaaaacattt caaagaagtt gaactcgtaa cgtttaatta tggccaaaga
     5041 catgatactg aaattgaagt cgcaaaacaa attgcacaag atcaaggaat gaaacatcac
     5101 gttttagata tgtcattatt atcacaactt actccaaacg cattaacaca acatgatatg
     5161 gaaattacta ataatgaaga tggtatacct aatacatttg ttccagctag aaatttactt
     5221 ttcttgtcgt ttgcaggcgc tctagcttat caaattgggg ctaagcatat tattacaggc
     5281 gtatgtgaaa cagacttttc aggctaccca gactgtcgcg atagttttat aaaatcaatg
     5341 aacgtaacat taagcctagc tatggacaaa gattttgtca ttcatactcc tttaatgtgg
     5401 ttaaacaaag cagaaacgtg gaaattaagt gatgaactcg aagttttaga ttatattcgt
     5461 acaaaaacat taacatgcta taacggtatc attggggatg gctgtggtga atgtccagct
     5521 tgtcatttac gtcaacgtgg actaaatcaa tatcttgaaa gtaaaggagc gctttaaaat
     5581 gttacaacaa atctatccta gtacaacgca tccatatcaa ttcgaattaa ataaagattt
     5641 taatttttcg gctgcacatc acattccatg tgaagaagca ggtatttgtc aaaatgtcca
     5701 tggtcatact tactttgtta atttaacaat tgtcggtgat aaactagatg acactggctt
     5761 cttagtgaac tttagccatt tgaaaaagat gatacacggt aaatttgacc atcaactgtt
     5821 aaataactta cctgctttta aaaacaaaat cccttcaact gaaatcgtag cggaaacaat
     5881 ttatcaaatt gttaaagaaa atttggcatc gctcgaacac caaccaaaat gtattcaagt
     5941 atttgtaaga gaaacaccaa caagttatgt cgtatttaga ccaaaggaac aggtgtaact
     6001 aatggctaaa attcctgtac tcgaaatatt cggtccaacc attcagggtg aaggtcgagt
     6061 aatcggtaga aaaacgatgt ttgtgagaac agctggatgt gattatcgct gtagctggtg
     6121 tgattctgca tttacttggg atggtagtgc taaaggcgat ataaaactca tgaccgctga
     6181 agaaatttat gatgaattaa aacgaattgg tggcgattta tttaaccacg ttacaatttc
     6241 tggtggtaac ccagcattaa ttaaaggtat ccaagagtta gttgacttat ttcaagataa
     6301 aggtattttc agcgcactgg agacacaagg cagtaaattc caaccttgga tgacacaaat
     6361 tgatgattta acgattagtc caaaaccacc aagttcaact atgacacctg atttaaaaaa
     6421 gttagatgaa gtgattacac aatgcgtacc ttcatcatta aacttaaaag tagttgtatt
     6481 cgacgataaa gattatgatt ttgccaaaat gatacaccac cgttatccag atattccctt
     6541 ctatttacaa gttggtaacc catatttatc agacagcgta gataatcata ccgaaaagtt
     6601 gttagaacgc tacgagcagt tggtggattt agttatgcaa agtaatgata tgaatcacgt
     6661 ttatgtgtta ccacaacttc atacattact ttggagcaat aaaaaaggtg tataaaggat
     6721 aacttatttt actttttgca agtcatatct aatgaataac aatatattaa ttgatataat
     6781 agttgattga agtataacga acaggagtcc atcatgattg tatatatatt aattaatatt
     6841 gctatcgtcg tacttataac tggattcaat ttatataggc atcaaatgca acatttatca
     6901 ttaagtgcaa tgttgttatc tattacgata aacgccttta taaatacatt cataattgat
     6961 aaatataact ttattactct gtgtactata acgatgttta tcatttggac gatactacag
     7021 ttttacattg ataaaaaatt aaaacctgta tacattacag accaaaaatt tattgctatc
     7081 atattaacga ttgttgtcag tttaacgcaa cgtgttaccg acttttcaag tacacaatca
     7141 atatatatgt ctattccatt tttagcccct gccattttca tcattggtgg tattatgttg
     7201 tttatttcaa catttaatag cttagatgaa actgctgaaa ataataacaa aataaagaaa
     7261 ctaatgatta aaggacttat cattattaac atttcattta tcgttatgat ggttttaaca
     7321 ccatattggt acttgtattt aatcgtctat cttattttct tgttgttttt attgtggcaa
     7381 aaggtttata aattttaata ccaaaactat taaacacttc tgatattctt agttcaaaat
     7441 atcagaagtg ttttatagtg ttatctagtt cagataaata tttccttact taaaaaaacg
     7501 ccctcctctt attttgaccc ctatttattt aaatcagaca attattttca ttttcaaatt
     7561 attctttctt caatattagt taagcgatat ttaaacgaag ttaagaatta gttaatggca
     7621 tattatttgc cttcatttta aacttaactt atcaaattga agaaatgagg agttagcatg
     7681 aatacaaaat attttttagc agctggtgct gttatcacaa cattagcttt aggtgcttgt
     7741 ggtaattcta attcacaaga tcaaggtaac aaaactgaac aaaaaacaaa gtcagaagat
     7801 agcaatgtta aaactgataa aacaaaacac ctaacaggta cattcagttc taaaaacggt
     7861 gaaactgttg aaggtaaagc tgagattaaa aatggtaaat taatgcttac taactacaaa
     7921 tcatcaaaag gtccagattt atacgtctac ctaacaaaaa atggcgacat taaaaacggt
     7981 aaagaaatcg caatggttga ctacgataaa gaaaaacaaa catttgatct taaaaatgta
     8041 gatttaagca aatatgatga agtaacaatc tattgtaaaa aagctcacgt catcttcggc
     8101 ggcgctaaat taaaataatc aatttctgag ttaaactttt atttacaaca tactattact
     8161 atacattaca aatttttaaa atatacatta cactcattac tcaatggaaa gcgtatgatt
     8221 tcacagcccc cctagcttgt agaaatcata ctttcctttt ttcaatatat atacaactat
     8281 taaatcccat aagattgcag agcacataag taaatttttt tagagcttga ggtttgttta
     8341 gcttaagcaa cccatgagct caaacacttc ctgttcacat aacactacaa atcgcattat
     8401 gttgcttaat cttatgttta tataaattac acacaataaa tagaaagaat gtgaacatca
     8461 tgaataaatt attgctactc gttacattta tcattcgtgt gggttcaggt attgttatgt
     8521 taatgcaagg ctacgaaaaa ttaacgggcg gatttacgct gaaaggttta gtaccagtca
     8581 tcgctaacaa tactgattca ccagagtggt ataagtggtt tttcgcaaat atagttgcac
     8641 atacgacgtc attatttgat attgttgtcc cactcggaga gattgcaatt ggattaggtt
     8701 taatttttgg agtttttgca tatgctgcta gtttctttgg agcctttgtt atgataaatt
     8761 atatcttagc agatatgata tttacgtatc ctcttcaatt aactttcttt atccttttac
     8821 taatgagtca ctcattgtta aaacagattt cacttaaaga aatcattaat tactttagag
     8881 gtcgtaagaa cagaggtgaa aaaatagatg acccacttac tgatcgtgga tgatgaacaa
     8941 gacattgtag acatttgtca aacctatttt gaatatgaag gttacaaagt aacaacgaca
     9001 actagcggta aagaagcaat ttctttacta tcaaatgata ttgatatcat ggtacttgat
     9061 atcatgatgc cagaagttaa tggttacgac attgtcaaag aaatgaaaag gcaaaaatta
     9121 gatatcccct ttatctattt aactgccaaa acacaagaac atgataccat ttacgcctta
     9181 actttaggtg cagatgacta tgtcaaaaaa ccatttagtc caagggaact cgttttacgt
     9241 attaataatt tacttacaag aatgaagaaa taccatcatc aaccagttga acaactgtcg
     9301 tttgatgaat taacacttat taacttaagt aaagttgtga ctgtaaatgg tcacgaagtc
     9361 cctatgcgta ttaaggaatt tgagttattg tggtatttag cttctagaga aaatgaagtt
     9421 atttctaaat cagaattact tgaaaaagtt tggggatatg actattacga agatgctaat
     9481 accgtgaatg tccatataca ccgtattaga gaaaaattag aaaaagagag ctttacaaca
     9541 tataccatca caactgtatg gggattagga tataaatttg aaaggagccg ataatggtgt
     9601 tatcaattag aagtcaaatc attattggcg tcgtatcgag tatactatta acttcaacta
     9661 ttttagcaat tgcatatatt ttaatgtggt ttaacggcca tatgacacta actttgacct
     9721 taacgacaat aattacaagc tgtttaacct tattaatatg tagtattttt attaatccac
     9781 ttatacaaaa aattaagcag tttaatataa aaactaagca atttgctaac ggaaattacg
     9841 caagcaatga taaaacgttt aattcaccaa aagaaattta tgaattaaat caatctttta
     9901 ataaaatggc ttctgaaatt acgcaacaaa tgaatcaaat taaatccgaa caacaagaaa
     9961 aaacagaact gattcaaaac ttagcccatg atttaaaaac acctttagca agcattatgt
    10021 catattctga aggactacgt gatggtataa tcactaagga tcatgagatt aaagagtcat
    10081 acgacatatt aattaaacaa gcaaacagat tatcaacatt atttgatgat atgactcata
    10141 ttatcacttt aaatacaggt aaaacatatc ccccagaatt aatacaacta gaccaattac
    10201 ttgtatcaat attgcaacca tatgagcaac gtatcaaaca tgaaaaccgc acattagaag
    10261 tgaatttctg taacgaaatt gatgcatttt atcaatatcg aacgccactt gagcgtattt
    10321 taacaaactt acttgataat gcgctaaaat tttcaaatgt tggtagtcgc attgatatta
    10381 atattagtga aaacgaagat caagatacta tcgacattgc tattagcgat gaaggtattg
    10441 gcattatacc agaactacaa gaacgtatat tcgaacgtac attcagagta gaaaactctc
    10501 gtaatacaaa aacgggtggt tctggattag gcttatatat agctaatgaa ctcgcgcaac
    10561 aaaataacgc aaaaatcagt gtaagcagtg atatagatgt aggaactacg atgactgtaa
    10621 cattacacaa attagacatt acgtcataat ccgatttatt tataaaataa aatgcaaaga
    10681 ctaaaaagaa gctcccatta atgagggctt ctttttttgt ttatttagaa taaactttat
    10741 gggtatcctt ctcatcattt tcaagacttg aaagatttgt agcttgaata atatatttag
    10801 gacgtgcctt aacttcataa tatatcctgc caatatattc acctacaaca ccaattgaaa
    10861 ttaactgtat gccgcctaat aataaaatag ctgcaatcgt tgaaaaatat cccggaatat
    10921 taacaccaga tatcataata ttgatgaata aatagataat atataacacg cttattgaaa
    10981 agataaacaa gccaagataa atcatcattc tcaaaggttt actattaaag gaaatcaatc
    11041 catcaatacc ataattaaat aactttttaa aggaccactt agattcccct ttttgtctct
    11101 caacattttg atacgtaaac actttagtat tatagcctat ccattcaaat aacccttttg
    11161 aaaatcgatt atattcttca agtgatgcaa tggatttaac agctctttgg cttaaaagtc
    11221 taaaatcacc aacaccatca tcaaattgta cttcttctac aaagcaatta actaacttat
    11281 aatacaaatg gcttaatgtt ttgcgactaa aattttcacc acttctatca cgctttgcaa
    11341 tcacttgatc atagccttcc ataaaacctt caaccatttt cggaatatat tcaggtggat
    11401 gttgcaaatc acaatctatc atgatgactg catcaaattc agtactatgc tggtaacctg
    11461 caatcatagc tgcttctttt ccaaaatttc tactaaaaga aataaagctg acatgcctat
    11521 ctattgtggc aagatgttgc atttcatcta tagtggtatc cgtactacca tcatctatga
    11581 aaagcatatt ataatcatag cctttcacac tactatcttg tgaaagtatt tcagttaatt
    11641 gttgatgtgt ttgtgtaatg acttcccctt cattaaaaca aggaatgacg actctaattt
    11701 tcatctatgt ccatcctttt tatttaaaag attttgaatg tacctgagta ttgttttatc
    11761 aaatagtaaa aaccaactca tttattttaa tgtacacatt aaaaggcatt cataaacgta
    11821 aaatttaaca tccagttatt ttaaatcccc aa
//
LOCUS       DAKTLH010000077        11740 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.077, whole genome shotgun sequence.
ACCESSION   DAKTLH010000077 DAKTLH010000000
VERSION     DAKTLH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11740
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.077"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            105..3059
                     /gene="fdhF"
                     /locus_tag="O0394_001718"
     CDS             105..3059
                     /gene="fdhF"
                     /locus_tag="O0394_001718"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCY0499794.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTAIKEKHGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIDEWVDDFDEYYK
                     SLETFTMAFAEEATGIPESELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGEVHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALEPLGDSKPDWKIFQAIANRLG
                     FDWNYKHPSEIMDEVARLTPLYAGVSYDRLEGFNSLQWPVQPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEIYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRQPQYSVQVQKKWER
                     SDYVFPGNQVDK"
     gene            3059..3532
                     /locus_tag="O0394_001719"
     CDS             3059..3532
                     /locus_tag="O0394_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HCY0499795.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAIRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTTIRSLLGVLKDDDMNRSLTYMLNMLKGMSREE"
     gene            3760..3993
                     /locus_tag="O0394_001720"
     CDS             3760..3993
                     /locus_tag="O0394_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499796.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            4015..4791
                     /locus_tag="O0394_001721"
     CDS             4015..4791
                     /locus_tag="O0394_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCY0499797.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            5102..6226
                     /locus_tag="O0394_001722"
     CDS             5102..6226
                     /locus_tag="O0394_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HCY0499798.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLFVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(6318..7271)
                     /locus_tag="O0394_001723"
     CDS             complement(6318..7271)
                     /locus_tag="O0394_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HCY0499799.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(7633..8133)
                     /locus_tag="O0394_001724"
     CDS             complement(7633..8133)
                     /locus_tag="O0394_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0499800.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
     gene            complement(8370..8792)
                     /locus_tag="O0394_001725"
     CDS             complement(8370..8792)
                     /locus_tag="O0394_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HCY0499801.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITLIGVFLIATDIDFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKYYNGQEYIIPLTIRFI"
     gene            9040..10122
                     /locus_tag="O0394_001726"
     CDS             9040..10122
                     /locus_tag="O0394_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HCY0499802.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
     gene            10217..11617
                     /gene="nhaC"
                     /locus_tag="O0394_001727"
     CDS             10217..11617
                     /gene="nhaC"
                     /locus_tag="O0394_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCY0499803.1"
                     /translation="MMKRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNESMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVEEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
BASE COUNT         4073 a         1651 c         2242 g         3774 t
ORIGIN      
        1 attaattcta tctgaaagat gtgtggggca tcgttatttt aggtggatat gagcaattta
       61 ttaaaagtca tttacggaaa atatatatag acggggtgag taatatgcaa gaacatttgg
      121 tggttacact tgatggaaaa gattatcttg tagaaccagg tacgaattta cttgaattta
      181 ttaaatcaca agatactttt gtaccttcaa tttgttataa cgagtcgatg ggaccaattc
      241 aaacatgtga tacatgtact gttgagattg acggtaaaat tgaacgttca tgtagtacgg
      301 tgattgatcg tccaatgact gtaaatactg tgaacaatga tgtgaaagat gctcaaaaag
      361 aagcgcttga tcgaatttta gaaaagcata tgctgtattg tacagtatgt gattataata
      421 atggtgattg tgaaattcat aatacgatgg atgcatgggg gcttcagcat caaacgtatg
      481 aatataaaga gaagccatac gaaaaagatt acggcccatt ttatcgttat gatccaaatc
      541 aatgtatctt atgtgggcgt tgtgtagaag catgtcaaga tatcgaagtg aatgaaacta
      601 ttagaattga ttgggatcgt gaacatccac gtgttatttg ggataatgat gtaccgatta
      661 atgagtcttc atgtgtatct tgtggtcaat gtgcgacggt atgtccatgt aatgccatga
      721 tggaagtgaa tatggaaggt aatgcgggtt atatgactga tacggaacct ggttcattag
      781 cagcaatgat tgatttaact aaaaaagcag aacctggtta tggtccacta tttgcaatct
      841 cagattcaga agcagaaatg cgtaaagagc gtattaagaa aactaaaaca gtgtgtactt
      901 attgtggtgt gggttgttca tttgaagttt ggactaaaga tagagaaatt ttaaaagtac
      961 aaccatcaca tgattctcca gcaaataaaa ttgcgacttg tgttaaaggt aagttttcat
     1021 ggggacatat taattcagat caacgattaa ctaaaccact agtaaggaaa aatggtgagt
     1081 tccatgaagt agaatgggat gaagctttaa acgtcattgc agataatttt acagctatta
     1141 aagaaaagca tggcccagat gcactatcat tcatttcttc ttctaaagcg acgaatgaag
     1201 aatcgtattt aatgcaaaaa ttagcaagac aagttattgg cacaaataac gttgataact
     1261 gttcaagata ttgccaagca cctgcaacaa aaggcttatt tagaacggtt ggacacggcg
     1321 gtgactcagg tagtattgaa gatttagaaa aagcggcaat gtctgtattg ataggtacta
     1381 atacagccga agctcatcca gttatcgcat cacgcatgaa acgtgcacaa aaattatttg
     1441 gtcaaaaaat acatgtattt gatattagaa aacatgaaat ggcagaacgt gcggatcgtt
     1501 tttatcaacc taaaccaggt acggatttag cgtggttaag tgcagtgact aagtatatta
     1561 ttgatcatga tttacacgat aaagcattta ttgatgagtg ggtagatgat tttgatgaat
     1621 attacaaatc attagaaaca tttacaatgg cttttgctga agaagcaaca ggtattcctg
     1681 aatcagaatt gattaaattt gctgaagaat gtgctaaagc tgaatctgtt gtaatttgtt
     1741 gggcaatggg tattacacaa caagacattg gtagtgactc aagtacagcg atttcaaact
     1801 tattattagt aacaggtaat tatcgtcgtc ctggtactgg tgcgtatcca ttacgtggac
     1861 ataataatgt tcaaggatgt agtgatatgg gaagtatgcc tgataagatt actggttatc
     1921 aaagtattga agcggatgat attcgcgcta aatttgaaaa agaatatggc gttaaattga
     1981 atccaaaagc tggtaaagat aatcatgaaa tggtagaagg tatacatgac ggagaagtac
     2041 actcattgta cttatatggt gaagatacgg gtattgtgga ttcaaatatt aattttgtac
     2101 aagctgcgtt tgaaaaatta gatttcatgg tagtccaaga tgaattttta acattcacag
     2161 caacatacgc agatgttgta ttgccagcaa gtccttcact tgaaaaagac ggtactttta
     2221 caaataccga acgtcgtatt caacgtttat accaagcatt agaacctctt ggtgattcaa
     2281 aacctgactg gaaaattttc caagcaattg ctaatagatt aggatttgat tggaattaca
     2341 agcatcctag tgaaattatg gatgaagtag cacgcttaac acctctatat gctggggtaa
     2401 gttatgatcg tttagaagga ttcaatagtt tacaatggcc agtacaacct gatggtactg
     2461 atgagcctat tctatactta gaaggattca attttgataa tggtaaagca aaattattcc
     2521 cattatcatt tgataattac tttaagcaag atgaaattta tgatattcat gtaaacaatg
     2581 gtcgattgtt agagcacttc catgaaggta atatgactta tcaaacacca atgattaaat
     2641 acaaagtgcc acgtgcattt gttgaaattt ctccagagct tgctgaagat agaggcattc
     2701 atgaaggtgc agaagttaag ctgatttctg aaacaggaga agcggtatta caagttcacg
     2761 tgacagatcg tgttaaaggt aaagaaatct atattccatt aaataatgat gcgatggaaa
     2821 atggagatct aggtgcaatt aacttattaa ctaatagtga tgttgatcaa tacacggata
     2881 caccatctta taaacgtact agctgtcgtt tggaggtaat tacgaaacgt ggtaagtcac
     2941 cgttgaaccc taataatttc cgtgtcaata aaaaacgtca accgcagtac agtgttcaag
     3001 tacagaaaaa atgggaacgt tcagattatg ttttcccggg aaatcaggtg gataaataat
     3061 ggctgagaga atatcttcta aaattagacg cttagaaaaa tcagaagaac aaattaagtt
     3121 agaaagttta aatgaagtta cagaggcaat tgcagcgaat aaagatagta tattaaaagc
     3181 aatcaagctt attaaaacat tagatgatgc aaaactatta gatgctttaa atggtgcaat
     3241 tcgaggacgt caagtcatca taaataaatt tgcagttgaa cttaataaag atatttatac
     3301 agggctatta tctaatatgg cttcaatggt atttttatta ggtgaactca atgtatcaga
     3361 tttaagcgac ttcctaaata aagttaataa aggattacac gttgccaatc aagcgagtcc
     3421 aaatgctaag acaactataa gaagtctatt gggcgtattg aaagatgacg acatgaaccg
     3481 tagtttaaca tacatgctta atatgctaaa aggtatgtct cgagaagaat aacattacaa
     3541 tgaaaaagtg tttctacatg caagcagtgt aggagcactt tttttaattt ttgtaaaaat
     3601 taaaaagggt gtttttgatg gaataaatga ataatattga aaaacaaaaa aatcacgtgc
     3661 tattgtgaat caatcatcga caggcctatg aaacatcatg tattattatg attatgtaag
     3721 ttatttaaat aaaaagggtg agtgcatatt aattcgagaa tgttaaaaac gtcagctgta
     3781 atttctaata cattattagt cataggttta gtctgtctat ttatgatgaa attagtgtta
     3841 gctattactt tttttgctgt ttcattatca attagtctag ttgtctttaa tatgatgttc
     3901 agagatagaa caacgatgaa agtaatcgtt aacgcttctt ttttaatcgt aatattagcg
     3961 attgtagtgg catatttttt attgtctaag taagaaaaat gaggttttgt ctatatgaag
     4021 aagaatttca agttacgcat ttcaacgcta ctattgatag ttattttagt tgtttttgct
     4081 gtattactca tcgtgaatga aactaaattg tttaaaaatg atgtgaatta ctcttttgat
     4141 gaggctgttt caatgcaaca agggaaaggt attgtacaga caaaagaaga ggatggtaaa
     4201 tttgttgaag caaataataa tgaaattgct aaagcaatga ctatttcaca taaagacaat
     4261 gatatgaagt atatggatat cacagaaaaa gtgccaatgt cggaatctga agttaaccaa
     4321 ttgctaaaag gtaaggggat tttagaaaat cgagggaaag tttttctaga agctcaagaa
     4381 aaatatgagg ttaatgtcat ttatcttgtt agccatgcat tagtagaaac aggtaacggc
     4441 aaatcagaat tagcaaaagg cattaaagat gggaaaaaac gctattacaa cttttttggt
     4501 ataggagcat tcgatagtag tgctgttcgt agtgggaaaa gttatgctga aaaggaacaa
     4561 tggacatcac cagataaggc gattattggt ggtgcaaagt tcattcgtaa tgaatatttt
     4621 gaaaacaatc aactgaattt atatcaaatg cgatggaatc cagaaaatcc tgcgcaacat
     4681 caatatgcga gtgacattcg ctgggcagat aaaattgcca aattaatgga taaatcctat
     4741 aagcagtttg gtataaagaa agatgatatt agacaaacat attataaata agacatcggt
     4801 gcttaaagga gctggaacaa tttattgttt cgagctcctt tagcgcattc tgagtgtgtt
     4861 agttaaatgg attttaacct aacaaaaaac gctatatagc atcaaatatg ctatatccca
     4921 catcattgtt acaaatgtac atgatgtaaa tgaatattgc tgtctaaatg tgcatgtaat
     4981 atacaatggt gcagataata cacttaagtc cttaaaaatg aaacgttagt tccaagagtc
     5041 atttttaaac aatagtgcat gtgataaaat agaaaagaat gaaaaatata gaggtgacaa
     5101 tatgaagata gcaattatag gtgcaggcat cggtggatta acagctgctg cattattaca
     5161 agaacaaggt catactatta aagtctttga aaaaaatgag tcagttaaag aaattggcgc
     5221 tgggattggt atcggagata atgtgcttaa aaaactaggt aatcatgact tagctaaagg
     5281 tattaaaaat gctgggcaaa tcttatctac aatgacagtg ttagatgaca aagatcgcct
     5341 gttaactact gttaaattaa aaagtaatac attgaatgtg acgttaccac gccaaacatt
     5401 aattgacatt attaaatctt atgtaaaaga tgacgcaata tttacaaatc atgaagtcac
     5461 gcatatagat aatgagacag ataaagttac catacatttc gcggaacaag aaagtgaagc
     5521 atttgattta tgtattggtg ctgatggaat tcattctaaa gtgagacaat ctgtaaatgc
     5581 tgacagtaaa gtattatatc aagggtatac atgctttaga ggtttaattg atgatattga
     5641 tttaaagcat ccagattgtg caaaagaata ctggggaaga aaaggaagag taggtattgt
     5701 tccgttatta aataatcaag catattggtt cattacaatt aactcgaagg aaaacaatca
     5761 taaatatagt tcgtttggta aacctcattt gcaagcatac tttaatcact atccaaatga
     5821 agttagagag atcttagaca aacaaagtga aacaggtatc ttattgcata atatttatga
     5881 tttgaaacca ctcaaatctt ttgtttatgg tcgtactatt ttactaggag atgcagcaca
     5941 tgcgacaacg cctaatatgg ggcaaggtgc tggacaagca atggaagatg ctatcgtatt
     6001 agtaaattgt tttaacgcat acgactttga aaaagcatta cagcgttatg ataaaatacg
     6061 tgtcaaacat actgcaaaag taattaagcg ttctagaaaa atcggtaaaa ttgcccaata
     6121 tcgtagtcgt ttatttgttg cagttagaaa tcgtattatg aaaatgatgc caaatgcatt
     6181 agcagctgga caaactaaat tcttatataa atcgaaagaa aaataataca acaatatgaa
     6241 aacccccgta tgttgaaacg agagctcaac atatgggggt tcttgttttt ataatgttat
     6301 tataataaat tcaattatta gttaacgaca aattgtggtt tctcaccttg aacggcacta
     6361 attgcagcat tagcaacaat tttagacatc atgtcacgtg cttcaaatgt agcattacca
     6421 atatgcggtg ttaatactac attattaagt gattttaagt catcggtaat atctggttca
     6481 aattcatata catcaagtgc agcaccttca atttcattat ctttcaatgc ttgcactagt
     6541 gcttgttcgt gcacgattgg accacgagag gcattgatta aatacgccgt agatttcatc
     6601 attttaaatt gttctgtatc aattaaatga tgcattttag gattataagc agcgttgata
     6661 gtgataaaat ctgcattctt taatagtgta tctaaatcta catattttgc accgatttct
     6721 cgttcttttt cttctttgcg attaggtcca gtgtatagca catccatgtc aaatgctctt
     6781 gcacgacgag ctactgcact accaatttca cctaaaccga taatgccgat tgttttccca
     6841 gatacttctc tacctctgaa aaataaaggt gcccatccat caaatccagt tgtacgtgat
     6901 aattggtccc cttcaacaat acgacgcgct actgcaagta ctaatccaat tgttaaatca
     6961 gcagtcgcgt ttgttgatgc tttaggtgtg tttgtaacat ctatactttt ttctcgggca
     7021 tactcgatat caatattatt aaaaccagcg ccatagttgg caatgatttt taagtcttta
     7081 ccagcatcga taacatcttt atcaacgttt gtagataata aactaattaa ggcagtcgcg
     7141 tttttaacac ctttaattaa agtgtcttta tcgactaatc ctttaccttc atacatttca
     7201 acttcaaaat gttcttgtaa aagttttaaa cctacttctg gtattgcacc agcaacataa
     7261 actttttcca taaaagatca ctccttttat cttagtatag tagaagatta gacagtatac
     7321 aactatgtca tgatgtcttg tgtatcaatg atgtaagcgc gtacttttga tggaggtgat
     7381 ataacttagg cactgtagaa ctatgaatat tgtaatgtgg aaaaactgga tcaattaaat
     7441 tagataacgt agttttaaag ttaatagtat tagaaaaaat taatattttg aatatgggag
     7501 gaaatataaa taagtaggtg gcaacgaaaa atagcaaaaa aagagcttct cctataaagg
     7561 aaagctcaaa gttttttgat gacatatgta ctagaattaa gtttcaagac aatatgtatc
     7621 atcgtgttta tattaaatat ggatgtagtt gtagttacct gcttcacttg cagaaatagt
     7681 tctagaactt actgagaaag gtccgccact ataattcatt tctgaaattg taactgaacc
     7741 atcactgttt acactttcta catatgcaac gtgaccaaat ggtccttcag aagattgtag
     7801 gatagcgcct ttagaaggtg tatgatttac tgtgaatcca gcaccttgtg cagcagcagc
     7861 ccaattatta gcatttcccc aagtagaacc gatttctccg ccaactttat catatacata
     7921 ccaagtacat tgtcctgcag tgtataagtt accagaatgt gaaattgatg atgtagttgt
     7981 cgtagttgtc gtagtcgttg tagtttgagt cgtgttgtag ttatagttgt tgtaatttgt
     8041 ataattttca gcagcatctg catgatgtgc ttgacctact aatgctgtgc cgattcctgc
     8101 tgttaacgta gttgctgtta ctaatttttt catgaataaa gtcctccaaa gttctatatc
     8161 tttttttata aataaaacgt agcgactgtt ttattctcac atctcgaatt gatgacaata
     8221 gttactttaa caaaattaat gcttcttgtg gggaatgtta ttgatttgta aaagaataaa
     8281 aaaactttga ctaattttgt aataaaaatt agtcaaagtt acaatgagat taacagataa
     8341 ttaataggaa atatttattt gtaatatgtt taaataaatc gaattgttaa aggtattata
     8401 tattcttggc cattataata tttgacacac gcaataattg tgaatacaaa agataatatt
     8461 gagaaagcga atatggataa aataccgata aacgtaatga tgaaacctat aataataatg
     8521 aaatcaatat ctgtagcaat taggaaaacg cctattaaag tgataacgac taaaacgata
     8581 gaccaaataa tataagaaat cgtatagtta agataatttt ttccagcacg atcaactagt
     8641 ttcgattcat cttttttcaa taaccatatt atcagtggac caataataga tgtgaataaa
     8701 cttaataaat agataagcat cgccataatg ttctcatcat tggatttgcg attcggttga
     8761 tgatttgtta cgtcgttcat ttcagttgtc atattagaca ctcctttgaa aattgtaata
     8821 ttatctttaa ctataacaaa atataatcaa aaataaacat gtttattaaa caattattaa
     8881 aaataaaaat aattggtgga cgtcggcgtt taaataggtt aatttaaggt tatatatact
     8941 taacatttat aatgatgcgt aatgaattcg catcattttt atattgtctt acgtataatt
     9001 tgtttttaat tttaaccaaa gatagaaaga gggttgttta tgaaaatagc aattgtagga
     9061 tcaggaaatg gcgcagttac ggcagcagta gatatggtga gcaaaggcca cgatgttaaa
     9121 ttatattgtc gtaatcaatc tataagtaag tttcaaaacg caatcgaaaa gggcggattt
     9181 gattttaata atgaaggtga tgaacgtttc gtaaaattca ctgatattag tgatgatatg
     9241 gaatatgttt taaaagatgc tgaaattgtt caagtgatta ttccatcttc atacatagag
     9301 tattatgctg atgtaatggc agagcatgta actgataatc agttgatatt cttcaacatg
     9361 gctgcagcaa tggggtcaat tcgttttatg aatgttttag aagatagaca tattgaaaca
     9421 aaaccacaac tagcggaagc taatacgttg acgtatggta cgcgtgtcga ttttgaaaat
     9481 gcagcagttg atttatctct aaatgtacgt cgtatcttct tttcaacata tgatagaagc
     9541 tgtctaaatg attgttatga caaagtttca agtatttatg atcatttagt aaaagaggaa
     9601 agcttaatta aaacaaatct tgaaaatggt aatcctgaag tgcatccagg accaacatta
     9661 ttgaatgtcg gtcgcattga ttatgctggc gagttcgctt tatataaaga aggaattact
     9721 aaacatacag ttagattact tcatgcaatc gaattagaac gtttgaattt aggccgtaga
     9781 ttaggttttg aattatcaac agctaaagaa tcacgtattg aacgtggtta tttagaacgt
     9841 gataaagaag atgaaccatt aaatcgtttg tttaatacaa gcccagtatt ttcacaaatt
     9901 ccaggaccaa atcatgtaga aagcagatat ttaactgaag atattgcata tggtttagta
     9961 ctatggtcaa gcttaggtcg tgttattgat gtaccgacac caaatataga tgcagtaatt
    10021 gtaattgcat caaccatttt agagagagac ttctttgagg aaggcttaac agttgaagaa
    10081 attggtttag ataagcttga tttagaaaaa tatttaaaat aaatgatggc ttgaagatag
    10141 aaaaggatat agcattatgc aaaagcaata aattgaagaa aagaggtttc tcatcaataa
    10201 gcgaagggga cgatagatga tgaaaagaaa acccaccttt ttagaatcaa tttcgacaat
    10261 gattgtaatg gttattgttg ttgtaacagg ctttgtgttt tttgatattc caattcaagt
    10321 attattaatt attgcctcag catatgccac atggattgca aaacgtgtag gcttaacatg
    10381 gcaagattta gaaaaaggca ttgcagaacg tttaaatact gcaatgcctg caattttaat
    10441 tatactagcg gtaggaatta tagtaggcag ttggatgttt tctggcacag tgccagcctt
    10501 gatttattat ggcttagatt tattgaatcc aagctatttt ttaatatcag ccttttttat
    10561 aagtgctgtt acatctgtag caactggtac agcatggggc tctgcatcaa ctgcagggat
    10621 tgcacttatt tctattggta atcaattggg gattcctcca gggatggcag cgggtgctat
    10681 tatagcaggg gctgtgtttg gcgataaaat gtcaccatta tcagatacaa ctaatttagc
    10741 ggcgcttgtt actaaagtta atatatttaa acatatacat tcgatgatgt ggacgacgat
    10801 acctgcatca atcataggtt tattagtatg gtttattgct ggatttcaat ttaaagggca
    10861 ttcaaatgat aaacagattc aaactttgtt atcagagctt gcacagattt atcaaattaa
    10921 catatgggtc tgggttccct taattgtgat cattgtttgt ttgctattta aaatggctac
    10981 agtgccagct atgctaatat caagcttttc tgccattata gtggggactt ttaatcatca
    11041 tttcaaaatg acagatggtt tcaaagcaac atttagtggt tttaacgaat caatgataca
    11101 tcagtctcat atttcatcca gtgtgaaaag cttgttagaa cagggtggta tgatgagtat
    11161 gacccaaata ttagtaacga tattttgcgg atatgcattt gcaggtattg tagaaaaagc
    11221 aggatgttta gaagtcttat taactactat ttctaaaggc atccattctg taggaagttt
    11281 aatatgtatt actgttattt gttgtattgc gcttgtattc gctgcaggtg ttgcttcgat
    11341 tgtaattatt atggtcggtg tgttaatgaa agatttgttc gaaaaatacc aagtctctcg
    11401 ttccgtttta tcaagaacac ttgaagactc aagtacgatg gtcttacctt taataccatg
    11461 gggaacatca ggtatttact atacgaatca acttcatgtc tctgttgaag aatttttcat
    11521 atggacagta ccatgttatt tatgcgcaat tatagcaatt atctatggtt ttacagggat
    11581 aggtattaaa aagtcatcga attcacgttt aacttaatgt gagcgtggaa tatatataat
    11641 atgttgaaac actttaatca tttataattg tagcggttat aatttgaaaa ggttttaact
    11701 tagaataaat atcctctata catatactga atatgttttg
//
LOCUS       DAKTLH010000078        11605 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.078, whole genome shotgun sequence.
ACCESSION   DAKTLH010000078 DAKTLH010000000
VERSION     DAKTLH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11605
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.078"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(124..789)
                     /locus_tag="O0394_001728"
     CDS             complement(124..789)
                     /locus_tag="O0394_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0499804.1"
                     /translation="MALVVEDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(789..1844)
                     /locus_tag="O0394_001729"
     CDS             complement(789..1844)
                     /locus_tag="O0394_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0499805.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            1980..2654
                     /gene="hssR"
                     /locus_tag="O0394_001730"
     CDS             1980..2654
                     /gene="hssR"
                     /locus_tag="O0394_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HCY0499806.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            2647..4020
                     /gene="hssS"
                     /locus_tag="O0394_001731"
     CDS             2647..4020
                     /gene="hssS"
                     /locus_tag="O0394_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HCY0499807.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTIDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNQDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVNQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSL"
     gene            4183..4626
                     /locus_tag="O0394_001732"
     CDS             4183..4626
                     /locus_tag="O0394_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0499808.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDNVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            4623..5078
                     /locus_tag="O0394_001733"
     CDS             4623..5078
                     /locus_tag="O0394_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HCY0499809.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAM
                     LAGWFPLTVHYLIIFTIIFIVVYVLIWIIQFFKNKNYVDTINKQLKQLK"
     gene            complement(5144..6622)
                     /gene="mqo"
                     /locus_tag="O0394_001734"
     CDS             complement(5144..6622)
                     /gene="mqo"
                     /locus_tag="O0394_001734"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCY0499810.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSILFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFDNLEQRGVGIQYKQNVLDIKKQKSGV
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(7003..8601)
                     /locus_tag="O0394_001735"
     CDS             complement(7003..8601)
                     /locus_tag="O0394_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCY0499811.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTFTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTISEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            8918..10591
                     /locus_tag="O0394_001736"
     CDS             8918..10591
                     /locus_tag="O0394_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0499812.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKSNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            complement(10744..11373)
                     /locus_tag="O0394_001737"
     CDS             complement(10744..11373)
                     /locus_tag="O0394_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499813.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDGNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSSNNQANN
                     NQKSSYVAPYYGQNAAPVARQIYPFNGNKNQALQQLPNFQTALNAANNEANKFGSNNK
                     VYNDYSIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
BASE COUNT         3914 a         2048 c         1649 g         3994 t
ORIGIN      
        1 tttttacatt gcctaagaac ttaatgtccc agcctgtttt ttgtaaaccc aaatcgtatt
       61 ctgttatcta acttttatac tactatgtct taaatcatta acatttagcg tctttacaag
      121 ccactaatca gttattttgc catcttctaa ttcaatcact cgatctgcat attcaaataa
      181 tcttcgatcg tgtgtaatca taataccaat catttgttgt tctttaattt gttgacgtat
      241 catctcaaca acttttgttg ctctatcggc atctaaactt gctgtgggct catctgctaa
      301 aatgattttc ggattattca taaatgctct cataatcgca acacgttgct tttcaccgcc
      361 agataactga tgcggatata cattcaagcg atcttctaaa ccaatatttt tcaaaagttg
      421 tattgctctt gttgaacttt gttgtttggt cattcccgct tcttgaccta cgagtgtcaa
      481 ttgctctatc acttttaaat aaggaactaa atgtgaagat tgaaaaataa aaccaatatc
      541 ttccaatcgt aaatcactag gacgatgctg tttatcaaac aatggcgcat cattgtaaag
      601 cactgtacca ctcgtttgac ttaacaatcc gcctaatatc gttagcaatg ttgttttccc
      661 agaaccagag gcaccattta aaatgacaaa ttccccttgt tccacttcaa aattaatacc
      721 ttttaaaact tttgtttcag acaaaccttc tccgaaattt ttgacgatat cttcaacgac
      781 taatgccatt attctgcacc tccaattgct tcgataggat ccactttaaa taatttgata
      841 aatgatagtg aggcacctaa aatcgctact aatataaata tccccaccat taataaaata
      901 ttttgcgttg ttaagtaaaa aggcatcgtt accggcatca taaatgatag tcctactatg
      961 atgatcacag caatacctac cccaattatt gttagtgtta aaatttgtaa aactaacgca
     1021 ctcaataaat gtcttgtctt aataccaatt gctttcaaaa tgccaatttg tgatattttt
     1081 tgaatcgtca taacatagaa aaatgcactt agaacgattg ctgtaatagc aaacaaacta
     1141 acaatcatca tatttaacgg tgcttgctct gcttgataac tcgcaatatt acctgttaaa
     1201 tctttttcac tcacaacttg aacgtcattt attttattaa gctcatcacg ttgttgttgt
     1261 gttaaatttt tcactggata aaatgccgta ttaaccttat tcaatgcatt aaatccgtta
     1321 tcgttcaata gcacaatgga actatgcgca tacattgtgt cgtttaaaat accagaaact
     1381 ctataactag ttgaattatt tttaaaatgt aatctatccc cgactctaat gttgtctgca
     1441 gctaacttat cattaatagc gacctcatga gcatttttag gataatcacc ttttttaagt
     1501 tgtatgcgtt gttgtttaac gtcattcatt gtaatcacat cttgttcttt attagataat
     1561 ttcaatgttt gaatattcat tttatatggt tcttgatgaa ttaccttttt aatttgattc
     1621 tgttgcgtat cactaagttg cgatttctca atttgcggct ctttcatttt ttgaacaaca
     1681 tattcatcat tatcaaagtg ttcaaataac gaaatattct ccctaccaag cccttgagct
     1741 aaaccactaa taaataacac cataatactt aataaaagaa tgattaatgt gattaaaata
     1801 taacgaaatt tgtaaaacat aatctctttt atcgctaatt tcatatcgat tcacttctcc
     1861 ctatttcttc tttagcgtca gtataaggtt gaaatatgaa ctcaatatga acaattgttt
     1921 ttttacgcaa aaatatttta catgtataat aaatgaaaaa gataagggag tttatagcta
     1981 tggtgcaatg tcttgttgtc gatgacgatc ctcgaatcct aaattatata gctagccatt
     2041 tacaaacaga gcacattgat gcatacacac aaccaagtgg tgaagctgca ttaaaacttt
     2101 tagaaaaaca gcgtgtcgat attgcagtgg tagatattat gatggatggt atggacggct
     2161 ttcaattatg taatacatta aaaaatgatt atgatatacc agttattatg ttaacagcgc
     2221 gggatgcact tagtgacaaa gagcgtgcgt ttataagcgg tactgacgat tatgtaacca
     2281 aaccctttga ggttaaggaa cttattttta gaattcgtgc tgtattacgt cgatataata
     2341 tcaattcaaa ttcagaaatg actattggca acttaacgct aaaccaatcc tatttggaac
     2401 tccaagtatc taataaaacg atgacgttac cgaacaagga atttcaatta ttatttatgc
     2461 ttgcagcacg tcctaagcaa atctttactc gtgaacaaat aatagaaaaa atttggggct
     2521 atgattatga aggagatgag cgaacagttg acgttcatat taagcgacta cgccaaagat
     2581 taaaaaaatt aaatgccaca cttacaattg aaacagtaag aggacaaggc tataaggtgg
     2641 agaatcatgt ttaaaacact ctatgctaga attgcgattt attccattac ggttatttta
     2701 tttagtgcat taataagttt tgtattaaca aatgtttact atcattataa tttaaaagca
     2761 tctaatgacg cgaaaatcat gaagacgctt aaagaagcaa gacaatatga acaatcagct
     2821 aaaccaacgc acattcaaca atatttcaaa catttagggc aaatgaatta ccaaataatg
     2881 accattgatc aaaaaggtca taagacattt tatggggagc catttagaga agatacttta
     2941 tcacaaaatg ctattaacaa cgtattaaat aatcaagatt accatggtat taaagataaa
     3001 ccattcgcat tattcgtaac tggatttttc gacaatgtaa cagataatac tgttggtatt
     3061 aactttaaga caaaagacgg ttcaatagca gtatttatgc gtccagatat tggagaaaca
     3121 tttagcgagt ttagaacatt cttagccgtc ttgttaatgt tgttattatt tatctcgatt
     3181 tcattagtta tcgcatcaac ctattcaatc attcgtccag taaaaaagtt aaagctagcg
     3241 accgaacgct taattgatgg tgattttgaa acacctatca aacaaacacg caaagatgaa
     3301 attggaacat tacagtatca ctttaataag atgagagagt cattgggaca agttgatcaa
     3361 atgaggcagc attttgtaca aaatgtatct catgaaatca aaacaccatt aacacatatt
     3421 catcatttat taagcgagtt acaacaaact tctgataaaa cattaagaca acaatatata
     3481 aatgatatct ataccattac aacgcagctg agcggattaa caactgaatt gttgctctta
     3541 tccgaattag ataatcacca acatttatta ttcgatgata agattcaagt caatcaactc
     3601 attaaagata tcattcgaca tgaacaattt gctgctgatg aaaagtcgtt aatcatttta
     3661 gctgacctag aatcaatcaa tttcctagga aatcaacgtt tgttacatca agcattgagt
     3721 aatttattaa taaatgctat taagtataca gatgttggtg gtgccattga catcgctttg
     3781 caacatagtc acaacaatat cattttcaca ataagtaatg atggttcacc aattagtcca
     3841 caagctgaag cacgtttatt tgaacgtttt tataaagtga gcaaacatga taatagcaat
     3901 ggtttaggtc ttgcaattac aaaatccatc attgaattgc atcatggtac aatccaattc
     3961 acacaaagta acgagtatgt aaccaccttt actattacac tgccaaataa ttcactttaa
     4021 tctctacctc ctgaaaaaac atttctgttt ttatttcagg aggtatcttt tatattgatt
     4081 agcgctttct agccacttaa catttaattt ctaccattca acaaaattta aatatatcga
     4141 tggtcattta tggtttaata attttgaagg aggcattaac ctatgatgaa actcaattta
     4201 tttatcaatg caaatgagac ggaatcttat attgatatac atgcacctaa aatgaatgat
     4261 aacgttcaaa gtattattaa tgcagtcaat gatttagaca aatcacatac attagtcgga
     4321 tacatagaca aagaaatcca tatcattaat gtatcagatg ttataacatt ccaagttatt
     4381 aataaaaatg taacagccat tacaagcaat caaaaattca aactaaaatt acgactttat
     4441 gaactagaaa aacagttacc acaacatttc attcgcattt ccaaatcaga aatcgtcaac
     4501 aagtattaca ttgaaaaatt attattagag ccaaatggac ttatacgtat gtatcttaaa
     4561 gatgcacact acacctattc ttctagacgt tatttaaaat ctataaagga gcgcttatca
     4621 atatgaaaaa tttgaaaaac tcattattta ttagcttaat cattggtttg tccctttcac
     4681 tgttctttag tatgttattt gcagacggca aatattatcc actgaaccca caatcaacta
     4741 ttggcatatt gtattatacg catttcacag aaactaccgt aatgttgatt tctatcattt
     4801 tatggttact cataggcgtc gtctttttcc ttggagattt tatctttaaa tacacagatt
     4861 ggagcattac taaagcaact ataatgcatt tcataacaac gtatgtcgga ttcttgcctt
     4921 tagcaatgct tgccggttgg tttccactaa cagtgcatta tctaatcata ttcacaatta
     4981 tcttcattgt ggtttatgta ttaatttgga taattcaatt ctttaaaaac aagaactacg
     5041 tagacactat taacaagcaa ctgaaacaat taaaatgatt tttattaacc actaaaaaac
     5101 ggtaatgtct atcacattaa aaaagacatt accgtttcgt ttattattta acttgtaaat
     5161 acttagttac ttcttcatta attttatgat ataaatcctc atgatctgtt aagcgataac
     5221 caaatgacgg caccatttct ttaatctttg gttcccatcc tttgaattca tcacgatagc
     5281 atctctgtaa aacatcaaac ataatatcta cagctgttga cgcaccaggt gatgcaccaa
     5341 gtaatgcagc taatgtgcca tcatctgacg taataacttc agtaccaaat tgtaagttac
     5401 ctttagaatc ctcagtatcc ttgattactt ggacacgttg ccctgctgta atcacttccc
     5461 aatcttcatt tttagcattt gggaaaaaga ctcttaaatc atccatacgc tcatcattag
     5521 ataacatcaa ttgtgacact aaatacttcg taagactcat ttctttgata cctgcagata
     5581 acatcgtcac gatattattt ggtttaaccg atttaattaa atccatatgt gaacctgttt
     5641 ttaaaaattt aggtgagaaa cctgcaaatg gaccaaataa caatgaacgc ttgccgtcta
     5701 caaaacgtgt atctaagtgc ggcactgaca ttggtggcgc acccactgct gcttttccgt
     5761 acactttagc atgatgacga tcaatcactt cttgatttgt acagcgcagg aataatccac
     5821 ttaccgggaa accaccaata tgttttgatt gtttaatccc agtcttttgg agtaatggta
     5881 aactcgcacc gccagcacca ataaatacaa aatcagattc atatgttgtc gtttcattag
     5941 tttctaaatc tttaacttta actagccata ccccagattt ctgtttcttg atgtctaata
     6001 cattctgctt atattgaatt cccacaccac gttgctctaa attatcaaat aactttgcag
     6061 ttaacgcacc aaagttaaca tctgtaccag tttcatcata agttaaagca attggttcat
     6121 cagacttacg accttcaatc attaacggta cccaagattt catttcttct tcatcttgcg
     6181 ataatttcat tttttcgaat aaaatacttt tctttaacgt tgcaacacga ctttttataa
     6241 aagctacatt atcttcgcca atgacaaaac tcatgtgtgg caccgcttga ataaagcgat
     6301 ctgggttatc taattgtcct gttttaacta aatatgccca aaactgtttt gaaatttggt
     6361 actgctcatt tatcttaatt gctttactac aatcaactgt gccatcctta ccttcttttg
     6421 tatagttcaa ctcgcaaagt gctgaatgcc ctgtaccggc attattccat acatttgaac
     6481 tctcttcgcc aggttgtgct aatttttcaa acactttaat atttttctca ggtgataatt
     6541 ctttaagtaa tgttcctaat gttgcactca taataccgcc accaattaag atgacatctg
     6601 ttttgctatg ttgtgttgtc ataacaaata cagtccccct ttttaaaatg aataatggaa
     6661 cgttcgagaa atacgacata ttaataccac tttaaatcaa taagtgtctc aataggttcc
     6721 catggtgatg ctctattact atctactatg tatattaata ttttataata acgaaactac
     6781 tttaattata tactttatgt gcgatagcac aaagacaaaa ccacaaacct taacagctat
     6841 caaccaattt aatttgattg ctgattactc actattcatt ttaacataaa gcgctaccat
     6901 tattataatg tcaaacttct ttcattaatg atccataaaa aaaagatgag atgttagaaa
     6961 acatctcacc ttctgtatat ttatattttg aaatacgttt ttttataata atgacaagat
     7021 gaaagtccaa atacagatga atattagtaa acatacactg tatttcaatg tcatttttaa
     7081 taattctgat tccttaccaa cttgttttac tgctgcagtt gcaattgcaa ttgattgtgg
     7141 tgaaatcaat tttgccgcta caccaccaac tgtattagca gatacaagaa gtgaaccact
     7201 tgttccaatt tgttgtgcaa cagaagcttg aattggtgca aataatgagt tgttatttac
     7261 aactgatcct gtcataaaca cacctatcca acctaaaatt ggtgatagaa ctgggaagac
     7321 attacctgct ttagcaatac cttgacccat tgctgcactt aaaccaccat aagttgtgat
     7381 tttagaaatt gctaagatga aacaaattgt aagaactggt aaccacaact ctttaaatgt
     7441 aacgccgaat aatctacctg catctttaaa gttaaccttt ttagacatta aaattgtaat
     7501 aataatagtt aataaaatag ctgtacctgt ttgtccaata atatttaaag tcaatactaa
     7561 tggtttatgc gtaacttcgc tgattgttcc aggtaagttg aatttaaata ctaatgatga
     7621 taaagcacca tttggtaaga ataagttttt aaagaatggc gcactccaaa tcattacaat
     7681 gactgttaat acaatgaatg ggctccatgc atgtaatact gcttttgcag aatgtgcttt
     7741 tgcaggttca atttcttcat ctttattaac acgataaatg tgttttggtt ggaatttttt
     7801 agaaaatact gctaatgcta acattgttaa taacggtgga ataatatctg ctaattcagg
     7861 tccactgaat acagttaata atccttgagt aaatgtataa gtgattgaaa ctactaaaat
     7921 tgctggtaat gtttctttaa cacctctaaa accatcaata ataaagataa gtaagaatgg
     7981 aataatgaaa tttatgattg ccaatgttaa agttgctgat tgagaaacac ctaatactga
     8041 aacatctcca ggtaatttca acgtttctac aacacctaca gggataccaa tcgcaccaaa
     8101 agcaccagac gctgcatttg cgactaaaca taacatcgca gcttttaatg gattaaatcc
     8161 taattgtgtt aataaaagtg cacaaattgc aattggtaca ccaaatcctg ctgcaccttc
     8221 taaaaatgcg ttgaatgcaa atccaataag taaaacttga atacgttggt cttgtgaaat
     8281 atttgtaata ctatcttgaa ttgttaaaaa ttgtccagat tcaacagtaa ttttgtataa
     8341 caatactgcc atcataacga tatagccaat cggaatgata ccttggaaga aaccttcgac
     8401 tactgcacca gaagcgatac caactggcaa tttgaaaaat ggtattgcaa ttagtaatgt
     8461 tacaacaagt gttgtaatag ctgcataaat acctttcatt ttaaaaacag ttaaacatag
     8521 taaaaacagt acaatcggta ttgctgcaat taaagacgat aataataaat tatcaaatgg
     8581 gttaaacgta tttactaaca taggctgact cttctttcca taaagtagta gtgtaattgc
     8641 ttcattaaaa tcatgctctg gctatagcat tttgttatcg ataacatttt acagaacagt
     8701 gagattaatg tgattttttt cacaattaaa atataccatt ttttcagact attaacaata
     8761 tcactttcct atttaattgt tttatttttc attttaaatc tgttcatcaa aatgaattgt
     8821 acttaatgta atgaagtaac ttatactatt catgaatttt acataagacc gtgctaaaat
     8881 aaggaagata ctacgatgag gaatggaagt tatagcaatg attaaacaga taaatgtttc
     8941 taatatgcaa aagtttgaat ctcaattaat gaaagctcag tctgaaggtt atacacatgt
     9001 cgttccatat gccaatgaga taatgattta tcaatcgatg ttagatgcgg ttcaattata
     9061 tcctaagtct atcgtggttg attatactgt ggatggacaa tacaaaaatg attgtcacta
     9121 tttcggtcaa tcatcgatca acattgccga ttgggctcaa aacaataatt attatcctaa
     9181 cctgatatat gcgattcaac agactcttga tttaatacat tattactctg tagaaacgat
     9241 tttcgattta gctttattga cacttctgaa aggtgattta tcaatcgatg gtcatgtcgt
     9301 ttttgatttt aaagccccta tagcaacaag tgcttcaata tgggaaacta ttaaaactat
     9361 tgaagatttt gatatgatgt ctcagtttta tttgaataaa atggcttata ttgatcatca
     9421 tcctatacct tttcgcaacc tttttatcga ggattcagaa caacttaatt cgccagataa
     9481 ttggttgtat tctaccaagt ttatgctacc taaatggtta tataaaatag caaagcaacg
     9541 tgctgataac aaacaattac aaaacttcgg cctgtatacg aagcaaccaa acgttttaaa
     9601 agatcacatc gtttttatcg gtgatcatca tcaatatata ggtaattcaa aatatttatt
     9661 tacttacttc gttaaacata atccaatgac tgcctgttat tttgttactg acgatcgtcg
     9721 cggaccacat ttcatatcac ctaagtctga aaaagctgac gaattgatta acagcgcacg
     9781 cgtagttata gttgaaaatg atatacctga aacgttacaa ccaaacggta ctttaattca
     9841 attacatcaa ggcacaccaa taatgcaatt atttttagat agtaaagagc ctattaaaaa
     9901 tatagaaaca cctttttatc gtgctaaaag gtataatcgc tggttgcaat tcgattatgt
     9961 tatacattct gctgatgata ttagccactt ttatcaaacg gcttttccta gtcaccaagc
    10021 aaatgtattg gcttatggta atcctaaaca ccaatattta ctacaaaaga gtaacgaaag
    10081 tactactcaa caacagtata agaaatcgtt taaaataaat gatcaaaaac cagttttatt
    10141 atacgcacct attggattag tgagtgcgca acaattacca ctatcagatg ctttattcaa
    10201 agcctatcat gttgtggttc agggtgttga tgaagcgatc ttaccagaag aggcgcttgt
    10261 tgcaccaaag tatcttagtg ctcaagactt aattctaatg tctgatgtcg ttatcactga
    10321 ttactcaaat ataatatttg atgcgatggc tatagacaaa acagtagcat tatacactcc
    10381 aaatcacagt caatacattg agtctcaagg tgttaacgaa gatatttggc gtcatctatc
    10441 aaaaatttgg tatacagacc gacaattatt aattaataat ttaatttccc aggcaatacc
    10501 agtgattaaa tatccacaaa tacaacataa agaacaacca ttagaatcta tttcacaact
    10561 tatattgtct aagatgacat ctaatcaata gtgtttaaat ttctcagtgg ctgtgaatga
    10621 ggtttaaaag tactataaaa cgtaaacttt gatactttaa aatacgcaaa aaacggtaaa
    10681 ccctaattca tattatagag tttaccgttt tattttttaa cttgcatcat agttatatta
    10741 acattattgt tggtagtttg gatcagtaac cattgcttgt ccagtataat caaccgttac
    10801 aattgaatat tttccatttg catttgggtc tttaaaacta aacacatact tatagttgcc
    10861 attatgttct tcaatagaat aatcattata cactttatta ttactaccaa atttatttgc
    10921 ttcattatta gccgcattta aagctgtttg gaaatttggc aattgctgta aagcttgatt
    10981 tttatttcca ttaaacggat aaatttgacg tgcaaccggc gcggcatttt gtccataata
    11041 tggtgcaacg taacttgatt tttgattatt attcgcttgg ttattacttg attggttatt
    11101 atttgtttgg ttttggtcat tgtttgttgc atttgaatta gattgttgct ggttatcgtt
    11161 tgcactatta tctttattat ctttgtttac gtctttacta tcatctttat tatctttctt
    11221 atctttagat gaatcatttg tttttttatc ttgttgttca gttttcgctt tatcatcttt
    11281 ttctttatta ccgtcttttt gttggtcact atcttgacca catgcagcta aaaataatga
    11341 taatgctagt aaccctgtaa ctaatctttt catacatatc tcctcctata attcgatatt
    11401 cattgaataa tcttgaaata catatctacc atgtgtatct tttcatggct tgttaccaaa
    11461 gacgtcaaca ttaatatgat ctgaattatc acaatatcca taactacaat ctaaggttct
    11521 ttttattata ccctaatttt tgttcattat tatttaattt ttgtgaattt tatgttttct
    11581 ataaatttaa ttattttact ttaac
//
LOCUS       DAKTLH010000079        11302 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.079, whole genome shotgun sequence.
ACCESSION   DAKTLH010000079 DAKTLH010000000
VERSION     DAKTLH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11302
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.079"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            59..1012
                     /locus_tag="O0394_001738"
     CDS             59..1012
                     /locus_tag="O0394_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499814.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKYEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLN
                     LVHMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(1175..1378)
                     /locus_tag="O0394_001739"
     CDS             complement(1175..1378)
                     /locus_tag="O0394_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCY0499815.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            1677..2165
                     /locus_tag="O0394_001740"
     CDS             1677..2165
                     /locus_tag="O0394_001740"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCY0499816.1"
                     /translation="MAIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            2158..3093
                     /locus_tag="O0394_001741"
     CDS             2158..3093
                     /locus_tag="O0394_001741"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCY0499817.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQMLQLDVDLIVTAAFGQLLPESLLALPNLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            3090..4397
                     /gene="rsmB"
                     /locus_tag="O0394_001742"
     CDS             3090..4397
                     /gene="rsmB"
                     /locus_tag="O0394_001742"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCY0499818.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            4400..5494
                     /gene="rlmN"
                     /locus_tag="O0394_001743"
     CDS             4400..5494
                     /gene="rlmN"
                     /locus_tag="O0394_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCY0499819.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            5501..6244
                     /locus_tag="O0394_001744"
     CDS             5501..6244
                     /locus_tag="O0394_001744"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HCY0499820.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEQHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            6241..8235
                     /gene="pknB"
                     /locus_tag="O0394_001745"
     CDS             6241..8235
                     /gene="pknB"
                     /locus_tag="O0394_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HCY0499821.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV"
     gene            8463..9338
                     /gene="rsgA"
                     /locus_tag="O0394_001746"
     CDS             8463..9338
                     /gene="rsgA"
                     /locus_tag="O0394_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCY0499822.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFEVQNINEGYIHQVFERENELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARILVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            9339..9983
                     /locus_tag="O0394_001747"
     CDS             9339..9983
                     /locus_tag="O0394_001747"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCY0499823.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            9990..10631
                     /locus_tag="O0394_001748"
     CDS             9990..10631
                     /locus_tag="O0394_001748"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCY0499824.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(11012..11200)
                     /locus_tag="O0394_001749"
     CDS             complement(11012..11200)
                     /locus_tag="O0394_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCY0499825.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
BASE COUNT         4206 a         1558 c         2106 g         3432 t
ORIGIN      
        1 ttttaaattt atgtatgttt atttgattca aacaaaataa cttaagagga gaaagtttat
       61 gaaaaagaca ctgggatgtt tacttttaat tatgctttta gtcgtagcag gttgttcttt
      121 tggtgggaat cataaattat catcaaagaa atcagaagaa tcaaaacaag aaactgtaaa
      181 aaaagaatcg gaagaagaga aagatccaga tttagagaaa tatgaagaaa tagagaagaa
      241 aatgaaagga attaaagatg cgccatctct tgataagttg gatccattaa tgacagaaaa
      301 gtcgtttacg aatagtaaag ggattcaagg atggaaagat tacaaagaat taatgggtaa
      361 agtggaactt gcagattata gatttactaa agattcaaaa ggatcttcaa taaaagatgt
      421 tgatgcattc tttaaaggta agaaaggtat aaaaaggaaa gtgattgaaa cacacgatga
      481 tgtaaaacaa gttgattatt ggtatgtaga tccagatgga aagaaaattg gcaattcaaa
      541 cacacctgtt ttttacgcag aaattatgac aaaatataaa gatggaaagt tagtttatgc
      601 atcagtcgaa ccaggatctt acgtaataca taaagatgat gcaattaaat atgacgatta
      661 ttctaagtta aaaaaattaa gtcagctaac taaacttgat catccaaaac cagttccata
      721 tagcgtagct caaatcaaat ctttcggagt acctttaaca agcgtttcat ttatgacaca
      781 tggatcaaag gatactaaag atgaagtgtt gccggcattg gcctatttca ctttttcacc
      841 aaaaaattat gaagacaagt ctaatccaga tccaaaagtt ttaaatttag tacatatgga
      901 tttcttaaat gcatctagtg attttggtaa cgcacatttt gttgttttaa gtaaatatat
      961 taaagagtat gaatcaaact atgaaacagc gtcagatgat tctttaaaat agtatttact
     1021 gtgtgaaaaa taaatagtgt actacattaa ataatcgcaa taataatccc gataaacaat
     1081 cagcattact gcttatcaca tagagttcgt aataactata actctatgat tcgcaaataa
     1141 taaatgattg tcatcgggat ttatttttat caatttataa agtgacatta ccttgttcat
     1201 cagcaggttt gaaaacagta atcactgcac taataattgc taaaatgtgt gggatacctg
     1261 tccaacagaa tattaagtgt agaatacctt gcatattctt gccggcataa aatttatgaa
     1321 taccaaaact acctaagaac aatgctaata aaatataaat aactttgttt acttgcattt
     1381 ctttccctcc agttgaattg cttataatga cattagcttc tctttttatt atacccactt
     1441 ttagttcaaa cattctagtt taagcattcc caatcatcta aatttcagtt attcaatcct
     1501 tacaataaat ttaggattac atttcagttg cattgtatta ttttacgtgt gaaatatacg
     1561 taatgaatca catgacaatc ttcaaattga aaaatataca ttctatgatg taaggtcgca
     1621 tttttaatat atttacgtta taatagtttg atgaatttta ttgaggagta agagttatgg
     1681 cgattaaaaa gttagtacca gcatcgcatc ctattttaac gaaaaaagcg caagcagtta
     1741 aaacatttga tgattcgtta aaaagattat tacaagattt agaagataca atgtatgcac
     1801 aagaagctgc tggcttatgt gcacctcaaa ttaatcagtc attgcaagtg gcaattattg
     1861 atatggaaat ggaaggatta ttacaacttg ttaatccgaa aattattagt caatcaaatg
     1921 aaacaataac agacttagaa ggttcaatta cattgccaga tgtttacggc gaagtgacaa
     1981 gaagtaaaat gatagttgtc gaaagttatg acgtcaatgg gaacaaagtt gaactaactg
     2041 cacatgaaga tgtagcaaga atgattttgc atattataga tcaaatgaac ggtatccctt
     2101 ttacagaacg tgcggaccgt attttaacag ataaagaagt ggaggcatat tttataaatg
     2161 actaaaataa tatttatggg tacaccagac ttttcaacaa ctgttttaga aatgcttatt
     2221 gcagaacatg atgtcattgc agtcgtaacg caaccagatc gacctgttgg acgtaaacgt
     2281 gttatgacac caccaccagt taaaaaagtt gcaatgaaat atgatttacc tgtatatcaa
     2341 cctgaaaaat taagtggatc agaagaatta gaacaaatgc ttcaattaga tgtagattta
     2401 attgtaactg ctgcttttgg acaattatta cctgaatcat tgttggcatt accaaatctt
     2461 ggggcaatta atgtacatgc atcattgtta ccgaagtata gaggtggtgc accaattcat
     2521 caggcaatta tcgatggtga acaagaaacc ggcataacaa ttatgtatat ggttaaaaaa
     2581 ttagatgcgg gtaatattat ttcgcaacaa gcaattaaaa tagaagaaaa tgataatgtc
     2641 ggtacgatgc atgataaatt aagtgtatta ggggcagatt tattaaaaga aactttacca
     2701 tctattatag agggcacaaa tgaaagcgta cctcaagatg atacgcaagc aacatttgct
     2761 tccaatattc gacgcgaaga tgagcgaatt agctggaata aaccaggaag acaagtgttt
     2821 aatcaaattc gtggattatc accatggcca gttgcttata caactatgga tgacactaac
     2881 ttgaaaatat acgatgctga actcgttgag actaataaga taaacgagcc tggaaccatt
     2941 atagaaacga ctaaaaaagc cattattgtt gctacaaatg ataatgaagc tgttgcaatt
     3001 aaagatatgc aattagctgg gaaaaagaga atgttagctg ccaattattt aagtggtgcg
     3061 caaaacacac tagtagggaa gaaacttata tgatagaaaa cgtgagaagt cttgcttttg
     3121 acacgattca agatatatta aatgaaggtg cgtatagtaa cttgcgtatc aatgaagtgt
     3181 tgtcagaaaa tgaattaaat gcaatggata aggctttatt tacagaaatt gtctacggaa
     3241 ccgttaaaag aaaatatacg ttagattttt atttaaagcc ttttgtgaaa acaaagatta
     3301 aggcatgggt taggcaatta ttatggatga gtatttatca atatgtttat ttagataaag
     3361 ttccaaatca tgccattatt aatgaagcag ttgaaatagc aaaagaacgc ggtggctatc
     3421 ataatggtaa tgtcgtaaat ggtattttac gtacaatgat gcgtagtgac ttacctgatt
     3481 ttaatgaaat tgcagatcct aaaaaaagaa tggcaatcga gtatagtatg ccgaagtgga
     3541 ttatagatca ttgggcaaca cattatggtc tcgaagaaac tgaaacaatt ttacagtcat
     3601 ttttagaaac gacatcaaca actgtgcgtg ccaacctgac gcgagcatca ttagatgata
     3661 ttattgaaaa gttgcaagac gaaggttatg acgttgaaaa agatcatgac ttaccttatt
     3721 gtctccatat aggaggacaa ccaattattc attctcgttc atttaaagat ggattcgttt
     3781 caattcaaga taaaagctca atgtttgttg cacacattat gaatgtagac cgacatgatc
     3841 acgtattaga tgcatgtagt gcacctggcg gtaaagcttg tcacattgct gaagttttaa
     3901 tgccagaagg gcaagttgac gcttcagata tacatgatca caaaatagac ttaattaatt
     3961 ttaatataaa aaaattacga ttaacaaata ttaaagcttt tcaacatgat gcgacaaaac
     4021 cttatgataa aacatacgat aagatacttg ttgatgcacc atgtagcgga ttaggtgtaa
     4081 tgagacataa gccggagatt aagtatactc aaagcaaaca acatattgag tcactagttg
     4141 aattacagct tgaaatattg gaaaatgtaa aaaacaatgt aaaaataggt ggcgaaatca
     4201 tctattcaac atgtacaatt gagcaactag aaaatgaaaa cgtgatttat acgtttttga
     4261 aaaataataa aaacttcgaa tttgaaccgt ttcaacatcc gataactgga gagttggtca
     4321 aaacgttaca aatcatgccg caagacttta attcagatgg attctttatc actaagataa
     4381 aaagaaagga caattaggaa tgataactgc tgaaaagaaa aagaagaata aatttcttcc
     4441 aaattttgac aagcaatcaa tatattcatt gcgatttgac gaaatgcaaa actggctcgt
     4501 tgaacaaggg caacaaaaat ttcgagcgaa acagattttt gaatggttat atcaaaaaag
     4561 agtagattcg attgatgaaa tgacgaactt atcgaaagac ttacgacagc ttttaaaaga
     4621 taactttact gttacaactt taacaactgt agtaaaacaa gaaagtaaag acggtacaat
     4681 taaattctta tttgaattac aagatggcta tacaattgaa actgttttaa tgagacatga
     4741 ttatggaaat tcagtatgtg taacgacaca agtaggttgt cgcatcggat gtacgttttg
     4801 tgcttctaca cttggcggct taaaaagaaa ccttgaagct ggcgaaattg tttcacaagt
     4861 tttaacagtt caaaaagccc ttgatgctac agaagagcgc gtatctcaaa ttgtcataat
     4921 gggtatcggt gaaccatttg aaaattatga tgaaatgatg gactttttaa gaatcgtcaa
     4981 tgatgataac agtttaaata ttggtgcacg tcacattaca gtatcaacat caggtatcat
     5041 tcctagaata tatgactttg cggatgaaga tattcaaatt aattttgctg taagcttaca
     5101 cgccgcaaaa gatgaagtgc gatcacgctt gatgccaatt aaccgtgcat ataatgttga
     5161 gaagttaatc gaagcaattc aatattatca agaaaaaaca aatcgtcgtg ttacttttga
     5221 atatggtctg tttggtggtg tgaatgacca actagaacat gcaagagaat tagcacattt
     5281 aataaaaggc ttaaactgcc atgttaactt aattcctgtc aaccatgttc cagaaagaaa
     5341 ttatgtgaaa acggctaaaa atgatatctt taaatttgaa aaagaattaa agagactagg
     5401 aattaatgcc acaatacgtc gtgaacaagg ttcggatatt gacgcagctt gtggtcaatt
     5461 aagagcaaag gaacgacaag tagaaacgag gtaaagacaa atgctagagg cacaattttt
     5521 tactgatact ggacaacata gagataagaa tgaagatgcg ggtggtattt tttataatca
     5581 aactaatcaa caacttttag ttctgtgtga tggtatgggt ggccataaag caggagaagt
     5641 tgcaagtaaa tttgttacag atgagttgaa atcccgtttt gaagcggaaa atcttataga
     5701 acaacatcaa gctgaaaatt ggttgcgtaa taatataaaa gatataaatt ttcagttata
     5761 tcactatgca caagaaaatg cagaatataa aggtatgggt acaacatgtg tttgtgcact
     5821 tgtttttgaa aaatcagttg tgatagcaaa tgtcggtgat tctagagcct atgttattaa
     5881 tagtcgacaa attgaacaaa ttactagtga tcactcattt gttaatcatc ttgttttaac
     5941 gggtcaaatt acgccggaag aagcatttac acatccacaa cgtaatatta ttacgaaggt
     6001 gatgggcaca gataaacgtg tgagtccaga tttgtttatt aagcgattaa atttttatga
     6061 ttatttatta ttaaattcag atggattaac tgattatgtt aaagacaatg aaattaagcg
     6121 tttgttagta aaagaaggta caatagaaga tcatggtgat caattaatgc aattggcatt
     6181 agataaccat tcgaaagata acgttacttt catactcgcg gctattgaag gtgataaagt
     6241 atgataggta aaataataaa tgaacgatat aaaattgtag ataagcttgg cggcggtggc
     6301 atgagtaccg tttatcttgc tgaagatacg atacttaaca ttaaagttgc aattaaggcg
     6361 atttttatac cacctagaga aaaagaagaa acattaaaac gttttgaacg agaagtacat
     6421 aactcatcac agctatcaca tcaaaatata gtaagtatga tcgatgttga tgaagaagat
     6481 gactgttact acttagtaat ggaatatatc gaaggtccga ctttgtctga gtatattgaa
     6541 agtcatgggc cattaagtgt tgacacagcg attaatttta cgaatcaaat attggatggc
     6601 attaaacatg cgcatgatat gcgtattgta catagagata ttaagccaca aaatatatta
     6661 attgacagca ataaaacgtt gaaaatattt gattttggaa ttgctaaagc tttaagtgag
     6721 acgtctttaa ctcagactaa tcatgtgtta ggtactgtgc agtacttttc gccagaacaa
     6781 gcaaaaggtg aggcaacgga tgaatgtaca gatatttatt ctataggtat tgtgttatat
     6841 gaaatgcttg ttggtgaacc accctttaat ggagaaactg cagttagcat tgcgattaaa
     6901 catattcagg attctgtgcc aaatgtgaca acagatgtac gtaaggatat tccgcaatct
     6961 ttaagtaatg tcattttacg cgctacagaa aaagacaaag cgaatcgtta caaaacaatt
     7021 caagaaatga aagatgattt gagtagtgtt ttacatgaaa atcgagcgaa tgaagatgtc
     7081 tatgaactcg ataaaatgaa aacgatagcg gtacctttga aaaaagaaga tctagcaaag
     7141 catattagtg aacataagtc gaatcaacct aaacgtgaaa cgacgcaagt acctattgta
     7201 aatgggcctg ctcatcatca gcaattccaa aagccagaag gtacggtgta cgaaccaaaa
     7261 cctaaaaaga aatcaacacg aaagattgtg ctcttatcac taatcttttc gttgttaatg
     7321 attgcacttg tttcttttgt ggcaatggca atgtttggta ataaatacga agagacacct
     7381 gatgtaatcg ggaaatctgt aaaagaagca gagcaaatat tcaataaaaa caacctgaaa
     7441 ttgggtaaaa tttctagaag ttatagtgat aaatatcctg aaaatgaaat tattaagaca
     7501 actcctaata ctggtgaacg tgttgaacgt ggtgacagtg ttgatgttgt tatatcaaag
     7561 ggccctgaaa aggttaaaat gccaaatgtc attggtttac ctaaggagga agccttgcag
     7621 aaattaaaat cgttaggtct taaagatgtt acgattgaaa aagtatataa taatcaagcg
     7681 ccaaaaggat acattgcaaa tcaaagtgta accgcaaata ctgaaatcgc tattcatgat
     7741 tctaatatta aactatatga atctttaggc attaagcaag tttatgtaga agactttgaa
     7801 cataaatcct ttagcaaagc taaaaaagcc ttagaagaaa aagggtttaa agttgaaagt
     7861 aaggaagagt atagtgacga tattgatgag ggtgatgtga tttctcaatc tcctaaagga
     7921 aaatcagtag atgaggggtc aacgatttca tttgttgttt ctaaaggtaa aaaaagtgac
     7981 tcatcagatg tcaaaacgac aactgaatcg gtagatgtac catacactgg taaaaatgat
     8041 aagtcacaaa aagttaaagt ttatattaaa gataaagata atgacggttc aactgaaaaa
     8101 ggtagtttcg atattactag tgatcaacgt atagacattc ctttaagaat tgaaaaagga
     8161 aaaacagcaa gttatattgt taaagttgac ggtaaaactg tagctgaaaa agaagtcagc
     8221 tatgatgatg tataaatata attgaagtaa atgtaccgag gtttctattt ggaagtctcg
     8281 gtatttttat gttgagattg cggtagtttt aaaatgcttc atgtcatcat atacaggtga
     8341 gcattgtcta tcaattctgt atcactctca caatatttat atcatttgaa atgatcattt
     8401 atatattaat tttccagtct ttgatataat aaatgcagta aaattaatcg agaggtgcca
     8461 ttttgaagac aggtcgaata gtgaaatcaa ttagtggggt atatcaagta gacgttaatg
     8521 gcgaacgttt caatacaaaa ccacgaggat tatttagaaa gaaaaaattt tcaccggtag
     8581 ttggtgatat agtggaattt gaagttcaaa acattaacga aggttatatt catcaagtgt
     8641 ttgagcggga aaatgagttg aaaagaccac ctgtaagtaa tatagataca ctagtaattg
     8701 taatgagtgc tgtcgagcca aatttttcaa cgcaattatt agatcgattt ttagttattg
     8761 cacattcgta tcagttaaat gcgagaattt tggtgactaa aaaagataaa acaccaattg
     8821 aaaagcagtt cgaaataaat gagttgttga aaatatatga aaatattggc tatgagactg
     8881 aatttattgg aaatgatgat gatcgaaaaa aaattgtaga agcttggcca gctggactta
     8941 tagtacttag tggtcaatca ggtgtcggta agtccacttt cttaaatcat tatcgtccag
     9001 aacttaatct tgagacaaat gatatatcaa aatcattaaa tcgaggaaag catactacaa
     9061 gacatgtcga actattcgaa cgtcaaaacg gttatattgc agacacacct ggattcagtg
     9121 ctttagattt tgatcatata gataaagatg aaataaaaga ttattttctt gaattaaatc
     9181 gatatggtga aacatgtaag tttaggaatt gtaatcatat caaagaacct aattgtaatg
     9241 ttaagcatca attagagata gggaatattg cgcaatttag atacgaccat tatttacaac
     9301 tatttaatga aatttcaaat agaaaggtta gatattaaat gacaaaacta tatccatcat
     9361 tattatctgt tgattttttg gatttacaac atgaattaaa acgacttgaa gaagcaggcg
     9421 tcgacggagt tcattttgat gttatggatg gtcaatttgt gcctaatata tctattggtt
     9481 taccaatatt agatgcagta agaaaaggca caacattacc tatagacgta catttgatga
     9541 ttgaaaatcc agaaaagtat attgcatcat ttgcagaaca tggtgccgat atgatttcaa
     9601 ttcatgtcga atcaacgcct catattcatc gtgctattca aatgattaaa catttagata
     9661 aaaaagctgg tgtagtaatt aatcctggta caccaatatc acaaattgaa cctattttag
     9721 acattgttga ttatgtacta gtgatgacag ttaacccagg gtttggtggt caatcattta
     9781 ttgatcaatg cgtagaaaaa atagcgggtc ttaatgctat taaaatggaa cgtcaattaa
     9841 actttgatat tgaagttgat ggaggcgtaa ataccgatac agcgaaagtt tgtgttgaaa
     9901 atggtgctac aatgctagta acaggttcat ttttctttaa acaagaggat tataaaaaag
     9961 tcacacaagc attgaaaggt tgagtgtata tgcatataaa tttattatgt tctgatcgac
    10021 acttgccgca agatatttgg gccaaaagta atgaaggtaa atggggcggc gttgatagag
    10081 gtgctttgat tttattgaag catcaaatta tccctttttt ctcagtggga gactttgatt
    10141 cagtcagtaa agaagaacgc caacttctaa cagaacagtt acaaatcaaa ccagttcaag
    10201 ctgaaaaagc tgatacggat ttagctttag cggttgataa agctgttgca cttggatttg
    10261 atagtattac aatttatggt gcaacaggcg gacgattaga tcactttttt ggggcaattc
    10321 agttattatt gaaaaaagca tattataaac atgatgttca tatagaagtt atcgatcaac
    10381 aaaataaaat tgaattattg cctaaaggtc aacatacagt tgaaaaagat aagagttatc
    10441 cgtacatttc atttataccg atgactgatg atgtagaact ttctctagca ggttttaaat
    10501 ataatttagc tagacaaatg cttaatatag gttctacttt aactatttca aatgaaattg
    10561 agtctttgca agcgaaagta actgtacatg atgggttgat tttgcaaatt agaagtacag
    10621 atttaaatta agtatattca aatttgtgag gatttttaat aaagtgcatg agttatttaa
    10681 taaagtgacg tttatagata gaatttataa cataataata gatgaatcac gctgtaatta
    10741 atgaagatta tggcgtgatt tattattgaa aggaaaattg taggtcctag tacattgatt
    10801 tatgtgccaa aatcgatatt gaacccataa gtccaatttg acagagaacc attattaaac
    10861 gtattaacca aattatattg atagttcgaa tttcactatg aaccattatg tattgtttgg
    10921 taacaaaaaa agaactaaga ggttgagctc ttagttctta agtaaatgtg tcctccacat
    10981 aaggtcacat tttttatgtg caatatattt attaaactct agttacttta ccagatttta
    11041 aagcacgtgc agaaacccaa acttttttag gtttaccgtc aactaggatt ctaacttttt
    11101 gaaggttagc gttccatcta cgtttagtag agtttaaagc gtgtgaacgt ctgttaccag
    11161 tcgaagcttt acgacctgtt acgaaacatt gtttacccat atgagtacct cctttaataa
    11221 atataaatac acataactac ttatatactt aattaagata gcatagtttc atttgaaaaa
    11281 caatgaataa ttttcacata aa
//
LOCUS       DAKTLH010000080        11266 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.080, whole genome shotgun sequence.
ACCESSION   DAKTLH010000080 DAKTLH010000000
VERSION     DAKTLH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11266
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.080"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            201..1889
                     /gene="ilvD"
                     /locus_tag="O0394_001750"
     CDS             201..1889
                     /gene="ilvD"
                     /locus_tag="O0394_001750"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCY0499826.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLTLPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            1917..3686
                     /gene="ilvB"
                     /locus_tag="O0394_001751"
     CDS             1917..3686
                     /gene="ilvB"
                     /locus_tag="O0394_001751"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HCY0499827.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVR
                     ISPTEAVTPMVPSGKSNHEMEGL"
     gene            3686..3940
                     /locus_tag="O0394_001752"
     CDS             3686..3940
                     /locus_tag="O0394_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCY0499828.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            4077..5081
                     /gene="ilvC"
                     /locus_tag="O0394_001753"
     CDS             4077..5081
                     /gene="ilvC"
                     /locus_tag="O0394_001753"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCY0499829.1"
                     /translation="MTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDNGYDVV
                     IGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNLEKHNA
                     LAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQGASGQA
                     RNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGY
                     QPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVKENMKA
                     VLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKSKSIEK
                     "
     gene            5111..6640
                     /locus_tag="O0394_001754"
     CDS             5111..6640
                     /locus_tag="O0394_001754"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCY0499830.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            6643..7689
                     /gene="leuB"
                     /locus_tag="O0394_001755"
     CDS             6643..7689
                     /gene="leuB"
                     /locus_tag="O0394_001755"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCY0499831.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFVN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            7703..9073
                     /gene="leuC"
                     /locus_tag="O0394_001756"
     CDS             7703..9073
                     /gene="leuC"
                     /locus_tag="O0394_001756"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCY0499832.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            9074..9646
                     /gene="leuD"
                     /locus_tag="O0394_001757"
     CDS             9074..9646
                     /gene="leuD"
                     /locus_tag="O0394_001757"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCY0499833.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYVEIEVDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            9661..10929
                     /gene="ilvA"
                     /locus_tag="O0394_001758"
     CDS             9661..10929
                     /gene="ilvA"
                     /locus_tag="O0394_001758"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HCY0499834.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGA
                     SSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
     gene            complement(11139..11253)
                     /gene="rrf"
                     /locus_tag="O0394_001759"
     rRNA            complement(11139..11253)
                     /gene="rrf"
                     /locus_tag="O0394_001759"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3781 a         1869 c         2191 g         3425 t
ORIGIN      
        1 aatattatta ttttattata ctctttagga ctcgaacgtt agtaaatatt tactaaacgc
       61 tttaagtcct atttctgttt gaatgggact tgtaaacgtc ccaataatat tgggacgttt
      121 ttttatgttt tatctttcaa ttacttattt ttattactat aaaacatgat taatcattaa
      181 aatttacggg ggaatttact atgcgaagcg acatgatcaa aaaaggagat caccaagcac
      241 cagcaagaag tcttttacat gccacgggcg cgctaaaaag tccaactgat atgaacaaac
      301 catttgtagc tatttgtaac tcttatattg atattgttcc tggacatgtt cacttgagag
      361 agcttgcaga tatagctaaa gaagcaatta gagaagccgg tgccattcca tttgaattca
      421 atacaattgg tgttgatgat ggaatagcta tgggacatat cggaatgcga tattctctac
      481 catcacgtga aattattgca gatgcagctg aaactgtaat taacgctcat tggtttgacg
      541 gcgtatttta cattcctaat tgtgacaaga ttacacccgg tatgatttta gcagccatga
      601 ggacaaacgt accagctatc ttttgctctg gtggaccaat gaaagctggc ttatctgcac
      661 atggaaaagc attaacactt tcatcaatgt ttgaagcagt cggcgcattt aaagaaggat
      721 cgatttctaa agaagaattt ttagatatgg aacaaaatgc ctgccctact tgtggttcat
      781 gtgctgggat gtttactgca aattcaatga actgtttgat ggaagtttta ggtctaacat
      841 taccatacaa cggtactgca cttgcagtca gtgatcagcg acgagaaatg attcgccaag
      901 cagcttttaa attagttgaa aatattaaaa atgatttaaa accacgtgat atcgttactc
      961 gcgaagcaat tgatgatgca tttgcacttg atatggctat gggtggttca acaaacacgg
     1021 tactgcatac gttagccatt gccaatgaag ctggtattga ttatgactta gagcgcatta
     1081 atgctattgc caaacgcacg ccatatttat caaaaatagc acctagttca tcgtattcaa
     1141 tgcatgatgt gcatgaagct ggtggcgtcc cagcaattat taatgaattg atgaagaaag
     1201 atggcacgtt acacccagat agaatcacag ttactggcaa aacgttacgt gaaaataacg
     1261 aaggcaaaga aattaagaac tttgatgtca ttcaccctct tgatgcacca tatgatgcac
     1321 aaggcggttt atctatctta tttggtaata tcgcccctaa aggcgcagtt attaaagttg
     1381 gcggcgttga tccatctatc aaaacattta ctgggaaagc aatttgtttc aattcgcatg
     1441 atgaagctgt tgaagcaata gacaatcgta ccgttcgtgc aggccacgtc gttgtcatta
     1501 gatatgaagg acctaaaggt ggaccaggta tgcctgaaat gttagcacct acttcctcta
     1561 ttgttggtcg cggcttaggt aaagatgttg cattaattac tgatgggcgt ttttccggtg
     1621 ccacaagagg tattgcagtt ggtcatattt cccctgaagc tgcatctggt ggaccaattg
     1681 ccttaattga agatggtgat gagattacta ttgatttaac aaatcgtaca ttaaacgtaa
     1741 accagcctga agatgttcta gcgcgtcgcc gagaatcttt aacaccattt aaagcgaaag
     1801 taaaaacagg ttatctagct cgttatactg ccctagtaac tagcgcaaat acaggtggcg
     1861 tcatgcaagt ccctgagaat ttaatttaat ttatttttat attggagatg gttaaaatgt
     1921 ctaaaactca acatgaagta aaccaaaata ttgacccttt aaaaatggct gaatcacttg
     1981 aacctgaaca actaaatgaa aaaactttaa atgatatgcg ttcaggatca gaagtgctag
     2041 tagaagctct acttaaagaa aatgtggatt atttattcgg ttatcctggt ggtgccgtac
     2101 tacctttata tgacacgttt tatgatggta aaatcaaaca tattttagca agacacgaac
     2161 aaggtgctgt tcatgctgca gaaggttatg cacgtgtatc tggtaaaact ggcgtcgttg
     2221 tagttacaag cggtccaggt gcaactaatg taatgacagg tattacggat gcacattgcg
     2281 actctttacc tctagttgta ttcactggac aagttgctac accaggcatt ggtaaagatg
     2341 cattccaaga agcggatatt ctatctatga cttcaccaat tacaaaacaa aattatcaag
     2401 tgaaacgtgt tgaagatatc cctaaaatcg tacacgaagc tttccatgta gctaattctg
     2461 gacgcaaagg tcctgtagtg attgattttc caaaagatat gggtgtttta gctacaaatg
     2521 tggatttatg cgacgaaatc aatattccag gttatgaagt tgttacagaa ccagaaaata
     2581 aagacattga cactttcatc tcacttttaa aagaagcgaa aaagcctgtc gtattagccg
     2641 gcgcaggtat taatcaatca aaatcaaatc aattattaac acagtttgtt aataaacatc
     2701 aaatcccaac agttactact ttactaggtc taggtgctgt accttatgag gatacactat
     2761 ttttaggtat gggaggaatg catggttctt atgctagtaa catggcatta actgagtgtg
     2821 atttactcat taatttaggt agccgcttcg atgatagatt agcaagcaaa cctgatgcct
     2881 ttgcacctaa cgccaaaatt gtacatgtag atattgatcc ttcagaaatc aataaagtta
     2941 ttcatgtaga tttaggtatt attgcagact gtaaaagatt tttagaatgt ttaaatgata
     3001 aaaatgttga gactatagaa cacagtgact gggttaaaca ttgtcaaaat aataagcaga
     3061 aacacccatt taaacttggt gaagaagatc aagtattttg taagccacaa caaacaatcg
     3121 aatatatcgg caaaattaca aatggtgaag caattgttac tacagacgtg ggacaacatc
     3181 aaatgtgggc agctcaattt tatccattta aaaatcacgg acaatgggtt acaagcggtg
     3241 gtttaggaac aatgggattc ggtattcctt cgtcaattgg tgccaaatta gctaatcctg
     3301 ataaaacagt cgtatgtttc gtcggtgacg gtggtttcca aatgacaaac caagaaatgg
     3361 cacttttacc cgaatatggt ttagatgtca aaatcgtact aatcaataat ggaacattag
     3421 gtatggttaa acaatggcaa gataagttct ttaatcaacg cttctcacac tcagtattta
     3481 atggtcaacc tgattttatg aaaatggcag aagcatatgg cgtcaaaggt ttcttaatcg
     3541 ataagccaga acaactggaa gaacaattag atgcagcgtt tgcttatcaa ggaccagctt
     3601 taattgaggt tcgtatttcc cctactgaag ctgtaacccc aatggttccg agtggcaaat
     3661 caaatcatga aatggagggc ttataatgac aagaattctt aaattacaag ttgcggatca
     3721 agtcagcacg ctaaatcgaa ttacaagtgc ttttgttcgc ctacaatata atatcgatac
     3781 attacatgtt acacattctg aacaacctgg gatttctaac atggaaattc aagtcgatat
     3841 tcaagatgat acatcacttc atatattaat taaaaaatta aaacaacaaa ttaatgtttt
     3901 aacggttgaa tgctacgacc ttgttgataa cgaagcttaa ttttaagaca aaggcaatga
     3961 tgcgctaatt agttatagat atatcatagg ctgctagtta acatctgcca ctattacaaa
     4021 gttatatttc agaattttcg aaacacaaaa tatttaatta tttggaggaa tttattatga
     4081 caacagttta ttatgatcaa gatgtaaaaa cggacgcttt acaaggcaaa aaaattgcag
     4141 tagtaggtta tggatcacaa ggtcacgcgc atgcacaaaa cttaaaagac aatggatatg
     4201 atgtagtcat cggcattcgc ccaggtcgtt cttttgacaa agctaaagaa gatggatttg
     4261 atgtgttccc tgttgcagaa gcagttaagc aagctgatgt aattatggtg ctattacctg
     4321 atgaaattca aggtgatgta tacaaaaacg aaattgaacc aaatttagaa aaacataatg
     4381 cgcttgcatt tgctcatggc tttaacattc attttggtgt tattcaacca ccagctgatg
     4441 ttgatgtatt tttagtagct cctaaaggac cgggtcattt agttagacgt acatttgttg
     4501 aaggttctgc tgtaccatca ctatttggta ttcaacaagg cgcttcaggt caagcacgta
     4561 atattgcttt aagttatgca aaaggtattg gtgcaactcg tgcaggtgtt attgaaacaa
     4621 catttaaaga agaaactgag acagatttat ttggtgaaca agcagtactt tgcggtggtg
     4681 tatcgaaatt aattcaaagt ggctttgaaa cattagtaga agcgggttat caaccagaat
     4741 tagcttattt tgaagtatta catgaaatga aattaatcgt tgatttgatg tatgaaggcg
     4801 gtatggaaaa tgtacgttac tcaatttcaa atactgctga atttggtgac tatgtttcag
     4861 gaccacgtgt tatcacacca gatgttaaag aaaatatgaa agctgtatta actgatatcc
     4921 aaaatggtaa cttcagtaat cgctttatcg aagacaataa aaatggattc aaagaatttt
     4981 ataaattacg cgaagaacaa catggtcatc aaattgaaaa agttggtcgt gaattacgcg
     5041 aaatgatgcc ttttattaaa tctaaaagca ttgaaaaata agatagacct acaatgagga
     5101 gttgttaaat atgagtagtc atattcaaat ttttgatacg acactaagag acggtgaaca
     5161 aacaccagga gtgaatttta cttttgatga acgcttgcgt attgcattgc aattagaaaa
     5221 atggggtgta gatgttattg aagctggatt tcctgcttca agtacaggta gctttaaatc
     5281 tgttcaagca attgcacaaa cattaacaac aacggctgta tgtggtttag ctagatgtaa
     5341 aaaatctgac atcgatgctg tatatgaagc aacaaaagat gcagcgaagc cggtcgtgca
     5401 tgtttttata gcaacatcac ctattcatct tgaacataaa cttaaaatgt ctcaagaaga
     5461 cgttttagca tctattaaag aacatgtcac atacgcgaaa caattatttg acgttgttca
     5521 attttcacct gaagatgcaa cgcgtactga attaccattc ttagtgaaat gtgtacaaac
     5581 tgccgttgac gctggagcta cagttattaa tattcctgat acagtcggct acagttacca
     5641 tgatgaatat gcacatattt tcaaaacctt aacagaatct gtaacatctt caaatgaaat
     5701 tatttatagt gctcattgcc atgacgattt aggaatggct gtttcaaata gtttagctgc
     5761 aattgaaggc ggtgcgagac gaattgaagg cactgtaaat ggtattggtg aacgagcagg
     5821 taatgcagca cttgaagaag tcgcgcttgc actatacgtt cgaaatgatc attatggtgc
     5881 tcaaactgct cttaatctcg aagaaactaa aaaaacatcg gatttaattt caagatatgc
     5941 aggtattcga gtgcctagaa ataaagcaat tgttggccaa aatgcattta gtcatgaatc
     6001 aggtattcac caagatggcg tattaaaaca tcgtgaaaca tatgaaatta tgacacctca
     6061 acttgttggt gtaagcacga ctgaacttcc attaggaaaa ttatctggta aacacgcctt
     6121 ctcagagaag ttaaaagcat taggttatga cattgataaa gaagcgcaaa tagatttatt
     6181 taaacaattc aaggccattg cggacaaaaa gaaatctgtt tcagatagag atattcatgc
     6241 gattattcaa ggttctgagc atgagcatca agcactttat aaattggaaa cactgcaact
     6301 acaatatgtc tctagcggcc ttcaaagtgc tgttgttgtt gttaaagata aagagggtca
     6361 tatttaccag gattcaagta ttggtactgg ttcaatcgta gcaatttaca atgcagttga
     6421 tcgtattttc cagaaagaaa cagaattaat tgattatcgt attaattctg tcactgaagg
     6481 tactgatgcc caagcagaag tacatgtaaa tttattgatt gaaggtaaga ctgtcaatgg
     6541 ctttggtatt gatcatgata ttttacaagc ctcttgtaaa gcatacgtag aagcacatgc
     6601 taaatttgca gctgaaaatg ttgagaaggt aggtaattaa ttatgactta taacattgtt
     6661 gccctacctg gtgatggaat cggtccagaa attttgaacg gatctctatc attgcttgaa
     6721 attataagta ataaatataa ctttaattat caaatagagc accacgaatt tggtggtgcc
     6781 tctattgata cattcggcga gcctttaact gagaaaacct taaatgcgtg taaaagagca
     6841 gatgctattt tactgggtgc aatcggtgga cctaaatgga cagatcctaa caatcgacca
     6901 gaacaaggat tattaaaatt gcgtaaatcc ttaaatttat ttgtaaatat acgccccact
     6961 accgttgtca aaggcgctag ttctttatca cctttaaagg aagaacgcgt tgaaggcaca
     7021 gatttagtta tagtccgtga attgacaagt ggtatttatt ttggagaacc tagacatttt
     7081 aataatcacg aggccttaga ttctcttact tatacaagag aagaaataga acgcattgtt
     7141 cacgtagcat ttaaattggc cgcttcaaga cgaggaaaac taacatcagt tgataaagaa
     7201 aatgtattag cttctagtaa attgtggcgc aaagtcgtaa atgaagtaag tcaattatat
     7261 ccagaagtaa cagtaaatca cttacttgtt gatgcttgta gtatgcattt aatcacaaat
     7321 ccaaaacaat ttgacgtcat cgtatgtgaa aacttatttg gcgatatttt aagtgatgaa
     7381 gcttcagtga ttcctggttc acttggttta tcaccttctg ctagttttag taacgatggt
     7441 ccaagattgt atgagcctat tcatggatca gcaccagata ttgcaggtaa aaacgttgcc
     7501 aatccatttg gaatgattct atctttagcg atgtgtttac gtgaaagctt aaatcaacca
     7561 gatgctgcag atgaattaga acaacatatt tatagcatga ttgaacatgg gcaaacgaca
     7621 gcagatttag gcggcaaatt gaatactact gatattttcg aaattctatc tcaaaaattg
     7681 aatcactaag ggggagatgt aaatgggtca aacattattt gacaaggtgt ggaacagaca
     7741 tgtgttatac gggaaattgg gcgaaccgca actattatac attgatttac accttataca
     7801 tgaagttact tctcctcaag catttgaagg acttaggctt caaaacagaa aattaagacg
     7861 cccagattta acatttgcaa cactcgatca caatgttcct actattgata tattcaatat
     7921 taaagatgaa attgcaaaca aacaaatcac aacattacaa aaaaacgcca tagattttgg
     7981 ggtgcatatt tttgatatgg gttctgatga acaaggtatt gttcacatgg taggacctga
     8041 gacaggactt acacagcctg gcaagacaat cgtttgtggt gactctcaca cagcaacaca
     8101 tggagcattt ggtgctattg catttggaat tggaacaagt gaagttgaac atgttttcgc
     8161 aactcaaacg ctatggcaaa caaaacccaa aaacttaaaa atcgatatta atggtacctt
     8221 accaacaggc gtctatgcta aggacattat tctgcattta attaaaacgt atggtgttga
     8281 ctttggtaca ggctatgctt tggaatttac tggcgaaaca attaaaaacc tttcaatgga
     8341 tggtcgaatg actatttgta acatggctat cgaaggtggt gccaaatacg gcataatcca
     8401 acctgatgat ataacatttg aatatgttaa agggagacca tttgccgata acttcgctaa
     8461 atcagttgat aagtggcgtg agctatattc tgatgacgac gcgatatttg atcgtgtaat
     8521 tgaacttgat gtttcaacat tagaaccaca agtgacatgg ggaactaatc ctgaaatggg
     8581 tgttaatttc agtgaaccat tccctgaaat caatgatatc aacgatcaac gtgcgtatga
     8641 ttatatgggg ttagaaccag gtcaaaaagc tgaagacatc gacttagggt atgtttttct
     8701 cggttcatgt acaaatgcta gactatcaga tttgattgaa gctagtcata ttgttaaagg
     8761 aaataaagtt catccaaata ttacagctat tgtcgtacca ggttctcgta cagtaaaaaa
     8821 agaagcagaa aaattaggtc tagatactat ctttaaaaat gcaggatttg aatggcgtga
     8881 accaggatgt tcaatgtgtt taggcatgaa tcctgaccaa gtacctgagg gcgtacattg
     8941 tgcatctaca agtaatcgaa actttgaagg acgacaaggc aaaggtgcaa gaacacattt
     9001 agtatcccct gctatggcag cagcagcagc tattcatggt aaatttgtgg acgtaagaaa
     9061 ggtggttgtt taaatggcag caatcaaacc tattacaaca tataaaggta aaatagtccc
     9121 tctcttcaac gacaatatcg atacagacca aatcattcct aaggtacact taaagcgtat
     9181 ttcaaaaagt ggctttggtc catttgcttt tgatgaatgg cggtacttac ctgatggttc
     9241 agataatcct gatttcaatc ctaacaaacc acaatataaa ggggcttcta ttttaattac
     9301 tggagataat tttggatgtg gttcaagtcg tgaacatgct gcttgggctc ttaaggacta
     9361 tggttttcat attattattg caggaagttt cagtgacata ttttatatga attgcactaa
     9421 aaatgcgatg ttgcctatcg ttttagaaaa aagtgcccgt gaacatcttg cacaatatgt
     9481 tgaaattgag gtcgatttac caaatcaaac tgtgtcatca ccagacaagc gtttccattt
     9541 tgaaattgat gaaacttgga agaataaact tgtaaatggc ttagatgaca ttgcaatcac
     9601 cctacaatat gaatcattaa tagaaaaata tgaaaaatca ctttaaggga gttgaatatt
     9661 atgacagtca aaacaacagt ttctacgaaa gatatcgatg aggcattttt aagacttaaa
     9721 gatattgtca aagaaacacc tttacaatta gaccattact tatctcaaaa gtatgattgt
     9781 aaagtctatt taaaacgaga agatttacaa tgggtacgtt cttttaaatt aagaggtgct
     9841 tacaacgcta tttctgtttt atcagatgaa gctaaaagta aaggtattac atgtgcaagt
     9901 gcaggtaatc atgctcaagg tgttgcctat acagctaaaa aacttaattt aaacgctgtt
     9961 atctttatgc cagtcactac acctttacaa aaggtaaatc aagtaaagtt ctttggaaat
    10021 agtaacgttg aagttgtact cactggtgat acatttgatc actgtttagc tgaagcttta
    10081 acttatacaa gtgaacatca aatgaacttt atagatccat tcaataatgt tcatacaatt
    10141 tctggacaag gtacgcttgc taaagaaatg ctagaacaag caaagtctga caatgttaac
    10201 tttgattatc tatttgccgc aattggtggt ggcggtttaa tttcaggtat tagtacttac
    10261 tttaaaacct attcacctac cacgaaaatt ataggtgttg aaccttcagg tgcaagtagt
    10321 atgtatgaat ctgttgtggt aaataatcag gtagtcacat tgcctaatat cgataaattt
    10381 gtggacggtg catctgtagc tagagttggc gatattacat ttgaaattgc aaaagaaaat
    10441 gtagatgatt acgttcaagt agatgaaggt gcagtttgtt ctacgatttt agatatgtat
    10501 tcaaaacaag caattgtagc agaacctgct ggcgcattaa gtgtaagtgc gcttgaaaac
    10561 tataaagatc atattaaagg taaaacagtg gtttgtgtca ttagtggtgg taataatgat
    10621 attaatcgaa tgaaagaaat tgaagaacgt tcattactat acgaagaaat gaagcattac
    10681 tttatcttaa atttccctca acgtccaggt gcattgagag aatttgtaaa tgacgtatta
    10741 ggacctcaag acgatattac taaatttgaa tacttaaaaa aatcttctca aaatacaggt
    10801 actgtcatta ttggtattca acttaaagat catgatgatt taatacaact caaacaacgt
    10861 gtaaatcatt tcgatccttc caatatttat attaatgaaa ataagatgtt atattcattg
    10921 ttaatttaac acatagtaag aaaaacagtc ataaattgat ttctaattga aatcatctta
    10981 tgactgcttt ttattatact ttacatttct cgtttcgtca gattcaaacg ttttcacttc
    11041 gccaagccat ctttctttgt gtttgctttt attttgacgt tttagacata aaaaaagaga
    11101 ccttgcggtc tcaatgcggc tcatcgcatc cactttttgc ctggcaacgt tctactctag
    11161 cggaacgtaa gttcgactac catcgacgct aaggagctta acttctgtgt tcggcatggg
    11221 aacaggtgtg acctccttgc tatagtcacc agacatatga atgtaa
//
LOCUS       DAKTLH010000081        11265 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.081, whole genome shotgun sequence.
ACCESSION   DAKTLH010000081 DAKTLH010000000
VERSION     DAKTLH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11265
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.081"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..893
                     /gene="hlgC"
                     /locus_tag="O0394_001760"
     CDS             <1..893
                     /gene="hlgC"
                     /locus_tag="O0394_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HCY0499835.1"
                     /translation="ANPLLENAKAANDTEDIGKGSDIEIIKRTEDKTSNKWGVTQNIQ
                     FDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKAMRWPFQYNIGLKTNDKYVS
                     LINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSFNYSKSISYTQQNYVSEVEQ
                     QNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKDPRDYFVPDSELPPLVQSGF
                     NPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHYGNSYLDGHRVHNAFVNRN
                     YTVKYEVNWKTHEIKVKGQN"
     gene            895..1872
                     /gene="hlgB"
                     /locus_tag="O0394_001761"
     CDS             895..1872
                     /gene="hlgB"
                     /locus_tag="O0394_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HCY0499836.1"
                     /translation="MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKL
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(1924..2391)
                     /locus_tag="O0394_001762"
     CDS             complement(1924..2391)
                     /locus_tag="O0394_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCY0499837.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSLLLLVFGVFNPINVIISIIFRVIAIAVVYLLKINVLSLVLAS
                     VLGSLVSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPLYLTLRKRMAVLLR"
     gene            complement(2402..3094)
                     /locus_tag="O0394_001763"
     CDS             complement(2402..3094)
                     /locus_tag="O0394_001763"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HCY0499838.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFAAAKIGYHRLFDIKPVNTRYGGRIIFVDDCIDLNHYISFL
                     ESTPKQVVYETV"
     gene            complement(3105..4220)
                     /locus_tag="O0394_001764"
     CDS             complement(3105..4220)
                     /locus_tag="O0394_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HCY0499839.1"
                     /translation="MKFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEK
                     YPNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGV
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            complement(4198..5205)
                     /gene="bioB"
                     /locus_tag="O0394_001765"
     CDS             complement(4198..5205)
                     /gene="bioB"
                     /locus_tag="O0394_001765"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCY0499840.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNEVSRA
                     N"
     gene            complement(5207..6565)
                     /gene="bioA"
                     /locus_tag="O0394_001766"
     CDS             complement(5207..6565)
                     /gene="bioA"
                     /locus_tag="O0394_001766"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCY0499841.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSKKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            complement(6543..7229)
                     /gene="bioD"
                     /locus_tag="O0394_001767"
     CDS             complement(6543..7229)
                     /gene="bioD"
                     /locus_tag="O0394_001767"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HCY0499842.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTDSYIEKDNQMTIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            complement(7283..7450)
                     /locus_tag="O0394_001768"
     CDS             complement(7283..7450)
                     /locus_tag="O0394_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499843.1"
                     /translation="MQLLILTDIFYRIAVYICPNSLRSLSHFNSKVKILPKFIVILRS
                     LNSCFLEKHAP"
     gene            complement(7681..9414)
                     /locus_tag="O0394_001769"
     CDS             complement(7681..9414)
                     /locus_tag="O0394_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0499844.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            complement(9439..11202)
                     /locus_tag="O0394_001770"
     CDS             complement(9439..11202)
                     /locus_tag="O0394_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0499845.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNTYTINSIMVYIFIALIAYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATVYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
BASE COUNT         3741 a         2041 c         1526 g         3957 t
ORIGIN      
        1 ttgccaatcc gttattagaa aatgctaaag ctgctaacga tactgaagac atcggtaaag
       61 gaagcgatat agaaattatc aaaaggacag aagataaaac aagtaataaa tggggcgtga
      121 ctcaaaatat tcaatttgat tttgtaaagg ataaaaaata taacaaagat gctttgatat
      181 taaagatgca aggattcatt agctctagaa caacatatta caactataaa aaaactaatc
      241 atgttaaagc tatgcgatgg ccattccaat ataatattgg tttaaaaaca aatgataaat
      301 atgtttcttt aattaattat ttacctaaaa ataaaattga atctacaaac gtgagtcaga
      361 cattaggata caatatcggt ggtaatttcc aatcagcccc atcactcggt ggtaatggat
      421 catttaacta ttctaaatcg attagctata cacaacaaaa ttatgtaagt gaagtagaac
      481 aacaaaactc aaaaagtgtt ttatggggcg tcaaagcgaa ttcattcgcc actgaatcag
      541 gtcaaaaatc agcctttgat agcgatttat ttgtaggcta caaacctcat agtaaagatc
      601 ctagagatta tttcgttcca gacagtgagt taccacctct tgtacaaagt ggatttaacc
      661 cttcatttat cgccacagta tctcatgaaa aaggttcaag cgatacaagc gaatttgaaa
      721 ttacttacgg aagaaacatg gatgtcactc atgccattaa aagatcaacg cattatggca
      781 acagttattt agacggacat agagtccata atgcatttgt aaatagaaac tatactgtga
      841 aatacgaggt caattggaag actcatgaaa tcaaggtgaa aggacagaat tgatatgaaa
      901 atgaataaat tagtcaaatc atccgttgct acatctatgg cattattatt actttctggt
      961 actgctaatg ctgaaggtaa aataacacca gtcagcgtaa aaaaagtcga tgacaaagtt
     1021 actttataca aaacaacagc cacagcagat tctgataaat ttaaaatttc acagatttta
     1081 acatttaatt tcatcaaaga taaaagttat gataaagata ctttagtact taaagctact
     1141 gggaatatta actcaggctt tgtgaaacct aatcctaatg actatgactt ttcaaaatta
     1201 tattggggag ctaaatacaa tgtatctata agctcacaat ctaatgattc agtaaacgtc
     1261 gttgattatg caccaaaaaa tcaaaatgaa gagtttcaag ttcaaaatac tttaggctat
     1321 acatttggtg gtgacattag tatctctaat ggtttatctg gtggacttaa tggaaataca
     1381 gctttttctg aaacaattaa ttataaacaa gaaagttaca gaacaacatt aagtcgcaac
     1441 acaaattata aaaatgttgg ctggggagtt gaagcacata aaattatgaa taatggttgg
     1501 ggaccttatg gaagagatag cttccaccca acatatggta atgaactctt cttagctggc
     1561 agacaaagca gtgcatacgc tggccaaaac ttcatagcgc aacaccaaat gccattatta
     1621 tctagaagta acttcaatcc agaattttta agcgtactat cacacagaca agatggcgct
     1681 aaaaaatcta aaattacagt aacttatcaa cgtgaaatgg atttatacca aattcgttgg
     1741 aatggcttct actgggcagg cgcaaattat aaaaacttta aaactagaac atttaaatca
     1801 acatatgaaa ttgattggga aaatcacaaa gtgaaattgt tagatacaaa agaaactgaa
     1861 aacaataaat agctagtaaa acacggtcgc caacagtaat tgtgacgacc gtgttttgat
     1921 ttattatctt agtaatactg ccattctttt tctcaatgtg agatataaag gaatagctac
     1981 aattaaagtg aatattacgc ctggaatcgc gtttaacaac actacccaca caggtaaatt
     2041 taaaataata gataatagta gcctagatac caaactgcct aatacacttg ctaaaactaa
     2101 tgatagtaca tttattttca ataaataaac aactgcaata gctataactc taaatataat
     2161 agagataatc acattaattg gattaaatac gccaaatact agtaataata ggctagataa
     2221 taatccacct aaaaagtact ttttaattcc aaagaaagct aatatcaata atgctgccgg
     2281 tgcagataat tgaaaatcta atcctggtat aatggacggt attttcaaaa ctgccaaaat
     2341 ggttaaaatc gcagcaatga cactaatttg agtaatatct tttgatgtca tactaaaacc
     2401 cctataccgt ttcataaaca acttgcttcg gtgtgctttc taaaaatgat atgtaatgat
     2461 ttaaatcaat acaatcgtcc acaaatatta ttctgcctcc atatctcgta ttaactggtt
     2521 taatatcaaa taatcgatgg taaccaattt tagcagcggc aaaataacct gtcgtatacg
     2581 ttaagtcatc ggacacgcaa agttctcctt tgacatacgg atgcgcattg atacaactag
     2641 caattgctaa ggcatcagtc actctttcat taagatcacc tttattattt atatcttcaa
     2701 acgaaaaatg tgttgcccta atcccccttt gtccaaatga atctaaacgt ttaccagata
     2761 tagcagatag aataatagct cctgtataaa ccgtttcatt tttaatatat gtcatccctt
     2821 gatttagcgc ttgttcagtg acaccacatt cttgtgttaa atgttggaga tttgctttat
     2881 catcctcaat aatttgtaat gcttttattt gttgaatcgg ttccatgatt ttttgtattt
     2941 ttatattgag aaaatccggt tgcccatttt catgaaaaaa acctttatta taaaaccttt
     3001 gtaccgtctg ttctattttg tcaaactcac aaatcgtttc agcaccacta atatgaacat
     3061 cttgattgct agaacgcatt tttatactat acatgacgat cacctcaatc ttcttgatgc
     3121 aaaatttcaa acaacctatc tatatcttgt tcagtatgaa aatacgacaa tgatattctt
     3181 aacattggct tagtcacagt tggatacctt aaataacttg taaacacatg atgctttaat
     3241 aatgtttgat gaatgttctc agccgcttct atgtcatcaa actcaataaa cttaatcggc
     3301 gagtttgaac tattataatt aacattgagt gcttttaact tttggttaaa atatttactt
     3361 aaactattta atttagtgcg tctatcatca gcatttatta acttttcaat gtttcttttt
     3421 ataaaataca aattataaat tggcaaacta cttgagtaga tgagtggtct accgtgatta
     3481 attaacatat ccttcacatc atttgaactt aaaatcacac ccccgtatgc accacatgct
     3541 ttagataaac tagaagtgag tatatctaca ccttgataat tcgagtaatt ctctattcca
     3601 aaactatgtg aaacatcgag tatcagtgtt gcgttatatt tatgctttaa tgagactaat
     3661 tgaccaatat ccacaacgtc gccattcgtt gaaaatacac tatcagatat gattattttt
     3721 ggtatatttt gattagggta tttctctaac cttttttcta aatcagcaat atctaaatgc
     3781 ttatatatca ctttttctaa accacttaac ttaataccgt caataatact cgcatgattt
     3841 tcttgatctg aaaacacgac acaatttgta tttttgaaaa tattaaataa cgccaaatta
     3901 gcatcataac cactatttaa gatagtacat gcactatatc cgagccaacc tgctaacatt
     3961 gtttcaattt cttcataagc tgtcgaactt ccactaatta atcttgaact tgataagtga
     4021 taactatact tccgcataaa tctttcgaaa tcatccttat caaacgctat ttgacctaat
     4081 cctaaataat cattagatgt atagttcgta catctcttat tttctacttc aatatactgt
     4141 ctatctatat accctaccga tttaagcgac cgatataacc ctttctgttg taataaatca
     4201 atttgctctt gaaacttcat tcttgttttc cttattttca caagtgtcat aatcaatttc
     4261 aaagcctaaa tcattaatca tatcgtagtc taattggttc ggttgcccac cagtaattag
     4321 ataatcaccg acaaatattg aattcgccgc ttttaatgct aatggctgta acgaacgtaa
     4381 gttgacctct cttcctccag caatacgaat ttctttcgta ggattgatta atcggaataa
     4441 tgctacgatt cttaaacatt tcattggtgt taaatcatcc atgcttccaa actttgtgcc
     4501 tttgattgga tgcaaaaaat taatcggaat actgtcggca tccatttctt ttaaagcaaa
     4561 tgccatatca acaatatctt gattagattc tcccatacca caaatcacgc cagaacatgg
     4621 tgatatatta ttcgctttca ttagttctat cgtatctgtt ctatctttat aactatgcgt
     4681 tgtcacgacg ttatcatggt aattttcact tgtattaata ttgtggttat atctgtctac
     4741 accagctgac ttaagtttct tagcttgttc gtcattcgtt aatcctaaac atgcacagat
     4801 ttttagttgc gggtgttgag atttaatcgt tcttacagta ttactaatat gatcaacttc
     4861 tttatcgctc ggtcctctac cactcataac aatacaatat gttccaatat gattatcatg
     4921 tgccaccttt gctccatcga taatttgttc ctctggaatt aaagcatatc gctgtttttg
     4981 tttaatatct cgtgattgtc cacagtaccc acaattttca ggacatatac cacttttagc
     5041 atttaaaatc atgtttaatt ttactttttt accaaaataa tgttttctta aaatgtacgc
     5101 ctcatttaat aaatctaagg tatcaatatt agtatcctca taaattttca atacagtctc
     5161 ttttgttaat tgttcccctt gtaatatgcg tttagccaaa ttcatattaa cacttcctat
     5221 ctaaaatgtt atgtactgcc tttttaaaaa tacgtaccat cgttttcact tctttatttg
     5281 acatacttaa cactggcaca aacgtaatga cattttctaa atttctaatc attagcccgt
     5341 tttctttaca attacgtaca atacgttcaa ctttttcaat ttctaacggc gttttactat
     5401 ctttatctgt aactaattca actccaaaca ttaagcctcg tcctcttaca tcaccaacat
     5461 ttcgatgacg cttcagcgca tgtaactgtt tctcaagtgt tgatgatgtc gtttcaatat
     5521 gtgacaataa cttacgtttt tcataaagtc ttatattttc taatgcaacc gtacaaacga
     5581 tttgatttcc ggtgtatgta tgaccatgga aaaaggtatt cacaccatgc gaatcactta
     5641 aaaatgcatt gtatattttt ttagatgtca atgtagctgc aagtggtaag tagccaccag
     5701 taatcgcctt acctaaacac ataatatccg gttgaacatc ttcatgattg catgcaaaca
     5761 tctttccagt tctcccaaaa ccaactgcta cctcatcaca aattaataag acatcgtatt
     5821 ttttgcacaa tttctcgact tctttcaaaa agcctttagg atgaacaaac aagcctgtcg
     5881 caccttgaat caacggttcc aatataaacc ctgcgatttc atcatttctt tcaattagaa
     5941 tttgctctat attcgttaaa atagctgtca tcatttcatt ttcagtatcg taattactgc
     6001 gatagaaaga tgggctttct acttgaatat tctcaaatat taagtcttta aatattttat
     6061 gaaaggtctt gataccacca acacttaccg caccaatcgt atccccatga taaccgtgat
     6121 ttagcgttat aaacttgttt ttcttggcat atttttctct atcaatattt ttccaatact
     6181 gatatgccat ctttattgcg atttcaacag acgcactgcc tgtatcagaa taaaatactt
     6241 ttcttagatt acttggcgtg atttcgatta atttttccgc aagttctatt gacggaatat
     6301 ttgatgatcc tagcagcgta gaatgggcaa ttttattgag ttgcttttta attaccttat
     6361 ttaagtattt gttattatga ccatgcacat tgacccacaa cgatgcataa ccatctaaat
     6421 atttattgcc attcgtatcg taaaggtaac taccctttcc tttttcaatg atgattgctt
     6481 cttctttgct atatacaccc atttgtgtaa atggatgcca aacatattct gagtcttttt
     6541 gtttaagttg ttgtgtgtaa ttcattttta actcctatta attgttttaa aaatgcttct
     6601 gagaaatttt catacgtggc atgttcttca aatgtataga ctgttttatt tgttaatttt
     6661 ccaatcgtca tttgattgtc tttttcaata tagctgtctg tatagcgatt cattattaaa
     6721 aaattactcg ccgatacatt ctgattaaca taatcttggt gaacaatggc atcgctaata
     6781 gcacctaatt ttgatggcaa cacactgatg acacaatctg cacaatcatt gattagatct
     6841 ttagtcatgt agaaatcatc tgtaccttca tatattggta cggcaattcc cccagcaccc
     6901 tcaattaaga taaaatcaaa ttccttatct aaaactttta ccttatctaa tacacgctgc
     6961 ttatttagaa aaatttgatc tgtcatttta aatgcaaggt gtggtgatac aggttgctta
     7021 aaagtataaa gtgacgttat gtcatagctt aaatcacatt catttttaaa tacttctaaa
     7081 tctggaaacg tcccgtcttg gcgttcctca gtttgaaatg gtttaaaaat acaaactcga
     7141 tgaccacgtg ttttcaaagc atggtataaa tgctttgtaa cataggtttt gcctacatca
     7201 gtattcgtac ttgtaataaa aatcctcata aaaataacca cctcaatcca aatgtaaact
     7261 tttataatta ataagtttac atttaaggcg catgtttttc aagaaaacat gaatttaaac
     7321 ttctcagtat aactataaat tttggcaaaa ttttgacctt gctattgaaa tgactaagac
     7381 ttcttaagga attcgggcag atataaacag cgattcgata aaatatgtca gttaaaatta
     7441 ataattgcat tatttcttaa taaaaagaca tcctagcttt accatcttgt gaaacaatcc
     7501 attaattgta atattacgtt gagcgccttg ttcatcaatg ctataattag ttacataata
     7561 aattgaacat ctaaatacac caaatcccct cactattgcc ctagtgaggg gatttatttt
     7621 gctgttgatt atctatcacc ctttttattt ccaaattctg attccatatt aaactattat
     7681 ttataatttc cagcctgctg atttactttt tattcttata aagtcttgat acattcctgg
     7741 ttttcgaatt aattcatcat gactaccttt ttgaattatt tcaccttcat tgagcactat
     7801 aatctgatct gcatttttaa tagtttcaag tttatgtgca attgtaatta ctgttttgcc
     7861 tttacttaat tcattaattg ccgtttgaat cagctgttca ttttcagggt caatacttgc
     7921 agttgcttca tctaaaataa ttattggtgc atcttttaat atcgccctag caatagaaat
     7981 cctttgcttt tctccgccag ataaattact acctttttca tttagcattg tttgatatcc
     8041 ctcaggtaat gacatgatga agtcgtggca acatgcttgc ttcgctgcac gaataatttc
     8101 ttctttcgtt gcacctggat tgccaaacaa tatgttattt tcaatcgtat cattaaataa
     8161 atacaccttt tgaaatactg cactaatttt cgacattaac gtacttaatg tcatatcttt
     8221 aatatcaaca ccatcgatgc gaatatttcc atcatcgata tcataaaagc gcaagagtaa
     8281 gtgacataat gtagatttcc cacttcctga aggtccaatt attgctgttg atgtttgtgt
     8341 aggtatctca aaattcacat tctttatcac ttgtttatca tcatatgaaa agttcacgtt
     8401 ttgaaaagcg atattttcat ttttaatcgt caaatttttt ccattctcat ccagttctgg
     8461 agcatttttt atcttttcaa tatcatctat cgtcatatct atcatttcaa gtacgtgtgc
     8521 agcactatta atattttcaa cactatcaaa tatcacaaat gaaaaaattg aaatcataat
     8581 aagggtaggt aaatcaatac tatgattcat atataataag catgcaacta atacaatcat
     8641 tattgaaaca acttttaaac taagtaaatg caataaattg aatgggatat attgcatttc
     8701 tatttttgta tttatacgtt tactttcatt gactgcttgg ttaaaacttt taagactcgt
     8761 attttctttc gcgaatgatt ttattacttg aataccacga ataacctcca ataccttttc
     8821 cactaattgg ttttgtacat tatgatacgc tggcgcattt tgtcgactct ttctttctaa
     8881 taattgaatc gcaaaaaatg atagtaatac gccaatgcat gctaataatg atacttgcca
     8941 cgaaactaca agtagagaca atatgagtac tgtaattaat atgtatccat taacaacaac
     9001 gtccaccatt ttcatagcaa agttttctaa aaaggttaaa tctgttgtta ctattgttgt
     9061 taactcattt gaatgatgcg aattaaaata acctaacctt acatttttca atttatcccc
     9121 tatatctaaa cgttctttcg cactcatttc ataagcgatg ctctcatggc ttttgctttt
     9181 gaaatatgct gtaataaatc gtccaatcac taataaaacc atgataatta caacattcaa
     9241 tatatctttc atataaatag gtttatgaga tagcacatta ttaaatattt ttgcagctaa
     9301 aaagataggt aacgcaataa aaatagcatt taaaaatgac atgctaaatc ctaaaatcat
     9361 tcttgcttta tatggtctta tccagtttaa aattttaaat gtaatttgaa acatatttga
     9421 cctccttttc tcattagttt aattatgtcc agtattgatt ccccaatctt ttgtatgcat
     9481 gtgcgtatcc cacattttct tataattacc gtttaatttt agcaataagt gatgtgaccc
     9541 tttttctaaa atttgttgtt tacctaaaac aataatttga tctgcatgtt gaatcgtaga
     9601 taaccgatgt gcaataacaa tcaatgtttt atcctgcgtc aacacattta atgcttcttg
     9661 aattttttgt tcgttgtctg gatcaacgta aacagtcgct tcatctaata caatgatagg
     9721 cgcatctttt aatatcattc ttgcaatagt gactcgttgt ttttcaccac cagacaattt
     9781 atctcccact gtaccaacat tcgtatcata tccatctggc aacttttcaa taaattcatg
     9841 acattgtgct aacttggcag ctttttcaac tgcctcatcc gtagcttctg gattgccaag
     9901 tttaatattt tctttaaaag taagatttaa taaaaagtta tcttgtccaa caaaaccaac
     9961 taaatcgtta agttgtttcg attcaatatc ttttatattt ataccgccaa tcgtaatttc
    10021 acctgaagtc acatcccagt atcgtgatat aagcttggca atggttgact taccgctacc
    10081 agatgccccg acgatagctg tgaaattatt ttctggtact gtaaatgata aatgcttaaa
    10141 gaccagatcg tccttatctt tgttatatga aaatccaaca ttattaaaag caatttcata
    10201 atgttgaggc ttcttaaatt ttgttgacaa tactaactct tctagactta atatttgatt
    10261 cacttcagtt aatgcgtatt gtatagactt taaatgattt acataattag taaaattctt
    10321 aatcggtgct actacaccta aagataatac gatgcatagg aaaaattccg catagtttaa
    10381 ttggttgata gatatcaaat acatgccgac cggtaaaatc cctaaaaatg ttgaaggtaa
    10441 tacactagct cctaaattca tataccccca tgtattttta aaccaattca aagtgtgaat
    10501 cttataatta tctactgcat ctttatattt tttatatgaa ctttgagatt gattaaatgt
    10561 tttaatcact tcaatgcctt cgataaactc tacaatcgcg ctattcatat aattattcga
    10621 tttcatttgt tcagcatatg tctcattaaa tccagacata acttttttaa aagcgaaaat
    10681 tgaaattggt atcgttacta ataaggcact agccatacgc caatcaatga gcattatgta
    10741 taaaaagata gcagctgaca aaagtaagtt tcctataact tcaggaatca tatgtgctaa
    10801 aggtaattct attgtttcaa ccttatcgac aaatatattt tttaattcac ctattttctt
    10861 agattccact acgcctaaag ggagacgcat taatttttga gctaattttt tacgaatttc
    10921 agataaaatt tcatatgccg taatatgtga tagcatcgtt gacgctccaa aacaacacac
    10981 ttgtgaaata taagcgatta aagcaataaa gatataaacc ataatcgaat taatcgtata
    11041 tgtattgtta atcatcatta aaataatttt aaatactgcc caataaggaa ctaatccaga
    11101 aaagacactg atgatagaca acaaaattga taacataatt ttccatttac tatcagaagc
    11161 aaatccgctg agtatcttta tccaatttgt ctgattgttc atcgtatatg taactccttt
    11221 caattaataa tctaaattaa gccgcttata ttatttattt cactg
//
LOCUS       DAKTLH010000082        11215 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.082, whole genome shotgun sequence.
ACCESSION   DAKTLH010000082 DAKTLH010000000
VERSION     DAKTLH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11215
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.082"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            170..1651
                     /locus_tag="O0394_001771"
     CDS             170..1651
                     /locus_tag="O0394_001771"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCY0499846.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVD"
     gene            1635..1895
                     /locus_tag="O0394_001772"
     CDS             1635..1895
                     /locus_tag="O0394_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HCY0499847.1"
                     /translation="MALLINKRFIDEGKTIDVYLFEALNNQIIIAIPDWFWSYQMAMT
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            1895..3457
                     /locus_tag="O0394_001773"
     CDS             1895..3457
                     /locus_tag="O0394_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCY0499848.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
     gene            3759..4562
                     /locus_tag="O0394_001774"
     CDS             3759..4562
                     /locus_tag="O0394_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCY0499849.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            4781..7105
                     /locus_tag="O0394_001775"
     CDS             4781..7105
                     /locus_tag="O0394_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCY0499850.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYN
                     RDHYLTDDVSKKQNSLDSVDQDTVKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHIPSISDDKDNVMRENHIVDDNPDNDINTPSLSKTDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSTASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSKLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            7122..8480
                     /locus_tag="O0394_001776"
     CDS             7122..8480
                     /locus_tag="O0394_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCY0499851.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMILMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            8619..10130
                     /locus_tag="O0394_001777"
     CDS             8619..10130
                     /locus_tag="O0394_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCY0499852.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSDNFIVKNR"
     gene            complement(10310..10398)
                     /locus_tag="O0394_001778"
     tRNA            complement(10310..10398)
                     /locus_tag="O0394_001778"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10361..10363),aa:Ser,seq:gct)
     gene            complement(10401..10475)
                     /locus_tag="O0394_001779"
     tRNA            complement(10401..10475)
                     /locus_tag="O0394_001779"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10441..10443),aa:Asn,seq:gtt)
     gene            complement(10617..11186)
                     /locus_tag="O0394_001780"
     CDS             complement(10617..11186)
                     /locus_tag="O0394_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCY0499853.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
BASE COUNT         4008 a         1521 c         2143 g         3543 t
ORIGIN      
        1 tttaagatga gccataattg taaaactaat tcataatacc gtatgtttta tttttaatag
       61 tagaaattag aaaatgctga ttagtaggat ataacagtga aattataaat ttattaacat
      121 caacaaaacg tgtataataa acatattgta gaaaaaggag cggttcagtt tggatgcaag
      181 tacgttgttt aagaaagtaa aagtaaagcg tgtattgggt tctttagaac aacaaataga
      241 tgatatcact actgattcac gtacagcgag agaaggtagc atttttgtcg cttcagttgg
      301 atatactgta gacagtcata agttctgtca aaatgtagct gatcaagggt gtaagttggt
      361 agtggtcaat aaagaacaat cattaccagc taacgtaaca caagtggttg tgccggacac
      421 attaagagta gctagtattc tagcacacac attatatgat tatccgagtc atcagttagt
      481 gacatttggt gtaacgggta caaatggtaa aacttctatt gcgacgatga ttcatttaat
      541 tcaaagaaag ttacaaaaaa atagtgcata tttaggaact aatggtttcc aaattaatga
      601 aacaaagaca aaaggtgcaa atacgacacc agaaacagtt tctttaacta agaaaattaa
      661 agaagcagtt gatgcaggcg ctgaatctat gacattagaa gtatcaagcc atggcttagt
      721 attaggacga ctgcgaggcg ttgaatttga cgttgcaata ttttcaaatt taacacaaga
      781 ccatttagat tttcatggca caatggaagc atacggacac gcgaagtctt tattgtttag
      841 tcaattaggt gaagatttgt cgaaagaaaa gtatgtcgtg ttaaacaatg acgattcatt
      901 ttctgagtat ttaagaacag tgacgcctta tgaagtattt agttatggaa ttgatgagga
      961 agcccaattt atggctaaaa atattcaaga atctttacaa ggtgtcagct ttgattttgt
     1021 aacgcctttt ggaacttacc cagtaaaatc gccttatgtt ggtaagttta atatttctaa
     1081 tattatggcg gcaatgattg cggtgtggag taaaggtaca tctttagaaa cgattattaa
     1141 agctgttgaa aatttagaac ctgttgaagg gcgattagaa gttttagatc cttcgttacc
     1201 tattgattta attatcgatt atgcacatac agctgatggt atgaacaaat taatcgatgc
     1261 agtacagcct tttgtaaagc aaaagttgat atttttagtt ggtatggcag gcgaacgtga
     1321 tttaactaaa acgcctgaaa tggggcgagt tgcctgtcgt gcagattatg tcattttcac
     1381 accggataat ccggcaaatg atgacccgaa aatgttaacg gcagaattag ccaaaggtgc
     1441 aacacatcaa aactatattg aatttgatga tcgtgcagaa gggataaaac atgcaattga
     1501 catagctgag cctggggata ctgtcgtttt agcatcaaaa ggaagagaac catatcaaat
     1561 catgccaggg catattaagg tgccacatcg agatgattta attggccttg aagcagctta
     1621 caaaaagttc ggtggtggcc ctgttgatta ataaaagatt tattgatgaa ggtaaaacta
     1681 ttgatgttta tttattcgaa gcattaaata accagataat cattgctata ccagattggt
     1741 tttggtcata tcagatggca atgacattag atgaagaaac ttgttttgaa gcaatactca
     1801 tgcaattgtt tgtttttaaa gaagaggaag aggcagaatc gattgcatca caactaacag
     1861 attggataga aacatataaa aaggagaaag actaatgaac ttaaagcaag aagttgagtc
     1921 tagaaagact tttgcgatta tttcacatcc cgatgcaggg aaaacaacgt taactgaaaa
     1981 actattgtac ttcagtggtg ctattcgtga agcgggtaca gttaaaggga agaagactgg
     2041 taaatttgcg acaagtgact ggatgaaagt tgaacaagag cgtggtattt ctgtaactag
     2101 ttcagtaatg caatttgatt acgatgatta taaaatcaat atcttagata caccaggaca
     2161 tgaagacttt tcagaagata cgtatagaac attaatggca gttgacagtg ctgtcatggt
     2221 catagactgt gcaaaaggta ttgaaccaca aacattgaag ttatttaaag tttgtaaaat
     2281 gcgtggtatt ccaatcttta cattcattaa taaattagac cgagtaggta aagaaccatt
     2341 tgaattatta gatgaaatcg aagagacatt aaatattgaa acatacccta tgaattggcc
     2401 aattggtatg ggacaaagtt tctttggcat cattgataga aagtctaaaa caattgaacc
     2461 atttagagat gaagaaaata tattacattt gaatgatgat tttgagttgg aagaagatca
     2521 tgcaattaca aatgatagtg attttgaaca agcgattgaa gaattaatgt tggttgaaga
     2581 agcgggtgaa gcctttgata atgacgcgct gttgagtgga gacttaacac ctgtattttt
     2641 cggttcagct ttagctaact ttggtgtaca aaatttctta aatgcatatg ttgattttgc
     2701 gccaatgcca aatgcgagac aaacaaaaga agacgttgaa gtaagcccgt ttgatgattc
     2761 attttcagga tttatcttta aaattcaagc caacatggac cctaaacacc gtgatagaat
     2821 tgcctttatg cgtgtcgtta gtggtgcatt tgaacgtggt atggatgtta ctttgcaacg
     2881 tactaataaa aagcaaaaga tcacacgttc aacgtcattt atggcagacg ataaagaaac
     2941 tgtgaatcat gctgtagcag gcgatatcat tggactatat gatactggta attatcaaat
     3001 tggagatact ttagttggtg gaaaacaaac ctacagtttc caagatttac cacaatttac
     3061 gccagaaatt tttatgaaag tttctgctaa aaacgtcatg aaacagaagc atttccataa
     3121 aggtattgaa caattagtac aagaaggtgc gattcaatac tataaaacat tacacacaaa
     3181 ccaaattatt ttaggtgctg ttggtcagtt acaatttgaa gttttcgaac atagaatgaa
     3241 aaacgaatat aatgttgatg ttgttatgga gccagtaggc cgtaaaattg cacgttggat
     3301 tgaaaatgaa gaccaaatta cagataagat gaacacatca agatcgattt tagtgaaaga
     3361 tagatatgac gatttagtat tcttatttga aaatgaattt gcaacaagat ggtttgaaga
     3421 gaaattccct gaaattaaat tgtatagttt actttaacag ctcaattgta taatcgaatt
     3481 tgttacatta aaaataattg tttcgttgaa gaaaaataaa ttgtatattt taaaagaaaa
     3541 aggtatacta tgatgtatca aatgaataac ctatggcatt ttgtcagagg ggagtaactt
     3601 aagaatcatg accgtataaa tgattcgaca ctttatcgtc attacgaaga tatcttccgg
     3661 taaagtgggc aatttaaatt gcttagtgag acctttgcta tttatttagc ataggtcttt
     3721 ttgtttgtac ttaacttatt tatttaaagg agttgtacat gttaatggat ccaagtttga
     3781 tcttacctta tttatgggta cttgtcgttt tagtattttt agaaggctta ttagcagcag
     3841 ataacgcgat tgttatggct gtaatggtta agcacttacc acccgaacaa cgtaaaaaag
     3901 ctttgtttta cggtttgtta ggtgcatttg tatttagatt tttagcatta ttcttaatta
     3961 gtattatcgc gaacttttgg tttattcaag ctgcaggagc ggtttactta atttatatgt
     4021 caatcaaaaa tctgtggcag ttctttaaac acccagaaat tgaaagtcct gaagctggag
     4081 atgatcatca ttatgatgaa tctggtgaag agattaaagc aagtaacaaa tcattctggg
     4141 gaactgtgtt gaaaatagaa tttgcagata tcgcatttgc cattgattct atgcttgctg
     4201 ctttagctat tgctgtaaca cttcctaaag ttggtattca ctttggtggt atggacttag
     4261 gtcagttcgt agtcatgttc ctaggtggaa tgattggtgt tattctaatg cgttatgcag
     4321 caacatggtt tgtagagcta ttaaacaaat atccaggact tgaaggtgca gccttcgcga
     4381 tcgttggttg ggtaggtgtt aaattagttg tcatggtatt agcgcaccca gacatcgctg
     4441 tattgcctga gcacttccca catggcgtat tatggcaatc tattttctgg acagtactaa
     4501 ttggattagt aattatcggt tggttaggtt cagttgttaa aaataaaaaa tcgcataaat
     4561 aattgatgtg aagcggacaa tcttaattta gtttaaggtt gtcctttttc atttaattga
     4621 gtgatttatg aaaaatggat tttgaagaat gtgaatcaaa agatgcgata tagtattaag
     4681 aaaatgtgcc ttttatattt agcatttttt caatagaaat tatatagatt ttaaagcaaa
     4741 ttaggtgtta atgtgtcata atgataagtg attttattga atggagtgga cattagtgga
     4801 tattggtaaa aaacatgtaa ttcctaaaag tcagtaccga cgtaagcgtc gtgaattctt
     4861 ccacaacgaa gacagagaag aaaatttaaa tcaacatcaa gataaacaaa atatagataa
     4921 tacaacatca aaaaaagcag ataagcaaat acataaagat tcaattgata agcacgaacg
     4981 ttttaaaaat agtttatcat cgcatttaga acagagaaac cgtgatgtta atgagaataa
     5041 agctgaagaa agtaaaagta atcaggatag taagtcagca tataacagag atcattattt
     5101 aacagacgat gtatctaaaa aacaaaattc attagattca gtggaccaag atacagtgaa
     5161 atcaaaatat tatgagcaaa attctgaagc gactttatca actaaatcaa ccgataaagt
     5221 agaatcaact gaaatgagaa agctaagttc agataaaaac aaagttggtc atgaagagca
     5281 acatgtactt tctaaacctt cagaacatga taaagagact agaattgatt ctgagtcttc
     5341 aagaactgat tcagacagct cgatgcagac agagaaaata aaaaaagaca gttcagatgg
     5401 aaataaaagt agtaatctga aatctgaagt aatatcagac aaatcaaata cagtaccaaa
     5461 attgtcggaa tctgatgatg aagtaaataa tcagaagcca ttaactttac cggaagaaca
     5521 gaaattgaaa agacagcaaa gtcaaaatga gcaaacaaaa acctatacat atggtgatag
     5581 cgaacaaaat gacaagtcta atcatgaaaa tgatttaagt catcatatac catcgataag
     5641 tgatgataaa gataacgtca tgagagaaaa tcatattgtt gacgataatc ctgataatga
     5701 tatcaataca ccatcattat caaaaacaga tgacgatcga aaacttgatg aaaaaattca
     5761 tgttgaagat aaacataaac aaaatgcaga ctcgtctgaa acggtgggat atcaaagtca
     5821 gtcaactgca tctcatcgta gcactgaaaa aagaaatatt tctattaatg accatgataa
     5881 attaaacggt caaaaaacaa atacaaagac atcggcaaat aataatcaaa aaaaggctac
     5941 atcaaaattg aacaaagggc gcgctacgaa taataattat agtgacattt tgaaaaagtt
     6001 ttggatgatg tattggccta aattagttat tctaatgggt attattattc taattgttat
     6061 tttgaatgcc atttttaata atgtgaacaa aaatgatcgc atgaatgata ataatgatgc
     6121 agatgctcaa aaatatacga caacgatgaa aaatgccaat aacacagtta aatcggtcgt
     6181 tacagttgaa aatgaaacat caaaagattc atcattacct aaagataaag catctcaaga
     6241 cgaagtggga tcaggtgttg tatataaaaa atctggagat acgttatata ttgttacgaa
     6301 tgcacacgtt gtcggtgata aagaaaatca aaaaataact ttctcgaata ataaaagtgt
     6361 tgttgggaaa gtgcttggta aagataaatg gtcagattta gctgttgtta aagcaacttc
     6421 ttcagacagt tcagtgaaag agatagctat tggagattca aataatttag tgttaggaga
     6481 gccaatatta gtcgtaggta atccacttgg tgtagacttt aaaggcactg tgacagaagg
     6541 tattatttca ggtctgaaca gaaatgttcc tattgatttc gataaagata ataaatatga
     6601 tatgttgatg aaagctttcc aaattgatgc atcagtaaat ccaggtaact cgggtggtgc
     6661 tgtcgtcaat agagaaggaa aattaatagg tgtagttgca gctaaaatta gtatgccaaa
     6721 cgttgaaaat atgtcatttg caatacctgt taatgaagta caaaagattg taaaagattt
     6781 agaaacaaaa ggtaaaattg actatcccga tgtaggtgtt aaaatgaaga atattgccag
     6841 tctaaatagt tttgaaagac aagcagttaa attgccagga aaagttaaga acggtgttgt
     6901 tgtagatcaa gttgacaaca atggtttagc agatcaatct ggtctgaaaa aaggtgatgt
     6961 aattactgaa ttagatggca aacttttaga agatgattta cgctttaggc agattatatt
     7021 tagtcataaa gatgacttga aatcaattac agcgaagatt tatagagatg gtaaagagaa
     7081 agaaattaat attaaactaa aataatgttg aggtgaacaa cgtgtcaatt tttagccagt
     7141 ttttaaaaag atcaagccct caacaaggta ttgtattgta ctatatcgtc gcaattgtca
     7201 ttgcattttt attattaaac ttaccgtatg ttcataaacc aggtgtagaa gtaaatccaa
     7261 ttgacacatt atttgttgcc gtatccggaa ttagtgttac tggattgtct ccgataagta
     7321 ttgtcgatac ctattctaca tttggacaat taattatcct cgtgatatta aatattggtg
     7381 gaattggcgt catggcaatt ggtacgatgt tatgggtggt actaggtaaa catattggaa
     7441 ttagagaacg tcagttaatt atgttagata ataacaaaaa cacaatgagt ggtaccgtca
     7501 aattgattat tgatattgta aaatcaatat ttgtaatcga actcgtagga gccatgttat
     7561 tagcatttta cttttatcga gataatccag atttaaaata tgcaatcatg caaggtgttt
     7621 ttgtttctat ttctgccact accaatggtg gattagatat tacaggtaag tcattaattc
     7681 cttatgcaca tgattatttt gtacaagcga tagttatatt tttaataatt ttaggatcaa
     7741 tcggcttccc agtattatta gaagttaaag cttatattca aaatagggtt actaatttta
     7801 gattttcatt atttactaaa attacgacat caacatattt attcctattt attgttgggg
     7861 tattagccat tctattattt gaacataacc atgcgttcaa aggtttaagt tggcatcaat
     7921 cgttattcta ttcgctgttt caatcagcga ctacaagaag tgcgggtctt caaacaattg
     7981 atgtgacaac actaagtgac cccactaata ttatcatggg tattttaatg tttataggat
     8041 cttcgccaag ttcggttggt ggcggtattc gtacaacaac tttcgctatt ttaattttgt
     8101 ttttaattaa ctttagtaat aatgccgata aaacatccat taaagtttac aatagagaag
     8161 tacacattat ggatattcaa cgttcatttg cagtatttac aatggcgaca attttaacat
     8221 ttttaggaat gctaattata tcagctactg aaaatggtaa gcttacattt ttacaagtat
     8281 tttttgaagt catgtctgca tttggaactt gtggactatc gcttggtgtc acaagtgata
     8341 ttagtgatat ttctaaggtc gtactaatga tactcatgtt tataggacgt gttggcttaa
     8401 tatcatttat cattatgata gcaggacgtc gagaaccaga taaattccat tatccaaaag
     8461 aacgtattca aataggataa tataatagca atctaagttt agttaatgta gattttaact
     8521 ggaacttaga ttgctttttt agtttgtatt tttaacttat tttataagac gattggtttc
     8581 gaaaatggta aactagtaac aatgagaggt gtaacatgat ggaaaaaaat gaaaacatta
     8641 atgtagagat tttaactacg tcagatatgc atagtcattt cttaaatggt gattatggtt
     8701 caaatattta tagagctggt acttatgtta accaagtaag agcacaaaat catcgcgtca
     8761 ttttattaga tagtggcgga agtttagctg gctcgttagc ggcctattat tatgctattg
     8821 ttgcacctta taaacgacat ccaatgataa agttaatgaa cagaatgcat tatgatgcta
     8881 gcggtgtgag tccaagtgaa ttcaagtttg gtttatcatt tttaactcgt tcaattgctt
     8941 tggcacgttt tccatggtta tcagcaaata ttgaatacaa tgttactaag gagccttatt
     9001 tttcaactcc atattgtatt aaacattttg gtgacttaaa aattgctatc gtaggcgtca
     9061 cagcagatgg tttaatggaa aatgagtatt ctgaaatgga gcaagatgta tctattgaaa
     9121 agacattagt ggcatcaaaa cgttggatta gatatatcca tgaagttgaa gagccagatt
     9181 ttttgattgt aatttatcat ggtggattga ataaaattag taatagtacg aaaaataaaa
     9241 aggcaagttc gaatgaagct gaaaaattaa tggaagaact cggtgttata gatttaatga
     9301 ttacagctca tcagcatcaa acaatagtag gtcaagatca tgaaacgtat tatgttcagg
     9361 ctggtcaaga tgccaaagag cttgtacatc tttcgattaa ttttaaaaag agaacaacaa
     9421 cttatgatgt tgaaagcatt gattctaaag tgattgactt aaatgagtat gaagaggatc
     9481 aagaattatt agatttaaca ttctatgata gaaaagcagt ggcttattgg tcacaggaaa
     9541 tcataagtga taaaggtttg atgttatcag taaatgggtt acaagattta gtctgtcaaa
     9601 cacatccatt ttcgcaatta ttacatgatg caattcacct tgcatttgat aatgatataa
     9661 catgtgtcca cgtgcctatg aacggagaga aggggttgag tggacagatt cgaaatgaag
     9721 atttgtatca tgcataccca tatccagata agccaatgga tatgacaatt agtggtcaaa
     9781 atatcaaaga tatattggag tatagttatt cacatttaga ttttgttaac gagcaattaa
     9841 gcttaacaat tattgatgaa acgttatgta caatgtggca aggattcaat tatgagattg
     9901 atatgaatca agaacctggg caacgagtaa tgttagatca aattgatttg actaagagtt
     9961 atagagttac aatgactgac tattgttatc gtaactacaa gaattattta aaaaatgcta
    10021 ttatacatga atcatacgat gaaacaatga gtacattaat tgcagagaag ttaagagatc
    10081 cgaattatcg tatttcatgt agtgataatt ttatagttaa aaacaggtaa atattgctat
    10141 gtaataaatt aattataagt tcaacaagta taaaaacctt tcttagagaa gattatctag
    10201 gaaaggtttt tagaatttta aaattgatat aaaagaacag tgaaattctc attcattata
    10261 tataagtaaa caaaaaagct aaaatggtat ataccatttt agcaagtaac ggaaacggag
    10321 ggattcgaac cctcgcgccg ctttcgcgac ctacaccctt agcaggggcg cctcttcagc
    10381 caacttgagt acgtttccaa tggctccaca ggtaggactc gaacctacga ccgatcggtt
    10441 aacagccgat agctctacca ctgagctact gtggattaat ttgtatgtgt ttgaatatca
    10501 agcacaaata ttattataac aagattaagt taatttgcaa tagatacctt aaaaaaatat
    10561 gcagaacatt tacactatat ttataaaaca ttccgcatat tttaaaaata aacaatttat
    10621 ttatcttcaa ataaagaata gcgtgtcaat gcttcaaaca cattcaatgt ggctttatta
    10681 taatctattg gttggtcggg attttttgat atcatgcgag attgtttatc taccatgtaa
    10741 tagtaaaaaa tggaattgtt atattgatgc cataaactgt ggtatgaaac atgaagaatg
    10801 attgattcat tattaataaa tgtcacttta catttacgat tttttaattc tttaatactt
    10861 tcgatataat gcatatttaa ccaaatattt tcattttgtc tgtcagaatg tgttgggaaa
    10921 aaataagttg gaaataatgg tgttagtaaa ataggtggtt tactagaaat gccagtaatg
    10981 cggtttgtct ctgctttctt gccaagataa gtattaccat aaaatttgca agagcgttca
    11041 atgattttct ggactttaaa aggattttga atacgcgttt tgtcaaaccg aattatttca
    11101 ctaccgtttc tttgatcgtc atcatcaaat gctggtcgaa taaccatgtc tcctttgcgt
    11161 atcacataaa tattttgaga atacataagc aaaacctcgc tttattaagt tttaa
//
LOCUS       DAKTLH010000083        11163 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.083, whole genome shotgun sequence.
ACCESSION   DAKTLH010000083 DAKTLH010000000
VERSION     DAKTLH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.083"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..196
                     /locus_tag="O0394_001781"
     CDS             <1..196
                     /locus_tag="O0394_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCY0499854.1"
                     /translation="SERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSA
                     KDIKKIDIDVKQYTKSKKNK"
     gene            207..497
                     /locus_tag="O0394_001782"
     CDS             207..497
                     /locus_tag="O0394_001782"
                     /inference="COORDINATES: protein motif:HMM:NF015598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCY0499855.1"
                     /translation="MKFPVTINKFENIVSNEFVFYNASKITINDLSIKLKSAMANDQG
                     ITKHDIGLAERAVYKVYFKNGSSKYVDLKTEYKDERVFKATDIKKVDIELKF"
     gene            complement(821..2107)
                     /locus_tag="O0394_001783"
     CDS             complement(821..2107)
                     /locus_tag="O0394_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0499856.1"
                     /translation="MNPLAQSLNEQLQQSNATAFTMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            2307..2405
                     /locus_tag="O0394_001784"
     CDS             2307..2405
                     /locus_tag="O0394_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499857.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
     gene            2648..2824
                     /locus_tag="O0394_001785"
     CDS             2648..2824
                     /locus_tag="O0394_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499858.1"
                     /translation="MKHLTKIFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNSK"
     gene            3088..3468
                     /locus_tag="O0394_001786"
     CDS             3088..3468
                     /locus_tag="O0394_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0499859.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            3465..4361
                     /gene="pmtA"
                     /locus_tag="O0394_001787"
     CDS             3465..4361
                     /gene="pmtA"
                     /locus_tag="O0394_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HCY0499860.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYSENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIYEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            4362..5042
                     /gene="pmtB"
                     /locus_tag="O0394_001788"
     CDS             4362..5042
                     /gene="pmtB"
                     /locus_tag="O0394_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HCY0499861.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFVLSQSSFPDLYSYILNIGFLIISIVILIVNYFKQLNKINT
                     RKFKGGSR"
     gene            5039..5911
                     /gene="pmtC"
                     /locus_tag="O0394_001789"
     CDS             5039..5911
                     /gene="pmtC"
                     /locus_tag="O0394_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HCY0499862.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
     gene            5911..6651
                     /gene="pmtD"
                     /locus_tag="O0394_001790"
     CDS             5911..6651
                     /gene="pmtD"
                     /locus_tag="O0394_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HCY0499863.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVAKKDI"
     gene            complement(6717..7280)
                     /locus_tag="O0394_001791"
     CDS             complement(6717..7280)
                     /locus_tag="O0394_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0499864.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
     gene            7401..7925
                     /locus_tag="O0394_001792"
     CDS             7401..7925
                     /locus_tag="O0394_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499865.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            7985..8542
                     /locus_tag="O0394_001793"
     CDS             7985..8542
                     /locus_tag="O0394_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCY0499866.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEG
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            8539..9381
                     /locus_tag="O0394_001794"
     CDS             8539..9381
                     /locus_tag="O0394_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCY0499867.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNLSSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
     gene            complement(9438..10478)
                     /locus_tag="O0394_001795"
     CDS             complement(9438..10478)
                     /locus_tag="O0394_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCY0499868.1"
                     /translation="MQQVTSDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYQPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            10929..11102
                     /locus_tag="O0394_001796"
     CDS             10929..11102
                     /locus_tag="O0394_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499869.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
BASE COUNT         3879 a         1437 c         1879 g         3968 t
ORIGIN      
        1 aagtgaaaga ggcatcagtg atcttgactt aaaatttgcg aaacaagcaa aatatacagt
       61 atatttcaaa aatggaaaga aacaagtagt gaatttaaaa tcagacatct ttacacctaa
      121 tttatttagt gccaaagata ttaaaaagat tgatattgat gtaaaacaat acactaaatc
      181 aaaaaaaaat aaataaatct aataatgtga aattcccagt aacaataaat aaatttgaaa
      241 acatagtttc aaatgaattt gtgttctata atgcaagcaa aattacaatt aatgatttaa
      301 gtataaaact taaatcagca atggcaaatg atcaagggat aactaaacat gacataggac
      361 ttgctgaacg cgcagtgtat aaagtgtatt ttaaaaatgg ttcgtcaaaa tatgtagact
      421 taaaaactga gtataaagat gaaagagtat ttaaagcaac tgacattaaa aaggtagata
      481 ttgaacttaa attctaatct ttaattttat aataaggcat ctcacaatcg tggggtgcct
      541 tttacatttg tagagatgtg atgcttgaat gtttgaagtg atttgccaaa agttatataa
      601 attggttcta taataaatcg gactgataga aaaagttttt tacttttcat ctgtccgatt
      661 ttttgatttt gttaggagag atgtataaaa ctaattaggt atagtgtttg acaagttgct
      721 gaataaaata tatcttatga tactttgaaa gaaggaataa ttttaaaaat aaaaaaccaa
      781 taatccgagt catactcatc agattattgg ttgaaattaa ttatcttaag tcatcaatcg
      841 ctttagcaat tgaatcaaat acatgtggga tatcttcttt ttctacacag ctaaacgcaa
      901 tacgaatatc tgttgcatta agtgcaataa caccaattga atatttttca attaaatgtt
      961 tacgaagttc ttcagggtca acatcatgaa cttttattgc cataaagtat ccagagttaa
     1021 agtcataggc ttgccaatgt gaatgatatt gatcagcata gacaacttcc ttagtgactt
     1081 catagcgttc ttttaatgtt tgaatatttt gttcgatttc tttatcgaat tgtttattat
     1141 tttttaatac atgcttaaca gcgctttgtg ttggaagagg tccactagaa atgttacttc
     1201 gtataagacc ttttactttg gcttctaata cttctttagt tgtttgatca gacgttccaa
     1261 atgtcataaa cccaacacgg aatccccatg cgaaaaattc ttttgttgca ccatctaaac
     1321 gaacaggtaa tattgcattt gaatttaaat tagataatgc agtaaataat gattgagtat
     1381 acacatcttc atagaataaa ccatagtatg cgtcatccac aacagctata acttttgtac
     1441 ctttatcagc taatgcttta attgcatcga cgatggtagt cacttcttta tgcgtaggtg
     1501 tgtaacctgt cggattatta ggataattta aaatcataat gactttatct ttattgtatg
     1561 attgtaaagc ttctacaagt gaatcagtag tataatgccc gtctttatca aagataggat
     1621 atgtttgaag gtttgcacca tttctcgtat tgaaaacaag tttgtaatta ccccaattat
     1681 gctctggtaa caagatagtg tcaccttgat ttacaaataa atcgccaact aaagataaac
     1741 catgtgttaa tgcattcgta acaattggta gtgacatgtt gtcgattgat agctctggat
     1801 tgtcacgcaa cattttttgt tgccataaat cacgtaattc ttcgatgcct tgtggtggcg
     1861 cataagggaa tatttcatct ggagttaaat cattaaacat tgcatctaaa gatgatgcaa
     1921 acatttttcc gtctttgttt gtcgccatac ctatagttgc attatatgtt gtactctttg
     1981 cttcagcaga ttgagataaa atgccttttg gataaaacat attttgacct aagtcagata
     2041 acattgtaaa ggcagttgca tttgactgtt gaagttgttc atttaagctt tgggctaaag
     2101 gattcattat tgcttaacct ctctttaaaa aaattgtatg cctatatatt atcttcaatt
     2161 ataccaaaga taaagtgttg ttggtgaatt aacgaataat atgtaaaatc tgctgccaaa
     2221 atacgaacat atgttctgtt tttatgttag aataggtgta ggtcatcctt cagtatgata
     2281 ctttagtcga acggaggtgt agtgtaatga tgtggtggca agaagggatg atgacactta
     2341 ttacaggtgg cttactaatt atttttcgac tttggttaga actgaaatgg aaaaataaaa
     2401 aatgatccta cacacataaa taaccccaag tctgctggta ccagacttgg ggttgtgtag
     2461 tataattctg tggatacact taaataatat cattgaacac aatatatcgc aatcaataat
     2521 cattttgatt gaatggtttt gttgcgctat tttagttgaa agtgattagt accttgaact
     2581 tttgaaattc aataacttat taaaaaaatt tatatttgtt ataatgtcga tgttgaggtg
     2641 aaataaattg aaacatctaa caaaaatatt tgtagtactt gcaattattt tatttattat
     2701 tggttattat ttacaagcaa ctaatcatga aagccaaggt ataaaattat tattagcagc
     2761 gattatgttt atgatttgtg cttttataag tagaagtaat gatcgacgta aaaatagtaa
     2821 atagaattga ctgtttaaaa cgattgttta tgttgacgct acggttgtca cgtagagatt
     2881 aaagacattc gttttagcag tcttttttat tgaagtagac aatgcaagat taaatggtag
     2941 tgtcatttca ttttatttta ttcaactgct agtaatacaa tactcagtgt tatttctgaa
     3001 gactggttga taatgagaca tgcatattta caaaactgtg tatattgtgt atattgtata
     3061 tatacagaag ttgatagggg gacgttgatg aaaataattt taaaaaataa tagtgatttt
     3121 ccgatttatg aacagattaa gcaacaagta aaacaaaata ttttaaaggg acatgttgct
     3181 cctggagagc atttgccgtc aatgagagaa cttgccaaag atcttcaagt aagtttgatt
     3241 actaccaaac gtgcttatga agatttagag aaagacggtt ttgttacaac aattagagga
     3301 aaagggacct ttgttaagga gcaagatagt tctattttaa aagagaaaca attttttacc
     3361 attgaaaatt tggttaaaga attggttaat gaagcgcaag ccatcgaaat gtcacttgag
     3421 gaactgcagg atattttaac gttcatttat gaggaggaat catcatgaat gccatagaat
     3481 taagtaatgt taattattca agtgatcagt ttaatttaaa gaatatctct ttcaaagttc
     3541 ctcaaggatt cgtcactggt tttattggta gaaatggcgc tggtaaaacc acaataataa
     3601 ggttaattat ggatttgtat caaccacaaa ctggtgttat tagagtatta gaagaggata
     3661 tggctctcaa tccgatagag ttaaagaata gaattgggtt tgtttactct gaaaattatt
     3721 ttaatgagag atggactacg aagcaacttg aaaaaatgat tgcccctttt tatcgtaaat
     3781 gggaccatca agtatttgaa ttttatcttg aaaagttcga tttaccaatc aataaatcaa
     3841 taaaaacatt ttcgactggg atgaaaatga aattatcact agcagttgca ttttcacatc
     3901 atgctgaatt gtatattttt gatgagccta cttcaggtct tgatccctta gctagaaatg
     3961 agctattgga gataattcag caagaattaa ttgatgaaaa taaaacaata ttcatgtcga
     4021 cacacattat ctcagattta gaaaaaatag ctgattatat cattcactta agtgacggtg
     4081 aagttatctt aaatggttca aaagaacaac tgttacaaag atatcaagtt gtgagtggtg
     4141 ctattgagga ccttgatgat gaattagcat ctctacttat ttatgaagaa cataagcgta
     4201 cgggatttat aggattaaca gaacatgcgc aagtatttaa agaaatttta ggtcataaag
     4261 tgaatatcac tacaccttct attgagaact taatggttta tcttgaaaag agaaaaccta
     4321 aatatcatga aaacatcaaa ttgatggaag aaggttttta aatgaaggca ttgttaatta
     4381 ggaattttaa attacgacga tatacactta tcatctatgt tctattatta accttatatc
     4441 ctttttatat aatgcttgac tcaacaaagt tcttttactt attgcaatct tttataagtc
     4501 caacaatatt gattatttgg attttagatg ctggtcactt atttagactc aatcgtagat
     4561 taggtggtaa tgattcatat tatttttata tgagtttacc agtgtcaaaa aaacaattgc
     4621 ttaatgccaa ctatattaca tgcattgttt taacattaat tggaacgctt gttattagtt
     4681 tatatgctta tgaagcagat gtgattgaac caaattcaat ctatttttca actgcatatg
     4741 catttgtcat atctaacttc ttgtctatac caattgcatt tagtcaattt acagaattgc
     4801 gtagagtcaa agtgccatat ggtatatacg tgtttactat tatcatttta gttccatttt
     4861 tattttcaat tgcaatagta ttggtgaatt attttgttct aagtcaatca tcattcccag
     4921 atttatattc atatatttta aatattggtt ttctaattat aagcattgtt atacttattg
     4981 ttaattattt taaacagctc aataaaataa atactagaaa atttaaagga ggcagtcgat
     5041 gaaattagaa catattacaa aaaaatacgg ctcaaatgtc gttttaaatg atattgattt
     5101 tgactttggc gatagtagaa ttgtcggatt aataggaaaa aacggtgttg gtaaaacaac
     5161 cgttatgaaa gtaatgaatg gtaatattat taaatttgat ggaaaagtag atattgataa
     5221 tgcagataat atcggttttt taattgagca tcctaaatta tatgataata aatcaggatt
     5281 gtataacttg aaattatttg cacaagtatt aggtaagggt tttgataaag catacacaga
     5341 caaaattata gatgcatttg gtatgagacc ttatattaaa aagaaagtta agaaatattc
     5401 aatggggatg aagcaaaagt tagcaattgc agtatcttta atgaataaac ctaaattttt
     5461 aatcttggat gagcctacaa atggtatgga tccagatggc tcaattgatg tgctgactac
     5521 aattaagtct ttagtaaatg aacttgatat gagaattcta atatcaagtc ataagttaga
     5581 agatattgaa ttaatttgtg atagagctgt atttttaaga gacggacatt ttgttcaaga
     5641 tgtaaacatg gaggaaggtg ttgcatctga cacaacgata gttactgttg atcataaaga
     5701 ctttgataga actgaaaaat atcttgcaga gcatttccaa ttacaaaatg tcgacaaagc
     5761 agacggacat ttaatgatca atgcacaaaa aaattatcaa gttatactaa aagcattatc
     5821 tgaattagat atttatccga aatatattga aacacgtaaa agttcattgc gtgatacgta
     5881 cttcaatata aatcaaagag gtgataaata atgagaattt taaatttagt taagtatgat
     5941 ttttatagta tatttaaaag tcctttaaca tatttagcga tactagtcgt atctagtttg
     6001 attgcaactc aaagtatact tatggcaaat tcgatggata acccgaaaca tattattgtc
     6061 tatggatctg tatttgctgc agcaaaatgg ttattgttaa taattggatt aatgtttgtt
     6121 gttaagacaa ttacgcgtga tttttcacaa ggtacaattc aactatatat gagtaaagtt
     6181 aaaacacgcg ttggatacat tatttcgaaa acaatttcaa ttattttaat ttcaatatta
     6241 tttgcattaa ttcattatgt gattttgatt gttgtgcagg catctagtaa tggaaaaaat
     6301 ttggcgtttt ctaaatatgt agataattta tggttcttcc taatcttttt actattcttt
     6361 ggcttgtttt tattcttaat cacacttgca tcacaaaaaa cagcaatgat attttcatta
     6421 ggtgtatttt tagtactcat tgtaccgttt attaaacctt ttattacatt tatcccaaga
     6481 tacggtgaaa aagttttaga tgcttttgat tatatccctt ttgcttactt aactgataaa
     6541 atgattagct ctaactttga ttttagcaat tggcaatggg taatttcatt aggttctata
     6601 gtgatattct tcattttgaa tatcttatat gtcgctaaaa aagacattta ataaaaataa
     6661 ttttgaggtt gggaatttta aattttccca acctcaaagt ttgtcttatt gtaaatttat
     6721 ttattttcta atttatttag gatggaatta taaactgctt tccaaaatga agcgtcagtt
     6781 ttatagcggt ttgatataac taagtgtgtt tctttttcta aatctgcata gtctggatga
     6841 tctttgctcg gtaatttatc agcacgaaca tcagttacaa atttttggac ttcatttgct
     6901 cttggtcccc aaactgtttc ttgttcgaat tgatcattca agaatacgaa gataggaatt
     6961 gcacgtgatt taccatttgt taaatattga tcgatcagtt ttgtatcatc atctctatgg
     7021 aacacgcgta cttctaaatt taatgcttca ctgatgtgtt ttagaattgg gagattcatc
     7081 attgcatctc cacaccagtc ttcagtaatt actaatactt tagaataatt catctctttt
     7141 atttttttga tgcgtgaatc atcttctggt aactcaaatg attgatagat actgagaacg
     7201 gtatcttgat ttgtcttcat tccatcaatg tattcattta agggttggct atttttgaaa
     7261 taagtttcta aatttgtcat tgtaaaaacc tcctttagca tttacaacat tataccaatt
     7321 tatagtaata aaaggtaatg aaataaatta attgcaaatt ctttgttaat ttttgttaag
     7381 gatgaaacgg gaagcacctt atgctatatt taaataagta caaagaaagg gtgacatcag
     7441 tgcgtattca aaatcgctgg gttgtgttta tattattttt aatctgttct tttggtgtat
     7501 taattggttt atatcaatat cgtcatacga aaactgtgga tttgtctaat cttgaaataa
     7561 acgatattaa attaaatgag aagtttgata aaaaaggcta tgaagtaaat aaaaaaatta
     7621 agtttgatcg ttttaaattt tataacagta aagctcaccc tgatcttacc gttaaagtga
     7681 gagaaaagga taacatcgtt aaggggataa tattagtaag agatgaaaag atacatacta
     7741 attttgatgg gggaattggt tcgccgataa ataacgcgat tgaaaatctt ggattcggat
     7801 ataaaagaac aaaagttggc aatgatttct catcggtaaa gtatattgat agagataacc
     7861 atttaaaatt aaacttactt tatcaagatt tagaaattaa acgtattgaa ttttttagta
     7921 aatagcttta ggtcttaaag ttataaaaaa cgaatgaata attttattgg gatgagtgac
     7981 ctatatgaac aagattactt atttaaatga attagaaaca gcacttgatg cgctacctag
     8041 gcatttaaga gatcaaaaaa tgtatgaata tgaacgttat ttttatgagc aagaacttaa
     8101 tggcgttgat gaaggggaaa ttttaaagaa gttaaaagac ccacaagatg ttgcagctga
     8161 aacaaaagct agaagtgtca ttgattatgc tgaatctaaa ccaacatttg aaaatatttc
     8221 aagagctgtt gctgcttcat taagtttagg cattctatct atttttgtca tccttatacc
     8281 agtatctata gttggattat ttgtattagc attattttta atatcacttt tgctgctgtt
     8341 ttgtccaatt attttattag catcagcaat atccagagga attgtggact caattagtaa
     8401 tgtatttttt gccatatcat attcaggatt aggattagta tttatcattg tcatatttaa
     8461 gattttagaa tacatttatc gtttaatctt aaaatattta ctttggtata ttaaaactgt
     8521 caaaggaagc gttagaaaat gaagaaattc ttttttattg ggcttttagt gtttgttgtc
     8581 ttttttacag cagcaaccat tatttggttc agctatgata aaaacaaata tggtactaaa
     8641 caatatgata aaacattcaa agacgatgct tttgacaatg tatctataaa tttggatagt
     8701 acagaacttc gtataaaacg ggggaatcaa tttagagtta aatatgatgg tgacaatgat
     8761 atattaatta atatagtaga taagacgttg aagattagtg ataaaaggtc taagacaaga
     8821 ggatatgcaa ttgatatgaa tccttttcat gagaataaga aaacgttaac gattgaaatg
     8881 cctgataaaa tgattaaacg tttaaatcta tcatctggag caggaagtgt tagaatcagt
     8941 gatgttgatt tagagaacac aagtattcaa agcattaacg gtgaagtagt tattaaaaac
     9001 tcaaatcttg acgcactaga ttcaaagaca aataatagtt ctacttatat tagtaaaagt
     9061 aacattaaaa atagcaatat taaagttgtt attggtacgc tacaaatcga caagagtcaa
     9121 attaaacaat ccatattttt aaacgatcat ggtgacattg aatttaaaaa catgccatca
     9181 aaagtagatg caaaagcttc tactaaacaa ggagatattc gttttaagta tgatagtaaa
     9241 cctgaagaca ctatactaaa gctaaatccg ggaacgggtg atagcgtagt taaaaataaa
     9301 acatttacta atggtaaagt tgggaaaagc gacaatgttt tagaatttta tacgattgat
     9361 ggtaatatca aagttgaata aataaaggat gtaagcaccg atattaggaa gcataatttc
     9421 tctaatatcg gtgttattta tttgttggca aaagttaagt cggtatctat attgccagta
     9481 aagtgagtga tattaaggtc ttgaccatct aaccatgatt tgaaatctat tatttctggt
     9541 ggcgcatttt ctcccaatgt aaaatatgca gttaatgttt caggttgata cattgatgta
     9601 tggatggtgc cagaccagct tttgaatagt ttactgtaaa tttcatactg aggattattg
     9661 aataacttaa atgctgtagt catatctaaa ttatcattag tttgtgaaat ggtacgcgcc
     9721 agtctttctt tagattcttt tgtataatta cgattttcat gtgttaatat ttcaaaatga
     9781 tttgtacata tattatcata acgaacatct attgatctcg gtgtcacttc aacaattgca
     9841 tggttcaatg atttgtccat cagtatgtag ctaaatgagc ttctgtgtgg tatttctttc
     9901 aataattgga ttgcttctgt tacatttcgg caattttcaa gaattagacg accaatcata
     9961 taacatacaa aaccatttgc tggtttcttc cggtgcataa agttatagcc catagttaat
    10021 cctgactcat tcataccatc cattcttcca gttacccttg atacaggacc aatttgagct
    10081 aaaccgctat ctgtaggttg ataaagtaag tagcgaccat cataagttgc agggtggtaa
    10141 tcataatttc taaccatgaa gtctttgcct tgaaagaccg tgcaaccact ttcttttaaa
    10201 tcggtaaaac gataatgtcc aaagtttaaa ataatttggc gtgttggcat tttgagtata
    10261 ctttgtagtc ccattaattc ttcccatatt tgaggtgcgt atgtttggaa tatttgataa
    10321 gtttcattta catctatatc gaaacgtggg acacgttttt tccattcttt ttctcgattt
    10381 tttagaagag gtgtttgttg aagccattta ccagttttaa cacctaactc gaaatgtgaa
    10441 cctctaaaag tcatgatatc tgatgtcact tgttgcatat catcggcccc tttcttttta
    10501 gttgtaatat attgtaaata aatagtaatc gtatgtatat tgaatgtcat gttaaataaa
    10561 gttatatttt actaaatgaa atataaaatt gtttgaggtg atttctcggt gtataagact
    10621 tatcaatcag ttaaaacata tttttataga tggtggggat attgagttaa aaacttaaaa
    10681 tcatcttatc ataaatatca atcttaagtt agcattcacg ataatagtca ttgttaacat
    10741 tagcatataa ggtcatgtca cgttgaaaca gaggttcctc ggcatttttg aaaataatca
    10801 ttagcttgct cactatataa tttgatgaat atatttcgtg aaagtgggta tttatttaat
    10861 gattattcta tatatgatag tataatgaaa tgtagatagg tatttaattt aacagaggtg
    10921 aaattgagat gtggaatttt attaaatgtg tgtttaaatt cgtatttagc ttagttgcta
    10981 ttacaacatt agttgctggt gttggtgtag tagcatttgc ttatatcttt aaaaaagatt
    11041 ttgaagatat tgaaagaaaa actaaagaaa ttatttctga tattgaaagt aaaaataact
    11101 aataacattt agaggctggg acataaatcc ctaaaaaaca gcagtaagat aattttcaat
    11161 tag
//
LOCUS       DAKTLH010000084        11041 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.084, whole genome shotgun sequence.
ACCESSION   DAKTLH010000084 DAKTLH010000000
VERSION     DAKTLH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11041
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.084"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            172..1242
                     /locus_tag="O0394_001797"
     CDS             172..1242
                     /locus_tag="O0394_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCY0499870.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            1438..2199
                     /locus_tag="O0394_001798"
     CDS             1438..2199
                     /locus_tag="O0394_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCY0499871.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            2217..2711
                     /locus_tag="O0394_001799"
     CDS             2217..2711
                     /locus_tag="O0394_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCY0499872.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            2725..3951
                     /gene="pepT"
                     /locus_tag="O0394_001800"
     CDS             2725..3951
                     /gene="pepT"
                     /locus_tag="O0394_001800"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCY0499873.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDVDVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(4140..5264)
                     /locus_tag="O0394_001801"
     CDS             complement(4140..5264)
                     /locus_tag="O0394_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCY0499874.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAYQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(5689..6009)
                     /gene="ytxJ"
                     /locus_tag="O0394_001802"
     CDS             complement(5689..6009)
                     /gene="ytxJ"
                     /locus_tag="O0394_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HCY0499875.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            complement(6163..7041)
                     /locus_tag="O0394_001803"
     CDS             complement(6163..7041)
                     /locus_tag="O0394_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HCY0499876.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            7161..7679
                     /locus_tag="O0394_001804"
     CDS             7161..7679
                     /locus_tag="O0394_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HCY0499877.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYTKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFNTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
     gene            7806..8729
                     /locus_tag="O0394_001805"
     CDS             7806..8729
                     /locus_tag="O0394_001805"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCY0499878.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
     gene            8747..9061
                     /locus_tag="O0394_001806"
     CDS             8747..9061
                     /locus_tag="O0394_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499879.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(9378..10406)
                     /locus_tag="O0394_001807"
     CDS             complement(9378..10406)
                     /locus_tag="O0394_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0499880.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(10525..>11041)
                     /locus_tag="O0394_001808"
     CDS             complement(10525..>11041)
                     /locus_tag="O0394_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0499881.1"
                     /translation="TNHTEMNITVEQLVNFGRFPYSKGRLTKEDHDIVNDALDLLQLQ
                     DIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLDEPLNNLDMKHAVQIMQTLKMLAHK
                     MNKAIVIVLHDINFASCYSDQIVALKNGQLVKSDLKDNVIQSSVLSDLYDMNIQIEHI
                     RNQRICLYFKD"
BASE COUNT         3577 a         1894 c         1613 g         3957 t
ORIGIN      
        1 tacgattcat ttcttatata tcgcctatta ttcatttttt catcatatca tttaaggcaa
       61 cattttaaca tcaaagtcta tatcatacat taattttata tgtaaactaa tgacaaattg
      121 taagttttca cttacaatag ctagtggtca attaaaggag tcgattcaga catgttcgaa
      181 gcatttatat acaatatatc tgttatagtc gctggaatct atttattcca tcgcttacag
      241 tattcagaaa acaaacgtat ggttttttct aaggcttatg tcactgtttt aatgacaatc
      301 gtatcattat tattatctgt ctaccctatc ccttatcgtg aagattattt aattcactta
      361 acattcgttc ctttattgtt tttaggtaga tttacaaata tggtctatac attatctgcc
      421 acagtaattg tagcaattgt tgaaattgta gtatttaaca actcgattat gtacggtgtt
      481 acgttgattg ttattgctgc agtaacaagt gctattggac catttttaaa acaaaatgat
      541 gtgctatcat tattaatact taatgttgtt acaataatca ttttatttgg tgtcgcgtta
      601 gtgagtccta tttacacact atcggaagtt atcattttaa taccaatttc attaattatc
      661 actttagctt cagcgataac atttgtcgat atttggcatt tcttctcact cgtaaatcgt
      721 tatgaaaacg aagataaata tgattactta acaggcttag gtaatgtaaa agaatttgat
      781 agacatttaa atgaaatttc acgaaaagct gaaaaagaac atcaaagtat cgcattatta
      841 ttaatcgata tcgatggatt taaagatgtc aatgatacct actcacacaa atcaggtgat
      901 gctgtattaa aacaaatgtc tcaattactt aaaaactatg tgccgaatca atttaaaatt
      961 tttagaaatg gtggcgaaga gttctcagtt gtcattcaca attattcact tgatcaaagt
     1021 gttaaattag ctgaaaatat tcgttcgggt gttgaaaaat catcattcca tttaccaaac
     1081 aaagaagtta ttaaattgtc agtgtctatc ggtgttggtt atttaacaga tgacgatcca
     1141 aaatcacagc gtaaagtgtt taaagatgca gacgatatgg tgcatgtagc caaaaatcaa
     1201 gggcgaaaca aagtaatgtt taaccctatt atcaatttat aacaacgaca aaactgttaa
     1261 tcctttgcta ttattaaatc atagtataat agcaaagatt aacagttttt cttatttaaa
     1321 tttgaaccat acaagttcat catactaaca caaagtctca ctattgaaag ttgtaacaac
     1381 aagttgtaaa cttcaattgt tcaaataaat aacacatatc attggaggtg aattgatatg
     1441 ccagattcaa tcacaattat agatgaaaac aaagtgattg atgttgtatt aattgcaggt
     1501 agaattttac ttgaatcagg tgctgaaaca tatcgagttg aagatacaat gaaccgtatc
     1561 gcacatagtt atggtcttca taatacatat agttttgtca gttcaactgc aattattttt
     1621 tcattaaacg atcgaacaag tacaagatta attcgtgtac aagagcgtac aacagattta
     1681 gagaaaatcg ctttaacgaa tagtctttca cgtaaaatat caaataaaga actcacaatt
     1741 gatgaagcta aatctgaatt tattcattta cagcatgcat cattacaata ttctttttta
     1801 acgaatttct ttgcagctgc cattgcatgt ggcttcttcc tatttatgtt tggtggtgtc
     1861 gcatcagatt gttggattgc agtcattgct ggcggatccg catttttaac attcagcttt
     1921 gtgcaacgct atatacaaat taaatttttc tcagagtttg ttgcagccgc tgtggtcata
     1981 tccattgcag ccacatttac taaattaggc attgcaacca atcaagatat cattactata
     2041 gccagtgtca tgccacttgt ccctggtata ttaattacca atgccattcg agatttatta
     2101 gcgggtgaat tacttgcagg tatgtctcgc ggtgttgaag ctgcattaac agcattcgca
     2161 atcggtgctg gtgtcgcaat cgttttatta atcatttaag gaaggactgt taaagcatgc
     2221 tattttattt atttcatttt acaatcagct ttatatcaac agtacttttc tctatcattt
     2281 tcaatgcacc caaacgcctc ttagtagcat gtggatttgt gggtgccatt gcatggacga
     2341 tttaccaatt aacggtagat ttagagtttg gaaaagttgg cgcttcattt ttgggaagct
     2401 taattttagg cttaatgagt catactatga gtcgcagata taaacgaccg gtaattatat
     2461 tcatagtgcc aggcattata ccattagtac ctggtggtgc agcttatcaa gcgactcgtt
     2521 ttttagtatc aaatgattat acaagtgctg taaatacatt tttagaagtt acactgattt
     2581 caggtgcgat tgctttcggt atattagttt ctgaaattct atattaccta tacacacgta
     2641 tcaaacaact gtatggtaaa atcaaaggta agacatataa aaaatcttac aacatgaata
     2701 atagagttta aggggtcgaa tataatgaag aaccaattga tagatagatt aacaagatat
     2761 acgacaattg atacgcaatc tgatccaaaa tctacaacaa caccttcaac tgaaaagcaa
     2821 tgggatttgt tacatttatt agaaaaagaa ttacaacaat taggattgcc aactgattta
     2881 gatgaaaatg gctacttatt tgctacatta gaaagtaaca tcgatgtcga cgtcccaaca
     2941 gttggatttt tggcgcatgt tgatacatca cctgatttca atgcttcaaa tgttaaaccg
     3001 caaattattg aaaactatga tggtaagcca tacaaactag gcaatactaa acgtgtctta
     3061 gatcctaaag tatttccaga acttaacagt ttagttggtc atacattaat ggtaactgat
     3121 ggtacatcgt tactaggtgc agacgataaa gcgggtattg tggaaattat ggaagctatt
     3181 tgttatttac aagaacatcc agaaattaaa catggtacca ttcgcattgg atttacacca
     3241 gacgaagaaa tcggtcgtgg tccacataaa tttgatgttg accgcttcaa tgctgatttt
     3301 gcttatacta tggatggtag tcaatatgga gaattacaat atgaaagctt taacgctgct
     3361 gaagcggtta ttacatgcca cggtgtaaat gttcatcctg gttcagctaa aaatgcaatg
     3421 gtaaacgcaa tacgtttagg tgaacaattc gatagtttgc tacctgatag tgaagttccg
     3481 gagcgaacag aaggatacga aggcttttat cacttaatga actttgaagg aactgttgaa
     3541 aaagcaactt tgcaatacat tattcgtgat catgataaaa aacaattcga attgcgtaag
     3601 aaacgtattt tagaaatacg tgacgatatc aatgcccatt ttgaaaatta tccagttaaa
     3661 gttgatatat cggatcaata tttcaatatg gcagaaaaaa tattaccatt gcctcatatt
     3721 attgatatac ctaaacgtgt ctttgccaaa ttagatattc cagcaaacac tgaacctatt
     3781 cgcggtggta cagatggttc acaattgtca tttatggggt taccaactcc aaacatcttt
     3841 acaggttgcg gtaacttcca cggcccatat gaatatgcat ccattgatgt tatggaaaaa
     3901 gcagtacaag taatcattgg aattgtagaa gatatcgctg aaaatcacta aaaaccaacg
     3961 attatatact agttcaactc aggcttaatt aatatataaa aatcacgcta acattcagtg
     4021 caatgatagc gtgatttttc atatttttta cgtatacttt cgatcttcta attcgttttg
     4081 ctaaccatca taaatgttat taaccatcat gacagcatca ccaatttagt ccttctttac
     4141 taaacctcaa cattaaaatg tgttttcaac agttttaaag actgaaccgt ataatgccta
     4201 atttgtaacc ccattttaaa gtcagtataa gtttctggca tatcgataaa tgtattaaac
     4261 attgctgtaa ccccttgtga ttcaaataaa tcaaactttt cagcagttgc acaaattgca
     4321 atggcaacct tttgatgttt atgacaaagt tctgcaatac gcaatgttgt cgtttctagc
     4381 aactgatcat tttcatttaa tccttctcca aaaataatta aatccgcttg ttcaactaaa
     4441 ttttctaaat gtgttagttg gtctactaat gcatgactgg ttaatatttc agcttgatac
     4501 agtccattca agactgctgc aattccacca ccagcaccac cacgttcaac tggaccaatt
     4561 gcaattttca attcactttt aaataactca ctaaaatacc aaattaaatt atcgatttct
     4621 gctgcttgat tatgattcaa ctgatacgca tcataagttt gcatgatttc actttgctta
     4681 ccatataatc gacttgaaaa atccgacatt acttgaattc ttgctgtttc cattttaggg
     4741 tgtaagttcg acatatccat acgacgaata tatttaatta caccagcacc ttgtctcata
     4801 tctacgacac gcccttcgtc atcatagaat tgagcaccta atgcttgtaa catacctgca
     4861 ccagcatcaa aactatcaat cccacctagt gaaattacaa catgttttgc gtcgttatct
     4921 aatgcatgtt taatcatttc tcctaaacca taacttgttc gttcaacaat tggtttttta
     4981 ccttttaaaa ataaattccc ctcgataacg gtcatccctg tatcagtttg tccgtaaaca
     5041 ccttcaactt cattcatatc tgcatcatgt actggtatac gatacttttg cccagattgc
     5101 cataaaaata cagaatctaa taattcatgt cttccattaa acaatggtac ttgaactaca
     5161 tctgcagttt caatttggct tgcaactgcc tcttcaacat atctattagc ttgataactt
     5221 gaaataattc catgaaactc atccatggct actaatactt tcataacctt gaccacctta
     5281 ctttattatt tgttgtgata cgttttttat tcataaaaga ttaatcttaa attttagatt
     5341 ttatctcgtg attatatgcg aacatttaac atgacatgat tattaattat tccagtaaat
     5401 aacaatatgc ttcttttggt ttagcatata tttcctatta aactaactta caaatcatgt
     5461 ttaaattttc ttattggatt cgtcgttttc caaaatattt tcaataaaaa tactcgaata
     5521 tattatacat atgtttgtaa aaaagtacta acttaactgc ttctaaaggt gacataaaat
     5581 aaaaggtcag aacatcatta tagccaaact actctgattc acacgaattt aagttgtgct
     5641 attcacgtct agtagtgtaa ggcaaaatgt tccaacccta tagtttcatt attcttctgc
     5701 ttgtgctaat gacgacacat tgatatcacc gtggtctcga ttccaaacca tttcaccatt
     5761 tacaaaataa aatgcttgag gtgattcatg tttaacgttc gtttttttag caatataatc
     5821 tgacaaatcg cgttcttgtt ggacaatcaa ataataaccg tccatatcgc gttcatataa
     5881 aaatttatta aattgatcgt acgcatttgc cgatattgga caagtttcac tatgttttaa
     5941 tacaaaaaca tatttatttt cctcaataac ctgttcaaat tggtcaattg aacttagctt
     6001 tatagccact ctattcacct ctaaaaatat ttatcacatt taaatagcat ctgcgtgaca
     6061 tttttataca tatagttaat atcaatgttt accattatac acatagtaag ttgacatgtt
     6121 aaccataaat tcaaaatttt taactttgaa aaatgattat agttattttt cttctaattt
     6181 ttcaagcttt ttttcaaagg ctttatcgcc gtgagttata tctataccct ttttcggcaa
     6241 cttatctaca tcgacttttg ataacttctc actaaccttt attgtttcaa tacgtgtatt
     6301 ataatagttc tgcagactgt aatacatttc tattgcgttt ttccttgcat tattaacatg
     6361 cttatcacta atattagttt ggttaatatc ggtaatttgc ttttcaatca ttggcataat
     6421 gtgattttta atcgcgcctt ttacttcatt ttctttacta tcatctaggt tcttcttcgc
     6481 agtatctctt aacgctttat tattatgttc taattttgtc gtaagattag ttgcttcact
     6541 tttattatca gctaatttaa tttctgattc aactttgtat ctattttttt caaattgttt
     6601 cgtataatct aaaatgtctt cgttatactt gatagattga ttacataaac ctatgaattt
     6661 ttttaattga tatatcgcat ccttcttctc atttgcaagc gtcatatatt ctttttttaa
     6721 tttcttaact ttcattgtat catcaggcaa attttttgct gacttataat atgcttcaaa
     6781 tttaggtatg agatggtttt taacatcttc ttgtagtgcc ttaaattctt tactattttt
     6841 atcagttgta tcagttttac ttagatgatc aatttctttc aaatgtatat tatccatgac
     6901 atgttgtaat tctttttgtt tttgctcaac atctttaaat tgcttagtaa attgatgttg
     6961 tgcctctttt tctttattac cacaagcact tattactaca aataaaatcg ctaaaacagc
     7021 gataataaca atttttttca tgattgcact ccttaacaga ttcattatac aaaaacatgt
     7081 ttattatgaa aatgaattat gttatagtat tccagacttt atcgtgatta aattaatttt
     7141 caatatcaaa ggagtttatc atgtattcaa atgttcaacc attcattaat agtgatttaa
     7201 ctaattgttt tcaatcacaa tacactgaaa ttgaatcatt attattcaac ctcttagaca
     7261 gcccagtaaa atatacaaaa catattggcg gtacgcatca ttttaaatat tcaacagagc
     7321 caattttaga tattttagta ggtgtagaaa atttgcatga tattacatcc ttagatgaaa
     7381 aacgcttaaa ttatgtaggg ttttatcgtt tgcatcataa atatactaag aaagttatga
     7441 tggcgaagtt taacaatttt catgaactaa aacaacaggt gcgtctacat atcattcaaa
     7501 tgaataccca aacatttgaa tcttatcttg cacttgatta cctcttatca actaatgatg
     7561 atattgcttt gcaattcaat acaaaaaaac accaaatatt gcaacatagc ttaacaatta
     7621 gacaatatga gatgcaaaag caacaatact ttgaaaaact atgtaaaaaa tacttataac
     7681 gacaataatg ccgtatttta taattatact aattctaata ctttcttttc aattttcgca
     7741 aatgaatttt aaaattggta taatactata tgatattaaa gacatgagaa aggatgtact
     7801 gagaagtgat aaataaagac atctatcaag ctttacaaca acttatccca aatgaaaaaa
     7861 ttaaagttga tgaaccttta aaacgataca cttatactaa aacaggtggt aatgccgact
     7921 tttacattac ccctactaaa aatgaagaag tacaagcagt tgttaaatat gcctatcaaa
     7981 atgagattcc tgttacatat ttaggaaatg gctcaaatat tattatccgt gaaggtggta
     8041 ttcgcggtat tgtaattagt ttattatcac tagatcatat cgaagtatct gatgatgcga
     8101 taatagccgg tagcggcgct gcaattattg atgtctcacg tgttgctcgt gattacgcac
     8161 ttactggcct tgaatttgca tgtggtattc caggttcaat tggtggtgca gtgtatatga
     8221 atgctggcgc ttatggtggc gaagttaaag attgtataga ctatgcgctt tgcgtaaacg
     8281 aacaaggctc gttaattaaa cttacaacaa aagaattaga gttagattat cgtaatagca
     8341 ttattcaaaa agaacactta gttgtattag aagctgcatt tactttagct cctggtaaaa
     8401 tgactgaaat acaagctaaa atggatgatt taacagaacg tagagaatct aaacaacctt
     8461 tagagtatcc ttcatgtggt agtgtattcc aaagaccgcc tggtcatttt gcaggtaaat
     8521 tgatacaaga ttctaatttg caaggtcacc gtattggcgg cgttgaagtt tcaaccaaac
     8581 acgctggttt tatggtaaat gtagacaatg gaactgctac agattatgaa aaccttattc
     8641 attatgtaca aaagaccgtc aaagaaaaat ttggcattga attaaatcgt gaagttcgca
     8701 ttattggtga acatccaaag gaatcgtaag ttaaggagct ttgtctatgc ctaaagttta
     8761 tggttcatta atcgatactg aaacacgctg tcgccattat tttaccgaag aagatattat
     8821 tgctattaaa tttaaatgtt gtaataaata ctatccatgc tataagtgcc ataatgagtt
     8881 tgaaaagcac gccattaagc gttggtctga gccttcattt aacgaaaaag caatcttatg
     8941 cggtgtatgc aaacacgaat taacaatcaa tgaatatatg atggtagagc gttgtccaaa
     9001 ttgccaatct cggtttaaca atcgctgtaa atatcactat catatttatt ttgaaattta
     9061 aacaacatag tcaaaacatt atagggagtg ggtcagacat gataaagcac cacttctgta
     9121 gaattccttt ttgaaattct ctatgtttgg gccccgccaa cttgcattgt cagtagaaat
     9181 tgaaaatcaa atttctctat gtttggagcc cactagtatt gaaatgaact tgttacaagt
     9241 acatgttcat tcaaccaacg actgacaaca gaacatttta gagtctagga cattgattga
     9301 cgtcccagat ttcctttttt aaagaaaaga ggggagcaat gattaagacc tttaaccatt
     9361 gttcccctct tttaaaatta ttttacaact ttatcaagtt cctcaatttg tttaattgta
     9421 gttgtagttg atccagctgc aaagtaccat aatttaggat ctaaattata tactttgtct
     9481 tctttaattg ctttaacatt ttttaataca ggattattta atgcttgttt cgcagttgat
     9541 ttaccactta tcgcttgacc tctatccatc gctaaaataa catctggatt ttctttatta
     9601 acatattcgt tagaaacatt ttgtccatga ttgctattac ttactttttt atcaactgca
     9661 ttgaatccta atgtatcgta aactaatcca ccaaaacgac ctttaggtcc aaatgttgat
     9721 aattcacctt cgttaactag taaatacata acagttttat tgaagttttt cgttttatct
     9781 ttcattgaag caattttgtt atctaaatct ttatttaatt ctttagcttt atcttcttta
     9841 tcgtaaattt ttccgatatt ttcagtgttt tgtttcattg aaccaattaa gttcttttca
     9901 tctgcaccaa cataaacaat tttcgcttta ggtgcagctt ttttgaattc atctaaattc
     9961 ttttgattag ctgtacgtcc agagataaag attacttcgg gtttcgtcgc agcaatttta
    10021 tcaaaattca cttcttttaa attaccaacg tttgtatatt tatcatcttt aaatgattct
    10081 aagaaattcg gtaatgactt accgccttcc cctttaggta atgcttttac tttatctgat
    10141 aagcccattt ctttcattac atctaatgcg ccatagtcta acacaactgc atttttagga
    10201 ttttttggta cttcaacagt ttctttaact tttttagcgt cactaccatc ttttttctcg
    10261 ccacgcattt tatagttatt ttcaattttt actgtatctt tagaaccttt agtttctgat
    10321 tttgattgtt ctttatcaga attgttaccg catgccgcta ataaaaacat tactgctaat
    10381 actaaatata agactgtttt cttcataata taatattctc ctcttttggc ttaatctgtg
    10441 tgcttgatgt gaaatataaa taaatgcttt acgtttaacc cttctttaat tggcgcatca
    10501 cccctttaaa gtgtctccaa attatcaatc cttaaaatat aaacaaatcc tttgatttct
    10561 tatatgttca atttgaatat tcatgtcata taaatcactt aaaacactac tttgaatgac
    10621 attatctttc aaatctgact taactagttg tccgtttttc aatgctacaa tctgatctga
    10681 ataacaggac gcaaagttaa tatcatgtaa cacaatgaca atcgctttat tcattttatg
    10741 cgctaacatt tttaacgttt gcataatttg aacagcatgc ttcatatcta aattatttaa
    10801 tggttcatct agcaaaatat attcagtatc ttgtgctatt gtcattgcaa tgtatgcacg
    10861 ctgacgttgt ccaccagata atgacttaat attacgattt ctgatatctt gtagttgcaa
    10921 caaatctagc gcatcattga caatatcatg atcctctttc gtcaaacgac ctttagaata
    10981 agggaatcgt ccaaagttta ccaactgctc taccgtaata ttcatttcag tatggtttgt
    11041 t
//
LOCUS       DAKTLH010000085        10923 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.085, whole genome shotgun sequence.
ACCESSION   DAKTLH010000085 DAKTLH010000000
VERSION     DAKTLH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10923
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.085"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            182..613
                     /gene="mraZ"
                     /locus_tag="O0394_001809"
     CDS             182..613
                     /gene="mraZ"
                     /locus_tag="O0394_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCY0499882.1"
                     /translation="MFMGEYDHQLDAKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            629..1564
                     /gene="rsmH"
                     /locus_tag="O0394_001810"
     CDS             629..1564
                     /gene="rsmH"
                     /locus_tag="O0394_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCY0499883.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            1578..1979
                     /gene="ftsL"
                     /locus_tag="O0394_001811"
     CDS             1578..1979
                     /gene="ftsL"
                     /locus_tag="O0394_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCY0499884.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            1960..4194
                     /locus_tag="O0394_001812"
     CDS             1960..4194
                     /locus_tag="O0394_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCY0499885.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            4486..5451
                     /locus_tag="O0394_001813"
     CDS             4486..5451
                     /locus_tag="O0394_001813"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCY0499886.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            5453..6802
                     /locus_tag="O0394_001814"
     CDS             5453..6802
                     /locus_tag="O0394_001814"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCY0499887.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            6818..8137
                     /locus_tag="O0394_001815"
     CDS             6818..8137
                     /locus_tag="O0394_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCY0499888.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEESNDKNNDSASSHANDNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNVISVDNEQPQSAPKEQNSDSIDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEENPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            8243..9655
                     /gene="ftsA"
                     /locus_tag="O0394_001816"
     CDS             8243..9655
                     /gene="ftsA"
                     /locus_tag="O0394_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCY0499889.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKDKEESKFKKLMKSLFE"
     gene            9688..10860
                     /gene="ftsZ"
                     /locus_tag="O0394_001817"
     CDS             9688..10860
                     /gene="ftsZ"
                     /locus_tag="O0394_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCY0499890.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
BASE COUNT         4050 a         1485 c         2149 g         3239 t
ORIGIN      
        1 tttcttacac ttttgatcat attattataa aaccttaata taccaagggt ttagcccgat
       61 ttatcttaat gataaatcgg gcattttttt gttttttaaa ataaatttca caaattttgt
      121 ataaatagtg gtggatagtg gggagatgtg gtaaattata tataaggtga ggtgataaaa
      181 aatgttcatg ggagaatacg atcatcaatt agatgcaaaa ggacgtatga ttataccgtc
      241 caagtttcgt tatgacttaa atgagcgttt tattatcaca agaggccttg ataaatgttt
      301 attcggttac actctagacg aatggcaaca gattgaagag aaaatgaaaa ccttacctat
      361 gacaaaaaaa gacgcacgta agtttatgcg tatgttcttc tctggtgctg ttgaagtaga
      421 acttgataag caagggcgta ttaacatccc tcaaaacttg aggaaatacg ctaatttaac
      481 taaagaatgt acagtaatcg gtgtttcaaa tcgtattgag atttgggata gagaaacttg
      541 gaatgatttc tatgaagaat ctgaagaaag tttcgaagat attgctgaag atttaataga
      601 ttttgatttt taaaatggag gaattgaagt gtttcatcat atcagcgtta tgttaaacga
      661 aaccattgat tatttaaatg taaaagaaaa tggtgtgtac attgactgta cgctaggtgg
      721 agcgggacat gccctttatt tactaaatca attaaatgac gacggaagat taatagcaat
      781 cgatcaagac caaactgcaa ttgataatgc taaagaggta ttaaaggatc atttgcataa
      841 ggtgactttt gttcatagca acttccgtga attaactcaa atattaaaag acttaaacat
      901 tgaaaaagta gatggaattt attacgactt gggtgtttca agcccacaac tcgacattcc
      961 agaacgagga ttcagttatc accatgacgc aacattagac atgcgtatgg accaaacaca
     1021 agaactaaca gcatatgaaa ttgttaacaa ttggtcatat gaagcgttag tgaagatttt
     1081 ttatcgctat ggcgaggaga aattttcaaa acagatagct cgaagaatcg aagcacatcg
     1141 cgaacaacaa ccaataacaa caacattaga attagttgac attataaaag aaggtattcc
     1201 tgcaaaagca agaagaaaag gcggacatcc tgcaaaacga gtatttcaag cactacgaat
     1261 tgcagtaaac gatgaattgt cagcttttga agattcaata gaacaagcga ttgaattagt
     1321 gaaagtagat ggcaggattt cggtaatcac tttccattct ttagaagatc gtttatgtaa
     1381 acaggtgttc caagaatatg aaaaaggtcc agaggtacca agaggattac cagttatacc
     1441 agaagcatat acacctaagt taaagcgtgt taatcgtaaa ccgattaccg ctacagaaga
     1501 agatttagat gacaataaca gagcacgaag cgcgaaatta cgtgtagctg aaatacttaa
     1561 ataaggagca atttataatg gctgtagaaa aagtgtacca accatatgac gaacaagttt
     1621 ataatagtat accgaagcaa caaccacaaa ctaagcccga aaagaagact gtttcgagaa
     1681 aagtggttgt acaattaact aaatttgaaa aagttttata cataactttg attactgtaa
     1741 ttgctatgtt aagtatttat atgctatctt taaaaatgga tgcgtatgat acgcgaggaa
     1801 agattgcaga tttagattat aaaatagata aacaatcaag tgaaaacagt gctttacaat
     1861 ctgaaatcaa aaagaattct tcttatgaac gcatatacga aaaggctaag aaacagggga
     1921 tgagccttga gaacgataat gtaaaggtag tgcgtagtaa tggcgaagca aaaaattaaa
     1981 attaaaaaaa ataaaatagg ggcagtccta cttgttggtt tattcggact gctctttttt
     2041 atattggttt taagaatttc atatatcatg attactggac attctaatgg tcaagattta
     2101 gtcatgaagg caaatgaaaa gtatttagtt aagaatgcac aacaaccaga acgaggaaag
     2161 atatatgatc gtaatggtaa agtgctagca gaagatgtag aaagatataa acttgttgca
     2221 gtaatagata aaaaggcgag tgccaattct aaaaaaccta ggcatgtagt tgataaaaaa
     2281 gagactgcaa agaaattatc tacagtcatt aatatgaagc cagaggaaat tgaaaagaga
     2341 cttagtcaaa agaaagcttt ccaaattgaa tttggacgca aaggaacaaa tttaacgtat
     2401 caggacaaat tgaaaataga gaaaatgaat ttgcctggta tttctttatt gcctgaaaca
     2461 gaacgctttt atccaaatgg caattttgca tcacacttaa ttggtagagc tcagaaaaat
     2521 ccggatactg gtgaacttaa aggtgcactt ggagttgaaa agatttttga tagttattta
     2581 agtggatcta aaggatcatt gagatatatt catgatattt ggggatatat cgcaccaaat
     2641 actaaaaaag agaagcagcc taaacgtggt gatgatgtcc atttaacaat cgattcaaat
     2701 attcaagtat ttgttgaaga agctttagat ggcatggttg aaagatacca gccgaaagat
     2761 ttatttgcgg ttgtcatgga tgccaaaact ggagaaattt tagcatacag tcagcgacca
     2821 acatttaatc ctgaaactgg taaagacttt ggtaaaaagt gggcaaatga cctttatcaa
     2881 aacacatacg agcctggatc aacatttaaa tcatatgggt tagcagctgc tattcaagaa
     2941 ggtgcttttg atcctgataa gaaatataaa tctggacata gagatattat gggttcacgt
     3001 atttcagact ggaatagagt cggttggggt gaaatcccaa tgtcactcgg atttacttat
     3061 tcatctaata cattgatgat gcatttacaa gatttagttg gtgcagacaa aatgaaatct
     3121 tggtatgaac gatttggatt tggaaaatca actaaaggta tgtttgatgg agaagcacct
     3181 ggtcaaattg gatggagtaa tgagttgcaa caaaaaacgt catcatttgg tcaatcgaca
     3241 acagtaacac ctgttcaaat gttacaagcg caatcagcgt tctttaatga tggtaatatg
     3301 ttaaaaccat ggtttgtgaa tagcgttgaa aatcctgtta gtaaaagaca attttataaa
     3361 gggcaaaaac aaatcgcagg caaaccaata acaaaagata ctgctgaaaa agttgaaaag
     3421 caattggatt tagttgtgaa tagtaagaag agtcacgctg caaactatcg tattgatggt
     3481 tatgaggtcg aaggtaagac tggtacagca caagtcgctg cacctaatgg tggtggatac
     3541 gttaaaggtc caaacccata ttttgtaagt tttatgggtg acgcgccgaa gaaaaatcct
     3601 aaagttattg tatacgctgg tatgagcttg gcacaaaaaa atgaccaaga agcttatgaa
     3661 ttaggtgtta gtaaagcgtt taaaccaata atggaaaata ctttgaaata tttaaatgta
     3721 ggtaaatcaa aagatgacac atctaatgca gagtatagta aagtgccaga tgttgaaggt
     3781 caagacaaac aaaaagctat tgataatgtg agtgcaaaat cattagaacc agttactatt
     3841 ggttctggca cacaaataaa agcacaatct ataaaagcag ggaataaagt cttacctcat
     3901 agtaaagtac tgttattaac agatggagac ttaactatgc ctgacatgtc aggatggacg
     3961 aaagaagatg tcattgcttt tgaaaaccta acaaatatta aagtaaattt aaaaggtagc
     4021 ggttttgtgt cccaccaatc aattagtaag ggacaaaaac ttactgaaaa agataaaata
     4081 gacgtagaat tttcatcaga gaatgtagac agcaattcga cgaataattc tgattcaaat
     4141 tcagatgata agaagaaatc tgacagtaaa actgacaagg ataagtcgga ctaacaagaa
     4201 tggctacatt aaattaggat gtattgtcac tatttgtttt tactaaagta atgctgtgat
     4261 agaaaacaac atcttcattt aatctttata aataagtgtt gatagagaat tttaaggtag
     4321 acgaggtcag attaacttgt ttgttatagg caaacttttt atactggcct cttatgccgt
     4381 aaaaaagaaa aattataaat tttgattaca actttgttta tttaattgaa aaattgataa
     4441 tatattagtg gttaaaaaag tctggattaa gaagaggaga tagttatgat ttttgtatat
     4501 gcgttattag cgctagtgat tacatttgtt ttggtacctg ttttaatacc tacattaaaa
     4561 aggatgaaat ttggtcaaag tattcgagaa gaaggtccac aaagccatat gaagaagact
     4621 ggtacaccaa cgatgggtgg actaacattt ctattaagta ttgtgataac gtctttggtg
     4681 gctattatat ttgtagatca agctaatcca atcatactgt tattatttgt gacgattggt
     4741 tttgggttaa ttggttttat agatgattat attattgttg ttaaaaagaa taaccaaggt
     4801 ttaacaagta aacagaagtt tttggcgcaa attggtattg cgattatatt ctttgtttta
     4861 agtaatgtgt ttcatttggt gaatttttct acgagcatac atattccatt tacgaatgta
     4921 gcaatcccac tatcatttgc atatgttatt ttcattgttt tttggcaagt aggtttttct
     4981 aatgcagtaa atttaacaga tggtttagat ggattagcaa ctggactgtc aattatcgga
     5041 tttacaatgt atgccatcat gagctttgtg ttaggagaaa cggcaattgg tattttctgt
     5101 atcattatgt tgtttgcact tttaggattt ttaccatata acattaaccc tgctaaagtg
     5161 tttatgggag atacaggtag cttagcttta ggtggtatat ttgctaccat ttcaatcatg
     5221 cttaatcagg aattatcatt aatttttata ggtttagtat tcgtaattga aacattatct
     5281 gttatgttac aagtcgctag ctttaaattg actggaaagc gtatatttaa aatgagtccg
     5341 attcatcatc attttgaatt gataggatgg agcgaatgga aagtagttac agtattttgg
     5401 gctgttggtc tgatttcagg tttaatcggt ttatggattg gagtgcatta agatgcttaa
     5461 ttatacaggg ttagaaaata aaaatgtatt agttgtcggt ttggcaaaaa gtggttatga
     5521 agcagctaaa ttattaagta aattaggtgc gaatgtaact gtcaatgatg gaaaagactt
     5581 atcacaagat gctcatgcaa aagatttaga atctatgggc atttctgttg taagtggaag
     5641 tcatccatta acgttgcttg ataataatcc aataattgtt aaaaatcctg gaatacctta
     5701 tacagtatct attattgatg aagcagtgaa acgaggtttg aaaattttaa cagaagttga
     5761 gttaagttat ctaatctctg aagcaccaat catagctgta acgggtacaa atggtaaaac
     5821 gacagttact tctctaattg gagatatgtt taaaaaaagt cgcttaactg gaagattatc
     5881 cggcaatatt ggttatgttg catctaaagt agcacaagaa gtaaagccta cagattattt
     5941 agttacagag ttgtcgtcat tccagttact tggaatcgaa aagtataaac cacacattgc
     6001 tataattact aacatttatt cggcgcatct agattaccat gaaaatttag aaaactatca
     6061 aaatgctaaa aagcaaatat ataaaaatca aacggaagag gattatttga tttgtaatta
     6121 tcatcaaaga caagtgatag agtcggaaga attaaaagct aagacattgt atttctcaac
     6181 tcaacaagaa gttgatggta tttatattaa agatggtttt atcgtttata aaggtgttcg
     6241 tattattaac actgaagatc tagtattgcc tggtgaacat aatttagaaa atatattagc
     6301 agctgtgctt gcttgtattt tagctggtgt acctattaaa gcaattattg atagtttaac
     6361 tacattttca ggaatagagc atagattgca atatgttggt actaatagaa ctaataaata
     6421 ttataatgat tccaaagcaa caaacacgct agcaacacag tttgccttaa attcatttaa
     6481 tcaaccaatc atttggttat gtggtggttt ggatcgaggg aatgaatttg acgaactcat
     6541 tccttatatg gaaaatgttc gcgcgatggt tgtattcgga caaacgaaag ctaagtttgc
     6601 taaactaggt aatagtcaag ggaaatcggt cattgaagcg aacaatgtcg aagacgctgt
     6661 tgataaagta caagatatta tagaaccaaa tgatgttgta ttattgtcac ctgcttgtgc
     6721 gagttgggat caatatagta cttttgaaga gcgtggagag aaatttattg aaagattccg
     6781 tgcccattta ccatcttatt aaagggtgtg agtattgatg gatgataaaa cgaagaacga
     6841 tcaacaagaa tcaaatgaag ataaagatga attagaatta tttacgagga atacatctaa
     6901 gaaaagacgg caaagaaaaa gatcaaaggc tacacatttt tctaatcaaa ataaagatga
     6961 tacatctcaa caagctgatt ttgatgaaga aatttacttg ataaataaag acttcaaaaa
     7021 agaagaaagc aatgataaaa ataatgattc tgcttctagt catgcgaatg ataataatat
     7081 cgatgattct acagactcta atattgaaaa tgaggattat agatataatc aagaaattga
     7141 cgaccaaaat gaatcgaatg taatttcagt cgacaacgaa caacctcaat cagctcctaa
     7201 agaacaaaat agcgactcga ttgatgagga aacagtaacg aaaaaagaac gaaaaagtaa
     7261 agtaacacaa ttaaagccat taacacttga agaaaagcgg aagttaagac gtaagcgaca
     7321 aaagcgaatc caatacagtg ttattacaat attggtattg ttgattgctg ttatattaat
     7381 ttacatgttt tcaccactta gtaaaattgc gcatgtaaat ataaatggaa ataatcacgt
     7441 tagtacttca aagataaaca aagttttagg tgttaaaaat gattcaagga tgtatacgtt
     7501 tagtaaaaaa aatgctatta atgatctcga agagaatcca ttaatcaaaa gtgttgagat
     7561 acacaagcaa ttaccaaaca cattaaacgt agatatcaca gaaaatgaaa ttattgcttt
     7621 agtgaaatat aaaggtaaat atttaccttt attagaaaat ggtaaattgc ttaaaggttc
     7681 aaatgatgtc aaaattaatg atgcacctgt catggatggt ttcaaaggta caaaagaaga
     7741 tgatatgatt aaggcgttat ctgaaatgac acctgaagtt agacgatata ttgccgaagt
     7801 gacatacgcc ccaagtaaaa acaaacaaag cagaattgaa ttgtttacga cagatggact
     7861 tcaagtaatc ggtgatattt cgacgatatc taagaaaatg aaatattatc cgcagatgtc
     7921 acaatcatta tcaagggata gttcgggtaa actaaaaaca cgaggctata ttgatttatc
     7981 agtcggtgct tcatttatcc cataccgtgg aaacacgtct agtcaatcag aaagcgataa
     8041 aaatgtgact aaatcatctc aagaggaaaa tcaagcaaaa gaagaattac aaagcgtttt
     8101 aaacaaaatt aacaaacaat caagtaagaa taattaaaaa attttgatat tgtctatgtt
     8161 tatagttcac aagccattca acgtattgta aactaaggat agtgtatttt tttaatagta
     8221 atttgtcagg aggtgcctat ctatggaaga acattactac gtaagtattg atattggatc
     8281 atcaagcgta aaaacaatag taggcgagaa atttcacaat ggtataaatg tgataggtac
     8341 aggacaaacc tacacgagcg gtataaaaaa tggtttaatt gatgattttg atattgcgcg
     8401 acaagcaatc aaagacacaa ttaaaaaggc atcaatcgct tcgggtgttg atattaaaga
     8461 agttttcctg aaattaccta tcattggaac ggaagtttat gatgaatcaa atgaaatcga
     8521 cttttatgag gatacagaaa tcaacggttc acatatcgaa aaagtattag aaggtattag
     8581 agaaaaaaat gatgtgcaag aaacagaagt aattaatgtg ttcccgattc gttttatagt
     8641 cgataaagaa aatgaggttt cagaccctaa agaattaatt gccagacatt cattaaaggt
     8701 tgaagcaggc gtaattgcta ttcaaaaatc gattttaatt aatatgatta aatgcgtaga
     8761 agcatgtggt gttgatgtat tagatgttta ctctgatgca tataactatg gttcaatcct
     8821 aacagctact gaaaaagagt taggtgcatg tgtcattgat attggtgaag acgttacgca
     8881 agttgctttt tatgaacgcg gtgaattagt agatgctgat tctatcgaaa tggcagggcg
     8941 tgatattaca gacgatattg cacaaggatt aaacacttct tatgaaactg ctgaaaaagt
     9001 taaacaccaa tatggtcatg cattctatga ttctgcttca gatcaagata tcttcactgt
     9061 tgaacaggtt gatagtgatg aaacagtaca gtatactcaa aaagatttga gtgactttat
     9121 tgaagcgcgt gtagaagaaa tattcttcga agtatttgat gttttacaag atttaggatt
     9181 aacaaaagta aatggtgggt ttattgtaac tggtggatct gcaaacttac ttggcgtaaa
     9241 agaattatta tcagatatgg taagtgaaaa agttagaatt cacacgccat cacaaatggg
     9301 aattagaaaa cctgaatttt cttcagcaat ttctacaatt tctagtagta tcgcttttga
     9361 tgagttatta gattatgtta caattaatta tcatgataat gaagaaactg aagaagatgt
     9421 tattgatgtg aaagacaaag ataacgaatc taaattaggc ggatttgatt ggtttaaacg
     9481 taaaacaaac aaaaaagata ctcatgaaaa tgaagtagag tcaacagatg aagaaattta
     9541 tcaatcagaa gataatcatc aggaacataa acagaatcat gaacatgttc aagacaaaga
     9601 taaagataaa gaagaaagta aattcaaaaa actaatgaaa tctctatttg aatgattatt
     9661 ggccaataaa actaggagga aatttaaatg ttagaatttg aacaaggatt taatcattta
     9721 gcgactttaa aggtcattgg tgtaggtggt ggcggtaaca acgccgtaaa ccgaatgatt
     9781 gaccacggaa tgaataatgt tgaatttatc gctatcaaca cagacggtca agctttaaac
     9841 ttatctaaag ctgaatctaa aatccaaatc ggtgaaaaat taacacgtgg tttaggagca
     9901 ggagctaatc ctgaaatcgg taaaaaagct gcagaggaat ctcgtgaaca aattgaagat
     9961 gcaatccaag gtgcagacat ggtatttgtt acttctggta tgggtggcgg aactggtact
    10021 ggtgcagcac cagtcgttgc taaaattgca aaagaaatgg gcgcattaac tgttggtgtt
    10081 gtaactcgtc catttagttt tgaaggacgt aaacgtcaaa ctcaagctgc tgctggagta
    10141 gaagctatga aagctgcagt agatacatta atcgttatac caaatgaccg tttattagat
    10201 atcgttgaca aatctacgcc aatgatggaa gcatttaaag aagctgacaa cgtgttacgc
    10261 caaggtgtac aaggtatctc agacttaatc gctgtttctg gtgaagtaaa cttagacttt
    10321 gcagacgtta agacaattat gtctaaccaa ggttctgcat taatgggtat tggtgtttct
    10381 tctggtgaaa atagagcggt agaagctgct aaaaaagcaa tctcttctcc attacttgaa
    10441 acatctatcg ttggtgcaca aggtgtgctt atgaatatta ctggtggcga gtcattgtca
    10501 ttatttgaag cacaagaggc tgctgatatt gtccaagatg ctgcagatga agacgttaat
    10561 atgattttcg gtacagttat taatcctgaa ttacaagatg agattgttgt aacagttatt
    10621 gcaactggtt ttgatgacaa accaacatca catggtcgta aatctggtag cactggattc
    10681 ggaacaagcg taaatacttc tagcaatgca acttctaaag atgaatcatt cacttcaaat
    10741 tcatcaaatg cacaagcaac tgatagtgta agtgaaagaa cacatacaac taaagaagat
    10801 gatattccta gcttcattag aaatagagaa gaaagacgtt caagaagaac aagacgttaa
    10861 tcggttaata tatatacaca aataattcaa cacaaatcat cagataacat atctgatgat
    10921 ttt
//
LOCUS       DAKTLH010000086        10837 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.086, whole genome shotgun sequence.
ACCESSION   DAKTLH010000086 DAKTLH010000000
VERSION     DAKTLH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10837
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.086"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..603
                     /locus_tag="O0394_001818"
     CDS             <1..603
                     /locus_tag="O0394_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCY0499891.1"
                     /translation="FVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLNDRA
                     AHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEGLS
                     AMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIASPR
                     IGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            940..2148
                     /gene="gltS"
                     /locus_tag="O0394_001819"
     CDS             940..2148
                     /gene="gltS"
                     /locus_tag="O0394_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCY0499892.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(2196..3245)
                     /locus_tag="O0394_001820"
     CDS             complement(2196..3245)
                     /locus_tag="O0394_001820"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCY0499893.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRNLNIHRG"
     gene            complement(3276..4223)
                     /gene="corA"
                     /locus_tag="O0394_001821"
     CDS             complement(3276..4223)
                     /gene="corA"
                     /locus_tag="O0394_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCY0499894.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            4320..4811
                     /locus_tag="O0394_001822"
     CDS             4320..4811
                     /locus_tag="O0394_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCY0499895.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQSTITWPEQLGHKIAIPHKG
                     ALK"
     gene            4808..5227
                     /locus_tag="O0394_001823"
     CDS             4808..5227
                     /locus_tag="O0394_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499896.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(5335..6255)
                     /locus_tag="O0394_001824"
     CDS             complement(5335..6255)
                     /locus_tag="O0394_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0499897.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            6463..7101
                     /locus_tag="O0394_001825"
     CDS             6463..7101
                     /locus_tag="O0394_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499898.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADVFKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            complement(7347..9278)
                     /locus_tag="O0394_001826"
     CDS             complement(7347..9278)
                     /locus_tag="O0394_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCY0499899.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNK
                     DEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILIGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFP
                     TFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMHELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQKLDADNSINHE"
     gene            complement(9291..9938)
                     /locus_tag="O0394_001827"
     CDS             complement(9291..9938)
                     /locus_tag="O0394_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCY0499900.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVAIVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            10059..10610
                     /locus_tag="O0394_001828"
     CDS             10059..10610
                     /locus_tag="O0394_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0499901.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKHNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQN"
BASE COUNT         3553 a         1874 c         1621 g         3789 t
ORIGIN      
        1 ttcgttaata atgatacaag acaaattgct aaaaacttat taggtgtcaa agtgatttat
       61 caggatacca ctcaaacgta tacaggctac atcgtggaaa cggaagctta cttaggtttg
      121 aatgatcgtg cggctcatgg ctatggcggt aaaataacac ctaaagtcac gtcattatat
      181 aaacgtggtg gtacaattta tgcacatgtc atgcatacgc atttactcat taattttgta
      241 acaaaatctg aaggtatacc tgaaggcgta cttatccgcg caattgaacc agaagaaggt
      301 ttatccgcta tgttccgtaa cagaggtaag aaaggctacg aggtaacgaa tggcccagga
      361 aaatggacta aggcatttaa cattccacgg gctatcgatg gcgctacgtt aaatgactgt
      421 agattgtcta ttgatactaa gaatcgtaaa tatcctaaag atattattgc tagtccacga
      481 atcggtattc caaataaagg tgattggaca cataaatctt tacgttacac agtgaaaggt
      541 aatccatttg tgtctcgcat gcgtaaatca gattgtatgt ttcccgaaga tacttggaaa
      601 taaatgccat ctttcattga ttactatcat gaaaatgaaa tctatctcct tataagtcaa
      661 tcaatcgtgc cgtcaacatg cggatgggtt gattgttttt ctttgtatcc atcatatttt
      721 ttgattcatc tcctcttatt gaacttgttc ttaattataa aatataacaa tagaattatt
      781 tataattatt aaatttagat gcattaatat tattgatatt attttcaaaa actagaaata
      841 ttgatttgtt gcatgtataa tgttaaaagc gcccttttat aacgcttaca tataaaagct
      901 tatttaggga gagggatatt caacaagggg gatttgaaaa tgatagaact taatgcaatt
      961 acaacattat gtttagcttg tatcctttat ttacttggta aggctatcgt taatcacgtt
     1021 aattttttaa aacgtatttg tataccagca ccagtgattg gcggcttaat ctttgctatt
     1081 ttagttgcgg ctttggattc atttggcatg gttaagatta aattagatgc ttcattcatt
     1141 caagatttct tcatgttagc attctttacg acaatcggtc ttggtgcatc attgaaatta
     1201 tttaaattag gtggcaaagt cttgctatta tactttatgt tttgtgctat catttcagtc
     1261 attcaaaaca tagttggtgt atcactagca aaagtattaa atattaaacc tttgttagga
     1321 ttaacagcag gttccatgtc tatggaaggc ggtcatggta atgctgctgc ttatggtaag
     1381 acaattcaag atttaggtat tgattcggca ctgacagcgg ctcttgcagc tgcaacttta
     1441 ggtcttgtat ttggagggct tatcggtggt ccagttgtaa aatttttaat caaacgttat
     1501 aacttaaagc ctcaacatag tgacgataca tttaaagatt atagccaagt agcatataac
     1561 gaacatttac atagtaaatt taatgccact gaagtattct tcattcaatt tacaatcgtt
     1621 gtattctgta tggcagttgg aagttatttc agtcatttgt ttacagctca aacagggatt
     1681 aatgttccaa tttacgttgg ctcattattt gtagctgtta ttgtccgaaa tatctctgaa
     1741 agttttaatt ttaatattgt agatttaaaa attactaatc aaattggcga tgtcgcatta
     1801 ggtattttct tatctcttgc gctaatgagc attcaattaa tcgaaattta taaacttgct
     1861 atacctctta ttattatcgt tttagttcaa gttgtcgtta tgattttatt tgctgtttta
     1921 attttattta gaggtttagg aaaagattat gatgctgcag taatggtagg tggttttatc
     1981 ggtcatgggc ttggtgcaac gccaaatgcc atggcaaatt tagatgttat tactaaaaaa
     2041 tatggaaact cacctaaagc atatttagtt gtacctattg ttggtgcatt cttaatcgat
     2101 ttaattggtg ttatagtcat tatgggattc atacaatggt ttagttaaac accaaactca
     2161 taaataaaag aggaggcctt cgcctcctct tttatttatc ctcgatgtat attcaagtta
     2221 cgttgttcta tccatgacaa tatttccgga ctaaatacga tttgtttttg tgttaagtcg
     2281 tcaatatttt tagcatctaa catcgtcatt attgatttca tgtgttcaat aaatgattct
     2341 acataagcta ctgtatgtgc aatgccatta ttttcaactt gatttaaaaa cggacgtgac
     2401 ataccagttg cctttgcacc aagtgctaaa cttttaattg catcgagtgg tgtacgtaaa
     2461 ccaccactcg cgaaaactga aatttcgctt tgataagccg ttgtttcaag taatgactca
     2521 actgtagact gtccccatga tgataagtaa tccatatctt tatttgcacg acgttcattt
     2581 tcaatatcta caaagttagt accacctttg ccactaacat cgacatactt gacgcctatt
     2641 tgttgtaagt catgcattaa ttctttgctc ataccaaatc caacttcttt tataatgact
     2701 ggaacagaca ctcgtgatac aatcgacgct atattatcta accaagtcac aaattcacga
     2761 ttcccttcag gcataactaa ttcttgagga gaattaacat ggatttgtaa cgcttgtgcc
     2821 tcaagtaatt caactgcttc caaagccttt tctactggta cgtccgcacc aacattgcta
     2881 aaaatcatgc cttcaggatt catttttcgc gcaatcgtaa acgtctcagc catgcgtgga
     2941 tttctcaatg ccgcatgtgt tgatccaact gccatcgcta agccagtttc tcttgcaact
     3001 acagctagct tttcattgat gtttttcgtc cactcgctac cacccgtcat tgcattaata
     3061 taaaccggat atgccatcgt taagtcaggc gtctgtgatg tcaaatcgat atcatttaca
     3121 ttaattgatg ggatagaatg atgcacaaaa cgcatcttat caaaatctga atgcattgcg
     3181 tcagattggg ccattgctat ttcaacatgt tcattttttc tctgttctct ttgaaaatca
     3241 ctcatgatta aacctacctt ttcgtcattt ctaatttaaa accatttttt acgtctgaaa
     3301 aagatgatta atacgacagc tataacaaac attacagcta agcaaatata atatccataa
     3361 tgtaaatgta atgccggcat atttacaaag ttcataccat aaatcccagc tatgaatgtt
     3421 aacggtgaaa atataactga tactaatgtc agtacttgca taatactatt cattctaaat
     3481 gacgtgtatg actcaaaatt ttctcgtatt tcgtttgtca tttcttgagc agtacgaatg
     3541 atattacgtt gcttaatcaa gtggtcatcg atatgttgaa tgtatagcga atgtttatta
     3601 tctataatca aatcaccatt ttgtttcatt gtatcaatta gctcttgcat aggaaacagt
     3661 acacgtttta ctttaatcaa atccgaacgt aacttaaaga cactatccat gaccatttta
     3721 ttaaagcgat catctacatg gcggtcttca aaatgataaa cactatcttc aagtgcatat
     3781 acaaagttga aatatttatc aaccatcata tctaaaatta atatgacgac atctgcacaa
     3841 tctaattctg catctaatgt attcatatac ttatagacta ctttatttaa tgattccaac
     3901 gtttgatgat gatatgttac taatacattg tcttgtataa aaatatttag tgctattggt
     3961 gaatagtttg accccataat actatggaat actaagtatt gataatcttt ataagattta
     4021 tatttagctc gtggcatacc gttaattgca tcatccactt ctaaatcatt aaaattaaaa
     4081 tgtgctttaa accattcatt ttcttgttca ttcggttcat caaaatcata ccaaacaata
     4141 gtcgcatctt ttggtatctc tttgatatca tcaactactt taaacggttc atatgtagtt
     4201 tgataccgta tctttaaagc catcgatact ccccctaaat aacgaattct ctattatttt
     4261 atcatgaatt aaataacgtg tatgtcttaa tttattttag tatgatagtc actaaggaga
     4321 tggttattat caaacaactt tttacacata ctcaaaccgt aacatctgaa ttcattgacc
     4381 ataacaatca tatgcatgat gcaaattata atatcatttt tagtgacgtc gtgaatcgtt
     4441 ttaattacag ccacggtctt tctttaaaag aacgcgaaaa tttagcatat acgctattta
     4501 cactagaaga acatacgaca tacctctcag aattgtctct tggcgatgta tttactgtta
     4561 ctttatatat ttatgattac gattataagc ggttgcattt atttttaaca ttaactaaag
     4621 aagatggtac actagcatca acaaatgaag taatgatgat gggaattaat cagcacacac
     4681 gtcgttctga tgcttttcct gaatcatttt caacacaaat agcacactat tataaaaatc
     4741 aatcaactat cacttggcct gaacaattag gacataaaat agcaattcca cacaaaggag
     4801 cattaaaatg acagatgcat tacaacaaaa gattcatatc gaattactag atttattaga
     4861 tgatgttaag tttgaattaa cagaattaaa tgcacaaaaa gggttataca ttaacggacc
     4921 agcaaatcag ctacttaagc gtggcgtgca tatggcttat gttcaaggac aaaagcaagc
     4981 catcgataat attatgacta ttgtggaaca acagcttgaa gatcaacatt tcctagaaca
     5041 ttatgataaa tttcaaaatg aggttgctca tcgcaattat gataaaacag ctaactttgc
     5101 agaacttagt gatattccac gtcaattcga taatttttta gatcaatttt atcaaattaa
     5161 agggcaatac tttatcatca cacatatcaa tacacttatt ggtgattttc actcagaagc
     5221 tcattaacaa ttagtctata taacccttgc tatattttca aaaacaaaac ccaattacgt
     5281 tttcatgtca aatatcatct tgcatgaaat cgtaactggg tcatttatat gttattagtt
     5341 attttgtgtt acatcctcat ctatcgattt ggcaatttgt ttaatagctt tatgtgattg
     5401 tctaattgga taaattggaa aatcatgtac catcttagga taatcataaa actcaatgta
     5461 ttgatgatgt tgcaacatca tttgttcaaa tagcttcata tcaggatgtg tcatttcacg
     5521 tccaccacca aacatataaa ctggtggcaa tccttctatt gtgccattaa ttggcgatat
     5581 gcgcttatct gttaatggta ggccattcgc ccattttttc ataatctcat tgacaccaaa
     5641 ctgacttaga accgcatctt gttcgattaa ggcgtccgaa atatctttat tagatagtgt
     5701 tgcatctaaa attggtgaga ttaaatacaa tttattcggt aatggctgtt gattatctaa
     5761 aagagattgt acaaaggata atgccagtgc accacctgaa ccatcaccca tgactacgac
     5821 attttgatgt cctacttcag atactaattg atcataaaca cgttgtatcg cttggaaagt
     5881 atcgtcaata tgaaactctg gtgtctttgg atagataggc agtacaacct catataatgt
     5941 acttaaagtg attttatcca acaatctcca atggaacggt gatggttgta gtgcattgaa
     6001 tccaccgtga atatataaaa ttttcttatc aatttgatgt ctgaaattaa agcgaaagac
     6061 ttgcatatca tctaatgaca atttttctaa atttgcttta acatttaatg ttgaaggctg
     6121 cttatgtttt tttctatttt caatttctct tttataaaaa aatctttcaa catcttgatc
     6181 atttttaaac ataatcgagc gattgtgaag caaatattta ttgacaacgc tattcataac
     6241 acggtttcta atcaatgtct taacctacct ttatatattt tatgtatcca atgattgtct
     6301 atcccctaca ttctttgcca aaaaaagtat ataatgtaga agatattttc tttttcactt
     6361 tcaaatttaa gactacaatt gaacagtgat ttttcatcat tataacagac aactagacat
     6421 attgataagt aaagaaaaga actttatacg gaggtacctt gcatgacaaa tccaaatcaa
     6481 cgattagaac catttgatga gacatttcaa caaccgaata ttcatcgtgg taagcgatat
     6541 ggtaagaaaa aacgttcatt ggtaagcatg attattcaaa tcattgttgt tatattaacc
     6601 gcaatcgctg gatacagcat gtggaaacaa cccattttta atattgtctt caccaaagaa
     6661 tctgtagatt ttgatggcat taaaaatttt aacgagacaa ttggaaaaat gcatcaatta
     6721 aatattaatt tgggtaatat caatgattta caacaaggaa tcgatcgctt aattttagtc
     6781 tttaacggtt tctttatttt atgtatcgtt agcctagtca taacaatact gacaattgta
     6841 tttaatcgca cagttctaaa agtagtaaat attttagtgt taacaattat gttagtcatt
     6901 acattgtatt tcagttatat tattaaagca ctcgcacaag aaattgcaga tgttttcaaa
     6961 aaatattatt taaatgtacc accagatcaa gtgattacag aagctgacgc tattcataac
     7021 ggcatcattt tacttggctg tagcatcggt ttacttgtta taagcttatt cttccgtaat
     7081 cgcatgcctc gtttaaaata atgttatata gtagggattg ggacagaaat gttattttca
     7141 caaaattcat ttcgttgacc cacttcctgc tttttcttta ttaatgacct actacgtcaa
     7201 tgccaaattt taatataatt cctctaacat tctataaatc aacaaataaa aaaagtatcg
     7261 ataaccatat ttggcaccga tactttaaaa atcatgtccc cacaacataa tttttgatta
     7321 gtcacacttc aattaattta ttttatttat tcatgattga tactattatc tgcatctaac
     7381 ttttgagctg tagcttttgc ttttttatta ctttgtaaaa ataaacataa aattaaggcg
     7441 atgatgctaa atatcgttgc aactataaat gcatcattaa taccttcaac cgttgctagt
     7501 ttatttacaa attgtagtaa cactttcatt gcgccttctt gtccgccata ttgtgatgct
     7561 aattcatgca tatgatcttg tacaacagga ttcgttttat ctaactcttc cccaaaagct
     7621 gataagtgtt gtgtagtttg tgttgtcatt acagtaacta agattgctgt acctatagag
     7681 cctgctaatt gacgcatcgt atttaagaaa gcattaccat gagaggcaag tcgtcccggt
     7741 aacgcattaa tagctgcagt taccattggc atcattataa atgccatacc aaatgaacga
     7801 agtacataga tacccatgat tgtcatatat ggtgtatcca tatttaattt agttaattcc
     7861 catgttgcat aagtcattac agcaatacca aagatagcta atggttttaa accaatagta
     7921 tctaacaatt tacctgcaaa tggtcctagt agacccataa ttagagaacc aggtaataat
     7981 aacaatccgg aatctaatgc tgagaatccg cgtaaatttt gtaaataaat cggtaataaa
     8041 atcataccac catataaact taacattaca accatattaa taattgttgt taatgtaaat
     8101 gttgggaatt tcaatacttc taaattcaac attggtgatt tcattcttaa ttctctaata
     8161 acgaatagaa taataaagat aataccaatc gcaaacattg tttctatctc tactgaaccc
     8221 caacctttgt tgccagcttc tgagaaacca tataacaaag caccaaaacc aatcgtacta
     8281 aaaatgatac ctgggatatc agctttaggg tttgttgtat attgatataa cttaaaccat
     8341 acaaaaccaa ttaaaatagc gataatcccg ataatgaaca taccgtaaaa catcacattc
     8401 caatggtaat tttgtacaat ataacctgat aatgttggac caattgcagg tgctaaaatc
     8461 attgcgatac ccattgtacc catggcagca ccacgttttt caggtggata aattgtaata
     8521 ataacaattg aacctaatgg cattagtaca cctgcaccaa tggcttgtaa tacacgtcca
     8581 accatcatga ttgggaaatt cattgaaatc gcacagatta atgaaccaat tgtaaagagt
     8641 actaacgcaa ctaaaaataa ttttcgatat gaatatttat taaatagata cgccgtaatt
     8701 ggtattaaaa taccgtttac taacatgaat cccgtcatca accattgccc tgttgacgca
     8761 gaaatattaa attccgtatt aatttttggt aaagcaacat ttaataatgt ttggtttaaa
     8821 atcgcaataa acataccgaa taataatgcc gctaatattt taccgcgtga aacaccttca
     8881 ccaaaaataa agtttttatg ttcttttttt attttttcat tcactttata ttcttctgat
     8941 tcaggatttt tagcagcaac tgcttcctca tccttattat tagtgaatgc ttcttgatct
     9001 ttctcagacc cctttgtgta accatttaga ctaacttggc tatgatcatc ttgattgtca
     9061 acacgcaact cttcatgcgt catacgttgt gtcgattgat cagttgtttt gtctaaatca
     9121 ctagctttaa atttagattg atttgattga cgtgtcgtaa attgttgttc cttttgttgg
     9181 cgtttgcctt tttttcttga tcttattaaa aataaattga taaccccaac aataatgagc
     9241 gctaaaataa tgtagctaat aatgaaggtc gtagtcattt aatgaccccc ttaattttta
     9301 tggattttta cttcagcgtt cattccagga acaacttgtt tagacggttc tgattctaga
     9361 gtgattttaa caggtattac ttgagaaact ttagtgtagt taccatcact atttgatgat
     9421 ggcattaatg aaaagcttgc agcagttgct tttccaatac tatcaacttt acctttaata
     9481 gaagcttttt gaccgtcaat agtcacatca acatctttac ctacttcaac atctttaata
     9541 tctttttcgt caatatttgc tgttacatat aaatcatcta aattgtatgc ataagcgatt
     9601 gggttaccag cttgcaccat tgaaccttcc ataccatcta atttggcaat tgtacctttt
     9661 tgaggcattt taagatccat atctttcgtt tcgccatctt gaccttgtac agtaacaatt
     9721 gctactttgt cacctttatc gagtttgtca ccttgtttaa cattaagtga tttaatttgt
     9781 ccagatgcag gacttgctat tttaatttga tcgccattta cttttgcatt atcagttgtt
     9841 acatagcttg ttgttttatt ccaaaaataa aagccagcaa tcccaattgc taacagtaca
     9901 acaaccgtaa tgacattaat taatatcatc tttttcatga aacatttcct cctatttgct
     9961 tttaaaattc actttattta ttatagagac atgataacta aaaaaacacc aacaattttt
    10021 ataagagtgt tggttaaaat tcacaaaggt gtaacaatat gaaacgacaa gccaaaatag
    10081 aaattcaaaa cgcacttgtt gatttgatgg ctgaatatcc ttttcaagaa atatccacta
    10141 aaatgatttg cgcgtattgt aatattaatc gttctacttt ttatgattat tacaaagata
    10201 aatttgattt attggatacc atcaactcaa agcataagga aaaattccaa tttcttctaa
    10261 gcgcgctaca tcataatttc gaaaatatta aacaggataa gcttaaactt tataaattct
    10321 tcattattat tgctaagtat atcaagcaca atgagcagtt tttcaaagac attttagtta
    10381 cctatccaat gaaaacatta ttcatagact atataaattt agcgagagat tactatcaac
    10441 aaattatgaa tgactatagt actacagtaa ccaataaaca gctttatgtt acatatatca
    10501 taggcggtca agctggggtg tttattaatt ggctttcgaa tggttgtaat gaatcacctg
    10561 aagaagttgc tgacatttta ttggctaata cgattaaact tcaaaactaa taatacggtt
    10621 tcattctaat aaagaaatgc tataaaacaa ctagataaag ttaaaccaaa tggatagata
    10681 atagtacata tgtaacggtg ctatcaatat aaaaatgccg agatttccac ataataatct
    10741 cggcattttt atttttgatg ctaaattcat aggtctatga atagctcaag ttttgttttt
    10801 taatttttca attcaatata atagaagaaa ctatgtt
//
LOCUS       DAKTLH010000087        10537 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.087, whole genome shotgun sequence.
ACCESSION   DAKTLH010000087 DAKTLH010000000
VERSION     DAKTLH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10537
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.087"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(218..430)
                     /locus_tag="O0394_001829"
     CDS             complement(218..430)
                     /locus_tag="O0394_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499902.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQEQI"
     gene            complement(460..1071)
                     /locus_tag="O0394_001830"
     CDS             complement(460..1071)
                     /locus_tag="O0394_001830"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCY0499903.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKLAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            complement(1071..1763)
                     /gene="pyrF"
                     /locus_tag="O0394_001831"
     CDS             complement(1071..1763)
                     /gene="pyrF"
                     /locus_tag="O0394_001831"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCY0499904.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQDTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQNDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            complement(1873..5046)
                     /gene="carB"
                     /locus_tag="O0394_001832"
     CDS             complement(1873..5046)
                     /gene="carB"
                     /locus_tag="O0394_001832"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCY0499905.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            complement(5039..6139)
                     /gene="carA"
                     /locus_tag="O0394_001833"
     CDS             complement(5039..6139)
                     /gene="carA"
                     /locus_tag="O0394_001833"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCY0499906.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            complement(6141..7415)
                     /locus_tag="O0394_001834"
     CDS             complement(6141..7415)
                     /locus_tag="O0394_001834"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCY0499907.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            complement(7433..8314)
                     /locus_tag="O0394_001835"
     CDS             complement(7433..8314)
                     /locus_tag="O0394_001835"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCY0499908.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            complement(8342..9649)
                     /locus_tag="O0394_001836"
     CDS             complement(8342..9649)
                     /locus_tag="O0394_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCY0499909.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            complement(9867..10394)
                     /gene="pyrR"
                     /locus_tag="O0394_001837"
     CDS             complement(9867..10394)
                     /gene="pyrR"
                     /locus_tag="O0394_001837"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCY0499910.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
BASE COUNT         3144 a         2050 c         1571 g         3772 t
ORIGIN      
        1 caaatttgat tacaattaaa attaagttaa aggagggacg ttatgttaca ttatgtgatt
       61 ccagtattag tcctattact cgtattctta attggttgga ttttaataaa gttaattatc
      121 cgctaataaa ttttttatat aataaaagct atagtgtttg taatattcgg atgcggttaa
      181 gcagcccctg atattaaaca ctatagcttt tttgttttta aatttgttct tgtttacgta
      241 atatatcctt tacatgttta aataaatcat cattatgatc tatagttgtt ttatttttta
      301 tatgtttttg cttcgttgaa ctttcgtcga catcgtgact actagataga ttggaatcat
      361 ttgaatgatt gataaattcg ttacaatgtt ttatcttccc atataaatca ttagatgttt
      421 tgtcattcat ttgttgaatt cctccttcta gtgtctatat tatgctaagt tgtctctcca
      481 ttcaactaat gtttggatat cttcactaga aattttacct tcattttcag ctacttcaat
      541 taattcattg taatcactta aagtgtaaaa aggtagttga atattgctaa atgtatcatc
      601 tgcttttttc aaaccgtaag taaagatagc tacaacacct aatacttctg cacctgctag
      661 ttttaaggct tcaactgctg tgactgaaga tccccctgtc gaaattaaat cttctatcac
      721 aactactttt ttaccttcac ttttagcacc ttcgatttga ttttgcttac catgactctt
      781 atttgatgaa cgaacataat tcattggtaa ttttaatttt tcagcaataa aagctgcatg
      841 tggaatacca gctgttgcag taccagaaat aacttctact tcaggaaagt gttctttaat
      901 taagttaatt aaaccatcgc ggattgcgcc tcgaactaaa ggataaccta acgtaacacg
      961 gttatcacag taaatcggtg atttaatacc tgaactccat gtatataaat catttggtga
     1021 taatgttaca gcttcaatat ctaataatga ttttgcaatt tctttagcca ttatactaac
     1081 caactttctt taattttatg ataactttcg actggattgt cactttgtgt aatcggtcta
     1141 ccgactacaa tatgcgtcga accaagctgt cttgcttctt ccggtgtcgt aatacggtgt
     1201 tggtcatttt gagatgcacc tttaggtcta atacctggtg ttacttttaa aaatgatgta
     1261 cctaactttt cagtcaacat acgactttca agaggtgaac aaacaacgcc atctaaacca
     1321 gctgcatttg ctaacttggc ataatttaaa acggcctctt caatcgatgt ttgtatattt
     1381 tgttcgtgtc gtaacatgtc ttctgttgtt gacgtaagct gtgttactgc aataattttt
     1441 gtatcttgat tatgttttct taatccttta atggcctcag acatcatttt tacgccacca
     1501 gcagcatgaa catttaccag atcaacattc aatttagcta gtccttccat cgccttacca
     1561 actgtattag gaatatcatg cagttttaaa tctaaaaata catcatggcc tctttctttt
     1621 atctcattaa ttaattgagg accttcttga taaaaaagtt ccatacctac ttttacgaat
     1681 aatgattcat caaataaatc taaaaattga tttacttttt cttttgattc aaaatctaat
     1741 gcaataattg gtaaatcttt catcatatat gtctcccttt atattaagaa attcattaaa
     1801 ataatgaggc tgtaatataa acttctttaa gtaaatggtt aattacagcc ccaccgatac
     1861 agtttgattg atttacattt gacgcattgt aaatgtcata ctttcaatta cattcgttaa
     1921 ggcattagct gtatctaaag atgtcaaaca tggaatacca ttttcaactg tagtacgtct
     1981 aatttggaag ccatcccttt ctacttcttt acctttagtc attgtattta taacgatttg
     2041 aacatcacca ttttgaatac gtgttaataa atcattttcg ccaccaattt tgcctactac
     2101 ttctgcaggt atgtcatact cagctaattt attagctgtt ccagacgttg ctaaaatttt
     2161 atagccaact tcatttaagc gttgtgccaa tttaacaact tcctctttat ctttgtcact
     2221 gacggtcatt aatactgtac cgtgatcttt aacttcaacg ccactacctg ttaacccttt
     2281 gaataacgcc ttttctaatg tagtatcttt ccccatcact tcacctgttg acttcatttc
     2341 aggtcctaaa gtaatatcaa catttttcaa tttattaaaa ctaaatactg gtgctttcac
     2401 aaagacaccc tcagcatatg gttgaacccc ttcttgataa cccatatctg ttagtttttc
     2461 cccaatgatt gctcgcatag ctaattgtgc cattggaata tcagtaattt tacttaagaa
     2521 tggtaccgtt ctactagaac gtggatttac ttctaaaaca tacacaccat cgtgagctat
     2581 aacgaattga atgttgatta agccaatgat gtttaaacct ttagctaatt ttatagtata
     2641 gtcctcaagt gttgctaact cgtcttctgt caaagtttgt ggtggatata cagcgattga
     2701 gtcaccacta tgcacaccag cacgttcaat atgttccatg attcctggaa taatgaccgt
     2761 ttctccatca caaatcgcat caacttcaat ttctttacca gttaaatatc tatcgactag
     2821 taccggatgt tccggactcg cttttacagc ctgggtcata tagttttcta actctttgtc
     2881 attgtctaca atttccattg cgcgaccacc taatacataa gaaggtctta ctacaaccgg
     2941 atatccgatt tctgcagcat tcgctaatgc ttcctcaggt gatgtagctg ttttcccttg
     3001 tggctgtggc acgttaattt ttcttaatag tgcttcaaat tcttttctat cttcagcacg
     3061 atttagattt tctagtgaag taccaagtat tttaacacca tgtttagcca atttgtctgc
     3121 taaattaatc gctgtttgtc ctccaaattg tacaacgaca cctttaggtt tttctaaatt
     3181 aatgatattc atcacatctt cttcagttaa aggttcaaag tataatttgt cagaaattga
     3241 gaagtctgtt gaaactgttt ctggattgtt attcacaatt atcgcttcgt accctgcttt
     3301 ttgaattgcc caaacggcgt gaactgtcgc atagtcaaat tctacacctt ggccgattcg
     3361 aattggtcca gagcctaata ctaagatttt ttctttgtca gtaactatgg attcattttc
     3421 agtttcgtat gtaccataat aatatggtgt tgaagattca aattcagctg cgcaagtatc
     3481 aaccatcttg taaacaggtt taatatcatt ttccatacgc aattgatata cttcttcttc
     3541 cgtcatatta aagcgatgcg ctattgtttt atcactaaat ccataatctt ttgcatattt
     3601 aagatattct aaatcacctt gatgctcttt taattgatgc tcaatatcaa taatgttttg
     3661 gaacttgtgt aagaagaagt aatcaatctg agtcatatta tgaatttctt ctaatgttgt
     3721 gcctcttcta attgcttcgc cgatgaaaaa taatcgttca tcatcttggt gtgaaatacg
     3781 ttctttaata taatcaagat cgaagctttc accatttggt aatcctaagt gatgcacacc
     3841 atactcaagt gatcgaattg cttttaacaa tgattcttcg taagttcgac caatggccat
     3901 aacttcacct gttgctttca tttgtgtgcc aagctctcgt tctccttttt caaatttatc
     3961 aaaaggaaat cttggtattt ttgaaatcac atagtctaaa gttggttcaa acgctgcata
     4021 agatgttcct gtaattggat ttaacatttc atctaatgtt agaccaaccg cgattttagc
     4081 agctaatttt gcaataggat atcctgttgc ttttgaagct aacgctgatg aacgtgatac
     4141 acgcggattt acttctataa tataataatc gaatgaatgg ggatctaatg ctaattgaac
     4201 attacaacca ccttcgatac ctaaagctcg aataactttt aatgaaacat cacgtaacat
     4261 ttgatactca acatctgata atgtttgact aggagccaca acaattgaat cgcctgtatg
     4321 aataccaact ggatcaatat tttccatgtt acatacaacg atggcattat cgtttttatc
     4381 acgcattact tcgtattcga tttctttaaa acctgcgata gatttttcta ataaacattg
     4441 cgttgctgga ctataatgaa gaccatttga gacgatttca tgtaattctt catcattatg
     4501 acaaataccg cctccggtac cacccatcgt aaatgccggt ctaacaatta gcgggtatcc
     4561 cacttgctct ttgaatttaa aggcttgctc tactgtattt acaatgtcac tctctggtac
     4621 aggaacgttt aaatcattca ttaatgttct aaacatttca cggtcttctg cttgttgaat
     4681 tgatgttagc tcagttccta ataattggac gttattatct tgaagcacac cactttcgtg
     4741 tagttgaatc gccatgttta aacctgtttg accacctaaa gttggaagta aagcgtcagg
     4801 ttgctcttta cgtataattc gcgctataaa atcatgagtt aacggttcga tatatacttt
     4861 atccgcaatt tccttatcag tcatgattgt cgctggattt gaatttacaa gaataactcg
     4921 atatccctct tcttttaaag ctagacatgc ttgtgttcca gcataatcaa attcagctgc
     4981 ttgacctatg ataattggcc cagaccctat tactaaaatt gttttgatat cattacgttt
     5041 aggcattgat atgacgctcc ttttccttaa aattagtcat cattgctaca aaatcatcaa
     5101 atagatagtt tgaatctgac ggtcctggat ttgcttcagg atggtattga acagaaaatg
     5161 ctggtaatgt tttatgtttt aaaccttcta cagtaccatc atttaatgca agatgagtaa
     5221 cttctaaatc agtacttttt aacgaatcta tatctattgc atatccatgg ttttgactcg
     5281 taatatcaac ttttccagtc tctaaatttt taactggatg gttcgcacca cgatgaccaa
     5341 acttcatttt aaatgagctt gctccttgag ataatgcaaa aagttgatgt cctagacaga
     5401 taccaaagaa cggaattttc cctaaaattc cttgaatcat tggaatcgca cattctacaa
     5461 cttcaggatt acctggtccg tttgatagca taacgccatc tggagccatt gctaaaattt
     5521 cttcggcagt agttgtatat ggtacaactg tgacgttaca acctctgacg tttaattctc
     5581 gaacaatatt ttgcttctta ccaaagtcta caagtacgac acttagatcc ttacctgtcg
     5641 aaacatacgg tgttttagtc gaaacgattt ctacttcgtt tttaggtaat tctacttgtt
     5701 gtaaatgttt gacaagttga tcaatatctt cttttcgatc agtaaaacca gcttttaaca
     5761 caccgtgttg tcgaatttta cgcgtaatac ttcttgtatc aacacctgca atccctggaa
     5821 tttgatgcaa ttctaaaacg tcatgaagtg tcttttgctg tctaaaatta cttggatgcg
     5881 cactcgcttc tttcactaca ataccgttta atgtaggtac taatgattca aaatcgtctc
     5941 tattgatacc ataattaccg attaatggat acgtaaaagt aatgatctga cctgtatatg
     6001 atggatctga aatagtttct tgataacctg tcatcgctgt attaaataca atttctccta
     6061 cagttaagtt atcagatcct aaacggtagc cttcgtaaaa agaaccgtct tctaacacta
     6121 gataacgttt gctttgcata ttatttatcc ccctcaaatt taacttcgcc ttcaaccatt
     6181 gttaagatcg gatttccata aactttatag ccgataaatg gtgtattatc tgcttttgat
     6241 aagaaatctt ctcctttaat ttcttgttca ctatctaaat caatgattgt taaatctgca
     6301 taaccatttt cttttaatgt gccgtattct aaattaaatg tctcacatgg tttaattgtt
     6361 aagtaatcta ctaattgttg taatgtccaa tcaccatttt ttacaaaatg cgtatataat
     6421 aatgggaatg ctgtttcact accaacaatt ccgaatggtg ctttttccat tggttgtgct
     6481 ttttcatcac gtgcatgtgg tgcatggtct gttgcgatac agtcaattgt accgtctagt
     6541 aacccttcta acaaagcctc tctatcttca gtacttctca atggtggatt cattttataa
     6601 atggcattat taccaggaat atcatcttct gttaacaata aatggtgtgg tgtaacttca
     6661 gctgtaacat gaatgcctgc gcgtttagcg tcacgaatga ctctaacact ttctttagta
     6721 gaaacatgac atacatgata atgacaacct gctgcttcag ctaatagtac atctcttgcg
     6781 atttgaacag attcacaaat gtttggaata cctggtatac ctaactcttt actgcgtttc
     6841 ccttcatgca ttgcaccacc atagattaat gaattatctt cacagtgtgc tacgatggct
     6901 ttgtttactt ttgcagcttc aatcatgcct tcatacatca tgcttgcagt ttgtactcct
     6961 acaccgtcat ctgtaaacgc aaaggcacct tcttttacta gtgctgggaa atcaaccaat
     7021 tctttaccta attgacgtgt tgtaattgaa gcataaggta atacacgtac ttgagcattg
     7081 tcatcgatta atttttgtaa agcttcaaaa tgttctacag aatctggtac cggtcttgtg
     7141 ttaggcattg gacatacagt tgtaaatccg cctctagcag cagctttagt accagtttca
     7201 attgtctctt tatattcacc accaggttca cgtaaatgaa catgaacatc gacaaatcca
     7261 ggtgacacaa agtgaccttt cgcatctatg atgtcaacac cattgcttgg ttcaattgca
     7321 ggtgcaattt gtttaattac cttaccatca attaaaatat ctgcttgttg taattcgcca
     7381 ttttgtaata ctttaccgtt tttaattaat ttcatcattt tcgtcccctt acctatttta
     7441 ataattcatc aatgactgcc attcttaagt aaacgccatt ttccatttgc ttaaaaattc
     7501 ttgattttga agcttctact aaatcgcttt gtatttctac tcctctattc acaggtgccg
     7561 gatgcataac aatagcatgt tcttgtaatt tgttatagcg tacttcattt aagccatgct
     7621 tttgatgata atcatctgct gcaaatctag tttcttctgc aagcccatgc ctttcatgtt
     7681 gaattcttaa taacataact atatctactg tttctataac atcatctata tttacataag
     7741 gtgcttctaa agaatcatca atccaagcat ttgggctatt aaacattacg tttgcaccta
     7801 atgcttttaa actatggtaa ttactacgtg cgacacgtga atttttaatg tctccacaaa
     7861 tcaatacatt caagccttca aaatatccat attcttcata tatcgtcatt aaatcaagta
     7921 aactttgtgt tggatgttgt ccactaccat caccagcatt cgcaattggg atgttaatat
     7981 tcgctaattt ttcatagtag ttattaaacg gatgtctaat gactaataaa tcacagccaa
     8041 tactttctaa agttttacat gtgtcatata aagattcacc ttttgaaaca gatgatgttg
     8101 atgtttcaaa gctaatcgtt tttaacccta gcttaagttc tgccatttca aaactacatt
     8161 ttgttcgagt agaattttca aagaataaat ttgcgacata tttcccttca aagtttggta
     8221 gttgacgttc accagattta aattgacttg ccttttggat aagtttgtat atttgatctg
     8281 tagataaatg ttccattgat aataaatgat tcataaaagc gccctcctta atttgtaaat
     8341 cttaattgtt ttgttttttc tctttaggta agattaagtt caaaataatt cctgaaagtg
     8401 cagctaatgc catcccctca atttgaaggt tgataccaat ttcttttaag ttaaatacta
     8461 aattaccgat acctacaact aaaattacag aagctataac taaatttcga ttgttcgcaa
     8521 aatctacttt gctttcaact aacattctta agccacttgc tgcaataata ccgaaaagta
     8581 atatagatac tcctcccata actggtgtag gtatagaaga aattaatgca gtgaacttac
     8641 caatgaatgc taaaacaatt gctataactg ctgcaccacc aatgacgtaa atactgtata
     8701 ttctggtaat cgctaataca ccgatatttt caccgtatgt tgtacttggt ggtccaccaa
     8761 taatactggc aaacattgta gaaacaccat caccaatgat tgatttatca agtcctggct
     8821 tttcaaagaa gtttctacct acgattttat tcaataccat ttggtgccca atatgttcac
     8881 ttactgttac gaacacaatc ggaatcatta caagtacaag tcctaagtga aatgaaggta
     8941 cataatcttt aaatggcaga taaatatgag ggaaatctat ccattttgct gacataattg
     9001 catcaaattt cacaatcccc ataaaaatcg ctaccacata acctacgata atacctacaa
     9061 gaactggaat taaagatagg aatcctttaa aaaatccttg aacgactatt gttacaagca
     9121 aggttatcat tgcaacaatt aagaaactga tattgtaacc tttcatatct ccaggatttt
     9181 catacattgc catattgact gcagtaggcg ctaagcttaa accaattacc atgatgactg
     9241 gtccaacaac aactggtggt aataatttca ttaaccatgc tgtcccactt aatttgatta
     9301 gaatcccgat gatgacgtac ataacaccac tcatgaataa tgctacaagc atgtctccta
     9361 agctatgcgt acttaatccc gtgataattg gcgtgataaa tgcaaagcta gatcccaagt
     9421 atgctggtat ttgcgccttc gttattaaga tataaagtaa tgtaccgatt cccgaagcta
     9481 gtaacgctgc tgatattggt agtcctgtta agaatggtac tagtactgtt gcgccaaaca
     9541 tcgcaaataa atgttgtaag cttaaaaatg cccattgcgc tggttgtggt ttttcattta
     9601 catctagtac gggttttact gttcgttcaa acatttcatc attttgcata atattcattt
     9661 cctccgataa taaaaaaatc tctttacatc agtatatgta aagagacaaa aagtgtgaca
     9721 agttgctaca agtcattttc gtccatagaa attgacttat agttgtcgaa catgagggta
     9781 ttattagata aacaagcata tgaaaactta tttatcattc aactccccca cctttttcag
     9841 tctctcgtac tgaattaaaa ggggtattat ttaattataa ctgcatttct ttgatccatt
     9901 tcttctaaat agacacttac cgtttcctct ttagaagtag gtatattttt accaacaaaa
     9961 tctgctcgaa ttggtaactc acgatgtcct cgatcaacca aagcagctaa accaatttta
    10021 ataggtctag catttagcaa aatagcatca agtgaagcac gaaccgttcg accagtatac
    10081 agcacatcgt caataatgat gactacttta tctgtaatat ctgtgtcgat gtctattgcg
    10141 tcttttgtcg taagtgatga catgtgctct atatcatctc taaagtatgt aatatcaatt
    10201 gttccagtag gtatacgttg ttgctcaatt tgatgaattt tatcttgtat acgattcgct
    10261 aaatattcac ctcttgtttt gatacctaaa agaattaaat tatcagtacc tttgttatac
    10321 tccaaaattt cgtgagcgat tctcgtcact gtacgttgta ttgcggcatc atccattatg
    10381 atacgttcag acatattctc accctttcaa aatttagaaa caaaaaacct catgtcttta
    10441 atatcgacat gaggtttagt atatacaatc agcctattta catagaagaa tcgtagtatt
    10501 actacttatt cttgcatatg tgaaagtgtt atgatca
//
LOCUS       DAKTLH010000088        10365 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.088, whole genome shotgun sequence.
ACCESSION   DAKTLH010000088 DAKTLH010000000
VERSION     DAKTLH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10365
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.088"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            684..1979
                     /gene="purB"
                     /locus_tag="O0394_001838"
     CDS             684..1979
                     /gene="purB"
                     /locus_tag="O0394_001838"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCY0499911.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            2088..2390
                     /locus_tag="O0394_001839"
     CDS             2088..2390
                     /locus_tag="O0394_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499912.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            2562..3254
                     /locus_tag="O0394_001840"
     CDS             2562..3254
                     /locus_tag="O0394_001840"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HCY0499913.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVVFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKMAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            3251..5443
                     /gene="pcrA"
                     /locus_tag="O0394_001841"
     CDS             3251..5443
                     /gene="pcrA"
                     /locus_tag="O0394_001841"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCY0499914.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            5447..7450
                     /gene="ligA"
                     /locus_tag="O0394_001842"
     CDS             5447..7450
                     /gene="ligA"
                     /locus_tag="O0394_001842"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCY0499915.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            7463..8662
                     /locus_tag="O0394_001843"
     CDS             7463..8662
                     /locus_tag="O0394_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HCY0499916.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGSTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(8751..10289)
                     /gene="putP"
                     /locus_tag="O0394_001844"
     CDS             complement(8751..10289)
                     /gene="putP"
                     /locus_tag="O0394_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCY0499917.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIIRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
BASE COUNT         3920 a         1506 c         1910 g         3029 t
ORIGIN      
        1 gaaagtttca tgaaaatata ttagtcgatt ttgaaagaag cgtagtcaat ttatgtcttt
       61 ctataaaaat ttactattaa aaaaatctct gcgtattttt ctataatcag tttcgtattt
      121 atacgattct ggagttcaat attaatgtct tatatttaaa atgccacacc tatgcaatag
      181 aatatatatt ctttttgtga gtagaaaatt tgttatatta gaagcgaata attttaaaac
      241 atattatgaa atgtaagaag aaatattatg aaactaggaa aagtagattg ttgaaaattc
      301 catacaatag gagaagtaaa gtgaataaat tctggtatta ctaaagctat tgattgtcct
      361 aattgtaata aaaagttgga tagtgtagtt gaggggagaa aaactaaaat gcaaaaatat
      421 attctcaatg gatcattata aataccaatt agattaaagt attgcgaaca tttacaatta
      481 gaaatcagta ttaatctgcc atagataatt gtattaatgt aaaaaataaa atagaatgag
      541 aatatacgga aattttttat acatttataa ccaattctgt taaactgtga agagtagagt
      601 taaaaacgaa ctttttgttt atttttatta aaaaatgttc tgtttttgaa aattcgagta
      661 tacatacgat aggagttaaa aaaatgattg aacgctattc tagagaagaa atgtctaata
      721 tttggactga tcaaaatcgc tatgaagcat ggttagaagt ggaaatttta gcatgtgaag
      781 catggagtga attaggacat ataccgaaag ctgacgtaca aaaaattcgt caaaacgcaa
      841 aggtaaatgt cgaacgtgca caagaaattg aacaagaaac gcgccatgat gttgtagcct
      901 ttactagaca agtttctgaa acgctaggtg aagaacgtaa gtgggtacat tatggtttaa
      961 cttctactga tgttgtagat acagctttaa gtttcgttat taaacaagca aatgacatta
     1021 ttgaaaaaga tttagaaaga tttatcgacg ttttagctga aaaagcaaaa aattataaat
     1081 atacattgat gatgggacgt acacatggag tgcatgcaga accaactaca tttggtgtca
     1141 aaatggcatt gtggtacacg gaaatgcaac gtaacttaca acgtttcaaa caagtaagag
     1201 aagaaattga agtagggaaa atgagtggtg cagtaggtac ttttgctaac attcctcctg
     1261 aaatagaaag ttatgtgtgt aaacatttag gtattggcac agcgcctgta tcaacgcaaa
     1321 cattgcaacg cgatcgacat gcatactata ttgcgacatt agcattaatc gcgacatcac
     1381 tagaaaaatt tgccgttgaa atacgtaacc ttcaaaaaac cgaaacgaga gaagttgaag
     1441 aagcatttgc aaaaggccaa aaaggttcat ctgcaatgcc tcataaacga aatccaattg
     1501 gttctgaaaa tatcacaggt atttcaagag tcattcgtgg ttatattact acagcttatg
     1561 agaatgtgcc attatggcat gaaagagata tttctcattc ttctgctgag cgtattatgt
     1621 taccagatgt tacaatagca ctagattatg cattgaatcg tttcacaaat attgtggacc
     1681 gtttaactgt atttgaagat aatatgcgta ataacataga taaaacattt ggtctgattt
     1741 tctcacaacg tgtcttatta gcattaatta ataaaggtat ggttcgtgaa gaagcatacg
     1801 acaaagtaca accaaaagcg atgatatctt gggaaacgaa aacaccgttc cgtgaattaa
     1861 tcgaacaaga tgagtctatt acaagtgttt taacaaaaga agaattggat gaatgtttcg
     1921 atcctaaaca tcatttgaat caagttgaca ctattttcga acgcgctgga ttagcataaa
     1981 attaaataaa gtaacgattc agatttaata aaatataaat cgctacactt taatagatta
     2041 atgcgttaaa atgacataaa gaataaaatt ataggggttg tttcgatatg caaattgaaa
     2101 aattacgagg tgcagcgtta gatgaattgt ttgatgcaat actaacgtta gaaaatagag
     2161 aagaatgtta ccaatttttc gatgatttgt gtactgtaaa tgaaattcaa tcactgtctc
     2221 aaagattaca agttgctaaa atgattaagc aaggttatac ctatgcaacg attgaacaag
     2281 aatctggagc atcgactgca acgatttcta gagtgaagcg ttcattacaa tggggtaatg
     2341 atgcttatac aatgatttta gatcgtatga atattgaaac aaatgaataa taaatgaacg
     2401 ataaacaatg gttatctatc tgcactaata aggtagataa tcattgtttt ttcacgaaaa
     2461 aatttacaga gtaaaagaac ttaaatttca tattaagtct ttagaactcg acacttaaaa
     2521 atgctataat catatgtatg ttaaaaaagg agtttcggaa aatgtatgac attaaaaaat
     2581 ggcgccatat ttttaaatta gacccagcta aacatatttc agatgatgat ttagatgcga
     2641 tttgtatgtc tcaaacagat gcaattatga ttggtggaac tgatgacgtt actgaagata
     2701 atgtcattca tttaatgagc agagtaagaa gatacccatt gcctttagta cttgaaatat
     2761 caaacatcga aagtgtaatg cctggttttg atttttattt tgtacctaca gtactgaaca
     2821 gtacagatgt tgtatttcac aatggtacat tattagaagc gcttaaaaca tatggacata
     2881 gtatagattt tgaggaagta atatttgaag ggtatgtcgt gtgcaatgct gatagcaaaa
     2941 tggcaaaaca taccaaagca aatacagatt taacaacaga agatttagaa gcatatgccc
     3001 aaatggtcaa tcatatgtat cgattaccgg ttatgtatat agagtatagt ggcatttatg
     3061 gcgacgtatc aaaggttcaa gctgtctcag aacatctaac agaaacgcaa cttttttatg
     3121 gtggcggtat ttcctcagaa caacaagcga cagagatggc agctattgca gatacaatta
     3181 tcgtcggtga tattatttat aaagatatta aaaaagcttt aaaaacagta aaaataaagg
     3241 agtctagtaa atgaatgcgt tattaaatca tatgaataca gagcaaagtg aagctgtaaa
     3301 gacaacagaa ggaccattgt taattatggc aggtgctggt tcagggaaga cacgtgtttt
     3361 aacacataga attgcttatt tattagacga aaaagatgtc tcaccataca atgttttggc
     3421 tattactttt acaaataaag ctgcaagaga aatgaaagaa cgtgttcaaa aattagtagg
     3481 tgatcaagca gaagttattt ggatgtcaac attccactca atgtgtgttc gtattttacg
     3541 tcgtgatgca gatcgaattg gtatagaacg caattttacg ataattgatc ctacagacca
     3601 aaaatctgtt attaaagacg tcttaaaaaa tgaaaatatt gatagtaaaa agtttgaacc
     3661 tcgtatgttt atcggtgcga tcagtaattt gaaaaatgaa cttaaaacac ctgcagatgc
     3721 tcaaaaagaa gccacagatt atcactcgca aatggtagca acggtttata gtggatatca
     3781 acgccaattg tcacgtaatg aagcgttaga ttttgatgac cttattatga caacgattaa
     3841 cttatttgag cgtgtaccag aagttctaga atattatcag aacaaattcc aatatattca
     3901 tgtagatgag tatcaagata ctaataaagc acaatacaca ttagttaaat tattagcaag
     3961 taagtttaaa aacttatgtg ttgtaggtga ctcagatcag tcaatttatg gttggcgtgg
     4021 tgctgatatt caaaatatct tatcatttga aaaagactat ccagaagcga atacaatctt
     4081 tttagagcaa aattatcgtt cgacgaaaac gattttaaat gcggctaacg aagtgattaa
     4141 aaataattct gaacgtaagc caaaaggact gtggactgca aatacgaatg gtgagaaaat
     4201 tcattactat gaagcaatga cggaacgtga tgaagcggaa tttgtaatac gagaaattat
     4261 gaagcatcaa cgtaatggta agaaatatca agatatggca attttatata gaacgaatgc
     4321 acaatcacgt gtacttgagg aaacattcat gaaatctaat atgccataca caatggttgg
     4381 tggccaaaag ttctatgacc gtaaagaaat caaagattta ttaagttatt tgcgtataat
     4441 tgccaatagt aatgatgaca ttagtttgca acgtattatt aatgttccaa aaagaggtgt
     4501 aggtccttca tctgttgaaa aagttcaaaa ctatgcactt caaaacaata tcagtatgtt
     4561 tgatgcactt ggagaagctg attttatcgg attgtcaaaa aaggtgacac aagagtgtct
     4621 taatttttac gagttaatcc aaagcttgat aaaagaacaa gaatttttag aaattcatga
     4681 aatagttgat gaagtattac aaaagtctgg ctatcgagaa atgcttgaac gtgaaaatac
     4741 attagaatct cgaagtagat tagaaaacat cgatgaattt atgtcagtac caaaagacta
     4801 tgaggaaaat accccattag aagaacagtc attaattaac tttttaacgg atttatcgtt
     4861 agtagctgat attgatgagg cagatactga aaatggcgta acactaatga cgatgcactc
     4921 ggctaagggc cttgaatttc caattgtctt tataatgggg atggaagaat ctttattccc
     4981 acatattaga gcgattaaga gtgaagatga tcatgaaatg caagaagaac gtcgtatttg
     5041 ttatgtagca attacaaggg ctgaagaggt gttatatatc actcatgcga catcaagaat
     5101 gttatttggt cgccctcagt caaatatgcc atccagattt ttaaaggaaa ttccagaatc
     5161 actattagaa aatcattcaa gtggcaaacg acaaacgata caacctaagg caaaaccttt
     5221 tgctaaacgc ggatttagtc aacgaacaac gtcaacgaaa aaacaagtat tgtcatctga
     5281 ttggaatgta ggtgacaaag tgatgcataa agcctgggga gaaggcatgg tgagtaatgt
     5341 aaacgagaaa aatggctcaa tcgaactaga tattatcttt aaatcacaag ggccaaaacg
     5401 tttgttagcg caatttgcac caattgaaaa aaaggaggat taagggatgg ctgatttatc
     5461 gtctcgtgtg aacgagttac atgatttatt aaatcaatac agttatgaat actatgtaga
     5521 ggataatcca tctgtaccag atagtgaata tgacaaatta cttcatgaac tgattaaaat
     5581 agaagaggag catcctgagt ataagactgt agattctcca acagttagag ttggcggtga
     5641 agcccaagcc tctttcaata aagtcaacca tgacacgcca atgttaagtt tagggaatgc
     5701 atttaatgag gatgatttga gaaaattcga ccaacgcata cgtgaacaaa ttggcaacgt
     5761 tgaatatatg tgcgaattaa aaattgatgg cttagcagta tcattgaaat atgttgatgg
     5821 atacttcgtt caaggtttaa cacgtggtga tggaacaaca ggtgaagata ttaccgaaaa
     5881 tttaaaaaca attcatgcga tacctttgaa aatgaaagaa ccattaaatg tagaagttcg
     5941 tggtgaagca tatatgccga gacgttcatt tttacgatta aatgaagaaa aagaaaaaaa
     6001 tgatgagcag ttatttgcaa atccaagaaa cgctgctgcg ggatcattaa gacagttaga
     6061 ttctaaatta acggcaaaac gaaagctaag cgtatttata tatagtgtca atgatttcac
     6121 tgatttcaat gcgcgttcgc aaagtgaagc attagatgag ttagataaat taggttttac
     6181 aacgaataaa aatagagcgc gtgtaaataa tatcgatggt gttttagagt atattgaaaa
     6241 atggacaagc caaagagagt cattacctta tgatattgat gggattgtta ttaaggttaa
     6301 tgatttagat caacaggatg agatgggatt cacacaaaaa tctcctagat gggccattgc
     6361 ttataaattt ccagctgagg aagtagtaac taaattatta gatattgaat taagtattgg
     6421 acgaacaggt gtagtcacac ctactgctat tttagaacca gtaaaagtag ctggtacaac
     6481 tgtatcaaga gcatctttgc acaatgagga tttaattcat gacagagata ttcgaattgg
     6541 tgatagtgtt gtagtgaaaa aagcaggtga catcatacct gaagttgtac gtagtattcc
     6601 agaacgtaga cctgaggatg ctgtcacata tcatatgcca acccattgtc caagttgtgg
     6661 acatgaatta gtacgtattg aaggcgaagt agcacttcgt tgcattaatc caaaatgcca
     6721 agcacaactt gttgaaggat tgattcactt tgtatcaaga caagccatga atattgatgg
     6781 tttaggcact aaaattattc aacagcttta tcaaagcgaa ttaattaaag atgttgctga
     6841 tattttctat ttaacagaag aagatttatt acctttagac agaatggggc agaaaaaagt
     6901 tgataattta ttagctgcca ttcaacaagc taaggacaac tctttagaaa atttattatt
     6961 tggtctaggt attaggcatt taggtgttaa agcgagccaa gtgttagcag aaaaatatga
     7021 aacgatagat cgattactaa cggtaactga agcggaatta gtagaaattc atgatatagg
     7081 tgataaagta gcacaatctg tagttactta tttagaaaat gaagatattc gtgctttaat
     7141 tcaaaaatta aaagataaac atgttaatat gatttataaa ggtatcaaaa catcagatat
     7201 tgaaggacat cctgaattta gtggtaaaac gatagtactg actggtaagc tacatcaaat
     7261 gacacgcaat gaagcatcta aatggcttgc atcacaaggt gctaaagtta caagtagcgt
     7321 tactaaaaat acagatgtcg ttattgctgg tgaagatgca ggttcaaaat taacaaaagc
     7381 acaaagttta ggtattgaaa tttggacaga gcaacaattt gtagataagc aaaatgaatt
     7441 aaatagttag aggggtatgt cgatgaagcg tacattagta ttattgatta cagctatctt
     7501 tatactcgct gcttgtggta accataagga tgaccaggct ggaaaagata atcaaaaaca
     7561 taacaatagt tcaaatcaag taaaagaaat tgcaacggat aaaaatgtac aaggtgataa
     7621 ctatcgtaca ttgttaccat ttaaagaaag ccaggcaaga ggacttttac aagataacat
     7681 ggcaaatagt tataatggcg gcgactttga agatggttta ttgaacttaa gtaaagaagt
     7741 atttccaaca gataaatatt tgtatcaaga tggtcaattt ttggacaaga aaacaattaa
     7801 tgcctattta aatcctaagt atacaaaacg tgaaatcgat aaaatgtctg aaaaagataa
     7861 aaaagacaag aaagcgaatg aaaatttagg acttaatcca tcacacgaag gtgaaacaga
     7921 tcctgaaaag attgcagaaa aatcaccagc ctatttatct aacattttag agcaagattt
     7981 ttatggtggt ggagatacaa aaggtaagaa tattaaaggt atgacgattg gtttagctat
     8041 gaatagtgtt tattactata aaaaagaaaa agatggatcg acttttagta aaaaactaga
     8101 tgatagcgaa gttaaaaagc aaggtaaaca aatggctagt gagatattat caaggttacg
     8161 tgaaaatgat gatttaaaag atataccaat tcattttgca atttataagc aatcaagtga
     8221 agattcaatc acaccaggtg aatttatcac tcaagcgact gcagaaaaga gtcaaacaaa
     8281 gcttaatgaa tggcataata tcaatgaaaa atcagcttta ttaccttctt caacagcagc
     8341 agattatgat gaaaatttaa ataataattt caagcaattt aatgataatt tgcaatcata
     8401 tttttctaat ttcacacaag cagtagggaa agttaaattt gttgataaaa agccacaacg
     8461 attagtagta gatttaccaa tcgattacta tggacaagct gaaacaattg gtattacaca
     8521 gtacgttact gaacaagcga ataaatattt cgataaaatc gataactatg aaattcgtat
     8581 taaagatggt aaccaaccac gtgctttaat tagtaagaca aaagatgaca aagaaccgca
     8641 agttcatatt tacagtaatt aattgtaaat taaagataat aaaaaggtac caatgttagt
     8701 cataattttg attaacatta gtaccttttt aatttgttaa gacttatgaa ttatttttct
     8761 ctaacgatgt cacgaacttt gtttaagtca gtttcaacaa atgcaccagg ttttttagta
     8821 agtttactta caacatatgt aacgattaca cttacaataa atccaggaat aatttcatat
     8881 aagccgaata tttcgttgat atgtgccaat ggtttaatcc acgcaatcca aacgataacg
     8941 actaaggcac ctgaaaccat tccacttaca gcaccggcac gtgtcaaacc tttccagtaa
     9001 agtgcaaata gcacaagtgg actgaacgat gcaccaaatc cggcccaagc gttacctact
     9061 aagtttagaa ttgtgtcgtt tggattccat gcaatcgcga tggcaacaat tgctacaact
     9121 aatacagata atcttccaat cataacaaat tctttttggt gcgttttagc tttttcttca
     9181 ccacgaatta atttataaaa gtcttccgtt agtgaactag atgttacaag taattgtgaa
     9241 gaaatcgtgc tcataattgc agctagaatc gcagcaagta agaaaccacc tacaagagga
     9301 tggaagagta cttgactcat cacgatgaat aatgtctcag gatcttctag tttaatatga
     9361 taagcaggta cgaatgcaat acctgttaaa ccaacagcca cagcgcctaa taaaccaaca
     9421 gccatccagc taatacctaa acgtctagct ttaggtagca tcttgtgtga tttaatagac
     9481 ataaacctta taatgatatg aggttgaccg aaataaccta atccccatga aaatagagag
     9541 ataattccta taaatgataa ccctttaaat aaatttaaat ttgtaggttt catagctgct
     9601 acatcatgaa acgttcccca gccgtttaaa ttcatcatag caacaatagg gaccataacc
     9661 atcgcaatta acataatgac accttggaag aaatctgtaa ttgatacagc taaatatcca
     9721 ccaaagaaag tatagaaaat gacaatgaaa gcaactaata ttaaaccgaa atgataatct
     9781 aatccaaaag cactttcaaa tagtttacca ccagatacga aaccagaatg tgtatataat
     9841 gtaaagaata ctacgataat caatccagaa ataatcttta acacattatt tttatcgttt
     9901 aaacgatttt taaagaaatc tggtaatgta attgcatctc cagctaattc ggtataaaca
     9961 cgaagtctag gagcaacaac aaagtaattt atataagcac ctaatgttaa accgattgta
    10021 atccacatag ctgatagacc agtgctatag acagaaccag gtagccccat aatcatccat
    10081 ccactcatat ctgaagctcc agctgataat gcagtaatat acggtccaat actacgtcca
    10141 cctaacatgt actcgcttag gttaccagtt gcttgcttgt aaccgtaaaa gccaataacg
    10201 attagtatca agaagtagac ggcaatcata atataagttt gccaattggc atctacttgt
    10261 tgacttaatg ctgtccccat tgtaagcatg ataaacactc cctttattta atttataatt
    10321 tagttacttg aaaacattat acataaagat aaatgtttag aaaag
//
LOCUS       DAKTLH010000089        10295 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.089, whole genome shotgun sequence.
ACCESSION   DAKTLH010000089 DAKTLH010000000
VERSION     DAKTLH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10295
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.089"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            67..993
                     /locus_tag="O0394_001845"
     CDS             67..993
                     /locus_tag="O0394_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0499918.1"
                     /translation="MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQ
                     KEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLT
                     AMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWF
                     PVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLF
                     GHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAIT
                     LLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
     gene            993..2063
                     /locus_tag="O0394_001846"
     CDS             993..2063
                     /locus_tag="O0394_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0499919.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVEHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            2080..3162
                     /locus_tag="O0394_001847"
     CDS             2080..3162
                     /locus_tag="O0394_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0499920.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            3152..4093
                     /locus_tag="O0394_001848"
     CDS             3152..4093
                     /locus_tag="O0394_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0499921.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFDIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQRERGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPAEEIYQNPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPNHFVFSTEEEAAQLRENKLVTQN"
     gene            4112..5767
                     /locus_tag="O0394_001849"
     CDS             4112..5767
                     /locus_tag="O0394_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0499922.1"
                     /translation="MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALN
                     DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            5979..7694
                     /locus_tag="O0394_001850"
     CDS             5979..7694
                     /locus_tag="O0394_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0499923.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPA
                     KKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKT
                     DHISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYP
                     IGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVAN
                     DATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRK
                     AMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLG
                     YKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFG
                     KYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDF
                     EKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYN
                     LTKEA"
     gene            7745..8731
                     /locus_tag="O0394_001851"
     CDS             7745..8731
                     /locus_tag="O0394_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0499924.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSNGEVIFDGKRIDTLNEKQLPDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANKLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEEQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKLDICNNQSPKMHVCEDVIVRCHLYKNEYKEI"
     gene            8734..9714
                     /locus_tag="O0394_001852"
     CDS             8734..9714
                     /locus_tag="O0394_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCY0499925.1"
                     /translation="MENILEVNQIKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFNLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKHNNNRIILKGELPS
                     PSNPPQGCPFHTRCPIAQDMCKKSMPELKDIGNEHQVACFYVDKVGDLND"
     gene            9707..>10295
                     /locus_tag="O0394_001853"
     CDS             9707..>10295
                     /locus_tag="O0394_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0499926.1"
                     /translation="MIKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPAFTLGL"
BASE COUNT         3824 a         1420 c         1927 g         3124 t
ORIGIN      
        1 agatttcaga aaattttgaa gggaatggaa atttagaaat cggaatttgt tagaggaggg
       61 gattagatgg ggaaatatat tttcaaacga tttatttata tgcttatttc tttatttatt
      121 attattacaa ttacattttt cttaatgaaa ttaatgccag gttcgccatt taacgatgct
      181 aaattaaatg ctgaacaaaa agaaatttta aatgaaaaat atggattaaa tgatcctgta
      241 gctacgcagt atttacatta tttaaaaaat gttgttacag gcgattttgg taattcattc
      301 cagtatcata atcaacctgt gtgggatttg attaaaccga gactactacc ttcttttgaa
      361 atgggtctta cagcaatgtt catcggtgtg atactgggac ttattttagg tgttgcagca
      421 gctactaaac aaaattcttg ggttgactat acaactacag ttatttcagt tattgcagta
      481 tctgtaccat cttttgtact tgctgtactt ttacaatatg tatttgcagt taaattaaga
      541 tggttcccag tagctggatg ggaaggtttt tcgaccgcgg tattaccgtc acttgcatta
      601 tctgcagctg ttttagcaac tgtcgccaga tacataagag cagagatgat agaggtatta
      661 agttcagact atattttatt agcgagagct aaaggtaatt cgacaatgcg tgtacttttt
      721 ggacatgcac ttagaaatgc tttaattcca attattacaa ttatcgttcc catgttagca
      781 agtattttaa caggcacttt aacaattgaa aatatttttg gagttcctgg attaggggat
      841 caattcgtac gttcaattac aacaaatgat ttctcagtaa tcatggcaat cacactatta
      901 tttagcacac tgtttatcgt ttctattttt attgtagata ttttgtacgg tgtgatagat
      961 ccacgaattc gtgttcaagg aggtaaaaaa taatggctga aaataaaaac aatttgtcga
     1021 ttaacgacga tcattctaat gcagctatga cgcatacctc tgacgctatc gcatcatctg
     1081 attttattat tagagaatta gatttgaatc aggaacctga aatgcaacga gaaagcaaaa
     1141 acttttggca agatgcttgg gctcagttaa aacgaaataa gttagctgtt gtcggtatga
     1201 taggtttaat tatcattgta atatttgctt ttatcggtcc agttataaat aaacatgatt
     1261 atgctgaaca aaatgtagaa catagaaatc ttccggcaaa aatacctgta ttagacaaag
     1321 ttccattttt accttttgat ggtaaagatg cagatggcaa ggatgcttat aaagcagcaa
     1381 atgctaaaga aaattattgg tttggtactg atcagttggg tcgagattta tggacaagaa
     1441 catggaaagg tgctcaaatt tcattgttta tcggtgttgt tgcagcgatg ttagatattt
     1501 ttattggtgt tgtatatggt gcgatttctg gattcttcgg tggacgtgtc gatacgatta
     1561 tgcaacgtat acttgaagtc atagcatcta ttccgaattt aattgtcgta attttatttg
     1621 tattaatttt tgaaccatcc atttggacaa ttatattggc tatgtctatc acaggctggt
     1681 taggcatgag cagagttgta cgtggagaat ttttaaaatt aaaaaatcaa gagtttgtca
     1741 tggcttcgaa aacattgggg gcttcaaaat tcaaattgat atttaagcat attttaccta
     1801 atacattagg tgctatcgtg gttacatcaa tgtttacagt acctagtgct attttcttcg
     1861 aagcattttt aagtttcatt ggtataggtg tacccgcacc tcaaacatcg ttagggtcat
     1921 tagtaaatga tgggcgcgca atgttattaa tttatccaca tgaattattt ataccagcaa
     1981 tgattttaag tttattaatt ctattctttt acttatttag tgatggatta cgtgatgcat
     2041 ttgatccgaa aatgcgtaaa taaaaagggg gcatagcata tgactgaaag aatattagaa
     2101 gtaaatgatt tgcatgtttc ctttgatatt acagcagggg aagtgcaggc agtgagaggc
     2161 gtagattttt atttgaacaa aggggaaaca ttggcaattg ttggtgaatc aggttcaggt
     2221 aaatctgtaa caacaaaagc aattacaaaa ttattccaag gggacacagg aagaattaaa
     2281 aagggagaaa ttttattttt aggggaagat ttagcaaaaa aacctgaaaa tgagttgatt
     2341 aaattacgtg gcaaagatat ttcaatgatc tttcaagatc caatgacatc tttaaaccca
     2401 acgatgcaaa ttggtaaaca agtcatggaa ccattaatta agcacaaaaa ttatagtaaa
     2461 gcacaagcta aaaagcgcgc attggaaata ctaaatcttg taggtttacc aaatgcagaa
     2521 aaaagattta aagcatatcc tcatcaattt tcaggtggac aaaggcaaag aattgttatt
     2581 gcaaccgcat tagcttgtga acctaaagtg ctcattgctg atgaaccaac gactgcatta
     2641 gacgtaacga tgcaggcaca aattttagat ttaatgaaag aactacaaca aaaaatcgat
     2701 acagcaatta tttttataac gcatgattta ggggttgttg cgaatattgc tgatagagtg
     2761 gcagttatgt atggtggtca aatggttgaa acaggagatg ttaacgaaat attttatgat
     2821 ccaaagcatc catatacatg gggattatta tcgtcaatgc ctgatttatc aacaacaaat
     2881 gacacaccat tactagcgat tcctggagcg ccacctgatt tattacaccc acctaaaggt
     2941 gatgcatttg cgagacgtag tcaatatgca ttagatattg attttaaagt agaaccaccg
     3001 tggtttaaag tttcaccgac acattttgtg aaatcttggt tattagacgc acgtgcacca
     3061 aaagttgaac tacccgagct ggtaaaacaa cgtatgaaac cgatgcctaa taattatgaa
     3121 aaaccactca aggtagaaag ggtgtcgttc aatgaaaaat gatgaagtgc tattatctat
     3181 taaaaattta aagcaatatt ttaacgcagg aaagaaaaac gaagtgagag cgattgaaaa
     3241 tatttcgttt gatatataca aaggggaaac attaggttta gtaggagaat cggggtgtgg
     3301 taaatctaca actggtaaat caattattaa acttaatgat attacaagtg gagaaatttt
     3361 gtatgagggt attgatatac aaaagattcg taaacgtaaa gatttgctta aatttaataa
     3421 aaagatacag atgatttttc aagacccata tgcgtcttta aatcctaggt taaaagtaat
     3481 ggatatagta gctgaaggta ttgatatcca tcatttagca actgataagc gtgaccgaaa
     3541 aaaacgtgtc tatgatttac ttgaaactgt tggattaagt aaagaacatg ccaatcgcta
     3601 tcctcatgaa ttttcaggtg gacaacgcca acgtattgga attgcccgtg cattagccgt
     3661 tgaaccagaa ttcattatcg cggacgaacc aatatcggca ttggatgttt caatccaagc
     3721 tcaagtagtt aatttattat taaaattaca acgtgaaaga gggattacgt tcctatttat
     3781 agctcatgat ctatcaatgg tgaagtatat ttcagatcgt attgcagtca tgcattttgg
     3841 gaaaatagtt gaaattggac cggcagaaga aatttatcaa aatccattac acgattatac
     3901 taagtcttta ttatcagcca ttccacaacc tgatcctgaa tcagaacgca gtcgcaaacg
     3961 atttagttat attgatgatg aagcaaataa tcatttaaga caattacatg aaattagacc
     4021 gaatcacttt gtctttagta ctgaagaaga agcggcacaa ctacgagaaa ataaattggt
     4081 gacacaaaat taaggggaag ggggaaatgc aatgacgaga aaatttagaa cacttatttt
     4141 aattttgatt gctacaattg cattaagtgg ttgtgctaat gacgatggta tttattcaga
     4201 taaaggtcaa gtattcagaa aaattttgtc atcagactta acatcccttg atacatcatt
     4261 aataacggat gaaatatctt ctgaagtgac tgcgcaaaca ttcgaaggtt tatacacatt
     4321 aggaaaaggt gacaaaccgg tgttaggtgt tgcgaaagct tttcctgaaa agagtaaaga
     4381 tggtaaaact ttaaaggtta aattaagaag cgatgctaaa tggagcaatg gtgacaaagt
     4441 gactgcacaa gactttgttt atgcttggag aaaaacagtt gaccctaaaa caggttctga
     4501 atttgcatac attatggggg acattaaaaa tgcgagtgat attagtactg gtaagaaacc
     4561 tgtagagcaa ttaggtatca aagcattaaa tgatgaaaca ttacaaattg aattagaaaa
     4621 gccggttcca tatattaatc aattattagc acttaatacc tttgcacctc aaaatgaaaa
     4681 agttgccaaa aaatatggta aaaattacgg tacggcagct gatagagcgg tatacaatgg
     4741 tccatttaaa gttgatgatt ggaaacaaga agataaaacc ttactatcta aaaatcagta
     4801 ttattgggat aaaaagaatg taaaattaga taaagtgaat tataaagtta ttaaagactt
     4861 acaagccggt gcatcattgt atgatactga atcagtagat gacgcagtta ttactgcaga
     4921 tcaagtaaat aaatataaag acaacaaagg attaaacttt gtgttaacga ctgggacatt
     4981 ttttgtaaaa atgaatgaaa aacaatatcc tgattttaaa aacaaaaatt taagattggc
     5041 tatcgcacaa gcaatagata aaaaaggata cgttgattca gtgaaaaaca atggctcaat
     5101 tccttccgat acactaacag ccaaaggaat tgcgaaagcg cctaatggca aagattatgc
     5161 gagtaccatg aattcgcctt taaaatataa tcctaaagaa gcaagagcgc actgggacaa
     5221 agctaaaaaa gagttaggta aaaatgaagt gacattttca atgaacacag aagatacacc
     5281 agatgcaaaa atatctgctg aatatatcaa atcgcaagtt gagaaaaatt taccaggagt
     5341 tactttgaaa attaagcaat taccgtttaa acaaagagta tcactagaac tgagtaacaa
     5401 ttttgaagca tcacttagtg gttggtctgc agattaccct gatcctatgg cttatttaga
     5461 aacaatgacc acaggtagcg cacaaaataa tacagactgg ggtaataaag aatatgatca
     5521 attacttaaa gtagcaagaa ccaaattggc acttcaaccg aacgaacgat atgaaaactt
     5581 gaaaaaagca gaagaaatgt tcctaggaga tgcaccggta gcaccaattt atcaaaaagg
     5641 tgttgcacat ttaacaaatc ctcaagtaaa aggattaatt taccataaat ttggtccaaa
     5701 taactcactt aaacatgtat atattgataa atcgatagat aaagaaacag gtaagaagaa
     5761 aaaataatat gctttgtaaa ttaggctgga gacatatctc cagtcttttt gtgttggata
     5821 aaaactttgg gaataaaaat ttaaaataag tcgtttttta aattactgaa attgattaaa
     5881 tgcataaata actgaatatt ctaaaaataa acttgtaata attttttcta tgagtaaact
     5941 aaaaagaaaa aattagattg aaagtaggag gcatatgtat ggggaagcta attaaatata
     6001 tttcaatact tcttattgtc gttttagtgt tgagtgcttg cggaaaaagc agtaataaag
     6061 atgaaggagt aaaagatgct actaaaacgg aaacctcaaa acataaaggt ggtaccttaa
     6121 atgtagcatt aacagcaccg ccaagtggtg tttattcttc gttattaaat agtacacatg
     6181 cagattctgt agttgaggga tattttaacg aaagcttatt agcaactgat aaaaaaatac
     6241 gtcctaaggc atatattgct tcatggaagg acatcgagcc ggctaagaaa atagaattta
     6301 aaattaaaaa aggtattaaa tggcatgatg gtaatgaatt gaaaattgat gattggattt
     6361 attcaattga agtcttagct aacaaggact acgaaggtgc ttattatcca agtgtagaaa
     6421 atatccaagg tgcgaaagat tatcatgaag gaaaaactga tcatattagc ggattgaaga
     6481 aaatagatga ctacactatg caggttacat ttgataaaaa acaagaaaat tacttaacag
     6541 gatttattac tggaccttta ttaagtaaaa aatatttatc agatgtacca attaaagatt
     6601 tagcgaaatc agataaaatc cgaaaatatc ctattggtat tggaccgtat aaagttaaga
     6661 aaatcgttcc aggtgaggct gttcaactcg ttaaatttga tgattattgg caaggtaagc
     6721 ctgcactaga caaaatcaat ttaaaagtta ttgatcaagc gcaaattatt aaggcaatgg
     6781 aaaaaggcga tattgatgtt gcgaatgatg ctaccggtgc aatggcaaaa gatgctaagt
     6841 catctaatgc tggtctcaag gtattatctg cgccaagctt agactacggt ttaataggat
     6901 tcgtatctca tgattacgat aaaaaagcta ataaaactgg taaagtgaga ccaaaatatg
     6961 aagacaaaga attacgtaaa gcaatgcttt atgcaattga tagagaaaaa tggatcaaag
     7021 cgtttttcaa tggttacgct agtgaaatca atagttttgt accatctatg cattggatag
     7081 cagccaatcc taaggaccta aatgattaca aatatgatcc tgaaaaagct aaaaaaatct
     7141 tagataagtt aggttataaa gatagagatg gtgacggatt tagagaagat cctaaaggta
     7201 ataaatttga gattaacttt aaacattatt caggttctaa tcctactttt gaaccaagaa
     7261 ctgctgcgat aaaagatttc tgggaaaaag ttggcttgaa aacaaatgtg aagttagtag
     7321 aattcggtaa atataatgaa gacttagcaa atgcatctaa agatatggaa gtgtacttca
     7381 gatcatgggc aggaggtaca gatccagatc catcagattt ataccacact gatagacctc
     7441 aaaatgaaat gagaacagtt ttaccaaaat cagatcaata tttagatgat gcattagact
     7501 tcgaaaaagt aggcattgat gaaaagaaac gtaaagatat ttatgttaaa tggcaaaaat
     7561 atatgaatga tgagttacct ggattaccaa tgttccaagg taaatcgata actattgtta
     7621 acgataaagt acgaaactta gacattgaaa ttggaactga tcaaagttta tataatttaa
     7681 ctaaagaagc ttagtaatgt aacctcgacg attttgtcga ggtttactta tatgggggga
     7741 tgttatgaat aatgtattgt tagaggttaa agatttagaa acatcattaa aaataaataa
     7801 tgaatggtta gcaactgttg aaaatatttc ttttgaatta tctaaaggag aagttttggg
     7861 tatagtaggg gaatctggtt gcggtaagtc catattaagt aagtcaatta ttaaattatt
     7921 accagaaaag atatctaaac taagtaatgg agaagttata tttgatggta aacgaatcga
     7981 tacgctcaat gagaagcaat tgccagatat tcgaggaaat gatattgcta tgatttttca
     8041 agaacctatg actgctttaa atcctgtatt taccataaaa aatcaacttg tggaatctat
     8101 aaaatcacat aaaaaaattt ctaaaaaaga agcaaataaa ttagcaaaag atttactaaa
     8161 aaaagttgga attgctagac aagatgaaat attaaatagc tatcctcatc aattatctgg
     8221 tggtatgaga caaagagtaa tgattgcaat ggccatttca tgttctccta aattattaat
     8281 tgctgatgaa cctacaacag cattggatgt cacgattcaa gcgcaaatat tagacttatt
     8341 aaaagaattg caaaaggaaa cgcaaatggc aattatgatg attacacatg atttgagtgt
     8401 agttgctgag ttttgcgata aagtcttagt tatgtatgca ggtcaaattg tagaatttgg
     8461 aggcataaaa gaaatactac acaatccgaa acatccttat acccaaaaat tattatcaac
     8521 aattccaaaa cttaaagaag agcagaaacg acttgaaacg atagaaggaa ttgtgccatc
     8581 aatccaagca tttcacgtta ataagtgcag atttgcaaat agatgtaaca aaaaactgga
     8641 tatttgtaat aatcaatctc ctaaaatgca tgtttgtgaa gatgtcattg tacgttgtca
     8701 tttgtacaaa aatgaatata aggagatata agaatggaaa atattttaga agtcaaccaa
     8761 ataaaaaaat actacaaaat taaaactgga ttattacaaa aaactcagta cgttaaagct
     8821 gttgatgacg tatcgttttc aataaaaaaa ggacaaactt ttggattagt aggagaatcg
     8881 ggttgtggta agtcaacgtt aggtaaagtg attatcaggc ttgaagatgc aacttcaggc
     8941 tcaataattg ttaatggtga agatataaca agattacaag gtaaaaaact cagaaaatca
     9001 cgacaacaat atcagatgat atttcaagat ccgtatgcat cattgaatcc gatgcaaatg
     9061 gttggagata tcatttcaga acctatttta aattataaaa aattgccaaa agaagaaata
     9121 aaaaaagaag tactatattt attaaaatgt gttggcctaa gtgaagatgc atattataaa
     9181 tatgcacatg aattttcagg tggacagaga caaagagtgg gaattgcaag agcattggct
     9241 ttgcgtccga gtttaattgt tgctgatgag cctgtaagtg cattagatgt atctgttcaa
     9301 tctcaagtac tgaatttatt aaaagattta caagaacaat ttaacttaag ctatttattt
     9361 atcgcacatg atttaagtgt agtaaaacat ataagtgatg tcattggagt tatgtattta
     9421 ggtcatatag ttgaaatcgc atctgataaa gaaatttatg aaaatcccaa acatccatat
     9481 acaaaagcgt tgatttcatc aataccacaa attgataaac ataataacaa tagaattata
     9541 ttaaaaggag aattaccttc gccaagtaat ccgccgcaag gttgtccttt tcatacaaga
     9601 tgtccaattg ctcaagatat gtgcaaaaaa agtatgccag aattaaagga cattggtaat
     9661 gaacatcaag ttgcttgttt ctacgtagat aaagtaggtg atttaaatga ttaaattaat
     9721 acttaaaaga ttagggttaa tgatcccgtt actaatttta atctcaatag ttgtattttc
     9781 attagcaatc atacaaccag gagatccatt ttcagatcta caaaacggta aaataaaaca
     9841 agaagcaata aatgcacaaa gggaaaaatt aggtctcaac gactcaatac cacatcaata
     9901 cattagatgg gtcaatcatg ttatacacgg tgatttaggt gagtcaatca aatataaaag
     9961 gccggtaatt gatgttattg aggaaagaat tccaaataca atattactcg gtgctatgtc
    10021 attaattatt acttatatta tctcatttgc tttaggaata acgtcaggta gatattctta
    10081 cagtttgacg gattatactg tgcaaatatt taattatttg atgttagcca ttccatcttt
    10141 tattgcggga gtatttgcaa tttttatttt ttcttttgaa ttacaatggt tcccgtttca
    10201 aggttctgtt gatattaacc ttaaagaagg tacttttgaa tattatatga gtaaaatata
    10261 tcacacattt ttacctgcat tcactttagg tttat
//
LOCUS       DAKTLH010000090        10093 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.090, whole genome shotgun sequence.
ACCESSION   DAKTLH010000090 DAKTLH010000000
VERSION     DAKTLH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10093
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.090"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            1195..2064
                     /locus_tag="O0394_001854"
     CDS             1195..2064
                     /locus_tag="O0394_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCY0499927.1"
                     /translation="MEMSRLNLTDDNDIDEIIAWRGEQEELEQKRDTYKKRYHEFEME
                     IARLESLTKDKELLDSDKLKDEYELKKGKMNTLIDEYSAVHYQCQNNINKTQSIVSHI
                     NYLNQELKDQQEIFQLAEIVSGKNNKNLTLENFVLIYYLDQIIAQANLRLATMSDNRY
                     QLIRREAVSHGLSGLEIDVFDLHSNKSRHISSLSGGETFQSSLALALGLSEIVQQQSG
                     GISLESIFIDEGFGTLDQETLETALDTLLNLKSTGRMVGIISHVSELKNRIPLVLEVK
                     SDQYQSSTRFKRN"
     gene            complement(2135..2497)
                     /gene="mscL"
                     /locus_tag="O0394_001855"
     CDS             complement(2135..2497)
                     /gene="mscL"
                     /locus_tag="O0394_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCY0499928.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
     gene            2701..4347
                     /locus_tag="O0394_001856"
     CDS             2701..4347
                     /locus_tag="O0394_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCY0499929.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
     gene            5239..7944
                     /gene="acnA"
                     /locus_tag="O0394_001857"
     CDS             5239..7944
                     /gene="acnA"
                     /locus_tag="O0394_001857"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCY0499930.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            8124..8591
                     /locus_tag="O0394_001858"
     CDS             8124..8591
                     /locus_tag="O0394_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCY0499931.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDGIDSL"
     gene            complement(8798..9094)
                     /locus_tag="O0394_001859"
     CDS             complement(8798..9094)
                     /locus_tag="O0394_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HCY0499932.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(9470..10078)
                     /gene="plsY"
                     /locus_tag="O0394_001860"
     CDS             complement(9470..10078)
                     /gene="plsY"
                     /locus_tag="O0394_001860"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCY0499933.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLQVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
BASE COUNT         3590 a         1476 c         1820 g         3207 t
ORIGIN      
        1 agagaaaaaa ttgaaactaa aattgctaat agcaaaaaag atatttctaa aaccaataat
       61 aaaatattga atcttgattg tgataagcga aacatagaca aagagaaaaa aatgttagaa
      121 gaaaatggag atttaattga aagtaaaatc tcttttattg ataaaactag agtattattt
      181 aacgatatta ataagtatca acaaagttat ctcaatattg aacgcttgag aactgagggt
      241 gaacaattag gtgatgaatt aaatgatcta attaaaggtt tagaaacggt cgaagattca
      301 ataggtaata accaaagtga ttacgagaaa attatcgaac taaataatac gataacgaac
      361 ataaataatg aaattaatat aattaaagaa aatgaaaaag ctaaagctga attagataaa
      421 ctattaggta gtaagcaaga gttagagaat caaattaatg aagaaacatc tatattgaag
      481 aatctcgaaa taaaattaga tcgctacgat aaaacaaaat tggacttaaa tgataaagaa
      541 agctttataa gtgaaattaa atctgctgta aatattggag atcaatgtcc gatatgtggt
      601 aatgaaattc aggatttagg gcatcatatt gattttgaca gtattgctaa acgtcaaaat
      661 gaaattaaag aaattgaagc aaatatccac gctataaaat cgaatattgc tgtgcataat
      721 tctgaaatta aatttgttaa tgaaaaaata tcgaatatta atattaaaac gcaaagtgat
      781 ttttcacttg aagtattgaa taagcgtctg ctagaaaatg aaaatgcatt gaataatcaa
      841 agagatctta ataaatttat agaacaaatg aaagaagaaa aagataatct aacgttgcaa
      901 attcataata aacaattgcg tctaaataaa aatgaatctg agttgaaatt atgtcgagat
      961 ctcatcactg aatttgaaac actctcgaaa tataataata tcactaattt tgaggtggat
     1021 tataagaagt atgttcaaga tgtgaatcag catcaagaac tctcaaagga gattgaagat
     1081 aagttaatgc aattgtctca aagaaagtta attgagcaaa ataatctaaa tcattatgaa
     1141 aatcaactag aaacttacaa taatgactta gaattgaatg aacagtctat tgaaatggaa
     1201 atgtcgaggc tgaatttaac tgatgacaat gatatagatg aaataatagc ctggaggggc
     1261 gagcaagagg aattagagca gaaaagggat acttataaaa aacgttatca tgaatttgaa
     1321 atggaaatag ctaggttaga atcattaact aaggataaag agttattgga ctctgataaa
     1381 ttaaaagatg aatatgagct aaaaaaagga aagatgaata cactgataga tgaatactct
     1441 gctgttcatt atcaatgtca aaataatatt aataaaacac aatctatagt ttcgcatatt
     1501 aattacttaa atcaagaatt aaaggatcaa caagaaatat ttcaattggc tgaaattgtc
     1561 agtggtaaga ataacaaaaa tcttacattg gaaaactttg tcttaattta ctatttagat
     1621 caaattattg ctcaagcaaa tctgagatta gcaacaatgt cagataatcg ataccaacta
     1681 attaggcgag aagcggtttc tcatggtctt agtggcctag aaattgatgt atttgatttg
     1741 cattcaaata agtctagaca tattagctcg ttatcaggtg gagaaacttt ccaatcgtcg
     1801 cttgcattag ctttagggtt aagcgaaatt gtacagcagc aatcaggagg tatttcacta
     1861 gaatcaatat ttattgatga aggattcggt acattagatc aagaaacgct tgaaacagcg
     1921 ttagacactt tattaaatct taaatcaact ggtagaatgg ttgggattat ttcacatgtg
     1981 agcgaattga aaaatagaat acctttagtt ttagaagtga aatcagatca atatcagagt
     2041 tcaacaagat tcaaaagaaa ttaaaacata aaaaatagag atggtacttt ggaatgtata
     2101 gaacaacctt agcccatctc tttttattta aaaattattt tttctcacgt aataaatctc
     2161 tgatttcagt taataacaca acattttctt ccacaactgc ttcttcttcg gcttcttctt
     2221 tcttcattaa tgtatttgca atcttaacaa agatgaataa agcaaacgcg atgataataa
     2281 agtcgataac agattggata aataaaccgt atttaatacc ccagaatgac cattctttag
     2341 caaaatcaac tgatccgaaa attttaccaa ttaatggcat aatgatattt tctactaatg
     2401 aagatataat cttgttgaaa gctgcaccca tcacaacagc aattgctaaa tctaagacgt
     2461 taccttttaa ggcgaactct ttgaattctt ttaacattac actcaacctc tcttttttaa
     2521 atgtaacctc aattatacaa caaataaaaa aattagaaaa tagattattc aagaaaattt
     2581 gattatcgca aagacctttg ttatcctata aaggtgcgtg agttattgaa taaaatgttt
     2641 agataatatt tttattctac tacataaaat taaatataga attgaaggga gtaattaatt
     2701 atgaattctt cttcaccaga gaatccaaat ggaaagaagt attcaccagt cttcatctat
     2761 agtgcaattg ttgttgctat agtcgtatta cttggtgcat ttttacctga acaattcaac
     2821 tatgttacca ataatattaa aatgtggatt acagaaaagt taggttggta ttatcttatt
     2881 cttactacga ttatcgtgtt cttctgtata ttccttattt ttagtcctat tggaaaactt
     2941 aaactaggta aaccaaatga caaacctgag tttaatacaa tttcatggtt tgctatgttg
     3001 tttagtgctg gtatggggat aggtttggtg ttttatggtg cagctgaacc gatggcgcac
     3061 tttgctacgc cacctacagc agatcccaaa actactgaag cttatactga agctctacgt
     3121 tcaacatttt tccattgggg attccatgct tgggctgttt atggtgttgt tgcgttagcg
     3181 ttggcatatt cgcaattccg taaaggtgaa ccaggtttat tatctagaac tttacgtcct
     3241 cttttaggtg ataaagtaga aggtcctatt gggattttta ttgacgtttt atctgtattt
     3301 gcgacaatcg ttggggtagc cgtttcgtta ggtatgggtg ctctacaaat taatggtggt
     3361 ttacattact tgttcaatgt tccaaacaat acgtttgtac aagcgattat catcattgtt
     3421 gttactatct tatttatagc aagtgcatgg tctggattaa gtaaaggtat tcaatactta
     3481 agtaacttga acattggttt aggtactatt ttaatggtag ctgctttaat tgttggacca
     3541 actgttctta ttttaaatat gttaactagc tctacgggta gtttactaaa cacattcttg
     3601 tttaatagtt ttgatacagc agctttaaat cctcaaaaac gtgaatggat gtcttcatgg
     3661 acactttatt actggggttg gtggttaagt tggagtccat tcgttggagt gtttattgca
     3721 cgagtttcaa aaggacgttc aattagagag ttcatttctg gtgtcttgct agttccagca
     3781 attgttagtt ttgtttggtt tagtgtcttt ggtgtattag gcatcgagac aggtaagaaa
     3841 cacaaagaaa tttttgatat gactcctgaa acacagctat ttggagtgtt taatcatgtg
     3901 ccatttggca ttgttttatc gttgattgca ttattattaa ttgcatcatt ctttattaca
     3961 tctgctgact cagcaacatt tgtattagga atgcaaacaa catttggttc attaaatcca
     4021 tctagtatgg taaaagttgt ttggggaatt tcacaggcct taatagcatt tgtactttta
     4081 ttagctggtg gcggtaacgg cgctgaagct ttaaatgcga ttcaaagtgc tgcaattata
     4141 agtgcattcc cattctcctt tgtcgtcata ctcatgatgg taagtttcta caaggatgcg
     4201 aaccaggaac gtaaattcct aggtttaaca ttgactccga ataaacatcg cttacaagaa
     4261 tatatcaaga gtcaacaaga agattatgaa tctgacattc ttgaaaagcg tcagtcacgt
     4321 agaaatatag agaaaaaaga taactaataa agtttagtta agtattttaa taacaagtag
     4381 tatgtcattc tagtagctag aacagatata ctacttgttt gtttttgtgg aaaattgagt
     4441 atattcaaaa ggataagaac gagaatttcg ttattaaact acgaattctc gattttttta
     4501 tattttaaga taggtttatt tctgaaaact taatagaaag gggtttgaca aagctaaagt
     4561 gaagtttgac ggtataaacg caaattaaat atacttttat agaaaattaa ctcaggctgg
     4621 gacataaatc aatattctat gctctacgaa gttatattgg cagtagttga ctgaacgaaa
     4681 atgcgcttgt aacaagcttt tttcaattct agtcaggggc cccaacacag agaatttcga
     4741 aaagaaattc tacaggcaat gcgagttggg gtgagggccc caacacagaa gctgacgaaa
     4801 agtcagctta caataatgtg caagttgggg atgggcccca acaaagagaa attggattcc
     4861 caatttctac agacaatgca agttggggtg ggacgacgaa ataaattttg cgaaaatatc
     4921 atttctgtcc cactcccatc aaaagaatga cattgaaaaa aatattaaag tttggtgcca
     4981 taattttctt tctgctcaat gaaataatac gtttaaaaat tgaattgaat cattcgcgaa
     5041 attgataatt attctcaatt aataaaaaat acttattaca ccttacctgt atgataagtt
     5101 ttgcttatat actctgatta aaaagtcaaa acctaaatta agcgttttct aagcatgatg
     5161 agttgtttat caataaatat aataatacaa tttaatcaag gcatataaat ataaaaatgt
     5221 atcaaggggg atcattaaat ggctgcaaat tttaaagagc aatcaaaaaa acattttgac
     5281 ttgaatggcc aaagttatac ttactatgat ttaaaagctg tagaagagca aggtattact
     5341 aaagtttcca atttacctta ttcaattcgt gttttgttag aatctttact tcgtcaagaa
     5401 gatgattttg taattacaga cgatcatatt aaagctttaa gtcagtttgg aaaagatgga
     5461 aatgaaggcg aggtgccatt taaaccttct cgtgttattt tacaagattt cacaggtgta
     5521 ccagccgtag ttgatttagc ttctttacgt aaagcaatgg atgacgttgg gggagatatt
     5581 actaaaatta atccagaagt accggtggat ttagttattg accactcagt tcaagtggat
     5641 agctatgcaa atccagaagc tcttgaacgt aatatgaaat tagaatttga acgtaactat
     5701 gaacgttatc agtttttaaa ttgggcaacg aaagcatttg ataattacaa tgcagttcct
     5761 cctgcaactg gaatagttca ccaagttaac ttagaatatt tagcaagtgt tgtacatgtt
     5821 cgtgatgtag atggtgaaaa aactgcattt ccagatacat tagttggtac tgattcacat
     5881 acaacaatga taaatggtat tggcgtacta ggatggggtg ttggtggtat tgaagctgaa
     5941 gctggaatgc ttggacaacc ttcttatttc ccaattccag aggttattgg tgtacgacta
     6001 gtaaattcat taccacaagg cgcaacagca actgatttag cgttaagagt aactcaagag
     6061 ctacgtaaaa aaggtgttgt tggtaaattt gtggagttct ttggtccagg tgtacaacat
     6121 ttaccactag cagaccgtgc tacaattgca aacatggcac cagagtatgg agcaacttgc
     6181 ggattcttcc cagttgatga tgaatctctt aaatatatga agttaactgg tagatcagac
     6241 gaacatatcg cgctagtaaa agaatatttg aaacaaaacc atatgttctt tgatgttgag
     6301 aaagaagatc ctaattatac agatgttatc gaattggatt tatcaacagt tgaagcatcg
     6361 ctttcaggac caaaacgtcc tcaagattta attttcttaa gtgatatgaa atcatcattt
     6421 gaaaattctg taacagctcc agcaggcaac caaggacacg gtttagataa aagtgaattt
     6481 gataagaaag ctgaaattaa ctttaaagat ggatcaaaag ctacaatgaa aacaggtgat
     6541 attgcaatag cagcaattac atcatgtaca aatacatcta acccttatgt aatgttaggt
     6601 gcaggtttag ttgctaaaaa agcagttgaa aaaggcttga aagttcctga atacgttaaa
     6661 acttctctag caccaggatc aaaagttgtt accggatatt taagagatgc tggcttacaa
     6721 ccttatttag atgatttagg cttcaacttg gttggttatg gatgtacaac ttgtatcggt
     6781 aattcaggtc ctttattacc agaaattgaa aaagcgattg ctgatgagga cctattagtg
     6841 acatctgtat tatctggtaa ccgtaacttt gaaggtcgta tccatcctct tgttaaagcc
     6901 aattacctag cttcaccaca gttagttgtt gcttatgcat tagctggaac ggttgatatt
     6961 gatttacaaa atgaacctat tggtaaaggt aatgacggtg aagatgtata tttgaaagat
     7021 atttggccat caattaaaga agtttcagat accgttgata gtgttgtaac acctgaatta
     7081 tttattgaag aatataataa cgtatacaat aacaacgaat tatggaatga gattgatgta
     7141 actgatcaac ctctatatga ctttgatcct aattcaacat acattcaaaa tccatcattc
     7201 ttccaaggat tatctaaaga accgggtacg attgttccat taaatggttt acgtgttatg
     7261 ggtaaattcg gtgattctgt gacaactgac cacatctctc cagcaggtgc aattggtaaa
     7321 gatacgccag ctggtaaata tttacaagat catcaagtgc ctattcgtga atttaattca
     7381 tatggttcaa gacgtggtaa tcacgaagta atggttcgag gtacgtttgc taatatacgt
     7441 attaaaaacc aattagcgcc aggtactgaa ggtggtttta caacttattg gccaacaaat
     7501 gaagtaatgc ctatctttga tgctgcaatg aaatataaag aagatggtac aggtttagtt
     7561 gtattagctg gtaacgatta tggtatgggt tcatctcgtg actgggcagc aaaaggtaca
     7621 aacttattag gtgttaaaac agttattgca caaagttatg aacgtatcca tcgttcaaat
     7681 ttagttatga tgggtgtatt accattagag tttaaaaaag gtgaatcagc tgattctctt
     7741 ggtctagatg gtacagaaga aatttctgtt aatattgatg aaaatgttca accacatgac
     7801 tacgtcaaag ttactgctaa gaagcaagat ggtgatttgg tagaatttga cgctatggtt
     7861 cgttttgact cacttgttga aatggattac tatcgtcacg gtggaatttt acaaatggtt
     7921 ttaagaaata aattagcgca ataaaaaata gatatcacag taaaatttta atcggtattt
     7981 gaggcacttt gatagtgctc aaaaatactt ctataaactt tatgatataa taggtcggtt
     8041 caaaaccttt gtgttcttgg ccggcctctt cttgtatttt atgttagaat gagatataaa
     8101 gttaaactga aagaaggctt aaaatgatat atagtattac agaaatagaa gcgcgttatg
     8161 ctgaaactga taagatgggt gtaatttatc acgggaatta tgcaacttgg tttgaagttg
     8221 cgcggttgga ttatatatcg aagttaggtt ttagttatgc tgatatggaa aaacaaggaa
     8281 tcatttcacc tgtgactgac ctcaatgtca attataaaaa gtctattttt tatccagaaa
     8341 aagttaaagt taaaacttgg gttgaaaaat attcgagatt acgttcagtg tataaatatg
     8401 agatttttaa tgaaaaaggt gaacttgcaa ctacaggttc cacagaattg atttgcatta
     8461 aagaagatac ttttaagcct atacggttgg atcgttattt cccagattgg catgaagctt
     8521 atagtaaagt gcaaacgctc aataatgaag ggaaaacagt agagataatg gatggtattg
     8581 attctttata agttactgta aatgtttgga gaggcaatat taacaattaa tctataaaaa
     8641 atagccaata cgttaacatt aatgtaatta gcgtgaagat aagctaatat atagcaaaaa
     8701 cgaacttgtt catacaacat cagatgtgct aaaccaaagt acaacttccg aggttcagga
     8761 cacatcagaa tgtgtaaaca agttcgtttt attttagtta ttttgtggaa taagaaattt
     8821 catcttcgtg atcaactaca tttacaataa tgtggtcatc ttcaaagtac cacaaatctt
     8881 tttccgctac gacaacattt aaatcgtcat attgttgttc atagccaata tcaacatctt
     8941 cctttggttc aactgtaaaa gcaggactaa atccttgctt gagttggaat tcgccaccat
     9001 atcttacaaa aaacacgagc actttattat tttcaggcaa ctcaagttca tttttaaacc
     9061 aagttactgc tgcatcagta agttctattt gcataacaat gacctcctat atttattaca
     9121 gaatgggagt gggccaactt gtattatttg tggaatttct tatcgaaatt ctctgtgttg
     9181 gagcccaccc caacctgctc attattgtaa gctgaatttc cataagcttc tatgttggga
     9241 cccccgacca gaattgaaaa aacctagtta taagcgcatt ttgttcggtt aactacagct
     9301 aatataacat tgtggagcct atgacattga tttatgtctc ggtctcaaat gttcttgtaa
     9361 aaaaactaag tatagttgaa ttttaagtta aaaaaattgt ttttgcaaaa ataaaataat
     9421 ccatcaactg caaaaaaata tgcaatgatg gactaatgtc aaaaaattgt tacatccatt
     9481 ttattttagg ttcttcgcct ctaaaaatcc ttgcgatatt agagcgatgt ctaattatca
     9541 atatgattga aactaagaaa ctaacgacta ataaaatata gtcttgaatg ataagcgagc
     9601 caatcacaca gcaaattgct gcaacgatac ttgctaaaga aacatattta aaaatcttca
     9661 atacaataaa gaagataatt gcaagtatta gtaaaagtat cggattgact cccaagacga
     9721 cacctgcact agttgcaaca gctttgccac cttggaattt taaataaaca ggataaacgt
     9781 gtccaagtat agcgaataag ccaacaatta aaccatttgt aaaaaaagta ctaatagggc
     9841 catctgcgtg aacttgtaac cataaaggga agaaaacagt tatgaaccct ttgaaaatat
     9901 ctagaaatgt taccaagaat cctgcaggac gacctaatac tctaaagcta ttagtagcgc
     9961 cagtattacc actaccaaat tgtctaatat cttttttgaa aaataatttt ccaattacga
    10021 atccacttgg gaaagcgccg ataagataac ttagtagtaa catgacgatt atcatcataa
    10081 atattacaca tcc
//
LOCUS       DAKTLH010000091        10085 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.091, whole genome shotgun sequence.
ACCESSION   DAKTLH010000091 DAKTLH010000000
VERSION     DAKTLH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10085
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.091"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(228..707)
                     /gene="rlmH"
                     /locus_tag="O0394_001861"
     CDS             complement(228..707)
                     /gene="rlmH"
                     /locus_tag="O0394_001861"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCY0499934.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(1075..3393)
                     /gene="adsA"
                     /locus_tag="O0394_001862"
     CDS             complement(1075..3393)
                     /gene="adsA"
                     /locus_tag="O0394_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HCY0499935.1"
                     /translation="MKALLLKTSVWLVLLFSVMGLWQVSNAAEQHTPMKAHAVTTIDK
                     ATTDKQQVPPTKEAAHHSGKEAATNVSASAQGTADDTNSKVTSNAPSNKPSTVVSTKV
                     NETRDVDTQQASTQKPTHTATFKLSNAKTASLSPRMFAANAPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDINKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSKSGKDTQPIGDDKVMDPA
                     KKPAPGKVVLLLAHRGTVSSGTEGSGRTIEGATVSSKSGKQLARMSVPKGSAHEKQLP
                     KTGTNQSSSPEAMFVLLAGIGLIATVRRRKAS"
     gene            complement(3621..4421)
                     /locus_tag="O0394_001863"
     CDS             complement(3621..4421)
                     /locus_tag="O0394_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0499936.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAAHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(4811..5599)
                     /locus_tag="O0394_001864"
     CDS             complement(4811..5599)
                     /locus_tag="O0394_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCY0499937.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPTSILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGAEYQLSEISSDSVKYEQTYDDFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(5600..6934)
                     /locus_tag="O0394_001865"
     CDS             complement(5600..6934)
                     /locus_tag="O0394_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499938.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLNSDFILFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(6927..8753)
                     /gene="walK"
                     /locus_tag="O0394_001866"
     CDS             complement(6927..8753)
                     /gene="walK"
                     /locus_tag="O0394_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCY0499939.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(8766..9467)
                     /locus_tag="O0394_001867"
     CDS             complement(8766..9467)
                     /locus_tag="O0394_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0499940.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
BASE COUNT         2839 a         1999 c         1545 g         3702 t
ORIGIN      
        1 gaaagaattt tctcaaagct agaactttgc ttcactataa gtattcagta taaagaatat
       61 ttcgctatta tttacttgaa atgaaagact gcggaggcta actatgtcaa aaatcatgaa
      121 cctcattact tatgataagc ttcttaaaaa cataacagca attcacataa acctcatatg
      181 ttctgataca ttcaaaatcc ctttatgaag cggctgaaaa aaccgcatca tttatgatat
      241 gcttctcctc gcataatctt aaatgctctg tacacttgtt caattaacac aacccgcatc
      301 atttgatgtg ggaatgtcat tttgctgaat gatagtgcgt agttactgcg ttgtaagacg
      361 tccttgtgca ggccgtttga tccgccaatg acgaaaacaa agtcgctttg cccttgggtc
      421 atgcgttggt tcaattcttg ggccaatcct tcggaagata gcatctttcc ttgtatttct
      481 aatgtaatga ctgtggattg tggtttgatt ttggctagta ttcgttggcc ttctttttct
      541 tttacttgct caatttcttt gtcactcata ttttctggtg ctttttcgtc tggaacttct
      601 atgatgtcta tcttggtgta tgggcctaaa cgtttttcat attctgctat ggcttgcttc
      661 caatatttct cttttagttt ccctacagct aaaatggtga ttttcatgtc gtttggtcct
      721 ccaaattgtt atcaactttc cagttatcca caagttatta acttgttcac actgttccct
      781 cttattatac caatattttt tgcagttttt gatattttcc tgacatttat gcccgatttt
      841 tagtgtataa cctgtgtatt tgtggataac tctgtgggca ttgtgggtat ttctctttaa
      901 attgtacaca attctggata actatcccca tttctgtgga taaacacctt gatgtcttat
      961 tacttatcct cgtttttata tcccatattt atcaacggtt atcgcttttt ttcataaaaa
     1021 aacactaccg tacctcttat ttaagaaata cagtagtatt attttcaata tattttagct
     1081 agcttttcta cgtcgtacag tcgcgattaa acctatacct gctaataata caaacatcgc
     1141 ttctgggctt gaactttgat tagttccagt ttttggtaac tgtttctcat gcgcgctacc
     1201 tttaggcact gacattctag ccaattgttt cccactcttg cttgatacag tagctccttc
     1261 tattgtgcga ccagaacctt ctgtaccgct actaacagtt cctctatgcg ctagcaacaa
     1321 tacaacttta cctggagctg gttttttcgc tggatccatc actttgtcgt caccaattgg
     1381 ttgtgtatct ttaccagact tactaccttt gctaccttgt tgtcctttag ctggttgttc
     1441 acttactgct ggtttaccta ataacatacg ttgtggttct gtcgtatcat acttagctaa
     1501 gttagctgtt tttaaataac ttgctagtac ttgatctaat gaaatacctt cttctctagg
     1561 accaccgaac atactatatc cgtcgccacc tgatgctgtg aagtcattca tcgttacgtg
     1621 atatacacgt tttaaatcaa tattttcaaa cttacctgtc tctttattta aaatttgaat
     1681 agcattaatt cgtttgccag acggtttatt tatatcatag taaacacgga ttgaatcaga
     1741 gatatgtagt aaaccgccat tcgctgttaa cactgtctta ccgtcctttt gtgttgttgg
     1801 tgcgcctaaa ctatgttcga aagccgtcca gacgtctgaa ccttttacat caatttgcgc
     1861 aatcgtattt ccaaatggta atactgagat taaatcatag cgtgtcacct taccttttgc
     1921 gatagaggca cgaatacctc caccatttgt cacggcaaag tcagtctttt tagagaaatt
     1981 cttaacgcca tacgcttcca tagcatctgc aatcgcgttt cctaaatttg tttcacgcgt
     2041 tctaacgtca tctctttctc ctttgaaatc aatggtattg tttggaataa ttacctctgc
     2101 agtttgtgct ctaaatgttt gatcagcttg attaatttgt tcagctaatg ctttgttcgg
     2161 tgttacattt tcaacgtctt taacattaat caatgacggt ttaatattcg atacctctcc
     2221 attgcgataa ttaaatgtaa tcttaccgat attcgcaagt gctgtacctg tttgtgccaa
     2281 tgcatcattg ttataaattt gaccattttg aagtactgta tgtgaatgac catcaataac
     2341 tgtaatacgt ttcttcaatt gtggattttg acttaattgt ttcactaagt aatcaccacg
     2401 ccatgtttct tgtgttgaag gatcaattcc taaatgtgat ataacaacaa atgtatctac
     2461 gtctttataa atacgcatca tttccgctgt cacactttgt aatggatctc taaattcaac
     2521 gcctttaatg ccttcaggtc ttgttttcgt ctttgtttct ggtgtcgtta caccaataat
     2581 tccataacga ataccatttt ttgttacaat cgttgaaggc ttaaacgcgc gttttccatc
     2641 tttataaacg ttagtactta gcatcgggaa gtctaacata ccctctaact ttttcaactg
     2701 atcgtatcca aagtcaaatt catggttacc gactgccata gcatcataac ctactgcatt
     2761 cattgcttta gccatttctt cacctttaga ctggtttgaa agtggtaaac cttggaaggc
     2821 gtctcctgcg tctaacatta aatcaggctt ttcttgttct tttactgttt ttaatttagc
     2881 cataccgatg acacgccctt tttcttcggc tagtcggcca tggatatcat ttgtatgtaa
     2941 tattttatgt gttgttgttt gtggtgcatt agcagcaaac attcgtggtg aaagtgatgc
     3001 tgttttagca tttgataatt tgaacgttgc tgtgtgagtt ggtttttgtg ttgaggcttg
     3061 ttgtgtatct acgtcgcgtg tttcgtttac ttttgttgaa actactgtag atggtttgtt
     3121 agatggtgcg ttggatgtta ctttgctgtt tgtatcatca gctgttccct gcgctgatgc
     3181 tgatacgttg gttgccgctt ctttgccaga atgatgagcc gcttcctttg ttggcggtac
     3241 ttgttgctta tctgttgttg ctttgtctat cgttgttact gcatgtgctt tcattggtgt
     3301 atgctgctca gccgcgttcg agacttgcca taatcccatt acactaaaaa gcaaaacgag
     3361 ccatacactt gttttaagta ataaagcttt catttcgttc cctccattaa ctgtcataca
     3421 ccaatatatt atcataatcc ttatattaaa atgattgatt ttatgtaaat tatttataaa
     3481 ttacatttaa tatcgaatta ttttacacat ttcaatcaag ccgattcaac tcaacaacag
     3541 ccatttccca ccaacaacat aaaaaagcac ccgataaagt cgtctcacag caatgcggaa
     3601 ctttatcgga tgactctcat ttatattgta tatattggcg ttggaatagc tttatccgta
     3661 tcacatagca atacttcttt ttccgtatca atatcgtgtt cgttcaatac ttggccaaca
     3721 ctcatacgcg ccaaatcttt catgttattg tcttgtgata aatgcgatag gtaaatacgt
     3781 ttcgtgttac ctgtaatcac gtctgtcatt gcatgagccg catcctcatt agatacatga
     3841 cccatatcgc ctaaaatacg ttgtttcgtc ttccatggat aacgacacat tctcaacata
     3901 tcgacgtcat gattactctc aaaaataaac gcatcgctgc cacgtatcat acctttcata
     3961 cgatcagaca cgtaacccgt atccgttaaa atcgtaaact tcttatagtt attatggaaa
     4021 atataaaatt gcggatctat tgcatcatgt gacacgttaa acgattcaac atcgaaacct
     4081 gcaatagatt ttgtttcata aggattaaaa atgaatttct gatccatagg gatgcgacta
     4141 tctttctttt caattgcctg ccaagtcttt tcattcgcat aaattggcaa ttgatattta
     4201 cgcgccaaaa cacctaatcc tttaatatga tcaatatgtt catgggttac taaaatacca
     4261 tttaaatctt gaatattacg gtcaatttga ctaaacaatt cttccatttt ctttccagtc
     4321 aaaccaacat caactaatag actacctttt tcattttcta caaaagtggc gttacctgta
     4381 ctaccacttg ctaatacact catgcgtatc aagcggctca tcctttctct ttaaacagtc
     4441 aatctctcta tatgactgca atatacatca gtgcaaatat acgattttcc ctacagaaac
     4501 tatcagtata ttagctgttt tgtatacctt tcctattatg taactttagt tcatcgaccg
     4561 gtttcttata tttaaatttt gataatcacg tgcaatgaat ttcttaatta ttataacaat
     4621 gattagacaa atactaaagt ttttaaaata acagttacac taattttaca atgcggttct
     4681 acttaaaata tgaataaatg cgcacgttgg agacgcaaaa acatcagcca taataaaccg
     4741 taccagcaaa aaagctagca ccgcttctca catatgatga gcttgcaatg ctagcttatt
     4801 acgattcata ttaatgatta ataattttag ggttattatt tgtcgcttcg acatagtaag
     4861 tattcgtttt atccttaccg tcatgcttca ctttaatttc ccagtttggt tgtaacaatt
     4921 gaacattcgt ttcattcacg actgagtagt aaccgagtct agcattaatg acttcatcac
     4981 cttttttcaa gtaacgatta taatataatg cctcgattgc ttttctcaca ccaatcactt
     5041 gatgcttctt atctgcacca tctgtgggct taatgtcatc catcattgat tgtttataac
     5101 tagtcgcttt gttatcttct atattaaagt tcaacatcgc tttactgtta tttaaaatcg
     5161 gaaaatcatc atacgtttgt tcatatttta cagaatctga actaatctcg cttaattgat
     5221 attcagcacc tttaaatacg cgcttgttaa cataatcttt caaatctttt aagttattgt
     5281 cactgacctt taccgattga ctaatgttcg cattcaataa ttttccacca tctgatgtgg
     5341 tcaaatccga atcgccttta gctttagaac taaagtcttt tgatcgccct gtaatttgct
     5401 ctaattttat acctttaact gatttattca atatactagt cggtacttta atttcttctt
     5461 gctgaaaatt aacttcattg ttactctcga cttcattaat gtgtgagcga ttgactttat
     5521 taacataaat cgatactaac acgatgttga caagaataaa cacgaaaatg aaaagtgtct
     5581 ttgtcagttt ccagttcatt tattcaagcc tcccatcgtt ataaacatac cattcgccat
     5641 catattctac ataccaacgc ggtacgagtt cactgttaat ctgcacttca atgtgattat
     5701 gatctgaatt atcctgcatt tcgtaaccga tagcgatgtt tgttactttt tcaaaattaa
     5761 tatcactatt gttcgctaag cttgaacgta cagactctaa tttcggcaac gatttattat
     5821 cctcactatt taaaacaacg tcggtgcgta ataacgaacg acgatagtca aagacgcctt
     5881 tttcacccca agtgacttga atttgattag aaccttcttt attaaacgtt ggataaccat
     5941 taaggaaacg ttgatatgtt aactcgcctg actgattatt cgtactaaac aatctaaagt
     6001 cttcgtttaa gaaaccacca tgaccattaa taaaatcaaa ggttcctgga atcgtttctt
     6061 ccattttgct ggaactcgct tcatcttcgg acaggttttt ataatgatat ttttcatttt
     6121 tatcgttata gtttgcgaca cctgtattat tgttgtaggt tgtaacacca ctctttgaac
     6181 tacgaacgat ggttgaatcg tcaaatagta tagcattcat tttctcaaca ctaatcgtgt
     6241 taaataccat gcgatatgtt tttaactttt caggtttact tggtgcaaaa acatgcgtcg
     6301 tacgatcaat tgtatctttg tttgtgatga tatctgtgta tggttgcata tcttttttca
     6361 ctgctgctaa tgcatctaaa aaatgatcat ttttcgttgt agttgttaat tttacgtaat
     6421 cgtggcgatc tttgcttata gcataaagca cgatattatc atcagcatca tgatctatga
     6481 ccaaacgatt gaaattgaaa tgatttggta ctttcgcatt catgttcagt acttgaccaa
     6541 gatatgttga taacggtaaa tcatacgtaa aatcgaataa tataaaatca ctgttcaaat
     6601 caggaatcat caagttatga tcacgacgaa catgttccac ggacttcact tctttatttt
     6661 tcaaaggttt cgtcagttta ttcacattag ataccgtcgc aatcgttcct tctggatgat
     6721 catttttcga atgaataatc tgaaatggcg taatagttgt atccattttg gctgtcatag
     6781 gtgtcgttaa aggtttcgtt tcactcttct tactatctgt attgtcgaca tttgcaatat
     6841 caggagaaaa gttccatacc atatatgtca atacgacact catcaagacg agtagtgcta
     6901 aaatgacaga tttaatatgc tccttattat tcatcccaat caccgtcttc aatgacttca
     6961 catggaagtg tgataaagat agatgtacct tgaccttcta cactgtttgc ccaaatacga
     7021 ccattgtgcg cttccacaat ctctttcgaa atggctagtc ctaatccagt accacccatt
     7081 ttacgcgtac gtgccttatc tacacgatag aatcggtcga atatcttatc gactttattg
     7141 ataggaatac caatgccatt atctttaata cgaatcgtca ttcgattata aagtggattt
     7201 tgtttcacgt ggaactcgac acgtttatcg cctctagaat atttcatcgc atttgtaatg
     7261 acattatcaa atacttgcgt cattttatca ggatcaaatt ctgtgaaaat cgtctttttc
     7321 ggaatatctc gaataaatgt tgtatctttc gcagacattt catgtcgatt aataatttta
     7381 ttaatgaaca tgttaaagtc gataatttct ttgttgattt gatcagactc attatccatt
     7441 ttagataact gtagcaagtc attgaccagt cgaatcattc gttctgtttc ttcacgggta
     7501 acagataaaa attgtggcgc aagttcctca tctttccatg caccttcttc aagtgcttca
     7561 atgtaactat tcatagaagt taaaggtgta cgtaactcat gtgatacatt ggcaacaaat
     7621 tcacgacgct cacgttcaac ttgttgttgt tcagttacgt catgtaacac agcgatataa
     7681 ccagttacaa atcctgtttc ctgcacaatc gtactaaagt taacacgtgc gattagacct
     7741 tcttcttcat ttaaatctaa taagaaacta tcattattct cttgaatttc ttccagttta
     7801 aattcatctt caagacttaa tacacttaac atgtaatatc cgatgatgtc ttctttcgcc
     7861 ataccaagca tcttgagtgc catatcattg acgatacgaa tacgtccacg gcggtctgtt
     7921 gcaataatac catcactcat atgggtgata actgagtcca gtctacgttt ctcactttca
     7981 gtattagcct gcgcttcttg tacacgttta gacaagttat taaatgctaa agctaattcg
     8041 ccaatttcat cattaccata aatcttcaca cgttgcgtat agttacctct ggacatttcg
     8101 accgtctggt tacgcatatc ggtgattggt ttggtaatcg ttcgcgctat aaagaatcct
     8161 aggatgactg tgattaataa tgaaatagct gtaccaacaa tgaatatttg atttatatta
     8221 tttaattggt tataaacgtc attaattttt gattcgatat aaatattacc aattaccttt
     8281 ttatcgactt taactgggat attatatacc cagacacggt ccttaccacc gccataatct
     8341 tttaaaatta aatgatcgtt tgattgtcct agtgatagtg ctttttggac agaactatca
     8401 ttcgcttttt gattgattag actacggtta gactgcttcg tcgtcgcaat aataatttgg
     8461 tctttatcta taaaacgaat ttctccaatt tcttgacggt tggcatactc acttaataaa
     8521 ttttgaatat ctttttgtgc atttacggag cccttttcgt catatacttt ttcaatacta
     8581 atttctaatt gtttcgcgta ctgcgtaata ttcttcttaa aattatcaag cagctctttt
     8641 tcaaggttat ttgtaaaata taacccgata atttgcatac caatgataat cagtaataca
     8701 taaacaatta caagtttagt atgaagggat tgtagttgtt ttagccactt cattcgtttc
     8761 gacctctact catgttgttg gaggaaatat ccaacgcctc tacgcgtcac aatatattca
     8821 ggatgtgacg gatcatcttc aatcttttca cgtaaacgac gaatcgttac atcgaccgta
     8881 cgtacatcgc caaagtaatc atagccccat actgtttgta ataaatgttc acgtgtcatt
     8941 acttgtccca tatgttttga taaataatgg aacaattcaa attcacgatg tgttaattca
     9001 atatcttcgc cacgtttttt aatagaatat gcgtctggat aaatcacaat atctttaatt
     9061 gtgatttcat tcgttacatt tccagtgtct tgtgctggtt gtgagtaatg acgacgtaag
     9121 ttcgctttca cacgtgcgat taattcacgc gtactaaacg gtttcgttac atagtcatct
     9181 gcacctagtt ctaaaccaag cactttatca atttctgaat ctttagcagt aagcattatt
     9241 attggcattt cgtatttttt gcgcacttca cgacatactt ccataccatc acgaccaggt
     9301 aacatgatat ctagtaatac gatgtctggt tcttcttcat aaattaagtc gactgcatca
     9361 ttaccatcgt atgcacagta cacatcgtat ccttcttttt ttaagttaaa ttctaaaata
     9421 tcagcaatcg gtttttcatc atcaactaca acaacttttc tagccatttg cataaacctc
     9481 ttttcttaaa tcattaatat tattttatca ttttttgttc tttatcgttt atgtttgtgt
     9541 aaaaaatcac aggatgtcgc ttactatttt tgttattgtg cgcattttcc gtcttataac
     9601 gtatagtaac ctattatata atttaccatt tataagctat taattctatt ttccaatata
     9661 aacttatata ttactatttt ttattagttt ctcacggcgg attgtgacaa tgatatttaa
     9721 cacaatgatg aggtctctat tccaataata aaattaggat aatggcaact tctagttgca
     9781 cgtcattgct cagaaaataa atatcattat tcgttattcg ttactttaac aattgatgtt
     9841 atggaggact ggtggcatat cacaacgaag aaatatgttg acagtagcat atgtttgtac
     9901 gtctaataaa gattttattg tctacgttta tctttcatat ccaaccacta agttacttga
     9961 ggttcataca ttttaaagaa aatggacgaa cagataaatc aatatgatta tcattcgtcc
    10021 atatatcttt tattaatata atattaaacg caaaaaaatg cctatcctct tggataggca
    10081 tttgt
//
LOCUS       DAKTLH010000092        10018 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.092, whole genome shotgun sequence.
ACCESSION   DAKTLH010000092 DAKTLH010000000
VERSION     DAKTLH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10018
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.092"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            90..1550
                     /gene="ebpS"
                     /locus_tag="O0394_001868"
     CDS             90..1550
                     /gene="ebpS"
                     /locus_tag="O0394_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HCY0499941.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHDTIKNAENNTEHSTVSDKSEA
                     EQSQQPKPYFTTGANQSETSKNEHDNDSVKQDQDEPKEHHNGKKAAAIGAGTAGVAGA
                     AGAMAASKAKKHSNDAQNKSNSGKANNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            1675..1791
                     /locus_tag="O0394_001869"
     CDS             1675..1791
                     /locus_tag="O0394_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499942.1"
                     /translation="MLFNKVFSRILAYQFNFLNSHQKDKTPQSTNLTRKNKK"
     gene            1952..2938
                     /locus_tag="O0394_001870"
     CDS             1952..2938
                     /locus_tag="O0394_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HCY0499943.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES"
     gene            complement(3053..4021)
                     /locus_tag="O0394_001871"
     CDS             complement(3053..4021)
                     /locus_tag="O0394_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCY0499944.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            4098..4757
                     /locus_tag="O0394_001872"
     CDS             4098..4757
                     /locus_tag="O0394_001872"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCY0499945.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQI
                     K"
     gene            4788..4904
                     /locus_tag="O0394_001873"
     CDS             4788..4904
                     /locus_tag="O0394_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499946.1"
                     /translation="MQRLTHIQNECADNDLLKLKGDFYSMMNESCLFIFGKS"
     gene            5021..5212
                     /locus_tag="O0394_001874"
     CDS             5021..5212
                     /locus_tag="O0394_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499947.1"
                     /translation="MSVTNLNIAKEADLVTKNVLCNNSRKMHYIRSSYYSNVKVAYLR
                     GFLFYKSIKFYYNISCREQ"
     gene            5469..6644
                     /gene="rpsA"
                     /locus_tag="O0394_001875"
     CDS             5469..6644
                     /gene="rpsA"
                     /locus_tag="O0394_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCY0499948.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL"
     gene            6866..8176
                     /gene="der"
                     /locus_tag="O0394_001876"
     CDS             6866..8176
                     /gene="der"
                     /locus_tag="O0394_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCY0499949.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEAYVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTGAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            8193..9191
                     /locus_tag="O0394_001877"
     CDS             8193..9191
                     /locus_tag="O0394_001877"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCY0499950.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            9362..9634
                     /locus_tag="O0394_001878"
     CDS             9362..9634
                     /locus_tag="O0394_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCY0499951.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
BASE COUNT         3737 a         1437 c         1864 g         2980 t
ORIGIN      
        1 gaaataattt tgaaaaatat acataaacat atgtcatgtg ggtatatttt atgtaaaatc
       61 attgtaatag aatagaaagg aagatggcta tgtctaataa ttttaaagat gactttgaaa
      121 aaaatcgtca atcgatagac acaaattcac atcaagacca tacggaagat gttgaaaaag
      181 accaatcaga attagaacat caggatacaa tagagaatac ggagcaacag tttccgccaa
      241 gaaatgccca aagaagaaaa agacgccgtg atttagcaac gaatcataat aaacaagttc
      301 acaatgaatc acaaacatct gaagacaatg ttcaaaatga ggctggcaca atagatgatc
      361 gtcaagtcga atcatcacac agtactgaaa gtcaagaacc tagccatcaa gacagtacac
      421 ctcaacatga agaggaatat tataataaga atgcttttgc aatggataaa tcacatccag
      481 aaccaatcga agacaatgat aaacacgata ctattaaaaa tgcagaaaat aacactgagc
      541 attcaacagt ttctgataag agtgaagctg aacaatctca gcaacctaaa ccatatttta
      601 caacaggtgc taaccaatca gaaacatcaa aaaatgaaca tgataatgat tctgtaaaac
      661 aagatcaaga tgaacctaaa gaacatcata atggtaaaaa agcagcagct attggtgctg
      721 gaacagcagg tgttgcaggt gcagctggtg caatggctgc ttctaaagct aagaaacatt
      781 caaatgacgc tcaaaacaaa agtaattctg gcaaggcgaa taactcgact gaggataaag
      841 cgtctcaaga taagtctaaa gatcatcata atggcaaaaa aggtgcagcg atcggtgctg
      901 gaacagcagg tttggctgga ggcgcagcaa gtaaaagtgc ttctgccgct tcaaaaccac
      961 atgcctctaa taatgcaagc caaaaccatg atgaacatga caatcatgac agagataaag
     1021 aacgtaaaaa aggtggcatg gccaaagtat tgttaccatt aattgcagct gtactaatta
     1081 tcggtgcatt agcgatattt ggaggcatgg cattaaacaa tcataataat ggtacaaaag
     1141 aaaataaaat cgcgaataca aataaaaata atgctgatga aagtaaagac aaagacacat
     1201 ctaaagacgc ttctaaagat aaatcaaaat ctacagacag tgataaatca aaagaggatc
     1261 aagacaaagc gactaaagat gaatctgata atgatcaaaa caacgctaat caagcgaaca
     1321 atcaagcaca aaataatcaa aatcaacaac aagctaatca aaatcaacaa cagcaacaac
     1381 aacgtcaagg tggtggccaa agacatacag tgaatggtca agaaaactta taccgtatcg
     1441 caattcaata ctacggttca ggttcaccgg aaaatgttga aaaaattaga cgtgccaatg
     1501 gtttaagtgg taacaatatt agaaacggtc aacaaatcgt tattccataa tataactata
     1561 taaattgtaa ctgaactgct atatacaaac gtgcatatta taagtatctc taaatatttt
     1621 ggagaactta aattgcgctt ataagtatgt agcggttttt tcatttttca aagtttgtta
     1681 tttaacaagg tcttttctcg aatattggca tatcaattta actttttaaa tagtcatcaa
     1741 aaagataaaa caccacaatc aacaaattta acgaggaaga ataaaaaata aatcaacata
     1801 ttaaattgta gtgttattca actccgtagc taacaattct ctatgcacat taaacaaatt
     1861 gtcaaaaata tatcataaat cttcaagcac agacttagcg catcaatcac tgaactgtta
     1921 taatagtttg ggattaaagg aggccgaaac aatgcaaaaa gttgaaagta tcataattgg
     1981 tggagggcca tgcggattaa gtgcggctat tgaacaaaaa agaaaaggta ttgatacctt
     2041 aattattgaa aagggtaatg tcgttgaatc aatctacaat tatcctactc accaaacatt
     2101 tttctcatca agtgataaat taagtattgg ggacgtaccg tttatcgttg aagaaagtaa
     2161 accaagacgt aatcaagcgc tagtttatta ccgagaagtt gtaaaacatc atcaattaaa
     2221 agtaaatgca tttgaagaag tattaactgt taaaaaaatg aataataaat ttactattac
     2281 tacgacgaaa gatgtttatg aatgtcgatt tttaacaatc gcgacaggct attatggtca
     2341 gcataataca ttagaagttg aaggtgcgga tttacctaaa gtgttccatt attttaaaga
     2401 ggcacatccg tattttgatc aagatgttgt aattatcggt ggtaagaatt cggctatcga
     2461 tgctgctttg gagttggaaa aagctggtgc taacgtgacg gttctatatc gtggtggaga
     2521 ttattcgcct tcaattaaac cgtggatact tccaaatttc acagcattag taaatcatga
     2581 aaaaattgac atggaattta atgctaatgt tacccaaata actgaagata ctgtgactta
     2641 tgaagtaaat ggtgaaagta aaacgataca caatgattat gtatttgcga tgattggtta
     2701 tcatcccgat tatgaatttt taaaatctgt aggcattcaa attaatacaa atgaatttgg
     2761 aacagcgcct atgtataata aagaaacata cgaaacaaat atcgaaaatt gctatattgc
     2821 aggtgtaatt gctgcaggga acgatgcgaa taccattttt attgaaaatg gtaaattcca
     2881 cgggggcatt attgctcaaa gcatgctagc taagaaacaa acgcccttag aatcataaaa
     2941 ataaaggtct atgtactaaa taacttagtt ttacaacgac tgacattcat gatatgtcaa
     3001 atgaggttga tgactaatga ttgtacatag acctttttat gttacgtatt cattataatt
     3061 caaaatatga tttgatttca gctttatcta aattgttgct taacgcgact aataatttta
     3121 atcttgcttt tggaccattc aagccgttag aaaaaataaa accttgttgt gcgagttggt
     3181 aaccaccacc atcgtatgcg taagttggac tcacaatacc attaaaggaa cgtgaaacta
     3241 gcacaatagg tatatttaaa gatactaatt gttgaatgcc ttctaatgcg cttggaggta
     3301 tgttgccttg tcctaacgct tcaataacca taccatcgat accttctcga ctataaaaac
     3361 taaaaatgtc acctggcata cccatatatg cttttactaa tggtacattt agtttgtcat
     3421 tgacattttc caatgcttgt tggcgatatg gcatatgatg gaattgcaca cgatcctttg
     3481 tcaatacacc tagcggacca tgatttggac tttgaaatgt gtttgtatta gacgtatgtg
     3541 ttttggtaac attacgcgcc gtatgaattt catcattaaa tacaaccatc acgcctttat
     3601 gacgggcctt ttcatcagag gcaacgcgaa tagcggaaat ataattatat aatccgtcag
     3661 aaccaatttc attagacgag cgcattgcgc cagtaataac aacaggttgc tcgataccta
     3721 atattaaatc aagtaaaaag gcagtttctt ctaacgtatc ggtaccatgc gtgataacga
     3781 aaccatcata atatttattt gttactgctt ctaaaataat atcctttaat tgtttaacat
     3841 gttggattgt catatgaggt gatggtacat taaaaggatt taattcatct atttgtgcat
     3901 attgatttat gacatcttga tgcattgaaa tagggttgat atcatttgtt actactttat
     3961 tagattggtc ttgtgacata ctaatggtgc caccagtatg aataacaagt agatgtttca
     4021 tatatttcct cctatattta atttacctaa ttatgataaa atattattca taaaacgaca
     4081 aggaagggaa atgacgcatg aaagccatta atattgcatt agatggtcca gctgctgccg
     4141 gaaaaagtac aattgcgaaa cgtgtagcca gcgaactatc aatgatttat gtcgatacag
     4201 gagcaatgta tcgtgcatta acatacaaat atttaaaatt aaacaaaact gaggactttg
     4261 caaaactagt tgaccaaaca acattagatt taacttataa agcagataaa ggtcaatgtg
     4321 tcattttaga taacgaagat gtaacagact ttttaagaaa taatgatgtg acgcaacatg
     4381 tttcatacgt tgcatctaaa gagccagtac gttcattcgc cgttaaaaaa caaaaagagt
     4441 tagctgcaga aaaaggtatc gtaatggatg gtcgcgatat cggaactgta gtgctaccag
     4501 atgcagattt aaaagtatat atgattgcat cagttgaaga gcgagcagaa agaagatata
     4561 aagataatca attaagaggt atcgaatcaa attttgaaga tttaaaacgt gatattgaag
     4621 ctcgtgatca atatgacatg aaccgtgaaa tatcaccatt aagaaaagca gatgatgcag
     4681 tgacattaga tacgacaggc aagtcgattg aagaagttac tgacgaaatt ttagcgatgg
     4741 tgagtcaaat taaataatct aataagtcat aactatctat ttgtaatgtg caacgcttaa
     4801 cgcatataca aaatgaatgt gctgataatg atttactcaa attaaaaggt gatttttatt
     4861 caatgatgaa tgaaagttgc ctttttattt ttggtaaaag ttaatgcgtc agtgaattgt
     4921 gtaagttttt caaaaagtaa aaagaaataa taaaggtgaa ttattagaat tccaaaaata
     4981 attcattaca ttcataaagc attttacaaa tggtaagaaa atgagtgtta caaatctaaa
     5041 tattgcaaaa gaagctgatt tagtcacaaa aaatgtccta tgtaataatt cgagaaagat
     5101 gcactatata cggtcttctt actattcaaa tgtaaaagtt gcttatttgc gtggcttttt
     5161 gttttataaa agtataaaat tttactataa tatatcttgt agagaacaat gaaatgacag
     5221 acttataaaa caaaatagta agctatgcta atttttatac aaaaaatatg tataaacata
     5281 gcaaactgta ttactttgat acaaaaatgg ttgtaataaa tatttatcga tatgacgact
     5341 tgaatatgat aaagtgacat atttatgtat atgactattt cgcaaaatgt aatcgaggta
     5401 gaatttcttg acaattctgt cagtttataa gatgttataa atatgtagtg tataaggagg
     5461 caaacaagat gactgaagaa ttcaatgaat caatgattaa cgatattaaa gaaggtgaca
     5521 aagtcactgg cgaggtacaa caagttgaag acaagcaagt tgttgttcat atcaacggtg
     5581 gtaaatttaa tgggattatt cctattagtc aattatctac gcatcatatt gatagcccaa
     5641 gtgaagttgt aaaagagggc gacgaagttg aagcatatgt cactaaagtt gagtttgatg
     5701 aagaaaatga aactggagct tacatcttat ctagaagaca acttgaaact gagaagtctt
     5761 atagttattt acaagaaaaa ttagataata atgaaatcat cgaagcgaaa gtaacagaag
     5821 tagttaaagg tggtttggtt gttgatgtag gacaaagagg ttttgttccg gcttcactaa
     5881 tttcaacaga cttcattgag gatttctctg tgtttgatgg acaaacaatt cgtattaaag
     5941 ttgaagaatt ggatcctgaa aataatagag tcattttaag ccgtaaagca gttgaacaag
     6001 aagaaaacga tgctaaaaaa gatcaattat tacaatcttt aaatgaaggc gatgttattg
     6061 atggtaaagt agcgcgttta actcaatttg gtgcatttat agacattggc ggtgttgatg
     6121 gtttagtgca tgtatctgaa ctttctcacg aacatgttca aacaccagaa gaagtagttt
     6181 caattggtca agatgttaaa gttaaaatta aatctattga tagagataca gaacgtattt
     6241 cattatcaat caaagatacg ttaccaacac ctttcgaaaa tattaaaggt caattccacg
     6301 aaaatgatgt cattgaaggt gtcgtagtaa gattggcaaa ctttggtgca tttgttgaaa
     6361 ttgcaccagg tgtacaagga cttgtacata tttctgaaat tgcacacaaa cacattggta
     6421 cgccaggtga agtgttagaa cctggtcaac aagtaaatgt taaaatatta ggtattgatg
     6481 aagagaatga aagagtatca ctatctatta aagcaacatt accaaacgaa gatgttgttg
     6541 aaagtgatcc ttctacgact aaggcgtact tagaaaacga agaagaagat aatccaacaa
     6601 ttggcgatat gattggtgat aaacttaaaa atcttaaact ataatttaat atttaatagt
     6661 caactccaca tgtttatgat tgcatgtgga gtatttttat gtaacaaaat atactcggaa
     6721 tgataacgtg gacaaattta actaagtgtt taaaaagata aagttttaag tgctgatttt
     6781 tatcattaca gtaataaact cattttgaat acacagtctc atgtgatatt attaaaaaga
     6841 tataagaaag agaggaagtt agcttatgac taaacctata gtagctattg taggtaggcc
     6901 taatgtaggt aaatctacaa tttttaatag aatagttgga gaacgtgttt cgattgtgga
     6961 agacacgcca ggtgtaacac gagatcgtat ttattcttca ggtgaatggt taacacatga
     7021 tttcaatatt attgatacag gtggtattga aattggtgat gcaccattcc aaacacaaat
     7081 tagagcgcag gcagaaatcg ccatagatga agcgtatgtt attattttta tggttaacgt
     7141 gcgtgaagga ttgacacaaa gcgatgaaat ggtcgctcaa attttataca aatctaaaaa
     7201 accggtcgta ttagcggtta acaaagtaga taatatggaa atgcgtacag acgtgtatga
     7261 tttctattca ttaggatttg gtgaaccgta tccgatatca gggtcacatg gtttaggtct
     7321 tggtgacttg ttagatgcag ttgtttctca ttttggtgaa gaggaagaag atccttatga
     7381 tgaagataca attcgactat ccattattgg acgaccaaac gtaggtaaat caagtttagt
     7441 aaatgctatt ttaggtgaag atcgcgttat cgtttctaat gttgcaggga caacgagaga
     7501 cgctattgat acagagtata gttatgatgg acaagattat gttttaatcg atactgctgg
     7561 tatgcgtaaa aaaggaaaag tatatgaatc aactgagaaa tattcagtat taagagcttt
     7621 aaaagcgatt gaacgttcaa atgttgtttt agtggtcata gatgcagaac aaggcatcat
     7681 tgaacaagat aaacgtgttg caggatatgc acatgaacaa ggtaaagcag tcgtgattgt
     7741 cgtaaataaa tgggatactg tggaaaaaga tagtaaaacg atgaagaaat ttgaagatga
     7801 agtacgtaaa gaattccaat ttttagatta tgcacaaatt gcttttgtgt ctgctaaaga
     7861 acgcacaaga ttacgtacat tattccctta catcaatgaa gcaagtgaaa accataaaaa
     7921 acgtgttcaa agttcaactt taaatgaagt tgttactggt gcaatttcca tgaaccctac
     7981 accaacagac aaaggtagac gtttgaatgt cttttatgca acacaagttg ctatagaacc
     8041 accgacattt gttgtatttg ttaatgatgt agaattaatg catttttctt ataaacgcta
     8101 tttagagaat caaatccgtg ccgcttttgg ttttgaaggt acaccaattc atattatagc
     8161 tcgaaagaga aattaacgat tgggggataa caatgactaa aattaccgtt tttggtatgg
     8221 gaagttttgg gacagccctt gccaatgttc ttgcagaaaa tggacatgat gttttgatgt
     8281 ggggtaaaaa tcaagatgct gttgatgaat taaatacatg tcatacaaat aaaaagtatt
     8341 taaaatacgc gaaattagat gttaacatca tcgctacttc agatatgacc aaagcaattc
     8401 aatttgcaga tatttactta atggctttac ctactaaagc aatgcgagaa gttgcttctc
     8461 aaattaatga taagctgacc tctaaaaaga cttttataca tgttgctaaa ggtattgaaa
     8521 atgggacgtt taaacgtgtg tcagaaatga ttgaagattc tatttcacct gaatataatg
     8581 caggtattgg cgtgttgtca gggccaagtc atgcggaaga agttgtagtc aagcaaccaa
     8641 ctacagttgc tgcttcatca aaagataaaa gtgtaagtaa attaacgcaa gatttattta
     8701 tgaatgatta tttgcgtgtg tacacgaatg atgacttgat tggtgttgaa cttggtggtg
     8761 cattgaaaaa tatcatcgca gtagcaagtg gtatcgtagc tggaattggc tacggtgata
     8821 atgcaaaagc tgcattaatg actcgtggct tagcggaaat tagtagatta ggtgaaaagt
     8881 taggtgccga tcctatgaca tttctaggtt taggtggtat cggtgactta atcgttactt
     8941 gcacatcaac acattctcgg aatttcacat taggatataa acttggacaa ggtgaatcaa
     9001 tggatcaagc attatctgaa atgaatatgg ttgttgaagg tatttataca actaaatcag
     9061 tttatcattt agctaaagaa aaaaatgtgg atatgccaat tacaaatgca ttatatagag
     9121 tattatttga aaatatctca gtaaaagaat gcgtaaaaga tttaatggag cgcgataaaa
     9181 aatctgaata aaatgtgtat tattacacat atacggctaa taattacgta aaatgaatag
     9241 aaatggctta aattcaacgt ttttgccaca taaatgattg cgatttatgt aatgcttgtg
     9301 ttaaagtttt accataatga tataaacata tcattatcaa acctcaggag gtgaatgtct
     9361 aatgaacaaa acagatttaa tcaatgcagt tgcagagcaa gctgatttaa ctaaaaaaga
     9421 agctggttca gcagtagatg ctgtattcga atcaatccaa aactcacttg ctaaaggtga
     9481 aaaagtacaa ttaattggtt tcggtaactt tgaggtacgt gaacgtgctg cacgtaaagg
     9541 tcgtaaccct caaactggta aagaaattga tatcccagca agtaaagttc cagcattcaa
     9601 agctggtaaa gcattaaaag atgctgtaaa ataattttac ttaaaaagcc ctgaataagg
     9661 gctttttatt ttgcttttaa tacttacaac ctgtacataa attgtaatgt tcttctaagt
     9721 ttttaatctt tggaacattc attgtttaag cgttcaaaat attacatgac ataatagttt
     9781 tgataaaaat agcaactaca aaatatacat atgaatattg agattaattg ttagcgttga
     9841 atttacttaa aaggtaacca tgtctactat agtattttac gttatttaaa aagatgaata
     9901 atgtaaatga agtaaaggtt attatgagaa ttacaaaagc tacataaatt actgttagtt
     9961 taaattgaaa tttaaaaatg ataagtaatc ttaatgcttc aaaagatttt aatttctt
//
LOCUS       DAKTLH010000093         9911 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.093, whole genome shotgun sequence.
ACCESSION   DAKTLH010000093 DAKTLH010000000
VERSION     DAKTLH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9911
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.093"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..655)
                     /locus_tag="O0394_001879"
     CDS             complement(<1..655)
                     /locus_tag="O0394_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCY0499952.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPN
                     PEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDY
                     "
     gene            complement(779..913)
                     /locus_tag="O0394_001880"
     CDS             complement(779..913)
                     /locus_tag="O0394_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCY0499953.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            complement(970..1104)
                     /locus_tag="O0394_001881"
     CDS             complement(970..1104)
                     /locus_tag="O0394_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCY0499954.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            1468..1541
                     /locus_tag="O0394_001882"
     tRNA            1468..1541
                     /locus_tag="O0394_001882"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1502..1504,aa:Arg,seq:tct)
     gene            complement(2016..2204)
                     /locus_tag="O0394_001883"
     CDS             complement(2016..2204)
                     /locus_tag="O0394_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0499955.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANSIFSNEN"
     gene            2453..3400
                     /locus_tag="O0394_001884"
     CDS             2453..3400
                     /locus_tag="O0394_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCY0499956.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMIKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLKNVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     IFLLLAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPDIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKEKQDKFLRNASQ"
     gene            complement(3705..3932)
                     /locus_tag="O0394_001885"
     CDS             complement(3705..3932)
                     /locus_tag="O0394_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499957.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVAYANMVFDWNLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
     gene            complement(4240..5796)
                     /locus_tag="O0394_001886"
     CDS             complement(4240..5796)
                     /locus_tag="O0394_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCY0499958.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
     gene            complement(5968..6900)
                     /gene="arcC"
                     /locus_tag="O0394_001887"
     CDS             complement(5968..6900)
                     /gene="arcC"
                     /locus_tag="O0394_001887"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCY0499959.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            complement(6923..7924)
                     /gene="argF"
                     /locus_tag="O0394_001888"
     CDS             complement(6923..7924)
                     /gene="argF"
                     /locus_tag="O0394_001888"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCY0499960.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEIMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            8362..9087
                     /locus_tag="O0394_001889"
     CDS             8362..9087
                     /locus_tag="O0394_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HCY0499961.1"
                     /translation="MKKNIMNKLVLSTALLLLETTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHFSLSGKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQL
                     RNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGH
                     EFYKGNKPVLTLKELDFRIRQTLIKNKKLYNGEFNKGQIKITADGNNYTIDLSKKLKL
                     TDTNRYVKNPRNAEIEVILEKSN"
     gene            9182..9907
                     /locus_tag="O0394_001890"
     CDS             9182..9907
                     /locus_tag="O0394_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HCY0499962.1"
                     /translation="MNNNITKKIILSTTLLLLGTASTQFPNTPINSSSEAKAYYINQN
                     ETNVNELTKYYSQKYLTFSNSTLWQKDNGTIHATLLQFSWYSHIQVYGPESWGNINQL
                     RNKSVDIFGIKDQETIDSFALSQETFTGGVTPAATSNDKHYKLNVTYKDKAETFTGGF
                     PVYEGNKPVLTLKELDFRIRQTLIKSKKLYNNSYNKGQIKITGADNNYTIDLSKRLPS
                     TDANRYVKKPQNAKIEVILEKSN"
BASE COUNT         3354 a         1775 c         1328 g         3454 t
ORIGIN      
        1 cataatcagg tataattcct ggattatgat caaatcctct aaaattaaac cagcaagtag
       61 ctatacccgc attgattcca cctagaatgt cagatgttaa agaatctcca actataatcg
      121 agtgctgtct ttcatcctca ccaatatcat taaaaacata attaaaaaat tccggattag
      181 gtttttgata tcctgtttct tcagatataa atatcttttt aatatattta tgcaacggcg
      241 tctgatttaa ccttctcttt tgcgtttcgg ttacaccatt agtaacaata tataaatcat
      301 gtcttttcga taattcgaca attgtttcta atgtttgatc aaagtattta actttagctt
      361 ctgctaatcc atttctaaat aacacatctg cacgatgccc atcaacttcc atttgatgat
      421 gtttgaagta attcacaaat cgttctgata atacttcaga cttcgttaat ttattttgtt
      481 gaaaagcttc ccaatgttgg tgattgattt ttttaaatgt taaaaaatca tcctttgttg
      541 ctttatgatt aaaaacattc gccatatagt gaaacgccca ttcttctgca tcataaaaat
      601 caacaattgt atcatcaaag tctatcaaaa tatttttata tcccaatttc cccatctcct
      661 atattgtcta tgtatctaaa tcttaacaga ggctcaaatt tctgcaaata aaataaactg
      721 agtgcataac attaaagtat gctcacccag tttattttaa agaatattag ttattatatt
      781 agaatccaaa taatttacct agtaaaccca caccgttagc aacgatgtct acgatacttg
      841 tgcctaattt cacactatca tgttgttgtg cagcttgcac agtatttgcg attgcttctg
      901 ctagtccagt catttaaatc tctccctcac ctttgaaata atactgatta cttacataac
      961 atattgaaat tagaatccga ataatttacc taataaacct acgccatttt caacgatgct
     1021 cacaatgctt gtgcctaatt ttgcgccatc attattaatt gctgcagtta cggtatcttt
     1081 aattgcgtta aataaacctt ccattgaaaa cactccttaa aatttaaatt tgaagataac
     1141 aaaaacgtgc gtagctttta aatcaccgaa atgttattcg cttatcgttt tgttgttgtt
     1201 attttaaaat aaatttgata caattagttt gtttatccgc acaacatctt ataatgtact
     1261 taactgtatt ttaaagagaa aagaaataca gttaggcatt caaaactgta tttaacacaa
     1321 ttaagttgcc tgaattcgta tttaagtctt attgaacctt tttagataaa tagctctata
     1381 atagtgaaaa atataaacat tttttattta caaggtattg ctaatttaag ttcatttaga
     1441 tataataatt cttgtgttgt taaacgtgtc ctggtagctc agctggatag agcaatggcc
     1501 ttctaagcca tcggtcgggg gttcgaatcc ctcccaggac gtttataggt atttttatac
     1561 gcattaccaa acaaaagagt tccgtgatta cggggctctt tttgttttga atttcagtaa
     1621 tatagtatga tgcgtcacca aaacgtcccc cgcataagcc ccgaaaatac agtaattaaa
     1681 acaagcatgc ttattcgtta tagaattttt tgacacacaa ttgacacgcg tctgacactt
     1741 gtttatacat ttttaattaa gtaattttgt gctcaaattt catctatact gcacctgaac
     1801 tacaccaaca ctacaccaag atttttaaca ctcaccattt gcatgcgtag agatttttat
     1861 tattatatta ttcctataga ttttgatact attcaaaatt ttagggactt ttcaggggcc
     1921 cgaaatccta taattataat tatatacatc taaaaaaaat aaccacgtcc atcgagacgt
     1981 ggttttttaa ttatttaact cactaattat tcttcttaat tttcattgct aaatatacta
     2041 ttggcaaatt tataaagtag ttcagcgttt ttcaatgaca tattgtctaa tgatctttca
     2101 ttttttctca ttctgtgtat tgtgctttgt ggaactcctg tttgtttcga tatatgtaaa
     2161 ctgctcaaat cactgtctaa tagtttttga atttgatttc tcattttgta cacccccttg
     2221 taaatcttca atcatcattt aaattaaata attacttttc cacatattcc aatatttagg
     2281 ttgcaaagca tacctcaaat atcattaatt ttgagattta aatgtcaatt atgtgtctat
     2341 caatccaata tacatactct aataacgtaa tagtacacac tcttcttatt aattggccat
     2401 agctatcatg atataattag tgaagaaaat cacataagaa aggttgtaaa tcatgagact
     2461 tcaaaaagca cctctagtaa cgtcaggact agtcttagga ttattaggcc tgggtaatct
     2521 attaaaagac ttatctctta ctttaaacgc tgtttgcgga atctttgctt tcttgatttg
     2581 gattcacctt ttatgtacta tgatcaaata ttttaataat gtgaaagaac aattaaacag
     2641 tcctctagtt tcatcagtgt tcacaacatt tttcatgtct ggctttttag gtactactta
     2701 tttaaataca ttttttagta acataacttt tatcaatagc ttaataacgc ctatttggat
     2761 tttatgcctt gtgggaatta tgacgcatat gattattttt tcaataaaat atttaaaaga
     2821 tttttcactt aaaaatgttt atccttcgtg gactgtactt tttattggta ttgctatcgc
     2881 aggattgacg gcacccgtta gcggatattt tttcataggt caattaacag taatatatgg
     2941 ctttgtagct acttgtattg tcttacctat agttttcaag cgattaaaag catttccatt
     3001 gcagacgtca atcaaaccga acacatcgac aatttgtgca ccattttctt tagtcgctgc
     3061 agcatatgtt atagcttttc ctaaggcgaa tgcttttatc gtaattatat ttttactatt
     3121 agctcaaata ttttattttt atatcattat acaattgcct aaattactaa aagaaccttt
     3181 ttcgcccgta ttttcagctt tcacattccc tttagtaatc tcagcaactg ctttaaagaa
     3241 cagtttgcct gtacttatgt ttccagacat ttggaaaggt cttttgttta tcgaagtgtt
     3301 attagccact gtaatagtac ttagagtctt tataggatat cttcacttct ttttaaaaaa
     3361 ggaaaaacaa gataaatttc ttcgtaatgc gtctcagtaa cactattacc aagaattaac
     3421 acgtatattt aataactaaa aagaggcagg taatcttgcc tctttttagt gtatatcaat
     3481 gcctctttga tttaatatga tcttatcaat gataccttct gactaaagct atagtaacta
     3541 tactagaacg tctagttcat aaacacgttt cactctaaac tttatttaaa taatatataa
     3601 gcagcaaacg ccaaggctaa tataccaata aaataattaa taaagatttt tattttagtc
     3661 tttgtaatag ttgacaaatt ttatcacctc taaatatcga taaattaaac accgttattt
     3721 ttcctttgcc tatcttttat catttcagaa ggtactccga aaataaacat cagaataaat
     3781 aatataattg gcaaatgtgg tatgttttct agcaaatacc atcttaaatt ccaatcaaac
     3841 accatatttg catacgccac aaaatattta ctaaataata atactacgca tattccagtt
     3901 agtgtatcta aaacctttgt caaatgtgtc atttatagcc atcctttata tatgctattt
     3961 tcacaaccat atgtatcatg tgttacaaaa actgtttaca gcaaatacat atttaccacg
     4021 ataaatataa tataccatag atctaacatt acattaaaaa caaattttaa aaatatcatt
     4081 attaaataga gcattgtatc aattacttac tagtgttatc attaccgtca ttattttctt
     4141 aatcaaaaat aataatgcac ctcgcttagt ttagtatttc tacaaaccta cgagatgcat
     4201 aaaaccccat tagtattttt tataatatag catttcaaat tatgaataaa ttagtacttg
     4261 cgtaattagt actccgccac cgaaaatcaa tacaaaagca actaccggcc atacaaatcg
     4321 gaaccaatgt gaatatcgca tgtttaacat ttgtagtgtg gccataacaa gtccagtcgg
     4381 cgctaagaat aacatcgcat attgaccaaa ttgatatgtc gtaacgatga cgaatcttgg
     4441 tatacctact gtatcagcta gtggtgcaaa gataggcatt gataataccg ctaagccaga
     4501 agatgatggc acgataaaac ctaaacagaa gaaaataaat agtaatacga tgataaataa
     4561 tggtccactc atatgttgaa ctaaagatga tgaaaagtgt aagattgtat ctgaaatcat
     4621 accttcattc aacactaaat taatacctcg agctaaacca ataattaaag atacacctac
     4681 taaacttgat gcaccattga caaatgcatc tacagttcct ttttctccca atccagattt
     4741 acctgtccca gcaataaaca ttattataat tgtaaatatt aaaaatgctg aagccataac
     4801 tgggaaccac caaccttgcg tcataactcc ccataccata attggaaatg gtagtacaaa
     4861 taatgtaagg attatcttct tacgcaaagt aaaatgggca ctatcgtcat cttttaatac
     4921 agaccattgc tgttcaaaag catctttgtc ttcataagaa tatgacgctt taggatcgtt
     4981 tttaattttt ttacagtacc aatataaata actaataaca aaaatcgcac cgacaataca
     5041 agcacctatt ctccaataca agccatccgt aaaagttgta ccagcggcat tagaggcaat
     5101 tacaaccgag aacgggttaa tagttgaaaa tgtactaccg acagagctgg caaggaatat
     5161 ggcaccaact gaaacgatag aatcgtatcc taacgctata aatataggga ctaaaatcgg
     5221 ataaaatgct acagcttctt cttcaatacc acataatgtc ccgccgataa tcattagtat
     5281 tgatacaaac acaattagca taaattcatg cccttttgtt ttcttcgtta aagctaacaa
     5341 tcccgattca aaagaaccac tggcttgaac tacgccaata agtcccccta gtacaagaat
     5401 gaataccatg atatcgaccg cttctatcgt accttcaacc atgctacttg ttatttgttc
     5461 tggtccagct ggatgttgct ttaatctttc ataagtattc ggaattgata ccggcttatt
     5521 aattgcacct gatttaaatt gttcaatctt aattttaacc cccattttgt ctagttcctg
     5581 ttgcgtaccc ggaacctttt tcacttggtt atgagggtta actatcttta gttcttggga
     5641 tgaaggttcg taagaaagtt tagaatatgc accagcagga ataacccatg ttgctataac
     5701 tgcaacaacc gttaaaatga ataaaatcat aaatgcacct ggcattttta atttaaatcg
     5761 ttttttcttt tcactttcat taattgtatt ttccacggtt tcatctcctt cgacatttaa
     5821 cctagcattt ctaccttaaa gattttataa atataaatta agaaagtgca ccccgcatca
     5881 aaatagaggc attattttca gggggtgcac ataaataata aaaatcatgc atttgacata
     5941 tagtaattga aaagcgtttc aattcaatta ctttttaatc acagtaccta ctttaccctc
     6001 taaggcagca tctaattcat ttaatgatgt tataagcaca cttccttttg gattgttttc
     6061 aataaatgat atggctgctt caatttttgg taacatactt ccttttgcaa attgattttc
     6121 gtctatatat cgttttaatt catcaacatt tgttgttttc aaaggctgtt ggttttcagt
     6181 gttaaaatta atatatacat aatcaattgc tgttaaaata atcaattgat cgcattgaat
     6241 attagcaccc aacaacgcac ttgttttatc tttgtctata actgcatcaa tacctttaaa
     6301 accatcatgt tgctctctaa ttactggtat acctccacca ccagcagcaa taacgagtgt
     6361 atcattttta ataagtgttt taatactctc taattcaata atagagatgg gttgtggtga
     6421 aggaacaacg cgtctatatc ctcttccagc atcttcaaca aatataaatc ctttttcttt
     6481 ttgaatttgt tcagcttctt ctttgttgta aaataaccca attggttttg aaggattgtt
     6541 aaatgccgga tcattttcat caacttcaac ttgtgtcact agtgttacca cttgtttatc
     6601 cattccaata gaatgcaatt cattttgtaa gctttcttgt aattgatagc cgatgtaagc
     6661 ttgactcatt gcgccacatt cagcaaatgg aaatgccgga ccttggttat gttctgcagc
     6721 atagttaagt cccaaattaa tgcttccaac ctgtggtcca ttaccatgac taataacaat
     6781 ctcatgtcct tttgttatta atcctactaa tgatttcgca gtatttttaa caagctcgag
     6841 ttgttcttga ggtgattttc ctaaagcatt accacctaat gctactacta ttttcgccat
     6901 catattcact tccttatatc atttaaaatt cacccaatgt agcaaccatg actgctttga
     6961 ttgtatgcat tctgttctca gcttcttgga atacaactga agctttactt tcgaatactt
     7021 catctgtaac ttccatttct cgaataccat atttttcaaa aatttgttga cctattttcg
     7081 tatcagcatt atggaaagat ggtaagcaat gctcaaaaat aacatttgga ttaccagttt
     7141 tatccattat ttctttattt acttgatatg gtttcaataa ttcaagtcgt tctttccata
     7201 cttcatcagg ttcacccatt gatacccaaa catcagtgta aattacatcc gaacctttta
     7261 caccttggtc aatatcatct gtgattaata tgttgccacc attttcagcg gcaatatttt
     7321 tacagcgatt taataattca tctgttggat ttaattcttt tggacaaact aaatggaagt
     7381 tcatacccat aatggcagca ccttgcatta atgcatttgc aacgttatta cgaccatctc
     7441 caacatatgt aaagttaata tctgcataat ctttttttaa gacttctttt gctgttaaga
     7501 aatcagcaag aacttgagta ggatgatctt catcagttaa accattccac actggtacgc
     7561 ctgaatactc agctaaagtt tctactgttc tttgtgaaaa accacggtat tcaatgccat
     7621 catacattcc accaagcaca cgtgcagtat ctttagttgt ttctttttta cccatttgtg
     7681 atccagttgg gcctaaataa gttacatttg caccttgatc atgcgctgca acttcaaatg
     7741 cacatcgcgt tcttgtagaa tctttttcaa ataacagtgc aatattttta ttttttaaca
     7801 taggcttttc agtgccaata tatttagcac gttttaaatc ctcggagagt gttaataaga
     7861 attctacctc ttgtcgtgaa aagtctaata aagttaaaaa acttctgttt cgtaaatttt
     7921 tcattaataa caactccttt tattttagtt acacctaaat tgtaagcgca tacaaaataa
     7981 acacaaccta ctattaaaat ttgtaatatt ttatcaataa ttaaatgaac attttattaa
     8041 tattaaattt aagtagtagg aaataattaa aataagtact acatttaaag tataactatt
     8101 tttcaagtag ttagaaaatt caattatcaa acaatttaat acaattgatt agagaataat
     8161 tctaacgtat cgtttttaat atataactcc cgcctacttt attagatact gtttctgtcc
     8221 aaaacttaaa aatgataagt tttgcttaaa taacactact aactgtttaa gtttatttaa
     8281 catagtttta gcttttattt aattccgaat cggtgtaata gcttatatac tttgggtaat
     8341 tcacgcaaag gagattttca tatgaaaaag aacatcatga ataaattagt tttatcaaca
     8401 gcattgttac ttttagaaac tacatcaaca caacttccta aaacaccaat cagtttttca
     8461 tctgaagcaa aagcctataa tatcagtgaa aacgagacta atatcaatga actaatcaaa
     8521 tattacactc agccgcattt ttcattatct ggaaaatggt tatggcaaaa gcccaatggt
     8581 agcattcatg caacattgca aacgtgggtt tggtatagtc atattcaagt gtttggatcc
     8641 gagagttggg gaaacattaa tcagttaaga aataaatacg ttgatatatt tggaactaaa
     8701 gatgaggaca cagttgaagg ttactggact tatgatgaaa catttactgg tggtgttacg
     8761 ccagcagcta cttcatctga taagccttat agactatttt taaaatatag tgataaacaa
     8821 caaactatca tcggtggaca tgaattttac aaaggaaata aaccagtatt aactttaaaa
     8881 gaattagatt tccgtattcg tcaaacatta ataaaaaata aaaagttata taacggagaa
     8941 tttaataaag gtcaaattaa gataactgct gatggaaata attacacgat tgatttaagt
     9001 aaaaagttaa aattaactga cacaaaccgt tatgttaaaa atcctcgtaa tgcagaaatt
     9061 gaagtcatac tcgaaaaatc taactaacct attacctttt gtaaatgcgg ataatttcaa
     9121 ttatctaatt aacccctttt tataattaaa cattccaaca atactcaaag gagaaattcg
     9181 aatgaacaat aacatcacga aaaaaattat tttatcaaca acattgttac tattaggtac
     9241 agcatctaca caatttccta atacacctat caattcttca tctgaagcga aagcttatta
     9301 tataaatcaa aacgaaacta acgttaatga gttaactaaa tattactcgc aaaaatattt
     9361 aaccttctct aacagtacgt tatggcaaaa agataacggt acgattcatg caacgttgtt
     9421 acagttttct tggtatagtc atattcaagt ttatggacct gaaagttggg gcaatatcaa
     9481 ccaattaaga aataaaagcg ttgatatttt tggcataaaa gaccaagaaa ccattgattc
     9541 ttttgcatta tctcaagaaa cgtttactgg tggtgttact cctgcagcaa catctaacga
     9601 taaacactat aaactgaatg taacatataa agataaagca gaaacgttta ctggcggatt
     9661 tccagtttat gaaggcaata agcctgtttt aactttaaaa gaattagatt ttcgtattcg
     9721 tcaaacatta attaaaagta aaaaattata taataattct tataataaag gacaaattaa
     9781 aataacaggt gcagacaata actacacaat agatttaagt aaaaggttgc catcaactga
     9841 tgcaaataga tatgttaaaa aacctcaaaa tgcaaaaatt gaagttatcc tcgaaaaatc
     9901 aaactaacaa t
//
LOCUS       DAKTLH010000094         9750 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.094, whole genome shotgun sequence.
ACCESSION   DAKTLH010000094 DAKTLH010000000
VERSION     DAKTLH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9750
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.094"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            94..1218
                     /locus_tag="O0394_001891"
     CDS             94..1218
                     /locus_tag="O0394_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCY0499963.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRILKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVKRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNTGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFG
                     QTINNLKEKELIVEKNDVIALTNRGKVIGNEVFEAFLIND"
     gene            1318..2295
                     /gene="hrcA"
                     /locus_tag="O0394_001892"
     CDS             1318..2295
                     /gene="hrcA"
                     /locus_tag="O0394_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HCY0499964.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            2327..2953
                     /gene="grpE"
                     /locus_tag="O0394_001893"
     CDS             2327..2953
                     /gene="grpE"
                     /locus_tag="O0394_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCY0499965.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            3022..4854
                     /gene="dnaK"
                     /locus_tag="O0394_001894"
     CDS             3022..4854
                     /gene="dnaK"
                     /locus_tag="O0394_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCY0499966.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            4990..6129
                     /gene="dnaJ"
                     /locus_tag="O0394_001895"
     CDS             4990..6129
                     /gene="dnaJ"
                     /locus_tag="O0394_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCY0499967.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            6133..7071
                     /gene="prmA"
                     /locus_tag="O0394_001896"
     CDS             6133..7071
                     /gene="prmA"
                     /locus_tag="O0394_001896"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCY0499968.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            7073..7825
                     /locus_tag="O0394_001897"
     CDS             7073..7825
                     /locus_tag="O0394_001897"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCY0499969.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIEVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            7832..9178
                     /gene="mtaB"
                     /locus_tag="O0394_001898"
     CDS             7832..9178
                     /gene="mtaB"
                     /locus_tag="O0394_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HCY0499970.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            9471..9647
                     /locus_tag="O0394_001899"
     CDS             9471..9647
                     /locus_tag="O0394_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCY0499971.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
BASE COUNT         3702 a         1339 c         1880 g         2829 t
ORIGIN      
        1 gattaaacgt taaagttaaa agtaagaata attgcagata gtccaacagg atatagccga
       61 ttggataaaa agtctgagaa gcggggcatt aaaatgacgg tacaaagtgc atatatacat
      121 attccatttt gtgtaagaat atgtacatat tgtgatttca ataaatattt tatacagaat
      181 caacctgtag atgagtactt agatgcacta atcacagaaa tgtctacagc aaaatatagg
      241 atcttaaaga ccatgtatgt aggtggcggc acaccaacgg ccctttctat taatcagttg
      301 gaaagattac ttaaagcaat acgtgatacg tttacaatca caggcgagta tacatttgaa
      361 gcaaatcctg atgagttaac taaagagaaa gtccaactat tagagaaata tggagtaaaa
      421 aggatttcaa tgggcgttca aacattcaag ccggagttat tgtctgtttt aggtagaacg
      481 cacaatactg aagatattta cacttcggtg ttaaatgcta aaaacacagg tattaaatca
      541 atcagtttag atttaatgta tcatttaccg aaacagacga ttgaagattt tgaacaaagt
      601 ttagatctag ctttagatat ggatattcaa catatttcga gttacggctt aatacttgaa
      661 cctaaaaccc aattttataa tatgtataga aaaggcttgc tcaaacttcc taatgaggat
      721 ttaggtgctg acatgtatca gttgctgatg tctaagatag aacaatctcc tttccatcaa
      781 tacgaaatat ctaattttgc attagatggc catgaatcag aacataataa ggtttactgg
      841 tttaatgagg aatattatgg atttggagca ggtgcaagtg gttatgtaga tggtgtgcgt
      901 tatacgaata tcaatccagt gaatcattat atcaaagcta taaataaaga aagtaaagca
      961 attttagtat caaataaacc ttctttgact gagagaatgg aagaagaaat gtttcttggg
     1021 ttgcgtttaa atgaaggtgt gagtagtagt aggttcaaaa agaagtttga ccaatctatt
     1081 gaaagtgtct ttggtcaaac aataaataat ttaaaagaga aggaattaat tgtagaaaag
     1141 aacgatgtga ttgcacttac aaatagaggg aaagtcatag gtaatgaggt ttttgaagct
     1201 ttcctaataa atgattaaaa aaattgaaat ttcgagtctt taacattgac ttactttgac
     1261 caatttgata aattataatt agcacttgag ataagtgagt gctaatgagg tgaaaacatg
     1321 attacagata ggcaattgag tatattaaac gcaattgttg aggattatgt tgattttgga
     1381 caacccgttg gttctaaaac actaattgag cgacataact tgaatgttag tcctgctaca
     1441 attagaaatg agatgaaaca gcttgaagat ttaaactata tcgagaagac acatagttct
     1501 tcagggcgtt cgccatcaca attaggtttt aggtattatg tcaatcgttt acttgaacaa
     1561 acatctcatc aaaaaacaaa taaattaaga cgattaaatc aattgttagt tgagaatcaa
     1621 tatgatgtat catcagcatt gacatatttt gcagatgaat tatcaaatat atctcaatat
     1681 acaactttag ttgttcatcc taatcataaa caagatatta tcaataatgt acacttgatt
     1741 cgtgctaatc ctaatttagt tataatggtt atcgtatttt catcaggtca tgttgagcat
     1801 gtacatcttg cttcagacat accgtttaat aatgacaaac taaatacaat atcaaatttt
     1861 gttacaaaca aactaactga gtttaatcag aatttacaag atgatattgt ctcttttgtt
     1921 caatcagagc aggaagaaat ttttatcaat aaattgttaa atacgatgaa taatcatatt
     1981 tctaatcaaa gtaatagcat ttatatgggt ggaaaggtta aacttattga tgcattgaat
     2041 gaaagtaatg tatcttcaat tcagcccatt ctacaatata tagaatcaaa tagaattgca
     2101 gaattattac aagatatatc ttcaccaaat attaatgtaa aaattggtaa tgaaattgat
     2161 gacagcttaa gtgatatttc aattgtaaca agtcaatatc attttgatga aacgttaaaa
     2221 ggtcaaatcg ctgtgattgg acctacagct atgcattatc aaaatgtcat tcaattatta
     2281 aatagaattt ggtaatgatg gattagaaag tattggagga cagaaaatga caaataaaga
     2341 cgaatcagtt gaaaaaaaca ctgaatcaac agttgaagaa acaaacgtca aacaaaatat
     2401 tgatgattca gttgaacaag ctgaagaaag taaaggtcat ttacaagatg aagcaataga
     2461 agaaacgtct gacgagaatg ttattgaaga aatagatcca aaagatcaaa aaattaatga
     2521 acttcaacaa ttagcagatg aaaacgaaga gaaatattta aggctctacg ctgagtttga
     2581 aaattataag cgtagaattc aaaaagaaaa tgaaataaac aaaacatatc aagcacaacg
     2641 tgtgttaaca gatattttac cagcaataga caatatagaa cgtgcacttc aaattgaagg
     2701 tgatgatgag acttttaaat ctcttcaaaa aggtgtacaa atggtgcatg aaagtttgat
     2761 taacgcacta aaagataatg gtcttgaagt tattaaaact gaaggtgaag catttgatcc
     2821 aaatattcac caagctgtag ttcaagatga taaccctgat tttgaatctg gcgaaatcac
     2881 tcaagaacta caaaaaggat acaagcttaa agatagagta ttaagaccat caatggtcaa
     2941 agtaaaccaa taacttaaat ttggcgaaaa gacattgttt aaaattaaat taatttaatg
     3001 attaaatgga ggaattttat tatgagtaaa attattggta tagacttagg tacaacaaat
     3061 tcatgtgtaa cagtattaga aggcgatgag ccaaaagtaa ttcaaaaccc tgaaggttca
     3121 cgtacaacac catctgttgt agctttcaaa aatggagaaa ctcaagttgg tgaagtagca
     3181 aaacgtcaag ctattacaaa cccaaacact gttcaatcta ttaaacgtca tatgggtact
     3241 gattataaag tagatattga aggtaaatca tacacaccac aagaaatctc agctatgatt
     3301 ttacaaaact taaaaaatac agctgaaagc tatttaggtg agaaagttga caaagctgta
     3361 attacagtac ctgcatactt taacgatgct gaacgtcaag caactaaaga tgctggtaaa
     3421 attgctggtt tagaagttga gcgtatcatt aatgaaccaa cagctgcagc attagcatat
     3481 ggtttagaca aaactgataa agatgaaaaa gttcttgttt ttgacttagg tggcggtaca
     3541 tttgacgtat ctatcctaga attaggtgac ggtgtattcg aagtactatc aacagccggt
     3601 gacaacaaac ttggcggtga tgattttgac caagtaatta ttgactacct agttgcagaa
     3661 ttcaaaaaag aaaatggcgt agacttatct caagataaaa tggcattaca acgtttgaaa
     3721 gatgctgctg aaaaagctaa aaaagactta tcaggtgtat cacaaactca aatctcatta
     3781 ccatttatct cagctggtga aaacggtcca ttacacttag aagtaaactt aactcgttct
     3841 aaatttgaag aattatcaga ttcattaatt agaagaacaa tggaacctac acgccaagca
     3901 atgaaagacg ctggcttaac aaactcagat atcgatgaag ttatcttagt tggtggatca
     3961 actcgtattc cagcagtaca agaagctgtc aaaaaagaaa tcggtaaaga gcctaacaaa
     4021 ggagtaaacc cggacgaagt agtggcaatg ggagctgcaa tccaaggtgg cgttatcaca
     4081 ggtgacgtta aagacgtagt attattagac gtaacaccac tatctttagg tattgaaatt
     4141 ttaggtggac gtatgaatac gttaattgaa cgtaacacta cgattcctac atctaaatct
     4201 caaatctatt caacagcagt agataatcaa ccatcagtag atgtacacgt attacaaggt
     4261 gaacgtccaa tggctgcgga taataaaaca cttggtagat tccaattgac tgatattcca
     4321 ccagctgaac gtggtaaacc tcaaattgaa gtaacgtttg atatcgataa aaacggtatt
     4381 gtaaatgtaa ctgcaaaaga cttaggtaca aataaagaac aaagaattac aattcaatca
     4441 agttcttcat tatcagacga agaaatcgac cgtatggtaa aagatgctga agttaacgct
     4501 gaagcagata aaaaacgtcg tgaagaagta gacttaagaa acgaagctga cagtctagta
     4561 ttccaagttg aaaaaacttt aactgattta ggcgaaaata tcggtgaaga agataaaaaa
     4621 tctgctgaag agaaaaaaga cgctcttaaa actgctttag aaggtcaaga tatagaagat
     4681 attaaatcta aaaaagaaga acttgaaaaa gtgattcaag aattatcagc aaaagtatat
     4741 gagcaagcgg ctcaacagca acaacaagca caaggtgcta atgctggtca aaacaatgat
     4801 agtactgtag aagatgctga atttaaagaa gtaaaagacg acgacaaaaa ataattaaac
     4861 aatacaatta gaatgttgtc atgatctgaa aagtcaaagt caatagaaca ttggctttgg
     4921 ctttttattt ggatagtatg acatattacg ttaaactata tgagttaatt aagaaaagga
     4981 gagatagctg tggccaaaag agattattat gaagttttag gcattagtaa ggatgcttca
     5041 aaagatgaaa tcaaaaaagc gtatcgaaag ctttcgaaaa aatatcatcc agatattaac
     5101 aaagaagaag gtgcagatga aaagtttaaa gaaatttctg aagcctatga agttttaagt
     5161 gatgataata aacgcgcaag ctatgatcaa tttggacatg atggtccaca aggttttggt
     5221 ggacaaggat tcaatggctc tgactttggc ggttttagtg gcttcggtgg cggcggtttt
     5281 gaagatatct ttagctcttt cttcggtggc ggcagacaaa gagatccaaa tgcaccgcaa
     5341 aaaggtgatg atcttcagta tacaatgaca ttaacatttg aagaagcggt atttggtaca
     5401 acaaaagaaa tttcaatccg taaagatgta acatgcgaaa catgtcatgg tgatggtgca
     5461 aagcctggaa caagtaaaaa gacttgtagt tactgtaatg gcgctggtca tgtagctgta
     5521 gaacaaaaca caattttagg tagagttcgt actgaacaag tttgtcctaa atgtaatgga
     5581 agtggtcaag aatttgaaga agcttgtcca acatgtcacg gaaaaggtac tgaaaataaa
     5641 acagttaaat tagaagtgaa agtacctgaa ggtgtcgaca atgaacaaca aattagatta
     5701 gctggtgaag gctctccagg cgtgaatgga ggacctgctg gtgatttata cgtagtattt
     5761 agagttaaac catctgaaac atttaaacgt gatggtgatg atatttatta caaattaaat
     5821 gttagcttcc cacaagcagc tttaggcgat gaaataaaaa taccaacatt aaataatgaa
     5881 gttatgttaa caatccctgc tggtacacaa acaggtaagc aattccgctt aaaagaaaag
     5941 ggtataaaaa atgttcatgg ctatggatac ggagatttat atgttgatat taaagtagta
     6001 acgcctacta aattaacaga tagacaaaaa gaactaatga aagaatttgc ccaattaaat
     6061 ggtgaagaaa ttaatgaaca accttcgaat tttaaagata gagcaaaaag attctttaag
     6121 ggagaataaa gtatgaactg gacagagctt tcaattatta ttaatcatga agcagtagaa
     6181 ttggctacca atatacttga aaatcatgga tcaaatggtg tcgtgataga agattcagat
     6241 gatttaatta accaaccaga agataaatac ggtgaaattt acgctttgaa aaaagaggat
     6301 tatccagata agggagtaag attgaaagcc tattttaatg aaatgactta tgatgataag
     6361 ttgcgacagc aaattaaaga tgagttatta aatttagatg aacttgatca acataacgtt
     6421 caattcagtg agcaaattat tgcagagacg gattgggaaa atgaatggaa aaactatttc
     6481 catccattcc gagcgtcgaa gaagttcaca atagttccta gttgggaaac atatgctaaa
     6541 gaagcggatg aagagctttg cattgagctc gacccaggta tggcttttgg aacaggtgat
     6601 catccgacta caagtatgtg tttgaaggca atagaaacat atgtattgcc acagcattca
     6661 gtaattgatg ttggtactgg ctcaggtata ttaagtattg caagtcatct aatcggtgta
     6721 aaacgtatta aagcgttgga tattgatgaa atggcagtga gtgtagctaa agaaaacttc
     6781 agaagaaatc attgtgaaac gttaattgaa gctgttccag gtaacttatt gaaagacgaa
     6841 acagaaaaat ttgatattgt aatagcaaat attttagcgc atattattga tgaaatgatt
     6901 gaagatgctt ataatactct aaatgaaggc ggttatttta ttacttctgg tattataaaa
     6961 gagaagtatg aaggtataca gtcacatatg gagcgtgtag gttttaaaat tatttcagaa
     7021 caacatgaca acggttgggt ttgtcttgtt ggccagaaag tgagtgaata atgtgcaacg
     7081 ttatttcata gaccaaaacg ctgatgtaag tcagcgtttt tttattacaa aaaaagaaga
     7141 tattcatcat ataacaaatg taatgagata tgatgttggt agtaaaatta ttttaacttt
     7201 taatgatcaa acagtttatt tatgtgaaat tgctgacata acaagtgata aaattgaagt
     7261 tgatttaaaa gaaaaacaaa atattaatac agaattgcca gttgatgtta cgatttgcag
     7321 tggactaatc aaagctgaca aatatgagtg gttgctacaa aaagctactg aattgggtgc
     7381 ttcatcattt atagctgtga gcatggaacg ttcaattgtt aaattaaacg aggcaaaagt
     7441 tgccaaaaag atagagcgtt ggcaaaaaat aattaaagaa gctgctgaac aaagctatcg
     7501 tttagtgatt ccatctattc aattcgagtc gaatttaaaa ttaatttgtg atactattga
     7561 taattatgac tatattctta ttgcatatga agaggaagca aaagatggtg agttaagcaa
     7621 tttcaagcaa actttacaac aattcaatgc tcaggataaa gtgttgatga tttttggtcc
     7681 tgagggtggt ttgtcagaaa atgaaatttc attatttagt aatactagta cagttgttgg
     7741 tcttgggccg agaattttac gggcagaaac tgcgccattg tatgcgttaa gtgcaataag
     7801 ttatgaaaaa gaattaatgg ggtgaatatt tatgtcaaca gttgcgtttc acacattagg
     7861 ttgtaaagta aaccattatg aaactgaagc aatctggcaa ttatttaaag aagcaaacta
     7921 tgagcgcgtt gactttgaag cgaatgctga tgtatttgtt attaatactt gtacagtaac
     7981 gaatacgggt gataaaaaaa gtcgtcaaat aattagacgt gcaataagac aaaatcctga
     8041 tgctgtaatc tgtgtaacag gttgttatgc gcaaacttca tcagctgaaa ttatggaaat
     8101 tcctggtgtc gatgtagtag ttggtacaca agatagacat aaactattag gttacattga
     8161 cgaattccgt aaagaacgcc aaccaattaa tggtgttgga aatatcatga aaaatcgtaa
     8221 atatgaagaa ttagatgtcc cttattttac agatagaaca cgtgcgtcat taaaaattca
     8281 agaaggttgt aacaacttct gcacattctg tattattcca tgggctcgtg gcttaatgcg
     8341 ttcaagagat ccggaaaaag tagttgaaca agcgacgcaa ctagtgaatt caggatataa
     8401 ggaaattgta ttgacgggaa ttcatacagg tggatatggt caagatttaa aagattataa
     8461 cttggcccaa ttattacgtg atcttgaaac gattaatgga ttagaacgaa ttcgaatttc
     8521 ttcaattgaa gcaagtcaac ttacagatga agtaattgac gttttagaac gttcaacaaa
     8581 agttgtgcgt catttgcata ttccattaca atctggttca gatacagtat taaaacgtat
     8641 gagacgtaag tatacaatgg atagattttc agaacgatta acaaaattgc ataaagcttt
     8701 accagacttg gcagttacga gtgatgtaat tgttggtttc ccaggtgaaa ctgaagctga
     8761 gttccaagaa acatatgatt ttatcgtaaa gcataagttc tctgaactgc atgttttccc
     8821 ttattctcct agaattggca cgccagctgc aagaatggat gaccaaattg atgaagaaat
     8881 taaaaatgaa cgggtgcata agttaattac gctaagcaat caactcggaa agttatatgc
     8941 gtctaaattt gatcaagatg tgcttgaagt aattcctgag gaacagggtg acacagaagg
     9001 tacattagtt ggatatgcag ataattatat gaaagtacaa tttgaaggtg acgaatcact
     9061 cataggtcaa attgtaaaag ttaaaattac gcaagcaaat tatccattaa atgaagggca
     9121 agcaattaaa gttgttgatt tcgcaacaaa taaatctgat agagaagttt tagtttaagt
     9181 attccttgat tcataccata tggttccgta ttaaagatat aggttttgta acgttgtttt
     9241 aacggcttca caatttacta tataagcaac tcttctcatt aaataaatta ttaagagatt
     9301 tttagaacaa aatctaaaag tctttgtgat tttgatgtga aatatgtttc aaataaaaaa
     9361 caatttacta attgaccata aattacagat atattatact tataaatgca tagttttact
     9421 gtgcaattga ctataaagtt ccgttgatat ttggagggag ggaaatacag atgtctaaaa
     9481 cagtagtacg taaaaatgaa tcacttgaag atgcgttacg tagatttaaa cgttcagttt
     9541 ctaaaagtgg aacaatccaa gaagtacgta aacgtgaatt ttacgaaaaa ccaagcgtaa
     9601 aacgtaaaaa gaaatcagaa gctgcacgta aacgtaaatt caaataatta atacctctgt
     9661 tgactccctc aacacgaata ttaattatat aaaacaaaca tcacaagtta gtgtctgaca
     9721 ctaatatgtg atgttttttt gttgtcaatt
//
LOCUS       DAKTLH010000095         9632 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.095, whole genome shotgun sequence.
ACCESSION   DAKTLH010000095 DAKTLH010000000
VERSION     DAKTLH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.095"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            56..3037
                     /locus_tag="O0394_001900"
     CDS             56..3037
                     /locus_tag="O0394_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCY0499972.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFVDITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFSKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            complement(3149..4087)
                     /gene="manA"
                     /locus_tag="O0394_001901"
     CDS             complement(3149..4087)
                     /gene="manA"
                     /locus_tag="O0394_001901"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCY0499973.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(4101..6053)
                     /locus_tag="O0394_001902"
     CDS             complement(4101..6053)
                     /locus_tag="O0394_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HCY0499974.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSL
                     VTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDNTAKHSHLNKPSHVF
                     DQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMGSTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWDSLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            complement(6130..8004)
                     /locus_tag="O0394_001903"
     CDS             complement(6130..8004)
                     /locus_tag="O0394_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCY0499975.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAKHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDSKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TTIKVAPLFSEDDRHKIRHVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            complement(8244..8702)
                     /locus_tag="O0394_001904"
     CDS             complement(8244..8702)
                     /locus_tag="O0394_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499976.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKSTHK
                     KTETEENDQQDDSADKTDKNQNNGNNVTNDNQPSSPSNVPNNHSTVPSNTQPAQPKDS
                     NSNKNNTHGGGSQSSTNNQNNQQQNTPPANKSNNTNTPSKQTPQAQSPKN"
     gene            8960..9487
                     /gene="isaB"
                     /locus_tag="O0394_001905"
     CDS             8960..9487
                     /gene="isaB"
                     /locus_tag="O0394_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HCY0499977.1"
                     /translation="MNKTSKVCVAATLALGTLIGVTVVENSAPTSKQAQAAITPYYTY
                     NGYIGNNANFILDKNFINAIKYDNVKFNGIKLAKTNTIKKVEKYDQTFKGVSAKGNEA
                     SQLQFVVKNNISLKDIQKAYGKDLKKENGKTKEADSGIFYYQNAKKTLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
BASE COUNT         3236 a         1524 c         1735 g         3137 t
ORIGIN      
        1 taatttacaa aaaatatata aaaaatatga ataattccta cataggagtg tgacaatgaa
       61 gaacgcattt aaattattta aaatggatct gaagaaagta gctaagacgc cagctgtgtg
      121 gattatctta gcaggcttag ctattttgcc atcgttctac gcttggttta acttatgggc
      181 aatgtgggat ccatatggca acacgggaca catcaaggtc gcagtcgtta atgaagataa
      241 aggcgacaca atcagaggga aaaaagttaa tgtcggtaat acgatggtta atacactcaa
      301 gaaaaataaa agttttgatt ggcagtttgt aagtagagag aaagctgatc atgagataaa
      361 aatgggtaaa tattttgcag gtatttacat cccatctaag tttacacatg aaattacagg
      421 gacactacgt aagcagcctc aaaaagcaga tgtagaattt aaggtgaatc agaagattaa
      481 cgctgttgcg tctaagctaa cagatactgg ttcgtcagtt gtcgttgaaa aagcgaatga
      541 acaatttaat aaaacagtaa ctcgagcatt attagaagaa gctaacaaag caggtttaac
      601 tattgaagaa aatgtgccga caattaacaa gataaaaaat gcggtatatt cagcagataa
      661 agctttacct aagattaatg actttgcgaa taaaattgta tatttgaata accaccaagc
      721 ggatttagat aaatatgcca atgattttag aaaactagga aattataaag gtgatatttt
      781 agatgctcag aaaaaattaa acgaagtcaa tggtgctatt ccgcaactta atgaaaaggc
      841 taagttgata ttagctttaa ataattatat gccgaaaatt gaaaaagcgt taaattttgc
      901 agctgatgac gtgccagcgc agttccctaa aattaatcaa ggacttaaca ttgcgagtca
      961 aggtattgat caagctaatg gacagttaaa tgatgccaaa ggcttcgtca cacaagttag
     1021 aagtagagtc ggtgattatc aagatgcaat tcgacgcgcg caagatttaa atcgaagaaa
     1081 ccagcaacag attcctcaaa atagcgcggc gaacaacgaa acatcaaata gtgcacctgc
     1141 agctggtaat ggtgtagcat caacgccacc aagtgcacca agtggcgata ctgcaccaaa
     1201 taataatgtt acgcaaaata ccgcaccaaa tagtaataat gcgcctgtat cgactacacc
     1261 acaaagtaca agcgggaaaa aagatggtca aagttttgta gatataacaa caacacaagt
     1321 cagcacagct aacgagaaca cacaaaacat tacagataaa gatgttaaat caatggaagc
     1381 ggcattaacg ggctctttat tatcattatc aaataattta gatacccaag cgaaagccgc
     1441 acaaaaagat agtcaggcat tacgtaatat ttcgtatggg attttagcat cggacaagcc
     1501 ttctgatttt agagagtctt tagataatgt taagtccggt ttagaataca caacgcaata
     1561 taatcaacaa tttatcgata cattaaaaga gattgagaag aatgaaaatg ttgatttatc
     1621 aaaagaaatt gataaggtaa aagcagctaa taatcgaatt aatgaatcat taaggttagt
     1681 taatcaatta agcaatgcat taaagaatgg tagttcagga actgctgaag ctactaaatt
     1741 actagatcaa ctttcaaaac tagattcatc attatcatca tttagagatt atgttaaaaa
     1801 agatcttaac agctctttag tatcaatatc acaacgtatt atggatgaat tgaacaaagg
     1861 gcaaactgca ttatccaatg ttcagtctaa attaaataca attgatcaag tcattaacag
     1921 tggacaagct attttaaaaa atggtaaaac acgtattgat cgcttacaaa cagtattacc
     1981 aagtattgaa caacaataca ttagtgctgt taaaaatgct caagcaaact tctcgaaagt
     2041 gaaaagtgat gtagctaaag ctgctaactt tgtgcgcaat gacttaccac agttagaaca
     2101 gcgattaact aatgcgacag caagtgtgaa taaaaattta ccaacgttat taaatggtta
     2161 tgatcaagcg gtaggattac taaataaaaa tcagccacaa gcgaaaaagg ctttatcaga
     2221 tttagctgat ttttctcaaa ataaattgcc tgatgttgaa aaagatttga aaaaagcgaa
     2281 taaaattttc aagaaattag acaaagatga tgcagtcgac aaattaatcg acacacttaa
     2341 gaatgatttg aaaaagcaag cgggtattat tgcaaatcct attaataaga agactgttga
     2401 tgttttccca gttaaggatt atggttcagg tatgacacca ttctatactg cactgtcagt
     2461 atgggtaggt gcactcttga tggtaagttt attaacggtt gataataaac ataagagtct
     2521 agagtcagtc ttaacgacaa gacaagtgtt cttaggtaag gcaggattct ttataatgct
     2581 tggtatgttg caagcactca ttgtatcggt tggagatttg ttaatcctaa aagcaggagt
     2641 tgagtcacct gtattatttg tacttataac gattttctgt tcgattattt tcaactcaat
     2701 cgtatatacg tgcgtatcat tacttggtaa cccaggtaaa gccattgcaa tcgtattgct
     2761 tgtattacaa attgcaggtg gtgggggaac attcccaatt caaactacgc cacaattttt
     2821 ccaaaacatt tcgccatact taccatttac gtatgcaatt gattcattac gtgaaacagt
     2881 aggcggtatt gttccggaaa tcctaattac aaaattaatt atattaacgt tatttggtat
     2941 aggattcttc gttgtaggtt taattttaaa acctgtaaca gatccattga tgaagcgcgt
     3001 atctgaaaaa gttgaccaaa gtaacgttac agaataaaaa ttaaatccac acattagggt
     3061 tatagctcct taatgtgtgg atttttatgt ttttagacag aagagatagt aatttctgtc
     3121 ttttatggga cggttgttat cattgctatt atccaggatg acttactata gcactaatat
     3181 taccgacaaa gtgaatatcc tcgtcttccg tagttaaaat aaagctagaa cctttttgga
     3241 tgtcatagtg cttatcgttt actgttaaag taccagtacc atcgataatt gtaactaagc
     3301 aataagcatg tggtttattg aattttaaat ctccatgaat atcccattta tatactgcaa
     3361 aatattgatt atctacaaat tgagttacag tgtgtgtgtc gatgtgagtt gttataggag
     3421 tagtatttgg ttcatgattg cctaattcaa tcacatcttt actttgctct aagtgcaaat
     3481 cacgcaattg accattttga tctcgtctat catagtcata aatacggtat gtcgtatcgg
     3541 aggattgttg tgtctctaaa attaaaatac ccgaaccaat ggcatggaca gtgccagcag
     3601 gaacataata aaagtcaccg ggcttaacag gtatacgttt gaaaagactg tcaaattcat
     3661 gattatcaat catgtctatt aacgtctgtt tattatgtgc atgtacgcca tatataattt
     3721 cagcacctgg ggctgcatct aaaatatacc aacattctgt tttacctagt tcgccttcgt
     3781 gttttaaagc gtagtcatca tctgggtgaa cttgaacaga taatttatca ttggcatcta
     3841 atactttagt tagcagaggg aaactatctc gtgaatcatt atcgaataat tcacgatgtt
     3901 gtgaccaaag ttgatctagg gtcatatcct tgtatggacc attgataatt gtattaggac
     3961 catttggatg tgcagaaatt gcccagcatt caccagttgt ttcattaggg atatcatagt
     4021 taaatgcttt taatgcatga ccgccccaaa ttctgtcttt aaaaacgggt tgtaaaaata
     4081 atgccatagt taaaactcct ctatattttc attaataagt tataaatttc tgtagtactg
     4141 tttgcattaa ttagtgattg gcgtgtctca tcattcatta acgctttaga taagcgctga
     4201 agtattttta aatgtgtatc ctgactgttg tttggtacgg caattaagaa tatcaattga
     4261 ggtagactac catctagact gtcccattta acaccatgat tatttttcat aacagctaca
     4321 atcggttgtt ttacaacatc agactttgca tgtggaatgg ccacgttcat gccaatagct
     4381 gtcgtagacc ccatttcacg ttctagtatt gcatttttta aatgcgatgt gtgctctaca
     4441 taacggcaaa ttttaagttt atgaatcaac atatcaattg cttcgtttcg agacatgtcg
     4501 tgatcagtaa ttatcatagt ttgttgatca aaaacatgag aaggtttatt gagatgtgaa
     4561 tgtttcgcgg tgttatctac attgtcaacc tctgtatcat gttgtgtaat atctgtatca
     4621 tgaagttgcg tgtgttgcgc tggtgcatct actgctataa ctggtgtatt gcgttttaat
     4681 aatagtacag tagtcattgt gacaagacta cctactatca ctgcaaagat aaaccataat
     4741 acatgatcaa taccacctaa tacagccacg attggacctc catgtgcgac tctatcgccg
     4801 acaccaccaa tggctgcaat gactgatgca atcattgcac caatgatgtt tgcaggtata
     4861 atgcgcaatg gatcttgggc tgcgaaagga atagcacctt cagtaatacc aaatagtccc
     4921 atagtgaagg aagccttacc catttctctt tcggaatgat tgaatttata cttttgaaca
     4981 aacgttgcta aacctaaacc gattggtggt gtacatacag caactgcgac catacccata
     5041 acggcgtaat taccttcagc aataagtgct gagccaaata aaaatgctac cttgtttact
     5101 ggaccgccca tatcgaaggc aatcatcgca cctataatca tcgcaagtat aataatatta
     5161 gcaccttgca tactttttaa ccaggttgtt aatgcctcaa aaatattaga aattggtgca
     5221 ccgattaaaa atataaatat caatcctaca acgaccgatg aaataatggg aataataatg
     5281 ataggcataa ttggtgccat tgcttttgga actttaatat ctttaatcca ctttgcgata
     5341 taacctgcta agaaaccagc aacaatacca cctaaaaatc ctgcgcctgc atcactgcca
     5401 taaaaactac cgtcagcagc gatagcgccg ccaatcatac caggaacaag accgggcttg
     5461 tcagcgatac taacagcgat atatccagct agtattggaa ccataaattt aaaggctaaa
     5521 ctaccaatgt tttcaatgga tttccaaaat gaatcatctg ggatgactaa tccttttgat
     5581 gtcgtttcac cgcctagagt cagcgcgatg gcgataagga gtccaccaac tacgataaaa
     5641 ggaaccataa acgatacacc gttcattaaa tgttgataca ccatttgaat accactttta
     5701 gacttaccgc gatctttcga atgataattt gtttcagatt gataaatagg cgcatcttga
     5761 ttaatgatac gttgaattag acctctcgga ttatgaatcc cttcgcgaac attttcatta
     5821 atcaaccgtt taccaacaaa tcgggacaga tcaacttgtt tatcagctgc aattatgaca
     5881 ccgtcagctt cttcgatgtc ttgcgtagtt aaaacatttt cagcaccaac accgccctgt
     5941 gtctctactt taatatccac acccatttct tttgctacct gctcaagctt ttcttgagcc
     6001 atatatgtat gtgcaatgcc atttgggcat gaggtaatag ctacaatttt cataaaatca
     6061 tctccttttc tatattgtaa gcgtattctc gatactaaaa aaaagaataa ttaccgttac
     6121 tagtggcaat tattcttgta agtattcaaa taactgttgc tttaaactat gatcatctaa
     6181 actacataaa tggttcactg aatcatcatc caagttagca attaattgca tcatttgttt
     6241 tgtaaaagct ttgtctttat gcgaaatcgc taagaaaaag acaagtttga catcgtgttg
     6301 tcgccaagga aaaacatctt ttgtgcgaaa aataagcaca tgtgattgta aaactttttc
     6361 aggatctcca tgaggaatcg ccataaaatt acctatgtat gtagaagatg atttctcacg
     6421 ctctaaagct gattcgatat atccttctac aatcgcatga tgtgcttgta atattttttg
     6481 agcttcttca aaaatttgca cagtatgccg tgatttttgt tcagtattta cgacaaggaa
     6541 attgacagtg tccatatgat gatgtgcttg aaccggattt tgcttttgct tcacaacgtg
     6601 tctgattttg tgacgatcat cttcagaaaa taatggtgca accttgatag tcgtcaggtg
     6661 cttaggaagt atgtttagcg tttgtttagg aatatcatgg gtcgttatta ataaatctac
     6721 attgtcaaag tgatagtgtg ttatattttc tagtttaatc gtatttatca ctgacaactc
     6781 ttcggataag ttatttattt tagtttctaa aaaattcgac acacctagac cataataaca
     6841 agcaatgact acatttaatt gtgttttggt acgacgctcg atggcagctt gaaaatgaat
     6901 tgttaaaaat gcaatttcat cttcgctcat ctctatatca gtatcaattg ctaatttatc
     6961 aatcgcttca aaaagtgtgt taaacacaaa gggatagagt tttttaatct ctataactaa
     7021 aggattgttt aaataaatgt tttgagtgat acgtaaatat gctttactaa aatgattata
     7081 taaattttgt tgtaaaatcg aatcttcatt gaaaggtaca tgaatacgtt gctgcatcaa
     7141 ttcgattaag cgatcaatat aactttgtat aaatatacgt tctatgccaa tatcgagttt
     7201 attaaaatga taagcaataa agaatgaaaa catattgatt actttttcgt tcaagtcata
     7261 acctaatctt tcgttgattt gcttaatgca agattgagat atcaattttg aatcgtggtt
     7321 taacgtgtct agctcactta aagattgctt acgtttaatg attaaaatga gatgaattaa
     7381 aagctgttgt atttgaatat cagttgtttc aatactatgt tgttgaagtg tctcttgtat
     7441 aatatgcgaa atcatccttt ggtgtgaatc aggtaattca tttaaaatta ggtcttcaac
     7501 atgtacatgc cctgatgata attgatttaa atggatgatg gcattagtga tatcattatc
     7561 tgttccatcg accacaatac cttgtttttt cttgattgtt aaagcgatat taaatttttg
     7621 acaccattgt tgaattcgaa cgaaatagtc gttgagcaca gttttagata atagataatc
     7681 ttgctctagt tgttgcaatg tctgcgattt attgtgcatt agcagttgat acgccataga
     7741 taataatagt ttttctttat atgattgaat atgcgtgtaa cgttcagtta tcgtttctaa
     7801 ggtatactga gatgtattta attgatatcc tttagattta atacttacga tgatatcatc
     7861 cataaaattg ctattgataa catggatatc attgcgaact gtgcggtttg aaacattgac
     7921 atgtttagca atttcattag aactaatgtg ctttgatgga tttttaataa aaaactggag
     7981 tagttttaag tgtctatcaa gcatttaaca tgtacctcct ttctaaattt ttcgtgtaag
     8041 cgtttttaag ggtgtattaa tattattaaa catgagagct tatacatacg tcaatgacat
     8101 taaagcgaac ttttatatga ttttaacaga gtgcgaatta tgcaaataaa gaacagcagt
     8161 aagatatttc aaatagaaaa atatctcact gctgtttttc tgaatttatg catctggtac
     8221 ttgtggacgt atcaggcaaa tgattaattt ttaggtgatt gtgcttgagg tgtttgttta
     8281 gaaggcgtat tggtattatt tgatttatta gcaggtggtg tgttctgttg ttgattattt
     8341 tggttgtttg ttgacgattg actgccacca ccgtgagtat tatttttatt tgaatttgag
     8401 tcttttggct gagctggttg cgtattcgat ggtactgttg aatggttgtt aggaacattt
     8461 gacggtgaag atggttgatt gtcattagta acgttattgc cattattttg atttttatct
     8521 gttttgtctg cactatcatc ttgttgatca ttttcttcgg tttctgtctt tttatgcgta
     8581 gatttatttt cttgttcttg gttattactt gatgatgtag aatgatttat tgcagcataa
     8641 attgcgaacc caagtgctcc gattaatacg ataattacga tggtaataag aagttttttc
     8701 aaatataacg cctccatgtt aatgaaaagt agttgatacg tgaagctaaa taatttattt
     8761 caatattgcc ataatcattt taaaaaataa atattagaaa agtaggcgat attaatattt
     8821 tcattgtttt ttgtgtactt caattagtat aacaaaactg ttatgtattt ataaatataa
     8881 tcattagtat ttttatggct gaaaaagtta taataaaagt gtaaggaata aaatatttgt
     8941 atggaaaaga gagataatta tgaataaaac cagtaaagtt tgtgtagcag caacattagc
     9001 attgggcaca ctgattggag taacagttgt tgaaaattcg gcgccaacaa gtaaacaggc
     9061 acaggcagca ataaccccat attatactta taatggttat attggtaata atgctaattt
     9121 tattctggat aagaatttta ttaacgcgat taagtatgat aatgtgaaat tcaatggtat
     9181 taaattagct aaaacgaata cgataaaaaa agtagagaaa tatgatcaaa cttttaaagg
     9241 tgtttctgca aaaggaaacg aagcaagtca attgcaattt gtagttaaaa ataatatttc
     9301 attaaaagat atccaaaaag cttatggcaa ggacttgaaa aaagaaaatg gtaaaacaaa
     9361 ggaagctgat agcggtattt tttactatca aaatgctaaa aagacattag gcatctggtt
     9421 tgtcgttgat cataatagag ttgtcgaagt aacagttgga catacaccat acaaaacaag
     9481 taaataaaat aatggcatat taaggctaga gtgtgaggag tgataccgca ttctagtctt
     9541 ttttattaaa taataacgat tattgcgtct tacatagttg tttgaaattg ataaaaatgt
     9601 ttatttgatt atgattattt aaatagttat ct
//
LOCUS       DAKTLH010000096         9514 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.096, whole genome shotgun sequence.
ACCESSION   DAKTLH010000096 DAKTLH010000000
VERSION     DAKTLH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9514
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.096"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            204..1100
                     /gene="xerC"
                     /locus_tag="O0394_001906"
     CDS             204..1100
                     /gene="xerC"
                     /locus_tag="O0394_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCY0499978.1"
                     /translation="MNHIQDAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLEL
                     NTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKNLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            1097..1642
                     /gene="hslV"
                     /locus_tag="O0394_001907"
     CDS             1097..1642
                     /gene="hslV"
                     /locus_tag="O0394_001907"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCY0499979.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            1708..3111
                     /gene="hslU"
                     /locus_tag="O0394_001908"
     CDS             1708..3111
                     /gene="hslU"
                     /locus_tag="O0394_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCY0499980.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            3136..3909
                     /gene="codY"
                     /locus_tag="O0394_001909"
     CDS             3136..3909
                     /gene="codY"
                     /locus_tag="O0394_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCY0499981.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            3960..4052
                     /locus_tag="O0394_001910"
     CDS             3960..4052
                     /locus_tag="O0394_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499982.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDISH"
     gene            4251..5018
                     /gene="rpsB"
                     /locus_tag="O0394_001911"
     CDS             4251..5018
                     /gene="rpsB"
                     /locus_tag="O0394_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCY0499983.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            5052..5165
                     /locus_tag="O0394_001912"
     CDS             5052..5165
                     /locus_tag="O0394_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499984.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            5200..6081
                     /locus_tag="O0394_001913"
     CDS             5200..6081
                     /locus_tag="O0394_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCY0499985.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            6218..6940
                     /locus_tag="O0394_001914"
     CDS             6218..6940
                     /locus_tag="O0394_001914"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCY0499986.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            6959..7513
                     /gene="frr"
                     /locus_tag="O0394_001915"
     CDS             6959..7513
                     /gene="frr"
                     /locus_tag="O0394_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCY0499987.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
     gene            7886..8656
                     /locus_tag="O0394_001916"
     CDS             7886..8656
                     /locus_tag="O0394_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCY0499988.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            8663..9445
                     /locus_tag="O0394_001917"
     CDS             8663..9445
                     /locus_tag="O0394_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCY0499989.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
BASE COUNT         3539 a         1302 c         1864 g         2809 t
ORIGIN      
        1 tatttaaaag gcaatgaaat aataaatggc aaggctatta ataaagactt ttagtaatta
       61 atttaaaaaa gaggtattct aattaacagg ttttccgatt agttacaatt atttaattct
      121 caaaagattt agaattgatt atcaaattac tgtaagccct ttgctgtata tgctacaatt
      181 cttattgatg gagggtaaat gtattgaatc atattcaaga tgcgttttta aatacattga
      241 aagttgaacg gaatttttcg gaacacacat tgaaatcata tcaagatgac ttaattcagt
      301 ttaatcaatt tttagaacaa gaacatttag agttgaatac ttttgaatac agagatgcta
      361 gaaattattt gagctattta tattcaaatc atttgaaaag aacatctgtt tctcgtaaaa
      421 tctcaacgtt aagaactttc tatgaatatt ggatgacgct tgatgagaac attattaatc
      481 catttgttca attagtacat ccgaaaaaag aaaaatatct tccgcaattc ttttacgaag
      541 aagaaatgga agcgttattc aaaactgtag aagaggacac ttcaaaaaat ttacgggatc
      601 gagttattct tgaattgttg tatgctacag gcatccgtgt ttcggaatta gtaaatatta
      661 aaaaacaaga tatagatttt tacgcgaatg gtgttaccgt attaggaaaa gggagcaaag
      721 agcgctttgt accgtttggt gcttattgta gacaaagcat cgaaaattat ttagaacatt
      781 tcaaaccaat tcagtcatgc aatcatgatt ttcttattgt aaatatgaag ggtgaagcaa
      841 tcactgaacg cggtgtacga tatgttttaa atgatattgt taaacgaaca gcaggcgtaa
      901 gtgagattca tccccacaag ctcagacata catttgcaac gcatttattg aatcaaggtg
      961 cagacctaag aacagtacaa tcgttattag gtcatgttaa tttgtcaaca actggtaaat
     1021 atacacacgt atctaaccaa caattaagaa aagtgtatct aaatgcacat cctcgagcga
     1081 aaaaggagaa tgaaacatga gtaatacaac attacatgca acaacaattt atgctgtaag
     1141 acataatggg aaagcagcta tggctggaga tgggcaagta acgcttggtc aacaagtcat
     1201 catgaaacaa acggcaagaa aagtgcgacg tttatatgaa ggtaaagtgt tagctggttt
     1261 cgcaggtagt gtagcagatg cgtttacgtt atttgaaaaa ttcgaaacaa aattacaaca
     1321 gtttagtggt aacttagaaa gagctgctgt tgaattggca caagaatggc gaggcgataa
     1381 acaattacgt caattagaag ctatgctaat tgtaatggat aaagatgcta ttttagttgt
     1441 cagtggaact ggcgaagtta ttgctccaga tgatgacctt atcgctattg gatcaggagg
     1501 caactacgca ttaagcgcag gacgtgcatt gaaacgccat gcatcgcatt tgtctgctga
     1561 agaaatggca tatgagagct tgaaagtagc ggctgatatt tgtgtcttta ccaacgataa
     1621 tattgttgtc gaaacactat aataatcaga gcacgataaa taattacgag caattaattt
     1681 tagttaaaag acggaggaat gaaattaatg gatacagctg gaataagatt aactccaaaa
     1741 gaaatcgtat ctaaattaaa tgaatacatc gttggacaaa atgatgctaa acgtaaagtg
     1801 gcaattgccc tacgtaatcg atacagaaga agtttattag atgaggaatc aaagcaagaa
     1861 atttcaccta aaaatatttt gatgattgga ccaaccggcg ttggtaaaac tgaaattgca
     1921 agaagaatgg ccaaagttgt cggcgcgcca tttataaaag tagaagctac taaatttact
     1981 gaggtaggtt atgtaggacg agatgttgaa agtatggtta gagatcttgt tgatgtttca
     2041 gtaagattag tcaaggcgca gaaaaaatca ttggtacaag atgaagcaac agctaaggcc
     2101 aatgaaaaac ttgttaagtt attagttcca agtatgaaaa agaaagcgtc tcaaacgaat
     2161 aatcctttag agtcactttt cggaggtgca attccaaatt tcggacaaaa taacgaagat
     2221 gaagaagaac cacctactga ggaaattaaa acaaaacgtt ctgaaattaa gagacagcta
     2281 gaagaaggca aacttgaaaa agaaaaggta agaattaaag tcgaacaaga tcctggtgct
     2341 ttaggtatgc taggtacaaa tcaaaatcag caaatgcaag agatgatgaa tcaattaatg
     2401 cctaaaaaga aagttgagcg agaagttgct gttgagacgg caaggaaaat cttagctgat
     2461 agttatgcgg atgaactaat tgatcaagaa agcgctaacc aagaagcgct tgaattagca
     2521 gaacaaatgg gtatcatctt tatagatgaa atcgacaaag ttgcgacgaa taatcataat
     2581 agtggtcaag atgtctcaag acaaggtgtt caaagagata ttttacctat acttgaaggt
     2641 agcgttattc aaaccaaata tggtactgtg aatactgaac atatgctgtt tataggtgct
     2701 ggagctttcc atgtatctaa gccgagtgac ttgataccag aattgcaagg tcgttttccg
     2761 attagagttg aacttgatag tttatcggta gaagattttg taagaatttt gacagaacca
     2821 aaattgtcat taattaaaca atatgaagca ttgcttcaaa cagaagaagt tactgtaaac
     2881 tttaccgatg aagcaattac tcgcttagct gagattgctt atcaagtaaa tcaagataca
     2941 gacaacattg gtgcacgtcg acttcataca attttagaaa agatgctaga agatttatca
     3001 ttcgaagcac caagtatgcc gaatgcagtt gtagatatta ccccacaata tgttgatgat
     3061 aaattaaaat caatttcaac aaataaagat ttaagtgcat ttattctata aaaatataca
     3121 aaaggagaaa aattcatgag cttattatct aaaacgagag agttaaacac gttacttcaa
     3181 aaacacaaag gtattgcggt tgattttaaa gatgtagcac aaacgattag tagcgtaact
     3241 gtaacaaatg tatttattgt atcgcgtcga ggtaaaattt taggatcgag tctaaatgaa
     3301 ttattaaaaa gtcaaagaat tattcaaatg ttggaagaaa gacatattcc aagtgaatat
     3361 acagaacgat taatggaagt taaacaaaca gaatcaaata ttgatatcga caatgtatta
     3421 acagtattcc cacctgaaaa cagagaatta ttcatagata gtcgtacaac tatcttccca
     3481 attttaggtg gaggggaaag attaggtaca ttagtacttg gtcgagtaca tgatgatttt
     3541 aatgaaaatg atttggtact aggtgaatat gctgctacag ttattggtat ggaaatctta
     3601 cgtgagaagc atagtgaagt agaaaaagaa gcgcgcgata aagctgctat tacaatggca
     3661 attaattcat tatcttattc tgaaaaagaa gcgattgaac atatctttga agaacttggc
     3721 ggtacggaag gcctattaat cgcatcaaaa gttgcagata gagttggtat tactagatct
     3781 gtaattgtaa atgcactacg taaattagaa agtgctggtg taattgaatc acgttcttta
     3841 ggaatgaaag gtactttcat taaagttaaa aaagaaaaat tcttagatga attagaaaaa
     3901 agtaaataag tcgatgagtc tgggacataa ttcaatgtcc tagtcgtgac gatgttatct
     3961 tggcggtagt caatgaaatg actttgatga ttatgttgaa gtcatttctt tatttttgtc
     4021 taattttcaa tcaaattgat atttcacatt aacttgatag aattaccttt aaatttatgg
     4081 aaaaatgctt gaaaaacatt tgctttagac acggcattat gttatattta ttttcggcta
     4141 taaagccaat acacacatat caaacgtgat tgtgtaaagg gtgcaatatt tatatattgt
     4201 ctttacaata gtttgctatg gaggtaatta accaatagga ggaatttata atggcagtaa
     4261 tttcaatgaa acaattacta gaagcgggtg ttcacttcgg tcaccaaaca cgtcgttgga
     4321 acccaaaaat gaaaaaatat atcttcactg agagaaatgg tatttatatc atcgacttac
     4381 aaaaaacagt gaaaaaagta gacgaggcat acaacttctt gaaacaagtt tcagaagatg
     4441 gtggacaagt cttattcgta ggaactaaaa aacaagcaca agaatcagtt aaatctgaag
     4501 cagaacgtgc tggtcaattc tacattaacc aaagatggtt aggtggatta ttaactaact
     4561 ataaaacgat ctcaaaacga atcaaacgta tttctgaaat tgaaaaaatg gaagaagatg
     4621 gtttattcga agtattacct aaaaaagaag tagtagaact taaaaaagaa tacgaccgtt
     4681 taatcaaatt cttaggcgga attcgtgata tgaaatcaat gcctcaagca ttattcgtag
     4741 ttgacccacg taaagagcgt aatgcaattg ctgaagctcg taaattaaat attcctatcg
     4801 taggtatcgt tgacactaac tgtgatcctg acgaaattga ctacgttatc ccagcaaacg
     4861 acgatgctat ccgtgcggtt aaattattaa ctgctaaaat ggcagatgca atcttagaag
     4921 gtcaacaagg cgtttctaat gaagaagtag ctgcagaaca aaacatcgat ttagatgaaa
     4981 aagaaaaatc agaagaaaca gaagcaactg aagaataatc aactgttgaa tctgacttag
     5041 atatagttta aatgggtgat aagatattaa tgcttatcac cttttttaaa aagaaaatcg
     5101 aggcaaatta caaatattca attagagtat tggcaatctt gcctataata atgctaaaat
     5161 cataatatat aaaatgataa cttattggag gaataatgaa tggcaactat ttcagcaaaa
     5221 cttgttaaag aattacgtga aaaaactggc gcgggtatga tggattgtaa aaaagcgcta
     5281 actgaaactg atggtgacat cgataaagcg attgactacc tacgtgaaaa aggtattgct
     5341 aaagcagcta aaaaagcaga ccgtattgcg gctgaaggtt tagtacatgt agaaactaaa
     5401 ggtaacgacg cagttatcgt tgaaatcaac tctgaaacag actttgttgc tcgtaacgaa
     5461 ggtttccaag agttagttaa agaaatcgct aatcaagtat tagatacaaa agctgaaact
     5521 gttgaagctt taatggaaac aactttacca aatggtaaat cagttgatga aagaattaaa
     5581 gaagcaattt caacaatcgg tgaaaaatta agtgttcgtc gttttgctat cagaactaaa
     5641 actgataacg atgctttcgg cgcttactta cacatgggtg gacgcattgg tgtattaaca
     5701 gttgttgaag gttcaactga cgaagaagca gcaagagacg ttgctatgca tatcgctgca
     5761 atcaacccta aatatgtttc ttctgaacaa gttagcgaag aagaaatcaa ccacgaaaga
     5821 gaagttttaa aacaacaagc attaaatgaa ggtaaaccag aaaacatcgt tgaaaaaatg
     5881 gtggaaggac gtttacgtaa atacttacaa gaaatttgtg ctgtagatca agacttcgtt
     5941 aaaaaccctg atgtaacagt tgaagctttc ttaaaaacaa aaggtggaaa acttgttgac
     6001 ttcgtacgct atgaagtagg cgaaggtatg gaaaaacgcg aagaaaactt tgcggatgaa
     6061 gttaaaggac aaatgaaata atctgtcata aagtaaaaca aggaagaaga cacctttaat
     6121 gttgctttat taaaatgtaa atcattctaa taaaacgaca actgtgtctt ctttacttgt
     6181 atatgttaca tatattcacg atagagagga taagaaaatg gctcaaattt ctaaatataa
     6241 acgtgtagtt ttgaaactaa gtggtgaagc gttagctgga gaaaaaggat ttggcataaa
     6301 tccagtaatt attaaaagtg ttgctgagca agtggctgaa gttgctaaaa tggactgtga
     6361 aatcgcagta atcgttggtg gcggaaacat ttggagaggt aaaacaggta gtgacttagg
     6421 tatggaccgt ggaactgctg attacatggg tatgcttgca actgtaatga atgccttagc
     6481 attacaagat agtttagaac aattggattg tgatacacga gtattaacat ctattgaaat
     6541 gaagcaagtg gctgaacctt atattcgtcg tcgtgcaatt agacacttag aaaagaaacg
     6601 cgtagttatt tttgctgcag gtattggaaa cccatacttc tctacagata ctacagcggc
     6661 attacgtgct gcagaagttg aagcagatgt tattttaatg ggcaaaaata atgtagatgg
     6721 tgtatattct gcagatccta aagtaaacaa agatgcggta aaatatgaac atttaacgca
     6781 tattcaaatg cttcaagaag gtttacaagt aatggattca acagcatcct cattctgtat
     6841 ggataataac attccgttaa ctgttttctc tattatggaa gaaggaaata ttaaacgtgc
     6901 tgttatgggt gaaaagatag gtacgttaat tacaaaataa atttagaggt gtaaaataat
     6961 gagtgacatt attaatgaaa ctaaatcaag aatgcaaaaa tcaatcgaaa gcttatcacg
     7021 tgaattagct aacatcagtg caggaagagc taattcaaat ttattaaacg gcgtaacagt
     7081 tgattactat ggtgcaccaa cacctgtaca acaattagca agcatcaatg ttccagaagc
     7141 acgtttactt gttatttctc catacgacaa aacttctgta gctgacatcg aaaaagcgat
     7201 aatagcagct aacttaggtg ttaacccaac aagtgatggt gaagtgatac gtattgctgt
     7261 acctgcctta acagaagaac gtagaaaaga gcgcgttaaa gatgttaaga aaattggtga
     7321 agaagctaaa gtatctgttc gaaatattcg tcgtgatatg aatgatcagt tgaaaaaaga
     7381 tgaaaaaaat ggcgacatta ctgaagatga gttgagaagt ggtactgaag atgttcagaa
     7441 agcaacagac aattcaataa aagaaattga tcaaatgatt gctgataaag aaaaagatat
     7501 tatgtcagta taaaactaat atacaatgac atattaaaat gccagtatta aacgataatg
     7561 taacatttaa aatgggcatg tttaattaaa tcaaagatgc atgtgataat ttaaattcac
     7621 aatgagcata aaaatggtgt ttaaacaagt taattaaaca tatactttat aaataatagg
     7681 cattaggtat attgctataa taaagttatg taatttttaa cctcagtatg tatgtcacat
     7741 ttctggtgta aactgtaccg agtcagactt tggtacagtt tttttatttg cttattcaat
     7801 gcattaaatg agtatgataa aatgataatg attgtttagt aacttatact atatgacaga
     7861 gatgatcagg ctcggaggaa agaccatgtt taaaaagcta ataaataaaa agaacactat
     7921 aaataattat aatgaagaat tagactcgtc taatatacct gaacatatcg ctattattat
     7981 ggatggtaat gggcgatggg ctaagaagcg aaaaatgcct agaattaaag gtcattacga
     8041 aggtatgcaa acaataaaaa aaattactag ggtagctagt gatattggtg ttaagtactt
     8101 aactttatac gccttttcca ctgaaaattg gtcaagacct gaaagtgaag taaattatat
     8161 tatgaatttg cctgtcaatt tcttaaagac attcttaccg gaactaattg aaaaaaatgt
     8221 caaagttgaa acaattggat ttactgataa gttgccaaaa tcaacgatag aagcaattaa
     8281 taatgctaaa gaaaagacag ctaataatac cggcttaaaa ttaatatttg caattaatta
     8341 tggtggcaga gcagaacttg ttcatagtat taaaaatatg tttgacgagc ttcatcaaca
     8401 aggtttaaat agtgatatca tagatgaaac atatataaac aatcatttaa tgacaaaaga
     8461 ctatcctgat ccagagttgt taattcgtac ttcaggagaa caaagaataa gtaatttctt
     8521 gatttggcaa gtttcgtata gtgaatttat ctttaatcaa aaattatggc ctgactttga
     8581 cgaagatgaa ttaattaaat gtataaaaat ttatcagtca cgtcaaagac gctttggcgg
     8641 attgagtgag gagtagtata gtatgaaagt tagaacgctg acagctatta ttgccttaat
     8701 cgtattcttg cctatcttgt taaaaggcgg ccttgtgtta atgatatttg ctaatatatt
     8761 agcattgatt gcattaaaag aattgttgaa tatgaatatg attaaatttg tttcagttcc
     8821 tggtttaatt agtgcagttg gtcttatcat cattatgttg ccacaacatg cagggccatg
     8881 ggtacaagta attcaattaa aaagtttaat tgcaatgagc tttattgtat taagttatac
     8941 tgtcttatct aaaaacagat ttagttttat ggatgctgca ttttgcttaa tgtctgtggc
     9001 ttatgtaggc attggtttta tgttctttta tgaaacgaga tcagaaggat tacattacat
     9061 attatatgcc tttttaattg tttggcttac agatacaggg gcttacttgt ttggtaaaat
     9121 gatgggtaaa cataagcttt ggccagtaat aagtccgaat aaaacaatcg aaggattcat
     9181 aggtggcttg ttctgtagtt tgatagtacc acttgcaatg ttatattttg tagatttcaa
     9241 tatgaatgta tggatattac ttggagtgac attgatttta agtttatttg gtcaattagg
     9301 tgatttagtg gaatcaggat ttaagcgtca tttcggcgtt aaagactcag gtcgaatact
     9361 acctggacac ggtggtattt tagaccgatt tgacagcttt atgtttgtgt taccattatt
     9421 aaatatttta ttaatacaat cttaatgctg agaacaaatc aataaacgta aagaggagtt
     9481 gctgagataa tttaatgaat ctcagaactc cttt
//
LOCUS       DAKTLH010000097         9458 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.097, whole genome shotgun sequence.
ACCESSION   DAKTLH010000097 DAKTLH010000000
VERSION     DAKTLH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9458
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.097"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            111..755
                     /locus_tag="O0394_001918"
     CDS             111..755
                     /locus_tag="O0394_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCY0499990.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN"
     gene            871..1737
                     /locus_tag="O0394_001919"
     CDS             871..1737
                     /locus_tag="O0394_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0499991.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKEWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
     gene            1938..3158
                     /locus_tag="O0394_001920"
     CDS             1938..3158
                     /locus_tag="O0394_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HCY0499992.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDVIST"
     gene            3155..3643
                     /locus_tag="O0394_001921"
     CDS             3155..3643
                     /locus_tag="O0394_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HCY0499993.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(3742..4431)
                     /locus_tag="O0394_001922"
     CDS             complement(3742..4431)
                     /locus_tag="O0394_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HCY0499994.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKNGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIER
                     NISKKHSHTFL"
     gene            4558..5001
                     /locus_tag="O0394_001923"
     CDS             4558..5001
                     /locus_tag="O0394_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0499995.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQVESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISNTIHKNNLPMISLNKDLGYQVSHVKMYKDID"
     gene            5068..5463
                     /locus_tag="O0394_001924"
     CDS             5068..5463
                     /locus_tag="O0394_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499996.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKDKEASQDKQSK
                     DQTKSSDKDNHKKPTSADKDQKANDKHQS"
     gene            5601..5900
                     /locus_tag="O0394_001925"
     CDS             5601..5900
                     /locus_tag="O0394_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0499997.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGHTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            5980..6522
                     /locus_tag="O0394_001926"
     CDS             5980..6522
                     /locus_tag="O0394_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0499998.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEEHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(6618..7184)
                     /locus_tag="O0394_001927"
     CDS             complement(6618..7184)
                     /locus_tag="O0394_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCY0499999.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(7186..7644)
                     /locus_tag="O0394_001928"
     CDS             complement(7186..7644)
                     /locus_tag="O0394_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCY0500000.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            complement(7647..8330)
                     /locus_tag="O0394_001929"
     CDS             complement(7647..8330)
                     /locus_tag="O0394_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500001.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            complement(8502..9377)
                     /locus_tag="O0394_001930"
     CDS             complement(8502..9377)
                     /locus_tag="O0394_001930"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCY0500002.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNDQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QISYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
BASE COUNT         3308 a         1359 c         1588 g         3203 t
ORIGIN      
        1 agtaaaataa taataaatat tttcatatga ttaacaaaaa ctataaaact gtatcatgac
       61 atgacatcat ttatgtagtt ttatataata atgacaagga gttggtgact gtgaaagtaa
      121 agtatataga taaacgtcac tggcgtcgcc taattgatag ggaatacaca gaggtaaaag
      181 ttaataataa taggtttaag ggtattatag gcttagtcac gatgaaaaag gttcgtgatc
      241 ctttagaggt gacggtagtt ggacaaaata tcattgtcgc agatgacaat tataaatggt
      301 tgcaaatact acctgaaaag aaacgttata gtataactgt aatgtttgat aataaaggca
      361 atccattaga atattatttt gatataaata tcaaaaatat aacgcaaaaa ggtaatgcgc
      421 gtacagtaga tttatgttta gatgttttag cattaccaag tggtgaatat gagttggtag
      481 atgaagatga tttaatgttt gcattagaaa gtgagcaaat tacaaaaaag caatttcatg
      541 aagcatatat gattgcacat caaattatgg cagagttaga aaatgatttt aaaggattcc
      601 aaaagaaaat catgtactgc tttaataaaa ttaatgcaaa ggctcaaaaa aatcatcaaa
      661 agccacaaaa taaaactaat attgaaaaaa gcaaacaaat aaagcctaag caatataatc
      721 aaactaaaaa tcaccaacaa caaaagaaaa actaagtaat tcaagctgca gccataccaa
      781 taaaattggt gaaattattc gttagtaatg aactcatgaa tgcttgtagc catgaaagtt
      841 caataattga ataatttatg gggggaaatt atgaagattg aagactatcg tttactaata
      901 acattagacg aaacgaaaac gttacgtaaa gcggctgaaa ttttatatat atctcaacct
      961 gctgttacac aaagactaaa agctattgaa aatgcttttg gagtagatat ttttatcaga
     1021 acaaaaaaac aattgattac aacaactgaa ggaacaatga ttattgagca tgctcgtgac
     1081 atgttgaaaa gagagcgatt attttttgac aaaatgcagg cacatattgg tgaagtgaat
     1141 ggaacaatat caatcgggtg ttcttctttg attggacaaa ccttacttcc tgaagttttg
     1201 agcctatata atgcccaatt tcctaatgtt gaaatacaag tgcaagttgg ttcaactgaa
     1261 caaattaaag caaatcatag agattatcat gttatgataa ctcgtggaaa taaggtaatg
     1321 aatttagcta acacacattt atttaatgat gatcattatt ttatttttcc aaaaaataga
     1381 cgagatgatg ttacaaagtt accatttata gagtttcaag ctgatccgat ttatataaat
     1441 caaataaaag aatggtataa cgataattta gaacaagatt accatgcaac tattacagtg
     1501 gatcaagtag caacttgcaa agaaatgttg attagtggtg taggtgttac aatcttgcca
     1561 gaaattatga tgaaaaatat cagcaaagaa caatttgagt ttgaaaaagt agaaattgat
     1621 aatgaaccgc tgattcgttc gacatttatg agttatgatc cgagcatgtt gcaattgcca
     1681 caagttgatt cttttgtaaa tctcatggcg agctttgttg aacaaccaaa ggcgtagttt
     1741 tagactaatt taaggtttgt atttaatttt aaactattcg gttaaattga acgtagttgg
     1801 ttgctaatgc accaacagca aaagagcccc taattaataa attaaaaggg gacaaaggaa
     1861 tacagttgga tgctaacgca ccaactgcat aagagcccct aattaataaa ttaaaagggg
     1921 ctctaagaag cggggttatg gatatgtttg cagcgttatt acaaataaag aattataaac
     1981 tctttgttgc taatatgttt ctactaggta tgggtattgc ggttacggtc ccatatcttg
     2041 ttctttttgc aactaaagat ttaggtatga caacaaatca gtatggatta cttctagcat
     2101 ctgcagcgat tagccagttt acagtaaatt caattattgc tagattttcg gatacgcatc
     2161 actttaatag aaaaattatt attattctcg cattattaat gggtgcgctt ggtttttcaa
     2221 tatacttttt tgtagataca atctggttat tcatattact atatgcgatt ttccaaggat
     2281 tatttgcacc agcaatgccc caactttacg catctgctag agaatctatc aatgtttcaa
     2341 gctctaaaga tagagctcaa tttgccaaca cagtattacg ttcaatgttc tcattgggct
     2401 ttttatttgg tccatttatt ggtgcccaat taatcggatt aaaaggctat gctggattgt
     2461 ttggtggaac aataagtatc attttattta ctttagtact tcaagtgttt ttctataagg
     2521 atttaaacat taaacaccct attagtacgc aacaacatgt tgaaaaaatt gctcctaata
     2581 tgtttaaaga caaaacgctt ttattaccat ttattgcatt tattttatta cacattggac
     2641 aatggatgta tacgatgaat atgcctttat ttgttactga ttatttaaaa gaaaatgaac
     2701 aacatgtcgg ttatttagct agtttatgtg ctggtttaga agtgccattt atgatcattc
     2761 ttggcgtttt atcatctaga ttacagactc gaacattgtt gatttatgga gcgatttttg
     2821 gtggtttatt ctacttcagc attggggtat ttaaaaactt ctatatgatg ttagcaggac
     2881 aggtgttttt agctattttc ttagcggttc ttttaggaat tggtattagt tatttccaag
     2941 atatcttacc agattttcca ggatacgctt caacactatt ttctaatgca atggttattg
     3001 gacagttagg cggtaaccta ttaggtggtg ctatgagtca ctgggtaggt ttggaaaatg
     3061 tattttttgt atcagcagca tcaatcatgt taggtatgat acttatattc tttactaaaa
     3121 atcaaaaaat tacaaaagag gatgtgatat caacatgaca attattttat ggctacttat
     3181 catcgctgcc ttcatgttag catttgttgg gttgattaag ccgattattc cttctgtttt
     3241 agtattatgg gttggctttt taatctatca atttggcttt cataatcagc atttatcatg
     3301 ggtgttttat gtatctatgg cattgctaac aatattaatt ttatgtgccg actttttagc
     3361 taataaatat tttgtgaatc gcttcggtgg ttctaagttt ggagagtatg cagctttaat
     3421 tggtgtggtt attggatgtt ttgttttacc gccatttgga attattatta taccttttat
     3481 tttggtattc atagttgaat taatacaagg ctattcattt gaaagagcag ttaaagtaag
     3541 tataggttca atcgtagcat ttttaacaag tagtatagct caagcaatca ttatgtttat
     3601 aatgattgta tggttcttta tagatgcttt attgattaat taataaaaag cttattgcaa
     3661 aatatgtttt tcggtaactg taatttagtg attttatcat taacagtacc aaattcgcat
     3721 atgatgtaat aagctttttg tttataaaaa tgtatgagaa tgttttttcg aaatatttct
     3781 ttcaatgcgt aatccaatag gcataactat aaatgaaaat ataataaata cccaattgct
     3841 tacctgaata tatggtccaa aggcaagtaa tgattgtgga ataaagaata catagaaaaa
     3901 tcctacaatt agtagaatga ttgctaaata ggtgaatatc catatccaat ttgaattgtt
     3961 aaaacactgc agttgtattg ttttaaaact aaaccatata aataaaaaga caataaagaa
     4021 tccaacgact actgaaaacg ggaatgaaac aaaatataaa ttacttccat ttttttccat
     4081 gaaaaatcct aaaaatcctt tgagaaaact aacaatccca attaatagaa cgatgtgttg
     4141 atagatatat ttaaaaatat ttttaaatgt ttcattaggc atcgctttta gttcttttat
     4201 tgcatgtgct tttgggtcgt gattgaaaaa atctaaggct aataaaccat gttgttctgc
     4261 gcttaataat tgtttgagta tacggttaat aattaactct gtatcatgag ggttgacgcg
     4321 aaagtcagag cgcatataag tcatataatt ctcgaagatt tctctatcag tattgcttaa
     4381 tcttaatgat ttaacattat tttcttttgt taattgcgca gtacttttca ttgttactta
     4441 agcgctcctt taaaaatgtt taattccaaa ttaaaatgga aatgatttta tagtattaat
     4501 aaggtcaatc atatcatatt aaacgcataa atataacgat taatattgga gaggaaaatg
     4561 aggacactta ataaagatga acataattat atcaagcaaa tagctaatat acatgagaca
     4621 ttattgtcgc aagtagaatc caactataaa tgtactaaac tgagtattgc tcttaggtac
     4681 gagatgatat gttcaagatt agaacataca aatgataaaa tttatatata tgaaaatgaa
     4741 ggtcaattaa tagcgtttat ttggggacat tttagtaatg aaaaaagtat ggttaacatt
     4801 gaactgctat atgttgaacc acaatttcgc aaactgggaa tagctacgca actgaagatt
     4861 gcgcttgaaa aatgggcaaa aactatgaat gcaaagcgaa taagcaatac aattcataaa
     4921 aataatttgc caatgatatc tttgaataaa gatttaggtt atcaagtgag tcatgtgaaa
     4981 atgtataaag atattgatta gaattaggat tatgttgcta attcatgtta aaattaaaaa
     5041 agatttaatg acgttaagga gttttatatg aagaaattaa tcatcagtat tatggcggtc
     5101 atgctatttt taacaggttg tggtaaaagt caagagaaag ccactctgga aaaggatatc
     5161 gataatttac aaaaagaaaa taaagaatta aaagacaaaa aagaaaagct tcaacaagaa
     5221 aaagaaaaat tagcagataa gcaaaaagac cttgaaaaag aagtgaaaga tttaaaacct
     5281 tcaaaagaag ataacaagga tgataaaaaa gacgaagaca aaaataaaga caaagataaa
     5341 gataaagagg catcacaaga taagcaatca aaagatcaaa ctaagtcatc ggataaagat
     5401 aatcacaaaa agcctacatc agcagataaa gatcaaaaag ctaatgacaa acaccaatca
     5461 taatcgaatt gcttacttgt tatagatgaa aggtacagcg ttttaaacct tattttaagg
     5521 gtatgtatta attaaaatgt ggtcatgatt gaaacagaac gtaaaaatag acaacgtaat
     5581 taataaagga gagaaacggc atgcatgaac aagattttag aattttagag ggtcaagata
     5641 ttactttgcc agaattaggt agagaattag aaaatattac aggacatacg attgctgatt
     5701 ctactggcga aattaagcgt gtaattgcac atttaccaaa ctttgagtcc gatacagata
     5761 cttttgttgc tacatatcgt ttaaaccatc aacaagattt tatagatgca acttttactg
     5821 cgctgaaatc agatagagca cgtttaaaag aagtgccagt tcatgttgaa cttataagtt
     5881 atatttctaa atcaaaataa actgctatct aaaacgcaaa gttgatcaaa atatcgattt
     5941 tgtgtttttt attgagaaat tatataggag tgtcaatcga tgatttattg tgaaacagag
     6001 cgtttaatat taagagactg gcatgaagat gatctgttac cttttcaaaa aatgaatgcg
     6061 aattatgacg tacgtaaata ttttccaagt ttattgagtt atcgtcgttc agaattagat
     6121 atgagaacta tggatgcggt tattaaagat tatggcattg gattatttgc tgtagaagat
     6181 aaagagtccc atcaatggat aggctttata ggtttgaatt atattccaga aacaagcgat
     6241 tatccattta aagaattacc gctttatgaa ataggttggc gcttgttgcc agaattttgg
     6301 ggaaaaggat tagcaactga aggcgcaaag gcaacattga agttagcaga agaacatcaa
     6361 atatacgatg tctatagttt tacagcagaa gcaaataaag cttcacaacg tgtaatggaa
     6421 aaaattggca tgacagtgta tgatcatttc gaattaccca atctaagtaa gtatcattta
     6481 ttaaaaaggc aagtgcgcta ttacattaat cttcgaaagt gaaaaattta tacataagcg
     6541 taacaaacac ccctaacatt gtttagctga tgataacaat attaggggtg tttgtttatt
     6601 ttttaacctt agaatgatta atcgtatgaa cgagtaccca gaggtttgaa atttaatatt
     6661 gattcaatta atgattcctt agtgtcgcat aacggtgcaa gagcacgata cttaggatca
     6721 ataaaacctt cctcaatcat atggtcaatc attgtttgta gtggattgaa aaagccatta
     6781 atattataaa tggcaatagg cttttcatgg atacctattt gagcccaact atacatttcg
     6841 aaaaattctt ctagtgaacc tgcgccacca ggagccatga caaatgcatc tgcaagttct
     6901 gccattttat ttttacgttc atgcatagaa tcaactaaaa ttaattcagt taaacgttgg
     6961 cttgtgattt catgttcatc taacatttta ggcatgacgc caatagcttt gccgccatga
     7021 tctaatacac catcttgaat ggcacccata atgccaattg accctgcacc aaatactaat
     7081 tcataacctt gttcagcaaa atatttacct aaatcgtatg ctttttgtac atatgaaggg
     7141 tcatgacctt tgcttgcacc acaataaact gcgattcgtt tcatgttaat ccagctcctt
     7201 aattcgatga atgactttta atagtgattg ttcaaacact ttttgatctt gctttgtaaa
     7261 aggtggggga cctttgtggc gaccaccttg ttttctaatt tgtgcattca tatatcgttt
     7321 atctaatagt tgttgaatat ttttggaatt gtatatcttc ccattatgat gcatgacaat
     7381 taagactttg tcgactaata aacttgcgag tccataatct tgagtgacta cgatatcatc
     7441 cttcgttgat aattgaacaa ttttgtaatc aactgcatct ggtccatcat caacatataa
     7501 tgttgataca tgtggaggat ataattggtt cgaaaaatgg ctgaagctcc gaataattgt
     7561 cacaaaaatg cctgtctcag ttgttaaatc tataatagaa tcaacaacag gacaagcatc
     7621 tccatcaata ataatatgtg tcacaattat gcctctgtat tgttttcttt attttgttga
     7681 gaggcgcttt tggcaacata atctttatat tttttaaatg acttgatgcg tgctttatca
     7741 gcttcttgtt ggcgtttttg ttcttctttg tgtcgttttt caatattttt ttgtaacttt
     7801 ttattcattt tagcgatttc tttgcgattt ttttcagcta gtttatcgcc ttttttctca
     7861 gttttctcat ctaatttatt aggtgttaag cctgcttttt cttcgtattt ttgtgatttt
     7921 ttcatatctt taatacgttg tatttcattc ttttcgcggg ctttttgctc ttctttatga
     7981 cgcttttcga tatttttttg aagtatttta ttcattttat cagcgtcttt acgattttgt
     8041 ttagctaatt tttcgccttt tttctcaata taggcaggat catgttctct agcaaacttt
     8101 ttaagttcac gtttattttc aaaatcttgt tttttatcgc cgacatattc tttaacatca
     8161 ctcgctgtgt tactgattgc tgcagatgtt tttgaagcaa ctttacttgt agcatctgta
     8221 actttttgta cgtccggatg ttgtttgata cgtttacgtt caacaattaa cggtaccaat
     8281 acaattggta atacattaat cataaatttg atgacttttt tcttatccat agatcttgcc
     8341 tccataatta ctttattaat tttacatacc ctatgataca tcaatataaa cgatgatagt
     8401 agtgaatcac tattaagtat ttcagatgtt ttttaaaaga agacaataaa aactgccaat
     8461 caagtgattc cttaattgac agtctatatt ttaaaaggaa attaaatacc tttaccaatg
     8521 ccaaatccga agtaaagtat agcaataaag attactaata caattctgta aatggcaaat
     8581 ggaattagtt tgattttgtt aattagatgc aagaatgttt tgattgcaat tagtccaaca
     8641 gtaaatgcag ctaaaaagcc taaaatataa aaaggtatat cagcaatctg aatatcttga
     8701 taatgtttta ataaagataa accactagct gctaacataa ttggaacagc cataataaat
     8761 gtaaagtccg atgctgcttt atgatttaat ttcattaata ccccagttga aattgttgag
     8821 cctgaacggc tgaaaccagg ccacatagct actgcttgag aaataccaat tacaaatgct
     8881 tggaaataac tgatttgatc tactgtttgt gggtttttaa ctttagctga gtatttatca
     8941 gcaataatca tatagatagc acctacgaat aagccaatca taacagttgg cacactaaat
     9001 aaatgttctt cgatgaaatc atcaaatagt aagcctaaaa tacctgctgg caccataccc
     9061 actaatacat gtaataaatt taaacgtctt ggctttgaac gtctttgttg atcgttatct
     9121 ccttcaacat gtttgtgttt accaatatgt aaaatctcta agaagcgttc gcggaacacc
     9181 catgctgctg caaagacgga tcctaattgg atgacgattt taaatgtaaa tgctgactga
     9241 gaacctaaaa attcagatga ttttaaccac atatcatcaa ctaggatcat atgtccagta
     9301 gaggaaacag gtgcaaattc tgttaatcct tcgacgaccc ctaagataat accttttatt
     9361 aattcaatga taaacataat gtacccactt tcattactca atttaattta tttaaatatc
     9421 aaaattacca tatcatgata gcatattcat ttaaagac
//
LOCUS       DAKTLH010000098         9456 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.098, whole genome shotgun sequence.
ACCESSION   DAKTLH010000098 DAKTLH010000000
VERSION     DAKTLH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.098"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(50..565)
                     /locus_tag="O0394_001931"
     CDS             complement(50..565)
                     /locus_tag="O0394_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0500003.1"
                     /translation="MAGIIKEISEQNASELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            756..1760
                     /locus_tag="O0394_001932"
     CDS             756..1760
                     /locus_tag="O0394_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0500004.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            complement(1806..2207)
                     /locus_tag="O0394_001933"
     CDS             complement(1806..2207)
                     /locus_tag="O0394_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0500005.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDIAVLKSYQGQGYGSLIMEHIMQYIKGVAVESTYV
                     SLIADYPADKLYTKFGFIPTEPDSGGMYIKY"
     gene            complement(2243..3277)
                     /locus_tag="O0394_001934"
     CDS             complement(2243..3277)
                     /locus_tag="O0394_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0500006.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDRHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            complement(3288..3404)
                     /locus_tag="O0394_001935"
     CDS             complement(3288..3404)
                     /locus_tag="O0394_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500007.1"
                     /translation="MANYAIYFNNININSIMALNDNNYQFEHVNERNEYRES"
     gene            complement(3509..3940)
                     /locus_tag="O0394_001936"
     CDS             complement(3509..3940)
                     /locus_tag="O0394_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HCY0500008.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            complement(4147..5376)
                     /locus_tag="O0394_001937"
     CDS             complement(4147..5376)
                     /locus_tag="O0394_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCY0500009.1"
                     /translation="MIILVIFSLAFYPAYNPKPKDLPIGILNEDKGTTIQDKNVNIGK
                     KLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDKDFSKDAMSKTQKVVMD
                     SKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTIVSEGSSLQGSQIASAV
                     LTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVDNEKLNKVKDHQAGGNA
                     PFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQMIFAVVAAFAGSFVYIY
                     FMQGVQRFDFDHPNRIAIFVAFAILGFVGLILGVMVWLGMKSVPIFFILMFFSMQLVT
                     LPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNSTMWMFIGFMIFGAVSSL
                     VSAIVRKHSTKRTEVPS"
     gene            5581..6204
                     /locus_tag="O0394_001938"
     CDS             5581..6204
                     /locus_tag="O0394_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0500010.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGVPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIIFNGPLRIMAENRLHKSNLDSLT"
     gene            6250..7194
                     /locus_tag="O0394_001939"
     CDS             6250..7194
                     /locus_tag="O0394_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCY0500011.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(7280..8722)
                     /locus_tag="O0394_001940"
     CDS             complement(7280..8722)
                     /locus_tag="O0394_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HCY0500012.1"
                     /translation="MNYKQSAEEILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIIAITVTLILSKRKANKEVVE"
     gene            8905..9288
                     /locus_tag="O0394_001941"
     CDS             8905..9288
                     /locus_tag="O0394_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCY0500013.1"
                     /translation="MKERGYIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDVALLIEPSIGSVCNAFDHMWGYLKKCANEEERQQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
BASE COUNT         3209 a         1723 c         1365 g         3159 t
ORIGIN      
        1 cacaatattt attactatga tttcatttta tctgctgcaa aaacaatcat tataactctt
       61 tttccataat taaatctgta tccgttacat cacctgtttg aaaatgatgt tcaccaacca
      121 ctttaaatcc atgacgttta taaaatgctt gagcacgagg attatgctcc caaactccta
      181 gccaaatttt atgtttatta tgttcttgag caattttttc ggccaattct atcaattgtg
      241 aacctcttcc gccaccttga aagtctttca aaaaatatat gcgctgcact tctaaatagg
      301 tctcccccat ttcttcagtt tgagcactat taatattcat ctttatataa ccaacattcg
      361 caccatcttc ttgataaaaa taatgaaatg aatctacatg gttaatctct tgtgtaaatt
      421 tctctacagt ataattgtct ttaaaaaatt gatcaaaatc tttgtcatca tagtaagaac
      481 caaacgtgtc ataaaatgtt ctagttgcta attcaactaa ttcactagca ttttgttctg
      541 aaatttcttt gattatccca gccatataaa tcctccaata aacagtgatc gaatcaaaat
      601 attacttatg ttatttttca gccaaaacta tttaaaaata cattaacaca aatcaattac
      661 aaattgtatt gattgtgtgt aacatcaata aatgatacat ttattccagt aaaatggccg
      721 tattttcaaa agagaaaaag agaggatgta tcgttgtgat agaaacattt aaagcgtttg
      781 taattgataa agatgagagt ggtaaagtga caccaacttt caaacaatta tcgcctactg
      841 atttacctaa aggagatgtg ctgattaaag tacattactc tggtataaat tataaagatg
      901 ctttagcgac tcaagatcat aatgcagtcg taaaatcgta tcctatgatt ccaggaatag
      961 atttagctgg aacaattgtt gaatccgaag caccaggctt tgaaaaagga gaacaagtaa
     1021 ttgtaacgag ttatgaccta ggtgtcagcc attatggcgg ttttagtgaa tatgcgcgtg
     1081 taaaatcaga atggattatc aagcttcctg atactttaac attagaagaa tcaatgatat
     1141 atggcacagc tggttatact gccggtttag caattgaaag acttgaaaaa gttggaatga
     1201 atattgaaga tggtcctgta ctcgttcgcg gtgcttcagg tggtgtcggt actttagcag
     1261 tactcatgct taatgaactt ggttataaag ttatcgcaag tacaggtaaa caagatgtta
     1321 gcgatcaatt acttgaactt ggtgccaaag aagttatcga tcgacttcct gttgaagatg
     1381 atcataaaaa gccactcgca tcatcaactt ggcaagcttg tgtagaccct gttggtggcg
     1441 aaggtattaa ttatgttaca aagcgtttaa atcatagtgg gtcaattgca gttattggta
     1501 tgactgccgg taatacttat actaattctg tattccctca cattttaaga ggtgtaaaca
     1561 ttttaggaat tgactcggta tttactgcta tgaaattaag acagcgcgtt tggcgtcgtc
     1621 tcgcaaaaga tttaatgcct gaaaatttac atgagatcaa gcaagttatt acatttgatg
     1681 aacttccaga acaacttaac aaagtaatta aacatgaaaa taaagggcgc attgttatcg
     1741 atttcggtgt agataaatag tattcatgaa aaagacatcc cgttatgcga gatgtctttt
     1801 ttaatttagt atttgatata cataccgcct gaatctggtt cggtaggtat aaatccaaat
     1861 tttgtatata atttatccgc tgggtagtct gcaatcagac taacgtatgt actctcaaca
     1921 gccacacctt taatatattg cataatatgc tccataatta gactgccgta accttgacct
     1981 tggtaacttt tcaaaactgc aatatcaaca atttgaaaaa cagttccgcc atcgccaatc
     2041 actctaccca taccaattaa ccgatcttta tcatacaagg ttactgtaaa taaggcatta
     2101 ggtaatcctt tttcagctgc ttcgcgcgtc tttggactca tacctgcgtt aatccttaat
     2161 gcgcaataat cctcgcaagt cggaatatca tatgtcactt taaccattat ttaccccact
     2221 tttcatcaca caatatatca acctagtata aatgtttatt tacaatagtc ttattcgctt
     2281 ctttaaacac ttcatgatga cttgaaacat aaccctctgc attcgcatct ggttggatat
     2341 atgttttagc aaggttcgct gcatttgcac catcactaaa tgcacttgca attagatgtg
     2401 attttgcatc atgataaaca atatctccac acgcatagat accaggtata ctagttgtcg
     2461 tattaccaaa tcctttaaca cgacaatcat catgcatatc tagctttgaa gatgtttcac
     2521 tcaataatgt attacaacga tcaaacccat gactaataat gacatcgtca aatttaactg
     2581 tatgcctatc gccactttca acatgttcca aaacaacttc acttatatgc gtttcatcat
     2641 cattgccgac caagtattta atacgtgttt ttgggcatag tttcacattt aaatctgtca
     2701 ccaacgtttt catcgcttca tgaccactta catcttcttt tcgataaaca actgtcacgc
     2761 ttttagcaat cttggcaata tcatgcgccc aatctaatgc tgtatttcct ccacctgata
     2821 ttaatacatc tttatctttg aaacgtctgt aactttgtac aacataatgt aaattagtta
     2881 attgatatct ctctacacct ttaacatcta attgttttgg attaataata cccgcaccaa
     2941 ttgcaatgat aactgctttc gatgtatata tttctcccgc ttctgtttca acttcgaaat
     3001 gacgttctgc ctttttccta atatctacca cacgttcatt caaatgaact tccggtttaa
     3061 aatataatcc ttgcttaatt gtatctttta aaatttcatg acaaggtttt ggcgcaatgc
     3121 cgccaatatc ccaaataatt ttttcagggt aaattctcat cttaccccct aattcagatt
     3181 gaacatctat caatcttaca gacatatctc gcaatccagc ataaaagctt gcatacaaac
     3241 cagacggacc gccaccaatg attgtaacat ctttcattat gtgcctccta tgactctcta
     3301 tattcatttc tttcattaac gtgctcaaat tgataattat tatcatttaa agccattata
     3361 ctattaatat ttatattgtt aaaataaatc gcatagttag ccatgaatta tcaataacta
     3421 tcccatcaca atataaaaac acacctttca aagtaagaaa caacaatgtt cctttgaaag
     3481 atgtgtatat tttttagttc tagttatatt attttttaaa agactcatca cgtggttctt
     3541 taagaattgc ttgtcttaaa aggaaaaata gcaacaataa acctgcaagc atacctgtgt
     3601 gcccaatacc tgcaaagcct gcaaatgctt ctggagagta tgatttacca gtgacttgga
     3661 agaatccttt tgtcaccatc atacctatag taacgataac acctatgtta tatacataga
     3721 agaaccagtt aaataagtag taacttgata atttaaataa cttctccaat ggtaataata
     3781 ttaaaaacat aaacatacct aaaataagtg tatgtgtgtg tacaagtgat aattgtgttg
     3841 taccagtaaa atcatatgct tttgttagct ctctatagaa aaagccacta aataaaccta
     3901 tgatcatata aaaaagaaat gaatataata acctacgcat tttgaatcct ccctcacctt
     3961 ttcattatat ccgattatac ctattataac agtgacaaaa aagcgaagat atgacacaac
     4021 tgaatttggt ttagcacata gtatgacagc taaaataact ttacatcaag cgctatacaa
     4081 ataaaatagc tataccatga cgctcgctac aaacgtcttg tatagctatt atttctattt
     4141 attaaattat gacggcactt cagtacgttt tgtgctatgt tttctaacaa tagctgaaac
     4201 taaacttgat acagcgccaa aaatcataaa ccctataaac atccacattg tactatttaa
     4261 ttcaatatga tgattcaagt ataatagttc tcttacactt gttgcatagt gtgtgaatgg
     4321 attccaatca tatacatatt tttgataact ttcaggcaac atttgtttag gtaacgttac
     4381 aagttgcata ctaaagaaca ttaaaatgaa gaaaattgga actgacttca tacctagcca
     4441 taccataaca cctaaaataa ggcccacgaa accaagaatc gcaaatgcta caaaaattgc
     4501 gatacgattt ggatggtcaa aatcaaatct ttgaacgcct tgcatgaaat aaatataaac
     4561 aaagctacct gcaaatgctg caacaactgc aaatatcatc tgtccaattg aagcaatgat
     4621 acgatgttgc acgacgatat tgttactagt tctaaatgca aagaacaata agatagacgt
     4681 tacgattgaa cctatccaaa ttggcataaa cattaggaat ggtgcattac cacctgcttg
     4741 gtgatcttta actttattaa gtttttcatt atccactttt actggattcg tcaaaccatt
     4801 gatgtccgcg gcattgactt taacattttg actcgttaat gtttccaaac tttgcttcgt
     4861 aatttgagca ttaatattat cacccatacc agttaacaca gctgatgcaa tttgtgaacc
     4921 ttgtaagctt gatccttcac ttacaatcgt tttaaattta gcctgcttaa cctctacttg
     4981 ttgattcccc attttttgtt tcatttgttg aaccacttgt ggcggaattt cacctgaagc
     5041 aactttttgt tgcatttctt cttttttact atccataact actttttgtg ttttactcat
     5101 agcatctttt gaaaagtctt tatcaataat agctactcca aagatttttt gatctttcaa
     5161 atctttttca aggtcttttt cactatcaac cttaacccat ttaattttat tagaatcact
     5221 atctaataat ttatcctcta attttttacc aatgttaaca tttttatctt gaatcgttgt
     5281 acctttatcc tcgtttaata taccaattgg taaatctttt ggtttaggat tatatgcagg
     5341 ataaaaagct aaagaaaaga ttaccaagat aatcatagtt gctattggtg caatccataa
     5401 taatttttaa atatattcat tttattaatt acctccttat tagacaatgt gttgaatatt
     5461 tcgcatgttg tctattataa tatgtaagtg ggaataaaca atacacaatg ttcatcaata
     5521 cgttcattaa tagacaaata cacccacatt gttcaataac acaaggaggt tgatttattt
     5581 ttgaaagaag ataggcgaat tagaaagaca aagtcctcca ttaaacaagc gttcactaaa
     5641 ttactacaag aaaaagattt agaaaagatt accattcgcg acataacaac acgcgctgat
     5701 atcaatagag ggacatttta cttacattac gaggataaat atatgttact cgcagatatg
     5761 gaagatgagt atatttcaga actaacgaca tatactcaat ttgatttgtt acgcggttct
     5821 tcaattgaag acattgcgaa tacttttgta aataatatac tcaaaaatat tttccaacat
     5881 attcatgata atttagagtt ttatcatact attttacaat tggaacgcac gagtcaatta
     5941 gaattgaaaa tcaacgaaca tattaaaaat aatatgcaac gttacattag tatcaatcat
     6001 tctatcggag gcgttccaga gatgtatttt tacagttacg tttccggagc aacaatttca
     6061 attattaaat actgggtaat ggacaaacag cccatttcag ttgatgaatt agctaaacat
     6121 gtgcataata ttatttttaa tggtccatta agaataatgg cagaaaaccg tttgcataaa
     6181 tcaaaccttg attcactaac ttaagtaata ctcgcttttc ctaaatcctt tagaaaggaa
     6241 ctatatatca tgattacttc attcagacat tctgaagata tagataaaca tattataaaa
     6301 acaccattag atcatacagc gtcatggatt aatgtagtag aaccagaccg agaagaaatt
     6361 gaaaatctta tggaacaata taatatacct gaagacttta tacgtgaccc tttggactca
     6421 gaagaaagtt cccgtattga atatgacgaa gatactggtt actcattaat cattattgat
     6481 ttacctatcg tcaattcaac taatcgtagc gttctatctt ttgtaacgat tccattaggc
     6541 attattattg gcaacggtat tatcgtaaca gtttgtgacg ctgaaaatga atttttagaa
     6601 aatttaccta agcgtgatat taatttaaaa tttcacagca gatttgcatt agaaatttta
     6661 actactattg ccgaccatta taatcgtaac ttacgattac ttaataaaag tagaattcgt
     6721 attgagaaag aactaaaaaa taacattact aacaaacaac ttttcaaatt aatggaagtt
     6781 gaaaaaagtt tagtatactt tttagctgcc ttaaaaggta acgatacaat tattaaaaag
     6841 ttattccgtt tacctgcaat taaacgtttt gaagaagatg aagaattact agaagattta
     6901 atcattgaaa ataaccaagc catcgaaaca acagaattac atcaacgtat cttagaaagt
     6961 atcacaacat catacgcttc cttattatct aatgatatga atacgattat gaagacatta
     7021 acacttttca cggtactatt aacgttacca acactcgtat ttagtttctt cggtatgaat
     7081 gtgccgttgc caattgatga ccatagttat atatcttgga ttattgtggt aggtatttca
     7141 ttaattcttg tcgtaattgt tagtattttc ttatggcgta aacagaaatt ataactaaat
     7201 taaaaggtat gacagcttaa agtttcatcc tattgcattt gcaatgactg atactttgat
     7261 tactgtcata cctctttttt tattctacaa cttctttatt tgcttttctt ttagataaaa
     7321 ttaaagtaac tgttatagca atgatgaatg atattgtcat accaacaaag tagtgtaacc
     7381 atcctgcatg tactggattg attgaaataa atcctggcaa tccagcagtt cctaatgcga
     7441 ttgctttaac cttgaagaaa gcaatatatg ctgaaccaat acctgatcca acgatagcgc
     7501 caataaatgg atatcttagt tttaagttaa caccaaacat agccggttct gtaataccaa
     7561 gtaatgctga aatacctgcg gcagatgcca caccttttaa cttcttattt tgtttaataa
     7621 taaagaacgc tgcaattgct gcaccacctt gtgcaacatt agacattgtc gcaatcggga
     7681 atataaatga tccacccgtt ttagtggcat ctgcaattaa tgtcgtttct acagctataa
     7741 agctatgatg catacctgta ataacaatcg gagcatacaa taatccgaat attaatccac
     7801 caattgcacc accaaattca taaagccatg ttaaaccatc tgataaccag taacctaatt
     7861 gtcgagtgat tggacctaca aatgaaaatg ttagaaatgc tgttataaaa atagataaca
     7921 atggtgttaa caaattatct aacaccgttg gaataacttt gcgtaaccct ttttcaattg
     7981 aggctaagat ataagctgct acaagcatag gtaacacttg tccttgataa cctacttgat
     8041 taatatgcaa accaaaaaca tcccaatatg gaatagcctt acctgcttca actgcttttg
     8101 ggaaatcgta tgcgctcatt agcgatggat gaactagtat catacctaat gcagcaccta
     8161 aaaatggatt gccaccaaag cgttttgctg cactaaatcc aattaaaatt ggtaataatg
     8221 taaatggtgc attcgcaaaa acatttatca tttcagctaa tccagcaaat tgactatata
     8281 catctatcaa tgatttacct gaaaagaata aatctttcgc agtcaaaatg ttatttaacc
     8341 ccattaataa accaccagca acgatggctg gtataatcgg aacaaagata tctgaaagca
     8401 tttttacaaa tcgctgtaac ggattcatat ttttagcaga ttgtgctttg acttccgaag
     8461 tggttgatgc ttcttttcca gttaattttt ccagttcact aaatacttta ttgactgtac
     8521 cagacccaat aataatttgg tattgtcccc cagtagaaaa cgtcccttta actacatcca
     8581 tattgtttag cgcctcttca tttactaaac tttcatcatt taaaactaat cgtagtctcg
     8641 ttgcacaatg cgccattgca tctaaattct cttctccgcc tatcgcgttc aaaatttctt
     8701 ctgcggattg cttataattc atatatttag cctcctaatt agaaccggtt ccatttattc
     8761 cactttattg taaccggttc caaaaatatt tgcaacactc tattatcatt ttatataaac
     8821 acctacacca tttttgatag cgcattcatt tttattgcta tactacatgc atgtatatta
     8881 acattaatta tcggaggtaa ttttatgaaa gaacgtggct atatcgaaca gctttggcgt
     8941 gaggaaaaat atcatgtatt gttacacagt caacagtctt accaaatgat aaggaatgct
     9001 ttaaaaacgg atttatcact tcatcaagtt caacaaatga ttgatgttgc tttgttaatt
     9061 gaaccgagta taggaagtgt atgtaatgct tttgatcata tgtggggata tttaaaaaaa
     9121 tgtgcgaatg aagaagaaag acaacaatca aaactactta aagctgattt tattaatggt
     9181 aaaatcgaca cccaaacttt attagatttt ttagcagaac ttgctaataa atatgatgtt
     9241 caatatttac tacaaagtcg tgttttaaac acaaaaagaa agagatagat tgaaacttta
     9301 gttgtaacgg acaaatagta gtcattggag ttcagcaatt catactttgt actaaagttt
     9361 actataatct atctctcaag gtttaaatgt acatatctat attaaaatgc gtttcatgat
     9421 gttagaagat accatacaca tttaattagt tacagc
//
LOCUS       DAKTLH010000099         9453 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.099, whole genome shotgun sequence.
ACCESSION   DAKTLH010000099 DAKTLH010000000
VERSION     DAKTLH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9453
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.099"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(125..964)
                     /gene="noc"
                     /locus_tag="O0394_001942"
     CDS             complement(125..964)
                     /gene="noc"
                     /locus_tag="O0394_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HCY0500014.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(1007..1726)
                     /gene="rsmG"
                     /locus_tag="O0394_001943"
     CDS             complement(1007..1726)
                     /gene="rsmG"
                     /locus_tag="O0394_001943"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCY0500015.1"
                     /translation="MTVEWLAEQLKEHNIQLTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(1726..3603)
                     /gene="mnmG"
                     /locus_tag="O0394_001944"
     CDS             complement(1726..3603)
                     /gene="mnmG"
                     /locus_tag="O0394_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCY0500016.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILELLEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(3670..5049)
                     /gene="mnmE"
                     /locus_tag="O0394_001945"
     CDS             complement(3670..5049)
                     /gene="mnmE"
                     /locus_tag="O0394_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCY0500017.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVIEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(5188..5541)
                     /gene="rnpA"
                     /locus_tag="O0394_001946"
     CDS             complement(5188..5541)
                     /gene="rnpA"
                     /locus_tag="O0394_001946"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCY0500018.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(5662..5799)
                     /gene="rpmH"
                     /locus_tag="O0394_001947"
     CDS             complement(5662..5799)
                     /gene="rpmH"
                     /locus_tag="O0394_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCY0500019.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            6530..7891
                     /gene="dnaA"
                     /locus_tag="O0394_001948"
     CDS             6530..7891
                     /gene="dnaA"
                     /locus_tag="O0394_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCY0500020.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            8169..9302
                     /gene="dnaN"
                     /locus_tag="O0394_001949"
     CDS             8169..9302
                     /gene="dnaN"
                     /locus_tag="O0394_001949"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCY0500021.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
BASE COUNT         3024 a         1742 c         1390 g         3297 t
ORIGIN      
        1 ccagcctgat caacatataa atataaattt ttatgtttca cgtaaaacaa aaaaaaagac
       61 aaagctgtta tgatcttagc tttgtctttt atgtgttagt catcatgtat acgacatcct
      121 actactaacg tttatatatt cgaattttta tttcataata atcttcatga tctttgtctt
      181 tatgctcaac atgtaatcct gtttgttgaa tcgcttggat acttttgcct acctcatctc
      241 gtgcttgagt gacatcttgt gcaaagcgaa ggttttgtgc tttgactttt tcgggccccg
      301 ttttttggcg tactctatct tcagtctgtt tcacatttag cttttgtgca atgacttgct
      361 caatcaacgc ttcttgttcg ctatcagaca atgataatac cgctctcgca tgacgttcag
      421 taattttacc ttctcttaag cgaagtagta ctttcggcgc caacttcaat aaacgcaact
      481 tatttgcaat aaagctttga cttttaccta aactttttgc caattcactt tgcgttgtat
      541 caccaatttc caataatttc ttataggctt ccgcttcttc aacaacagac aaattttctc
      601 tttgaatatt ctcaattaat gcaacaacag ccgtctcttc atcatccata tcacgaataa
      661 taacgtctgc ttgaggtaaa tttagtgatt gtattgctct aaatcggcgc tctccagcaa
      721 taatttcaaa catatcttct tcaatcggtc ttacaacaat aggttgtagt aaaccatgtt
      781 catgtattga ttcagcaagt tctttaattt tatttggttc aaacacctgt cttggttgat
      841 aacggttggg aacgatacgt tcaatttgaa tggattcaac attactattg cgatcttctt
      901 caatatgtcc aatgatgtca tctttgtttt tcaaaccaaa taattttgaa aaaggttttt
      961 tcattatcca ttcgctcctt ttatgttagt tgtaaaggat tatgcattat ttttcaagta
     1021 aaggagtctt attaggcgtc cctggttttc ttggatactt tttcggcgtc tgtctttttt
     1081 tatcaataat gaacatctgg cgctctccag catcttctgg caattcaaag gtatgtgttt
     1141 ctgtaacatt accacctaac acactaattg caaattttgc ttcttctaat tcttcttcac
     1201 cttttgaaga ttttaatgca acaaactgtc cacctttttt aactagcggt aaacacaatt
     1261 cacttaacac ggataatcta gctactgctc ttgcagtaac aacatcataa gactccctgt
     1321 agacaccctt accaaatgtt tctgctctat cgtgtataaa gctgacatcc tgtaattgta
     1381 attctgacgc taaatggttt aaaaattgaa tacgcttatt taatgaatca acaatcgtca
     1441 cttttaactg cggaaacatt atttttaacg gaatacttgg aaaaccagct ccagcgccta
     1501 catcacatat acttataggc tgattaaaat caaaataaaa actaggtgca atggaatcat
     1561 aaaaatgttt caaatataca tcgtgttcat ctgtaatact tgtcaaattc atcttttcat
     1621 tccattcaac aagtaaacga taatatgttt gaaactgttg tttttgagtc tcagttaatt
     1681 gaatattatg ttcttttaat tgttctgcta accattctac agtcattagt cactcaccct
     1741 ttggagttta ccttgttcta agtaaatcaa taatatagat atgtctgctg gatttacccc
     1801 tgatattcta gaagcttgtg caatatttaa aggttttact tctgataatt tttctcgcgc
     1861 ttcagtcgcc aaactatcaa tcttactata atctaagtct tctggaattt tcttctcttc
     1921 catacgctta actttctcaa cttgttgtag tgatttattg atataacctt catattttgt
     1981 ttgtatttct acttgttctt caacatctgc attcaattga tgttcttctt ctaaaagttc
     2041 taaaattata tcgtaagtca tttcaggtct gcgtaataaa tcgatagcta aaataccatc
     2101 ttttaagcga gaaccaccat gttgttcaat aatcgcttgc gtatgttcgt ttggtttaat
     2161 acgaatatct gataaacgct taatttccgc atcaatttgc tgacgttttt cattaaaacg
     2221 tgcatatctt tcttcagaaa tcataccaag ttcatatccc atatccgtca atctcaaatc
     2281 agcattatca tgacgtagta acaaacgata ttctgcacgt gatgttagta aacgataagg
     2341 ttcattagta cctttagtta caagatcatc gattaagaca ccaatatatg catctgaacg
     2401 acttaatatc ttttcgcctg tgtttaacac tttacctgca gcgttaatac ctgccatcaa
     2461 tccttgtcct gctgcttctt cataaccaga tgtaccatta atttgacctg cagtatataa
     2521 gtttttaatc attttcgttt caagtgtagg ccataactgc gttggcacaa tcgcatcata
     2581 ttcaattgcg tagccggcac gcatcatatc tgctttttca agacctggta tcgtctctaa
     2641 catttgacgt tgcacatgtt caggaagact tgtagacaat ccttgcacat atacttcatt
     2701 tgtattacga ccttcaggct ctaagaaaag ttgatgtcgc ggcttatcat taaatcgaac
     2761 aaatttatct tcaattgaag ggcaataacg tggcccggtt cctttaatca tccctgaata
     2821 cattgcagat agatgtaaat tatcatcgat aactttgtgt gtttcagcat tagtatacgt
     2881 tagccaacat ggcaattgat ctaatatata ttctgttgtt tcaaagctga atgcacgacc
     2941 tacatcgtca cctggttgta tttcagtctt cgaatagtca attgtttttg aatttacacg
     3001 cggtggtgta cctgttttaa aacgaacaat atcaaaacca agttctctta aattgtctga
     3061 taatgtgatt gatggtaatt ggtgatttgg tccacttgaa tacttcatat tacctaaaat
     3121 gatttcacca cgtaaaaatg ttcccgttgt aataattact gctttagata aatactctgt
     3181 accaatattt gtacgtacac ctttaacttc attatcttct ataataagtt cgtctaccat
     3241 accttgcatt atatgcaaat tttcttcatc ttcaatcacg cgtttcattt cttgttgata
     3301 aagtacttta tctgcttgcg ctcttagtgc tcttacagca ggtcctttac ctgtatttaa
     3361 cattctcatt tgaatgtgtg ttttatcgat tgtttttgcc atttgtccac ctaaagcatc
     3421 aatttcacga acaacgatac ctttagctgg tccacctaca gatgggttac atggcataaa
     3481 tgcaatatta tctaaattta ttgttagcat taatgtttta gcaccacgtc ttgcagatgc
     3541 taaacctgct tctacacctg catgtcccgc acctataacg attacatcat attcttgaac
     3601 cacaatataa acctccttat ttgatatctt actagccttc ttaagacggt attccgtcta
     3661 tttcaattac tatttaccta agcagaattg actgaataac tgatcgatga gttcatcact
     3721 tgcagtctca ccaataattt ctcctaatat ttcccaagtt ctagttaaat caatttgtac
     3781 catatccata ggcacaccag attctgctgc atcaatcgca tcttgtatcg tttgtcttgc
     3841 ttgttttaat aatgaaatat gtcttgaatt agaaacataa gtcatatctt gattttgtac
     3901 ttctccacca aagaacaaat ctcgaatttg tatttctaat tcatcaatac cttcttgttt
     3961 taacattgaa gtttgaatta atggcgtatc acctatcata tctttaactt cattaatatc
     4021 tatgttttgc tctaaatcca ttttattaac aattacgatt acatcttcat ttttaaccac
     4081 ttcatataat gtgtaatctt cttgagtcaa tgcttcgtta ttgtttaata caaataaaat
     4141 taagtctgct tggctaagag cctttctaga gcgttcaaca ccaatcttct ctactatatc
     4201 ttctgtctca cgtataccag cagtatcaac taatcttaat ggcacgccac gaacattgac
     4261 gtactcttct aagacatctc tagtagtacc tgctacctca gttacaatcg ctttattatc
     4321 ttgtattaaa ttatttaaca tcgatgattt acctacgttt ggtttaccaa caataactgt
     4381 agataaacct tcacgcataa ttttaccctg cgcaccggta tctaataaac gattaatttc
     4441 ctgtttgatt tctttagact gctctaaaag aaattcagta gtcgcatctt caacatcatc
     4501 gtattcagga taatcaatat tcacttccac ttgagcgagt atctctaata tagattgacg
     4561 ttgttttttg attaagtcac ttagacgacc ttcaatttga ttcatcgcaa ctttagaagc
     4621 tctatctgtc ttcgagcgaa taaagtccat aactgcttca gcttgagata aatcaatacg
     4681 accatttaaa aaggcacgtt ttgtaaattc acctggctca gccattctag cgccatatgt
     4741 catagtaagt tccagcactc tattaatcgt taaaatacca ccatgacaat taatttctat
     4801 aatatcttcg cgtgtaaatg tttttggcgc tcttaacaca gacaccataa cttcttcaat
     4861 cacttcttta gactctggat caataatatg accgtaatta atcgtatgtg atggaacatc
     4921 atttaaaaga tgttttcctt tatataattt gtcagcaatt tcaacggctt gcggtccaga
     4981 caatcgaaca attccaattg ccccttcacc cattggtgtt gaaatactcg taattgtatc
     5041 taaatccata ttgctactcg cctccttcaa cgatgtgaat acattttaaa gtaagttatt
     5101 ataaccctaa ggtcagtctt acgtttgtct gaggtaagac ttcgggatgt gttgagtggt
     5161 taatgttttc cttcccctac cctatcctta cttaatcttt ttattaaaaa ctttggcaat
     5221 tttaagtacg tgctcaagac tattctgtat ttgtaaagtc gtcatatctt tagctggctg
     5281 tcttgctatt acaataatat ctttggccaa tatatgcgac ttatgtactt tgaaattttc
     5341 acgtattgct cttttaatct tgtttcttaa cactgcatta cctagttttt tagaaacact
     5401 aatacctaag cgaaaatggt ctatttcttt attattacaa gtgtatacaa caaattgtct
     5461 gttggctaca gaatgacctt ttttatatat tctctgaaaa tctgcattct ttttaattcg
     5521 gtaagctttt tccaataaca tcactcgctt atttatcgtt tttatttgaa gctatattta
     5581 aacttctatt gagcttataa cataaatttc tatttattct taatttaaac gaaaaaaaag
     5641 atcactgatg agtcagtgat cttatgcaga taaaacttta cggcctttac gacgacggcg
     5701 cgctaaaact ttacggccat tttttgtgct catgcgtttt ctgaaaccat gaactttact
     5761 atgtttacgt ttatttggtt gataagtacg ttttaccatg caaaacacct ccatctttat
     5821 acagttatct aatataccag attatgcaga ctctgatcac tttaatagat aagattttgc
     5881 gtttcttaga ttaaaattct cgtattagct cattgattat ctagtcataa ttcaagcaac
     5941 tactacaata taacaaaatc ctatttataa cgcaagttca ttttatacta ctgctcaatt
     6001 tttttacttt tatcgattaa agatagaaat acacgatgcg agcaatcaaa tttcataaca
     6061 tcaccatgag tttggtccga agcatgagtg tttacaatgt tcgaacacct tatacagttc
     6121 ttatacatac tttataaatt atttcccaaa ctgttttgat acactcacta acagatactc
     6181 tatagaagga aaagttatcc acttatgcac atttatagtt ttcagaattg tggataatta
     6241 gaaattacac acaaagttat actattttta gcaacatatt cacaggtatt tgacatatag
     6301 aggactgaaa aagtataatt gtgtggataa gtcgtccaac tcatgatttt ataaggattt
     6361 atttattgat ttttacataa aaatactgtg cataactaat aagcaagata aagttatcca
     6421 ccgattgtta ttaacttgtg gataattatt aacatggtgt gtttagaagt tatccacggc
     6481 tgttattttt gtgtataact taaaaattta agaaagatgg agtaaattta tgtcggaaaa
     6541 agaaatttgg gaaaaagtgc ttgaaattgc tcaagaaaaa ttatcagctg taagttactc
     6601 aactttccta aaagatactg agctttacac gattaaagat ggtgaagcta tcgtattatc
     6661 gagtattcct tttaatgcaa attggttaaa tcaacaatat gctgaaatta tccaagcaat
     6721 cttatttgat gttgtaggct atgaagttaa acctcacttt attactactg aagaattagc
     6781 aaattatagt aataatgaaa ctgctactcc aaaagaaaca acaaaacctt ctactgaaac
     6841 aactgaggat aatcatgtgc ttggtagaga gcaattcaat gcccataaca catttgacac
     6901 ttttgtaatc ggacccggta accgctttcc acatgcagcg agtttagctg tggccgaagc
     6961 accagccaaa gcgtacaatc cattatttat ctatggaggt gttggtttag gaaaaaccca
     7021 tttaatgcat gccattggtc atcatgtttt agataataat ccagatgcca aagtgattta
     7081 cacatcaagt gaaaaattca caaatgaatt tattaaatca attcgtgata acgaaggtga
     7141 agctttcaga gaaagatatc gtaatatcga cgtcttatta atcgatgata ttcagttcat
     7201 acaaaacaag gtacaaacac aagaagaatt tttctatact tttaatgaat tgcatcagaa
     7261 taacaagcaa atagttattt cgagtgatcg accaccaaag gaaattgcac aattagaaga
     7321 ccgattacgt tcacgctttg aatgggggct aattgttgat attacgccac cagattatga
     7381 aactcgaatg gcaattttgc agaagaaaat tgaagaagaa aaattagata ttccaccaga
     7441 agctttaaat tatatagcaa atcaaattca atctaatatt cgtgaattag aaggtgcatt
     7501 aacacgttta cttgcatatt cacaattatt aggaaaacca attacaactg aattaactgc
     7561 tgaagcttta aaagatatca ttcaagcacc aaaatctaaa aagattacca tccaagatat
     7621 tcaaaaaatt gtaggccagt actataatgt tagaattgaa gatttcagtg caaaaaaacg
     7681 tacaaagtca attgcatatc cgcgtcaaat agctatgtac ttgtctagag agcttacaga
     7741 tttctcatta cctaaaattg gtgaagaatt tggtgggcgt gatcatacga ccgtcattca
     7801 tgctcatgaa aaaatatcta aagatttaaa agaagatcct atttttaaac aagaagtaga
     7861 gaatcttgaa aaagaaataa gaaatgtata agtaggaaac tttgggaaat gtaatctgtt
     7921 atataacagc actaatgata acaatcattt tttacatttc tatatgctaa tgtggcaaga
     7981 tgagcaaaac tcattttgtg gataatgttt aaaagtcata cacaccatac acaagttatc
     8041 aacatgtgta taacttcgcc aaatctatgt ttttaagact tatccaccaa tccacagcac
     8101 ctactactat tactaagaac ttaaaaccta tataattata tataaacgac tggaaggagt
     8161 tttaattaat gatggaattc actattaaaa gagattattt tattacacaa ttaaatgaca
     8221 cattaaaagc tatttcacca agaacaacat tacctatatt aactggtatc aaaatcgatg
     8281 cgaaagaaca tgaagttata ttaactggtt cagactctga aatttcaata gaaatcacta
     8341 ttcctaaaac tgtagatggc gaagatattg tcaatatttc agaaacaggc tcagtagtac
     8401 ttcctggacg attctttgtt gatattataa aaaaattacc tggtaaagat gttaaattat
     8461 ctacaaatga acaattccag acattaatta catcaggtca ttctgaattt aatttaagtg
     8521 gcttagatcc agatcaatat cctttattac ctcaagtttc tagagatgac gcaattcaat
     8581 tgtcggtaaa agtgcttaaa aacgtgattg cacaaacaaa ttttgcagtg tccacctcag
     8641 aaacacgccc agtactaact ggtgtgaact ggcttataca agaaaatgaa ttaatatgca
     8701 cagcgactga ctcacaccgc ttggctgtaa gaaagttgca gttagaagat gtttctgaaa
     8761 acaaaaatgt catcattcca ggtaaggctt tagctgaatt aaataaaatt atgtctgaca
     8821 atgaagaaga cattgatatc ttctttgctt caaaccaagt tttatttaaa gttggaaatg
     8881 tgaactttat ttctcgatta ttagaaggac attatcctga tacaacacgt ttattccctg
     8941 aaaactatga aattaaatta agtatagaca atggggagtt ttatcatgcg attgatcgtg
     9001 cctctttatt agcgcgtgaa ggtggtaata acgttattaa attaagtaca ggtgatgacg
     9061 ttgttgaatt gtcttctaca tcaccagaaa ttggtactgt aaaagaagaa gttgatgcaa
     9121 acgatgttga aggtggtagc ctgaaaattt cattcaactc taaatatatg atggatgctt
     9181 taaaagcaat cgataatgat gaggttgaag ttgaattctt cggtacaatg aaaccattta
     9241 ttctaaaacc aaaaggtgac gactcggtaa cgcaattaat tttaccaatc agaacttact
     9301 aaaaataaat ataaataaag gatgacgtga ttaattaaaa cgtcatcctt tattttttgg
     9361 caaaaataat tctaggtgcg tatgtaaaat aaatttggca gcattttaaa cagcaaataa
     9421 aagacgccaa ttaaatttat gacaaatgta tcc
//
LOCUS       DAKTLH010000100         9410 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.100, whole genome shotgun sequence.
ACCESSION   DAKTLH010000100 DAKTLH010000000
VERSION     DAKTLH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9410
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.100"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            419..769
                     /locus_tag="O0394_001950"
     CDS             419..769
                     /locus_tag="O0394_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500022.1"
                     /translation="MKTITQELKQYITHLFQLSNNEAWECEALEEAAENILPERFIND
                     SPLVHLTLETYTYYNDELHELSIYPFLMYANNQLISVGYLDHFDMDFLYLTDTQNIII
                     DERHLLKQGGQDHE"
     gene            856..1167
                     /locus_tag="O0394_001951"
     CDS             856..1167
                     /locus_tag="O0394_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCY0500023.1"
                     /translation="MKINRYITRGINESIPLDLQILLWHMVEKKDNQPHTDYLHIFKL
                     QEDENILSITHEQEQPTYKLEYHYTNYEKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            1182..1688
                     /locus_tag="O0394_001952"
     CDS             1182..1688
                     /locus_tag="O0394_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCY0500024.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKTWDEKKAACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSERYGAVYLVNLFSNIRTPENLKHIKNPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEIMHPLNPKA
                     RQKWILKS"
     gene            1740..3347
                     /locus_tag="O0394_001953"
                     /pseudo
     CDS             1740..3347
                     /locus_tag="O0394_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(3338..3598)
                     /locus_tag="O0394_001954"
     CDS             complement(3338..3598)
                     /locus_tag="O0394_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCY0500025.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(3570..4556)
                     /locus_tag="O0394_001955"
     CDS             complement(3570..4556)
                     /locus_tag="O0394_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCY0500026.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            4653..6662
                     /gene="mecA"
                     /locus_tag="O0394_001956"
     CDS             4653..6662
                     /gene="mecA"
                     /locus_tag="O0394_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCY0500027.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(6708..7136)
                     /locus_tag="O0394_001957"
     CDS             complement(6708..7136)
                     /locus_tag="O0394_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCY0500028.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(7233..7976)
                     /locus_tag="O0394_001958"
     CDS             complement(7233..7976)
                     /locus_tag="O0394_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCY0500029.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
     gene            complement(8813..9184)
                     /locus_tag="O0394_001959"
                     /pseudo
     CDS             complement(8813..9184)
                     /locus_tag="O0394_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016912861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase family
                     protein"
     gene            9238..>9410
                     /locus_tag="O0394_001960"
     CDS             9238..>9410
                     /locus_tag="O0394_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCY0500030.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYTLSYRDISEILRERGVNVHH
                     STVYRWVQEYAPI"
BASE COUNT         3425 a         1546 c         1415 g         3024 t
ORIGIN      
        1 ctaatgaaac agttattgtc gtaatttttt cataactata caatgcttat attgaaattg
       61 ctaaagaaca tcccacaatg attttttatc gggaaatgat tgggatttgc agattcgaag
      121 aaacagaagg attgtgcaag cacaatccca aaatcccgtt actttttata aataaataat
      181 atatataagg aagcagagac acaaccctat catcaatact tataaataat gagttgattg
      241 tttgtcaaaa taggcggaat ttcaggattt ccttggcata ccaacgtttg tagcgggttt
      301 ttatatggaa ttttctctta ttcccaccgg gattcgtaat aaatgacaaa tcaaatatat
      361 attatctcaa tgatgtatat gaactacatc actattacac tatctaggag gcaataatat
      421 gaaaaccatc actcaagaac tcaaacaata tataacccac ctattccaac tatctaataa
      481 cgaagcttgg gaatgtgaag cgctggagga agcagcagaa aatattctac cggaacgttt
      541 tattaatgat tctccactgg tacatctcac actcgagact tatacctact ataatgatga
      601 actacatgag ctaagtatct atccatttct aatgtatgcc aataaccaac tcatcagtgt
      661 cggatactta gaccattttg acatggactt tctgtacctt acagatactc aaaatatcat
      721 tattgatgaa cgtcatttac taaaacaagg aggtcaggac catgaataat tggatcatag
      781 ttgctcaatt atccgtcaca gttatcaacg aaatcatcga catcatgaaa gaaaagcaga
      841 agggagggaa atagcatgaa aatcaatcga tacatcacaa gaggaattaa tgaaagtatt
      901 ccactagacc ttcaaatctt actttggcac atggtagaaa aaaaagataa ccagcctcat
      961 acagattacc tacacatttt taaattacaa gaagatgaga atatactctc aatcacacat
     1021 gaacaagaac aacccacata caaattggaa tatcactata caaactatga aaaaaatcaa
     1081 aatgcattac ctaagaaagt ctacgtcatt cgtgaagatg acgtggacgc tttttattat
     1141 gtgatgcttt taccagaaga atactaaagg aagtgacgat gatgaataca atcaaaagta
     1201 cgatacacac agaagccata tttagcgatg atgaacagca ccgctattta ctcaagaaaa
     1261 catgggatga aaagaaagct gcatgcacag tgataacgat gtatcctcat ttagacggtg
     1321 tattatcact cgatctcaca actgttctta tcctcaacca attagccaat tcagaacgat
     1381 acggtgctgt atatcttgta aatctattct ctaatattag aacacccgaa aacctcaaac
     1441 atatcaaaaa tccatacgat gagcacactg atattcattt gatgaaagcg attagtgaaa
     1501 gtgacacagt gattcttgct tatggtgcct atgcgaagcg accagttgtt atcgaccgtg
     1561 tcgaacaagt gatggaaatg ttaaaacctc ataaaaagaa agtaaaaaag ctcatcaatc
     1621 cagtaacaaa tgaaattatg catccactca accctaaggc acgtcaaaaa tggattttga
     1681 aatcatagca aatacatgga ggtcaatatg gaaacaaaac aaatcaacat cgttttactg
     1741 taaatgatat tgttgaaaca attcctgaca atgaattcga cgaattcaga catcaccgtg
     1801 gtgcaacttc gtaccatcct aaaatgatgt taaaagtgat tctatatgcc tacacacaat
     1861 ctgtattctc aggtcgtaaa atagaaaaaa tgcttaatga tagcatccga atgatgtggc
     1921 tatcacaaaa tcaaaaacct tcttataaaa caattaatca atttagagta aatccaaaag
     1981 tagatgcttt attagaatct ttatttattc aattttacag tcagtgtgta aaacaaaatc
     2041 ttatagatga taaagctatt tttattgatg gtacaaaaat tgaagcaaat gccaatcgat
     2101 atacatttgt atggaaaaag agtattcaaa accatgaatc aaagatgaat gaggattcta
     2161 aagccctcta ccatgaattg gtaaccaata aaatcatacc ggaaattaaa gaagatcatg
     2221 ataatgaatt aacaaaagaa gaaatagatt tgattggtag tcacttagat aaagaaatcg
     2281 aagatttaaa ccaacatatc aacaatgaaa aatgtactaa aacaagaaaa caaatacgtc
     2341 tcaaaagaac taaaatcaaa aaatacaaaa agcaaatcaa tgattatttt gagcgaaagt
     2401 atcgatacga atttcaaaaa tctattttaa aggatagaaa tagttattct aagacagatt
     2461 atgatgcgac atttatgaga atgaaagaag atcacatgaa aaatggacaa cttaagccag
     2521 ggtataattt acaaatagca acaaattccc aatttgtttt atcttataat gtgtatcaaa
     2581 atccaacgga tactagaacg atgattccat ttttaaattc aattcaagag acctacggtc
     2641 atttacctga atatattgta gctgatgcag gttatggtag tgaatcaaat tataaggcaa
     2701 ttatagatga ctttaatcga acgccactca taacatatgg aatgtttata aaagataaaa
     2761 ctaaaaaata taaaagtgac atctttaata ctcaaaattg gaactatgac gaaattaatg
     2821 acgaattcat ttgtccgaat aataaacggc taggttttaa aagatatgcc tatcgtcatg
     2881 ataagtatgg ttataagcga gacttcaaat tatatgaatg tgatgattgt tcagaatgtc
     2941 ctctgaaaaa tcaatgtatg aacttcaatt caaaaacaaa caaaaaaata atgaagaatt
     3001 ataactggga atattttaaa tcccaaatta acaaaaagct ttcagaacca gaaacaaaaa
     3061 atatctacag tcaaagaaaa attgatgtgg aacctgtttt tggatttatg aaggctattt
     3121 tgggtttcac tcggatgtct gtccgaggac tcaataaagt caaaagagaa cttggttttg
     3181 tattaatggc acttaatata agaaaagtag tagctcaacg agctgaaaat aatcaaaaaa
     3241 tttataaaaa agacaatttc tatattattt caatagaaat tgtctttttt cacttatcca
     3301 agaactttat gtcccggact cattatgatt tattttttta ttgctgactt gccattaatt
     3361 ctgcttgctc tacaacttgt tccacagcca ttttttgaag atctggtgga tagccatatt
     3421 tctttaatag gcgtctaact tgaactctca ttttagcttt agcactgtct cgtttagacc
     3481 aatcaacact catgttatcc ttaactgttt tagttaactc atgtgcaatg gctcttaact
     3541 ctttatcgcc caaagcttct ttagctgttt catgtgaagc tcgatatact acatacaata
     3601 tacgcaacaa tacgcttgtt tcgattaggt ttaggcattg aatcataaag tgcaatatac
     3661 ttaacacgtt ctttaatatt tgaattaaaa cctagtaaat attgtgctgc cacattattt
     3721 atgtgctgag attttaaaat agagcatttt aatatcgatt caccataacg tatatgttca
     3781 tggcgattca aaatttttaa aacgtttcta tcacatactt tttcacagtc attgtccatc
     3841 attgttttac ttatatatag tgcaggatta aaccagaata tcattttaaa aacaacataa
     3901 agctggttga atattaagtc atgacttttc acatgtgata gttcatgtag aataatatat
     3961 tcaatttctt tgtcattcat ggtttcgact acgacagttg gtagtacaat ttgggatttc
     4021 actaaaccaa ataccatcgg attatcaatg tttgaactat aactaattgt tatatgcttt
     4081 ttgtagaact gcatcttact ttgacatact ttaagtcgtt cattaagata tgacgattcc
     4141 aatgacgaac ttttaataac atcaatttgt cggaatgcct taatcatata aaataagcac
     4201 aacaaactac caaataccca tatcaaaaga atcatatacg ttatatttga ggtctcaaac
     4261 tgattaacat taattgctaa gtctttcgta acagatgatt gttgaccatc taacatatga
     4321 ctaaccgaag aagtcgtgtc agatacattt cgattcatca tatcttttga aaatgtaaaa
     4381 ttcgatattt tgtaaaatgg tattaatgga attaacgtgg agacgagcac taataaccaa
     4441 atcttatgtg acataatatt ttgagtatat tttatataga gcattctcac taaaaaaatt
     4501 acacatatcg tgagcaatga actgattata cttaacatta aaaaagatga taacaccttc
     4561 tacacctcca tatcacaaaa attataacat tattttgaca taaatactac atttgtaata
     4621 tactacaaat gtagtcttat ataaggagta tattgatgaa aaagataaaa attgttccac
     4681 ttattttaat agttgtagtt gtcgggtttg gtatatattt ttatgcttca aaagataaag
     4741 aaattaataa tactattgat gcaattgaag ataaaaattt caaacaagtt tataaagata
     4801 gcagttatat ttctaaaagc gataatggtg aagtagaaat gactgaacgt ccgataaaaa
     4861 tatataatag tttaggcgtt aaagatataa acattcagga tcgtaaaata aaaaaagtat
     4921 ctaaaaataa aaaacgagta gatgctcaat ataaaattaa aacaaactac ggtaacattg
     4981 atcgcaacgt tcaatttaat tttgttaaag aagatggtat gtggaagtta gattgggatc
     5041 atagcgtcat tattccagga atgcagaaag accaaagcat acatattgaa aatttaaaat
     5101 cagaacgtgg taaaatttta gaccgaaaca atgtggaatt ggccaataca ggaacagcat
     5161 atgagatagg catcgttcca aagaatgtat ctaaaaaaga ttataaagca atcgctaaag
     5221 aactaagtat ttctgaagac tatatcaaac aacaaatgga tcaaaattgg gtacaagatg
     5281 ataccttcgt tccacttaaa accgttaaaa aaatggatga atatttaagt gatttcgcaa
     5341 aaaaatttca tcttacaact aatgaaacag aaagtcgtaa ctatcctcta ggaaaagcga
     5401 cttcacatct attaggttat gttggtccca ttaactctga agaattaaaa caaaaagaat
     5461 ataaaggcta taaagatgat gcagttattg gtaaaaaggg actcgaaaaa ctttacgata
     5521 aaaagctcca acatgaagat ggctatcgtg tcacaatcgt tgacgataat agcaatacaa
     5581 tcgcacatac attaatagag aaaaagaaaa aagatggcaa agatattcaa ctaactattg
     5641 atgctaaagt tcaaaagagt atttataaca acatgaaaaa tgattatggc tcaggtactg
     5701 ctatccaccc tcaaacaggt gaattattag cacttgtaag cacaccttca tatgacgtct
     5761 atccatttat gtatggcatg agtaacgaag aatataataa attaaccgaa gataaaaaag
     5821 aacctctgct caacaagttc cagattacaa cttcaccagg ttcaactcaa aaaatattaa
     5881 cagcaatgat tgggttaaat aacaaaacat tagacgataa aacaagttat aaaatcgatg
     5941 gtaaaggttg gcaaaaagat aaatcttggg gtggttacaa cgttacaaga tatgaagtgg
     6001 taaatggtaa tatcgactta aaacaagcaa tagaatcatc agataacatt ttctttgcta
     6061 gagtagcact cgaattaggc agtaagaaat ttgaaaaagg catgaaaaaa ctaggtgttg
     6121 gtgaagatat accaagtgat tatccatttt ataatgctca aatttcaaac aaaaatttag
     6181 ataatgaaat attattagct gattcaggtt acggacaagg tgaaatactg attaacccag
     6241 tacagatcct ttcaatctat agcgcattag aaaataatgg caatattaac gcacctcact
     6301 tattaaaaga cacgaaaaac aaagtttgga agaaaaatat tatttccaaa gaaaatatca
     6361 atctattaac tgatggtatg caacaagtcg taaataaaac acataaagaa gatatttata
     6421 gatcttatgc aaacttaatt ggcaaatccg gtactgcaga actcaaaatg aaacaaggag
     6481 aaactggcag acaaattggg tggtttatat catatgataa agataatcca aacatgatga
     6541 tggctattaa tgttaaagat gtacaagata aaggaatggc tagctacaat gccaaaatct
     6601 caggtaaagt gtatgatgag ctatatgaga acggtaataa aaaatacgat atagatgaat
     6661 aacaaaacag tgaagcaatc cgtaacgatg gttgcttcac tgttttatta tgaattatta
     6721 ataagtgctg ttacttctcc cttaaataca atttcttcat tttcattgta tgttgaaagt
     6781 gacactgtaa cgagtccatt ttcttttttt atggatttct tatttgtaat ttcagcgata
     6841 acgtacaatg tattacctgg gtatacaggt ttaataaatt taacgttatt catttgtgtt
     6901 cctgctacaa cttcttctcc gtatttacct tcttctaccc ataatttaaa tgatattgaa
     6961 agtgtatgca tgccagatgc aatgatacct ttaaatctac tttgttctgc tttttcttta
     7021 tctatatgca tatattgagg atcaaaagtt gttgcaaatt ggataatttc ttcttctgta
     7081 atatgaaggc tttttgtttt gaatgtttct cctactataa aatcatcgta tttcatatat
     7141 gtctctcttt cttattcaaa ttaatttttt agtatgtaac atgttaaagg taagtctacc
     7201 gtcactgaaa cgtaagactc acctctaact ttctattgag acaaatgcac cattttatct
     7261 gcattgtctg taaagatacc atcaactccc caattagcaa gttggtttgc acgtgctggt
     7321 ttgtttacag tccatacgtt caattcataa cccgcttctt ttaccatttt tacttttgct
     7381 ttagtaagtt tggcatcttc agtgtttact attttagcat tacagtaatc taaaagtgtt
     7441 ctccagtctt cacgaaacga agttgtatgg aatataactg ctctgttata ttgtggcatg
     7501 atttcttctg caagtttaac aagcacaaca ttaaagcttg aaatgagcac ttcttgattc
     7561 tgatttaagt ttgttaattg ttcttccact tgcttaacca tacttttaga aagtgctagt
     7621 ccattcggtc cagtaatacc ttttaattct acatttaaat tcatattata ttcatttgct
     7681 atttttacta catcatcgaa agttggcaaa tgttcatctt tgaatttttc accaaaccaa
     7741 gatcctgcag aagcatcttt aatttcatca taattcaatt cagttatttc cccggacata
     7801 tttgtagtcc gttctaaata atcatcatga atgataatca gttgttcatc ttttgtaatt
     7861 gcaacatcta actccaacca gtttatacct tctacttctg aagcagcttt aaatgatgca
     7921 attgtatttt ccggagcttt actaggtaat cctctatgtc catatacagt tagcatatta
     7981 cctctccttg catttttatt tttttaatta acgtaactgt attatcacat taatcgcact
     8041 tttatttcca ttaaaaagag atgaatatca taaataaaga agtcgataga ttcgtattga
     8101 ttatggagtt aatctacgtc tcatctcatt tttaaaaaat catttatgtc ccaagctcca
     8161 ttttgtaatc aagtctagtt tttctgtacc ccttatctgc aattttactt aggattgctt
     8221 ttaacttacc ccttatcagc aattttactg agaactgctt ttaacgcacc tcttatctgc
     8281 aattttgcct agaactgctt ttaacgtacc tcttatctgc aattttactg agaactgctt
     8341 ttaacttacc ccttatcagc aattttgcat ggaattgctt ttaacgtacc tcttatctgc
     8401 aattttactt agaactgctt ttaacaaacc tcttatctgc aattttactt agaactgctt
     8461 ttaacgtacc tcttatctgt aattttactg agaactgctt ttaacaaacc tcttatctgc
     8521 aattttactt agaattgctt ttactattcc tcttattagt ataatctcag taagaatgcg
     8581 tataaaaatg aaaattacaa ccgattttgt aagtgctgac gcctgaggga atagtatgtg
     8641 cgagagacta atggctcgag ccatacccct aggcaagcat gcacgtacaa aatcgtaaga
     8701 taaaaaaata agcatatcac tgtaaacttt aaaaaatcag tttagtgata tgcttattta
     8761 tttcgagtta ggatttatgt cccaagctca tcaagcacaa tcggccacta gtttatttct
     8821 ctatcttata tgttctgata tggtcttcta tactgtataa gtatactttt gaatatggat
     8881 cttgtgtcaa ttcacgttcg aaatcaaatt cttgattatc aaatctgtta aagaatgttt
     8941 cgtattcttc gactgataat tgctctctag attctagcat atttaagtgt ttctctttat
     9001 ctaatgcttt gtcatatcct ttaacgattg aaccactaaa gatttctcct actgctcctg
     9061 aaccataact aaatagacat actttctctt ctggttggaa tgtgtggttc tgtaataacg
     9121 aaattaaact taagtataat gatcctgtat aaatgttacc aacatctcta ttccataata
     9181 cggttctgtt gcaaagttga atttatagta taattttaac aaaaaggagt cttctgtatg
     9241 aactatttca gatataaaca atttaacaag gatgttatca ctgtagccgt tggctactat
     9301 ctaagatata cattgagtta tcgtgatata tctgaaatat taagggaacg tggtgtaaac
     9361 gttcatcatt caacggtcta ccgttgggtt caagaatatg ccccaatttt
//
LOCUS       DAKTLH010000101         9389 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.101, whole genome shotgun sequence.
ACCESSION   DAKTLH010000101 DAKTLH010000000
VERSION     DAKTLH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9389
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.101"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            82..699
                     /locus_tag="O0394_001961"
     CDS             82..699
                     /locus_tag="O0394_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCY0500031.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            827..2293
                     /locus_tag="O0394_001962"
     CDS             827..2293
                     /locus_tag="O0394_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HCY0500032.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGVVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDK
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            2400..3530
                     /locus_tag="O0394_001963"
     CDS             2400..3530
                     /locus_tag="O0394_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0500033.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(3491..3586)
                     /locus_tag="O0394_001964"
     CDS             complement(3491..3586)
                     /locus_tag="O0394_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500034.1"
                     /translation="MKPLVQSFTTSYAVKQQSNYKLSSTPLSSPK"
     gene            complement(3680..5284)
                     /locus_tag="O0394_001965"
     CDS             complement(3680..5284)
                     /locus_tag="O0394_001965"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCY0500035.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTI
                     SATEHTLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAV
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(5271..6431)
                     /locus_tag="O0394_001966"
     CDS             complement(5271..6431)
                     /locus_tag="O0394_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HCY0500036.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINFLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKEHFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(6545..6991)
                     /locus_tag="O0394_001967"
     CDS             complement(6545..6991)
                     /locus_tag="O0394_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCY0500037.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPQI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            7071..7814
                     /locus_tag="O0394_001968"
     CDS             7071..7814
                     /locus_tag="O0394_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCY0500038.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRNYWYKKPNFSRIAQIASYVNPNYALVTRKFVDKAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            complement(8030..8632)
                     /gene="rpsD"
                     /locus_tag="O0394_001969"
     CDS             complement(8030..8632)
                     /gene="rpsD"
                     /locus_tag="O0394_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCY0500039.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(8876..9340)
                     /locus_tag="O0394_001970"
     CDS             complement(8876..9340)
                     /locus_tag="O0394_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCY0500040.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQIVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
BASE COUNT         3051 a         1425 c         1690 g         3223 t
ORIGIN      
        1 attttcagac tatcttgttc tagtaaaaat atagtgataa aatctagttg ttataaacaa
       61 ataagttaag taggtggcac aatgtattca gtgattagta agattttgaa tttcatttta
      121 gttaaaatgt caaaatcatt atacgtaatc ggtaaagata atattcctaa ggatagtaaa
      181 tatgtcgtaa cttgtacgca tgaaagttat aacgaagtca ttatgctagg tatggcatta
      241 tatcctaatc aaattcatta tatggctaaa aaagaattat tcaaaaataa atggattggt
      301 aaatttttaa cgtctttaaa tgcatttcct gtagatcgtg aaaatccagg acctagtact
      361 ttaaaacgcc caatcaattt gttgaaagat aataagacag ttggtatctt cccaacaggt
      421 tctcgcacat cgcaagaagg tgcacctttg aaacgtggtg cttcaacaat tgcaatgtta
      481 agtaagtcac caatattacc tgttgcttat gttggtccta ctaagattca cggtctttta
      541 acaggacaag catacataaa tattggcaaa ccaatcgata ttaacgattt acctaaagat
      601 ttaaaacgaa acgagcgtat cgattatatt acaaaagaga ttgaaacacg aacagcaaag
      661 ttacaacaag aattgcatga aattgtaaaa agtttataaa ataacttgag gtagaaatat
      721 agctggttag taagcctagg tgactataac ggagtatcga agtagttatt ctagttacat
      781 tagtaatctt tatatttcta cattatttta agaaggatga cattcaatgt ataaattttt
      841 ccaaaattta gggcgttcgt taatgttacc agttgccatt ttaccggctg cagcaattat
      901 tgccggaatt ggtaatacgt taaatgcact tcatgcgacg ccgaaaatcg caatgttctt
      961 tactacggta ggtactacga ttttagagca attaggtatt ttatttgcaa ttggtgtagc
     1021 tataggaatg gcaaagaaaa atgatggtgt agtcgcgctt gcagctacgt taggatattt
     1081 tttagttaca gttgtattat caccaatgaa attagcgcct ttattaggaa tgaaagcttc
     1141 ggaaataaat tcagcttttg aaaagatgaa taatggcaat gtctttgttg gtattgtaat
     1201 aggtttaata gcagcttatg catacaataa gtttagtgaa acagaattac cattagcatt
     1261 atcatttttt agtggtaaac gtctagttcc aattatgact gcattttact gtacattttt
     1321 agttgtcata ttgttattct tatggccact actttattca tggattgtaa aatttggtga
     1381 atctatagtc ggtttaggat cgtttggagc tttcatatat ggtgttgcca atagattatt
     1441 aattcctaca ggattacatc atgcactgaa tagtgtgttc tggtttgata cgattggtat
     1501 taatgatatt ggtaaattcc aaagtggcaa agatgctata aaaggtatta caggacgcta
     1561 tcaagcggga ttcttcccaa tcatgatgtt cggtattcca gcggctgcgt tagcaatgta
     1621 tcatacagct aaaactactc agaaaaaaca agtttacggt tggttcctag cgagttcagt
     1681 tgcagcattt ttcgttggtg ttacagagcc gattgagttt gcatttatgt ttgttgcgcc
     1741 aatcttatat gttgttcatg cgttattaac aggattatct ttatttatcg cagctacatt
     1801 ccattggaca gctggatttt catttagtgc aggtttgata gattatgtgt tatcactaat
     1861 taatccagta tcaaatcatc cattgatgtt gcttgttcaa ggtgttgtat ttttcatctt
     1921 atactatgtg attttccgtg tagtaatcca agtatttaac ttgaatacga ttggtagagg
     1981 tgaaaatgaa ttagttgacc caacagttgt aaaagataat attgctcctg gtgaaaatga
     2041 tattaaacaa agtaaatatc atcaacatgc tatacaaata ttagaaggtt taggcggtca
     2101 agagaatatt gttaatttaa ccaattgtgc aacaaggttg cgtctagagt taaaagacac
     2161 atctattatt gataaacaga agattaaaaa cgccggtgca gttggtgtta ctgttaatgg
     2221 caaacattca actcaagtta ttgtcggtac acacgtacag caggttgctg atgaaattga
     2281 aaaacattta taaaatttaa acagctaaag cgcaaatcta acttcaatag ggtttgcgct
     2341 ttttttagtc aaatagtaga atgatttata tctgaatttt cagaaatgga gtggggattg
     2401 tgaagaaaat tttatttgat gttgatggcg tttttttaag tgaagaacgt tgtttcgatg
     2461 tctctgcatt aacggtatat gaattattaa tggataagtg ttatttaggt cttcattcac
     2521 atatagattg ggaaactttg actgataacg atatacaaga cattaggaat cgaatttttc
     2581 aaaaagataa gattttaaac aaattaaaat cgctagggct gaattcaaat tgggatatgt
     2641 tatttatcgt ttttagtatt catttaattg atattttgaa aaagttatca catgatgaaa
     2701 ttgaggcatt catgtatcaa gatgagcctg tcgaattgaa gttgcaaaat ataagcacaa
     2761 acttagcaga ctgttttaat ttaaatgaac aactaccttt acaatttttg gataatgtaa
     2821 aagttggtaa aaataatatt tatgctgctt tggaagagtt tgcaacaaca gagttacatg
     2881 tttcggatgc tactttattt agtttgaagg gtgcattatg gacgttagcg caagaagttt
     2941 atcaagaatg gtatttagga tcgaagttgt atgaagatgt tgaaaagaaa atagcacgaa
     3001 ctacttttaa gacaggttat atttatcaag aaattatttt gagaccagta gatgaagtta
     3061 aggtacttct gaatgattta aaaggtgctg gtttcgaatt aggtattgca acaggtcgtc
     3121 cttatactga gactgttgtg ccatttgaaa atttaggatt gttaccatat tttgaagctg
     3181 attttattgc aacagcaagt gatgttttag aagcagagaa tatgtatccg caagcacgac
     3241 cattaggaaa gccgaatcct tttagttata tcgcagcttt atatggtaat aatcgcgata
     3301 aatatgaatc ttatatcaat aagcaagata acattgtaaa taaagatgac gtatttatag
     3361 taggcgattc gttagctgac ttattaagtg ctcaaaaaat aggtgcaacg tttattggaa
     3421 cattaacagg tttaaaaggt aaggatgctg caggtgagtt agaagcgcat catgccgact
     3481 atgttattaa tcatttaggt gaacttagag gtgtactaga taatttgtaa tttgattgtt
     3541 gtttgacagc ataacttgta gtgaatgatt gaaccaaagg tttcatattg agttacaatg
     3601 aaattaataa tgaaaaaatg ccaagaagca atggaagtaa tccaatgtct tcttggcatt
     3661 ttgaatttac ataaattgtt tatgactgta ccgtcaattc agttgtgaaa atttgattgt
     3721 attcaccaac ttgtttaagt tcatcaatta tattgtttga aacaggttga tcaacggata
     3781 aaatcattag cgcatctccg cccgcttcag ttctacctaa agtcatagat gcaatgttga
     3841 tattgtattt acctaacaat gcgccagttt ttcctaccat acctggagta tcattatgat
     3901 atgacacaat ttgatattga tttggcttta agtctacaga aaaattatta attctaacaa
     3961 ttcttggacc gaaacctgta aagacagaag cgccaacttt aacggaatcg ctatcgtttg
     4021 atagttctac ctctaagtag ttactaaaac ctgtctctgc tttattattt tcaatattta
     4081 atgtcacttg ttgttgattt aacaacatta gagcattgat aatattgact tcaggaccaa
     4141 gatcatcttt taaaatatga gtaataattg tacgtgttaa taaactacta tcaatagagg
     4201 ctaagtcgcc accgtacgta attttaatag agcttggtgc attgtacatt aattgaatag
     4261 ctaattcacc aactgtttgg cttaaattga tgaatgattt tacagtatca tctatattgc
     4321 ttaagtccat tttaggtgca ttcactgcat gcgttacagt accatcaatt aaaatttcga
     4381 tgatttcatt tgaaacagaa attgccactt tttcttgagc ttcgactgtt gaagcaccca
     4441 aatgaggtgt aacaataatt ttatcatgtg caacaagagg cgagtcagtt gcaggttcat
     4501 gttcaaacac atcgatagct gcccgactaa tttgtccttc gtctaatgct tttattagcg
     4561 ccttttcatc aataatacca ccacgtgcca cattgattat ttgcaaacta ggttttgctt
     4621 tggcaaaaaa gacagcatta attaagcctt ttgttttagg tgttagtggt gtatgtaatg
     4681 taacgaaatc agaatgttgg gcaatctcat caactgttgc cttcgtaatg cttaaagatt
     4741 ttgctttttc atccgttaag taagggtcaa aagctagtat tttcattccg aaactttgcg
     4801 cacgtttagc aacacctaaa ccaattctac cagcaccaat gacacctaat gttttatgat
     4861 aaagctcagt acctttaaat gcatttcgat tccattcttt atttgtaagt gattggtgtg
     4921 cttgcggaat atttcgtgcc attgataata acattgccag tgtatgttca gtagctgaaa
     4981 tcgtattacc atctggggca ttaataacta aaataccttt taatgttgca gcatcaatat
     5041 taatattatc tacaccaaca ccggcgcgtg cgattacttt taaagaatca gcagcattta
     5101 tgatattttc agtaaccgta gtttgactac gaacgattaa agcatggtat gaaggtataa
     5161 ttttgattaa tgcttcttcg gacaagccag tttgaatatc tacattgaat tgttcgtgat
     5221 ctaataatgc tttgatacca tcttttgata tgggatctgc aacgagtaca ttaaattgct
     5281 tcatgaataa cctccatata ttttgatata cctttaccga tatagttaac tttacggtgt
     5341 tcagttaaaa taatttctaa agcagatact accgataaaa tatcgaaagg actaattttc
     5401 cccatatgac caattcttaa aatttgacct ttaagatggc cttgaccacc agcaattgtt
     5461 attttaaagc gatttttaag ttcatctttg attattttca cttcatcatt tgtattaggt
     5521 ttgaatgctg taaccgttgg agatgcatct ttatcattga ctaataaagt taaatctaat
     5581 gcttttaagg cgcttcttaa tgcatttcta attgcatagt gtcgtgctat tacgtgattg
     5641 aaaccttctg cttttacggt ttcaacgtat gcatttacac ctctaaataa agacacattt
     5701 ggtgtgaacg gtgtagaatt gtcagcttgt gacgaaatgt atttatttaa gtctagataa
     5761 aattttggcg tagttacttc tttgaaatgt tcttttgcac ggtggctata agctacaaaa
     5821 gctaatcctg gaggtaacat aattgctttt tgactaccag aaacaagtac atcaatttta
     5881 tctttgttaa tgtcaacatc aacagcacca atgcaactta cgccgtcaac tacaaaataa
     5941 atattactat taaattgatt aatggcattt cctaactcgt gaatagggtg tagcactgtc
     6001 gtagatgttt cgcaatattg actaaatact gctttaactt caacatttaa agttgaaagg
     6061 aaattgatga aatctttgac atctacagct tctccccatg ttacgtcata aatatgcaca
     6121 tttttgtaat aagtttgtgc aatttgttta aatcggttac caaaggcacc tgaaacaatg
     6181 acaacgaagt gatcttcagg gtttacaatg ttcaacatac tagcctccaa gacgcttgta
     6241 ccgctagatg ttaaaataag tacatcattt tgactcccaa atattggctt tagaccttga
     6301 aatgcttgtt gtgcgatgtc ttcaaaatct ttagaacgat gaccaaccat aggtgcttga
     6361 atttctctca taatggcatc aggtacaggg gttgggccag gtgttaataa caacggttga
     6421 tgataataca tttatatgcc tcctataaat aagatttcac tattttagca aattttctga
     6481 aaatttaaaa gcctaaaaaa gtgataacgc tatattgtcg aacaaacgtt atcacttaac
     6541 gattttattt ggcctgaatg atgggataga tttttatgtc cacattattt cttacagcat
     6601 ttgaaatcat gcaattatta tctgcaattg ttatcaattt tggtaatcgc ttttctaatt
     6661 gtgctatttg atcacttgga atttgaattt gaggatggtg cacaattttt gacatactga
     6721 attttccgtt atttaaacaa gctgttccaa tcgattgttg ttcaattgaa atatctgtga
     6781 actttgcacg ttcaagagta gctgctaatg agatgatata acatgatgaa gcggctgata
     6841 ctaacatttc atcgggattt gttcctatac caacaccacc taaagaagca ggtatagaaa
     6901 tattctctga aagtatgtcg ccttgaacgt ttccgacatt gttacgacca ccttgccaag
     6961 aagtttggac tttaaagtca tgttgatgca attgctttaa cctccaatat aattgtgata
     7021 gtttaatttt agaatagttt atcaaagttt aacagaaagg tgacttatca atgactctga
     7081 ataaactgaa agatgaatta caaattgttt cgcaccgtgg attgccgagt gattttcctg
     7141 aaaatacaat ggtcggttat cgagaggtaa tggggctcaa tgttgctatg ttagaaatag
     7201 atgttcattt gaccaaagac caacattttg ttgtgataca tgatgaaaca attgatagaa
     7261 catcggatgg taaggggcgt attgctgatt acacattatc gcaattaaaa tcatttgatt
     7321 ttggtagtta taaagatgtt gcttttaagg gagaacgaat acccacgtta gatgaagtac
     7381 tttcattatg cttaaagtat gataaaaagc tattgattga attgaaaagt ccgaatttat
     7441 atccagaaat agaatgtaaa ttgctggcat ttttggaaga aaagaaagtc gatgcgacac
     7501 aagtagttat acaatctttt gatatcgaat gcatcgaaaa gttaaacaca ttgggcagta
     7561 tatatgaatt aggtgtgctt tgtagtaaac gtaattattg gtataaaaag ccaaactttt
     7621 caagaattgc tcaaattgct agttatgtta atcctaatta tgcattagtg accagaaaat
     7681 ttgttgataa agcacatcat catcaattac aggttatgcc atacacagtt aataaattga
     7741 aaactggaga aaaattaaga caaatgggtg tagatggatt aattacagat aatccgaaat
     7801 tatttattaa gtgagacgat gaatggtaat ttgatagagg gatgaggatt cgtatatcgc
     7861 aattgataat tttaaaaact aatgatgaaa tactaaagat aattaaacaa ataagacttg
     7921 taaattctaa gttgattgta aaatagggta gctccatata attttgaatc tatcattttt
     7981 aaattaataa aaaaaacacc cacaattgtg ggtgtttgta ttgaacgtat tatcttgagt
     8041 agtactcaac gattaattgt tcgttaattt cagcaggtaa ttcgctacgt tctggtaaac
     8101 gtacgaaagt accagttaag ctgtcagcat caaagtttaa gtactcaggt acgaaattgt
     8161 tgatttcaac tgattcaacg atgatgttta atttttgaga tttttcacga actgaaattg
     8221 tttgaccagg tttaacagaa taagatggaa tatcaacacg tttaccatct actaagatat
     8281 gaccgtggtt aactaattga cgtgcttgac gacgagtacg agctaaacct aatgaataaa
     8341 caacagcgtc taaacgactt gctaataaga tcatgaagtt ttcaccgtgt acaccgaatt
     8401 ttttaccagc gatgtcaaat gtgttacgga attgtctttc agtcattcca tataagtaac
     8461 gtaatttttg tttttcacgt aattgtaaac catattctga taatttttta cgttggtttg
     8521 gaccatgttg tcctggtgcg taaggacgtt tttctaattc tttaccagta ccgcttaaag
     8581 agatacctaa acgacgagat tttttccagt ttgaacctct gaatcgagcc ataataagac
     8641 tcctccttta attctttttt gttgttatga ataaacaaaa aagagtgttg catgcttact
     8701 aagatatatt gttttatgtg tcttcacctt atagcgacag ttacgtgaca cgtccgcatt
     8761 gggaacaaca tagcgctcaa taatatacaa ctgctacttt cgttaattca cacaaagatt
     8821 attgtaacat tttcctgcta actgtcaaca ctttattgaa aatgctgatg tccttttatg
     8881 cgagttgctt ttcaataatt ttaacaattg cttcaagatg ttctttgtca ttgtcatcaa
     8941 atcgatccgt tataggggca tcgatatcta agacgccgat aattttatca tctttgaaaa
     9001 ttggaacgac aatctcagac ttactattag catcacaagc gatatgtcct tcgaattgat
     9061 gaacatcagc tacaatttgt gtacgacgtt ctgaaacggc tgtaccacat acaccttttc
     9121 caattggaat gtggacacaa gcggggtgtc cttggaaagg tccaagtata agttcatttt
     9181 gttccaataa gtaaaagcca acccaattaa tttgatctag attatcattt aataaggcag
     9241 acatgttact taaaatagca atcatgtgat gttcatcttc aataaggctt gctgcttgtt
     9301 tctttaataa tgtgtagttt gttgggttaa ttgttgtcat atttaaaacc tcttattttc
     9361 atagatttta taatagtata gaatttcga
//
LOCUS       DAKTLH010000102         9379 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.102, whole genome shotgun sequence.
ACCESSION   DAKTLH010000102 DAKTLH010000000
VERSION     DAKTLH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9379
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.102"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            239..1465
                     /locus_tag="O0394_001971"
     CDS             239..1465
                     /locus_tag="O0394_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0500041.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAEISGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDAIFVVDSNNHLLGFLDIEDINQGIRGHKSLRDTMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDQQRLVGLITRANVVDIVYDTIWGDSEDTVQTEHVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            1462..2097
                     /locus_tag="O0394_001972"
     CDS             1462..2097
                     /locus_tag="O0394_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0500042.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIVLTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            2114..3055
                     /locus_tag="O0394_001973"
     CDS             2114..3055
                     /locus_tag="O0394_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0500043.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKNPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            3055..3750
                     /locus_tag="O0394_001974"
     CDS             3055..3750
                     /locus_tag="O0394_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0500044.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            4044..5444
                     /locus_tag="O0394_001975"
     CDS             4044..5444
                     /locus_tag="O0394_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0500045.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLITVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEVWYIISSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            5670..6605
                     /locus_tag="O0394_001976"
     CDS             5670..6605
                     /locus_tag="O0394_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCY0500046.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            6927..7778
                     /locus_tag="O0394_001977"
     CDS             6927..7778
                     /locus_tag="O0394_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCY0500047.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYQTWK
                     DGMNPIK"
     gene            complement(7870..9366)
                     /locus_tag="O0394_001978"
     CDS             complement(7870..9366)
                     /locus_tag="O0394_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCY0500048.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDAVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDS
                     VTSKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQTNYKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASMLNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLSLCICIALALILYALPY
                     LILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT"
BASE COUNT         3073 a         1301 c         1885 g         3120 t
ORIGIN      
        1 ttttaaatgt cgaatatacg aatgtgcaaa caaagtaatc ggtagaaatt caacatacat
       61 agcgccgttt actgttaagt attcacatta cagatgaaaa atataaaatt ctacataatc
      121 aagaccatga tgtgtacttg tttaacttat gactctattt gtttaacaat tgcgataatg
      181 gtctttttat tttatgcgta tcattcgtca tattttttat gaggaaggag aaatgattat
      241 gttaagtatt aagcatttaa cgaaaattta ttctggtaat aaaaaggcag tagatgacat
      301 ctctttagat attcaatctg gggaatttat cgcatttatt ggaaccagtg gaagtggcaa
      361 aacgactgct ttaagaatga taaaccgtat gattgaagcg acagaaggac aaattgaaat
      421 tgatggtaaa gatgttcgga gtatgaatcc tgtcgaattg cgtagaaata ttggctatgt
      481 tattcaacaa attggcttaa tgcctcatat gacgattaaa gagaatattg tgttggtacc
      541 caaattgttg aaatggacta aagaggaaaa ggataaacgt gcaaaggaat taattaaact
      601 tgtggattta ccggagtcat ttttagagcg ttatccagca gaaatatcag gtgggcaaca
      661 acaacgtatc ggtgttgtaa gagcacttgc ggccgaacaa gatattattt taatggatga
      721 accttttggt gcattggatc ctattacgag agatacgtta caagatttag ttaaaacgtt
      781 acaacgaaaa ttaggcaaga cgtttatctt tgtaacacat gatatggatg aagcgattaa
      841 attagcagac aaaatttgta ttatgtcaga aggtaaggtg gtgcaatttg atacgccaga
      901 caatatttta agacatcccg caaatgattt tgtacgtgat tttataggac aaaatagact
      961 gattcaagac cgtcccaatg acaagactgt agaaggtgta atgattaaac caatcacgat
     1021 acaagcagaa gcaacactga atgacgccgt tcatattatg agacaaaaac gtgttgatgc
     1081 tatttttgta gtagatagta ataaccattt actaggtttc ttagacattg aagatataaa
     1141 tcagggtata cgtggacaca aaagtttacg agacaccatg caacaacata tttataccgt
     1201 tcaaattgat tctaaattac aagattctgt acgtacgatt ttaaaaagaa acgttaggaa
     1261 tgtacctgtc gtagatgatc aacagcgttt agtaggactg attacgcgtg ccaatgttgt
     1321 tgatattgta tatgacacga tttggggcga tagtgaggat acagtgcaaa cagaacatgt
     1381 gggggaagac actgcgtcct caaaagtgca tgagcaacac actactaatg tcaaagtacg
     1441 tgacatagga gatgataaat catgattgag ttcctacatg aacatggtgg acagttgatg
     1501 tcgaaaacac tggaacattt ctatatttct atagtggcat tattacttgc catcattgtt
     1561 gcagtaccta taggcatttt attatcaaaa acaaagcgaa ctgccaatat tgtattaact
     1621 gtggcaggtg tcttacaaac tattccaaca ctagctgtac ttgctattat gataccgatt
     1681 tttggtgttg gtaaaacgcc tgcaattgta gcgctattta tttatgtatt attacctatt
     1741 ttaaataaca cggtactcgg tgttcaaaat attgatagca acattaaaga agctggaaaa
     1801 agtatgggaa tgacacaatt tcaattgatg aaggatgttg aattgccgtt agcattgccg
     1861 cttatcattg gtggcattcg tttgtcatct gtgtatgtaa ttagttgggc tacacttgca
     1921 agttatgtag gtgcgggtgg attaggtgat ttcattttca atggtttaaa tttatatgat
     1981 ccactgatga ttgtaactgc aacggtactc gttactgcac tagcattagg tgttgatgcc
     2041 ttattagctt tagttgaaaa atgggtagtt cccaaaggct taaaagtatc tggataatta
     2101 ggaggctaag ataatgaaga aaattaaata tatacttgtc gtgtttgtct tatcgcttac
     2161 cgtattatct ggatgtagtt tgcccggact aggtagtaag agcacgaaaa atgatgtcaa
     2221 aattacagca ttatcaacaa gcgaatcgca aattatttca catatgttac ggttgttaat
     2281 agagcatgat acacacggta agataaagcc aacattagta aataatttag ggtcaagtac
     2341 gattcaacat aatgccttaa ttaatgggga tgctaatata tcaggtgtta gatataatgg
     2401 cacagattta acgggagctt tgaaggaagc accaattaaa aatcctaaga aagcaatgat
     2461 agcaacacaa caaggattta aaaagaaatt tgatcaaacg ttttttgatt cgtatggttt
     2521 tgcgaatacg tatgcattca tggtaacgaa ggaaaccgct aaaaaatatc atttagagac
     2581 agtttcagat ttagcaaagc atagtaaaga tttacgttta ggtatggata gttcatggat
     2641 gaatcgtaaa ggcgatggct atgaaggatt taaaaaagag tatggttttg actttggtac
     2701 agtgagacca atgcaaatag gtctagtcta cgacgcatta aactcagaga agttagacgt
     2761 tgcattaggt tattctacag atggtcgaat tgcggcgtat gatttgaaag tacttaaaga
     2821 tgataaacaa tttttcccac cttatgctgc gagtgctgtt gcaacaaatg aattattacg
     2881 gcaacaccca gaacttaaaa cgacgattaa taagttgaca ggaaagattt cgacttcaga
     2941 gatgcaacgc ttgaattatg aagcggatgg taaaggtaaa gaacctgctg tcgtcgcaga
     3001 agaatttttg aaaaaacacc actattttga taaacagaaa ggtggtcata agtaatggaa
     3061 ggtaatttat tacagcaatt attcaattat tatgttacga actttggtta tctatgggat
     3121 ttatttttca aacacttatt aatgtctgtc tatggtgtgc tgtttgcagc tttaattggt
     3181 attccattgg gaatcttgct tgcaagatac acaaaacttt ctggatttgt aattacaatt
     3241 gcaaatataa ttcaaacagt tccagtcatt gcaatgttag ctattttaat gttagtcatg
     3301 ggcttaggtt cagaaacagt agttttaaca gtgtttttat atgcgttact tccaattata
     3361 aaaaacactt atactggtat agctagtgtt gatgcgaata ttaaggatgc tggcaaaggt
     3421 atgggaatga cacgcaatca agtgctacga atgattgaat taccgttatc tgtttcggtt
     3481 attatcggtg gcattcgtat tgccttggtt gttgcgatag gtgttgttgc cgttggatca
     3541 tttataggag cacctacgct tggtgacatt gtgattcgtg gtacaaatgc gacggatggc
     3601 acaacgttta ttttagcagg tgcgattccg attgctatca ttgcaatcgt cattgatgta
     3661 ctattaagat ttttagaaaa acgattagac ccaacaacac gacatcgtaa aaatcaatct
     3721 aatcatcggc cgcaaagtat taatatgtaa tagtagaaga tgtttataat ttagcgattt
     3781 cgtttcatga tttataaaaa atgaggctac tcaaggagct caaataatct ttgagtagcc
     3841 tttttatagg ttgtgtttgt atgcgtttac actaaaatag caattattat catgaaagtt
     3901 tttggataaa aagcgttaat tattgtaaaa atactaaaaa atgagatgtt ttatttatat
     3961 ttttctgcaa atttatgata ttgtttctta atatatcata ttaaaaattt gtttttctta
     4021 aacataggag gcttatctaa ttcatggaca catcaaaaca atttagaggt gacaaccgat
     4081 tgcttttggg tatcgtttta ggggttatta ccttttggct attcgcgcag tcacttgtta
     4141 atcttgttgt cccattacaa tcaacatata gtagtgacgt tggaacgata aatatcgctg
     4201 ttagcttatc tgccttattt gctggtttgt ttatcgtagg tgctggtgat gttgctgata
     4261 aatttggtcg cgtcaaaatt acttatgtag gattgatatt aaatgttgta ggttcattac
     4321 tcatcatcat tacacctttg ccagcatttt taattatagg tagaataatt caaggtttgt
     4381 ctgcagcatg tattatgcca tcaacacttg ctattattaa cgaatattat attggtacaa
     4441 gaagacaacg tgccttaagc tattggtcta ttggttcttg gggtggtagt ggtatttgta
     4501 cgttgtttgg tggcttaatg gctacatata taggttggcg ttcaatattt gttgtttcaa
     4561 ttctattaac attattagca atgtacttaa tcaaacatgc acctgagact aaagcagaac
     4621 caatcaaagg tatgaaagca gaagctaaaa agtttgacgt tattggttta gtcattttag
     4681 tagtgacgat gttaagttta aatgtaatca tcacacagac gtctcatttt ggtttagttt
     4741 caccgttaat tctaggttta attgttgtgt ttatctgttc attagttggt tttgtatatt
     4801 acgaaaataa aataaagcat ccacttgttg atttttcaat ttttaaaaat agaggataca
     4861 gtggtgcaac tatttcaaac ttcttattaa atggtgtagc aggtggtgca cttatcgtta
     4921 ttaacacgta ttatcaacaa caattaggat ttaattcttc gcaaacgggt tatatttcat
     4981 taacgtattt aataacagtg ttgtcaatga ttcgtgtagg tgaaaagatt ttatctcaac
     5041 atggtccgaa gcgcccacta ttactaggaa gtggctttac agtgattggg ttaatcttat
     5101 tgtcgttaac atttttacca gaagtgtggt atatcatatc tagtatagtt ggatatttat
     5161 tgtttggtac tggtttagga ttatatgcta caccatcaac tgatacagca gttgctagtg
     5221 cgccagatga taagtcgggt gttgcttcag gtgtgtataa aatggcgtca tcattaggaa
     5281 atgcatttgg agtagcagta tctggtacgg tttatactgt gttagcagct aatttaaatt
     5341 tgaacttagg tggtttcaca ggtatgatgt ttaatgcctt gctagcaatt gttgcatttt
     5401 tagtcatttt actattagtt cctaaaaatc aaacgaattt gtaaaactga aatgaaagca
     5461 agttattatg tagggatttt aaaggaaatt ttgtgaaagt aagtttatca tacacactta
     5521 atgttgcgta ttgacgttta atgttaggtg tgttctttta tagacgataa aagctgtgtg
     5581 catattaagc gaatgatttt caaattgacg ctaatatgcg aaagtagtat ttttaaaatg
     5641 aacaacaacg atgaagaggg gtttatagga tgaaaattgc aattgctgga tcgggtgcat
     5701 taggtagtgg ctttggtgcc aaactatttc aagcaggata tgatgtcaca cttattgacg
     5761 gatatacatc tcatgttgaa gcggttaagc aacatggatt aaatataacg attaatggag
     5821 aggcattcga gttaaacatt ccgatgtatc attttaatga tcaaccggac gaaagcattt
     5881 acgatgttgt ctttctattt ccaaagtcta tgcaattaaa agaagtgatg gaagatatga
     5941 agccacatat tgataatgaa acgatcgtcg tatgtacgat gaatggtctg aagcatgaag
     6001 aagtcattgc gcagtatgtt gctcaatcac aaattgtcag aggtgttacg acttggacgg
     6061 caggtcttga aagccctgga cacagtcatt tacttggtag tggaccagtt gaaataggtg
     6121 aactagtgga tgaaggtaaa gaaaatgtta taaaagttgc tgatttactt aacgaagcgg
     6181 aattgaatgg tgtcattagt aaagatttat accaatcgat ttggaaaaag atttgtgtta
     6241 atggtacggc aaatgcatta agcacagtgt tggagtgtaa tatggcatcg ctgaatgaaa
     6301 gtagttatgc gaagtgtttg atttataaat taacgcaaga aatagtgcat gtagcgacga
     6361 ttgataatgt tcatttaaat gttgatgaag tatttgaata tttagttgat ttaaatgaaa
     6421 aagttggtgc gcattatcca tccatgtatc aagatttaat tgttaataat agaaaaactg
     6481 aaattgatta tattaatggc gcagttgcaa cattaggtaa acaacgtcat attgaagcgc
     6541 cagtcaatcg ctttattact gatttaattc atactaaaga aagtcagcga cacgcacagg
     6601 attaattggt gttttaggta tgattacaag gaaatgacaa tcacgtgata ttacggtcat
     6661 tattaagatt gaaatgtaat aaataaagaa cagcagtaag gtactttcaa attgaaatga
     6721 tcttggtgct gtttttcttg attgatcttc gtcataattc agatttgtca taggtctacg
     6781 acatactatt agtatttact agacagtttt tacgacgaca ctttgaaaaa ttttgaggca
     6841 aatcatttgg aagtctcacg tgaattttgt aaactcatca agcaagtaat tatattaaaa
     6901 agacaaatag agaaaaggtg tttataatga gtaaaatttt tgtaactggt gcaacgggcc
     6961 ttattggcat taaattagtt caaagactaa aagaagaggg gcatgaggtt gctggtttta
     7021 ctacatctga gaatggtcaa caaaagctag ctgctgttaa tgtaaaagca tatattggtg
     7081 atatattaaa agctgatact attgatcaag cgttagcaga ttttaaacca gaaatcatta
     7141 tcaatcaaat tacggattta aaaaatgttg atatggcagc aaatacgaaa gtacgtattg
     7201 aaggttctaa aaacctaatt gatgcggcga aaaagcatga cgttaagaaa gtaattgccc
     7261 aaagtattgc ctttatgtat gaacctggcg aaggattagc aaatgaggaa acttcacttg
     7321 attttaactc aactggcgat agaaaagtaa cggttgatgg tgtggttggt ttagaagaag
     7381 aaacggctcg tatggatgaa tacgttgttt tacgttttgg ctggttatat ggcccaggta
     7441 cttggtacgg aaaagatggc atgatttata atcaatttat ggatggtcaa gtgacacttt
     7501 cagatggcgt aacatcattt gtgcatcttg atgatgcagt tgaaacatct attcaagcta
     7561 ttcattttga aaatggtatc tataatgtag cagatgatgc acctgttaaa ggttctgaat
     7621 ttgcagaatg gtataaagaa caacttggtg ttgaaccaaa tattgatatt caacctgcgc
     7681 aaccatttga acgtggcgta agcaatgaga agtttaaagc gcaaggtggt actctgattt
     7741 atcaaacttg gaaagatggc atgaatccaa ttaaataata atttatccgt ttaatataca
     7801 aagaataaag acttggtcga atcgtggatg atatattatc aaacgcacgg ctcgaacaag
     7861 tcttttttat tatgtcttcg ttatctttgt atgaaggaat aacagaatta caattaatgt
     7921 actgaataat gcaattaatg ttgtgattag tgctaattta atttctattg gtagccaagt
     7981 cagtacaaaa gaccaattat tgctaccgag aatgagatat ggtaatgcat ataatatgag
     8041 cgctaaagcg atacatatac ataatgataa ccaactcaat acagcaatcc gttttgatct
     8101 acgcaaaaga atttgaccat ggcgcatttg ccaagcacga taagctgaga atataaatgc
     8161 taataaaata agtgttgtca tcaaaacggt aacaatatta aattgatctt tatattgatt
     8221 gagcatagac gcaaccgtcg aatatcgctt gtgatttaca atttgtgtat taagatgctc
     8281 aactaatttg ggtacatatt ccgaatttag atttgcaagt acaacaattc cataattttg
     8341 ttttggattt agtaaaataa atgatgaaaa gttatctagc gttcctgaat gaaacactaa
     8401 atgttcatca ttattggtaa accagccgga agcatatgca ttggcattag gttcaccaat
     8461 tgttgaagat aaatttttat gtgattgttg aactaatgat ttgtatttat caggtggatt
     8521 aagttggaat tttatccaat gttccaaatc ttcagttgat gtcatcatat atgctgatgg
     8581 tgtatcccaa aggttaaatt caggtttaga gacgacaggt gtcgaacctt gtaattcata
     8641 gccaatagca tcatgttttg atttgtaatt ggtttgtttg aatgatgtat gtgtcatatg
     8701 caaaggcttg agccatgaat ttgtaatata ttttgtatag gattgcttcg taacgttttg
     8761 gataattaaa cctaataaat catagttcat atttgagtat tcaaattctt ctccgggctt
     8821 atgatgtaat tcatcaccca taattgcacg ggttacatca tttaaacgat tatttttgct
     8881 tgtcacagaa tcttcgcttg taatatcact aggtatacca cttgtttgag ccaaaagttg
     8941 cttaatcgta atagtttcat tttgaccatt atagttcatt ttaaaatgag gcacatgttt
     9001 ggatacggca tcatttaagt ttaatcgacc ttcttgagct aattttaaaa ttgcaagacc
     9061 tgtgaaagct ttcgtattag aagcaatttc atactttgtt gtgggagaag ctttgacttt
     9121 tttatcaaca tcttgataac cataaccttt atttaaaaaa actttgccat tttttactat
     9181 taaaattgat gctccaggaa tgtgtccctt ttgtaaatca tgctcgataa ttgtatctat
     9241 ttgttgttgc gaatcattgg ttaaccgtgt cttcgtattg ctatttaatg ttatatatat
     9301 agcaattgaa atggcgacta aaatgataat agaaatggat agaaaataca gttttttagt
     9361 agtcataaac gttcctttt
//
LOCUS       DAKTLH010000103         9229 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.103, whole genome shotgun sequence.
ACCESSION   DAKTLH010000103 DAKTLH010000000
VERSION     DAKTLH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9229
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.103"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..1030)
                     /locus_tag="O0394_001979"
     CDS             complement(<1..1030)
                     /locus_tag="O0394_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCY0500049.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEA"
     gene            1117..1428
                     /locus_tag="O0394_001980"
     CDS             1117..1428
                     /locus_tag="O0394_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCY0500050.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            complement(1553..2395)
                     /locus_tag="O0394_001981"
     CDS             complement(1553..2395)
                     /locus_tag="O0394_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0500051.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVLGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDNGENIDAYILRETL
                     VDNN"
     gene            complement(2865..3509)
                     /gene="trmB"
                     /locus_tag="O0394_001982"
     CDS             complement(2865..3509)
                     /gene="trmB"
                     /locus_tag="O0394_001982"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCY0500052.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
     gene            complement(3524..4315)
                     /locus_tag="O0394_001983"
     CDS             complement(3524..4315)
                     /locus_tag="O0394_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCY0500053.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
     gene            complement(4865..5713)
                     /gene="dat"
                     /locus_tag="O0394_001984"
     CDS             complement(4865..5713)
                     /gene="dat"
                     /locus_tag="O0394_001984"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HCY0500054.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLKNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKY
                     IESHSI"
     gene            complement(5717..7126)
                     /gene="sapep"
                     /locus_tag="O0394_001985"
     CDS             complement(5717..7126)
                     /gene="sapep"
                     /locus_tag="O0394_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HCY0500055.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            complement(7876..8298)
                     /locus_tag="O0394_001986"
     CDS             complement(7876..8298)
                     /locus_tag="O0394_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCY0500056.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFKEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
     gene            complement(8315..9010)
                     /locus_tag="O0394_001987"
     CDS             complement(8315..9010)
                     /locus_tag="O0394_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCY0500057.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKITVRGELIEYIENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            complement(9007..>9229)
                     /locus_tag="O0394_001988"
     CDS             complement(9007..>9229)
                     /locus_tag="O0394_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCY0500058.1"
                     /translation="FLYSFIMMLGVELVFFLANLFLEPTKLGYLIIIILGVTVGILIY
                     GTITIKTRLADEFLGEIPEKLRRRVRFLR"
BASE COUNT         2912 a         1748 c         1237 g         3332 t
ORIGIN      
        1 tggcttctga aagtaaagat ctagctgcaa aatagtctct tatatcaaat actgagtctg
       61 tactatgaat atatcgtgca catacaccaa taactgcagt cggaatacca atattagctt
      121 tatgaatttc tccaccatct gttccacctg gtgacatata gtattgatgt tcaatgtcat
      181 gtgcttctac taactttaat aaatagtctc taaatacagg ctttaaaatc attgtaccgt
      241 cttttatgcg aattaacgtc cctttaccaa gttcaccaga taatggttgg tttcctttaa
      301 cgtcattggc aggtgaacaa tcaactacaa atgcaacgtc tgggtctatc atctctgcag
      361 atgctttcgc acctcgtaat ccaacctctt cttgaacatt tgcgccaaca tacaagtcta
      421 catctaattc tatatctttt aataattcta gtatttcaat tgccaagaca caaccataac
      481 gattatccca tgctttagca ctatatcgat gttcagataa ctgtgtgaat ggcgtgtgag
      541 gtacaattgt atctcctata tctattccgc gctcacgcac ctcatcttca ttttgagcac
      601 ctatatctaa tgttaaatct ttaatttccg gtgcaccttc actaccagta cgaaaatgtt
      661 taggtatatt agaaacaaca ccgataattt tatcgccatt tctattttta attactaagc
      721 gttgtccttg ccaaatatca tttgcaacac cacctaaatt tgtgaattga atcattccat
      781 ttttagtgat atttgtaatc ataaatccga tttcatccat atgtgctgca atcattacac
      841 gttttgcatt tggattttta gattttttca caccaaaaaa tccacccata cgattttcaa
      901 taaattcatc tacgtacggc gccatttgct gagtcatata attttttact tcttcttcaa
      961 aacctggtgc cccatgaagc tcagttaaag tttgaattcg ttgtaatgtt acttttttat
     1021 ttatgttcat aaaagttctc actccttata agttattata tcatcttcaa tatggtaaac
     1081 taatgatatg aatttgaaat caagggagca taaatgatga ctaaactgaa atatataatt
     1141 ccaacaataa ttgcagtagc cattgtaatt atttctacca tttcaatcat ccaatttatt
     1201 aatcgtaaac gctataatcc cgttaaagta cttaatgaag taaaatcata ttttatgaat
     1261 gtcaaaggct catatatcgt ttatgaacca ttcgttcatc ctgaaactga taaataccgt
     1321 ttagtttatc aaggtggaat tacaactatt aaaaatggtc aaaatattca ttatgatttt
     1381 tatgcagatg catatactgg tgaagtcatt aacattgtag agcgttaata cgaattttat
     1441 ccatatttga tttggatcgt aaaacttaaa aatgagccac accctaatta gtaagattgg
     1501 atgtggctca attttttatt tttgaaaatt tgtttagtaa tacatcttta ttttagttat
     1561 tatcaactaa tgtttcacgt aaaatatatg catctatgtt ttcaccatta tcgctgtatt
     1621 ttacagcaaa gtagttttca tcatgataaa acaagaacca atattgttgc tgaataaaat
     1681 atggtatcat gcgttctttt tcacgaatcg attgcatagg ataatcatca tatgccgtta
     1741 cccatagagg atttttatgt gcagtagttg ggaatatatc acccatatga actgctttat
     1801 ctccttgact ttcaatcgta ataatcgtgt ggccaaagct atgacctcca ctatgttgca
     1861 tcttgatacc cagaaccggt tcaaaatgtt tttcgaataa aatcaacttg ttactataat
     1921 cgcctttatt cttatcccag taagttgatt tacttcttat attaggtgca ataaactcat
     1981 gccactcatc ttgttgcaca acatgaatcg cattttcaaa aattgcatgt cctgcttgat
     2041 cagtcaaacc ggcagcatga tcaaaatgca tatgtgtcat tagcacataa tcaatatcct
     2101 ttggcgttaa attataattt gccaaatcag caattatatg actttcttca tctactccaa
     2161 aattacgtaa ttgcttttca gataatttac cattaccaat acctgcatct ataatcaaat
     2221 tatattgagc cgtttgaatc aaaattggat gtgtcggtaa attgatttga tttcgttcat
     2281 ttgcattgta ttgctttgac cacaacggct tcggaacaac accaaacatt gcaccgccat
     2341 ccatttttgt attgccacca tttagataat gaatagatat atccccgatt ttcataacat
     2401 cacctattct ttctgtattc gttattacaa ttagcatgtg tttattacat catatcacta
     2461 ttcataaatg cgaacaaaaa cgaaacgccc cacactaata cacttctaac tacttataaa
     2521 gattaatgca atcatattat atgtaaatgc atcataacca tcggcctgta tttttaaatt
     2581 aataattaaa atttacaagt aaaatgcaag ctttattctt ttaaatcaaa ggtatttagt
     2641 ataacattgt tcataggcgc attatctcct ttagttattt gtttagtcac ttttaattat
     2701 agagataatt gcgctttttt tatattcaaa atcaaaaaat cggacagatg aaaagttaaa
     2761 aactttttcc atcagtccga tttattatag aacctttatc tatagaacca ttatagtacg
     2821 aaaaaacact tatgattgta tgtgtacata ccctcataag tgctctattt ttgtgaatgg
     2881 aatttcgctt ccatacgata aatacgtgac cctttatccg aaaatttctt ttcatattct
     2941 gttaaaatat tactgccatc gtcttcttga tgtaaattta gatttatttt tgtaaaatac
     3001 attccaaatt gagacatact ttctaaactg taggcaaata gtcctctgtt atcagtttta
     3061 aaatgtaaat ctccttcatc atttaagatt tgttgataca acgctaaaaa cgtatgatac
     3121 gttaaacgtc gttttgcatg acgatttttt ggccatggat ctgaaaagtt caaataaata
     3181 cgcgaaactt cgccgtcttt aaaatattca tttaattcaa tggcgtcatt acaaataatc
     3241 tttaaatttg ttaaacccat ctctttaact ttatccaata ctttataaac gatacttttc
     3301 tcacgttcca ttgaaatata gttaatatga ggattttgag cagctaatgt tgtaataaac
     3361 tgccccatac ccgaaccaat ttcaatgtgt atcggttgcg ttttatcaaa ccattcagtc
     3421 attttccctg catgttgacc gtccatgtca accaattcag gatgatcttt taaataatct
     3481 tcagcccatg gtttgtatcg aactctcata ttttattctc ctcttaaata aacatgttac
     3541 tattcataac ttcatttagg aatttaagcc aagtgttcat atccttatat cttttttgct
     3601 cttcatacca ttgaacaaga cctatagatt gaattaccgt ataccatttc atacgtttat
     3661 ttaaattcaa gctctcttga acaccatatg tttcaagcca ttcagaccat tgttgttgtg
     3721 gaacatagtt gtaaagcagc attccgatat caattgccgg gtctgcaatc attgcacctt
     3781 cccaatcaac taaaaatagt tcatctcgat cggataataa ccaattatta tgattcacat
     3841 caccatgtac aacagtgaaa aaacgcgaat ctaaactcgg tatatgctct tctaaatagg
     3901 ttaatgattt tctcacaata tgatgtgtta aaacttctct tgataaagag gcattaattt
     3961 tattaagcat aatctcagga gtaataggtt ccatttccat acgctttaac atacttaata
     4021 aaggtctaga attgtgtatc ttctttaata aatgtgcaac tcttgtttgc ttcatttcgt
     4081 ttgaagatag ttcacgccca tttttccaat gttgtgctgt aacaacctcg cctgtttcta
     4141 tgcgtttcgt ccatactaat ttgggcacaa taccttctgc tgataatgcc gcaataaatg
     4201 gatttgaatt tcgttttaaa aacaactttt gtccatcttg ttcagccata tatgcttcac
     4261 cagatgcacc acctgctgaa tcaagtgtcc accctaattg ataaaactgc tccaactcgt
     4321 ccacctcact ttcaattaga aaatggctct agaaataggt ttttcaagag ccatatattc
     4381 taatttataa caccatactg gtacaaatat tatgtccaga taattattgt aaatcctcaa
     4441 ccaatgccta cattacacga ctaaatttaa atcgtaatgt ctgtcattga caccatacat
     4501 tctatagtca cttacttgac atataatgtt accgtgtcta aaactacatg tttttgaatc
     4561 tctgtaggcg ataaactcta gttttcaaaa taattgctat cccattttca tggttagcat
     4621 aaatttatga actgtaacat ttacgtactt agtaaaatat gatgcacatc atatttgtaa
     4681 ctcatagaaa attttataat ttttatcatt atatttcaac tgaaaatgag aaacaaaatg
     4741 gcacttttta ctaatatgtg ttttctaaac aacactttta agcttcgttt taaattataa
     4801 cataattcac ttacgaaagt tgataaattt aagtaattta atctaaaaat atgatgaaag
     4861 aattttaaat actgtgtgac tctatatact tttcaaatcc ttcttgtagt tgacgtgtaa
     4921 ttgggccaac tttaccatca ttaactggtt caccatctaa tttaataaca ggtgtaacct
     4981 cagctgaagt acttgaaaca ataacttcat ctgcgttttt caagaaatct acagtaaacg
     5041 tttcttcttt aaatgggatg ttatagtctt cggcaatttt tttaattaca attcgtgtaa
     5101 taccattaag aatatagttg ttaatcggat gtgtataaat cacaccgtct ttaattgcat
     5161 aagcattact tgaagatcct tcagttacag tttcacctcg atgttgaatt gcttcaactg
     5221 cattatattt cacagcatat tcttttgcta atacatttcc taataagttc aagcttttaa
     5281 tgtcgcaacg taaccatcgg atatcttcaa cggtaacacc attcacacca ttttctaaat
     5341 gatcataagg acgatcataa ctctttgtat aagcaacaat tgctggttct acttcaggtg
     5401 tcgggaagct atgattcctt tcagctacac cacgcgttgc ttgaatataa attgccccag
     5461 tttcaatttg attcatatca actaatttac gagatagttc aattaattct tctacagaat
     5521 aatttaaatc taaaccaatc tcattggcac tacgtaaaaa tctttcataa tgttctgtta
     5581 ctgtaaataa cttaccatta tatactcgaa tgtattcata aataccatcg ccaaatacgt
     5641 atcctctgtc gttgtatgaa acctttgctt cacttggact tacaaactca ccatttaaaa
     5701 aaattttttc catatattat tcctccacgc ataatgaata aattgcttct aagtaaatac
     5761 tagttgcgtt aaataactgt tttttagtga tatattcatt tttctgatgc attaaatctt
     5821 cagaatcact aaacattgcg ccaaatgcta cacccttgtc taagtttctc gcataagtac
     5881 cgccacctat agtataaggt tcagtcatat catttgtttg atttctatat gcagtaacta
     5941 acttttgtac aaaaggatca tttttatcaa cataatgtgg tggttggact ttacctaatt
     6001 tcacttcaaa gccatattgt tgaatctcat ttgcaaaacg atccatagct ttttcaaatt
     6061 caaatccttc tgggtagcgt aagttgatac cgaaaagacc tgcgttttca ttatcatatg
     6121 taataacacc aatgttagtt gtcacgtcac ccatgacatc tgtatggaat ttcattccca
     6181 tcttttcacc aaaatctgaa ttaaataagt agcgattact aaatgctaca aacgcttgtg
     6241 cattattatc aagatttaat gatgctaaga attttagtaa gtaaagaccc gcattcacac
     6301 cgatagatgg atccatacca tgaaccgctt taccttcaac tgttaaaact agaatgccac
     6361 tatcaacagt actatcacct tgtaaatgat tttgttctaa aaagtactca aagtcttgaa
     6421 taacatctgt catattttct ttaacaagca ctcttgcttc tgcatgatca ggtaccatgt
     6481 tgtaacgttc accagattta aaagttatta attcataatc aggttcatct tgatcttcag
     6541 taagtttatt ttgaactaaa tcaaatgttg taatgccttt ttcaccatga atacatggaa
     6601 attctgcatc tggtgcaaaa cctaatgttg gcatttcttc tgttttaaaa tagcgatccg
     6661 tacatttcca atcagattct tcatccgtac caataatcat atgaatacgt ttcttccaat
     6721 ccacattcat atcttctaat atcttaattg cataataagc agcaattgtt ggacctttgt
     6781 catcaagtgt acctctagct atgatagcat cttctgttac aaccggctcg aacggattac
     6841 tatcccatcc atcaccagca ggaacaacgt caacatgaca taagatacct aatacgtcat
     6901 ttcctttacc tgcctcaatt cttcctgcaa tatgatccac atcatgtgtt gtaaatccat
     6961 ctctatgtgc aatttcatac atgtagtcta atgccttacg aggacctgga ccaactggtg
     7021 cgtcttctga tgcttttgca tcatctctca cactttcaat tgctaataat ccttttaagt
     7081 cattaatgat ttgatcttcg tattgttgaa ctttttcttt ccacattcga aatcgacttc
     7141 cttttttcta taagttaaat tctattttac atgaaaagat ataaaaacta caataagatg
     7201 tcagaaaata ataaaaagga acaaaacgat gctattgata tgacacaaat cataaatagc
     7261 tgctttgttc cttttttaat ttatatattt aaaatacaca tattcaagag ctcgagatat
     7321 aagtcaatgt actaggcaca caatttaata ttgacagtaa ttaaccgaac gaaaatgcgc
     7381 cccggggccc caacatagag aatttcgaaa agaaattcta cagacaatgc aagttggcgg
     7441 ggccccaaca tagaagctgg ccaatagtta gctttcaata atgtgcaagt tggggtaagg
     7501 gccccaacac agaagctggc caatagtcag ctttcaataa tgtgcaagtt ggggtaaggg
     7561 ccccaacaca gagaatttcg aaaagataaa gaaatacgtt ttctttagat attagtattt
     7621 cttatgaatg agtttcacgc atgtattctt ctttctatat gcatattagc tatgactaac
     7681 gataaagaac ctgaaacact aataaatgtc ctatagttta caatattata ttggcagtag
     7741 ttgactgaat gaaaatacgc ttgtaacaag cttttttcaa ttctagtcaa ccttgccggg
     7801 gtgggacgac gaaataaatt ttgctaaaat atgatttctg tcccactccc ttatcatttc
     7861 tgtcctactc acatcttatt ctttatcaga taatgcattt ttattctttt ttaaatcttc
     7921 ttcagtgacg atacgtaaat tattatttgg tgctgcgcca ccttcatcat caaatttacc
     7981 tttttcaata ctttcgtcag tcttattgtc atattcggta aattttgatt tttcttcttt
     8041 gaaaaatgct tttggattat tttttaatct attagcatat tctttcggat ttgtttttac
     8101 ttctttaatt gtttcattag caattgttcc taattgcgtc gctttatcct tagcattatc
     8161 tttatagctt tgaggatctt gtttatattt attatattct gctttcagct tgtcacgact
     8221 atctttacgt gtaacaagta cagctgctac agcgccacct atacctaaaa tcgctttaaa
     8281 taaattacct tttgccatat caatcgtctc ccttttattt ataatttaat ttgtcaaaat
     8341 cattttcagt taataaacga tattctcctg aatctaaatt gctgtccaat tctaaatcag
     8401 caattttgat acgtcttaaa tgtaatacct cattttgaat gctatgaaac attcgtttaa
     8461 cttgatgata ttttccttca taaattgtta cgtgtgacgt ttgattatca atataagtta
     8521 atattgcagg cttaaccttg ccatcagtca gtgttacacc ctctttaaaa gcttgaatgt
     8581 cgtcttcagt gataggattt gctgaaataa cttcatattt tttagaaaca tgtttgtttg
     8641 gactcattaa ttcatgatta aaatcaccat cattcgttat caataaaagc ccttctgtat
     8701 ctttatcaag acgaccaacc ggaaaaatat ttagatgttg gtattcaggt attaaatcaa
     8761 taacggtttt tgaatgatga tcttcagttg ctgatatata accttttggc ttatttaaca
     8821 taatatagac attttcaatg tattctatta attctccacg aactgttatc ttatcgtttt
     8881 ctggttctat atgtgttttt ggtgatttaa ttacttgttc gttgacattt acaaggcctt
     8941 ttttaagtaa ctgtttgacc tcattacgtg taccgacgcc catatttgct aaaaatttat
     9001 ctattctcat cgtaaaaacc taactctacg tcttaatttt tcaggaattt cacctaagaa
     9061 ttcgtccgca agacgcgttt taattgtgat tgtaccgtaa attagaatac ctactgtaac
     9121 acctaaaata ataatgatta agtaaccaag tttagtaggt tctaagaata gatttgcaag
     9181 gaaaaatact aattctacac ctagcatcat aataaatgaa tacaagaat
//
LOCUS       DAKTLH010000104         9219 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.104, whole genome shotgun sequence.
ACCESSION   DAKTLH010000104 DAKTLH010000000
VERSION     DAKTLH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9219
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.104"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(261..1013)
                     /locus_tag="O0394_001989"
     CDS             complement(261..1013)
                     /locus_tag="O0394_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HCY0500059.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRIVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHIINTNEFDQFFAIS"
     gene            1181..2167
                     /gene="pta"
                     /locus_tag="O0394_001990"
     CDS             1181..2167
                     /gene="pta"
                     /locus_tag="O0394_001990"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCY0500060.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLDLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            2170..3006
                     /locus_tag="O0394_001991"
     CDS             2170..3006
                     /locus_tag="O0394_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCY0500061.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFENENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            3593..4513
                     /gene="mvk"
                     /locus_tag="O0394_001992"
     CDS             3593..4513
                     /gene="mvk"
                     /locus_tag="O0394_001992"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCY0500062.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            4518..5501
                     /gene="mvaD"
                     /locus_tag="O0394_001993"
     CDS             4518..5501
                     /gene="mvaD"
                     /locus_tag="O0394_001993"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCY0500063.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            5514..6590
                     /locus_tag="O0394_001994"
     CDS             5514..6590
                     /locus_tag="O0394_001994"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCY0500064.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEETDQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            6767..7108
                     /locus_tag="O0394_001995"
     CDS             6767..7108
                     /locus_tag="O0394_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HCY0500065.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKVEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(7160..8482)
                     /locus_tag="O0394_001996"
     CDS             complement(7160..8482)
                     /locus_tag="O0394_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCY0500066.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSPKMYGGTCI
                     NIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNLLDQHDDIVDSITAPHIIINTGATSVIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAYGITDLENKGIAL
                     HTNVETTELSSDNHHTTVHTNVDNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF"
     gene            complement(8469..8909)
                     /locus_tag="O0394_001997"
     CDS             complement(8469..8909)
                     /locus_tag="O0394_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCY0500067.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMIDTIRGKDGGYLANDQSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNIIINFYNGKTIKDVIEDIQKEDLCHENI"
     gene            8967..9068
                     /locus_tag="O0394_001998"
     CDS             8967..9068
                     /locus_tag="O0394_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500068.1"
                     /translation="MCQYLCLKSVELGVVIVMIYCLKVDALTVRLHN"
BASE COUNT         3195 a         1354 c         1768 g         2902 t
ORIGIN      
        1 ttgaggttaa cgtttatatg tagtgatagt agttaaagtt ctcccaagga agactactcg
       61 ggtacacttt gctatgagca aagtgtactt tgttattgat aatacattag cacatatata
      121 taagtttaaa catacagatt tcaactattt accaaatcac gtcttatgta tacgggaata
      181 tactgaagat aaacgaaaaa ttccaagctt aaaccgataa gcttggaatt tttttattaa
      241 tttataaacg taccaatgta ttaagaaatc gcaaagaatt gatcgaattc gtttgtgtta
      301 ataatatgtc ctacaaagaa actaccgaat tcaccgtatc gtgctgttgt ttcatcaaag
      361 cgcatttcgt atacaatttt tttgaattgt aatacgtcat ctgagaacaa tgttacgccc
      421 cattcgaaat catcaaaccc tacagaacca gtaataaatt gtttgatttt gccagcatat
      481 tttctaccaa tcataccatg gtcatacatt aatttttggc gttcttccat agttaacatg
      541 taccagttat aagtttcatt acgacgtttg ttcattggat agaaacaaat ataatcagaa
      601 tgtggtaatt ctgggtataa tcttgctttg atatgagggt tctcataagg atcttcatca
      661 gatttaccag ctaaataatt gctcaattca atgactgata catatgaata tgtagggatt
      721 aggaagtcag caatgcgcaa tttgttaaat tcattttcaa tatgatttaa agacttcatt
      781 tcaggacgta agaaccataa taacaaatct gctttttgac cagttatatt ataaatagct
      841 tgatcaccag attttgatga tcttacagtt gctgtatttt ctaaaaatga ttgaaattca
      901 gtgacaagtg catcgcgttc gtcctttgga actatacgta atgatgccca atcaactgca
      961 taaaataaat gtagactata ccaaccatct aatgtttcgg ctgcttgact catgtttatc
     1021 gctccttttc aaaaatcatc agttctttat aaattttatc ataaagcaaa ggggtaacga
     1081 taaaattatg attacaaatt ggtgacgtgg cattatgaaa taaaatggcg tataattata
     1141 ccgtgaatga ttaataagat ttatattaca ggaggacatt atggctgatt tattaaatgt
     1201 attaaaagac aaactttctg gtaaaaacgt taaaatcgta ttacctgaag gagaggacga
     1261 acgtgttcta acagctgcaa cacaattaca agcaacagat tatgttacac caatcgtgtt
     1321 aggtgatgag actaaggttc aatctttagc gcaaaaactt gatcttgata tttctaatat
     1381 tgaattaatt aatcctgcga caagtgaatt gaaagctgaa ttagttcaat catttgttga
     1441 acgacgtaaa ggtaaagcga ctgaagaaca agcacaagaa ttattaaaca atgtgaacta
     1501 cttcggtaca atgcttgttt atgctggtaa agcagatggt ttagttagtg gtgcagcaca
     1561 ttcaacaggc gacactgtgc gtccagcttt acaaatcatc aaaacgaaac caggtgtatc
     1621 aagaacatca ggtatcttct ttatgattaa aggtgatgaa caatacatct ttggtgattg
     1681 tgcaatcaat ccagaacttg attcacaagg acttgcagaa attgcagtag aaagtgcaaa
     1741 atcagcatta agctttggca tggatccaaa agttgcaatg ttaagctttt caacaaaagg
     1801 gtctgctaaa tcagacgacg tgacaaaagt tcaagaagct gtcaaattag cacaacaaaa
     1861 agctgaagaa gaaaaattag aagcaatcat tgatggcgaa ttccaatttg atgctgcgat
     1921 tgtaccaggt gttgctgaga aaaaagcgcc aggtgctaaa ttacaaggtg atgcaaatgt
     1981 ctttgtattc ccaagtttag aagctggtaa tattggttac aaaattgcac aacgtttagg
     2041 tggatatgat gcagttggtc cagtattaca aggtttaaat tctccagtaa atgacttatc
     2101 acgtggctgc tcaattgaag atgtatacaa tctttcaatt attacagcag cgcaagcctt
     2161 acaataacga tggatttagc gagtaaatat tttaatggcg tcaactggcg atatatcgat
     2221 cattcttctg gattagaacc tatgcaatct ttcgcattcg atgatacatt ttgcgaaagt
     2281 gtgggcaaag atatatcaga taatgttgtg cgtacttgga ttcatcaaca tactgttatt
     2341 cttggtattc atgattcaag attgccgttt ttaaaagatg gcattgatta tttaacgaat
     2401 gagattggtt ataatgccat tgttagaaat tctggtggct taggtgtcgt tctagatcaa
     2461 ggtgtattaa atatatcgct gatgttcaaa ggacaaacag aaacaacgat tgatgaagcg
     2521 tttactgtga tgtacctctt aattagcaaa atgttcgaaa atgagaatgt tgatattgat
     2581 acgatggaaa ttgaacattc ttattgccca ggaaaatttg acttaagtat cgatggtaag
     2641 aaatttgcag gcatatcgca acgaagagtt agaggcggta ttgctgtaca aatttatctt
     2701 tgtgttgaag gctctggttc agaacgtgca ttgatgatgc aaacatttta tgaacatgct
     2761 ttaaaaggtg aagtgactaa atttaaatat cctgaaattg aaccatcttg tatggcctca
     2821 ttagagacat tgcttaacaa aacgattact gttcaagatg taatgttttt actattatat
     2881 gcaatcaaag atcttggcgg tgtattaaat atgacgccaa ttactcaaga agaatggcaa
     2941 agatacgata cgtattttga taaaatgatt gaaagaaaca agaaaatgat agatcaaatg
     3001 caatagttaa atatttaaca gcccctttca agttatcgtt tggggctgtt atttttgaaa
     3061 gtatccataa aaagcataaa tatgttattg acctaatatt taaattgaac atcattaaat
     3121 taaaagtaat gatatgatgg aaataataaa tttaataaga aaatttcatg ttttacgtat
     3181 taaatagatt gagaataggg tatatataat gcgtatgact atgaaagaaa cgaccttcta
     3241 ttgaaatgtt ctaatacttt agtagtaaat ctatatattc caattattat ttgtagtatt
     3301 atagttaggg tggaaaatta taaattgtag ggctatttta taaaaagata aagaatctga
     3361 aatatcactc gataagattt aaactttgaa gtaaacgaaa gttagtttta tactaatcaa
     3421 gttaagtcaa atataaattc aaaataaaga ttatacataa tatgaataat gtgagataaa
     3481 gactgcaatg aaatttaacg agaatgcgta tgcaacttag aatatgatgt cttttactaa
     3541 taaataaaaa taatgaatca agtataggat aaatataaaa ggtgatattg acatgacaag
     3601 aaaaggatat ggggaatcga caggtaagat tattttaata ggagaacatg ctgttacatt
     3661 tggagagcct gctattgcag taccgtttaa cgcaggtaaa atcaaagttt taatagaagc
     3721 cttagagagc gggaactatt cgtctattaa aagcgatgtt tacgatggta tgttatatga
     3781 tgcgcctgac catcttaagt ctttggtgaa ccgttttgta gaattaaata atattacaga
     3841 gccgctagca gtaacgatcc aaacgaattt accaccatca cgtggattag gatcgagtgc
     3901 agctgtcgcg gttgcttttg ttcgtgcaag ttatgatttt ttagggaaat cattaacgaa
     3961 agaagaactc attgaaaagg ctaattgggc agagcaaatt gcacatggta aaccaagtgg
     4021 tattgatacg caaacgattg tatcaggcaa accagtttgg ttccaaaaag gtcatgctga
     4081 aacgttgaaa acgttaagtt tagacggcta tatggttgtt atagatactg gtgtgaaagg
     4141 ttcaacaaga caagcagtag aagatgttca taaactttgt gaggaccctc agtacatgtc
     4201 acatgtaaaa catatcggta agttagtttt acgtgcgagt gatgtgattg aacatcataa
     4261 ctttgaagcc ttagcggata tttttaatga atgtcatgcg gatttaaagg cgttgacagt
     4321 tagtcatgat aaaatagaac aattaatgaa aattggtaaa gaaaatggtg cgattgctgg
     4381 aaaacttact ggcgctggtc gtggtggaag tatgttattg cttgccaaag atttaccaac
     4441 agcgaaaaat attgtaaaag ctgtagaaaa agctggtgca gcacatactt ggattgagaa
     4501 tttaggaggt taatgcgttg attaaaagtg gcaaagcacg tgcacatacg aatattgcac
     4561 ttataaaata ttggggtaaa aaagatgaag cactaatcat tccaatgaat aatagcatat
     4621 ctgttacatt agaaaaattt tacactgaaa cgaaagtcac ttttaacgac cagttaacac
     4681 aggatcaatt ttggttgaat ggtgaaaagg ttagtggcaa agaattagag aaaatttcaa
     4741 aatatatgga tattgtcaga aatagagctg gcatcgattg gtatgcagaa attgaaagcg
     4801 acaattttgt accaacagca gcagggttgg cttcatcggc aagcgcatat gcagctttag
     4861 cagcagcttg taatcaagcg ctagacatgc agctgtcaga taaggattta tcgagattgg
     4921 cgcgaattgg ttcgggttct gcgtcgcgta gtatttatgg tggatttgca gaatgggaaa
     4981 aagggtatag tgatgagacg tcatatgccg ttccacttga atcgaatcat tttgaagatg
     5041 accttgccat gatatttgtt gtgattaatc aacattctaa aaaggtacct agtcgatatg
     5101 gtatgtcatt gacacgaaac acatcaaggt tttatcaata ttggttagat catattgatg
     5161 aagatttagc tgaagcaaaa gcagcgattc aagacaaaga ttttaaacgc cttggtgaag
     5221 taattgaaga aaatggtttg cgtatgcatg ccacgaatct aggatcaaca ccgccgttca
     5281 catatcttgt gcaagaaagt tatgatgtca tggcgcttgt tcacgaatgc cgagaagcgg
     5341 ggtatccgtg ttattttaca atggatgcgg gacctaatgt gaaaatactt gtagaaaaga
     5401 aaaacaagca acagattata gataaattat taacacagtt tgataataac caaattattg
     5461 atagtgacat tattgccaca ggaattgaaa taattgagta aggaagagat aaaatgattc
     5521 aggtcaaagc acccggaaaa ctttatattg ctggagaata tgctgtaaca gaaccaggat
     5581 ataaatctgt acttattgcg ttagatcgtt ttgtaactgc tactattgaa gaaacagacc
     5641 aatataaagg taccattcat tcaaaagcat tacatcataa cccagttaca tttagtagag
     5701 atgaagatag tattgtcatt tcagatccac atgcagcaaa acaattaaat tatgtggtca
     5761 cagctattga aatatttgaa caatacgcga aaagttgcga tatagcgatg aagcattttc
     5821 atctgactat tgatagtaat ttagatgatt caaatggtca taaatatgga ttaggttcaa
     5881 gtgcagcagt acttgtgtca gttataaaag tattaaatga attttatgat atgaagttat
     5941 ctaatttata catttataaa ctagcagtga ttgcaaatat gaagttacaa agtttaagtt
     6001 catgcggaga tattgctgtg agtgtatata gtggatggct agcgtatagt acttttgatc
     6061 atgaatgggt taagcatcaa attgaagata ctacggttga agaagtttta atcaaaaact
     6121 ggcctggatt gcacatcgaa ccattacaag cacctgaaaa tatggaagta cttatcggtt
     6181 ggactggctc accggcgtca tcaccacact ttgttagcga agtgaaacgt ttgaaatcag
     6241 atccttcatt ttacggtgac ttcttagaag attcacatcg ttgtgttgaa aaacttattc
     6301 atgcttttaa aacaaataac attaaaggtg tgcaaaagat ggtgcgtcag aatcgtacaa
     6361 ttattcaacg tatggataaa gaagctacag ttgatataga aactgaaaag ctaaaatatt
     6421 tgtgtgatat tgctgaaaag tatcacggcg catctaaaac atcaggcgct ggtggtggag
     6481 actgtggtat tacaattatc aataaagatg tagataaaga aaaaatttat gatgaatgga
     6541 caaaacatgg tattaaacca ttaaaattta atatttatca tgggcaataa atgatttgat
     6601 aggggagcaa tcatctaatt taattagaag ataaatgctc cattttttat tgtcagccgt
     6661 gttacgtatc ttgaaaaaaa tattaaaaag agtaaaatag atagagttaa cgaaaaaatt
     6721 aatgaaatcg acgatataga tatgataaag aaaggtgggt agcaatatga aaaatacatt
     6781 ccttatttgt gatgaatgtc aggcagtcaa tataagaacg ttacaaaaga agttggaaaa
     6841 attagatccc gatgctgaaa tcgtgatagg ttgtcaatct tattgtggac ctggacgccg
     6901 aaaaacattc acttttgtta ataaccgccc actggctgcg cttactgaag aagaattaat
     6961 cgaaaaagtt tctcaacaat taaagaaacc acgtgatcct gaagaagaag agcgtttaag
     7021 aaaacgacat gaagaacgta aacgtcgtaa agtagaacaa gatagaaagc ttaaagaaaa
     7081 attagaaaag cgaaaagcac aacaataaag cctgatggca gcatcattca atgcgtgcca
     7141 ccaggttttt atgttttgtc tagaaattaa ataaatcatt aaatgattcg gccatcgtag
     7201 gatgcgtata aatattatct cgtaatacgg tatatggaat gttttgatca atcgcaagtt
     7261 taattatatt aattaattct tcagattgct taccatataa tgtagcacct aaaatcatat
     7321 tattttcatt attaatgact actttaaata aacctcttgg atcattgtta attttatgac
     7381 gaggtatagc acttactaaa agttgatgtt cagtgtaatc ataatgttga gcggcagctt
     7441 ctttactagt taatccaaca cgtgataatg gtggatctat aaatactgta taaggcacgc
     7501 tgcctctatt gtcagtcgta cgtgactgat taccatataa cgctgatttg ataattcgat
     7561 aatcatctaa agatatatac gtaaattgaa gtccgccttt aacatcacct gcagcataaa
     7621 tatgcggcac agttgtttga agatgagcat tgactttaat ttcgcctctg tcgcctaatt
     7681 cgatatcagt attttctaaa gctaaatccg tattcggttt gcgcccgata gccaaaagta
     7741 ctgcatcagc ctcaaagtta tcaacgttgg tatggactgt tgtatgatga ttgtcagatg
     7801 acaattcagt cgtttcaaca tttgtatgca atgcaatgcc tttattttct aagtcagtaa
     7861 taccatatgc aacgacatct tgatcttcgc gtggcataaa tgattcgcca cgttctaata
     7921 ctgttacctt actacctaaa ttcgcaaaca ttgaagcaaa ttctaaggcg atataaccgc
     7981 cacctacaat aacgaggtgc ttaggttgat agctaatgtt taataaacct gtcgaatcga
     8041 agacgtgttt agcttgatca aggcctttaa tgttaggaat gacagaggta gcaccggtat
     8101 taataatgat atgaggtgca gtaatactat cgacgatatc gtcatgttga tctaataaat
     8161 tcacttcagt attagattta aactgcgctt taaaatccag tacatcaatg ttgttatcgt
     8221 ctgctaataa gtggtaattt ttattgttta gcgcattgac aacatcgttt ttacggttat
     8281 aacttgcttc aaaagatttg ccttctaatc catcatgtac aagtgtcttc gaaggtatac
     8341 atcctatgtt tatacaagtg cctccataca ttttcggaga ttgttcgata actgcgacgt
     8401 gttgacctgt tgatgcagcg tatttcgcta aagttttacc agctttccca aatcctatta
     8461 caattaaatc atatgttttc atgacataaa tcctcctttt gaatgtcttc aatgacatct
     8521 ttgattgttt ttccattata aaaattaata atgatattct gttcgtcttg ctgataatgt
     8581 gacatggtag ttgcaatatt acgagcaatt tgacagtgac tgccttcgtc gccagtaaat
     8641 agacgtgtgt ggtgttcttt ctctaagaca aaatgtttat ataatgttgc tagagagaca
     8701 tcagcacttt gatcatttgc taaataaccg ccatctttac ctcgtattgt gtcaatcatt
     8761 tttaaatcga caagttgagt cgtcacgcgt cgtaattgaa caggatttaa acaagttaat
     8821 tctgctaatg aactactatt gaatttttct gaatgatgct tagttaaaaa agctaataca
     8881 tgcacggcaa tgttaaattc taaattcaat gtttctcacc tcataattgt aactgtatta
     8941 gttacaatta aaaatcattt tgctcaatgt gtcaatactt atgtttgaaa agtgttgaat
     9001 taggtgtggt tattgtgatg atttattgtt tgaaagttga tgcattaacg gtgagattgc
     9061 acaattgaaa ttttaattat gtatgaactg atagatatga aatgaatatt gaaaatgaga
     9121 gcaataaaat tttgaatgat gatgttgtgt gattgtataa acttattcgt attttagata
     9181 cgttttgata tttcgcatga tgaggaaata cttttaaca
//
LOCUS       DAKTLH010000105         9132 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.105, whole genome shotgun sequence.
ACCESSION   DAKTLH010000105 DAKTLH010000000
VERSION     DAKTLH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.105"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(289..1497)
                     /locus_tag="O0394_001999"
     CDS             complement(289..1497)
                     /locus_tag="O0394_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCY0500069.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
     gene            complement(1490..1657)
                     /locus_tag="O0394_002000"
     CDS             complement(1490..1657)
                     /locus_tag="O0394_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500070.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            complement(1632..2483)
                     /locus_tag="O0394_002001"
     CDS             complement(1632..2483)
                     /locus_tag="O0394_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCY0500071.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHF
                     EERINQS"
     gene            complement(2983..4443)
                     /locus_tag="O0394_002002"
     CDS             complement(2983..4443)
                     /locus_tag="O0394_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCY0500072.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
     gene            complement(4693..7095)
                     /gene="parC"
                     /locus_tag="O0394_002003"
     CDS             complement(4693..7095)
                     /gene="parC"
                     /locus_tag="O0394_002003"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCY0500073.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDFSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            complement(7095..9086)
                     /gene="parE"
                     /locus_tag="O0394_002004"
     CDS             complement(7095..9086)
                     /gene="parE"
                     /locus_tag="O0394_002004"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCY0500074.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
BASE COUNT         3026 a         1711 c         1202 g         3193 t
ORIGIN      
        1 aaagaaaatg atgaatcaat acttatgaaa aatattatta attaacagcc gattatgagt
       61 gcttcttaat atattaaaaa taccacttat ttttattttc gacataataa tgaagtacta
      121 atcaagtggc aaatactcgt ttcattatgc atgtgcttta taaacggcta aactttcgta
      181 aaaatttata agctaaacat gtgctgataa tcataaatgt aactgcccaa cattataagc
      241 accataaaaa ataccaacaa acatatagtc attggtattt tttattactt attcaactat
      301 tttattactt tctttaatat catattcacc tacatcatta ccatagaaac gattgtatcg
      361 acgtttaaat aattggaata aatgagtaac taatactttt aaaatagcat atcccggaat
      421 acctaaaata acgcctacaa taccaagcaa tttgcctgca cacagtaaaa tgaaaatgat
      481 tgtaagtgga tgaatcttaa gtgttttacc catgatattt ggtgaaatga agtgcccttc
      541 aacaaattgt actaaagtcc atactactgc taattttaag agcatccacg gcgatgttat
      601 agcagctatt acaatagctg gagaaatcgc tatagtaggc cctaaatatg gtacaacact
      661 tgtaactgcc gcaatactag ctaatactaa gctatatttc aacccgataa ccgaataacc
      721 gataaacaac agtataccga tacagaatga aacgataatt tgtccttgaa tgtatgaacc
      781 aacttgaaca ctcatttttt caagtagatc atgaaaatct tttcggaatt tcggtggcat
      841 aatattcgtt gaaaattctt tgaagtgatg tccatcttta agcataaaga atagtacaaa
      901 tggtgttgtg acaatcacaa cgccaatatt agcaatcgtt tctgcaaacg ttgcaatttt
      961 agacccaaag ccatctgtaa attcacttaa catagatggt attttcttag gtaaagaatt
     1021 taaccaatca ttaatttgac tataatacga agagaaaaac gtattatttg ctattttatc
     1081 aatagaatta attaattttt ctagatattg cggtgaattt ttaactaaac tatctacttg
     1141 cgaaccaata ataggtatca ataaattaac aattaacgta ataacaccta ctacagctag
     1201 ataaataata gaaatacctg caactcttgg tattctataa cgttccatca tatttacgat
     1261 tgggttaaat agatagaata gaattaaaga gacgatgata ggtgctgcta tcgtcttaaa
     1321 aacgatgata aaaggatcaa aaacatatga tactttttcg aaaataaaaa taacaatacc
     1381 caacaatact agcgctatta atgagaagac taaatcattc ccaccaacaa acttcatata
     1441 ccgtgattcg gtaaatttta aaaaattctt tcttatattc ttttcatttt cattcaatta
     1501 tagattcacc accacttaaa atattcataa gatgttgtat aaaaacagtc cacttatgta
     1561 aaattactaa catcaatgat accatacaaa taccctttac agtaacgaaa cgcccgtgta
     1621 atacacttaa tttaggattg attaatcctt tcttcaaaat gatgaatgtg taatgttaaa
     1681 tatataattt cagactcact gatattaaca tcaaattgtt tttgtatcat agttaaaatt
     1741 ttatatgctg tgttatagca aatcggatag tgatttttaa tcatagacac aaaatcatct
     1801 tgtgcatgta tatattcttt tcttcttaat cggcgaatta aaaattgtac gtgccttata
     1861 aaacgttggt attgtaatga ttccttatca acagttgtca caaggtctga ttcaatgata
     1921 tctatacctt ttttaataac attattgatc aaggtcatct catgcataga taaatcttct
     1981 gtattagatg caatatgtaa tgcaataaat cctatctcat cttcaggaaa atgtacatct
     2041 aatgctgcat ttaactgatc aatcacctgt ttagcaatat gatatgcatc actatataac
     2101 tgcatagttt ccataacaaa tggattgcta ataacttgat tttgttttaa gcgtttataa
     2161 gcaaatataa tatgatccgt taatgaaact acaagttgtt ttgaatcaac attcatcgca
     2221 gtattagaaa taaaattcaa cgaatcaata attacttgta atacattatc atcagcgatt
     2281 tcaactaaac ttttataatg tgctttttgt tgctcacttt ctaatttata aattttctct
     2341 attgtaatag tttggtcgtt taacgccatt ccctcttttt tgttaaaacc aataccttta
     2401 ccgattaaaa taacttcttg atcattatta gtacatacta cgacattgtt gttcaatgtt
     2461 ttagtaacaa tatattctcc cattatcatc acctattttt ctattattaa gattatatat
     2521 cggaaatgtc taacttgaaa gtataataat ttaaatactt gtgttctatt acacgcctat
     2581 cctatatgat atatcttaat ttaatttcga tgtctctcaa agtggaataa ctataataaa
     2641 aaaatctgac tcccatattt acaaataatt ctatttattt atatattatc tgaattaata
     2701 ctcaatacaa actaaaacgt actattaaat tgtgcaaagc taaaacaaat ttatattcat
     2761 ctatccaaca atatgtctta tcaatggtat agtctttgca caccaatgga ggaaataaat
     2821 ctcaacctta ctatattaat atataatcaa atcttagatt aactagtgta atgatacaga
     2881 tgataattga gtacaaattt aaaaccctga gattttcgct ttaatttgaa aacctcaggg
     2941 tttatttgat ttttatataa tgaatcgtta cattaaaaat atttatttat cagagttctt
     3001 atatttgtta gcgccccaag cactaattcc aaataagtta atttctaagt tttcaggttt
     3061 aaagacaggg ttcttgcctt cttttttctg cttttgataa tctttcatca atgcaaaagc
     3121 tacattggac agtcctataa tggaaataat gtttacaatt gccattaagc ccataaataa
     3181 gtctgccgta ttccatactg tttctgtttt tacaactgca ccgacaaaga caagtactac
     3241 aacaagacat ctaaagataa ataatattac acggtttgtt gataaaaatt caatattaga
     3301 ttgaccgtaa tagtaattac ctacaacaga tgaaaatgca aacagtgtaa ctgctattgt
     3361 taagaaaata cctccagcag aacctaaatg ctcattaagt gctgattgag taactgcaac
     3421 accttgaggt gcgttatcac caaatttcag tcctgaatat agtaaaatca tgattgcagt
     3481 tgctgtacaa accaacattg tatcaaagaa cacacctaat gattggatta aaccttgctt
     3541 aacagggtgt ggtacggcag cagttgccgc tgcattcggc gcagaaccca taccagcttc
     3601 gttagagaat aaaccacgtt tgataccttg aagaaccgca gcacctacag cgccaccagt
     3661 tacttgttcg ataccaaatg cacttttaat aatcgtacct atcataggaa caatttgatc
     3721 taaattaaat agcaatatta ctaaaaccat accaatgtaa atgatagcca taatcggtac
     3781 aattaacgaa gataacgtag caatactacg tacaccacca aatataataa tagctgttac
     3841 gattgctaaa ataatacctg tgattactgg actaatatta tattgcgtat ttaacgactc
     3901 cgcaattgta ttagattgca ctgtgttaaa tacaaatgca aatgtaattg taattaaaat
     3961 cgcaaatacg atacctagcc atttttgatt taaaccttta gtaatatagt aagctggacc
     4021 accacggaat ccaccatctt tatcatgtac tttataaacc tgagccaaag tcgcttctat
     4081 aaatgcactc gctgcaccta taaatgcaat aacccacatc caaaatactg cacctggacc
     4141 gcctaaaaca atcgcagtcg caacaccagc aatattacca gtaccaactc tcgaaccagc
     4201 actaatcgca aatgcttgga atggcgaaat acccttctta ccatcttcta aagtttctgg
     4261 acgttctact aaagctctaa acatttcagg taacattcgt aattgaacga atttagaact
     4321 aatcgtaaag aagaatccag ctgtcaataa tagaccaatt aaatattgag accatattaa
     4381 atcggtacca acatggacaa attctttaaa ccatccaggt attaaactat cgaaatcttt
     4441 caaaataaac ccctcgcatc ctctacatga atcatgtacc ttctataaaa ttagaccgaa
     4501 ttgaaccttc agtaaatata gagatacatc atcatttctt atacaataca agagatttat
     4561 attagtttgg tcaaagtata tcgctaattt aacgataagt acttggttag catttaatat
     4621 aaatcccttg aatttagtca aaatttaaca ttactgtatt ttatcattta atttcgtgat
     4681 tgcatatagt ttttagctaa tatacatgtc tattacttca ccaaaatcat ctgtatctac
     4741 aatgaatgag ccatttgtat attgttcaga tttatgaata tcattaatta aaccatgttc
     4801 ttcatttgat tttgaatata atgtatattg actatgttca cctgtcacta catgtgcagc
     4861 tacaatacga tgtggatttt tctttaattc ttttaataaa gttattccac gttgtgctct
     4921 tttagcaact tgtaagattt taaaactaat acgttttaac gagccgcgtt gtgtggccat
     4981 caatatagta tcattttcag aaacaccttc tgtcataaca acgaaatctt cagctttaag
     5041 atttattgat ttaacaccag ctgcccttaa tccggtatct gatagttcac ttgtattata
     5101 cgttaatgac atacctttat tagtaatgac ggtaattaat tgatcttttt caaagcgcat
     5161 aacactaatc aaatcatcat tttctttaac tttagtagca attaaaggtt tattaaaacg
     5221 cgttgtttta aatagaggca ctgtactttt cttaatcatg ccattttgag tcgcaaaaac
     5281 ataaaatgca tctgtattaa agtccttttc attaaagaca ttaataacca cttcatcttc
     5341 ttcgatagga actatttgtg atacatgttg tcccaattct ttccaacgaa tatctgctaa
     5401 tttatgaacc ggtataaata gataacgacc tttatttgta aatactagta cggtatcttg
     5461 cgtatttact tcttgatgtt taagtaaact gtcaccatct tttaaaccaa tatcttcaac
     5521 accgctagca ttaaagctac gaatagaagt acgtttaata tatccatgac gtgtcatact
     5581 taaaataact tcttcactag gcaccataac ttctttgtca attttaattt cttcaatttc
     5641 tgcttcaatt aaagacagtc gttcagattt gaatttcttt ttaatttcat tcaattcttc
     5701 ttttatgaca ttcaataatg catcatggtt atcaagaata tgacgtaatt gtttgattaa
     5761 tgcttcaagt tctttatgtt caccttcaag cgcaactatg tcagtatttg ttaaacgata
     5821 taactgtaac attacaattg cttcagcctg ttcttctgtg aactcgtata cttcgataag
     5881 gttttcttta gcgtcacgct tgtttttaga gctacgaatc aattcgatta ctttatctaa
     5941 aattgacaac gctttaatca aaccttcaac gatatgcata cgtttttctg cattatctaa
     6001 ttcaaacttc gttctatttg caacaacctc aatttggtga ttcaaataac tatctataat
     6061 ttgacgaata cccatcaatt ttggacgacc atcactaata gcgaccatgt tgaaattata
     6121 tgaaatctgt aaatcagagt ttttataaag ataatttttg attgattcac tgttcacatc
     6181 ttttttcaat tcaattgcta ttcgtaaacc agttctatca gtttcatcac gtacttcaac
     6241 gataccatcg acttttttgt cagcacgtaa ttcatcgata cgttttacta agctactttt
     6301 gttcacttca tatggaattt cagtaataat taactgttta cgtccattgc gtaaagtttc
     6361 ttcttcaact ttagaacgaa ctataattct acctttacct gattcataag cttttttaat
     6421 accatcaata ccttgaataa taccaccagt tggaaaatca ggacctttaa tatatttcat
     6481 taattgattg actgtaatat ccggattatc aatatattta agtgttgctt gaatcacttc
     6541 agctaaatta tgtggtggta tatctgtcgc gtaacctgca gatatacctg tagaaccatt
     6601 cactagtaag ttaggaaatc ttgatggcaa taccattggt tcgagtgtcg tatcatcata
     6661 gtttggaatg aaagaaactg tctctttatt aatatcacgt aataactctt cagctagtaa
     6721 gcttaactta gcttcagtgt aacgcattgc cgctggcgga tcattatcga tactaccatt
     6781 attaccatgc atttctatta agacatgtcg taacttccag tcttgactta aacggaccat
     6841 tgcttcgtac actgagaagt ctccatgtgg atgatattga ccaataacat caccgactgt
     6901 tttcgcactt ttacggaaat ttttatcgtg tgtattacca cttgaataca ttgcatataa
     6961 aatacgacgt tgtactggtt ttaaaccatc acgaacatct ggcaatgcac gctcttgaat
     7021 aatatattta ctatatcttc caaagcgatc acctaaaaca tcttcaagtg ataaatcttg
     7081 aattatttca ctcactagat ttcctcctca tcaaattgat cattttcaag cacttgtact
     7141 tcagaattat ctaaaatact ttggtcctct tgcataccaa actcaacatg cttttcaatc
     7201 cattcacgtc taggttgtac tttgtcaccc attaatgttg ttacacgttt agatgaacgc
     7261 acttcatctt caacttgtac acgaattaaa gttcgtgttt ctgggttcat cgtcgtttcc
     7321 cataattgtt cagggttcat ttcacccaaa cctttgtaac gttgtaacgt gaagccttta
     7381 ccaagttctt tttgcaattt attaagctct tcgtctgtcc aagcgtattc aactcgcttt
     7441 gttttgcctt tacctttttc caatttataa agtggaggta aagcaataaa tacacgacct
     7501 gcttgaacaa gcggtttcat atatttgaag aagaatgtta acaatagcac ttgaatatgc
     7561 gcaccatcag tatcagcatc agtcataata attacacgat tataattact atcttcaatt
     7621 ttaaagtcag taccaacgcc tgccccgatt gtgtggataa ttgtattaat ttcttcattt
     7681 ttaaaaatat cttctagacg tgctttctct gtattaatta ccttaccacg taatggtaat
     7741 atcgcttgga atttgcggtc tcgtccaagt tttgctgaac ctcccgcaga atcaccttcg
     7801 actaaataca attcattttt ttcagtgttt ttactttgtg caggtgttaa tttaccagat
     7861 agcaaagtgt ctttacgctt gtttttctta cctgaacgag catcttcacg agctttacgt
     7921 gcagcttccc ttgcttgttg tgctttaatc gcttttttca caagtgattt agacaattgt
     7981 cctttttctt ctaaatagaa tggcaatttg tctgcaacaa ctgaatcaac agcacttcta
     8041 gcttcagaag tacccaattt agatttcgtt tgtccttcaa attgcaataa ttcttctgga
     8101 atacgaacag acacaacagc tgttaaacct tcacgaatat cattaccatc taagttttta
     8161 tcttttgttt taagttcatt aatacgacgt gcataatcat taaatacacg tgtcattgct
     8221 gttttaaaac caacttcatg tgtaccacca tctttagtac gtacattatt tacaaaactt
     8281 aaaatacttt ctgaatattg atcattatat tggaaagcta cgtctacctc tataccattt
     8341 gcttcacctg aaaatgtagc cacgtcatgc aaaacttctt ttccttcatt gacataacta
     8401 acaaactctt tgattccttc ttcataatgg taatgctctt gacgctcttt accactgcgt
     8461 aaatcattaa gcgttatttt taaatttttc aataagaacg cagactcttg tagtcgttca
     8521 cttaaaacat caaaattaaa tgatgtagat gctttaaaaa ttgtgtcatc aggtttaaat
     8581 gttactttgg tacctgtttt cttagtttta ccttttttca ctaaaccaga agatggcgaa
     8641 ccaccgtttt taaaactttg atgatatata ttaccatctc gatggatttc aacttcaagc
     8701 cattcactca atgcatttac aactgaagca ccaacaccgt gaagaccacc tgaagtttta
     8761 tagccgcctt gtccaaattt acctcctgca tgtaaaacag taaagataac ttcgactgtc
     8821 ggtttacctg atttatgtat acctgttggc ataccacgtc cattatcttc tatagaaata
     8881 ctaccatctt tattaattgt tacatctatt tcgttaccgt aaccattcaa tacttcatcg
     8941 acggagttat cgacaatttc atatactaga tgatgtaatc cccgtttatc agttgatcca
     9001 atatacatac caggtctttt acgaactgct tctaacccct ctaaaacctg tattgaatca
     9061 tctgaataat tattttgttt attcattgcc aatgatttcg cctcctgcaa acgtacgttc
     9121 gtttttaaaa ct
//
LOCUS       DAKTLH010000106         9089 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.106, whole genome shotgun sequence.
ACCESSION   DAKTLH010000106 DAKTLH010000000
VERSION     DAKTLH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.106"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            36..560
                     /locus_tag="O0394_002005"
     CDS             36..560
                     /locus_tag="O0394_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCY0500075.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFCKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRNLRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
     regulatory      547..644
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            719..1810
                     /gene="gcvT"
                     /locus_tag="O0394_002006"
     CDS             719..1810
                     /gene="gcvT"
                     /locus_tag="O0394_002006"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCY0500076.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     gene            1830..3176
                     /gene="gcvPA"
                     /locus_tag="O0394_002007"
     CDS             1830..3176
                     /gene="gcvPA"
                     /locus_tag="O0394_002007"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HCY0500077.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            3169..4641
                     /gene="gcvPB"
                     /locus_tag="O0394_002008"
     CDS             3169..4641
                     /gene="gcvPB"
                     /locus_tag="O0394_002008"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HCY0500078.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSKHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(5006..5392)
                     /locus_tag="O0394_002009"
     CDS             complement(5006..5392)
                     /locus_tag="O0394_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCY0500079.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            5550..6380
                     /locus_tag="O0394_002010"
     CDS             5550..6380
                     /locus_tag="O0394_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCY0500080.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIEFKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            complement(6444..6662)
                     /locus_tag="O0394_002011"
     CDS             complement(6444..6662)
                     /locus_tag="O0394_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500081.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLISFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(6676..7257)
                     /locus_tag="O0394_002012"
     CDS             complement(6676..7257)
                     /locus_tag="O0394_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500082.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            7362..8423
                     /locus_tag="O0394_002013"
     CDS             7362..8423
                     /locus_tag="O0394_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCY0500083.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDADEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            8449..9006
                     /gene="efp"
                     /locus_tag="O0394_002014"
     CDS             8449..9006
                     /gene="efp"
                     /locus_tag="O0394_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCY0500084.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
BASE COUNT         3230 a         1234 c         1679 g         2946 t
ORIGIN      
        1 taagattgtt ttgttattga aagaagggaa aacgcatgaa tcatgataaa tcaccaataa
       61 ttttaattgg tttcatgggt accggtaaat ctacgattgg taaatacgtt gcagatgagc
      121 aaaatttatc atttattgat atagattcat atatcgaaga gaagtataag ttaacaatac
      181 cagaaatatt ttgtaaacat ggtgaacaat atttcaggaa tttagagttc acatgtttgc
      241 aagaatgtat taacactgca gatataattg ctactggtgg tggtattatt gagagtgaag
      301 aggcatttaa ttttttgaaa aatcaaaaaa acattatttg gttagattgt aatattgata
      361 ttatatatag tcgaatcaat gatgacccac atcgacctaa tgcaaataat aagacaatca
      421 agcagttaaa tgacttgtat tgctcgcgga atttaagata taatgaaatc gcattcaaga
      481 aatttgatag tcatttgcta tcaatttcag aaatatatta tgaattgcta aatttaataa
      541 aagcgagtga tcagtattag agagaataga gcgttaagac tctatcgccg aaggtgcaag
      601 taatttatta cgaaactctc aggcaaaagg ataatactgt aacgcgttcc tgaattggtg
      661 atttataaac agggtagcga ttgctatcct gtttttataa ttttaagggg gtatttcaat
      721 gtcaagtgat ttaaaacaaa cacctttata tcaaaattat gttgatagag gtgcaaaaat
      781 tgtggaattc ggaggatggg cgatgcctgt tcaattttca agtattaaag aggagcataa
      841 tgctgttcga tacgaaattg gcctgtttga tgttagtcat atgggtgaaa ttgaagtaac
      901 aggtaaagat gctagtcagt ttgtgcaata tttattatca aatgatactg ataatttaac
      961 tacttcaaaa gcattatata ctgctttatg taatgaagaa ggcggtatta ttgatgattt
     1021 agtaatatat aaattagctg acgacaatta tttattagtt gttaatgctg ctaatactga
     1081 aaaagatttt aattggattt taaaacacaa agagaaattt gatgttgaag tacaaaatgt
     1141 atcaaaccaa tatggtcaat tagcaataca aggaccaaaa gctagagatt taattaatca
     1201 attagttgat gaagatgtaa ctgaaatgaa aatgtttgaa tttaaacagg gtgtcaaatt
     1261 atttggagca aacgtcattt tatctcagtc aggttacaca ggtgaagatg ggtttgaaat
     1321 ttattgtaat atcgatgata ctgaaaaaat ttgggatggt ttattagagt ataatgttat
     1381 gccatgtggg ttaggcgctc gtgatacatt aagattagag gctggattgc cattacatgg
     1441 acaagattta actgaatcaa ttacaccata tgaaggtggt atcgcttttg caagtaaacc
     1501 attaattgat gctgatttta ttggtaaatc tgtattaaaa gatcaaaaag aaaatggtgc
     1561 accaagaaga acagtgggat tagaattact tgaaaaagga attgcaagaa ctggttatga
     1621 agttatggat ttagatggaa atattattgg agaagtaact tcaggaacac agtctccatc
     1681 atcaggaaaa tcaattgcac ttgcaatgat aaaaagagat gagtttgaaa tgggtagaga
     1741 gttgcttgtt caagttcgta agcgtcaatt aaaagcgaaa attgttaaga aaaatcaaat
     1801 tgataaataa ttaaaaaggg gtgtgcattg tgagtcatcg ttatatacct ttaactgaaa
     1861 aagacaagca agaaatgtta caaacaattg gtgcaaaatc tataggagaa ttattcggtg
     1921 atgtaccaag tgacatttta ttaaatagag atttaaatat tgctgaaggc gaagcggaaa
     1981 caacgttact tagaagatta aatcgcattg caagcaagaa tatcactaaa gaaacgcata
     2041 catcattttt gggtgctggt gtttatgatc attacgcgcc atcagttgtg gatgctatga
     2101 tttcaagatc agaattttac acagcatata cgccatatca accggaaatt tctcaaggtg
     2161 aattacaagc catttttgag ttccaaactt taatttgtga attaacagat atggatgtag
     2221 caaattcttc tatgtatgat gggatgacta gctttgctga agcatgtatc ttagcattta
     2281 gtcaaacaaa gaaaaataaa attgtagtct caaaaggttt acattatcaa gcattacaag
     2341 tacttcatac ttatgctaag acgcgtaagg aattcgaagt agtggaaatc gatttagatg
     2401 gcactgttac agatttgaaa aaattagaag cagcagtgga tgatgaaaca gcagctgttg
     2461 cagtacaata tccaaacttt tacggttcaa ttgaagatct tgaaaagatt catagcttta
     2521 ttgaagataa aaaagcatta tttatcgtat atgcaaatcc attagcattg ggattactta
     2581 cacctccagg ttcatttggt gcagatattg ttgtaggtga tactcagcca ttcggaattc
     2641 ctgctcaatt cggtgggcca cattgtggat attttgcaac gacgaaaaag ttaatgcgta
     2701 aagtgccagg tagattagtc ggtcaaacac aagatgatga aggtaatcgt ggctttgtat
     2761 taacattaca agcacgtgaa caacatattc gacgcgataa agcgacatct aatatttgtt
     2821 ctaaccaagc attaaatgct ttagcgtcat caattgcaat gtcagcactt ggtaaacaag
     2881 gtatctatga tattgcagtt caaaatatag aacatgctaa ctatgctaaa caacaattta
     2941 ttaaaaaagg ttttgaagta ttagatggta catctttcaa tgagtttgta gtcaaatttg
     3001 acaaaccaat tcaacaagtt aacgaggaat tagtgaagta caatattatc ggtggttttg
     3061 atttaggtgt tgtttcagac gactttaaaa atcacatgtt aatagctgta actgaattaa
     3121 gaacaaaaga tgaaatcgat acatttgtag aaaaggctgg tgagttaaat gactagtaaa
     3181 tcaagtccat taatttttga aagatctcgt gaaggcagat atgcatattc attaccaaaa
     3241 agtgatatta aaacaaattc tgttgagtca ttgttagatg ataaatttat tcgtaaaaac
     3301 aaagcggagt ttcctgaagt tgctgaacta gacttagtac gtcattatac tgaactttca
     3361 aataaaaatt tcggtgtaga caacggattt tatccattgg gttcatgtac gatgaagtat
     3421 aaccctaaaa ttaatgaaaa ggttgctaga ataccaggat ttagtgaatc acatccatta
     3481 caagatgaag atcaagttca aggttcatta gaaattattt atagtttgca agaagaatta
     3541 aaagaaatta ctggtatgga tgaggtgaca ttacaaccag ctgctggcgc acatggtgaa
     3601 tggactgcat tgatgatatt taaagcttac catgagaata atggtgaagg tcatcgtgat
     3661 gaagtcattg tgccagattc tgcgcatggt acgaatccag cctcagcttc atttgcagga
     3721 tttaaatcag ttactgtaaa atcaaacgaa cgtggcgaag ttgatattga tgacttgaaa
     3781 cgtgttgtaa atgaaaatac agcagctatt atgttaacta atccaaacac tttaggtatt
     3841 ttcgaaaaaa atattatgga aatccgtgaa atcgtccata atgctggtgg tctattatat
     3901 tatgatggtg cgaatttaaa cgctattatg gacaaagttc gcccaggaga tatgggattt
     3961 gatgctgttc atttaaactt gcataaaaca tttactggtc cacatggtgg tggcggtcct
     4021 ggttcaggtc cagtcggtgt agtaaaagaa ctagcaagtt acttaccaaa gccaatggtt
     4081 attaaagatg gcgacaaatt taaatatgat aatgacatta aaaattctat cggacgtgta
     4141 aaaccatttt atggtaactt tggtatttac ttaagagctt atacgtatat tcgaactatg
     4201 ggagcaactg gacttaaaga ggtttctgaa gcagcggttc ttaatgcgaa ttatattaaa
     4261 gcacgtttat ctaaacactt tgaaatacct tataaacaat attgtaaaca cgagtttgtg
     4321 ttaagtggtg tgcgtcaaaa agaatttggt gtacgtactt tagacatggc taagcgatta
     4381 ttagatttcg gtgtacatcc accaacaata tacttcccat taaatgttga agaaggtatg
     4441 atgattgaac cgactgagac agagtctaaa gaaacacttg attattttat cgatacatta
     4501 attagtattg ctgaagaagc taaaaatgat cctgataaag tgctagaagc accacataca
     4561 actgtgattg atcgattaga cgaagctaca gctgctcgta aaccaatatt aaagtttgaa
     4621 aatcttaaac aggaaaaata aagtattaaa cacatattcc gagaattatt attctaactt
     4681 tgtatgaaga tttaaggata atggtttcaa aatcaattga aaaagacaat ttctatttaa
     4741 acaagaaaac taaaccgaag taataactct tagggtttgg tattattctt tcatagaaat
     4801 tgtcttttca ttttttagat tgcggtaatt gaatcgtatt gaaaatgagc tgaactttct
     4861 ttattatgct gaaactaagt ttaatgatca gtgaagctag agaaatatag taacagactt
     4921 tgttaaaaaa ttaaaatctg agtattgtta ttgatgatca attaacactc agattttatt
     4981 aaatcgtata ttaaatttac aaaaactatt ttttagactt tatttttcca gtccattttt
     5041 tatagccgcc ttttaacata tagatatctg tatatccatt ctttttcaaa atacgagcgg
     5101 ctctatagct agcaatccca ttggcatcac ataagtatac cggttgatct tttcttaatc
     5161 cttggaatcg ttgcctgaac attgtcatag gaatattgcg agacccatta atgtgaccgt
     5221 agtcatagtc aactttctct ctaacatcga tgacttgagc ttttctaatc ccattatgga
     5281 attcattttg atctaattct ttaacagctc gcttgttaag aatttgttga acaatcatat
     5341 aagcaataat tgctataact aaaattattg cgatgtacaa actagcactc atcttgcatc
     5401 ctccctaatt aaacgataat ataattataa gactgttagg tcaaattatc aaaatatttc
     5461 actaaagaat tgcaattatt attcatttag ttaaattgat taattagcga atcaaatatt
     5521 atacattttg ctgataggag ttcttgaata tgactgaaac ttggaatttt attaatacag
     5581 gaagtaaaga cccatattat aatatggcaa tggatgaagc gttattaaat tttgtctcta
     5641 gaggtgaaat tgatccagtt atacgttttt acacatggaa tcctgcaaca ttatcaatag
     5701 ggtatttcca aaggctacaa aaagaaattg atattgataa ggttaaagag aaaggttttg
     5761 gtctcgtaag gcgtcaaact ggtggccgtg gggtgctaca tgataaagag ttaacttaca
     5821 gtgtgattgt gcctgaatcg catccgaata tgccttctac agttacagaa gcgtacagag
     5881 tgatttcaca aggattatta gaaggattta aaaacttagg atttgatact tattttgcgg
     5941 tacctaaaac gcctgaagag cgtcaaaaat taaaacaacc tcgaagttca gtgtgttttg
     6001 atgcaccaag ttggtatgag ctagttgttg aaggccgtaa gattgctggg agtgctcaaa
     6061 cgagacaaaa gggcgtaatc ctccaacacg gttcgatttt acaagatatt gatattgatg
     6121 aactgtttga tatgtttatt tataaaaatg aaagattaaa attaaaaatg aaagaggcat
     6181 tcgttgaaaa agcagttgcc atcaatgata tttcagatga acacattact attagtcaaa
     6241 tggaagaagc tttcgagaaa ggttttaaga aaggtttaaa tatagaattt aagccacttg
     6301 aattaacaga agctcaattg gcagaagtgg aagaattaac tgaaaaatat cgttcagatg
     6361 aatggatgtt tagaaagtag tttttagaat aaaaaatgga agcacgtaga ctattttacc
     6421 tacaagtgct tccgtttttt tatctatttt cttctttgat ttcttttata cttacgcatt
     6481 gctttgcgat atttgcgttg gtcttcagtt aagatgaaga aataataaat cagataaata
     6541 ataccagcta ttacagcaaa gctgattaac attctgacga ttgaaaaaat aaaggcatct
     6601 agattcatta ctaatccaat cgcagcaata ataagtacaa gataaaatat tatattacgc
     6661 attaaaagac tcctatcatg attttgcatt taagtttaat tttgacaacg cttgttgtcc
     6721 ttttgtaagt tgttttttat ctttattttg atagccattt ttaatatcat caaatgcatg
     6781 attcaaatca gagtttaatt tatcgatatt tttagacgtt tttttatcat catcactaag
     6841 tgataaacca tcgatagcat ctttgtatga agtgtgagca ctttgaatat cattagaaat
     6901 ttgatttaat ttcgattgaa cattcttatc gcctttgttt ttacttacat ctttctcaat
     6961 atcgttatag gatttcaaag ctttagcaac tttgtcgtag tatgttgatg taacattcat
     7021 caatttaatt ttcgtattat tagatgcagt ttttttactg ttttctttat ccttttcaag
     7081 ccctttaatt ttagatttag aatcactaat ttgttgatta agttcttgaa tatctagttt
     7141 gagttgatga ttatcttctc ttaaatctgt tgttttttct tctaatggtg ctaaattttg
     7201 tgatccacat ccagctaaca ataatgttgc gccaacaatt gaaaccaatt ttttcattac
     7261 tatctcccta aaatcaatat ctattatttt ttatccttat tatgatatta ttttaatgta
     7321 taaagttgaa atgcacaaac gtttcactag gaggtaataa gatgagcaga ataacacaag
     7381 tgcatcgtat tttagaacaa aaacatttag atgcgattat cattctttcg gattacaata
     7441 gaagatattt atcaggtttt actggtacca gtggagcact tatcatatct aaagataaac
     7501 aatatttgat aacagacttt agatatattg atcaagcaac taagcaggcg ccaaattatg
     7561 aaattattaa tcgtaaatct actattattg gtgagattaa agaattgcta caccaagaaa
     7621 attttgaaaa tgtaggtttt gaggggcatc atgtaagtta tgatacatac cttgaattaa
     7681 ataaaagccg tatatcatta ataagcattt ctaatactgt agataaaatt agagacgtca
     7741 aagatgctga cgaaattgct ttaattcaaa aagcagctaa tattgttgat gaaacatatg
     7801 aatatatttt aactgttgta aaagcaggca tgactgaaaa agaattaaag gcaatattag
     7861 aaagcaaaat gctagaatta ggagcagatg gaccatcatt cgatacgatt gtagcatctg
     7921 gtcatagagg tgcattacca catggtgttg caagtgataa aattattgaa aaaggcgaca
     7981 tgattacatt agattttggc gcgtattata acggctattg ttcagatatt actagaacat
     8041 ttgctattgg agaaccagat cctaaactga aagaaatata tcaaatagta cttgaatctc
     8101 aaatgaaagc aattaatgag attagacctg gcatgactgg tgcagaagct gatgccattt
     8161 caagaaacta tttagagtca aaagggtatg gaaaagaatt tggacattca ctaggacatg
     8221 gtattggttt agaaatccat gaagggccaa tgctggctcg tacgatacaa gataaacttc
     8281 aagttaacaa ctgtgttaca gtagaacctg gtgtttatat agaaggtttg ggcggtataa
     8341 gaatagaaga tgatatttta attacagaaa atggttgtca agtctttact aaatgcacaa
     8401 aagaccttat agttttaaca taagcgtgta aaatgaggag gaaactgaat gatttcggtt
     8461 aatgatttta aaacaggttt aacaatttct gttgataacg ctatttggaa agttatagac
     8521 ttccaacatg taaagcctgg taaaggttca gcattcgttc gttcaaaatt acgtaattta
     8581 agaactggtg caattcaaga gaaaacgttt agagctggtg aaaaagttga accagcaatg
     8641 attgaaaatc gtcgcatgca atatttatat gctgacggag ataatcatgt atttatggat
     8701 aatgaaagct ttgaacaaac agaactttca agtgattact taaaagaaga attgaattac
     8761 ttaaaagaag gtatggaagt acaaattcaa acatacgaag gtgaaactat cggtgttgaa
     8821 ttacctaaaa ctgttgaatt aacagtaact gaaacagaac ctggtattaa aggtgatact
     8881 gcaactggtg ccactaaatc ggcaactgtt gaaactggtt atacattaaa tgtaccttta
     8941 tttgtaaacg aaggtgacgt tttaattatc aacactggtg atggaagcta catttcaaga
     9001 ggataatctc taatttgtta acaaatagct tgtattcact atactgattt aacgtaagat
     9061 attctaaata agtctcataa agctattgc
//
LOCUS       DAKTLH010000107         9036 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.107, whole genome shotgun sequence.
ACCESSION   DAKTLH010000107 DAKTLH010000000
VERSION     DAKTLH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9036
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.107"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..712)
                     /locus_tag="O0394_002015"
     CDS             complement(<1..712)
                     /locus_tag="O0394_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0500085.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKR"
     gene            complement(934..2283)
                     /locus_tag="O0394_002016"
     CDS             complement(934..2283)
                     /locus_tag="O0394_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCY0500086.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE"
     gene            complement(2504..3685)
                     /gene="nagA"
                     /locus_tag="O0394_002017"
     CDS             complement(2504..3685)
                     /gene="nagA"
                     /locus_tag="O0394_002017"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCY0500087.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEVDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDNALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            complement(3993..5951)
                     /locus_tag="O0394_002018"
     CDS             complement(3993..5951)
                     /locus_tag="O0394_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0500088.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKAGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDEADDEVTKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GANSFNPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YAFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(5957..6877)
                     /gene="pfkB"
                     /locus_tag="O0394_002019"
     CDS             complement(5957..6877)
                     /gene="pfkB"
                     /locus_tag="O0394_002019"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCY0500089.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            complement(6874..7638)
                     /locus_tag="O0394_002020"
     CDS             complement(6874..7638)
                     /locus_tag="O0394_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCY0500090.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            complement(7888..8370)
                     /gene="ybaK"
                     /locus_tag="O0394_002021"
     CDS             complement(7888..8370)
                     /gene="ybaK"
                     /locus_tag="O0394_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCY0500091.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            complement(8544..8996)
                     /locus_tag="O0394_002022"
     CDS             complement(8544..8996)
                     /locus_tag="O0394_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500092.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
BASE COUNT         2762 a         1789 c         1240 g         3245 t
ORIGIN      
        1 tgcgtttagg tgtctgagat tttggaataa taattctatt gtgtgctaaa tgccaacgta
       61 atacaacttg tgctggtgtt ttatgatatt tttcagcaat tttaacaatg acagggtcgt
      121 ctagtagtcc tctatttctc atcaaaggca tccatgcagt cactttaata tcgtgacgat
      181 cacaaaattc ttgtaattct tgttggttaa aatatggatg tacctcaatt tgattcacca
      241 ttggtttgat acttgattga gccattaatt tttctagatg atgaacatta aaattacata
      301 cacctattgc ttttacctta ccttgctcgt aaagttcttc catagcttta tatgtttcta
      361 aaaatagacc atctgcttca caaggccaat gtattagaaa taaatcaaga taatcagttt
      421 gtaaattttc aatcgatttg ttgaaatatt cgaatgtttt ctcataacct tgatagtcat
      481 tccataactt cgttgttata aacaaatctt ctctatcgac gccattatcc tttaatgctc
      541 gtcctagtga agcctcatta tcataaaagt atgctgtatc aaacgctcta tagcctgcgt
      601 caattgcagc atttacaact ttagtcatat cttcgtcaga gattttataa acacctaacc
      661 caactgaagg catcgggtat ccattattta atatttgtat ctcattcaac atctttatat
      721 ctctccaatc tatgtatctt ttatatcttt acattaccct aaatttttca acaaactcaa
      781 ttaatacgaa ttatcgcttt caataaaaat tattcattaa atcattaaag atattgagtt
      841 ccaatactat tttcactttt cattaatttt agtcaaaaaa ataaccaacc aaaaatgaat
      901 taaatcattc ttagttggtt tatatattaa tatctattct gatttttcat cttcatcaga
      961 ctgtccgata gtaggtctcg cttcattaaa ttcatagttt aatatcaccc aaataatttg
     1021 gtggttatcg atttctgaaa caacccagcg atcataagtt gtatccacgt aatcatcttt
     1081 ttgtaaattg gtattacgag attgtaacca tccacctatc gtatcaatat cctcagagtc
     1141 atcaaattct ataccgaact cttcagttaa atcatccaat agtactctgc catttacttg
     1201 gaatgtctta ttatcaattt taacgatatc attcacttca tcatcatcaa attcatcacg
     1261 aatttctcca acgatttctt ctaaaatatc ttccatcgtt aaaatacctg ccgttccacc
     1321 atattcatct ataataagac tcatatgtac atgttcacgt tgcattctaa ttaatgcatc
     1381 actgatacgt gttgtctctg aaatcattgg caactcatgt atatagtttg ctattttaat
     1441 cgtttttcca gaagcgtatt cagttaaaaa ttctttgacg ttaataaatc ctttaatgtg
     1501 gtctttatca ccatcatcag taattggata acgcgtaaat tgatgttctt ttattgtttc
     1561 tagtaattcg tctacattaa aaggttcatt tagtgtaatc atttgagttc taggtaccat
     1621 tatatctttt gcatgtcttt catcgaatga aaagatattt tgcatatatg ccaattcagt
     1681 ttggttgatt tctccaccat tataactatt gttaataata attttgattt cttcttctga
     1741 cattgcatca gtttgggcat caggatttac accaaacatt ctaataataa cacgtgcaga
     1801 accattcatc agccaaatca atggtttcat aatgttaccg aaatagaaca atggtcttgc
     1861 atatactaaa gcaagctttt cagtatgttg aatagctata gatttaggcg ctaattcacc
     1921 aagtactaca tgcaaatacg taacgattat aaatgacact gcaaacgaaa tcgtcgtcgt
     1981 taatgcagtt ggtaaattga ttgcttcaaa tattgggtgt aatagctttt caaacgttgg
     2041 ttcaccaagc caacctaacc ctaaagatgt tactgttata cctaactgac aagcagaaag
     2101 ataataatct agattagcaa tcatcttttt tactatttta gcaggtttat ttccttcatc
     2161 tgctagctgt tcaattcttg ttgctctaat ttttactaat gcaaattctg aaccaacaaa
     2221 tacagtggtt aatgcaatta atagaataaa tataatcaaa ctaattatgg tcgaagtttc
     2281 caattaattc cctatttcta gggattcacc tccatgttat atgtgtcact catgggtaac
     2341 acgcattcaa atttatcact atgacttaaa ttacaactac atgttatcgc cttcccattt
     2401 agacaccccc agaaaaaaat gttattgctt ctattttatc ataatataaa gtgcttatgt
     2461 taacagatta aatctattgc atacatttaa ttatgattat ttattagcta aatgtgtgaa
     2521 ccttgccttg ttttattgta gattttacat tcatatcatc atctagaata acaagatctg
     2581 catccttatt tactttaata ctaccttttc tatcatcgat acctaatgca atggcttgat
     2641 ttaaacttgt tactcgccat aaatgatcta atgtatcacc tgtaaatgat attaagttac
     2701 gtaacccatg attcattttt aaaatactac cagcaagcgc accatttgca agacgtgctt
     2761 gttgcgattg aacagttact ttttgtccac ccaaatcata ttctccttca ggcatacctt
     2821 ttgcacgcat tgcatcggta attaaataaa aacgttcatt acctttcata cggtaagcaa
     2881 ttgcaaccga tgccggatga gaatgagtgc catcaacaat catttcggta tgtagagcat
     2941 cattcaacca tgctgctcca aaaacacctg gttctctatg ttggaatggc gtcgctgcat
     3001 tatataaatg cgtgacatgt ttagctcctc gctcaacagc ttcaactgct tcttcgtatg
     3061 ttgctactgt atgaccaatt gaaaaaataa tgtcatcttt atacgtttca agcgcttctt
     3121 ttgcaccttc aacttcaggt gcaaacgtca taatctttat taatccgtta gcagtctctt
     3181 gaaaatgttt aattttatcg ataaatgggc gtacaacgta ttgcggatgt tgagcaccaa
     3241 ctttattttc agatataaat ggtccttcta aatgtatacc tacaatttcc gctgcattgt
     3301 gaacatcttg ctccgcttca tatttagcaa tatttgtaag tgcattatct attttatcag
     3361 tcgattgcgt cattgtagtg gccaagtatg atgtcgtccc ttcagacaac aaattttcgg
     3421 atagatattt taagccatcg tatgacccat ccattgcatc ttgaccataa ccaccatgaa
     3481 tatgtatatc aataaaacct ggtaatacat gatgaccttt agcatcaatc acttgaattt
     3541 tatttgtcgt atcattatca attgctgctt tatcatccac ttctccaatt gcaacaatct
     3601 gtccatcttt cacatgaatg taaccattat cgattttgcc atcttcagta taaactttgc
     3661 cgttatatat aattaattct gacacgtata aacccttctt tatttataaa ttttcacttt
     3721 aagacactca tattttacta tatctgcttc gtttggcata actttttgca aaatattaaa
     3781 aaagtctata catttgtggt gtctatttct tcattcattt atctaaaaat aaaaacactt
     3841 tcatatcaat tcataaaaac tgaattaata gaaagtgttt ttacagcttt gtataagcta
     3901 ccgttaacac gatatataaa actaacatat attcattgaa catacaactt aatatgaagc
     3961 tctttgctaa caatcatttt acatcattaa aactactcgt ccattgattt tgaagcttcg
     4021 atttctgttt cagttaactt tggtttgatt aaaccgtaaa ttaatgctga aactaatgtg
     4081 ccaactagaa gtgcaataag agtttgaagt aagtgtgcac catcagtacc aacaattaca
     4141 ataataccac catgtggcgc agtaattcgt gaacctaagc ctaaagcaat tgcgccacct
     4201 atacctgaac caatcatcat tgaaggaata acacgtaatg gatcggcagc tgcaaatgga
     4261 atcgcacctt ctgtaataaa tgacataccc atcacatagt tagggataat tgaaccacgt
     4321 tgttcttttg taaatttacg tctaaaaatt aacatcgctg tcgcaattgc taacggtgga
     4381 atcataccac caatcattgc agctgtaatt ggtgctgcat taccttcaat caacgcacct
     4441 gttgcaaaaa catatgccgc tttgttgaat ggaccgccca tatcaatcgc catcatagcg
     4501 ccaataacta aacctaataa tacaatatta gaacctgata aattgtttaa tccatctaat
     4561 aacaaatgat ttaaccaaga tgctggtgga ttaaaggcat aaatcatcaa taagcctgta
     4621 gccgtcactg ttaatagtgg ataaattaat gttggcttta atccctctaa tgcttgtggt
     4681 aacttacgtg tcatggcttt aacaccttgt gttaagtaac ctgctaagaa acctgcaata
     4741 ataccaccaa taaatcctga accacctgaa attgctaaca taccacctac aagacctgaa
     4801 gcgaaaccag gtttatccgc aatactacgt gcaatgaatc cagataaaat tggaatgatt
     4861 aacgcgaatg cacttttact accaatgttc caaagctgct ctgcaaacgc attgtactct
     4921 gagctttttg gattaaatga atttgctcca aataaaaata caattgccat taaaatacca
     4981 ccagatatta caagtggcaa catgttagaa acaccgttca ttaagtgttt atagaatgct
     5041 ttacctgggc ttaatttttc ttgactgtca tttgagttac cttttctttg accatcacgg
     5101 gcaacaaaag gtttacgact tgtatctaat gctttattaa ttaattcttc tgggcgttta
     5161 ataccatctg ctactggtac ttctacgaca tttttaccat cgaagcgatc cgtctcaaca
     5221 tgaacatcag cagcaacaat gatacctgtt gcattttcaa tatcttgttc agttaaatgg
     5281 tttttaatgc cgcttgaacc attcgtttct actttaattt taatacccat tttatccgct
     5341 tgctttttca atgcatcacg tgccatatat gtgtgtgcaa tacctgttgg acaagcagtt
     5401 actgctaaca catatggctc attagattgt ttagatgatt gttctgcagt tgcaacttgt
     5461 tgtgcttcag cttctgcctc ttcttctttt gtcacttcat catcagcctc atctatgatc
     5521 gctagtactt cttcaggtga tgaagcatgt aataatttct cacgtacatt ttcatccatt
     5581 aaaataccag acaacttagc taaagcatct agatgtgttt gggcgccacc ttctggcgct
     5641 gcaatcataa agaataagtg tgctggttgc atatccaaac tttgataatc tacgcctgct
     5701 ttagatttac caaacgcaat agctggtgac ttaactgcgg ccactttggc atgtggaatg
     5761 gcaatacctt cgccgatacc agttgtactt tgtgattctc gattgtgaat cgcttcctta
     5821 aatgacgcga catcacttaa tttacctgct ttgtctaatt gatttactaa ctcatcaata
     5881 acaccatttt tgtcatttgc cattaaatcc attgctattg tatcttttgt taataactct
     5941 gttactctca ttattttcac tccccatcaa gtacgctaat cgtaacttgt gattttattt
     6001 tttctatagc gtcccgtgtt gctaagtcct catcaaatgc cgtggcagta ccgcatgcga
     6061 ctgcttgttg gaatgctttt tcaatcgtta aacctgaagc aattccagcc accatgcctg
     6121 caactgtact atcaccagag ccaactgtat taaccacttt cccttgtgga ttaactgctt
     6181 taatactgat ttctttatca atataaatag caccatcacc gccaagcgag acaataacag
     6241 attgcgcacc tttatcaact aacaaacgac catatttaat aacatctgtg tctgagttca
     6301 ctgttgtatt aaacatcact tctaattcat ctttattagg tttaataaat agtggatgat
     6361 atggtaaaac gctttcagcc aattcttttt cagcgtcgac tactaattta gcacctgtct
     6421 gtgctgtaat ttgtgcaatt tgcgcatacg catcgcttgg aatactactt ggtacacttc
     6481 cagcaacaat aactatatct tcgcttgttg tatttttaat ttgttgtaac agttgttcaa
     6541 attgtgttga cgttatatga ggacccggtg cattgatttc tgtttcttgt cctgttttta
     6601 atttcacatt aatacgtgta tcttcatcaa cttcaataaa attcgattga attgcactgt
     6661 tatttaatgt atctataatg aatttcccag gaaatccacc tgcaaatccc aaggcagttg
     6721 actcaacatc caatgtcttt aagacgcgcg agacattaat acctttcccc ccagcgaatt
     6781 tatatgttgc tgttgctctg ttcaaaccat caattttaaa atcattcgta aaaatgacat
     6841 agtcaattga aggattgaaa gtcactgtat aaatcataaa gtccctccta taaagtgata
     6901 cttttgttgg tattctttta acgattcttg atttaatgct ttttcagatg tgatgattgt
     6961 cgtactttct agcaaaggta cacgagcaaa atatacttta ttaaacttag aatgatctat
     7021 aagtacaaat gattgattgg ctaatgacat tgctgtttgt ttaactaatg cctcttgctc
     7081 atcgggagta gttaatccaa gttcaatatc taatccattc atcccgataa aagctttatc
     7141 gaaacaatat cgtcttaata tctccatagc actagaacca atcgtagcaa gtgtattttc
     7201 tttaacttga ccacctagca taattgtttt aatacctttt ttaagtaaag cttctacatg
     7261 tgttaaacca ttagttacca caatgatatc tttcgcttga atatatttaa ttagctccaa
     7321 tgtagatgaa ccagcatcga taaataagca ttcattatcg ttgatttgat tagctgctat
     7381 tttagcaatc attttctttt catcaagatt cgttgctaat ttttcagtta aattcgcctc
     7441 aaccatacga ttttctttta acattgcacc accatgcaca cgttgcagtt tccctaattg
     7501 ttgtagttta gataaatctc ttcgtattgt tgaagcactg caaccagttc gatctattaa
     7561 ttcttgtaaa gtcaaaaaat ctttgtgcga aagttcttct aatattaact cgtgtctttt
     7621 ttcagtaatt atcatcatca ccccatctca ctttgtatta taatgagtac gctttcactt
     7681 ttcaatcata aattaccaaa aacaatcaca aacactcaaa ttgtgacgat aaaattacat
     7741 ttttatgttc aaatactcaa ttataatgac tgtttatgat cgtaaaatgc taattcaatc
     7801 aaaaaggagt gtggcagaaa tgaattttat aaaaatttca tctctgtcac actcctaatc
     7861 ttgccccata ctttttgttg ttattaatca ttcatggata actgctccaa ttttgccttt
     7921 agttattgta atcaaatcct caacagctat tgtgatttgc attgttcgaa gcccaccact
     7981 cacactgata tgactataat tttcacacga tttgtcaacg actgttggaa acaatgtttt
     8041 cataccaaca ggcgaacacc caccacgaat gtatcccgtt acatttttca aattatctaa
     8101 aggcataagc tgcaatttct tctctccaac caaagcagct gcctttttca tatctaaagt
     8161 ttcacttact gggataacaa atacaaaatg ttcatgtttt gtattttcta gaacaagcgt
     8221 tttaaatact gttttaacat ttgcttgtat gagttctgcg acttcttgac cagataaatg
     8281 ttcttctggt acctcaaagc tatgaacttc gtatttaatt tttgcacgat caagcatacg
     8341 catcgcgttc gttttcttat ttttagccat ttcaacacct catttatact tcttcagaac
     8401 tatattaaca ttttaaaata cattacggat tggttgattt tgacataaca tttacatcat
     8461 tttataagtt gaattcacct ttttaaatta aattattgca aaataaaaac agtatatcca
     8521 attagaatac actgttccat ctattatttc gttattatgc cttgatttga aatacttaca
     8581 tgttgcttac tagttgaatt atcattggaa tacacataat tatatccatg atttgtctta
     8641 gaaattcttt ctaaaccata attatcattg atatgcttat ctgcataacg atcagcaata
     8701 acttggcact cttgttcgtt aaaagaattt gatttatttg cattcacacg atgtttaaaa
     8761 tgattttgct ttttcacatt ttcattcact tcagatgctt tagcagtatg aatattgaga
     8821 atatccattt tctttttata agtatcttca gttgatacat ttatcgcgtt aagcgtatta
     8881 attgttttat gtaccttagc aagcgcatct tcatttgttg ctaatattaa taaaccaatt
     8941 aaacaaacgg ttaatattac agcaacaaag cctaaaaata aattttttac tgtcatcccc
     9001 ttacccacat ttccttactc attttttatc atctta
//
LOCUS       DAKTLH010000108         9011 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.108, whole genome shotgun sequence.
ACCESSION   DAKTLH010000108 DAKTLH010000000
VERSION     DAKTLH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9011
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.108"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..620)
                     /locus_tag="O0394_002023"
     CDS             complement(<1..620)
                     /locus_tag="O0394_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL8"
                     /protein_id="HCY0500093.1"
                     /translation="MKFTVIAKAIFILGILTTSVMITENQSVNAKGKYEKMNRLYDTN
                     KLHQYYSGPSYELTNVSGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSIDEFFFIQK
                     EEVSLKELDFKIRKLLIKKYKLYEGSADKGRIVINMKDENKYEIDL"
     gene            complement(964..1659)
                     /locus_tag="O0394_002024"
     CDS             complement(964..1659)
                     /locus_tag="O0394_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HCY0500094.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVQAKEKQERVQHLYDIK
                     DLHRYYSSESFEFSNISGKVENYNGSNVVRFNQENQNHQLFLLGKDKEKYKEGIEGKD
                     VFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFSINKEEV
                     SLKELDFKIRQHLVKNYGLYKGTTKYGKITINLKDGEKQEIDLGDKLQFERMGDVLNS
                     KDINKIEVTLKQI"
     gene            complement(2080..2775)
                     /locus_tag="O0394_002025"
     CDS             complement(2080..2775)
                     /locus_tag="O0394_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL6"
                     /protein_id="HCY0500095.1"
                     /translation="MKLKTLAKATLVLGLLATGVITTESQTVKAAESTQGQHNYKSLK
                     YYYSKPSIELKNLDGLYRQKVTDKGVYVWKDRKDYFVGLLGKDIEKYPQGEHDKQDAF
                     LVIEEETVNGRQYSIGGLSKTNSKEFSKEVDVKVTRKIDESSEKSKDSKFKITKEEIS
                     LKELDFKLRKKLMEEEKLYGAVNNRKGKIVVKMEDDKFYTFELTKKLQPHRMGDTIDG
                     TKIKEINVELEYK"
     gene            complement(3221..3925)
                     /locus_tag="O0394_002026"
     CDS             complement(3221..3925)
                     /locus_tag="O0394_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HCY0500096.1"
                     /translation="MKMTAIAKASLALGILATGTITSLHQTVNASEHKAKYENVTKDI
                     FDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKNRKFTRVQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIATYGRVHYI
                     YKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKIKVIMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
     gene            complement(4289..5215)
                     /locus_tag="O0394_002027"
     CDS             complement(4289..5215)
                     /locus_tag="O0394_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HCY0500097.1"
                     /translation="MKITTIAKTSLALGLLTTGVITTTTQAANATTPSSTKVEAPQST
                     PPSTKIEAPQSKPNATTPPSTKVEAPQQTANATTPPSTKVTTPPSTNTPQPMQSTKSD
                     TPQSPTTKQVPTEINPKFKDLRAYYTKPSLEFKNEIGIILKKWTTIRFMNVVPDYFIY
                     KIALVGKDDKKYGEGVHRNVDVFVVLEENNYNLEKYSVGGITKSNSKKVDHKAGVRIT
                     KEDNKGTISHDVSEFKITKEQISLKELDFKLRKQLIEKNNLYGNVGSGKIVIKMKNGG
                     KYTFELHKKLQENRMADVIDGTNIDNIEVNIK"
     gene            complement(5324..5461)
                     /locus_tag="O0394_002028"
     CDS             complement(5324..5461)
                     /locus_tag="O0394_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500098.1"
                     /translation="MFVKVAFLCLKSDETSNVPSVESHQNHFYLTNIMDFLIYLTMIQ
                     I"
     gene            complement(5580..6638)
                     /locus_tag="O0394_002029"
     CDS             complement(5580..6638)
                     /locus_tag="O0394_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HCY0500099.1"
                     /translation="MKMRTIAKTSLALGLLTTGAITVTTQSVKAEKIQSTKVDKVPTL
                     KAERLAMINITAGANSATTQAANTRQERTPKLEKAPNTNEEKTSASKIEKISQPKQEE
                     QKTLNISATPAPKQEQSQTTTESTTPKTKVTTPPSTNTPQPMQSTKSDTPQSPTIKQA
                     QTDMTPKYEDLRAYYTKPSFEFEKQFGFMLKPWTTVRFMNVIPNRFIYKIALVGKDEK
                     KYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVNHKVELSITKKDNQGMISR
                     DVSEYMITKEEISLKELDFKLRKQLIEKHNLYGNMGSGTIVIKMKNGGKYTFELHKKL
                     QEHRMAGTNIDNIEVNIK"
     gene            complement(6929..7624)
                     /locus_tag="O0394_002030"
     CDS             complement(6929..7624)
                     /locus_tag="O0394_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HCY0500100.1"
                     /translation="MKMKNIAKISLLLGILATGVNTTTEKPVHAEKKPIVISENSKKL
                     KAYYNQPSIEYKNVTGYISFIQPSIKFMNIIDGNSVNNIALIGKDKQHYHTGVHRNLN
                     IFYVNEDKRFEGAKYSIGGITSANDKAVDLIAEARVIKEDHTGEYDYDFFPFKIDKEA
                     MSLKEIDFKLRKYLIDNYGLYGEMSTGKITVKKKYYGKYTFELDKKLQEDRMSDVINV
                     TDIDRIEIKVIKA"
     gene            complement(7910..8590)
                     /locus_tag="O0394_002031"
     CDS             complement(7910..8590)
                     /locus_tag="O0394_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HCY0500101.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNVQSVQAKAEVKQQSESELKH
                     YYNKPILERKNVTGFKYTDEGKHYLEVTVGQQHSRITLLGSDKDKFKDGENSNIDVFI
                     LREGDSRQATNYSIGGVTKSNSVQYIDYINTPILEIKKDNEDVLKDFYYISKEDISLK
                     ELDYRLRERAIKQHGLYSNGLKQGQITITMNDGTTHTIDLSQKLEKERMGESIDGTKI
                     NKILVEMK"
BASE COUNT         2595 a         1584 c         1172 g         3660 t
ORIGIN      
        1 cttaaatcaa tttcatactt attttcatct ttcatattaa taacaattct acctttatca
       61 gctgaccctt catacagttt gtatttttta atcaacagtt ttcttatttt aaaatcaagt
      121 tccttcaatg acacttcttc cttttgaata aagaaaaatt catcaataga aaaaccgtct
      181 ttatcgtcta tttttttaac tagtaagttc ggcgttctta gagactcaaa tattgatttt
      241 acgtttttct ttgttacacc actaacacta aatattcttc catctaaatc tactaattct
      301 ggtaccgcaa agacatctaa accatgtgtt ttttctttgt atttattttc atcttttccc
      361 aataaaaaca cttggaactt ttgattttgt tggttaaaaa gcaaaacgtt agagtcataa
      421 taaccttgac tttggccact aacatttgtt aactcataac taggtcctga atagtattga
      481 tgtaacttgt ttgtatcata taaacggttc attttttcat actttccttt tgcattaacc
      541 gattgatttt ctgttatcat tacacttgtt gttaatattc ctaatataaa tatcgcttta
      601 gctatcactg taaatttcat attcatgtgc tccaatctta atatattgga ttgcttttat
      661 tacgtaattt gaataataca cctttattat aaagggcatt gagacagact gacattaaca
      721 ctcgtttaat taaacttaaa tcattgttaa tgaagagcta attaatattc aatgggcatc
      781 actttcaaga gcaatgtaaa tttttagtat gctcactcat aacctgcgtt ataaaacgta
      841 aaaaaaagcc taacgacaag gttgttttaa atacgctcct taatgtaagg tgtacgtatc
      901 tattcaacat ttcatcgtta agctgcttca aatttattac tttagagtca ttgattactt
      961 actttaaatt tgtttcaaag tcacttcaat cttattaata tccttactat tcaacacatc
     1021 acccatgcgc tcgaattgca atttatcacc taaatcaatt tcttgctttt ctccatcttt
     1081 caaattgata gtgatcttac cgtatttagt cgtaccttta tataaaccat aatttttaac
     1141 taaatgttgt ctaattttga aatcaagttc tttcagtgaa acttcttctt tattaattga
     1201 aaatgagtca attgatgcat ctaaatttcc gccatacact ttattaacaa ataaatgtgt
     1261 attagtttca gaagatttgt tatttttctt agtcacacca ccaacagtag ataatctacc
     1321 gtttggatca attaattctt ttaccacaaa gacatctttg ccttcaatgc cttctttata
     1381 tttctcttta tctttaccta ataagaataa ttggtgattt tgattttctt ggttaaagcg
     1441 tacaacgtta gaaccgttat aattttcaac cttaccacta atattactga attcaaaact
     1501 ttctgatgag tagtatcgat gtaagtcttt aatatcatat aaatgttgta ctctctcttg
     1561 cttttctttt gcttgaactg cttggccttc tgatgtaatc acaccagtag ttaataagcc
     1621 taatgccaat gttgctttag ctaacgtttt taatttcaca gtactattct cccaatctat
     1681 ttataaattt gtcttaatat atttttatat gattaattca attacgtaat acgaacaatc
     1741 tactatcatt atataagtga gatactgcgc tatgaattaa cctcttttta actatttttg
     1801 aaccaacgtt aagttagtta atgaaatcct aacagtatta attatttttt ataacacaat
     1861 actaattttt taactattca cactttccct caatttaatt atgttttcca cattgtttca
     1921 tgtcacgaaa aggacaacgc gcgactataa gtatcaaccg tttttccaaa aacttgatca
     1981 taaacccgct ccttttttca tcataacaaa ataaaaaaga agctaagcaa tatgttatcg
     2041 cttaacttcc ctacaattac tagtctgctt gtccaaagat tatttatatt ctagctcaac
     2101 attaatttct ttgattttgg taccatctat cgtgtcaccc atgcgatgcg gttgtagttt
     2161 ttttgtaagt tcgaaagtat aaaacttatc atcttccatt ttaactacaa ttttaccttt
     2221 tctattatta acagcaccat ataatttttc ttcttccatc aatttttttc ttaatttaaa
     2281 gtccaactct tttaacgaga tttcttcttt agtaatttta aatttactat ctttagactt
     2341 ttccgatgat tcatcaattt ttcttgttac tttaacatcg acttctttac taaattcttt
     2401 actatttgtc ttacttaaac caccaattga atattgtctt ccattaacag tttcctcctc
     2461 gatgactaaa aatgcatctt gcttatcatg ctcaccttga gggtattttt caatatcttt
     2521 accaagcaag ccaacaaaat aatcttttcg atccttccaa acatatactc ctttatctgt
     2581 cactttctgt ctatacaaac catcaagatt ttttaactct atacttggct tgctatagta
     2641 gtattttaac gatttataat tgtgttgacc ttgagttgat tctgccgctt ttactgtttg
     2701 actttctgtt gttattacac cagtagctaa caatcccaat actaatgttg ctttagctaa
     2761 cgtttttaat ttcatagtac tattctccca atctatttat aaattttgtc ttaatatatt
     2821 tttatatgat tatttcaatt acgtaatacg aacaatctac tatcattata taagcgtaat
     2881 actaagctat gaattaacct ctttttaact atttttgaac caatgttaag ctaattaatg
     2941 gaatcctaac agcgttaatc tattttttaa acttaacgca gcttttttaa ctattcacac
     3001 tttccctcaa tttaactatg ttttccacat tgtttcatgt cacgaaaagg acaacgcgcg
     3061 actataagta tcaactattt ccacaagttt tattggtgtt tttattattc atcgatacgc
     3121 ttcattttca tctctccaac acaaaaaaga agctaagcaa cttatgttgc ctaactcctc
     3181 tatactatcc atattttact attatccata tttcattgaa ttatctaatg ttggcttcta
     3241 ttttttcaat atttctaccg tcaatgacgt cactcatgcg atttgtttgt aattttttat
     3301 taagttcaaa cgtataatag ccgccatctt tcattatcac ttttatctta ctatctttag
     3361 gaaacttttt atacagatca aaattttgaa ttaaatactg tctcaattta aagtcgagtt
     3421 ctttaagtga aatctcttct ttataaatgt agtgtactct accgtacgta gcaataccgt
     3481 caccttcatc tctcttgatt tgaaatcttg gtgcgtttat ataatcataa taagcgtctt
     3541 gatttttctt agtgacacca ccatatgaaa acactgtgcc attacggttt tccgcttctt
     3601 taacaacaaa tatgtctaat cccggatttt tacgtgcttt aaatctttca atatctttac
     3661 caaatatctg tactcttgtg aattttctat ttttatcaaa gataaggtaa tgcttgccac
     3721 ctttgctata acgataacca gtaacatttt taagttcctt acttgcgcca ctatagtaat
     3781 ctcttaagtc aaagatatct tttgtcacat tttcatattt tgctttatgt tcactcgcat
     3841 ttacagtttg atgcaatgac gttattgttc ctgttgctaa aatacctaat gctaaacttg
     3901 ctttcgcaat tgctgtcatt ttcatagttg tatgctccat tcgtaattat tagatttgtt
     3961 cgcttacgtc tattgaatca tacagcttta ttatagttag cgtatttgac ctttcacatt
     4021 aaaccatgtt taataatcat tgaatcatta ttaagtaaat taaggaatct ataatgttcg
     4081 ttaaataaaa ctgatcccgt tgtgcttcac acccgataga tagggattta cagataaatt
     4141 caggtctctt ccacgtcata tttggaccca tcgaaaattc gggttctcaa atcatcgaac
     4201 ataacaaaag aagctaagca acatgtaggc cgttgtcact taacttcttg tttttccgat
     4261 gacagcttct atttagagaa tgtcatgatt attttatatt cacttcaatg ttatcaatat
     4321 tagtgccatc tatgacatct gccatgcgat tttcttgtaa ttttttgtgc aattcaaacg
     4381 tgtactttcc accgtttttc attttaataa caattttacc tgaaccaacg ttaccgtaca
     4441 gattattttt ttcaataagt tgttttctca atttaaaatc aagttctttc aaggaaatct
     4501 gttctttagt aatcttgaat tctgaaacat catgagagat tgtaccttta ttatcttcct
     4561 tagtaattct tactcctgct ttgtgatcaa cttttttact attactcttt gtgataccac
     4621 cgacagaata tttttccaga ttgtaattat tttcttctaa aacgacaaat acatcgacat
     4681 tcctatgtac tccttcacca tattttttat catctttacc aactaaagca attttatata
     4741 tgaaataatc tgggacaaca ttcataaatc ttattgtcgt ccattttttt aaaataatac
     4801 caatctcatt tttaaattct aaacttggtt tcgtataata cgctcttaaa tctttaaatt
     4861 taggatttat ttctgttggt acttgttttg tggttggcga ttgtggtgtg tctgatttag
     4921 tagattgcat tggttgtggc gtgtttgttg atggaggtgt tgtcacttta gttgaaggcg
     4981 gtgttgtcgc atttgctgtt tgttgcggtg cttctacttt agttgagggc ggtgttgtcg
     5041 cgtttggttt tgattgcggt gcttctattt tagttgaggg cggtgttgat tgtggtgctt
     5101 ccactttagt ggaagatggt gttgtcgcgt ttgctgcttg cgttgtcgtt gtgattacac
     5161 ctgttgttaa aaggcctagt gctaaacttg ttttagcaat cgttgttatt ttcatagttg
     5221 tatgctccat tcgtaattat tagatttgtt cgattacatt cattgaatca tacagcttta
     5281 ttatagatgg cgtattgctc cattcacatt aaaccttgtt taactatatt tgaatcatcg
     5341 ttaagtaaat taagaaatcc ataatgttcg ttaaataaaa atgattttga tgtgattcaa
     5401 cacttggcac atttgaagtt tcgtcacttt taagacatag aaatgccact tttacaaaca
     5461 aatgaatatt cgtcttttta catcattacg cataataaaa gaagctaagc aacatgtaaa
     5521 ccgttgtcac ttaacttctt gtttttccga tgacagcttc tatttagaga atgtcatgat
     5581 tattttatat tcacttcaat gttatcaata ttagtgcctg ccatacgatg ctcttgcagt
     5641 tttttgtgta attcaaacgt atatttccca ccgtttttca ttttaataac gattgttcct
     5701 gaacccatgt taccgtaaag attatgtttt tcaataagtt gttttctcaa tttaaaatca
     5761 agctctttca aggaaatctc ttccttagta atcatgtatt ctgaaacatc gcgtgaaatc
     5821 ataccttgat tatctttttt agtaatgctt aattctactt tgtgattaac ttttttacta
     5881 ttagtcttcg tgatgccacc gacagaatat tttttcaatt gatatttatt gtcttctaaa
     5941 acgataaata catcgatatt atcgtaaggt ccatctttat attttttctc atcttttcca
     6001 actaaagcta ttttatagat gaacctattt ggaataacat tcataaacct aaccgtcgtc
     6061 catggtttga gcataaatcc aaactgcttt tcaaattcaa aactcggttt tgtataatac
     6121 gctcttaaat cttcatattt aggagtcata tctgtttgtg cttgttttat ggttggagat
     6181 tgtggtgtgt ctgatttagt agattgcatt ggttgtggcg tgtttgttga tggaggtgtt
     6241 gtcactttag ttttcggcgt tgtggattcg gttgtcgttt gtgattgttc ttgtttaggc
     6301 gctggcgttg ctgatatatt aagcgttttc tgctcttctt gtttaggttg tgatattttt
     6361 tctattttgg aagctgaggt tttttcctca ttagtatttg gtgccttttc gagtttaggc
     6421 gtgcgttctt gtcttgtgtt agctgcttgt gttgtcgctg aatttgcacc tgctgttatg
     6481 tttatcattg ctaatcgctc tgctttaagc gttggtactt tgtcaacttt agttgattgt
     6541 attttttctg ctttgaccga ttgcgtcgtt actgtaattg cgcctgttgt taaaagccct
     6601 agtgctaaac tggttttagc aattgttctc attttcataa ttgtatgctc caatctatat
     6661 tatattcgat tgtcttttta cgtaatttga atcatacaac atcattatag atggcgttct
     6721 aagataatca cattaaaccc cttttaacaa ttattgaagt attattaagt aatttaagca
     6781 aaaaataatg agtgagtatg agattaatat agcgtttcta tgtgcctttg aaataatttt
     6841 taagcattaa aaagaagtta agcaacgttt gatcgtcact taacctctct atttcaattt
     6901 caacttattt cgtcatcaag tatatgtgtt atgcttttat aactttgatt tcaattctat
     6961 caatatctgt gacattgata acatcggaca tacggtcttc ttgtaacttt ttatccaatt
     7021 caaatgtata ctttccatag tatttctttt tgactgtaat ttttcctgta ctcatttcac
     7081 cgtaaagacc ataattatca ataaggtatt ttcttaattt aaaatcaatc tctttcaatg
     7141 acatcgcttc tttatctatt ttaaatggga aaaagtcata atcatattca ccagtatgat
     7201 cttctttaat aactcttgct tctgctatta ggtcgacagc tttatcgttt gcactcgtga
     7261 tacccccaat agagtacttt gcaccttcaa atctcttatc ctcattaacg taaaatatat
     7321 taagattacg atgtacaccc gtatgataat gttgcttatc tttgccaatt aaagcaatat
     7381 tattaacaga attaccatct atgatattca taaatttaat acttggttga atgaaactga
     7441 tataacctgt cacattttta tattcaatac taggttgatt ataataagct tttaattttt
     7501 tgctattttc acttattaca ataggtttct tttcggcatg aactggtttt tccgttgtag
     7561 tgtttacacc tgttgctaat attcctaata acaaacttat ttttgcaata tttttcattt
     7621 tcatagttgt atgctccaat ctattataat tagattgttt tattacgtaa tttgaatcat
     7681 acacccatat tataggagct gtattcggat attcacatta acctgttttt aactattcat
     7741 aaaatatgat taagctattt aagcaaaaga taatgcgtgg tgcgactgtt tgtgtgagtt
     7801 ttgtaacttt tcccctcaaa aaaagaagct aagcaacagt acgtcgctta acttccatat
     7861 atttccataa caagcactat tcaacatagc gctttgttgt tagaaagtat tatttcattt
     7921 ctactagaat tttattaatc ttagtgccgt cgattgactc acccatacgt tctttttcaa
     7981 gtttttgact taaatcgatt gtatgtgttg tgccatcatt cattgtaatt gtaatttgac
     8041 cttgtttaag accatttgaa tacaagccgt gttgtttaat cgcacgttct cttaatctat
     8101 aatcaagttc ttttaatgag atgtcttctt ttgaaatgta gtaaaaatct ttaagtacat
     8161 cttcattatc tttcttgatt tctaaaattg gcgtattgat ataatcaata tactgcacac
     8221 tatttgattt tgtaacgcca ccaattgagt aatttgttgc ttgtctactg tcaccttctc
     8281 taaggataaa cacatctata tttgagtttt ctccgtcttt aaatttatct ttatcagatc
     8341 caagtaaagt gattcgagaa tgctgttgcc ctactgtgac ttctaaatag tgtttaccct
     8401 catcagtata tttaaatcca gtcacatttt tacgctctaa aattggttta ttataatagt
     8461 gttttaactc tgattcactt tgttgtttaa cttctgcttt cgcttgtact gattgtacat
     8521 tcgatgtaat tacacctgtt gctaacattc ccaatgctaa acttgctttt gctatcgctt
     8581 taaatttcat aatttttagc cccaatctat ttaaatttga tttttcaatt acgtaatatg
     8641 aacaatctac ctacattata ttgaggcgct atcccctttc aaattaaacc tcatttaact
     8701 aaacttgaat cattgttaag gtaattaata aaatgctaac tgttattaat caattcttat
     8761 ccgtcgagac tcttttttat acaaatcaca ctttatacca tttttatttt aaaaactgta
     8821 aaactttcag acacaaaaca gacatcatat tgtcgtagta cctgattaaa attttcaatg
     8881 atattagtga tacacatcaa ctaaaacgcg tcattacggc attttcaaca ttaataatat
     8941 ttacgtattt actatacacg tcgacaataa atacagatta gaatttttta taaaatcaaa
     9001 tgttttgaaa a
//
LOCUS       DAKTLH010000109         8889 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.109, whole genome shotgun sequence.
ACCESSION   DAKTLH010000109 DAKTLH010000000
VERSION     DAKTLH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.109"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..122
                     /locus_tag="O0394_002032"
     CDS             <1..122
                     /locus_tag="O0394_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HCY0500102.1"
                     /translation="IYHLYDDRGLWLAFNNNEDYARYSEKYNDLISEFDNEDI"
     gene            complement(215..376)
                     /locus_tag="O0394_002033"
     CDS             complement(215..376)
                     /locus_tag="O0394_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500103.1"
                     /translation="MLLILELRFWNLEFGFRNSTFGIQNYNEWKILIIACLMTWFCSD
                     FYLMNCFFV"
     gene            complement(413..1153)
                     /locus_tag="O0394_002034"
     CDS             complement(413..1153)
                     /locus_tag="O0394_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCY0500104.1"
                     /translation="MFNLKHDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTMPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNVCHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISHFKGDNLIIIN
                     KDRTPYDSDATLVIHDDMVSVVKSLMTE"
     gene            1359..2150
                     /locus_tag="O0394_002035"
     CDS             1359..2150
                     /locus_tag="O0394_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCY0500105.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            2518..2946
                     /gene="lacA"
                     /locus_tag="O0394_002036"
     CDS             2518..2946
                     /gene="lacA"
                     /locus_tag="O0394_002036"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCY0500106.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            2962..3477
                     /gene="lacB"
                     /locus_tag="O0394_002037"
     CDS             2962..3477
                     /gene="lacB"
                     /locus_tag="O0394_002037"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCY0500107.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            3490..4422
                     /gene="lacC"
                     /locus_tag="O0394_002038"
     CDS             3490..4422
                     /gene="lacC"
                     /locus_tag="O0394_002038"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCY0500108.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            4426..5406
                     /gene="lacD"
                     /locus_tag="O0394_002039"
     CDS             4426..5406
                     /gene="lacD"
                     /locus_tag="O0394_002039"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCY0500109.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            5428..5739
                     /locus_tag="O0394_002040"
     CDS             5428..5739
                     /locus_tag="O0394_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCY0500110.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            5745..7457
                     /locus_tag="O0394_002041"
     CDS             5745..7457
                     /locus_tag="O0394_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0500111.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            7475..8887
                     /gene="lacG"
                     /locus_tag="O0394_002042"
     CDS             7475..8887
                     /gene="lacG"
                     /locus_tag="O0394_002042"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCY0500112.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYKGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIIDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
BASE COUNT         3140 a         1339 c         1692 g         2718 t
ORIGIN      
        1 taatatatca tttatatgat gatagaggtc tatggctagc ttttaataat aatgaagatt
       61 atgcaagata ttctgaaaaa tataatgatt taatatcgga atttgataat gaagatatct
      121 aattttttaa tattcgtagc ttttattttt ctacttattt tatgtttatt tttaatctta
      181 caaatgacaa accattaaaa gtagcatccc aacatcaaac aaaaaaacaa ttcatcaaat
      241 aaaaatcgct acaaaaccaa gtcattaaac acgcaataat taaaattttc cactcattat
      301 aattctgaat tccaaatgtc gaattccgaa aaccaaactc caaattccaa aaacgcaact
      361 ccaaaattaa aagcatttcc ctaccattcg ggaaatgctt tttacatact gattactctg
      421 tcattaatga ttttacaacg gaaaccatgt cgtcatgtat gaccaaagta gcgtcgctat
      481 cataaggtgt tcgatcttta ttgataataa ttaaattgtc gcctttaaaa tgtgatatta
      541 atcctgcggc aggttgtaca acgagtgatg aacctagtac aacaagggtg tcagcatgtt
      601 caattttatt taatgccctt atgatggtag gttgatctaa catttcaccg tataatacga
      661 tgtccggtcg aatggcacca ccacaattat cacaatgttt caaagttcta tcaataacat
      721 ctgacttcgt ataagattta tgacatacat tacaataaaa acgatttaac gtgccatgta
      781 attcatcaac atgttgactt ccagcgtctg agtgcaaacc atcgatattt tgcgtgatga
      841 cacctaaaga ttgttgatta cgttctaatt ttgcaatcca atcatgaacg atattgggca
      901 tcgtatcgac aaatagtaag cgcttatggc agaaattgat aaaaccttca ggatcatctt
      961 ctaaataatc acggcttaac aagtattctg gcgaaagccc atcttttgaa atttcatcaa
     1021 ataagccacc cattgaacgg aaatctggaa cgccacttgc gacagataca ccagcacctg
     1081 taaaaaatgt aatacgattc gaactatcta taatatgttt tagtgtctct aaatcatgtt
     1141 tcaagttaaa caccccatca aaataatgaa tgatttataa cgtcttttac ttttattata
     1201 gtatattcat tttacgatta tcaaaagcgc agtttaaaca gttcttttta aaaatgaatt
     1261 atacatataa tcgaacagtt aaattatatt agtttttacg caatttttat tatagaataa
     1321 gcatgtatat taaaattgat gtctaaacat aaaggataat gacccatcgc ctaaataaag
     1381 gaggcaattt tacattgaat aaacatgaac gtttggacga aattgctaaa ctagtgaata
     1441 aaaagggcac gataagaacg aatgaaatcg tcgaaggttt aaatgtgtct gatatgacag
     1501 ttcgaagaga tttaattgaa ttggaaaata aagggatttt aacgaagatt catggtggtg
     1561 cacgcagtaa ttcaacattt cagtataaag aaatatcgca taaagaaaaa catacccgtc
     1621 aaattgcaga aaaacgattt atagctaaaa aagctgcatc attaattgaa gatggggata
     1681 ctttgttttt cggaccagga acaacagtgg aactattagc agaagaagtc aatcatcata
     1741 cgctcacaat tattacgaat tgtttgccgg tgtataaaat tttgttagaa aaacaaacag
     1801 cacattttcg tgtctattta attggtggtg aaatgcgcca tattacagaa gcatttgtag
     1861 gtgaaatggc gaacgccatg ttggaaaaac taagatttag caagatgttc tttagtagta
     1921 acgcagtaaa taaaggtgct gtgatgacat ctacattaga tgaagcctat acgcaacaac
     1981 tggcactaag taattcaatt gaaaaatact tgttaatcga tcatacgaaa gttggcaaag
     2041 aagattttac atcattttgt cagttaaacg aattgactgc agtggtcatg gactatgaag
     2101 atgaagaaaa agtagaaacg attaaaacat atattgaagt agtcgactaa attaaaagta
     2161 taactgcatt gttaaaatat gtatctatat gctcataagt gaatgtgaat ttgcattcat
     2221 ttatgggcta ttttttatgc aaatttatgt caacgggaac ctcgacaaag ttttactaca
     2281 atttcagacg aatacctaat taaaacaaca ccacaatatg aatgttcact tattgtttga
     2341 aaatacgtca taaacaaaca tattataaac atattatgtt gatttattgt ttgtttatgt
     2401 ttataattta aacgtaatca aattgaaagc cttttctcaa gattttcata acctacaaat
     2461 aaatatgtca cattttaaga aaggtatttc aaaattaaag taaaaaggag tcttattatg
     2521 gcgattatta ttggttcaga tgaagctggc aaacgattaa aagaagtcat caaatcatac
     2581 ttattagaca ataaatatga tgttgttgac gtaacagaag gacaggaagt tgactttgtt
     2641 gatgcaactt tggctgtagc aaaagatgtt caaagtcaag aaggtaactt aggtattgtt
     2701 atcgatgcat ttggtgcagg tagctttatg gttgctacaa aaatcaaagg catgattgct
     2761 gctgaagtat cagatgaacg ctctggatat atgactagag gccataacaa ttcaagaatg
     2821 atcactatgg gatctgaaat tgttggagac acattagcga aaaatgtcgt taaaggtttt
     2881 gttgaaggta aatacgatgg tggtagacac caaatccgcg tagatatgct taataaaatg
     2941 tgctaaggag gaaaaattac tatgaagatt gcattaggat gcgaccatat tgttacagat
     3001 acaaaaatgc gtgtatctga atttttaaaa tcaaaaggac atgaagtcat tgacgtagga
     3061 acatacgatt tcacaagaac acattatcca atttttggta aaaaagttgg cgaacaagtt
     3121 gttagcggta atgcagactt aggtgtttgt atttgtggaa caggtgttgg tattaacaat
     3181 gctgtaaata aagtacctgg cgttcgttca gcactagtac gtgatatgac atcagcgtta
     3241 tacgctaaag aagaattaaa tgcgaacgtt attggcttcg gtggacgtat tataggtgag
     3301 ttattaatgt gcgatattat cgatgcattt attaatgctg aatataaacc aactgaagag
     3361 aacaaaaaat taatcgctaa aattaaacat ttagaaacaa gcaatgcaga tcaagctgat
     3421 ccacatttct ttgatgaatt cttagaaaaa tgggacagag gcgaatacca cgattaaggg
     3481 acgacacata tgattttaac tttaacatta aatccatccg ttgatatttc atacccatta
     3541 acagcattga aattagatga tgttaatcgt gttcaagaag taagtaaaac agctggtggt
     3601 aaaggtttaa atgtaacaag agtacttgcg caagtaggtg agccagtcct tgcgagtggt
     3661 tttattggcg gtgaattagg tcaatttatt gctaaaaaat tagatcatgc cgacatcaag
     3721 catgcatttt ataacattaa gggtgaaaca cgaaactgca ttgcaatttt acatgaaggg
     3781 caacaaacag aaattttaga gcagggaccg gagattgata atcaagaggc tgcaggattt
     3841 attaaacatt ttgaacaatt attagaaaaa gttgaagcag ttgctatttc aggtagctta
     3901 cctaaaggat taaatcaaga ttattatgca caaatcattg aacgatgcca aaacaaaggt
     3961 gtgcctgtga tattagattg ctcgggtgca actttgcaaa cagttttgga aaatccatac
     4021 aaacctacag tcattaaacc aaacatttct gaactatatc aattgcttaa ccagccactt
     4081 gatgaaagtt tagaaagttt gaaacaggca gtgtcacaac ccttattcga agggattgag
     4141 tggattattg tgtctttagg tgcacaaggt gcattcgcta agcacaatca tacattctat
     4201 agagtgaata ttccgacaat aagtgtatta aatccggttg gttcaggtga ttctacagtt
     4261 gctggaatta cttcagcaat tcttaatcat gaaaacgacc acgatttact aaaaaaagcc
     4321 aatactttag gcatgctgaa tgcacaagaa gcacaaacag gttatgtcaa tttaaataat
     4381 tatgatgatt tattcaatca aattgaagtt ttagaggtgt aaaaaatgtc aaaatcgaat
     4441 caaaaaatcg catcaattga acaattaagt aacaacgaag gtatcatttc tgcattagca
     4501 tttgaccaac gtggtgcttt aaaacgcatg atggcaaaac atcaaactga agaaccaact
     4561 gtagctcaaa ttgaacaatt aaaagtatta gttgcggaag aattaacaca atatgcatct
     4621 tcaattttat tagatcctga atatggttta ccagcttcag atgcacgtaa caaagattgt
     4681 ggattattac ttgcttatga aaaaactgga tatgacgtaa acgctaaagg tcgtttacct
     4741 gactgtttag tagagtggtc tgcaaaacgt ttaaaagaac aaggcgcaaa tgctgttaaa
     4801 ttcttacttt actatgatgt tgatgatgct gaagaaatta acattcaaaa gaaagcatac
     4861 attgaaagaa tcggttcaga atgtgttgca gaagatattc cattcttttt agaagtatta
     4921 acatacgatg acaacattcc tgataacggt agtgttgaat tcgcaaaagt taagcctaga
     4981 aaggttaacg aagcaatgaa attattctca gaaccacgct ttaacgttga tgtattgaaa
     5041 gttgaagtac cagtaaacat gaaatatgta gaaggctttg ctgaaggtga agtggtttat
     5101 acaaaagagg aagcggcaca acactttaaa gatcaagatg cagcaactca tttaccatac
     5161 atttacttaa gtgctggtgt ttctgcggaa ttattccaag aaactttaaa atttgctcac
     5221 gaagctggtg ctaaattcaa cggggtactt tgtggccgtg ctacatggtc tggtgctgtt
     5281 caagtataca ttgaacaagg tgaagatgct gctcgtgaat ggttacgtac aactggtttc
     5341 aaaaatattg atgacttaaa taaagtatta aaagatactg cgacatcttg gaaacaaaga
     5401 aaataagttt aaaaggggat taatgctatg aatagagaag aagtccaatt attaggtttt
     5461 gaaattgttg catttgcagg ggatgcacgt tctaagtttt tagaagcatt aacagcagct
     5521 caagctggag attttgcaaa agcagatgca ctgattgaag aaggaaacaa ttgcattgcg
     5581 gaagcgcata gagcacaaac aagcctgttg gctaaagaag cgcaaggtga tgatattgca
     5641 tacagtgtaa cgatgatgca tggtcaagat catttaatga caacaatttt actgaaagat
     5701 ttaatgaagc atttattaga attttataaa agagggtgat gacaatgatg caaaaattga
     5761 tcgcacaaat tgaaaaaggg aagccatttt tcgagaaatt atcacgtaac atatatttaa
     5821 gagccatccg tgatggattt atttctgcaa tgcctgtcat cttattctca agtatctttc
     5881 tattaattgc atatgtgcca aatatatttg gttttaaatg ggataaaggc atggaagcca
     5941 ttttaatgaa accatacaac tatacgatgg ggcttgtagc atttttagta gcaggaacga
     6001 cagcgaaatc tttaacagat tcatttaacc gtaagctaga aagtacaaat caaattaact
     6061 ttatttctac aatgctagca gcgatgtgtg gattcttgtt cttagcttct gatccagcca
     6121 aagatggtgg attcttaagt gcatttatgg gaactaaagg tttattaaca gcattcttat
     6181 cagcatttgt aactgttatt gtttataact tctgtgtgaa gcgcaatatt acaattaaaa
     6241 tgccgaaaga agtaccgccg aatatttcac aagtatttaa ggacttaatt ccattttcag
     6301 cggtaatcat cattctttat gcattagatt tagtcattcg caacagcttt aaatcaaatg
     6361 tagcggaagg tattttaaaa ttattcgaac cattatttac agcagcagat ggatggattg
     6421 gtgtcacaat tatctttggt gcctttgcat tattctggtt tgtaggtatt catggtccgt
     6481 caattgtaga gccagcaatt gcagccatta catatgcgaa tatcgaagcg aacttcaagt
     6541 tgcttcaagc tggagaacac gcagataaaa ttattacatc aggtacacaa atgtttatcg
     6601 ttacatttgg aggtacgggt gcaacattgg tcgtcccgtt catgtttatg tggatgacga
     6661 aatctaaacg aaacaaagcg attggacgtg catcagttgt accaacattc tttggtgtaa
     6721 acgaaccaat tttatttggt gcaccactag tattgaatcc tgtgttcttt attccatttg
     6781 tattagcacc aattgttaac gtatggattt tcaaactttt cgttgaagtg ttaggaatga
     6841 atagttttag tgtgaattta ccttggacaa caccaggtcc attgggcatt atcatgggta
     6901 caggttttgg tttatggtca ttcgtactag ctattacttt gattgttgta gatattatta
     6961 tttactaccc attcctaaaa gtttatgata gtgaaattct tgatgaagaa gaaggacgta
     7021 aagaaagtaa ttcagattta aaagaaaaag ttgcagcaaa ctttgatacg aaaaaagctg
     7081 attcaatttt agcggcaagt ggtgtatcag acgatgcagc taaagcttca aatatcacag
     7141 aacaaacaaa tgtcttagta ctttgtgccg gtggtggtac aagtggatta ttagccaatg
     7201 cacttaataa agcagctgaa gaatatcatg tgccagtgaa agcggcagct ggcggatatg
     7261 gcgcacatat ggacattatg aaagagtacc aattaatcat tttagcgcca caagttgcgt
     7321 ctaactatga agatattaag caagatacag atagattagg tattaaatta gcgaagactc
     7381 aaggtgctga atatatcaaa ttgacacgag atggccaagc agcccttgat ttcgttcaac
     7441 aacaatttga aaattaagaa taggagtttt tcatatgact aaaacattac ctgaagattt
     7501 tatttttggt ggagcaacag cagcatatca agcagaaggt gcgacaaata cggatggtaa
     7561 aggtcgcgta gcatgggata cgtatttaga agaaaactat tggtacactg cagaaccagc
     7621 gagtgatttt tataacagat atccagtgga tttagaatta agtgaaaaat tcggtgttaa
     7681 tggtatccga atttcaattg catggtctcg tatcttccca aatggatatg gcgaagttaa
     7741 ccctaaaggt gttgagtatt accataagtt atttgcagag tgtcataaac gtcatgttga
     7801 accatttgta acattgcatc attttgatac accagaagtg ttacacaaag atggcgattt
     7861 tctaaatcgt aaaacgattg actactttgt agattatgct gaatattgtt ttaaagaatt
     7921 cccagaagta aaatattgga ctacattcaa tgaaattgga cctattggtg atggtcaata
     7981 tttagtaggt aaattccctc caggtattaa atatgatttt gaaaaagtat tccaatcaca
     8041 tcataacatg atggtggctc atgctagagc agtaaaatta tttaaagatg gcggatacaa
     8101 aggagaaatt ggtgttgtac acgcattgcc aacaaaatat ccgttcgatc catcaaatcc
     8161 tgaagatgtt cgtgcagcag aattagaaga tatcatccat aataaattca tcttagatgc
     8221 aacatattta ggtaagtatt ctcgtgaaac gatggaaggt gtgcaacata tcttatctgt
     8281 taatggcggt aaattaaaca ttacagatga agattatgcc attttagatg cagccaaaga
     8341 tttaaacgac ttcttaggta tcaattacta catgagtgat tggatgagag gttacgatgg
     8401 agaatctgaa atcacgcata atgcgacagg tgataaaggc ggttctaaat accaacttaa
     8461 aggtgtcgga caacgtgaat ttgatgtcga cgtacctcgc acagactggg actggatgat
     8521 ctatcctcaa ggtttatatg atcaaatcat gcgtgttgtt aaagattatc ctaactatca
     8581 taagatttat atcactgaaa atggtttagg atataaagat gaatttattg aatctgaaaa
     8641 aacagttcat gatgatgcac gtattgatta tgtaagacaa catttgaacg ttattgcaga
     8701 tgctattata gatggtgcaa atgttaaagg ttactttatt tggtcattaa tggatgtgtt
     8761 ctcttggtca aatggttatg aaaaacgata tggtttattc tatgtcgatt ttgagactca
     8821 agaacgttat cctaagaaaa gtgcttattg gtacaaagag ttagccgaaa caaaagaaat
     8881 taaataatt
//
LOCUS       DAKTLH010000110         8814 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.110, whole genome shotgun sequence.
ACCESSION   DAKTLH010000110 DAKTLH010000000
VERSION     DAKTLH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8814
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.110"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            46..723
                     /locus_tag="O0394_002043"
     CDS             46..723
                     /locus_tag="O0394_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
                     /protein_id="HCY0500113.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKASTLEVRSQATQDLS
                     EYYNRPFFEYTNQSGYKEEGKVTFTPNYQLIDVTLTGNEKQNFGEDISNVDIFVVREN
                     SDRSGNTASIGGITKTNGSNYIDKVKDVNLIITKNIDSVTSTSTSSTYTINKEEISLK
                     ELDFKLRKHLIDKHNLYKTEPKDSKIRITMKDGGFYTFELNKKLQTHRMGDVIDGRNI
                     EKIEVNL"
     gene            745..2253
                     /locus_tag="O0394_002044"
     CDS             745..2253
                     /locus_tag="O0394_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HCY0500114.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASENQNALISNIN
                     VDNQEKQNNVNQAVQPQNNTNETSKVPANFVKLNDIKPGDTSIQGTTLPNQFILLTID
                     KKDVSSVEDSDSSFVMSDKDGNFKYDLNGRKIVHNQEIEVSSSDPYLGDDEEDEEVEE
                     TSTEEVGAEEESTEAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKG
                     HTSVKGKVALSINNKFINFETNANGGPNKEEAKSGSEGIWMPIDDKGYFNFDFKTKRF
                     DDLELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLK
                     DVKEDLHVDEIYGSLYHTEKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEGQEFPD
                     LQVDEKGEFSFDVDHAGFRLQNGETLNFTVVDPITGELLSGNFVSKNIDIYESPEEKA
                     DREFDERMENTPAYHKLHGDKIVGYDTNGFPITWFYPLGEKKVERKAPKLEK"
     gene            complement(2356..2664)
                     /gene="spn"
                     /locus_tag="O0394_002045"
     CDS             complement(2356..2664)
                     /gene="spn"
                     /locus_tag="O0394_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HCY0500115.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
     gene            3032..3805
                     /gene="lpl1"
                     /locus_tag="O0394_002046"
     CDS             3032..3805
                     /gene="lpl1"
                     /locus_tag="O0394_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl1"
                     /protein_id="HCY0500116.1"
                     /translation="MGYLKRFALYISVMILIFAIAGCGKGNETKEDSKEEQIKKSFAI
                     TLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWTIYTDFAKSNKPGELDDEGMVLNLDR
                     NTRTAKGHYFVTTFYRNGKLPDEKNYKIEMKNNKIILLDEVKDDKLKQKIENFKFFGQ
                     YANLKELRKYNNGDVSINENVPSYDVEYKMSNKDEIVKELRSRYNISTEKSPILKMHI
                     DGDLKGSSVGYRKLEIDFSKRENSKLSVIEFLSYKPAKK"
     gene            3836..4636
                     /gene="lpl2"
                     /locus_tag="O0394_002047"
     CDS             3836..4636
                     /gene="lpl2"
                     /locus_tag="O0394_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl2"
                     /protein_id="HCY0500117.1"
                     /translation="MRYLKKLAWFISVIILGIFIIGCDSSSDTGEKAKEDSKEEQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWMIYTDFAKSNKPGVLDNEGMIL
                     NLDRNTRTAKGYYFVDTIYDNHENSYSKNYRVEMKNNKIILLDKVEDQKLKERIENFK
                     FFGQYADFKSLKSYNNGDVSINSNVPSYDAKFKMSNKDENVKQLRSRYNIPTEKAPIL
                     KMHIDGDLKGSSVGYKKLEIDFSKEENSELSVVDSLNFQPAKKNKDDE"
     gene            4655..5462
                     /locus_tag="O0394_002048"
                     /pseudo
     CDS             4655..5462
                     /locus_tag="O0394_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            5511..6311
                     /gene="lpl4"
                     /locus_tag="O0394_002049"
     CDS             5511..6311
                     /gene="lpl4"
                     /locus_tag="O0394_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl4"
                     /protein_id="HCY0500118.1"
                     /translation="MGYLKRLVLYIVIMVMSVFIIGCDKSSDTAEKSKEDSKETQIKK
                     SFAKTLDMYSIKNLEELYDKEGYRDGEFEKGDKGMWTIYTDFAKSNKPGELSNEGMVL
                     YLDRNTRTAKGYYFVRTFYRKDKLPDRKNYKVEMKNNKIILLDKVEDKKLKQKIENFK
                     FFSQYANLKELKNYSNGDVSINENVPSYDVKYKMSNKDENVKQLRSRYNIPTDKSPVL
                     KMHIDGNLKGSSVGDRKLEIDFSKRENSHLSVIDSLDYQPAKTNKDDE"
     gene            6330..7100
                     /gene="lpl5"
                     /locus_tag="O0394_002050"
     CDS             6330..7100
                     /gene="lpl5"
                     /locus_tag="O0394_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl5"
                     /protein_id="HCY0500119.1"
                     /translation="MKSIKRIGLCISLLILSIFVTSCDGDNKITGDSKETQIKKSFSK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKGNKSDELDDEGMVLNLDR
                     NTRTAKGYYFVKKFYEKDKLPDRKNYKVEMKNNKIILLDKVEDPNLKKRIENFKFFGQ
                     YANFKDLENYNNGDVSINWNVPSYDVEYKMSNKDENVKQLRSRYNIPTDKAPMLKMHI
                     DGDLKGSSVGYKRLEIDFSKEGRDISVIDYLSYKPAKK"
     gene            7132..7923
                     /gene="lpl6"
                     /locus_tag="O0394_002051"
     CDS             7132..7923
                     /gene="lpl6"
                     /locus_tag="O0394_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl6"
                     /protein_id="HCY0500120.1"
                     /translation="MEYLKRLALLISVIILTIFIMGCDSQSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLEDFYGKEGYRDGEFKKDDKGTWLIRSEIVKQPKGKVMKTRGMQL
                     YINRNTETAKGFFVLKEISENNNRVNKDKEEKYEVKMVGNKIIPTEQINDEKIKKEIE
                     NFKFFVQYGNFKNFEKYNNGEFSYNPEAPIYSAKYQLHNDDYNVRQLRKRYDISTKET
                     PKLLLKGGGDLKNSSVGQNDIEFTFVERKGENIYFNDSVEFIPSK"
     gene            7993..8769
                     /gene="lpl7"
                     /locus_tag="O0394_002052"
     CDS             7993..8769
                     /gene="lpl7"
                     /locus_tag="O0394_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl7"
                     /protein_id="HCY0500121.1"
                     /translation="MKCFQKLYIFILILIVLMAGCESNKITGDSKETQIKKSFAKTLD
                     VYPTKNLEDFYDKEGYRDGEFKKGDKGKWVIRSEMTTELKNENMVSKGMVIRLNRNSR
                     TCTGEYFVRIVKEDSEGKVYSDERKYPVKMENNKIITLKPIDDEKVKKEIEEFKFFVQ
                     YGNFKELENYKDGEVTYNPEAPIYSAQYQLKNSDYNVEQLRKRYNITTKKAPKLLLKG
                     SGNLKGSSVGYKNIEFTFVENKEENIYFTDSINFNPSEDK"
BASE COUNT         3676 a          970 c         1643 g         2525 t
ORIGIN      
        1 gtaatagaat aaatcaaata gactaaaaat tgggagcata gaattatgaa attaaaaaat
       61 attgctaaag caagtttagc actagggatt ttaacaacag ggatgattac aactactgct
      121 cagccagtaa aagcaagtac attagaggtt agatcacaag ctactcaaga cttgagtgaa
      181 tattataata gaccgttctt tgagtataca aatcagtcag gatataaaga ggaaggaaaa
      241 gtgacgttta ctcctaatta tcaacttata gatgtaactt taactgggaa tgaaaagcaa
      301 aattttggtg aagatatttc taatgtagat atatttgttg taagagaaaa ttctgataga
      361 tctggtaata cagcttcaat tggtggtatt actaaaacaa acggttcaaa ttatattgat
      421 aaagtaaaag atgtaaattt aataattact aaaaacatcg atagtgttac atcaacgtca
      481 acatcatcta catatacaat taataaagaa gaaatttcat taaaagaact tgattttaaa
      541 ttaagaaagc atttaattga taaacataac ctttataaga cagaacctaa agacagtaaa
      601 attcgaatta ctatgaaaga tggtgggttc tacacatttg aattgaataa aaagttacaa
      661 acacaccgta tgggtgatgt tattgatggc agaaatatag aaaaaattga agtgaattta
      721 taaaattatt cgagggagca tatcatgagg gaaaatttta agttacgtaa aatgaaagtc
      781 ggtttagtat ctgttgcaat tacaatgtta tatattatga caaacggaca agcagaagca
      841 tctgaaaatc aaaacgcttt aatctctaat ataaatgtag acaatcagga aaaacagaat
      901 aatgtaaatc aagctgttca gcctcaaaat aatactaatg aaacatcaaa agtaccggct
      961 aattttgtca aattgaatga tattaaacca ggtgatactt ctatacaagg aacaacttta
     1021 ccaaatcaat ttatactatt aactattgat aaaaaagatg tgagctcagt tgaagattct
     1081 gacagcagct ttgttatgtc tgataaagat gggaatttta agtatgactt aaatggtcgc
     1141 aaaattgttc ataatcaaga aattgaagtg tcttcatcag atccctattt aggtgacgat
     1201 gaagaagatg aagaagtaga agaaacttca actgaagaag ttggtgctga ggaagaaagt
     1261 acagaagcta aagctacata tacaacaccg cgatatgaaa aagcgtatga aataccgaaa
     1321 gaacagctaa aagaaaaaga tggacatcac caagttttta tcgaacctat tactgaaggt
     1381 tcaggtatta ttaaaggcca tacctctgta aaaggtaaag ttgctctatc tattaataat
     1441 aaatttatta actttgagac aaatgctaat ggtggtccaa ataaagaaga agcgaaatct
     1501 ggatcagaag gaatctggat gcctattgat gacaaaggat actttaattt tgacttcaaa
     1561 acgaaacgtt tcgatgattt agagttaaag aaaaatgatg agatctcatt aacatttgca
     1621 cctgatgacg aagatgaggc attgaagtca ttaattttca aaactaaggt aacgagttta
     1681 gaagatattg ataaagcaga aactaaatat gaccatacta aagtggaaaa agtaaaagta
     1741 ttgaaagatg ttaaagaaga tttacatgta gatgaaattt acggaagctt atatcataca
     1801 gaaaaaggta aaggtattct tgataaagaa ggtactaaag taattaaagg taagactaaa
     1861 ttcgcaaatg cagttgtgaa ggtagactct gaactaggtg aaggtcaaga attccctgat
     1921 ttgcaagtcg atgaaaaagg tgaattcagc tttgatgtag atcatgctgg atttagatta
     1981 caaaatggag aaacactaaa cttcacagta gttgatccta ttacaggtga attattaagt
     2041 ggaaattttg tttctaaaaa catagatatt tatgaatctc ctgaagaaaa agcagatcgt
     2101 gagtttgatg aaagaatgga aaacacacct gcatatcata aattacatgg tgataaaatt
     2161 gtcggctacg atactaacgg attcccgatt acctggtttt atccattagg tgaaaagaaa
     2221 gttgaacgta aggcaccaaa attagaaaaa taattaaata aaacagctta atgatgtaat
     2281 gaaattagtg agttaatcac tgacttctac gtcattgagc tgttttttgt gctttgttac
     2341 aaagcattat tgaatttatt ttacgtgttc atattttgaa acatcaaagc cgtcttgttt
     2401 agctttgttg ataatgtctt tgattgaatg tagtccttta tcggcgaagt atgatcttaa
     2461 gttgtctttt gtagcttggt cagcattctt atctaataac acatcaatat aacttaattc
     2521 atgttctaag aagtttgcat catcatgtag tacgagtcca ttttgagaat aaactttcgc
     2581 atctgcttga ttaccatatc caacaacgcc agttgctaaa acacctacca ttgccgtagc
     2641 tactaaaacc tttttaaatt tcatatctat cactcctcta aaaattgtaa ctccatcata
     2701 acactgaata ttaagaaaat tacgtttatt aagtcgattt aataattttt aataaatagt
     2761 taaagtgaca aatattgttt aaatgcaatt aatctttaat acgatgcttg aggatttttc
     2821 ctaataaaac cttgatttca aaaagggttt aaatcaaatg aaacaataat aaaaaaatga
     2881 cgcaatataa taataagtac aaatttaatt aagaaattaa attgattgta tatgtatatt
     2941 ttggtaacgt aaaagagaaa tatacaaaat aattaattat ttatatgaaa agagaatata
     3001 aatgaagtat aaaacagaga gacgtgaagc gatgggatat ttaaaaaggt ttgcattgta
     3061 cataagcgtt atgattttaa tatttgcgat agcaggttgt ggcaaaggta atgaaacaaa
     3121 agaagattca aaggaagaac aaatcaaaaa gagctttgcg ataacattag atatgtatcc
     3181 aattaagaat ctcgaggact tatatgacaa agaaggatat cgagatagcg aatttaaaaa
     3241 aggtgacaaa gggatgtgga cgatatatac agatttcgcc aaaagtaata aaccgggtga
     3301 attggatgat gaaggtatgg ttttaaatct ggataggaat actagaaccg ctaaaggcca
     3361 ttattttgtt actacatttt accggaatgg taaactacca gatgaaaaga attataaaat
     3421 tgaaatgaaa aataataaga ttattttatt agatgaagta aaagatgata agctcaagca
     3481 gaaaatagaa aactttaaat ttttcggtca atatgcaaat cttaaagaat tgagaaaata
     3541 taataatggt gatgtttcaa ttaatgaaaa tgttcctagt tatgatgttg aatacaaaat
     3601 gagcaataaa gatgaaatag ttaaggaatt aagaagtcgt tataatattt cgactgaaaa
     3661 atcacctata ttaaaaatgc atattgatgg ggacctgaaa ggcagttctg taggttatag
     3721 aaagttagaa attgactttt caaaacgtga aaacagcaaa ttatcagtca ttgaattttt
     3781 aagttataaa ccagcgaaaa aatagtattg ataaggaata ttagggtgtg aaaaaatgcg
     3841 atatctaaaa aagcttgcat ggttcataag tgttattatt ttgggcattt ttataatagg
     3901 ttgtgatagt tcaagcgata ctggggaaaa agcaaaagaa gattcaaagg aagaacaaat
     3961 caaaaagagc tttgcgaaaa cattagatat gtatccaatt aagaatctcg aggacttata
     4021 tgacaaagaa ggatatcgag atagcgaatt taaaaaaggt gacaaaggga tgtggatgat
     4081 atatacagat ttcgccaaaa gtaataaacc gggtgtatta gataatgaag gtatgatttt
     4141 aaatttggat agaaatacac gtacggccaa gggatattat tttgtagata ctatatatga
     4201 caatcatgaa aactcttata gtaaaaatta tagagttgag atgaaaaaca ataaaattat
     4261 tttattagac aaggtggaag atcaaaaact taaagaaaga atagaaaact ttaaattttt
     4321 cggacaatat gccgatttca agagtttgaa aagttacaac aatggcgacg tttcaattaa
     4381 tagtaatgtt ccaagttatg acgcgaaatt taaaatgagt aataaagatg aaaatgttaa
     4441 gcaattaaga agtcgttata acattcctac tgaaaaagct ccaatattaa aaatgcatat
     4501 tgatggggac ttaaaaggca gttccgttgg atataaaaag ttagaaatag acttttcaaa
     4561 agaagaaaat agcgaattat cagtagtcga ttcattaaat tttcagcctg ccaaaaaaaa
     4621 taaagatgat gaatgatagg gaggatgtga aacaatgtgg tcaataaaaa gaattggaat
     4681 gtacacaaat gttataattt tgagcgtttt tataataggt tgtgatagtt caagcaataa
     4741 tgcagaaaat caaagagaag actcaaaaga agaacaaatc aaaaagagct ttgcgaaaac
     4801 gttagatatg taccctatta agaatctcga ggatttatat gacaaagaag gatatcgtga
     4861 tggtgagttt gaaaaaggcg ataaggggac gtgggtattg tattcagcta tggtatcaca
     4921 accaaaaggc gagagtttaa aatcacgagg aatgatatta aagttagata gaaataagag
     4981 aactgctaaa ggaagttata ttattagaga attgaaagaa gataaaaatc atgatgttca
     5041 aaaaaatgaa aagaaatatc cagtgaaatt ggtgaataat aggatagttt tgataaaaga
     5101 tgttaaagac aaaaagttaa aaaaatgaaa tagagtcgtt tgaattattt tcacaatatg
     5161 gaaactttaa tcattttgat cggaatgaga ttactaatat ttcatataat cctaatgctc
     5221 ccaattactc tgcagaatat aaaatgaaga aaaatgacag aaacattcaa cagttgaaaa
     5281 agagatttaa tctaaaaact agcaagacac caaaattatt gtttaaggga tctggagata
     5341 taaaggggtc ttctgtagga tataaggaaa tagaaatcat atttagtaga agtaaagaag
     5401 aaagcattta ttatgttgac agcgttgagt tcgttccaag taacaaaata acaaaaagtt
     5461 gatattagtc aacaaaagca aatattgaga ataaagagag ttgtgaaatg atgggttatt
     5521 taaaaagact tgtattgtat atagttatta tggttatgag tgtttttata ataggttgtg
     5581 ataaatcaag cgatactgca gaaaaatcaa aagaagactc gaaagaaaca caaatcaaaa
     5641 agagttttgc gaaaacgtta gatatgtatt caattaagaa tctcgaggaa ctatatgaca
     5701 aagaaggcta tcgagatggc gaatttgaaa aaggtgacaa agggatgtgg acgatatata
     5761 cagatttcgc caaaagtaat aaaccgggtg aactaagtaa tgaaggcatg gtcttgtact
     5821 tagatagaaa cacacgcaca gctaaggggt actactttgt caggacgttt tatcgcaagg
     5881 ataaattacc tgatagaaaa aattataaag ttgaaatgaa aaataataaa atcattttat
     5941 tagataaagt agaagataaa aagcttaaac aaaaaataga aaattttaaa tttttcagtc
     6001 aatatgcaaa tcttaaagaa ttgaaaaact acagcaatgg tgatgtctca attaatgaga
     6061 atgttccaag ttatgacgtg aaatataaaa tgagtaataa agatgaaaat gttaagcaat
     6121 taagaagtcg ttataatatt cctactgata aatcaccggt attaaaaatg catattgatg
     6181 gtaatttgaa aggaagttcc gttggagata ggaagttaga aattgatttt tcgaaacgtg
     6241 aaaatagcca tttatcagta atagattctt tggattacca gccagcgaaa actaataaag
     6301 atgatgaata atgaggatgg tgtgtaacaa tgaagtctat aaaaaggatt ggattgtgca
     6361 taagtctgtt gattttaagc atttttgtaa catcttgcga tggtgataat aagatcactg
     6421 gagattcaaa agaaacacaa atcaaaaaga gcttttcgaa aacgttagat atgtatccaa
     6481 ttaagaatct cgaggactta tatgataaag aaggataccg tgatggcgaa tttaaaaagg
     6541 gcgacaaagg aatgtggact atatatacag attttgctaa aggcaataaa tcagacgaat
     6601 tggatgatga aggtatggtt ttaaatctgg atagaaatac tcgaacggct aagggatatt
     6661 attttgttaa gaaattttat gaaaaggata aattacctga tagaaaaaat tataaagttg
     6721 aaatgaaaaa taataaaatt atcttattag acaaggtaga agatccaaat ctaaaaaaga
     6781 gaatagaaaa ctttaaattt ttcggacaat atgcaaattt taaggatttg gaaaattaca
     6841 acaatggcga cgtgtcaata aattggaatg ttccaagtta tgacgtggaa tataaaatga
     6901 gcaataaaga tgaaaatgtt aaacaattaa gaagtcgtta taacattcct actgataaag
     6961 ctccaatgtt aaaaatgcat attgacgggg acttaaaagg tagttctgtt ggatataaaa
     7021 ggttagaaat agatttttca aaagaaggta gggatatttc agtcattgat tatttaagtt
     7081 ataagccagc gaaaaaatag tactgataat taaatattag ggtgtgaagg aatggaatat
     7141 ctaaaaaggc ttgcattgtt aataagtgtt attattttga ccatttttat aatgggttgt
     7201 gatagtcaaa gcgatactgc agaaaatcca aaagaaggtt caaaagaagc acaaattaaa
     7261 aagagttttt cgaaaacgtt agatatgtat ccaattaaga atctcgagga tttttatggc
     7321 aaagaaggat atcgagatgg cgaatttaaa aaagatgata aaggtacttg gctaattaga
     7381 tccgaaatag ttaaacagcc aaagggcaaa gtgatgaaaa caagaggtat gcaattatat
     7441 attaatagaa ataccgaaac agccaaaggt ttctttgttt tgaaagaaat aagtgaaaat
     7501 aataatcgtg taaataaaga taaggaggaa aaatacgaag tgaaaatggt aggaaataaa
     7561 attattccta ctgaacaaat taatgacgag aaaataaaaa aagaaattga aaacttcaag
     7621 ttttttgtgc aatatggaaa ctttaaaaat ttcgaaaaat acaacaatgg tgagttttca
     7681 tataatcctg aagcaccaat ttattcggct aaatatcaat tacacaacga tgattacaat
     7741 gtaaggcaac tacgtaaaag atatgacatt tcaacaaaag aaacaccgaa gttacttttg
     7801 aaaggtggag gagatttaaa aaattcctca gttggtcaaa acgatattga atttactttt
     7861 gttgaaagaa aaggtgagaa tatttatttt aacgatagtg ttgaattcat accaagtaag
     7921 taaagttaaa ttaaaatgtg tggttagatg aaatgatcaa atatcaaaaa atgtgcaaag
     7981 agatgtgaca agatgaagtg ttttcagaaa ttatacatat ttatattaat tttaatcgta
     8041 ttaatggcag gatgcgaaag taataagatc actggagatt cgaaagaaac acagatcaaa
     8101 aagagctttg cgaaaacatt agatgtatac cctacgaaaa atctagaaga tttttatgac
     8161 aaagaaggat atcgagatgg cgaatttaaa aagggtgaca aagggaagtg ggttattaga
     8221 tctgaaatga caacagaact gaaaaatgaa aatatggtat ctaaaggtat ggtcatacgt
     8281 ttaaatagaa atagtagaac atgcactggt gaatattttg tcaggatagt taaagaagac
     8341 agtgagggca aggtatatag tgatgaacga aaatatccag tgaaaatgga aaataataaa
     8401 atcattacat taaaaccaat cgatgatgaa aaagtaaaaa aagaaattga agaatttaaa
     8461 ttcttcgtac aatacgggaa tttcaaagaa ttggaaaact ataaagacgg agaagtgaca
     8521 tataacccag aagcaccaat atactctgca caatatcaat tgaaaaatag tgattataat
     8581 gtagaacaac tacgtaagcg atataatata acgacgaaaa aagcgcctaa attattattg
     8641 aagggttcag gtaatttaaa aggctcatca gttggatata aaaatattga atttaccttt
     8701 gttgaaaata aggaagaaaa tatttacttc acagatagta ttaatttcaa cccaagtgag
     8761 gataaataag tatatttagt attacaaaaa cgttagaaaa attaataata aagt
//
LOCUS       DAKTLH010000111         8648 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.111, whole genome shotgun sequence.
ACCESSION   DAKTLH010000111 DAKTLH010000000
VERSION     DAKTLH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8648
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.111"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            873..1067
                     /locus_tag="O0394_002053"
     CDS             873..1067
                     /locus_tag="O0394_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCY0500122.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(1300..2151)
                     /locus_tag="O0394_002054"
     CDS             complement(1300..2151)
                     /locus_tag="O0394_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCY0500123.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTSLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
     gene            2479..3240
                     /gene="sufC"
                     /locus_tag="O0394_002055"
     CDS             2479..3240
                     /gene="sufC"
                     /locus_tag="O0394_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCY0500124.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
     gene            3338..4645
                     /gene="sufD"
                     /locus_tag="O0394_002056"
     CDS             3338..4645
                     /gene="sufD"
                     /locus_tag="O0394_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCY0500125.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            4751..6001
                     /locus_tag="O0394_002057"
     CDS             4751..6001
                     /locus_tag="O0394_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCY0500126.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDVDQLINALKQTKEFFSYEF"
     gene            5991..6455
                     /locus_tag="O0394_002058"
     CDS             5991..6455
                     /locus_tag="O0394_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCY0500127.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            6606..8003
                     /gene="sufB"
                     /locus_tag="O0394_002059"
     CDS             6606..8003
                     /gene="sufB"
                     /locus_tag="O0394_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCY0500128.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            8190..8327
                     /locus_tag="O0394_002060"
     CDS             8190..8327
                     /locus_tag="O0394_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCY0500129.1"
                     /translation="MMVDKATTQIQHDCGIRVLVFIKLIESYIKYSLDNSILVRLRFV
                     V"
     gene            complement(8333..8647)
                     /locus_tag="O0394_002061"
     CDS             complement(8333..8647)
                     /locus_tag="O0394_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500130.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSEDVFKTYGPLATSVVNLILLIVF
                     LF"
BASE COUNT         3137 a         1240 c         1597 g         2674 t
ORIGIN      
        1 ttagacacag caatgtgttc aaattttcat ctattcgtaa gttagccttc gggctgactt
       61 tttatttcca ttattcacat gttaatcttg ttgttattta ggcaggtact tcggtacttg
      121 tctatttttt tatgttataa ttacatgtgt atatagtagg agtgaactat atagcccggc
      181 agaggccata tatctggctg ttggtctcac aggagacatc ttccttgtca tcactcatat
      241 acatatatct tgataacata gagttgttat agtcgctacg ccactcatac tagttactgg
      301 gtggttgttt ttatgttata ttataaatga tcaaaccaca ccacctatta atttaggagt
      361 gtggttgttt tcaagggcaa aaaaagggca taattttgaa acaaagggca agggtatgaa
      421 taataaatta tcttgaaaat tttataaatg catatataac aatgttttga ttgcccttga
      481 acttccttga taatctggct aattattcct gctaaataaa tcatattaat ggctcagtaa
      541 cgtgatgttg ctgggctttt taatttaaac aggtatttat atgatattta ggaatgagat
      601 gctattacgg ataaagtaaa atcctatatg catgcgtgct actcatttat tcttattttt
      661 aatttcacat ggctagtgac taatacatca taaatcatta gtgattcttt attatttcag
      721 tcccactccc tcaagttaat gatgaatcta tttcgctagt tataaaatga cgtcaaatca
      781 atatcaaaaa ttatttaagt aaaatgttta gataattttt cagtgggtaa gtattatata
      841 taacatttaa ttattccgag gaggcattta ttatggcaga cgaaagtaaa tttgaacaag
      901 caaaaggtaa tgttaaagaa acagtaggta atgttactga taataaaaat ttagaaaacg
      961 aaggtaaaga agataaagct tctggtaaag cgaaagaatt cgttgaaaat gcaaaagaaa
     1021 aagcaactga ttttattgat aaagtaaaag gtaacaaagg cgagtaattt tactttttat
     1081 tcctaataca ctgcacatgc cacttagtcg gtataaccat cggctaggtg gtttttgtat
     1141 taaaaaagtg gataccaaaa tttattaata attattttaa tgttagaaaa aaactaaata
     1201 aaaactcgct aatgatatcc aataatatgt atacaaaacg agacatatat tgcatatgat
     1261 taacgagata ctgaaaatat tttatcaccc ctaaaatgat tattcatttt cagcggtaat
     1321 tcgacctaaa gtcaaactta caataaaacc gatgataaat actactaatg aaacgaacca
     1381 catcacgata ttagttggta aacctggaaa tactgcaaag agggagccaa caacaaaacc
     1441 aatgattaat gcaaaagtca ttagtttatg atgtgttagg aaatactgga taattttgct
     1501 tgaaataatg aatccagcaa gcacgccaaa tccgactgca agtaatatag gaagacctgc
     1561 aaagttaagt ttaacaactt cagatattgc tagcatgacc gtaccataga cgccaaatac
     1621 taataacata aatgaccctg aaatacctgg gagtaacata gcactagatg cacacatacc
     1681 tgcaataaaa tatttaataa taagactagt tgatagagta agtgtttctc cagcatgttt
     1741 atcaccatta ttcattaatg taataacaat taagatagcg ataccagcta taaccatcat
     1801 gtaatgttta gttgtaaatg acgttttata gttagaaatt ttcaataaat atggaacgat
     1861 accaatgatt aatccaccaa agaaaaacat agttggaata tggtgttggc ttaataaata
     1921 attaaaaaga ttacttagtg atcccattgc cagtaacatt ccaattataa tggggattaa
     1981 aaatgtaaaa cttggccaaa aacgtcgtga gaatatgccg ctaattgaag cgataaattg
     2041 attgtaaata cctaacaata atgcgatagt cccaccgcta acaccaggta ccaagtcact
     2101 cgttcccata gcaaaacctt ttagaatatt aatccattta aattgttgca tgaataactc
     2161 ctttcaaacg attggaataa aatcataaat agcatcatac catattacaa atgtcctagt
     2221 gaaatgataa catattttaa attcataaaa tccattgaga aattatgtgc acttattatc
     2281 atttatattt ttaaagagag cggattagag ttgtacttta gagttattaa aaataataaa
     2341 aagggtttaa gttgtttata ttaaggtata agtaagttat aattaactga acgcattatt
     2401 acaaagtctt tttgactaca aattaaaatt attataaact agttaagaaa actttatatt
     2461 ttacggaggg aatataaaat ggcatcaaca ttagaaatca aagacctaca tgtgtctatt
     2521 gaggataaag aaatcttaaa aggtgttaac ttgacaatta acactgatga aatacatgcg
     2581 attatgggac caaacgggac aggtaaatca actttatcat ctgcaattat gggacaccca
     2641 agctatgaag taactaaagg agaagtactt ttagacggtg taaatatttt agaattagaa
     2701 gttgatgaaa gagcaaaagc aggattattc ttggcaatgc aatatccatc agaaattaca
     2761 ggtgttacaa atgctgattt catgcgttca gcaatcaatg cgaaacgtga agaaggacaa
     2821 gaaatcaact taatgcaatt tattaagaaa ttagataaaa acatggattt tctagacata
     2881 gataaagaca tggcacaacg ttatttaaat gaaggtttct caggtggaga gaagaaacgt
     2941 aacgaaatct tacaattaat gatgttagaa cctaagtttg caatcttaga tgaaatcgat
     3001 tcagggttag acatcgatgc attaaaagtt gtatctaaag gtattaacca aatgcgtggg
     3061 gaaaactttg gtgcattaat gattacacac tatcaacgat tattaaatta cattactcct
     3121 gataaagtac atgtaatgta tgctggtaaa gtcgttaaat ctggtggtcc agaattagca
     3181 aaacgtcttg aagaagaagg atatgaatgg gttaaagaag agttcggttc agctgaataa
     3241 tcttattaat acagtatcca tgagatgttc atctatatat gatgaaaatg aacatttata
     3301 cgaaatagta aatttcatca agtaggagga aaaagttatg acaactgata ttttgaacat
     3361 ttctgaagaa caacttgttg attattctaa agcccacaat gaaccttctt ggatgacaga
     3421 attacgtaaa aaagctttga aattaacaga aactttagaa atgccaaaac ctgataaaac
     3481 aaaattaaga aaatgggatt ttgattcttt taaacaacac gatgtaaaag gtgatgttta
     3541 tcaatcttta tcacaattac ctgagtcagt aagagaaatt attgacgtag atcattctaa
     3601 aaacttagta attcaacata ataatacgat tgcgtacaca caagttgatg ataatgcatc
     3661 gaaagatggc gttatcgttg aaggtttagc agacgctctt atgaaccata gtgatttagt
     3721 acaaaagtac tttatgaaag atgcagtaac agtagatgaa catcgtatca cagcgctaca
     3781 cacggcatta gttaatggtg gcgtatttgt ttatgttcct aaaaatgtag ttgtagaaca
     3841 tccagtacaa tacgttgtgt tgcacgacga cgaaaatgca agcttttata accatgttat
     3901 catcgttact gaagaaagcg ccgaagtcac atatgttgaa aattacttat caaatgcatc
     3961 tggtgaagga aatcaattaa atattatttc tgaagtgatt gctggtgcaa attcaaatat
     4021 cacatatggc tcagtggact atatggataa aggctttaca ggtcatatca ttcgacgtgg
     4081 tattactgaa gcggatgcct caattaattg ggcactaggt ttaatgaatg agggtagcca
     4141 aattattgat aatacaacaa atttatttgg tgatcgttca acaagttcac ttaaatcagt
     4201 agttgtaggt acaggcgaac aaaaaattaa tctaacatct aaaatcgtac aatatggtaa
     4261 agaaacagat ggttatatcc ttaaacatgg tgttatgaaa gaacatgcat cgtctgtatt
     4321 taatggtatc ggctacatta agcatggtgg aactaaatca attgctaatc aggaatcacg
     4381 tgtattaatg ttatctgaac atgctcgtgg tgacgcgaat cctattttat taattgatga
     4441 agatgatgta caagctggtc atgctgcatc agtaggtcgt gttgatccag atcaacttta
     4501 ctatttaatg agtcgtggta tttctcaaag agaagcggaa cgtcttgtta tacatggttt
     4561 cttagatcca gtagtacgtg aattacctat cgaagacgtt aaacgtcaat tgagagaagt
     4621 aattgaacgc aaagtttcta aataatattt tgaaaataaa agtttgtaat agatatagac
     4681 tgtcgatatt ggtataagac taatacaacg tcagtattta aatgattagg atttttattt
     4741 aagaaaggtc gtgaatgaag tggccgaaca ctcatttgac gttaatgaag taatcaagga
     4801 ttttccgata ttagatcaaa aagtcaatgg caaacgttta gcatatcttg attcaacagc
     4861 gacaagtcaa acgcctgtgc aagtgttaaa tgttttagaa gattactaca agcgttataa
     4921 ttcaaacgtt catcgtggtg ttcatacatt aggatcattg gcaactgatg gttatgaaaa
     4981 tgcccgtgaa accgttcgtc gttttattaa tgcgaagtat tttgaagaaa tcatttttac
     5041 acgcggaaca actgcgtcga ttaaccttgt agcacatagc tatggtgatg caaatgttga
     5101 agagggcgat gaaattgttg tcactgaaat ggaacatcat gccaatattg ttccttggca
     5161 acagttagca aagcgtaaaa atgcgacatt gaaatttata ccaatgacag ctgacggtga
     5221 attaaacatc gaggatatta agcaaacgat taatgataaa acaaagatcg ttgctattgc
     5281 acatatatct aatgtgctcg gtacaattaa tgatgttaaa accattgcag aaatagctca
     5341 tcaacatggt gcaattatca gtgttgatgg ggcgcaagca gcaccacata tgaaacttga
     5401 tatgcaagaa atgaatgctg atttttatag ttttagtggt cataaaatgc ttggaccaac
     5461 aggtattggc gtattatttg gtaaacgtga gttactacaa aaaatggaac cgattgagtt
     5521 cggtggcgac atgattgatt ttgtaagtaa gtatgatgca acatgggctg atttacctac
     5581 taaatttgag gcgggtactc cattaattgc tcaagcaatt gggcttgcag aagctattcg
     5641 ctatttagaa cgcataggtt ttgatgcaat tcataaatat gaacaagaat taacgatata
     5701 tgcttatgag caaatgtctg caattgaagg aattgaaatt tatggcccgc caaaggatcg
     5761 tcgtgcaggt gtaataacgt ttaatttaca agatgtacat ccacacgatg ttgctacagc
     5821 cgtagataca gaaggtgtag cggttagagc tgggcatcat tgtgcgcaac cgttaatgaa
     5881 atggttaaat gtgtcttcaa cagctagagc gagtttttat atatacaaca cgaaagaaga
     5941 cgttgatcag ttaataaatg ccttgaaaca aacgaaggag tttttctctt atgaatttta
     6001 ataatctaga tcaattatat agatctgtca ttatggatca ttataaaaat cctagaaata
     6061 aaggtgtatt agataacggg tctatgacag tagatatgaa taacccgaca tgcggtgacc
     6121 gtatacgact aacatttgat atagaagacg gcattataaa agatgctaag tttgaaggtg
     6181 aaggttgttc gatttcaatg gcaagtgcat cgatgatgac acaagctgtt aaaggtcatt
     6241 cacttggaga agcaatgcaa atgagccaag aatttacgaa aatgatgctt ggtgaagact
     6301 atgtgattac agaagaaatg ggagatattg aagcattgca aggtgtatct caattcccag
     6361 ctcgtattaa atgtgccaca ttagcttgga aagcattgga aaaaggtact gttgctaaag
     6421 aaggtaaagc agaaggtacg actgaagaag aatagaatgc tgttaatcat agataatttt
     6481 gatattagac atataaaagt ataaaaattt ttataagatg tcatgtcatt gttataatat
     6541 ggtttacatc atgaattaaa aacttacgca cgccgttgta aatatatttt taaggagtga
     6601 ttgaaatggc taaaaaagca cctgatgttg gggattataa atatggattc cacgacgatg
     6661 atgtgtccat tttcagatca gaacgtggtt taactgagaa tatcgttaga gaaatttcta
     6721 acatgaaaaa tgagccggaa tggatgttag atttccgtct taaatcatta aaattgtttt
     6781 ataaaatgcc aatgcctcaa tggggtggcg acttatcaga attgaatttc gatgacatta
     6841 cttactatgt aaagccttca gaacaagctg aacgttcatg ggatgaagtg ccagaagaaa
     6901 ttaaaagaac tttcgataaa ttaggaattc ctgaagctga acaaaaatat ttagctggtg
     6961 tttctgctca atatgaatct gaagttgttt accataatat ggaaaaagaa cttgaagaaa
     7021 aaggtattat ctttaaagat acagatagtg ctttacaaga aaatgaagaa ttattcaaaa
     7081 aatactttgc ttctgtagta cctgcagcag ataacaaatt tgcggcgtta aactcagcag
     7141 tatggtcagg tggttcgttc atttatgtac ctaaaaatat caaactagat acgccactac
     7201 aagcttattt ccgtattaac tctgagaaca tgggtcaatt tgaacgtaca ttaatcattg
     7261 ctgatgaagg tgcttctgta cattacgtag aaggttgtac tgcaccagtt tatacaacta
     7321 gttctttaca ctctgctgtt gtggaaatca ttgtgcataa agatgcgcac gttcgttata
     7381 ctacgattca aaactgggcg aacaatgtat acaatttagt tacaaaacgt acttttgttt
     7441 atgaaaacgg aaatatggaa tgggtagatg gtaacttagg ttctaagtta acgatgaaat
     7501 atccaaactg tgttctttta ggtgaaggtg caaaaggtag tacattatct attgcatttg
     7561 ctggtaaagg acaagttcaa gatgccggtg ctaaaatgat tcataaagca cctaatacat
     7621 cttctacaat tgtttctaaa tctatttcta aaaatggtgg taaagttatt tatcgcggta
     7681 ttgttcattt tggacgtaaa gcaaaaggtg ctcgttcaaa tattgaatgt gatacattaa
     7741 tcttagataa cgaatcaaca tcagatacaa ttccatataa cgaagtattc aacgatcaaa
     7801 tatcattaga acatgaagcc aaggtttcaa aagtttctga agaacaatta ttctatctaa
     7861 tgagtcgtgg tatttctgaa gaagaagcga cagaaatgat tgttatggga ttcatcgaac
     7921 catttacaaa agaacttcca atggaatacg cggtcgaaat gaaccgttta atcaagttcg
     7981 aaatggaagg tagtattgga tagctttaaa ccgcgttgtt aagccattct tgacttccga
     8041 aaatggctat tgataccatt ttgatactga atataacaaa aagccacatt actgtggctt
     8101 tttttgtttt ataactaaat cggattgata gataagcttt gtacttattt atatcagttc
     8161 gattttttga ttggtgtaaa aaataatcat tgatggtgga taaagcgaca acacaaatac
     8221 aacatgattg tggcattaga gtgctggtct ttattaaatt aattgaaagc tacatcaaat
     8281 attctttaga taattcgata ttagttcgat taagattcgt tgtataagtg agttaaaata
     8341 agaaaactat taataatatt aagttcacta cagatgttgc taatggacca taagttttaa
     8401 agacatcttc acttttataa ccaacaatcg catctaaaaa ttgaactaag atcattgcaa
     8461 tggatatagt tatcaaaaat atagcactat gaatgactaa agaaaaaata gctaataaaa
     8521 ataaaggtaa gcttcgacta agtgcataat atgcatttat attatggcta gatgcacatg
     8581 cttgaattga ataacctaaa cttacactgg cactgattat tgtaaatatt gctaaaacaa
     8641 aatacatg
//
LOCUS       DAKTLH010000112         8554 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.112, whole genome shotgun sequence.
ACCESSION   DAKTLH010000112 DAKTLH010000000
VERSION     DAKTLH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8554
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.112"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(191..1549)
                     /locus_tag="O0394_002062"
     CDS             complement(191..1549)
                     /locus_tag="O0394_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HCY0500131.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            complement(1666..3219)
                     /gene="gntK"
                     /locus_tag="O0394_002063"
     CDS             complement(1666..3219)
                     /gene="gntK"
                     /locus_tag="O0394_002063"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCY0500132.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYVMRESKVNKDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVEALELLGISKEMLPELVPTTYVMKGM
                     KERYATLMGLNKDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPIEENVTVYQEIVSIFINLSRSLTENYEQIADFQR
                     QHIAENKTQ"
     gene            complement(3243..3923)
                     /locus_tag="O0394_002064"
     CDS             complement(3243..3923)
                     /locus_tag="O0394_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0500133.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            complement(4077..4841)
                     /locus_tag="O0394_002065"
     CDS             complement(4077..4841)
                     /locus_tag="O0394_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0500134.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIISA
                     ILMKNKWPFLIALPFMIGYGIGVTFYYQQKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN"
     gene            complement(5006..5698)
                     /locus_tag="O0394_002066"
     CDS             complement(5006..5698)
                     /locus_tag="O0394_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCY0500135.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(5929..6345)
                     /locus_tag="O0394_002067"
     CDS             complement(5929..6345)
                     /locus_tag="O0394_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCY0500136.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKTEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            complement(6484..8307)
                     /locus_tag="O0394_002068"
     CDS             complement(6484..8307)
                     /locus_tag="O0394_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCY0500137.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKSAFLQVAKTAVDSFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSEENVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPNVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLAIFENIAK
                     HFELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
BASE COUNT         2656 a         1685 c         1253 g         2960 t
ORIGIN      
        1 tttcataatt atcgcctgta caatacgcac aaaaacatgt cttgaaacgc ctttcatcac
       61 tctaaaatac ccaatatact ttttatatcg ttcggattct gagtatttca gacgattttc
      121 tgcataaaaa taaacgtgtt tcaaggcaat atattgcaat tacctaaaaa cacgtttact
      181 taatatttag ttaaacaaat aagctaatga ataaaatgaa gatgatacct gaaacggaaa
      241 taatcgtttc taataatgac catgttaaga atgtttcttt tacagttaaa ccaaaatatt
      301 ctttaaacat ccaaaatcct gcgtcattta catgagacaa aatcacacta cctgcaccta
      361 tcgcaagtac aactaatgca acatttacat ctgatgattg taataatggt aagacaatac
      421 ctgtagttga aatcgcagct actgtagccg aacctaatgc gatacgtagc acagctgcaa
      481 caatccatgc tagtaaaatc ggagacatct ctgtaccttc aaacatttta gcaattgtat
      541 ttccgacacc gccgtcaatt aatacttgtt taaatgtacc gccaccgcca ataatcaata
      601 acatcattcc gattggataa atcgcattcg tcactgattc cataatatga ttcatcttac
      661 gctttctcat taatcccatc gtaacgattg caaataatac tgctattagc atggctgtcc
      721 ctgctgttcc tatcatataa atgatagatt caaatagatt tgtaggtttg tcatgcccag
      781 ttacaagttg cgttatcgta gacactaaca ttaatatgac tggtaatgtt gctgttaata
      841 aactcatacc aaatcctggc atctcttgat ccgtaaattc tttttgtgca cctaacgctg
      901 aaatatcgcc ttctcgtgta tacgcagacg gaatcatttt ttgtgcaact ttgttaaata
      961 taggccctgc aatgagtgta actggaatgg caataatcat accatacagt aatacatctc
     1021 caacatttgc ctttaattct tttgcgatga ctaccggtcc tggatgtggt ggtaaaaagc
     1081 catgtgtcac tgataaagct gttaccatag gtagtcctag ttttaacact gaaacatttg
     1141 cgcgttttgc tactgtaaat actaatggaa tcagtaagac taaacctact tcaaagaaca
     1201 atgcaatacc gacgataaat gctgcaacaa gcattgccca ttgtacatgt ttttgaccaa
     1261 atttttgaat caacgtgtct gcgattcgag ttgcaccacc accatcagca agcaatttcc
     1321 caagtatggc acctaaaccg aatatcagtg caatgtggcc gagcgtactg cccattcctt
     1381 tctcaatcgt ctccataatt ttagtcaatg gtatacctag cattaacgct gtaatcatcg
     1441 atgtgataat taatgaaata aatgtattta atttaaaccc aataattaat actaataaaa
     1501 taacgatacc taaaacaaca ctgattaacg gccatatttc gttaaacatg acattcccct
     1561 ctttctcttt tcaatagaat gtaacaccgt cgtcgcgcac gtattccaca taatatatag
     1621 tagtcatggc acaacaactt tatctaagat gcttgagtga cgtatttatt gtgttttatt
     1681 ttcagcgata tgttggcgtt gaaaatctgc aatttgttca taattctctg ttaaagaacg
     1741 acttaaattg ataaaaatgg atacgatctc ttggtaaaca gtgacatttt cttcaatcgg
     1801 cgtatgattg tttgtggcac cgaccatcga tgaaacgatt gaaaaatctt caatgtcacc
     1861 tacagcttta agtccgagca cgcaggcacc taagcatgaa ctttcataac tttcaggaac
     1921 cactaactct gtgtcaaata tatctgacat catttgacgc catacttcac ttttcgcaaa
     1981 accacctgtt gcttttatca tcttaggtgt ttcattcatt acttcaataa gcgcaagata
     2041 gacggtatac aaattgtaaa gaacaccttc taatgcagcg cgaatcatat gttctttttt
     2101 atgagataaa gttaaaccga agaatgaacc tcttgcattt gcgttccaaa gcggcgcacg
     2161 ttctcctgct aaatagggat ggaatattaa accatctgca cctggtttaa cacgctttgc
     2221 aatttgagtt aagacatcat aaggatcaac accgagacgt ttcgcagttt cgacttcact
     2281 cgctagcaac tcgtcgcgca accatctcaa tacgacacca ccattattta caggacctcc
     2341 gatgacgtag tggtcctctg ttaagacata acaaaatatt ctacctttgt aatcagtacg
     2401 cggtttatct atcacagtac gaatcgcccc agatgtaccg attgtgacag caacttctcc
     2461 tttaccaaca ctattgacac ctaaattaga aaggacccca tcactcgcac caataacaaa
     2521 cggtgtatct ttattaagcc ccattaatgt tgcataacgt tctttcatac ctttcatcac
     2581 atacgttgtt ggaactaatt ccggcaacat ttccttggaa atacccagca gttctaatgc
     2641 ctcaacatcc caatctaatg tttctaaatt aaacatccct gttgcggaag ccattgaata
     2701 atcaatgata tatgtatcaa ataaatgata gaaaatgtat gttttaatat ctgcaaactt
     2761 agcagtacgt tgaaatacat cttgccattc atgtttcatc caaaaaatct tcgctaatgg
     2821 cgacatagga tgaatcggtg tgcctgttcg ctggtaaatc gcattgccat catgcacttc
     2881 atttattact gttgcatatt ttgcagcgcg gttatctgcc caagtaatat tatttgttaa
     2941 tctttgatgt tgctgatcca tcgcaatcaa gctatgcatt tgcgcactaa atgacacaaa
     3001 cttaatgtcg tctttattaa ctttggattc tctcataaca tatttaatag tcattagtac
     3061 tgcatcaaat aattcatctg ggttttcttc tgagacatca acgtttggtg tgtgtaaatc
     3121 atagcctatt tgatgtttca tgataaaagt tccattttca tcatataaga ctgacttggt
     3181 actcgtcgtt ccaatgtcga caccaatcat atatttcatg ataaatcctt ctttctttca
     3241 ttttaattca accaaaatcc ttcaatatct ttaccaacat cgtcgaaatt taaatgaaac
     3301 gcttctttca aaatttgact gtcgtattgt tccactgcat caataaacac ttgatgatta
     3361 tgatgtatgc gttcaaaatc ttgcgggttc tgttgcatac gttgtcgcat ggatgttaaa
     3421 acgagtgcca tcattactgg ttttaaatga ctccaaaatg agtttaaata ttgatgatta
     3481 gatgctttga ttaatgtttc atgaaattca aagtcatgct tcgtaaatga ttctgcatcc
     3541 tcaaatttta ctgccacttt catcatttca agttgtttct tcatttcttt tacgatgggt
     3601 agtcgctctt gatttttaac tcttgaaaat gcaaatgact ctaacatcag tcgcaaatca
     3661 tacatttctt tcttttcttg ttccccaaac ggcaacacat gtgcacccat tctttctaat
     3721 tggatgagtt gattttgttg caataattta aatgcatctc gaattggcga acgactcaca
     3781 ttaaattgct ttgccatttg attttcagtg agtaacgtac cttcagctat gtgaccattc
     3841 acaatgccta agcgtaattc tgccgcgata ccttctccag ttgtcatacc ttccaaccat
     3901 ttctctggat atccatacat catcaaagtc actccttcat tacacgacat acttgtatac
     3961 aagtatgtta atatagttat tatgagtttg caagcgcttt ctttacgagc actaaaatag
     4021 tgaccacccc ttttcgattt aaatttaaag gaaatggtca ctatcacacg aatgatttaa
     4081 ttgttatgtt gtatgtggga tatttctaat tgttctgtac tcatatgcgc tttaggtact
     4141 tcaatgcaat aatgcgtttc atgacagttt ggacattcga atcgacgtgt tgtcgctgta
     4201 tgtttcgctt tgataactgc ccacaaagat ggtgagaata tatgctggca gttaggacat
     4261 aaataggcaa ccttttgttg gtaataaaaa gtaacaccaa tgccataacc aatcataaat
     4321 ggtaaagcaa ttaaaaacgg ccatttattt ttcatcaaaa ttgcacttat aatgctagaa
     4381 tattgaatta ttcctataat accagcacta atccaaatgt tacgacgaat acttttcatt
     4441 tcagctgatt tactcatgac atgctctatg tcttttaagt gtgtgattgg agacgtcgac
     4501 gcttcattta cgtaatattg aacattttta attttgttta ataccgcttg ttgctgttta
     4561 acttgttggt taatttcttg ttgtttcata gttagtaaag tattgagcgt cttcaaagta
     4621 ccttcacctt ttagcaacat atctatatcg cttaacgcac aacctaaatc tttaagcaat
     4681 aagattaact ctaatgtttg tcgctgttgt tctgtataca cacgacgctt tccttctgta
     4741 aatccttgtg gtttcaaaat acctttgcga tcataatatt gaatcgttcg tgttgtcaca
     4801 ttgcataatt ttgcgagttc tccagtcgaa tagttagaca tagattccac ctcctataat
     4861 taccatagtt gatgacccga cgtcacgagc aagtacaatt tccacatttt aaagaaattt
     4921 attatactag gcgtcttatt tttatgattt cgtaccatgt tgatttacaa actcactcaa
     4981 actaagtaac acacctacta aacatctact ctgttatttc agaatgaatt tgttgtaatt
     5041 tatcttcaac ttcagtaatc tctgtcgcac attctttcag taaatctcga tacttttccg
     5101 tctctgcatt gtttttataa cgtattttat gttctaaact tgcccacata tccataccta
     5161 tcgttctaat ttgaatttca acaggcaata cctcaacgcg ttctgctaaa aagactggaa
     5221 tagatacaac gatatgtaaa ctgcggtaac cattttcttt agggtgctga atataatctt
     5281 tacgttttat caattgtacg tcttcttgtt taagcaacat ctcttcaatc aaataaatat
     5341 catctaagta attacatact acgcgaattc cggcaatatc cagtatatgt tctttggcag
     5401 tttctgcgct aatctgtaat cctttacgat taagcttttc tattaaacta cgcatttctt
     5461 tcacacgtcg ctccatatga tgtataggat tgtgtttata aatgtgattg aaattgtcat
     5521 ctaaaatact cagtttagtg cttatttcct ttagtgctga cgaataaata tgatctaact
     5581 ctacgaaacc taataacgta tcaaatgctt catcaccatt ttcaaattta cttaagctat
     5641 ttttaaaatc atgtcgcaaa tcctctaaat atagtgatgg ttttcgatct acatacattt
     5701 ttatactaac ctccgatata taatcatctt tattgtacct aacattttat taagataata
     5761 ctaatacact atcgaacttt gggctgatac tgaaacagca caaagaaact caaacaattc
     5821 gaattatgta tcaaaacctt caattacagg aagtgatttt aaaatgattt aacgcaaaaa
     5881 aacacctgtt accgttatat aggtacaagt gcttaattga tagagatgtt atacgtcttt
     5941 aaatgattcc acaacttttg gatgtggacc atccataagc ggttctcttt gtcgaacgcc
     6001 accactttgg ttgccaattg attcgctatc aaagtacgat ttatcttgat ttgattgttc
     6061 ttgaatatgt tcttcattat cagttgttgc atattcacta tccacctctg ttttttccat
     6121 tgttgctgta tggaattgta cgaagttctc ttcttctaat gctttgattt cttctttcga
     6181 taacgctcga taccaatctt tcgctttttt cgccgcaata ggaacaacat ctttttcaag
     6241 atatccttct tttaacattg cattcgcaat ttctatcgct ttttgccgtt taattacttc
     6301 aaattctttc catttttcgg ggtaaccttt cattgtaaag ggcatttcct taacctccaa
     6361 tatgttataa ttcatgttat ataccaccat aattataaaa tgaaacatat taaacacatt
     6421 tttttagact cccgtcataa taacttcaga caaacgttag tctgacctga taaatattta
     6481 attttaaacg ttcttgtctc tctcacgaaa accattgtgt tgaatattca tcattgctga
     6541 ttgaatataa ttatactgtg gtaaatctgg taatatcttt atttctaatt ctgtatttaa
     6601 ttcgaaatgc ttagcgatgt tttcaaatat tgctaaatac tcgcccatta attgttcatt
     6661 tattgtaagt ctatcttcat tagccatggc tctatttaac ataaagctaa tttcttctaa
     6721 tgcgaataag ctaggataat aattttgaat caatgtctta tcactaaata attcaccatt
     6781 agctgcatta taaacttgcg tcatgttatt taatttcaca gataggttca tagccgtgtt
     6841 tttctgatac acatcatctt tatattgatt ttcagaaaat aaataatgaa agagcgttgc
     6901 ttctttacga acaacttcag ccattacatt aggaagcaat gcggatgcgg tacgttgtcc
     6961 aaggacaaat aaacctatga ttgcaatcac aatacccatt gcaacgtcaa taactcttgg
     7021 aaacgcaatg ttaattgtta aattctgtga tgctaatccg ttcattaaaa taacttgtat
     7081 tgtaataaaa actactgcaa tcgcataatt tgctcccacc aatgcttcag taaacaatgc
     7141 tgcaatgccc attaaaatta ctgcaacagg tgttggtatt gaaaacaaca atatgactga
     7201 aagtactaac acacctaaaa tagtacctaa acctcgtgcc atacctctct cgattgcatg
     7261 tatagttgat gttcctagta atattgtatg tgcagataac ggtatccagt atgctttttc
     7321 aaaatcaaac attaacgcaa taaatatcgc tatcatcata atcgctgtat atctcaatgt
     7381 atttctaaaa acaatagagt ctaatgttaa attttgataa atgcgtttac tatataaagg
     7441 ttttcgcatg tctgcttcat acgcaatacg cgcttcattt gcatgcacca tttcatcaat
     7501 tttcaatata tggttgaata aattttgaaa ttcctctgtt actgtcactt cttttcgcca
     7561 atatcttaca ttttcctcac tattatctag ttgtgcaatg atatgatcca tcatttcaat
     7621 taactcatct ggcaatggtc gaatttgttt agcgttcaac tctaacagtt cagaataaat
     7681 cccttgggca gatgtgtgta ataataataa tttttggaaa cgtctactta atttatcgtt
     7741 acttgaagta gaagtgatta ataatttatc agatgcttta aaactatcca ccgctgtttt
     7801 tgctacttgt aaaaaagcag atttatcatt ataagtgtgt aacaacttag atatgagttt
     7861 aaaatcattt tgaattgctt gttcttcagc tttgttttta gaaaatacga tcgtgattaa
     7921 caccatcata gtggcaagta taccgcctac taaaatcgca aagcctctat ataatgcttc
     7981 ttcgggagct ataggtaagt taatgggtag actgaatgtc acaatgaaga atgtcgatga
     8041 cggtccagct atttttaagg cagtaaatat ataaaatggt ataaccgtaa caattaataa
     8101 tgtcattcca aaaacgagtg gcgttttggc tgttagcgta ccaagcatca tacatattgc
     8161 aaacgctaaa ttacaaatta ttacagttcg cagcttagat cgcgacggac ctttaaaaac
     8221 ataaatatgg gctagtgttc cggttgcaac taataatcca aattggaaat taccacataa
     8281 gtaaccgatt attgcaggaa taatcattaa cagtccctgt cttacacctt tataaaagtc
     8341 taagttaagt cgattgattt ttatgagtga tgtcaaaaag atgtcatttc gacttctcct
     8401 ttccttgtca aaatatttcc ataatctttt tattagcata gttaaaatga atttgttgct
     8461 ataaatttta attacgcaac atatgtcatt ataacacctt atgctcaatt atctaaattt
     8521 aaaaactttt aaagtccaat taaaattaat ttct
//
LOCUS       DAKTLH010000113         8427 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.113, whole genome shotgun sequence.
ACCESSION   DAKTLH010000113 DAKTLH010000000
VERSION     DAKTLH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8427
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.113"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            75..1598
                     /locus_tag="O0394_002069"
     CDS             75..1598
                     /locus_tag="O0394_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500138.1"
                     /translation="MSKKLKIIIPIIIVLLLIGGIAWGVYAFFANTPKNTYLKSEQQT
                     AKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIK
                     MSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLST
                     YSKLTGEDEETAKENGITNQQLNLNTLFNNAQAQQSDYSKIAEKYSELIVDKLDDDNF
                     DKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKD
                     FEKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLE
                     DDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYNDKYEFGKKTEYDESKIKLD
                     NQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEP
                     INFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            1666..2832
                     /locus_tag="O0394_002070"
     CDS             1666..2832
                     /locus_tag="O0394_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0500139.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLTTVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRTLITWFYTMLWSIVGVVWIVFSIPNIFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            2829..3749
                     /locus_tag="O0394_002071"
     CDS             2829..3749
                     /locus_tag="O0394_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0500140.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            3763..4422
                     /locus_tag="O0394_002072"
     CDS             3763..4422
                     /locus_tag="O0394_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0500141.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWTYTY
                     Y"
     gene            complement(4474..5424)
                     /gene="lytM"
                     /locus_tag="O0394_002073"
     CDS             complement(4474..5424)
                     /gene="lytM"
                     /locus_tag="O0394_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HCY0500142.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(5750..6742)
                     /locus_tag="O0394_002074"
     CDS             complement(5750..6742)
                     /locus_tag="O0394_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCY0500143.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWKSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHTVEVSFKEGE
                     VVIKDNPIGVLTNHPDLNWHYSNLRQYINISPYPATANLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNELVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            6976..8352
                     /locus_tag="O0394_002075"
     CDS             6976..8352
                     /locus_tag="O0394_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0500144.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAS
                     NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSIICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
BASE COUNT         2832 a         1432 c         1358 g         2805 t
ORIGIN      
        1 aatttaaaat aaaccattta ataaaatgta ttgttctgat atgattaact cataaataat
       61 tgggaggaag caaaatgtct aaaaagttaa aaattataat tcctattatt attgtcttac
      121 tattaatagg tggaatcgca tggggagttt atgcattttt tgctaataca ccgaaaaata
      181 catacctaaa aagtgaacaa caaacagcaa aaatgtataa agattatttt aatgaccgtt
      241 ttgaaaatga agtgaagttc caagaaaaga tgaaagataa ttcgttttta tcttcattag
      301 aattaagcgc agatgcatca gatgaaattg ttaaagggct tggcattcct aaatctgttg
      361 ttaatgcatc gaaaattaaa atgtcatatg gacatgatcc taaaaaagag aaatcaatga
      421 ttaatcttga accaacaatc gcagactctg cattagggaa attccagtta gctgcagata
      481 aagataagca ttatttcgaa tcaccattat ttaaagggaa atatagtgtt aataattctg
      541 atttattatc aacttattca aaacttacag gtgaagatga agaaacagca aaagaaaatg
      601 gtattacaaa ccaacaacta aatttaaata ctctttttaa taatgctcaa gcacaacaaa
      661 gtgactacag caaaattgcc gaaaaatatt ccgaacttat tgtcgacaaa ttagatgacg
      721 ataattttga taaaggtaaa aaagaagaaa ttaaggttaa tggtgaaaag tacaaagtta
      781 gacctgtcac gttaacactt agcagagctg acactaaaaa aattacatta gctgtattag
      841 aagaagctaa aaaggataaa gaccttaaaa aattaatgga agaacaaggt gctacaaaag
      901 actttgaaaa agacatcaaa aaagcaattg acgatgtcaa agaaactaaa aaggatgaat
      961 ttgctaaaat tcaatctaaa atttataccg aaaaacatac gattgtaaaa cgagaaatta
     1021 ctattacaga caaagaaaat aataaaacta aaatcaaagg tactaatact ttagaagacg
     1081 ataagttaaa actagattac gcacttgatt tcgatcaaga taaatacacg tatgctgaag
     1141 cgaaatatac aattaaaggc gtatcttcta aagaaaaaga taataaatat aatgataaat
     1201 acgaatttgg taaaaagaca gaatatgatg aatcaaaaat caaattagat aaccaagaaa
     1261 aagtagatgg cacaaaacgt caagataaag gtaaaatcac tgtcgcatta gataaatata
     1321 gcgacgaaaa tgaattcact tttgaaaata atatagattc tgacgtaaaa aataacactc
     1381 agaaatctac gttaaatatc ggcatcaaat atgctgaaga accaattaat ttcattttaa
     1441 aatctagcac aaaattgaaa gcagatattg attttgatga tagtggtgca aaagatttca
     1501 atagtctatc ttcaaaagac cgtgaaaaac ttgaaaaaga aatcgaaaaa aatggcggca
     1561 aaatgtttga atcaatttta aaaaaggcat ctaaataact aaaatataaa aataagattg
     1621 gaaggtcatc ttgcctttca atcttatcat tggaggagtg gttaaatgaa aacaatacat
     1681 ttgtttcgca tctaccactc ttttttattg aaaaagtggt atttgattat ctatttacta
     1741 tttatattag cggcactcct tattacatta acgacaatcc aacatgtaac agaagatgac
     1801 aatcatttta atataggtgt cgtagataaa gatcaatcaa gtgaaacgaa attaatctta
     1861 aactctattg gtaaagggag taacctagga aaaaacgtga gcattaaagc atatgatgat
     1921 aagcaagcac atactttgtt aaaaaaacat aaacttcaag gctattttgt ttttgataaa
     1981 ggtatgacca aggcatttta taaacaaggc gaactaccaa tttcagtata tacatatgat
     2041 caacaatcca tgaaaagtgt cgtgctatct cagctaacag attctgttta ccaacgtctt
     2101 atgcgatcaa tgggtggcat cttagctttt caagacttag caccgaaagc atcacattct
     2161 gacagtatca atgttatgac tgatttgctg attacaggat taaaccgttc aggtgcattt
     2221 aacttagaac cgattcattt atacgatacg ggcagttatt atgcaattac aggattttta
     2281 acaacggtat tcatctttgc actatcttta tttacagttt tgaaaatgaa tcaagatact
     2341 gtattgaaag cgcgattgaa aatgtttcat ttttctaaag agcgtttatt aatcattcgt
     2401 acgttgatta catggtttta tactatgtta tggagtatcg ttggtgtagt ttggattgtg
     2461 ttcagtattc cgaatatctt tgaattatat aattggccaa cgttagccat tcatttaagc
     2521 tattatgtta cctttttaat actgtggtta ttactcatcg aacttttaac aacaggcttg
     2581 ttgaatagta ttagtaaagt catattagct attgtcatcc tggtgttatc tggtttaaca
     2641 atacctacga tttttttaca acatatagca aatggtgttt tcaatattca accgttcgca
     2701 gttgtaacaa atcaattgtt agaaattatt ttaaacaact acattttaga actgcaccct
     2761 agtttctatc ttagcttcat cgcacttttg ataattaatt tagctgtatt ggtttggagg
     2821 tatcgtcaat gaagccttat atacaacttg ttgtgttcaa gcaatggtta caatacatct
     2881 tgctcgtaac aaccattgtc atcgcactcg tacttattgg tatcggttac cgtgtagcac
     2941 atgacaactt caaaataccg attaccattc aagatttaga ccaaaccact gcatcaaaat
     3001 cattcgtcaa taaaattaaa caatctgact atgtaactat taaaaaagtc gatgaagatg
     3061 aaagctatat tgaagatgat gttactaaaa aggaagctat tttaagtatg caaattccta
     3121 aaggtttctc tcaaaaatta aaagagaacc gtttaaaaga aacgatacag ttatatggta
     3181 gagatgactt tataggtggt attgctgtag aaattgttag tagttcatta tatgagcagc
     3241 aaattcctaa cattatttat gaacaccttg aggatatgaa acagcatcaa tccatcgatg
     3301 ctatcaacaa gtcctatcat aaacatacac ctgaatctaa aatcaaattt gtgtcgctta
     3361 ctaaacaagc acaacactct atttcaatta gcttaatctt tgcggtgatt ttatttgtta
     3421 gcgctgttca agtagtactt cattatcgtt taaaccaaca agcagcattg caacgattat
     3481 cacaatatca tttaagccgt ttcaaactat atagtactta tgtaatgaca catacgattt
     3541 tgttattgtt ggtactattg gcagttagtc tatatttgtc tcaaccactc agcttaatat
     3601 tttacttaaa atcactgtta cttatattga tttatgagat aggtatcgtc tttatcttat
     3661 tccatattca aacaataagt catcgattat tcatgacatt tatatatgca cttgctatgg
     3721 gtatcgtata cttgattatt ttcatgtaaa ggagcgtaac tgatgataga aattaataac
     3781 ctttcaaagc gttaccgtaa caaacagatt ttcaatcatt taactatgtc ctttgatagt
     3841 aatcgtttaa ccgtattact tggtgataat ggtgctggaa aatcaacatt acttcgtatg
     3901 attgctggta ttgaaaaagc taatgatgga actatcaact atttcggcga aaaatggaat
     3961 caaagacaaa tacaaaatca catcggttat gtgccacaag acattgcgtt atttgaacac
     4021 atgacagtgg ctgaaaacat taaatttttt aaatcacttt gtaaaaatcc aattaacgat
     4081 acaactatca acgaatattt acagcaatta aactttgatg atacgtctgc caaagtatct
     4141 acattgtccg gtgggaataa acgtaaaatt aatatattag taggtttact aggtcaacct
     4201 cgaattctca ttttagatga accgacagtt ggtattgatt taaaatctag acatgacatc
     4261 caccaactac ttaacatcat gaaatctaaa tgtttaatta tattaactac ccatcattta
     4321 gatgaagttg aagcacttgc agatgatatc aagttaattg gccaagatcc tttttatcaa
     4381 catgttttag aggacaaaca atggacttat acctattatt aaaacgaaaa aatcccaagc
     4441 tgcgtatgat atcgcaactt gggattttct gtattatcta ctttgcaagt atgacgttgg
     4501 gtctactgca tattgattac cgatgccacc agacatacgt tggaagtgta cgtgaggcgc
     4561 tgttgaatta cccgtactac ctgaatatgc aatttggtca ccagctttga ctttatcacc
     4621 agctgaaaca gttaaacgat tattatgcat ataccattgg tagttattac tgttcgcttc
     4681 tttaatcgtt acttgattgc cgccaccata gttactccaa cctgcttgta ctactgtacc
     4741 atcagttaat gagtaaactg gtgaattttc aggcattgca tagtcgacac cgtaatgcgc
     4801 accaccaccg tgatattgtc catatggttg tagttgttta cgacttgtta accagcttgc
     4861 gtctttcgca tgaccactag ctgtcgcttt acttgctgat ccaccatttt gattagatgt
     4921 gccattagga taattgacct ttccattacc atcatggctg ttatacgctt ggttgttgtt
     4981 actatgtgaa taatagctcg cgtctggacc tacatttgat tgataaccat attgattaat
     5041 atgctgttgg ctttgactcg ctgtgtagtc attgttatct cctgctgttg ctggattcac
     5101 atatgtttgg ccgcttccat tggcatttgc attttttgga taacagttat aaaaataatg
     5161 cgtatgtcct tgagcatcta cgaatgtata gctatattct ttattatcaa acattgcttg
     5221 attccagtta ccatcaggtg tgtgatgata atccccatta gaatcaattg tataataagt
     5281 accataagat acgtcttgtg attgtgttga catttgtgta tgtgcttgtt gggtgtttgt
     5341 cgtttctgct gcatctgctt gatgcgccat tgtaaatgta gcgaagccca tcgttgcaat
     5401 cgctgctgct gttaattttt tcatgtataa aacatcctcc attaaagtta aagttagttt
     5461 tcaattaaac tgtactgcac atactaaaag aattagacaa ctgagtaaag gatttaattc
     5521 tcattttcca actatttaat attcccgaaa tgttttacta aactcattac attgtcatta
     5581 caaaatagcc atacattgat attaaaatga catctctcac tgcattcgtt taacctttta
     5641 taaattttca aaattaacaa ctaatcgttc gtccatgttc gcgattcaac gctaatgcat
     5701 gatagtaatc atccatcaaa tcatatcaac caaattccat tatcaatcgc tattgattgt
     5761 cattcaactt tctaatagtg atatgcttct caggcttaaa aatcgtcata tcttttctat
     5821 taattaaatc atctgtgagc tttaatgcta ctaattcatt gctgccataa tacttaatat
     5881 ataacgttct tgtagttaaa tttattacgg tctgatacat cgtatagtga ttgtcagcat
     5941 catgcggacg tacaattcca atcggtatat ttaccgcatc taataaataa aatgcattca
     6001 ttaaatccat ttctttatca ttgttttgag caatgtttgc tttcataaat gccattctca
     6061 caaagcgctc agttgaagta aatccacctg gcaatccaaa tgtacctgct tcattgccta
     6121 aaggttcaat cgttacacct tccaataaat ttgctgttgc tggataagga gaaatattga
     6181 tatattgtct taaattacta taatgccaat ttaagtctgg atgatttgtt aagacaccaa
     6241 taggattatc ttttataacc acttcaccct ctttaaatga aacttcgact gtatgtccag
     6301 ttgcatcgga aacatgataa tgcaatggcg gaacttcacc gatgtcattt aaatatacag
     6361 ctacaacatg tatttgggat gcttgttgtt tcatatcttc aatgcttgtt gtatatccca
     6421 aaatccatgt cacaatttca ttttgcgtaa tattcatcgc gtccgctttg tgtgttgatc
     6481 catatgaact ataacctcgg aaatattgtg ttgaaatggc aacgccatgt tcattaacac
     6541 catcaccata aataaaacct tccatatctg ttcctgtgcc aataaagcca tattgcgttt
     6601 ggcctgtcgt gccagtgcga gatttccaac gataatttct aggcgtcact gctggcgaac
     6661 catctaatgg ataatcataa tccatcgtgc gtccaagaag tacttgatta tttaaagttt
     6721 gtattgtgaa tcctgtgcac attgttctca ctcctctgta ccttcattta ctttaatcac
     6781 tttcaaataa agctgtttca cttaaacata ctataaaaaa tcaattatac aagcaattaa
     6841 ttgatattca ttctcaataa ctgtggtatg atatgtaagg aaatcatgac ttatgtgtga
     6901 gtgaacgatc atctatacat ccgttcactt catctcatga ctttctatat ttaattttta
     6961 caaggagtga catctgtgaa taacacacaa tcttcaccac gcagtaatat tattattgcg
     7021 attatgttgt ctgcattaac atattggttg tttgcacaat catttattaa tataggacct
     7081 ctcgttggtc aaacgtatca aacctctcct gccgtgttaa atttatctat tagtttaact
     7141 tccttcgcca caggtatctt catggtggct gcaggtgata ttgctgataa aataggacaa
     7201 ctgagaatga catacatggg tctcataatc agtatgtttg catctcttct attaattata
     7261 tcggacatca ctgcactgct catcatcggt agaattttac aaggtctatc agcagctatc
     7321 ttgttacctt caacagttgg cgtgttaaat aatcaattta aaggagaaca tttaagacga
     7381 gcgattagtt atctaatgat tagtactgtt ggtggcatcg gcctagctgg tgttatcggc
     7441 ggtttaattg cctcaaattt cggatggcaa acgaatttca tcattagtat agtcattgct
     7501 ttcattgcca tattgcttct aaaaggcaca cctgaaaaag taagtcaaca tagccaccgt
     7561 catccattcg attacaaagg tatgtcgatt ttcgctgtta tgattggtag ctttacatta
     7621 ttgttaacac aaggattcga acaaggttgg tttagtacat tttcaatcat ttgtctgagc
     7681 atttttatca tcactacgtt gatattcatc atcatcgaac gtcgacatga agtacctttt
     7741 attgatttct cagtattacg caaccgtccg ttcattggtg catttttaaa taactttgtt
     7801 ttaaatagcg gtctaggcgt aacagtggtc tttttcatat atgctcaaac acaccttggt
     7861 ttatcagctg cgcaatctgg acttgttaca ttgccatatg ccattgtggc agttgcgatg
     7921 attcgtttag gtgaaaaagc aacattacgt ttcggtggca aattgatgct catcattggt
     7981 ccgttgttcc ctgtcatcgg catcactatt attagcatga ctcaattatt ggcatcacaa
     8041 tatgtcattg cagttatcat tggtttcgtc atatgtgcga taggtaatgg tttagtcgca
     8101 acacctggac ttacgattgc aattttcagt atgcctaatg aaaaagttgg tttagctaca
     8161 ggattatata aaatgagtgg tacattaggt ggcgcctttg gtatagcact aagtactaca
     8221 gttttcagta tgttacaact aaactatgca ccaagtgtag ctgcaaccgt aacatttata
     8281 gtcagcattg tattgatgat ccttggctca ttgtctgcat acatgatcat tccaaaaaca
     8341 gttaaatctt aaatataata gaagaattat gtttcgaaat atctttatca ctttaaaatg
     8401 atatacaaga aatccaagaa aaataag
//
LOCUS       DAKTLH010000114         8299 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.114, whole genome shotgun sequence.
ACCESSION   DAKTLH010000114 DAKTLH010000000
VERSION     DAKTLH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.114"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            210..1286
                     /locus_tag="O0394_002076"
     CDS             210..1286
                     /locus_tag="O0394_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCY0500145.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAIEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
     gene            1534..2195
                     /locus_tag="O0394_002077"
                     /pseudo
     CDS             1534..2195
                     /locus_tag="O0394_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            2188..2964
                     /gene="fetB"
                     /locus_tag="O0394_002078"
     CDS             2188..2964
                     /gene="fetB"
                     /locus_tag="O0394_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCY0500146.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLILCVLVIIINASWNTISRASPVMHHVFWISFLAIFIGTALP
                     LAGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            3360..4547
                     /locus_tag="O0394_002079"
     CDS             3360..4547
                     /locus_tag="O0394_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0500147.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(4609..5961)
                     /locus_tag="O0394_002080"
     CDS             complement(4609..5961)
                     /locus_tag="O0394_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HCY0500148.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLESARNKAQHFQKMMLDYLDTDDVTSLSTNDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(6569..8206)
                     /locus_tag="O0394_002081"
     CDS             complement(6569..8206)
                     /locus_tag="O0394_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCY0500149.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIIVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
BASE COUNT         2709 a         1279 c         1583 g         2728 t
ORIGIN      
        1 gtaacaataa aagaataacg tattataata aggaatttta aattgtgact ttttcggaat
       61 attaaatttt agaaatatga ggtttttaag cggattcctc acaaaatttt aaaaatattt
      121 aagcctgaaa atgataaagc ggtagggaac gtttttctga aagttagtga tacaatagtt
      181 ttaagttgaa atacaggagg atgaataaca tgaatcagtc agtcaaatta cttaaacatt
      241 taacagatgt aaacggcatt gctggttatg aaatgcaagt taaagaagca atgcgtaact
      301 atatagagcc tgtcagtgat caaattattg aagataactt gggtggcatt tttggaaaga
      361 aaaatgctga gaatggtcaa tactcaatta tgatttctgg tcatatggat gaagttggtt
      421 ttatggtaac aaagattgat aaacatggtt ttatttcatt tacgccagtt ggtggatggt
      481 ggaatcaagt catgctatct caaaaagtaa cgattacaac agattcgggc aaagaaatta
      541 gaggtatcat cggttctaaa ccgccacatg tcttaacgcc tgaagaacgt aaaaagccaa
      601 tggaaatcaa aaatatgttt atagatattg gtgttagtag caaggaagaa gctgaagaag
      661 ctggcgttga agtaggcaat atggttacgc catatagtga atttgaagtg cttgcaaatg
      721 ataaatattt aactgcgaaa gcatttgata atcgctatgg ctgtgcatta gctattgagg
      781 tattaaaacg tttaaaagat gaaaatattg gcattaactt atacagtggt gccacagtgc
      841 aagaagaagt tggtttgcgt ggtgcgaaag tggcagcgaa tacgattaaa ccagacttgg
      901 cgatagctgt cgatgtaggt attgcttatg ataccccagg tatgtcaggt caaacgagcg
      961 atagtaaact aggcggtggt ccagttgtca ttatgatgga tgctacaagt attgctcacc
     1021 aaggtttgcg aaagcatatt aaagatgtag ctaaggaaca taacatcgaa gtacaatggg
     1081 atacgacacc aggtggaggt acagatgcgg gaagtattca tgtcgcaaat gaaggtattc
     1141 caacgatgac aatcggtgtt acgctgcgat acatgcattc taatgtttca gtgctcaatg
     1201 tagatgatta tgaaaattct atccgtcttg ttactgaaat tgtccgttca ttgaatgatg
     1261 aaagttataa aaatatcatg tggtaatcaa atccataaat aataaagaat ccttttaata
     1321 tggtaggttg ttaaacaatt gtctaatttt aattcttagt cattagacag tatccatgtt
     1381 aataggattt tttgttttta atttaaatgc tgaaaatcaa ttatgcctaa attttgatat
     1441 tacaagaaaa tgattttttc ttaaatgtaa ttgcactaaa aaccaaaaaa acgggaataa
     1501 tatacctgat atattacatg aggagcggtg caaatgttgt tagaaattaa agatttagtg
     1561 tataaagcga gcgatagaat catactagat atatcagtct aaaagtagat aaaggcgaga
     1621 gtattgccat tataggtcca tcaggtagtg gtaaaagtac atttcaaaag caaatatgta
     1681 atttgtttag tccaactagt ggagaacttt attttaaagg taaaccctat aatgattatg
     1741 acccggaaga attgcgtcaa cgaatcagtt atttgatgca gcaaagtgac ttgtttggtg
     1801 aaacgattga agataacatg atattcccat cacttgcacg taatgataaa tttgatagaa
     1861 aacgtgcaaa gcaattaatt aaagatgtcg gtttgggaca ttatcaatta agttcggaag
     1921 tggaaaatat gtcgggtggt gagcggcaaa gaattgctat agcgcgccaa ctgatgtata
     1981 caccggatat tcttttatta gatgaatcga ccagtgcatt agacgttaat aataaagaaa
     2041 agatagaaaa tatcattttt aaattagcag atcaaggcgt ggcaattatg tggattaccc
     2101 acagcgatga ccaaagtatg cgacactttc aaaagcgtat aacaattgtt gatggtcaaa
     2161 tttctaatac agaggagttg aatcaacatg agtaatacgg cacttggatt gacggcattg
     2221 cttttagtca ttccgattat catttcatat aaagaaggtt tacatattat taaagattta
     2281 attgttgcga cattacgagc agttgtgcaa ttaatcattt tgggattttt gctgcattat
     2341 atttttaaaa taaacgataa atggctgctt attttatgtg tattggtcat tattattaat
     2401 gcatcatgga atacaattag tcgagcatca ccagtgatgc atcatgtgtt ttggatatca
     2461 tttctagcta tcttcattgg aacggcatta ccgcttgcag gtactattgc gacaggggcc
     2521 attcaattta ccgcaaatga agttatacct atcggcggca tgcttgcaaa taatggcttg
     2581 attgcaatta atttagctta ccagaattta gatcgtgcat tcgtacaaga tggtactaat
     2641 attgaatcta aattatcact tgcagctaca cctaaattgg cttctaaagg tgcaatacgt
     2701 gaaagtattc gtttagctat agtgccaact attgattcgg ttaaaacata tgggcttgtg
     2761 tcgattcctg gtatgatgac aggcttaatt attggtggcg taccaccttt acaagcgatt
     2821 aaatttcaat tgttagtcgt gtttattcat acaactgcga ccattatgtc tgctttgatt
     2881 gcgacatatt taagctatgg tcaatttttc aatgcaagac atcaattagt agcacgaaat
     2941 actgatgtta agagtgaatc atgatagatt ttactgcatc agatttaggc attagtttta
     3001 attggaaatg aagtgacgcg cacatatagt atcgctattc attagcgcag cgaaaatatt
     3061 cataaaggca cgcatacttt gtagtcagtt atctgttctg acatataaag cgtgcgtgct
     3121 tttttggagt tattgttgaa actgaagtaa ttatacataa ttattaaatg acatacttgt
     3181 gttaattttt caaatactga aaaacaattt caataatttt ccaattaagc acagaaaatt
     3241 aaagcaaaat attatataat agaacggtta tatataaaat tgtgttgcac acatttttta
     3301 ataaatcgtt attctaaggg aaatgaatat cggaaatttt gtttgaaagg agttttaaat
     3361 tgtcaatcat gcgactattt acattcattt taagtatttt tatcgtagga atggttgaaa
     3421 tgatggttgc aggaattatg aacttgatga gtcaggactt acatgtatca gaagctgtcg
     3481 ttggtcaatt agtgacaatg tacgctttaa catttgcgat atgtggacct attctggtta
     3541 aattaacgaa ccgtttttca tcaaggcctg tattattatg gacattactt atatttatca
     3601 ttggtaatgg cattattgct gtagcgccaa atttttcaat attagtagtt ggtagaatta
     3661 tctcatctgc agcagcagca ctaattatcg taaaagtatt agctattaca gcgatgttat
     3721 cagcacctaa aaatcgtggt aaaatgattg gacttgtcta tacagggttt agtggtgcta
     3781 atgtttttgg tgtaccaatt ggaacggtta tcggcgattt agtaggttgg cgctatacat
     3841 ttctattctt aattattgtg agtattattg ttggcttctt gatgatgatc tatttaccga
     3901 aggatcagga aatacaacga ggccctgtga atcatgagac accatctcat gaaaatcatg
     3961 ttacttcgaa aatattaaga cctgctgaag tagcaaaata tttaattatt acgtttttag
     4021 tattgattgc aaactcagtg acattcgtct ttataaatcc acttatttta tctaatggtc
     4081 atgatatgtc attcgtttca ttagcacttc tagtaaatgg aatcgctggc gttattggaa
     4141 catcattagg tggtatattc tccgataaaa ttacaagtaa gcgttggtta atgatttctg
     4201 tttctatttt tatcgtcatg atgttactta tgaatttaat cttacctggt tcaggtctat
     4261 tgttagcagg actatttatt tggaatatca tgcaatggag tactaatcca gcagtgcaaa
     4321 gcggtgtgat tcaacatgtt gaaggcgaca caagccaagt aatgagttgg aacatgtcta
     4381 gtttaaacgc tggtattggt gttggaggca ttattggagg cttggtcatg acacatgttt
     4441 ctgttcaagc tatcacatat acgagtgcca tcattggcgc attaggatta atcgttgttt
     4501 tcacattgaa aaataatcat tatgctaaaa catttaaatc atcataattc tcatatgaaa
     4561 agcacgcctg ctatcaaatt caggtgtgct tttttagatg cgataacgtt attgatatgt
     4621 gcgataatag cgacgttcat tatgatacat cggccaaggc attttaccgc ttttagcaaa
     4681 attagctaaa tcattttgca tttgtcgact taaaaattta aggtgagcag ttgttggata
     4741 ttgatgtgct gctaatattt gtaagtttcc gaaccaaaat accatatcta gaatatggta
     4801 agcactgcga tagtgtgcgc tagaagtatc atgccatgca aattcagcaa gccatagttg
     4861 tgcgtttgaa tcttgtatat tgagttgttg taaaaattgc ttcatcggct gtttgaaata
     4921 acaatgtgta atcgctaaag attgttgctt cgccgtctga acatcttcgt attttaaagg
     4981 aatatcattt aattccataa tgtcgataaa gcgttgcggc gataattttt tctgttcact
     5041 agtaatataa atatcgcctt catcttttgt ataacatgca aaaattggtt tcgttgttgg
     5101 ataattattt tgtatataat ctgttttaat aggcgcatat attaaatcaa gccctttaga
     5161 aggtcctcga gattgtttta gcttcgccat cagcataaga atatcatttg tcgataatga
     5221 tgtaacatca tctgtatcta aataatcgag catcattttt tggaaatgtt gtgctttatt
     5281 gcgtgcactc tcaagggtgt ctaatcgtag tgcgccactt agtagaacga ctttatggaa
     5341 gtatggctca atgtcaggta ttttaagtaa agtcaaaatg ctcatactgc ctgcagactg
     5401 acccattaaa gtaatgttat tagcgtcgcc accgaaggat tcaataaatt gatgcaccca
     5461 ttttatgaca ttgatttgat ctgaaaggcc attattggaa tgaaaatctt tattaaaata
     5521 tgaccagtct aaatatccta atgcgcctaa acgataattg catgtaataa cgataatgtc
     5581 gttattttgt actaaatgtg ccggttgata gagttcggct gtaccatgac cattttcaaa
     5641 actaccacca taaaaataaa tgatgacagg tttcttcgtc tgatcattat gttgtttcca
     5701 aatatttaga tataaacagt cttcatgttc agtaaaagtt gtagattgtg aggagaaaaa
     5761 atcttctaat ttgttgtctg gttgcggtgg gatgggttgt atttcagttg catcaattgg
     5821 ctctgaccat tgtgttttta acgtagaatg tttaaagcga ttgtcatgaa ctggtggttc
     5881 tgcataagga atgcctaaga agatatctaa accatcttgt gtaataccat gaatttgacc
     5941 acctgtagta ttaatcttca tatttattaa cctcctttat catccccaat ttaatcatac
     6001 cacggtctat agatgatagg tgacaagtat aacaagacaa ttttgtgact gaatcagacg
     6061 agcttgatat ctgttttaac tcaaaccatt ttcagcatta tatgatgtac ttctacggga
     6121 gtgggacaga aatgataaag agccacaaat gatttattat gtagtggttc ttatacatta
     6181 gccacagcta atgtgtactt aaaaatatga atacatgagt aaaactcatg cataagaaat
     6241 actaatttct atagaaaaag tatttcttta tcgtcgtccc accccaactt gcattgcctg
     6301 tagaatttct tttcgaaatt ctctgtgttg gggcccctga ctagaatcga aaaaagcttg
     6361 ttacaagcgc attttcgttc agtcaactac tgccaatata acttcgtaga gcatagaaca
     6421 ttgatttatg tcccagcctg atcatcatct tgttatgtta gttatttata accgattttc
     6481 aaaataaaaa accaaccaag atgttgaaat caatggttgg ttaatgtgtg tgtgttgaaa
     6541 tcatatatgg cactgtattt taaaatattt aatgagatga ctttgattca ttatttagtg
     6601 actcacgcat cttcgtattg attcgtgttg cgcgacggaa atagacgctt tcaaatgaac
     6661 tcgttgtacc gatgtaatag aagataagtg ccacaataat aataacgata aagtcaaatg
     6721 gatagtgaat catatttaag cctttaaatt ctttgcttcc tataaatgac agtattgata
     6781 gcacgattaa atatacaata atccataagc taccaccaat ttgtttcttt gtattacgcc
     6841 aattcatacg atattcatag aagaagtaga ttggtaatcc aagtataatg attaaaataa
     6901 cttcagccgt tgttggccac attgcccaat atatagctaa tgaagctaat acaaatgata
     6961 atggtgccat tacttttaaa atttttgctc taaatggacg agtcattgtt ggtcccattt
     7021 ttcttaatgc aatcactgtc gttgggccag ttaaataggc tactaaagtt gcagtagaaa
     7081 taactgctgc tagcgtaccc caatctctaa ataatgtaac cataatcata ctaatgatgg
     7141 cattaaagat gattgctaca cgtgggatat gatatttttc attcatcttc ccaagaaatt
     7201 tagggatatg tccatttttc tccattgctc gtaaaactcg acctgtaacg gcgacaaatg
     7261 acacgccagt accgaatggt gatacaaaag cttcaatgta tagtaaaatt gcgagccaat
     7321 taattcctaa taagatagct aaatcagcaa atggtgaatt gaagttgatg ccattccatc
     7381 cactatgttg taacattgat tgaggcatag aagtgataaa cgtactttgt aaaatgatgt
     7441 acaacactgc actgattgac agtgagatag cgatgcctct tgcgatattt ttttcaggat
     7501 ttttaatttc tgaacccata ttaataattg tctggaatga attgaatgaa aaaataatcc
     7561 ctgatgctgt tgttgcagca aaaatcggtg cacttccgta aggcataaat gtgcttgccg
     7621 aatggccgta atttgaagtg tcgaatcctg atagcatcaa catgatgatg gttaacatgg
     7681 gtacgcctaa tttaaataca gaaattaaac tcgtaaatga tgttaaaagt tttacggacc
     7741 aatagtttaa taatgaaaaa ataacaatga tgagatatac agctagcaat ccgtatgtgc
     7801 taatagagcc attttccatt aaatatctca ttggtttcgc ccaatgccac ggccaagaac
     7861 tcatatattg aacagctgac acagcttcga taggtattat tgtcaccaaa gacacccaat
     7921 tcgcccaagc agcaataaag cctaataatg agccatgtgt atactgggca tagttactca
     7981 tgccacctga ttgaggaaac attgtgccga tttcaatgta gttataggca atggttccaa
     8041 tgactaggaa tccaagaacc catgatatga ttgctgctgg tccagctatt gatgatgctt
     8101 cccacgcacc aaatagccaa ccagatccta ttaaagagcc taaccctaat aagactaatt
     8161 gggatagatt gattttgtcg ttgtgcaatt gcttgtcttt attcataaat actccttcgt
     8221 tattgccaaa aatacaaaaa ataatatgcg acaattatac gcttattata ataatagtca
     8281 aatgcatttt ttgtaagaa
//
LOCUS       DAKTLH010000115         8119 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.115, whole genome shotgun sequence.
ACCESSION   DAKTLH010000115 DAKTLH010000000
VERSION     DAKTLH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8119
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.115"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..487
                     /gene="sek"
                     /locus_tag="O0394_002082"
     CDS             <1..487
                     /gene="sek"
                     /locus_tag="O0394_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="staphylococcal enterotoxin type K"
                     /protein_id="HCY0500150.1"
                     /translation="GKKLDVFGISYNGQCNNKYIYGGVTATNEYLDKPRNIPINIWIN
                     GNHKTISTNKVSTNKKFVTAQEIDVKLRKYLQEEYNIYGHNGTKKGEEYGHKSKFYSG
                     FNIGKVTFHLNNNDTFSYDLFYTGDDGLPKSFLKIYEDNKTVESEKFHLDVDISYKET
                     I"
     gene            575..1795
                     /locus_tag="O0394_002083"
     CDS             575..1795
                     /locus_tag="O0394_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCY0500151.1"
                     /translation="MWLEKFKNKNKETKYRYYEKYKDPLTAKWRRVSVVLNKNGKQSQ
                     KEAQRLLNERIEEKLNDKKPTTLKSLTFHVACDEWFQNYIKTSGSKRTTIKTKLSKLN
                     TLKKFVDEDILINKITLSYAQQVFDEMDSKGYVYQVNKDALSIFKNVFEYTRRIYKLQ
                     DLEFLKDITLNKRIKSYDEVKAKRNKYLELNEIESIIKDINTKAQKMHSGIHKRFYLF
                     VALMTEFQALNGMRIGEMLAIQNEDIDFDNKSLNINGTIHWFHDESGGFGVKDTTKTE
                     SSYRTIGLSSRSCEILKKAILENKKDSKWNDGYLNRNFVFTNHKGNPMQTERFNKILR
                     EAAKDVGIDKEVSSHILRHSHISLLSQQGVSLKAIMDRVGHSDHRTTLSIYSHVTEQM
                     DKDMMNKLEQVKLG"
     gene            complement(1938..2759)
                     /locus_tag="O0394_002084"
     CDS             complement(1938..2759)
                     /locus_tag="O0394_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0500152.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            complement(2777..3472)
                     /locus_tag="O0394_002085"
     CDS             complement(2777..3472)
                     /locus_tag="O0394_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0500153.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            complement(3465..4490)
                     /locus_tag="O0394_002086"
     CDS             complement(3465..4490)
                     /locus_tag="O0394_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCY0500154.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEQGPVTQVFENPQHTVTKRFVKDDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     regulatory      complement(4579..4682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4740..5036)
                     /locus_tag="O0394_002087"
     CDS             complement(4740..5036)
                     /locus_tag="O0394_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0500155.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFKSVTDLLENLK"
     gene            complement(5029..5415)
                     /locus_tag="O0394_002088"
     CDS             complement(5029..5415)
                     /locus_tag="O0394_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCY0500156.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
     gene            complement(6157..7035)
                     /locus_tag="O0394_002089"
     CDS             complement(6157..7035)
                     /locus_tag="O0394_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HCY0500157.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMQ
                     EETLNQSKIGGMPFLKYFKDIPLDENNVPMVLLAQINLDDLPEQQELFPVKEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQAHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
     gene            complement(7214..7594)
                     /gene="gcvH"
                     /locus_tag="O0394_002090"
     CDS             complement(7214..7594)
                     /gene="gcvH"
                     /locus_tag="O0394_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCY0500158.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVAIIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQIEALLTAEKYSEMIGE"
     gene            complement(7752..8108)
                     /locus_tag="O0394_002091"
     CDS             complement(7752..8108)
                     /locus_tag="O0394_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCY0500159.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
BASE COUNT         2722 a         1439 c         1160 g         2798 t
ORIGIN      
        1 gggaaaaaaa cttgatgttt ttggtattag ttataatggc cagtgtaaca ataaatacat
       61 atatggtgga gtcacagcta ctaacgaata tctagataaa cctagaaata tccctataaa
      121 tatatggatc aatggaaatc acaaaactat ttctaccaat aaagtttcga caaataaaaa
      181 atttgttacc gctcaagaga ttgatgtcaa attaagaaag taccttcaag aagaatacaa
      241 catttatgga cataacggca ctaaaaaagg agaagaatat ggtcataaat caaaatttta
      301 ttctgggttt aatattggta aagtaacgtt ccatttaaat aataatgaca ctttttcata
      361 tgatttattc tacacaggag atgatgggtt accaaaaagt tttttaaaaa tttacgaaga
      421 caataaaact gtagagtctg agaaattcca tttggatgtc gatatttctt ataaagaaac
      481 gatataaatc tattaattat gtcataatca ttattttccg ggtagcatgc ctacccttat
      541 tattttttgc caattttgag gagggagaag caaaatgtgg ttagaaaaat ttaaaaataa
      601 gaataaagaa actaaatata gatattacga gaagtacaaa gaccctctca ctgccaaatg
      661 gagacgtgtt agcgtggtac ttaataagaa tggtaagcaa tcacaaaaag aagctcaaag
      721 actcttaaat gagcgtatag aagaaaaact gaatgataag aaacctacta cacttaagtc
      781 actaactttc catgtcgcat gtgatgagtg gtttcagaat tatatcaaaa cgtctgggtc
      841 aaaaagaaca actattaaaa ctaaattgag taagctaaac actttgaaga aatttgtaga
      901 tgaagatatt ttaatcaata aaataacact ttcatatgcg caacaagttt tcgatgaaat
      961 ggatagtaaa ggttatgtat atcaagtaaa caaagatgcg ttaagcatat tcaaaaatgt
     1021 atttgaatac actagacgta tctataaact gcaagattta gaatttttaa aagatataac
     1081 attaaataaa agaattaagt cttatgatga agtgaaagct aaacgtaata agtacctcga
     1141 attaaatgaa atagaatcta tcatcaaaga tattaatacg aaggctcaga agatgcactc
     1201 aggtatccat aagcggtttt atttattcgt tgcactcatg acagaattcc aagccttaaa
     1261 tggtatgcgt attggtgaaa tgttagccat tcaaaatgaa gatatagact ttgataataa
     1321 gagtttaaat attaacggaa caatccattg gtttcacgat gaatctggtg gattcggtgt
     1381 aaaagatacc actaaaacag agtctagtta tagaacaatt ggattgagta gcagaagttg
     1441 cgagatatta aagaaagcaa tactggagaa caaaaaggat tcaaaatgga atgatggata
     1501 tctaaataga aattttgtat ttactaatca taaaggcaac ccaatgcaga ctgaaagatt
     1561 caataaaatc cttagggaag cagctaaaga tgttggtatt gataaagaag tttcttcaca
     1621 tatattaaga catagccaca tatcattact ctctcaacaa ggcgtgtcac ttaaagctat
     1681 tatggatcgt gtaggccatt ccgaccaccg tacaacactt tccatttatt ctcacgttac
     1741 tgaacagatg gataaggata tgatgaacaa acttgaacag gtaaaacttg gataatgcgc
     1801 tcaaatctga gcgtattttt ttgtttaaaa ttcgataatt tgcatatact gccccttttg
     1861 tgcccttttt tattttcaat accacaaaaa taacccctta atcgttgtta ttaaagggta
     1921 tttttattta attggtatta tttagcagga ataactgcac cattgtattt ttcattaatg
     1981 aagtcttgaa tatctttaga ttgtaatact tcaattaatg ctttgatttt cttatcatct
     2041 tgatgtcctt ctttaacagc aattaagttt gcataaggat tatctttcgc actttctaca
     2101 gcaatagaat cttttttagg atttagtttt tgttcgattg caaagttcga attaatgata
     2161 acagcatcag cgtcttcatt ttgataaatt ttaggtaaga attctgctga ttgtttatta
     2221 ttaaacttaa tatctttttt attctctgta atatcactaa acttagcatc ttcaattttt
     2281 acgccttttt tgattttaat taaacctgca tcaacgaaga attttaagaa acgtccttgt
     2341 tcagctggat tattagacac atagactgtt gcaccttttg gtaattcttt taaactttta
     2401 tactttttag agtatacagc cataggttct aagtgaacat caccggcact tacgattttg
     2461 taacctttat cctttttctc tgtgtttaaa tatggtgtat gttggaaata gtttgcgtca
     2521 atttcacctt tgtctagtaa tttattaggt gtagtgtaat cgttaattgt tttaatatct
     2581 agttcataac cttttttctc taataatggt tttgcttttt ctaaaatttc agcatgtggt
     2641 gctggtgaag cacctactgt tattttcttg tcgtcactgc cacttttgtt tccattaccg
     2701 caagctgcta atacaactgc aaatgttaat actaaaataa gaccaaataa ttttttcata
     2761 aaatgaaacc cccaatttat cgtttatcaa gtttatttgt aagccaatcc ccaatgaatt
     2821 ggattataaa tacaataatt aaaataaaaa ctgttgatac taaaatgaca tcattttgat
     2881 ttcgagtgaa acctgttaag tatgctaaat ttcctaaacc accggcacca attacacctg
     2941 caactgctgt tgaaccaact aaagcgattg ctgtaactgt aatgccagac actagcgctg
     3001 gcatagcttc aggtaaaagg actttacgaa ttactgtcca agtattagcg cccattgacc
     3061 aagccgcttc gatgacacct ttatcaattt ctttaaaagc aatttctacg agccttgcat
     3121 aaaacggtgc tgcgccaatg atcaaggctg gtaacgcacc tgtcggacca cttatcgttc
     3181 caagtatcaa acttgtaaat ggaattaata ataaaattaa aataataaat ggtatcgctc
     3241 taaataagtt aacaatgaaa gaaacgatag aataaaataa ccttgcaccg atagacttac
     3301 ctttagcaga caagaataat aacacaccta aaataagacc aagtataaat gcaaatatag
     3361 ttgagacgac tgtcatgtat agtgtttcga ctattgcagt ccaaacttct ggccactgaa
     3421 tattaggcat tgtaatcatt tcatttataa tttcactaaa tgatttaccc atgtcttaac
     3481 acctccattt taacttgtcg ctcaattaac tctttttcga attttccgaa atctacactt
     3541 gaaatatatg gaatatgcag aactaaaaag ccgactgttc cattttttgt atttttaata
     3601 tttgcttcta aaatattaat tttaatatca taggcagttg atagactcga tacaataggc
     3661 tcggttgttg ttgaaccagc gaaaactaat ctaacgatat atgcatcttt ttctaatggc
     3721 tctaattctg ttaaagatgt ttcgaaatca tcatttaaat cgtctttcac aaatcgtttt
     3781 gtcacagtgt gttgcggatt ttcaaaaacc tgtgtcaccg gtccttgttc tatcacttta
     3841 ccactttcca taactgcaac ttcatcacaa atacgacgaa tgacatgcat ttcatgcgta
     3901 attagtacaa ttgttaaatt ttgttgttct ctaattttta gtagtagatc taaaatttca
     3961 tctgttgttt gcggatcaag tgcacttgtt gcctcatcac aaagcaagac cgttggatca
     4021 ttagctaacg ctcgtgcaat cccaacacgt tgcttttgtc cacctgataa ctctgatgga
     4081 taagcctttt ctctaccttt taaaccgacg agttcgacaa gttctaatgc tttttgctta
     4141 gctctccttc tagggacacc tgcaatttca agcggaaaca taatattttt taacacagtc
     4201 cttgaccata acaaattaaa atgttggaag atcatactta ctttttgtct ttttgctctt
     4261 aatccatttt tggacaattg acctatatgg tctccatcta taataacttc acctgatgta
     4321 ggcgcttcta aatgattaaa cattcgaatc aaagtacttt ttcctgctcc agaaaaacca
     4381 atgacgccat aaatcgatcc tgctcgaatc gataaattaa cgtgatctac agcaaggact
     4441 tctttatttt tagtccgata ttctttaaca acttctttta attcaatcac gttgattcct
     4501 ccctgtgttg cttaataaaa taaaaaatgc tttctcaata tcgatagaaa aattgagaaa
     4561 gcaatagtag tattgtttct ctcatcttca aaagttaaaa ctttatgtga attggcacca
     4621 tttctatata agacggttgc cgggcttcgt agggcacatc cctccaccac tctcgataag
     4681 agtttacgca tcatttaatt tgtattaatc ctaacacctt agtaaaattt cgtcaataac
     4741 tattttaaat tttctaacaa atcagtcacc gatttaaatg cataaattcg ttttacttct
     4801 ttatctttat tcatcaacaa taaaatcggc gtagacatga tttgcatatc tttacaaaac
     4861 tgaggataaa agtttaaatc tattttcaat aatggtaact gcaatatttc attagcaatg
     4921 tctaacattc tttctgaaac cttacaagta ccacacgttg gtgtataacc aaagattaaa
     4981 tgtttgtctt cctcataaaa tgtagttaca tctttgatgt ctaatgaatt attcatttac
     5041 taaaactaac ctttcattat ttatattcgg taaaagaggt gtttctttct tacaagtaaa
     5101 gccatgtttt gaaagtacat gcgccaaata ttgtttgggg caattcgcaa cttgacagta
     5161 agttttatca ataaatatat gttcactttc actcaaataa cgtttaaacc aatttctaat
     5221 tcgatctcct tcgtcatcag aatcggctaa tacaaaaact tgtttatcat acagtgattc
     5281 tatcatatca tcaagcttat ctatactcat tgttccatga gtacaaataa tattgactgg
     5341 ttctgcaata acctgttgca cccttttttt atcagatttt ccttcaacaa ttatcacttt
     5401 atttacaata gccatcatca tcacccttta aaatcaataa acatctgtca ctgtatcatt
     5461 tcacaaaatt ggtatgaata aaacataaat cacaaaaaat ttaaactagc ttaatataat
     5521 aattacaaac tcaatgtttg actagctgga acatttaaca taagcagaca aaggctaagt
     5581 caaaaatcaa catcctaaaa tctacaatgt tatattaaca atagttaacc aaaagaaaat
     5641 acacctataa caaacttttc aattatagcg gggccccaac acagaagctg atggtaagtc
     5701 agcttacaat aatgtgcaag ttggcggggc cccaacataa agaaatactt tttctttaga
     5761 aattagtatt tcttatgcat gagttttact catgtattcc tatttttaaa tacacattag
     5821 ctgtggctta tgaaaacagg ctgggacata aatcaatgtt ctatgctcta cgaagttata
     5881 ttggcagtag ttgactgaac gaaaatgcgc ttgtaacaag cttttttcaa ttctagtcag
     5941 gggccccaac acagagaatt tcgaaaagaa attctacagg caatgcaagt tggggatggg
     6001 ccccaacaaa gagaaattgg attcccaatt tctacagaca atgcaagttg gggtgggacg
     6061 acgaaataaa ttttgcgaaa atatcattta tgtcccactc cctagattga tctatagata
     6121 ctacacttat taaagtaata tatttttatg attctcttag ctgcaatccc atgaatacat
     6181 gtaatcatca aacttcatag cctcaaggtc agtagatttc actaagatat tggcaatacc
     6241 taaatcaccc catataatcg aagaattacc atctcttgta gaatcaattt gcagtaagac
     6301 tctatcatac attttcagtt cttgatcgtt cgttcttgga tctatttggt caaaatatgg
     6361 ataaccacca agttgattaa atggttcctt agcagtaaat gtgttacaca caggttccat
     6421 caattcatca taaccaccaa acattgattt tagcgcgaag gatggattga ctttattcca
     6481 caatgcaagc acgtcctcat cgtacttata atcagtacat gtaatagttt gtttcgacaa
     6541 tctaaattct atagaaaatg ctccactgaa cgggatatcc tcattatcag catctaatga
     6601 cttcaaatgc gcttgaatat tttcgagtga taaatctgta attggctctt ttatataaac
     6661 aagccttgag tttatattgt ttccctttaa attttcagac attccataca tttgatcttc
     6721 tgaactaatc caaaactgca atatcccttc ttttacagga aataattctt gttgttctgg
     6781 aagatcatcc aaattaatct gtgctagcaa taccattggt acattatttt catctaatgg
     6841 tatatcttta aaatatttta aaaatggcat tccaccaatc ttactctggt ttaatgtttc
     6901 ttcttgcatc tcttttagat ccatttcaat acatggtagt actggtaaat taccgttttc
     6961 tacagattct acgaaacggt tatatgcttt ttgctccttt ttagaaagat gactaacgtc
     7021 ttgaatgttt agcatattaa cttcccccaa tttttatttt taaaatagct aaattcatta
     7081 tttccatcat tcttgaataa taaaatgtag caatactcca aataatagta cctaataatt
     7141 tttctttcat atcatttatt ttctcagact aaaaataaac tcctcaatct tagattaagg
     7201 agttacacgg tgattattca ccaatcattt ctgaatattt ttcagctgtt aataaagctt
     7261 caatctgact ttcatcacta atttctactt ttaccatcca tgctttttcg tatggagatt
     7321 catttacaaa ttcgggacta tcttctagtt cttcgttgac ttcaactact ttaccagaga
     7381 ttggtgcata taattctgat acagttttaa ctgattctac gctaccaaac gtatcccctt
     7441 cattaatttc atcatctgtt tctggtaatt caacgaaaac aatatcacct aactcgcttt
     7501 gtgcgtattc tgtgattcca attattgcta cattaccttc aactttaacc cattcatgct
     7561 cttttgaata tttcaattca tttggtactg ccaagagaat cccctcctaa ttaatcttta
     7621 catcattaat gatgccataa ggctagatgc tattcaacta tatttataaa aattaacaat
     7681 tgtatagctt atttcatttt acatgtaatg aatctaaata tcgagatatt tagtaaacgc
     7741 ttacatttca cttacgctaa ccaagtctct ttatattgat cttctttaaa tcctaatgtt
     7801 atcttatcgc ccattactgc tagaggacgc tttactaaca taccatcaga tgataacaac
     7861 tctaactttt catcatctga taaagtttgt aatttatttt tcaaatcaag ctcacgatat
     7921 ttcgcgccgt gtgtattaaa caatttatta atttctacgc ctgtatttgc aattattgtt
     7981 ttaaattcat ttattgtagg tgtatgttga acgatatcaa ttggttcata acttacgcca
     8041 tattcatcta aaaactttgc tgccttttta caagttgtac aattcttata ttggtaaaat
     8101 ttaatcatac gactgcaac
//
LOCUS       DAKTLH010000116         8103 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.116, whole genome shotgun sequence.
ACCESSION   DAKTLH010000116 DAKTLH010000000
VERSION     DAKTLH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.116"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            208..1710
                     /locus_tag="O0394_002092"
     CDS             208..1710
                     /locus_tag="O0394_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCY0500160.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSGQSWQAYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECADYDI
                     DFKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDI
                     YNVVINATGAKSHLNELDQDDQLIRNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSDNQ"
     regulatory      1929..2064
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2191..3234
                     /gene="ribD"
                     /locus_tag="O0394_002093"
     CDS             2191..3234
                     /gene="ribD"
                     /locus_tag="O0394_002093"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCY0500161.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     gene            3241..3873
                     /locus_tag="O0394_002094"
     CDS             3241..3873
                     /locus_tag="O0394_002094"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCY0500162.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFNQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            3884..5065
                     /gene="ribB"
                     /locus_tag="O0394_002095"
     CDS             3884..5065
                     /gene="ribB"
                     /locus_tag="O0394_002095"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCY0500163.1"
                     /translation="MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            5078..5542
                     /locus_tag="O0394_002096"
     CDS             5078..5542
                     /locus_tag="O0394_002096"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCY0500164.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(5664..6665)
                     /locus_tag="O0394_002097"
     CDS             complement(5664..6665)
                     /locus_tag="O0394_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HCY0500165.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVTGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            6777..6896
                     /locus_tag="O0394_002098"
     CDS             6777..6896
                     /locus_tag="O0394_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500166.1"
                     /translation="MMLISFIYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            6899..7726
                     /locus_tag="O0394_002099"
     CDS             6899..7726
                     /locus_tag="O0394_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0500167.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
BASE COUNT         2900 a         1156 c         1484 g         2563 t
ORIGIN      
        1 tataaatttc aatttaattc acctcgttaa aaatatttta acacaaacac taaggttgat
       61 attaaatgaa aagttttaaa tggtttaatg gttgtgaaag tgaaataata gcttacctta
      121 ataaatttta ttggaaatac caaattgcta taattatagg taatagttta aatataatat
      181 tttgaattac aaaaggatgg atgatatatg cgtgtagcaa taataggcat gggaactgct
      241 ggtgtaagtg tattgagaga gttagttaag cacccgaagt ttaatcaatt agatatagat
      301 ttatacgatg ataaagtaaa tatgggacag ggcgtaccat ttcaaaatga tagttctgag
      361 ctgctaatta atatgccttc gaaaaagatg agtttaaatt tagatgatga gactgaattt
      421 tggaagtggt ataaacaaca aactgatttt aattttgatg aacctgcata tttaccgaga
      481 tttgtattcg gacattatat gaaatcttat ttatctatgt tcacgaaaaa atatccaaat
      541 atatcaacta attataataa agttcaagag atttatacaa attctaatat cgatgaaacg
      601 aatttaactt attacatatg tactacaaat tcaggacaat catggcaagc gtacgattat
      661 gtatttttaa catgtggtac atttgcctac catgatccat ataacttaaa gggtaaaaaa
      721 ggatatatcg ctacgcctta tccaacatat aatacgttag acgaagtaaa tgaactggac
      781 gatattgcga ttattggtac aggccttgcc agtttagatg ttgtccgtta tgttgctgct
      841 catcatccaa aattaccaat tactatgacg agtcgttctg ctcatttacc aagtgtaaga
      901 ggtacaatga tagacgtgac attcaagtac ttaactaaag acaaattaaa tgacattaaa
      961 aaacatcatt tcggcaatgc accgcttgat actttagttt ctttattttt aaaagaatgt
     1021 gctgattatg acattgattt taaaaaatta gtacatagac gtacaggaaa tcatattgca
     1081 gatttgaagt atgatttagc gcgtccaaca gaaatgggca ttttccaaag tatgattgaa
     1141 catttgaaag agaatttaaa ttggatatgg aatagcttaa gtattgaaga ccaacatcaa
     1201 tttaatcaaa aatactcaaa gatgattcaa ttaaattcaa atccaatgcc tccaagaact
     1261 gctgaactaa ttattgaatt aatagaaaat aagtctttaa ttttgaaaaa agatttagaa
     1321 gatgtgaaac atgatggcaa gttgtattat ttttcataca aaaaccaaga atcagtagac
     1381 atatataatg ttgtcattaa cgcgacaggt gcaaaaagtc atttaaatga attggatcaa
     1441 gatgatcaat taattagaaa tcttgaaaat agacaaattg tacaagcaca cccaatggga
     1501 ggcatacaaa tcattccgga aaccaatcaa gttattagtc caaggtttgg aacattaaca
     1561 aatatgattg cgataggtca aatgacaaac ggtgttaata aactacgaaa cggtgtgaaa
     1621 atgattgtag aacaagttgc gcatacagta tctcaattat atgatgcttt agaatcgaat
     1681 gagcaacaac agcgcagtga caatcaataa tttgtaacta gaagataata aagagaacgc
     1741 tctatagaga cgaattgaag gtttgatttt aatgtctgtt agtaagaatc atatcaatga
     1801 gatgcctata gtactcagat tatattaaat taaaaccgtc attaattgtt ttttcagaaa
     1861 acatatagta tcattttaaa tgtagttgac atactacgta ctcaaataat ctataacaat
     1921 ttcatatata attctttcgg ggcagggtga aattcccaac cggcagtaaa taaagcctgc
     1981 gacctgctaa tatgtttcat attagtggct gatctagtga gattctagag ccgacagtta
     2041 aagtctggat gggagaaaga atgttaatta tcgacaaaga taatgtagcg tatttgtaaa
     2101 aatgtgtaca aataggctta tttaacgata aatttttctc cttgcatctt aattcatgat
     2161 gtgaggattt ttgtttatag aggtgatcat ttgagtcaat ttatggatta tgcgattcaa
     2221 cttgcaaata tggtacaagg tcaaacaggt gttaatccac ccgttggcgc tgttgtagtt
     2281 aaagaaggta ggattgttgg tattggtgca cacttgagaa aaggtgacaa gcatgcggag
     2341 gttcaagcac ttgatatggc acaacaaaat gctgaaggtg cgacgattta tattacgtta
     2401 gagccatgta gtcattttgg ttcaacacca ccctgtgtta acaaaataat tgattgtaag
     2461 atagcaaaag tagtatacgc aacaaaagac aattcgttag acacacatgg tgatgagacg
     2521 ttacgggctc acggtattga ggttgaatgc gttgatgatg aacgggcatc acaattatac
     2581 caagactttt ttaaagcaaa agcaaagcaa ctgccacaaa ttacagtgaa agtatctgca
     2641 agtttagatg gtaaacaagc gaatgataat ggacaaagtc aatggattac taacaaagag
     2701 gttaaacaag atgtctataa gttaagacat cgacacgacg cagtgttaac tggaagacgt
     2761 acagttgaat tagatgatcc acaatatact acacgtattc aagatggaaa aaaccctata
     2821 aaagtaatat tgtctaagtc tgggaatatt cattttaatc agcaaattta tcaagatgaa
     2881 tcaacaccaa tttggatata tactgaaaat ccaaatttaa caaccaatca aacacatatt
     2941 gaaattattt acttgaagtc ttgcgattta acaacaattc ttcacaattt atataaaaga
     3001 ggagttggaa ctttgctagt tgaggcaggt ccaaccacta cttcagaatt tctccaatct
     3061 aattatatag atgaatttat tctctattat gccccgaaat taattggcgg atctggaaat
     3121 tatcaatttt atcaaacaaa tgatgtgatt gagataccag atgcgaacca atttgaaatt
     3181 gttcattccg agttattaaa tcaaaatgtt aaattaactt tacgaaagaa gtgatgatgc
     3241 atgtttactg gcatcgttga agaaataggt gtcgttaaaa gtgttcaaat tcgtcaatca
     3301 gtcaggacga ttgaaattga agcacataag attacggcag atatgcatat tggtgattcc
     3361 atcagtgtta atggtgcatg tttaacagtg attgatttta atcagacatc ttttactgtt
     3421 caagtaatta aaggcactga aaataaaacc tatttagcag atgttaagcg acaatcagaa
     3481 gtaaatttag agcgtgccat gagtggtaac ggtaggtttg gtggacattt tgtcctcggt
     3541 catgtagatg aactaggaac agtttcaaaa ataaatgaaa cagccaatgc aaaaattatt
     3601 acgattcaat gtagccaaca tattaataat cagttagtta agcaagggtc tattactgtg
     3661 gatggtgtta gtctaacggt atttgataag catgataaca gttttgacat tcatcttatt
     3721 ccagaaacga ggcgttcaac gattttatca tccaaaaaat taggagataa agtacattta
     3781 gaaacagacg ttttgtttaa atatgttgaa aatattttaa ataaagataa agaccaatta
     3841 tctgtagata aattaagagc atttggtttt taggaggggt agcatgcaat tcgataatat
     3901 tgacagtgct ttaatggctt taaaaaatgg agaaccaatt attgtagtag atgatgagaa
     3961 tcgtgaaaat gaaggtgatt tagtagcggt tactgaatgg atgaacgata ataccattaa
     4021 ttttatggcg aaagaagcaa ggggattaat atgcgcacca gtgtctaaag atattgcaca
     4081 acgtttggat ttggtacaaa tggttgatga taactccgac atctttggta cgcaatttac
     4141 agtgagtatt gatcatgtag atacaacaac aggaattagt gcttatgaac gtacattgac
     4201 tgccaaaaag ctcattgatc ctagtagtga agctaaagat tttaatcgtc ctggtcattt
     4261 atttccatta gtagcacaag ataaaggcgt attagctaga aatggacaca cagaagcggc
     4321 tgttgattta gctaaactta ctggtgccaa gcccgctggt gtcatttgtg agattatgaa
     4381 tgatgacggc acgatggcga aaggacaaga tttacaaaag tttaaagaaa aacatcaatt
     4441 aaagatgatt acgattgatg atttaattga atatcgtaaa aaattagaac cagaaattga
     4501 atttaaggca aaagtgaaaa tgcctacaga tttcggaaca tttgatatgt atggttttaa
     4561 agcgacatac acagatgaag agatagttgt actgacaaaa ggtgcaattc gacaacatga
     4621 aaatgtacgc ttacattctg cgtgccttac aggcgatatt ttccatagtc aacgttgtga
     4681 ttgtggtgct caacttgaat cgtctatgaa gtatatcaat gaacatggtg gcatgattat
     4741 ttatctacct caagaaggtc gtggcatagg attgttaaac aaattacgcg catatgaatt
     4801 aattgagcaa ggatatgata cagtaactgc aaatttagca ttaggttttg atgaagattt
     4861 gcgagattat catattgctg cacagatttt aaaatatttt aacatcgaac atatcaattt
     4921 attaagtaat aatccaagta aatttgaggg attaaaacaa tatggcattg atattgcaga
     4981 aagaattgaa gttatcgtac cagaaacggt acataatcat gattatatgg aaacgaaaaa
     5041 aataaaaatg ggtcatttaa tataggagga ttttaacatg aattttgaag gtaaattaat
     5101 tggaaaagat ttgaaagttg caatcgtagt tagtcgattt aatgatttta tcactggaag
     5161 attacttgaa ggtgcaaaag atactttgat tcgacatgat gttaatgaag acaatattga
     5221 tgtagcattt gtgccaggtg cgtttgaaat tcctttagta gctaaaaaat tagcctcatc
     5281 aggaaattat gatgcagtaa ttacattagg atgcgtaatt cgcggtgcta cgtctcatta
     5341 tgattatgtt tgtaatgaag tcgcgaaagg tgtttctaaa gtgaatgatc aaactaatgt
     5401 accagtcata tttggtattt taacgactga aagtattgaa caagctgtgg aaagagcagg
     5461 tacgaaagct ggcaataaag gtgctgaagc agcagtaagt gcaattgaaa tggctaattt
     5521 attaaaatct ataaaagcat agagcactgt tgtgagttca taatcaaaga tttataaatg
     5581 tttcagaact gccaatataa catttaggag cctagaacat tgattattgt tccagactcc
     5641 taaattattg tttatgtctt gctctattta cggcataatt ttttaattgt acttaaacct
     5701 aacataactg tagctcctaa agctgcaatt atgccaacac gttttaagcc agcaggtttc
     5761 acaaattctt ttacagcaag agataggttt tgtgggcgtt ctgctaatct tctcataaaa
     5821 tacgcaaacc aatcatcgcc ataaggtaca taaatagtga aattatagcc ttcatttgcg
     5881 atttcttctg ctaactctga tctaaaacca tagagcattt ggaattccat acgatctttt
     5941 tcaatgtgat tttctttcat aaattgtttt acatgattaa tgatgcgatg gtcatgtgtt
     6001 gcaattgaag tgaaattgcg tgcgtttaac aaacgttgtt caattatttt gatgtaattt
     6061 gcatctacgt cttccttaga ttgaaatgca attgattcgt tttctttata tgcaccttta
     6121 accaaacgta atcgtaaatc ttggtactta tcaactaatt cgtggctatc gtataaatat
     6181 gcttgaatta cagtaccaac atttctaaat tcgcctttta agcgatctaa aacttgaaca
     6241 atttgttgca ggctagcata tttttcagta tcaatattta tatgcatatt gttgtaagta
     6301 tttgctttaa gtaaaatctc tcttaaattt tggtaagcta attctaagtc gaattctgca
     6361 cctaactgac tcaatttaac agacatatgt gcctttacgc catgttgatg aagcgcgtcc
     6421 ataattgtta aaatttgttc tttagcatga ttactttctt caactgtacc gacaaattcc
     6481 cctaaattgt ctaccgtaac agcgatattc ttgtcattta agtattcgat tgtattaatt
     6541 aactctggaa ttgtatttcc ggtaacgact ttattggcgc ccaaacgtgg gcccactttt
     6601 tttgctgcgt tgtttaaaaa actattatta gataatccga taaaaaaatt ctttaatagt
     6661 gccattgcta ctcctccaaa cccttgtgtt tacaataagt ttagcatgaa aaaaatattc
     6721 gagaaagcgt tttcatcaaa aacaatcaaa aataattatg tataattaca ctattattga
     6781 tgttgatttc atttatatat aataatatgg acaacaataa tactataaat agaaatctac
     6841 atagtgaatt tgctataatt atgttattga agattaatga aaaaggggca gtataataat
     6901 gtggaagtgg gaagctgaaa atgatgcgaa aggcgtcgtt gttattgctc ataatatatt
     6961 agaacatacg ggaagatatg catatgttat tacgatgtta agaagaaatg gctatcatgt
     7021 gattatggga gatttgcctg gtcaaggtca aacttcaaga gctaataaag gacaaattga
     7081 aaactttcaa acgtatcacg aaagtttgct agactggtta aagatagcta atgaatataa
     7141 aattcctact tatgtattag gggtaggact tggcggtttg attttattaa atttacttga
     7201 aaaagtagaa ttgccaatcg aggggatgat gttaatttct cctatgctag aacttcaaaa
     7261 gaatggaaaa aatcgtaaag ataaacttgt ttcaaatata ggtaaaattt caaaagacac
     7321 acgttttaat gttggtgtag aaccaaaaga tttaacacgt aatttagaaa ttgttgaaga
     7381 aacagttaac gatggtttaa tgttaaaaaa agcaacgtat cattggtata atacaattaa
     7441 tgagacgatg aaagatacga tggctcatat tcatgacatt caaccaatgc caacgctatt
     7501 aatgtatgga acgaaggatt taattgttga tactagggca attgatgagt ttaaagaaaa
     7561 atatcaaaca cctgaattat attttaaagc ttggcaaggt ttttaccacg aggttcacaa
     7621 tgagccagaa cgtgatgaag taatgcgtta tattctgact ttcttaaata acagcgtcaa
     7681 tacaatgggc tttattgttg aagatgacga aattgtagaa atttaatatt ctaaaaaggt
     7741 tgggacataa atccctaaaa aacagcagta agataatttt caattagaaa atatcttact
     7801 gctgttctct atttatacaa tacttcgtat tgaatggctt cgctttccta gggtgccgtc
     7861 tcagcctcgg tcttcgactg gcactgctcc ctcaggagtc tcgccattaa tactacgtat
     7921 taacatgtaa ttttactttg aaatacttta aaaaaataag acactttgcc caacttacac
     7981 taccaataaa aacttctgtt agaattcctc aaaatgatat ttcgcgacat gttaatgaaa
     8041 ttgttgaaac gatacctgat agcaaattcg atgaattcag acatcatcgt ggcgcaacat
     8101 cct
//
LOCUS       DAKTLH010000117         8073 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.117, whole genome shotgun sequence.
ACCESSION   DAKTLH010000117 DAKTLH010000000
VERSION     DAKTLH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.117"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            39..830
                     /gene="pgeF"
                     /locus_tag="O0394_002100"
     CDS             39..830
                     /gene="pgeF"
                     /locus_tag="O0394_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HCY0500168.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAAL
                     LIYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            854..1522
                     /locus_tag="O0394_002101"
     CDS             854..1522
                     /locus_tag="O0394_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCY0500169.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            1519..2082
                     /locus_tag="O0394_002102"
     CDS             1519..2082
                     /locus_tag="O0394_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCY0500170.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            2094..2384
                     /locus_tag="O0394_002103"
     CDS             2094..2384
                     /locus_tag="O0394_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCY0500171.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            2494..3273
                     /locus_tag="O0394_002104"
     CDS             2494..3273
                     /locus_tag="O0394_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCY0500172.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            3297..3914
                     /locus_tag="O0394_002105"
     CDS             3297..3914
                     /locus_tag="O0394_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCY0500173.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTPDNNQQSNDSETTKK"
     gene            4135..6888
                     /gene="ileS"
                     /locus_tag="O0394_002106"
     CDS             4135..6888
                     /gene="ileS"
                     /locus_tag="O0394_002106"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCY0500174.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            7178..7975
                     /locus_tag="O0394_002107"
     CDS             7178..7975
                     /locus_tag="O0394_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0500175.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDILGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNNLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
BASE COUNT         2950 a         1137 c         1535 g         2451 t
ORIGIN      
        1 gacgaagata atttaaatga aatgatggtg acgatcgagt gaatgataat tttaaaaagc
       61 aaccgcatca tttaatatat gaagagttat tacaacaagg tattactcta ggtattacaa
      121 ctagaggaga tggtttaagt gactatccta aaaatgcttt taatatggcg agatatattg
      181 atgatcgccc atataatatt actcaacatc aattgcaatt agctgaagaa attgcgtttg
      241 atagaaaaaa ttgggtgttt cccattcaaa cacatgaaaa taaagtcgct tgtattacaa
      301 aggatgatat aggcacaaat atagacactt taactgatgc gcttcatggt attgatgcga
      361 tgtacacata tgatagtaat gtcttattaa cgatgtgtta tgcagactgt gtaccagtat
      421 atttttatag tacaaaacat cattttattg cattggcgca tgcaggttgg cgtggtacct
      481 atactgaaat tgtaaaagaa gtgctaaaac atgtgaactt tgatttgaaa gacttacatg
      541 tcgttattgg accatctaca tcatcaagtt atgaaattaa tgatgatatt aaaaataaat
      601 ttgaaacatt gccaattgat agtgccaact atattgaaac tagaggacga gatcgtcatg
      661 gtattgattt gaaaaaagcc aatgctgcat tattaattta ttatggtgtt cctaaagaaa
      721 atatttatac gacagcgtat gctacatctg aacatttaga attatttttc tcttatcgat
      781 tagaaaaagg tcaaacagga cgcatgttag cattcattgg tcaacagtaa acaaggagga
      841 gatatgtttg cgtgtgaaag ataatttaca acaaatctca acacaaatta atgacaaaag
      901 tgaaaaaaat aatttttcaa caaaaccaaa cgtgattgca gttacaaaat atgttacaat
      961 agagcgagct aaagaagcgt atgaggctgg aataagacat tttggtgaga atagattgga
     1021 aggctttttt caaaagaaag aagcattacc atcagatgcg gtgatccatt ttataggatc
     1081 attacaatct cgaaaagtta aggacgttat aaacgacgta gattatttcc atgctttaga
     1141 tcgattgagc ttagccaaag aaattaacaa acgtgcagaa cataaaatta aatgtttctt
     1201 gcaagtgaac gtttcgggag aagcttctaa acatggtatt gctttagaag atgttgatca
     1261 gtttatagat gatcttaaaa aatatgacaa aatcgaaatt gtaggtttaa tgacgatggc
     1321 accattgaca gatgatgaag catatattag atcgttattt aaacagttac gtttgaaaaa
     1381 agaagaaata caacgactca atttagaata tgcgccttgt gatgaattat caatgggaat
     1441 gagtaatgac tatcttattg cagttgaaga aggtgcgacg tttgttagaa ttgggactaa
     1501 acttgtagga gaagaggagt gagccacttg gctttaaaag atttatttag tggatttttt
     1561 gtaatagatg atgaagagga agtagaagta cctgacaaac aacaacaggt aaatgaagcg
     1621 ccagcaaaag agcagtcaca acaaacaaca aaacaaaacg caatcaaatc agtccctcaa
     1681 aaatctgcat caagatatac aacaacgtca gaagaaagga ataaccgtat gtctaattat
     1741 tcaaaaaata attcacgtaa tgttgtaact atgaacaatg ctacaccaaa caatgcatca
     1801 caagaaagtt caaaaatgtg tttattcgaa ccacgtgttt tttcagatac acaagatatt
     1861 gctgatgagc ttaaaaaccg ccgtgcgaca cttgtcaatt tacaacgtat tgataaagta
     1921 tcagcgaaaa gaattattga ttttttaagc ggtactgttt atgcaatcgg tggagatatc
     1981 caacgtgtag gtactgatat tttcttatgt acgcctgata atgtggaagt agctggaagc
     2041 attacagacc atattgaaaa tatggaacat tcattcgact aaggagttaa catatggata
     2101 taaatgtgct agctacaata tttaaattca tcctttttgt tgttgaaatt tattatttcg
     2161 gcatgattat atatttcttt acatcttggg taccaagtat tagagaaact aaggtaggtt
     2221 attttttagc gaaaatatat gaacctttct tacaaccatt tagaaaagta attccaccta
     2281 ttggaattat cgacatatca tcaatcgctg caattatcgt tttagtatta ttccaaaaag
     2341 ggttactcca aatctttaat tggattttaa ttcaattaca ataaattaag cgacataacg
     2401 aggcaagggt acatgatact ttagcctcgt ttttgatatg catttttctg aatataaggg
     2461 caatagatgg tattttataa tttttttaag gtagtgatta acatagatat ttatcaacac
     2521 tttagacagg aagaatacga attaattgat cagctaacgg ataaatgtga tcaagcggaa
     2581 cagcattatg caccagtatt aacgcatttt ttagatccaa gagggcaata tatattggaa
     2641 gtgatttgtg gcagttatga agatttaaac gtatcttttt atggtggacc taatgctgaa
     2701 agaaaaagag caatcatttc gccgaactat tatgaaccta aagaaagcga ctttgaatta
     2761 actttaatgg aaatagatta tcctgaaaaa ttcgtcactt taaaacatca acatatttta
     2821 gggacattaa tgtctttagg tatcgaacgc gaacaagttg gagatataat tgtgaatgaa
     2881 cgaattcaat ttgttttgac aagtagattg gaatcattta ttatgttaga attacaacgt
     2941 attaaaggcg catcagttaa actttatact attccagtaa cagatatgat acaatctaat
     3001 gagaattgga aaaatgaaag tgcaacagtt agttctttaa ggttagatgt tgttattaaa
     3061 gaaatgatac gtaaatcacg tacgattgcg aaacaactaa tcgaaaaaaa acgtgttaaa
     3121 gtgaatcaca ctattgttga ttcagcagat tttcaattac aagcaaatga tttaatatcc
     3181 atccaaggtt ttggtagagc acacattact gacttaggtg gtaaaactaa aaaagataaa
     3241 acgcacatta cctatagaac attattcaaa tagtaatgat ttaaggagga taacaaatgc
     3301 cttttacacc aaatgaaatt aagaataaag agttttcacg tgtaaagaat ggtttagaac
     3361 ctactgaagt tgctaatttt ttggagcaac taagcactga aattgaacgt cttaaagaag
     3421 ataaaaaaca acttgaaaaa gtaatcgaag agagagatac taatattaag tcttatcaag
     3481 acgtgcatca atctgtaagt gatgctttga tacaagctca aaaagctggt gaagaaacta
     3541 agcaagctgc agagaaacaa gctgaagcga ttatagctaa ggcagaagcg caagctaatc
     3601 aaatggttgg tgacgcggta gaaaaagcac gccgtttagc attccagact gaagatatga
     3661 aacgtcaatc aaaagtattt agatcgcgtt tccgtatgtt agttgaagcg caattagact
     3721 tattaaaaaa cgaagattgg gattacttgt tgaattatga tttagacgct gaacaagtga
     3781 cgcttgaaaa tattcatcat ttgcatgaaa atgatttaaa gccagatgaa gttgcagcaa
     3841 atgcacaaaa taatgcatca aatacaccag acaataatca acaatccaat gattcagaaa
     3901 caactaagaa gtaagaatta aataaagaca gacgcgtaat atacatttaa cttttcacag
     3961 cgaattaggt aatggtgaga gcctagtaaa agcatgtatg ttatatcact ggctttttaa
     4021 tatttaaata atgtaatgag agaactctaa gttgagttaa taagggtggt accgcgagca
     4081 atcgtccctt ttaatttaac ttagagtttt ttaaattttt aaggagtgaa aaaaatggat
     4141 tacaaagaaa cgttattaat gcctaaaaca gatttcccaa tgcgaggtgg tttaccaaac
     4201 aaggaaccgc aaattcaaga aaaatgggat gcagaagatc aataccataa agcgttagaa
     4261 aaaaataaag gtaacgaaac attcatttta catgatggcc caccatacgc gaatggtaac
     4321 ttacatatgg gacatgcctt gaacaaaatt ttaaaagact ttattgtacg ttataaaact
     4381 atgcaagggt tctatgcacc atacgtacca ggttgggata cacatggttt accaattgaa
     4441 caagcattaa cgaaaaaagg tgttgaccga aagaaaatgt caacagctga attccgtgag
     4501 aaatgtaaag aatttgcttt agaacaaatt gaattacaga aaaaagattt tagacgttta
     4561 ggtgttcgtg gtgactttaa tgatccatat attacattaa aacctgaata cgaagctgca
     4621 caaattcgta tttttggaga aatggcagat aaaggtttaa tttataaagg taaaaagcca
     4681 gtttattggt ctccttcaag tgagtcttca ttagcagaag cagaaattga atatcacgat
     4741 aaacgttcag catcaattta cgttgcattt gacgttaaag atgacaaagg tgtcgttgat
     4801 gcagatgcta aatttattat ctggacaaca acgccatgga caattccatc aaatgttgcg
     4861 attaccgttc atcctgaatt aaaatatggt caatacaatg taaatggcga aaaatatatt
     4921 attgcagaag ccttgtctga cgctgtagca gaagcactgg attgggataa agcatcaatc
     4981 aaattagaaa aagaatacac aggtaaagaa ttagagtatg ttgtagcaca acatccattc
     5041 ttagacagag aatcgttagt gattaatggt gatcatgtta ctacagatgc tggtacaggt
     5101 tgtgtacata cagcaccagg tcacggggaa gatgactata ttgttggtca aaaatatgaa
     5161 ttgccagtaa ttagtccaat cgatgataaa ggtgtattta ctgaagaagg cggccaattt
     5221 gaagggatgt tctatgataa agctaataaa gccgttactg atttattaac agaaaaaggt
     5281 gcactattaa aattagactt tattacacat agctatccac acgactggag aacaaaaaaa
     5341 cctgtaatct tccgtgctac accacaatgg tttgcctcaa tcagtaaagt aagacaagat
     5401 attttagatg caatcgaaaa tacaaacttc aaagtaaatt ggggtaaaac acgtatttac
     5461 aatatggttc gtgaccgtgg cgaatgggtt atttctcgtc aacgtgtgtg gggtgtaccg
     5521 ttaccagtat tttatgctga aaatggcgaa attatcatga cgaaagaaac agtgaatcat
     5581 gttgctgatt tatttgcaga acacggttca aatatttggt ttgaaagaga agcgaaagac
     5641 ttactaccag aaggatttac acatccaggc agccctaacg gtacatttac taaagaaaca
     5701 gacattatgg acgtttggtt tgattctggt tcatcacacc gtggcgtgtt ggaaacaaga
     5761 ccggaattaa gtttcccagc ggatatgtat ttagaaggta gtgaccaata tcgtggttgg
     5821 ttcaactctt ctatcacaac ttcagttgct acaagaggag tatcacctta taaattctta
     5881 ctttctcatg gttttgttat ggacggtgaa ggtaagaaaa tgagtaaatc tttaggtaat
     5941 gtgattgtac ctgaccaagt ggttaaacaa aaaggtgctg atattgcgag actttgggta
     6001 agtagtacgg actatttagc tgatgttaga atttctgatg aaattttaaa acaaacatct
     6061 gatgtttatc gtaaaatcag aaatacatta agatttatgt taggtaacat taacgatttc
     6121 aatcctgaca cagatagcat tcctgaatca gagttattag aagtggatcg ttacttgcta
     6181 aatcgtttac gtgaatttac tgcaagtacg attaacaact atgaaaactt tgactactta
     6241 aatatttatc aagaagttca aaactttatc aatgttgagt taagtaattt ctatttggat
     6301 tacggtaaag atattttata tattgaacaa cgtgattctc atatccgtcg tagtatgcaa
     6361 acagtgttat atcaaatttt agttgatatg acgaagttgt tagcaccaat cttagtgcat
     6421 acagctgaag aagtttggtc tcatacacca catgttaaag aagaaagtgt tcacttagca
     6481 gacatgccta aagttgtaga agtagatcaa gctttattgg ataaatggcg tacatttatg
     6541 aatttacgtg atgatgtgaa ccgtgcatta gaaactgctc gtaatgaaaa agttattggt
     6601 aaatcattag aagctaaagt tacgattgct agtaacgata aatttaatgc atctgaattc
     6661 ttaacttcat ttgatgcatt acatcaatta tttatcgtgt cacaagttaa agttgtagat
     6721 aagttagacg atcaggcaac agcttatgaa catggtgata ttgtcatcga acatgcagat
     6781 ggtgaaaaat gtgaaagatg ttggaactat tcagaggatc ttggtgctgt tgatgaattg
     6841 acgcatctat gtccacgatg ccaacaagtt gtaaaatcac ttgtataatt gaaattgtat
     6901 aaagtactca tacagatgat ataaattaaa gctctcttca taatcatgtt gtagtttttg
     6961 ttgacatgat gaagagagtt tttttgtgaa taaaaaaatg accaagttac cggtcatata
     7021 tgtaaaaaat gtgcgattta ctaaaataaa aattattcag gaatggtaca aattctctga
     7081 ggcatataaa tgcgttatag ttgctattct caattatgtt cgcgataatt ttaagtaaaa
     7141 gtaagcacag atattgaatt tgataggagt taattgaatg tatcataaca gtaacgcaaa
     7201 ctttgtcaat ggtatcactt taaatgtgag agataagaat gaattaaagc cattttatga
     7261 ggacatatta ggattaaata ttataaatga gacattaaca tcgatacaat atgaagtagg
     7321 tcaaaataat catgtcatta cacttgttga attacaaaat ggacgtgaac ctttaatgtc
     7381 cgaagcggga ctgtttcata tcgcaattaa actacctcaa attagtgatt tagctaattt
     7441 actaattcat ttaagcgaat atgatattcc agttaacgga ggtatacagc ctgcttcgtt
     7501 atcattattt tttgaagacc cggaaggaaa cggttttaaa ttttatgttg ataaagacga
     7561 agcgcaatgg acgaggcaaa ataatttagt aaaaattgat attagaccat taaatgtacc
     7621 gagattagtg agtcatgcaa caaaattgtt atggttaggt attccagatg acgctattat
     7681 aggtgcattg catattaaga caattcattt atcagaggta aaagagtact acctcgatta
     7741 ttttggatta gagcaatcgg catatatgga tgattattca atatttttag catcgaatgg
     7801 ctattatcaa catttggcca tgaatgattg ggtatcagca acgaaacgtg tagaaaattt
     7861 tgatacgtat ggattagcaa ttgttgactt tcattatcct gaaacaacac atttaaattt
     7921 acaaggtccg gatggtatct attatcgctt taatcatatc gaagttgaag attagtatat
     7981 actttgaatg gacgaaccat ataatgaatc gtttttaatg atctttttat acaagttatg
     8041 aaggaggctg ggacattaag ttcttaggca atg
//
LOCUS       DAKTLH010000118         7865 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.118, whole genome shotgun sequence.
ACCESSION   DAKTLH010000118 DAKTLH010000000
VERSION     DAKTLH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.118"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(136..1290)
                     /locus_tag="O0394_002108"
     CDS             complement(136..1290)
                     /locus_tag="O0394_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCY0500176.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNPTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
     gene            complement(1507..2412)
                     /gene="yut"
                     /locus_tag="O0394_002109"
     CDS             complement(1507..2412)
                     /gene="yut"
                     /locus_tag="O0394_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HCY0500177.1"
                     /translation="MKIIDVLLKNISQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYTFARFINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKATLLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            2672..2974
                     /locus_tag="O0394_002110"
     CDS             2672..2974
                     /locus_tag="O0394_002110"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HCY0500178.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            2988..3398
                     /locus_tag="O0394_002111"
     CDS             2988..3398
                     /locus_tag="O0394_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HCY0500179.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGAENVNKKGGKRS"
     gene            3395..5110
                     /gene="ureC"
                     /locus_tag="O0394_002112"
     CDS             3395..5110
                     /gene="ureC"
                     /locus_tag="O0394_002112"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HCY0500180.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            5123..5575
                     /gene="ureE"
                     /locus_tag="O0394_002113"
     CDS             5123..5575
                     /gene="ureE"
                     /locus_tag="O0394_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HCY0500181.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            5568..6257
                     /locus_tag="O0394_002114"
     CDS             5568..6257
                     /locus_tag="O0394_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HCY0500182.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEGTRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            6270..6884
                     /gene="ureG"
                     /locus_tag="O0394_002115"
     CDS             6270..6884
                     /gene="ureG"
                     /locus_tag="O0394_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HCY0500183.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            6884..7720
                     /locus_tag="O0394_002116"
     CDS             6884..7720
                     /locus_tag="O0394_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HCY0500184.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
BASE COUNT         2679 a         1349 c         1323 g         2514 t
ORIGIN      
        1 tatttatttt ttattttcgc aaaaaattta acaaaatatc ttaacatccc tataaatccc
       61 cacaaaaaaa gggtaaatcc gaacgtcaaa tttcatctct ttatgccaat caacgatcga
      121 tttacctttt aaaatttaaa atgtatctgt aatactctta gcaatatttg tatatgctgc
      181 atcttccata ggtggattgt gtaatcctgc acgcatgtca cgatagtaac gttgtaatgg
      241 tcgattcatt tctaagcttt tagcaccaac tattctcatc gctaaatcaa tcacttcaag
      301 cccttgattc attaccatca ctttactcgc acttgttgga tttcttatct gactgtcatc
      361 tttatattgt tgataccctt ttgctgtact ccataaaaat tgtctcgcac ataacaatag
      421 cgtttccatt ttccctaaat tttgttgcac agttggcaat gttgcaatcg ttccttcaat
      481 actattaggg ctatgttgga ttgcaaagtc tacagcataa tttctagcag cttgtgctat
      541 acctaaataa cagcttggta tatgcaatat ccaaccattt ggcgctttac ttttctctgt
      601 ttccactaaa tgttttaatg gtacttttac atcatttaat attaaatcat gactttctgt
      661 cgctcgcata cctaatacgt cccaattatc ggcaatttca acaccaggca aattcctgtc
      721 tactaagaaa aaaccaacac tttctaattc ttctatataa gcagcaacaa taatatgtgt
      781 tagtgcttta ctcattgatg tatatgtctt cacaccattt aaaatatacc catcatcagc
      841 tttaacagca tgtgtacttg gtcttccccc tcttgttgga ctacccattt cagcttcact
      901 aactgctcta ttaactaatg caccattatt aatttcaaca gcaaattgct ccaacatatc
      961 ttgagaccat aatttctgtt cataaatttg tcctacgaca ctcacatgcc aaccaataga
     1021 taatgctgtg gcaccatcaa gttcgcctaa aaatgattgc aaaataacca tgtcttctat
     1081 ggtcgcacct tcaccaccat atgcttttgg taacgttaat tttccataac cttctttaat
     1141 taaccattca atatttttat atggaaattg gctatgttga tcattatatt cagctttagc
     1201 cttaaatgta tctctaatta ctttgaattt ttcaatccat tttctttgaa tttccgttgt
     1261 tattaaaaag gaattatcca tatcattcat attagtcacc ccacacaaca cacaatataa
     1321 attatttatt cctagtttaa cactcgactt taaaaataaa aaggaacgtg ctgtgaacaa
     1381 ttaaaagaaa actgataatt cgattaagta tgcacttaat tttatccata aaaatacacc
     1441 ttaaactata caattcgctg tatagtttaa ggtgatattc aaacaatatt aggtttgatt
     1501 taactatcaa gcatctactt tgtcatgttt aataccagca aataataaaa tccatgtcac
     1561 gataataaat ggcaatgtta atgcaggtaa gccaaacggt tcaagcaatg ttgttgtacc
     1621 tagttgcacc actactgtta ataacacacc taaaaaagtc gaaatataag ggttaatcgc
     1681 tgttttaaat gtatatccta gtgcgattgc cattaataca aagttataac cgaataatcc
     1741 ctgattaata tcatcataat taccacctaa tagagctaca atgataaagc ttaacaaact
     1801 agctataaca gctaagagtg ttgcttttct tgatgctatc aatatgccga ttaaaataca
     1861 tacgccacca attacactcg cttcgataaa tacttgactg aatccttcaa ataatgactg
     1921 aatgaaatgt attctatcat tgttgctaaa attaaccgtt tcaatgtttt gaggcattaa
     1981 ctttaacgat gtatctacaa atttaacctg tcctgaaagt aaaattgtaa accaagtcac
     2041 aatgacaaaa ggcatcgtca gcatcggaac tttatatggt cttaaaactt ctctcactgc
     2101 agcagcaact ggtaacgtta ataaagttgc tatcattgtt ataacaatat ctaatcctga
     2161 cttatctaaa aagattgtta acgcaatggc agttagcact ggattaaatc cagctaaccc
     2221 atcattaatc tctgcctcac tataatttat aaaacgcgca aaagtatagg cgatgatgct
     2281 acctacaata gccgctaatc caattgtcca atcggctaca aataatccta ttaagataaa
     2341 taatcctgtc catttattac taattaacac aacctgagat atatttttca aaagcacatc
     2401 tattatcttc aaatctttca cctttccttt gaatcattac tcattaatat actactattc
     2461 tatgaaaagt tatattaaaa actattttgg tttttacata aatatattcc actatttgtt
     2521 ttcgattaga ttgtttgctt caatatcgga tatgctaaaa atataatttt ctaattacac
     2581 attgaacttt gtatattact tgtttatcac aacaattagg attaatataa attttgtaaa
     2641 ttgtatctaa tatgaaattg ggggttttaa tttgcatttt acacaacgag agcaagacaa
     2701 attaatgatt gtagtggcgg cggaagttgc acgtcgtcgt aaagcacgtg gtttgaaact
     2761 aaatcatcct gaggcattag ctttaatcag cgatgaatta ttagaaggtg cacgcgatgg
     2821 taagaccgtt gcagagttaa tgagttatgg tagacaaatt ctaaacaaag aagatgtcat
     2881 ggatggtgtc gaacacatga ttacagatat cgaaatcgag gctacgttcc ccgatggtac
     2941 taagttaatc acagtacatc accctattgt ttaaaggagg cagtcaaatg ataccaggag
     3001 aaattattac aaaaagtaca gaggttgaaa ttaataacca tcatcctgaa acagttatcg
     3061 aagttgaaaa tacaggagat cgaccaattc aagtgggctc acattttcat ttttatgaag
     3121 caaatgcagc attagatttc gaacgtgaaa tggcatatgg aaaacattta gatattccag
     3181 ctggagcagc tgttcgattt gaacctgggg ataaaaaaga agttcaatta gttgaatatg
     3241 ctggcaaacg taaaattttt ggttttcgtg gtatggtcaa tggtcctatc gatgagtcac
     3301 gtgtctatcg cccaactgat gaaaatgatg aatatgcagg tgtattcgga gataacggtg
     3361 ctgaaaacgt gaataaaaaa ggaggaaaaa gatcatgagc tttaaaatga cgcaaaatca
     3421 atatacgagc ttatacggtc caactgttgg agattccatt cgtttaggtg atacgaatct
     3481 atttgctcaa atagaaaaag actatgcggt ttatggtgaa gaagctactt ttggtggtgg
     3541 taaatctatt agagacggta tggcgcaaaa tcctcgtgta acacgtgatg acgtgaacgt
     3601 tgcagacctt gtcatttcta atgccgttat tatcgattac gataaagtgg ttaaagctga
     3661 tataggcatt aaaaatggtt atattttcgc cataggtaat gccggcaacc cagatataat
     3721 ggataatgtc gacattatta taggttcaac aacagatatc attgccgctg aaggtaaaat
     3781 cgtcactgct ggtggtattg atactcatgt tcattttatt aatcctgaac aagcagaggt
     3841 cgcattagaa agtggtatta cgactcatat tggtggtggt actggtgctt cagaaggttc
     3901 taaagcaaca actgtaactc caggtccatg gcatattcat agaatgttag aagctgccga
     3961 aggtttaccg attaatgtcg gttttacagg taaaggacaa gcaacaaatc caactgcact
     4021 cattgaacaa atcaatgccg gagcaattgg attaaaagta catgaagact ggggtgcaac
     4081 accatctgct ttgagtcatg cattagatgt tgctgatgaa tttgatgttc aaattgcatt
     4141 acatgcagat actttaaatg aagcaggatt tatggaagac acaatggctg ctgttaaaga
     4201 ccgtgtactt catatgtacc atactgaagg tgctggtggc ggtcatgcgc ctgatttaat
     4261 taaatccgct gcattttcaa atattttacc ttcatctaca aatccaactt tgccttatac
     4321 acataatact gtagatgaac atttagatat ggtaatgatt actcaccatt taaatgcggc
     4381 tattcctgaa gatatcgcat tcgcagattc acgtattcgt aaagaaacga ttgcagcaga
     4441 agatgttctg caagatatgg gtgtattcag tatgattagt tccgattcac aagcaatggg
     4501 ccgtgtaggt gaagtaatta cacgaacatg gcaagtagca catcgcatga aagaacaacg
     4561 tggtccttta gatggtgatt ttgaacataa tgataataat cgcatcaaac gttatatcgc
     4621 taaatataca attaacccag caattacaca tggtatttct gaatatgtag gatctatcga
     4681 gccgggcaaa ctagctgaca ttgtcttatg ggacccaatt ttctttgggg ttaaacctga
     4741 attagttgta aagggcggat taattaactc tgccgtaaat ggcgatgcaa atggttctat
     4801 acctacatct gaaccgatga agtaccgtaa aatgtatggt caatacggcg gaaaccttac
     4861 aagtacgtca atgacattcg tgtctaaaac tgcttatgaa aatggtatca atcgtgcatt
     4921 aaatttaaaa cgcatggtgc gtccagttaa aaatattaga caattatcta aagcagatat
     4981 gaaaaataac agtgcaacac ctaaattaga cgttgatcca caaacatatg aagtatatgt
     5041 agatggagaa aaaattacaa gtaatgcagc aactgagtta ccattaactc aaagatactt
     5101 cttattctag gaggaacata gaatgattgt tgaagaaatc caaggcaata ttgccaattt
     5161 gtcaaattca gaaaaacaaa agcacgtcga aaaagtatac cttgaaaatt cagatcttgt
     5221 taaacgtatt caaagagtcg ttacagacca tggcactgaa ataggcattc gtttaaaaca
     5281 acctattgac ttacaatatg gagatatttt atacgcagat gaccataata tgattattgt
     5341 ggatgttaat tcagaagatc ttttagtgat tcaaccaaga acattgcaag aaatgggaga
     5401 tatagcccat caattaggaa atcgtcattt gccagcacaa tttacagaga ctgaaatgct
     5461 agtacaatat gattatttag ttgaagattt attaaaaagt ttaggcatcc catatgttcg
     5521 agaagaccgt aaagttaata aggctttcag acatataggg cattctcatg attgatcata
     5581 cacacttaag attatttcag ttctgtgatt cacagtttcc aacaggtgct ttcagtcatt
     5641 catttggtct tgaaacatat attcaacgaa atataattca tgacgatcat acttttattg
     5701 cttggttaaa aatgttttta caagagcaac ttacctattc tgatggttta gccatgcgtt
     5761 tagtttatga tgcattggaa aatgatgata cacaaaaagt attacacata gataaactga
     5821 tgtttgttca aaacttacct aaagaaacac gtgttggtgc aaaacaaatg gggactcgca
     5881 tggttaaatt ggctttagaa ctttataata gcccatggat tgcttggtat catcaacaaa
     5941 tgcaagataa gaaagcaaag ttaaatccag ctatttgttt tactatgcta ggccatcatt
     6001 taggtgtaga tattgaaacg attattgatt actatttata tcaaaatgtt tcaagtttaa
     6061 ctcaaaatgc tgttcgtgct attccacttg gacaaactgc tggtcaaaag atagttactc
     6121 acatgattcc ttatatagaa ggaacaagaa agcagatttt cgaattaaaa gaagcagatt
     6181 ttggcatgac agcgcctggt ttagaactaa atcaaatggc gcatgagaac gtcaatgtta
     6241 gaattttcat atcataggag gttataattg tggcaaatcc gattaaaatt ggtattggtg
     6301 gtcctgtagg tgcaggtaaa acacaattaa ttgaaaaagt tgtaaaacgt ctttcaaaag
     6361 aaatgagtat cggcgttatt acaaatgata tatatacaaa agaagacgaa aagatattag
     6421 taaattcagg agttctacct gaaagtcgta tcattggtgt tgaaactggt ggatgtcctc
     6481 atactgcgat tcgtgaagat gcatctatga actttgcagc aatagacgaa ttattagaac
     6541 gtcatgacga tatagaacta attttcatag aatctggtgg cgataactta gcagcaacat
     6601 ttagtccaga acttgttgac ttttcaatat atattatcga tgttgctcaa ggtgaaaaga
     6661 ttccacgtaa aggtggtcaa ggtatgatta agtcagattt ctttgtaatt aacaaaactg
     6721 atttagctcc ctatgtaggt gcatcattag aacaaatggc tgaagatact aaagtatttc
     6781 gtggtaaacg tccatttact tttactaact taaaaaccga cgaaggtttg gatgaagtta
     6841 tcgattggat tgaacgcgac actttactca aaggattatc ataatggatg aacaacaatg
     6901 gactgggcaa cttgatttaa cagtgttttt cgatggcaat cgatcagtat caagagatat
     6961 tttctttgaa aaagcactta aagtgatacg tccagtttat ctaaatcaat ctaccattcc
     7021 tacattttat atagtaaatg taggtggtgg ctatttagat ggagatcgtt accgtatgaa
     7081 tgtgaatgtc gaagataacg ctaaagtgac attgacatct caaggtgcaa caaaaatata
     7141 caagacacct tccaatcatg ttgagcagta tcaaactttt aatttgaaag ataacgcata
     7201 tttagaatat gtcgctgatc caatcatcgc atatgaaaat gctaaatttt atcaacacaa
     7261 tacgttcaat ctcaataatt ctagttcatt attttatact gatattttaa ctcctggtta
     7321 ttcaaaaact ggcgaagcct tcaagtatca atatatgcat ttaataaatg aaatttatat
     7381 tgaagatgag ttagtcacat atgataattt attattgaat cctaataaac aatcgatcaa
     7441 tgaaataggt tatatggaac attactctca ttatggctct gcttacttca tacacgaaga
     7501 tgttaaccaa aaactaatcg attcagttta tgaaaccatt agttcttata gcaatacatt
     7561 cgattgtcgt gttgctattt ctcaattgcc tacacatggt tttgcagtta gaatttttgc
     7621 ttatcgcaca caaattatag agaaaatact tggaaccatc caaagctata tcgcagaaaa
     7681 tatttatgat cgtaaacttg attttctaag aaaatattaa aataaaaaga ctagtgtacc
     7741 ttgtttcaag cttaataagc tgaaataagg tatggctagt ctttaacttt aattaaagca
     7801 tcttcaatat ttaaatttta agttaatttt atgtcccaat gctgaataaa taacttcgtt
     7861 ttaat
//
LOCUS       DAKTLH010000119         7832 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.119, whole genome shotgun sequence.
ACCESSION   DAKTLH010000119 DAKTLH010000000
VERSION     DAKTLH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7832
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.119"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            96..782
                     /gene="mtnN"
                     /locus_tag="O0394_002117"
     CDS             96..782
                     /gene="mtnN"
                     /locus_tag="O0394_002117"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCY0500185.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDVIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            802..1329
                     /locus_tag="O0394_002118"
     CDS             802..1329
                     /locus_tag="O0394_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCY0500186.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            1330..2430
                     /gene="yqeH"
                     /locus_tag="O0394_002119"
     CDS             1330..2430
                     /gene="yqeH"
                     /locus_tag="O0394_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCY0500187.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            2444..3250
                     /gene="aroE"
                     /locus_tag="O0394_002120"
     CDS             2444..3250
                     /gene="aroE"
                     /locus_tag="O0394_002120"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCY0500188.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELEGFNITIPHKERIIPYLDYVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            3254..3544
                     /gene="yhbY"
                     /locus_tag="O0394_002121"
     CDS             3254..3544
                     /gene="yhbY"
                     /locus_tag="O0394_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCY0500189.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATHSELVQVIGSMIVIYRESKDNKEIELP"
     gene            3547..4116
                     /gene="nadD"
                     /locus_tag="O0394_002122"
     CDS             3547..4116
                     /gene="nadD"
                     /locus_tag="O0394_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HCY0500190.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHHDFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            4106..4690
                     /gene="yqeK"
                     /locus_tag="O0394_002123"
     CDS             4106..4690
                     /gene="yqeK"
                     /locus_tag="O0394_002123"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCY0500191.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            4691..5044
                     /gene="rsfS"
                     /locus_tag="O0394_002124"
     CDS             4691..5044
                     /gene="rsfS"
                     /locus_tag="O0394_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCY0500192.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYSQVAY"
     gene            5047..5763
                     /locus_tag="O0394_002125"
     CDS             5047..5763
                     /locus_tag="O0394_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0500193.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            5803..6489
                     /locus_tag="O0394_002126"
     CDS             5803..6489
                     /locus_tag="O0394_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HCY0500194.1"
                     /translation="MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYT
                     SRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLKSYFTT"
     gene            6581..7042
                     /locus_tag="O0394_002127"
     CDS             6581..7042
                     /locus_tag="O0394_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCY0500195.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            7047..>7832
                     /locus_tag="O0394_002128"
     CDS             7047..>7832
                     /locus_tag="O0394_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family DNA internalization-related
                     competence protein"
                     /protein_id="HCY0500196.1"
                     /translation="MLFVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPIS
                     LFLIIFSAWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTDKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEVNEQFKERVKEIVIYHLLAVSGSHIAAI
                     VFLIYQPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAII"
BASE COUNT         2856 a          986 c         1499 g         2491 t
ORIGIN      
        1 atacagtaat gaaaatcatg atacttgtcg ttgtagtgat taaaatgtga taaaataaca
       61 aatgctagga ttttttaaaa gaggtgaatc aaaagatgat tggtataatt ggtgccatgg
      121 aagaagaagt aacaatatta aaaaataaat taacacaatt aagcgaaatt tcagttgcac
      181 atgttaaatt ttatactggc attttaaaag atagagaagt agtgattacc caaagtggca
      241 ttggaaaagt taatgctgca atttctacga cattattaat taataagttt aaaccggacg
      301 tcattattaa tacaggttct gctggagctt tagatgaaag tttaaatgta ggtgacgttc
      361 ttataagtga tgatgtaaaa tatcatgatg cagacgcaac agcatttggt tatgaatatg
      421 gacaaatacc acagatgccg gtagcatttc aatcaagtaa acctttaata gaaaaagtat
      481 ctcaagttgt acaacaacaa caattaacag ctaaagtagg cttaattgta agtggtgata
      541 gctttatcgg tagtgttgaa caacgccaaa aaattaaaaa agcatttcca aatgcgatgg
      601 cggttgaaat ggaagcaact gcaattgcac aaacatgtta tcaatttaat gtaccatttg
      661 ttgtagttcg tgcagtttca gacttagcaa atggagaagc ggaaatgagc ttcgaagcat
      721 ttttagaaaa agcagctgta tcatcaagtc aaactgttga agcattagtg tctcaattat
      781 aaaaaggtaa aggtgagtta aatgggttta gttcgcaagt tttttatgcc gaattcatat
      841 gttcaatcaa tatttcaaat tgatttagac aagttagtgg acaaaggcgt taaaggtatt
      901 attacagatt tagataatac gctagtaggt tgggatgtta aagaacctac agaacgtgtt
      961 aaagcatggt ttaaggaagc taatgaaaaa ggaatcacta ttacaatcgt gtctaataat
     1021 aatgagtctc gtgttgctag ttttagtcag catttagaca tcgattttat ttttaaagcg
     1081 agaaagccaa tggggaaagc gtttgataaa gcaataacta agatgaatat cagaccagat
     1141 caaactgttg ttataggtga ccaaatgctt actgatgtat ttggtggtaa tcgtcgaggt
     1201 ctatatacaa ttatggttgt tccagttaaa cgaactgatg gctttattac taagtttaat
     1261 agattaattg aaaggcgatt attacgtcat ttcagtaaaa aaggttatat cacatgggag
     1321 gaaaattgat tgtctgacat tttaaaatgt atcggttgtg gtgcgccact tcaatctgaa
     1381 gataaaaata aacctggttt tgtaccagag cataatatgt ttcgtgatga cgtgatttgc
     1441 agacgttgtt tccgcttgaa aaattataac gaagttcaag atgtaggatt agaaagtgaa
     1501 gactttttaa aattattatc aggacttgcg gataaaaagg gtattgtcgt caatgtcgtg
     1561 gatgtatttg actttgaagg atcatttatt aatgcagtta aacgtattgt cggaaacaaa
     1621 aaaatcattt tagcagcaaa taaattggat ttattgccga aacaaattaa taaacgtcga
     1681 gttaaagaat ggttaaaacg aacagcaaga aaatatggtt tggaagctga cgatgtcgta
     1741 ttaatttcag ctgaaaaagg ctggggcata gacgacttat tatcatcaat tgcgaatatt
     1801 cgagaaaatg aagatgtgta tattgtaggg acaacgaatg ttgggaaatc tacattgatt
     1861 aataaactga ttgaagctag tgttggtgaa aaagatgtag taacaacttc aagattccct
     1921 ggaacaactt tagatatgat agatattcct ttagatgaaa catcatttat gtatgataca
     1981 ccaggtatta ttcaagatca ccaaatgacg catttagtta gtgaaaaaga attgaaaatt
     2041 attatgccta agaaagaaat aaaacaacgc gtatatcaat taaatgaggc gcagacatta
     2101 ttcttcggcg gtctagcgcg catagattat gtatcaggtg gtaaacgtcc gttagtttgt
     2161 ttcttttcta atgacttgaa tatacatcgt actaaaacgg agaaggctaa tgatttatgg
     2221 cgtaatcaac ttggcgattt attaacgcca cctggaaatc cacaaaattt tgatcttaat
     2281 gaggtaaagg ctgttagact tgaaacaggc aaagagaaac gcgatgttat gatctctggt
     2341 ctaggcttta taactatagg accaggggct aaagtaatcg ttcgtgttcc taaaaatgtt
     2401 gatgttgtat taagaaattc tattttataa ggtgattaaa aaaatgaaat ttgcagttat
     2461 aggaaatcct atttcacatt ccttgtcgcc cgttatgcat agagcaaatt ttaattcttt
     2521 aggattagat gatacttatg aagctttaaa tattccaatt gaagattttc atttaattaa
     2581 agaaattatt tcgaaaaaag aattagaagg ctttaatatc acaattcctc ataaagaacg
     2641 tatcataccg tatttagatt atgttgatga acaagcgatt aatgcaggtg cagttaacac
     2701 tgttttgata aaagatggca agtggatagg gtataataca gatggtattg gttatgttaa
     2761 aggattgcac agcgtttatc cagatttaga aaatgcatac attttaattt tgggcgcagg
     2821 tggtgcaagt aaaggtattg cttatgaatt agcaaaattt gtaaagccca aattaactgt
     2881 tgcgaataga acgatggctc gttttgaatc ttggaattta aatataaacc aaatttcatt
     2941 agcagatgct gaaaagtatt tagctgaatt cgatatcgtt attaatacaa caccagcggg
     3001 tatggctgga aataacgaaa gtattattaa tttaaagcat ctttctccca atactttaat
     3061 gagtgatatt gtttatatac cgtataaaac acctatttta gaggaagcag agcgcaaggg
     3121 aaaccatatt tataatggct tagatatgtt tgtttaccag ggtgcggaaa gctttaaaat
     3181 ttggactaat aaagatgctg atattaattc tatgaaaaca gcagttttac aacaattaaa
     3241 aggagaataa catatgctta ctggcaaaca aaaaagatac ttaagaagtt tagcacacaa
     3301 tattgatccg atttttcaaa ttggaaaagg cggtatcaac gaaaatatga ttaaacaaat
     3361 agatgatacg ttagaaaaca gagaattgat taaagtacat gtactacaaa ataactttga
     3421 tgataaaaaa gaattagctg aaacattaag cgaagctact catagtgaat tagtgcaagt
     3481 gattggatct atgatagtga tttatagaga atctaaagat aataaagaaa ttgaattgcc
     3541 ataataatga aaaagatagt actttacggc ggtcagttta accctatcca tactgcacat
     3601 atgatagtag ctagcgaagt atttcatgaa ttacagccag atgaatttta ttttttacct
     3661 agttttatgt ctccattgaa aaagcaccat gattttatag acgttcagca cagattaaca
     3721 atgatacaga tgattatcga cgagcttggt tttggagata tttgtgacga tgaaattaaa
     3781 cgtggtggtc aaagttatac ctatgacacg atcaaggcat tcaaggagca acacaaagac
     3841 agtgagttgt actttgttat tgggacggat cagtataacc aactagagaa atggtatcaa
     3901 attgaatact taaaagaaat ggttactttt gtagttgtaa atcgagacaa aaatagtcaa
     3961 aatgttgaaa atgctatgat tgcaattcag atacctaggg tagatataag ttcgacaatg
     4021 attcgacaaa gagttagtga agggaaatct atccaagttc ttgttcctaa atccgttgaa
     4081 aactatatta agggggaagg attatatgaa cattgaaaaa gcaaaacggc ttgcaaaaga
     4141 gaaactgcca gagaaacgtt ataatcattc tttaagagtt gcagaaacag cgattaaatt
     4201 ggcagaaatt tatgatggag acactagtaa agtagaatta gcaggtgtat tacatgattt
     4261 ctgtaaatat gatgatttag gtaaaatgta tcaaattgtt cgacaatacg aattaggtaa
     4321 tgatctactg agttatggta gtgaaatatt gcatggccct gtgtgtgcag caatcatgga
     4381 acatgaatat ggtatcaatg atgaggaagt attaatggct atcaaatacc atactactgg
     4441 acgtcaacaa atgacaaaaa ctgaaaaact gatttttatt gcagattaca tcgaacctgg
     4501 aagaacaatc ccaggagttg atgatattcg agatatggca tacaatcaag gtagtttaga
     4561 taagacaatt tatgaaattt ctaaacgcac agtactattt ttaatacaga aagatattac
     4621 ggtatataat aagacgattg actgtttaaa ttattataac tatagtgacg aaagaataaa
     4681 ggatgattaa atgaattcac aagaattatt agcaattgct gtggatgcaa ttgacaataa
     4741 aaaaggcgaa gatacgattt ctttagaaat gaaaggtatc agcgatatga cagattattt
     4801 tgttgtaacg cacggaaata atgaacgaca agttcaagcg attgctagag cggtgaaaga
     4861 agtagccaat gaacaaaata tagaagtaaa acgtatggaa ggatacaatg aagcgcgttg
     4921 gatattaatt gacttagctg atgttgtggt acatgttttc cataaagacg aaagaaatta
     4981 ttataatatt gaaaagttat atcaagatgc accattagaa tcatatagtc aggttgcgta
     5041 ttaattatgt cgcaatatgc agaaatgagc ctagtgtacg atcaattgac tcaagatcaa
     5101 ccatatgaaa aatggtttga aattgtaaaa aatcactgca aagatgaatc aaatatttta
     5161 gatattggat gcggtactgg tagtttaaca gttcaattag aagctttagg taatgttact
     5221 ggtatggatt taagtgttga tatgttgact gttgcagctc aaaaatcagc aaatgtaaat
     5281 tggcttgaag gagatatgac atcttttgat ttgcaacaac aatttgatat catcactatt
     5341 ttttgtgact ctctaaatta tttgcaagat gaaactgcag tgattgaaac attcatcaat
     5401 gtttatcatc atctgactga ttcaggtgta tttatttttg atgtacatac tgtttataaa
     5461 atgatgactt tgtttaataa tcaaagttat attgatgata aaggggacat ttttttagca
     5521 tgggatgctg tgcaaggaga tttaccttta agtgtttatc atgatatgac atttttcatt
     5581 cgacatgaag atgaaacata ttcaagattt gatgagtctc attttcagag aacatttgat
     5641 gaaaagacat atttgtcttg gttagctcaa gttggtttta aacatgttga aacatttact
     5701 gattttaata tagatgaaca taatgaagat gcagaaagat tgtttttcat tgcgaaaaaa
     5761 taaaagtaaa accactcgct cagacgtgta taaagttgag gagtggtttt attgtatcaa
     5821 tttttattac gctataaaga ttttttaact caatggaagt tatatattct aagtgctgtt
     5881 gttttaatta tggtattaat tggttttata ttctggagac aagatgatta tacttcaaga
     5941 aattttgaaa ataaagatac tgctctgaaa caaagcacta gtgaaaatag tagtttgtcc
     6001 aaagtagaag atgtccaggt caaagatgga gataattcca aaaataaggg tcctgtatat
     6061 gtcgatgtaa aaggtgctgt taaacatcct aatgtttata aaatgacatc taaggataga
     6121 gtagttgatt tacttgataa agcacaatta ttggaggatg cagatgtaag tcaaattaat
     6181 ttgtctgaaa aattaacaga tcaaaaaatg attttcatac ctcataaagg acaaaagaat
     6241 gttgaaccac aaattgaagt aaacagtgtg cacgaaaaaa atgggaacac aaataatact
     6301 aaagtaaatt taaatacggc atctgtatca gaattgatgt ctgttcctgg agttgggcaa
     6361 gctaaagcta atgcaattgt tgaatatcgc aaccaacaag gtgcatttca agaaattgac
     6421 gatttgaaaa aagtaaaagg ttttggaagt aaaacttttg ataaactgaa atcttatttc
     6481 acgacataat ggattatttt aattgcaaat tttatttttt gtgattaatc ttatattatt
     6541 agaaaatgtt actaaatttt aagtatggaa ggtgaacatc ttggaaagaa tcaaatggga
     6601 agaatatttt atggcacaaa gtcatttgct agcattacgt tcaacttgtc aaagattatc
     6661 tgtaggtgca acgattgtta aggataatcg tattattgct ggtggttata atggctctgt
     6721 agctggcgag gtgcattgta tagatgaagg atgtttaatt gaagatggac attgtatcag
     6781 aacgatacat gcagaaatga atgctttatt acaatgtgca aaacaaggtg tatctactga
     6841 aggtgcaaca atctatgtta ctcattttcc atgcctaaat tgtacaaagt caattattca
     6901 agcaggtata aagcgtatct actatgcaga agattatcat aaccatgaat atgcaactaa
     6961 attactcaaa caatctggta ttgaatttaa aaaaattcca ttttcaccag aatatgttgc
     7021 taaatatctg actaaaggtt aatgttttgc tgtttgtcgc gttatcaatg attgtaggag
     7081 tgctttggaa ttctagcaaa gtgctctcta catttctttt cattttactt ttgtatatta
     7141 cttatcgtaa aaataaaatc gtttatgccc ctatttctct ctttttaatc attttctccg
     7201 catggtattt acattattca caacaagcaa tatttaatta tatcaattat attgaacgta
     7261 attctcagtt taatgagcgt gctcaagtaa tccaaattca acgtcaaggt agtgacacat
     7321 ataaaggtag gttgagttta aaaaatgaaa tatatccttt ctttttaaca gataaaaaga
     7381 attttgattt aaagaaaatt gaaagtcgta attgtattgt taaaggacaa ttcaaagtta
     7441 atgacaataa gtttgtaact cttaaattac aaagtatagt tgtacaaagc tgcctagaat
     7501 cgaaccggtc taatttaatt gagaaacata aacagtttat aatgaatcga atttatgatt
     7561 cgggtattaa gtttccggat cgtattatgg cattgattac tggtgacgta aaagaagtta
     7621 atgagcaatt taaggaacgt gttaaagaga tagttatata tcatttgctg gcagttagtg
     7681 gctcgcatat agctgcaatt gtatttttaa tttaccaacc tttaaaacga ttaaatttac
     7741 ctttatttgt cattaaagga attacaatca ttgtattagc tttatttgct caatacacaa
     7801 attatgcacc tagtgctgta agagctataa ta
//
LOCUS       DAKTLH010000120         7821 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.120, whole genome shotgun sequence.
ACCESSION   DAKTLH010000120 DAKTLH010000000
VERSION     DAKTLH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.120"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..702
                     /locus_tag="O0394_002129"
     CDS             <1..702
                     /locus_tag="O0394_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCY0500197.1"
                     /translation="QLFNKDVHFNTFEVNTSLQSAKNTSVFIPSDVASYQYKNIDEYV
                     ASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQDMLNELPEFEDYVVLTQQQNQNYKIV
                     QQFNNFDKAILLGTSTFFEGFDFQANGIKCVMIAKLPFMNKHNAKYWLMDSEFTSTFK
                     EYVLPDAVTRFRQGLGRLIRNENDRGIIVSFDDRLINSNYKNFFEQTLENYRQKKGDI
                     QQFGKLLRQIQKKKK"
     gene            1024..2316
                     /gene="asnS"
                     /locus_tag="O0394_002130"
     CDS             1024..2316
                     /gene="asnS"
                     /locus_tag="O0394_002130"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCY0500198.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            2644..3330
                     /locus_tag="O0394_002131"
     CDS             2644..3330
                     /locus_tag="O0394_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCY0500199.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKELNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDTAIIQ
                     AALDEANSLNKLSFKYIDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            3320..3979
                     /gene="nth"
                     /locus_tag="O0394_002132"
     CDS             3320..3979
                     /gene="nth"
                     /locus_tag="O0394_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCY0500200.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            3984..4325
                     /locus_tag="O0394_002133"
     CDS             3984..4325
                     /locus_tag="O0394_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500201.1"
                     /translation="MTNITKEVFDNLEQEIDLFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDKLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(4891..7074)
                     /locus_tag="O0394_002134"
     CDS             complement(4891..7074)
                     /locus_tag="O0394_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCY0500202.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(7071..7748)
                     /gene="recU"
                     /locus_tag="O0394_002135"
     CDS             complement(7071..7748)
                     /gene="recU"
                     /locus_tag="O0394_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCY0500203.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNTFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEVFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
BASE COUNT         2712 a         1180 c         1342 g         2587 t
ORIGIN      
        1 cagttattca acaaagatgt tcactttaat acatttgaag ttaacacgtc gttacaaagt
       61 gcaaaaaata caagtgtatt tataccgagt gatgtagcat cttatcaata taaaaatata
      121 gatgagtatg tagcatcgat tgtaagctat attattgaat atacgactat aacgtcatca
      181 aaatgtttag tcttatttac gagttataaa atgatgcata tggtacaaga tatgctaaat
      241 gaattgccag aatttgaaga ttatgttgta ttaacacaac agcagaacca aaattataaa
      301 atagtacaac agtttaataa ttttgataag gccatactgc ttggtacgtc aacatttttt
      361 gaaggttttg attttcaagc aaatggtatt aaatgtgtga tgatagcgaa gttaccgttt
      421 atgaacaagc ataatgcaaa atattggctt atggattctg aattcacttc aactttcaag
      481 gaatatgtat tacctgatgc agttacacgt tttagacaag gacttggaag attaattaga
      541 aatgaaaatg atcgcggcat aatagtttca tttgatgatc gactcataaa cagtaattat
      601 aaaaactttt ttgaacaaac acttgaaaat tatcgtcaga aaaaaggcga cattcaacag
      661 tttggaaaat tattaagaca aattcaaaaa aagaaaaagt gaaatacaaa gtaaaattct
      721 tgtgacatga catgtgctat gttctgttac aagaattttt gtttggtcgt caaaatttga
      781 gttttataat taaacttgaa tgttagcaca aaaaacgacc ttcatactaa taatattgag
      841 gctttattat atattttgtc tgaaactgat gattgtattg tcttaatata aattaatgtt
      901 caaatctgtg cgtaaatcat taccatttac acgttatctt ttagtctagt tctagaactt
      961 gaaagcggat tttgttaaaa tgaatgagta ttgtataacg aaaaaataaa taggagaatg
     1021 gttatgaaaa caacgattaa acaagcgaaa gatcatttaa accaagacgt tacaattggt
     1081 gcttggttaa caaataaacg ttcaagtggt aaaatcgcct ttttacaatt acgtgatgga
     1141 acaggcttta tgcaaggcgt agtagttaaa tcagaagttg atgaagaggt attcaaactt
     1201 gcgaaagaaa ttactcaaga atcatctcta tacgttacag gcacaattac agaagataat
     1261 cgttctgact taggatacga aatgcaagtg aaatcaattg aagttatttc agaagcgcat
     1321 gactatccga ttacacctaa aaatcatggt acagaattct taatggatca ccgtcattta
     1381 tggttacgtt ctaaaaaaca acatgctgta atgaaaatta gaaatgaagt tattcgtgca
     1441 acgtatgaat ttttcaacaa agatggattt acaaaggttg atccaccaat tttgacagca
     1501 agtgcgccag aaggtacaag tgaattattc catactaaat actttgatca agatgcgttt
     1561 ttatctcaaa gtggtcagtt atacttagaa gctgcagcaa tggcacacgg aaaagtattt
     1621 tcatttggtc caactttcag agctgaaaaa tcaaaaacac gtagacactt gatcgagttc
     1681 tggatgattg aaggagaaat ggctttcaca aatcatgctg aaagtttaga aattcaagaa
     1741 caatatgtaa cacatgttgt aaaatcagtt ttagaaaatt gtaaactaga gttgaaaatt
     1801 ttagagcgtg atacatcaaa acttgaaaaa gttgcgacac cattccctag aatttcatat
     1861 gatgatgcaa ttgaattctt aaaagcagaa ggctttgatg atattgaatg gggcgaagat
     1921 tttggtgcgc cacatgaaac agccattgct aatcattatg atttaccggt gtttattact
     1981 aattatccaa ctaaaattaa gcctttctat atgcaaccaa atcctgaaaa tgaagaaact
     2041 gtattatgtg cagacttaat tgcacctgaa ggatacggtg aaattattgg tggatctgaa
     2101 cgtgtggatg acttagaatt gttagaacaa cgcgttaaag aacatggatt agacgaagaa
     2161 gcatatagtt actacttaga cttacgtcgt tatggtagtg tgccacactg tggatttggt
     2221 ttaggtttag agcgtacagt agcatggatt tctggtgttg aacacgttcg tgaaacagcg
     2281 ccattcccaa gattattaaa ccgtttatat ccataagttt tagggtcgtt acttaaaata
     2341 tatattatga taataaaaag aatcgtccag cctatattaa tagctgggcg atttattctt
     2401 ggaaaatgaa gattgataac ggcttatatt aatcatgaac aaacttttgt ttagtaacta
     2461 tatttggaaa ataaatagtt catctgatta tccataaagt tcattgttaa aatctagtgt
     2521 taaaaaatac tgttatttaa aatttgtttg ctactagtta aatatattaa gcattttagt
     2581 cgaaaattaa agaaaatgat ttatactatt aagtaatggt taaatttaaa agggggcacg
     2641 aacatggata aatatcaatt aaaagcaaga cctgtagtga tacgaagaga attattagac
     2701 cattacagtg acttaggttt agatgagcaa gatttagtca ttttgcttaa attgatttat
     2761 gcttccgaaa catcaaataa acagccttca attgaattgc ttcagaaggg ctcaactatg
     2821 caaccacgtg atattacaat ggttattcaa aatttgatac aacgtgaatt gctagaatta
     2881 caagttcaaa aagatgaaga aggtagattt accgaatata tgaatctaga tccttttttc
     2941 gaaaaattaa gtcatatatt gaagcaacaa agtatggaaa cgaaagagct aaatagtaaa
     3001 gaaaaattta aacaattatt tagagtttta gaggatacat ttgcaaggcc gttatctcca
     3061 tatgaaatag aaacgttaaa tcaatggatt gatgttgata aacacgatac tgcaattata
     3121 caagcggcat tagacgaggc gaatagtttg aataaactta gctttaaata tatagatcgt
     3181 attttactta attggaagaa aaacaatgtc aaaacaattg acgattctag aaaaataaga
     3241 gaaaaattca ataaaccgaa aatgacgcac acagttaaaa cggtacctaa atttgattgg
     3301 ttaaacgggg agaaccttga tggtaagtaa gaaaaaagca ttagaaatga ttgatgttat
     3361 agcaaatatg tttccggatg cagaatgtga attaaaacat gataatccgt tcgaattaac
     3421 tattgctgta ttattgtcag cgcaatgtac agacgttcta gtaaatagag taacaactga
     3481 gctgtttaaa aaatacaaaa cgcctgaaga ttatttggca gtcagtgatg aagaacttat
     3541 gaatgatatt cgttctatcg gtctgtatcg taacaaagct aagaatatta agaaactttg
     3601 tcaatctttg attgatcaat ttaatggaga aataccacaa acacataagg aattagaaag
     3661 tttagcaggt gtaggacgta aaactgctaa tgtagtcatg agtgtagcat ttgatgaacc
     3721 ttcattagct gttgatacgc atgtagaacg tgtttctaaa cgcttaggta ttaatcgttg
     3781 gaaagataat gtgagacagg tagaagaccg tttatgttct gtcataccta gagatagatg
     3841 gaataggagc catcatcaac tcattttctt tggaagatac cactgtttag cccgaaaacc
     3901 taaatgcgat atttgtccac tattagaaga ttgtagagaa ggacaaaaac gttataaagc
     3961 tagtttgaaa gaagcgtgat aacatgacaa atattacaaa agaggtattt gataatttag
     4021 aacaagagat tgatttattt gccaaaaata agacattagg ttctagtgaa gcaaagccct
     4081 acttggatga ataccatagt aaaattattg attattttaa gcaggttaat gacataactg
     4141 gcaatattga ttttgataaa ttaaatcaat atcctgttgt gccaatgaat tttaaagaaa
     4201 gatatgatta tatgattgaa cgtaaatatc attttatggg ctatcgacaa atgaagacct
     4261 ttaaaactga attgattaaa atgaatgcta gttatcaaac aaggttaaag aataagcagg
     4321 tatagacaag attgatcatt tcctaatgtt gaaaatatat aaaactaatt catgcacaca
     4381 tatttataat tatgttaggt gtcgtgtcat tacaacgtct taatataaaa actgtccatc
     4441 tacatcataa tggatatctt gagatatgct ttaagatatc tatttcgaat cgtaggtgga
     4501 tttcttatat ctatgattgt gaaaatatgt tgcaaacatg ttgatatttc actgattaac
     4561 gtacacaggg aattaataaa tatatcaatt taaaacagcg tctaagatga ctatgagaaa
     4621 atataatcat aattttaaaa atgggaacaa ccacttattc aaattcaata attttacata
     4681 gctaaagatg tgaatatgaa aaactattta gatgtttcaa aatgtatgtg aatgactgat
     4741 tttacggttt ctactaatat agcaatagcg tatgaatact tataaaaaaa gcaggaagtt
     4801 ttaccttccc accataaaag atgaagaacc taaaaaaatc ctcggctaca ccataaatat
     4861 ggtataactg aggattttat gttgagtgga ttagttgaat atacctgtta atccaccgct
     4921 gtttctagat tgttgagatc tagtattgtt atttgattgt gcagtaccac tgctgtttgc
     4981 tgatgtgtca ctaccaccgt gtgtactacg atttgtagtg ttgttatctt gactaccaga
     5041 aacattgatt gatgggatac taccacttac tgagctagga cgtttaaagt cttcgccatc
     5101 tctagatgaa atttttgaca tcacattttc atataagaac tgtggatatt cttgttggct
     5161 atgtcccaca aatgagtttt caccatattg tttaacttta ctgaagccca tccacactga
     5221 catagtgtat tgaggtgtaa agccgttaat ccacacgtct ttcgctgcat tatcaggtaa
     5281 attatattgt gaataagttt cagcaccgta agtaccagta cctgtcttag cacccatatt
     5341 tactccagat acaccatggc catatgcaga accatatggt ttaaatgtac cttttagcat
     5401 ctcagctaac atgtatgcag tgtaatcact catcgcttta tggctagtat gatcgtattc
     5461 gattgtttca ccatcacgag taactacttt ttgaattgaa tgcgcgttgt tataagtacc
     5521 accgttagcg attgcagcaa atgctgatgc taattgtgtt ggtgagaatt ctgaagcaga
     5581 accaccaagt acttcagatg gaccaatatc gccttcgtag tttaagccaa gtttggcagc
     5641 gaatttctta ggtgcatcat taccagcatt ttgcttaact gattgccaag cttttaaagc
     5701 tgggatattg aaactttgtc gtaaagcatc ataaatagat acagtaccgt gactcttcgt
     5761 atcatagttt ctaaatgtag aaccatcaac ttgatatgaa gattcatctt gaatcgcatg
     5821 gtttgttgcc catttcatat tttcaatggc aggtccatac gctaagaaag gttttaaaga
     5881 tgaaccagta gggtgaggat ctgttgcttg gtttctgtta acgacgtctt tgaaatcacg
     5941 tccaccagat atagcaacta aaccaccagt tttactatca agaattgttg caccaacttg
     6001 ttggtcttta ttcttgtaga agctaccatt atcaacatca ttttgtaatg ttttttgaac
     6061 atctttatcc atgtttgtat aaattttaat accactttgt aatacattac ctaaattttc
     6121 atctttgaat gctttattat tcattaattc agattttaca aagttaacgt atgaattata
     6181 ctcagaatct tgatttgtat caatgttttg acgttcttca ggagtacgat ttactaagtt
     6241 cgcttttaaa tcgattttct tagcatcttc ccactgttta tctgtaatgc gtttatgata
     6301 atgcattaag tataaaacag tgtttttacg atcttcagca gcttttggat gatcataaat
     6361 attatagttg tttggaacct gaggtaaacc ggctaaataa gcttcttccg ctaagtttaa
     6421 atcttttaaa tctttattaa agtaatactt agcagcagct ttaatacctg ttacgccatc
     6481 agaatagtaa attttgttta gatatacttg gaagatatca tctttactat actcttgttc
     6541 taaacgatat gataagtatg cttcttgagc tttacgtcca atagatttat gttgtgataa
     6601 aaatgcatct ttaacaactt gttgtgttaa tgttgaggca ccttcagaac caaatccacc
     6661 agtcaagttc ttaccaattg caccgaataa acgtttataa tcaagtgcgc catgttcgta
     6721 gaaacgattg tcttcagttg caagtactgc gtctttcatt gatttcggca cgtcttttaa
     6781 atttacatgc tcatgtcttt ggccattatc taatgtttta acaagttctc cgttcttgtc
     6841 atatatcttt gcaggaatcg gatcttgtaa tttagcttcg gtaaaagcag gtgctttcca
     6901 agcataataa gcaaacaata agatacctag taaaagaaca acgaaaaatg caataatcat
     6961 gaagccaata atcttaataa tcgttctctt cacatttcta ttcttttttg agttggattt
     7021 cccaccatta ttaccgtttt tcttaggctg agaagatcct ttgttttccg tcatacgcgg
     7081 tcctcacttt catctaatat caacttatca actgctttta gatagtctaa tcttggttga
     7141 tactgataag gaatatggta accatttttt cgtatttcat caactgttat agacttttta
     7201 atattatctt tatatctctt ccaaaatact tcgaattttg aatagggtaa aagataaact
     7261 tcatctagcg ttttaaaacg aatcattaaa aacacaatac ctttttgttg atatgcattt
     7321 ttcatatgtt cgacttgatg gtcatgaata ttatttaaag gaaaggacgt cttgttttta
     7381 gtttcctttg cttcaaaatc aatataataa ccttgataaa cgccgttgta atcagttgtt
     7441 gaaggtgtac gaaaataagc ttcgttaatc acagctttac tccgcttagg atagttgaca
     7501 ttaactattt gtactggcgt aggctttttg tgaataactg caatgtcgct tttaagataa
     7561 aacgtatttg aatgttcgat atctttttca agtgacatac cacgtccacc atactcaata
     7621 ttactaaagg cagcggtctt tttccctccg tctatagcac tatttttacg atatggttta
     7681 ccatttggat aattcataaa tttcaccaca caagtttggt agtaaacgtt gtttattata
     7741 cacaacatta tagtgaaaca ccaaacttaa attagtataa atgcgaaaga cacaatacac
     7801 caaagcttat tttaacgtga t
//
LOCUS       DAKTLH010000121         7808 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.121, whole genome shotgun sequence.
ACCESSION   DAKTLH010000121 DAKTLH010000000
VERSION     DAKTLH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.121"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..1121)
                     /locus_tag="O0394_002136"
     CDS             complement(<1..1121)
                     /locus_tag="O0394_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1181 family transposase"
                     /protein_id="HCY0500204.1"
                     /translation="MCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPS
                     ACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIV
                     DESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLP
                     EHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVK
                     TVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPDYT
                     IFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRHSYELVQGLR
                     KDLRLCNWPKFINRLNSVSKKSVSKGVWKAVKYYRKHQR"
     gene            1614..2675
                     /locus_tag="O0394_002137"
     CDS             1614..2675
                     /locus_tag="O0394_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCY0500205.1"
                     /translation="MSNANNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKFEVPKQ"
     gene            2933..4390
                     /locus_tag="O0394_002138"
     CDS             2933..4390
                     /locus_tag="O0394_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCY0500206.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            4383..5105
                     /locus_tag="O0394_002139"
     CDS             4383..5105
                     /locus_tag="O0394_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCY0500207.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            5256..6383
                     /gene="queG"
                     /locus_tag="O0394_002140"
     CDS             5256..6383
                     /gene="queG"
                     /locus_tag="O0394_002140"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCY0500208.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPLIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            6388..6858
                     /gene="trmL"
                     /locus_tag="O0394_002141"
     CDS             6388..6858
                     /gene="trmL"
                     /locus_tag="O0394_002141"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCY0500209.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEVTNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            7017..7616
                     /locus_tag="O0394_002142"
     CDS             7017..7616
                     /locus_tag="O0394_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HCY0500210.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            7641..7793
                     /locus_tag="O0394_002143"
     CDS             7641..7793
                     /locus_tag="O0394_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500211.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
BASE COUNT         2738 a         1036 c         1419 g         2615 t
ORIGIN      
        1 atcctttgat gttttctata atatttaacc gctttccata cacccttact cacagacttt
       61 ttactaactg aatttaaacg attaataaat ttaggccaat tacataacct taggtctttt
      121 cgtaatcctt ggacaagttc gtaggagtgt cgtagtatat cgtcttttga aagcatgaat
      181 tctacaatgt cagatgagcg tttataagcc ttaaaagatt tattccatct gtatttacta
      241 aatatggttt tactagtatc catcaatagg actttccagt tattcttaaa aattgtataa
      301 tcaggtcctt ttttattacg gtattcattc ataacttgta cacgatactt attaagttct
      361 ctatttaaat gttgaacgat atggaatctg tcaaaaataa tagctgcatt aggaaatagg
      421 tcgcgaaata atcggacata gggttcatac atgtcaatag taaccgtctt aacttgttgt
      481 cgatttttta aatcgaatcg ctcgaaatag gcacgcaaga atcttgtagt tctattttct
      541 aaaatatcta taacatcatg agtatcatta tctataaaaa tgaaactcat tgatccagtt
      601 acatttttaa cgcttttaaa ttcatccata gcgatgtgtt ctggcaaaca attaaagggt
      661 ttaattcgta atgaattagc cgctttagtt ctaatacgac ttacagtaga tggagagata
      721 cagttatctt ctgcagtatc aatctctgtt ttaactttag tagcttcttc tataactttt
      781 tgagtaacac atcttgagat aaaacacgac tcatcaacaa ttgacgtttt agcagtaaaa
      841 gtgccattgc attctaagca tttaaaacgt tgtttagcta agttgaggta tgtgttatac
      901 ccttgagttt taagtaaagt aatacgagaa acacgcttac catgtttatg aatttgtcct
      961 tcatttttaa ctccacaaca ataacaggcc gaaggcttat acgttagtac agcattaacc
     1021 actgttgctt tttttccttt gataatgacg tcaatttctt ggtttatata ttttatattt
     1081 tcatctttta ttcttagtaa ttctaaggta tcattacaca taggcgcact tgtctccttt
     1141 agtttgggtt tggtcacttt aaattataga ggcaattgcg ctttttgtgt attaaaaaaa
     1201 gcaggaagtt ttaccttccc accataaaag atgaagaacc tgtttaactt ctttttttgg
     1261 tattacatta atgataatga ttatattatc tggggttata tatttgtttc aacttttcta
     1321 ataatatatt tgtaaaagtg ctattttatt gtgctttata ccaagtcgat tcaatttatc
     1381 tatcttaata gtaatatggc tattttaata tttacaattt attacattat tagtcacagg
     1441 atgtaattag tagtatttta aaattaacat gacattgatt agtgagtaga ttgtttaaat
     1501 ttgctttatt caatttgtgg attgttatat ttagattaaa ttagccaact aatcattgtt
     1561 aaagttaatt ttctataata tgatgagttt aatgttcaaa ttggaggtcg tttatgagta
     1621 acgcaaataa tgctgataat aaacagtttt tcagtaagat tttaaattct attggtgctg
     1681 gtgttgttat tgcacttgtt ccgaatgctt tattaggtga agtattaaaa atatttaaaa
     1741 gtggtaatga aattttagaa ttaacgtatc agttagtgat actaattcaa tcatttatgg
     1801 catttattat tggggtttta gctgctcatc aatttaaatt taatggtgca ggtgctgcta
     1861 tagtaggtac atcagctatg attggttcag gtgctgttgt atatagcaat aatagtttta
     1921 tgttaaaagg tataggtgac attataaata cgagtttggt tgttattatt gcatgtctta
     1981 tatatatggt attacaaaac aaacttggat cttttgaatt aattatatta ccagttttag
     2041 tacctatcgt tagtgggggt atcggtctaa tcacattacc ttacattcga aaaattactc
     2101 aagcaattgg aaatgtaatt cattctttta cagacttaaa tccgttgtta atgtctatac
     2161 ttattagtgt ggcattttca ttattaatgg taacaccaat atcgttagta gcgattgcaa
     2221 ctgcgattag tttaaacgga ctaggaagtg gagcagcaaa cctaggtata gtggctgcct
     2281 gtgttacttt cttatttggc tcattgcgtg tcaattctat tggtgtaaat gcagttttac
     2341 tcataggagc tgcaaaaatg atgatcccag tttacttgaa aaatttaatt atttcgatac
     2401 cattgactat aaatggaata attactggaa tcattgctta cgttcttcag gttaaaggaa
     2461 caccattgtc tgctggattt ggatatacag gattagttgg cccaataaat gcatttaacc
     2521 gaatgtcagg tgatccaaca atgaatatta ttttattagc acttggttat tttgtcatac
     2581 catttgtatc ggcatttatt gttcatgaat tgtgtaaaaa attcattcct atatattcta
     2641 atgatattta taaatttgaa gttccaaaac aataatttat agcttgatga caagaataag
     2701 ttttaatata tgataaatat aggcgttact gtcaataaga tattgatagt agcgcttttt
     2761 tgattgattt taaaatgtga gatttgttca cgtataatag cggatttttt atgtacagtc
     2821 ttttaaaaag aagcttttct ttatattgat aaaaatacat ttataaaaca ctattgcatc
     2881 aaaatgtatt aatatgtata attatgaata ttaattaggg agggctgtat aaatgaagtg
     2941 tttaattagg tttatattag tattagggtt gttaattagc agtgctatgg tgtatatcaa
     3001 tcccacagca catgctgaac aagatcaaac atgggagaag attaaagaac gcggtgaact
     3061 tagagtgggt ctttctgcag attatgcacc aatggaattt gagcatacag ttaatggtaa
     3121 gactgagtat gcaggtgtag atattgattt agctaaaaaa attgcgaaag ataataattt
     3181 aaaattaaaa atcgtcaata tgtcatttga tagtttgtta ggagctctta aaactggaaa
     3241 aattgatatt attatttccg gaatgacttc aacgcctgaa cgtaagaagc aagttgattt
     3301 ttcagattca tatatgatga ctaaaaatat catgcttgta aagaaagata aagttaatga
     3361 atataaagat atcaaagact ttaataataa aaaagtaggg gcacaaaagg gaactgaaca
     3421 agaaaaaatc gctcaaaccg aaattgaaaa tgcatctatt acttcattaa gccgtttacc
     3481 agacgttata ttagcattga aaagtggaaa ggttgaaggt gctgtagttg aaaaacctgt
     3541 agcagaagca tatttaaaac aaaatcctaa attaggaatt tccaatgtga aatttaatga
     3601 agaagaaaaa gatacagtga tagcagtgcc aaaagattca ccaaaattat tgtcacaaat
     3661 taataaaacg attaaggagg ttaaagataa aggattaatc gataaatata tgactaatgc
     3721 tgcaaatgcg atgaatgatg acagtggttt tatttctaag tatggtagtt ttttcttgaa
     3781 aggaattaag attacaatat taatttcact tatcggtgtt gcattaggtt ctattttagg
     3841 tgcattcgtt gcgttaatga aattaagtaa aataaaaatt atttcatgga ttgcttctat
     3901 ctatatcgaa atattaagag gaacaccaat gttagttcaa gtatttatcg tattctttgg
     3961 tataactgct gcattaggac tagacatttc agctcttgtt tgtggaacaa ttgcattagt
     4021 tattaattcc tcagcttaca ttgctgaaat tattcgtgca ggtataaatg ctgttgataa
     4081 aggccaaatg gaagccgcac gtagtctagg tttgaattat agacaaacga tgaaaagtgt
     4141 aattatgcca caagcaatta aaaatatttt accagcttta ggtaatgaat ttgtcacttt
     4201 aattaaagaa tcatctattg tgtcaacaat tggagttggc gaaattatgt ttaatgcaca
     4261 agtggttcaa ggtatttcat ttgacccatt cacaccatta atagtggcag cagcattata
     4321 ctttgtctta acatttgtac ttacacgcat tatgaacatg attgaaggga gattgaatgc
     4381 cagtgattaa aataaacaat cttaataaag tttttggaga taatgaagtt ttaaaagata
     4441 tcaatcttga aatcaatcaa ggggaagtag tagcaataat aggtccatct ggtagtggta
     4501 aaagtacatt gttaagatgt atgaatttat tagaagtacc tactaaaggt caagtgattt
     4561 ttgaaggcaa tgacttaacg gaaaaaggga cacaagtaga taaactacgt caaaaaatgg
     4621 gtatggtatt tcaaaacttc aacctatttc cacataaaaa agttgtcgat aatattattt
     4681 tagctcctaa attattaaag aaagataata acgatgaatt acataaggaa gcattgtcgt
     4741 tattagataa agtgggatta aaagaaaaag cagatgtata tccgaatcaa ttatcaggtg
     4801 gtcaaaaaca aagggtagca attgcaagag ctttagcaat gcatccagat gttattttat
     4861 tcgatgaacc aacttcagca ttagatcctg aggtagttgg tgatgtatta aaagtaatga
     4921 aagacctagc caaagaaggt atgaccatgg tggttgtgac acatgaaatg ggatttgcca
     4981 aagatgtaag tgacaaagtc atatttatgg cagatggcgt tgtcgtagag tcaggcacac
     5041 cagtcgaaat atttgaacaa ccgcaacatg aaagaacaca aaatttctta gcaagagtat
     5101 tataacaacc taacgaagct tgaatatatg atacgcacca caaagttata tcatatattc
     5161 aagcttttta ttattaaaat tatgtgaaat gaaacctcaa tatgaataat ggttgtgtta
     5221 aaataacata gtattatttt atggggggca tacgattgga tacaaagcaa ttaaagcaag
     5281 atatcattga ctatgcatat acaattggta tcgacagtat tggatttact actgccgatc
     5341 cctttgatga attgaagcaa aagctagaag catatcatgc aaatggttat gcctcaggat
     5401 ttgaagaatc tgatattgct ttacgaacgg agcctaaatt atccttacca acagcaaggt
     5461 caatcatagc aattgcagtt ggttatccta acaaactgaa aggtgcacct aagagtgtta
     5521 gaggtgatcg cagaggctta tttgctagag catcgtgggg tcaagattat catacaatta
     5581 tgcgtaaacg attagacatg ttagctgcat ttattgaatc taaagttcca gatgttgaaa
     5641 tcaaatctat ggtagatacg ggtgtattat cagatagggc agtagcagaa cgtgcaggtt
     5701 taggatttgt tggtcgtaat ggctttgtca tcaatcctaa actaggaaca tggacatacc
     5761 tcggtgaaat gttagtcagt ataccttttg aacctgatga tccattatta gatagctgtg
     5821 gtgattgtac aatttgtgtt gatcgttgtc caacaagtgc attagttgga aatggccaat
     5881 taaatagtca gaaatgcatt agttttttaa cgcaaacaaa aggctatatg cctgaccaat
     5941 atcgttataa aattgggaat agattatatg gttgcgatac gtgtcaacaa gtttgtccga
     6001 aaaatagagg tattaatacc gaacaagatg acatcatttt ggaaccagaa attttaaagc
     6061 caagattagt acctttacta cgtatgtcta ataaagaatt caaacaaaca tatggtcacc
     6121 ttgcaggtgc ttggcgtggt aaaaagccga tacaacgaaa tgctatttta gcattggcgc
     6181 attttaatga agtggatgca attccagaat tgaaaaaagt tgcaacaaca gatgaaagac
     6241 cgttgattcg agcaactgct tattgggcca ttggtcaaat tcttggggaa gaagcaagag
     6301 attttatcaa tgctaattat gatcaagagg atgcagaagt tcagaatgaa atgataaaag
     6361 gattagacac aaggagagaa tagtaatatg acaaatcata tcgttttata tcaaccagaa
     6421 atcccagcta atactggtaa cattgcacgt acgtgtgcag gtacaaacac acatttacat
     6481 ttaattaaac ctcttggatt tagaactgat gataaaatgt taaaaagagc cggtttagat
     6541 tattgggaat ttgtgaatat tacgtatcat gatagtattg aagcattttt tgaagtcaca
     6601 aatggcgagt attacttatt aacaaaattt ggtaaaaaga cttatagtga ttttgatttt
     6661 tcaaatcatg acaaagattt ttacttcatt tttggaaaag aaacgacagg attaccagac
     6721 tgggtgaaag agaaatatca agacacagcg ttaagaattc ctatgagtga acatattcgt
     6781 tcattaaatt tatcaaatac tgctgcgttg ttaatttatg aagcgttacg tcaacaagat
     6841 tttccgggat tgaattaaaa tttactcatt ttcattataa aaatgatgct taagtgctat
     6901 atttagagta taagcatgtt aaagttaaat ggtttgatat attgaaagtt agggtataat
     6961 aattattaat atcgccgttt ttatgtaaaa tgcaaactaa gaggagtgta gaaatcatgg
     7021 caatgaactt taaagtcttt gacaatagtc aacttgtagc agaatatgct gctgatatta
     7081 ttagaaagca atttaacaat aatcctacta caattgcagg ttttcattta gatacagatc
     7141 aagcgccagt tctagatgaa ttaaagaaaa atgttgaaaa acatgctgtt gattttagcc
     7201 aaataaatat tttagattat gacgataaaa aatcatattt cgaagcgtta ggtgtaccag
     7261 caggtcaagt ttatccaatt gcttatgaaa aagatgcaat cgaattaatc gctgataaga
     7321 ttaaaactaa agaaaataaa gggaaattaa cattacaagt tgtttctatc gatgagcaag
     7381 gtaagttaaa tgttagtatt cgtcaaggac taatggaagc aagagaaatt ttcttagtag
     7441 tgacaggtgc taataaacga gatgtagttg aaaaattata tcaagaaaat ggtaaaacaa
     7501 gcttcgaacc agccgattta aaagcacata gaatggtaaa tgttattctt gataaagaag
     7561 cggctgcagg tttacctgaa gatgttaaag cttactttac gtcacgcttt gcttaattaa
     7621 aaatctgtga ggtattgatt atgtctaaag acaaagatcc aaaattaaat tatcatgaag
     7681 aagaaaacag tatggtaacg gattttgaag atttaaaaga attaggtaaa gaaatggaac
     7741 aaatctctga tcaaaatgat caagaaaaga attctgaaga agacagtcag taaagtatta
     7801 taattgtg
//
LOCUS       DAKTLH010000122         7789 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.122, whole genome shotgun sequence.
ACCESSION   DAKTLH010000122 DAKTLH010000000
VERSION     DAKTLH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.122"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            167..568
                     /locus_tag="O0394_002144"
     CDS             167..568
                     /locus_tag="O0394_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0500212.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(831..2528)
                     /locus_tag="O0394_002145"
     CDS             complement(831..2528)
                     /locus_tag="O0394_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HCY0500213.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFITS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLYKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQS"
     gene            2803..3426
                     /gene="gmk"
                     /locus_tag="O0394_002146"
     CDS             2803..3426
                     /gene="gmk"
                     /locus_tag="O0394_002146"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCY0500214.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLEHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            3426..3644
                     /locus_tag="O0394_002147"
     CDS             3426..3644
                     /locus_tag="O0394_002147"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCY0500215.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            3860..5059
                     /gene="coaBC"
                     /locus_tag="O0394_002148"
     CDS             3860..5059
                     /gene="coaBC"
                     /locus_tag="O0394_002148"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCY0500216.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            5059..7467
                     /gene="priA"
                     /locus_tag="O0394_002149"
     CDS             5059..7467
                     /gene="priA"
                     /locus_tag="O0394_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCY0500217.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMVASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
BASE COUNT         2708 a         1127 c         1430 g         2524 t
ORIGIN      
        1 atcatatttt taatcttgcg taatttttat cgttaactat gtgatttaat caacaggcca
       61 ataagacgtt tgtcattcgt gcacataacg gtacgacgcg cttttgtcat tttaattatg
      121 ttaagataat agtagttata gaagttcaat ctataggagg catagcatgg atattccaaa
      181 aatcacgaca tttttaatgt ttaataacca agctgaagaa gctgttaaac tatacacaag
      241 cttatttgaa gatagtgaga ttataacaat ggctaagtat ggtgaaaatg gacctggtga
      301 tcccgggact gtacaacact caatatttac attaaatgga caagtattca tggcgattga
      361 tgctaatagt ggcacagaat taccaatgaa tcctgcgatt tcattatttg ttacagtaaa
      421 agatactatt gaaatggaac gactatttaa tggattaaaa gatgaaggtg ccattttaat
      481 gccaaaaacg aatatgccac catacagaga gtttgcttgg gttcaagata agtttggagt
      541 aagttttcaa ttagcattac ctgagtaaaa ggattcgcac agcgttgaat gataaagaaa
      601 cactttttct tatgcatgcg ttttacctat gtatttctat tttaagtaca cattagcatt
      661 ttagtttcac tcatttttaa aatcacaaag ataatttgta acttaaatga tgatacgtta
      721 tttaaaaaca cgatacttcg tttcaatgaa cgcattaaat aataaataaa cacctcacca
      781 taagaaagga ctactttctt ttgtgaggtg tttatttgtc gacaacttga ttatgattgt
      841 ttcatttttt gaataagttc ataatcaggt gtagcataca aagttttttg attgtcatat
      901 gttacaaacc caggctttgc acctgatggt ttatgcacat ttttaattaa tgtataatca
      961 acaggtattt gtccagaatt accagctttt gaaaagtatc ctgctaacat agccgcttcc
     1021 ttgattgtcg tatcacttgg tgcatcatta aatataacga catgtgaacc aggaatatct
     1081 tttgtgtgta accatgtgtg agttttttta gcttttttat ttgttaaata atcattttgc
     1141 ttgttattct taccaacata tatatcgtcg ccatcagttg atacataatg ttgtaattga
     1201 atctgcgctt tctttttctt agtttgattt ttacgctgtt tcataaagcc ttgttctgct
     1261 aattcatctc taatttcatc aatgtcatgg acagaaatat gatgtaattg ttgttcgatt
     1321 gttgaaaaat aatctatatt gtctttcgtc aattgaattt gatgttgtaa ttcacgttct
     1381 ctcgttttca tacgattata ttgtttataa taatattgag catttgctga tggggatttt
     1441 gtaggattta aaggaatgac aacttcttca ttcgtataat aattcaatgc cgtcacttct
     1501 ttatcgcctt gcttaattcg atatatatta gcagtgatca attcaccata taactgttca
     1561 gtatctttat ttttagactg ctcatattct tcaatcaact tcgctaattt attttgatat
     1621 ttgtacaact gctgttgaac aaatcgaact aaatcattcg cacgttgttt aacgcgttca
     1681 cgttcgccac gcgcatcata aaaacgatca agcaaatcat ttaatgaatc gtatgtaact
     1741 gtatcatcat taaattgatt taactttata aaatagaaat cctctttacc tgtttcatga
     1801 tttttatgaa aaataggagt aggtggtaac ttggtttctg ccattacttc gtcaaatgct
     1861 tctggtaatg ttgatgaagt aataaattga cgacgactaa cgatttcatt cgtaattaaa
     1921 gggctaaatc cttcaaactg attcaataat tgtttagcaa tattacctgc gttaaaatcg
     1981 atatatttca acacctctgc acctgtaata tcatacggat ttattttgtg ctgagtaggt
     2041 ggtgcttcat aattaaatcc tggcattact gtacgatagt gattcgtatt tggtgttaag
     2101 tgtttaaatc cttcaattat tttgcgattt tcatctacta aaattaagtt actatgttta
     2161 cccataatct caaggatgac agtgcggtaa atagtatcgc caatttcatc tttactcttt
     2221 atatcgattt caatgcgacg atcattacca atttgcttaa tcgattcgat aataccacct
     2281 tctaagtgtt ttctaaaaac acgcgcaaac atgggtggat taaatggatt atcatatttt
     2341 ttagtagtca attgtaatct tgaaaagttt ggatggattg acaataacaa ttgatggttt
     2401 tgtctatttt gacgtacaac cattagtatc gtgtcattat caggttgatt gattttgtga
     2461 acgcgtcctg ttgttaaaaa ttgtagagac tcaaccattt tctttgtaaa taagccatca
     2521 taagccataa tttatcttcc accttcttca tatcattcgt ttcattttaa catgattgta
     2581 acttttcagt atagttattg aaccatacat tatgccatac attttacatt atctaaaagt
     2641 ttgcaattca atttatttaa ttaaaattaa tcgtgcaata taacgatatt gttagactta
     2701 atagaaatta tggcatgcaa tttcaaatat gctatacaat ataaagaaca atgtgatatc
     2761 atatttaaat aatagaagat tagcttagag aggtcgtaag gcatggataa tgaaaaagga
     2821 ttgttaatcg ttttatcagg accatctgga gtaggtaaag gtactgttag aaaacgaata
     2881 tttgaagatc caagtacatc atataagtat tctatttcaa tgacaacacg tcaaatgcgt
     2941 gaaggtgaag ttgatggcgt agattacttt tttaaaacta gggatgcgtt tgaagcttta
     3001 atcaaagatg accaatttat agaatatgct gaatatgtag gcaactatta tggtacacca
     3061 gttcaatatg ttaaagatac aatggacgaa ggtcatgatg tatttttaga aattgaagta
     3121 gaaggtgcaa agcaagttag aaagaaattt ccagatgcgc tatttatttt cttagcacct
     3181 ccaagtttag aacacttgag agagcgatta gtaggtagag gaacagaatc tgatgagaaa
     3241 atacaaagtc gtattaacga agcgcgtaaa gaagttgaaa tgatgaattt atacgattac
     3301 gttgtagtta atgatgaagt agaacttgcg aagaatagaa ttcaatgtat tgtagaagct
     3361 gagcacttaa aaagagagcg cgtagaagct aagtatagaa aaatgatttt ggaggctaaa
     3421 aaataatgtt aaatccacct ttaaaccaat taacgtcaca aattaaatca aagtatttaa
     3481 ttgcaacaac tgcagcgaaa agagcgcgtg aaattgatga acaacctgaa actgaattat
     3541 taagtgaata tcattcattt aaaccagttg gtagagcgtt agaagaaatt gctgacggta
     3601 aaattcgccc tgttatttca agtgattatt atggtaaaga atagtttcga ccattaaaaa
     3661 tatgtgaagc tagacgtaca tcaaatacgt caaggcatat ccaaatcaaa cgcacctatg
     3721 taggtgtgtt tttttagttt atccaatgat tgatgttata ataatactaa atttgtatct
     3781 ataaaaaagt aatgagcatt tgtgcgcata tgatgatgta aagcgtaaat gtcatagtga
     3841 tatttaaggg agattgaata tgaagaaaat attattagcc gttacaggtg gcattgcggc
     3901 atataaagca attgatttga caagtaagtt aacacaatct gggtatgaag ttcgcgttat
     3961 gttaacgaat cacgcacaaa aatttgtgac accattagca tttcaagcaa taagtcgaaa
     4021 tgctgtttat acagatactt ttatagaaga aaatccttca gaaatacagc atattgcatt
     4081 aggtgattgg gcagatgcaa tcattgttgc acctgcaacg gcaaatacaa ttgcaaaatt
     4141 gagtgtaggt attgctgatg atttggtgac atcaacgttg ctagcaacag agacaccgaa
     4201 atttattgcg cctgctatga atgtgcatat gtatgaaaat aaacgtacgc agcaaaatat
     4261 taatatttta aaagaagatg ggtatcattt tatcgaacca ggaagcggat ttctagcatg
     4321 tggttatgtt gctaaaggac gtatggaaga accgcttcaa atcgtttctg taattgatgc
     4381 tcattttcaa aatagtaatc gtttagctaa tagttcattt caagataaac gcgcattggt
     4441 tacagcagga ccaactattg aagttatcga tccagtcaga tttgtatcca atcgttcttc
     4501 tggaaaaatg ggctatgcaa tagctgaagc attgcgaaat cgaggagcta tcgtgacgtt
     4561 agttgctggt cctacaacac tagaggatcc aaaagatatt gaagttattc atgttcaaag
     4621 tgctgaagaa atgtttgaac aagtgacaag ccgatttgac gaacaagata ttgttgtaaa
     4681 agcagcagcc gtatctgact atacaccggt tgatgtatta gaacataaga tgaaaaagca
     4741 ggatggtgat ttgtcagtat cttttaaacg tactaaagac attcttaaat atttaggtga
     4801 acataaaaca tcacagtatt taataggctt tgcagcagag actgaagata ttgaaaatta
     4861 tgcacaacaa aaattacgca agaaaaatgc agatgtgatt atttcaaata atgttgggga
     4921 tatgtctatc ggatttagtt ctgatgataa tgaattgaca atgcatttta aaaataatga
     4981 aaaagtaaat atcaagaaag gaaaaaaagt agtattagct gcacaaattt tagatgaact
     5041 agaaactagg tggcaataat gatagcgaaa gtcatagtcg atgtcgcgtc gaagagcgtt
     5101 gactataaat ttgattatat aattcccgaa caactcgaat ctgtcatcca acctggtgtg
     5161 cgtgtaattg taccttttgg accaagaacg attcaaggtt atgtaatgga agtaacagca
     5221 gaacctgatg cacaacttga cgtttcgaag ttaaaaaaaa tcatagaagt gaaagatata
     5281 caaccagaat taacatcaga attaatagct ttaagtgagt ggatgggttc aactcatgtc
     5341 attaaacgta tttctatgct agaagtgatg cttccgagtg ctattaaagc gaagtataaa
     5401 aaagcattta agatgaaaga tgacatagag ctaccttcag ctttattaca aaaatttgat
     5461 aagcatggtt actattatta taaagatgcg caaaaaaata atgatattca attgcttatg
     5521 aagttgttaa aagatgatat cgttgaagaa aaaacgattc tcacacaaaa tataactaaa
     5581 aaaaccaagc gtgctgttcg tgtcattgaa gggtatcatc ctgatgaagt attagctaag
     5641 ttggagaaag ttattaaaca atacgatttg tatgcttact tgtctgaaga acaacataaa
     5701 acaatatttt taactgatat tgaggatatg ggcttttcaa aatccagttt agatggactt
     5761 atcaaaaaag gttatgttga aaaatatgac gcggttgttg aaagagaccc atttaaagat
     5821 cgtgttttcg aacaagaatc aaaacagcaa ttaacagaag accaatataa agcatatgaa
     5881 gcgattaaag ctaaaattgt aagccaagag caagaaacat ttttacttca tggtgtgacg
     5941 ggatcaggta aaacagaagt atatttacaa acgatagaag atgttttaag ccaaggaaaa
     6001 caggcgatga tgttagttcc tgaaatcgct ctaacaccgc aaatggtttt acgcttcaaa
     6061 cgtcgatttg gtgatgacgt tgctgtatta cattctggct tatctaatgg ggaacgttat
     6121 gatgaatggc aaaaaattag ggatggtcgt gcgagagtaa gtgttggtgc aaggtcaagt
     6181 gtgttcgcac ctttcaaaaa tttagggtta atcatcattg atgaagaaca tgaatctaca
     6241 tataaacaag aagattatcc gagatatcac gctagagaaa ttgcccaatg gcgaagtgaa
     6301 tatcatcact gtccagtcat tttaggaagt gcaacaccat gtcttgaaag ttatgcacga
     6361 gctgaaaaag gcgtttatca tttgctatca ttaccaaaca gagtgaacca acaagcttta
     6421 cctgaaattg atatagtaga catgcgtgaa gaattgagtg aaggtaatcg gtcaatgttt
     6481 tcaaaagatt tacgtgaagc catacaatta agattagatc gacaggaaca agttgtttta
     6541 tttttaaatc gacgtggtta tgcatcgttt atgttatgtc gggattgtgg atatgtaccg
     6601 caatgtccaa actgtgatat ttcattaacg tatcataaaa cgacagactt attaaaatgt
     6661 cactattgtg gttaccaaga gacgccaccg aatcaatgtc caaattgtga gagtgaacac
     6721 attcgacaag taggtactgg tactcagaaa gttgaagaac tattgcaaca agaatttgaa
     6781 gatgcgcgca taattaggat ggatgtagat acaacctcaa agaaaggtgc acatgaaaag
     6841 ttattgactg aattcgaaaa aggtaacggt gacattttac taggtactca gatgattgcg
     6901 aaaggattag attatccaaa tattacttta gttggtgtgc tgaatgcaga tacaatgtta
     6961 aatttacctg attttcgggc gagcgaacgt acttatcaac tattaacgca agtggctggt
     7021 agagctggtc gtcatgaaaa ggcaggtcaa gtcatcattc aaacgtataa tccagatcat
     7081 tattcaatat tggatgttca aaaaaatgat tatttaacat tttatcgtca ggaaatggaa
     7141 tatcgtaaat taggaaagta tccaccgtat tattatttga ttaatttcac aatctcacat
     7201 aaagaaatga agaaggttat ggtagcatcg cagcatgttc ataaaatttt attacagcat
     7261 ttaacagaaa aagcgcttgt actaggtcca tctccggcag cacttgcgag aatcaacaat
     7321 gaatttagat tccaaatttt agtgaaatat aaaagtgaac ctggattatt acaagccatt
     7381 cagtttttag atgactatta ccatgaaaaa tttataaaag aaaaattagc attgaagatt
     7441 gatattgatc cacagatgat gatgtaacat tactaattat tagaaacaag ttcagtatgt
     7501 acgagtattt gaaccagtgt ttgaatattt actttaagta cagaaaaagg gcagaatata
     7561 caactgttaa ctatttaaat tagcagttta tattctgtct ttttatatgg ctttataact
     7621 tacgtgattt tggtttgata aggaatttat tagtattttc atttacgaat tctgatttta
     7681 atgcaagcgc catacttact gatgaataaa agtactaatg attaaacaaa ctatttttaa
     7741 atggcaaatt gaaatagtgc gaattttaaa cttataaatt aatggctat
//
LOCUS       DAKTLH010000123         7786 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.123, whole genome shotgun sequence.
ACCESSION   DAKTLH010000123 DAKTLH010000000
VERSION     DAKTLH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7786
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.123"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(62..706)
                     /locus_tag="O0394_002150"
     CDS             complement(62..706)
                     /locus_tag="O0394_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCY0500218.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            828..1571
                     /locus_tag="O0394_002151"
     CDS             828..1571
                     /locus_tag="O0394_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCY0500219.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            1565..2401
                     /locus_tag="O0394_002152"
     CDS             1565..2401
                     /locus_tag="O0394_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCY0500220.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            2398..3327
                     /locus_tag="O0394_002153"
     CDS             2398..3327
                     /locus_tag="O0394_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCY0500221.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(4487..6529)
                     /locus_tag="O0394_002154"
     CDS             complement(4487..6529)
                     /locus_tag="O0394_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCY0500222.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
     gene            complement(6869..7306)
                     /gene="mnhG2"
                     /locus_tag="O0394_002155"
     CDS             complement(6869..7306)
                     /gene="mnhG2"
                     /locus_tag="O0394_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HCY0500223.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDAHTHASILLSSNEQNSTEALQLRAEKREEHRKKWYQND"
     gene            complement(7281..7583)
                     /gene="mnhF2"
                     /locus_tag="O0394_002156"
     CDS             complement(7281..7583)
                     /gene="mnhF2"
                     /locus_tag="O0394_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HCY0500224.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            complement(7580..>7786)
                     /gene="mnhE2"
                     /locus_tag="O0394_002157"
     CDS             complement(7580..>7786)
                     /gene="mnhE2"
                     /locus_tag="O0394_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HCY0500225.1"
                     /translation="SYETRLTSDWSITFLTILIIITPGSTVIRISQDSKKFFIHSIDV
                     SEKEKDSLLRSIKHYEDLILEVSR"
BASE COUNT         2709 a         1328 c         1149 g         2600 t
ORIGIN      
        1 aaagcaatta tccatatgtc tttatcagtt caatcatgga tatctttatg gcttctttat
       61 tttatttttc agcaaacatg ttcattgcat tttcgtaact gacaataatt acattatcat
      121 tacgtttaat gataattact ttattcattt catctttcga tacaatttcc acttcattac
      181 caatagaaat atctttacta gacaaatata ttagcaaatc ggtcttatct ctcacacgtt
      241 tgattgtaac gatatcacca ggttcataat tcaaaatcgt tgttatatat ttctctttat
      301 attcattatt tctaggaatc acaccgccgt gcgggcaagt ttctgggaaa tttaacaggc
      361 tatccagcct ttcaacaaat aaatctgaaa ttcgatgttc taaaatttct gcttcttgat
      421 gtacttcttc ccaattatat ttcaatattt ctattaaaaa taattctaat agtcgatgtc
      481 tcttaatgat atcaagcgta tgcgttaaac catcctctgt taatctaaca cctttgtatg
      541 gttttgtttc aacatagcct gctttttcaa gacgtcctac catttcactt acagatggag
      601 gcttaatatt taaaaattga gataagattt tatttgtcac aaagttttta tcgccattat
      661 tcgtaaggat tgcctttaaa tagtcctctt tttcttcagt taacatactt tcacctcaca
      721 tacattgtct atattatatc acgaatttac ttgacatgat aaatattctc agtttattat
      781 acaattaatt aggttagcct aaacttttaa ttaggaggta taaacgtttg ttagaaacaa
      841 aagatttaaa tctgttttta ggtaataagc atgtacttaa aaacatttcc ttatcgatac
      901 cagtacgcgg cgaaataatt ggtatcatgg gcccgaatgg tgctggtaaa tcttccctta
      961 tcaagtcttt aattggtgaa tttaatgcta ccggtactaa attgttatat aacaaaccta
     1021 tacaacaaca actgcaacat attacatata ttccacaaaa agcacatatt gatttagatt
     1081 ttcctataag tgtggaacaa gtgattttat caggttgcta caaagaaatt ggatggttta
     1141 gacgacctaa taaatcagca agggataaac tcaaacagtt attaagcgat ttagaattag
     1201 aatctttacg tcatcgacaa atttcagaat taagtggtgg acaattacaa cgtgtgctag
     1261 tagcaagagc attgatgtcc gaaagtgaag tttattttct agatgagccg tttgtcggaa
     1321 ttgattttag tagcgaaaaa ttaatcatga caaaaatcga gaacttaaaa caacaaggaa
     1381 aacttattct tatcatccac catgatctat caaaagcaaa gcaatacttt gatcgcatta
     1441 ttctattaaa tcaaacatta cgatactttg gtgatagtga agaggctatg agtgtcactc
     1501 gcttaaacga aacatttatg agtagcactg actgtagtga ccctagtcaa aggagcaata
     1561 taacatgtta gagtttgtcg aacatttatt tacatatcaa ttcttgaatc gagcattgat
     1621 aacttcaatt attgtaggga tagtttgtgg tacagttggt agtttaattg tattacgtgg
     1681 tctttcatta atgggagatg caatgagtca cgcagtatta cctggtgttg ccctatcatt
     1741 cttatttggt attccaatgt ttgtaggcgc acttataact ggtatgatcg caagtatttt
     1801 tatcggttat atcacatcta gtagtaaaac gaaacctgac gccgctattg gaattagttt
     1861 taccgctttc ttagcttcgg ggattattat tattagctta ataaacacta cgacagattt
     1921 gtaccatatt ctgtttggta atttactggc aatcacaaat agtgcatttt taacaactat
     1981 tgtgattggt tcaatcgtcc ttattctaat cattattttc tatcgtccat taatgatttc
     2041 tacattcgat ccaacgttta gtagaatgag tggtctaaat acgacgttat tacattactt
     2101 tgtgatgttg ttactctcat tagtaacagt agcaagtatt caaacggtag gtattattct
     2161 tgtagttgca ttattaatta cacctgcatc tacagcattt ttaattagta aaaaactata
     2221 ctcaatgatg attatagcaa gtttaataag tgtcataagt tcaattgtcg gactttatta
     2281 cagctatatt tacaacattc ctagtggtgc gacaatcgta ctttgtacgt ttgtaattta
     2341 tatcatcaca ttatttttca caaaatttac gaatagaaag aaacgaggaa gtttaacatg
     2401 aaaaaattag tacctttatt attagcctta ttacttctag ttgctgcatg tggtactggt
     2461 ggtaaacaaa gcagtgataa gtcaaatggc aaattaaaag tagtaacgac gaattcaatt
     2521 ttatatgata tggctaaaaa tgttggtgga gacaacgtcg atattcatag tattgtacct
     2581 gttggtcaag atcctcatga atatgaagtt aaacctaaag atattaaaaa gttaactgac
     2641 gctgacgtta ttttatacaa cggattaaat ttagagactg gtaacggttg gtttgaaaaa
     2701 gccttagaac aggctggtaa atcattaaaa gataaaaaag ttatcgcagt atcaaaagat
     2761 gttaaaccta tctatttaaa cggtgaagaa ggcaacaaag ataaacaaga tccacacgca
     2821 tggttaagtt tagataacgg tattaaatac gtaaaaacaa ttcaacaaac atttatcgat
     2881 aacgacaaaa aacataaagc agattatgaa aagcaaggta acaaatacat tgctcaattg
     2941 gaaaaattaa ataatgacag taaagacaaa tttaatgaca ttccaaaaga acaacgtgcc
     3001 atgattacaa gtgaaggtgc cttcaagtac ttctcaaaac aatacggtat tacaccaggt
     3061 tatatttggg aaattaacac tgaaaaacaa ggtacacctg aacaaatgag acaagctatt
     3121 gagtttgtta aaaagcacaa attaaaacac ttattagtag aaacaagtgt tgataagaaa
     3181 gcaatggaaa gtttatctga agaaacgaag aaagatatct ttggtgaagt gtacacagat
     3241 tcaatcggta aagaaggcac taaaggtgac tcttactaca aaatgatgaa atcaaatatt
     3301 gaaactgtac acggaagcat gaaataacac gctgtgtttt aatgaagtaa gatgaattga
     3361 tgttgatgca acctaaaata ttggtatctc caatatttta ggccacacat caacataaca
     3421 aagtcgaagg ctaatagtcc catatcgtgc gttaaatata tattaccctc ctattaatat
     3481 atataccgtt cccgatcgca cgatatggtg gtattagaac ttctctttga acgaaagaga
     3541 aaagctagaa gttcttatgc agttttaatt aaactgtaaa catttgtcac tctttaaatc
     3601 aaagagtaaa gttaaaagct ttatgtggtt ttgattaaac tgcgaacagc tgcttctctt
     3661 tgaacgaaag agaaaagcta gaagttctta tgcagtttta attaaactgt aaacatttat
     3721 cactctttaa atcaaagagt aaagttaaaa gctttatgtg gttttgatta aactgcgaac
     3781 agctgcttct ctttgaacga gagagaaaag ctagaagttc ttatgcagtt ttaattaaac
     3841 tgtcgttccc ttcatctctt ttaaccacag agatgcgtta gaagttcttc taatacaatt
     3901 tatacaacgc cattccctac acactcttat aaaagagatt cacgcgcgtc aataaattgt
     3961 attacatact aactaaaaag cttttcttaa tcgtactaac gaagttagag gttcttatgt
     4021 gattggacaa ctgttctccc tactagactt ttagcatagc tggttaaaag tactattaca
     4081 gattgttcaa ttgtatgccc ctttcctttg aacggaatat taaaagctat gtgcagtaac
     4141 ttttaatagg cataccactt ttgcatcaag ctataaataa tcgcatactt tgatatttaa
     4201 tctggcctat caaagtaaac atctatggtt tcctcccccc atagatcctt aagccacact
     4261 catccgatgt catatgagtg tggctatttt ttgtgaatac acttaacttc acctttaaat
     4321 gacttcgctt aatctatctc ctccaatttt taaaacttat ttcaattaaa tctcaaatat
     4381 taataattcg tatagtttaa aactatctat tcaattcccc taaaataaaa gggattgatg
     4441 aaaagccctt cagtttttca tcaatccctt ttagcataat taggaactac gtcatactat
     4501 ctacaataac catttcatca taattgatgg cttctcttag ttttaacgct gtaccttctg
     4561 atacttcatc tgtatcaatc aactcacgta aaatgcgacg ttgttctctc aatgcattta
     4621 atttaatttt agtaatcgta tcttcacttg ctgagttgaa gaaatttgat ggtgttaagt
     4681 tttcaattcg cattaaataa ccgtcacaaa ccattccaac ttcaagtgta ttatcttttg
     4741 ttgtttcctt agacaaacga ctaaccacat tataatggac taatttatta attttattta
     4801 attccattaa gttatccgta acactaagtg aagatgcgga gttcactctc gtttgtactc
     4861 ttcgttttaa taaagcacct cgtaataata caatcattct tcttattaat gatgcttgtc
     4921 tatatacctg tgttctttca gcataacgca tatagttttc aagtacacta ttcgttattt
     4981 gtccttcatc tactaaagac tctaatgttt ttgtttctac attaaaagca attttttgta
     5041 gacgttctaa ttctttagag ttttcatcat ctttctctac agtttttaaa aatgctaatt
     5101 tatcatgata ttctttaatc acgttaccat atttaaaact tgtttcgaaa gtagattttt
     5161 gatttagata atcaataact tgttctaata tataaattct agcaacttta aacgacatat
     5221 tgccaattac tgttttaggt gcaggtttcg ttaataatgg caataatact tgcgcaacta
     5281 ccaaactaat aataaccata ccagatgcaa taaataataa gtcgtttcta tacgtaaaag
     5341 catgatgccc tgctaaaaaa tacggtaacg ttaatgcgat tgctaaagaa attgttccat
     5401 gcacaccaca taacgtcata attaaagcgt ataaacttcg ctttggtggt ttctcagtcg
     5461 ttggattatc atcatcattt ttagtcatca ttttttggaa tggactgatg gctaaataaa
     5521 aataaggata taagacataa acccaaacaa atctaaatag atagacagct aaagcaacaa
     5581 caatagtgat gcctattaaa aagattaaat tgtgcggttc tgttttgata attttaataa
     5641 taacttcagg tactaaaaat cctaatattg aaaaaacaaa gccatttaaa acataaccta
     5701 gtatattcca tgtatgattg taactcattt gcagttgtgt acgtacttgc ataattctgt
     5761 cacgttcgaa accatgtaca agtcctgcaa ctactgctgc aatgattcct gatgcgtgaa
     5821 acaattcagc aattaaatac gtaacaaatg gtgttaacaa ttgaataatt gtaaacatat
     5881 taatgttttc atatcctcga cgcatcaatg ttaatcggaa ccttactaat gccataccta
     5941 taagtaaacc aaccactgcg ccaccaattg atgcaattaa aaacaactga acagcatcaa
     6001 caagtgaaaa agcacctgta actaatactc caacagctat tttaaatgaa ataataccag
     6061 cagcatcatt caataatgac tcaccttcaa gaattgtcat tgctcctttt ggcaagacct
     6121 ttcctttagt gattgcttgc actgctactg catcagtagg acaaagaatg gcagcaattg
     6181 caaatgctgc tccaataggt aaatctggcc aaatccaatg aataaataaa cctacaccta
     6241 tcacagtagt aatgactaat cctaatgcca tcatcatcac tggcttaata tatttcctta
     6301 aatggactct agaaacatta acaccttcta caaataacaa aggcgcaatc attgttacca
     6361 taaacaattc agaatcaaaa ttaaattgaa cagggattgg ggtaataaat agtaacatgc
     6421 ccaagaaaat ttgtataaat gctaggggta ctttaggtat gaaagtatgg acaaacgaac
     6481 ttagtatcac aacagctata aatataagaa ttgtttcaaa tatttccaaa ctttcacctc
     6541 tctaaaaagt attgtttaat tgaaaattaa gtatcacatc tcgttgtaat tatactttag
     6601 aggataaatt gagttagcga ccacaaaagc actttaatat agatatatgt ctacgattgc
     6661 agtacttaaa tttgcaatta tttaatttta ttttatcact aattgtttgt ataaataaac
     6721 aacttgcttt cacataacaa cattaactta taatacaaaa aatgagcacc ttaaaatcga
     6781 ctaaccaatt tcaaagtact cttttaatga ttaattttga aaacagattt tcaaaagcat
     6841 tgttatgctt aacaatttag ccaacacttc aatcgttttg ataccatttc ttacgatgct
     6901 cttctcgttt ttcagcacgt aattgtaatg cttctgtaga gttttgttca tttgaactta
     6961 ataatattga tgcatgtgtg tgagcatcat tttttcgata catataagcg ccgttgcgat
     7021 aagcagcgcg agcgactaag tgcatgccga ctggtgaagt taaattaata aaaacaagtg
     7081 acagtaataa acgcacactg aaaaatcctg tattcacaat aaaataaatt aatacaccga
     7141 ttaaagttaa taacacggat aaagttgaac tttttgtcgc agcgtgactt cttaagaaaa
     7201 catcttggaa tttcacgata cctattgcac taataagagc aataaaacta cctaacaaca
     7261 acatcacagc agcaataaga ctaaagattt cttttgttat ttccattaaa cacatgcccc
     7321 ccaccaataa agcgtgatat tgaaacagaa cttacaaaag atataatggc aatgagcatg
     7381 attgaatcta agaaagaaac ggtgcccata agtacactta acacacccac aattgacatt
     7441 acgacagcac ttgttgtatc aaatgtaacg acacgatctg ctgttgtagg tcccttgatt
     7501 aatctaaata aacagatgat taatgcaatt ccaaaaataa tgagtgaact aataatcata
     7561 atatgtgtta ttgtttgtat catcgcgaca cctccaatat taagtcttca taatgcttaa
     7621 tacttcttaa caaactatct ttttcttttt ctgacacgtc gatactatga ataaaaaact
     7681 ttttagagtc ttgagaaatt cgtattactg tagaccctgg agttataata attaaaattg
     7741 ttaaaaatgt tattgaccaa tcacttgtta gtcttgtttc atatga
//
LOCUS       DAKTLH010000124         7623 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.124, whole genome shotgun sequence.
ACCESSION   DAKTLH010000124 DAKTLH010000000
VERSION     DAKTLH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.124"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            149..418
                     /gene="rpsN"
                     /locus_tag="O0394_002158"
     CDS             149..418
                     /gene="rpsN"
                     /locus_tag="O0394_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCY0500226.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            575..1552
                     /gene="guaC"
                     /locus_tag="O0394_002159"
     CDS             575..1552
                     /gene="guaC"
                     /locus_tag="O0394_002159"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCY0500227.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKTHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            1574..2590
                     /locus_tag="O0394_002160"
     CDS             1574..2590
                     /locus_tag="O0394_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCY0500228.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMISYMKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMANNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTG"
     gene            complement(2970..3593)
                     /gene="lexA"
                     /locus_tag="O0394_002161"
     CDS             complement(2970..3593)
                     /gene="lexA"
                     /locus_tag="O0394_002161"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCY0500229.1"
                     /translation="MRELTKRQSEIYNYIKQVVQTKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            3736..3969
                     /locus_tag="O0394_002162"
     CDS             3736..3969
                     /locus_tag="O0394_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500230.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANISAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
     gene            4105..4344
                     /locus_tag="O0394_002163"
     CDS             4105..4344
                     /locus_tag="O0394_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCY0500231.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            4465..6453
                     /gene="tkt"
                     /locus_tag="O0394_002164"
     CDS             4465..6453
                     /gene="tkt"
                     /locus_tag="O0394_002164"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCY0500232.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            6731..6973
                     /locus_tag="O0394_002165"
     CDS             6731..6973
                     /locus_tag="O0394_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCY0500233.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            6960..7058
                     /locus_tag="O0394_002166"
     CDS             6960..7058
                     /locus_tag="O0394_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500234.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYVY"
     gene            7152..7619
                     /locus_tag="O0394_002167"
     CDS             7152..7619
                     /locus_tag="O0394_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HCY0500235.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
BASE COUNT         2800 a         1033 c         1369 g         2421 t
ORIGIN      
        1 atgttcgata atacacttta atcttaaata tgatggttta gaaaatgatt taacaaagaa
       61 atgaaacttt actgttgaat tatgtgagga ttgtgttatt atataaatcg taataattac
      121 gatttgataa aaagtgaggt aactatatat ggctaagaaa tctaaaatag caaaagagag
      181 aaaaagagaa gagttagtaa ataaatatta cgaattacgt aaagagttaa aagcaaaagg
      241 tgattacgaa gcgttaagaa aattaccaag agattcatca cctacacgtt taactagaag
      301 atgtaaagta actggaagac ctagaggtgt attacgtaaa tttgaaatgt ctcgtattgc
      361 gtttagagaa catgcgcaca aaggacaaat tccaggtgtt aaaaaatcaa gttggtaaaa
      421 tcaaatttcg tactttagcc catttacaat atcaataaaa caatgtacag tatatacgaa
      481 tgctataaac tgaatgtttt ctcatattaa taaggaaaca ttcggttttt aatttgcatt
      541 taaaaataat tagtttttaa aggggctatt taaagtgaaa atatttgatt acgaagatat
      601 tcaattaata cctaataaat gcatagttga aagtaggtct gaatgtgata caactatcca
      661 atttggtccg aaaaaattca agctacctgt agttcctgca aatatgcaaa cagttatgaa
      721 tgagaaatta gcgaaatggt ttgctgaaaa tgattacttt tatatcatgc atcgttttga
      781 tgaagaagca agaatacctt ttataaaaca tatgcaaaat tcaggcttat ttgcatctat
      841 ttcagttggt gtaaagaaag cggaatttga ttttattgaa aagttagctc aagaaaaatt
      901 aatccccgaa tatattacaa tagatattgc gcatggtcac tcagattcag tgataaacat
      961 gattaaacat ataaaaaccc atatacctga tagttttgtt attgctggta atgttggtac
     1021 gccagaaggt gttagagaat tagaaaatgc tggtgctgat gctaccaaag tcggtatagg
     1081 tcctggtaga gtttgtatta caaagattaa aacaggtttt ggtactggtg gttggcagtt
     1141 agcggcatta aacatatgta gtaaagcagc tcgtaaacct ttgattgccg atggtggtat
     1201 aagaacgcat ggcgacattg ctaaatcaat tagatttggt gcatcaatgg tcatgattgg
     1261 ttcattattt gcggcacacg aagaatcacc tggtgaaact gtagaacttg atggtaaaca
     1321 gtataaagaa tattttggta gtgcatctga atttcaaaaa ggcgaacata aaaatgtaga
     1381 aggtaaaaaa atgtttgtag aacataaggg ttcattaatg gataccttaa aagaaatgca
     1441 acaagattta caaagctcaa tttcatatgc cggtggaaaa gacttgaaat cattacgtac
     1501 tgtagattat gttattgtta gaaactctat tttcaacggt gatagagatt aatatttata
     1561 gtaggtgatg taaattaaaa aattcatagt aactgttgtt gcgtttttat caattattat
     1621 cattgcgcca ataacagaat ttaaaccatt cattcattta caaaatgaag taagacaata
     1681 tattgacatt cacatcaata aagaaacaat ttctgcggaa aataaattgg atacaccgaa
     1741 gaaacaacaa tttgccttta ataatataca aatgaacatg tcgaaatcag atgttgagaa
     1801 aacattaaat aaaccaaaaa gagtgacatt taatgaatat ggtacgaagt ggtatacgta
     1861 ttatgatgac gattacaata attttataat gataagttac atgaaagata aagttaatgc
     1921 gttatataca aatcaaaata taatcacttc aaaatcaaaa attaaatata atacacctaa
     1981 atcggttgta aggcaaagat taggcgaacc agaaacagag attgttaaag gtagagtgcg
     2041 ttacgaacaa aataataaag aatatgatgt tttccataaa aatcacattt atacgacggt
     2101 attttatgat aagcatcgac gtaataatgt aacagctgtt ttacaagtaa gtgatgctat
     2161 ggaaaataga ttaaaagaac aatatggagc accatcgaaa tcgcttgcag atagttttga
     2221 actacaaaat tttgatttag ttaatgctga aagaaaacaa catcaattat ctacattgaa
     2281 gtattctaaa cagaattctg aaactgcacg taagcatagt aaagatatgg ccaacaatca
     2341 ttattttgat catacaaatt taaaaggtca atcaccattt gatcgattga aaaaagatgg
     2401 tattacattt aactcagccg gagagaattt agcatatggt caagttagta gtatctatgc
     2461 acatcaagga ttaatgaatt ctattggtca cagaaaaaat attttaaatg atacgtttaa
     2521 aatattaggt gttggtgttg attttaatga tgaaaaacaa cctttttgga cagaaaatta
     2581 tactggttaa tgtttttggc atgagattaa agggtaatgt ttgtctgtaa ggaagtagat
     2641 aaacatgatg acaacagata aaccaaaaga tgcggatata cttgaacgcg tcaaagatat
     2701 acttaataaa aaagaaagaa aaaaataata atggctcgct cctgtaaatt attacggggg
     2761 cgagttttaa tttgtgtaac attttgcaga aatctgtaat tcaaaattta tattaaagaa
     2821 tgagattatc taacaattcg caatttctgt ttgaactgaa tttagattag aataaataaa
     2881 acctcttgaa aacgtaactt tttcaagagg tccaaaattt ataaaaatga gggcttaaca
     2941 attacgataa tatataacgg tttaaaatat tacatttcgc ggtacaaacc aattactttc
     3001 ccaattacag caacattgtc taggtaaatt ggctccattg tactattttc aggttgtaat
     3061 cgataacgat ttttttcttt atagaagcgt ttgacagttg cttcatcttc ctcagtcata
     3121 gcaacaataa tgtctccatt ttctgctatg gtttgactgc gaacaattac tttgtctccg
     3181 tctaatatac cagcctcaat catactgtcg cctacgacgt ttaatatgaa tatgtcgcta
     3241 ttgtgtgtcg atgttaagtg ttcaggtaat ggaaaatatt cttcaatatt ttctactgcg
     3301 gtaataggaa cacctgctgt gactttacca ataactggca catgaatcgt ttcttccata
     3361 ttaatattat catttgtttg atcacttaca atttctatag cacgtggttt cgttggatct
     3421 cttcttatat agcctttttc ttcaagacgt gaaaggtgac catgaacagt tgaactggat
     3481 gctaagccaa ctgcttcacc aatttcgcga acactaggcg gataaccttt cgtttgaaca
     3541 acttgtttaa tatagttata tatttcgctt tgtcgttttg ttaattctct catatatagg
     3601 cactccctaa attaatttaa atacagtata tcattaattt gaaaccaaaa caaacatttg
     3661 ttcgttaaaa tattgacaca gaacataagt tctgataaat tactaataca aacaaatgtt
     3721 ctaggagtga aaatgatgtt ttacaataaa tataaaaacg tatcaacata tatcatcata
     3781 tttttagttt caagtgcagc ctttgcaata ttcttgttaa gtgcgaacat tagtgctcac
     3841 tcggaacaag tgtacgaaat gactgaccat caaattaaga acaatacgat aaataaagca
     3901 tacgaacata aagaccctac aaacaatagc gaacaaagag atgggaaagt gttcgcttta
     3961 ataaattgat acattgtcac aacgttattt tgcctatttt tgcgaaatag cgttttttat
     4021 tacttttttg ctgatcttaa atttgttata ttttgttaaa gtattataat gattgaataa
     4081 acaaattgaa ggtaggtttt ttaattgagt aattctgatt tgaatatcga aagaattaac
     4141 gagttagcta aaaagaaaaa agaagtagga ttaactcaag aagaagcaaa ggagcaaaca
     4201 gccttaagaa aagcttatct tgagagtttt agaaaagggt ttaaacaaca aattgaaaat
     4261 actaaagtaa ttgatccaga aggtaatgat gtaacacctg aaaaaattaa agagatacaa
     4321 caaaaaagag ataataaaaa ttaaatcaca aatctgtaaa gaattttctg acattataac
     4381 ttgaaataag tattttactt atctttttat tttaaaataa gttataatgt atttgataaa
     4441 attgaagaag ggaagataca caagatgttt aatgaaaaag atcaattagc tgttgatacg
     4501 ctacgtgcac taagtatcga cacaatcgaa aaagcgaatt ctggtcatcc aggattacct
     4561 atgggagctg ccccaatggc ttacactttg tggacacgtc atctgaattt taatccacaa
     4621 tctaaagatt acttcaatag agaccgtttc gtattatctg cagggcatgg ttcagcatta
     4681 ttgtatagct tgttacatgt ttctggtagt ttagaattag aagaattaaa gcaatttaga
     4741 caatggggtt ctaaaacacc aggtcatcct gaatacagac atacagatgg tgtagaagtt
     4801 actaccggac cacttggaca aggttttgct atgtcagtag gattagcttt agcagaagat
     4861 cacctagcag ggaaatttaa taaagaagga tataatgttg tagatcatta cacatatgta
     4921 ttagcttctg acggtgattt aatggaaggt atatcgcatg aagcagcttc atttgctgga
     4981 cataataaat taagtaaatt agttgtttta tacgattcaa atgatatttc attagatggc
     5041 gaattaaaca aagctttttc tgaaaacaca aaagctcgtt ttgaagcata tggttggaat
     5101 tacttactag ttaaagatgg taatgattta gaagaaattg ataaagcgat tactacagct
     5161 aaatctcaag aaggaccaac gattattgaa gttaaaacaa caatcggatt tggttcaccg
     5221 aataaagcag gaactaatgg tgttcatggg gcacctttag gtgaagttga aagaaaatta
     5281 acattcgaaa attacggttt agatcctgaa aaacgtttta atgtttcaga agaggtatac
     5341 gaaattttcc aaaatactat gttaaaacgt gctaatgaag atgaatctca atggaattca
     5401 ttattagaaa aatatgcaga aacatatcct gaattagcag aagaatttaa attagcgatt
     5461 agtggtaaat tgcctaaaaa ttataaggat gaattaccac gttttgaact gggtcataat
     5521 ggtgcatctc gtgctgattc tggtactgtt attcaagcaa tcagtaaaac tgtcccttca
     5581 ttctttggtg gatcagcaga ccttgctggt tcaaacaaat ccaatgtaaa tgatgcaact
     5641 gattatagtt ctgaaacacc tgaaggtaaa aatgtgtggt ttggtgtacg tgaatttgct
     5701 atgggtgctg ctgtaaatgg tatggctgca catggaggtt tacatccata tggtgcaaca
     5761 ttcttcgtat ttagtgatta tttaaaacca gcgttacgtt tatcatcaat tatgggatta
     5821 aatgcaacgt tcatcttcac acatgattca attgcagtag gtgaagatgg tcctactcat
     5881 gaaccaattg agcaattagc tggattaaga gccattccaa atatgaatgt tatccgtcct
     5941 gctgatggta atgaaacaag agtagcatgg gaagttgcct tagaatctga atctacacct
     6001 acttcattag tattgacacg tcaaaactta ccggtattag atgtaccaga agatgtagtt
     6061 gaagaaggcg ttcgaaaagg tgcctataca gtttatggct ctgaagagac accagaattc
     6121 ctattattag cttcaggttc agaagttagt cttgcagttg aagctgctaa agatcttgaa
     6181 aaacaaggta aatcagtacg tgttgtttca atgcctaact ggaatgcatt tgaacaacaa
     6241 tctgaagaat ataaagaatc agttattcca tcaagcgtaa caaaacgtgt tgcgattgaa
     6301 atggcttcac cgcttggatg gcataaatat gtaggtactg caggtaaagt tattgctatt
     6361 gacggctttg gcgcaagtgc acctggcgat ttagtagttg aaaaatatgg atttacaaaa
     6421 gaaaatatct taaaccaagt tatgagctta taagaataat ttataaagcg agtatgttta
     6481 gaagtctagg atgcataatc ttaggcttct tttaaagtgt tgaaatttag agtatagcac
     6541 ttaaactaca tcataagtga taagttatga aagtatacta tttcagatta atctttaaaa
     6601 gctctgttat aacagcatga tttttgatat tatttttagt atcgatatta aaatacttga
     6661 ataaactagt tcttgaaata atgtgatgaa tttagtaaaa ttcagtagga taaagaaagc
     6721 gggtgaaaca atggcaactt ggttagcaat tatttttata gtagctgcat taattttagg
     6781 tttaattgga ggtttccttt tagctagaaa atatatgatg gactacttga agaaaaaccc
     6841 accaatcaac gaagaaatgc ttcgtatgat gatgatgcaa atgggtcaaa aaccttctca
     6901 gaagaaaatt aatcaaatga tgacgatgat gaataaaaat atggatcaaa atatgaagag
     6961 tgcgaaaaag taaattcgca attgatagag gctattttcc agatatggaa atggcctctt
     7021 tttataatca aattaataag aataaatatg tttattaaaa ttaaagttaa caaaatgacg
     7081 aatagactga gaaatgctat aattcatttt gtatgattta cagagagttt atttaacgag
     7141 aaggtgtctg cgtgctctat ttaatatttt caatcattgt agctttattt atgggaacta
     7201 tagttatagt tattcgtatg aaagctcaaa attatccggt aaatgagaaa aaaatagttt
     7261 tgccaccgtt ttttatggcg accggtgcat tgatgtacgt cgttccatat tttaggctaa
     7321 caggatcgga aatgctagaa gcctttataa ttggtttgct tttttctaca gttctaattt
     7381 ggacttctcg atttgaagtc aaaggtacag aaatttatat gaaacgatct aaagcatttc
     7441 cagttatttt gatttcatta cttatcattc gtactgtgat gaaaatattc attagtaatg
     7501 aaatagatcc tggagaatta ggcggcatgt tctttttatt agcattctgt atgattgttc
     7561 cttggagagc agcaatgcta tataaataca aaaaactaaa gaaaacatta atcaattaat
     7621 tac
//
LOCUS       DAKTLH010000125         7495 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.125, whole genome shotgun sequence.
ACCESSION   DAKTLH010000125 DAKTLH010000000
VERSION     DAKTLH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.125"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(300..1160)
                     /locus_tag="O0394_002168"
     CDS             complement(300..1160)
                     /locus_tag="O0394_002168"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCY0500236.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            1378..1899
                     /locus_tag="O0394_002169"
     CDS             1378..1899
                     /locus_tag="O0394_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HCY0500237.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            complement(2008..3618)
                     /locus_tag="O0394_002170"
     CDS             complement(2008..3618)
                     /locus_tag="O0394_002170"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCY0500238.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCAINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(3954..4484)
                     /locus_tag="O0394_002171"
     CDS             complement(3954..4484)
                     /locus_tag="O0394_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCY0500239.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            complement(4596..5456)
                     /locus_tag="O0394_002172"
     CDS             complement(4596..5456)
                     /locus_tag="O0394_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0500240.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
     gene            5880..6683
                     /gene="coaW"
                     /locus_tag="O0394_002173"
     CDS             5880..6683
                     /gene="coaW"
                     /locus_tag="O0394_002173"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HCY0500241.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLEK"
     gene            complement(6813..7484)
                     /locus_tag="O0394_002174"
     CDS             complement(6813..7484)
                     /locus_tag="O0394_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HCY0500242.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
BASE COUNT         2376 a         1390 c         1044 g         2685 t
ORIGIN      
        1 ttccccactt gaccatggtc taaagtctga ttattcaact aaatattgac gttgttttga
       61 caattattta gttttaattc tacggttttc acgtaaactt ttttcataaa ttgctcattt
      121 tccttataat tatatcacaa tcaattttga tatattttac catttttacg ctttcatttt
      181 aaaatcaaac tgttttattt attacttata cgacataaaa aagaagcaaa caccattttg
      241 tctgcttctt ttaaaattaa tatccctacg ttattaataa cttaaagact aaatattaat
      301 tatttagcgc ggttagaagt accgaactct ttaattttac ctttaactgt ttctttgatg
      361 gcttcacgtg caggtcctaa gtatttacga ggatcgtaaa cttctttgtc gttatttaaa
      421 acgtcacgaa ctgcttttgc tgaagcgatt tggttttcag tgtttacgtt aattttagct
      481 gtaccaaatg gaattgcttt ttggatatct ttagtcggga taccagtacc accgtgtaat
      541 actaatggta aacctgtaga taaaccgatt tcttccattt ctttaaatcc taattttggt
      601 tcacctttgt atggaccatg aactgaacct aatgctggcg ctaatgcatc aataccagtt
      661 ttttcaacta gttcttgaca ttctttagga tcagcataaa tgatgccgtc tgctacaaca
      721 tcatcttctt gtccaccaac agtacctaat tcagcttcta cagaaacacc tttttcatga
      781 gcgtattcaa caactttttt agttgttgct acgttttctt cgaatgggct gtgtgaagca
      841 tcgatcatta ctgatgtgaa accagcatcg atagcttctt tacatttttc aaagcttgaa
      901 ccatggtcta aatggattgc tacaggaata gtgatgttta agtcatgcat taacccttca
      961 accattttaa caattgtgta gaaaccgctc atgtaacgag cagcaccttc agaaacacct
     1021 aaaattacag gtgcattttc ttcttgtgac gcttctaaaa ttgcttgagt gaattctagg
     1081 ttattaatat tgtattgacc taccgcataa ccattttctt ttgcatcaat taacatttct
     1141 ttcattgaaa ctaaaggcat gaaagttcct ccttgagtgc ttacgcacgt atttttcaaa
     1201 gtaattatat caaaaaaata gcgtcaatgc attgaaatac tgcagattaa atgtgtattt
     1261 tttgaaaaat gcgatagtta tactaaatgc aaacgtatac atttttatac aatttaagcc
     1321 gttgcataat atgtagtcat taattacaat attttataag tagattggag gataaagatg
     1381 tcaaaaatct taaacacaca actaataggt atctttaata ggctagaaaa acaatcttta
     1441 gaaattcaaa tggccgccca atgtttaatt caagcaattg gtggcgaagg ttatgtttat
     1501 gtaaaaggtt atgatgattt acagtttttc gaaagtttca ttctacatag tgatgagcgt
     1561 ttaaaatcta gccgaaaact tgacgctatt aaagacttta aagaaattga ctcaactgat
     1621 cgtgtattat tattcgcacc attttataat gatcaagttg cattagatat tcaaaaactt
     1681 atcgacttag atatcgatgt ggtcttaata agcaacaaac ctaaaacaga tgatttccct
     1741 gatcatctcg tacattttat cgatttatct acaccaagac ctatagtcta cactgaagat
     1801 tacgataaga ttgtccaacc acatgcgatg gcattgaatt atgtatacta tgacatttat
     1861 acacaaatga ttgaaatgac aagagactta gaattataaa agtcactatt gttatttcat
     1921 accttgtcac taacataata ttaaaattat acgaaaaacc ggtactttca atgtttaaca
     1981 atgaaagtac cggttttatt agcaaattta tttattttgt tgatatttta atgaagcttc
     2041 aataaatgat ttaaaaatcg ggtgcggacg atttggtcta gataagaatt ctgggtggaa
     2101 ttgacaagca ataaagaaat catttgtcgg aatctctacc atttctacta aacgtccatc
     2161 tggacttgta ccagaaatca ccataccatt tgcttctaat tgttctctat agtcattatt
     2221 aaattcataa cgatgacgat gtctttcttc aatttccgct ttaccataaa catcttgtgc
     2281 caatgtgcct tctttaattg aacatggata taagcctaag cgtaatgtac cacctaaatc
     2341 ttcgatatct ttttgttctg gtaataaatc tataattggg tatggtgttg ctgggtctaa
     2401 ttcagctgaa tgtgcgcctt caaggcctaa tacgtttctt gaaaattcaa ctgttgcaag
     2461 ttgcattcct aaacaaatac caaagaatgg tacattgttt tctctagcat acttaattgc
     2521 actaatttta ccttcacttg cacggaaacc aaatccacct ggtactaaaa taccgtcgac
     2581 atctgcaagg tattcggctg cattttcatc tgttacttca cttgaatcaa tccatctaat
     2641 gtcaatatct ttggcaaaag gatatccagc atgtttcaat gattcaacaa ctgataaata
     2701 tgcatcttgt aagctaacat atttacctac taaaccaatt gtaattttac catctaaatt
     2761 attaacgata tctaacaact gtttccattc atcaagctgt gtttcatatt tcgcgtttaa
     2821 ttgtaaacgt ttaataacga tatcatccat attttgttgg cttaattgta atggaatttc
     2881 gtataaagag tctgcatcac gacattcaat aacactttct ttattaatgg cacagaataa
     2941 tgcaatttta tcttttaaat cttgtgtcat ttcatattca gttcttacaa cgattaagtc
     3001 tggttgaata cctaagcctc gtaattcttt aacactatgt tgtgttggct tcgttttcat
     3061 ttctccagca gctttaatat aaggcagtaa tgtacagtga acatacataa cattttctct
     3121 acctaaatcg ctacgaattt gacgaatcgc ttcaataaac ggtaatgact caatatcacc
     3181 tgttgtaccg ccaatttcag tgataacaac gtctgcattc gtactttccc ctgcaagtaa
     3241 taaacgttct ttaatttcat ttgtaatatg cggaataact tgaactgttc cgcctaagta
     3301 atcaccacga cgttcttttt tcaatacgtg tgaatacact ttaccggctg tcacatttga
     3361 aaacttgttt aaattaatat caataaatct ttcgtaatgt cctaagtcta ggtcagtttc
     3421 tgcaccatca tccgttacga atacttcacc atgttgataa ggactcattg tacctgggtc
     3481 aacatttaag tatggatcga atttttgaat tgttacattt agacctctat cttttaataa
     3541 tctacctaga gaagatgctg taataccctt ccctaatgat gaaactacgc cacctgttac
     3601 aaaaataaat tttgtcatgt cttgacctcc taattattaa agggaatgct tcctcatcaa
     3661 ttgtaaaaat tacgtaaaac ataacattaa agcaagctca ttcaagttgt ttatttgtcg
     3721 aattcgacaa aattaaattc tgtttactta ccgtttccat actgctatgt cactatgtat
     3781 atcatttaca actttgattt ttgcgttatc atttcttgat ttactcaatt aaataaaaaa
     3841 acgctccctc tcacaaattc tgtaagggga gcgtataaat tttatacacg tgtcctattt
     3901 aaaggagccc gaataaaatt ttatcatctt tcaactaaaa gtcaaacaaa aaattaatcg
     3961 ttgaagtctt cttcgtcttc aaacacttct tcgtcttcgt ctagttcatc ttcatcttct
     4021 tcaatgacta tatccgaatg attgatttct tcttcaacct gctcatcttc tggatcattt
     4081 agttcttctt gatcatctgt ttgagctgga atatcatcgt cgtcatccat ttcatcttcg
     4141 cccaataatt ttaagttttg atcttcttca tcatctgcat ccagaatatc gaatttttga
     4201 atagttggtg cgattttctc ttcaatatca tctaccgaat accaatcacg taatccccat
     4261 aaattttctc caacatttaa aaaacgacca tctgtgttta aatccgtgta aaattgtaca
     4321 acacgatttt caatttcttc gtactcataa tcacctaacg ctctaaattc atcgatgata
     4381 tcatataagt tcattgtttc gcctttatca ttcaataatg tataagccat atcaataaat
     4441 gatttttcat caaccatttg ttttgtataa tcttgaattt tcattgccca atacttcctt
     4501 tcgaaggata taatctaaat ttatacgtct gaattcattt atatatagta acaaaactaa
     4561 attataaatg acaatatcat atttcgcaat cactgttagt aatttaaata tttttcgaaa
     4621 acgacataat catcagtttg ttcgatttca acgaaattac tatttaaaca taatggttta
     4681 aaatcattat tctctccaaa cagaatcatc gtaatcacat taaggttgtc atagtttttt
     4741 cttaagtaag ttggcaattg tcgcagagca cttgaaccaa taccattaaa gcgatatgat
     4801 ttatccactt caatagcagc aatgttcatg cgttcaccaa tctgctccaa tgcgataaag
     4861 ccaacctttt ggccatcaat cattaaatta atgatatgta cattggtcac ttctgaacga
     4921 ctttcttctt caagctttaa tgatgaaaca tgttttgaat ctaagtccaa gtaatatagt
     4981 cgttctttgc caattggtcc ttcttcttta gtcgctgtat gcatgaatcc agcacgttcg
     5041 tataggttcc aagcattgtc attttccgca tctactacta gatatagatg attaaaatca
     5101 ggaaatacac cttgaacata ttgcggcaat gacatcatta ttttcgtgcc atatccaaaa
     5161 ccttgatatt tttcattaat cgataatgaa cgaatataaa cgacattttc aggtgtatca
     5221 taaccttcat gctgatagta acgatgtaat acaaaaaagc ccaccacttc atttttatcg
     5281 tttattgcta cgttagcaat cctatcagca tcattaattg catcatcaat aacttcttta
     5341 ggtaaagatg aatatatttg ttgtctttca ttcaagtcaa aatcattaat tgctgaacga
     5401 tattgttcat caaatgcttt taaagtgata ccttcaaaac aaagttcttt agccatatat
     5461 ttaaccccta tctttttata tatgaagcat gaatccccat attattttac ctattatttc
     5521 actacgatac cattatttgc tctaagttaa ataaattact tatttatatt ttttaaattt
     5581 caaaattaat gtactattcc tcattactta tcattttaat cagagactta caaatacact
     5641 gcgtacgaac tacttttaat attgcgtcac taactaacaa tcctatacaa cttcaaaatt
     5701 cctagaaaat aatatatata acttaacgtc cacgtttgta aaacattaat tgcgatacgc
     5761 ttacaatgtt gaaatgcaat ggcatcaaat ttgttaataa ttactattat tacttgtttt
     5821 ttagtcattt tactatacta ttatttaagg atagacatta ataatgaggt gggaatctta
     5881 tgaaagttgg cattgacgct ggcggtacac taattaaaat cgttcaagag caagataacc
     5941 aacgtacttt taaaactgaa ttaactaaaa atattgatca agttgtggaa tggttaaacc
     6001 aacagcaaat tgaaaaatta tgcttaactg gaggtaatgc aggtgtcatt gctgaaaaca
     6061 tcaacattcc tgcacaaatt tttgtagagt ttgatgctgc atctcaagga ctaggtattt
     6121 tgttaaaaga gcaaggccat gacttagcag attatatttt tgcaaatgtt ggtaccggta
     6181 cgtcactaca ttatttcgac ggccaatcac aacgtcgtgt aggcggtatc ggtacaggtg
     6241 gaggcatgat tcaaggactc ggttatttac taagtcaaat cactgattat aaacagttaa
     6301 ctgacatggc acaacacggt gatcgtaaca ctatcgattt aaaggttaga catatttata
     6361 aagataccga accacccatt cccggtgatt taacagcagc taattttggt catgtactac
     6421 atcatttaga tgctgatttt acacctagca ataaattagc agccgttatt ggtgtcgttg
     6481 gcgaagttgt tacgacaatg gccattactg ttgcacgtga atttaaaact gaaaatatcg
     6541 tttatatcgg ttcttcattt cataataacg ctttattacg caaagttgtg gaagattata
     6601 ctgtgttacg cggttgcaaa ccgtactatg ttgaaaacgg cgcattttca ggtgctattg
     6661 gtgcattata tttagaaaaa tagaaaaaga ccatttatat gcgcgttgtt ttaaataaac
     6721 gctatataaa tggcctttta ttttatggtc taagtgtttt gaatacgtat tgttccattt
     6781 attatcactt aattacttac aactcaattc aattaaaatg taagttcatt agacaatttt
     6841 tgcgctgaaa caattgtacc aaccacgcca gtttttaaat ctatcgcaac ggctttatga
     6901 tcatctctaa gttcatctag ccattctccg aaaacagaac cttcaatcgt aattaaatca
     6961 cactcttcaa aatcttcatc tcgaactttt aacgctctgt ttttaatact ccatacaggc
     7021 ataatatgcg gctttttttc gtcgctatca ttttcactaa tgagaataaa ctgcttacta
     7081 ccttttttag ttaagccgta tacttcatca tatttcgcta catcttggac aaattctttg
     7141 attctaattt tatctaattc cgtcatcact tcttgtgtaa cagctacaac gtttagtaga
     7201 tgcccttctt cttccattgt ttgattcata ataatatggt cttgttcatc aaacaattca
     7261 tctaattcat aagtagcaaa acgatcaacg tccattttta caactttatc aaatggaata
     7321 tcacgacttg ttaaaaatga tacggccaca ctttcttctg tccaaacgcc tacatacgct
     7381 tcacttttat aatttaggcg tactagtttt ttatttttag aggcgaaata aatacattca
     7441 tttattaata tatctctaaa gaatggatat gctttataag acatgcttga ccctc
//
LOCUS       DAKTLH010000126         7385 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.126, whole genome shotgun sequence.
ACCESSION   DAKTLH010000126 DAKTLH010000000
VERSION     DAKTLH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7385
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.126"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            113..1675
                     /locus_tag="O0394_002175"
     CDS             113..1675
                     /locus_tag="O0394_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCY0500243.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGYEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(1686..1793)
                     /locus_tag="O0394_002176"
     CDS             complement(1686..1793)
                     /locus_tag="O0394_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500244.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(1865..2659)
                     /locus_tag="O0394_002177"
     CDS             complement(1865..2659)
                     /locus_tag="O0394_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCY0500245.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNFFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            complement(2679..3755)
                     /locus_tag="O0394_002178"
     CDS             complement(2679..3755)
                     /locus_tag="O0394_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HCY0500246.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
     gene            3939..5408
                     /locus_tag="O0394_002179"
     CDS             3939..5408
                     /locus_tag="O0394_002179"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCY0500247.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLALGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            5401..6222
                     /gene="nadE"
                     /locus_tag="O0394_002180"
     CDS             5401..6222
                     /gene="nadE"
                     /locus_tag="O0394_002180"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCY0500248.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            6493..7095
                     /locus_tag="O0394_002181"
     CDS             6493..7095
                     /locus_tag="O0394_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCY0500249.1"
                     /translation="MSFVTENPWLMVLTIFIINICYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            7076..7249
                     /locus_tag="O0394_002182"
     CDS             7076..7249
                     /locus_tag="O0394_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HCY0500250.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIVFVGKKIQ"
BASE COUNT         2532 a         1064 c         1375 g         2414 t
ORIGIN      
        1 atctgataaa aggggtattt tgacggtaat gataatacaa gatagacaac tttctatact
       61 ctaatatagt gagttgaagt agcttgtcat aatcatcatg agggggaaat ttatggctta
      121 tttcaatcaa catcaatcaa tgatatcgaa aaggtattta acattctttt caaaatcaaa
      181 gaaaaagaaa ccgtttagtg cgggacaact tattggacta atattaggtc cattactttt
      241 cctattaaca ttattattct ttcatccaca agacttacct tggaaaggcg tctatgtttt
      301 agcgattact ttatggattg cgacttggtg gattactgaa gcaattccta ttgcagcaac
      361 gagcttatta ccaattgtgt tattaccatt aggtcatata cttacaccag aacaagtatc
      421 atccgaatat ggcaatgata ttatcttttt gtttttaggt ggatttattt tggcaattgc
      481 aatggaaaga tggaatttac atacgagagt agcgttgaca attattaatt tgattggtgc
      541 aagcacttca aaaatattac ttggattcat ggtggcaaca ggattcttat ctatgtttgt
      601 atcgaacact gcagctgtaa tgattatgat tccgattggt ttagcaatta ttaaggaagc
      661 acatgattta caagaagcca atacgaatca aacaagtatt caaaagtttg aaaaatctct
      721 agttttagca attggctatg caggtacgat tggtggcttg ggtacattaa tcggaacccc
      781 gccattaatt attttaaaag gacaatacat gcaacatttt ggatatgaaa ttagttttgc
      841 taaatggatg attgtaggga ttccaacggt cattgttttg ttaggtatta cttggctcta
      901 tttaagatat gttgcgttta gacatgattt gaaatattta cctggtggtc agacgttaat
      961 taaacaaaag ttagacgagc ttggcaaaat gaagtatgaa gaaaaggtag tacaaactat
     1021 ctttgtactt gctagcttat tatggattac aagagagttt cttctgaaaa aatgggaagt
     1081 tacgtcatct gttgcagatg gtacgattgc tatttttata tcaatattat tatttattat
     1141 tccagctaaa aatactgaaa aacatcgccg tatcattgac tgggaagttg caaaagagct
     1201 cccttggggt gtattaattt tatttggtgg cggtttagca ttagcgaaag gtatttctga
     1261 aagtggttta gcaaaatggt taggcgaaca gttgaaatca ttaaatggtg ttagtccgat
     1321 tcttattgta attgtcataa caatctttgt cttattttta actgaagtga catctaatac
     1381 tgcaactgca acgatgattt taccgatttt agcaacgttg tctgttgctg ttggagtgca
     1441 tccattacta cttatggcac ctgcagctat ggcggctaac tgtgcataca tgttaccagt
     1501 agggacacca ccgaatgcaa ttatctttgg ttctggtaaa atatctatca aacaaatggc
     1561 atcagtagga ttctgggtaa acttaatcag tgcaataatt attattttag tcgtgtatta
     1621 tgtaatgcct atagttttag gtattgatat aaatcaacca ctgccattga aatagtaatt
     1681 gcagattaga acgaaaaata aaaggttaca ttagcaattg cttggacgag tggtaacgaa
     1741 acgtataccg cagcatcgtg taaaaacaat acaaacaaaa gaaagtcaac caaggatgga
     1801 ttcctatttt aatccttggt tgactcttta ttttatttaa attgtagaac ctagaaaata
     1861 aagtttaatt aaaagcacca atcatttcta ctttgaaatc taaggtttct aaaatagcaa
     1921 tgactttctt tatatcggtt gtaattgcag aatcagcctg aacgaaaaat cgatacatac
     1981 ctaattgtgt ttttaaagga cgagactcaa tccaggataa attaatatta aacaaagcaa
     2041 atgtattaag cacacttgct aacaacccag gtttatcatg cattggtgta attaaaaaca
     2101 tcaatgatgt cgcattttga tcaaattgct gctgattttt tataactaaa aaacgtgtca
     2161 cgttatgtgg atagtcttca atatgtgtat caataggtgt aaaaccataa gcttcgccac
     2221 tacctaaagg tgcaattgct gcaacgccat tttcaatttt agtcaaactt tgaattgtac
     2281 tgtcgacata atcatagtca aattgatgtt gatgtatgta atttgttgtt tgactgattg
     2341 ctggtgcaat agaatacact tttttaatat cagaaatgga atctgttcca ttaccatata
     2401 atgcaaagtt aatatctaaa cgtatttcac cgtgtgcaaa gacatcttgc tgtgcaagtg
     2461 catctgccac aatgttgatt gttccttcta tagaattttc aatagggaca acaccaatcg
     2521 atgtgtcatc atctgcaact gccttgatga cttcaaaaaa atttgacttt ggttgaaaag
     2581 ttgcttcatt ttcagaaaaa tactgacgac aagccaaata tgaaaatgta cctttagggc
     2641 ctaaataata taattgcata tgctacacct ctactaactt aatgatggaa agggcactgg
     2701 ttagcatttg attctttctt tttatagaaa aagtttggat cttttactgt attgtcatat
     2761 ccgtgatgat aatttgacgt caatgttgga gataatggcg gtgctagcca agaccatttt
     2821 ccggtaactt gacgaccttg ttgtgcttcg ttacgttcga atagttcgaa ttgctttgca
     2881 gcggtcaaat gatcgacaat tgatacgcct tcttttttaa aggaatgata cacagcatag
     2941 ttcaattcaa caagtgctcg atctttatta aatgaattat ttttaagtgt atcaaattca
     3001 aacgcatctg caactttttc tagtaaattg taacggtaat catcaataaa gttacgtacg
     3061 ccaatttcag ttaccatata ccaaccgtta aagggtgcag ttggatatac aatgccaccg
     3121 atttttaagt ccatattgga aatgataggg actgcatacc attttaagtt caattttctt
     3181 aattttggat aatgattatg ttcaataggt acttctttaa ttaatgaagt aggatattcg
     3241 taaaatttaa ctgactcatt aggtaattgg taaatcagtg gtaacacgtc aaaattagta
     3301 ccttttcctt tccaacctaa gtgatttgct aagcgtgtaa cttctttttc agcaggatca
     3361 ccacaattgt catagccagc atagcgaatt aattgattgt tgaaaatttt aggtccatcc
     3421 tttggagcat atatagtaat atacggcttt aatttacctt catttgtagc ctgtgtaata
     3481 tgataagtaa ttgatgataa gaacgatgct tcgtcagtaa catctcttgc atcaatgaca
     3541 tttaacgaat cccaaaataa acgaccaatg caacgatttg aattacgcca agccatttta
     3601 gcaccataaa taagttcttc ttctgtatgt gtatatgtcc cagtttcttt tatttctagt
     3661 tcaatgtcat gtaaacgttt attgataatt tgcgtttcat aatgacactc tttatacatg
     3721 ttttctatga aagcttgagc ctctttaaat aacattaaca acacctcgct ttatattata
     3781 gtctacatta ttaaaatact cttaaaaatt atgtatatgt cattaaattg ttggttgatt
     3841 ttaattaaaa gtatggaaat taaggggctc ttatgtatat aaaaaaatga attatgataa
     3901 aatgtaagaa aatatttagg tcgattggag agatacaagt gtaccaatta gaagacgaca
     3961 gtttaatgtt acataatgac ttatatcaaa taaatatggc tgaaagttat tggaatgata
     4021 atattcatga aaaaatggct gtatttgatt tgtattttag aaaaatgcca tttaatagtg
     4081 gctatgctgt ttttaatggt ttaaaacgcg tcattgattt tatagaacat tttggatttt
     4141 cagaatctga tttggaatat ttaaagtcta ttggctacaa ggatgatttc ttatcatatt
     4201 taaaagattt aaaattcaca ggcagcatcc gttcgatgca agaaggcgaa ttatgctttg
     4261 gtaacgaacc attgttacgc gtagaagcac cattgattca agcgcaatta atagaaacaa
     4321 ttttattaaa cattgtaaat ttccatacat taattacaac aaaggctagc agaattcgtc
     4381 aaattgcatc aaatgataaa ttaatggagt ttggtacacg tcgtgcgcaa gaaattgatg
     4441 cagcattgtg gggcgctaga gctgcttaca tcgggggctt tgattctaca agtaatgtta
     4501 gggcggggaa attatttggt atacctgtgt ctggtacaca tgcacatgca tttgtccaaa
     4561 cttatggaga cgaatatgtt gccttcaaaa aatatgctga aagacataaa aattgtgtgt
     4621 tcctagtaga tacattccat actttaaaat ctggcgtgcc aaatgcaata aaagttgcaa
     4681 aagaattagg tgacaaaatt aactttgtag gtattcgatt agattctgga gatatcgctt
     4741 atttatctaa agaggcaaga cgtatgcttg atgaagcagg atttactgaa actaaaatta
     4801 tcgcgtctaa tgatttggat gaagaaacga ttacgagttt gaaagcacaa ggtgcaaaag
     4861 tagattcttg gggcgttggt acaaagctga ttacaggata cgatcaacca gcattaggtg
     4921 cagtatataa acttgtagct attgaaaatg aagatggttc atatagtgat cgtattaaat
     4981 tatcaaataa cgctgaaaag gttacgacgc caggtaagaa aaatgtatat cgcattataa
     5041 acaagaaaac aggtaaggca gaaggcgatt atattacttt ggaaaatgaa aatccatacg
     5101 atgaacaacc tttaaaatta ttccatccag tgcatactta taaaatgaaa tttataaaat
     5161 ctttcgaagc cattgatttg catcataata tttatgaaaa tggtaaatta gtatatcaaa
     5221 tgccaacaga agatgaatca cgtgaatatt tagcactagg attacaatct atttgggatg
     5281 aaaataagcg tttcctgaat ccacaagaat atccagtcga tttaagcaag gcatgttggg
     5341 ataataaaca taaacgtatt tttgaagttg cggaacacgt taaggagatg gaagaagata
     5401 atgagtaaat tacaagacgt tattgtacaa gaaatgaaag tgaaaaagcg tatcgatagt
     5461 gctgaagaaa ttatggaatt aaagcaattt ataaaaaatt atgtacaatc acattcattt
     5521 ataaaatctt tagtgttagg tatttcagga ggacaggatt ctacattagt tggaaaacta
     5581 gtacaaatgt ctgttaacga attacgtgaa gaaggcattg attgtacgtt tattgcagtt
     5641 aaattacctt atggagttca aaaagatgct gatgaagttg agcaagcttt gcgattcatt
     5701 gaaccagatg aaatagtaac agtcaatatt aagcctgcag ttgatcaaag tgtgcaatca
     5761 ttaaaagaag ccggtattgt tcttacagat ttccaaaaag gaaatgaaaa agcgcgtgaa
     5821 cgtatgaaag tacaattttc aattgcttca aaccgacaag gtattgtagt aggaacagat
     5881 cattcagctg aaaatataac tgggttttat acgaagtacg gtgatggtgc tgcagatatc
     5941 gcacctatat ttggtttgaa taaacgacaa ggtcgtcaat tattagcgta tcttggtgcg
     6001 ccaaaggaat tatatgaaaa aacgccaact gctgatttag aagatgataa accacagctt
     6061 ccagatgaag atgcattagg tgtaacttat gaggcgattg ataattattt agaaggtaag
     6121 ccagttacgc cagaagaaca aaaagtaatt gaaaatcatt atatacgaaa tgcacacaaa
     6181 cgtgaacttg catatacaag atacacgtgg ccaaaatcct aatttaattt tttcttctaa
     6241 cgtgtgactt aaattaaata tgagttagaa ttaataacat taaaccacat tcagctagac
     6301 tacttcagtg tataaattga aagtgtatga actaaagtaa gtatgttcat ttgagaataa
     6361 atttttattt atgacaaatt cgctatttat ttatgagagt tttcgtacta tattatatta
     6421 atatgcattc attaaggtta ggttgaagca gtttggtatt taaagtgtaa ttgaaagaga
     6481 gtggggcgcc ttatgtcatt cgtaacagaa aatccatggt taatggtact aactatattt
     6541 atcattaaca tttgttatgt aacgttttta acgatgcgaa caattttaac gttgaaaggt
     6601 tatcgttata ttgctgcatc agttagtttt ttagaagtat tagtttatat cgttggttta
     6661 ggtttggtta tgtctaattt agaccatatt caaaatatta ttgcctacgc atttggtttt
     6721 tcaataggta tcattgttgg tatgaaaata gaagaaaaac tggcattagg ttatacagtt
     6781 gtaaatgtaa cttcagcaga atatgagtta gatttaccga atgaacttcg aaatttagga
     6841 tatggcgtta cgcactatgc tgcgtttggt agagatggta gtcgtatggt gatgcaaatt
     6901 ttaacaccaa gaaaatatga acgtaaattg atggatacga taaaaaattt agatccgaaa
     6961 gcatttatca ttgcgtatga acctcgaaac atacatggtg gattctggac taaaggcatt
     7021 cgtcgtagaa agcttaaaga ttatgaacca gaagaactgg aaagtgtagt agaacatgaa
     7081 attcaaagta aatgagaatg aaacaattgc tgattgtttg tcacgaatga aaatgcaagg
     7141 gtatatgccg gtaaaacgta ttgaaaaacc cgtgtttcaa gagcaaaaag atggcacggt
     7201 tgaagtatca catcaagaaa tcgtttttgt aggtaagaaa atccaataac ataatccaat
     7261 ttaaataaag actatttgaa gaggaaaggc tattcaaagt ttgagtaatt ttactttgaa
     7321 tagcctattt gtttatacat gcaagatgct cgatccatat tgtatgagaa acccccagca
     7381 agcta
//
LOCUS       DAKTLH010000127         7214 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.127, whole genome shotgun sequence.
ACCESSION   DAKTLH010000127 DAKTLH010000000
VERSION     DAKTLH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.127"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..1960)
                     /locus_tag="O0394_002183"
     CDS             complement(<1..1960)
                     /locus_tag="O0394_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCY0500251.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGT"
     gene            complement(1984..2955)
                     /locus_tag="O0394_002184"
     CDS             complement(1984..2955)
                     /locus_tag="O0394_002184"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCY0500252.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     NFDESIRHRATSIQLHDKNKLDRYQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            complement(2942..4144)
                     /locus_tag="O0394_002185"
     CDS             complement(2942..4144)
                     /locus_tag="O0394_002185"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCY0500253.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKS
                     LKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            complement(4149..5291)
                     /gene="bshA"
                     /locus_tag="O0394_002186"
     CDS             complement(4149..5291)
                     /gene="bshA"
                     /locus_tag="O0394_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HCY0500254.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            complement(5532..5849)
                     /locus_tag="O0394_002187"
     CDS             complement(5532..5849)
                     /locus_tag="O0394_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCY0500255.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            complement(6186..6866)
                     /locus_tag="O0394_002188"
     CDS             complement(6186..6866)
                     /locus_tag="O0394_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCY0500256.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILIARSSD"
     gene            complement(6938..>7214)
                     /locus_tag="O0394_002189"
     CDS             complement(6938..>7214)
                     /locus_tag="O0394_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HCY0500257.1"
                     /translation="ITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMIWVFLND
                     YIDYFHLQFPYYDFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
BASE COUNT         2310 a         1322 c          935 g         2647 t
ORIGIN      
        1 aagtaccaga tataaaaatg agcgatttaa atttttccaa tacttgtttc gttaatacat
       61 cttttacagc gtaatctttc acatataaac gtattgttga tttttgagat aaatttttaa
      121 tcgaaatgaa actagtatgt ccagctttta aactttgttc gatattttta aatttatctt
      181 ttaaatacaa caactgtttc cgtaatgatt taactgtttt atgactaatg ccattgaata
      241 tttctagcgt tttatttaac ttatcgataa tcgcatgtaa atccttcaaa atgtcttttg
      301 tttcaaaagt aaatacatta tggaagcgat gaatatcatc atcataaaca tcagaatcat
      361 tgataatcgt aaatatcgtt gagaacaatt gctcatttaa ctcatgaatc tcattcatac
      421 tagccttcaa gccaaaaata tcaattggtg caatatctaa tttttccaaa attcgctgct
      481 tttccagttg atcaattgcc tttaacaatt tttcattttc gtttttacca atcaaaccaa
      541 gctgatattt aatatcagca taactcaact catttgtcac ttgatttaag gcatagtctg
      601 gtaagcgatg tgcttcatcc actatacaat catcaaacaa ttgatatatt gaattttcaa
      661 catcagaatg aattaaatgt gcatgatttg taataccaat ttgaatgttc tgtgcatttc
      721 gcttaataaa attataataa tgaacatcgt gacgtgccgg tacatatgtt tcaattttct
      781 ggtcaaaata catcttttga ccacctttta aatttaattc ctgtatatct ccggacggcg
      841 tctctgtaat ccaaatcagc aattgcattt tcaagatatt cacttcgtaa ttacttgtgt
      901 catcttttaa aatttgacta ataagcccca atgaaatgta atcactttta cttttaatca
      961 atagtgcatt aattttaaaa ttcaacgctt cattcattgc tggaatatct ttttctaaca
     1021 attgactttg cagtaattta gtattggtag aaatcatgac atgcttccca gtttcaatat
     1081 tatacatcaa ggccgcaagt aaatatgcta atgatttacc actgcctagt gatgcttcaa
     1141 tcattgcttt ttcactatgc atgagctgat ctaatatagt ttccgctaaa tataattgtt
     1201 gcggtcgata tgttaagcca agttgatcta cagctttgct atataaagac ttcaagctgc
     1261 cattataatt tgttgtcggc tttttaaaat caacttgctt acgatagata atctgttcga
     1321 acttttcgta cgatttatcc aatggctttg catcatattg cctaaccatc tcaaagaaaa
     1381 tatcatacaa atcgtatttc aactgtttac ttaaataata taattgcttc aaagtatcta
     1441 acggtaactt ttcaaatttt tcaaaagcta atatcatcaa tttagcagta gtagcggcat
     1501 cttcgtcagc tcgatgggca tttgctaagg taataccatg tgcctctgct aattcactta
     1561 attgatagct tttatctgta ggaaaagcta ttttaaagat ttctagtgta tctataactt
     1621 ttttgggacg atattgaata ttacaatctt taaatgcctt tttaataaaa ttcaaatcaa
     1681 aatctacatt atgagctaca aaaatgcaat cttttatctt atcgtagatt tcttgtgcaa
     1741 cttgattaaa atatggcgct tgttgtagca tattttcttc aatggatgtt aacgcttgaa
     1801 tgaacggcgg aatctctaaa tttgttctaa tcatagaatg atatgtatca ataatttggt
     1861 tattgcgcac aaacgttata ccaatttgaa tgatatcgtc aaaatctaat tggttgcctg
     1921 ttgtttccaa atccacaacg gcataggttg ccatacccat agctatctct ccttgcttta
     1981 gtgttaaaaa tctatatctg cactaattaa acggtgtgat tcacccgctt catctctaac
     2041 aattagatag ccatcgtaat ctaaatcaat tgcttgtcct ttaaactgtt tatcattttc
     2101 tgtaaatagc aacgttctat tccaaatatt agaagctgca gtatattctt cacgaatttc
     2161 agaaaaaggt aacgttaaaa attgattata tcttttttca atttcttgaa gtaatatctc
     2221 taaaaattga tatctatcta atttattttt atcatgtaat tgtatacttg ttgctctatg
     2281 tctaatactt tcatcaaagt tttctagttg ttgcgtcaaa ttaataccta taccacatat
     2341 tattgcttct ataccatcat tattagcaac catttcagtt aagaaaccac acactttacc
     2401 attatcaata tatatatcat tcggccattt cactttgact tcatcttgac taaaatgttg
     2461 aatcgcatct cttatcccta atgcaataaa taaattaaat ttagatatca ttgagaatgc
     2521 aacgttaggt cttaacacga cagacatcca aagtccttgc ccttttgaag aactccaatg
     2581 tctattaaat cgcccacgac ctttcgtttg ttcatcactc aagataaaaa atgaagattg
     2641 atttccaaca agtgactttt tcgcagcaag ttgtgtagaa tctattgaat cgtatacttc
     2701 actaaaatca aacaaagcag aactttttgt atattggtct attatacctt gataccaaat
     2761 atctgggagc tgttgtaata aatgcccttt atgatttact gaatctattt tacatccctc
     2821 taactttaat tggtcaatca ctttttttac tgcagtgcgt gaaatattaa gtgattccgc
     2881 aatgctttgt ccagatatat aattcggttt atttttatag agtaattgaa gtacatcttg
     2941 actatatttt gacatgatta tccacccatt tcaaaatttc agtttcttcg ttgcttactt
     3001 tacctgttac aatcgctatc tcaatttgtc ttagcacatc ttttaaccac ggaccacttt
     3061 tggcatttaa atgtgccata agtacaccgc cattaaccat catgtctttt ctattatgca
     3121 taggtaaacg atgtaatgtt tcatcaatcg tttgaaggtt aacgattaat ggttcatgtc
     3181 cttgaatatc atttgccttt aacacgtctg ctgcaaccat tacattttta atgagatgcg
     3241 tatcataatc ataaacaaac atttttaatt gttcttttgt aataatactt ggtaatgcat
     3301 tcataatttg aatatattga ttgatatctt ttacttgtcg gttacttagc tttaaaggct
     3361 tcaatgagta attaatatca aatttaactg atactatagc aatcaacaat tctaaatcaa
     3421 ttgcttcagt tacattaatt tgattcatat caagatgttc gaaatacggc atataattaa
     3481 atgctttcag cgattttaaa tgattaaaac tcttttcaac attaatacct cgcattaatt
     3541 tagttagttc aatcactata cgctcaattg ataaaaattt aatatctgcc atttgtatac
     3601 gcatcgcttc gaatgtttcc gttgcaatat caaatgataa ttgtgactgg aaccttaaac
     3661 atcgaatcat acgtaaagca tcttcttgga atcgttcctc agctatacct acagttctta
     3721 ttattcgatt attaatatct tgttgaccat caaaataatc atacaatttg tatgctgtat
     3781 ccattgctat cgcattcatc gtgaaatctc gtcgttgcaa atcttcgtat aaatcacgaa
     3841 caaatgtaac accacttggt ctacggtgat cgacataatc ttcttcagcc cggaatgttg
     3901 tcacttcata attttcatca ttaaaaacta catttatcgt gccatgttct ttacctacag
     3961 gtatcgtatg actaaagata gattctattt catccggcgt tgcacttgtt gtgatatcta
     4021 tatcatgaat atttcttccc atgacataat ctcttacaga gccacctaca taatatgctt
     4081 caaaaccatt gtcttgaatt tgttctaata taggccttgc ctgttcaaat aatgatttat
     4141 ccatattatt actcgccttt acttttgtta tgctcattta gcattttttg ataataatac
     4201 tcatattgat ctgtaataag ttctgatcca aaacgttcag caatatctgc tagcatgttt
     4261 ttctgaagtt tgttgtataa caccttatct tcaagtaatc ggatagcata gtcactcgct
     4321 gaatcacaat cacccacatc tacgacaaat ccagtttcac catgtttaat aacctcttta
     4381 attccaccgg catttgaacc aattggaacg acgcctgttt tcatagcctc aagtaaagtt
     4441 agtccaaagc tttctttttc acttaataat aatactaagt cagataattg gtaaaattca
     4501 cttacgcaat cttgtttccc taaaaataaa acatcctctt ctacgtttaa ctctttcgtc
     4561 aattgacgca ttggcactaa ttcaggacca tctccaagta aaattaattt actaggtatc
     4621 ttttcacgta cttttgcaaa tgtttctata atagtatcta tgcgttttac ttgtctaaaa
     4681 ttcgatacat gtattaacac tttttcatct ggtgctatac caaattgtga ttttaatgct
     4741 gtgttatgtt tagttggaaa ctcattttca cgtacaaaat tataaatcgg tataatttct
     4801 ttgttagttt caataatttc atgtgtttct tgtgctaaag atttactcac acttgtcaca
     4861 atatcacttt tttcaatgcc aaatttaatt gcaccttgga gtgaatgatc atagcccaaa
     4921 acagtaatat cagtaccgtg tagcgttgtc ataattttta tatctttacc tgacatctca
     4981 cgagctaaaa tcccacaaat tgcatgaggt acagcatagt gcatatgcaa caaatcaaga
     5041 tcatattctt taataacttc agcgatttta gtacttaacg taatatcata cggtggatac
     5101 tgaaatactg catattgatt cacttcaact tgatgaaaaa tcatattcgg taatggtttt
     5161 cttattctaa acgggatatt tgaagtgata aaatgtactt cgtgacctcg ctctgctaat
     5221 ttaattccta attctgtggc aataattcca gaaccaccca tggacgggta acatgttata
     5281 cctatcttca ttcgcttgcc catcctttct ttctatttct ctctattatt ctcgatgcgt
     5341 agataattgt ttaaatttaa gtttatagta atgttgagtt tataatttca tatatctaaa
     5401 aacaggtgtt gtatatataa tcattcatct agttatactt actttaaaaa taatataatt
     5461 tcatgcgatg caattcattg atggatgttt ttaatcttaa tcaaatccaa ataaagcata
     5521 tatttttaaa ttcactttct ttcgaatcga tttttatctc ttgtattaaa cttttccatt
     5581 gtttcattaa agctctctgt catatctatt cccattgaat tcgctaaaca taacaacaca
     5641 aataaattat cacctaattc tgctttaatc gtatttgctt cctctgaatc tttcttcttt
     5701 ttttcaccat aggtatgatt tatttcacgt gcaagttcgc ccacttcttc agtcaatcta
     5761 gctaagttag ctaatggtga aaaatatcct gttttaaatt gtccaatata ttcatcaact
     5821 tcacgttgca tttctaccat tgatttcatt tctacgttct ccttatattg catttctaat
     5881 atagtatata tcaatttgaa gtctcatgca tgtttagtgc aatctactga ctctagctaa
     5941 actagatgac aagttgttta ttttagaagt ttaataattc agtttatata aatgtaatgc
     6001 attcctaact aaattaaatc aattgaaatt gggattataa ctttatgata cgtaccacta
     6061 caataaaata atatagtgaa taatctacca ttagaaaaat aagcacaaaa aaactagcaa
     6121 ccacacaaaa atgtgattag ctagttaata agtgtctaat ttaagttaat tgttaatcta
     6181 taagattaat cacttgaacg cgcaatcaaa ataatacgta caagctctgc tacagcgact
     6241 gcagttgctg caacataagt cattgctgct gcagataata ctttacgcgc atgcttgtat
     6301 tctttttcat ttacaatgtt caatgccgta atttgtttca tcgctcttga actcgcatca
     6361 aactcaactg gtaacgtaac aattgagaat aataccgcta atgacattaa accagcacca
     6421 atccataaag cagttgaacc aaatgcacta cctatcgctg ttaagataat acctaacatg
     6481 atgatcatat aacttaatga actccctagg tttgcaacag gtactaatgc tgctctgaat
     6541 cttaagaacc aatatccttg gtgatcttga atggcatgac caacttcgtg ggctgcaatt
     6601 gcagttccag caactgatgg tctgtcatag tttgcaggag atagtgaaac aactttcttt
     6661 ttaggatcgt aatgatctgt taagaatcct tcacctttaa caacttcgac atcataaata
     6721 ccgtttgcat gtaaaatttc taatgcaact tcacgacccg ttttaccact agttgatcta
     6781 acttgtgaat atttctcata gttagattta actttgtgtt gtgcccataa aggaagcacc
     6841 attaatatta cgaaataaat tatcatagta aaaattgaag acaataaact cactctcctt
     6901 tataaatatt ttactgtcat ttgccgtttt tatcaaatca tttacacttt aataatttgt
     6961 ttaattcaat ataaagcaaa agtccaaaaa cacttagaca acatgataat acaccaattt
     7021 gccacacatg tgtagttata aaatcataat atggaaattg aaggtgaaaa tagtcaatat
     7081 aatcattcaa aaacacccaa atcatcgcta cactgattcc aatcatagaa cgtttaaacc
     7141 taggatagaa gtaaattgcc tgaacagcca ttatactgtg ggaaaacatt aataccaaac
     7201 catttactgt aata
//
LOCUS       DAKTLH010000128         7208 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.128, whole genome shotgun sequence.
ACCESSION   DAKTLH010000128 DAKTLH010000000
VERSION     DAKTLH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.128"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..920
                     /locus_tag="O0394_002190"
     CDS             <1..920
                     /locus_tag="O0394_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCY0500258.1"
                     /translation="NKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIMNEF
                     GTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGIAHP
                     LELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMIIINK
                     IDLITDDSLEKIDKQLGMICASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHHGIK
                     SMTYTFTGPIDRHLFYQFIMKLPESVLRLKGYVSFRDQPNAIYEFQYAYGLPDYGIIG
                     MQLPLTIVIIGETLDTNHIRNQLDMLQFT"
     gene            1023..1931
                     /locus_tag="O0394_002191"
     CDS             1023..1931
                     /locus_tag="O0394_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCY0500259.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIVQDIP"
     gene            1966..2253
                     /locus_tag="O0394_002192"
     CDS             1966..2253
                     /locus_tag="O0394_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500260.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYLIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFVKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
     gene            2536..4089
                     /locus_tag="O0394_002193"
     CDS             2536..4089
                     /locus_tag="O0394_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HCY0500261.1"
                     /translation="MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAILAGSDLLGTNHALSLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
     gene            4175..5548
                     /locus_tag="O0394_002194"
     CDS             4175..5548
                     /locus_tag="O0394_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCY0500262.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKKDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVTAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYNVFTSFYKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(5703..5987)
                     /locus_tag="O0394_002195"
     CDS             complement(5703..5987)
                     /locus_tag="O0394_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500263.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(5984..6601)
                     /locus_tag="O0394_002196"
     CDS             complement(5984..6601)
                     /locus_tag="O0394_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HCY0500264.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPNKKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLSEWKYF
                     TYLLLGVFLAVYVMILIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVY
                     FFNEPLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            complement(6787..7203)
                     /locus_tag="O0394_002197"
     CDS             complement(6787..7203)
                     /locus_tag="O0394_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002441420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0500265.1"
                     /translation="MQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGACDD
                     VVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSGHL
                     AKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
BASE COUNT         2482 a         1031 c         1272 g         2423 t
ORIGIN      
        1 ataataaaga tgaaaaaata agaatatcca taattaacgg atttttgggt agtggtaaaa
       61 ccacgttact gacacattat attagtgaat tattaaaaaa tgatgagaaa attaaaatca
      121 tcatgaatga attcggtact tttgatattg atagcaatag tatttcaaat gaaattgaag
      181 tccattcatt gattaatggt tgtgtttgtt gcgatcttaa acaagaactt gtctatgaac
      241 taaaagccat tgctttaaaa ggggacgtta atcatgtcat catagaagcg acaggcattg
      301 cgcatccttt ggaattacta gttgcatgtc aagatccgca aatcgttaat ttctttgaaa
      361 agccgattat ttatggtgta ttagatgcga ctcgattttt agaacgtcat caatataccg
      421 aaaatacagt ttcgctgatg gaagatcagt tgaaactaag tgacatgatt attattaata
      481 aaattgatct tataactgat gacagtcttg agaaaattga taagcaatta ggtatgattt
      541 gtgcaagtat tccaacttat aaaacaacct atggaaaagt ttcgttggaa gaattggact
      601 taactgttaa agacagagag atatcgtctc atcatcacca tcatcatggg attaaaagta
      661 tgacttacac gtttacaggt ccgattgatc gtcatttgtt ttatcaattt ataatgaaat
      721 taccggaatc tgttctacgt ttgaaaggtt atgtgtcatt tagagatcaa ccaaatgcaa
      781 tttatgaatt tcaatatgca tatggtttac cagactatgg aataattggc atgcaattac
      841 cattaacgat tgttattatt ggtgaaactt tagatacaaa tcacatacgt aatcaattgg
      901 atatgctaca atttacgtaa atcagaaaat gtgttgtttc aatttaacgg aaatagcaca
      961 ttaatttctt aatactttta ttgtgaatat tttgtgacat tgagggatgg agtggatgag
     1021 atatggaaca aataatgatt aatcactatg ttcatttttc taggcttgta caaggttttt
     1081 ggcgtgcaaa tgaatggaag atgactgcga aagagttaaa ttattttata aatgaattag
     1141 ttgaacgtgg aattacaacg atggatcatg ctgatattta tggagattat caatgtgaat
     1201 cactgtttgg taatgctttg gatttatcac ccgaattaag aaataaaatt caaattgtta
     1261 cgaaatgtgg tatcattttg ccttctaagc aatttgattt tacaaatgga catcgttatg
     1321 atttgagtag taagcacatc gtgaaatctg ttgaacagtc attaatcaat ttgaatgtag
     1381 attatttaga tagtctactc attcatcgtc cttcaccatt gatggatcca gaacaagttg
     1441 ctgatgcatt aactaaactt gttaaacaag gtaagttgaa gtcattcggg gtgtcgaatt
     1501 ttaatcattc acaataccaa ttgttaaatc aatatattat gaaagaaaga ctacatatta
     1561 gcatcaatca attagaatta tcgccatatc acgttgatag tttacaagat ggaacaatgg
     1621 attcaatgta tcaaaaccat gttcaaatca tggcttggag tccttttgca ggcggtaaaa
     1681 ttttcgacaa ggaagatatt aaagcgcaac gtattatgaa agttgttcaa tcaatagctg
     1741 acaaatatgg tgtgagtgac acagctgtga tgatagcgtg gttagtaaaa ataccgcatc
     1801 gtatcatgcc gatacttgga acaagtcagt taaagcgtat tgatcaagca atcgaagggc
     1861 tacaacttaa tttagatgat cagtcgtggt ttgacattta caccgctatt atcgtacaag
     1921 atattccgta aacttattta cttttaaatc ataaaggagc atatcatgac aaacgaagat
     1981 aaacgtttcg aacaattaag atttgaacgc aaatttatag ttattccgta tttaatttat
     2041 gcagtcattg tattactatt aaatattttc tattctgatt tgaaaataac aatgacatta
     2101 ttcggacttt tctttgcgta taatgtagtc attttgttca tagcatttgt taaacattat
     2161 aaacgcacat tgttactaag tcttatatta acagtgctta gtggcgcggc attctttgga
     2221 attatttatg tttatggcat taatcatttt taaacattaa aaagagacta tctacaataa
     2281 tctcatttgc attttatcta actaagttta acttttgctt agaaaaatga tgctatgagg
     2341 atattcagta gatagttttt ttatgatcta atatttaaac ttagatatcg ttttgtaatt
     2401 aaccgagata actcatcacc tagcaatatt atttacaaaa tatttcgata aaattgtgta
     2461 ttttgcataa attaaaaatg tatgttttaa aaaattgtta taattaaatt aagattaaac
     2521 aaaggggtga ctgttatgtc agaagaaaaa catgtagttg aacatgaaca acaaaagaaa
     2581 gaaaagacaa aaaagcaata caagccattt tggattgtca tgagttttat aatacttata
     2641 gttgtactat tactcccggc accttcaagt ctgccgataa tggctaaggc agtactagct
     2701 attttagctt ttgcagttat tatgtgggta acggaagctg tatcatatcc ggtgtcagca
     2761 actttaatta ttggcttaat gatattactt ttaggattta gccctgttca aaatttaggg
     2821 gagaagctag gtaatccgaa aagtggcagt gctattttag ctggaagtga ccttctagga
     2881 actaatcatg cattatcatt agcgtttagt ggatttgcaa cttcagctgt agctctcgtt
     2941 gcagctgcat tatttttggc tgctgctatg caagaaacga atttgcataa aagactagct
     3001 cttttagtgt tatcaattgt tggtaataaa actagaaata tagttattgg agcaattatc
     3061 gtttcaattg tacttgcatt tttcgttcct tctgcaacag ctagagcagg ggcagttgta
     3121 ccaatcttgc tgggtatgat tgcggcattt aaagtttcca aagatagcaa gttagcgtct
     3181 ttattaataa ttacttcagt acaagctgtg tcaatttgga atattggtat caaaacggcg
     3241 gcagcacaaa atatcgtagc gattaatttt ataaaccatc aattaggatt tgatgtttca
     3301 tggggcgagt ggttcttata tgcagcgcct tggtccatag ttatgtccgt agctttatat
     3361 ttcatcatga ttaaagtgat gcctccagaa attaatacaa tagaaggtgg taaagattta
     3421 ataaaagaag aattgcataa acttggcccc gttagcccac gtgaatggcg tttaattgtt
     3481 atatcgatgt tattattact gttttggtca actgaaaaag tattacatcc gattgactct
     3541 gcatccatta ctattattgc tttaggtgtt atgttaatgc cgaaaattgg tgtcatgaca
     3601 tggaaacatg ttgaaaataa aataccatgg ggaacaatta tcgtgtttgg tgtaggtatt
     3661 tcactaggta acgttctttt gaaaacaggt gcagctcaat ggttaagtga tcaaactttt
     3721 ggtgttttag gtttaaaaca tttacctatt atcgcgacaa ttgcgcttat cacgcttttt
     3781 aatatattga ttcatttggg ctttgcgagt gcaacaagtt tatcatcagc gttaatacct
     3841 gtttttattt cgctaacctc tacgttacac ttaggagacc agtctatagg atttgtttta
     3901 attcaacaat ttgttattag ttttggtttc ttattacctg ttagtgcacc tcaaaatatg
     3961 ttggcttatg gcactggtac ttttacggtt aaagatttct tgaaggcagg tataccattg
     4021 acaattgtag ggtatattct agtgatagtt tttagcatga cttattggaa atggttaggt
     4081 ttgctttaat taaaaatata aataagaatc taggttattt taaagtgaca aaaagcttaa
     4141 taaaataaaa agataattga agggtgtttt gtttatggca attgctgtgt tattaaatcg
     4201 aatgtttcga atggaacaca atccattatt tgaatatatt tatcaacaaa aaaaagacat
     4261 tgatgcatgt tattttatca ttccggaaga ggacatgtct tcagcttctg atttgaaagc
     4321 acagttttat cgcggtactt tgcagcgctt ttaccaatcg ttgcacgcag aaaagcttac
     4381 accttatgtt atgtcttatg acgatatcat ttcattttgt aaagaaaaca atatctctga
     4441 agtagtgact gcgggtgata ttatgagtta tcatcttgaa gaatatgata ttttacatca
     4501 acgttcttta ttcaatgaag cacgcattgc cgttactttg atacgtggga atcattactt
     4561 taaagcgagt aaaacaatga atcaacaagg ggagccatac aatgttttta ctagtttcta
     4621 taaaaaatgg cgaccttact tgaggcatag agacgtatat cactatgatt taaaatcatt
     4681 cgaagacttt gtcattgcat cacctgatga tttagtgttt gatgacatag catttggatc
     4741 ctcacaaata attgaacaga ataaatggca acatttttta gatcaagata tacagaatta
     4801 cgaaagcgga agagactatt tacctgaagt attaacaagt cagctaagtg ttgctttagc
     4861 atatggatta ttagatatta ttgaaatttt taatgattta ttggcgcgtt atgatgaaga
     4921 tgaggcaaac tatgaagcat ttatacgtga actcattttt agagaatttt attatgtgtt
     4981 aatgacacag tatcctgaaa cctcatacca agctttcaaa cctaaatatc gacagataaa
     5041 atggtcgcaa aatgaagcgg attttaatgc atggtgcgaa gggcaaacag gatttccaat
     5101 cattgatgca gcaataatgg aattgacaca aactggtttt atgcataatc gaatgagaat
     5161 ggttgtgtcg caatttttaa ccaaagattt atttatagat tggacatggg gagaaaaatt
     5221 ctttagaaag caccttattg actatgatgc agcatcaaat attcatggat ggcaatggtc
     5281 tgcttctaca ggtacggatg cagtgccgta ttttagaatg tttaatccaa taagacagag
     5341 tgaacgcttt gatgctaaag ctttgtatat caaaacatat cttccgattt ttaatcaaat
     5401 tgatgcaaaa tatttgcatg atacacaacg caatgagtcc aacctttttg aacaggggat
     5461 tgaattaggt agtcattatc caagacaaat ggtagatcat caagaaaaac gtacacaagt
     5521 tttagctaca tttaaagcgc tagactaatt cggctcaatt gatagatctg gcatgagtaa
     5581 aatctggagc agaatataaa gttcttaagc aatataaata agccaatttc tattattgat
     5641 gtgatagaaa ttggctttcc tcaaatatat aagttgtatt tagttttctt attaatttga
     5701 tgttatctta gtatgtccgt aaataaagtg aggtatagta cgacatactc taaaaacgta
     5761 gcgagataaa tatatttcaa tctaactttt atgttttgag gcacttgcca tttaggatat
     5821 tgtcgttcgt aatacgacac ttgttgtata aatacaccta gtccaaatgg cagcatcatg
     5881 agtaagatac ttcttaaata acttaaacca atatcatgcc atatgtgtcc aataatcaat
     5941 tgaaagacaa tgatagatac tattaaaacg attatattta ttgtcacttg ttcaaacgca
     6001 ctccttttcc aaataataga attgctgctt gcatgacaac cataaaacat acaaacatag
     6061 cagttttaag cgttagactt tctagaatgt gatttagaac atgtaagggc tcattaaaga
     6121 aataaacgga atgtaagcgt aagaaacgac caatataaat tccgaatcca tttaaaaaca
     6181 ttagcacgac aacaattaat ctattaagcc aacggtgaga agtcaatgtt agtatttcaa
     6241 aatagattaa aatcatcaca taaaccgcta agaagacacc aagcagtaaa taggtaaagt
     6301 atttccactc acttaaattt agtcctgcgt aaaagttgaa ttgaaattgg ttcaaatgga
     6361 ttaaatcagt taccatataa aatgtatttg gtaaaagtaa cacaaatata aaactatata
     6421 tgataaagag tggccattca tactttttat tcggtttgaa taatcgtaat aatagactaa
     6481 gctcaaaagg tatatatgct aaaaacaaat ttaaagtcat aaattgaaaa attttagtct
     6541 caaaaaggga gacgataaat aaaattaaaa agtaaattct agcgatgtat cgagattgca
     6601 tcaataataa acactacttt ctaatgaaaa agttattcat tcaaatgtga tagttataaa
     6661 cgcattcaat tcctaattaa aaatttaatt taactcgttg aagtatatta ataaacgaga
     6721 atgcatttat gattaagcac ttatgataac ttgtaataaa atttgattca actaaaaagt
     6781 aaagtgctat tgaggatcaa gtaaatttaa accgactgca tctttgaatg aacgttgaat
     6841 gtccaaatca gttacagtaa gaatgatttc agcagttgca gaaacaattg ctttagcgag
     6901 atgtccgcta aaagtttcat atgattgagt accgaaagta gcatgcaaat gtgcaaaatg
     6961 accattgtct agacgagaaa tattacctaa taagctcgtc aattccagtg gctcagtaat
     7021 atgtttttct tcgtattgtt tcgttgttaa attgaaaaat tttaatacaa cgtcatcaca
     7081 tgcaccaatg ccgctgacag atgtaaatgt taagtcttgg tcatctgcaa aggttgttat
     7141 acattcaacg atatcttctc ctttttccaa cactagtagt atagtatgat tacttttttg
     7201 caatttca
//
LOCUS       DAKTLH010000129         7083 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.129, whole genome shotgun sequence.
ACCESSION   DAKTLH010000129 DAKTLH010000000
VERSION     DAKTLH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.129"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            67..378
                     /locus_tag="O0394_002198"
     CDS             67..378
                     /locus_tag="O0394_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0500266.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            478..1335
                     /locus_tag="O0394_002199"
     CDS             478..1335
                     /locus_tag="O0394_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HCY0500267.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            1364..1960
                     /locus_tag="O0394_002200"
     CDS             1364..1960
                     /locus_tag="O0394_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCY0500268.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            1981..5805
                     /locus_tag="O0394_002201"
     CDS             1981..5805
                     /locus_tag="O0394_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCY0500269.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEKRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKPKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTDASQLHTNGIENETVSNDENKQASIQNEDTNDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEVDEDTTSNIEDNTNRNASEMHVDAPKTQEY
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLSVNSLKTNDVNDNHVVEDSSMNEIEK
                     NNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAVSERMPASQATPSSRSDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAETENDYQNAQQYSEQKPSVDSTQTEIFEESQDDNQLENEQVDQSTSSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQSSFSNKNEDTANENRPRTNQQDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIESDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            5829..>7083
                     /locus_tag="O0394_002202"
     CDS             5829..>7083
                     /locus_tag="O0394_002202"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCY0500270.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVILFMGAG"
BASE COUNT         2756 a          977 c         1324 g         2026 t
ORIGIN      
        1 aatcgggtaa taacaactat tatctctaaa tagttatata taatcattaa ttaaggagac
       61 ataaaaatga aacaacttga atcagaacaa caatttgaat ctttaaaaca aggtgctaca
      121 gtatttgaat tcactgcagg ctggtgtcca gattgtagag tgatagaacc agatttaccg
      181 gaattagaag cgagatatcc tatgtttgac ttcgtatcag tagaccgtga taaatttatg
      241 gatatttgta ttgaaaatgg tattatgggt attccaagtt ttctagtata taaaaatgga
      301 gaactgcttg gaagttatat tggaaaagaa cgaaaatcaa ttgaacagat agatgcattt
      361 ttagctcaat acgtgtaatt tagactagag aaaaacgggg taatactcgt ttttctctgt
      421 tactatgtgt tgatttattg taaactatta taaggtgcga aattaggagt gttacatatg
      481 aatacctttc aaatgagaga taaattaaag gaacgtttaa gccatttaga cgttgatttt
      541 aaatttaatc gtgaagaaga aactttgcgt atttatcgaa cagataataa caaaggtatc
      601 acgattaaac ttaacgctat agtcgcaaaa tatgaagata aaaaagaaaa aattgtagat
      661 gaaattgttt attacgttga tgaagctatt gcacaaatgg cagataaaac ccttgagagt
      721 atatcatcta gtcaaattat gcctgtcatt agagcgacta gctttgataa aaaaactaaa
      781 caaggtgttc cttttatcta tgatgagcat actgcagaaa cagcagttta ttatgcagtc
      841 gatttaggga aatcatatcg tctaattgac gaaagcatgt tagaggattt gaagttaact
      901 gaacaacaaa taagagaaat gtctctgttt aatgttagaa aattgtcaaa ttcatatacg
      961 actgatgaag taaaaggtaa tattttttat tttattaact caaatgacgg gtatgatgca
     1021 agtaggatac taaatactgc atttttaaat gaaattgagg cacaatgtca aggcgaaatg
     1081 ctcgtagcag tgccacacca agatgtgtta attattgcag atatacgcaa taaaacagga
     1141 tatgatgtga tggcacattt aacaatggaa tttttcacta aaggtctagt tccaattaca
     1201 tcattatcct ttggatataa acagggtcat cttgaaccga tatttatttt aggtaaaaat
     1261 aataaacaaa aaagagatcc aaacgtgatt cagcgtttag aagcaaatcg tcgtaaattt
     1321 aataaagata aatagaaata attggataag gagttttgtc ataatgaatt tattttacaa
     1381 tcctaaatat gtaggagatg tcgcattttt acaaattgaa ccagttgaag gtgaattaaa
     1441 ctacaataaa aaaggtaatg ttgttgaaat tactaatgaa ggtaatgttg taggttataa
     1501 tatttttgaa atttcaaaag atataacaat tgaagaaaaa ggtcatatta aattaactga
     1561 tgaacttgta aatgtattcc aaaagcgtat ttcagaagct ggttttgatt ataaattaaa
     1621 tgctgatcta tcaccgaaat ttgtagttgg ctacgttgaa actaaagaca aacatcctga
     1681 tgcagataaa ttaagtgtac taaatgtaaa cgttggaaat gacacattac aaattgtatg
     1741 tggcgcgcct aacgttgaag ctggacagaa agttgttgtt gctaaagtag gtgcagtgat
     1801 gcctagcggt atggtaatta aagatgctga attacgtggt gttgcctcaa gcggtatgat
     1861 ttgttcaatg aaagaattga atttacctaa tgcacctgaa gaaaaaggta ttatggtatt
     1921 aaatgacagc tatgaaattg gacaagcatt ttttgaataa ttaaggaagg tagtgaaaat
     1981 atgagctggt ttgataaatt attcggcgaa gataatgatt caaatgatga cttgattcat
     2041 agaaagaaaa aaagacgtca agaatcacaa aatatagata acgatcatga ctcattactg
     2101 cctcaaaata atgatattta tagtcgtccg aggggaaaat tccgttttcc tatgagcgta
     2161 gcttatgaaa atgaaaatgt tgaacaatct gcagatacta tttcagatga aaaagaacaa
     2221 taccatcgag actatcgcaa acaaagccac gattctcgtt cacaaaaacg acatcgccgt
     2281 agaagaaatc aaacaactga agaacaaaat tatagtgaaa aacgtgggaa ttctaaaata
     2341 tcacagcaaa gtataaaata taaagatcat tcacattacc atacgaataa gccaggtaca
     2401 tatgtttctg caattaatgg tattgagaag gaaacgcaca agccaaaaac acataatatg
     2461 tattctaata atacaaatca tcgtgctaaa gattcaactc cagattatca caaagaaagt
     2521 ttcaagactt cagaggtacc gtcagctatt tttggcacaa tgaaacctaa aaagttagaa
     2581 aatggtcgta tccctgtaag taaaccttca gaaaaagttg agtcagataa acaaaaatat
     2641 gataaatatg tagctaagac gcaaacgtct caaaataaac aattagaaca agaaaaacaa
     2701 aatgatagtg ttgtcaaaca aggaactgca tctaaatcat ctgatgaaaa tgtatcatca
     2761 acaacaaaat caatgcctaa ttattcaaaa gttgataata ctatcaaaat tgaaaatatt
     2821 tatgcttcac aaattgttga agaaattaga cgtgaacgag aacgtaaagt gcttcaaaag
     2881 cgtcgattta aaaaagcgtt gcaacaaaag cgtgaagaac ataaaaacga agagcaagat
     2941 gcaatacaac gtgcaattga tgaaatgtat gctaaacaag cggaacgcta tgttggtgat
     3001 agttcattaa atgatgatag tgacttaaca gataatagta cagatgctag tcagcttcat
     3061 acaaatggca tagagaatga aactgtatca aatgatgaaa ataaacaagc gtcaatacaa
     3121 aatgaagaca ctaatgacac tcatgtagat gaaagtccat acaattatga ggaagttagt
     3181 ttgaatcaag tatcgacaac aaaacaattg tcagatgatg aagttacggt ttcgaatgta
     3241 acgtctcaac atcaatcagc actacaacat aacgttgaag taaatgataa agatgaacta
     3301 aaaaatcaat ccagattaat tgctgattca gaagaagatg gagcaacgaa taaagaagaa
     3361 tattcaggaa gtcaaatcga tgatgcagaa ttttatgaat taaatgatac agaagtagat
     3421 gaggatacta cttcaaatat cgaagataat accaatagaa acgcgtctga aatgcatgta
     3481 gacgctccta aaacgcaaga gtacgcagta actgaatctc aagtaaataa tatcgataaa
     3541 acggttgata atgaaattga attagcaccg cgtcataaaa aagatgacca aacaaactta
     3601 agtgtcaact cattgaaaac gaatgatgtg aatgataatc atgttgtgga agattcaagc
     3661 atgaatgaaa tagaaaagaa taacgcagaa attacagaaa atgtgcaaaa cgaagcagct
     3721 gaaagtgaac aaaatgtcga agagaaaact attgaaaacg taaatccaaa gaaacagact
     3781 gaaaaggttt caactttaag taaaagacca tttaatgttg tcatgacgcc atctgataaa
     3841 aagcgtatga tggatcgtaa aaagcattca aaagtcaatg tgcctgaatt aaagcctgta
     3901 caaagtaagc aagctgtgag tgaaagaatg cctgcgagtc aagccacacc atcatcaaga
     3961 tctgattcac aagagtcaaa tacaaatgca tataaaacaa ataatatgac atcaaacaat
     4021 gttgagaaca atcaacttat tggtcatgca gaaacagaaa atgattatca aaatgcacaa
     4081 caatattcag agcagaaacc ttctgttgat tcaactcaaa cggaaatatt tgaagaaagt
     4141 caagatgata atcaattgga aaatgagcaa gttgatcaat caacttcgtc ttcagtttca
     4201 gaagtaagcg acataactga agaaagcgaa gaaacaacac atccaaacaa tactagtgga
     4261 caacaagata atgatgatca acaaaaagat ttacagtcat cattttcaaa taaaaatgaa
     4321 gatacagcta atgaaaatag acctcggacg aaccaacaag atgttgcaac aaatcaagct
     4381 gtacaaacat ctaagccgat gattcgtaaa ggcccaaata ttaaattgcc aagtgtttca
     4441 ttactagaag aaccacaagt tattgagtcg gacgaggact ggattacaga taaaaagaaa
     4501 gaactgaatg acgcattatt ttactttaat gtacctgcag aagtacaaga tgtaactgaa
     4561 ggtccaagtg ttacaagatt tgaattatca gttgaaaaag gtgttaaagt ttcaagaatt
     4621 acggcattac aagatgacat taaaatggca ttggcagcga aagatattcg tatagaagcg
     4681 cctattccag gaactagtcg tgttggtatt gaagttccga accaaaatcc aacgacagtc
     4741 aacttacgtt ctattattga atctccaagt tttaaaaatg ctgaatctaa attaacagtt
     4801 gcgatggggt atagaattaa taatgaacca ttacttatgg atattgctaa aacgccacac
     4861 gcactaattg caggtgcaac tggatcaggg aaatcagttt gtatcaatag tattttgatg
     4921 tctttactat ataaaaatca tcctgaggaa ttaagattat tacttatcga tccaaaaatg
     4981 gttgaattag ctccttataa tggtttgcca catttagttg caccggtaat tacagatgtc
     5041 aaagcagcta cacagagttt aaaatgggcc gtagaagaaa tggaacgacg ttataagtta
     5101 tttgcacatt accatgtacg taatataaca gcatttaaca aaaaagcacc atatgatgaa
     5161 agaatgccaa aaattgtcat tgtaattgat gagttggctg atttaatgat gatggctccg
     5221 caagaagttg aacagtctat tgctagaatt gctcaaaaag cgagagcatg tggtattcat
     5281 atgttagtag ctacgcaaag accatctgtc aatgtaatta caggtttaat taaagccaac
     5341 ataccaacaa gaattgcatt tatggtatca tcaagtgtag attcgagaac gatattagac
     5401 agtggtggag cagaacgctt gttaggatat ggcgatatgt tatatcttgg tagcggtatg
     5461 aataaaccga ttagagttca aggtacattt gtttctgatg acgaaattga tgatgttgtt
     5521 gattttatca aacaacaaag agaaccggac tatctatttg aagaaaaaga attgttgaaa
     5581 aaaacacaaa cacaatcaca agatgaatta tttgatgatg tttgtgcatt tatggttaat
     5641 gaaggacata tttcaacatc attaatccaa agacatttcc aaattggcta taatagagca
     5701 gcaagaatta tcgatcaatt agagcaactc ggttatgttt cgagtgctaa tggttcaaaa
     5761 ccaagggatg tttatgttac ggaagcagat ttaaataaag aataattatg agtaaggagt
     5821 tttatataat gacacactat cattttgtcg gaattaaagg ttctggcatg agttcattag
     5881 cacaaatcat gcatgattta ggacatgaag ttcaaggatc ggatattgag aactacgtat
     5941 ttacagaagt tgctcttaga aataagggga taaaaatatt accatttgat gctaataaca
     6001 taaaagaaga tatggtagtt atacaaggta atgcattcgc gagtagccat gaagaaatag
     6061 tacgtgcaca tcaattgaaa ttagatgttg taagttataa tgatttttta ggacagatta
     6121 ttgatcaata tacttcagta gctgtaactg gtgcacatgg taaaacttct acaacaggtt
     6181 tattatcaca tgttatgaat ggtgataaaa agacttcatt tttaattggt gatggcacag
     6241 gtatgggatt gcctgaaagt gattatttcg cttttgaggc atgtgaatat agacgtcact
     6301 ttttaagtta taaacctgat tacgcaatta tgacaaatat tgatttcgat catcctgatt
     6361 attttaaaga tattaatgat gtttttgatg cattccaaga aatggcacat aatgttaaaa
     6421 aaggtattat tgcttggggt gatgatgaac atctacgtaa aattgaagca gatgttccaa
     6481 tttattatta tggatttaaa gattcggatg acatttatgc tcaaaatatt caaattacgg
     6541 ataaaggtac tgcttttgat gtgtatgtgg atggtgagtt ttatgatcac ttcctgtctc
     6601 cacaatatgg tgaccataca gttttaaatg cattagctgt aattgcgatt agttatttag
     6661 agaagctaga tgttacaaat attaaagaag cattagaaac gtttggtggt gttaaacgtc
     6721 gtttcaatga aactacaatt gcaaatcaag ttattgtaga tgattatgca caccatccaa
     6781 gagaaattag tgctacaatt gaaacagcac gaaagaaata tccacataaa gaagttgttg
     6841 cagtatttca accacacact ttctctagaa cacaggcatt tttaaatgaa tttgcagaaa
     6901 gtttaagtaa agcagatcgt gtattcttat gtgaaatttt tggatcaatt agagaaaata
     6961 ctggcgcatt aacgatacaa gatttaattg ataaaattga aggtgcatcg ttaattaatg
     7021 aagattctat taatgtatta gaacaatttg ataatgctgt tattttattt atgggtgcag
     7081 gtg
//
LOCUS       DAKTLH010000130         7064 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.130, whole genome shotgun sequence.
ACCESSION   DAKTLH010000130 DAKTLH010000000
VERSION     DAKTLH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.130"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..457
                     /locus_tag="O0394_002203"
     CDS             <1..457
                     /locus_tag="O0394_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0500271.1"
                     /translation="IDLNEIQNDLNRFINSSASGMYERLIYNENLSNNIMFELFKKDT
                     QDYSDLEEQLIEKFINYVVSNVKKESRSNTLIELSKILENGYTIDKEEFKKEDDYLSK
                     YIFTDIDYLKKLESKINVAIDIRSGEIHLVLDDDSLDILIQNQKYGNEF"
     gene            500..983
                     /locus_tag="O0394_002204"
                     /pseudo
     CDS             500..983
                     /locus_tag="O0394_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837446.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1144..1248
                     /locus_tag="O0394_002205"
     CDS             1144..1248
                     /locus_tag="O0394_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500272.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
     gene            complement(1748..2839)
                     /locus_tag="O0394_002206"
     CDS             complement(1748..2839)
                     /locus_tag="O0394_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0500273.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     AQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLIGIAITTQSIIIIVVLLIKAIITWLLLIPIFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGILLLNLIIILFKTPVGYLISFNTQSLSQSVAENIIRVYTVMTI
                     ILFVVIHAIILMGIVQYYLKRGQKITKDKVKTADKNKKVVTEPKNTKVENDKVTTSVE
                     TKTEKAQDSLNDNTTKTMTSDKPEDNQPK"
     gene            complement(3105..4085)
                     /gene="czrB"
                     /locus_tag="O0394_002207"
     CDS             complement(3105..4085)
                     /gene="czrB"
                     /locus_tag="O0394_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HCY0500274.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH"
     gene            complement(4087..4407)
                     /gene="czrA"
                     /locus_tag="O0394_002208"
     CDS             complement(4087..4407)
                     /gene="czrA"
                     /locus_tag="O0394_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HCY0500275.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            4559..5224
                     /locus_tag="O0394_002209"
     CDS             4559..5224
                     /locus_tag="O0394_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0500276.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGDQDIKDALTQFEN
                     ETD"
     gene            5659..6597
                     /locus_tag="O0394_002210"
     CDS             5659..6597
                     /locus_tag="O0394_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HCY0500277.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            6628..6858
                     /locus_tag="O0394_002211"
     CDS             6628..6858
                     /locus_tag="O0394_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500278.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
BASE COUNT         2513 a          958 c         1253 g         2340 t
ORIGIN      
        1 tatagatttg aacgaaatac aaaacgatct gaatagattt atcaactcta gcgcttctgg
       61 tatgtacgag cgattaatat ataatgaaaa tttatccaat aatattatgt ttgaattatt
      121 taaaaaggac acacaagatt atagtgattt ggaagaacaa ttgattgaaa agttcataaa
      181 ctatgtagtg tctaatgtaa aaaaagaaag taggagtaat actcttatag agttatcaaa
      241 aattttagag aacggatata caattgataa agaagaattt aaaaaagaag atgattatct
      301 ttctaagtac atatttactg acatagatta tttgaaaaag ttagaatcaa aaataaacgt
      361 tgctattgat attcgaagtg gagaaattca tttggtatta gatgatgata gccttgatat
      421 attaatacaa aatcaaaaat acggcaatga gttttgagtc ataactaaat ataatatgtt
      481 gaagagaggt tttatttaaa tggctaaaat tggttatgat cgtgtatcaa cgaaagatta
      541 atatcgatac acttaaagaa tttgggtgtg ggtgtgtata tagtgaaaga ataagtggtg
      601 tcaaacgtac agagcttgtt aagtgtttag attatttacg agagagcgac acattagttg
      661 tctatcaact tgatcggtta ggtagaacga caaaactatt aattgaatta tcacaatgat
      721 tcgatgataa cggaattgac ttacaaatta gtaacatgaa catttcaacg aaagacacaa
      781 tgggcaaaat gttttttacg atgatgagtg catttttcag gattagaagt taatttacta
      841 tgtgagtgta taaaatagac ttagcagcaa caagagcgag aggccgaaaa agcgggcgcc
      901 cctctttacc agagaataaa aaatgagaaa ttaaattttt atatgatgaa caaacgataa
      961 caggggaaga aatagctagt tagacaaggg tatgtcactc aactgtttat cgagttgatt
     1021 aagaaatgaa aaaacttata ctatgaatta ctgtttaaaa gtgtgcatgt tataatattt
     1081 attgagcaag ttggatagat ggtggctaat ctcttaataa aggggtgatg cctatggtta
     1141 tagttgttac tcctagaaag gactagcatg tctgattttg aaatgcttat ggttgtatta
     1201 acaatcattg gtttagtatt gattagtact caagaccata aaaaataacc ttctattcgc
     1261 tttgaccggc atttttgaag gctattttta aataaaatat aaggtcaccg tctttttaac
     1321 gggctcatta gggtaacatg tttccgagtg ttgccctttt tgtgtttcaa gagttaatga
     1381 ttttcatctt ttgcttctac ttactacaaa tatattttaa cacatttttc ttatgaattg
     1441 tagttctgaa cataatcaga attaataaaa ccaactttcc atacagcaga aaatacaatt
     1501 aaaagtatag aatgtaatca cgaattatat aaaaagtatt ggagacctct tcatatatag
     1561 ataattcact tagttatttt agaaagaagc ccctaacaac taaagttgaa aaatagagga
     1621 acacagttgg atgctacgca tcaactgcat aagagcccct aacaactaaa gtttaaaagt
     1681 aggagcgcct gcgctaagcg catgcataag agcccctaac aactaaagtt gtaaggggct
     1741 ctaaaattta ttttggttga ttgtcttctg gtttatctga agtcattgtt tttgttgtat
     1801 tatcatttaa tgaatcttgt gctttttctg tttttgtttc aacagatgtt gtcactttat
     1861 cattttctac ttttgtattt tttggttcag tgacaacttt tttattcttg tcagctgttt
     1921 ttactttatc tttagtaatt ttttgaccac gttttaagta atattgaacg atacccatta
     1981 aaatgattgc atgaataaca acgaataaaa taattgtcat tactgtataa actcttatga
     2041 tattttctgc aacactttga gagagtgatt gcgtattaaa tgaaatcaag taaccaacag
     2101 gtgttttaaa taaaataata attaagttta ataatagtat tccaatgaaa aatttgaaaa
     2161 atgttttttg accatttttc attgctttaa atccattagc taagtgtgtt tttactttat
     2221 cgttagttga ttcaacgaaa ctagtcataa agttgaaaat aggtattaac aataaccaag
     2281 taataattgc tttaatcaat aaaacaacaa tgattatgat gctttgagtt gtaatagcaa
     2341 taccaatcag gtgtgtacta tctgcatatg atgattgtac tgaattcata atcatttctg
     2401 atactggaga aaataatttg tatagtaatg atgtcagaag tgaaagaacg ataatcattg
     2461 ctatagatac aagaccgatc agaacgctct ttaaataatt acctttttta aatgcaataa
     2521 ataaatcggt aaattttact ttttcatgac tcattgctcg tttcataaca tttgtaattc
     2581 cgataaaaat ttgaactaaa acaaacagtg aaactacagc tgctataagt aatacagcga
     2641 taacttttaa atatgcatct actggtggtt tttgcccaaa ttgtgaataa attgcaatag
     2701 tttgtgcata cttagcaagc tggaaattag caagtaaata tacaactgca attacagcaa
     2761 atgctataag tgcatatatc aatgttatta ataattgtgg tttagcattt ttaaatgctg
     2821 atttaaagta agtaaacaaa gtggtgcctc cttttcttca tataaataca ataacaaaaa
     2881 acacgttatt ttcatattaa tatctaataa aaattaatta aagtgactaa agttagagaa
     2941 ttataatagt aaattgtcaa aaatatgact aatgtaagaa ataatagttg aaaatttgtt
     3001 aataaacatt gcatcatcaa agaccaaaca agtcgccata aagacataaa aaacctactg
     3061 ccattgaaaa gtttaatcga tgcaatggca gtaggttaaa attattaatg tacatgcgcg
     3121 tgatgatgag catgatggtt atgtgaatga ctatgtgttc ctgaacatat aatcgattca
     3181 tcatgtttgt gattaggcgt ttctaattga atagtcatat ggtgaatatt taaatgtaat
     3241 aaatcatgct caatgttttc taataataat tcacattctt tcattgtcaa tgtatggtct
     3301 acaacaacat gacaactcaa tgcattcata tcatttgaaa ttgtccaaac atggcaatca
     3361 tgcacacttt gtattcgtga atccttttta atagtagtta taacttcatc tatatcaaca
     3421 tcacttggtg tgccttccat taaaatgtta attgaagatt ttgtgatacc ccaagcactt
     3481 tttaaaataa taacggaaac taaaatactt gcgataggat cggcgattgt ccatccaaat
     3541 gcccaaatta aaatagctgc agtaatggcg ccaactgaac ctaataagtc tccgataaca
     3601 tgtagaaaag caccacgcat atttaaattg tgtgaagtgt cgccgccttt aaacataaag
     3661 aatgcaacaa cgatattgac aattaaaccg ataatactaa tgattaacat ttcttttgat
     3721 tgaacttcag aaggaacaaa gaaacgttta atcgcttcaa aaacaatcaa aatacttatt
     3781 acaaaaagcg ttacaccgtt aaataacgct gcgagtactt cgaaacgttt ataaccaaat
     3841 gtttttgtag ttgtggcatt cttttcagca taaataaatg cgacaagtgc aacacctaat
     3901 gagaatgtgt cgctaaacat atggataccg tcagatagta atgccaagct gttagcaagg
     3961 agaccgccga tgatttcgat aaacatatat agaccgatta ttaaaaacga tataaacaat
     4021 actttcttat tatttgtagt tacatgacta tgcatatggt catgatgatg atgtgaatga
     4081 gacatattat aacccacttt ctttaggatg attcgcgtga tgtatggctt gctttaacat
     4141 agttgctacg tggatgtcat ctaatgaata aatcattgat tggccttgtc gttttgcttt
     4201 cacaagatgc acacttttaa gtaattttaa ttggtgcgag acatttgatt gagataaatt
     4261 caattgatgt gaaatgtgac caacacttgc ttcgctgact gataacaatt ccatgatacg
     4321 tattcgattg taatcgccta atgccttgaa aatttcagtt acgcgttcta atgtatctgt
     4381 atttatttct gaatattgtt ctgacattat atgacaatcc tttcatgtga atatttgttc
     4441 atatattaat ttaatacgtt cccagtagta tgtcaattga tttttatagt tgtaacaagt
     4501 tttacatatc tcataaagtg gtatggcata gagaataata aataggagag tgttgtaaat
     4561 gaatattttg gttataggtg ctaatggcgg tgtaggttcg ttactagtgc aacaattagc
     4621 aaaagaaaat gtaccattta ctgctggtgt taggcaatca gatcaactta atgcgttaaa
     4681 atcacaaggt atgaaagcaa ttcttgttga tgttgaaaat gattcaatag agactttaac
     4741 tgagacgttt aaaccattcg ataaagttat cttttcagta ggttctggag ggaacacggg
     4801 tgcagataaa acaattattg tcgatttaga tggtgctgtt aaatcaatga ttgcgagtaa
     4861 agaggccaat attaaacact atgtcatggt ttcaacatat gattcgagac gtcaggcttt
     4921 tgatgatagc ggtgatttaa agccatatac aatagctaaa cattacgctg atgactattt
     4981 aagacgttca ggtttgaatt atacaatttt acatccaggg gcacttacaa atgcagcagg
     5041 atctggaaag atagaagctg cacaatattt tgatggtaaa ggtgaaattc ctagagaaga
     5101 tgttgcgaca gtattaaaag aaattgtcac atccaatcat tttaatcacc aagagtttca
     5161 aattattagt ggtgatcagg acattaaaga tgctttaact caatttgaaa atgaaacgga
     5221 ttaataagga tagtaaatat gatttgaagc gggttacttt ttcgtaactc gttatttttt
     5281 gaaaatgatg aattataagt atgaataagt acgtattgtc tatgcgtttt acaaagaatt
     5341 tgaacaatgt atggcacaac tattaaagtt gtttattaat tggtataatg ggcgagtaaa
     5401 tttaaaggga aatatgtaca aatgtaagag agatataatc tctatgctat aaatttttga
     5461 aaatgttaaa gtactgtaaa tttaagtgaa gcgctttaat tggcagtact gcaatgttag
     5521 taaatatggt acaatcacta atagttaatt tgaaatgtgg aagttaataa tacgatgtaa
     5581 atgagaacaa aatcaaaata taatggaaca acaacatcag agttctattg cttacttaat
     5641 cacaatggag gtgcaacaat gccattattt ttacaaccaa ttttaaaaac aaaattatgg
     5701 ggcggtcaac gtctaagtga gtttggatat caattagaca atgatacaac tggggaatgt
     5761 tggtgtgtgt cagcacatcc aaatggtacg agcgagatta ttaatggacc atatcaaggt
     5821 caaacattag accgtatttg gtcagaacat cgtgaattgt ttggtgattt cccaagcaaa
     5881 gattttccgc ttctaactaa aatagtggat gcaagagaat cactttctat tcatgtgcac
     5941 cctgataatt cttatgctta tgagcatgaa aacgggcaat atggcaaatc tgaatgttgg
     6001 tatattatag atgcagaaga agatgcagaa atagttatag ggacattagc agagtctaga
     6061 gaagaagttg cgaatcatgt tcaacacgga acgatagagt cgatacttag atatattaaa
     6121 gtaaaacctg gagaattcta ttttattcca gcaggaacag tacatactat ttcttcagga
     6181 atattagcat acgaaacgat gcaatcgtca gacattacat atagacttta tgatttcaat
     6241 cgtcaagata atcaatataa tgatagaccg ttaaatattg aaaaagcttt agacgttatt
     6301 cagtacaatg caccattacc taatattttg cctgaaagcg aaattattga aaaccataag
     6361 tgtacacaca ttgtatcgaa tgatttcttt acattggtta aatgggaaat ttctggcacg
     6421 ttaaattata tgaagcctag agagttctgt ttagttacag tgttggaagg cgaagggcaa
     6481 atgattgtcg atggtgaaat tttcaaactg actactggta caaactttat tttgacttct
     6541 gaagatttgg atagtgtctt tgaaggtgat ttcacattga tgattagcta tgtgtaatag
     6601 tgtaataaca atttaggagt gaatgatttg aagcaatttc tatatattgc gttagtatgt
     6661 ggtgtgatag caggtcttgg tgctttctta catataccgc agtatccgag catgacaatt
     6721 ccacgtatag tagctatttt aggaattatc agtgctatgt tgacttttaa agacaagcaa
     6781 atcagcgcct cattaaagtt tagcgcattg ttaattaatg tgctgccatt atgcggtacc
     6841 tttgtagctt caaattaatt gtcgatagcg cgtttgaata taatacagtg cattaacaga
     6901 agaaatatga ttgtgttaat gacattgatg aaaataaagc acatcaacat aacctctcac
     6961 ttgcatagta gtgagggggt ttttgttggt ttatataaaa tgaagtaatt aatatttaat
     7021 gaaaattaag acatgataaa attctaacgg cagtatagat attt
//
LOCUS       DAKTLH010000131         7053 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.131, whole genome shotgun sequence.
ACCESSION   DAKTLH010000131 DAKTLH010000000
VERSION     DAKTLH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.131"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(205..765)
                     /gene="icaR"
                     /locus_tag="O0394_002212"
     CDS             complement(205..765)
                     /gene="icaR"
                     /locus_tag="O0394_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HCY0500279.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
     gene            929..2167
                     /gene="pgaC"
                     /locus_tag="O0394_002213"
     CDS             929..2167
                     /gene="pgaC"
                     /locus_tag="O0394_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HCY0500280.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            2131..2436
                     /gene="icaD"
                     /locus_tag="O0394_002214"
     CDS             2131..2436
                     /gene="icaD"
                     /locus_tag="O0394_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HCY0500281.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            2433..3305
                     /gene="icaB"
                     /locus_tag="O0394_002215"
     CDS             2433..3305
                     /gene="icaB"
                     /locus_tag="O0394_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HCY0500282.1"
                     /translation="MKYRKFIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLMKASEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            3292..4344
                     /gene="icaC"
                     /locus_tag="O0394_002216"
     CDS             3292..4344
                     /gene="icaC"
                     /locus_tag="O0394_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HCY0500283.1"
                     /translation="MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDSSFNKQFIENVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQ
                     SFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVI
                     MIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQM
                     ISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIF
                     RFIIGKQPYKLNINAY"
     gene            complement(4791..6833)
                     /gene="lip1"
                     /locus_tag="O0394_002217"
     CDS             complement(4791..6833)
                     /gene="lip1"
                     /locus_tag="O0394_002217"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HCY0500284.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKKSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEAD
                     NSQDSNANNNLPSQSRIKEAPSLNKLDQTSQREIVNETEIEKVQPQQNNQANDKITNY
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKDDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGNREEIEYQKKHGGEISPLFKGNHDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTKATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKLTDTKQA"
BASE COUNT         2216 a         1140 c         1054 g         2643 t
ORIGIN      
        1 aataatatta tcaaatcatt gaacaaatcg tattttgcaa caattgatat ttatattaat
       61 gtattgcatt taatttataa aattcatata catcttaata ttctcaatat cgatttgtat
      121 tgtcaacttt atatagattt aaaaaaataa tctcatgtct ttttttacaa aagtaagtta
      181 attattacaa actagtaaca aaaattattt cttcaaaaat atatttagta gcgaatacac
      241 ttcatctttg aattgacttt tactttcttc cactgctcca aatttttgcg aaaaggatgc
      301 tttcaaatac caactttcaa gaaacagcaa tattaaattc tgaaagtctt cttttgtcat
      361 ctttatcttt gattcatcat agaattttgc tatctcttta cttaatgatt gatttaaatc
      421 ttgtatttgt ccgtaaatat ttccagaaaa ttcctcaggc gtattagata attgaacgta
      481 cattctaata tacctttctt cgatgtcgaa aataaactca aataagaatt gatataaagc
      541 atcaattgaa tagttcgatt tattttgatt catcataata atattattaa ggtaatcaaa
      601 acaacattta acactttgtt cgtaaatact ttttttcgag tcaaaatggt aatataaact
      661 cgctttcttt atatttacac ttttagctat atcatcaagt gttgtaccgt catacccctt
      721 ctctgaaaat aaggttattg cgttatcaat aatcttatcc ttcaattttt ataaccccct
      781 actgaaaatt aatcacacta tgttacagga aaattaagtt gcaattacaa atatttccgt
      841 ttaattataa caacaatcta ttgcaaatta aaatactatc aattaccata tggcttacaa
      901 cctaactaac gaaaggtagg taaagaaatt gcaatttttt aactttttgc ttttttatcc
      961 tgtatttatg tctatttact ggattgtcgg ttcaatttat ttctatttta ccagagaaat
     1021 tagatattca ttgaacaaga agcctgacat aaatgtggat gaattagaag gcattacatt
     1081 tttacttgcc tgttataacg aaagtgaaac gattgaagat acgttgtcta atgttcttgc
     1141 actcaaatac gagaagaaag aaattattat cattaatgat ggaagttcag ataatacagc
     1201 agaactcatc tataaaatca aagaaaataa tgactttatt ttcgtcgatt tacaagaaaa
     1261 cagaggtaaa gccaacgcac tcaatcaagg cattaaacag gcttcatatg attatgtaat
     1321 gtgcttggat gcagatacta tcgttgatca agatgcacca tattatatga ttgagaattt
     1381 caaacatgat ccaaaacttg gtgcagttac aggtaatcct agaattcgaa ataagagttc
     1441 tattttaggt aaaattcaaa cgatagaata tgcaagttta attggctgta ttaagcgaag
     1501 tcagacactt gctggcgcag tcaatactat ttcgggtgtc ttcactctat ttaaaaaaag
     1561 tgcagttgtc gacgttggct actgggatac tgatatgatt accgaagata ttgcagtttc
     1621 ttggaaattg catttacgtg gatatcgtat taagtatgaa ccgcttgcca tgtgttggat
     1681 gttggttcca gaaacattgg gaggtctttg gaagcaacgc gtgagatggg ctcaaggggg
     1741 acacgaagta ttactacgag acttttttag cacaatgaaa acgaaaaggt ttcctttata
     1801 tattttgatg tttgagcaaa tcatctcgat tttatgggta tatatagtgc ttctatattt
     1861 aggctatttg ttcataacag caaacttctt agactataca tttatgacat atagtttttc
     1921 aatatttcta ctatcatcat ttactatgac ttttataaac gttattcaat ttacagtcgc
     1981 actctttatt gatagtcgct acgagaaaaa gaatatggct ggactcatat ttgtaagttg
     2041 gtatccgaca gtatactgga ttattaacgc agcagtagtt cttgtcgcat ttccaaaagc
     2101 attaaaacgt aagaaaggtg gttacgcaac atggtcaagc ccagacagag ggaataccca
     2161 acgctaaaat catcgctaaa tattgtaaga gaaacagcac ttatcgctat atcttgtgtc
     2221 ttttggatat attgtttagt tgttctactc gtttatattg gtactatatt tgaaattcat
     2281 gacgaaagta tcaatacaat acgtgttgct ttaaacattg aaaatactga aattttagat
     2341 atatttgaaa ctatgggcat tttcgcgatt atcatttttg tattttttac aattagcata
     2401 ttgattcaaa aatggcagag aggaagagaa tcgtgaagta tagaaaattt ataattttag
     2461 tgttgagtat cttgatcata ttgcctgtaa gcacactgga tggtcatcat attgcaaatg
     2521 cagatgacga ttcacctaaa aaactgaaat ataaagaaaa tagtgctctg gcattaaatt
     2581 atcaccgtgt aagaaaagcg aattttctga ataattttat ttacttcttt tctagtagta
     2641 aagaaattaa aaattatagt gttagtcaat cacaatttga atctcaaata aaatggctaa
     2701 aatcacatga tgctaaattt ttaaccttga aagaattttt atattacaag aaaaaaggta
     2761 agtttccaaa acgaagtgta tggattaact ttgatgatat ggatgaaact atttatgaaa
     2821 atgcttatcc aatcttaaaa aaatataaaa taccggcaac tgggtttatt atcacaggtc
     2881 atgttgggga agaaaacttt cacaacctcg atatgattag taaaaaagaa ctaaaagaaa
     2941 tgtataaaac tgggttatgg gaatttgaaa cacataccca cgatttgcat aacttatcta
     3001 aaaataataa gtcaaaatta atgaaagctt ctgaagctac aatcataaaa gatttaaaca
     3061 aaagtgaaaa atatctaact aaaaacttta aaaagtcgca gaaaactata gcctatcctt
     3121 atggcttgat gaatgacgat aaattaccgg taatcaaaaa agctgggtta aaatacggtt
     3181 tttcattaga ggaaaaagca gtcactccga actccaatga ttattacatc cctagaatat
     3241 taattagtga tgatgctttt gagcatttaa ttaagagatg ggacggattc catgaaaaag
     3301 attagacttg aactcgtata tttacgtgct attatatgtg caattattat tatcacacat
     3361 ttacttacac aaattacttt aaaacatgaa aatatggagg gtggatcctt agtgttacaa
     3421 ttttacattc gtaatattgt gatttttggt acaccttgct ttattatctt gtcacagtta
     3481 ctgacaacct tgaattacca aaaagtcacc tatagatact taactacacg cgtaaaatat
     3541 atacttattc cttacatatt aatgggattg ttttacagtt atagtgaatc attattaaca
     3601 gattcaagtt tcaataaaca attcattgaa aatgtcctat taggtcaatg gtatggctat
     3661 tttatcgttg ttatcatgca attctttatt ttgagttata tcatttttaa aattaactat
     3721 aacctattca acagtaaaat attattattg ttatctttta ttttacagca atcattttta
     3781 tattacttta cgaacaacac agcgtttcac gataccgtgc tacactatta tccattaagt
     3841 gaaaatacta taatattcgg atggattttt tatttcttct taggtgcata tatgggttat
     3901 aactacgaac gtgtattaaa tttcttagaa cgttatttag ttattatgat tgtattagct
     3961 gtagctactt attttgtgtt tattgcgtta gcaaatggag actattggaa cgttaccagc
     4021 ttttcatatt cattaacacc atataatagt attatgttta ttgttatctt gggtatttgc
     4081 acgcatttta aaacaatgtt atttaatacg attcaaatga ttagtgcttt ctcattcttt
     4141 atttatttat tacatccaat cattctagac tcattgtttg catatacaaa tatatttgag
     4201 gataatacaa tggtctttct agcgatatca ctactattca ttttaggatt atgtataggt
     4261 gtcggcatga tattgcgtga attctatatc tttaggttta ttattggaaa acaaccatat
     4321 aaattgaaca ttaatgctta ttaattatta agctatgtta aaaacacgcg gtgggcgaaa
     4381 tcagtttgaa ttgactgact tcgttttacc gcgtgtttaa tattgttata catatattct
     4441 aattgcacat ttaaacttcg taaatgccaa tgggagtggg acagaaatga tattttcgca
     4501 aaatttattt cgtcgtccca ccccaacttg cacattattg taacctgact ttccgccagc
     4561 ttctatgttg gggccccgcc aacttgcaca ttattgtaag ctgactttcc gccagcttct
     4621 ttgttggggc cccgccaact tgcattgttt gtagaatttc ttttcgaaat tctttatgtt
     4681 ggggcctcgc caatgtttta cttgaataat tcttttagaa ttctaaataa tgatccgatt
     4741 aattgaaaga agtctgcagt cattattaat tcctcccttt actttataaa ttatgcttgc
     4801 ttagtatcag tcagcttttc agttttcact aaatcgtctg ctaaatgatg ccaaaaatct
     4861 tgtaattctt ctcttgtgcg cactgtatca gaactgtctt gtcctacaaa gtcaacatga
     4921 tcccaatcat gttttgtagg cgtcacttgc caaatgcctt tttgaatttt atctgtcgct
     4981 tttgtataag cttgattaaa tggatgttga gaagaaataa cggatactaa accatcgttt
     5041 tctcgccatt ctttttcagt agctttaccg attaagttac cagtaatcac aaatgggaaa
     5101 aacatattta agtctgcttt ttgtctatcg ctatttaatg ctttgtgcgt tgcttcacca
     5161 gtgtatgttt tatacacaat gttagggttc aacgacgttt tacgatttaa atctgttgca
     5221 ccctcacgcg tcagatcgta aaatccatta tcttttgatt tccataaatt agattgttta
     5281 acgcgtttga cataatcaat atatgattca tttggcttct gttttagacc ccattgagcc
     5341 aacccgaagt ctactcttga atttttatta ccaaacattt taccgatatc aaatacgatt
     5401 tgtctcacta aagcttcatt accagctaaa tctgatgcgt gtgtaccatt atgtggtgtt
     5461 cctaaagtag taattgatga aatcatattg tcatgattac ctttgaatag tggagaaatt
     5521 tcgccaccat gtttcttttg atactctatt tcttcacgat taccattacg cagtaattct
     5581 tctagttgac gtatcgtttg accgcccata ctatgtccaa ctaggtgtac cttctgtcct
     5641 ggtttccagt ctttgtaaat tccttcgtat gtttttccat aacgttcatg tccatatttt
     5701 gctgcatgtg ctgcaccata atctacacga ccgcctttga tataataata aagttcaact
     5761 gcgcggtcat agttacttcc aaaagcactt atacttgctt cataagcttt gtaaccattt
     5821 tcttctaaat cttggcgaat gttcatttta ttaccgcccc aataatgagc taacactgaa
     5881 ggattaatat catctgtaaa cccattgaaa ccatgcacta aaacgatagg atcctgattt
     5941 ttatactgcc cttgtttggc aactttattc gtttgatcat ctttcgcttt tgcagtaagt
     6001 tcctttgtcc ctttgtttgg taacgttgct actgcactac tttttaataa atttaatcca
     6061 tttttcttgt cattgtcctt tgttggttct actggtgatt tttgattgtt ttttaaatct
     6121 gaaactgata gtgttttttc gtctttttga ggtttcactt cttgttcatt gttaaaattg
     6181 tagttagtaa ttttatcatt cgcttgatta ttttgttgtg gttgtacttt ctctatttct
     6241 gtctcattaa caatttctcg ttgacttgtt tgatctaact tatttaatga tggtgcttcc
     6301 tttattcgac tttgtgaagg tagattgtta ttagcgtttg aatcttgact attatctgcc
     6361 tcttttgact gttgctgtga cgccacttta ctttgtttac catcattaat agcctgttct
     6421 aacccttgat ttacatcgct aggattagca ttgttatttt ttgttgtatc acgttgaata
     6481 tttttattat taagcgatga ttttttatca tcctttagtt gatctttatt caaatcatct
     6541 gttttacgat gattttcttc tgatattgtt ttatcattat gcaagttctt agttaaattt
     6601 tcagtatgtt gctgttcgtc aacaccgttt tcacgttgat aattagcatt ttccctagtt
     6661 tgttgatccc caatagattg tgctgtaact gtatcctcct gtgaatttcc catattcact
     6721 tgcttctcag ctgcttgtgc ttgtccacca cttaaaaata gtaatgtagc tattaaaatg
     6781 gaagatgcac ctacactaaa tttacgaata ctatacttat tttgactttt catcattgtc
     6841 agcacctcta tttttttatt ttagataaac aaattgataa ttattcgagg aataaactca
     6901 aataatttac ttaaccaagc actcataatg accatccttt ctattgttta atttgacgag
     6961 aaagcgttta catttgtatt taaaaaatat attctccaaa gagaatttaa agtgtatacg
     7021 ctaatcattt caaattatta attaatatta tat
//
LOCUS       DAKTLH010000132         7044 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.132, whole genome shotgun sequence.
ACCESSION   DAKTLH010000132 DAKTLH010000000
VERSION     DAKTLH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.132"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            196..1065
                     /locus_tag="O0394_002218"
     CDS             196..1065
                     /locus_tag="O0394_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0500285.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIIMVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
     gene            1348..2349
                     /locus_tag="O0394_002219"
     CDS             1348..2349
                     /locus_tag="O0394_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCY0500286.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            2583..3584
                     /locus_tag="O0394_002220"
     CDS             2583..3584
                     /locus_tag="O0394_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCY0500287.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISTNPAENEGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEIPLVDYIF
                     CTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTP
                     DMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVINI"
     gene            3891..5453
                     /locus_tag="O0394_002221"
     CDS             3891..5453
                     /locus_tag="O0394_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCY0500288.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPTGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLIKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
     gene            5628..6164
                     /gene="amaP"
                     /locus_tag="O0394_002222"
     CDS             5628..6164
                     /gene="amaP"
                     /locus_tag="O0394_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCY0500289.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            6177..6416
                     /locus_tag="O0394_002223"
     CDS             6177..6416
                     /locus_tag="O0394_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCY0500290.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            6479..6988
                     /locus_tag="O0394_002224"
     CDS             6479..6988
                     /locus_tag="O0394_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCY0500291.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
BASE COUNT         2479 a         1002 c         1345 g         2218 t
ORIGIN      
        1 atgcatatac atatttatat attaatcaat gggtgacaca ccattgaaag ctaaagaagc
       61 aatcaattaa caaacgttaa cggacaactt tccaaagtca tcttaaaagt taatgatgta
      121 ttcaaaatca actaaaggac atttttaatc acatccttta tacttatata aaagacatga
      181 atgggagtta aaagtgtgaa gaaaattttt gtaattatta cgacattgtt agcggtcgct
      241 attattatag gctctattat tatggtggtg tttagtcaac gacaggcaca aacatttaaa
      301 atacaacaac agcaatttgt taaaaagcca attccaacat tgttcttaca tggatttggt
      361 ggtagtgcca attctgaaaa gtttatggtg aagcaagcag aaaaaagagg ggtaactaaa
      421 gatatcatca ctgcttacgt ttcaaaggat ggtgcagtga catttaaagg gaaactaaga
      481 aaagatgcag tgaatcctat tgtgaaaatt gaacttgaaa ataacagaca aggctatttg
      541 gataagaatg ctgcgtggtt taaaaatgta ctgactaagt tacaatctga atacaatttc
      601 gataaattca actttgtcgg tcactcaatg gggaacttaa catttgcaca atatatgatg
      661 acatatggta acgacaagtc actgccacag ttaaacaaac aagtgaatat tgcagggaca
      721 tttaatggtg ttttaaatat gaatgaagat gtcaatgaaa ttacagttga taaagatggt
      781 aaaccaagtc gaatgaatca accttatcag caattacgcg tgctaaaaga tatttataaa
      841 ggtaaaggta tcgaagtgct aaatatttat ggggatttga aagatggtac acattcagac
      901 ggcagagtgt ctaatagttc ttcgaaatca ttaaaatatt tactgggaaa tagtccgaaa
      961 agttatcgag aatctaaata tgaaggtgag cctgcacaac atagtcagct tcatgagaat
     1021 gaaaatgttg ctaatgagtt aatcgacttc ttatggaaaa aataaagcaa ctgctagttt
     1081 ttctggggca aaaaagcttg taatattaga gcttttcaaa aattatctat tgtttgagag
     1141 tttccataaa atgtctaagt cgtaacattt taaatttatg attatagcaa atataatgtg
     1201 gttgaagtta catgaatgaa tcagtgctta agtcaaaata taacaactaa gaatgtattt
     1261 gcatacagac atcacgaaga cacaattcaa acttaacgct taagtaacaa ataagtaaaa
     1321 gtttcaaata aagaaaggtg attaatgatg caaaataaac aaattctttt taacaaaata
     1381 ccagaaggca tgcctcaaga ggatacattc aaaattgaag agattgacac gcctaaactt
     1441 gagagcgatg gtgtacttgt acaaacgctg tatatttcag tggatccata tatgagagga
     1501 cgtatgacta aggcagattc gtatgtacag ccttttgaaa ttggtaaacc gattgttagc
     1561 catgtcgttg caaaagtaat tgattcgaca ctcgcagatt ataaaaaagg agatgtcgtt
     1621 gtaggtatgt tgccttggcg aatcattaat catgtccaag cggatcaaat tactaaagtt
     1681 ccaacgacag atgtaccact tgatttatat ttaagtgttt taggaatgcc aggacaaaca
     1741 gcatatcatg gtctgcttga tattggacaa cctaaagccg gtgatactgt tgtggtttca
     1801 gctgcatctg gtgctgtagg ttcagttgta ggtcaaattg ctaagattaa aggttgccgt
     1861 gtagtaggta ttgcgggtgg cgacaaaaaa gtaaattatt taacagagac actgggcttt
     1921 gacgctggta ttgattataa gaaagaggat tttgcagaag cgttagcaca agcagtacca
     1981 gatggtattg atgtttattt tgaaaatgtt ggtggaacag ttggcgatga agtatttaag
     2041 catttaaatc gttttgcacg tgtaccagta tgtggcgcaa tttcgtcata taatcatcct
     2101 gaagccgata tcggaccacg tattcaaggt acgcttatta agaagcaagt aatgatgaga
     2161 ggcttcttgg taagtgaatt cgctaatgct tttaaagaag ctagtgaaca gcttgcaaca
     2221 tgggttcaag aaggtaaaat tcagtcacaa gtaaccattg aagatggttt tgaaaatgcg
     2281 ccacatgcat ttaaaaattt atttactggc gataactttg gtaaacaagt tattaaagta
     2341 acggaataat aaagtatacg ttttaacaaa ctaactttgt taattgacca aaatttaata
     2401 attttttatc taaagtaaat ttgttcagca acatggtaga aataagtcta aaatttaata
     2461 cgaaagtcgt ctgtaattat ttatatattc aaatgaaaaa ggagtcatga tgctgcgata
     2521 taagcacaca tggcttctat tttgtataat aagaaataag ttcataaatg aggtgaaacg
     2581 atatgaaaat gataggattt gagaagccct ttaaattgga agaaggaaat ttatttaaag
     2641 tatacgaaca aagaaagcca acgccagaaa acgatgatat cttagtcaaa gtaaatagca
     2701 ttagtgtaaa tcctgtagat acaaagcaac gacaaatgga agtcactcaa gcacctcgtg
     2761 tacttgggtt tgatgctatt ggtactgttg aagctatcgg accagacgtt acattattta
     2821 gtccgggtga cgttgtattt tatgctggat cgccaaatag acaaggatca aatgcgacat
     2881 atcaattggt gtcagaagct atagttgcaa aagcaccaca caacataagt gctaatgaag
     2941 cggttagtct accgctaact ggtattacgg catatgaaac gttttttgat acatttaaaa
     3001 tttctacgaa tcctgctgaa aacgaaggga agtctgtctt aatcattaat ggtgccggtg
     3061 gtgtaggcag tatagccact caaattgcta aacggtatgg tttaactgtc attacaacag
     3121 catcacgaca agaaacaaca gagtggtgtg aaaagatggg cgcagatatc gtattgaatc
     3181 ataaagagga tttagtgcgt caatttaaag aaattccatt agtagattat atattttgca
     3241 cctataatac agacttgtat tacaatacta tgattgaact gataaaacca ttagggcata
     3301 ttacgactat cgtagctttt aatgaagatc aagatttaaa tgcattaaaa ttaaaaagta
     3361 taacatttac acatgaattt atgtttgcgc gtccaattca tcgaacgcct gatatgatta
     3421 aacaacatga gtatttagaa gatattacga aaaatataga atcagggcat taccaaccaa
     3481 caacgacaca agtttttgaa ggtttatcac ctgaaaactt atatcaagca catcaattgt
     3541 tggaaaaaca atcaatgatt ggaaaattag taattaatat atagattagt aagtaaagtc
     3601 gccttatgca gaaaatactg tataggcgac ttattttttg tgtctagcga ctattgattc
     3661 taaacggaaa tatggcaact ttgcaggtat caaatcaata atgcactaga aaggcattac
     3721 ttaaatacaa taaaatactt tgttgctcaa tgataaataa tttgagtgtg ttttatatat
     3781 catttttaaa agatgaggca ttattaattg tgtctatata aaattacaaa tgattaaaat
     3841 gttgcagtta tgggtatgaa cttaccatta cacagaaagg gtttgtattt atgaaaaata
     3901 aatcagtaag tctagtgttt tgggtatctt tagtgatatg tacgatattt gttgcttttg
     3961 gagcaatatt tccgaaacag ttagaaaaac tcacgcaaaa cattactaca tttattgcgc
     4021 tacatttttc ttggtattat ttattgctcg ttttagttat tttatttgtc tgtgtgtata
     4081 ttttattttc gagatacgca aacattacat taggtgaaga aggcgaagat cctgaattct
     4141 cattgccgtc ttggtttgct atgttattta gtgctggtat gggaatagga cttgtgttct
     4201 ggacaacggc agaaccaatc agtcatgcct tcaaattaac gcctattcat aaagctggca
     4261 cgcaatctgc aattaatgat gcaatgcagt tttcattttt ccattggggg attcatgcat
     4321 gggcagtcta tggtattgtc gcgctagtgt ttgcatattt tagctttcat aaaggatatc
     4381 ctggcttagt cagcgctaca ttgacgcctt tattaggtga aaaggcaatg cgtggacctt
     4441 taggtggtgc cattgatgta ttggcagtca tagctacagt aacaggcgtt gctgcaacat
     4501 taggtttcgg tgcattgcaa ataaacgaag gtttacattt tttatttaat gtaccgagta
     4561 acttcacgat gcaagtaatc ttgattgtca ttgcaaccat attatttact tggtcagcat
     4621 ggtcaggtat cgataaaggt atcaaaacgt tgagtaatat caacatgtta cttgcttttg
     4681 tagtacttat tggtcttttt attgttgggc caacgctata cattttaaat acatttacga
     4741 atggtctagg taattatatt gcaaatttct ttagtatgag tttacgcatt ccgacaggtg
     4801 gtcaaaaatt ccaatggtta caaaattgga caatctttta ctgggcatgg tggatatctt
     4861 gggcaccatt tgtaggtata tttattgcac gtgtgtcaaa aggacgtacg attaaagaat
     4921 ttattctagg tgtacttttt gtcccggcgt tagtttgctt tatattcttt gcggtattcg
     4981 gcgcgtcagc aatttactta caagataatc atattgccga tattgctaaa gctgcgacag
     5041 aaacagcaac ttttgcgacg ctacaacatt atccgctagg ttttgtatta agcttaatta
     5101 ctttacttgt tattatgatt ttctttgtta catcagcgga ttcggcaact tatgttttag
     5161 gtatgttgag ttcaagtggt gatattaatc cgaagtcatt tgtcaaagtg agttggggta
     5221 tcattatggc attatttgca atcatcatga tttacacagg tggtactcaa gcaattcaaa
     5281 atttattaat cattgctgcg ttaccatttt ccgtggtcat tatagcgatg atatggtcgc
     5341 ttataaaatc tttgagtgag gagaaaccga gaaatagtaa taaggtttta attaaacatc
     5401 gtgacccaga tattttagaa tatcgttctt caaaacatat tgacgaaaat taattaataa
     5461 tgaggtttta tttacaaaaa ctagggaata tattactaaa tggtacttca tcttatatat
     5521 taaaaataaa tttaaagtta ttaatttatt atgtttaaga aaaaaactga tgggtacata
     5581 tttaatatgt ttttaaatta actttaacta aggaggtgtc gttaggggtg aaacgactta
     5641 aaaactttat ccttggctta ttaatagtag taatcgtggg gttcctgtta ttcatgtata
     5701 tccaagatgg acgtataact gaatatcaag actacttcct tcaatttgaa tggttccaac
     5761 cattacttat ttcacttgct gcactattga tattaatagg tcttatatta gtatttagta
     5821 tcttcaaacc tacgcatcga aaacctggat tatacaaaga cttcgatgat ggtcatgttt
     5881 acgtatctcg taaagctgtt gaaaagacgg cattcgatac ggttgcaaaa tatgatcaag
     5941 taagacaacc aaatgtagtt gcgaaactat ataacaaaaa aaataaatct tatatcgaca
     6001 ttaaaactga ctttttcgta ccaaataatg ttcaagttca atctttaact gaagcaattc
     6061 gttcggatat taaaaagaat gtagaatatt tcacagaaat gccagtacgt aagttagaag
     6121 ttaatgtgag agatcagaaa acttctggtc cacgagtgtt gtaagggagg tatatcatgg
     6181 ctaacaatca taaccaaaac ggacaagact ctacacaaca agtgattaat tttttaaaag
     6241 tatttaaatg gagaatcgtt ggtttcctag cgtttctatt aatcgcgata ttattcttaa
     6301 cgttaggttt ttggaaaaca gtacttatca tcgttttatg tttaattggt gtaggtattg
     6361 ggtatatgaa agaccgtaag caagatttta tgaatttttt aaatcgatgg agttaattca
     6421 tcaatttaga atttaattaa aaaaatataa aaattaacaa gtaaaaggag aatctattat
     6481 gactgtagat aacaataaag caaaacaagc atacgacaat caaactggtg ttaacgaaaa
     6541 agaaagagaa gagcgtcaaa aacaacaaga acaaaatcag gagcctcaat tcaaaaacaa
     6601 attaacattc tctgatgaag ttgttgaaaa aattgctggt atcgctgcac gtgaagttaa
     6661 aggtatctta gatatgaaag gtggcttaac tgatacattc actaatgcat tctcaagtgg
     6721 caacaatgtt actcaaggtg tatctgttga agttggtgaa aaacaagctg ctgtagactt
     6781 aaaagtaatc ttagaatatg gtgaatcagc accaaaaatc ttccgtaaag taactgaatt
     6841 agttaaagaa caagttaaat atattactgg tttagatgta gttgaagtta acatgcaagt
     6901 tgacgatgta atgactcaaa aagagtggaa acaaaaacac gaaaaaaata acgaaaacaa
     6961 taaccaagaa agacaaggtt tacaataagt tattcgataa aaagggcttg ggacattaaa
     7021 ttcttaggca atgtaaaaaa gctg
//
LOCUS       DAKTLH010000133         6966 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.133, whole genome shotgun sequence.
ACCESSION   DAKTLH010000133 DAKTLH010000000
VERSION     DAKTLH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6966
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.133"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            31..2832
                     /gene="clfA"
                     /locus_tag="O0394_002225"
     CDS             31..2832
                     /gene="clfA"
                     /locus_tag="O0394_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HCY0500292.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSS
                     STNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSL
                     AAVAADAPAAGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLT
                     MPAYIDPENVKKTGNVTLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFV
                     NPENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRST
                     LYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSD
                     NDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSESDSDSESDSDSDSDSDSDSDSDSDSDSDSASDSDSGSDSDSSSDSDSE
                     SDSNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLL
                     ASIGSLLLFRRKKENKDKK"
     gene            3053..4579
                     /gene="vwb"
                     /locus_tag="O0394_002226"
     CDS             3053..4579
                     /gene="vwb"
                     /locus_tag="O0394_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor binding protein Vwb"
                     /protein_id="HCY0500293.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNRASAVVSGEKNPYVSESLKLTN
                     NKNKSRTVEEYKKSLDDLIWSFPNLDNERFDNPEYKEAMKKYQQRFMAEDEALKKFFS
                     EEKKIKNGNTDNLDYLGLSHERYESVFNTLKKQSEEFLKEIEDIKKDNPELKDFNEEE
                     QLKCDLELNKLENQILMLGKTFYQNYRDDVESLYSKLDLIMGYKDEERANKKAVNKRM
                     LENKKEDLETIIDEFFSDIDKTRPNNIPVLEDEKQEEKNHKNMAQLKSDTEAAKSDES
                     KRSKRSKRSLNTQNHKPASQEVSEQQKAEYDKRAEERKARFLDNQKIKKTPVVSLEYD
                     FEHKQRIDNENDKKLVVSAPTKKPTSPTTYTETTTQVPMPTVERQTQQQIIYNAPKQL
                     AGLNGESHDFTTTHQSPTTSNHTHNNVVEFEETSALPGRKSGSLVGISQIDSSHLTER
                     EKRVIKREHVREAQKLVDNYKDTHSYKDRINAQQKVNTLSEGHQKRFNKQINKVYNGK
                     "
     gene            4930..5952
                     /gene="emp"
                     /locus_tag="O0394_002227"
     CDS             4930..5952
                     /gene="emp"
                     /locus_tag="O0394_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HCY0500294.1"
                     /translation="MKKKLLVLTMSTLFATQIMNSNHAKASVTESVDKKFVVPESGIN
                     KIIPAYDEFKNSPKVNVSNLTDNKNFVASEDKLNKIADSSAASKIVDKNFVVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKK
                     VITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGS
                     LPAPRV"
     gene            6287..6757
                     /locus_tag="O0394_002228"
     CDS             6287..6757
                     /locus_tag="O0394_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500295.1"
                     /translation="MKRKVLVLTMGVICATQLWHSNHANALVTESGANDTKQFTEIVS
                     EEKVITVEHAQINIFQSNSNSNLMEFNILTMGGKSGAMVGYSEIDSSHFTDRDKRVIR
                     RDHVKEAQSLVENYKDTQSADARMKAKQKVNTLSKPHQNYFNKQIDKVYNGLQR"
BASE COUNT         2707 a         1093 c         1243 g         1923 t
ORIGIN      
        1 taaaatgact ttttaaaaag agggaataaa atgaatatga agaaaaaaga aaaacacgca
       61 attcggaaaa aatcgattgg cgtggcttca gtgcttgtag gtacgttaat cggttttgga
      121 ctactcagca gtaaagaagc agatgcaagt gaaaatagtg ttacgcaatc tgatagcgca
      181 agtaacgaaa gcaaaagtaa tgattcaagt agcgttagtg ctgcacctaa aacagacgac
      241 acaaacgtga gtgatactaa aacatcgtca aacactaata atggcgaaac gagtgtggcg
      301 caaaatccag cacaacagga aacgacacaa tcatcatcaa caaatgcaac tacggaagaa
      361 acgccggtaa ctggtgaagc tactactacg acaacgaatc aagctaatac accggcaaca
      421 actcaatcaa gcaatacaaa tgcggaggaa ttagtgaatc aaacaagtaa tgaaacgact
      481 tctaatgata ctaatacagt atcatctgta aattcacctc aaaattctac aaatgcggaa
      541 aatgtttcaa caacgcaaga tacttcaact gaagcaacac cttcaaacaa tgaatcagct
      601 ccacagagta cagatgcaag taataaagat gtagttaatc aagcggttaa tacaagtgcg
      661 cctagaatga gagcatttag tttagcggca gtagctgcag atgcaccggc agctggcaca
      721 gatattacga atcagttgac gaatgtgaca gttggtattg actctggtac gactgtgtat
      781 ccgcaccaag caggttatgt caaactgaat tatggttttt cagtgcctaa ttctgctgtt
      841 aaaggtgaca cattcaaaat aactgtacct aaagaattaa acttaaatgg tgtaacttca
      901 actgctaaag tgccaccaat tatggctgga gatcaagtat tggcaaatgg tgtaatcgat
      961 agtgatggta atgttattta tacatttaca gactatgtaa atactaaaga tgatgtaaaa
     1021 gcaactttga ccatgcccgc ttatattgac cctgaaaatg ttaaaaagac aggtaatgtg
     1081 acattggcta ctggcatagg tagtacaaca gcaaacaaaa cagtattagt agattatgaa
     1141 aaatatggta agttttataa cttatctatt aaaggtacaa ttgaccaaat cgataaaaca
     1201 aataatacgt atcgtcagac aatttatgtc aatccaagtg gagataacgt tattgcgccg
     1261 gttttaacag gtaatttaaa accaaatacg gatagtaatg cattaataga tcagcaaaat
     1321 acaagtatta aagtatataa agtagataat gcagctgatt tatctgaaag ttactttgtg
     1381 aatccagaaa actttgagga tgtcactaat agtgtgaata ttacattccc aaatccaaat
     1441 caatataaag tagagtttaa tacgcctgat gatcaaatta caacaccgta tatagtagtt
     1501 gttaatggtc atattgatcc gaatagcaaa ggtgatttag ctttacgttc aactttatat
     1561 gggtataact cgaatataat ttggcgctct atgtcatggg acaacgaagt agcatttaat
     1621 aacggatcag gttctggtga cggtatcgat aaaccagttg ttcctgaaca acctgatgag
     1681 cctggtgaaa ttgaaccaat tccagaggat tcagattctg acccaggttc agattctggc
     1741 agcgattcta attcagatag cggttcagat tcgggtagtg attctacatc agatagtggt
     1801 tcagattcag cgagtgattc agattcagca agtgattcag actcagcgag tgattcagat
     1861 tcagcaagcg attccgactc agcgagcgat tccgactcag acaatgactc ggattcagat
     1921 agcgattctg actcagacag tgactcagat tccgacagtg actcagattc agatagcgat
     1981 tctgactcag acagtgactc agattcagat agcgattcag attcagatag cgattcagat
     2041 tccgacagtg attccgactc agacagcgat tctgactccg acagtgattc cgactcagac
     2101 agcgattcag attccgacag tgattccgac tcagatagcg attccgactc agatagcgac
     2161 tcagattcag acagcgattc agattcagac agcgattcag attcagatag cgattcagat
     2221 tccgacagtg actcagattc cgacagtgac tcggattcag atagcgattc agattccgac
     2281 agtgactcag attccgacag tgactcagac tcagacagtg attcggattc agcgagtgat
     2341 tcggattcag atagtgattc cgactccgac agtgactcgg attcagatag cgactcagac
     2401 tcggatagcg actcggattc agatagcgat tcggactcag atagcgattc agaatcagac
     2461 agcgattcag aatcagacag cgattcagat tcagacagcg actcagacag tgactcagat
     2521 tcagatagtg actcggattc agcgagtgat tcagactcag gtagtgactc cgattcatca
     2581 agtgattccg actcagaaag tgattcaaat agcgattccg agtcaggttc taacaataat
     2641 gtagttccgc ctaattcacc taaaaatggt actaatgctt ctaataaaaa tgaggctaaa
     2701 gatagtaaag aaccattacc agatacaggt tctgaagatg aagcaaatac gtcactaatt
     2761 tggggattat tagcatcaat aggttcatta ctacttttca gaagaaaaaa agaaaataaa
     2821 gataagaaat aagtaataat gatattaaat taatcatatg attcatgaag aagccacctt
     2881 aaaaggtggc ttttttactt ggattttcca aatatattgt ttgaatataa ttaataatta
     2941 attcatcaac agttaattat tttaaaaagg tagatgttat ataatttggc ttggcgaaaa
     3001 aatagggtgt aaggtaggtt gttaattagg gaaaattaag gagaaaatac agttgaaaaa
     3061 taaattgcta gttttatcat tgggagcatt atgtgtatca caaatttggg aaagtaatcg
     3121 tgcgagtgca gtggtttctg gggagaagaa tccatatgta tctgagtcgt tgaaactgac
     3181 taataataaa aataaatcta gaacagtaga agagtataag aaaagcttgg atgatttaat
     3241 atggtccttt ccaaacttag ataatgaaag atttgataat cctgaatata aagaagctat
     3301 gaaaaaatat caacagagat ttatggctga agatgaggct ttgaagaaat tttttagtga
     3361 agagaaaaaa ataaaaaatg gaaatactga taatttagat tatctaggat tatctcatga
     3421 aagatatgaa agtgtattta atactttgaa aaaacaaagt gaggagttct taaaagaaat
     3481 tgaagatata aaaaaagata accctgaatt gaaagacttt aatgaagagg agcaattaaa
     3541 gtgcgactta gaattaaaca aattagaaaa tcagatatta atgttaggta aaacatttta
     3601 tcaaaactat agagatgatg ttgaaagttt atatagtaag ttagatttaa ttatgggata
     3661 taaagatgaa gaaagagcaa ataaaaaagc agttaacaaa aggatgttag aaaataaaaa
     3721 agaagactta gaaaccataa ttgatgaatt ttttagtgat atagataaaa caagacctaa
     3781 taatattcct gttttagaag atgaaaaaca agaagagaaa aatcataaaa atatggctca
     3841 attaaaatct gacactgaag cagcaaaaag tgatgaatca aaaagaagca agagaagtaa
     3901 aagaagttta aatactcaaa atcacaaacc tgcatctcaa gaagtttctg aacaacaaaa
     3961 agctgaatat gataaaagag cagaagaaag aaaagcgaga tttttggata atcaaaaaat
     4021 taagaaaaca cctgtagtgt cattagaata tgattttgag cataaacaac gtattgacaa
     4081 cgaaaacgac aagaaacttg tggtttctgc accaacaaag aaaccaacat caccgactac
     4141 atatactgaa acaacgacac aggtaccaat gcctacagtt gagcgtcaaa ctcagcaaca
     4201 aattatttat aatgcaccaa aacaattggc tggattaaat ggtgaaagtc atgatttcac
     4261 aacaacgcat caatcaccaa caacttcaaa tcacacgcat aataatgttg ttgaatttga
     4321 agaaacgtct gctttacctg gtagaaaatc aggatcactg gttggtataa gtcaaattga
     4381 ttcttctcat ctaactgaac gtgagaagcg tgtaattaag cgtgaacacg ttagagaagc
     4441 tcaaaagtta gttgataatt ataaagatac acatagttat aaagaccgaa taaatgcaca
     4501 acaaaaagta aatactttaa gtgaaggtca tcaaaaacgt tttaataaac aaatcaataa
     4561 agtatataat ggcaaataat taatgcatgg ctgcaaagca aataatgagt ttgtcgtaaa
     4621 aataacaaca ttttaaacta gcaataaata atatcaaagt catcatttca atgatgcaat
     4681 ctagtatagt ccacattcta aacaggtgtg gactattact tttttcactt tatattacga
     4741 aaaaattatt atgcttaact atcaatatca ataattaatt ttaagctgaa aaacaataaa
     4801 aatgttaaga caacgtttac ttcaagttaa ttattatact gaaaattctg gtatataatg
     4861 ctgttagtga atataacagg gaaattatat tggttataat attgagtcta tataaaggag
     4921 aaataacaga tgaaaaagaa attattagtt ttaactatga gcacgctatt tgctacacaa
     4981 attatgaatt caaatcacgc taaagcatca gtgacagaga gtgttgacaa aaaatttgta
     5041 gttccagaat caggaattaa taaaattatt ccagcttacg atgaatttaa gaattcgcca
     5101 aaagtaaatg ttagtaattt aactgacaat aaaaactttg tagcttctga agataaattg
     5161 aataagattg cagattcatc ggcagctagt aaaattgtag ataaaaactt tgtcgtacca
     5221 gaatcaaagt taggaaacat tgtgccagag tacaaagaaa tcaataatcg cgtgaatgta
     5281 gcaacaaaca atccagcttc acaacaagtt gataagcatt ttgttgctaa aggcccagaa
     5341 gtaaatagat ttattacgca aaacaaagta aaccaccact tcattactac gcaaacccac
     5401 tacaagaaag ttattacttc atacaaatca acacatgtac ataaacatgt aaatcatgca
     5461 aaggattcta ttaataaaca ctttattgtt aaaccatcag aatcgcctag atatacacat
     5521 ccatctcaat ctttaattat caagcatcat tttgcagttc ctggatatca cgcgcataaa
     5581 tttgttacac cagggcatgc tagcattaaa attaatcact tttgtgttgt gccacaaata
     5641 aatagtttca aggtaattcc accatatggt cacaattcac atcgtatgca tgtaccaagt
     5701 ttccaaaata acacaacagc aacacatcaa aatgctaaag taaataaagc atatgactat
     5761 aaatacttct attcttataa agtagttaaa ggtgtgaaga aatatttctc attttcacaa
     5821 tcaaatggtt ataaaattgg gaaaccatca ttaaatatca aaaatgtaaa ttatcaatat
     5881 gctgttccaa gttatagccc tacacactac gttcctgaat ttaagggtag cttaccagca
     5941 ccacgagtat aaaaattggc actaagttta cgagatatga taaataccta ttattttaaa
     6001 tatagtctac aatctatgtg gttgtaggct gtattttttg cagtttatca ataaacaccc
     6061 atcaacaaat tataccgttt ttctactttg aaagttggaa gtaacataat cttaaataaa
     6121 tatattatta attaagataa atataatact caagattatt gttaatagtt tgttcatcgc
     6181 aagttaatta ttgtttctaa aatattggta tataattttc aatggcgaag aaaacagggt
     6241 gaaaaagtcg gtttttatat caaagcaaat aagggagcat aaacaaatga aaaggaaagt
     6301 attagtacta acaatgggtg taatttgtgc aactcaatta tggcattcta atcacgcaaa
     6361 cgcattagta acagagagtg gcgcaaatga tactaagcaa tttactgaaa ttgtatcgga
     6421 agagaaagtt ataacagttg aacatgctca aattaatatt tttcaatcta atagcaattc
     6481 aaacttgatg gagttcaaca tattaacaat gggcggtaaa tcaggagcta tggttggtta
     6541 tagtgaaatt gactcatcac atttcacaga ccgtgacaaa cgcgttatta gacgtgatca
     6601 tgttaaagaa gcacaaagct tagtagagaa ctataaagat acacaaagtg ctgatgctag
     6661 gatgaaagcc aaacaaaaag ttaacacatt aagcaaaccg catcaaaact atttcaataa
     6721 acaaattgat aaggtttata atggattaca acgctaatcc aaagtaaatt ataagttata
     6781 catctcgttt ttaaatgaca atttatcccc gtaaatatta taaataatct tttcaaattc
     6841 cacatagata tagagacact aataaacctc tttgtctcga tatgatagtc tgcaacgatt
     6901 catgttgtag gctttttaat tttacaaata aggctaaata tataagttct ggcacctaaa
     6961 atatag
//
LOCUS       DAKTLH010000134         6902 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.134, whole genome shotgun sequence.
ACCESSION   DAKTLH010000134 DAKTLH010000000
VERSION     DAKTLH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.134"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..904)
                     /locus_tag="O0394_002229"
     CDS             complement(<1..904)
                     /locus_tag="O0394_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCY0500296.1"
                     /translation="MKVVGLNRMREVETELQQRFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLRRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSKVIRHAYLDVFENEPLKPNHELYELDNVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLN"
     gene            complement(910..1365)
                     /gene="bcp"
                     /locus_tag="O0394_002230"
     CDS             complement(910..1365)
                     /gene="bcp"
                     /locus_tag="O0394_002230"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCY0500297.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            1446..2735
                     /locus_tag="O0394_002231"
     CDS             1446..2735
                     /locus_tag="O0394_002231"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCY0500298.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK"
     gene            3028..4122
                     /locus_tag="O0394_002232"
     CDS             3028..4122
                     /locus_tag="O0394_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCY0500299.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(4212..4304)
                     /locus_tag="O0394_002233"
     CDS             complement(4212..4304)
                     /locus_tag="O0394_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500300.1"
                     /translation="MFDFSTILKRKFVTGWWGHNIEKAVRDFLN"
     gene            complement(4313..6049)
                     /locus_tag="O0394_002234"
     CDS             complement(4313..6049)
                     /locus_tag="O0394_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HCY0500301.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
     gene            complement(6340..6882)
                     /locus_tag="O0394_002235"
     CDS             complement(6340..6882)
                     /locus_tag="O0394_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCY0500302.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKERYKKIRQY"
BASE COUNT         2264 a         1196 c         1002 g         2440 t
ORIGIN      
        1 tattgagaaa attaactaga ttgtttttaa aaatatctaa taagtcatac tttgcttcat
       61 aatcattacc agttatatgc gctgttatag ttacattatc caattcatat aattcatgat
      121 taggtttcaa aggttcattt tcaaacacat ctaaatatgc atgtcgaata actttacttt
      181 ttaatacttc tattaagagc gcttctttaa ctatgctacc tcgtcctata tttataaaaa
      241 gtgcttcatc tttcattaat tcaaaatgtt ttttctttag taaatgaatc gtttcttgcg
      301 tttctggtaa agcatttata ataatgtcag catttggtaa tgtgctttca agtgattcga
      361 tagtatatat ctcgtcaaac tcatctttgt tctgacctga cttgctcaga ccaattaaat
      421 tcatattaaa agcctttgct aacttcgcag ttctagtagc aattgcacct gtacctaaaa
      481 ataaaactgt ttgtcctgat aggcgtttac cagttatttt cgaatcatat atatgttgtc
      541 gttggttatc atatgatagt ttcatctttt tataatcatc taaaatgaaa gctaaaatgt
      601 attcagataa ttgtttagct tgaacacctt ttccattagt taaaagtatg ccgtgatctg
      661 caatataatc taacggcaat gtatttacac ccgttgcaaa ccatgcaatc cattttaaat
      721 tcgggcaacg tcgtaaaaat gcctcattga taccgccatc ataaccaact aatatatcta
      781 aatcagccaa gtcgctctca ggtatttctg atgctttttt ataaaattta aaatctaaat
      841 ctgaaaagcg ttgttgtaat tcagtttcaa cttcacgcat acgatttaac ccaacaactt
      901 tcatatatgt caccccaaaa tgtttttaag ttcttctatt tgtgttttaa ccttaacctt
      961 ctcgataaca tctaatactt taccttgttc atctattata aaagtcgttc ttacaatgcc
     1021 catactttct ttgccaaatg atttttttaa ctgatatacg ccagtttctt tagctaattt
     1081 aaaatcttca tctactaata aatcgaaatt caatccgtgt ttctcaataa aattttggtg
     1141 ttttttcttt gaatcaccgc ttataccata tactgcaaca tctaaatcat tgaacatttc
     1201 taaattgtct ctaaagtcac aagcttctgt ggtacaagta ggtgtattat ctctaggata
     1261 aaaatatata atcgcctttt taccttttaa tgtatcattt gtaatgacag ttccgtcttg
     1321 attttctaat ttaaatattg gaaattgttc tcctttttgc aacatataat cacctttttc
     1381 ttcattataa taattttatg atacgatatt agatgaaaat attaaattaa aagaggttga
     1441 tataaatgaa ttttagtgaa agtgaacgtt tacaacaact ttcaaacgaa tatattctag
     1501 gcggtgtcaa ttccccttct cgttcttata aagctgtagg aggcggtgca cctgttgtta
     1561 tgaaagaagg acacggtgca tatttatatg atgtcgatgg caataaattt attgattacc
     1621 ttcaagcata cggtccaatt attacggggc atgcacatcc tcatattact aaagcaattc
     1681 aagaacaagc tgctaaaggt gttttatttg gtacaccgac tgaattagaa attgaattca
     1741 gcaaaaaatt acgtgatgca attccatctc ttgagaaaat tcgctttgta aattctggaa
     1801 cagaagcagt catgacaaca attcgtgttg cacgtgcata tactaaaaga aataaaatta
     1861 taaaatttgc tggatcttat catggccatt ctgatttagt attggttgca gcaggtagcg
     1921 gcccatctca gctcggttct ccagactcag ctggtgttcc agaaagcgtc gcacgtgaag
     1981 tcattactgt acctttcaat gatattaacg cctataaaga agcaattgaa ttttggggtg
     2041 atgaaattgc cgcagtatta gtagaaccaa ttgttggtaa ctttggaatg gtaatgcctc
     2101 aacctggatt tttagaagag gttaatgaaa tttcacataa caatgggacc ctagtgattt
     2161 atgatgaagt aattactgca ttccgtttcc attacggtgc cgctcaagat ttattaggtg
     2221 ttatccctga tttaactgca tttggtaaaa ttgttggcgg tggtttacca attggaggct
     2281 atggtggacg tcaagatatt atggaacaag tagcacctct aggacctgca tatcaagctg
     2341 gtacaatggc tggtaacccg ttatctatga aagcaggtat tgcattactc gaagtactag
     2401 aacaagacgg tgtttatgaa aaattagaca gcttaggcca acaactagaa gaaggtttac
     2461 ttaaattaat cgaaaaacat aatatcacag ctacaattaa tcgtatttat ggatctttaa
     2521 cattgtactt tacagatgaa aaagtcacac attatgatca agttgaacat tctgacggcg
     2581 aagcgttcgg taaatttttc aaattaatgt taaatcaagg tatcaattta gcaccttcta
     2641 agtttgaagc ttggttctta acaactgaac atacagaaga agatattaaa caaactttaa
     2701 aagctgcaga ctatgctttt agtcaaatga aataactttt aaggaagtat atttttaatt
     2761 gaattaaaat aaagcaaatg ctcacatagt agattcattt tatatcgtta actattaacg
     2821 tttaacttga tatgtcacta tgtgagtttt tattttacaa atttatacta ttttaatctc
     2881 taactaaagt tttacatcgc tatcagtatt atgtatgatt tattttaaca actataaata
     2941 agcttgaatt ttgtaactag taagtgtata acagtaatga atacgaatat tagatattca
     3001 ataaacttaa aaggggcgtc attatttttg agactaggag ctcggatttt taaaactggt
     3061 atagccatta ttttagctat gtctatcgct tctttactac cggatgatgt tggtctgaaa
     3121 gccttagctg gtgtcagtgc tgttgttgca atgcaaccta gtatttatcg ttcatttaaa
     3181 acagtttctg atcaagcatt gggtaacatt ataggtgcca tactatctgt tacaatggta
     3241 acaattttta gtgataattt tatcattatg ggtgttaccg tcatagtact tattgctatt
     3301 ttatttaaat ttaatttagc acatgttgct actttggcga gtgtaacagc attaatcatt
     3361 atgggacaac ataccggttc tttctatatt accgcctttt acaggtttgt acttgtaatg
     3421 attggtgtca taagttcatc tctagttaac tttgtgtttc taccacctaa atttgaaaca
     3481 aaaatttact ataattcatt aaatatatcc tcagatattt ttatgtggtt taaattagta
     3541 ttaaatgata cgacggagtt taataatatt aaacaagata gtcataattt aaaacaacgt
     3601 gtcgaaaagt tagagaaaat ttatgattac tatagcgaag aacgtcctat tacaaaaaaa
     3661 catattcacc aacagaatag aaagaaaata cttttcagag aagtagttca gacgactaga
     3721 caagcttatg aagtattaaa caaattgtcg cgttatcaaa acgacttata tttacttaat
     3781 aacaatttcc tattacaaat caaattagat ttagattcat taactgcttt tcacgagcaa
     3841 attttagcta gcctttctaa aaaagcacgt tataatgtga cacatgttga ttatgagtta
     3901 gataatccgc agaaaaaaga tttattgtcc acatttcagc atgagttaat aaatcatcct
     3961 tatcaaacgg aatattcgtt tgccaatgtt atgcaaattg ttgcagccat cgaagagtat
     4021 cgacaccatt tagagcattt agaccgcata agaattagct ttttcacata tcatagatct
     4081 gatgcagaca ttgaaattgt tgaagaagac tttgatttat aacattaaaa caattttatc
     4141 aattaatgcc acaatgagat aaactaatat tgaatctcga aattaaattt aaaaaagcag
     4201 taagattatt ttcaattaag aaaatctctt actgcttttt ctatgttatg accccaccat
     4261 cctgttacaa attttctctt caagattgta ctgaagtcaa acaactaata ctttataagt
     4321 tttgaatgct atataaatgc tcgtaagcac cttgttttgc aatcaattca cgatgcgtac
     4381 ctgtttcaac aatatgtcca ttttcaatta cgacaatttt gtcagcatgt gtaatagtgg
     4441 acaagcgatg cgctacgata agtgtcgttc gatctttact caacacatct aatgcttctt
     4501 gaataatgga ttcactttct aaatcaagtg cacttgttgc ttcatccaag ataagaattg
     4561 gcggattatt taaaaatatt ctagcaatcg ataatctttg tttttgacca cctgataatt
     4621 taacacctcg ttcacctact tcagtgtcat atccctgtgg caagttcata ataaagtcat
     4681 gtgcattagc cattttcgcc gcttcaacta cttcttcatc tgttgctgtt ggacgaccaa
     4741 gtaaaatatt ttccttaact gtatcagaga ataaaatatt atcctgttgc accaatccta
     4801 tttgatttct taaacttccc gttaaaaaat ctttaatgtt gtgaccatct attaaaattt
     4861 gcccagaagt tacatcgtaa aatctcggta ttaagttaat taatgttgat ttaccaccac
     4921 cactcatacc tacgaaagca actgtttctc ctttttcaat actcaaatta atatctttta
     4981 aaattggagc ttcgttatcg ttatattgaa aactaacatg atcaatatca atacgacctt
     5041 gtttaatttc aataggttga gcaccaacac catttttgat gtcataatct tcatcaatta
     5101 attggaatac acggtccatt gaagcaaaac tttgcgttaa agttgtaaat gatgcgacta
     5161 aacgacgtaa aggcccgaac aataactcta agtatccaac aaatgctgca agtgtaccta
     5221 ctgtgattga tccagaaata gcaagatatg caccaacacc gatgacaata attggtccaa
     5281 tatctgtaac tgtattaatt gcggcaaagg aataggcatt ccatcttgta tgtttcaacg
     5341 cacgtgttag gaaattagta ttctttttat caaagttttt cgcttcattg tcttcaatcg
     5401 caaaactttt aacgactgaa ataccttgaa cacgttcatg caagaatcct tgaacctcag
     5461 ctaatgcttg agatctttca cgtgtcaatt ttcttaatct tccaaagaaa acgtacaccg
     5521 ttaaaatgta aaatgggaag ataaacagtg ctgctaaagt caatttcaca tctaaaaaga
     5581 acattatgga tagtgcaata ataattgtta tacaatctaa ccaaatattc attaacccgg
     5641 ttaaaatgaa atcttttgtt tgttcaacat cattaatcac tctagatatt acttgaccta
     5701 cttgattatt agcataaaat ctcgcactta aagcttgtaa atggttgtat aactttttac
     5761 gtatatcata caatatttta ttacttgtcc attgcgccaa atattgacgt ataaattcaa
     5821 ttggtggtct cactattaca aaaataaata atgcgatacc aatggcaata gttaaatgat
     5881 gaactttttc atcagtcgtt agtgcgtggt tattaatcac gccatctatt gcatatttaa
     5941 ttaatagtgg tataagcatt ggtataccaa acttaattat cccaacaata atcgttgcaa
     6001 aaatacgata tttgtatggc ttaacaaatt gcaaatatcg tttaatcata caatcccccc
     6061 taatctattg ccctatccta ttcataagca taaaaatgaa tagaggttgg atacataatt
     6121 tgtagatgta aattcttctt acaatttaca tttttaaaat taagatatct caacctctgt
     6181 catattcatg tgattaaata cgttgtctta ttcgtatgtt atattctatt caatttaatc
     6241 tatggatact gtgtccccac acgacagcaa aagttatcat acttctttac atcactaagt
     6301 caatataaat gatttaatca gtatttacac tttatttgct taatactgtc taattttttt
     6361 gtaacgttct ttccaaactt tgataaaatc tggcgcgaat gggcccttct tctgttctat
     6421 ccattgttga agaatgtcca cgttgcgtct taaaataata tcaatatcat gcggataatt
     6481 catttgattc atatgttgct catattcatc ttcatctaat aaatgatact ttccgtttgg
     6541 atatacttta atatctaaat catagtctat atattttaat gcctcttcat cacaaacaaa
     6601 tggtgatgac aaattgcaat agtaataaat tccatcttct ctaaacatgc agataacatt
     6661 aaaccaatat tctgagtgaa agtaaacaat tgccggttca cgtgttatcc aagttcttcc
     6721 gtcactttca gtcactaacg tatgatcatt tccaccaatg acaacatgat cagtaccctt
     6781 taatattgtt gtttcagacc aaacgcgatg aatcttacca tcatgtttat aactctgaat
     6841 tttaatgttt tccccttctt taggtatgga ttctctgacc atactccaca ccaccttctg
     6901 tt
//
LOCUS       DAKTLH010000135         6789 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.136, whole genome shotgun sequence.
ACCESSION   DAKTLH010000135 DAKTLH010000000
VERSION     DAKTLH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.136"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..490
                     /locus_tag="O0394_002236"
     CDS             <1..490
                     /locus_tag="O0394_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0500303.1"
                     /translation="KNNTMSILCSELLFNYLSESEEIMMSNNSINFISSGNIRKAIEK
                     NNYDKVISFIKINDSNYRLSNVDTMKVTLYSNGSNYDKEALLINKDEFCPLRKITLDN
                     KLDSQRVMEIDSLAAIINLVKQGKGKALLPMTFENKRDIVQDISKIFEVNYYTYNHIM
                     HH"
     gene            924..1526
                     /locus_tag="O0394_002237"
     CDS             924..1526
                     /locus_tag="O0394_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500304.1"
                     /translation="MLISLVIPVFALLVIGGIIWMIIEGIVHISKKNKAIDNFFNQVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSAFLFWLVILTFGIAGIIFLMPYGLCF
                     LPFYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNITER"
     gene            1793..4945
                     /locus_tag="O0394_002238"
     CDS             1793..4945
                     /locus_tag="O0394_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HCY0500305.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNAERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSEIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFVQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQHIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDVYKNCESF
                     SKIEQVINSEKTSIEEQVYHLDNETLRDNKEIEDLDNRINYIVKQIETLNELIKSIKE
                     SNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALYRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDVKGNAVQKQFVKEHGEK
                     VVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQLPTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQMKPFYETIFKERNVK"
     gene            5002..5484
                     /locus_tag="O0394_002239"
     CDS             5002..5484
                     /locus_tag="O0394_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500306.1"
                     /translation="MEKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIEALIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKR
                     "
     gene            5689..6675
                     /locus_tag="O0394_002240"
     CDS             5689..6675
                     /locus_tag="O0394_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HCY0500307.1"
                     /translation="MKKCIKTLFLSIILVVMSGWYHSAHASDSLSKSPENWMSKLDDG
                     KHLTEINIPGSHDSGSFTLKDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKANGKARTGWLIVDYAGYTWPGYDDIVSEIIDSNK"
BASE COUNT         2605 a          818 c         1223 g         2143 t
ORIGIN      
        1 gaaaaataat actatgtcta ttttatgctc agaactgtta tttaattatt tatctgagag
       61 cgaagaaatt atgatgtcga ataactcaat taattttatt tctagtggaa atattagaaa
      121 agctatagaa aaaaataatt atgataaggt tatttcattc ataaaaatta acgactcaaa
      181 ttatagactt agtaatgttg atactatgaa agtaacgctt tacagtaatg gaagtaatta
      241 tgataaagag gctttactaa taaataaaga tgagttttgt cctttaagga aaataacttt
      301 agataataag cttgattcac aacgggtaat ggaaattgat tcattggcag caataataaa
      361 cttagttaaa caaggaaaag gaaaggcttt attacctatg acttttgaga ataaaagaga
      421 tatagtacag gacatttcta agatatttga agttaactac tatacttata atcatattat
      481 gcatcattaa ttgaaagttg attgagttta aattacagga acgattttaa aagtctagta
      541 gttagaatga gcgatgtgac agaaaagaaa ttatgccttt tattttgtaa attcgttgtt
      601 ttaaagtaaa ggtccttgta gtataggaaa aagtggatga cttctccgcc tttaccaccc
      661 tttcaagcat aatgataact gctaaatatt gattatagca ccattgcaaa actaatcatt
      721 aattgtcata ttcaactatt ttgaaaagta tcaatttcac attatcaaat tattgagtat
      781 ctcaatttat tgttatacac aaacagtatt tttgttatca tagagttaat aaatataata
      841 aattaaacaa gtaactttac aaaaattaga atgattcaat tcaatcataa ttgtttgaaa
      901 atagaggtga gcaggtgaac gatatgttaa ttagtcttgt aatcccagtt tttgctttgt
      961 tagttattgg tggtattatt tggatgatta tagaaggtat agtacatatt tcaaaaaaga
     1021 ataaagcaat tgataacttt tttaaccaag ttaataaagt aagtgagaca tataaattcg
     1081 ctactacttt tttatttcta atcttggcta cggctggtat ttctcaattt tatctatatt
     1141 atatagtatc agcgtttctt ttttggcttg tcatacttac ctttggcatt gcaggtatca
     1201 tttttttaat gccatatgga ttatgttttc taccgtttta taagcaaaaa aagaaaaaac
     1261 agacatttaa aaaatacatg gtttacacta cgattggttt gtcaatttgt ctaggcttat
     1321 ctctagtttt ggttcacact acgaaaattt atatggacga aggtggcgta agatactatt
     1381 acggtagttt tgtaatgaaa caagcgggcg gttatgctta tttagcttta gcggtacttt
     1441 caacgttgtt aattgttgcg aaaaaagcta caaataaaaa taaagaaatc gaaaccgtcg
     1501 acaatacaaa tataacggaa agataattaa gggagtgctc attcaggagt gctctttttt
     1561 gatgtccaaa tttagttgca aatgaaggca tagaagaaat ttttatagtc atttattgga
     1621 taatgaatta tggtcatatc gattaatttt attagtgagg attttacaaa catttgttga
     1681 ataaatatgg taatgataat atatgaatat ttttgaaaaa acactaatat gattaaatta
     1741 tttgttaaaa tagcattaag taaaaaacaa atagggaaca aggtgggatt tcatgagtca
     1801 attgctaaat gatacgttat cggcttggtt gttaattgaa tctttaagtc caggagaagt
     1861 aaattttaca gcggaagata tactctcagc tgaacatttt aaaaatggtg caaagcaagc
     1921 acaacttcaa agttttgatg aatattttga aatatggaat gctgaacgct ttattatatc
     1981 agaagaaaaa tcagagactg gggaacttat atttaaattt tatagacatt gcttccgcta
     2041 taatgaaatt aatttgaaaa ttcaggatat ttttgatgat tattctgaga ttcataatcc
     2101 aaatgggaca cactgttatg gttacacatt taacacagat aaacacggca aagtgatagt
     2161 tgattctata catattccga tgattatgag tgcattaaaa gaaattgaaa agaacaaaaa
     2221 tgccaatata gaagaaaaat ttaatgattc tgttgaaaaa tttgttcaaa aagtaaaaga
     2281 aattttagca gatgaaccaa ttaatgaatt taaattgaag aagatggaca aagcttatga
     2341 tgagtacttt tctgtattaa attcaaagaa agatggatta tttggacatt atgtagcaat
     2401 agaatatgtg aaagatagtg atttaccaca gccggaattt aacagcttct tcataagtga
     2461 tattgagaaa gcaagaaaat ctcccaacca aactttaatt gattacattg aaggtgtaga
     2521 agaaagtcag cacatagaag tagatgaaaa taaagaaatg tttgacaaat ttttacatcc
     2581 ttcacgtttg cctgatggac gatggccatc acagactgag tttagattgt ctttaatgca
     2641 acaacttgct gtaaaccaaa ttacgagtgg taatgaaaga ataagttcag ttaatgggcc
     2701 accaggtaca ggtaagacta ctttattaaa agatatattt gctcatctag tagttgaaag
     2761 aggtaaagag ttagctaaac taaataatcc taaagatgca tttgtcaaaa caaaaattca
     2821 tgaaacggat gataaatacg tatacttact aaaggaatct attgccaaat ataagatggt
     2881 agtcgcatct agtaataatg gagctgttga aaatatatct aaagatttac cgaaaattga
     2941 agaaattata agaaatcccg aaaaatgtaa attccctaaa tatgaacaga attatgcaaa
     3001 tttagcacat gaattaaaag attttgctga aatagctgaa gatttgattg gtgaaagtgc
     3061 ctggggctta ttttctggag tttttggtaa aagtactaat attaaccaag tattgagtca
     3121 tatgttaaaa caagatgcga atgatattgg ctttgctaaa ttactacaaa atgagaataa
     3181 tcgtatgagt tataacgagt taatgagtga atggcaatca catcaacgtg catttttaga
     3241 agagttgagg catgttgaaa tgttaaaaga agaatctatt agagcatatg atgtttataa
     3301 aaattgtgag tctttctcta agattgaaca ggttattaat agtgaaaaaa caagtattga
     3361 agaacaggta tatcatttag ataatgaaac gttacgagac aataaagaaa tagaagattt
     3421 ggataatcga attaattata ttgttaagca aatagaaact ttaaatgagt taattaaatc
     3481 catcaaagaa agcaacaaag gttttattaa caaactgaaa gcgatgttta attcagaaga
     3541 agatgaaagc tataaagatc ataataaaga gaagcaacaa ttattaacac aacagttaga
     3601 gttagagaaa tgtaaaaaaa acaaacatga agaccttgtt agcaaactaa aagaaaaaga
     3661 gaaattaatt aaacaattaa ctaaagtaca gttgcaatta gacgagttaa attcacagtt
     3721 acaagagtta gaagcatatc gtattgagtc aaaaattaca attccagaaa aagatttttg
     3781 gagtgacaac aattatgatg agcgccaagt tactaatctg tggacgagtg acgaacttca
     3841 atacagacgt gccatgctct ttttaagagc aatgatattg cataaattat tattgattgc
     3901 taataataca actatttatt atgcgattaa tgattttaaa gatagaagga aattaattga
     3961 tgcaaatcca gataaagtac acaacgcatg gaatgtgatg catttaatat ttccagtagt
     4021 tagtacgacg tttgcaagct ttaaatctat gtatgggggc ataccaaaag atttcataga
     4081 ctacttattt attgatgaag caggacaagc aatacctcaa gcagctgtgg gagcattata
     4141 tcgttcaaaa aaagttgtag ctgtaggtga tccgattcaa atagaaccgg ttgtgacttt
     4201 agaaagtcat ttaattgata acattcgtaa aaattatcat gttccggaat atctagtttc
     4261 taaagaagct tctgtgcagt ctgttgcaga caacgccaat caatatggtt tttggaaatc
     4321 tgatgctact gatagtaatc aaaaaacctg gataggcata cctttatggg tgcacagacg
     4381 atgtttaaaa cctatgttca cgatagctaa ccaaatcgct tataataata aaatggtgtt
     4441 gccaagtaat attacaaaag taggtaaaac aggttggtat gacgttaaag gaaacgcagt
     4501 tcaaaaacaa tttgtgaaag agcatggtga aaaagtagtg ggattattag ctgatgattg
     4561 gattgaagca attaaggaag gtaaaaatga accgagctca tttgtaatat cgcctttttc
     4621 agcagtacag caacagatta aacgtatgtt aaagcaacaa ctaccgacta gaattgatat
     4681 tgaacgtaca aaaattaatc aatgggtcga taaatccatt ggtactgttc atacttttca
     4741 aggtaaagag gctcagaagg tgtattttgt aataggtact gataataccc aagatggtgc
     4801 tgtgaactgg tcatgcgaaa aaccaaactt gttaaacgtt gcagtgacaa gagctaagaa
     4861 agagttttat gtaattggcg acatgcaaag aatacagatg aaaccatttt atgagacgat
     4921 ttttaaagaa agaaatgtaa aataacatac aaaaaagtat atagaggaag ttatacattt
     4981 taaaaggagc aaaattgaat aatggagaaa tttaacaact ggatattaaa tgcaataagt
     5041 ggatctcaaa cagacaagaa tgaaacaact gaagaattaa aaggggcaaa atttatcatt
     5101 ttatatgcat attcaatgct cgttttgctt gcgttagtaa tttctaacat attcattcac
     5161 attttggagc ctaaactatc aatcaccact caaatcatca tcgttttgat tttaattgaa
     5221 gcactaattg gactgcgttt cttgaaagcg tacgatgtta agcgtggcaa agataaagaa
     5281 aataagaaaa atagtaagga tttcgttaaa ctaaaatcaa ttttagtagc aattttattt
     5341 acatcattgg cgctgacagc aggtactgta gctgatatat acggtttcac tgacttagga
     5401 aatactagaa gtgatttaat cgtttggagc ataggtggta ttatatttgg cctcgtatgt
     5461 tacacaatgg aagataaaag ataacgataa ggagctggcg attataaagc tagctccttt
     5521 tttaacttat atatgtaaag aactatccta agggttttta atcatatgtc aataatttct
     5581 ataatacatt attaaaacat caattaaata agttttaaaa ttttacacat atttttattt
     5641 aaaaaagatg tataattaat gtattaaata tagaaagaag ttgatattat gaaaaagtgt
     5701 attaagactt tgtttttaag tatcatttta gtagtgatga gtggttggta tcattcagca
     5761 catgcgtcag attcgttgag taaaagtcca gaaaattgga tgagtaaact tgatgatgga
     5821 aaacatttaa ctgagattaa tataccgggt tcacatgata gtggctcatt cactttaaag
     5881 gatccagtaa aatcagtttg ggcaaagact caagataaag attaccttac ccaaatgaag
     5941 tcgggagtca ggttttttga tattagaggt agagcaagtg ctgataatat gatttcagtt
     6001 catcacggca tggtttattt gcatcatgaa ttaggaaaat ttctcgatga tgctaaatat
     6061 tacttgagtg cttatccaaa cgaaacaatt gtgatgtcta tgaaaaagga ctacgatagc
     6121 gattctaaag ttacgaagac atttgaagaa atttttagag aatattatta taataacccg
     6181 caatatcaga atctttttta cacaggaagt aatgcgaatc ctactttaaa agaaacgaaa
     6241 ggtaaaattg tcctattcaa tagaatgggg ggtacgtaca taaaaagtgg ttatggtgct
     6301 gacacgtcag gtattcaatg ggcagacaat gcgacatttg aaacgaaaat taataatggt
     6361 agcttaaatt taaaagtaca agatgagtat aaagattact atgataaaaa agttgaagct
     6421 gttaaaaatt tattggctaa agctaaaacg gatagtaaca aagacaatgt atatgtgaat
     6481 ttcttgagtg tagcgtctgg aggcagcgca tttaatagta cttataacta tgcatcacat
     6541 ataaatcctg aaattgcaaa aacgattaaa gcaaatggga aagctagaac gggttggctg
     6601 attgttgact atgcaggata tacgtggcct ggatatgatg atatcgtaag tgaaattata
     6661 gatagtaata aataaggatt caataatgat attaagacga gtatgaaaat agttagattc
     6721 taattatttt cactactcgt ttttattttg aaaataagta ataattcaac aatattataa
     6781 attgaacag
//
LOCUS       DAKTLH010000136         6720 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.137, whole genome shotgun sequence.
ACCESSION   DAKTLH010000136 DAKTLH010000000
VERSION     DAKTLH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.137"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(74..1171)
                     /locus_tag="O0394_002241"
     CDS             complement(74..1171)
                     /locus_tag="O0394_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HCY0500308.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGRRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVIIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
     gene            1543..2103
                     /locus_tag="O0394_002242"
     CDS             1543..2103
                     /locus_tag="O0394_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCY0500309.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            2156..3085
                     /locus_tag="O0394_002243"
     CDS             2156..3085
                     /locus_tag="O0394_002243"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCY0500310.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(3280..3453)
                     /locus_tag="O0394_002244"
     CDS             complement(3280..3453)
                     /locus_tag="O0394_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500311.1"
                     /translation="MNVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            3505..4884
                     /locus_tag="O0394_002245"
     CDS             3505..4884
                     /locus_tag="O0394_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCY0500312.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKIARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(5004..6032)
                     /locus_tag="O0394_002246"
     CDS             complement(5004..6032)
                     /locus_tag="O0394_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCY0500313.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            6302..6703
                     /locus_tag="O0394_002247"
     CDS             6302..6703
                     /locus_tag="O0394_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HCY0500314.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
BASE COUNT         2260 a         1156 c          995 g         2309 t
ORIGIN      
        1 tatttatacc gttttatgaa tgcgctttca aaaattatct actattctta atcctaatgt
       61 catgcgaaac tttttacttt tttatttcat ctacattgat tgtagtaact tgaatacccg
      121 cttcaacagg acttaaaaac actgtatcaa tatcttctaa atgaattgat tgagtcgaat
      181 tattgaatgt attcccaacg attaatacat ctttatgttt aacattatta taattacgta
      241 catgtatcgc atctttagac attgatcctt taaattcatt tccaattata ttcatattta
      301 tgcctgcttg ggaacctaag tcttttcctg tcatcacatc tgctgcattt ttaccatctc
      361 taactcctaa atatctaatg ccaccctcac agttaataaa cttattatta ataatgaaag
      421 catctttata cttcaaggga gttaatgcat aaccttgtat atcttcaaat atattatttc
      481 taatatgaat attctcatag tatcgattat gtctacttgc atgtgagcca atagcacgat
      541 tccaacttcc catttcgggc aattctgaag gtccaaaata acaatcttcg ataatgacat
      601 ttttcgttat cgtaccatct gtcgttccga attttggaaa agcaccaggt acttgaatgt
      661 ctaattgtat tgcttcagaa taaaagcgtt cgccactata gtctatgaat ccttcaaatg
      721 aacagctttt aatatagagt ccgttaatcc cacaagcatc aattgcatga ccactcacta
      781 cattcttaat ggtcacacct attaattgaa tatcttcagc atgcccaatg cacatagctg
      841 tattgttata aggatacgat acaccattca tatcaaactt gccgccttta atatgaatat
      901 gactgtgtcc attataacca cgataaaaac caaagcgacg accatttttt aataaaggac
      961 cagaatgaca tcggcgtaaa atagtttcat tatctaacaa aagtgttgta ttgccatata
     1021 tcgttaatgg tttgcaaata tcatacgtcc ctttcggtat ataaactgtc gttggtttac
     1081 atcttccacg atttaacgca cgttgtattg ctcgagtatc tttagttttg tttatgcctg
     1141 ttaacccata atctttcata ttgatataca taaatcgtca tcctttgtct attttctaat
     1201 tactcatata ttaaccaatt attcaaatta taatcgtgtt aggttgattt aacagatagt
     1261 cataaatctc gtgatgccat ctattagcat ttttatattc aaaacatcaa cacttgatat
     1321 tagttctaag tttgtcacat ttagtattct attacagcta ttttaaaact atcgcaaaaa
     1381 cttcttatat tatttgttga ttgcgtgatt tgctcttcca aaaaagttga ttaatctata
     1441 tacttaatct atatttatat attaaccatt agggttaaaa attactctag catttatgaa
     1501 tagatgggag tttattttat tattatatag gagagatgtt gaatgacaca tcgcgcacta
     1561 ttagttgttg actattcata tgactttatc gcagacgacg gcttactaac atgcggtaaa
     1621 cctggacaaa atattgaaga ttttattgtt tctcgtatca atgactttaa ttattatcaa
     1681 gaccatatat tctttttaat ggatttacat tatttacatg acattcatca tcctgaaagt
     1741 aaattattcc caccacacaa tatcgtagat acaagtggta gagaattata cggtaaagta
     1801 ggtaaattat acgaaacaat taaagcgcaa cctaatgtac atttcattga taaaacgcgc
     1861 tatgattcgt tctttggtac cccgcttgat agtttattga gagaaagaag tattaatcaa
     1921 gtcgaaatcg ttggtgtatg taccgatatt tgcgtgttac atacagcaat ttctgcatac
     1981 aacttaggtt ataaaatttc agtacctgct gagggagtgg cttcatttaa tcaaaaaggg
     2041 catgaatggg cacttgcaca tttcaaaaac tcattaggtg cagaggtaga acaacacgtt
     2101 taaatcgtgc taaaataatt ataaagaata caatttacaa gggagatatt tgacaatggc
     2161 taaaacatat attttcggac ataagaatcc agacactgat gcaatttcat ctgcgattat
     2221 tatggcagaa tttgaacaac ttcgaggtaa ttcaggagcc aaagcatacc gtttaggtga
     2281 tgtgagtgca gaaactcaat tcgcgttaga tacatttaat gtacctgctc cggaattatt
     2341 aacagatgat ttagatggtc aagatgttat cttagttgat cataacgaat tccaacaaag
     2401 ttctgatacg attgcctctg ctacaattaa gcatgtaatt gatcatcaca gaattgcaaa
     2461 tttcgaaact gctggtcctt tatgttatcg tgctgaacca gttggttgta cagctacaat
     2521 tttatacaaa atgtttagag aacgtggctt tgaaattaaa cctgaaattg ccggtttaat
     2581 gttatcagca attatctcag atagcttact tttcaaatca ccaacatgta cacaacaaga
     2641 tgttaaagca gctgaagaat taaaagatat tgctaaagtt gatattcaaa agtacggctt
     2701 agatatgtta aaagcaggtg cttcaacaac tgataaatca gttgaattct tattaaacat
     2761 ggatgctaaa tcatttacta tgggtgacta tgtgactcgt attgcacaag ttaatgctgt
     2821 tgaccttgac gaagtgttaa atcgtaaaga agatttagaa aaagaaatgt tagctgtaag
     2881 tgcacaagaa aaatatgact tatttgtact tgttgttact gacatcatta atagtgattc
     2941 taaaatttta gttgtaggtg ctgaaaaaga taaagttggc gaagcattca atgttcaatt
     3001 agaagatgac atggccttct tatctggtgt cgtttctcga aaaaaacaaa tcgtacctca
     3061 aatcactgaa gcattaacaa aataatacta tattactgtc taattataga catgttgtat
     3121 ttaactaaca gttcattaaa gtagaattta tttcactttc aatgaactgt tttttattta
     3181 cgtttgacta atttacaacc ctttttcaat agtagttttt attcctttag ctaccctaac
     3241 ccacagatta gtgatttcta tacaattccc cttttgtctt tacattttct taaaatattt
     3301 gcgatgttga gtataaattt ttgttttctt cctacctttt tcgttatgat taaagttata
     3361 aatattatta tgtacacgat tcatcgctct attttcaact ttcaacatat ataattcgaa
     3421 agaccattta aaattaacgg ccacaacatt caaatcaatt aatcgctttt tccaaaataa
     3481 tcatataagg aggttctttt cattatgaat atcattgagc aaaaatttta tgacagtaaa
     3541 gcttttttca atacacaaca aactaaagat attagtttta gaaaagagca attaaagaag
     3601 ttaagcaaag ctattaaatc atacgagagc gatattttag aagcactata tacagattta
     3661 ggaaaaaata aagtcgaagc ttatgctact gaaattggca taactttgaa aagtatcaaa
     3721 attgcccgta aggaacttaa aaactggact aaaacaaaaa atgtagacac acctttatat
     3781 ttatttccaa caaaaagcta tatcaaaaaa gaaccttatg gaacagtttt gatcattgca
     3841 ccatttaact atccttttca actagtgttc gaacctttaa tcggtgctat tgcagcaggt
     3901 aatacggcaa ttattaaacc atctgagttg acgcctaatg ttgcacgagt gattaaacgg
     3961 ttaatcaatg aaacatttga tgcaaattac attgaagtta ttgagggagg aattgaagaa
     4021 acgcaaacgt taattcactt accttttgac tatgtcttct ttacaggaag tgaaaatgta
     4081 ggcaaaatcg tttatcaagc tgccagcgaa aatttagttc ctgtgacatt agaaatgggc
     4141 ggtaaatctc cagtcatcgt tgatgaaaca gcgaatatta aagttgctag tgagcgcatt
     4201 tgttttggga aattcactaa tgccggccaa acatgtgttg caccagatta cattttagta
     4261 cacgaatctg taaaagatga tttaatcaca gccctatcaa aaacgttgcg tgaattttat
     4321 ggtcaaaata tacaacaaag tccagattat ggccgcattg taaaccttaa acattatcat
     4381 cgtctgactt cattacttaa cagtgcacaa atgaatattg tatttggtgg tcatagtgat
     4441 gaggatgaac gttatataga accaacattg ttagatcacg ttacaagtga ttcagcaatt
     4501 atgcaagaag aaatttttgg tcctatctta ccgattttaa cgtatcagtc attggatgaa
     4561 gcaatagcct ttattcacca aagaccaaaa cctttgagtt tatatttatt tagcgaagat
     4621 gaaaatgcta cacaacgtgt aataaacgag ctatcatttg gcggcggcgc tattaatgat
     4681 acattgatgc acctagcgaa tcctaaatta ccatttggtg gtgttggcgc ctcaggtatg
     4741 ggacgctatc atggtaaata ttcattcgac acttttacac atgaaaaaag ctacattttc
     4801 aaatctacac gattagaatc aggtgtccat ttaccaccat ataaaggtaa atttaaatac
     4861 atcaaagctt tctttaaaaa ttaatttttg agacaaaaat aaaccatgct tccataattt
     4921 gatgttcttg aaaacaataa atttcaaggt atacacaaat tttttggaaa catggttttc
     4981 tttttatcat gattaattaa agattaaaat atgacacata caccttcaga tgctacacga
     5041 gtgttatcac atagcgtaat tttacctgtt tctttatttc tttcgaaaac agttacaact
     5101 gaatctcctt gctcatgagc acaaactaaa aggtcatctg atgaggcaat attaaaatct
     5161 cttgggaatt gaccaccact ttcagtaatt gttactagtt ctaagtgttg accattatca
     5221 agaactttaa aaattgcaat gctatcatgc cctctattag atacatataa gaattgttga
     5281 tcatgagata aacgcactgc tgcaagttta gtatctccat caaagttttc aggaattgtt
     5341 aaatgacgct cgagctcttc aaatttaccg tcattatatt ctgcaacact cacagtattt
     5401 gataattcgt gtacgacata agcaaatttt ccattatcat gaaattcaat atgtcttgtc
     5461 ccatcactat ctttaaataa agattcttta taaaattcaa acccgttgtc atcaaattta
     5521 taagtaacga tacgatcagc acctaaatct gttactgcaa cgtacttacc atctggagtt
     5581 tgattaatat aatgtgcgtg tggatgatct tgtctttcat gtgtacctgt tggaaaatca
     5641 tgtgcaagtt cttgaattag acgtataatt tcacctgtgt gcgtatttaa ttcatacata
     5701 cgtatgatgc cagcaccata taccgcttca aataaatatc gtttatcttc tgaaatcgat
     5761 acataacaac ctgtaccagc ttttgaagac aaacatttat ttaataaatg caattcacca
     5821 ttgtcatcaa ttttcaaact tgcaacaccg cattgttctc cttctttgtt gattccatat
     5881 aaaacttcat tattacgcac caaatatgta gacgcttcta attcaaatcc tgtttctaat
     5941 aaatcaatac gtgactgatt ttcgtttaat tcaaaacgat aaatcccttt accattcttt
     6001 ttagtgtaag aaccaatata tccatttgtc attactttta cccctccatg ttagtcttat
     6061 tttcatttta ttaaatttta gctagaatta acaatcattt atcatttcct taaagctaaa
     6121 actatttaac tactttaaaa gaactatttg ctgtgatatg tcattattcg attttaatta
     6181 atccctcttt tttacttaca cgagcattaa ttattaacgc gtttatgctt ccacaaaatt
     6241 tttgttgcaa aatagaacac atgttcgtat aatgtaatta tcagattggg gtgaattaag
     6301 gatgacaagt tatgaagaag aaactgatta tcgtaacgtt ccaagagaat tactagataa
     6361 caatatacct atgggaagag gcatgattaa atgggctcct tttgcaacat tacccgaaca
     6421 attcgaaacg attcaacaat acattatcga ccaaaacaaa attacacgtc ctgtattatc
     6481 agatgatcaa ttggctgagc taaacattcg gctacacgag gcgctacaat atgcccaacc
     6541 tgtagaagtt aaattttata ataatggctt tgtagattca gtacgcttaa ccatttatcg
     6601 tattgatgct attaattatg aaattgatgg ttatgtttac aatcagcaac aacgtcaaaa
     6661 aatttctatc tttgatatct tagatattgt cttgttacct taatgctttg aacataatta
//
LOCUS       DAKTLH010000137         6707 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.138, whole genome shotgun sequence.
ACCESSION   DAKTLH010000137 DAKTLH010000000
VERSION     DAKTLH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6707
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.138"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            87..452
                     /locus_tag="O0394_002248"
     CDS             87..452
                     /locus_tag="O0394_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HCY0500315.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
     gene            634..1911
                     /locus_tag="O0394_002249"
     CDS             634..1911
                     /locus_tag="O0394_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCY0500316.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
     gene            complement(2315..2671)
                     /locus_tag="O0394_002250"
     CDS             complement(2315..2671)
                     /locus_tag="O0394_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCY0500317.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     TTIEKMISEKDESIEKNVKLPSDSDVKVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
     gene            complement(2936..3358)
                     /locus_tag="O0394_002251"
     CDS             complement(2936..3358)
                     /locus_tag="O0394_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HCY0500318.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEIVTEQEVIDWLWETQD
                     KSFFSSKEDPELCVLRVVPQSIKLMNDKSLDTPIKIDL"
     gene            complement(3429..5534)
                     /gene="rsp"
                     /locus_tag="O0394_002252"
     CDS             complement(3429..5534)
                     /gene="rsp"
                     /locus_tag="O0394_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HCY0500319.1"
                     /translation="MTCQLKIHHTLSTIPSRNINNIMVLFSLTSKLNITINGETKDVS
                     NYIILINHGDIYNINHGENIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIA
                     DLISTPTSSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNI
                     DAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTV
                     SELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTID
                     LTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPV
                     VFIDDIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQ
                     DYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQV
                     YDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVY
                     NNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSP
                     LPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNE
                     LPQDSLMIIKTLNHEHGSIQHLLPISDQLVYIEKEILDELDKTNYPKTELAVQEETGR
                     TFELKLNHDEVKYICFKPS"
BASE COUNT         2373 a          970 c         1037 g         2327 t
ORIGIN      
        1 aaaaatttgt tacaatctgg taaaaactct ttattaaata aattgattcg ttcatactga
       61 atagataagc aaaaaggagt tacataatga agaagaaaaa aaacgcgatt aaagttatca
      121 ttatattatt tatttcatta attgttgcag tagcattttt ctttggtctg aaaacatatc
      181 aaggccataa gaatattcaa ctaattgaca gttacttgga agagaaaaat ctaaaagata
      241 aaattaaatc agaaaaaaca gagtatagtg ctaaaaaagg tctcttctat aaggaagtta
      301 catttaaaga tgaacctggt gtaacttatg ttgtgcaacc aatcagtact aataaaggat
      361 tatttgttga aggctttgat acagagacta agaaaagttt gaaaactgca aagcataaat
      421 atttcaatca aaattataaa ccatcaaaat agtgaaacgt taaactggtg aagcagttta
      481 gtgccttcaa ttttaattat ttgaggtaaa cagtttttgt atggaaatta gtgatttttg
      541 acatctataa aagataaata ttttaatttt ttatagataa ttatcaattt tctgatataa
      601 ttgagtgatg tcagatgaag gggagtttgt actatggctc tattcaagag aaaaattagc
      661 ctacctatgc aagttgttat tgcattagtg ttaggtgttg tcgtaggact tttattatat
      721 ggacaagaaa acgtagcaaa ttacattaaa ccatttggtg acgtattttt aaatttaatt
      781 aaaatgatcg ttataccagt tgtattttgc tcactagcgc tttctatttc gaacgttggg
      841 gaatcgaaaa ctgtagggcg ttatggctgg aaaacaattt tatactttga aattattaca
      901 acaatcgcaa taggtttagg gattatcttc ggtaacctat ttaaaccagg tgctggatta
      961 gacccaacaa aattacctaa aggtgatatt tctaaatatc aatcaactgc acatgcagca
     1021 gaacaatcta catatggaaa tcattttatt gataccattg tacatattat tccgacaaac
     1081 ttttttgaag ctttaaataa gggtgaatta ttacctatta tcttcttcgc agtattcttt
     1141 ggattaggat tagctgctgt aggtaaaaaa gcagaaccag ttaaagaatt tttaagcgga
     1201 tcgcttgaag ctgtgttctg gatgattaat aaaattttaa aattagcacc acttggagtg
     1261 tttgcattca tttgtactac aattattaca tttggtgcat ccgcattatt accactatta
     1321 aaattagtat tagttgttgt ctttgcaatg gtgttctttg tattcgctat actaggacta
     1381 gttgcatgga tgtgtggtat taatatcatg aatattatta gaatcttgaa aagtgaattg
     1441 cttttagcat tttctacatc aagttcggaa gctgtacttc ctgtaatgat gaagaaaatg
     1501 gaaaacttcg gttctccaaa agaaattact tcttttgtta taccaattgg ttatacgttt
     1561 aacttagatg gatcagcact ttatcaatct attgcagcat tattcgttgc acagatgtat
     1621 ggaatgcact taacattatc agagcaaatt gtgttgatgt taacattaat gattacgtct
     1681 aaaggtatgg cggcagtacc aggaacttca atcgttgttt tattaacaac attaggtgcc
     1741 atgggcttac cggcacaagg tttagcatta attattggtg ttgaccgtat cttagatatg
     1801 gtacgtacat gtgtaaacgt tattggtaat gcattatcaa caatcgttat agctaaatgg
     1861 gaaaacgtat atgacaaagc aaaaggtcaa gaatatttaa aatcaattta aaaaatacta
     1921 tctgacattt aaagcccctt acaacctttg gttgttaggg gcttttatgc atgcgctaaa
     1981 gcgcaggcga acggaagcgt agtgacctca ggttgttagg gacttttatg catgcgctaa
     2041 agcgcaggcg aaccgaagcg tagtgacctc aggttgttag gggcttttat gcatgcgcta
     2101 aagcgcaggc gaaccggagc gcagtgacct caggttgtta ggggctttta tgcatgcgca
     2161 aagcgcaggc gaacggcaag tggagctatt caatatgaaa gtatttcaaa tataaaaagc
     2221 agcagtaaaa tgattccaaa ttggaaatca acttaccgct gcttttactc gtatatacct
     2281 tgatggcaac taaaatttgt aatttaaatt acaattagtg tttcatgtgc attttgccca
     2341 tgtcatgaat tttcatcatt aaaccatgtg gtacatcgtc atggtcaact tttttaactt
     2401 taacaatttt atctgaatct gctaatttat aaacaattac aaaatcacct tttttaactt
     2461 taacatctga atctgaaggt aatttaacat ttttttcaat actttcatct ttttcactaa
     2521 tcattttttc aatagtagta ttatctttca taattccata gtatgtatct ttctttccgc
     2581 cagtcatcat catgattact aatacaatac atacaattaa catgatggcg attaaactaa
     2641 taccaagacc aaaacctttt ttctttttca tggttcatca atctccttaa atgtagttaa
     2701 ttctgttata cctaaaagtg caacagtttg caaatgcagt atttatatta cataaaatta
     2761 atttaaaaag acacacccta atcacgttaa atgtgaataa agtgtgtcta aaaaacaata
     2821 cattgcaaat tgaattgtaa acatattaaa cactttaatt atagcgagtt attttaagta
     2881 tttaacattg ttatatctag aattcataaa agttatttcc tatatacact ttgtgttata
     2941 aatcgatttt gataggtgta tctaatgatt tatcattcat tagcttaatg gattgcggaa
     3001 ctactcttaa aacacataac tctggatctt cttttgaact gaaaaagctt ttgtcttgtg
     3061 tttcccatag ccaatcaatc acttcttgtt ctgtaacgat ttcgattgtc gcttccatct
     3121 caacgaaact acgattagtt gtgtcgttat agcctaacaa aacatatgct gctggattat
     3181 ctttaatttc tttgacctta gcagaatgga tattcgtttt agtatataaa gtaagaccat
     3241 cattataaaa gaccatatat ctactattag gtttattatt atatgcagtt gataatacac
     3301 caacttttga cgttgctaac acgttttcaa ttgcttgaat tgcttgtgag ttactcatta
     3361 caaatcatct ccttatgata taaatagcct ttaacgtaag aaactaaaca caagaggact
     3421 gttagtattt agcttggttt aaagcaaata tatttaacct cgtcgtgatt taacttgagt
     3481 tcaaatgtac gaccagtttc ttcttgaact gcaagctccg ttttagggta attcgttttg
     3541 tctaattcat ctaaaatttc tttttctata taaacaagtt gatcgcttat tggaagcaaa
     3601 tgttgaattg aaccatgttc atgattcaat gttttaataa tcatcaaaga gtcttgaggt
     3661 aattcattat ggaaaatgaa atcttgtttt acgtctgaca tatatcgatc attaatttta
     3721 ttgaataata agaaatggta gttattatct ttacgactca atacataatt atttgtaatt
     3781 tcaatatctt cattaacaaa aggtctaaac attttatata aatgcattaa tggtaggggg
     3841 ctaccatatt tgttgtacaa ggaaatataa tttgaatggt catctaataa ttgatagcta
     3901 atgccaccgc catatcgtaa taatgcgatt aagtgataca ctatgtcaga taattgaatt
     3961 ggattcttac catcattagt atatttaaat ccgcttacag acaggttatt atatacaaat
     4021 tgtgttgatg atatacctga atctaaaata aatttcttat actgttcaaa atgagtgtca
     4081 gtttgatgat agtgttggtt tttagtgata agatgtgttg cagtttttga attttcaata
     4141 tcaataaagt aataatggaa tttaagacgc tgcatgacat cataaacttg ttccacggtt
     4201 gaataagttt ctaaaaagcc atcgactgtc acactatatt taatttcttt atttttgttt
     4261 ttaattttaa gatgacataa atgaatttct tctgctgtca tagaattaga gcaaaatacg
     4321 agcataaatt taactaattt cttgcttgtc ttatagtctt gattgccttg taaaaatgtt
     4381 agtattaatt gcttcatcgt ttcgaattgt tgtgttgatt taatagcgat tgctaaacct
     4441 atattttttt cgaaaagctt ttcaaaacat ctatttaaca ggttgtaatt cgtttggcta
     4501 atctcaatat cgtgaatatc gtcaatgaaa acgacaggtt ttggtaagtg ctcaaagtta
     4561 atatcgtaat attcattgaa aataaattga aatagttcat tgaaattact aaatcgaaca
     4621 aatactttgg cacgctgact tcgatctttc ggattaaaat catctatatg aatatctgtt
     4681 gtatttgtag ccagttgagc tgttaaatca attgttgaaa ttaactcagt gaattgtgat
     4741 acgttatctt gttggaagtg tatttgtggt ggttcagtaa tcttggaaac gagtgaacgg
     4801 aactgtttag gactaaaatg caagtaattc ttaaattgat ttgcaaaatt tgtatgactg
     4861 ttaaaaccgg ccaattcaga aacagttgta atagaatgtt ttgtagaaag taataaattg
     4921 atggcgttaa caagtttaat acttgtaaaa taatctttaa aattcatact caaatatcga
     4981 acaaaaagat tagaacaata tgattcagag atgttgcaat gcatcgctat atcttttaaa
     5041 gacaagtgcg catcaatatt gtcatgaata tagtttacac agtctatgaa caccggattg
     5101 cttaaagcaa tatttggtaa atattcatga tctattctta taaaagcttc tttaagtaaa
     5161 gtgtctataa tactttgacc tatatttttc cccattaaag aagaacttgt tggtgtactg
     5221 attaaatcgg caattaatga cttaatataa ttacttgatt gtaataaatg acgatctaaa
     5281 taaccattaa agaaatcatc atcttgttga taaaaataaa aaacgggtat cattaattcg
     5341 attatatttt cgccgtggtt aatattataa atatcaccat ggttaattaa gataatataa
     5401 ttacttacgt ctttggtttc accattaatc gtaatattga gtttacttgt aagtgaaaat
     5461 agcaccataa tattattaat attacgactt ggtattgtcg ataatgtatg atgtatttta
     5521 agttggcatg tcatatttct ctctcctgcc taatatatat tgaaatctat catttaaatg
     5581 taaaacatgg aaatttttaa aatcatggat agtataccta ttatagttga atatttttga
     5641 aaaggtaata gtgaaaaata aaagaaatta atgaaaactc ctaggaaaac gcttgttttt
     5701 atgcattgcg aacaatataa aagtaaaatt agaaagatag taagcaaaat ttggaaattt
     5761 tcgatagagt ctaattaaat tatttggtgc ataattaata tagatttcaa acctatgtat
     5821 ttcaaaaaac aatacatagc cattttgaac taacttgcta atcttttatt gatattcaac
     5881 tatgttataa aaaaagcaaa ttgaccaaaa tggaagtttg aatcaaaaaa cattaaatgt
     5941 acccatgact taaattttat cgccattatt taagtttgga catacacttt tgtcatcaaa
     6001 cgctgaaaat tagtacgatt gaaatcactt cttgttataa aaattcttag tagccctgtt
     6061 acaaaatatt cgctacaaat tattcaaatc aacaacaata accatcaact aagaaatttt
     6121 aaacaatcta ctaaaaaata aagcgtttaa aagttctgtt tagaaaacat caagataagt
     6181 ataaacatct agaaaagtga acatctaaga aaatccatct agttattgct gtaacaatta
     6241 gaataatcat ttgaaaaagt gcattagaca acgaaattca ccatcaagat aatgaagcaa
     6301 caagaaacag cacacatcaa tgaattaaca ccttagattc atcatgagaa aatgcatggc
     6361 acaactagat ttaccatcaa gataattctt aagcagcgaa gaattaacat ctagaaatgc
     6421 ataaacatct tagattttac atcaagagat gcgataacta taaacaacta gaaacgtcac
     6481 caagatagtg caacaacaaa gaatcagcat cttgaaatcg cactaacatc taagaatcaa
     6541 catcaagtat tatattaaca tcatagataa cttcatcaag acaggcatcc acatctaaga
     6601 aactcatcta gaatagctaa cacctaacat caaagaatat tcatcaagaa cagtatctaa
     6661 cagaactttt tgactatata tgaattaatt atgttactag ctaaatc
//
LOCUS       DAKTLH010000138         6637 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.139, whole genome shotgun sequence.
ACCESSION   DAKTLH010000138 DAKTLH010000000
VERSION     DAKTLH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.139"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            19..654
                     /locus_tag="O0394_002253"
     CDS             19..654
                     /locus_tag="O0394_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0500320.1"
                     /translation="MKLNNYSLKVKNKQLVDNCDLNFYLGQINHIVGKNGVGKSLLAK
                     DFLLNNSGNIPKSISQNVTLISSSSNIPNDITKDFLLSLLKSKFENNRQTFDKIYNIL
                     NIEAIPSNVLLKNLSDGQKQKLKLLSFLLEDHDLIILDEVTNALDKKTVNEIYEFLND
                     FIQSHQTKTIINITHNLSDLSALPGKYFIFKDLQIEEYQSKEEVINDYINL"
     gene            660..1391
                     /locus_tag="O0394_002254"
     CDS             660..1391
                     /locus_tag="O0394_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HCY0500321.1"
                     /translation="MSTLKLEFKKSISNKIIYTLVVLFTFLFLLGYFLPIGIDKVKSL
                     SYSQFFFSSYTVATQLGFLLFSFVIAYFINKEYSNKNILFYKLIGDNIFTFFYKKVAV
                     LFFECLVFIILSITIISIIYSDFSHYLLLIILFSLVILQYILVVGTISMVSPNILISL
                     GISIVYWIGSVILVAINKNIFGIVAPFEASNTMYRAVEKILNNESTFMCPTEIINIVS
                     FFVLLFIVNTIVLLLSRKRWLKIGM"
     gene            complement(1551..2171)
                     /gene="srtA"
                     /locus_tag="O0394_002255"
     CDS             complement(1551..2171)
                     /gene="srtA"
                     /locus_tag="O0394_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HCY0500322.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVGVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            2365..2856
                     /locus_tag="O0394_002256"
     CDS             2365..2856
                     /locus_tag="O0394_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0500323.1"
                     /translation="MIRYAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERVAWFET
                     KQRKHEPIFVFEENGSVLGFATFGSFRPWPAYLYTIEHSIYVDASARGKGIASQLLHH
                     LIVEAKAKGYRALVAGIDASNKASIQLHQKFAFKHAGTLTNVGFKFNRWLDLAFYELD
                     LQD"
     gene            complement(3294..3464)
                     /locus_tag="O0394_002257"
     CDS             complement(3294..3464)
                     /locus_tag="O0394_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500324.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
     gene            complement(3526..3624)
                     /locus_tag="O0394_002258"
     CDS             complement(3526..3624)
                     /locus_tag="O0394_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500325.1"
                     /translation="MIKKKHLSGSLVPDRCFLIISEEVERFMENAY"
     gene            complement(3643..4542)
                     /gene="sdaAA"
                     /locus_tag="O0394_002259"
     CDS             complement(3643..4542)
                     /gene="sdaAA"
                     /locus_tag="O0394_002259"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCY0500326.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            complement(4556..5236)
                     /gene="sdaAB"
                     /locus_tag="O0394_002260"
     CDS             complement(4556..5236)
                     /gene="sdaAB"
                     /locus_tag="O0394_002260"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCY0500327.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            complement(5239..6282)
                     /locus_tag="O0394_002261"
     CDS             complement(5239..6282)
                     /locus_tag="O0394_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCY0500328.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
BASE COUNT         2105 a         1222 c          972 g         2338 t
ORIGIN      
        1 tacataggag ttgtatacat gaaactaaat aactattctt taaaagttaa aaacaaacaa
       61 ctagttgaca attgtgattt aaatttctat cttggtcaga tcaatcacat tgttggtaaa
      121 aatggtgtag gaaaatcttt attagctaaa gatttcttac taaataatag tggaaatatc
      181 cctaagtcca tttctcaaaa tgtaacctta atatcaagtt catcaaatat tcctaatgat
      241 ataacaaaag attttttatt atcattgtta aaatcaaaat ttgaaaacaa tcgacaaaca
      301 ttcgataaga tttataacat actaaacatc gaagcaatac cgtctaacgt actactaaaa
      361 aacttgagtg atggtcaaaa acaaaagctt aaattattaa gtttcttatt ggaagatcat
      421 gatttaatca tattagatga agttacaaac gctttagaca agaaaacagt taatgaaatt
      481 tatgaatttt taaatgattt tattcaaagc catcaaacta aaactattat caatattaca
      541 cataatttat ccgatttaag tgctttgccg ggtaaatact ttatttttaa agaccttcaa
      601 atagaagagt accaatcaaa agaagaagtc ataaatgatt acattaattt ataattgagg
      661 tgtccacttt gaaattagaa tttaaaaaaa gtatttccaa taaaattatt tacacgcttg
      721 ttgtgctatt cacattcctt ttcttacttg ggtatttttt accaataggt atagacaaag
      781 ttaaaagttt aagctatagc cagtttttct tcagttcata tactgttgca acgcaattag
      841 ggttcttact attttcattt gttattgcat attttattaa taaagagtat tcgaataaaa
      901 atatcctgtt ttataaattg attggcgata atatctttac cttcttctat aaaaaggtag
      961 cagtgttatt ttttgaatgt ttagtgttca taattctaag tatcactatt atttcaatta
     1021 tatattcgga tttctcacat tacttactat taataatctt attttcactc gttatcttac
     1081 aatatatctt agttgttgga acgattagta tggtttcacc taacatttta atcagcttag
     1141 gtatcagcat tgtatattgg attggttcag tcatcttagt tgccattaat aaaaatatat
     1201 ttggtatcgt tgctccattt gaggcgagca atacgatgta tcgtgccgta gaaaaaatat
     1261 tgaataacga gtcaacattt atgtgtccta ctgaaattat aaatatagta tcattcttcg
     1321 ttcttctatt tatagttaat actattgtct tattactaag tagaaagaga tggttgaaaa
     1381 tcggtatgta gtataaaaaa ggagcggtat acattgccgc ttctttttat tatgtatttt
     1441 tacaatctcc accaatgaca cgtcgtcatt gctacctcat accaaacaaa aaacagtctc
     1501 gcaatcaaga ctgttttcca ctcaatatat tcatccatta gcgtaataga ttatttgact
     1561 tctgtagcta caaagatttt acgtttttcc caaacgcctg tcttttcatt gtaatcatca
     1621 caagtaatta atgttaattg tttatcttta cctttttgtt catctagaac tcctacatct
     1681 gtaggcttaa catctcttat acttgtcatt ttatacttac gtgtttcatt accaacttta
     1741 aagtacacca tactaccttt tttggctgct ttaagatttg taaattgata gttcggacgg
     1801 tcaatgaaag tgtgtcctgc aattgaaata ttttgatcat ctagtgattc attttcttct
     1861 gcaaagctta cacctctatt taattgttca ggtgttgctg gtcctggata tactggttct
     1921 ttaatatcag catctggaat ttcaatatag cctgccactt tcgatttatc tttcggaatt
     1981 tgaggtttag cttgctgctt tttatcttta ctcgcctgtt cttttacatt tttatcatat
     2041 tgttcaatct tttcatcttt atctttatcg tgaagataat tatcgatatg tggtttagca
     2101 aacaaatatg ctgccactag gataagtacc acaccagcga ttgtcattaa tcgatttgtc
     2161 cattttttca tacgttaagg ctccttttat acatttcaca atgcctgtta acatttcata
     2221 tagtatacca ttaatttcaa aatgactcat agcaattcat tttatactat aaaatttaca
     2281 tgtatacttt tacgttagat ttcattacac atatttgcat tcaaataacg aaacgctttt
     2341 aataattact aagggggaat tgatatgatt agatacgcta aaaaagagga tttaaacgct
     2401 atattagcga tatacaatga tgccattatc aatactacag ctgtttatac ttatgaacca
     2461 caaaccatag acgaacgtgt cgcatggttt gaaacgaaac aacgtaagca tgagcctatc
     2521 tttgtatttg aggaaaatgg aagtgtctta gggtttgcga cgttcggttc atttagacct
     2581 tggccagcat acctatatac aatcgaacat tctatttatg tcgatgcttc agctagagga
     2641 aaaggtattg ctagtcaatt actacaccat ttaattgtgg aagcaaaagc taaaggttat
     2701 cgtgcgctag ttgcaggcat tgatgcttcc aacaaagcga gtattcagtt gcatcaaaag
     2761 tttgctttta agcatgccgg cacactgacc aatgtaggtt ttaaatttaa tagatggtta
     2821 gatttagcat tttacgaatt agatttacaa gactagtaat gtttgaatca cataatataa
     2881 acaagacaac catgttaatt cccttaacat aacaagccaa catataaaat tttaaacttc
     2941 tcaggggagt gggacagaaa tgataaagag ccactaatga tttattatgt agtggttctt
     3001 acacattagc cacagctaat gtgtacttaa aaataggaat acatgagtaa aactcatgca
     3061 taagaaatac taatttctat agaaaaagta tttctttatc gtcgtcccac cccaactcgc
     3121 acattattgt aagctgactt ttcgtcagct tctgtgttgg ggcccaaaaa gcttgttaca
     3181 agcgcatttt cgttcagtca actactgcca atataacttt gtagagcata ttacattgat
     3241 ttacattgtc ccttttattt attcttttca aatactatcc ccatagcttt gatttaacgc
     3301 tttttctcaa taacaaaacg aatatagtag aacatgaaaa cgataatcat gctgagcgat
     3361 aaagatttaa ataatagatt gacccacgtt ccctcagtcg tatatccata tgtaatcgtt
     3421 gtgttaatga tgaatgctat aaagatgatt gatagtctta gcatatcatc actcctttta
     3481 agttatttta gatatacggg ggcgcttttg caatcactat tttgattagt atgcattttc
     3541 cataaatctt tcaacttctt cagagataat taagaagcat ctatctggta ctaatgatcc
     3601 agacagatgc ttctttttta tcaatatttt attgttatct cattaattat ttttaaccat
     3661 atcttcagct gtgccaaaga ttttacgttt aattgcttcg ccagttggtg tgcctgctag
     3721 tccacccaat ccagtttcac gtaatgatgc aggaaggtta cgaccaacct tatccattgc
     3781 ttcaataact tcatcaacag ggattctact ttcaatacct gctaatgcta aatctgctga
     3841 aattaaagcg ttacccgaac caattgcatt tctcataaca caaggaattt caacaagtcc
     3901 ggctactgga tcacaaacta aacctaataa attacttatc gctaatgcca tagcgtgccc
     3961 ggatgcttct ggtgatcctc cgaatatagc tactgctgca gctgcggcca ttgcagatgc
     4021 tgaaccaact tcagcttggc agccacctgt tgcaccagct acacttgcat tgtttgctac
     4081 gacacgccca aacaatgctg aagtgaataa gaaatcaatc atttgctctt ctgttaaatc
     4141 atgtgttttt tctaatttaa aaagtgcacc gggaatggta cccgaggaac cagctgttgg
     4201 cgttgcacaa ataataccca tcgcagcatt gacttcattt gttgcaatgg cacctttgac
     4261 tgcgtcaatc atttcatatc cagacaaagc atgatgtgtt tcattataat cacgtagttt
     4321 agcagcatca tgaccagtgt agcccgttac actttcaacc ccatcacctg tcgtcccttt
     4381 gattactgcg tctcgcatga catctaaatt ttgtttcatt tgcgctcgca cttcatcacg
     4441 tgatttaccg cttaattcca tttcttcttt aaccatgata tccgcaaatg acatattatt
     4501 ttctacggca taatctatag tctctctaat tgaatcaaac atgtttattc cccctctaat
     4561 ttatatagga aacgtttacg tcactgtatt tctctttaat tgtatttaat gttgattctg
     4621 agattgcttt atttaatggt attacaacca agcatttatc ttcatctatc ttaataaatt
     4681 catctttaca gtctaatttc atatcgttga tatcattgat gaaatgattt acttgtgctt
     4741 tagtcatatt tccgtcaaca actaaaattg gtaatccatg atttaaatct acttctagtc
     4801 catttatatg aataccttta attttaattg taccaccacc gattgaaata ccgataattt
     4861 caatgtagcg accatcatta cgagatgatt tgatataagc acagtttgga tgttgaccaa
     4921 tactatcgcc ttcttcttcg atgatatcta ttttaatacc atcatcagct gcaatttcta
     4981 atgaagattt aattcggtta tcaaatgttg aatatcccat tgctccaccc acaatagcga
     5041 catctgtacc atgtccttgg tgtgtttgag caaatgattc ataataatgt atttcaatat
     5101 ttttaggaat atctcccaat attgcgcgtg ctgaattccc aatctttact gcaccagccg
     5161 tatgagaact tgaagggccc atcataactg gtccgataat atcgaaagca ctttgataat
     5221 catagctctt tgccataatt aaacactctc cttaatatga ttctttttgc tcggcatttt
     5281 aaagttgata ttcattaaat taaacttatt aattagtgtt ccaataatat aggctaagac
     5341 aatgctgaca ataatcactg ttgcgattgt tacaattgat gtcactgcat tattaaagcc
     5401 aaacaatacg atggcgcctg caattggtgt tgccatacct ttgacaccta ttactagtcc
     5461 gctaaatgtc acgatacatg cgttgacgac cccaatcagt gcatttgtac catatagttg
     5521 tactggatat tgcgctatta aatcaatttg cgtcaatggc tcaatacaaa ctgcaaatgc
     5581 ttttgacggt ccaccaatgt ttaattttcg gaataaaata aggttaacaa atgagctacc
     5641 tgtacatgtt agtgctccaa tagccatagg aacacctgtc agtcctaata aacttgttaa
     5701 taccattgaa cttagcggtg tcatacctgt aacaggaatc actagtccta aaatgaccgc
     5761 taatgcatat ggattgttat cacctaccgc agtgacagca ctacctattt gttttaatgt
     5821 tgctagcaca ccaggtgtaa tgattgatgc aagtccgaaa gcaattgctg gtgcaaataa
     5881 gatcaccaca attaagtcca agccttctgg aactttcttt tcaatccatt taattaaaaa
     5941 agctacgcca taagctgcga tgaatgctgg taataattta aagtcatgta atactaaacc
     6001 aacaatgacc gcaaatactg gtgcaacgcc taagtttaag cacgttagaa tacctactgc
     6061 gataccgctt aaacttcctg ctaaatcccc aatatcttgt agaaatttaa tatcaaatac
     6121 gccaccaata gcataactta agaatgcttg tggtagaaat gtcgcacaag ctgcaccgga
     6181 taatgcttgt agtccttgtt taccgtacgg tgcatacttt aaaaatagcg tcatgatcac
     6241 taaaaccaag actaatgtgc ctacacctaa cagaatatcc atttcccaaa aacctctctc
     6301 tatgtttatt ttattttcag accataaaca tcgtacaccc ttaagaaaac gttttcaact
     6361 tttatctgtt atcaaatcaa atatttaagt gaaatatttg atatttgtga aagattttta
     6421 aaatggattg tttcaaaaac tacttatgtt gtcgttaata tttactaatt aactttttac
     6481 tctatatttc aaacagttgt gtgacagttt tttgataact tttttacatc tgaaagtaag
     6541 taatatttct aaaaactttt aatatttata cactttatct ttcgagctat ttattttttg
     6601 tttttgtgct gtgtttataa aaccgcacac tatattc
//
LOCUS       DAKTLH010000139         6614 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.141, whole genome shotgun sequence.
ACCESSION   DAKTLH010000139 DAKTLH010000000
VERSION     DAKTLH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.141"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            228..626
                     /gene="nrdI"
                     /locus_tag="O0394_002262"
     CDS             228..626
                     /gene="nrdI"
                     /locus_tag="O0394_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCY0500329.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            589..2694
                     /gene="nrdE"
                     /locus_tag="O0394_002263"
     CDS             589..2694
                     /gene="nrdE"
                     /locus_tag="O0394_002263"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCY0500330.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            2812..3783
                     /gene="nrdF"
                     /locus_tag="O0394_002264"
     CDS             2812..3783
                     /gene="nrdF"
                     /locus_tag="O0394_002264"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCY0500331.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            4297..4452
                     /locus_tag="O0394_002265"
     CDS             4297..4452
                     /locus_tag="O0394_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500332.1"
                     /translation="MFYFTLSKQNTHSEKLNFSLIANINKIDTKLFERLLKLWSKNAT
                     INEKYYQ"
     gene            4491..5462
                     /locus_tag="O0394_002266"
     CDS             4491..5462
                     /locus_tag="O0394_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0500333.1"
                     /translation="MKYLLKGNILLLLLILLTIISLFIGVSELSIKDLLHLTESQRNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            5449..6405
                     /locus_tag="O0394_002267"
     CDS             5449..6405
                     /locus_tag="O0394_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCY0500334.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            6402..>6614
                     /locus_tag="O0394_002268"
     CDS             6402..>6614
                     /locus_tag="O0394_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0500335.1"
                     /translation="MIQVENLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFENGEVKIDGQLMSDYKNN"
BASE COUNT         2290 a          942 c         1187 g         2195 t
ORIGIN      
        1 atatccaatg atgtaaacac tacatatagt gatttttata cattcaaccc atataagcta
       61 ctattttctc aaatataaat ctatgcaatt ggtttacatt tgagaaaata agtagcttca
      121 ttatagttaa tacaatgctg agataaccat agtaaccatg ttgttaaagc attttttaat
      181 tggaatgact actttattta aaagggttga agaaagaagg tgatccaatg aaaataatat
      241 atttttcatt tactggaaat gtccgtcgtt ttattaagag aacagaactt gaaaatacgc
      301 ttgagattac agcagaaaat tgtatggaac cagttcatga accgtttatt atcgttactg
      361 gcactattgg atttggagaa gtaccagaac ccgttcaatc ttttttagaa gttaatcatc
      421 aatacatcag aggtgtggca gctagcggta atcgaaattg gggactaaat ttcgcaaaag
      481 cgggtcgcac gatatcagaa gagtataatg tccctttatt aatgaagttt gagttacatg
      541 gaaaaaacaa agacgttatt gaatttaaga acaaggtggg taattttaat gaaaaccatg
      601 gaagagaaaa agtacaatca tattgaatta aataatgagg tcactaaacg aagagaagat
      661 ggattcttta gtttagaaaa agaccaagaa gctttagtag cttatttaga agaagtaaaa
      721 gacaaaacaa tcttcttcga cactgaaatc gagcgtttac gttatttagt agacaacgat
      781 ttttatttca atgtgtttga tatttatagt gaagcggatc taattgaaat cactgattat
      841 gcaaaatcaa tcccgtttaa ttttgcaagt tatatgtcag ctagtaaatt tttcaaagat
      901 tacgctttga aaacaaatga taaaagtcaa tacttagaag actataatca acacgttgcc
      961 attgttgctt tatacctagc aaatggtaat aaagcacaag ctaaacaatt tatttctgct
     1021 atggttgaac aaagatatca accagcgaca ccaacatttt taaacgcagg ccgtgcgcgt
     1081 cgtggtgagc tagtgtcatg tttcttatta gaagtggatg acagcttaaa ttcaattaac
     1141 tttattgatt caactgcaaa acaattaagt aaaattgggg gcggcgttgc aattaactta
     1201 tctaaattgc gtgcacgtgg tgaagcaatt aaaggaatta aaggcgtagc gaaaggcgtt
     1261 ttacctattg ctaagtcact tgaaggtggc tttagctatg cagatcaact tggtcaacgc
     1321 cctggtgctg gtgctgtgta cttaaatatc ttccattatg atgtagaaga atttttagat
     1381 actaaaaaag taaatgcgga tgaagattta cgtttatcta caatatcaac tggtttaatt
     1441 gttccatcta aattcttcga tttagctaaa gaaggtaagg acttttatat gtttgcacct
     1501 catacagtta aagaagaata tggtgtgaca ttagacgata tcgatttaga aaaatattat
     1561 gatgacatgg ttgcaaaccc aaatgttgag aaaaagaaaa agaatgcgcg tgaaatgttg
     1621 aatttaattg cgcaaacaca attacaatca ggttatccat atttaatgtt taaagataat
     1681 gctaacagag tgcatccgaa ttcaaacatt ggacaaatta aaatgagtaa cttatgtacg
     1741 gaaattttcc aactacaaga aacttcaatt attaatgact atggtattga agacgaaatt
     1801 aaacgtgata tttcttgtaa cttgggctca ttaaatattg ttaatgtaat ggaaagcgga
     1861 aaattcagag attcagttca ctctggtatg gacgcattaa ctgttgtgag tgatgtagca
     1921 aatattcaaa atgcaccagg agttagaaaa gctaacagtg aattacattc agttggtctt
     1981 ggtgtgatga atttacacgg ttacctagca aaaaataaaa ttggttatga gtcagaagaa
     2041 gcaaaagatt ttgcaaatat cttctttatg atgatgaatt tctactcaat cgaacgttca
     2101 atggaaatcg ctaaagagcg tggtatcaaa tatcaagact ttgaaaagtc tgattatgct
     2161 aatggcaaat atttcgagtt ctatacaact caagaatttg aacctcaatt cgaaaaagta
     2221 cgtgaattat tcgatggtat ggctattcct acttctgagg attggaagaa actacaacaa
     2281 gatgttgaac aatatggttt atatcatgca tatagattag caattgctcc aacacaaagt
     2341 atttcttatg ttcaaaatgc aacaagttct gtaatgccaa tcgttgacca aattgaacgt
     2401 cgtacttatg gtaatgcgga aacattttac cctatgccat tcttatcacc acaaacaatg
     2461 tggtactaca aatcagcatt caatactgat cagatgaaat taatcgattt aattgcgaca
     2521 attcaaacgc atattgacca aggtatctca acgatccttt atgttaattc tgaaatttct
     2581 acacgtgagt tagcaagatt atatgtatat gcgcactata aaggattaaa atcactttac
     2641 tatactagaa ataaattatt aagtgtagaa gaatgtacaa gttgttctat ctaacaatta
     2701 aatgttgaaa atgacaaaca gctaatcatc tgctctgaat tagcagatga ttagactgct
     2761 atgtctgtat ttgtcaatta ttgagtaaca ttacaggagg aaattatatt catgatagct
     2821 gttaattgga acacacaaga agatatgacg aatatgtttt ggagacaaaa tatatctcaa
     2881 atgtgggttg aaacagaatt taaagtatca aaagacattg caagttggaa gactttatct
     2941 gaagctgaac aagacacatt taaaaaagca ttagctggtt taacaggctt agatacacat
     3001 caagcagatg atggcatgcc tttagttatg ctacatacga ctgacttaag gaaaaaagca
     3061 gtttattcat ttatggcgat gatggagcaa atacacgcga aaagctattc acatattttc
     3121 acaacactat taccatctag tgaaacaaac tacctattag atgaatgggt tttagaggaa
     3181 ccccatttaa aatataaatc tgataaaatt gttgctaatt atcacaaact ttggggtaaa
     3241 gaagcttcga tatacgacca atatatggcc agagttacga gtgtattttt agaaacattc
     3301 ttattcttct caggtttcta ttatccacta tatcttgctg gtcaagggaa aatgacgaca
     3361 tcaggtgaaa tcattcgtaa aattctttta gatgaatcta ttcatggtgt atttaccggt
     3421 ttagatgcac agcatttacg aaatgaacta tctgaaagtg agaaacaaaa agcagatcaa
     3481 gaaatgtata aattgctaaa tgacttgtat ttaaatgaag agtcatacac aaaaatgtta
     3541 tacgatgatc ttggaatcac tgaagatgtg ctaaactatg ttaaatataa tggaaacaaa
     3601 gcactttcaa acttaggctt tgaaccttat tttgaggaac gtgaatttaa cccaatcatt
     3661 gagaatgcct tagatacaac aactaaaaac catgacttct tctcagtaaa aggtgatggt
     3721 tatgtattag cattaaacgt agaagcatta caagatgatg actttgtatt tgacaacaaa
     3781 taacaattaa attaaaagac cttcacatgt aaagggaaat agcgattcgt ttcgtcttgt
     3841 ctcctacatg ttgaaggtct ttttttatgt gtatctaact cattatgagt ctgagtaaga
     3901 aatcaatgct ctaagatgta caatgctatt tatattggca gtagttggcg gggccccaac
     3961 acagaagcag gcggaaagtc agctaacaat attgtgcaag ttggcggggc cccaacatag
     4021 aagcaggcgg aaagtcagct aacaatattg tgcaagttgg cggggcccca acataaaagc
     4081 aggcggaaag tcagctaaca atattgtgca agttcgggcg gggccccaac ataaagaaaa
     4141 actttttcct ttagaaatta tcacttccac atgagtttta ctcatgtatt cctattttta
     4201 agtacacatt agctgaggct aatgttaaga accactactt aatcaatcat tagtagtttt
     4261 tatcatttcc actattccca gacatcaaaa tcttaagtgt tctattttac tttaagtaaa
     4321 caaaatacac attccgaaaa attaaatttc agtttaattg caaatatcaa taaaattgac
     4381 actaaattat ttgaaaggct attgaaatta tggtcaaaaa acgctactat taatgagaaa
     4441 tattatcaat gataatgatt atcattaatt taaagggaga aaaatttgta atgaagtatt
     4501 tattaaaggg aaatattttg cttctattac taatattgtt gacaattatt tcgttgttca
     4561 taggtgtgag tgaactatca attaaagatt tactacattt aactgagtca cagcggaata
     4621 ttttattctc aagccgaata ccaaggacga tgagtatttt aattgctgga agttcgttgg
     4681 ctttagcagg cttgataatg caacaaatga tgcaaaataa gtttgttagt ccgactacag
     4741 ctggaacgat ggaatgggct aaactaggta ttttaattgc tttattgttc tttccaaccg
     4801 gtcatatttt attaaaacta gtatttgctg ttatttgcag tatttgcggt acgtttttat
     4861 ttgttaaaat cattgatttt ataaaagtga aagatgtcat ttttgtaccg cttttaggaa
     4921 ttatgatggg tgggattgtt gcaagtttca caaccttcat ctcattgcgc acgaatgctg
     4981 ttcaaagcat tggtaactgg cttaacggga actttgccat tatcacaagt ggacgctatg
     5041 aaattttata tttaagtatt cctcttttag cattgacata tctttttgct aatcatttca
     5101 cgattgtagg aatgggtaaa gactttacta ataatttagg tttgagttac gaaaaattaa
     5161 ttaacatcgc attgtttatt actgcaacta ttacagcatt ggtagtggtg actgttggaa
     5221 cattaccgtt cttaggacta gtaataccaa atattatttc aatttatcga ggtgatcatt
     5281 tgaaaaatgc tatccctcat acgatgatgt taggtgccat ctttgtatta ttttctgata
     5341 tagttggcag aattgttgtt tatccatatg aaataaatat tggtttaaca ataggtgtat
     5401 ttggaacaat cattttcctt atcttgctta tgaaaggtag gaaaaattat gcgcaacaat
     5461 aataaaaaaa taatgctttt aattgcagta acgttattaa ttagtatgct gtacttattt
     5521 gtaggtattg attttgaaat atttgaatat caattttcaa gtcgtttaag aaagttcata
     5581 ttaattattt tagtaggtgc tgccattgca acttcagtgg tgatttttca agcgattaca
     5641 aataaccgtc tattgacacc atcaataatg gggttagatg cagtttattt atttatcaaa
     5701 gtattgccag tctttttatt tggaattcaa tcggtatggg ttactaatgt atatttgaac
     5761 tttatattaa cacttataac gatggtgtta ttcgcactaa tcctattcca aggtatcttt
     5821 aaaatcggac atttttcaat ttattttatc ttacttattg gtgtcctttt aggaacattt
     5881 tttagaagca taacaggttt tattcaactg attatggatc ctgagtcatt tttagcaata
     5941 caaagtagta tgtttgctaa ttttaatgct tctaattcga atttagttac tttctcagca
     6001 gtgctattag taatcttatt agtcattaca attttactat tgccttattt agatgtattg
     6061 cttttaggtc gtgctgaagc aattaatctt gggatatcgt atgaaaaatt aacgcgaatt
     6121 ctacttgtaa tagtctcagt tttagtttct gtgtcaactg cattagtagg accaattaca
     6181 tttttaggtt tattaactgt aaatctagcg catgaactaa tgaagacgta tgaacataag
     6241 tatattttaa ttgcgacaat ttgcttgagt tggattagtt tatttagtgc gcaatgggta
     6301 gttgaaaatg tgtttgaagc tacgacagaa atgagtatac ttattgattt gattggtgga
     6361 agttatttca tttatctatt agttagaagg agaaatgcgc aatgattcaa gttgaaaatt
     6421 taactaaaac tataaataat caaatgatat tggaagatat tagcatagat atcgaaaaag
     6481 gtaaattgac ttctttaatt ggacctaatg gtgcgggtaa gagtacttta ctttcagcga
     6541 tatgtaggtt aattcgtttt gagaacggtg aagtgaaaat agatggacag ctcatgtctg
     6601 attataaaaa taat
//
LOCUS       DAKTLH010000140         6501 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.142, whole genome shotgun sequence.
ACCESSION   DAKTLH010000140 DAKTLH010000000
VERSION     DAKTLH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.142"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            155..1384
                     /locus_tag="O0394_002269"
     CDS             155..1384
                     /locus_tag="O0394_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCY0500336.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTLAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            1579..2436
                     /locus_tag="O0394_002270"
     CDS             1579..2436
                     /locus_tag="O0394_002270"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCY0500337.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            2436..3380
                     /locus_tag="O0394_002271"
     CDS             2436..3380
                     /locus_tag="O0394_002271"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HCY0500338.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            3649..4617
                     /gene="pfkA"
                     /locus_tag="O0394_002272"
     CDS             3649..4617
                     /gene="pfkA"
                     /locus_tag="O0394_002272"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCY0500339.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            4639..6396
                     /gene="pyk"
                     /locus_tag="O0394_002273"
     CDS             4639..6396
                     /gene="pyk"
                     /locus_tag="O0394_002273"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCY0500340.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
BASE COUNT         2294 a          875 c         1334 g         1998 t
ORIGIN      
        1 attattttat tatcgtttat cgctatttgt gttacataat caaaaccata aattcttact
       61 cattcagatt tacccaatat ttttactttt ataatgtaat gcgttttatc caagttattt
      121 tttaaaaata aatattgaat taggggctga tttcatgtca ttaagagatg aagcattgga
      181 aatgcacaaa cgtaatcaag gtaaattaga agttaaacca aatgtaaaag ttactaataa
      241 agaggaatta agtttagcat actcacctgg cgttgctgaa ccgtgtaaag atatttatga
      301 agataaaaga aaagtatatg attacacaat taaaggaaat acagttgcag ttattactga
      361 tggaacagcg gtattaggtt taggtaacat tggacctgaa gcaagtattc ctgtaatgga
      421 aggtaaagca gtattattca aaagcttcgc tggtatcaat ggggtgccta ttgcgttaaa
      481 tacaactgat accgaagaaa tcattaaaac agttaagttg ttagaaccta attatggtgg
      541 tattaattta gaggatattt cggcaccacg ttgttttgaa attgaagaac gattgaaaaa
      601 agaaactaat attccggtat tccatgacga tcaacatggt acagcaattg taacattggc
      661 aggtttggta aatgcattga gagttgttaa caaagatatt gctaaaataa aagttgtact
      721 aaatggtgct ggtgcagcag gaatagccat tgttaaatta ctatacgcgt atggtgtaag
      781 aaatatggtt atgtgtgact caagaggcgc aatttttgaa ggacgttcat atggtatgaa
      841 tcctacgaaa gatgttgtag caaaatggac aaataaagat aagattgaag ggtctttaga
      901 agaagtcgta aaagatgcag atgtatttat cggggtttct gtagctaatg cgctgtcaca
      961 agatatggtt aagagtatgg cagataatcc aattatattt gcaatggcta atccaaatcc
     1021 tgaaataata cctgatgatg ccaaagcggc aggtgcacga gttgttggta caggacgttc
     1081 agactatcct aaccaaatta ataatgtatt agctttccct ggtattttta gaggtgcatt
     1141 agaggttgaa gctacacata taaatgaaga aatgaaaaag gcagctgtag aagcgattgc
     1201 tgatttaatc gatagttctg aattaaatga agactactgt atcccaggac cgtttgataa
     1261 acgtgtagcg ccatcagttg ctcgtaatgt tgctaaagcg gcaatggaat ctggagtagc
     1321 taggattgaa gttgatccgc aagatgtgta tgataaaaca atgaaactta cagatttaca
     1381 ataaaaataa ttaacgttta attaaattgc ttaaaagtta gcaataaaga gtcattaaaa
     1441 tgatgaaaat gaaagtttat gataaacatt caacagtcaa acgaatataa atcaaataaa
     1501 tttaaacccg tttttaactg gtcaagttca gtttaaggct ctaaatggtt agaacagagg
     1561 ttatttggag gttttcctat gtttaaagat ttttttaatc gaacaaagaa aaagaaatat
     1621 cttacagtac aagactctaa aaataatgat gtgcctgcag gtattatgac taagtgtcca
     1681 aagtgtaaga aaattatgta cacaaaagaa ttagctgaaa atttaaatgt gtgctttaat
     1741 tgtgatcatc atattgcttt aactgcgtat aaacgtatag aagcaatttc tgatgaagga
     1801 tcatttacag aattcgataa gggaatgacc tctgcgaatc cattagattt tccaagttat
     1861 ttagaaaaaa ttgaaaagga ccaacaaaag acaggtctta aagaagcagt tgtgactggt
     1921 acagcacaac tagatggtat gaaatttggc gttgctgtca tggattcacg ttttagaatg
     1981 ggaagtatgg gatcggttat cggtgaaaag atatgtcgca tcattgatta ctgcactgag
     2041 aaccgtttac catttattct tttctctgca agtggtggtg cacgtatgca agaaggtatt
     2101 atttccttga tgcaaatggg taaaaccagt gtatctttaa aacgtcattc tgacgctgga
     2161 ctattatata tatcatattt aacacatcca actactggtg gtgtatctgc aagttttgca
     2221 tcagttggtg atataaattt aagtgagcca aaagcgttga taggttttgc aggtcgtcga
     2281 gttattgaac agacaataaa cgaaaaattg ccagatgatt tccaaactgc agaattttta
     2341 ttagagcatg gacaattgga taaagttgta catcgtaatg atatgcgtca aacattgtct
     2401 gaaattctaa aaatccatca agaggtgact aaataatgtt agattttgaa aaaccacttt
     2461 ttgaaattcg aaataaaatt gaatctttaa aagaatctca agataaaaat gatgtggatt
     2521 tacaagaaga aattgacatg cttgaagcgt cattggaacg agaaactaaa aaaatatata
     2581 caaatctaaa accatgggat cgtgtgcaaa ttgcgcgttt gcaagaaaga cctacgaccc
     2641 tagattatat tccatatatc tttgattcgt ttatggaact acatggtgat cgtaatttta
     2701 gagatgatcc agcaatgatt ggtggtattg gctttttaaa tggtcgtgct gttacagtta
     2761 ttggacaaca acgtggaaaa gatacaaaag ataatattta tcgaaatttt ggtatggcgc
     2821 atccagaagg ttatcgaaaa gcattacgtt taatgaaaca agctgaaaaa ttcaatcgtc
     2881 ctatctttac atttatagat acaaaaggtg catatcctgg taaagctgct gaagaacgtg
     2941 gacaaagtga atctatcgca acaaatttga ttgagatggc ttcattaaaa gtaccagtta
     3001 ttgcgattgt cattggtgaa ggtggcagtg gaggtgctct aggtattggt attgccaata
     3061 aagtattgat gttagagaat agtacttact ctgttatatc tcctgaaggt gcagcggcat
     3121 tattatggaa agacagtaat ttggctaaaa ttgcagctga aacaatgaaa attactgccc
     3181 atgatattaa gcaattaggt attatagatg atgtcatttc tgaaccactt ggcggtgcac
     3241 ataaagatat tgaacagcaa gctttagcta ttaaatcagc gtttgttgca cagttagatt
     3301 cacttgagtc attatcacgt gatgaaattg ctaatgatcg ctttgaaaaa ttcagaaata
     3361 tcggttctta tatagaataa tcaacttgag catttttatg ttaaatcgat actgggtttt
     3421 accataaatt gaagtacatt aaaacaataa tttaatattt agatactgaa ttttaactaa
     3481 gattagtagt caaaattgtg gctactaatc tttttttaat taagttaaaa taaaattcaa
     3541 tatttaaaac gtttacatca attcaataca ttagttttga tggaatgaca tatcaatttg
     3601 tggtaattta gagttaaaga taaatcagtt atagaaaggt atgtcgtcat gaagaaaatt
     3661 gcagttttaa ctagtggtgg agattcacct ggaatgaatg ctgccgtaag agcagttgtt
     3721 cgtacagcaa tttacaatga aattgaagtt tatggtgtgt atcatggtta ccaaggattg
     3781 ttaaatgatg atattcataa acttgaatta ggatcagttg gggatacgat tcagcgtgga
     3841 ggtacattct tgtattcagc aagatgtcca gagtttaagg agcaagaagt acgtaaagtt
     3901 gcaatcgaaa acttacgtaa aagagggatt gagggccttg tagttattgg tggtgacggt
     3961 agttatcgcg gtgcacaacg catcagtgag gaatgtaaag aaattcaaac tatcggtatt
     4021 cctggtacga ttgacaatga tatcaatggt actgatttta caattggatt tgacacagca
     4081 ttaaatacga ttattggctt agtcgacaaa attagagata ctgcgtcaag tcacgcacga
     4141 acatttatca ttgaagcaat gggccgtgat tgtggagatc tagcattatg ggctggatta
     4201 tcagttggtg ctgagacaat tgtagttcca gaagtgaaaa cagatattaa agaaatagct
     4261 gataaaattg aacaaggtat taaacgtggt aagaaacact caatcgttct tgtagcagaa
     4321 ggttgtatga ctgcgcaaga ttgtcaaaaa gaattatcac aatacatcaa tgttgataat
     4381 agagtgtctg tgttaggtca cgttcaacgt ggtggtagcc caacaggtgc ggatagagtt
     4441 ttagcatcac gtttaggtgg atatgcggta gacttattaa tgcaaggtga aacagctaag
     4501 ggtgttggaa ttaagaacaa taaaattgta gcaacatctt ttgatgaaat ttttgatggt
     4561 aaagatcata aatttgatta tagtctatat gaacttgcta acaagttatc tatataagat
     4621 ttcaggagga attataaaat gagaaaaact aaaattgtat gtacaattgg accagcttca
     4681 gaatcagaag aaatgattga gaaattaatc aatgctggta tgaacgttgc acgattaaac
     4741 ttttcacatg gtagtcatga agagcataaa ggtagaattg atacaattcg taaagtagct
     4801 aaaagattag acaaaattgt agcaatttta ttagatacaa aaggtccaga aattcgtacg
     4861 cataatatga aagacggtat cattgaactt gaacgtggca acgaagttat tgttagcatg
     4921 aatgaagttg aaggaacacc tgaaaagttc tcagtaacat atgaaaactt aattaacgat
     4981 gttcaagtag gttcatacat tttacttgat gatggcttaa ttgaattaca agttaaagat
     5041 attgaccatg ctaaaaaaga agttaaatgt gatattttaa actctggtga gcttaaaaac
     5101 aaaaaaggtg ttaacttacc tggcgtaaga gtaagtttac ctggtattac agaaaaagat
     5161 gctgaagata tccgtttcgg tattaaagaa aatgttgact tcattgcagc aagtttcgta
     5221 cgtcgtccta gtgatgtttt agaaattcgt gaaattttag aagaacaaaa agctaacatt
     5281 tcagtattcc ctaaaattga aaaccaagaa ggtattgata atattgcgga aattcttgaa
     5341 gtgtctgatg gtttaatggt tgcacgtggt gacatgggtg ttgaaattcc acctgaaaaa
     5401 gtaccaatgg ttcaaaaaga tttaatcaga caatgtaaca aattaggtaa accagttatt
     5461 acagctacac aaatgttaga ttctatgcaa cgtaacccac gtgctacacg tgcagaagct
     5521 agtgacgttg ccaacgcaat ctatgatggt acagatgcag taatgttatc tggtgaaact
     5581 gctgctggtt tatatcctga agaagctgtt aaaacaatga gaaatattgc tgtatcagct
     5641 gaagcagccc aagattacaa aaagttattg tcagatcgta ctaaattagt tgaaacttca
     5701 ttagtgaatg ctatcggtat ttcggttgca catacagctt taaacttaaa tgttaaagca
     5761 attgtagctg ctactgaaag tggttcaacg gcacgtacta tctccaaata tcgtccacat
     5821 tcagacatta ttgcggtgac tccaagtgaa gaaactgcac gtcaatgttc aattgtttgg
     5881 ggagttcaac ctgtagttaa aaaaggacgt aagagtacag atgcattgtt aaacaatgca
     5941 gttgcaacag ctgttgaaac tggtagagta tctaatggtg atttaatcat tattactgct
     6001 ggtgtaccaa ctggtgaaac tggaactact aatatgatga aaatccacct agttggtgac
     6061 gaaattgcta atggtcaagg tattggacgt ggatcagttg ttggtactac gttagttgct
     6121 gaaactgtta aagatttaga aggtaaagat ttatctgaca aagttatcgt tactaactca
     6181 atcgatgaaa cgtttgtacc ttatgtagaa aaagctttag gcttaattac agaagaaaat
     6241 ggtattacat caccaagtgc aattgttggt ttagaaaaag gtattccaac agttgtaggt
     6301 gtagaaaaag ctgttaaaaa cataagcaat aacatgttag ttacgattga tgctgctcaa
     6361 ggtaaaatct ttgaaggata tgcaaacgta ctataattta taaaaaaacg tctttccatt
     6421 tatcaacaat ggaaaggcgt tttttggttc atctggtatt ttatgacgta attaataggt
     6481 tatttgataa tgatagtgta t
//
LOCUS       DAKTLH010000141         6493 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.143, whole genome shotgun sequence.
ACCESSION   DAKTLH010000141 DAKTLH010000000
VERSION     DAKTLH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.143"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..222)
                     /locus_tag="O0394_002274"
     CDS             complement(<1..222)
                     /locus_tag="O0394_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0500341.1"
                     /translation="MIKLNFNDLEIFITVCEEASINKAAIKLRYAQSNISQRISKLEN
                     ELGVVLLFRNQKGAKATKAGEEFLAYSKKV"
     gene            313..846
                     /locus_tag="O0394_002275"
     CDS             313..846
                     /locus_tag="O0394_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HCY0500342.1"
                     /translation="MHAMQYHVKLPSDYNMEIIRDRVRLNGYKTDGFKNLIIKAYLIS
                     QTTTNCITNTYSPLYLWRSSEGMTEFIFNGFYDNVISSFGWQNINIGVIYSMNITDSV
                     KHSLYALEEYIDIFPTLSLKEIEIKKLFRIFDNAVAEIIIYNPDKWKFVKYSFIENLP
                     KSLNPNIKIYDVLYISQ"
     gene            complement(1201..2061)
                     /locus_tag="O0394_002276"
     CDS             complement(1201..2061)
                     /locus_tag="O0394_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0500343.1"
                     /translation="MNLNILKSFLVTSETKNLTKASELLNYSQSTVSTHIEKLERQLD
                     VKLFYRKKYGMELTEEGLAYVKYAKVILDSNSEYEREIKGLYNKKVNISINMQESQYL
                     YRYYNKISEWLAEHPYVNLKFKSAHSNFYIKEEIANFKSDISLITDEKIINSNLTAIP
                     ITEERLIFVTNKEMKDFKLNDIQNHTLLVTEKGCSYREQLEMILYKYSFSTKQMIEFL
                     GIESLKKHLKNSGGIALLPEFIVADELENKSLFQIDVDVDIPNLETTLIINPESNKQV
                     LESFVKDVFL"
     gene            2174..2724
                     /locus_tag="O0394_002277"
                     /pseudo
     CDS             2174..2724
                     /locus_tag="O0394_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
     gene            2717..3937
                     /locus_tag="O0394_002278"
     CDS             2717..3937
                     /locus_tag="O0394_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0500344.1"
                     /translation="MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQL
                     LGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETY
                     LVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGIL
                     YKINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNM
                     MIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAI
                     LSKKISTKVLIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMN
                     TVSDKYVGRSFTIWTSISLLLQSLIAPFLGRWINEINDKFGFYIILILSLLIFVTLLL
                     VNKTGKIKYAHKEE"
     gene            complement(4009..4305)
                     /locus_tag="O0394_002279"
     CDS             complement(4009..4305)
                     /locus_tag="O0394_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HCY0500345.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKRRKMMGEYIIYYDGVVIGGLYDN
                     RLLVKATKSAQQKLQDNTLVSPYPGSKEMILILDFTEATNLTDLFKTIKNDLKK"
     gene            complement(4354..4557)
                     /locus_tag="O0394_002280"
     CDS             complement(4354..4557)
                     /locus_tag="O0394_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500346.1"
                     /translation="MEGLKHSLKSLGWWDLFFAIPIFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            4866..5852
                     /locus_tag="O0394_002281"
     CDS             4866..5852
                     /locus_tag="O0394_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCY0500347.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL"
BASE COUNT         2249 a          986 c          960 g         2298 t
ORIGIN      
        1 tacttttttg ctatacgcta agaattcttc gcctgcttta gttgccttag cacctttttg
       61 atttctaaaa agcaaaacta cacctaattc attttcaagc ttgctaattc tttgagatat
      121 attagattgt gcatatctaa gtttaattgc agctttattg atagatgctt cttcacatac
      181 agttataaaa atttccaaat cattaaagtt caatttaatc acctatcact ttttatgata
      241 actatatcac aattggataa tatgagattt tacttattat tgctatcctt tttataaaag
      301 ggggtatgct aaatgcatgc aatgcaatat catgttaagt tgcctagtga ttacaatatg
      361 gaaataatta gagatagagt tcgattaaat ggttataaga ctgatggatt taaaaattta
      421 attataaaag cttatttaat aagtcaaaca actactaatt gcattacaaa tacttattca
      481 ccattatatt tatggagaag ttcagagggg atgactgaat ttatattcaa tggattttat
      541 gataatgtta tttcttcttt tggatggcaa aatataaata ttggtgtgat atattcaatg
      601 aacataaccg attctgttaa acatagtctt tatgcgcttg aagaatatat cgacattttc
      661 cctactttat ctctaaaaga aatagaaatc aaaaaattgt ttagaatctt cgataatgca
      721 gttgcagaaa ttataattta taatcctgat aagtggaagt ttgtgaaata ttcatttatt
      781 gaaaatcttc caaagagtct caatccaaat ataaaaattt atgatgtact atatatctca
      841 caatgaaagt acagatttaa agttattatt atcaacatta gaaaacagac taaaataatt
      901 tatctgaact tattaataat aaagaacctg ctagatgaca gctatttaaa atacataagc
      961 tactcattta gcaggttaaa atattaatca atccatacta actagtatga ttcaaaggtt
     1021 ttcatctatt aaattcttta ctattttgaa aatgaatgcc cgcaatttga aatttcaact
     1081 agaaataatc cttattaaag atataaatca cctgttttta tattgaatta ctttttcatt
     1141 aacatctcat ttaaattatt atctttgagt ttcaccataa ctacacgttt tcaccaataa
     1201 ttataaaaaa acatctttta caaaagattc aagtacttgc ttattcgatt caggattaat
     1261 tatcaatgtt gtttctaaat tcggaatatc gacatctacg tctatttgaa acaaactttt
     1321 attttctaat tcatctgcaa caataaattc cggcaatagc gcaatcccac ctgaattttt
     1381 caagtgtttt tttaatgact caattcctag aaattcaatc atttgttttg ttgaaaagct
     1441 atatttatat aaaatcattt ctaactgttc tctgtaacta cacccttttt cagtaacaag
     1501 taatgtatgg ttttgaatat cgtttaattt gaaatctttc atctctttgt tagtaacaaa
     1561 tattaaacgt tcttcagtta taggaatagc agttaagtta ctattaataa tcttttcgtc
     1621 tgtgataagg ctaatatccg atttgaaatt agcaatttct tctttaatat agaaattaga
     1681 atgtgcggat ttaaacttca agtttacata tgggtgttca gctaaccatt cactaatctt
     1741 attatagtag cgatacaaat actgactttc ttgcatgtta atacttatat ttaccttctt
     1801 attgtaaagt ccttttattt ctctctcgta ttcgctatta ctatctaaaa tcactttagc
     1861 gtatttaacg tatgctaagc cttcttccgt tagttccata ccatattttt ttctataaaa
     1921 taatttaaca tctaattgcc tttctaattt ttcaatatgt gtagatacag ttgactgtga
     1981 atagttaagt aactctgatg ctttagtaag gttctttgtt tcactagtta ctaaaaatga
     2041 ttttaaaatg tttaagttca aagtagtacc tcctttaggg ttccctttta tcgaatcctg
     2101 aattctaaga attctgattt aataataata tattaaatat tattataaag gtaaatcgaa
     2161 aggagttatg actatgatta cttgtttatt tggaagtagt agaaaggtgg caattcagct
     2221 atcgcagtag agaatctatt gaagaattta aaagtcaact ttgtgaattt atatcaaagc
     2281 aacattgaaa aggttacaga taatcgtcat agcaaaggtg ccaactttca taatgatgat
     2341 tacgaacaaa ttttaatcaa agttttagaa tcagatatta ttattttttc aacaccactc
     2401 tattggtact ccatgagtgc gtctttaaaa ctcttcattg atagatggac cgagtcattg
     2461 cgagatacgc aaatagacaa cttcaaagaa atcatgtcac aaaaaaagta tttgatttta
     2521 atcataggtg gcgacaatcc gcataccaaa gcacaacctt tagtcaacca attcaaatta
     2581 atatttgagt ttatgaatat tactaactat agatttttga tcggcgaagg caataaacca
     2641 ttcgatattt taaacgactc tcaatttata gaagagctcg caaatataaa tcttgcattg
     2701 aaaaaaggtg atatttatga ctgatttgtt gaaaattaaa aacttcagat tgttcttttt
     2761 agcagaaata atttcagcat tcggagttgg aattagtaca gttggggcta attggtattt
     2821 aatcgacaaa actaacgact cccaactatt aggtattatg ttagctttaa atgtattatc
     2881 cggtttctta gcttctccta taataggtgg actagccgat aaatataata gacgaaatat
     2941 tattttaatc acctatcttc ttcaggtaat attatattta ctcattgtca tagcactcgt
     3001 aatgatagga tttgagacct atttagtgat tggatttgcg attgttaatg ggataggttg
     3061 gactacgtat atggcaacat ctagaagttt agttaaacag atattaaaac cagatcaata
     3121 tacagatgca aattctctat tagagattag tttgcaaaca ggtatgttta ttgcaggtgg
     3181 attatcagga atattatata aaataaatgg attcactcta attatagcga tgactataat
     3241 gatgtttcta atcagcattt ttatgttatt tagattgcat gtagataaac caactcattc
     3301 agaggaagaa tcaacaaata gcttattgca agaatattta ttaggatgga aatttttaaa
     3361 agacaacatg atgatattta tttttggagt tatttcaatc ataccaatgg tgtttacaat
     3421 gatctttaac atatcattac caggatatgt ttataatgtt ttaaaacttt cgtctgtaca
     3481 atttggtttt tcagatatgt tatatggcat tggaggatta tgtgcaggtt taatttcagc
     3541 tattctttcg aagaaaatct caactaaagt attgatattt ttgttatatt tcatattagt
     3601 cataaattca gcactattta tttggataaa ctcagcattt tacttattca taggatcatt
     3661 tatactaggc tactcaattt catcaatcag aatttatatg aatacagcta taatgaacac
     3721 tgtttcagat aaatatgtcg gtcgctcatt tacgatatgg acgtcaattt cattgttact
     3781 gcaaagttta attgctccat ttttaggaag atggattaat gaaattaatg ataaattcgg
     3841 tttctatatt atactcattt tatccttact catatttgtt acactgctgc ttgttaacaa
     3901 aacaggcaaa ataaaatatg cacataaaga agagtgaccg tcactcttct ttaacaagcg
     3961 accatttatc gatgggctta gttctctctg cacccacact tcactacttc actttttcaa
     4021 atcatttttt atggtcttaa ataaatcagt gagatttgtt gcttcggtaa agtctagaat
     4081 taatatcatt tctttagaac ctggatatgg cgaaactaat gtattatctt gcaatttctg
     4141 ctgggcactt ttagtcgcct tgaccaataa tctattatca tacaaaccac ctataaccac
     4201 gccatcataa taaataatat attctcccat catctttctt ctcttaaccg cgtttgaatt
     4261 cacatgattt aaaaataaat catgtacatc tttcttagtc gccattgtaa tcgctccttc
     4321 agttttatgt ttaatcacat tcgtattaat gattcatttc gtgttgctct taattttatc
     4381 tataattata tgcgtagtta aaatcaaacc tatggaaaag aaaataatga taacaatgtt
     4441 aagaaatata gttataaaat tatagtttgg aaggtatgcg aatagcagaa aaataggtat
     4501 cgcaaaaaat aaatcccacc aacctaaact ttttaaagaa tgctttaaac cttccataat
     4561 atcaccttta taaatttgtc tttgttataa gataactaaa aaatcgcttt actgtaaaag
     4621 tagccaaaga aaattctgaa tcatattcat aagtagtgta tcattaataa tgaacaattt
     4681 aatactataa tccttgatct ttgtattgat caacttacca caacatttat tttagactac
     4741 tcttagactt ccctttcaaa tggttgcatc tattgaaatt ccttttgtat aagttaggct
     4801 tttgtggtaa tatcatcatg cataaaaaat cgagatacta attataaaga gggtataaat
     4861 atattatgaa agaaaatttt tggagtgaat taccacgtcc attttttatt ttggcgccaa
     4921 tggaagacgt tacagatatc gtctttcgac acgttgtaag tgaagcagct agaccggatg
     4981 tgtttttcac tgaatttaca aatactgaaa gcttttgcca ccctgaaggc atacatagtg
     5041 tgcgcggacg cttaactttt agtgaagatg aacagccgat ggtcgctcat atatggggag
     5101 ataagccaga acagttccgt gaaacgagta ttcaattagc taaaatgggc tttaaaggca
     5161 tagacttaaa tatgggatgt cctgtagcaa atgttgctaa aaagggtaag ggttccggct
     5221 taatcttaag acctgacgtt gctgccgaaa ttattcaagc gactaaagca ggtgggcttc
     5281 cggtaagtgt taaaacacgc cttggctact atgaaatcga tgaatggaaa gattggttga
     5341 agcacgtctt cgaacaagac attgccaatt tatctattca tcttcgtaca cgtaaagaaa
     5401 tgagtaaagt agatgcacat tgggaattaa tcgaagctat taaaaattta cgtgacgaaa
     5461 ttgcaccaaa tacattgtta acaattaacg gtgatattcc cgatagaaaa acaggacttg
     5521 aactggcaga aaaatatggc attgatggcg tcatgattgg tagaggcatt ttccacaatc
     5581 cattcgcttt tgaaaaagaa ccacgcgaac acacaagcaa ggaactatta gatctattga
     5641 gattgcattt atcattgttt aacaaatatg aaaaagatga aatacgacaa ttcaagagct
     5701 tgcgtagatt ctttaaaatc tatgtgcgtg gcataagagg cgctagcgaa cttcgacatc
     5761 aattgatgaa cacacaatca attgcagaag cacgagcact actcgatgaa tttgaagccc
     5821 aaatggacga agacgttaaa attgaattat agtatgagta ataaagttta tggatgatat
     5881 ttcccaattt aacacggatt gaacactttc tacttaggta ttatcttggt tttcctgata
     5941 ggataactcc cgatgatgcc tttttactta tgtcacttat ccttttggaa atggtgcaaa
     6001 attaaaaata catcaggata tcgtttaact cagaaaaaaa ctccaatcaa aaaaaatgtt
     6061 tacaacacat tatgaaatta aaattgctct tagtgttcaa cagacaaagt tattgaaatt
     6121 actattggag gtacatacca agctatcatt ttgagtacac gtggtttgaa tatctggcaa
     6181 gaaatggaaa ttggtaatgt cagtcataaa gtcgctaaac gtgctcaaat accattaatt
     6241 atttttaaat aatttagagg atgaggtata aacctttaaa aaacagcagt gagatgattt
     6301 tcaattagaa aatatcttac tgctgtttct attttcataa tcattcttat tgaatgactt
     6361 catcgtgatc atttaaaaca gtaacaactt tttcaatctt atactattta gagctaaaaa
     6421 ttcacgacat ataaacacaa cactgacacg tcatttaaat acttaatctt ttatagtaaa
     6481 agatttttca tta
//
LOCUS       DAKTLH010000142         6422 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.144, whole genome shotgun sequence.
ACCESSION   DAKTLH010000142 DAKTLH010000000
VERSION     DAKTLH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6422
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.144"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(74..766)
                     /locus_tag="O0394_002282"
     CDS             complement(74..766)
                     /locus_tag="O0394_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCY0500348.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFNFADVTKEKCIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKI"
     gene            1002..1460
                     /locus_tag="O0394_002283"
                     /pseudo
     CDS             1002..1460
                     /locus_tag="O0394_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1447..1749
                     /locus_tag="O0394_002284"
     CDS             1447..1749
                     /locus_tag="O0394_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500349.1"
                     /translation="MRTNDHITDLDAIELSSGWNYNSKLETGKNALLSDSLLRDIKVL
                     SSNAKGDADILETELRAVINSMSQNEGKSVDALKNSTASLKNNLTKLKEQLDKTTH"
     gene            1903..2262
                     /locus_tag="O0394_002285"
     CDS             1903..2262
                     /locus_tag="O0394_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500350.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALNL
                     LGLATLSLLDTFSCNKK"
     gene            complement(2347..2958)
                     /locus_tag="O0394_002286"
     CDS             complement(2347..2958)
                     /locus_tag="O0394_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCY0500351.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTELSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGIFTIKRPEK"
     gene            complement(3037..3513)
                     /locus_tag="O0394_002287"
     CDS             complement(3037..3513)
                     /locus_tag="O0394_002287"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HCY0500352.1"
                     /translation="MAVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGQKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(3516..4016)
                     /locus_tag="O0394_002288"
     CDS             complement(3516..4016)
                     /locus_tag="O0394_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0500353.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATREALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSTNNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFETLAKNHHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(4251..5018)
                     /locus_tag="O0394_002289"
     CDS             complement(4251..5018)
                     /locus_tag="O0394_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HCY0500354.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILKGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(5015..5707)
                     /locus_tag="O0394_002290"
     CDS             complement(5015..5707)
                     /locus_tag="O0394_002290"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HCY0500355.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNRDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(5724..6386)
                     /locus_tag="O0394_002291"
     CDS             complement(5724..6386)
                     /locus_tag="O0394_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HCY0500356.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNKISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
BASE COUNT         2110 a         1167 c          918 g         2227 t
ORIGIN      
        1 gcgaaaaaat gcatcgctac taagttgaat gtttagtaag gatgcattta attcactaaa
       61 atgattaaat tacttatatc ttttcatctg attgattatc gaaatttctt ccttctaaac
      121 ctgctaactc ttctttagaa gctgcaggtg ctttcatttc aaatatctca ttcactactg
      181 tgtaatcgta atatcctaat ctggcaatag gtttaatcga cttaatgtcc aatttaccat
      241 tatcaagaat aaccttatcg tcaatatgaa cttgggcaac tcttcctata acaatatcta
      301 cggtagatac tggatctcca gttggaatac gaatcgtttg aacgtactca cattcaaaat
      361 gaactggcga ttcttttaca cgatatcctg gagcttctat acatttttcc tttgttacat
      421 ctgcaaaatt aaattcatcc tcttctggtg gcaatgcttt cgatgataaa ttaactgctt
      481 ctcttaaatc atacgttgcc atattccaca caaaccaacc tgtctcttca gcatttttca
      541 ctgtatcttt acgttcgtga tcaccaagaa cggattgatt tgctgcgaac ataaccatag
      601 gcggatccca agttaagttt tgatactgac tataaggcgc taaattatct ttcccatctt
      661 tcgatacagt agagatccac cctattggac gtggtactgt actactttta aatgggtcgt
      721 gcggtaaacc atgacttctt acaccttgtt ttggcgaata attcatacta tcttcacccc
      781 ttataagtaa ttacatttaa ggttacgccc tctttacata agcgtctaat ataaataaac
      841 aatttattta taagtagaaa ctatatatga cgtggttgct tataatttgc gttcttgatt
      901 cgaaaaattc agataaggat ttatacaatt aatatttatg atatcttttg taaatttaat
      961 taattatagt tacttcaatc atgattagtt tataataata aagtgaaatt gaaaaagaca
     1021 gctattatgc gatgagcgaa aaacttcaag taaaacaaga atttaacggc gttacaaggc
     1081 aatacttgaa tttcagaaca gatagtatcg atgatctgga taagttcaaa aaagaattca
     1141 aacctgttat gcacttaaaa ggtgatgcat tcaatcaaca gttacaatct ttgattaaca
     1201 aatatccaca aatacaaaaa aatatgaaat cagagttcat tgcttattat gataaagaaa
     1261 aaaatagaga aacagtaaaa aactatgctt ggaaccttca aaaatctata aatgacatta
     1321 tgcaatcata tcctagcaca aaatttgtac agttttataa agatgatgtt tccccgtcaa
     1381 tggtagatgg aaatggccgt ttaaaatcgg atactaatgt aatttccatc gagggaggaa
     1441 agtatgatga gaacaaatga tcatataacc gatttagatg ctattgagtt aagtagcggt
     1501 tggaattaca attcgaaatt ggagactggc aaaaatgcac tactatcaga tagcctttta
     1561 agagatatta aagtattaag ttctaatgct aaaggcgacg ctgacatact agaaacagaa
     1621 cttcgtgctg taataaactc aatgtctcaa aacgaaggta aatctgttga tgcacttaaa
     1681 aatagtaccg cttctctaaa aaacaatcta accaaattaa aagagcaact cgacaaaact
     1741 acacattaat ttcatacatt ttgagtgaac agaattatat ttccatagca aacattccta
     1801 aactacacat acgttacata attgattcat ttttatagaa acgggtaaaa atgataaagt
     1861 aatcatcttt cacaaaaaat caaatattgg gaggaattaa atatgatttt gcgttacgca
     1921 acgaatttaa aacttgcgaa agagctatac caagccagta aacctaaatt gcaagaagat
     1981 aagggtatga ttgaactgtt tgaacatact tttggcttgc aaaaagaact tgtaaaatac
     2041 gtcggcattg cagaagcaac tactgctgct ttatactcag ctagctttat caataaaaac
     2101 atttctcgcc ttgcatcact atcaacgata ggcattttaa gtgtagctgc ttacaaacat
     2161 ttcgaagctg gccaaggtaa aaaaggtgca caacatgcat taaatttatt aggtcttgct
     2221 actttaagtt tactagatac attttcttgt aataaaaaat agtcttcatt ttagaagcac
     2281 atactttagc acctgtgtac ttttgattca attcataaac ggtataccgc tatagcatgt
     2341 gttttattat ttttctggtc tctttatcgt aaatattccc cctaatttag cataatcatt
     2401 tccggctaaa cgagaaacag cctgtaatgc gtgtggattg attttaaaat caccctcata
     2461 aatactgtca tcaatatgat aagttacaat ttcacctatt attaaatcag ccccatctaa
     2521 tacatctcca agcaatatca tttgcgatag tttacattcg aatctcattt tcgcatcttt
     2581 aattcctggc gtcttaatcg ttgtagatgt taaaagtgat aattctgtac gactcaactc
     2641 actgtcacca tatgctaacg gcgctgcagt ctcattaata tcttgaacat tatcttcgtc
     2701 tgtaatatgc acaacaaagt ctccagtccg ttctatattt aatgcagtat cttttctctt
     2761 acctcctgca cgttgaactg caatagcaat cattggcgga tgattattaa caatattaaa
     2821 aaagctaaat ggtgctgcat ttactgatgc atcttgattt aatgttgtaa caaaagctat
     2881 aggtcgtgga ataattgaac caattaataa tttatagttt tctctagcag ttaatgattg
     2941 tgcatcaaac gtatacataa tacctacctc ttttctaagt atatctaggt atttctccga
     3001 ttttggttaa tttaaacatc tattctcctc tgaaaatcac ttgtatttat ttagcaaatc
     3061 ttttgaaata tgacacatat gcatatcttc tggatatttt tctaaatgtt gctgatgttc
     3121 ttcagcactt ttaatgtagt tagacagcgg taagacttcc actgcaattt gatctctgtc
     3181 tttacgtcgt tcaatgaact gacgcgcttc aattaagtgg tcatctacac aactatataa
     3241 acccgttcga tacttttgtc caatatcatt tccttgttga ttcacactgt aaggatcaat
     3301 gatttcaaat aaataattca taatgtctgt aattgttaac atacgatcat cgaaatgaag
     3361 tttgacacat tcagcataac catcatacgg accgtctaat ttagagcttc ttccatttgc
     3421 tcttcctgct tctgtatgta taattccagg tattgttgca aaaaatgctt caacacccca
     3481 taaacatcct cctgctacat aaacaactgc catatttaca cctcatcatc cttttttata
     3541 tttttaacaa ggttatacca tttaataccg ccatgacatg attctgatac accttcatta
     3601 cgatacccat atttttcata aaatgaaatt aatgattctc gacatgttaa cgttacacca
     3661 tgtcgatgat gattcttagc aagagtttca aaatagttta gtaagcgacc tgcaataccc
     3721 tgaccttgat aatttggtgc tacaacaaga cctaacacac taatatagcc accttcacta
     3781 ttatttgtgg agacattttt aaataaatca tcgctaatgt aacgctcttt tatgactgga
     3841 ccgttgatgt atccaattac ttcatcattc ttttcagcta caataaaagt ttcttgaatt
     3901 tgttcaattc tcaatttcaa tgcctctcgt gttgcagctt cttccggtga aaaaccaata
     3961 ttttcaagtg caataatttg atctaagtcc tgcatgatta cttttcttaa taccataaaa
     4021 acacttcctt agccctatat atcaaaagta ataccttcat ctttatattc attattttca
     4081 aactaccttc actataaaaa acgagtcttt ttattgaaag ccattcgcct taatcaatta
     4141 gacaagttgt attatacgaa ttagtaaata atcatgatta taattcattt taagcatact
     4201 caaaaagact ggtacatgta taccagtcta attcgaaaaa atatatttag ttaaaaccat
     4261 ttcaaaaacg acttcacttt tcctggctta gtcggtctgt ctaaataaac aaaattatca
     4321 ttgattactg aactggcatt tcgaagtaat gcttcaatat cattcgaata tttcttcaat
     4381 ttatgattgt gaaataattc ttgcatcaaa aatggtcttt ggtcacatga atgtgcatct
     4441 gaagctacaa aatgagccaa attacattct ataaattgta atgataactt ttgaatgttt
     4501 ttaccaaatc caccaactaa agaactcgat gttaattgac tcagtgcccc atttgcaacc
     4561 aattcatata atatttccgg atttttggcg atacttctat ttctttcagg atgtgcaatg
     4621 attggtatgt aacctctcga ttgtatttca aaaaacaatt gttttgtata atgtggtact
     4681 tcgcccgttg gaaattcaat taataaatat ttcgaacgat taataccttg aatactacca
     4741 ttatctaagc ctttcagaat cgaatctgta attctaattt cttgcccggg aaataattta
     4801 atatccaatg cttgaacttc tggatgcgtt cttaactccg ccaatttcac aagcacttgt
     4861 tgaaatgtat tatcatatct cggatgcaaa tgatgaggtg tcgctacaat acttgttaca
     4921 ccttcatcct tagcttgctt taatagtgca atactctttt caattgtttt aggaccatca
     4981 tctatatcaa ctaatatatg gttatgaata tcaatcatga ttcatcagtc ccataatatg
     5041 catagtaact agcactttta tctttaggca ttctatttaa gactacacct aataatttag
     5101 cacctgttgc ttcaataagt tcttttcctt ttttaacttc atctctatta ttattttccg
     5161 aattaactac gtagacaaca ttgccggtaa actttgaaaa taattgcgca tctgtaactg
     5221 tgttcactgg tggcgtatcg ataattacaa agttataatt catcaataat gtgtcataca
     5281 aatttgcaaa tgcccttgat gtaattaact ctgacggatt cggtgggatt ggcccagacg
     5341 tcaagacgtc taaatcttga atttcagttg agataatact gtcttgataa gttgaccaat
     5401 ttagcaataa acttgatagg ccttcattgt ttggcaaatt aaaaatataa tgctgcgtag
     5461 gtttacgcat atccccgtct acgattagtg ttttataacc tgcttgcgca tatgcaactg
     5521 ctaaatttgc tgcaattgta gacttacctg cgcctggtgc ctctgaagtg attacaatgc
     5581 tttgaactgc actgtcagga tttgcaaaca taatatttga tcttatgcct cgaaatttct
     5641 cgctaatagg tgactttggt tgttcatgga caattaaact tgatgtactt cttcgtgtat
     5701 tcgtcatggt aattcctcgt aaattaaaat ttttgtattg aacctaaaat aggtaatcct
     5761 agttgcgatt caacatcttc ttctgtctta atacgcttat ctaataattc ttttaagaaa
     5821 ataatcaata ttgctaaaac aataccaaca ataatgctga taactaagtt gacagatact
     5881 attggagata cttttacagc attatcatgt gctgaggaaa gtatcgtaac attatcaaca
     5941 ctcataattt taggcatgtc atgagcaaaa actttagata ttttattaac aattttgtca
     6001 gattcagatt tattcccagt ggtaactgat acagtaataa tttgagagtt tgtttgattg
     6061 gttactttta aaaatgaatt caactcagct gttgaatact gaccatcaaa ttctctagat
     6121 actttatcta gaattctagg acttttgata atttccgtat atgtattaac agactgcaaa
     6181 ctactttgaa cattttggaa agctaaatca cttgaggact ttttcatgtt cactaatatt
     6241 tgagtagaag cagtatattt gtcaggcata acaaaaaagg ttaatgccgc acttactaca
     6301 agacatattg ccggtaaaat aagcaataat ttaatattct tctttagaat atttaatagt
     6361 tttactaaat caaacttttc tttcatggtt tcctccacat aatcaatcat tgtattcatt
     6421 at
//
LOCUS       DAKTLH010000143         6381 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.145, whole genome shotgun sequence.
ACCESSION   DAKTLH010000143 DAKTLH010000000
VERSION     DAKTLH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.145"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(459..2831)
                     /locus_tag="O0394_002292"
     CDS             complement(459..2831)
                     /locus_tag="O0394_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCY0500357.1"
                     /translation="MAIKKKDRIIGVKELEIPQELKLVPNWVLWRAEWNEKQQNFGKV
                     PYSINGYRASTTNKKTWCDFESVSIEYEVDEQYSGIGFVLSDGNNFVCLDIDNAIDKK
                     GQINSELALKMMQLTYCEKSPSGTGLHCFFKGKLPDNRKKKRTDLDIELYDSARFMTV
                     TGCTIGQSDICDNQEVLNTLVDEYFKENLPANEVVREESNTNIQLSDEDIINIMMKSK
                     QKDKIKDLLQGTYESYFESSSEAVQSLLHYLAFYTGKNKQQMERIFLNYNNLTDKWES
                     KRGNTTWGQLELDKAIKNQKTIYTKSIDEFNVIPQGSKDVKQLLNQLGHEERTKMEEN
                     WIEEGKRGRKPTTISPIKCAYILNEHLTFILFDDEENTKLAMYQFDEGIYTQNTTIIK
                     RVISYLEPKHNSNKADEVIYHLTNMVDIKEKTNSPYLIPVKNGVFNRKTKQLESFTPD
                     YIFTSKIDTSYVRQDIVPEINGWNIDRWIEEIACNDNQVVKLLWQVINDSMNGNYTRK
                     KAIFFVGDGNNGKGTFQELLSNVIGYSNIASLKVNEFDERFKLSVLEGKTAVIGDDVP
                     VGVYVDDSSNFKSVVTGDPVLVEFKNKPLYRATFKCTVIQSTNGMPKFKDKTGGTLRR
                     LLIVPFNANFNGIKENFKIKEDYIKNQQVLEYVLYKAINLDFETFDIPDASKKMLEVF
                     KEDNDPVYGFKVNMFDQWTIRKVPKYIVYAFYKEYCDENGYNALSSNKFYKQFEHYLE
                     NYWKTDAQRRYDNEELAKRIYNFNDNRNYIEPIESGKNYKSYEKVKLKAI"
     gene            complement(2923..3171)
                     /locus_tag="O0394_002293"
     CDS             complement(2923..3171)
                     /locus_tag="O0394_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HCY0500358.1"
                     /translation="MNWEIKNLMCDLKILKEKFEDLKDNHGSHFEDLYLHEPNHTLNK
                     DDAIKEGFSYHERRIHNDQMFDALEKASSFADQSQDNA"
     gene            complement(3172..3555)
                     /locus_tag="O0394_002294"
     CDS             complement(3172..3555)
                     /locus_tag="O0394_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0500359.1"
                     /translation="MEIKQKYQLSKVVKILEVVLYEEDKFQSDKDYHYQDKALYEYAL
                     KLVHNGLFNILAELDFEDEAFLILDEVTMTLSDVMKETQHVYRYSVIDEKGEHKHTTD
                     RKGHVIGMLEWALDYIVGNIEVEEL"
     gene            complement(3557..3760)
                     /locus_tag="O0394_002295"
     CDS             complement(3557..3760)
                     /locus_tag="O0394_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0500360.1"
                     /translation="MRKQVIITKTVVGWYNIKDTQHNLMLNIPPKVFEQYFPDVSKDV
                     QVACLEMDLSKITEIKNKKKVGS"
     gene            complement(3727..3900)
                     /locus_tag="O0394_002296"
     CDS             complement(3727..3900)
                     /locus_tag="O0394_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0500361.1"
                     /translation="MKMYLTYICLVSLLTILLLAISNMYVAFSVYGMMVTYGFNLTGE
                     ITTCENKLLLQKQ"
     gene            complement(3912..4184)
                     /locus_tag="O0394_002297"
     CDS             complement(3912..4184)
                     /locus_tag="O0394_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCY0500362.1"
                     /translation="MSRTKLHDVPAKENTITEPKQVVVNPLFAKPNALASILGISYSS
                     VNRILKEWEKDSKGVDDLYYSLSSTMIVISIPRFEEYMKARHKKWM"
     gene            complement(4177..4440)
                     /locus_tag="O0394_002298"
     CDS             complement(4177..4440)
                     /locus_tag="O0394_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0500363.1"
                     /translation="MVLNLKRLRAERIACGITQDEMAHKMGWKTRTPYAKRENGIVDI
                     GANEFIKMAKILGYETNNLDIFFTNNVPGKERKNILKGGELNV"
     gene            4633..4965
                     /locus_tag="O0394_002299"
     CDS             4633..4965
                     /locus_tag="O0394_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0500364.1"
                     /translation="MRTNDEIITIIKTSMKEQNMSLSELARRVGVAKSAVSRYLNLTR
                     EFPLNRAEDFAKVLGIKTEYLLGFAEREESTKQDTIAAHLDGDFTEEELIEIRKYAEL
                     VRKAHRNQ"
     gene            4977..5435
                     /locus_tag="O0394_002300"
     CDS             4977..5435
                     /locus_tag="O0394_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCY0500365.1"
                     /translation="MYLYEKMVIENKEIPIDDGKSLGNFEGLYDNGVILINKNLSETR
                     KAEVLYEELAHHKLTYGNILDQSKWINRKFENYARRHGFISAVPLREIVEAYNYGVRN
                     LYELSEYLQLSEKYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            5452..6180
                     /gene="seq"
                     /locus_tag="O0394_002301"
     CDS             5452..6180
                     /gene="seq"
                     /locus_tag="O0394_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type Q"
                     /protein_id="HCY0500366.1"
                     /translation="MNKIFRVLTVSLFFFTFLIKNNLAYADVGVINLRNFYTNYQPET
                     LQGVSSGNFSTSHQLEYIDGKYTLYSQFHNEYEAKRLKDHKVDIFGISYSGLCNTKYM
                     YGGITLANQNLDKPRNIPINLWVNGKQNTISTDEVSTQKKEVTAQEIDIKLRKYLQNE
                     YNIYGFNKTKKGQEYGYQSKFNSGFNKGKITFHLNNETSFTYDLFYTGTGQAESFLKI
                     YNDNKTIDAENFHLDVEISYEKTE"
     gene            6204..>6381
                     /locus_tag="O0394_002302"
     CDS             6204..>6381
                     /locus_tag="O0394_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HCY0500367.1"
                     /translation="MKKLIVIILINIITLSVSNSASAQGDIGIDNLRNFYTKKDFVDL
                     KDVKDNDTPIANQLQ"
BASE COUNT         2062 a         1092 c          855 g         2372 t
ORIGIN      
        1 ttatagtaac tcagtaactt tttgtcaaaa agactaatat gaaagtaaaa actgtggtta
       61 ctgaattcta gaattttagt atcttttagt gacttaaacg ttttttagta actttagtat
      121 cttaggcaat tttgtataac tgcaagtctc ttccgattat gtgcaactga aatttacagc
      181 tatgcaactc gttttaaatt ttccagttgc aatttttttc ttactgtcac aaggggttaa
      241 tccacttttg caactatgca accgaaatca cacaaattag tttatataat ttttgatact
      301 atttcttatt tatattttgg ttatttactt ttctactaat aagttataga ttttgagttg
      361 ctcagttgca taagtgccct aaacccttga tataactgtg attataggtg caacccgttt
      421 taaaattttc ggttgcattc ggttgcattc ggttgcaact atattgcttt tagcttcacc
      481 ttttcatacg atttatagtt ttttccactt tcaataggtt caatgtaatt tctattgtca
      541 ttaaagttgt atatcctctt agcaagttct tcattgtcat atcttcgctg tgcatcagtt
      601 ttccaataat tctctaaata atgttcaaat tgtttataaa acttgtttga actcaatgca
      661 ttatagccat tttcatcaca atattcttta taaaatgcgt atacaatata tttcggcact
      721 tttctaatag tccattgatc aaacatattt actttaaacc cataaactgg atcgttatct
      781 tctttaaata cttcaagcat ttttttagat gcatcaggaa tgtcaaaggt ttcaaaatct
      841 aaattaattg ctttataaag cacatactct agcacttgct gattttttat atagtcttct
      901 ttgattttaa aattctcttt aatgccatta aaattggcat taaacggtac tatcaataac
      961 cttcttaaag tcccgcctgt tttgtcttta aatttaggca ttccattcgt tgattgaata
     1021 actgtacact taaaagtcgc tctatataag ggtttatttt taaactcaac caacactgga
     1081 tcgccagtaa ctacactttt aaagtttgaa gaatcatcga catacacacc aactggtaca
     1141 tcgtctccaa ttactgctgt cttgccctct aacacactca atttaaaacg ttcatcaaac
     1201 tcattcactt ttaagctagc aatattgcta taacctatta cattagacaa taattcttga
     1261 aacgtccctt taccattatt tccatcacca acaaagaaaa tagccttttt acgagtgtag
     1321 ttaccattca ttgagtcatt aattacttgc cacaataatt taacaacctg attatcatta
     1381 caagcaatct cttctatcca ccgatctata ttccagccat ttatttcagg tactatatct
     1441 tgccttacat acgatgtatc tattttactg gtaaatatat aatcaggtgt aaatgattct
     1501 agttgcttcg ttttacggtt aaatacacca tttttaactg gtattaagta tggtgagtta
     1561 gttttctctt ttatatctac catattggtt aaatgataaa taacttcatc agctttgttg
     1621 ctattatgtt tgggctctaa ataggaaatc actcgtttta taattgtagt gttctgtgta
     1681 tatatccctt catcaaattg atacatagct aacttagtat tttcttcatc atcaaaaagt
     1741 ataaatgtta aatgctcatt caaaatatat gcacatttta tagggctaat tgttgtaggc
     1801 tttcgccctc gttttccttc ttcaatccaa ttttcttcca tttttgttct ttcttcatgc
     1861 cccaattgat tcaacaactg tttaacatct ttactcccct gtggtataac attaaattca
     1921 tctatcgatt tagtataaat tgtcttttga ttctttatag ctttatctaa ctcaagctgt
     1981 ccccaagtcg tattacctcg tttactttcc catttatccg taagattatt gtagtttaaa
     2041 aatatacgct ccatttgctg tttgttttta cctgtgtaaa acgctaagta gtgtaataag
     2101 ctttgtactg cttcgcttga actctcaaaa tatgattcat atgtgccttg taatagatct
     2161 ttaattttat ctttttgttt agatttcatc ataatgttta taatatcttc atcagataat
     2221 tgtatattgg tattagattc ctctctcaca acctcatttg ctggcaaatt ctctttaaaa
     2281 tattcatcaa ctagagtatt taatacttct tgattatcac aaatatcact ttgaccaatt
     2341 gtgcatcctg taacagtcat aaaccttgct gaatcatata actctatgtc taaatctgtt
     2401 cttttctttt tacggttatc tggtagttta cctttaaaga aacaatgtaa tcctgtacca
     2461 cttggagact tttcacaata tgtgagttgc atcattttta gtgctaattc agaattgatt
     2521 tgtccttttt tatcaattgc attatcaata tctaggcaaa caaaattatt accatcactt
     2581 aatacaaaac ctataccgct atattgctca tcaacttcat attcaatact tacactttca
     2641 aagtcacacc atgttttttt attggttgta ctagctctgt aaccattaat actatatggt
     2701 acttttccaa aattttgttg cttctcgttc cattcagctc gccataatac ccaattgggc
     2761 actaatttta attcttgcgg tatttctaat tctttaactc caataattct atcttttttc
     2821 ttaattgcca cagaatgcct cctttctaat ctgatttctc tgtattttca atgcaactaa
     2881 tgttaaaata ttaatggtat gattttataa atttttattt ttttatgcgt tatcttggct
     2941 ttggtcggcg aaagatgacg ctttttctaa tgcatcaaac atttgatcat tgtgaatacg
     3001 tctctcatga tatgaaaatc cttctttaat agcatcgtct ttatttaagg tatgatttgg
     3061 ctcatgtaga tataaatctt caaaatgcga gccatgatta tcctttaaat cctcaaactt
     3121 ttctttcaat atctttaaat cacacattaa atttttaatt tcccaattca tttataattc
     3181 ctccacttca atatttccca caatgtaatc taatgcccac tctaacattc caatcacgtg
     3241 tcctttgcga tctgttgtat gtttatgttc acctttttcg tctatgacac tataacggta
     3301 aacgtgttgt gtttctttca tgacatcact tagcgtcatt gttacttcat caagaattaa
     3361 aaatgcttca tcttcaaaat ctaattcagc aagaatattg aacaatccat tatgaactaa
     3421 ctttaaagca tattcatata atgctttatc ctgataatga tagtccttat cggattgaaa
     3481 cttatcttcc tcgtataata ctacttctaa tattttaacc acttttgata attgatattt
     3541 ttgtttgatt tccatcttaa ctacctactt ttttcttatt tttaatttct gtaatttttg
     3601 ataaatccat ttctaaacac gcaacttgaa catctttact aacatcagga aagtactgtt
     3661 caaatacttt tggcggtata tttaacatta aattatgttg agtatcttta atgttgtacc
     3721 agccaactac tgtttttgta ataataactt gttttcgcac gttgtaatct ctcctgttaa
     3781 attaaatcca taagttacca tcatgccgta cacactaaaa gcgacataca tgttagatat
     3841 tgctagtaat aatattgtta acaatgaaac taagcagata taagttaagt acattttcat
     3901 tgccttgcct cctacatcca ttttttatga cgcgccttca tgtactcctc gaatcgcgga
     3961 atactgataa caatcattgt tgatgataac gagtaatata aatcatcaac acctttagaa
     4021 tctttttccc actcttttaa aatgcgattc accgaactat atgaaattcc caaaatacta
     4081 gctagtgcat taggtttcgc aaacaaagga ttcactacaa cttgctttgg ttctgtaatt
     4141 gtattttctt tagctggtac atcatgcaat tttgttctag acatttaatt cacctccttt
     4201 taagatgttt tttcgttctt ttccgggaac gttattggta aaaaaaatgt ctaggttatt
     4261 tgtttcataa cctaatattt ttgccatttt aataaattca ttcgctccaa tatctactat
     4321 tccattttct ctctttgcat aaggcgttct tgttttccac cccattttgt gagccatttc
     4381 atcttgcgta ataccacaag ctattctttc cgctctcaat ctttttaaat ttagtaccac
     4441 gtttacacct cctatcgttc tcgttcgaga actaattaca atttaccatt tacgttctcg
     4501 ttcgtcaaca atttttactt aaaaaaattc aaaaagtttt ttcttcctta tatattgtat
     4561 tcttttggga acggtgttat aatcaaatcg ttcacaaata agaacaaata ttcaattcag
     4621 gagatacaag aaatgagaac taatgatgaa ataatcacaa taattaaaac atcaatgaaa
     4681 gaacaaaata tgtcactaag tgaattagct cgtcgtgtag gtgtagcaaa atcagcagta
     4741 tcaagatatt taaatttaac tagagagttc ccattgaatc gtgctgaaga ttttgcgaaa
     4801 gtacttggaa taaaaacaga atatttatta ggatttgctg aacgcgaaga atctacaaaa
     4861 caagatacta tcgccgcgca cttagatgga gattttacag aggaagaatt aattgaaatt
     4921 agaaagtatg cggagttagt tagaaaagca catcgaaatc agtaaggggt tattgcatgt
     4981 atttatacga aaagatggtt attgaaaaca aagagatacc aattgatgat gggaaatctt
     5041 taggtaattt tgagggtctc tatgacaatg gggttatttt aattaataaa aatttgtcag
     5101 aaacacgtaa agcagaagtg ttatatgagg aacttgccca ccacaagttg acgtatggca
     5161 acattttaga ccaatcaaaa tggattaata gaaaatttga aaattatgca cgtagacacg
     5221 gctttatctc agctgttcca ttacgtgaaa ttgtagaagc ttataattat ggcgtacgta
     5281 acttgtatga gttgtccgag tacctacaac tgagtgaaaa atacatatta gaagcgatag
     5341 aacaatataa aaagatatac ggtattggta cccactacgg cgagtattca attacatttg
     5401 agccgttgag agtttttaaa tataaagaaa tataacaaag gagaaatgaa aatgaataaa
     5461 atatttagag tactcactgt tagcttgttt ttcttcacat ttttaataaa aaacaatcta
     5521 gcatatgctg atgtaggggt aatcaacctt agaaactttt atactaatta tcaaccagaa
     5581 acgcttcaag gagttagttc tggaaatttt tctacttctc atcaattaga gtatattgat
     5641 ggaaaataca ctttatattc acagtttcat aatgaatatg aagcgaagag attaaaagat
     5701 cataaagtag atatctttgg aataagttac tcaggtcttt gtaatacaaa atatatgtat
     5761 ggcggaatta cgttggcgaa tcaaaattta gataaaccta gaaatatacc tattaatctc
     5821 tgggtcaatg gtaagcaaaa cactatatct acagacgagg tttctactca aaaaaaagag
     5881 gtaactgctc aagagattga tattaagtta cgaaaatacc tacaaaacga atacaatata
     5941 tatggtttta ataaaacaaa aaaaggtcag gaatatggat atcagtcaaa atttaattct
     6001 ggatttaaca aaggaaaaat tactttccat ttaaataatg aaacttcttt tacatacgat
     6061 ttgttttaca ccggaactgg tcaagcagag agttttttaa aaatttacaa tgataataaa
     6121 actatagatg cagagaattt tcatttggat gtagagattt catatgagaa gactgaataa
     6181 atagaattta aggagaaaat gcaatgaaaa aattaatagt tatcatatta ataaatataa
     6241 taactttaag tgtctctaat agtgccagcg ctcaaggtga tataggaatt gataatctca
     6301 ggaattttta tacaaaaaaa gactttgtag atttaaaaga tgtaaaagac aatgatactc
     6361 ctatagctaa tcaactacaa t
//
LOCUS       DAKTLH010000144         6298 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.146, whole genome shotgun sequence.
ACCESSION   DAKTLH010000144 DAKTLH010000000
VERSION     DAKTLH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.146"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(113..961)
                     /locus_tag="O0394_002303"
     CDS             complement(113..961)
                     /locus_tag="O0394_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0500368.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
     gene            complement(1031..1447)
                     /locus_tag="O0394_002304"
     CDS             complement(1031..1447)
                     /locus_tag="O0394_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0500369.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     VRDLLQYKIDNYDNHIAKINASDNYDDNVERLWERK"
     gene            1691..1858
                     /locus_tag="O0394_002305"
     CDS             1691..1858
                     /locus_tag="O0394_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500370.1"
                     /translation="MRKIWVIDCLYASTFFVDCLTVDSHLYTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
     gene            1855..4278
                     /gene="hysA"
                     /locus_tag="O0394_002306"
     CDS             1855..4278
                     /gene="hysA"
                     /locus_tag="O0394_002306"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase HysA"
                     /protein_id="HCY0500371.1"
                     /translation="MTYRIKKWQKLSTITLLMAGVITLNGGEFRSVDKHQIAVADTNV
                     QTPDYEKLRNTWLDVNYGYDKYDENNPDMKKKFDATEKEATNLLKEMKTESGRKYLWS
                     GAETLETNSSHMTRTYRNIEKIAEAMRNPKTTLNTDENKKKVKDALEWLHKNAYGKEP
                     DKKVKELSENFTKTTGKNTNLNWWDYEIGTPKSLTNTLILLNDQFSNEEKKKFTAPIK
                     TFAPDSDKILSSVGKAELAKGGNLVDISKVKLLECIIEEDKDMMKKSIDSFNKVFTYV
                     QDSATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNDT
                     TLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDAMDDSTK
                     AKYKKIVKSSVESDSSYKQNDYLNSYSDIDKMKSLMTDNSISKNGLTQQLKIYNDMDR
                     VTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYHDNFWV
                     TADMKRLSGTTTLDNEILKDTDDKKSSKTFVGGTKVDDQHASIGMDFENQDKTLTAKK
                     SYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTNSDNQENNSVF
                     LESTDTKKNIGYHFLNKPKITVKKESHTGKWKEINKSQKDTQKTDEYYEVTQKHSNSD
                     NKYGYVLYPGLSKDVFKTKKDEVTVVKQEDDFHVVKDNESVWAGVNYSNSTQTFDINN
                     TKVEVKAKGMFILKKKDDNTYECSFYNPESTNSASDIESKISMTGYSITNKNTSTSNE
                     SGVHFELTK"
     gene            complement(4338..5192)
                     /locus_tag="O0394_002307"
     CDS             complement(4338..5192)
                     /locus_tag="O0394_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCY0500372.1"
                     /translation="MNKTNFGAYLNKSKSRKIYKIFDQNLKREFSYWQFRKLFKSYTR
                     QSDVHELKVCTQNDGTIEQIWIDEANQFGVSVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEIFWGGDNELYNYHYLYPHQRYAIDLVIKENHKTYKNQGKVNTDYFC
                     FGKDIIAPANGTVEKVVNGVQDNSIGSTNESQFLGNYIVIKHAENEYSLIAHLHQYSI
                     IVNEGQNVKYGDIIGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFINNRELIKGD
                     VVCGLQAE"
     gene            complement(5403..6218)
                     /locus_tag="O0394_002308"
     CDS             complement(5403..6218)
                     /locus_tag="O0394_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HCY0500373.1"
                     /translation="MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVI
                     TAVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEP
                     FLFEDIEDILRHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDSRILEALVRYA
                     NDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMRKYNIPVKSILGFGDS
                     GNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY"
BASE COUNT         2153 a         1048 c          929 g         2168 t
ORIGIN      
        1 tatttgcttg ctcctttcac tttttaagag taaaaaataa cctgaaacat agactttaac
       61 atccaagttt caggttagtg taatgtaaat gatttacttt gttatattat gattaaacat
      121 ctaattctct gcttgtaagt gctttaatca tctctggatc agcatgtgag aaaaattggc
      181 tattactttc ttctaatgtc gcaatttgtt gtttatcttc ttctgtaagt tcaaaatcaa
      241 atatatctaa gttttgagcc atacgttctg gattcactga ttttgcgagt acaacaatgt
      301 cacgttctac taaccagcgt aaaataactt gtgcaattga tttgttgtat ttatctgcaa
      361 tggcctgcaa caattgattt tggaataatt gatttttacc ttctgcaaat ggtgcccaag
      421 cttctaccac aacattttct tgttgaagtg ctgcaacttg ttcttcttgt tgatggaatg
      481 gattaatttc tatttggttc acttgtggtt gaatttgatt gtgaatacct aaatctacaa
      541 ttcgatcaac gccgaagtta gatacaccga ttgctttaat tttaccattt tcttttaact
      601 cttctaatgc acgccatgag ccatatacgt cattgtaagg ttggtgaata agcactagat
      661 caagatagtc caaatttaat cgttgtaatg atcgttcgat tgatttgata gtatcttcat
      721 agttaacatt ttcaatccat actttagtcg taatgaatag ttcacttcta tcaatacctg
      781 aagcttcaat accttgacct acttcagttt catttaaata actttgtgct gtatcaatat
      841 gtctataacc cgctttaatt gcttctttaa ccgcttctgc tgtttgttct tgaggaattt
      901 gaaatactcc gaaacctaat actggtatct ttacatcttt acttatttca atatgattca
      961 taaaatgaac ctcctttatc ttgtatgtcc actataaacc atggagcgaa ctctatagca
     1021 agccataggt ttactttctc tcccaaagac gttcaacatt gtcatcataa ttatcactag
     1081 cattgatttt agcaatatga ttatcataat tatcaatttt atattgtagt aaatctctca
     1141 catcatgaat tgtctttaag ttttcattta attcttcgag ttgcttattt aaaatttgct
     1201 tttgctgtgc ttgaatattt tcatttttag gaagctgcgc taacctgcaa aattcgataa
     1261 gcgattcaat actgacgcct gcatttcgca aatttttcac taaataaatc caatttaaat
     1321 cactatcatt atatattcta tatccatttt catctcgatt aactggtgga atcacaccaa
     1381 ccttttcata atatcttaaa gtgtcttgag atatattcat gagcgctacg acttctttag
     1441 ttttcattgc gactatcctt tcagttatgt ttggtcgtct aaagtaatgt tgctttatat
     1501 attgtcatct tcgtttgaat acttcttatt ttattactca aatttaaatt tgtctctttt
     1561 ttaacatttt acatttcatc gtttttaatt actttaaaaa ttgtataact taaatattta
     1621 aaatgatata atcactaaga ttgataatat ttaatttttt gaaaattatt ttagttgcaa
     1681 tttttgggtg atgcggaaaa tttgggtgat cgattgttta tatgcatcaa cattctttgt
     1741 ggattgtttg acagtagaca gtcatttgta tacgttaacg aattttgaaa aattgaatca
     1801 ttatttaatc ataatattca ttgcgcacgc gcaacacaaa aaggagacat gtcaatgaca
     1861 tatagaataa agaaatggca aaaattatcc accattacgt tattaatggc tggtgtgatt
     1921 actttgaatg gtggtgaatt cagaagtgtt gataaacatc aaatcgctgt ggctgatacg
     1981 aatgttcaaa cgccagatta tgaaaaattg aggaacacat ggctggacgt taactatggt
     2041 tatgataagt atgatgagaa taatccagat atgaagaaga agtttgatgc tacagagaaa
     2101 gaggcgacga atttactcaa ggaaatgaaa actgaaagtg gtaggaaata cttgtggtca
     2161 ggagcggaaa cccttgaaac taattcttct catatgactc gtacctatcg taatattgag
     2221 aaaatcgcag aagcgatgag gaatcctaaa accactttaa atactgacga aaataagaag
     2281 aaagtgaaag atgcccttga gtggctgcat aaaaatgcat atggaaaaga accagataaa
     2341 aaagtaaaag aattaagtga gaattttact aaaacaactg gcaagaatac caacttaaat
     2401 tggtgggatt atgaaattgg aacacctaaa tcattaacaa atacgcttat attgctgaat
     2461 gatcaatttt caaatgaaga aaagaaaaaa ttcactgccc ctattaaaac tttcgcccca
     2521 gatagtgaca aaatattatc ttctgtagga aaagctgaac ttgctaaagg cggaaatcta
     2581 gtagacattt ctaaggtgaa acttttagaa tgtattatcg aagaagataa agatatgatg
     2641 aaaaagtcta tagattcatt taataaagtc ttcacttacg ttcaagattc tgccactggt
     2701 aaagaacgta atggatttta taaagatggc tcttacattg atcatcaaga cgttccatac
     2761 actggtgctt atggcgttgt actcttagag gggatttctc aaatgatgcc gatgataaaa
     2821 gaaacacctt ttaatgataa aacccaaaat gatacaacct taaagtcatg gattgacgac
     2881 ggatttatgc cactcattta taaaggtgaa atgatggatt tatcacgagg tagagctatc
     2941 agtcgtgaaa atgaaaccag tcactcagca tctgcaacag taatgaaatc attgttgaga
     3001 ttaagtgatg ccatggatga ttcaacaaaa gctaagtata aaaagattgt caaatcttca
     3061 gtagagtcag attcaagtta taaacaaaat gattatttaa attcatattc ggacatagat
     3121 aaaatgaagt ctttaatgac agataacagt atttctaaaa acggattaac acaacaactt
     3181 aaaatatata atgacatgga tcgtgtcacc tatcataaca aagacttaga ctttgcattt
     3241 ggtttaagta tgacgtcgaa aaacgtagca cgctatgaaa gtatcaacgg agagaattta
     3301 aaaggttggc acactggtgc tggaatgtct tatttatata acagcgatgt caaacactat
     3361 catgataact tctgggtgac agccgatatg aaacgtttat caggtacaac aactttagac
     3421 aatgaaatat taaaagatac ggatgataaa aagtcgagta aaacttttgt tggcggaaca
     3481 aaagttgatg accaacatgc tagtatcgga atggattttg aaaatcagga caaaacttta
     3541 actgccaaaa aatcatattt catattaaac gataaaattg tcttcttagg aactggcatt
     3601 aaaagtactg attcatcaaa gaatccagtt acaacgattg aaaatcgcaa agcgaatggg
     3661 tatacgttat atacagacga taaacaaaca accaattctg ataatcagga aaacaattca
     3721 gtctttttag agtccacaga taccaaaaag aacatcggtt atcatttttt aaacaaaccg
     3781 aaaataactg taaaaaaaga aagtcatact ggtaagtgga aagaaataaa taaaagtcaa
     3841 aaggatacac aaaaaactga tgagtattat gaagtaactc aaaagcattc taattctgac
     3901 aataaatatg gatatgtgtt gtatccaggc ttatctaaag atgtctttaa gacaaaaaaa
     3961 gatgaagtaa ctgtcgttaa gcaagaagat gacttccacg ttgtgaaaga taatgaatcg
     4021 gtttgggctg gtgttaatta tagtaatagc actcaaactt ttgacattaa caacactaaa
     4081 gtcgaagtta aagccaaagg tatgtttata cttaaaaaga aagatgataa cacttatgaa
     4141 tgtagcttct ataatcctga atctacaaat tccgcttcag atattgaatc taaaatttca
     4201 atgaccggtt actctattac aaacaaaaat acgtcgactt ctaatgaatc cggcgtgcac
     4261 tttgaattaa ctaaataagc aagaaaatct cgacactata ccatttttat agtgtcgaga
     4321 ttttataagt atcgccatca ttcagcttgt aatccgcaga ccacatcccc tttgataagt
     4381 tctcgattat ttataaatcg aatttttaaa gatgtacatg cttcaatatt cttatcattc
     4441 attacttgaa aatgtatatg aggttcagta gaattgccag aattcccaac cttcccaatg
     4501 atatcaccat atttaacatt ttgcccctca ttcacaatga ttgaatattg gtgtaaatga
     4561 gctattaagc tatactcatt ttctgcgtgc ttaattacaa tataatttcc taaaaactgt
     4621 gattcattcg tacttccaat actgttgtct tgaaccccat tcactacttt ttcaactgta
     4681 ccatttgctg gcgcaatgat atcttttcca aaacaaaaat aatcagtatt tacttttcct
     4741 tgattcttat atgttttatg attttcttta ataactaaat caatagcata cctttgatgt
     4801 gggtataaat agtgataatt atataattca ttatctccac cccaaaaaat ctcatagcaa
     4861 tctgttgtcg gcactgcgta ttttaaagtc gtttcattct taatcgcatc ataattaatt
     4921 aacggacgca taacaaatcc gataatacta ttatctttcc aagtcactga cactccaaat
     4981 tgatttgctt catctatcca tatttgctca atagtaccat cattttgagt acacactttt
     5041 aattcatgta catcagactg ccttgtataa gatttgaaaa gtttcctaaa ctgccaataa
     5101 ctaaactctc gcttcaaatt ttgatcaaaa atcttatata ttttcctgct cttactttta
     5161 tttaaataag ccccaaagtt tgttttattc atatatactt aacctattat attaattttg
     5221 gtatcaattc tttctccagt tcctgcgtca tttttgcgct acctgaatcg tctacactta
     5281 attggataaa tcaacatata aataattttt taaagctaaa gtgtagtcga ttcaccatat
     5341 tttctaatca tcccttaaat attaatattt gcacttaaaa agtatacata tcactcaact
     5401 aatcaataat catacttacc ttcaacaaat tctttaacat gcagtgttat cccttgataa
     5461 tatggatatt ttgttaatct aaatttctgc tttaatgctt catctctact attcgacatc
     5521 aaatatgcat gttcaagata gcttaaatat gcttcgtcat tgccgctatc tccaaaacct
     5581 aatattgatt ttactggtat attatatttc ctcatcagaa attgagtagc atataatttt
     5641 ccagcatttg acggtgtgaa gtcaatatca tacgcatttt caggatcacc agctaatgga
     5701 ttgcttcgac taaatctcgc tgtatagttt tggtcatttg cgtatctgac aagtgcttct
     5761 aggatacgac tatcattgtt gtgattacct gttgaatgaa aataaaaatt ataatgtgtt
     5821 tcattacctt caaatgctct ttgtggaata aattcgacat tatattgcgc agaaatatga
     5881 cgtaatatat cttcaatatc ttcaaataaa aatggttcgc tcaagacttt ctttttatat
     5941 gtttctgatt caacatattc gccattattc caagtgaaca ttttagaact caaatcagaa
     6001 aagatatgta atggtttaaa cgtcatatta acttgatcca ttttatccat gacactttga
     6061 aatgtacttc ctgtcaatac agcagtgatc acttcattat tattagttag tttttccaac
     6121 aatttttcca tttctcttaa atgacttaaa tcttcttcat ttgtattatg tttaaaatat
     6181 gtttcatcaa aatcaaacaa taatagacgc tttgacatac tcattccccc ataatatttt
     6241 aatgaattta tctataactt aaatacacta taagtctcaa ttaaaagtat aagatcga
//
LOCUS       DAKTLH010000145         6142 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.147, whole genome shotgun sequence.
ACCESSION   DAKTLH010000145 DAKTLH010000000
VERSION     DAKTLH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.147"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            186..950
                     /locus_tag="O0394_002309"
     CDS             186..950
                     /locus_tag="O0394_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCY0500374.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
     gene            963..1415
                     /locus_tag="O0394_002310"
     CDS             963..1415
                     /locus_tag="O0394_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCY0500375.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(1557..3041)
                     /locus_tag="O0394_002311"
     CDS             complement(1557..3041)
                     /locus_tag="O0394_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCY0500376.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(3162..3530)
                     /locus_tag="O0394_002312"
     CDS             complement(3162..3530)
                     /locus_tag="O0394_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCY0500377.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            complement(3663..4043)
                     /locus_tag="O0394_002313"
     CDS             complement(3663..4043)
                     /locus_tag="O0394_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HCY0500378.1"
                     /translation="MLNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTVAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            complement(4044..4700)
                     /locus_tag="O0394_002314"
     CDS             complement(4044..4700)
                     /locus_tag="O0394_002314"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCY0500379.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA
                     "
     gene            4884..5714
                     /gene="thiD"
                     /locus_tag="O0394_002315"
     CDS             4884..5714
                     /gene="thiD"
                     /locus_tag="O0394_002315"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCY0500380.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
BASE COUNT         2023 a         1101 c          991 g         2027 t
ORIGIN      
        1 caataatttt tcctttttat acttcaagag aattttaact actaaaaatt ccgatgatta
       61 ttattacaat agtatcaaat attagttttt taaaatcaat aacaacttat caaaaagctc
      121 atgtggttat tttatagtgt ataaactata atgagtatta aattcttata aacaatggtg
      181 atgaaatgga cataaattca gaagaataca aacaagaggt acttatcaaa gacgttgtca
      241 tgcttgctgc tcgcatacta ttagaatctg gtgcagaagg tacgcgtgta gaagatacca
      301 tgacacgtat tgcaaaaaaa cttggttaca gtgaaagtaa cagctttgtt acaaacactg
      361 tcatccagtt tacgttacat tcggaatcgt ttcctagaat atttagaatt acctctcgag
      421 atacaaactt aataaaaatt tctcaagcta ataaaatttc gcgtcaaatt acaaacaatg
      481 aaatttcttt agccgaagca aaaacgcaac ttgaaaaaat atatgttgct aagcgtgaca
      541 gcagtcttcc ctttaaaggt tttgctgcag caatgattgc aatgagtttc ttatatttac
      601 aaggtggtag attgattgat gttttaactg cgatattagc aggtagtcta ggatacctag
      661 tcactgagat tttagatcgt aagttacacg cacagtttat cccagaattc attggttcat
      721 tagttattgg gattatcgcc gttattggac atacacttat tccaacaggt gacttggcaa
      781 ctattatcat tgcggcagtc atgcctattg ttcctggtgt attaataaca aacgcaatac
      841 aagatttatt tggtggacac atgttgatgt tcacaacgaa atcattagaa gcattggtta
      901 ctgcgtttgg catcggtgct ggcgttggta gcgtattaat tttagtatag gagtatcaga
      961 ctatgttttg gatcttaaac tttatcttta gctttttagc ttcaatgttc ttctgcgtca
     1021 tttttgatgc acctagaaaa ttatatctat catgtggttt cgttggtacg tgtggatgga
     1081 tggtttacac cttattcttc aacggcttta atgtgcacac tatatactct agtttctttg
     1141 gtagtttagc attaggcttg ttaagtcatt atatggctcg taaacaaaaa gaacctgcca
     1201 tcatttttat ggtaacgggt atcattccat tagtacctgg tggcttagca tacgatgcta
     1261 caaaaaattt agtcttatta aatttcagta cagcaatcaa taccatgcta gaggttacac
     1321 ttattgcagg cgccatcgca ttaggtttat tattcgccga ccaaatttcc aaattaattg
     1381 tttctgggtt cgtgaaatct tttaaacgat tataaatatt tgttagaaat ggctcagccc
     1441 taactcttag caattatata taaggttcag ctgcctcgtc ttaaatgtca atttataata
     1501 caaaaaatcg cacgatacgc ctccgaatga agaaggttta ttgtgcgatt cttttattat
     1561 ttagtttttt ctttttcagg ttcttcaatc atatcatcaa cttcattttc acttctattt
     1621 aaaatcaagc gacttaattc atcattatcc atatttttca atttaggata tcgaatcaca
     1681 aagaatatta aaccgatgat taaccatcca agtaatgcaa tataagacgg tgcagtcagt
     1741 gctgcaggag aacctggcac taataacaac gctaagaaaa tgaatgatac aaatgagccg
     1801 ataatagcaa acgttttgta aaccggtgca tacgtattac tttgtttgtt ataactgaat
     1861 aatttcgctg cagacaaaca tgtaataaag taggcaatgg atacaccagt agatgacata
     1921 tctacaatcc aagtcaatgc agttcttcct agccaaggtg caattaacga cactcctact
     1981 aggaatatga ttgcgacata tggtgttttg tatttactat gtaatttact aaacattgtt
     2041 ggcataatac ctgaacgtcc catagaaaat aacaagcgac ttgaactcat caagaatcca
     2101 tttaaaccag taaatatacc catcataatt gcaattgcta atacacctaa tccaatataa
     2161 ccaaatgctg tttgtgtaac agcacctgtt aaccacaact gcccatttaa actttgatga
     2221 cttgttgata accaaccagt gtataaaatc atgacaacat aagttaatga tgctgctaat
     2281 aaactgtaca cgataagctt aaatgtcttg tttggtgcaa agttaaactc ttctgctgtt
     2341 tgtggaatat tatcaaatcc aacatatgcc catggtgcca cggatacaat aaccacaata
     2401 gacactaacc atcctttgct aggttcagct aacggttgta aattttcaag tgcaaaatta
     2461 ttaccaaaga atgaaccaaa gaacatcaat aatacgacga ttaccatcgc cacacagaaa
     2521 taatattgta atgatccaga tacacttgcg ccacgaatcg ttactagcat gaatacaagt
     2581 agtaatacgg tcgcaataat gatttccgta atataaacgt cccagcccgc aatggtgtat
     2641 agtttcccat tatttaagac atctggcaat aagaatttaa ctagtaaact gaatgcggtc
     2701 gcatttaaag caacgacaca gacataacca aaagttaaaa accatgatga gaagaaactc
     2761 acatatctgc cgaaacttaa gaaactaaag gcaaacgcgc cccctgatac tggaaatctc
     2821 tctactaatg cgccataact aaccgcaatt aatatcatta ataatgcacc aataactata
     2881 ccaattgatg ctgcaatcgg acctgactgc ttaatccagt ctcctggtaa gatgaatgcg
     2941 ccccatccga tacatgaacc atatgcaatc gcccatacaa acttttcaga taggttttgt
     3001 tttaaatcgc ctctatctat ttgcttattc tttttttcca taaataaaaa ctcacctcga
     3061 aggtattcta tacccaaaag atgagtttgt aactcttttt cttgattatt tttattaaaa
     3121 atttaatgtt tagttacata ggtaatctaa agttttaaaa tttaaccagc aaatttgaat
     3181 gtcgcaatga ttaacattat ccatccaatg atgaacaata cgccaccaat tggcgtaatc
     3241 gcacctaaaa ctttaatttg agttaatact aaaatatata atgatccact aaagaaaata
     3301 ataccagcaa atattaacca gccagcccag ttaacattga ttgaagttgt accactaatt
     3361 acacctataa ttaataatgc taagccatgg tacatttgat acgtcgttgc tttttcccat
     3421 actgataaat agtgatcact tatttttcct tgtaaaccat gcgcaccaaa tgcacctgta
     3481 ccgacagcca tcatcgcgtt taatgcacct aaaataataa ataatttcat tgctacctct
     3541 ccttatataa ttcagtatta ataaatgttt aaatccgaac actcgaataa aagtatatgc
     3601 gtcttgatta ttaacctaca tacttaacaa gctattaagt aattatgttt cattgttgct
     3661 ttttaaaaat caaatatgga ttcaccattg ccaatgtcat catctgtcgc taatctttgt
     3721 tgttggtttg aaggttgtga atatgactta ttatgatgat gtgctgaatg cgtatttact
     3781 ttaccaccca tcgcttcaat ttcagcaact gtcacttgat gagttggctg cgttatttgt
     3841 gattgtggca tttgattatg gttagcaata cgacgattca tttgttgttc accaatatgt
     3901 ggtgtatttg atgttgaagc atataaagat gttaatatat gtatggcata catatgtttt
     3961 tcaaagtcat ggtcatgctg tgcctgctct gcttgtacta attcttgctc aattcgtgct
     4021 attaaagttt ctctattcaa catctacgcc tcactttcac accaattaat tggtgatttt
     4081 cctactgact ctaaataggc attcgctttg gaatacggtt ttgatccaaa gaatcctcta
     4141 tatgcagaca gtggactagg atgcactgat tttataatac aatgtttaga tgtatcgata
     4201 agctttattt tttgctgtgc aggtttcccc cacaaaataa agacaacatg ttctttataa
     4261 tcagacactg ctttaataat ttcatcagta aatgtttccc aaccaatatc acgatgagaa
     4321 tttgcttcac cctgtcttac ggttaaaact gtattcaata acaagacgcc ttctcttgcc
     4381 caatcttgta aatgcggtgt ttgtctaacg catccaatat catctgctaa ttctttatac
     4441 atattacgta aagatggagg gaattttgcg ttaggttgca ctgaaaatgc taatccatgt
     4501 gcttggtttg gaccatgata cgggtcttgt cctaatataa caactttgat attttcaaac
     4561 ggtgttaaat caaacgcttg atatatattt tccctatcag ggtatacgat tgcagtcgaa
     4621 tattcttttt ctaaaaaatc atgcatagct ttaaagtcat gtttcgttgt tatgtcatga
     4681 aaaatttgcg accattccat tcaatctcac ctcaggacat attttaacat atcacactta
     4741 tctaaagtga atgacatttt caaaagttac aatagtatga atgattcatt tcaacaatca
     4801 tcaaatattg aatgcttaat atttttcaat taggttaaaa atgatatgat gaatacaata
     4861 ttattatgta ggagtgaagc tcaatggctt taaagaaagt tttaacaatt gccggttctg
     4921 acacaagtgc tggcgcaggt atgcaagcag atttgaaaac gttccaagaa ttagatacgt
     4981 atggcatggt cgctttaact gccatcgtta ctatggataa agatacatgg tcacacgatg
     5041 ttacaccatt accaatggat gtatttgaaa aacaacttga aacggcttta tcaatcggac
     5101 ctgatgctat taaaacaggt atgttaggta ctgaggagat aatcaaacgt gctggagaag
     5161 tatatgaggc atctaatgca caatattttg tagtagatcc tgtcatggtt tgtaaaggcg
     5221 aagatgaagt gcttaatcct ggaaatactg aggcgatgat taaatattta ctacctaaag
     5281 caacggtagt gacaccaaac ttatttgaag cgggtcaatt atcaggatta ggtaaattaa
     5341 attcaattga agatatgaaa aaggctgcaa caattatctt tgataaaggc gcacagcatg
     5401 tcatcattaa aggtggcaaa gctttagatc aagataaatc gtatgactta tactatgatg
     5461 gtcaaacatt ttatcaacta acaacagaca tgttccaaca aagttataac catggtgctg
     5521 gttgtacatt tgctgcggca acaactgcat atttagctaa cggtaagtca ccgaaagaag
     5581 ctgtgattag cgcgaaagct ttcgtcgctt ctgctattaa aaacggttgg aaaatgaatg
     5641 attttgttgg tcctgtggat cacggtgcat acaaccgtat tgaacatatc gatgttgaag
     5701 taacagaggt ttaacgatta ggcggttcat ctataaatca acattattag ccacaccact
     5761 taaattgggt atgaactcaa tttatgtgat gtggttattt ttttataatc atcatgctct
     5821 gtatataatt taaatttgaa agttacatat aagcgttatt agcttttatt gaaaatttca
     5881 tacatatcga acaatacata caaatatttc ctatacatct acatctatga ctaaataacc
     5941 ctttattttc aagatgaact taaactcatc tatatgctca atacctctat cattatcatt
     6001 cttttgcgat agatgcctct ctaatcatat atgttatatt tcccaaatga ctgccaggcg
     6061 actctataaa tatatattta tttagaactt ttatacataa atagtcttag taaatcatac
     6121 gttctatgtc tgataaaaag tt
//
LOCUS       DAKTLH010000146         6135 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.148, whole genome shotgun sequence.
ACCESSION   DAKTLH010000146 DAKTLH010000000
VERSION     DAKTLH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.148"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(31..1659)
                     /locus_tag="O0394_002316"
     CDS             complement(31..1659)
                     /locus_tag="O0394_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0500381.1"
                     /translation="MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFE
                     VKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVMVYKTNRLARNTSDLLKIVEDLH
                     KQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
                     LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPF
                     SISSITYILANPFYIGKIQFAKYKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQ
                     MRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNF
                     RNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDI
                     AYKQQQYDEVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNE
                     DKTLFDAKEISKLLQHIFHDIKHIEKSRLKALYLSVIDRIDIKKDGNHKKQFYVTLKL
                     NNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI"
     gene            complement(1681..3030)
                     /locus_tag="O0394_002317"
     CDS             complement(1681..3030)
                     /locus_tag="O0394_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0500382.1"
                     /translation="MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQ
                     SGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRNLKNALKLIKLCQTYHVHILSVH
                     DGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
                     NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYC
                     GRVNNQYGQFDNMFPSIVSTSIYEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLT
                     NMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEVCRDFYQNQRIYTKI
                     KSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSING
                     HQIQHIIQNIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA"
     gene            complement(3264..5051)
                     /locus_tag="O0394_002318"
     CDS             complement(3264..5051)
                     /locus_tag="O0394_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCY0500383.1"
                     /translation="MSYTLFEIGPFALDSFGWKETIPPKKDGDSEKVVRMASPIIVNA
                     RFSDPITGVEKLIITNNNGKKDIFESDILTTRNLPSLIKYGYSINEKYIRSLSYALQL
                     MRDRLPLSELYEGVGILETPFGYLISLDKVLKSIQFNQSSPSYPIVDSAYDLTPKGTF
                     DNWFNMYIDEVKGHLLLELAVIFGISALVTSFLKHKHEIEFAGILFSFTGQSSTGKST
                     AAALAVSVAGNPTKGNETLFRSWNATRNALEGYLSNNYGIPIVFDELSSTTLRDTTGL
                     LYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFNDSAQNDGLRVRTIEINEQF
                     TTNATNADNIKKAVALNYGHVLPLVAKYLINREDEVIQWFYKEVDWFEAKLKDETNNT
                     GIRMFKRYAVITTSAKILGRVLSTDIDIANIRDYFIDYHTHTVSERSLADKAIDVIIQ
                     FVAQNRGKFSDEGALKNMFENYGLISLKDDHIEVKIIANVFKQMLNNHQFQDVNNVVN
                     ALRDKGFILADRGRQTTKRSVKDNSGKKQSLVFYHLKLDVEFASILGLTKDKSLLQNW
                     TPSNDNKAAKELFKSANEGIGPSGVHEDF"
     gene            complement(5051..5341)
                     /locus_tag="O0394_002319"
     CDS             complement(5051..5341)
                     /locus_tag="O0394_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500384.1"
                     /translation="MNKKSKQQEKLYNFIIAKSFQQPVGSTFTYGELRKKYNVVCSTN
                     DQREVGRRFAYWIKYTPGLPFKIVGTKNGSLLYQKIGINPCNNSTPSKGGDC"
     gene            5480..>6135
                     /locus_tag="O0394_002320"
     CDS             5480..>6135
                     /locus_tag="O0394_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP synthase"
                     /protein_id="HCY0500385.1"
                     /translation="MIPTLEEIIDKYGNLVDYLKFDVTVEVYEDLLLLRSLSDLNEHQ
                     KMDRVSFIKKHLNSNFDYLLDDTEFRSINKIYSNLNIMTHINSQNNNFKRNPFINEEQ
                     LKSLLAIKKVDAHMSYDSNITSKALAEINKTQKRLVTKIDTLYNQSKKENEYVRLGEK
                     ISYTKLENHWKYLYNEIQFYNLNNQLISYVALEQEYAWAFLNELFYLIELYLKAFKGQ
                     "
BASE COUNT         1833 a          985 c         1063 g         2254 t
ORIGIN      
        1 aaattattgt tataacaatg attctattag ctaaatagta agatatagtg tttggggcaa
       61 aaataaagac gaagtgctga gatgcacttc gtcgagttgt ttattattga aaagttgttt
      121 aatgatttcg ttattaagtt tgagtgtgac atagaattgt tttttatgat taccatcttt
      181 tttaatatca atgcgatcaa tcactgatag atacaatgct ttgagtcgag atttttctat
      241 gtgcttaata tcatgaaaga tgtgttgtaa tagtttactg atttctttgg catcaaataa
      301 agtcttatct tcattttgtt gatttttgag ttggttgatt tgattcgtaa tgtcattgag
      361 ttgcttttca tatttttgaa tacttggtct gattactgat gttaagtccg gattatcctc
      421 gatggtttta atcaagttat ttattttgat ttgtacttca tcatattgtt gttgcttata
      481 agcaatatcg tgatgaagtg cagcgccatc aacttgattt tcttgattga cgtgtgttac
      541 tacgcgttga atgactttat cacttttgac tatttcaagt atttgcttca tcacataatc
      601 ttcaatcaca tcagctctta cactgtttgc cgaacatact ttggaaccct tgttccgaaa
      661 attactacat gaatagtaac gaatacgttt cttagtcccg tctttaagag tattcgtggt
      721 attgcttgct gccataggtg cgccacattg gggacagtga ataatgcctg taagcagatt
      781 cgttcctttg ccatggactt ggggtttttg actgacttgt ttcttacgca tttgtacttt
      841 atcccataaa tcttgattaa taatggggga atgcttacct tcagctatca ctggtttatc
      901 attcagccct ttacgacgtt tttcactcca atctttgtat ttcgcaaatt gaattttgcc
      961 gatatagaaa gggttagcta agatgtatgt gattgaacta atactgaaag gtttcccctt
     1021 tttagtgaca tatcctttgt gattcaatgc attggcaatt ttacgatagc catgtccttt
     1081 ggcatagcac tcgaatatat attttacaat attagcttca tgttggttaa tcattagctc
     1141 gtgtttacta tctggtattt tgtcataacc tagtggtaaa ttgccttgat aatagccttc
     1201 ttgggcacgt ctcgtttgac ccataaatac gttctcgaca atgttattac gttcgaattc
     1261 tgagaaactc gcaagtatct gtaacatgag tttaccagaa gaagtattga cttccatacg
     1321 ctctgacaaa ctgaaaaatt cgacattttg tttgtgtaaa tcttcgacaa ttttgagaag
     1381 atcagatgta ttacgagcta atcggtttgt tttgtatacc ataacacagt cgatattgcc
     1441 ttcttttgca tctttcaaca tacgttggag ctcaggtctg ttcatagatt tacctgaaat
     1501 accacggtca gcgtatatat ctttaacttc aaaatgatgg aagtcacagt attctttgat
     1561 ttgattgatt tgtccgtcga tactataacc ttctgtgctt tgcatttctg ttgatacacg
     1621 tacatagata ccgacacgtt ttgttttaag ttgttgcatt atgttacatc ctttcttcat
     1681 ttatgcaatc gatgattgca tggtttgatt gacaatattg agtggttcat ttttgaaata
     1741 gattcctata agatttttat ctttcgtaat gtgaatggtt tcaatatagg ggtacaatat
     1801 gtttaacgtg aaacgttttt gaataatatt ttgaatgatg tgttgtattt gatgcccatt
     1861 gatagatgta gtgcgttgcg gttgttgacg taatgattgt gtttgctctc tgaacgtttc
     1921 tgcatcaatg atgccttgtg ccaacttttc tatcagttgt tcttgagtca atgtgtgatg
     1981 tttttctatg tttctttgtc ttttgatgcg tttgtcaatc gcacttttaa tttttgtgta
     2041 gatgcgttga ttttgataaa agtctcggca cacttctaat actttatctt caagtgtttg
     2101 tgcattgatg cctttaaaat cacagacaaa gcgtgaagca ttcatgtttt taggacagac
     2161 gtagtaacgt aatgtatgat tcttttttct aatggtcata tttgtaagtg ttgcattaca
     2221 acatgggcat ttgatttttt gtttgagttg attatccgaa ggtgtctgtt tggtttgttt
     2281 ttgcaatcga agtctctgcg cttgctcata tatacttgtg gaaacaatag aaggaaacat
     2341 attgtcgaat tggccatatt gattgttgac acgaccacaa taattaggat tgatgataat
     2401 gttacgaact tgatagggtt gtcgattgat atacgtgtta tcttcttcta ataactgtgc
     2461 aattttctta taaccatgac ctttaatgta ataattgaat acagccttta ccgttggtga
     2521 ctcattttga ttgatgatga atgttccgtt gtgatattcg taaccaaaag gtgcatgtgt
     2581 tgtaatcaat cgaccttgct ttgctttttc ttgaagccca tttctgactt gttctccaat
     2641 gttatccgat tcaagttcgg ctaaactgat gaagatatta agcttgagtc ggtcgaaagc
     2701 ttgatccata tcaaagtaac catcatgtac gcttaagata tgaacatggt acgtttgaca
     2761 taatttgatg agttttaatg catttttcag attacgatgc aacctattaa gacgataaca
     2821 gcataatatg tcacactgtc cttgttgaat taattgtgtt atttgtcgat acccactacg
     2881 attatctttg cgacctgatt gtttgtcgct ataaaagttg atatgttgaa tatgatgttt
     2941 ttcggctatt gcttcgatag cttgtttctg tgctgcaaga gattgttgtt tcatcgtact
     3001 ttgacgtaaa tagcctatga cttgtttcat atcggctcct cctttcacag tgataatata
     3061 tatttatgga tgaattgata tataagccca acatcaatga gatgttgggc gtccatatta
     3121 gtcatttgtt tgattgattt cttcaattac caaatcggct aatatctcga taagttcatc
     3181 catgtttttc actccgttat ttgttctatc ttcaatacgt cgattattca gtttgatgct
     3241 tcacagttgt atgataaaga caattagaaa tcttcgtgaa ctcctgaagg gcctatccct
     3301 tcattagcgg atttaaaaag ttctttcgca gctttgttat catttgacgg tgtccaattt
     3361 tgaagtaacg acttatcttt agttaatccg aggatagatg caaactctac atctaatttt
     3421 agatggtaaa atacaagtga ttgtttttta ccgctattat ctttgacact tcttttagtt
     3481 gtttggcgtc cacggtcagc taatatgaaa cctttatctc ttaaggcgtt gacaacatta
     3541 ttaacatctt gaaattgatg attgtttagc atctgtttaa aaacgttcgc aattattttt
     3601 acttcgatat ggtcatcttt taatgagatt aatccatagt tctcaaacat atttttcaaa
     3661 gcaccttcat ctgaaaactt acctctgttt tgtgccacaa attgaatgat gacatcaata
     3721 gctttatcag ctaatgagcg ttcagagact gtatgagtat gataatcaat aaagtagtct
     3781 cttatattag cgatatcaat atctgtagat aaaacacgac ctaatatttt cgcagatgtt
     3841 gtaatgactg cataacgctt aaacatacga atacctgtat tgtttgtttc atctttcaat
     3901 ttagcttcaa accaatctac ttccttgtaa aaccattgaa taacttcatc ttcacgattt
     3961 ataagatatt tagctactaa cggtaaaaca tgaccatagt ttagtgctac agctttttta
     4021 atattgtcag cattggtcgc atttgtagtg aattgttcat taatctcgat ggttcttaca
     4081 cgtaatccat cgttttgagc tgaatcatta aaaatactgt attctgacgt tgaaatgaca
     4141 gaagtacccc aattcttagg cgttttaact tctccatgaa cgtttgaacg ttgacgacct
     4201 tgaccttcag cgatggaata taacaaaccc gtggtatctc taagtgtcgt agatgaaagc
     4261 tcatcaaata ctataggaat gccataattg ttactcaaat aaccttcaag tgcgtttcgt
     4321 gtggcattcc aacttctaaa aagagtttca ttacctttgg tagggttacc agcgactgat
     4381 acagctaaag cagctgcggt tgacttaccg gttgaggatt gacctgtaaa actaaagaga
     4441 attcctgcaa attcgatttc atgtttatgc ttcagaaaac ttgtcactaa ggcagaaatc
     4501 ccaaatatga ctgccaattc taaaagaaga tgacctttaa cctcgtcaat atacatgtta
     4561 aaccaattat caaatgtacc tttaggagtt aagtcataag cactatctac aatagggtat
     4621 gaaggtgagg actgattaaa ttgaatagat ttgagaactt tatctagcga tatcaagtag
     4681 ccaaaaggtg tctctagtat acctacaccc tcatataatt cagaaagtgg taatctatct
     4741 cgcatcaatt gcaaggcata actcaatgat ctaatatact tctcgttaat actgtagcca
     4801 tatttaatta aagaaggtaa gtttcgtgtt gttaagatat cagactcaaa aatgtctttt
     4861 ttaccattgt tgttagtaat aatcaacttt tcaacaccag taattggatc agaaaatctt
     4921 gcattaacaa taatagggct agccattcta accacttttt cactgtcacc atctttttta
     4981 ggtggtatcg tttctttcca accgaaggaa tctaaagcaa atgggccaat ttcaaacaat
     5041 gtgtaactca ttagcaatca cctcctttcg aaggcgtgct attattgcat gggtttatcc
     5101 ctattttctg atataataaa ctgccatttt ttgttcctac aattttaaat ggtaatcctg
     5161 gcgtgtactt aatccagtaa gcaaaacgac gtcctacttc acgttgatca ttcgtgctac
     5221 aaaccacatt atatttcttt cttaattcac cataagtgaa cgtacttcca actggttgtt
     5281 gaaaagattt agcaattata aaattataaa gcttttcttg ctgcttagat ttcttattca
     5341 ttttaaaatt cctccaataa tttttaagga gtcaatcaat atcatatagg ttaaactatc
     5401 ttcaattgat tgctataata agcataaatg aagcattatg attagacgcg gaacaaaaaa
     5461 tggggagggg gggtatatat tgatacctac gctagaagaa attattgata agtatggaaa
     5521 cttagttgat tatttgaaat ttgatgtaac agttgaggtt tatgaagatt tgttgttatt
     5581 aagaagttta agcgatttaa atgaacatca aaagatggat agagtttcat ttataaaaaa
     5641 acatttaaac tcaaatttcg attatttatt agatgatact gagtttagaa gtatcaataa
     5701 gatttacagt aatttaaata tcatgacgca tatcaatagt caaaataata attttaaacg
     5761 taatccattt ataaatgaag aacaattgaa atcactactc gcgataaaga aagtagatgc
     5821 acatatgagt tatgattcaa atattacttc aaaagcattg gcagaaataa acaaaacgca
     5881 aaaaagatta gttacaaaga tagacacact ctataatcag tcaaaaaaag aaaatgaata
     5941 tgtacggtta ggagaaaaaa tatcgtatac aaaattggag aatcattgga aatatttata
     6001 taatgagatt cagttttata atcttaataa tcaattgatt agttatgttg cattagaaca
     6061 ggaatatgct tgggcttttt taaatgaact attttatttg atagaattgt atttgaaggc
     6121 atttaaagga caaaa
//
LOCUS       DAKTLH010000147         6045 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.149, whole genome shotgun sequence.
ACCESSION   DAKTLH010000147 DAKTLH010000000
VERSION     DAKTLH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.149"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            391..1557
                     /gene="aroC"
                     /locus_tag="O0394_002321"
     CDS             391..1557
                     /gene="aroC"
                     /locus_tag="O0394_002321"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCY0500386.1"
                     /translation="MRYLTSGESHGPQLTVIVEGVPANIEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDSIAQAMRDKIDEAKNEGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGKIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNSEIGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLKQQIIERRQLNIEF"
     gene            1583..2647
                     /locus_tag="O0394_002322"
     CDS             1583..2647
                     /locus_tag="O0394_002322"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCY0500387.1"
                     /translation="MKLQTTYPSNNYPIYVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFDDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFIAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESATQDIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVIADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFD
                     SKHDINHYIQYLIQLGYPLDMITDLDFETLYQYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            2657..3955
                     /gene="aroA"
                     /locus_tag="O0394_002323"
     CDS             2657..3955
                     /gene="aroA"
                     /locus_tag="O0394_002323"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCY0500388.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
                     YQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTT
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            3962..5206
                     /locus_tag="O0394_002324"
     CDS             3962..5206
                     /locus_tag="O0394_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500389.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEALTTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            5220..5795
                     /locus_tag="O0394_002325"
     CDS             5220..5795
                     /locus_tag="O0394_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCY0500390.1"
                     /translation="MSETFNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDN"
     gene            5785..>6045
                     /locus_tag="O0394_002326"
     CDS             5785..>6045
                     /locus_tag="O0394_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HCY0500391.1"
                     /translation="MTIKAFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPDSPTAILYLVISISLILIQKQNSIIDALAFVTLFKY"
BASE COUNT         2160 a          837 c         1123 g         1925 t
ORIGIN      
        1 cttaacatat gtatatttta atatcataat ttttttaaac ggactgatta actttattaa
       61 taattaacag ttcgttcttt tgtattaaga aatgtagtca gtatattatt tgctaaagtt
      121 gcgatacgat tatattaaaa cggctaatca tttttaatta atgattatat gatgcaactg
      181 tttagaaatt catgatactt ttctacagac gaatatatta taattaattt tagttcgttt
      241 aatattaaga taattctgac atttaaaatg agatgtcatc cattttctta attgagcttg
      301 aaaacaaaca tttatgaatg cacaatgaat atgataagat taacaacata ttataatgtt
      361 atcgtggaag tatgaaagga gcgagtgtgt atgagatacc taacatcagg agaatcacat
      421 ggacctcaat taacagttat tgttgaaggt gtacctgcaa atatagaaat taaggttgag
      481 gatattaata aagaaatgtt taagcgtcaa ggcggttacg gacgtggacg tcgtatgcaa
      541 attgagaaag atacagtaga aatagtatca ggcgttagaa atggttatac attaggtagt
      601 ccaattacta tggttgtaac caatgatgac tttacgcatt ggagaaaaat tatgggagca
      661 gctccaataa gtgaagaaga acgtgaaaat atgaaacgta ctattacaaa accaagacct
      721 ggtcatgcag atttggttgg aggtatgaaa tataatcatc gtgatttacg aaatgtgcta
      781 gagcgatcat ctgctagaga aacagcagct cgagttgcag tcggtgcctt atgtaaagtg
      841 ttattacaac agttagatat cgatatatac agtcgtgttg ttgaaatagg tggaattaaa
      901 gataaagatt tttatgattc agaaacattt aaagcaaatc ttgatcgtaa tgatgttcgt
      961 gtaattgatg acagtatcgc acaagcaatg cgagataaaa ttgacgaagc taaaaatgaa
     1021 ggagattcaa ttggcggtgt cgttcaagtt gtagttgaaa atatgcctgt tggtgtaggt
     1081 agttatgtgc attatgatcg taagttagat ggtaagattg cacaaggtgt tgtcagcata
     1141 aatgctttta aaggtgtaag ctttggtgaa ggatttaaag cagctgaaaa gccaggtagt
     1201 gagattcaag atgaaattct atataatagt gaaattggtt attatcgtgg atctaatcac
     1261 ttaggtggtt tagaaggcgg tatgtcaaat ggaatgccaa ttatcgttaa tggtgtaatg
     1321 aaaccaattc caacgttata taaaccatta aattcagtag acattaatac taaagaagac
     1381 tttaaagcaa caattgaacg ttctgatagt tgtgctgttc ctgcagcaag tatcgtctgc
     1441 gaacatgtcg tagcatttga aatagcaaaa gcattattgg aagaattcca atcaaatcat
     1501 attgagcaac ttaaacaaca aattattgag cgcagacaat taaatattga gttttaacaa
     1561 caagaacaat tgaggtgtaa tcatgaaatt acaaacaaca tacccttcaa ataattatcc
     1621 aatatatgtt gaacacggtg caattgacca tattagcacg tatattgatc agtttgatca
     1681 aagttttata ttaattgacg agcatgtaaa tcaatatttt gctgataaat ttgatgatat
     1741 tttatcatat gaaaatgtac ataaagttat tattccagct ggtgaaaaga cgaaaacatt
     1801 tgagcaatat caagaaacat tagagtatat tttatcccat catgtaactc gtaatacagc
     1861 aattattgct gttggtggtg gtgcaactgg tgattttgct gggtttattg cggcgacact
     1921 tttacgaggc gttcacttta tacaagtgcc aacgactata ctagcgcatg attctagtgt
     1981 tggcggtaaa gtgggtatta actcaaagca aggtaaaaac cttatcggtg cattttatcg
     2041 tccaactgct gtgatttatg atttagactt tttaaagacg ttaccatttg agcaaatatt
     2101 aagtggctat gcagaagttt ataagcatgc gttattgaat ggtgaatcag cgacgcaaga
     2161 tatcgaacag cactttaaag atagagagat attacagtca ttaaatggta tggataaata
     2221 tattgctaaa ggtattgaaa cgaagctgga tattgttatt gcagatgaaa aagaacaagg
     2281 tgtacgtaaa tttttaaatt taggtcatac atttggtcat gctgttgaat actatcataa
     2341 aatacctcat ggtcatgcag tgatggttgg cattatctat caatttatag ttgcgaatgc
     2401 tttgtttgat tctaagcatg atattaatca ttatattcaa tatttaatac aactcggcta
     2461 tcctttagac atgataactg acttggattt tgaaacgtta taccaatata tgctaagtga
     2521 taaaaagaat gataagcaag gtgtacaaat ggtcttgatt agacaatttg gagatatcgt
     2581 tgtacaacat gttgatcaac taacattaca acatgcatgt gaacaattaa aaacatattt
     2641 taagtaggtg aatgaaatgg taaatgaaca aatcattgat atttcaggtc cgttaaaggg
     2701 cgaaatagaa gtgccgggcg ataagtcaat gacacaccgt gcaatcatgt tggcgtcgct
     2761 agctgaaggt gtatctacta tatataagcc actacttggc gaagattgtc gtcgtacgat
     2821 ggacattttc cgactgttag gtgtagaaat caaagaagat gatgaaaaat tagttgtgac
     2881 ttccccagga tatcaatctt ttaacacgcc acatcaagta ttgtatacag gtaattctgg
     2941 tacgacaaca cgattattgg caggtttgtt aagtggttta ggtattgaaa gtgttttgtc
     3001 tggcgatgtt tcaattggta aaaggccaat ggatcgtgtc ttgagaccat tgaaacttat
     3061 ggatgcgaat attgaaggta ttgaagataa ttatacacca ttaattatta agccatctgt
     3121 cataaaaggt ataaattatc aaatggaagt tgcaagtgca caagtaaaaa gtgccatttt
     3181 atttgcaagt ttgttttcta aggaaccgac catcattaaa gaattagatg taagtcgaaa
     3241 tcatactgag acgatgttca aacattttaa tattccaatt gaagcagaag ggttatcaat
     3301 taatacaacc cctgaagcaa ttcgatacat taaacctgca gattttcatg ttcctggcga
     3361 tatttcatct gcagcgttct ttattgttgc agcacttatc acaccaggaa gtgatgtaac
     3421 aattcataat gttggaatca atccaacacg ttcaggtatt attgatattg ttgaaaaaat
     3481 gggcggtaat atccaacttt tcaatcaaac aactggtgct gaacctactg cttctattcg
     3541 tattcaatac acaccaatgc ttcaaccaat aacaatcgaa ggagaattag ttccaaaagc
     3601 aattgatgaa ctgcctgtaa tagcattact ttgtacacaa gcagttggca cgagtacaat
     3661 taaagatgcc gaggaattaa aagtaaaaga aacaaataga attgatacaa cggctgatat
     3721 gttaaacttg ttagggtttg aattacaacc aactaatgat ggattgatta ttcatccgtc
     3781 agaatttaaa acaaatgcaa cagttgatag tttaactgat catcgaatag gaatgatgct
     3841 tgcagttgct tctctacttt caagcgagcc tgtcaaaatc aaacaatttg atgctgtaaa
     3901 tgtatcattt ccaggatttt taccaaaact aaagctttta gaaaatgagg gataatataa
     3961 aatggaagat atctataaat taatagacga tatcaatcta caaaaactag aaaatttaga
     4021 ctctcgtgtt aatgaagcac taactactga caacgatgac gcattattta ttctaggaga
     4081 gacactttac aattttggat taatgccaca aggtttggaa gtattccgcg tgttatatca
     4141 caaatatcca gacgaaagtg aattgctgat ttattttatt gaaggtttaa tgtctgaaaa
     4201 tcaaactgac gaagcgttag aatatttaag ttatgttgaa ccatcacctg aaaagttgat
     4261 gttagaagca gatttatatc aacaaattaa tatgatggaa gttgctattg ataaattaca
     4321 agaagcactt gaactagagc caaatgatcc aataatccat tttgcattgg ctgaaatgtt
     4381 atattatgat ggtcaatatt tacgtgctac ctctgaatac gaaaccgttt tagaaactgg
     4441 tgaatatcaa gttaatggtg taaacttatt ctctcgtatg gcagattgta gtttacaaag
     4501 tggtaactat agtgatgcga ttcgcttata cgatgaaatt aatgaagatg aaatgacttc
     4561 agaagattat ctcaaaaaag ccatttctta cgataaaaat gacatcactc aagaagcaat
     4621 taaaataatg actacattac tttctaaaga tcctgattat attcaaggct acttgtattt
     4681 acaatcatta tatgaaaatg aaaaaaatta tccagatgcc attgaaacgg gtaaagaagg
     4741 attacgacta agtcaatttt ataaagagtt aatgtataca actggatgtt tggaaataga
     4801 acacggcgat gctaatgaag gtgtattatt gttaaaacaa gcattagagg ttgataatgc
     4861 ttaccaagaa cctttattga ttttaagcga tttatatcgt aatgaagaag attatgaagc
     4921 aatcattgaa ttattaacat atgtagatga agaagatttg gatcctacat tcatgtggca
     4981 tttagcgttc gcctatggtc aagaagagcg agataaagaa gctcaacatt tctttgaatt
     5041 agcatatcca acgatgaaaa caaatattga ttttatgagt gattattatt tctatttaat
     5101 tgaaattggt caaaaggaac aagcaattac tattttaaat caattgttgg aactagagcc
     5161 aagtaacgaa aattggcatg acgaattatc aagattgcaa tcttaaaatg gggtatttga
     5221 tgtcagagac attcaatcaa attaaagaaa gttttattga atatttactt tttcaatatc
     5281 gatttaagtc gcgtattgca gtttgggtgc ttaactatat aaaagtgaat gaggcgaaat
     5341 tggcaaacat tcattttgta gatacaaaaa ttaatcatca tgaaacatta gaaattgcag
     5401 aagtgggaag tcacgcttct gcaattcaat ttactaagcg taatataaag ttaatgaata
     5461 caaatgaaat atttgattat atcgctaatc ataactgtgc ttttgatatt caaattcact
     5521 tcgcaaacgt gtcaaagcgt gagcaaaggc tagatgattt aatagttgct caactaacgg
     5581 aatcgcccag ttatcaaact tatcttcatg acttaaatag tatggctata gatcggcata
     5641 aacatgcgtt attaatagat tacttgttac acaatattga tttaagttta cagatgaatg
     5701 aaaagcaacg tttttatcaa ctaacgcaaa ttttaaatac actcaagcta gtaaataaac
     5761 ataatcaatt tgaggactta gcagatgaca attaaagcat tttggcaata tacactttat
     5821 caacgttctt ggttaatgat gctactcatt tgtaatatat tagggatgat ttatggttat
     5881 atatggtatg gtgaacaatt aagtcatacg ccctggcatt ttaagatttt tgttccagat
     5941 agcccaactg caatattata cttagttatt tctatctcat tgatattaat tcaaaaacaa
     6001 aatagtatta tcgatgcact ggcatttgta acattattca aatat
//
LOCUS       DAKTLH010000148         6009 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.150, whole genome shotgun sequence.
ACCESSION   DAKTLH010000148 DAKTLH010000000
VERSION     DAKTLH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.150"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            277..591
                     /locus_tag="O0394_002327"
     CDS             277..591
                     /locus_tag="O0394_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCY0500392.1"
                     /translation="MTYKELATFLKVLSDSSRLEILDLLSCGELCACDLLAHFQFSQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTG
                     EC"
     gene            591..1883
                     /gene="arsB"
                     /locus_tag="O0394_002328"
     CDS             591..1883
                     /gene="arsB"
                     /locus_tag="O0394_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HCY0500393.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHVGFVRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF"
     gene            1901..2296
                     /gene="arsC"
                     /locus_tag="O0394_002329"
     CDS             1901..2296
                     /gene="arsC"
                     /locus_tag="O0394_002329"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HCY0500394.1"
                     /translation="MTKKTIYFICTGNSCRSQMAEGWAKQILADDWNVYSAGIETHGV
                     NPKAIEAMKEVGIDISNHTSDLIDNNIIKNSNLVVTLCSDADVNCPSLPTNVKKEHWG
                     FDDPAGKPWSEFQRVRDEIKIAIENFKSR"
     gene            complement(2591..3445)
                     /locus_tag="O0394_002330"
     CDS             complement(2591..3445)
                     /locus_tag="O0394_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCY0500395.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(3721..3945)
                     /locus_tag="O0394_002331"
     CDS             complement(3721..3945)
                     /locus_tag="O0394_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500396.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
     gene            4144..4614
                     /gene="sigS"
                     /locus_tag="O0394_002332"
     CDS             4144..4614
                     /gene="sigS"
                     /locus_tag="O0394_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
                     /protein_id="HCY0500397.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSKQSLSSFLFTRLNFYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRL
                     QDIFKLLNQRERLWLKLYLEGYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN"
     gene            4727..5170
                     /locus_tag="O0394_002333"
     CDS             4727..5170
                     /locus_tag="O0394_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCY0500398.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILKVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINPKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHFIENTISN"
     gene            complement(5157..5600)
                     /locus_tag="O0394_002334"
     CDS             complement(5157..5600)
                     /locus_tag="O0394_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500399.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPV
                     TFANAYLRQNVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPK
                     VDLSTTVVILTIIGAILLMFASIYFIKIVTSYFTKKLLEIKFNSK"
     gene            5896..>6009
                     /locus_tag="O0394_002335"
     CDS             5896..>6009
                     /locus_tag="O0394_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCY0500400.1"
                     /translation="MQKFKDFFYDDLSVTRGNYFLTLMAAFFITIILFIGIV"
BASE COUNT         2049 a          954 c          816 g         2190 t
ORIGIN      
        1 tttagtcgta aaaacaaagc ttcttaatca atacccttga ttacaaaata aattcaacta
       61 aggtgattgg ccatacactc aagatatggc taatcacctt tttcaatgca gaacaaagag
      121 tatattttgc aaaatcaata tctataattt atctttaata cccttttaat actaaaacca
      181 aatttaaatt acatagtcat atcgtgtaat ataccaaagg aacatagaca ttcatctata
      241 tagatgataa tctatttaac gtctaggagg tatgttatga cgtataaaga actagcaaca
      301 tttttaaaag ttttatcaga ttcaagcaga ttagaaatac tagatttact ttcttgtgga
      361 gagttatgcg cttgtgattt gttagcacat tttcaattct ctcaacctac acttagctat
      421 catatgaaag cattagtaaa aaccaactta gttacgacac gaaaaatcgg aaataaacat
      481 ttataccagc tcaatcataa tatttttgag tccgtaatta ataacttgtc taaggttcat
      541 acctctaacc aacgatgtat ttgtcataac cttaagactg gtgaatgcta atgatgacaa
      601 ctttagcgac actcattttt ctagtaactt tattatttgt attatggcaa cctaaaggcc
      661 tagatattgg cattaccgca ctaactggtg cctttattgc tgttattact ggtgttgtaa
      721 gtttttccga tgttttcgaa gtaacaggta ttgtttggaa tgctactttg acttttgtct
      781 cagtcattct tatttcatta atattagata aagttggatt attcgaatgg tcagctattc
      841 acatgcttca tgcttcaaaa ggcaatggtt taaaaatgtt cgtttatatc atattattgg
      901 gtgccattgt tgctgcattt ttcgcaaatg atggcgcagc gttaatctta acgcctattg
      961 tattagcgat ggttaaaaat ataggtttta gtaagcgggc catattccct tttattattg
     1021 cgagtggttt tatagctgac acaacttctt tacctttaat cgtgagcaat ctagtgaata
     1081 ttatatctgc tgattatttt catgtaggat tcgttcgata ttttagtaga atgattatac
     1141 ctaatttatt ctcgctttta gcaagtatta tagtattgtg gttatatttt agaaaagcga
     1201 tacctaaaac gtttgatgat aataatataa agcatcctaa agatgccatt aatgatttaa
     1261 agctatttaa aatttcatgg attgttctag ttatattact tttcggctat ctaatcagtg
     1321 aatttactaa aattccggta tcaattttca ctggaatcat tgcttttatt tttctaatgt
     1381 tggctcgtaa atcaaatgct gtaaatatta agcaagtcat taagggcgca ccttggaata
     1441 tagtattatt ttcaattggt atgtatatcg tcgtattcgg cttaagaaat gctggcatta
     1501 ctttaatatt ggctaaaata ttagaatata tttccaatta cggtctattt agcactattt
     1561 tgggaatggg cttcatttca gcgtttttat catcaataat gaataatatg cctacagttt
     1621 taatagatgc gattgctatt ggtcaatcaa atgtccatgg catgttaaaa gaaggcctaa
     1681 tttatgcgaa tgttatcggt tctgatttag gtccaaaaat tacaccgata ggctctttag
     1741 ctacattact gtggttacac gtcttaacac aaaaagatgt taagatttct tggggcacat
     1801 actttaaaac tggtatcatc attacaattc cagtactatt tataaccctc atagggttgt
     1861 atctaacact tatcatattt taattaatta ggagattgtt atgactaaaa aaacaattta
     1921 ttttatatgt acaggcaact catgtcgaag tcaaatggct gaaggttggg ctaaacaaat
     1981 cttagcggat gattggaatg tatattctgc tggtatcgaa acacacggtg ttaatcccaa
     2041 agcgatagaa gctatgaaag aagtaggcat tgatatatca aatcatacat cagatttaat
     2101 cgataataat attattaaaa attcaaattt agttgttaca ttatgtagtg atgcagacgt
     2161 aaattgccct tctttaccaa caaatgttaa gaaagaacat tggggatttg atgatcctgc
     2221 aggcaagcct tggtcagagt tccaacgtgt aagagatgaa attaaaatcg caattgaaaa
     2281 tttcaaatca cgatgagagt gggacagaaa tgataaaaaa acactaatga tttattatgt
     2341 agtggttctt tatcattagc tataactaat gtgtacttaa aaataggaat acatgagtaa
     2401 aactcatgca taagaaatac taatttctaa agaaaaagta attctttatc gatgtcctgc
     2461 cccacttgca ttggtgggat tatcaatcaa tgtgttttct ctaatgcaaa aagacgcttt
     2521 ctatcaaaac aagatagaaa gcgtcttttt agtatgtccg agtcataaaa acaactcgta
     2581 gcttatcaaa ttacttattc aaatgtttac tgtcatcttt atacacaaag tatttgaagt
     2641 attttccttc agtcttcatg ttcttataaa agtcagcgat aattgttgca ttactttctg
     2701 cccacttaat atctgtagca tattgatgtt ctcctggatt ttttggattc catctcatac
     2761 tatacaatgt attttgatct gtgcttgata agaagtgctt atgaatgaaa tcagcaccgc
     2821 ctgaaatagc tttttcaggt gtatcccaac catgcttttt agcatattct gcacctgttt
     2881 taattgggtc tttatcaagg gctcctactc catagaaatt gtagtacttt ttgccatcaa
     2941 tttcgactcc attagctaat tcacttttaa ctgcgccagt ttctaataat gcatgtgaaa
     3001 ttaaataaac ttcgttaacg tgcttatctt tagcagcttt taagaaatca tccgtatgtt
     3061 tcaataacgt tggtctatct actaacatac gtttaattct atttttatca atcccttgat
     3121 actttgataa atctaaaaat tgatattttt gcttttcatt atcgataaaa gtaccgctat
     3181 ccattgcact tttaatttca gttgcagatg catctctcca tgcatcattc tttttatttg
     3241 atacctgttg actcgtataa ttatttattt gtttctttgc tgcatcgttt aatgtaacat
     3301 ttaacttttc aatcttaatg tcggatttaa catgtttgaa aaatatctga tcggatatca
     3361 ttgagaaaaa taaaaatgag acaacagcaa atatgacaac aagtcctatt attccaaaaa
     3421 tagaaccttt cttgtgttta ttcatatcca cacctcttag gtcattgttg ttatacattc
     3481 agtttgacta cgataataaa gtttaacatt attaaaaccg tttaacaatt ttatctatgc
     3541 attgttacat attcataaat aaacagtaat gtaaatgaaa cgttttaaac catttatcaa
     3601 cattacttta cgttgatcta cgaatcataa atgttttatc tttttgtata acctttgaaa
     3661 acctttatat caataatctt attattcacg tattaaaaaa caaaaaattc ttttgttcgt
     3721 ttacttagtt tcttttttat aatccatcat aataaaactt gctgcatttg atctattttt
     3781 ataatagtac tgatttaaat caatcgtgcc ttcaatttga ccatcacgat acatcatttc
     3841 attagtgaag ttatttatca tcacgaaatc agcgcgatct cttataacat ctaaatcgtc
     3901 gtttcgtgca aaaaaatgtt ctaaatttaa atgtgcgtaa tccattgtta cctcccgatt
     3961 acaaattttg ttctatctac tatttagcaa agtaataata catcatatca cattaaatta
     4021 attacttatg tcttatattt aacacttaat ttagcgatta cgcaaatgaa tgatttttct
     4081 aaatatttta ttctaacttt atccggaaat atttgttata cgtataactt ggagaatgac
     4141 tacttgaaat ttaatgacgt atacaacaaa caccacaaaa tcatacacca tcttttaaaa
     4201 aaatataata ttagctataa ttatgatgag tattatcaac tactcttgat aaaaatgtgg
     4261 caattgagtc agatatataa accctcaagc aagcaatctt tatcctcttt tttattcact
     4321 cgattaaatt tttaccttat cgatttattc agacaacaaa atcaattaaa agatgtcatt
     4381 ttatgtgaga ataattcacc aacattaact gaacaaccaa catactttaa tgaacatgac
     4441 cttcgtttac aagatatctt caagctttta aatcaaagag aaagactatg gctcaaacta
     4501 taccttgaag gatacaagca atttgaaatt gctgaaatca tgtcattatc gctttcaacg
     4561 attaaattaa ttaagatgtc cgttaagcgt aaatgccaac ataattttaa ttagtttatt
     4621 gtgaaatttc ggtcgatgtg ttaatgatgt tgaaacaccc taacatcttc atataaattt
     4681 attttaacca ttaaattaac aacttattaa acgaggagta ctatatttgc aagacaattc
     4741 tactaaatat ctactttata tccaaactgc tacttcaaac catctcgaaa caaattgtgt
     4801 ctttttacat tgcgattaca ttcttaaagt tccaattaac aaactcgttt catattatgc
     4861 gaaattgcat ttatcatcac aaagtgtgct aattgagact gcaaaaaaca tactaaatat
     4921 taataaactg gttcctattt acatcaaccc aaaaactata ctttttccgt taaaacataa
     4981 acgtgcaccg atacaaattt atatcaatgc acattatatt gttggtatga ctgccataga
     5041 gaattcaaca ttaatacatt ttcaagaagg cattcagcta gaagttgatg agccattttc
     5101 tctagtttca aaaaaatgtc atgaaagttt agctttgaag cacttcattg aaaatactat
     5161 ttcgaattaa atttaatttc taaaagtttc ttagtaaagt atgatgtcac aatttttata
     5221 aaatatatgc tagcaaacat taataaaatc gctccaataa ttgttaaaat aacaacagtt
     5281 gtagataaat ctaccttagg actaacgaaa aattgtacgc cagatagact tgtaattagc
     5341 ccaaataaaa acaaacctaa taaagcaaca taagcaaaca tcatcactac taatgtaaca
     5401 aacatactat aactttttgt ttctttattt cgaaatacat tttgtcttaa gtaagcatta
     5461 gcaaaggtaa caggcttatt taagttaacg ctgtcatcat cagatataga agacaatcgt
     5521 tgatctaatg cttgtttctc cttcttgttc aaaaaccata attgcttttt aactttttta
     5581 tcaaatgatg ctttattcat aattaaggat ttctctcctt tataatattg acgtaatttt
     5641 aaacattagc gtcaattgaa agtgtatttt atatatttta cagaaatata tgtatcaaat
     5701 ctacaaaaat ccatattaca atttcattta tggtgatttg acgctaaaat acagtctgct
     5761 aatttttcaa actaatgctt tttaggtagg tacaatgata aattttaatc gtaaatatgt
     5821 ttcatatatt atgaatctag ccttaatcat attgcattca aaataatttt aaaaaatgaa
     5881 aagaaaggat ttagcatgca aaaattcaaa gacttttttt acgatgattt atcggttaca
     5941 cgaggaaatt attttttaac tttaatggca gcatttttta ttactatcat tttatttatc
     6001 ggcatagtt
//
LOCUS       DAKTLH010000149         6007 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.151, whole genome shotgun sequence.
ACCESSION   DAKTLH010000149 DAKTLH010000000
VERSION     DAKTLH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.151"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            222..1160
                     /locus_tag="O0394_002336"
     CDS             222..1160
                     /locus_tag="O0394_002336"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCY0500401.1"
                     /translation="MANIVFKLSDKDITTLMSRISFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSEELLPQHSQLNTNKTKKKNMANSFLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAALHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKKYGLSRLDTISKKHFKNREKAQKILKPL"
     gene            complement(1331..3733)
                     /locus_tag="O0394_002337"
     CDS             complement(1331..3733)
                     /locus_tag="O0394_002337"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCY0500402.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSEAQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELDLSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     QTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVLVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFDKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFSMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQDKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPILAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(3733..4791)
                     /gene="pheS"
                     /locus_tag="O0394_002338"
     CDS             complement(3733..4791)
                     /gene="pheS"
                     /locus_tag="O0394_002338"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCY0500403.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEDKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(5172..5912)
                     /locus_tag="O0394_002339"
     CDS             complement(5172..5912)
                     /locus_tag="O0394_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCY0500404.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPHYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
BASE COUNT         1928 a         1161 c          857 g         2061 t
ORIGIN      
        1 ttataatgaa ataatgtaaa gaattttcta ttcaatactt tatcatgttt aaattgtgtc
       61 actataacat tttcataaac attatacatg accactatgt attttgtaag tatccgcaat
      121 taattcttta caacatacat aaatgtttct gacgttatta tcatttatga tatgattatt
      181 tttgctaaag acaatgaaat tttatgaaag gatttacaca aatggcgaat atcgttttta
      241 aattgtcgga taaagacata acgacattaa tgtcacgcat ttcttttgat actgagaatt
      301 tacctcaagg tatgaaagca cgtgcaaagt atcaaaatac aactgtaaat atttaccaat
      361 caggtaaagt catgtttcaa ggtaatcatg cagaagctgt gtctgaagaa cttctaccgc
      421 agcattctca attgaatact aataaaacta aaaagaaaaa tatggctaat tcttttcttg
      481 aacaaacttt aatgtatgat caatttaact gtattggtag tgatgaggct ggaagtggcg
      541 attactttgg tccgttgact gtgtgtgctg ctttcgttac caaagagcat gtccctattt
      601 taaaaacttt gggtgtagat gattcaaaaa aattaaccga taccaaaatt gtagaacttg
      661 ctgagcaatt ggttactttc ataccacatt cattattaac actacacaat gaaaaatata
      721 atattcagca agcaaaaggc tggactcaag taaaaatgaa agctgccctt cataatgaag
      781 ccatcaaaaa tgtacttgaa aaaattgatt cgtctcaatt agactatatc gtcattgatc
      841 agttcgcaaa acgcgaagtg tatagccatt acgcattatc agatatacca cttccaaaga
      901 aaacaaaatt tgaaacaaaa ggagaatcga agtcattagc cattgcagtg gcaagcataa
      961 tttctcgata cgcatttgtt acatatatgg atcaaatatc aaaaaatatt aatatgacga
     1021 ttcctaaagg tgccggtgct aaagtcgatg taatcgccgc caaaattata aaaaaatacg
     1081 gtctttcacg tttagatacc atttcaaaaa agcattttaa aaatcgtgaa aaagcccaaa
     1141 aaattttaaa gcctctttga tttttaatca ttagaggctt ttatgcagtt gtgcgaagca
     1201 tcatctgtat tccgctgttc ctctttgact ttttaaatgc attggaacca aattggtgaa
     1261 cccaaaaata aaaccccatg ttagggatat caatctactt cagatatcct tatacatggg
     1321 gtttaaatca ttatctaata acagcacctt gttcaattaa tgctgcttca atttccgctt
     1381 gtacttttga aacgcgctca tctgtcaatg tttcttctgt gtctaaataa tttaaacgta
     1441 ttgcaattga ttttttacct ttttctaaat gttcgccctg atatacatca aagacaagtg
     1501 tatcttttaa tatattgcca ccatgtgcat gaatcgttga taataaatca gctgctggaa
     1561 tattttgatc tacttctaat gcaatgtcac gagacatgcc tgggaatctc ggaattggct
     1621 ggtaattaat gtaacctacc gacacagcca ttaatgcatc aaaattcaac tcaaaaacat
     1681 acgtacgttt taaatcatta tcagctgcta atattggatg taattcacca ataaaaccaa
     1741 caactttatt ctctaataag atttcagcag tacgacctgg atgtaatcca tcaatatcag
     1801 cacggcgata actaaattca agatttaact tttcagatac tcgatccacg acaccttttg
     1861 ctaaatagaa atcaaccgtt tctttcttat cttgccattg attgactaca taatctccag
     1921 ttaaaatacc acttaaatat tcaacttgat ctggtagttc accttctcca ttagcaaaga
     1981 agacattgcc gatttcaaat aattttacat ctttattttt gcgtgccaca ttatatgatg
     2041 ccgcttcgat taaatgtggt aataaacttt gacgtaatga cgcatgcgct tcactcattg
     2101 gcatcaataa atcaattgtt tgacgctgtt gcatcgaaaa tgcagtagca tcttctttag
     2161 aaactaacga atacgtaata gcttggtcta atccagcacc ttctaacact tctttaacca
     2221 ttctagtttt atattggcga tcagttagct gaccactagt aactttatcg aagacaggta
     2281 acgttgatgg aatatcgtcg tatccatata tacgtgcaac ttcttcaatt aaatcttctt
     2341 taattgtaat atctttacga cgtgatggta ctagcactgt aataacatca tcatttattt
     2401 ctgtatcaaa ccctagttga ttaaaaatag taacaatatc attttgtgac aaatcaaatc
     2461 caatagtgcg attaatttta tcagcagtga tgtcgattgg tgtaataaat gcaccaagtt
     2521 ctcctgaaga cactctatct tttagcactt ttccgtttgc ataagtttgt aataaataac
     2581 atgcacggtc gactgcttca tctacaaatt cagtagctat tcctttttca aaacgactag
     2641 atgattcact gcgtaaattt aaacgtcttg atgtatgacg aattgaaact ggatcaaaaa
     2701 tagcaccttc aatcactata tttgatgttt gttctttaac ttctgaaaaa tcgccaccca
     2761 taacaccagc taatgcaatt ggagtttgtc cattagtaat gacaatatcg ctcgttaata
     2821 attcacgttc tgtatcatct aatgttgtca ttttttcgcc ttcattagct tgacgaacaa
     2881 caatttgttg tgaaccaatc gcatcttgat caaacatgtg caatggttga ccgtattcta
     2941 ataacacata atttgaaatg tcaacaacat tattaatagg acgtataccc gcttttatta
     3001 agcgtgcttg catccaaatt ggcgagggtt caattgtcac gtcgtgaaca acacgtgcac
     3061 tataatatgg tactttatct tcattttcaa ttgtcacagt cagttcatca tttgcagata
     3121 aatcaagctc atttgatgtt gtctctggct tagtcatttt tgtattatat aatgctgcaa
     3181 cttcataagc agtacctatc atacttaaag catctgcacg attcggcgtt aaatcaaatt
     3241 ccattacttg atcatctaaa tataaagctt gtaaggcatc tgttcctgga acttgggctt
     3301 cactaaatac ataaatgcct gattcaaaac ttttcggtat atagttactt gaaataccaa
     3361 tttcttgtaa cgaacaaatc ataccttctg aacgttcacc gcgtaatttg gcacgcttaa
     3421 ttttaatacc accaggcaat ctgccaccta ctttagcaac aatgacatat tgtcctgcat
     3481 caacgttcgg tgcaccacaa acgatttgta caggttcgtc ttctccgata tcaacttggc
     3541 aaacatttaa tttatcagca tcaggatgtt tctcttttga cttaacgaag ccgacaacta
     3601 aatttttgat atcttttgtg tagtcaatta aatcatccac ttcaatacct gtgcgcgtaa
     3661 tacgttctgc caaattactt acagaatcat cgattgttac atattctttc aaccattcat
     3721 ttgatatcaa cattacatgt cacctctatc ttctaccgct ttaaattgat ctaaaaatct
     3781 cacatcatta gtatagaaat gacgaatatc ttctatacca tatttcaaca ttgcaatacg
     3841 gtctggtccc ataccaaatg caaatccaga gtactctgaa gaatcaaaac cagccatttc
     3901 taatacatta ggatgtacca ttccagcacc taaaatttca atccatcctg tgtgtttaca
     3961 cacattacaa ccttttcctt tacatttaaa acatgacaca tcaacttcta cagaaggttc
     4021 agtgaatggg aagtaacttg gacgtaaacg aatttcacga tcagcaccaa ataatttctt
     4081 agctaacaat tctaaagtac ctttcaaatc actcatttta acgtttttat caacaactaa
     4141 tccttcgatt tgtgtaaatt gatgactatg tgtcgcatca tcagagtcac gacgatacac
     4201 tttaccaggg caaataattt taactggacc ttgaccatga cgtgattcca tcgtacgtgc
     4261 ctgcactggt gatgtatgcg tacgtaataa aatttcatcc gtaatataga aactatcttg
     4321 catatcacgt gcagggtgtg atttaggtaa attcagcatt tcgaagttat aatgatcttg
     4381 ttcaacttca tatccattca caatttcata acctaaacct aagaataagt cttcaatttc
     4441 ttctattgta cgtgttaatg gatgctttga accgatttca atatgacgac ctggtaatga
     4501 tacatcaatt gtttcttcag ccaattgctt atttaatttt tctttaacta acatctgttg
     4561 tctttcatct aattcatttt gaattgtttg acgcaattca ttcacttttt gaccaaacgc
     4621 aggtttatct tcattcggca aatccttcat caatttcatt agtccgctaa ctgacccttt
     4681 tttacctaag tatttcactt taacttcttg cagtgcacgt tcatcatttg cttcattaat
     4741 atctacaagc gcttgttgtt ttaactctga cattgtttgt tgttcagaca tatgtcactg
     4801 acctccctat tcgaactatt cattttctat ttatcatatt tgttgtttaa ctaaaaaaag
     4861 actttcatcc tcttaaaaaa taagggacga aagtcatttc cgtggtacca cccaaattta
     4921 tgcctaagca tacacttgtt taatattgat aacggattgt ctttccgact taaatctctt
     4981 taagtaacca aaaaggtgaa aagactttaa ataattggaa taacttgcag tcacgattat
     5041 tctccctaaa acaattattt atataaatgt ccatgccttt ttattggttt atattcaatt
     5101 gtgtatgcat tcacgtagaa atgttctgtt aaacaattaa ttataattat atcggaaaac
     5161 tttcaacacg gtcaaccttt caaatgataa agtaaaatac tacctgcaat cgctacattt
     5221 aaactttcag ctttaccata aataggtatg attaaatttt gtgtagtatg tgctaataat
     5281 tcaggattaa caccttctcc ctcattacct aatagtaatg caaaagaatc acttgaagta
     5341 acttctttgt atgccactgc gttttcaagt gctgtaccat aaacaggacc attaaattga
     5401 gtaataaacg tatcgagatc ttgtgtcata actggcaaat ggaaaacact accttgactc
     5461 gctcgcaaca ctttatcttg ataaggatct gtcgtaccct tctccattat tacagcatcc
     5521 attccagcag catccgcagt tctaattaat gtgcctaaat ttccaggatc ttgaacacga
     5581 tcgatgagca atacttgttg tgctttagaa atatcataat gcggcttctc aatgattgcg
     5641 aaaaaccctt gtggtgtcac tgtacctgat aaagattcag caactttcat gtttatttca
     5701 aaaacttctt gcgcgtatgc gataatttgc tgatctaatc ttgccggttc aattgcaaat
     5761 aattgtgtaa ttacaattcc actttgataa gcttcttcaa ttaaatgcac accttcaatt
     5821 aaagctaatc cagttttatc cctctcacgt ttctttttta gcttgttcgc ttgtttaatt
     5881 ctattatttt gtgcagaagt aatttgttcc attgatagct cctcgcttta tttttaaaaa
     5941 taaaaatatt aatcattaat aagatgaaaa catttgattg tatagttaat attaattaat
     6001 cgctttt
//
LOCUS       DAKTLH010000150         5984 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.153, whole genome shotgun sequence.
ACCESSION   DAKTLH010000150 DAKTLH010000000
VERSION     DAKTLH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.153"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            1..348
                     /locus_tag="O0394_002340"
     CDS             1..348
                     /locus_tag="O0394_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500405.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            365..1000
                     /locus_tag="O0394_002341"
     CDS             365..1000
                     /locus_tag="O0394_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCY0500406.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            1017..1826
                     /locus_tag="O0394_002342"
     CDS             1017..1826
                     /locus_tag="O0394_002342"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCY0500407.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            1823..2677
                     /locus_tag="O0394_002343"
     CDS             1823..2677
                     /locus_tag="O0394_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCY0500408.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMTNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDRIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            2698..4083
                     /gene="mgtE"
                     /locus_tag="O0394_002344"
     CDS             2698..4083
                     /gene="mgtE"
                     /locus_tag="O0394_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCY0500409.1"
                     /translation="MSMNTDEKERVQEELYDQTLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            4093..5937
                     /locus_tag="O0394_002345"
     CDS             4093..5937
                     /locus_tag="O0394_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HCY0500410.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
BASE COUNT         2084 a          830 c         1123 g         1947 t
ORIGIN      
        1 atgcgtttat atattaatga aattaaaatt aaagatgaca tactttattg ttatacagaa
       61 gattctatta aaggattatc tgaagtagga caaatgctcg ttgatagtga taattatgcc
      121 tttgcgtata cattagatga tggtaaagcg tatgcttatc tcattttcgt acaagaaaca
      181 tggacgatgt tgcatgaaaa catgactaaa aaaattatta tcaatgatga actagaattg
      241 actgaattcc accaagaact tacttatatt ttagacaaca taaaagggaa taataattat
      301 ggtaaggaat ttgttgcaac cgttgaagaa acattcgaca ttgaataaag cggggtgaag
      361 cactatgaat caatgggatc agttcttaac accttataag caagcggttg atgagttgaa
      421 agtgaaactt aaaggcatgc gcaaacaata tgaagttggt gaacaagcgt cgccaataga
      481 atttgttact ggtcgtgtta aaccaatcgc tagtattata gataaggcaa acaaacgaca
      541 aataccattt gataggttaa gagaagaaat gtacgatatc gctggtttaa gaatgatgtg
      601 ccaatttgtt gaagatattg atgttgtcgt caatatttta agacaaagaa aagattttaa
      661 agtaattgaa gaacgagatt atattcgtaa cactaaagaa agtggttacc gctcgtatca
      721 tgtcattatt gaatatccaa ttgaaacatt acaaggccaa aaatttatat tggctgagat
      781 tcagattcgt acattagcaa tgaatttctg ggcaacgatt gaacatactt tacgatataa
      841 atatgatggt gcttatccgg atgaaattca acatcgtttg gaaagagcgg cagaagcagc
      901 gtatttactt gatgaagaga tgtctgaaat taaagatgaa attcaggaag ctcaaaaata
      961 ttacacgcaa aaacgttcta aaaaacatga aaatgattaa cgaggtgtta taaatcatgc
     1021 gttatacaat tttaactaaa ggtgactcca agtctaatgc cttaaagcat aaaatgatga
     1081 actatatgaa agattttcgc atgattgagg atagtgaaaa tcctgaaatt gttatttcag
     1141 ttggtggtga cggtacatta ctacaagcat tccatcagta tagccacatg ttatcaaaag
     1201 tggcatttgt tggagttcat acaggtcatt taggatttta tgcggattgg ttacctcatg
     1261 aagttgaaaa attaatcatc gaaattaata attcagagtt tcaggtcatt gaatatccat
     1321 tgcttgaaat tattatgaga tacaacgaca acggctatga aacaaggtat ttagcattaa
     1381 atgaagcaac gatgaaaact gaaaatggct caacacttgt tgtggatgtt aacttaagag
     1441 ggaaacactt tgagcgattt agaggcgatg gattatgtgt atcaacacct tcgggttcaa
     1501 cggcttataa caaagcgcta ggtggcgcac tgatacatcc ttcacttgaa gcaatgcaaa
     1561 ttacagaaat tgcctcgata aataatcgtg tgtttagaac ggtaggatca ccacttgtat
     1621 taccaaagca tcatacatgt ttaatatcac cagttaatca tgataccatt agaatgacga
     1681 tagatcatgt tagtatcaaa cataaaaatg ttaattcaat acaataccgt gtagcaaatg
     1741 aaaaagtgag gtttgcacgt tttagaccat tcccattctg gaaacgtgtg cacgattctt
     1801 tcatatcaag tgatgaagaa cgatgaaatt taagtatcat atatcacaac aagaaactgt
     1861 taaaactttt ttagcacgac atgatttttc taagaagaca gtgagcgcca ttaaaaataa
     1921 tggcgcttta attgttaatg atgaaccagt cacagtgcgt aagcaattaa tgacaaatga
     1981 tatattagaa attcatttac cgcgagaaat accgagtgtt aatttaatac cttatgctcg
     2041 taagctagaa gtattgtatg aagatgcttt tatcatcata gttactaaac caaacaatca
     2101 aaattgtacg ccttcgagag aacatcctca tgaaagttta atcgaacaag tactatatca
     2161 ttgtcaggaa catggtgaaa atattaaccc acatattgtt acgcgtctag atcgtaatac
     2221 aactggtatt gtgatattcg ctaaatatgg acatatccat catttatttt ctaaagtaaa
     2281 cttgaaaaaa atatatactt gccttgtata tggtaaaacc catacatctg gtattattga
     2341 agctaatatt agacggtcaa aggataggat tataactaga gaagttgcct cggatggtaa
     2401 atacgctaaa acatcttatg aagtaataaa tcagaatgat aaatacagtt tatgcaaagt
     2461 tcatttgcat acgggacgta cacatcaaat tcgtgtacat tttcaacata ttgggcatcc
     2521 aattgtggga gattctttgt atgatggttt tcatgacaaa attcatggtc aagtactgca
     2581 atgtacgcaa atatattttg ttcatccaat caataagaac aatatttata ttacaattga
     2641 ttataagcaa ttacttaaat tattcaatca actctaattc acacaggggg tgtaagtatg
     2701 tcaatgaaca cagatgaaaa agagcgtgtt caagaggaat tatacgacca aactttatta
     2761 gatcaatatt tagaaaatga tgatattgat caatttagag atgaatttct agcattacac
     2821 acatatgaac aaagtgagta ttttgaagat actaccgatg aaaatagaca aaagattttt
     2881 caatatttat cacctgaaga agttgcaaat ttctttgatc aattagatat tgatgacgat
     2941 gaatatgagt tgctatttga taagatgaat gcgacatacg caagtcacat attagaagaa
     3001 atgtcatacg acaatgcagt agatatttta aatgagttga ctaaaccaaa agttgctagt
     3061 cttttaacat tgatgaataa agatgacgcg aatgaaatca aagcattact tcactatgat
     3121 gaggatacgg ccggcggtat tatgacgacg gagtatttat cacttaaagc gcatacgcct
     3181 gttaaagaag cattattatt ggtcaaagcg caagcaccag acgcagaaac aatatatgtt
     3241 atatttgtcg ttgatgatga tggtaaatta gtaggtgttt tatcgctaag agatttaatt
     3301 gtagctgaaa atgatgctta tattgaagat attatgaatg aacgtgtcat tagtgtgaat
     3361 gtagcagacg accaagaaga tgttgctcaa gttatgagag actatgattt catggctgta
     3421 cctgttatag attaccaaga acatttgctt ggtatcatca cgattgatga tattttagac
     3481 gttatggatg aagaggctag tgaagactac tctcgtttag ccggggtatc agatatcgat
     3541 tcgactaatg attcaatcat taaaacagca ttaaaacgtt taccatggtt gattatttta
     3601 acatttttag gaatgattac tgcgacaatt ttagggagat tcgaaaaaac attagaaaat
     3661 gtagcgctac tcgcagcgtt tattcctatt attagtggta tgtcaggaaa ttcaggtaca
     3721 caatctttag ccgtttcagt tcgtaacatt acgacagggg aaattaatga gcaaagtaaa
     3781 tttagaattg cattaagaga agcaggaagt ggtgtattat cgggtgttgt atgttcaaca
     3841 atattattta caattattgt tgcaatatat catcagccac ttttagcatt aatcgttgca
     3901 ggaagtttaa cttgtgcgat gacggtgggg acgtttgtag gttcgatgat tccattattg
     3961 atgaataaat taaatatcga tccagcagtg gctagtggac catttattac aacaattaat
     4021 gatattatta gtatgttgat ttattttggt ttagctacat catttatggc ttacttaatt
     4081 taaggaggag ttatggagtt tttatcttta gttattgttg ttttagcagc gtttttaact
     4141 ccaataattg tcaatcgatt aaatattaat ttcttgccag ttgttgttgc agaaattttg
     4201 atggggattg tgattggaaa ttcatttcta aatatagtag aaagggattc aattctaaat
     4261 attttatcaa cgttaggctt tatcttttta atgtttttaa gtggtttaga aattgatttt
     4321 aaagctttta aaaaagataa acgcgcacgt caaggacaaa atgatgatga atcctcaatt
     4381 ccagggcatc ttaatctagc gttaactgta tttgcattta ttatgattat ttcgattctt
     4441 ttagcgtatg tatttaaatg gcttggatta gtggatgatg tgttattaat ggtcattatc
     4501 atttcaacta tttccttagg cgtagttgtt ccaactttaa aagaaatgaa tattatgaga
     4561 acaactatag ggcaatttat cctattagta gcagtacttg cggacttagt aactatgatt
     4621 ttattaacgg tctatggcgc aatcaatggt caaggcggca gtacaatatg gttaataggt
     4681 atattagttg ttttcacagc aatttcatat attttaggtg ttcaatttaa aagaatgtca
     4741 tttttacaaa aattgatgga tggtacgacg caaatcggta ttcgtgcggt atttgcatta
     4801 ataatattat tagtagccct agcagaggga gttggcgcag aaaatatatt aggtgcattc
     4861 ttagcaggtg tcgttgtttc attattaaat ccagatgaag aaatggttga aaagttagac
     4921 tcatttggtt atgggttctt tattcctatt ttctttataa tggttggtgt agatttaaac
     4981 ataccttcat taattaaaga accgaaatta ctaattatca taccgatttt aatcgttgca
     5041 tttatcattt caaaattaat tccagtcatg tttattcgac gttggtttga tatgaaaaca
     5101 acgattgcat cagcattttt attaacatca acattatcgc tcgtgatagc tgcagccaaa
     5161 atttcagaaa gattaaatgc tatttcagct gaaacgtcag gtatattaat tttaagcgca
     5221 gtcattacat gtgtattcgt tccgattatt ttcaaaaaac tgtttccagt tccagatgag
     5281 tttaaccgta aaattgaagt tagtttaatt ggtaaaaatc aattaacgat tcctatagcg
     5341 caaaatttaa catctcagtt atatgacgtg acattatatt atcgcaaaga cttgagtgat
     5401 cgtcgtcaat tgtcagatga tatcacgatg atagaaattg ctgattatga acaagatgtt
     5461 ttagaacgac taggtctgtt tgaccgagac atagttgttt gtgctacgaa tgacgatgat
     5521 attaaccgaa aagttgctaa attagccaaa gcacatcaag ttgagcgtgt catttgcaga
     5581 cttgaaagca caacggacga tacagagtta gttgattcag gtattgaaat tttcagtagc
     5641 tacttaagta ataaaatctt attaaaaggt ttaattgaaa cacctaacat gttgaattta
     5701 ttaagtaatg ttgaaacgtc actatatgaa attcaaatgt taaattataa atatgaaaat
     5761 attcaattac gtaatttccc attcggagga gacatcatct tcgtgcgtat tatccgtaat
     5821 aatgagtcga ttgttccgca tggagataca caattgcgat atggagatcg cttaattgtt
     5881 accggtgcta aagaatacgt tgatgaattg aagcaagagt tagaatttta tttttaacaa
     5941 taatgatatt aaaatgcaga cgtattttag tacgacgtaa aatt
//
LOCUS       DAKTLH010000151         5979 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.154, whole genome shotgun sequence.
ACCESSION   DAKTLH010000151 DAKTLH010000000
VERSION     DAKTLH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.154"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(1..764)
                     /locus_tag="O0394_002346"
                     /pseudo
     CDS             complement(1..764)
                     /locus_tag="O0394_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
     gene            complement(777..1316)
                     /locus_tag="O0394_002347"
     CDS             complement(777..1316)
                     /locus_tag="O0394_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500411.1"
                     /translation="MVAYASHLSFLLFSNKKVLKINAYTGTDMAKLDLNSLDDEHVKL
                     LINELKYPETHIDVNELKTIVASRINERQEIISFKLGIKYLLTIKRGNIEKDRFSISI
                     IFKDTYHTLVRIDINGGTHDNPDGTIAPKSHIHIYNDKYDKKDRFAYEINLKDFPDIY
                     NLYNVYMSFLEYNNIKDLE"
     gene            2115..3050
                     /gene="lukE"
                     /locus_tag="O0394_002348"
     CDS             2115..3050
                     /gene="lukE"
                     /locus_tag="O0394_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HCY0500412.1"
                     /translation="MFKKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKR
                     TEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRM
                     IWPFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFN
                     YSKTISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGS
                     AREYFAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRT
                     GIYAERKHNAFVNRNFVVRYEVNWKTHEIKVKGHN"
     gene            3052..4035
                     /gene="lukD"
                     /locus_tag="O0394_002349"
     CDS             3052..4035
                     /gene="lukD"
                     /locus_tag="O0394_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="HCY0500413.1"
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
     gene            4277..4420
                     /locus_tag="O0394_002350"
     CDS             4277..4420
                     /locus_tag="O0394_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HCY0500414.1"
                     /translation="MEKVLDLDVQVKGNNNTNDSAGDERITSHLFCSFGCEKTGSFNS
                     FCC"
     gene            5158..5301
                     /locus_tag="O0394_002351"
     CDS             5158..5301
                     /locus_tag="O0394_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HCY0500415.1"
                     /translation="MEKVLDLDVQVKANNNSNDSAGDERITSHSLCTPGCAKTGSFNS
                     FCC"
     gene            5366..>5979
                     /locus_tag="O0394_002352"
     CDS             5366..>5979
                     /locus_tag="O0394_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HCY0500416.1"
                     /translation="MNTIYEPSSICMIRTPLLSVEFFNLFLNTEQIKYSDLQLNAQMK
                     ESILTTTFNLYCTLQEINFDGDNKKVRDAKESLLKYLIRMSTRPTPFGLLSGINLGHF
                     VNEPTRLKVGNSIQKYVKVDGEWLYKLVSYIESIDEYYQNLKVIWNSKAHIINDRIYL
                     NEQSAIYLNNNKDTSFSIKNSELLVFIKTTVTNNNITFSNLAEK"
BASE COUNT         2234 a          766 c          908 g         2071 t
ORIGIN      
        1 atctaattcg ctataatcta ttgtactaat atagttttca tgaagaagat ttttattatg
       61 ttcatttatt tctttttttg agtcatttaa aataaaaata aactcatttt gcctgtgtct
      121 ttttattttt ttgcttgcat agcatccgtt attttcgact taataatcat agaattatta
      181 gggttagaaa tagcattgat aaatttttct ttttgatttt tcttagcgct aataaaaaag
      241 tcgaaattat gaacaacacc actgcttcct ataataggta aatctcttcc gtaataaatg
      301 ttatgttcat ccaatttgtt tgcaacatct tctgtaaata tgttctgtga cttaggatta
      361 gaaagtaagt acatatcatt aacaaatata aggcactgta ataaattatg tttcgattta
      421 ttaaaatttt taaagttagt ttgaacaaaa atttcgtgag ttttatcgtt atatttaata
      481 ccgtaagctg aaaggtgctc ttcaaaaatc tttttacgtt tttttgattt atttaaagaa
      541 ataccattat tttctaaatc aaatatagta tagccgtcat ctgtcagtgt aatcatgtct
      601 ctggactgat cgtataaagc gtaaattatt aaattatcta aagaattatc tttaaaagga
      661 gtatctattc ttactacatt gtttgataaa ttagaaaact ctaaggtctg attataccaa
      721 tcaagatatt ctttttttaa ttttttagca tcaaatgtag tcatattatc cctcctttat
      781 tcaaggtctt ttatattatt atactctaaa aacgacatat acacattata caagttatag
      841 atgtcgggga aatctttcaa gttaatttca taagcaaacc tatccttttt atcatactta
      901 tcattatata tgtgaatatg acttttcgga gcgattgttc catctggatt atcgtgagta
      961 ccaccgttaa tatctattct aactagggtg tgataggtat ctttgaaaat gattgaaatt
     1021 gaaaacctat ctttttctat gttccctctt tttattgtta ataagtactt tattcctaac
     1081 ttaaaactta ttatttcttg cctttcattt attcgactag caactattgt ttttaattca
     1141 tttacatcga tatgagtttc tggatatttt aattcattta ttaataattt tacgtgctcg
     1201 tcgtcaagac tatttaaatc aagtttagcc atgtctgttc ctgtataggc attaattttt
     1261 aatactttct tatttgaaaa aagcaaaaaa gataggtgag atgcatacgc aaccaaaagt
     1321 gtataatatc aatttactac ctcgcaatat aattttatac aatgtataat aagatttaaa
     1381 gatgtagctc atcaacattt tcatagggga tttttaaaat taagtattga aaaataaaat
     1441 atttatatta taatataaat aagtcaacga ccatgcgtgg acagtaaaac gaagtacatg
     1501 cgaatgtagt agccgtttac tatgagacta ggcattataa ttgaatatca tagtataaat
     1561 tgccatgaga gtagaagctt cagtttatga agacttcgaa atcatggttt tttattttta
     1621 aagtgaaatt ttagttcata gtaaatatat atatgttttt aattagacta aatcatagca
     1681 ttaaattttt ttagatgaag taagcgatat tacgaaatga tatatagaac atttcagtta
     1741 taacagtata cgaagtaatc atcttcgttt aacggacaat agttaataat cagttaattt
     1801 taaattcaat tatagattat ttgtgataat acaaacatta tttttgaaga tttgattaaa
     1861 gtcgttttta tttttacaga gaaaagattg agtatcggta attttatcat attgttcgaa
     1921 tataaaggga actatttaaa ggtaatgaag gttgcgccaa aatgaccggt tttcagcagc
     1981 ataaatgaat aaattgtgtc atttttaata ttttatagca ttttattttt taatgaacag
     2041 ttaattttat aaataagtga aataatctag aataaatttg tattaaaaat ttatatagaa
     2101 agaaagtgaa acttatgttt aagaaaaaaa tgttagctgc aactttgtca gtaggactga
     2161 ttgcaccttt agcatctccg attcaagaat ctagagcaaa tactaatatt gaaaatattg
     2221 gtgatggtgc tgaagtaatc aaacgtacgg aggatgtaag tagtaagaaa tggggcgtta
     2281 ctcaaaatgt ccaattcgac tttgtaaaag ataaaaaata taacaaagac gctttaattg
     2341 ttaaaatgca aggttttatt aattccagaa cttcattttc agatgtgaag ggtagtggat
     2401 atgaattaac taaacgaatg atttggccat tccaatataa tataggactg acgactaaag
     2461 atccaaatgt tagcttaatc aattaccttc ctaaaaacaa aatagaaact actgatgttg
     2521 gtcaaacatt aggatataac attggaggta atttccagtc agcaccatct ataggtggca
     2581 atggctcatt taattattct aaaacaatta gttataccca aaagagttat gtcagtgaag
     2641 tagacaagca aaactcaaaa tctgttaaat ggggtgttaa agcaaacgaa tttgttacgc
     2701 ctgatggaaa aaaatctgcg catgatagat atttattcgt acaaagtcca aatggtccaa
     2761 caggttcagc aagagaatat tttgctcctg ataatcaatt gccaccttta gttcaaagtg
     2821 gctttaatcc atcgtttatc actacactat cacatgaaaa aggttcaagt gatacgagtg
     2881 aatttgaaat ttcatatggt agaaacttag atattacata tgcgacttta ttccctagaa
     2941 ctggtattta cgcagaaaga aagcataatg catttgtaaa tagaaacttt gtagttagat
     3001 atgaagttaa ttggaaaaca cacgaaatta aagtgaaagg acataattaa tatgaaaatg
     3061 aaaaaattag tcaaatcatc agttgcttca tcaattgcac tgcttttgct atcgaataca
     3121 gttgatgcag ctcaacatat cacacctgta agcgagaaaa aagtagatga caaaatcact
     3181 ttatacaaaa caacagcaac atctgataat gataaattga atatttctca aattttaacg
     3241 tttaatttca ttaaggataa aagttatgac aaagatacgt tagtacttaa ggcagccgga
     3301 aacattaatt caggttataa aaagcctaat ccaaaagatt acaattactc acagttttat
     3361 tggggcggta agtataatgt ttcggttagt tcagaatcaa atgatgctgt aaatgttgtt
     3421 gactatgcac ctaaaaatca aaatgaagaa ttccaagttc aacaaacatt aggttattct
     3481 tatggcggag atattaatat atctaatggc ttatcaggtg gattaaatgg atcaaaatca
     3541 ttttcagaaa cgataaatta taaacaagaa agttacagaa ctacgattga tagaaaaaca
     3601 aatcataaat caattggctg gggtgttgag gcgcacaaaa ttatgaataa tggttgggga
     3661 ccatatggta gagatagtta tgacccaaca tatggtaatg aactgttttt aggcggtaga
     3721 caaagtagtt caaatgctgg tcaaaatttc ttgccaacac atcaaatgcc tttattggcg
     3781 cgtggtaact ttaacccaga atttataagc gtactttctc ataaacaaaa tgatacaaaa
     3841 aaatctaaaa tcaaagtaac ttaccaaaga gaaatggata gatatactaa tcaatggaat
     3901 cgactacact gggttggtaa taactacaaa aatcaaaata cagtaacgtt tacatctact
     3961 tatgaagttg actggcaaaa ccatactgtt aaattaatcg gtacggattc taaagaaact
     4021 aatcctggag tataactgtt agaaatagag gaagttaaac ccgaaattta agagttaaac
     4081 aaaaaatatt cacaacatat taaaaaaaga agttacatta tttgtttaaa ctaacaatag
     4141 taccataaaa ttaatgacga tttacaaatt aataacaaat aattattatt aaattcattg
     4201 atttattgtg tggtaatgat taatatgaca attgaagtgg gtcttaactt caacttttaa
     4261 atagggggtt ttatctttgg aaaaagttct tgatttagac gtgcaagtta aaggaaataa
     4321 caacactaat gattcagcgg gtgacgaaag aataactagc catctttttt gtagctttgg
     4381 ttgtgaaaag acgggtagtt ttaacagctt ctgttgttaa ggatacagca aatagagcct
     4441 gaaagggctc tatttgtctt tttaaatata ttaagtgcta aaaaacggaa acagtttatt
     4501 aatcgccagg tatttttata attagccttc tatattatca gttgagtgtt tcaataaatt
     4561 ctgactttgg tgatggaaag ttatactttt agttgtaata tagttttcat gacttcctgt
     4621 taaataagtt aggattatgt aattagcctc tatctttaca tataagtaat gacaagcttt
     4681 ggttattata ttgaccctct tagaacatgt catttaagaa aataatcaag tcattgaaat
     4741 tatatgttta tatcctcatc tttaaaataa cttcaattat tatttgctaa attttgtata
     4801 aatagaacaa aactgcataa atatctatta attaatctca agcagccatc tccaaattcg
     4861 taatagtatt gatagataaa aatatctaag tatttgtata aatgaattga ttaatctatt
     4921 aaaaaattca tcaataaatt taaaattatg aacttagatg caaaattatt agtgaaaata
     4981 aatagtaata aaaaaaacac aaaaatctaa ttatcaaaat agtattacta aattaataag
     5041 tgtttacaaa aaattcacaa aatataatta tataatttat tgatttgttg tgtgaaaagg
     5101 gataatatga caattgaagt aggccttaac ttcaactaat aataggaggt tttatctatg
     5161 gaaaaagttc ttgatttaga cgtgcaagtt aaagcaaaca ataactcaaa tgattcagca
     5221 ggtgacgaac gtattacaag tcatagttta tgtactcctg gttgtgctaa gactggtagt
     5281 tttaatagct tctgctgtta agattacagc aaatagagcc tgaaagggct ctatttgtct
     5341 attcaaatat gtgaggtgct tacaaatgaa cactatttac gaaccgtcaa gtatttgtat
     5401 gattagaaca cctttattat cagttgaatt tttcaattta tttttaaata ctgaacaaat
     5461 taaatatagc gacttacaat taaatgctca gatgaaagaa tcaatcctaa caacaacatt
     5521 taacttatat tgtactttac aagaaataaa ttttgatggc gataacaaga aggttagaga
     5581 tgctaaagaa agtttattaa aatatttaat tagaatgtca acacgtccaa ctccatttgg
     5641 attattaagt ggtattaatt tggggcattt tgtaaatgaa ccaacacgtc ttaaagttgg
     5701 aaatagtatt caaaaatatg tgaaagtcga tggcgaatgg ttgtataaac tggttagtta
     5761 tattgagagt attgatgaat attatcaaaa tttgaaagtt atttggaata gtaaagcaca
     5821 tattatcaat gatcgaatat atttaaatga acaaagtgct atttatttaa acaacaataa
     5881 agatacatct ttttccatta aaaatagcga attactcgta ttcatcaaaa ccactgtgac
     5941 gaataataat attacttttt caaatttagc tgaaaagat
//
LOCUS       DAKTLH010000152         5871 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.155, whole genome shotgun sequence.
ACCESSION   DAKTLH010000152 DAKTLH010000000
VERSION     DAKTLH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.155"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            28..735
                     /gene="splA"
                     /locus_tag="O0394_002353"
     CDS             28..735
                     /gene="splA"
                     /locus_tag="O0394_002353"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplA"
                     /protein_id="HCY0500417.1"
                     /translation="MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITD
                     ATKEPYNSVVAFVGGTGVVVGKNTIVTNKHIAKSNDIFKNRVSAHHSSKGKGGGNYDV
                     KDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKVKDRISVIGYPKGAQTK
                     YKMFESTGTINHISGTFMEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFG
                     VYFTPQLKEFIQNNIEK"
     gene            860..1582
                     /gene="splB"
                     /locus_tag="O0394_002354"
     CDS             860..1582
                     /gene="splB"
                     /locus_tag="O0394_002354"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplB"
                     /protein_id="HCY0500418.1"
                     /translation="MNKNVVIKSLAALTILTSVTGIGTTLVEEVQQTAKAENNVTKVK
                     DTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSI
                     KKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
                     YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDN
                     RNAYGVYFTPEIKKFIAENIDK"
     gene            1640..2359
                     /gene="splC"
                     /locus_tag="O0394_002355"
     CDS             1640..2359
                     /gene="splC"
                     /locus_tag="O0394_002355"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HCY0500419.1"
                     /translation="MNKNIVIKSMAALAILTSVTGINAAVVEETQQIANAEKNVTQVK
                     DTNIFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKI
                     KSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQAFNFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            2480..3199
                     /gene="splD"
                     /locus_tag="O0394_002356"
     CDS             2480..3199
                     /gene="splD"
                     /locus_tag="O0394_002356"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplD"
                     /protein_id="HCY0500420.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTVVDGIQQTAKAENSVKLIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVTSDAVVQPGSSGSPILNSKREAIGVMYASDKPTGEST
                     RSFAVYFSPEIKKFIADNLDK"
     gene            complement(3148..3255)
                     /locus_tag="O0394_002357"
     CDS             complement(3148..3255)
                     /locus_tag="O0394_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500421.1"
                     /translation="MLILLLISKNHLSMYGWMIYLSKLSAINFLISGEK"
     gene            3357..4073
                     /gene="splE"
                     /locus_tag="O0394_002358"
     CDS             3357..4073
                     /gene="splE"
                     /locus_tag="O0394_002358"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplE"
                     /protein_id="HCY0500422.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIK
                     NTNVAPYNGVVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKV
                     KKIVRYSGQEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYI
                     NKFQMYESTGKVLSVKGNMIITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTK
                     GYGVYFSPEIKKFIADNTDK"
     gene            4224..4943
                     /gene="splF"
                     /locus_tag="O0394_002359"
     CDS             4224..4943
                     /gene="splF"
                     /locus_tag="O0394_002359"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HCY0500423.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTMVEGIQQTAKAENTVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGEST
                     RGFAVYFSPEIKKFIADNLDK"
     gene            5008..5142
                     /locus_tag="O0394_002360"
     CDS             5008..5142
                     /locus_tag="O0394_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500424.1"
                     /translation="MHYLICIKANQRLIFTNSIVDWFYLCMNEQLFDIIKNINDFESI
                     "
     gene            5306..>5871
                     /locus_tag="O0394_002361"
     CDS             5306..>5871
                     /locus_tag="O0394_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCY0500425.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFL"
BASE COUNT         2284 a          862 c         1044 g         1681 t
ORIGIN      
        1 aaaaataaat ttggaaggag gtaaaatatg aataaaaatg taatggttaa aggtttaact
       61 gctttaacta ttttaacatc tcttggtttt gctgaaaata tttctaatca gcctcattca
      121 attgccaaag cagaaaagaa tgtcaaagaa attaccgatg caactaagga accatacaat
      181 tcagtggtag catttgtggg tggtactggt gtagttgttg gtaaaaatac aatcgtaact
      241 aacaaacata tcgctaaaag taatgatatt tttaaaaata gagtatcagc acatcattcg
      301 agtaaaggta aaggcggagg aaactacgac gttaaagaca ttgtagaata tcccggaaaa
      361 gaagaccttg cgatagttca tgttcatgaa acaagtacag aaggtttgaa ttttaataag
      421 aacgttagtt atacaaaatt tgcagacgga gcaaaagtga aagatagaat ttctgttatt
      481 ggttatccaa agggtgcaca aacaaaatat aaaatgtttg aatcgacagg aacgattaac
      541 catatcagtg gaacgtttat ggaatttgat gcgtatgcac aaccaggtaa ttcaggatct
      601 cctgtattga attctaaaca tgaactgatt ggtattttat atgcaggtag tggaaaagat
      661 gaatctgaaa agaatttcgg tgtttatttc acaccacaat taaaagaatt tattcaaaat
      721 aatattgaaa aataagtatc atcaattcat tcgtgaagtt gattttttaa agagtgatta
      781 agaaaacggt tacataatta actaaatata ctgaattatg tatctagata tcaaaataat
      841 taaaagagag gaacttaaaa tgaacaaaaa cgtagtcatc aagagtttag cagcattaac
      901 aattttaaca tctgtaacag gtattggaac aacattggtt gaggaagtac aacaaactgc
      961 caaagcagaa aataatgtca caaaagttaa agatactaat atttttccat atactggtgt
     1021 agttgctttt aaaagtgcaa ctggatttgt agttggaaag aatactattt taacaaataa
     1081 acatgtgtcg aaaaattaca aagtgggcga tcgtattact gcacatccaa atagtgataa
     1141 aggtaatggt ggtatttatt cgattaaaaa gattattaat tatccaggta aagaagatgt
     1201 atcagtcatt caagttgaag agcgtgcaat agaacgtgga ccaaaaggct ttaattttaa
     1261 tgataatgta acgccattca aatatgcggc aggggctaaa gctggtgagc gaattaaagt
     1321 gattggttat ccacacccat acaaaaataa atatgtttta tatgagtcaa ctggccctgt
     1381 gatgtcagta gaaggtagca gtattgtata ttcagcgcat actgaaagcg gaaactctgg
     1441 atcacctgta ttaaacagca acaacgaatt agttggtatt cattttgctt ctgatgtaaa
     1501 aaatgatgat aacagaaatg catatggcgt ctactttaca ccagaaatta aaaaattcat
     1561 tgcagaaaac atagataaat aaacaaattg actttaaacg agcgttgcaa catatctcga
     1621 attgtaaagg agcttgaaaa tgaataaaaa tatagtcatt aaaagcatgg cagcattagc
     1681 cattctaacc tcagtaactg gaataaatgc tgcagtcgtt gaagagacac aacaaatagc
     1741 aaatgcagag aagaatgtta cgcaagttaa agatacaaat atttttccat ataatggcgt
     1801 cgtttcattt aaagatgcga caggttttgt aattggaaaa aatacaatta tcaccaataa
     1861 acatgtatca aaagattata aagttggcga tagaattact gcccatccaa acggtgacaa
     1921 aggaaatggt ggtatatata aaattaaaag catttctgat tatccgggtg atgaagacat
     1981 ctctgtcatg aatattgaag aacaagcagt cgaacgtgga ccaaaaggct ttaattttaa
     2041 tgaaaatgtc caagcattca attttgcgaa agatgctaaa gttgatgaca aaattaaagt
     2101 tattggttac ccattacctg ctcaaaatag ttttaaacag tttgaatcta caggaactat
     2161 aaaaagaatc aaagacaata ttttaaattt tgatgcatac attgaacccg ggaattcagg
     2221 atcaccagtt ctaaattcta acaatgaggt cataggtgtg gtgtatggcg gtattggaaa
     2281 aattggttct gaatataatg gtgccgtata ctttacgcct caaatcaaag attttattca
     2341 aaagcacatt gaacaataaa caaatttaaa tatacaccat gagcatgtgt tcaataattt
     2401 taatgaaaaa catcggtcga atataacata aaaaaacgtc tatatcaaaa gcatcatgaa
     2461 taaacagagg agcacaaaaa tgaataaaaa tataatcatc aaaagtattg cggcattgac
     2521 gattttaaca tcaataactg gtgtcggcac aacagtggtt gatggtattc aacaaacagc
     2581 caaagcagaa aatagtgtga aattaattac caacacgaat gttgcaccat acagtggtgt
     2641 tacatggatg ggcgctggaa caggatttgt agttgggaat catacaatca ttaccaataa
     2701 acatgttact tatcacatga aagtcggtga tgaaatcaaa gcacatccta atggttttta
     2761 taataacggt ggtggacttt ataaagttac taagattgta gattatcctg gtaaagaaga
     2821 tattgcggtc gtacaagttg aagaaaaatc aacgcaacca aaaggtagaa aattcaaaga
     2881 tttcactagc aaatttaata tagcatcaga agctaaagaa aatgaaccta tatcagtcat
     2941 tggttatcca aatcctaatg gaaataaact acaaatgtat gaatcaactg gtaaagtact
     3001 atcagtgaat ggaaatatag tgacatctga tgcggttgtc caacctggca gctctggttc
     3061 acctatatta aatagtaagc gagaagcaat tggtgttatg tatgctagtg ataaaccaac
     3121 aggtgaaagt acaaggtcat ttgctgttta tttctctcct gaaattaaga aatttattgc
     3181 agataattta gataaataaa tcatccatcc atacattgat aaatgatttt tagaaattaa
     3241 caacaaaatc aacaatttta aacatctctg tgattctatt tattcgaaat gatttaaaaa
     3301 ataaaacttc aaaaacctaa ccttatattt atacgaatac ttagaggagc acaaaaatga
     3361 ataaaaatat aatcatcaaa agtattgcag cattgacgat tttaacatca gtgactggcg
     3421 tcggcacaac agtggttgag ggtattcaac aaacggctaa agctgaacat aatgtgaaac
     3481 taatcaaaaa tactaatgta gcaccataca atggtgtcgt ttcgatagga tctggaacag
     3541 gtttcattgt cggtaaaaat acaattgtta ccaacaagca tgtcgttgca ggtatggaaa
     3601 ttggtgcaca tattatagcg catcccaatg gtgaatataa taatggcgga ttttataaag
     3661 ttaaaaaaat tgtccgttat tcaggtcaag aagatattgc cattctacat gtggaagata
     3721 aagctgttca tccaaaaaac aggaatttta aagattacac aggcatttta aaaatagcat
     3781 cagaagctaa agaaaatgaa cgcatttcaa ttgttggcta tccagaacca tatataaata
     3841 aatttcaaat gtatgagtca acaggaaaag tgctgtcagt taaaggcaac atgattatta
     3901 ctgatgcttt cgtagaacca ggcaactcag gttcagctgt atttaacagt aaatacgaag
     3961 ttgtaggtgt tcactttggt ggaaacggcc ctggaaataa aagtacaaaa ggatatggtg
     4021 tttatttctc tcctgaaatt aagaaattca ttgcagataa cacagataaa taaatcctta
     4081 catagataaa tgattttaaa aattaacaac aaactcaaca attcaaatca tctctgtgat
     4141 tccatttatt cgaaatgatt aaaaaaaata aaacttcaaa aagctaacat tataattata
     4201 caaatactta gaggagcaga aaaatgaata aaaatataat catcaaaagt attgcagcat
     4261 tgacgatttt aacatcaata actggtgtcg gcacaacaat ggttgaaggt attcaacaaa
     4321 cagccaaagc cgaaaatact gttaaacaaa ttacaaatac aaatgttgca ccatacagtg
     4381 gtgttacatg gatgggcgct ggaacaggat ttgtagttgg aaatcataca atcattacca
     4441 ataaacatgt tacctatcac atgaaagtcg gtgatgaaat caaagcacat cctaatggtt
     4501 tttataataa cggtggtgga ctttataaag ttactaagat tgtagattat cctggtaaag
     4561 aagatattgc ggttgtacaa gttgaagaaa aatcaacaca accaaaaggt agaaaattca
     4621 aagatttcac tagtaaattt aatatagcat cagaagctaa agaaaatgaa cctatatcag
     4681 tcattggtta tccaaatcct aatggaaata aactacaaat gtatgaatca actggtaaag
     4741 tattatcagt gaatgggaat atagtgtcat cggatgcaat tattcagcct ggtagctctg
     4801 gttcacctat attaaatagt aaacacgaag ctattggtgt aatctatgcc ggtaataagc
     4861 catcaggtga aagcacaaga ggatttgctg tttatttctc tcctgaaatt aagaaattca
     4921 ttgcagataa tttagataaa taattaaaac ttagacattc acccaatcct gacaaaatat
     4981 actataacta acatttatta atatatattg cattatttaa tatgcatcaa agccaatcaa
     5041 cgattgattt tcaccaactc aattgttgat tggttttatt tatgtatgaa tgaacaactt
     5101 tttgacatca ttaagaatat aaatgatttt gaaagcattt gaaagctaca acatttctat
     5161 aaaatttttc aataacaatt gcgccactaa aactcaaaat ttccaccacc aacatccaaa
     5221 ttatcaacat cgcaacataa ccaaatgtta taataaatct attacacaaa gagataaatt
     5281 acttatgcaa aggcggagga atcacatgtc tattactgaa aaacaacgtc agcaacaagc
     5341 tgaattacat aaaaaattat ggtcgattgc gaatgattta agagggaaca tggatgcgag
     5401 tgaattccgt aattacattt taggcttgat tttctatcgc ttcttatctg aaaaagccga
     5461 acaagaatat gcagatgcct tgtcaggtga agacatcacg tatcaagaag catgggcaga
     5521 tgaagaatat cgtgaagact taaaagcaga attaattgat caagtcggtt acttcattga
     5581 gccacaagat ttattcagtg cgatgattcg tgaaattgaa acgcaagatt tcgatataga
     5641 acatctagcg acggcgattc gcaaagttga aacatctaca ttaggtgaag aaagtgaaaa
     5701 tgactttatc gggctgttca gcgatatgga tttgagttca acgcgactag gtaacaatgt
     5761 caaagaacgt actgctttaa tctctaaagt catggttaat cttgacgact taccattcgt
     5821 tcacagtgac atggaaattg atatgttagg tgatgcatat gaattcctaa t
//
LOCUS       DAKTLH010000153         5645 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.157, whole genome shotgun sequence.
ACCESSION   DAKTLH010000153 DAKTLH010000000
VERSION     DAKTLH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.157"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..1258
                     /locus_tag="O0394_002362"
     CDS             <1..1258
                     /locus_tag="O0394_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HCY0500426.1"
                     /translation="IQQLGRGLRKSSNKEYVTVIDFIGNYKTNYLIPIALSGDQSQNK
                     DNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLILKAYEEVENRLGHM
                     PLLMDFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDTLLTENESKNLVFFSRQIAPGLK
                     RIDSLVLEELLKNELTYDELKNKMLNEVKDITEDDIDTSLRILDFSFYNAGIEKIYGS
                     PIIERNERMIRLSDAFTNALSNQTFNMFLEDLIELSKYNNEKYQKGKNGLILYNKYSR
                     EDFSKIFNWNKNGSSVIMGYMIKSQEMPIFITYDKHEDISDSTKYEDEFLSQDELKWF
                     TKSNRTLESKEVQKILSHRAKGIKMYIFVQKKDDDGIYFYYLGTAGYIEGSEKQDKMP
                     NGSNVVTMDLALDKAVRDDIYRYLTN"
     gene            1564..2337
                     /locus_tag="O0394_002363"
     CDS             1564..2337
                     /locus_tag="O0394_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0500427.1"
                     /translation="MIHSKRLRLWLYLVLLAVFIGACGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDQEFEKGDKGTWIIHSKMTIETNGKNMESRGLVLYVDRNT
                     RTTKGEFIVRELWEDKKGYSRSKEKEYPVKMEHNKIIPTKPIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLSNDDYNVKQLRKRYDIPTKKAPKLLIK
                     GDGDLKGSSIGHKNLEFTFIENKEENIYFTDSINFKPTE"
     gene            2449..3233
                     /locus_tag="O0394_002364"
                     /pseudo
     CDS             2449..3233
                     /locus_tag="O0394_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            3230..4554
                     /locus_tag="O0394_002365"
                     /pseudo
     CDS             3230..4554
                     /locus_tag="O0394_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4554..4880
                     /locus_tag="O0394_002366"
     CDS             4554..4880
                     /locus_tag="O0394_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500428.1"
                     /translation="MFTTYKNINELENAYDEERKQLNDAFNQIDELRHQTRKKCEQMY
                     DHFLYLKHKMNYSEDAMIRMTRIIESFDRETNQRIRHHEMKLEDYKDELRREYLKQSD
                     RIEGDE"
     gene            4883..5170
                     /locus_tag="O0394_002367"
     CDS             4883..5170
                     /locus_tag="O0394_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500429.1"
                     /translation="MGLDFSGLPDLAVLEQMKEKEQISEVIAPEHVRMHHDHQNKLKS
                     DEKILLDQMVSHFKKFEDDFKNAAQGAWVKNATDELKDISNDLEKIQDIKV"
     gene            5487..5594
                     /locus_tag="O0394_002368"
                     /pseudo
     CDS             5487..5594
                     /locus_tag="O0394_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
BASE COUNT         2334 a          667 c          995 g         1649 t
ORIGIN      
        1 cattcagcaa cttggtagag gattaagaaa aagttcaaat aaagagtatg ttacagtaat
       61 tgattttatt ggtaattata agacaaatta tttaattccg attgcgcttt ctggggatca
      121 atcgcaaaat aaagataatt ataagaagtt tttaacgaat aacgattcga ttaatggagt
      181 atctacaatt aattttgaag aagttgctaa aaaacagatt tacaattcat tggatgcagt
      241 atcattaaat caaaataaat taatattaaa agcttatgaa gaagttgaaa atagattggg
      301 acacatgccg ttactaatgg atttcataca acaacattct atagatccaa gcgttatatt
      361 ttctaaattt agtaattatt acgagttctt agtgagatat aaaaaaatag atacattatt
      421 gacggaaaat gaatctaaaa atctggtttt cttttcaagg caaatagcac ctggattgaa
      481 aagaatcgat agtttagttt tagaagaact tttaaaaaat gaattaacat atgatgaatt
      541 aaaaaataaa atgttgaacg aagttaagga tataacagaa gatgatatag atacttcatt
      601 aagaatttta gatttttcat tttacaacgc aggaattgaa aaaatctatg gaagtcccat
      661 tatagaacgt aatgaaagaa tgataagact aagcgatgca tttactaatg ctctaagtaa
      721 tcaaacattt aatatgtttt tagaagatct tattgaatta tcaaagtata acaatgaaaa
      781 ataccaaaaa ggtaaaaatg gactgatttt atataataaa tattcaagag aagattttag
      841 taaaatattt aattggaata aaaatggttc gtctgtaatc atgggataca tgataaaaag
      901 ccaagaaatg ccaatattta tcacttatga taagcatgag gatataagtg atagcacaaa
      961 atatgaagat gaatttttaa gtcaagatga attaaaatgg tttactaaat ctaatagaac
     1021 gctagaatca aaagaagttc agaaaatttt gtcccataga gcaaaaggaa ttaaaatgta
     1081 tatttttgta cagaaaaaag atgatgatgg tatatatttt tattatttag gaactgctgg
     1141 atatatagaa ggttcagaaa agcaagataa aatgccgaat ggttcaaacg tagtaacgat
     1201 ggatttagca ctggataaag ctgtaagaga tgatatatat cgttatctaa caaattaatg
     1261 taaaccgaaa aatgaatgtt agtaaagttg tatttaaaca tcaattttaa taagtaaata
     1321 cattgagttt cagtgttatt attttaaata taagcgcaat ttatcttaat taattagttg
     1381 gttaggccaa taaaaattat aaagatattt gcgcttttta aataatttgt gatgttgata
     1441 tgtgaaaaat acaactaaca attaaaatcc acgaggattt aatcataaaa tttcctctaa
     1501 tattattaat attggaattt gaacttaact ttgcatcaaa aataaaaatg gggatgtgaa
     1561 atgatgattc actcaaaaag gttgaggctc tggctgtatt tagtattatt agctgttttt
     1621 ataggggctt gcggaatgaa aaaggaagaa agcagtaaag ataaacaaat taaagaaaac
     1681 ttcaacaaaa cgttgagcct gtatccaact aaaaatcttg aagactttta tgataaggaa
     1741 ggctttcgag atcaagagtt tgaaaagggc gacaaaggca cttggataat tcactctaaa
     1801 atgacaatag aaacaaatgg aaagaatatg gaatccagag gattggtcct gtatgtagac
     1861 cgcaatacca gaacgacaaa aggtgaattt attgtgaggg aattatggga agataaaaaa
     1921 ggatactcac gtagtaaaga aaaagaatat ccagtaaaaa tggaacataa taaaattatc
     1981 ccaaccaaac cgatagctga tgataaatta agaaaagaaa tcgaaaactt taaattcttt
     2041 gtacaatacg gtgactttaa agatattaat gattataaag atggtgacat ttcatataat
     2101 ccgaatgtgc caagttattc agcaaagtat caattaagta atgatgacta caatgtgaag
     2161 caacttagaa agaggtatga tataccaact aagaaagcac ctaaattatt aataaaaggc
     2221 gatggtgatt taaaaggatc atctatagga cacaaaaatt tagagtttac atttatagaa
     2281 aataaagaag aaaacatcta ttttacggat agtattaatt tcaaacctac tgaatagggt
     2341 taatagaagt ttgaaatgaa aatttcataa agtgttcaaa ataatttttc gctttagttg
     2401 tgtaaattaa agattgagtc agtattaaaa atataaggtg gagaaatgat gattcattca
     2461 aagaaattaa cgctcggtat atgtttggta ttactcatta tattgattgg aggttgtgtc
     2521 attatgacaa aaacaaatgg tcgaaacgct caaattaaag aaaatttcaa caaaacatta
     2581 agtgtatatc caaccaagaa tctcgatgat ttttacgata aggaaggttt tcgagatcaa
     2641 gaatttgata aaagagataa agggacttgg attatttatt ctgaaatggt tatcgaacca
     2701 aaagggaaaa atatggaatc gagaggaatg gtgctctata tcaatcgcaa tactagaaca
     2761 acgaagggta attttattgt caccgaaata actgaagata gtaaaggata ttcacgtagt
     2821 aaagaaaaaa atatcctgtc aagatggaaa ataatcgaat tattccaaca aagcctatac
     2881 cggatgacaa gttaaaaaaa gagattgaaa actttaagtt ctttgtacaa tatggaaatt
     2941 ttaaagattt taaagattat aaaaatggtg atatttcata taatcctaat gtgccaagtt
     3001 attctgcaaa gtatcaattg aataatgatg actataatgt tcaacagtta agaaaacgat
     3061 atcatattcc aaccaaacaa gcgcccgaat taaaattgaa aggatccggc aatttaaaag
     3121 gctcatccgt aggatctaag gatctagaat ttacgtttgt agaaaatcaa gaagagaata
     3181 tctatttttc agattcggtc gaatttacac ctagcgagga tgataaatca tgagtcaaac
     3241 ggaatatcaa ataaaatctg gcaatataaa aggtaactct gaagaaacaa gtacagtatc
     3301 taatataagt tatgaaatag aaaacgcaaa taacagtggt ttaaaacaaa ataaaattga
     3361 taaacaaatt aaaaagttac aagaaaaaaa taaattccct aaaaatcttt catatcttaa
     3421 aagttatacg gaccccaaaa caggcacgac tacaagcgcc tttttaaata aagacactgg
     3481 caaagttact ttaggtatga caggtactaa tgtacacaaa gacgcaatat taaaacaaac
     3541 atttggtgtt ccttcttatc aaggatatat agatgtgagt gaaacgctaa aggatattgg
     3601 ggccgatgtc aatattggcc ttcattccgt cacagataaa gatccacatt ataaaaatac
     3661 ccaagacttt atcaaaaata tcaaaaaaga ctatgatatt gatattatta ccggacattc
     3721 gctgggcggt agagatgcga tgattttagg tatgagtaat gatattaaac atatcgttgt
     3781 gtataatcca gctccattag cgatcaaaga tgtgagcggc ctttatgccg atcaagaaga
     3841 acttaaaaaa ttgattgaaa agtacgatgg tcacattgta agatttgtgt ctgatgaaga
     3901 cgaattagat gcaggtgtcc gcaatcattt atatgaaact gctggagaaa aaatagtact
     3961 taaaaatgga gaaggccatg caatgagtgg tattttaatg agcagaacac aggctataat
     4021 cttagctgaa ttaaacaaag ttaaaggcta ccaagacgaa aataataaag cattaaaatc
     4081 cgttcgtaaa caaacgaggc atagattaca taaagtagag acgttaagag cgaattggat
     4141 tcaaacaacg ggtggatcac tctcttcctc ccaacaacaa ttattagaag ctttaacagc
     4201 actaaccatt gccgaaggct taaatcaatt agtgaatgaa gaaagccaac atttgaaaaa
     4261 atgtatcacg cgatggcaca taaatttgga gacaactgga aaaaagcgca agaagttgga
     4321 aatgaaattg gtgaaaaatt aacctctgaa gaggttatag atgaattaag aaaaggtggc
     4381 gcgtatgaaa gtaaacttga aacagatccc aaaagaaaaa ttgatgataa gataaagaaa
     4441 ttaaatgatg tttataaaaa ttgtaatggc tatatcgcaa aaattaaaca gagtatcgaa
     4501 gcaattgttt ctaatgacca aatgttagcg agccagattg atgggatgat gtaatgttta
     4561 ctacgtataa aaatattaat gaacttgaaa atgcctatga tgaagaaaga aaacaattga
     4621 atgatgcatt caatcaaatt gatgaattaa gacatcaaac acgcaagaaa tgtgaacaaa
     4681 tgtatgatca tttcttatat ctcaaacata aaatgaatta tagtgaagac gctatgatca
     4741 ggatgacacg tattatagaa tctttcgata gagaaacgaa tcaacgtatc cgacatcacg
     4801 aaatgaaatt agaagattat aaagatgagt taagaagaga atatctaaaa caatctgaca
     4861 gaattgaagg agatgaataa gcgtgggact tgattttagt ggtttaccag atttagcagt
     4921 attggaacaa atgaaggaaa aagaacagat tagtgaggtt attgcgcctg aacatgttcg
     4981 tatgcatcat gatcatcaaa ataagctgaa aagtgatgag aaaatattac ttgaccaaat
     5041 ggtaagtcat ttcaaaaaat ttgaagatga ttttaaaaat gcggcacaag gggcttgggt
     5101 gaaaaatgcc acagacgaat taaaagatat tagtaatgat ttagaaaaaa ttcaagatat
     5161 taaagtataa aaaggtatta agaaacacaa taagtatata atccatttaa taataaatga
     5221 attatatagt tcataatttc gactataagt ggctattagc cattattttt cgggatctat
     5281 gtcaaatcgg actaatgaat tcaataatgg aagttaagca accaatcttt gtttaacttc
     5341 ttttttattt tttggaaaat aaagttttga acataataaa atttgattat gttttaacga
     5401 attttgatgt ttcttgaact atatatcatc tagtcgtcat ttacaattgg taaatatgac
     5461 ttcaaactgt atgaaggtga tggcgattaa aaggctcatc cgtaggttct aaagaactag
     5521 aatttaactt tataagaaat aaagaagaaa atgtgtattt ttctgatagt attaatttca
     5581 aacctactga atagggttaa tagaagtttg aaataaaaat ttcataaagt gttcaaaata
     5641 atttt
//
LOCUS       DAKTLH010000154         5613 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.158, whole genome shotgun sequence.
ACCESSION   DAKTLH010000154 DAKTLH010000000
VERSION     DAKTLH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.158"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            321..1622
                     /locus_tag="O0394_002369"
     CDS             321..1622
                     /locus_tag="O0394_002369"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCY0500430.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIGGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAINSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIITE"
     gene            1636..1986
                     /locus_tag="O0394_002370"
     CDS             1636..1986
                     /locus_tag="O0394_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500431.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(2138..2608)
                     /locus_tag="O0394_002371"
     CDS             complement(2138..2608)
                     /locus_tag="O0394_002371"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCY0500432.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            2919..4103
                     /locus_tag="O0394_002372"
     CDS             2919..4103
                     /locus_tag="O0394_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCY0500433.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGR
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            4103..5296
                     /locus_tag="O0394_002373"
     CDS             4103..5296
                     /locus_tag="O0394_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HCY0500434.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
BASE COUNT         1948 a          766 c         1131 g         1768 t
ORIGIN      
        1 gttcaaatta tgcaaggttt agaagcagat tcagattact aattttataa aaatatgtaa
       61 gcatgggaca tcgtaaccat atataagtat gtcgatatca gctttaagac atcataaatg
      121 ccatgatgat tctaagataa tttaataggg actttaacgt tataaccaat ctatttaaga
      181 ataataacat cgcattgaaa ttgtgatatt aaaaatgaaa tcgatagctt gctataataa
      241 ttttcgcttg tgcttttaaa aatggactag agaaagcgca gatgctttgt cctaagcttt
      301 ttaagaaaag gatgataaaa atgagaatga tagacattat tgagaaaaag cgtgacggtc
      361 atacactaac aacggaagaa attaatttct ttattggcgg ctatgttaaa ggggatattc
      421 ctgattacca agcatcaagt ttagcaatgg cgatttattt ccaagatatg aatgatgatg
      481 agcgtgcagc attaacgatg gctatggtta attctggtga tatgatagat ttgagtgata
      541 ttaaaggtgt caaagtagat aagcactcaa caggtggtgt aggagataca actacattgg
      601 ttttagcacc attagtagca gctgtagatg ttcctgttgc aaaaatgagt gggcgtggat
      661 taggtcatac aggtggtacg attgataaat tagaagcaat tgatggtttt catgttgaaa
      721 tagatgaagc aacatttgtg aaattggtta atgaaaataa agtggcagtt gtaggacaat
      781 caggaaattt aactcctgca gacaaaaaat tatatgcctt aagggatgtt actggtactg
      841 tcaattcaat accattaatt gcctcttcaa ttatgagtaa aaagattgct gctggtgcag
      901 atgcaattgt attagatgta aaaactggta gcggtgcatt tatgaaaaca ttagaagatg
      961 ctgaagcatt agcgcacgca atggtacgta ttggtaataa tgtgggacgt aatacgatgg
     1021 cgattatttc tgatatgaat cagccacttg gacgtgcgat tggcaatgcg cttgagttac
     1081 aagaagcaat tgatacgttg aaaggacaag gtccgaaaga tttaacagaa cttgtattaa
     1141 cattaggttc tcaaatggtt gtacttgcaa acaaagctga aacgcttgaa gaagcgagag
     1201 cgttgctaat tgaggcaatt aattcaggtg cagcattaga aaaattcaaa acatttatta
     1261 aaaaccaagg tggcgacgaa actgttattg accatccaga gcgtttgcca caagctcaat
     1321 atcaaattga atataaagct aaaaaatcag gttatgtgac tgaattagtc tctaacgata
     1381 taggtgtcgc ttcgatgatg ttaggagcgg gacgtttaac aaaagaggat gatattgatt
     1441 tagcggttgg tattgtttta aataaaaaaa ttggtgataa agtagaagaa ggagaatcat
     1501 tattaacgat tcacagtaat cgccaagatg tagacgatgt tgttaaaaaa ttagattcaa
     1561 gcataacaat tgctgatcat gttgtatcgc ctacattaat tcataaaatc attacagaat
     1621 aggagttgaa gctgattgaa catactaaga tatttattag gaataggttt tagtgtaata
     1681 ggtgtattgc attttacacg agaacgacaa tttagaaata tcataccgaa atgtttgcca
     1741 cttcgaaaaa cagcggttct tgttacagga atctttgaga tattatttgg acttgcactt
     1801 ttaattaaaa gaccatctca atgcttgaaa aatacaatca atctattctt gctagctgta
     1861 ttcccagcta atatctatat ggcagttaaa aagttacctt taggagataa acaattgcca
     1921 acatggcaac tatatttaag gttacctatg cagttcgtgt taatgggact aattaagaaa
     1981 ttatagtggt tctcaaataa atcggacaaa tgttaaagat tttacttttt aatttgttcg
     2041 attttttgac tttgaagcat aataaaaagc agttggacaa tggcgattgt aaaatcattg
     2101 ttcgactgct tttctgatta acttgattga ctaagattta ttttcctgta ccgaaaacat
     2161 catgccattc gtttcgtttg tctagaaatg cttgagcgat agttttagca ccttctaatg
     2221 aatgacttgc tgcccagcca cattgtactt cattacaagc aggcacttca gtagcattta
     2281 gcacatcatt taaagttgct tcaacaatat ttaatacatc atcataatta tcatgattaa
     2341 taaatgatac atagaaacca gtttggcaac ccataggact taaatcaaca actttgtcac
     2401 tatgatttct aatattttca gccattaaat gttctaatga atgtagtccg ggcatatcca
     2461 tatgttcttt gtttggttgt ttgaaacgaa tgtcgtattt gtgaatgaca tctccgttta
     2521 atccttccat cgttcccgct aatctaataa atggggcaac caccttagta tgatctaaat
     2581 tgaaactttc aacattcatt tttgtcattt tgaatttcct cctattaact actcaaaact
     2641 ataaaacgct tatgatttgt tgcattataa aacttaagtt tgttgtaagt tgtgtttcta
     2701 acaatactta aattgtaaca atagggctta tgttttacaa tttgaaactg ttttataaat
     2761 ttatgaaagg cttttagtta ttagaaaatt ttgtaaataa agaaaaacta gatgacatca
     2821 tgagataaaa ccgatagaat aagtaataat tcattttgaa agaacaaaat attgtggatg
     2881 attaaaatga gtgtagggta aaagggagat agtaaaaaat gagtgaaaaa caacaaattc
     2941 tcgattatat agaaacaaat aaatatagtt atatcgaaat cagtcataga attcatgaac
     3001 gtcctgaact tggtaatgaa gaaatatttg cgtctcgaac tttaatagat cgtttgaaag
     3061 agcatgattt tgaaattgaa accgagattg ctgggcatgc aactgggttt atagcgacat
     3121 atgattcagg acttgacggg ccagctatag gttttttagc agaatacgat gctttgccag
     3181 gattgggtca tgcttgtggt cataatatca ttggaactgc aagtgttctt ggtgcaattg
     3241 gtttgaagca agtgattgac caaattggtg gtaaagtagt cgttcttgga tgtccagctg
     3301 aagaaggtgg ggaaaatggt agcgctaaag cttcttatgt caaggctggt gtgattgatc
     3361 aaatagacat tgccttaatg attcatccgg gaaatgaaac ttataaaacg attgatactt
     3421 tggcagtgga tgttttagat gttaaatttt acggaaaaag tgctcatgcc tctgaaaatg
     3481 cagatgaagc gttaaatgca ttagacgcta tgattagtta ttttaatggt gtagcacaac
     3541 tacgacaaca tattaaaaaa gatcaacgtg tgcatggtgt gattttagat ggcgggaaag
     3601 cagctaatat tattccagac tatacacatg ctcgttttta tactagagca atgacgcgta
     3661 aagaattgga tatattaaca gaaaaagtaa atcaaatcgc acgtggagct gcgatacaga
     3721 ctggttgtga ttatgaattt ggtcgaattc aaaacggtgt gaatgaattc attaaaacgc
     3781 cgaaattaga tgatttattt gctaaatatg ctgaagaagt tggtgaagca gttattgatg
     3841 atgattttgg ttatggctct acggatacag ggaacgtaag tcatgttgtg ccaacaatac
     3901 atcctcatat taaaatagga tcacgtaatt tagtaggaca tacgcataga tttagagaag
     3961 cggctgcgag tgtacatggt gatgaagcat taattaaagg cgctaaaata atggcgttga
     4021 tggggttaga gttaattaca aatcaagacg tttatcaaga cattattgaa gagcatgcgc
     4081 atttgaaagg gaatgggaag taatgacaaa taatggtaat gagccgaact taacgttaag
     4141 tgatttatat gacaaagatg ttgtctatac gtcaagacct tcgtatatat cgaatccatg
     4201 gttgaaaccg gacgaacatc aatctaattt tttaactggt agggagttat taatagcaaa
     4261 tcaattacct gttattgttc acgaagctag tgcgacagac aaattgcatc agctatttca
     4321 agttattggt aaggaagtac cgaactcaat ttatacattt aacaatcagc aatcttacga
     4381 aaacttgatc aaacaattgg cacataagga aaataaaaag atttattttc aatatattca
     4441 tgatgaaaca atcttgaatc agcaatatta tgcattagat aaaacgttat ttgttgcttt
     4501 aaataataaa gcaagaatac ctgaatggac aaatgggaag tttttaccaa aaagaaaagt
     4561 tgtaaaaatt gagcaattcg aaaacgagat taagaattgg gaattcccgt tagtaattaa
     4621 gccgggagat gatcttccga cagcaggtgg ctatggcgtt atgatttgtt atcatgatgc
     4681 agatttacaa aaagcgataa cgcgtattaa agaggcaact gctgaaacga attcgcttat
     4741 tatcgaacag aagattgaag aaaaagcgaa ttattgcgtt cagtttgcat actcggaatc
     4801 gctaggcatt caatatcttg gtgcggcaac gcaattaacg gataagtacg gattttataa
     4861 cggaaatgaa aatactacta atgtaccaga gcacgttata gaagcaggta gacaaattat
     4921 ggaaaacggt gtgaatcaag gattctttgg tgtagctggt tttgacctac tcgtcgatga
     4981 ggatgataac gtttatgcga ttgatttaaa ctttagacaa aatggttcaa cgagcatgtt
     5041 attacttgct aacgagttga attcaggata tcaaaagttt tatagttatc attcaaaagg
     5101 tgataacaca catttcttca atacgatttt gaaatatgtc aaagaaggta gtttataccc
     5161 gttatcttat tatgatggtg attggtacgg tgaagataaa gttaaatcaa ggtttggctg
     5221 tatttggcat ggtgattcaa aagaaacagt actggagaat gaacgcgcat ttttagctga
     5281 acttgaacac tattagagtt cggaacataa ggcgctacaa tgttgtgttg ccagtagttg
     5341 actgaatatg cgtttgtaac aagctttttt cgattctagt caacagtaat taaatttatg
     5401 atatggcaat actttgtaat actaatatta aatggcgact tttatttcac tatgttataa
     5461 gagttgccat tttgttgata aaggtatact aaaggttatc gttttgaaat ttttagtaac
     5521 tagatatgtt tcgtgttata gaccgaattt gtgtatacgt aaaatttaat gctattgaat
     5581 ttttaaaatg aaaaacatga cattaaattg aat
//
LOCUS       DAKTLH010000155         5566 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.159, whole genome shotgun sequence.
ACCESSION   DAKTLH010000155 DAKTLH010000000
VERSION     DAKTLH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.159"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(12..359)
                     /gene="sarR"
                     /locus_tag="O0394_002374"
     CDS             complement(12..359)
                     /gene="sarR"
                     /locus_tag="O0394_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HCY0500435.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
     gene            complement(787..1113)
                     /locus_tag="O0394_002375"
     CDS             complement(787..1113)
                     /locus_tag="O0394_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCY0500436.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSANVEMFVEYV
                     KQHMIV"
     gene            complement(1177..1920)
                     /locus_tag="O0394_002376"
     CDS             complement(1177..1920)
                     /locus_tag="O0394_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCY0500437.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDSLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELLRNLKCYLNMCQLSLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVNLNNK"
     gene            complement(1946..3901)
                     /locus_tag="O0394_002377"
     CDS             complement(1946..3901)
                     /locus_tag="O0394_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0500438.1"
                     /translation="MTDNYSLHIYKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDL
                     KRNYVTIIMLNDLYQIDGNDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSAERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPKELNTRYDKEIDHLIDY
                     FILPLRQIEQVSIARSKIICDVDLINENNDLKEKITMSDINKVSELLTNYTKFRGLIM
                     HMSIISESSKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPA
                     ALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKAN
                     FIERNIYDHSLMVSSNSIVHLKYHLEI"
     gene            4511..5314
                     /locus_tag="O0394_002378"
     CDS             4511..5314
                     /locus_tag="O0394_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0500439.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNNYNNGYSYNNYSRYN
                     NYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSGYT
                     SGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQTTQ
                     GAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            complement(5331..5552)
                     /locus_tag="O0394_002379"
     CDS             complement(5331..5552)
                     /locus_tag="O0394_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001795307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500440.1"
                     /translation="MKASIFVSTLTKNLSMYSRFDMPKFGCVNGCMLFILYYRVNRIG
                     ENRNFEIFTNSIFFIEIKKANKALETLLA"
BASE COUNT         1912 a          932 c          671 g         2051 t
ORIGIN      
        1 ttaaccttga tttaattttt aatgtattct tctaattctg aaatcagttt ttgaatattt
       61 gctttttgtg tatctgtaac ataaacaata actgttcttt cgtcttgtaa acttcttttc
      121 tttgataaca attttaaatc ttttagcttt tgtaaagctt tagttaaata gtaaggtttg
      181 aactctgagc acttagcaat ctctttagat gagatttcgt tagactcact tcttaaaata
      241 tgatttaaaa tataaatttc ttcatagttc aaattgaact tcttttttgt atctctgaaa
      301 aacttcttaa cttgaaatgt tgcgttgact aaatcattaa tgtcattaat tttactcatt
      361 attaaaccac tcctctgatg cacatcttgt tgtagcaagg ttcacatgta atttattaaa
      421 tcgaaactct atccaactat gttataaagt tcattctaaa caaataagta taaaaattca
      481 atatttttat tactagaata tggttaaata catttatttc ttataataat ttgatgttta
      541 agatattttg ccaaattgaa aattattagt ctgaatattt tgtatcttaa ctgatatgta
      601 aacttttaaa attttaatct atatcaacgt gtttgatttg atatttttta tattaattgg
      661 atggcttgct cacaaaattt ataaaatata aaaaaagaag agctttaaat cataacgcat
      721 gccatttatc tttaaatgca atgagttatc attcaaactc ttcttatcaa cctacataca
      781 ttgacattat actatcatat gttgttttac atattcaaca aacatttcaa cgttagcact
      841 tttaatatca tgcattggga cattaccaat attaaatttg aacattatag tttgaccatc
      901 ataatctatc ttctctattt cattataact aatacttcta taataaaatt ggccattcat
      961 atctacattc ataattaatc tttcatttgt tgctataaat gccccttcaa attcattctc
     1021 accttgaact tgatactcaa ttattcctag cactgacgga cccttttttt cagttggaaa
     1081 taaatcattt ggattcacat tatctaaaat cataatatcc ctcccactta aaactataaa
     1141 ctattcttca taggtatatg aaatacaagt gattaactat ttattattaa gattaacttg
     1201 tattcccttt tcaagataat tataacatgc ttgaataagc atagtgactt tagaaaactt
     1261 ttctcgcttg attgttacaa taacacgcct ttcatctaat tcacatctct cttttttaac
     1321 taatcccttc aaaattaaac actttaaaat cgggctaacc gcaaatatcg ggtgatggaa
     1381 ttcaccctgt aattctttaa ccgtcaattg acctttatgc agatttaata ttcctaaaac
     1441 gtataactct tctaatgata attgacacat atttaaataa cactttagat tccgcaataa
     1501 ttcttgtgac gacaataaat acgatacata gtcacaacta aaatcaaatg tatattgacc
     1561 aaaataacgt tcaatatagt cactaagctc attattcata tattctattt tatctatatg
     1621 cattttatta atcgaaatag tcaatttacg ttgatccctt tgatctcttc ctttcaatag
     1681 ccattgatga ttgtttaaat aacttaacat ttgaagaagg gccatcttag attggatttc
     1741 tttcattttt aataaactat ctattgaagc gctattggaa tcagaattat catatttaac
     1801 taatagattt aatagtttca cttgagtcaa agtgagatga aatgaatatt tcaacatatc
     1861 attaatgact ttcaacatta gtatgtgtgc agttaaacgc ttaactttaa atttagacat
     1921 ctttaaaacc tctcttaaac catgcctata tctcaagatg atatttcaaa tgaacaatac
     1981 tattgcttga gaccattaat gaatgatcat aaatatttct ttctataaaa ttagctttcc
     2041 aataactgtg ttgttgcata atatcattca caagtacacc attttcggaa gtatgattat
     2101 ctttatctat acttaaaaca atttgtttag ttttagcatg gctaaattgt tgaagaccct
     2161 tacacgataa acgtatagcg tctgaattct catttaacaa tgcggctggc aaacaaatga
     2221 cacattgtac ttcatgtttg aacttcgtta caatcatcgt gtcattttga taaataacaa
     2281 tccctcgtaa ttgattaagt atatttatga taaaaatgaa tattgataat ctagttaaat
     2341 ttatttcttt gctcgattca cttataattg acatgtgcat aataagtcct cgaaattttg
     2401 tataatttgt caaaagttca gaaaccttat taatatcaga cattgtgatt ttttctttta
     2461 agtcattatt ctcattaatc aaatctacat cacatataat tttagaacgt gcaatagaca
     2521 cctgttctat ttgtcttagc ggtaagataa aatagtctat tagatgatca atttctttat
     2581 catagcgcgt attcaactct tttggttcca gatgatatat taattcaaat actcctatat
     2641 aattatctat taaacgtttc agtttttgaa ttgcagtagt atttaagtcg ttaattaaaa
     2701 atgcaatctt acatctatta ctctcaaaag tcatattaaa aattgtagct actagctttt
     2761 ctatcttgcc tctttcagca ctcgttaaca attccttata ttcatgttta atacgaaaca
     2821 ctacatttac attgtaattc actatctttt gaagtaataa agtgatttgt tcaatcgata
     2881 gctcattgct tgtgtcgatt gtaacaatta tatgcaagtt ttcaggatta acacctaatc
     2941 tttgaatgat ttgtttaata gtataataat ccatccaata aaaattactt cctttaatat
     3001 aaatgttttt aggttgatac atttgactcc tttttgtagg ctcaaaaggt atatcaatct
     3061 cgcgcatact tgaagaactt tgattagtat catcaaataa ttcaattata tttttatcaa
     3121 tttcaactaa ttgggaatgg ttaattgaat gagatgttgg actatatctt tttctaatta
     3181 atttaggagt gttaccatac gtttctttaa agaggtgtat aaaacgagaa taatgattaa
     3241 aaccatgact acttgcgatt tcttcaatag gcttcttaga agttaaaata tcaattaagc
     3301 aatgctccag tctaatatga tttaaatatt gaataaaatt actataaggt gtcgctttaa
     3361 acatgtcact taaagctttg tttgtaatac taacttgatt aatgacatct ttcctattta
     3421 tctttttatg gtggttgttt gttaaataat cgtgcacttc ttcggctact aaatgacgac
     3481 taccatcgta tgtatttaat gacattaatt caacacacat gtaattaata atcttatcat
     3541 tagcattata agactgttgt ttaattttgc tgtagattaa atacttaatc aagattctaa
     3601 aagtgctagc aacctcattt gttaatatct tgccacgcat catatagttt gcattcataa
     3661 acttcaaaaa tgtctttgca cttacttcaa caacacaaca tacactatca tcattaccat
     3721 caatttgata caaatcattc aacataataa tggtgacata atttcttttt agatcatatt
     3781 gttgtagatt gattgaaact acacctgtac cttgtaacca atatataatt tttaagttcg
     3841 cttccttcac actactcatt ttcatacttt tataaatatg tagtgaataa ttatctgtca
     3901 tcttacatcc tcacatatac aaatatattg atttactcct gtttgtcttt ctcaaatcaa
     3961 ttgtcaacat ctaatattca ctaccaatta tatcgccaca tgttttttag cagtaacaaa
     4021 acataaatcg attatatcaa ataatacttt tattaaaaca tttttttatg ctaaattagg
     4081 atattttatc cattaaatat acattttcat ccaaatacca aagctttcat aatcatagaa
     4141 acttgatcgt tcgtgtcgaa tttttatgca ttttctgatt aaaagtccta tattatgcac
     4201 aaaatataca tttagaattt tcagtaatat tacaaatttg taacagactt attttcgaaa
     4261 cattcagttt tcacaaagaa tgccgtagtt ataagttttg caaaatgcaa atttctatac
     4321 ttttaaaatt ctattgtgtc aagatattag caaaaaatta caaaacgtta acaaagcaca
     4381 caaggacgct aatttctgtt atagtctttc ttgtcgttta attacgagag ataacttata
     4441 acattaattt tgtcacacgg acattacgaa aatattttag atttaaaatt tttaggagga
     4501 tatttttaaa atgaagaaaa tcgctacagc tactatcgca actgcaggat tcgctacaat
     4561 cgcaattgca tcaggaaatc aagctcatgc ttctgagcaa gataactacg gttataatcc
     4621 aaacgaccca acatcatata gctatactta cactattgat gcacaaggta actaccatta
     4681 cacatggaaa ggtaactggc atccaagtca attaaaccaa gataatggct actacagcta
     4741 ttactactac aatggttaca ataactataa caattacaac aacggttata gctacaataa
     4801 ctacagccgt tacaacaact actcaaataa taatcaatca tataactaca ataactataa
     4861 tagttacaac acaaacagct accgtactgg tggtttaggt gcaagctaca gcacttcaag
     4921 caacaatgtt caagtaacta caactatggc tccatcatca aatggccgtt caatctcaag
     4981 tggttatact tcaggacgta acttatacac ttctggtcaa tgtacatact acgtatttga
     5041 tcgtgtaggt ggtaaaatcg gttcaacttg gggcaatgca agtaactggg ctaacgcagc
     5101 tgcaagagct ggttacacag tgaacaatac accaaaagct ggtgcaatta tgcaaacaac
     5161 tcaaggtgca tacggtcacg ttgcatacgt tgaaagtgtt aacagcaatg gttcagtaag
     5221 agtttcagaa atgaactatg gttatggccc aggtgttgta acttcacgta caatctcagc
     5281 tagccaagct gctggttata acttcattca ctaattagtg atggctatgt ttacgccaac
     5341 aaggtttcaa gagccttgtt ggctttttta atttctatga aaaaaatcga attggtaaaa
     5401 atctcaaaat ttcgattttc accaatccga ttcactctgt aataaagaat aaacaacata
     5461 cagccattga cacacccaaa tttaggcatg tcaaatcggc tatacattga taaattttta
     5521 gttaaagtac ttacaaatat acttgccttc aaattctcaa ttaatt
//
LOCUS       DAKTLH010000156         5507 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.160, whole genome shotgun sequence.
ACCESSION   DAKTLH010000156 DAKTLH010000000
VERSION     DAKTLH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.160"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(1..1085)
                     /gene="sqr"
                     /locus_tag="O0394_002380"
                     /pseudo
     CDS             complement(1..1085)
                     /gene="sqr"
                     /locus_tag="O0394_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II sulfide:quinone oxidoreductase Sqr"
     gene            complement(1104..2438)
                     /gene="cstB"
                     /locus_tag="O0394_002381"
     CDS             complement(1104..2438)
                     /gene="cstB"
                     /locus_tag="O0394_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HCY0500441.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIIIDPVRDLSKYIEVADS
                     EGLTITQATETHIHADFASGIRDVAKRLNANIYVSGEGEDALGYKNMPSKTQFVKHGD
                     IIQVGNVKLEVLHTPGHTPESISFLLTDLGGGSSVPMGLFSGDFIFVGDIGRPDLLEK
                     SVQIKGSTEISAKQMYESVQNIKNLPDYVQIWPGHGAGSPCGKALGAIPISTIGYEKI
                     NNWAFNEIDETKFIESLTSNQPAPPHHFAQMKQVNQFGMNLYQSYDVYPSLDNKRVAF
                     DLRSKEAFHGGHTKGTINIPYNKNFINQIGWYLDFEKDIDLIGDKSTVEKAKHTLQLI
                     GFDKVAGYRLPKSGISTQSVHSADMTGKEEHVLDVRNDEEWNNGHLDQAVNIPHGKLL
                     NENIPFNKEDKIYVHCQSGVRSSIAVGILESKGFENVVNIREGYQDFPESLK"
     gene            complement(2469..3536)
                     /gene="cstA"
                     /locus_tag="O0394_002382"
     CDS             complement(2469..3536)
                     /gene="cstA"
                     /locus_tag="O0394_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide response sulfurtransferase CstA"
                     /protein_id="HCY0500442.1"
                     /translation="MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSI
                     LHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEEENDS
                     YDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSD
                     IKSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAA
                     LIIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFDLMLPKKNIRMPLSK
                     MNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEV
                     EYGGVGTYIGATENANHNLFI"
     gene            3673..3933
                     /gene="cstR"
                     /locus_tag="O0394_002383"
     CDS             3673..3933
                     /gene="cstR"
                     /locus_tag="O0394_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HCY0500443.1"
                     /translation="MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSS
                     LQRLMGIIISENLIECVKAAADDEESSQELINEAVNLLVKSK"
     gene            3933..4688
                     /locus_tag="O0394_002384"
     CDS             3933..4688
                     /locus_tag="O0394_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCY0500444.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANL
                     FDATFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGLFIGIISGIVGAGGA
                     FIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIGSIIF
                     APIGLKLSKKVPNIIQKWIISILIVFAIVQLIL"
     gene            4786..4887
                     /locus_tag="O0394_002385"
     CDS             4786..4887
                     /locus_tag="O0394_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500445.1"
                     /translation="MMKYPYMLININLSTITKNDKRFNATDDRINII"
     gene            4903..5355
                     /locus_tag="O0394_002386"
                     /pseudo
     CDS             4903..5355
                     /locus_tag="O0394_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
BASE COUNT         1747 a          956 c          814 g         1990 t
ORIGIN      
        1 ttagtatttt tattataatc aaactctgca agtattaacc tattatatcc agtaacaata
       61 gggcatgaag tataaccatc ataatgatgc gttaacattt gattattcat cacttgcaat
      121 aaattattag cgacgatagg tgcttgctta cgaatagctg cgcctgtttt tgaagtaggt
      181 acatttgaag catcaccaag tgcaaataca ttagagtagc ttttatgctg taatgtggtt
      241 gggttaacat ctacccaacc ctcactatct gaaagtgtac tttcttttac tacatctaag
      301 ggacccatag gtggtgttac atgtaacata tcataactta ttgtttttct atcgtatgct
      361 ttgatatgtt cgaatgtagc cacttttttg tcaccgtcga tttcaacaag gttataatta
      421 taattgactg ttatatttct ttcttcaaca atcctttcta attctttatt atattttcct
      481 acgtcaaata aagcatcttt tggcgttgca tatatcacat tagcgttaga acggatttta
      541 tgtttcctaa aataatcttc agctaaatac ataattttca taggcgcacc tccacacttt
      601 ataggagtgt ttggatgcgt aaaaatggca tttccttgtt taaaattaga aatttggttc
      661 caagtttcgt taacatagtc aggtgaatag ttagagcaaa caccattttt acctatattt
      721 tcttttagtc ctttaattga agaccaatta atctgtaatc ctggagctac tactaaaaaa
      781 tcataataaa cgactgtatt atctcctaaa ataacgctat tattttcagg ttaaaaactt
      841 gaaacagcct gttttatcca gttagcacct tcaggtataa cactttccat atctttacga
      901 gaacttttca aactagatac ccctgcacca accaacgtcc ataatggttg atagtaatga
      961 tggtctgctg gatctataat tgctattttc tcttttaagt tttgattttt tcttaatagt
     1021 cttgatgcta cggtaacacc tgctgtaccg ccaccaataa taacaatttg ataatgctta
     1081 ttcatttttt ccacatcctt aaattatttt aatgattctg gaaaatcttg atagccttct
     1141 ctaatattca ccacattttc aaaacctttg ctttccaata tacccactgc aattgaactt
     1201 ctaacacctg actgacaatg tacatatatt ttatcctctt tattaaaagg aatattttca
     1261 tttaataatt taccatgcgg aatattaact gcttgatcta agtgtccatt attccactct
     1321 tcatcattac gtacgtctaa tacatgttct tctttacctg tcatatcagc gctatgaacg
     1381 gactgggttg aaatgcctga ttttggcaaa cgatagcctg ctaccttatc aaacccaatt
     1441 aattgtaaag tgtgtttcgc tttctcaaca gtagatttat ctccaattaa atctatatct
     1501 ttttcaaaat ctaagtacca accaatttga ttaataaagt ttttgttgta tggtatattg
     1561 attgttcctt ttgtgtggcc accgtgaaag gcctctttgc tacgaagatc aaatgctact
     1621 ctcttattat ctaaactagg ataaacatca tatgattgat ataaattcat accaaactga
     1681 ttaacttgtt tcatttgtgc aaaatgatgc ggtggtgctg gttgatttga tgttaatgat
     1741 tcaataaatt tagtctcatc aatttcatta aatgcccagt tattaatttt ctcataacct
     1801 attgtagata taggtatggc acctaatgct ttaccacaag ggcttccagc accatgaccc
     1861 ggccagattt gaacatagtc tggtaaattt ttaatatttt gaacggactc atacatttgt
     1921 ttcgcgctaa tttctgtaga accctttatt tgaacagatt tttctaataa atcaggtcta
     1981 cctatatcac caacaaaaat aaagtcacca ctaaataatc ccatcggaac acttgaacca
     2041 ccacctaaat cagtgagtaa aaagctaata ctttcaggcg tgtgtcctgg agtatgcaga
     2101 acttctaatt taacattgcc tacttgaatg atatctccat gtttaacaaa ttgtgttttt
     2161 gatggcatat ttttataccc taatgcatct tcaccttcgc cagacacata tatatttgca
     2221 tttaagcgtt tagccacatc acgaattcct gaagcaaaat cagcatgaat atgtgtttct
     2281 gtagcttgtg taattgttaa accttcagaa tctgcaactt ctatatattt cgataaatca
     2341 cgaacagggt ctattattat tgcctctcct gtacgttgac aacccactaa atatgatgct
     2401 tgagataaat gattatcgta aaactgttta aaaaacatga taacaactcc ttttattaat
     2461 agatttaatt agataaataa attatgattc gcattttcag tagcaccaat ataagtgcct
     2521 acaccaccgt actcaacttc atctctaagt tcttctttct gaatacccat gacatccata
     2581 ctcatcgtac aagcgattaa tttgatattt tgctcaatag cttgattgat aagtgttggt
     2641 aatgaatcaa catttttctt tttcattacg tagcgcatca tcatatttcc taaaccaaac
     2701 atattcattt tggaaagagg cattcgtata ttctttttgg gcaacattaa atcaaacatt
     2761 tttgcaatac cttgcttttt aacattaact gtttgcactt tttttaatgc attaagcccc
     2821 caaaaagtaa agaagatagt tacatctttt ccagcagctc tagcaccatt tgcaataatc
     2881 aacgctgcta cagctttgtc taattctcca ctaaataata caattgtagt acctttagca
     2941 gaataattta tatctaaatc ttttgctttt tctttttgaa taatcgcatt aattccattg
     3001 ttattttcat caagcttaac taaagtatgc cttgtttgtt tcacccaact tttaatgtca
     3061 ctagggaatc cagggtctgt gactttgact tcaatttggt cacctacttc aatattcttc
     3121 atttcttgac taatttttac aattggccct ggacattgaa gaccacgata gttaaattgt
     3181 ttacgatctg ctttaatttc tatactttta tattcttctt gtgtatcata actatcgttt
     3241 tcttcttcat aagctttata accaccatca agatttataa cgttataacc ttgtttagct
     3301 aaatatttac tagcattagc acttctgtta ccacttctac agattacata ataggtttta
     3361 tttttttctt tattgaatga ctcaatctca tcaacaggat gaagtatgga accattgata
     3421 tgtcctaatg catactcctc ttctgttcta acgtcaatta attgcccttg cttggctagt
     3481 ttttccaatt ctgctttact aaattcatcg ataaactttt ctccgtattg tttcataata
     3541 acctccctaa aaatacctgt atgggtattt taaacagttg gatggaactt gtcaaatacc
     3601 tctaggggta tttgacacat ttgtgtttat ctattaatat atttttattc aattaaaagt
     3661 ggaggtatga ctatgaatta tgataaaaaa atgattaatc gtattaatag aatacaaggg
     3721 caactaaatg gaattattaa aatgatggag gaaggaaaag actgtaaaga tgtcattaca
     3781 caaataagtg catcaaagag ttcactccaa cgcttgatgg gtatcattat tagtgagaat
     3841 ttaatagaat gtgtaaaagc agctgcggat gatgaagaaa gctcccaaga gttaattaat
     3901 gaagctgtaa acttattggt gaaaagtaag taatggatat tgttaatata gttatcatgt
     3961 tactcattgg ggtattcggt gggttcatat ctggtctagt aggcgtaggt ggtgctatca
     4021 ttatttatcc agctatttta ttattaccac cattattcgg agcccctgca tatagcgcat
     4081 atatcgcatc aggtcttact tctagccaag tctttttcag tacattaagt ggctcattaa
     4141 aagctcgaaa aaaaacagag ttctcgccac aattagttct ttatatgggt ggcggcatga
     4201 ttatagggag tatgttaggt gcatttctag caaatttatt tgatgctaca tttgtaaata
     4261 cggtatatat cattattgct ttacttgcat taacattaat gtttattaaa gtgaaaccta
     4321 gttccgaaaa gtcttcattt aataaatatt tattagttat cattggcctt ttcattggta
     4381 ttatttcagg tatagtaggt gcaggtggtg ccttcattat tattcctata ttattggttt
     4441 tattcaaatt accaatgaat actgtagtcg ctaatagtat agtaattgct tttatatctt
     4501 caattggtgc ttttgtcatc aaacttattc aaggttatat ccctttatat gacgccttat
     4561 tccttataat agggagtatt atatttgctc caatagggtt aaaattaagt aaaaaagtcc
     4621 ctaacataat acaaaaatgg attatcagta ttttaatcgt ttttgctata gttcaactca
     4681 ttttataaaa ctaatccttt taactcagac agcaatttcc cgaaaaataa tacctcttat
     4741 ttaaacaaat atgtatgcag ttcggattta tataaataaa aatttttgat gaaatatcca
     4801 tatatgctta taaatataaa tttatcaact ataactaaaa atgataaaag atttaacgcc
     4861 actgatgacc gaattaatat tatttaagtt tttatcaaac gcatgagtaa agtagatgca
     4921 cattgggaat taatcgaagc tattaaaaat ttacgtgacg aaattgcacc aaacacattg
     4981 ttaacaatta acgatgatat tcccgataga aaaacaggac ttgaacttgc agaaaaatat
     5041 ggtattgatg gcatcatgat tggtagaggc attttccaca atccattcac ttttgaaaaa
     5101 gaaccacgcg aacacacaag caaagaacta ttagatttgt tgagattgca tctatcattg
     5161 tttaacaaat atgagaaaga tgaaatacga caattcaaga gtttgcgtag attctttaaa
     5221 atctatgtgc gtggcataag aggcgctagc gaacttcgcc atcaattgat gaacacacaa
     5281 tcaattgcag aagtacgagc actactagat gaatttgaag cccaaatgga cgaagacgtt
     5341 aaaattgaat tatagtatga gtaataaggg aaaggaggaa tttaagtttc ctcctttgtt
     5401 tgtgtgaaag tcttttaggt atgaactagg gtgatttaaa ataaaagttt aatttctatc
     5461 ttcagttggc gtttccatat aaataattgg attaattctt tgatctt
//
LOCUS       DAKTLH010000157         5289 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.161, whole genome shotgun sequence.
ACCESSION   DAKTLH010000157 DAKTLH010000000
VERSION     DAKTLH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.161"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..732
                     /locus_tag="O0394_002387"
     CDS             <1..732
                     /locus_tag="O0394_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500446.1"
                     /translation="DCSMYSKVFEQYKKNVVLDKVYYSEVYKYSDNLIKVFSEDVWNI
                     MGYKKYNEYFTNRDDTTLKITNLYIGQSFGTEGNRNVIDRIGKGHEKLQEIMTYQPEN
                     RKTVLIFYKIQPDNIYITNQYGKEAVSRLKRILEQSIPNKEYIDLSEISLITFFKPEY
                     NKQHVNEEFNSLSSSKIKNLAKENDGIIIYLNFKDVNYQLISEGNTFFSAKPYLHCVF
                     NKDILNMDKVDIENFFKSIEVKYSK"
     gene            1286..1468
                     /locus_tag="O0394_002388"
     CDS             1286..1468
                     /locus_tag="O0394_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500447.1"
                     /translation="MKDSIFWKKAFIPVYFIVAMLVFLLFRFYIKTDNFSIYLMSIFL
                     ICLGTASIIYNYKTNR"
     gene            complement(1450..1689)
                     /locus_tag="O0394_002389"
     CDS             complement(1450..1689)
                     /locus_tag="O0394_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500448.1"
                     /translation="MCVVIMRDVGLIDVEDCYDIFGMWCLVNGGLKLSFYGGLKMLFY
                     VGLEFVVLWRYKKPQQDLDLTVTYTFHTYIYRLVL"
     gene            1922..3586
                     /gene="alsS"
                     /locus_tag="O0394_002390"
     CDS             1922..3586
                     /gene="alsS"
                     /locus_tag="O0394_002390"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCY0500449.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            3623..4327
                     /gene="budA"
                     /locus_tag="O0394_002391"
     CDS             3623..4327
                     /gene="budA"
                     /locus_tag="O0394_002391"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCY0500450.1"
                     /translation="MTNVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDKDFTK
                     ANIDYKDIADEIREAE"
     gene            complement(4691..5116)
                     /locus_tag="O0394_002392"
     CDS             complement(4691..5116)
                     /locus_tag="O0394_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCY0500451.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKEMTVDGY
                     HTVPYTISVDGITALHRTYFIFPENKNVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
BASE COUNT         1923 a          743 c          901 g         1722 t
ORIGIN      
        1 gattgtagta tgtactcaaa agtatttgaa caatataaaa aaaacgttgt attggataaa
       61 gtatattact ctgaagttta taaatatagt gataatttga taaaagtatt ctctgaagac
      121 gtttggaaca taatggggta taaaaaatat aatgaatatt ttacaaacag agatgatact
      181 acattaaaaa ttactaattt atatattggg caatcttttg gtacagaggg taatagaaat
      241 gtcattgata gaatagggaa aggtcatgaa aaattacaag aaattatgac ttatcagcca
      301 gaaaatagaa agactgtctt aatattttac aagatacagc ctgacaatat ttatattaca
      361 aaccaatatg gtaaagaggc tgtgtctcga ttaaaaagaa ttttagagca aagtatacct
      421 aataaagaat atattgattt atcagagata tcattaatta cattttttaa accagaatat
      481 aataaacaac atgtgaatga ggagtttaat tctttatctt cttcgaaaat aaagaattta
      541 gccaaagaaa atgatggtat tattatttat ttaaatttta aggatgttaa ttatcaactg
      601 ataagtgaag gcaatacttt tttttctgca aaaccatatt tgcattgtgt gttcaataaa
      661 gatattttaa atatggataa agttgatata gaaaattttt tcaaaagtat agaagtcaaa
      721 tacagtaaat aagatcaatt attgaatcca ccgaccagca gattttttta aaaaattaat
      781 accccgttaa taccctttgc ttcaatttga tgaaaatcaa tgaaattcaa aattgaagaa
      841 atcctttaat atcaaggtgt acgacagtct atataatcat gcgaaattct aaaattttct
      901 gaacgtaaaa aaacaaattc ttaaagcagc tcgtcgttca cctcaattct caaaacgtta
      961 attgtcggac gatatatata caaaacacct cgatgttatg tcgaggtgtt tttttgcgtt
     1021 tgtgtgggga atatggaata gagtataaat gaattaatgt ctcaggtata gaattaattc
     1081 aactatgaat tattgattta cacgtataat ttgtacatgc ttacacaatc aaaagtgtac
     1141 attattaaat tgtcatttag cttgcggaga ccgtacgata gtgttctaat aatagatatt
     1201 caagggaaag agctatcgga tgataaccag atgataactc aagaaccatg atttaagagt
     1261 gttatcaaaa taaggagtga cttaattgaa agattctata ttttggaaga aagcttttat
     1321 tcctgtttat tttattgttg cgatgctggt gtttctactt tttaggtttt atattaaaac
     1381 agataacttt tctatatatt taatgagtat cttcttaatt tgtttaggaa ctgcttctat
     1441 catttataac tataaaacca atcgataaat ataagtatga aatgtataag tcacagttag
     1501 atctaagtct tgctgtggct ttttatatct ccataaaacg acaaattcaa gcccgacata
     1561 aaacagcatt ttcagcccac cataaaacga caatttcagc ccgccattga ctaaacacca
     1621 catcccaaaa atatcgtaac aatcctctac atcaatcaat ccaacatccc tcataatcac
     1681 aacgcacaaa atctattcat gcatttttgg aatacttagt attacaaata acgattttta
     1741 ttcatctaac aaaggttata taatgtactg aaggcaattt ttatgtatta caaatctaat
     1801 cgtacatgta aaattttgat aaacatcatt aattttgcgt aactatcatt agattacaaa
     1861 tcacaaagta attacatgta atacacatct atacatcaca tttgaaggga aatgaatata
     1921 aatgactgat aaaaagtaca ctgcagccga tatggttatt gatactttga aaaataatgg
     1981 ggtagaatat gtttttggta ttccgggtgc aaagattgac tatctattta atgctttaat
     2041 tgatgatggt cctgaactta ttgtcactcg tcatgaacaa aacgccgcaa tgatggcgca
     2101 aggtattgga agattaacag gcaaaccggg tgtagtactt gttacaagtg gtcctggtgt
     2161 aagtaattta actactggat tattaaccgc aacatctgaa ggggatcctg tattagcgtt
     2221 aggtggccaa gtgaaacgta atgatttatt acgattaacg catcaaagta ttgataatgc
     2281 tgcgctatta aaatattcat caaaatatag tgaagaagta caagatcctg aatcattatc
     2341 agaagttatg acaaatgcaa ttcgaattgc tacttcagga aaaaatggcg caagttttat
     2401 tagtattccg caagacgtta tttcttcacc agttgaatct aaagctatat cactttgcca
     2461 aaaaccaaat ttaggagtac cgagtgaaca agatattaat gatgtcattg aagcgattaa
     2521 aaatgcatca tttcctgttt tattagctgg tatgagaagt tcaagtgcag aagaaacaaa
     2581 tgccattcgc aaattagttg agcgcacgaa tttaccagtt gtagaaacat tccaaggtgc
     2641 aggtgtaatt agtcgtgaat tagaaaatca tttcttcggt cgtgtgggct tattccgcaa
     2701 tcaagttggt gatgaattat tacgtaaaag tgatttagtt gttacaatcg gttatgatcc
     2761 aattgaatac gaagctagta actggaataa agaattagaa acacaaatta tcaatattga
     2821 cgaagttcaa gctgaaatta ctaattatat gcaaccgaaa aaagagttga ttggtaatat
     2881 tgctaaaacg attgaaatga tttctgaaaa agtggatgag ccatttataa atcaacaaca
     2941 tttagacgaa ttagaacaat taagaacaca tattgatgaa gaaactggta ttaaagcgac
     3001 gcatgaagaa ggaattctac atccagtgga aattattgaa tctatgcaaa aggtattaac
     3061 tgatgatact actgtaacag ttgatgttgg aagtcactat atttggatgg cacgtaattt
     3121 cagaagttac aatccaagac atttattatt tagcaatggt atgcaaacgc ttggtgtagc
     3181 attaccgtgg gcaatttcag ctgcacttgt gcgccctaat acgcaagttg tgtccgttgc
     3241 tggcgatggt ggctttttat tttcatcaca agatttagaa acggccgtac gtaaaaattt
     3301 aaatatcatc cagcttattt ggaatgatgg aaaatataac atggttgaat tccaagaaga
     3361 aatgaaatat aaacgttcgt caggtgtaga cttcggtcct gtagattttg taaaatatgc
     3421 agaatcattt ggcgcgaaag gtttacgagt tactaatcaa gaagaattag aagcggcaat
     3481 taaagagggc tatgaaacag atggtccagt attaattgat atacctgtaa attacaaaga
     3541 taatatcaaa ctttcaacaa atatgttacc tgacgtattt aactaaaata aagataaatg
     3601 ttaaagagga gtgggagatt ttatgactaa tgttttatac caacatggta cattaggcac
     3661 attaatggca ggattattaa aaggaactgc atcaataaat gaattattgc aacatggtga
     3721 cttaggtatc gctacactaa caggttcaaa cggtgaggta atctttttag atggaaaagc
     3781 ttaccatgca aatgaacata aagaatttgt agaattaaaa ggtgacgagt taacaccata
     3841 tgcaactgta actaaatttg tagcagatac aagctatgaa acgaaagata aatcttcaga
     3901 agcagttttt gcagaaatta aggaaaagat gttgagtgaa aatttatttt cagcagtaaa
     3961 aatttcaggc ttatttaaaa aaatgcatgt acgtatgatg ccggctcaag aaccacctta
     4021 tacacgttta atcgattcag ctagaagaca acctgaacaa actgaaacgt atgtcaaagg
     4081 ttcagtcgtt ggtttcttta caccagaatt attccatggt atcggatcag caggatttca
     4141 tgtacacttt gcgaatgatg atcgtaactt tggtggacat gtcttagatt ttgaagtaga
     4201 agatgttaaa gtagaaatcc aaaatataga aacatttgaa cagcattttc caattcaaga
     4261 taaagatttc actaaagcaa atattgacta taaagatatt gcagacgaaa ttagagaagc
     4321 tgaataatga atccagaaat ataatgacgg tttatgaaaa ttgacttcat aatgcgcgat
     4381 ttagaaatga tagtttgtaa atatgattaa ccatgactac aatagaacaa atatatttat
     4441 aattacgtct aagtaataaa ataaatccct tcactattag cagtagtgag gggatttatt
     4501 aggttccaga tatttgagat ttgctgttat gtttagatta taaattgtgg tacacactca
     4561 tataaaattt actattgtat aggccaatct gttactacga gaagcaaaca acaataattt
     4621 acaagttcaa taactaaaaa gacaaacgcc aatttttcag cgcttgccct ataaaactat
     4681 tttcaaatta ttatttaact acaatttgta tctgtttgat tgtacttgga tcaattgaat
     4741 ttttagcgtc gtcatttttc tttagattca ctactttttt attaccatca ttcaaagtaa
     4801 gcgtataagt tgctgtttgg gcattattaa ttttttctgt tgtaacacca cgttgagaag
     4861 ctaattcatt ttttacttta ctgtcaattt cttgataaag aacattttta ttttctggga
     4921 agataaagta agttcgatgt aatgcagtaa taccatctac tgaaattgtg taaggaacag
     4981 tgtgataacc atccacagtc atttctttat agccgttatt actatctgca gatgcttcgt
     5041 gactcggtaa tgcagctccg accgttgata ataatcccaa tgctaaagtg gcagtaacaa
     5101 atgattttag tttcatatct caatctcctt ttcgggttgt atgatattac actatttgta
     5161 taacacgttt cataaagttt aaaacatact ttttgcattt tttatatttt aatttttagt
     5221 aatagttttt aacgagggat atgactcgtg cattttgaaa agtggtaagc aatgttttta
     5281 gtttatagg
//
LOCUS       DAKTLH010000158         5255 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.162, whole genome shotgun sequence.
ACCESSION   DAKTLH010000158 DAKTLH010000000
VERSION     DAKTLH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.162"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(202..759)
                     /locus_tag="O0394_002393"
     CDS             complement(202..759)
                     /locus_tag="O0394_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCY0500452.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPYTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
     gene            complement(826..2466)
                     /locus_tag="O0394_002394"
     CDS             complement(826..2466)
                     /locus_tag="O0394_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HCY0500453.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQGGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTATKYTDASTYIELLATKLHQAKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNN
                     ATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMAVEDAPKKLIDIAKAFSQQNK"
     gene            2660..2761
                     /locus_tag="O0394_002395"
     CDS             2660..2761
                     /locus_tag="O0394_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500454.1"
                     /translation="MMLQNTIAKKKQARHLMKVYAPVYSKHYLASNN"
     gene            complement(2740..4785)
                     /locus_tag="O0394_002396"
     CDS             complement(2740..4785)
                     /locus_tag="O0394_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCY0500455.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGESLQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
BASE COUNT         1671 a          933 c          940 g         1711 t
ORIGIN      
        1 gtgatacata attttgcaat aaatactaaa ttttttgaaa attccggaat aaatactgtt
       61 ttttatgtag taggggcgta agttaagaac gatttaacag atatagaata tgttaaatat
      121 agaaggttag acaacccgac gtattaatac tttgtgaaat aaagatcccc ctcaacatga
      181 tcgaaggggg gaagtatatg tttagttcaa tatatttaga aagtcgtttg tagttattgt
      241 ttgtcccatc aatgggaata cattatctat tggaaattga tgtagcgttt cgttttgtgc
      301 actcatcata tctgtaacaa aaaactgatt gtagtttaat tgataggcat ctcgcgctgt
      361 cgtatctacg ccaatatgcg ttgcgacacc accaagaaca atcgtatcaa ttcctcgacg
      421 tcgcaattgt aagtccaaat ctgttcctac aaatgcacta aaatgtcgtt tgtctatgac
      481 aaaatcgtca tctctcttgt ctaataaatg atggaaacga ctgtagtcgt cgccttcttt
      541 tggtggtaat gagatcattg catttggttg caatacatct ttaccatcat agaaattcac
      601 gcgaacaaaa gcgataaagc cattgttttt tctaaaaaca tctattaatt tattagcgtt
      661 ttgaacgaca ttttcagctg tatatggggc ataatccatt ttaagaatac cttcttgcag
      721 gtcgattaac actaaagcgg ttttattaaa attaatcatc ttatatcaga gtcctattcg
      781 tttaataaag tattcgctta aacttatacc ctgtatacga tgaaattatt tattttgttg
      841 tgaaaaagct ttagcgatat cgatgagttt cttcggtgcg tcttcgacag ccattttgac
      901 ttcgacaaaa tgcatcacat cgggatgacc attaattgca ttaaacgtgt cttgtaaatc
      961 ttttgatgat tcaacgtcat gaatttcaac atttttacca ccaaatacag ctggtaaagc
     1021 tttataatcc cacatgtgaa tttcattata aggttcatac atgccgtgaa taagtcgttc
     1081 taccgtatag ccgtcattat taatcacaaa taataccggt ttaatatgct gtctaatcat
     1141 agttgaaata gcttgaacag ttagttgcaa tgagccatca ccaattaata ataagttacg
     1201 acgatctttg tctgctaatt gtgaacctaa tgttgcaggt aatgtatagc cgatagaacc
     1261 ccataacggt tgccctataa aagtattgtt tttgtataat gctaaatcat aagcaccaaa
     1321 gaatgatgta ccttgatcag caatgatgac atcatttggt tttaagaaat tttgcatcat
     1381 tttaaaataa gtttgttgtg ttaatggttc tgtgccaaca gtataatcgg gtgatgttgg
     1441 acgatgatac gcagggaacg ttgcgttatt cgtatatgaa atattggata actgttttaa
     1501 caatgatggt agagatattt catcatttgt aacatcgtca attttgatat tgtgatgatt
     1561 taacataacg acatcatcga tattgaattg gtatgaaaaa cctgctgttg ctgaatctgt
     1621 taatttggct ccaatattta aaattaaatc gctgttgtcc acataatctc gtattttatc
     1681 ttcggcaatt ttcccatcgt aaatacccat ataatatgga ttttcctcat taaaagcacc
     1741 ttttcctaat gaaagttgtg ctactggtat ctgtgtttga tttacaaaat cttctaattc
     1801 ttgatggagg tgaaaactgt taatttcatg tccagtaatg atgataggct gcttcgcttg
     1861 atgcagttta gttgctaata actctatata tgttgatgca tccgtatatt tagttgccgt
     1921 cacttcaaat ggtgtcggta tctcaatttc agagattgcg acatcgattg gtaaatgtaa
     1981 atgaactggg cgtctttcgg cgattgctgt attaattaaa cgtggtattt cggttgttgc
     2041 attttcaggt gtgatataac cttgtgcaac ggttatatgt gcaaacattt ttcgatagtc
     2101 gtcaaatgta ccttcaccaa gtgagtgatg tacatattta ccgccttgtt caacagcacg
     2161 tgtcggcgca cctgtaatcg caatgacagg tatgcgttca gcatatgaac ctgcgatacc
     2221 gttgacggca cttaattcgc caacaccaaa tgtagtaact aatgcagcga gtccattaag
     2281 acgggcataa ccgtccgctg cgtaacttgc gtttaattca tttgtatttc ctacccaatc
     2341 tacattggga ttgctgataa tatcgtctag aaaagcgaga ttaaaatcac caggaacacc
     2401 aaaaatttta tcgacgcctg ctcgatgaat agcgtcaatt aagtaagctc caatgcgttg
     2461 tttcataaaa aaagccacct tttctctata tttacactta aaatgatagc ctcgaatata
     2521 gtgtaaggtc aaggaagttg actttgttgt gaaaacgcta acaatagtga acaattaata
     2581 ttttttagga aatgagtatg aaggggggct ttaattgatg atcttaaaat tcaaaattta
     2641 aggggagagc gaactatcaa tgatgctgca aaatacaata gcaaagaaaa aacaggcgcg
     2701 tcaccttatg aaggtatacg cacctgttta tagtaagcat tatttagctt caaataattg
     2761 atcgccaaat gaaatgttgc catgttcacc ttgtttaaaa tcaaggtttg taatgtttcc
     2821 ttgtgtcacg ataataggcg taatatcact ctttgcatga ttgcggatgt agtctaaatc
     2881 aaagttgatt aataaatcac cttgtttaac ttcttgacct tcctcaacat gtaaagtaaa
     2941 gccttctccg tttaatttaa cagtgtctaa accgatgtgg attaatagtt ctaaaccact
     3001 atctgataca agaccaattg catgttttgt tgggaaaatc atttgtactt taccgttgaa
     3061 tggtgcacga acttcacctt gtgaaggttt gatagcgata ccgtcaccca tcattttttc
     3121 gctgaacact tgatcaggca cttctgataa tggtgttact tcaccagtta atggtgcatg
     3181 cacgatatgg ctcaattcgc ttgttgcaga tttatcttct gcaacaacaa cagtttcgtc
     3241 tttatcgtct tccatagtag taggattttc tactacttga ccattcataa tctgttgcat
     3301 ttcatgtttg atttggtcag atttaggacc aaaaattgct tgcatattat tgccgacttc
     3361 taatacacca gatgcgccta aatctttcaa accaggaaca tcaactttag atttgtcgtt
     3421 aacttcaaca cgtagacgtg tgatacaagc gtctaaatgt ttaatgtttg ctttgccacc
     3481 catagcttct aatactgcat atggtaattc agttgctgaa gcagtagccg cttgtgattg
     3541 tttatcttca cgacctggtg ttttgtattt taattttaca attaagaatc ggaatacgaa
     3601 gtagtaaata actgcgtata caagacctac aggaatgact aaccaccatt gtgtcttatt
     3661 aggtagtata ccgagtaaga agtagtcgat gaaaccacct gagaatgtat aacctagatg
     3721 aagatctaat aagtacaatg ttaagaatga taaaccatca agtactgcgt gaataaagaa
     3781 taataatggt gctacaaata agaatgagaa ttctaatggt tctgtaatac cagttaagaa
     3841 tgatgttaaa gcagcagaac ccattaaacc tgctactact ttcttatttt caggtttagc
     3901 tgtgtgataa attgctaaag ctgctgcagg taaaccgaac atcataacag ggaattcacc
     3961 ttgcatgaat ttaccagctg tcaaatgtgc gccttcacga atttgttcga taaagatacg
     4021 ttggtcaccg tgaataattt caccagctgc atttttccat gaaccaaact cgaaccagaa
     4081 cggtgcgtgg aaaatgtgat gtagaccgaa tggaattaat aaacgcttga tgaaaccaaa
     4141 taagaatacg gcaacaccag tatttgaatc taataatcct gtactgaatg catttaatcc
     4201 tgattgaatc gttggccaaa ttaatgccat tgggaatgct aaaataaatg atgttgtagc
     4261 catcataata ggtacgaaac gcttaccagc gaagaaacct aaataagatg gtaagttaat
     4321 gttatagaac ttgttataac accaagctgc cagggccccg attataatac cgccgaacac
     4381 acctgtttgt aatgttggga tacctaaaat gctagcgtaa ccactcgctg gatcaccaat
     4441 attcttaggt gtaacttgta aaaagtcgcc cattgttttg ttcatgatta tgtaaccgac
     4501 gaatgctgcg atagctgcta cgccatcacc gccagctaat ccgattgcga cacctaatgc
     4561 gaaaatcata ggcaagttat caaaaatgat accaccagca cctgtcatta atttagcgac
     4621 agtttgtacg ccaccatttt gtataaacgg caagtagtgt tgtaatgatt caccttgcat
     4681 agctgtaccg atagctaata acagaccagc tgctggtaaa atcgcaacag gtaacattag
     4741 cgctttacca atacgttgca attgaccgaa aagtttcttc ctcacttgtc caacctccaa
     4801 agttgtattt attatttaag attcaataaa aaaagacacg agcaaataga ctgtgttggt
     4861 aaccatcaca gcttaattta actcatgcct aatcttactt agtaacacgt tggtgtatgt
     4921 aattaatgta aaagaagcaa ttgactaagt agtatataaa actgatacgt tagtttatct
     4981 agcttaccat cacatcttat tgaatcttat ttgtttgcga ctcctatttt agcatggtga
     5041 agtatgcgtt ttcaatacaa ttattaaatt caatttcaaa tctcttaatt gttcacagat
     5101 tattattaaa acataatata agataatcct gaataaaatg tataaaagtt attgtaataa
     5161 tccagcagta gcagctatta tcagcactta aaagaaagga taatctttga tggtgtaata
     5221 attaacttgt gacagaaata tgtaagatta ttaca
//
LOCUS       DAKTLH010000159         5099 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.163, whole genome shotgun sequence.
ACCESSION   DAKTLH010000159 DAKTLH010000000
VERSION     DAKTLH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.163"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            240..743
                     /locus_tag="O0394_002397"
     CDS             240..743
                     /locus_tag="O0394_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0500456.1"
                     /translation="MKKRKKRKELDYKTRLAIAKYLNDGLNIIQISKILGRDDAVLYR
                     EVNKRSVDGVYNPLLAEEDAIKSRSISDEQVAKRRRLNDETKAYIEEKLSLKWSPRQI
                     ASQIEKDTGYYISYPTIYRYIRNGLVKVDVKRDMRQAGKKYNKSSEKRGKLDVGSRVI
                     KYRLKKY"
     gene            724..1260
                     /locus_tag="O0394_002398"
     CDS             724..1260
                     /locus_tag="O0394_002398"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCY0500457.1"
                     /translation="MGSKSINNREEFGHYEIDTVWSVRPSTYCLLTIIERKTRYLYAS
                     RLNTRKSNVVCNEIINIMKPLLPKSITMDRGKEFALFEEIEDELNCSIYFSDLGCPYQ
                     RGSIENANGLLRQYYPKGTDFANITQKSLDEAVKQINARPRMIFDYKSSEEMLKYHVS
                     TQNCEPILNDCVRHEPVN"
     gene            1480..2028
                     /locus_tag="O0394_002399"
                     /pseudo
     CDS             1480..2028
                     /locus_tag="O0394_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            2976..3350
                     /locus_tag="O0394_002400"
     CDS             2976..3350
                     /locus_tag="O0394_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCY0500458.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
     gene            complement(3601..4389)
                     /locus_tag="O0394_002401"
     CDS             complement(3601..4389)
                     /locus_tag="O0394_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0500459.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLKFLKSVS"
     gene            complement(4558..>5099)
                     /locus_tag="O0394_002402"
     CDS             complement(4558..>5099)
                     /locus_tag="O0394_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500460.1"
                     /translation="LMIQRNLFALQMNPVIFFIINFILISYLYDKQYVQFQAITGMSL
                     FYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIMGTIIAIISLLLIILQAFNITWGVIPI
                     TNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTVDAMIHNPQSAQIFLLIIWI
                     LLVVLVIYFTIRLSSRTRL"
BASE COUNT         1901 a          702 c          733 g         1763 t
ORIGIN      
        1 atacaaaagt ttcaagagta tataaaataa atcagactag cgataaatat gaagaagatt
       61 ctaatattga tgctgaaaag tttgagaaag ataataaacc agtttatgaa gaaaataata
      121 tgaaaaaata aagaatcaac tccctatacg ctttagcgat ggtatcggga gttgatgaac
      181 atatatcctc ttataactta ttttataaat aaccttttta aaagtaaagg aggatataca
      241 tgaaaaaaag aaaaaaacgt aaagaattag actataaaac acgtctagct atcgcaaagt
      301 atttaaatga tggtttaaac atcattcaga tttctaagat tttaggtcgt gatgatgcag
      361 ttctttatag agaagtaaat aagcgttctg ttgatggtgt ttataatcct ttattagctg
      421 aggaagatgc tataaaatcg cgtagtattt cagatgaaca agttgctaaa cgtagacgtt
      481 tgaatgatga aacaaaagca tatattgaag aaaaactttc attaaaatgg tctcctagac
      541 agatagcaag tcaaattgaa aaagatacag ggtattatat aagttatcct actatatata
      601 gatatattcg caatggattg gtaaaagttg atgttaagcg tgatatgaga caagcaggca
      661 agaaatataa taagtcttct gaaaagcgtg gcaaattaga cgtagggtct cgtgttataa
      721 agtatcggct caaaaagtat taataatcgt gaagaatttg gacattatga aattgatact
      781 gtttggagtg ttagaccatc aacatattgc ttgttaacta ttattgaaag aaagacaaga
      841 tatttatatg ctagtagatt aaatacacgt aaatccaatg tagtttgtaa tgagattata
      901 aatattatga aaccattatt acccaaaagt ataactatgg atagaggtaa ggagtttgct
      961 ctatttgaag aaatagaaga tgaattgaat tgtagtattt attttagtga tttgggttgt
     1021 ccatatcaac gtggttctat tgaaaatgca aatggacttt taagacagta ttatcctaaa
     1081 ggtacggatt ttgcaaatat aacacaaaag tcattagatg aagctgttaa acaaattaat
     1141 gcacggcctc gaatgatatt tgactataaa tcgagtgagg aaatgctaaa gtaccatgta
     1201 agcacacaaa attgtgaacc tatattaaat gactgtgtga ggcacgaacc cgtcaattaa
     1261 tataggttaa tctaagcgaa tggtttacaa tgagcgcgga aggacaggac gaataaattt
     1321 tgtatgtaaa atgcataatc taaaaagaag catactattt aaatctgtgg tttttgacag
     1381 atattactag taaaatgctt ctttttcttc ttctgtactc ttttagtaat atttattaaa
     1441 aaaatttaag aaattctcat ttttctattg acacactgcc atttaattaa agcaattgga
     1501 gtattgttac tcattgtcgg catcgtcata atgaatcaat ttaacaagaa taatttatta
     1561 ctaactgatc aaaagtattt actgttttgg cttctattag gatttatttt tggtttcttt
     1621 ccacctattc aaacgacaat taatagtgct ttagctagtc atactcattc accagccttt
     1681 gcatcattag tatcatttac aattgggtca atagcgctat tgattttaac cgctattttt
     1741 aatcgttctt taaaactaaa aacaagtcat ttaaaattcg gtaaattaaa gcctatctat
     1801 tttactggcg gtatacttgg catggctttt gtaacagcta acattatctt aatgcctcat
     1861 atgggtgcag cattaacaac acttattggg atgtttggcc agattctaat gggcatattg
     1921 atagatcact ttggattatt tggttcacct aaaatagcaa tgacatccag aaaaactatt
     1981 ggtctattat gtattttgac aggcattata cttttaagat tattttaaat taacttttag
     2041 cttatcattt taacttgtaa ttatttttaa aagtgataag ctattttttt gtggtctaaa
     2101 aatctttaga aaagcgttga tttgggtagt atgctttgac acaacaaatt ttaatttagc
     2161 aaattcgata gtcaactcat tcttaagacc taaattaatg ttatttttta ataatttaca
     2221 ccaaattaat agcaaaaatt atgttattcg tgctaatatt tcatagttgg ttattcaatt
     2281 aattaaaaat aagtcaaaat gcacaacttt ttataattca ttgagtcgag tttgaaaaat
     2341 aaaagtgctt taatgcatga tcaattatcg tactttctat tatttgttac ccgttatcaa
     2401 tcggaataac gtatagacac tttaacgtgc tatagattgg ttttaatcac taaattaatg
     2461 tgtttttctt atcattaaaa ctgcactgag aattactaaa ttaaaaaaat tataaaaatt
     2521 tttcattttt agtgataaaa ttctgaaaaa tgggtataaa tagtagaaga agttaacttg
     2581 gaagagttaa gctataacaa agaatctctt tagacacaca ttgaatatcg aaacatttaa
     2641 ttgcgctaaa tcgtttcatt aaataaatta ccttgtattg tcgattaaat taaggtaaat
     2701 tataaaaaat gctgatattt ttgactaaac caaatgctaa cccagaaata caatcactgt
     2761 gtctaatgaa taatttgttt tataaacact tttttgttta cttctcattt ttaattagtt
     2821 ataattaact aaataataga gcattaaata tatttaataa aacttattta atgcaaaatt
     2881 atgactaaca tatctataat aaataaagat tagatatcaa tatattatcg ggcaaatgta
     2941 tcgagcaaga tgcatcaaat agggaggttt taaacatggc aattacaaaa atcaatgatt
     3001 gctttgagtt gttatcaatg gtcacttatg ctgacaaatt aaaaagttta attaaaaagg
     3061 aattttcaat tagctttgaa gaattcgctg tattgacata catcagcgaa aacaaagaga
     3121 aagaatacta tcttaaagat attattaatc atttaaacta caaacaacca caagttgtta
     3181 aagcagttaa aattttatct caagaagatt acttcgataa aaaacgtaat gagcatgatg
     3241 aaagaactgt attaattctt gttaatgcac aacaacgtaa aaaaatcgaa tcattattga
     3301 gtcgagtaaa taaacgaatc actgaagcaa acaacgaaat tgaactataa ttttgtttag
     3361 cgcaatttgg tgaagtttga tagatgatac attctattaa acttcctttt tttatgctct
     3421 ttttacctaa ttgttaagag gttttgcact aatggcactt aatataagga agatagccgc
     3481 ataacgagca gtatatatca aatacatttc aaaaaaagct gatttctatc aaataatgaa
     3541 tagtaatcag ctttttcaca ttgcctaagg actttatgcc ctaggtttat atatcacata
     3601 ttatgatacg ctttttaaaa attttaaata ataattcata aatgcttctg gttcttcaat
     3661 atgcggtgca tgtcctgaat gttcgaagat ttttttcgta acatttaaaa aatgcgcttc
     3721 caatttaaaa tgattcttat ctttaatcaa aggatcatgt tccccattaa ttaacaaagt
     3781 aggtacacat gtacgttttt ttaaatacat aggttcatct ttatgcaaaa ttaatgaacg
     3841 aattgccacc gcactatcat cctcagactt aatgctatat attctttgtt tttgatacca
     3901 tttcatcgtc ttctcttgat ttctaaacaa tttagaaaat aaaagaatat acgcttcaga
     3961 cttattaaaa cccgctatat catcttgata ttgtattaac aattgagtaa agctgtgtat
     4021 taagtcatct ttcttcgatg caatggtagt taatgatttt acacgataag catatttatc
     4081 tgtaaataac ttagcaacta ccccacctaa atcatgtcct agaatatgtg cctcatgaat
     4141 atttaacttc tccattagaa tttttaaatc ctcaacgtga tcgtttaaat cgtatgattc
     4201 acttttagaa gacttgccat gacctcttaa atcgtaagta agtactttat atgatgatgc
     4261 tagttgatgt tgcaaatctt caaatccagc taaattacca tccaatccat gaagtaatat
     4321 tataggatcc cctttacctt caatttgata agttaattgg atttgaggat taattgtgac
     4381 tttattcatt gtgacacctc cctcaataaa ctatataccc aaccttcatt ctatatacgc
     4441 ttccatttta taaacaattt ttttaaatta tttataaatt accaaaagtt attccaacaa
     4501 taaaaaaact attcatctca attaatacaa ttaatcaagt gaatagtttt atcatattta
     4561 taatcttgta cgtgaagata atctaattgt gaaatatata actagaacta ctaataatat
     4621 ccatataatt aataaaaaaa tctgggctga ttgtggatta tgaatcattg catcgacggt
     4681 tttttgacaa aataataacc cgattcccga aaactcaagt cgcttataaa atatgccggc
     4741 aattactaaa ataataccaa tgaaaaagaa aaattgatgt ccaaaatttg taattggtat
     4801 aacgccccaa gtaatattaa atgcttgtaa tataattaat aacaaggata tgatagcgat
     4861 aattgtaccc ataatcattt ctattttatt actacgcaag taattctttt gagacaccct
     4921 taggtataca acaatagtca ttggaaaaat cactaaacaa taaaatagac tcattccagt
     4981 aatagcttga aactgaacat actgtttatc atataaatag gatatgagaa tgaagtttat
     5041 aataaagaaa ataactggat tcatttgtaa agcaaacaaa ttcctttgaa tcattaata
//
LOCUS       DAKTLH010000160         5007 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.164, whole genome shotgun sequence.
ACCESSION   DAKTLH010000160 DAKTLH010000000
VERSION     DAKTLH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.164"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            270..2084
                     /locus_tag="O0394_002403"
     CDS             270..2084
                     /locus_tag="O0394_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0500461.1"
                     /translation="MNKTKGFTKYKKMRYIPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WYKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
     gene            2287..4896
                     /gene="clpB"
                     /locus_tag="O0394_002404"
     CDS             2287..4896
                     /gene="clpB"
                     /locus_tag="O0394_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCY0500462.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
BASE COUNT         1862 a          638 c          930 g         1577 t
ORIGIN      
        1 tttttataat aaatttaatt tatattacag ttatattaca atacataata cttcatagtt
       61 tttaagttgc cgagttgcat tacaaattgg atttcgaagt aagaatcatc attggtaatt
      121 tttttaattt aacagtattt tagtcaatat gattttgatg atgtcgtaat tcaataatat
      181 acatatgcat ttcaatattc ttttaaacag cataactaaa tttaattaag catctaagaa
      241 gaacagtttt aaaagggaag aatgcaaaaa tgaacaaaac aaagggtttt acaaagtata
      301 agaaaatgag atatattcca gggctcgatg gtttgagggc aatcgctgtt ctaggaatta
      361 ttatttacca cttaaataag caatggttga caggtggctt tttaggtgtg gatacatttt
      421 ttgtgatctc tggttattta attacaagct tattactcaa agagtatgat gacacaggta
      481 tcattaaatt gaaaagcttt tggatacgtc gtttaaaacg tttattacca gcagtcatag
      541 ttttattaat ggttgtaggg acagcaacct tattattaaa atcagataat atcattaggg
      601 ttaaacatga tattattgct gcgatatttt atgtatcaaa ctggtggtat atagcaaaag
      661 atgttaatta ttttgagcaa ttttcattta tgccattaaa gcatttatgg tctttagcaa
      721 ttgaagaaca gttttacata tttttcccag ttattttggt tacattattg ttaacaatta
      781 aaaagcgata caaaatagga tttatttttt ggggagtatc aataatttct ttagggttaa
      841 tgatgtttat ctatagtatt aatggggatc attcacgagt gtattttggt acagatacta
      901 gattacagac attgttactg ggtgttattt tagctttttt atggccaccg tttaaattga
      961 aaaatgatcc acctaaagtt gtaaaatatg ttattgatag cataggtagt ttatcattta
     1021 tagtacttat attattattt ttcattatta atgatgagac gaattggata tatgatggtg
     1081 gtttctattt aatatccata ttaacgttat ttattattgc tagtgtcgtt catccatcta
     1141 catggatagc gaagatattt tcaaatccag tgttagtatt tatcgggaaa aggtcttata
     1201 gtttatattt atggcatttt gcagtaatta gtttcgtaca tagttactat gtagacggac
     1261 agatacctgt atatgtgtac tttatagata taagtttaac aattatattt gcagagctat
     1321 catatcgctt tatagaaact ccatttagaa aagaaggtat taaagcttta aattggcgac
     1381 cttcttatat accacaattt ataagaatgg caattgtagt aaccttgtta attccattta
     1441 tgttgatttt agtaggtgca ttcaataaat atggtaaaga cattattgga gaaaaagcga
     1501 atagctttga taccacaatt gaagataatt atttaatgcg gatagcacca attgataaca
     1561 tacacattga tggcttagta agtgagaaga aaaaggaatc ttccgacgta tataataata
     1621 ttaaacctct tttaatcggt gattcagtaa tggttgatat cggtgagtca tttaagtcat
     1681 cagttcctaa gtctagaatt gatggaaaag tagggcgtca attgtatcaa accttacctt
     1741 tagttaaagc gaattattca caatataaaa aatcatctga tcaagtcgta ttagaattag
     1801 gtacaaatgg cgactttact gtcaaacagc tcgacgattt acttaatcaa tttggaaaag
     1861 ccaagattta tttagttaat acacgtgttc caagaattta tgaggcaaat gtaaatcgat
     1921 tattagctga cgcggcgaaa cgaaagtcca atgtcacatt aattgattgg tataagcgat
     1981 cacaaggaca tagtgaatat tttgcaccag acggtgtaca tttagagtac aaaggagtcc
     2041 tagctttaaa agatgaaata ttaaaagcac ttaaaaagaa ataacatatg tttaataagt
     2101 ctagttaatg tgtaacgtaa cattagctag atttttttat tcaaaaaaat atttacaaat
     2161 attaggaaat ttaagtgtaa aagagttgat aaatgattat attgggacta taatataatt
     2221 aaggtcaaag aaagtcaaag tcaaaagtgc aacttgacca actctacaaa ttagaggtga
     2281 aattatatgg atataaataa aatgacatat gctgttcaaa gtgctttaca acaagcagtt
     2341 gaactgagtc agcaacataa attacaaaat atagaaattg aggcaatttt aagcgctgcc
     2401 ttaaatgaaa gtgaaagctt atataaaagt attttagaac gagcaaatat tgaggtagat
     2461 caattaaaca aagcttatga agacaaacta aacacgtatg catctgtaga aggtgacaat
     2521 atacaatatg gtcaatatat tagccaacaa gcaaaccaat tgataactaa ggctgaatca
     2581 tacatgaaag aatatgaaga tgaatatatt tcaatggagc atattttacg ttcggcaatg
     2641 gacattgatc aaacaacaaa acattatata aataataaag tagaagttat caaagaaatt
     2701 attaaaaaag taagaggggg aaatcacgtg acatcacaaa atccagaagt taattacgaa
     2761 gcattagcta aatatggccg cgacttagta gaagaagtta gacaaggtaa aatggatcct
     2821 gttataggaa gagatgaaga aattcgaaat acgattcgta ttttaagtcg taaaactaaa
     2881 aacaaccctg tgctcattgg tgaaccaggt gttggtaaaa ctgcaattgt tgaaggatta
     2941 gcgcaacgta tagttaagaa agatgtgcca gaatcattat tagataaaac tgtttttgag
     3001 ttagatttaa gcgcattagt agcgggcgct aaatatcgtg gtgaatttga agagagatta
     3061 aaagcagtcc taaaagaagt taaagagtct gatggtagaa ttatattatt tattgatgaa
     3121 atccatatgc ttgtaggtgc tggtaaaaca gatggtgcca tggatgcagg caacatgcta
     3181 aaaccaatgt tagcacgagg agagttacat tgtattggtg caacaacttt aaatgaatat
     3241 cgagaatata ttgaaaaaga ttcggcatta gagcgtcgtt tccaaaaagt agcagttagt
     3301 gagcctgatg ttgaagatac aatttcaatt ttacgtggtt taaaagaacg atatgaagtg
     3361 tatcatggtg tgcgtattca agatagagcc ttagttgctg ccgctgaatt gtctgatcgt
     3421 tacatcactg atcgtttttt accagataaa gcgattgatt tagttgacca agcatgtgca
     3481 acaattcgta cggaaatggg atcaaatcca actgaattgg atcaagttaa tagacgtgtc
     3541 atgcaattag aaattgaaga aagcgcactt aaaaatgaat ctgacaatgc gagcaaacag
     3601 agattacaag aactacaaga agagcttgcc aatgaaaaag agaaacaagc agcacttcaa
     3661 tctcgtgtag aatcagaaaa agaaaaaata gcaaatttac aagaaaaacg tgcgcaacta
     3721 gatgaaagta gacaagcgtt ggaagatgca caaacaaata acaatttaga aaaagctgct
     3781 gaactacaat atggaacaat tcctcaattg gaaaaagaac ttagagaatt agaggataat
     3841 ttccaagatg agcaaggtga agatacagat cgaatgattc gtgaagttgt aacagacgaa
     3901 gaaattggcg atattgtcag ccaatggaca ggcataccag tttcaaaatt agttgaaaca
     3961 gaacgtgaaa aattacttca cttaagtgac atcttgcata aacgtgttgt aggtcaagat
     4021 aaagcggttg acctggtttc agatgcagta gttagagcaa gagcaggtat taaagatcca
     4081 aacagaccta ttggtagttt cttattccta ggtccaactg gagtaggtaa aactgaatta
     4141 gctaaatcat tagctgcatc attatttgat tctgaaaaac atatgattcg tattgatatg
     4201 agtgaatata tggaaaaaca tgcagtatca agattgatag gggcacctcc aggatatatt
     4261 ggacatgatg aagggggtca attaactgaa gcggttcgtc gtaatccata ctcagttatt
     4321 ttattagatg aggttgaaaa agcgcatact gacgtcttta atgtattatt gcaaatttta
     4381 gatgaaggcc gtttaactga ttctaaagga cgtagcgttg attttaaaaa tactattatt
     4441 attatgacaa gtaatattgg atctcaagtt ttattagaaa acgtaaaaga gactggtgaa
     4501 attacagaat caacagaaaa agctgttatg acaagtttaa atgcatattt caaaccagaa
     4561 attttgaatc gtatggatga tatcgtatta tttaaaccat tatctattga tgacatgagt
     4621 atgattgtag ataaaatctt aacgcaatta aatataagat tattagaaca acgaatctca
     4681 attgaagttt ctgatgatgc taaagcttgg ctaggtcaag aagcttatga acctcaatac
     4741 ggtgcaagac cattaaaacg ttttgtacaa cgccaaattg aaacaccatt agcacgtatg
     4801 atgattaaag agggattccc agaaggtaca acgattaaag ttaatttaaa ttcagacaat
     4861 aacttaacgt ttaatgttga aaaaattcat gaataaaatt gaaccaagaa tgtgatgatt
     4921 aatcgcattc ttggtttttt agatttgaaa tttattataa ttgttcattg gcaatatact
     4981 ttttaaatga tttaataaac accaata
//
LOCUS       DAKTLH010000161         4959 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.165, whole genome shotgun sequence.
ACCESSION   DAKTLH010000161 DAKTLH010000000
VERSION     DAKTLH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.165"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            94..297
                     /locus_tag="O0394_002405"
     CDS             94..297
                     /locus_tag="O0394_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCY0500463.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            complement(294..530)
                     /locus_tag="O0394_002406"
     CDS             complement(294..530)
                     /locus_tag="O0394_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500464.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            755..1336
                     /locus_tag="O0394_002407"
     CDS             755..1336
                     /locus_tag="O0394_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCY0500465.1"
                     /translation="MAKTLYIMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGIFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEERIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            complement(1409..2026)
                     /locus_tag="O0394_002408"
     CDS             complement(1409..2026)
                     /locus_tag="O0394_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HCY0500466.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGETQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF"
     gene            complement(2181..2675)
                     /locus_tag="O0394_002409"
     CDS             complement(2181..2675)
                     /locus_tag="O0394_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0500467.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
     gene            complement(3074..3496)
                     /locus_tag="O0394_002410"
     CDS             complement(3074..3496)
                     /locus_tag="O0394_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCY0500468.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            3644..4360
                     /locus_tag="O0394_002411"
     CDS             3644..4360
                     /locus_tag="O0394_002411"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCY0500469.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFTNDSYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     VPQAPGQIDVTDLKAQVTLY"
     gene            4443..>4959
                     /locus_tag="O0394_002412"
     CDS             4443..>4959
                     /locus_tag="O0394_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HCY0500470.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEGDMPKAPRKN"
BASE COUNT         1763 a          733 c          806 g         1657 t
ORIGIN      
        1 aaatcacttt tgcacattta caaatactag ttttctgata agattaataa taacacttta
       61 aatagcgctt aataaatgaa gggggcaagt cttatgacgt tttacaattt catcatgggt
      121 tttcaaaatg ataacacacc atttggtata ttggccgaac acgttagtga agataaagca
      181 ttccctcgat tagaagaaag acaccaagta attagagcat atgtgatgtc taattacaca
      241 gatcatcaat taattgaaac tacaaataga gctattagct tatatatggc aaattaattt
      301 gagtagtacc aattatgatg tattagtgca tcccaaatat cttttgtttt aaagtttatt
      361 tcatcatttc ttatcgaaaa tggtgtaata atgtctttat ctaaccaagt gttgataagt
      421 tcatttggta caccatctaa caacatttca cttttactaa ttataaaaca ttcccagtca
      481 agtgaaacat tttgtggatt cacataatta cattgattat gattatccat aaacactcac
      541 tcctttaaaa ttctgtactc ttcattgcgt tttaccccgt cacattatct tttaaactaa
      601 aatcatcatt acttatgaaa aaaatgtaca tcaaaagcaa aggttttcgc taccgaaaaa
      661 gtttaaataa tggtttaata tatttggtac tcattttaat aaaaagagaa tacattttga
      721 gctatcaata ctttttattg aagaggtgtt attgttggct aaaacgttat atataatgcg
      781 ccacggacaa actttgttta attttaaggg actaattcag ggatttggag attcgccgct
      841 aacagaactt ggaattgctc aagctcaaaa ggcacgtagt tattatgaaa ctaaggggat
      901 aaacttcgat ttatatgcat catcaacgca agaacgcgca agtgacacac ttgaaaatgt
      961 tgcacctaac caatcgtatc aacgttttaa gggactgaag gaatggcatt ttggaatatt
     1021 tgaaggtgag tcagtctatc tatttgataa tctatacaag cctgaggact tattcggaga
     1081 tcgaattgtt cctttcaaag gagaggcaag gcaacaagtt gaagaacgca ttgtgaaaac
     1141 tttacatgac attatgtctc aaacaaagaa taatgcatta gtcgtgagtc atggaacaat
     1201 aatgggagta tttttaagat attgccttaa actagatgaa gcattaaagc ataatatcgg
     1261 taattgtaat atcctgaaat ttgaatatga caatggaaca tttaaatttg ttgagttaat
     1321 tgatccaaat ttataataaa taaagcgtta tacatagaca caagtatgtc ttttgtataa
     1381 cgcttttaaa atacaactac ataacgcttt agaataataa ttgaataagt ttttgtaata
     1441 tcatcagagc aacaataata attattatgc ttgatatttt attgattatt gttaataatt
     1501 ttccggtttt atcaattgat ccaaccattt ttcctaaaat tgcgagtaaa aagaaccata
     1561 accatgaaac gctaatacaa gcgattgtaa acgcaatttt attgctgcca ctatataatg
     1621 cagcactact accaattact ccaattgtat ctaaaatagc atgtggattg agtaatgaaa
     1681 ctgataaagc aaaacttact tgtttcattg gagacataat ttgagtttct ccatctgttg
     1741 agggtttatc atgccaaatg gtccaagcca tatacatcaa gaaaattaaa ccaactatat
     1801 aaataattgc ttgaagtaca ggtaaagaca taataatgat agatactcct accactgcaa
     1861 taataataag taagctgtct gacaacccgg ctgtaattat tgcaggcaat acatatctat
     1921 attttggttg attagctcct tggttaaaaa taaatacatt ttgtgcacct aaaggtaaaa
     1981 ttagaccaat cgctaagata aatccatgaa taattgcggt taccattaag ttttgacacc
     2041 cctaaaacat agaatattct gaaaaaactt attgttatca tatcaaataa aaaacaacta
     2101 ctcaatagta gagtagttgt atgaaaaata gaacctatat gatttgaatt tatgagaaat
     2161 gtagaatata taaaactgta ttacttatta atcaaatcat aaaaaagcca atgttcatcg
     2221 aaataggtat ttccgatttt aattgcattt ttctcaaaac caagaatgtc gaatcctagt
     2281 gcactataga aaactttagc acctatgttg tttgacacaa ttgatgtaag taaagattcg
     2341 taattgttct gccgtgcata attaataatg aaattaatga gctcgctatt aatcgattta
     2401 tcgttattag taacaaagtt gtatttaatt aatgatttat gttcttttcc aacgtatcga
     2461 atttgttcta aggcagctgt tgcgataagt gtttcgtttt cataacagcc aaatacaata
     2521 catttcggtg aaccttgatt taaaatgtca tgtattaagg tgtcagtcat tgtttgacta
     2581 taatgtgaat cttgagtgaa ttcttcattg actgtcgaaa caagtctctt atactcatta
     2641 aaatcattat tcgttaatgt tttaatttca atcattactg acctccttaa tcattattaa
     2701 gaaagatttt gtactaaaaa aatttcttaa ttacatatta aaatgtttat taaaattttg
     2761 caatcactga tttatatgca cattcttgta aatggattat caatttaatt atatttattt
     2821 ttaagttcag gaaattatgg aattggttat tggtcgacac tggactttta tttgtagacg
     2881 gtatgcgaag aagtcaagcc agtattggaa ccggtcacat ttatgatgac taaggtacaa
     2941 tcattttcag ttaaatattg ccagaaattt caatcacgaa tagaaaaaat aaccaaccaa
     3001 aaatttttgg ttggcaaatt tgaattttat gtgaatttta ttaataacgt tgaataatct
     3061 ctttaagtta atgctaatct acaacattta catttaaatc gacattaata tttccttgag
     3121 tcgcttttga atatggacaa aattcatgag ccatttgtaa atatttttca gcttcttctt
     3181 gagatataac atttttaatt gtcgcatcaa ttgaaacact taatttagga ctttctgagt
     3241 ctgaatcatc ttctagtctc actgttagtg ttacttctgg atgagcatca cgcactttgt
     3301 tttgctttaa aattaggtcg aaagcaccgt tgaagcaaga tgcataacct gctgcaaata
     3361 attgttcggg gttagtagct ttaccatctg cttgagcagg cggaacgata tcaatatcta
     3421 atgctcgatc atcagtataa acatgtcctt tacgtccgcc aacattcgtc gctttagttt
     3481 cataatgtat tgccatactt ataacctcct aatttaaaat acatttatgc tttacccatt
     3541 cgaaaatacc ttaatcattt tcatttatag cattgtttga ttgaaggata aaaagttgtt
     3601 gtttacaata aaaataatga gtatctgaaa tgagggattc actatgacac atgtggaagt
     3661 agtagcgact atcgcgccac aattatctat cgaagaaact ttaattcaaa aaattaatca
     3721 tcgtattgat gcaatagacg tattagaatt acgaattgat caaattgaaa atgtcacagt
     3781 tgatcaagtg gcagaaatga ttacaaagct gaaggttatg caagattcat tcaaattatt
     3841 agttacgtat cgtacaaagt tacaaggtgg ctatgggcaa tttacaaatg actcgtatct
     3901 taatttaata tcagacttag caaatatcaa tggcatagat atgattgata tagaatggca
     3961 agcagatatt gacattgaaa aacatcaacg aatcattaca catttgcaac agtataataa
     4021 agaggtggtt atatcacatc ataatttcga aagtacgcct ccattagatg aattgcaatt
     4081 tatatttttt aaaatgcaaa aattcaaccc agaatacgtt aaattagcag taatgccaca
     4141 taataaaaat gatgtgttaa atttattgca ggcaatgtct acattttcag atactatgga
     4201 ctgcaaagtt gttggtattt caatgtctaa acttggacta ataagtagaa cggctcaagg
     4261 cgtttttggt ggtgcgttga cttatggttg tatcggagta ccacaagctc caggacagat
     4321 tgatgttact gatttaaaag cacaagtgac tttatactaa taaaatgaat taggttgctt
     4381 ttgtgtaagt gctgtcttat aattgagaag tattatcatt gttcgtggag gagcgatttt
     4441 agatggaatt acaacaagca atagctaata gaagaagtgt gaaaaaattt aaaagagata
     4501 tgcacataga tgacgcattg ctatatcaag caattgagaa agctgctgat gctccaaatc
     4561 acggaatgag ggaaccatgg agagttgtgc atgttccgaa agacagatta ggagatatga
     4621 gtaaggatat ttctaaattt gcatttccta atgaattaga taagcaacaa tgtcattatg
     4681 atgcagttac gaaactaggt ggcatgttat tgcttatttt aaaaacagat ccaagacaac
     4741 gtcaaaatga tgaaaactac tttgcatttg gtgcatatgc acaaaatctt atgttgttac
     4801 tttatgaagc gggaataggt acatgttgga aatcgccatt atatatctat gatcctaaag
     4861 taagaaaaac acttggtata aagaaagatg aagttcttgc tggattctta tatttaacgg
     4921 atttagaagg agatatgcct aaagcaccac gtaaaaata
//
LOCUS       DAKTLH010000162         4953 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.166, whole genome shotgun sequence.
ACCESSION   DAKTLH010000162 DAKTLH010000000
VERSION     DAKTLH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.166"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(359..1105)
                     /locus_tag="O0394_002413"
     CDS             complement(359..1105)
                     /locus_tag="O0394_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HCY0500471.1"
                     /translation="MPIEYFTHQFEEITYLSDDLQVKALMMTPHHEVNRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     HAFIHMVGFSRGGLQGLLTFQDLPVTSYTIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVESHS"
     gene            complement(1110..1583)
                     /gene="ytkD"
                     /locus_tag="O0394_002414"
     CDS             complement(1110..1583)
                     /gene="ytkD"
                     /locus_tag="O0394_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HCY0500472.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIYYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            1649..1906
                     /gene="yidD"
                     /locus_tag="O0394_002415"
     CDS             1649..1906
                     /gene="yidD"
                     /locus_tag="O0394_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCY0500473.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(1903..2904)
                     /gene="menC"
                     /locus_tag="O0394_002416"
     CDS             complement(1903..2904)
                     /gene="menC"
                     /locus_tag="O0394_002416"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCY0500474.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVADGTIPPIAL
                     DEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGAKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(2909..4387)
                     /gene="menE"
                     /locus_tag="O0394_002417"
     CDS             complement(2909..4387)
                     /gene="menE"
                     /locus_tag="O0394_002417"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCY0500475.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMKSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGRDITTNSLLDNTMGIQYETSNETVVPKESPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQK
                     ILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNVYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISK
                     AQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(4546..>4953)
                     /locus_tag="O0394_002418"
     CDS             complement(4546..>4953)
                     /locus_tag="O0394_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HCY0500476.1"
                     /translation="STNNKIVGQRFEIQQHNETIGSIYFSADYAHIRGIEKGTAKYFI
                     DKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYVYLIENLDDEVFHLSALQD
                     MNRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
BASE COUNT         1457 a         1006 c          771 g         1719 t
ORIGIN      
        1 gtgttgatta ttttggttgg ctgaccaata tttattctag cacgtagaga tgcatttttt
       61 gtgacaatgg tagaaccttt tctgaaccat acgcatagcg tatggttttc tttttacaat
      121 taaagagcca accgttgtta tagtctaaca atggttggct cctcttattt tatgtgctaa
      181 aaatttatag gcaattttat tacaacaatg tacatttaag gtgaccttca tgccaaaatc
      241 gcatcactca tttaatggaa gcagcacgtc ttcatataaa gtaccgatcc ctaattcaac
      301 gcatgtagta ccacatcttc aaagcttgat agttcccatg cgcacaccac gtttcatact
      361 agctatgcga ctcaacttgg ttcataaact ctttaatata agtcaatgtt tcaaccatcg
      421 ctggtggtct tggcacatgt ccttctgcca tttgataaaa tgtttcatgc gtggcacctt
      481 ttaactctag ttggtccgct aaataatacg catgatgaat accaacttgc tggtctttcc
      541 ctccatgtac aattaatatt ggcggactgt tttcattaat gtttggaatc gcttggcgtg
      601 cctcatatgc cgctcgatct tttttcggat gaccaatcat tcttcgtagc atgcctctta
      661 aatcgacacg ttcttcatac attaaatcaa tatctgagac accaccccag attgtataac
      721 ttgttactgg taagtcttga aatgtcaaca atccttgtaa accacctcgc gaaaaaccaa
      781 ccatgtggat aaatgcatgt ggatatttat catgtagcaa ccttaataat tgcgtcacat
      841 catttaaatc gccacggtaa aattcgtctt tgccttcact cccattgtta cctcggtagt
      901 atggcccaat cactaaagtt tgactatctg aaaattgcat taatctacct gcgcgcacac
      961 gtcctacttg acctttgcca cctcgcaaat aaactacaat gcgatttact tcatgatgtg
     1021 gtgtcatcat taaagctttt acttgtaagt catctgacaa atatgtaatt tcttcgaatt
     1081 gatgcgtaaa atattcaatt ggcattcgtt tacgtttgat aaaacccaag tgattgcacc
     1141 ctctctacgc attttaaaat ggtactatct tgcagtaaga aactccgttg tgcgagttca
     1201 atatcattga tacagttaaa caacactggc cctgctgttt ctaaataatc gttcttgctt
     1261 accaatgatt caacttcgat aaaatataca tcttttacaa aatcagtttg atcatgtgtt
     1321 tcaatggtat attgtgctat gtaataaata tttttaactt tggcgcctgt ttcttcatat
     1381 aattcacgtg taactgcttc agcactactt tccccgcgtt cccttttacc accaggaaat
     1441 tcaatccccc gtaaattatg tttggtaaaa agcaattgat ttttaaacgt tggaatagct
     1501 agcacatgat tgccatctgc tatctcatta tcctttttaa atgtcaaatt aacttgacga
     1561 ttatctttat ccctaaactt cacgcgcatc acatccctac attgtatgtt aatataatag
     1621 ttaattacta tcgttggagg cattaattat gaaaaagata ttcttggcga tgattcattt
     1681 ttatcaacgt ttcatttcgc cactcactcc accaacttgt cgtttttatc caacatgttc
     1741 agagtacact agagaagcga ttcaatacca cggtgctttc aaaggccttt atttaggtat
     1801 ccgtcgtatt ttaaaatgtc atccgcttca taaaggcggc tttgaccctg ttccgttaaa
     1861 aaaagacaag tcagcaagca agcattcaca taaacataac cattaatatg gttgtaattg
     1921 agttatatcc actaaagggg ggcgaaattc gagtcgcccc tcttttaata tgcctgaatg
     1981 cgccaccaca tcttgttcaa aataataacc tgctggtgta acatctcctg gataatcacc
     2041 tttacgagca agcatcgctg taaaatagcg gcttaaacca tattcgtaca tgccgccaat
     2101 aaccactttt gcaccatgac ttttcaaagt atcaattgcc gtttgcactt tatcaatgcc
     2161 acctagacga aatggtttta atacaacaac tttcacattg tataattcta tcaaattaat
     2221 tatgtccaac aacgatgttg ccttttcatc aagggctatt ggaggtattg ttccatccgc
     2281 tacttcatca agcatggaga tatctttaaa tggctcttcg atataaagaa cctgttcacg
     2341 cgctaataac tgtaactgtg tgaaatcttg acgatccaag gactcatttg catctataac
     2401 caattgaaag tgaaagtcta attcccgtaa cactctaatt tgatgcatga tttgaggcgt
     2461 ccattttaat ttaattctgg tcggctttgt tgcttttaat gactctagtt gtttatttga
     2521 taagccgctc gctgtcgctc catatgctac tgaaaatgaa ggcagtacat gaaacatttg
     2581 atacaatgcc atgacaatag ttgcccttgc agcaggcgta ttttccaatg aatctactaa
     2641 ttttagtgct gcttcatacg tttcaaatga tttatttcta ttatcttcga accattgctc
     2701 aattacatgt ttcactgagg caattgtttc atgatcatac caatctgttt gaaaagcgtt
     2761 acattccccg aaatatgcat ttcctttgtc atcaatcaat tcgataaaca aacaatcacg
     2821 atgcgttaaa gtgactttcg gtgttacaat ttgtgactta aatggctcac tatatttata
     2881 aaaatgcaaa gctgtcaact tcatcaaatc atcctctata caacttattt ctttgtaatt
     2941 tacctgttga tgtataaggt aaagtatcaa ccttttcaaa gtgtttcggt actttatatt
     3001 tcgctaaatg ttgtgataaa tatgcaatca attgtgcctt tgaaatgtca ctttcactga
     3061 caaaatataa tttaggcact tggccccaag tatcatcagg atgccctaca catactgcgt
     3121 cactgatacc tggaaattgc ttcgctaccg tttcaatttg atatggataa atattttcac
     3181 cgccactaat aattaaatct ttacgtcggt cataaatcat gacataacct tcatgatcta
     3241 tttcagcaat gtcacccgta ttaaaataac cattttcaaa cgtacccgtt aaatctgttg
     3301 gatacaaata tacattcatc acattggcgc ctttaatcat taattctcca tgaccttctt
     3361 tattaggatt tttaattttt acgtcaacat tggcacttgg catccctaca gtgtcaggac
     3421 gtgcatgcaa catttccggt gttgctgtta aaaattgcga acatgtctca gtcataccaa
     3481 atgaattata aattggcagg ttatattgta atgccgtctc tatcatagtg gcagataatt
     3541 tagcaccgcc gagtaatatt ttttgtaaat tataaggttc atgtaaacct tgttgcataa
     3601 gccaatttaa agtttgtggc acaagcgaaa tgtgcgtgat tcgttcattt ttaatcatcg
     3661 ttaaaatttg ttcggcattg aatttatcaa caatgcgcac agtaaaacct tcaataacag
     3721 ctcttaaaag tacactgaga cccgaaatat gataaatcgg caagacagat agccaattag
     3781 tgtcacgatc aaatcccaag ctctctttac atccgattgc actggcataa tgattacgaa
     3841 acgtttgtgg caccgctttt tgagggcccg ttgtccctga tgtaaacata atcgatgcaa
     3901 tgtcatctaa attaaatgat gtatttaata tgttggacgg cgactctttc ggcaccacag
     3961 tttcattcga tgtttcatat tggataccca ttgtgttgtc caacaaactg ttcgttgtaa
     4021 tatcccttcc agcgaattca atatcatcca gcgatacaat ttgaaaccct cgtaattcca
     4081 gtggcaaggt acaaaaaatc aattgtacat cgattgactt catctgattc gtcatctcat
     4141 taggtgtcaa ccttgtatta atcatcgcaa tttcaatatt tgccaaccaa catgcatgta
     4201 ttaaaatgat cgattgaatc gaattatcta tgtatagccc aacacgagat tgttgataag
     4261 ccttgagtct tttagccaat agactcgctt cacagtataa attttgataa gtataagatt
     4321 cttgaccgtc tgttatcgca atatgatgtc cattttgttg tgcttgttta tataaccaaa
     4381 agtccatgcg ttattcctcc aaaatcattt acattataat tataacgatt ttatgacatt
     4441 ctagcagtgg ttatgtttaa aaatataaaa aagtagacga attgatgcat tgatatgatt
     4501 gttataatgc tcaatacata tcgttatatc attcgtctac tattatcagt tatttttatt
     4561 taattttagt gtcattctgt cattttgatg tggtgattta cccattgttg ccacatcatc
     4621 tgcaatgtca attggtatac ggttcatgtc ttgtaatgca cttaaatgga atacttcatc
     4681 atctaaattt tcaatgagat atacataata tgttaccttg tcctttttat attttaacgt
     4741 tttccaaaag tccggcttgc aattcaatac attatccgga atatattcaa taaataagta
     4801 acgtttgctg cctactttgt ctatgaaata ttttgcagtg cctttttcta tacctcttat
     4861 atgtgcatag tctgctgaaa agtaaatact acctattgtt tcattatgtt gttgtatttc
     4921 aaatcgttgg cctactattt tattatttgt gct
//
LOCUS       DAKTLH010000163         4910 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.167, whole genome shotgun sequence.
ACCESSION   DAKTLH010000163 DAKTLH010000000
VERSION     DAKTLH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.167"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..379
                     /locus_tag="O0394_002419"
     CDS             <1..379
                     /locus_tag="O0394_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin MazF"
                     /protein_id="HCY0500477.1"
                     /translation="LFLNLISAQNNSAILKEVFDKKYQKNNTEFITKDYFSEFISDSL
                     EIENKLNKIDRNINNIVSAIDKVKKLKGNSYACINSFQPISKFRIRKVLPQKIKNPVI
                     DSSDIMLLINRINNNILQIPDIR"
     gene            570..1607
                     /locus_tag="O0394_002420"
     CDS             570..1607
                     /locus_tag="O0394_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCY0500478.1"
                     /translation="MKTRCYDGKKWQYEFKYEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFI
                     DERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLHAINNALKSAC
                     RVNNISIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DKKTTKILESM"
     gene            1658..2488
                     /gene="sph"
                     /locus_tag="O0394_002421"
                     /pseudo
     CDS             1658..2488
                     /gene="sph"
                     /locus_tag="O0394_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(2726..3742)
                     /gene="lukG"
                     /locus_tag="O0394_002422"
     CDS             complement(2726..3742)
                     /gene="lukG"
                     /locus_tag="O0394_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HCY0500479.1"
                     /translation="MIKQLCKNITICTLALSTTFTVLPATSFAKINSEIKQVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKDKMPVTVSEGFNPEFLAVMSHDKKDKGKSQF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHNVKFVKVLND
                     NEKK"
     gene            complement(3764..4819)
                     /gene="lukH"
                     /locus_tag="O0394_002423"
     CDS             complement(3764..4819)
                     /gene="lukH"
                     /locus_tag="O0394_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HCY0500480.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKDKRNVTNKDKNSTAPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKT
                     VKVVDKYSDDNKPYKEG"
BASE COUNT         1701 a          768 c          719 g         1722 t
ORIGIN      
        1 attgttttta aatttaatta gcgcgcaaaa taattcagct atattaaaag aagttttcga
       61 taaaaaatac caaaaaaaca acacagaatt catcactaaa gattatttta gtgaatttat
      121 atctgatagt ttagaaattg aaaataaatt aaataaaatt gacagaaaca ttaataacat
      181 agtatcagca attgataagg taaaaaaatt aaaaggtaat agttacgctt gcataaattc
      241 tttccagccg attagtaagt ttcgcataag aaaagtttta ccccaaaaaa ttaaaaatcc
      301 agtaatagat tcttcggata ttatgttact gataaataga attaataata atatattgca
      361 gattcctgat ataagatgat ataattttaa tatattaaag gtttatcctt taaaacacgt
      421 atatattcgt taccattttt ggtaattaac catgtaatct tataactata agtggcgtct
      481 gtattttata caggcgtctt tttttataca attttcatgg gtagcccgcc tacccttatt
      541 attttttgcc aattttgagg agggaacgca tgaaaacacg ttgttacgat ggtaaaaaat
      601 ggcaatatga atttaagtat gaaggaaaaa gataccgtaa gaaaggtttt agaacaaagc
      661 gtgaagctaa ttctgctgga ctagacaagt taaatgagtt aagaagtggt tttaatatag
      721 ataactatat aactcttgaa gaatacttcg aaaattggat taaaacgtat aaacaacctg
      781 ttgttaaaga aaatacctac cgtcattata gaaatgcatt acaacatata caaaaacata
      841 aaataggtaa aatggagtta tcaaagataa atagacaagt ttatcagaaa ttcataaacg
      901 attattcaaa agaacacgca aaagaaacta taagaaaaac aaacggtgct attcggtcag
      961 ctttagatga cgcattatat gatgggctta tttttaaaaa tcccgcttat aaagttaatt
     1021 ataaagccgg aaaacctacg aagtcagaac aagaaaaatt catctcggta actgaatatg
     1081 aaatactaaa agatcacgtc agaaagaaga gaactcgttc atcattagcg ctattcataa
     1141 tgatttgtac gggttgtcgt gtcagtggtg caagaaatat aaagattgag catatcaacc
     1201 aagtgaaaaa cactatattt attgacgagc gaaaaaccga tacttcccct agatatatca
     1261 gtatcgctaa atctgatatg aaacacatta tggacgtcat aagtacattt gcaattagct
     1321 atgatggtta cattttcaaa gaagccggat ctataattaa ccttcatgct atcaataatg
     1381 ctttgaaatc agcctgtaga gtcaataata tatcaattat tacatcgcac gcattaagac
     1441 acactcattg ttcttattta ctagcaaaag gtgtatctat acattacatt tctaaaagat
     1501 taggtcataa aaatatagca ataactacat ccgtgtattc tcatttgtta gaagaaaaat
     1561 ttaatgaaga ggacaaaaaa acaactaaaa ttttagaaag tatgtaattt agggacccat
     1621 tagggactcc aaacccaata aatactgttg ttacaaggtt tctatgtatc caaactgggg
     1681 gcaatataaa cgcgctgatt taatcggaca atcttcttat attaaaaata atgatgtcgt
     1741 aatattcaat gaagcatttg ataatggtgc atcagacaaa ttattaagta atgtgaaaaa
     1801 agaatatcct tatcaaacac ctgtactcgg ccgttctcaa tcagggtggg acaaaactga
     1861 aggtagctac tcatcaactg ttgcagaaga tggtggcgta gcgattgtaa gtaaatatcc
     1921 tattaaagaa aaaatccagc atgttttcaa aagcggttgt ggattcgata atgatagcaa
     1981 caaaggcttt gtttatacaa aaatagagaa aaatggtaag aacgttcacg ttatcggtac
     2041 acatacacaa tctgaagatt cacgttgtgg tgctggacat gatcgaaaaa ttagagctga
     2101 acaaatgaaa gaaatcagtg actttgttaa aaagaaaaat atccctaaag atgaaacggt
     2161 atatataggt ggcgacctta atgtcaataa aggcactcca gagttcaaag atatgcttaa
     2221 aaacttgaat gtaaatgacg ttctatatgc aggtcataat agcacatggg accctcaatc
     2281 aaattcaatt gcgaaatata attaccctaa tggtaaacca gaacatttag actatatatt
     2341 tacagataaa gatcataaac aaccaaaaca attagtcaat gaagttgtga ctgaaaaacc
     2401 taagccatgg gatgtatatg cgttcccata ttactacgtt tacaatgatt tttcagatca
     2461 ttacccaatc aaagcctata gtaaatagtg ctcaactaac taataacttg cttcgttcta
     2521 aaaggacgaa gcgagttata ttgttaaaat ttgaattgac ttacatttta ataaaatcat
     2581 cttaacaact ttaatttttc attaatacaa gtctttactc tacactcaaa caagattcat
     2641 acactgcacg tcataataaa tctatctatt caaatataaa taaaagttac ctactacatt
     2701 ctatgtagca ggcaactttt attacttatt tcttttcatt atcattaagt acttttacaa
     2761 acttcacatt atgtgtcttc caatcaactt catataatgc tgataatttt tcttcttttt
     2821 tatctacatg gttttcacca gaccaatagc cccagaaacc atggcgattc caatctattt
     2881 taaactcatc cattgatctt ttataatgaa caacaaattg tgatttacct ttgtcttttt
     2941 tatcatgtga cataacagct aaaaattctg gattaaaccc ttcagacaca gttacaggca
     3001 ttttgtcttt aggtgtgaaa ttatctttcg cccataaatt tccatttcgt gttaaagaaa
     3061 aaatttcact tttagttcta ttatcactat cattagttaa ttgtctcgta tggtcatgtc
     3121 ccatattatt tatcaaatgt gcttctactt tccaacctac acctttatgt gacgtagatt
     3181 gatcaagtaa tgtacgatat gatggttgtt gataactaat cgtctctgaa taattactct
     3241 cttttgtaat atttccagtt aagcctccac gattaatcga aaaatctcca cctgttttat
     3301 aaccatacgt atatttaact tctcttgatt catcctgatt ttttggtgca aagtcagtca
     3361 catttgtatt gttgttgtca tcaacatttt gaattgaaac tgaataagat ccaggccatc
     3421 ttaatgtact attccaataa ccatttgggt ctaaaattct caaaccacta ccaattgtac
     3481 cttttgcttt aataaatact gtttctttat cataattagg ttcagttaag aaattaaatt
     3541 gtaagctttg agtaatattt ttttgactat cacttgttgt agctgtacgt gtatacattt
     3601 tagtatcacc atcaagattc ttctcagaaa cttgtttgat ttcagaatta atctttgcaa
     3661 atgaagtagc tggtaataca gtgaaagtag tcgatagtgc taacgtacaa attgtgatat
     3721 ttttacatag ttgtttaatc attagtaatc cgccctttca atattatcct tctttataag
     3781 gtttattgtc atcagaatat ttatcaacga ctttaactgt tttatttttc caatcaactt
     3841 cataagtgac aattaatctt tgaccatctt tatttttttc taaaattgga ggtgcataat
     3901 gtattccagg tctgtttttc aaaatatctt gatttcgtgt gtatgttact tcaaattgcg
     3961 ttttctcatt tgacttttca ttagataaat aagttaaaaa ttctggatta aagccacttc
     4021 ttactaatgc tgggtatcta tattttgaag caaagcttag ttcagggttt tctacagtag
     4081 caattctcgt atttctatag aataataatt catcatttct atttttcact tctccaccat
     4141 acttcaagtc attcgcaata actgaccagt gtacatgcca gttattattt ttaccgctgg
     4201 caattgtgtc ataattttgc tgattataac taatcgtttt ggagtagcta tttgatgaag
     4261 ttcgtccaat accttttgtt gaatcgaatt taccacctga gctatatgaa aatgtactgt
     4321 ctacttttgc agttgaaatt ttatttttcg gcaattggtc taatatttca gttttacgat
     4381 ttctttttac ttgaaaatct acatggtact cacttggata ctttaaccaa tttgaatttt
     4441 tttcttcttt atgagattca aactttaaat ttgaatgaat tgagccttgt tttttaacaa
     4501 gtaatacatt cttgtcataa gttggatcat cgataaagtc gaattgtaaa ttttggagta
     4561 tatttgtttt ctcatcatat actgtttcag ttcgttttgt gattttaccg tttttcccaa
     4621 tatcatccgg tgctgttgaa tttttatctt tattagtaac attacgtttg tctttttgtt
     4681 gagacttatc aacatgttct ttcttatttt ggtcttgaga gtctttatga gctgaatttg
     4741 cttgagtcgt tgctgctgat aacaataaaa ttgcacatga taatgatgac gctattaaaa
     4801 cacgtttttt atttttcatt gttataacct tctttcgtat gattgatatt tgttgatatg
     4861 tatcgacatg tgaataatat cacaaaaaca gagaatatat atttaactat
//
LOCUS       DAKTLH010000164         4791 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.168, whole genome shotgun sequence.
ACCESSION   DAKTLH010000164 DAKTLH010000000
VERSION     DAKTLH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.168"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(265..2073)
                     /gene="pepF"
                     /locus_tag="O0394_002424"
     CDS             complement(265..2073)
                     /gene="pepF"
                     /locus_tag="O0394_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCY0500481.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLETDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
     gene            complement(2121..3106)
                     /locus_tag="O0394_002425"
                     /pseudo
     CDS             complement(2121..3106)
                     /locus_tag="O0394_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
     gene            complement(3227..3946)
                     /gene="mecA"
                     /locus_tag="O0394_002426"
     CDS             complement(3227..3946)
                     /gene="mecA"
                     /locus_tag="O0394_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCY0500482.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVYFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
     gene            complement(4317..4712)
                     /gene="spxA"
                     /locus_tag="O0394_002427"
     CDS             complement(4317..4712)
                     /gene="spxA"
                     /locus_tag="O0394_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCY0500483.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
BASE COUNT         1517 a          824 c          622 g         1828 t
ORIGIN      
        1 tataaaaagt gcatatttaa tacattttta tacaacttca cttccctctt attaaaaaag
       61 aacataaaaa aacgtcgtgt ctgaatacac gacgctaaac aaaggggatg ggagaaattt
      121 ttcacttcaa acaaaggggt atgtttgtta tgtgattaat ttcatgttca taatataaca
      181 tgctatctct accatttcaa ccttttaata gtacttaatt taaattgtca caatctgttc
      241 ataatgtaag cgaatacaat agtactaagc tttcattaat ttttcaaaag cgttcaattt
      301 ttgttcaaaa acttcacaag cttgttcaat tggttcaggt gttgtcatat ctacaccagc
      361 attttttaat atctcaattg gataatttga gctacctttt ttcaagaatt cattaatata
      421 tctatctact gctggcttac cttctgttaa aatttgatga cttaagcttt gagctgcact
      481 gtaaccagtt gcgtattgat atacataata attcatatag aagtgtggaa tacgtgacca
      541 ttccttacta atatcttcat cagtttctac agaatcaccg aagtataatt tatttaattt
      601 ggcatattct tcattcattc tagttggcgt taatggttca cctgcttctt caattgcatg
      661 aattttatgc tcaaattctg cgaacattgt ttgtcggaat aatgtagctc tgaaacgttc
      721 taattcttgg tttaataata ataagcgttt ttcatcatca agatgtttat ccatataatc
      781 acttaaaagt gcttcgttac aagttgatgc aacttcagcg acaaaaatag tgtagtcact
      841 agaatttgac ggttggaatt ttctactgaa gtaactatgt gctgaatgcc caaattcatg
      901 aactaatgtg tataagtctg aaatagtatt agaccagtta agtagaataa atggattagt
      961 taaatgtgca cctgatgaat agccacctga acgtttacct ttattctcat agacatcgac
     1021 ccaacgattg tttaagcctt ctttaactac atttaaatat tcttcaccca ttggttctaa
     1081 tgcttttaac atccactctt tagcttcttc ataaggcatt tcaaacttaa tatctttaat
     1141 taatggtgta tataaatcat acattttcaa gtcatctaaa cctagcaatt ctttgcgcaa
     1201 ttcagtatat ctatgtagca atggtaaata tttatgtaca gtttttacta gattgtcata
     1261 tacattttct ggaatatgat tattactcaa tgctttttct cttgcagttt tgtaattgtg
     1321 tgtacgagca ttaaatacat ttttcttcac ttcacctgct agcgtagcgc caagcgtatt
     1381 attatgagca ccatatgctt tatatacatt tctaaaagca ctttctctta gtttgcgatc
     1441 atctgtttct aaatacttaa taaatgtacc ttgtgttaac gggtgtgcat ttccatcttt
     1501 atctatcgca tcttcaaata ctaaatcagc gttgctaaac ataccgtata catttgatgg
     1561 cgttgataac gcgtcctgcg cttctgttaa taacttttca gtttcagcat ctaaaatatg
     1621 aggacgtttt tcattaatca acttcaaatc aaatgcgaat ttttgtaatt tatcatatga
     1681 atttacaaat gattgaattt tatcttcatc aatttgtaaa atctctggca ctaagaaact
     1741 ccatgccgag ctaaatttaa taattaattg atgtgctctt gactccatac cagtatactt
     1801 gtcgttcgtt gtatcttggt cttgttttag gtgcgcgtat acatatactt tttctaattt
     1861 agtacctaat gtatcttcta attctaacgc attgtataat gtctcagcac tatcaccaat
     1921 gtgtccttta aattgttctt ctttgcctaa ctcattttca acttctttaa atgcagcctc
     1981 aaaagcttca tcatctttga aaattgttgt taagtcccat gtatattcag gatatttacg
     2041 ttcctgttct tctctcgata attgttgact catatgtagt aaacctccta ataaaatata
     2101 attgatataa ttttctcatt ttagaacaaa atttgcacgt cattaccttt atctgaacaa
     2161 atatttaaat aattgttaat aagtttttct ataattgctg ttttactttc tagagcaaca
     2221 ataaattttt tcagtttaaa ataattaaga ttgtcattta ctaatttgct ttttccattc
     2281 tttaaccata aatcaacttg taattgccac tcaataggat gatttttaat ataaatttgc
     2341 tctggaaaaa tataaccata attgtgcact acttcttgat cagttaactg taattgatac
     2401 attgcactta aagtgggttc taaaacagaa ttttgccagc gacaatattt gatatattga
     2461 ttaattgcga actttgataa tttataaaca gcatgataat cacaaggcgc ctcattaaac
     2521 aactcactaa ttttggcatt tcttttttca gcgacatatt tgtgcccgcc taaatattgt
     2581 atttgttgaa acatcattat ttgttttttc tctaaattga acgtatgaag ttttcgagtg
     2641 aatggattaa taaacatact ttgaaaatga ttgaacttca ctttattatg acaataatca
     2701 acatcattta ataaccaact tactttatat cccattttct ttaaaccttc cgttcgttga
     2761 agaatctgtt tataaggaat tggcgaatac tgtagctcaa taacatattt attattaatc
     2821 aaaatatccg gaatttgttt tatttctttt aaaatggctc tatctctact ttacatcctt
     2881 gttgctgtat catctgtgct aaaatcaatt tacttttata atgtttaatc gtttcattat
     2941 taaaacattt ttgttccgct aaatgtttat gtgcaaaatg actaattact ttgagcccac
     3001 gctttaaaat aacttgcttg ccacacaccg gacaaaaata ttgtgtcttt ctcaatgcag
     3061 tagttgctaa tacgcgttcc ttttcttcat ttaaagctac taacatatct tcacctcaaa
     3121 atattacacg taagtttaac tactattact ttttcagctt ttgaaatcaa taaaaaaaga
     3181 gactccaaag agtctccttt catagtgcgt tgcttaatta atatcgttat tcagttgtct
     3241 ctggaaaata acgtcgaact tgagctgtta cgttatgact cataattatt ttagcatagt
     3301 catttaaata aacttctgtt ctgtctgttg gataagcaaa ttcaagcaat tgactgtaac
     3361 tatcattaat gacttcttga tcaacatgac tatcaaaata tacagcataa taataagtac
     3421 catcaaccat atataacaaa tcttcaaact ctgtagttat tggattgcta tgatatgcat
     3481 aattaataac atcttctaaa tcgttaaatt tcacaatgat tgttcttgta tttttacgtg
     3541 ctgaagactt ttgacgttta gaaccttgag cttctttttc ttttgttcgt tgctcgaata
     3601 attcttctaa ttgatcttca ccttctaatg tttgagctaa caattcttga acttgttcat
     3661 caaattgatc agttgcatca tcatcagaca tattcatcat atcttcattt ttagatttag
     3721 aaattgtgac ttcgacacct ttttcaaagg catgtacttg aatccataat ggaccttcta
     3781 caacaaaatc ttcttcttcg ttaatttcat ccatcattga ccaaaagaat tcttcgccac
     3841 gtttgcgatt tgtccataaa tcttcacgac taaatccacg ggcctcgata tcgctatatg
     3901 ttataaacaa ttttacagtt gtatcatcta ctcgttctat tctcatatca tctcactcct
     3961 tacagtcgat gaatagtatc cacattgtat taaagcatca aacaaaatac aatttatttg
     4021 tttgatttct ttctctaaaa tattttggta acttataata acatattcgt tgtagcaaaa
     4081 ctatttaaat gatttataca gttaaagcat taaagcactt tcttaattta attttacctt
     4141 atcatagcaa atgtttcatc caaaacactc taatatagag ctatattaac aattgcacat
     4201 ttgatttaaa agatactaag caattattgt aaaattaaaa ccaaaaaagc aacactatag
     4261 aaatagtgtt gcttgacata tcttttagta aagtatgaca catagttaaa tggttattag
     4321 tcaaccatac gttgtgcttc ttgtaattgg aacgtacgaa cttttctagg taagaaacgt
     4381 cgaatctcgt cctcattata accaacttgt agtcgtttat tatctaaaat aattggacga
     4441 cgtaataagc caggattatc ttgaatgatt gaatataagt cttgtaatgg tagtgaatca
     4501 atatcaacat ttaatttttg gtatgtttta gaacgtgtag aaatgatttc atcagtaccg
     4561 tcttcagtca tttttaatat ttgcttaatt tcatcaattg ttaaatgttc agaaaaaata
     4621 ttacgctccg tatacggaat gtcatgttct tgtaaccatg ctttcgcttt acggcaagat
     4681 gtgcaacttg gtgaagtaaa taatgttacc atacatctca ctctcctatt tgaatgaata
     4741 aaattcattg cttaaaattt agttatagat caagaaaaaa catttttttc t
//
LOCUS       DAKTLH010000165         4788 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.169, whole genome shotgun sequence.
ACCESSION   DAKTLH010000165 DAKTLH010000000
VERSION     DAKTLH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.169"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..338)
                     /gene="deoC"
                     /locus_tag="O0394_002428"
     CDS             complement(<1..338)
                     /gene="deoC"
                     /locus_tag="O0394_002428"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCY0500484.1"
                     /translation="MNSAKLIDHTLLKPESTRTQIDQIIDEAKAYHFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVV"
     gene            653..1363
                     /gene="deoD"
                     /locus_tag="O0394_002429"
     CDS             653..1363
                     /gene="deoD"
                     /locus_tag="O0394_002429"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCY0500485.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            complement(1484..1927)
                     /locus_tag="O0394_002430"
     CDS             complement(1484..1927)
                     /locus_tag="O0394_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCY0500486.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(2127..2540)
                     /locus_tag="O0394_002431"
     CDS             complement(2127..2540)
                     /locus_tag="O0394_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HCY0500487.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
     gene            complement(2998..4371)
                     /locus_tag="O0394_002432"
     CDS             complement(2998..4371)
                     /locus_tag="O0394_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HCY0500488.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRISNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
BASE COUNT         1484 a          847 c          663 g         1794 t
ORIGIN      
        1 accactgctt caatgtcttg ttgtacatca tcaaaacgtc catcttttaa tgcgccgatg
       61 ttgatgacca tgtcaatttc atctgcacca ttttgaatcg catcttctgt ttcaaatgct
      121 ttcgtcgcag ttgtcgatgc acctaatggg aatcctatta ccgtacaaac taacacctct
      181 gaatcagcta gtcgctctgc tgcatattta acatgcgttg gattcacaca tacagattta
      241 aaatggtatg ctttcgcttc atcgatgatt tgatcgattt gcgtacgtgt tgactcaggc
      301 ttcaataaag tgtgatcaat caattttgca ctattcattt tctatctcct cctttatggt
      361 tgattataaa aatacggttg taaattagtt gattgagcgt caggttcatt taaatatcag
      421 gttagatgtt cgctttttat gtaaccgcat acatatacta ttacattaat tcatttccca
      481 taaacaaaca atacaattga acgtgatatc ttcattatga acgatgactt gacaacaagc
      541 taatcaggat tatattttta taattcttta attctatagt acaaaaattc gcaaaaaagg
      601 gaaacaaatg ttatcttaaa attattaatg aatattaagg agaagataac aaatgacaaa
      661 aggtacacca catattcaac caaatggagt aaaaattgct aaaacagtat taatgcctgg
      721 cgatccgcta cgtgcaaaat atattgctga taatttttta gaaaatgttg aacaatttaa
      781 cgatgtacgt aacatgtttg gttacactgg tacatataaa ggtaaagaag tttctgtaat
      841 gggttctggt atgggtattc caagtattgg tatttactca tatgagttat acaacttctt
      901 tgatgtagat acaatcattc gtatcggttc ttgtggcgca ttacaagaaa atgttaactt
      961 atacgatgtt attattgcac aagctgcatc aactaattca aattatgtag atcaatacaa
     1021 tattccaggt catttcgcgc ctatcgctga cttcgagtta gtaactaaag ctaaaaatgt
     1081 cgctgaccaa atcggtgcta ctacacacgt aggtaacgta ttatcttctg atacatttta
     1141 caatgccgat ccaacattca atgatgcttg gaaaaaaatg ggtattttag gtatcgaaat
     1201 ggaatcagct ggtttatatt taaatgcgat tcatgctggt aaaaaagcac ttggtatttt
     1261 cacagtaagt gatcatattt tacgtgacga agctactaca cctgaagaac gtcaaaattc
     1321 atttacacaa atgatggaaa tcgctttaga aatcgcagag taacttattt aaattgactt
     1381 taattgctct ttaacaatgc gattaaactc aaaaagccaa cacattctgg gcgtatcccc
     1441 atttatgtgt tggcttttat ttatattatt acttatctgt agattagctt aagtaagatt
     1501 taaacatcca attatgttta tctactgatg tttgcatacc tataaacata tcttctgata
     1561 catcatcgcc agcattacca gcaatttcga ttgcgttttc taattgtttt gagatatttg
     1621 tgaagtcttg tgataattct tcaaccattt gttctgcaga gtaacctttc gcagcttctt
     1681 taacaattga ttgctctaag cattcagtta atgtacctac agggtttcct cctaccgcta
     1741 aaattctttc agctaattcg tctacatatt ggcttgcttc attatataat tcttcaaatt
     1801 taacgtgtaa tgagaagaag ttaggccctt tcacatacca gtggaaattg tgtagctttg
     1861 tgtaagctac tgtccagttt gctacttgtt gattcaattc ttttacaaca tcttgttgat
     1921 tactcatatt taatacactc cttaaaattg tctacgtctt gcttgtttat attaattatt
     1981 atattataat aattctaatc tgtaaagtgt ttttgctctc ttttttataa ttattctaat
     2041 ctaaccgtaa agtgttccgc tatttaaatg cctaaatttt aattcatcat ctaatatatc
     2101 cattatcaat tagcaagtat gttttatcaa tcagtaagtt ttactttcgc attatcatct
     2161 aaaaagaacg atttttcata atcatttggt tggtgccatg ttgttttcca cattctattt
     2221 ctattattag caaacattcg ataaccgaaa ttacggactg gtttaggtac aagccataaa
     2281 cctacgccca ataatttagt tcgattaggt agtgctgtga ttagtttaat aatcgcctgc
     2341 gattcaaaat atatacgatc acctttctgt aaaatcacac tatttttatt tgccgcttct
     2401 ggatgttgtt taaaaaattg ttggccaact tcacctttta acgtggcaaa ttgataattt
     2461 cttggtaggt catgttgaat taaccaaatg acatagttat aacaatatat acagtttcca
     2521 tcataatata cgattggcat aatttattcg ctcctttaat agtacttata gtcatttttc
     2581 ctaatttaaa aacatttaaa caaggttgtc acttgataag tcctcgcgac acgaaattgc
     2641 cataaaattt atttttcagt tttatattta ttgtataatg tattttttat ttactttaaa
     2701 tttctcaatc tacagtgtaa tcttatttct tattattaac ttagcgtttg gtcattcgtc
     2761 ttattcaaca taccactgga aaactcatat aaagttttaa ttctgtaaca taaattcttt
     2821 tatattcata tactgtcttt attatttatt aaaataaaaa cgagtaatct atcataagct
     2881 cttaattagc tcttatggtt gattacccgt cattttttct ttatgcggtt attcaaatat
     2941 gcaattatgc aatttttaat cctatcctta cttcttttca aaaactacca tatatcatta
     3001 gtcatgtttg actttgacta tacgtgtgta ttttaaaaac tgctctgaat atttctcctt
     3061 aatttcctct aaactgtcat atgtaatacc tacaatatgc caattaggat tgaaataata
     3121 gaaagaatct ttttgtcgtg accatttaat catattaccg tctttattat aacgtggtaa
     3181 tgttacttca tagccttcta acacattaat atacgtttgg aaaatgtctg tatctatacg
     3241 attaaaatta tcaataacta aataactttt atccttttta ggcattaatt gagtaataaa
     3301 cccttcacga tagtataatg cccctgtttc atttggtaaa tacttgccat aaagcatatc
     3361 ttctgaaaaa tcaggatgac ctgttgttat aacaggattt gcattcgctt tttgcaataa
     3421 gtcattagcc gcttttaagc cttcttcttt tcgatctaca acaagtaaaa taaatggctt
     3481 taacgcttct tctttttcac tttcaagcat atctggtttt tgaaccattt caaatggaga
     3541 tttcaatcca ttattatcgc tcatttcaat aattgcatca tactgtgctt gtgacatact
     3601 tgcaatagcc tgttttgcat tttcttgaag gaaatataag tttttcaatt taggatgctt
     3661 atttaatgta cttaatgtaa tcggtgtaat gtctttctca taagacactt caatcactgt
     3721 actatttgtt ctaccaggaa ttggtggttt ttcatgaata tgctttgata cttctccaat
     3781 tccaacgaca gattgatttt tcgttcgatt ataaaaaata atattgtcgc cttcttctaa
     3841 ctgagtataa aaatgataac cattacgttt aataccgttg tacgtgtgcg tataaatcgt
     3901 atattggttt ccaggttcaa attcttcagt ttcagctaaa aagaaataac gcggtatctt
     3961 aatttcgcct ttaccaagac cacttattaa atcaaactct tctgcagtga tttgattgaa
     4021 caatgtctct ttcatattac ttatacgaaa ttccaaagct tcactacgct ttaaataatc
     4081 tgctgttaat ggtttcaatt gttcattaaa acgaaactgt acacgtattt tattttgtgc
     4141 acctgtttca acactaataa tttcaccaca tccaagtagt ccagtatccg tctgaacttg
     4201 ataaaagatg acttgatctc ctactttagc ctttttaaac gctctaaatc cttgagatgg
     4261 gttaaaatgt gcgcctgatt caaataaagc tgtttgtcct actaacggtt cattatgatt
     4321 ccaacggtta tatccacaat tcaaccaaaa ataattcgtt tctgctgtca tcttaatact
     4381 ccttaacctg aataaatttt agaaacacta tgaattacat tcttttagtg tttcttatgc
     4441 agttggacgc gtatgcgaac aactgtatac cctttgttca ctgcgatttt aatcgcattt
     4501 cctataacat tgtagcgccc aggacattaa tttacgtccc agacccttat cgttttcact
     4561 tctaagtaag tcgaactatt ttgctttaca acaagtgcga ctctaaatac agttggacac
     4621 acatacgagc aactgtatac cttttaatca gttttctata ttttatttat tatatctgtc
     4681 ttaatgataa aaattgttac aaacagttta acatatttag ctacctattt tgcatataaa
     4741 aaacatgctt gcatacacta tgcaataaaa attacaaatt tatatatg
//
LOCUS       DAKTLH010000166         4783 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.170, whole genome shotgun sequence.
ACCESSION   DAKTLH010000166 DAKTLH010000000
VERSION     DAKTLH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.170"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            21..773
                     /gene="sarS"
                     /locus_tag="O0394_002433"
     CDS             21..773
                     /gene="sarS"
                     /locus_tag="O0394_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HCY0500489.1"
                     /translation="MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLF
                     HQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQRE
                     KIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVE
                     FTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILI
                     HMDDAQQDHAEQLLAQVNQLLADKDHLHLVFE"
     gene            1194..2720
                     /gene="spa"
                     /locus_tag="O0394_002434"
     CDS             1194..2720
                     /gene="spa"
                     /locus_tag="O0394_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HCY0500490.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKP
                     GKEDGNKPGKEDNNKPGKEDNNKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDNKKPGK
                     EDGNKPGKEDGNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIK
                     PGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL
                     "
     gene            complement(3049..4641)
                     /locus_tag="O0394_002435"
     CDS             complement(3049..4641)
                     /locus_tag="O0394_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCY0500491.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISVDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
BASE COUNT         1821 a          881 c          703 g         1378 t
ORIGIN      
        1 atatacaagg agataaaaca atgaaatata ataaccatga caaaattaga gattttataa
       61 tcattgaagc atatatgttt cgttttaaga aaaaagtcaa gcctgaagtc gatatgacta
      121 taaaagaatt tatattactg acttatttat ttcatcaaca agaaaacaca cttccattta
      181 agaagattgt ttcagattta tgttataaac aatcggattt agtacagcat ataaaagtac
      241 ttgtgaaaca ttcatatatt agtaaagttc gaagtaaaat tgatgagcgt aatacttaca
      301 tttcaatatc tgaagaacaa cgagagaaaa ttgcagaacg tgttacattg tttgatcaaa
      361 tcattaaaca atttaacctt gcagatcaaa gtgaatcaca gatgatacca aaagatagta
      421 aagaatttct aaacttgatg atgtatacaa tgtatttcaa gaatattatc aaaaaacatc
      481 taacattaag ttttgtagaa ttcacaattc tagctattat cacttctcaa aataaaaaca
      541 tcgttcttct taaagattta attgaaacaa tccaccataa ataccctcaa actgttagag
      601 ctctcaataa tttaaaaaag caaggctatc taataaaaga acgctcaact gaagatgaaa
      661 gaaaaatttt aattcatatg gatgacgcgc agcaagacca tgctgaacaa ttattagctc
      721 aagtgaatca attattagca gataaagatc atttacatct tgtttttgaa taatatctct
      781 attacgcaag tgtgctgtat tctaaagtgc acttgtgttt tctatttttt aataaaacct
      841 cagcacataa tgaacaactt tctattttct atatcactta aaaccatttc cgaaattaaa
      901 cctcagcaca ttcaaagccc cactttattc ttaaaaatat tttttaactc atatgtatta
      961 aaccgctttc attataaaaa atatctctat attttatctg tttttattaa tcgaaatagc
     1021 gtgattttgc ggttttaagc cttttacttc ctgaataaat ctttcagcaa aatatttatt
     1081 ttataagttg taaaacttac ctttaaattt aattataaat atagatttta gtattgcaat
     1141 acataattcg ttatattatg atgactttac aaatacatac agggggtatt aatttgaaaa
     1201 agaaaaacat ttattcaatt cgtaaactag gtgtaggtat tgcatctgta actttaggta
     1261 cattacttat atctggtggc gtaacacctg ctgcaaatgc tgcgcaacac gatgaagctc
     1321 aacaaaatgc tttttatcaa gtcttaaata tgcctaactt aaatgctgat caacgcaatg
     1381 gttttatcca aagccttaaa gatgatccaa gccaaagtgc taacgtttta ggtgaagctc
     1441 aaaaacttaa tgactctcaa gctccaaaag ctgatgcgca acaaaataac ttcaacaaag
     1501 atcaacaaag cgccttctat gaaatcttga acatgcctaa cttaaacgaa gcgcaacgta
     1561 acggcttcat tcaaagtctt aaagacgacc caagccaaag cactaacgtt ttaggtgaag
     1621 ctaaaaaatt aaacgaatct caagcaccga aagctgataa caatttcaac aaagaacaac
     1681 aaaatgcttt ctatgaaatc ttgaatatgc ctaacttaaa cgaagaacaa cgcaatggtt
     1741 tcatccaaag cttaaaagat gacccaagcc aaagtgctaa cctattgtca gaagctaaaa
     1801 agttaaatga atctcaagca ccgaaagcgg ataacaaatt caacaaagaa caacaaaatg
     1861 ctttctatga aatcttacat ttacctaact taaacgaaga acaacgcaat ggtttcatcc
     1921 aaagcctaaa agatgaccca agccaaagcg ctaacctttt agcagaagct aaaaagctaa
     1981 atgatgctca agcaccaaaa gctgacaaca aattcaacaa agaacaacaa aatgctttct
     2041 atgaaatttt acatttacct aacttaactg aagaacaacg taacggcttc atccaaagcc
     2101 ttaaagacga tccttcagtg agcaaagaaa ttttagcaga agctaaaaag ctaaacgatg
     2161 ctcaagcacc aaaagaggaa gacaataaca agcctggcaa agaagacggc aacaagcctg
     2221 gcaaagaaga caacaacaag cctggtaaag aagacaacaa caagcctggc aaagaagacg
     2281 gcaacaagcc tggtaaagaa gacaacaaaa aacctggtaa agaagatggc aacaagcctg
     2341 gtaaagaaga caacaaaaaa cctggtaaag aagacggcaa caagcctggc aaagaagatg
     2401 gcaacaaacc tggtaaagaa gatggtaacg gagtacatgt cgttaaacct ggtgatacag
     2461 taaatgacat tgcaaaagca aacggcacta ctgctgacaa aattgctgca gataacaaat
     2521 tagctgataa aaacatgatc aaacctggtc aagaacttgt tgttgataag aagcaaccag
     2581 caaaccatgc agatgctaac aaagctcaag cattaccaga aactggtgaa gaaaatccat
     2641 tcatcggtac aactgtattt ggtggattat cattagcctt aggtgcagcg ttattagctg
     2701 gacgtcgtcg cgaactataa aaacaaacaa tacacaacga tagatatcat tttatccaaa
     2761 ccaattttaa cttatatacg ttgattaaca cattcttatt tgaaatgata agaatcatct
     2821 aaatgcacga gcaacatctt ttgttgctca gtgcattttt tattttactt acttttctaa
     2881 acaacttctg aaacgcctca acactttcta ctctgattac atatatgaca tttttaggca
     2941 ttaaaaaatc gaactagaca agatgctcat tgcatttcgt actagttcga ttcatgaata
     3001 attagattta aaatgtcatt tgaatccaag tgacaacatt atttatattt agaatattaa
     3061 cgttagtata aacgtccaaa cacaaataaa agcaacaaat ataatactgt attttaacgt
     3121 catttttaat aatgcagatt cttcaccaac ttttttaaca gctgcagtcg caatggcaat
     3181 tgattgtggt gaaataagtt tcgctgctac accacctgca gtgttagctg ccacaagtaa
     3241 tgaaccgctt gttgaaattt gttgtgccac tgtcgcttga ataggtgcga ataaagtatt
     3301 gttatttaca actgaaccag tcataaacac accaatccaa cctaatactg gagagaataa
     3361 tgggaaaatt gctcccgctt tagcaatacc ttgtccaatt gctacagtca aaccaccgta
     3421 tgtcataact ttagcaatag ctaggatagc tgaaattgta aggatcggta accataattc
     3481 tttaattgct tcgaccaata aagcacctgc acttttccat tttaacttcg taattaaaat
     3541 tgtaataatt actgttaata aaatcgctgt cccagttgca ccaattaaat cgagacgcaa
     3601 cgcaattcct ttaggcgata aatcactcac agtatttgga attggcaatt ttattactaa
     3661 actttcaagt gcacctccag gttggaataa ttttttgaag aatggtgcac tccatactaa
     3721 tacaaaggca gttaaaatta cgaacggact ccaagcaaag acaatttctt taggcgttcg
     3781 tttttgaatt ttatgttcag acgcttccaa tctgaaaatg tttttcggtt taaatttacg
     3841 acaaacaaat gctaacacca ccattgttgc tagtgatgga ataatgtctg ctagttctgg
     3901 accatggaat attgttaata ataattgtaa tccagtatat gtaccactca ctgttaaaat
     3961 gacaggtaaa atttctttaa tacctttcat accatctaca atgaatacta aaacaaatgg
     4021 aataataaag tttaaaattg gaagtgttaa tgctgagtat ctcgcaacat ctaatgttgt
     4081 aacgcctcca cttaagttaa acgtatcaat aatactaact ggtaaaccaa ttgcaccaaa
     4141 ggcacccgcc gcaccattag caattaaaca taacatcgct gcttttaatg gttcaaatcc
     4201 aagttgaatt aataatactg cacaaatcgc aattggcaca ccaaatcctg ctgcaccttc
     4261 taaaaatgcg ttgaaacaaa atccaattaa taatagttgg attctttggt ccactgaaat
     4321 acttgcaata ctatcttgaa taatagaaaa ttgtcctgtt ttaatagaaa ctttatataa
     4381 ccaaactgcc attaaaacga tatatcctat tgggaaaata ccggcaacaa cgccttctgt
     4441 aatcgcacct gctgatacac gcgctggtaa ttcaaataca aataaagcca caatcaatgt
     4501 aacaaccaaa gttgtcaatg ctgcataaat gcctttcatt ttaaaaacgg ttaagcataa
     4561 taaaaataaa ataataggta ctgctgcaac taaggctgat aatccgacat tatcgaatgg
     4621 atttacagta agtagtgtca taatgactcc ctctctttat ataaaatatt tatcattctg
     4681 attaatctac aacctatttc aacttatatt ttgcgatgat cacatattta aaatgtaaca
     4741 ctcctatatg tgacaggcaa tcgaattttt acaaaaagtt cac
//
LOCUS       DAKTLH010000167         4757 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.171, whole genome shotgun sequence.
ACCESSION   DAKTLH010000167 DAKTLH010000000
VERSION     DAKTLH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.171"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            27..212
                     /locus_tag="O0394_002436"
     CDS             27..212
                     /locus_tag="O0394_002436"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCY0500492.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            complement(300..1283)
                     /locus_tag="O0394_002437"
     CDS             complement(300..1283)
                     /locus_tag="O0394_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCY0500493.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            1420..1929
                     /gene="msrA"
                     /locus_tag="O0394_002438"
     CDS             1420..1929
                     /gene="msrA"
                     /locus_tag="O0394_002438"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCY0500494.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            complement(2132..4654)
                     /gene="mprF"
                     /locus_tag="O0394_002439"
     CDS             complement(2132..4654)
                     /gene="mprF"
                     /locus_tag="O0394_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCY0500495.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVAITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILD
                     KITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYK
                     QSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRN
                     IVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAW
                     LFDYQFSKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKAS
                     SLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGEFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
BASE COUNT         1732 a          838 c          668 g         1519 t
ORIGIN      
        1 ttaataatga ttaggaggtt tggatgatgc caatcgtcaa tgtaaaatta ttagaaggtc
       61 gttcggatga acaattaaaa aatttagtta gcgaagtaac tgacgccgta gaaaaaacaa
      121 cgggggcaaa tagacaagca attcacgttg ttatagaaga aatgaaacca aaccattatg
      181 gtgtggctgg cgtaagaaag tcagatcaat aattcttcat aagatgcatg ccaattaatt
      241 ctttgaaaac gaacaaggcg acttctatct gagtatgata gaaatcgcct tgtttatttt
      301 taatcttcat ctaaaaagtc tttaatagct tgtttatttg tgtttttatt aatctgtaat
      361 gcactaccat cagtatttgt attgacatct tcgtatgagt tcttgattgg cacagtcaat
      421 gacttaacat ctttttcacc tcggatacca aaactcaaac ctgtttggaa aatccctgaa
      481 tcaggaatgt ttgtattcac atagcctctt aaaatacctg caacttttgg taatttaaca
      541 actgttctaa aattaaccat ttcttttttc aatgtttgca tcacttgttg ctgacgtcgc
      601 acgcgtccga agtcaccttc agggtcgtga cggaatcttg cataaccaag taattcttta
      661 ccattcaacc tatggttacc ctttttcaaa gatacaccaa tatttttcga catatctttt
      721 tcgacattaa ttggtacacc ttcaggcatt aattcatcaa tcattttctc aaatccagta
      781 aaatcaacta ctgcataata ttcaggatta attcctaaat ttttatcaag tgtttttcta
      841 agtagctctg gaccacctaa agcgtatgct gaattaattt tgtgttttcc atatcctgga
      901 atatctgcat aaatatcacg catgacagac atcattttca tctttttatt gataaagtca
      961 tattgaacaa ccatgataga atctgttctt gattgtccac cttgtgcttt atctgcaccg
     1021 agtacaagaa tagaaatttt accatcattt tttactggtc cattaaattg atgtacttta
     1081 acatctttcg catgtttctt ggcatattct acaccgctat tgtaactatg tacaatatat
     1141 acaactaatg cgataagtaa aattacaaca atcagaagaa tgataggtaa tttcctaatt
     1201 ttcttcttct tttttcgctt aggcgccgaa gtgcgatgtt cactttgacg tctatattcg
     1261 ttgtcattag tttctttatc catatttacc taccttatat cttcaaaaat agatttttgt
     1321 gtactataat aatcacatat taacgttata taaaaacaat tcaaaaagca agtgatttct
     1381 taataaatcg tactaaggtc taatgaaagg atgtcaaaaa tgaatattaa tacagcttat
     1441 tttgccggag gttgcttttg gtgtatgacg aaaccatttg acacctttga cggcatagaa
     1501 aaagtaactt ctggatatat gggcggacat attgaaaatc ctacttacga acaagtaaaa
     1561 tcaggtacga gtggtcattt agaaactgtt gaaattcaat atgatgttgc attattctca
     1621 tacaataagt tattagaaat atttttctca gtcattgacc cattagatac aggtggtcaa
     1681 tatcaagacc gtggtcctca atatcaaaca gctattttct acactaatga tcatcaaaaa
     1741 gaactcgctg agacttatat cgagcagctt aaaaatacga ttaatgctga taaggcaatt
     1801 gcaacaaaaa tattaccagc gtcacaattt tacaaagccg aagactatca ccaagatttt
     1861 tataagaaaa atccagagcg ctatgcagaa gaacaaaaaa tacgccaaga atacaaaaat
     1921 aagcaataac atttatactt gatgaaactg attggactga taataaaagt taggcgactt
     1981 aactttagct catttcaata ttaaaatacg gtacagatag gaaacttaaa tattcttatc
     2041 tgtaccgttt tttataaatc attctttata gaaaccaaaa aatccaagcg catcaggcat
     2101 aactgtatac ctcttagcac ttggatttta attatttgtg acgtattaca cgcattactt
     2161 tagaaagtga ttcccaaagc gaattatctt tacgataaac taaaaagcgt ggttcccaat
     2221 tcggattata tttagattta taacgacgta atccttggaa acgatataga ccgttgaaat
     2281 gttcaaagac acggcctgca agtcgttctc ttaaatatga ataatgcaat tgaccaacgt
     2341 tcgataacgt tgccataccc atattaaatt ttgtataacc ttgttcttta ctccaaagta
     2401 acatatgcaa gtatagacca tccattaatg gtaaatctaa ctctggcaac catctaatta
     2461 aatcgactga aatggcatca ttaaagtatg ttggcattaa actacaaaat gcaattactt
     2521 cattttcttc atttcgcatt acaccaattg gcgctttaga taagtattct tcattaaatt
     2581 caccaacaga gaaatgcatt tcctgacgat tatctagcca taaatcactt acatgttgaa
     2641 gttcatttat aaattcagtt gaaaacggtg gttcaataat ttcgaacgaa atattaagtt
     2701 catcgaattt atttaaagtc gctctaaatc cacggcgttt tttacctgaa gttgaaaatt
     2761 gcgttaaatc aataattgct tcttcaccta atttgaaaaa ttggttaccg aaattatgat
     2821 ataaaggcat gtgttgatct gtaacttgat agaatataac atcatagcct aaatactcag
     2881 cgtaattata gaatgcttct aacaattcat caaaggcatt ttcatcacct aacggatctc
     2941 caagaaccac taatgaactt gctttataac gatacattaa aaatgctgtt ttattttcat
     3001 tagtgaaaaa ctgcttgtca ccactatata tcaagtgact caaataatta ccgccgtact
     3061 gattaataat ctcctcgcaa tcttcaattt tagaagaaat acgtactttg ctaaattgat
     3121 aatcaaacaa ccatgcaatc atacctatga tgatagcgat gattaaaatc gtaagccaga
     3181 agtaatagcg caatacagat gtatgcattt caatcgtata aatatctaat gcatataacg
     3241 ttccagcaat aaatatatgg ttaacatata aaataaataa actgaataaa agcattgcaa
     3301 ctatatttct cattcttact gggcgtttca acctacgtgc tctacggaaa gctacaataa
     3361 gcagaacaaa aataatagct aaccatgtta ttaaaatata tgaagcgtaa gtgaagaatg
     3421 tcgccactgt gattaataaa attgaaatca tagcaaagat aatggcacgt ctactttgct
     3481 tataaatacc aactacattc agtaaaagta ataaacaagc actagtatga attgccaata
     3541 gaatataata cgttaagtga tttccatcat ataaagcatc gtaaacaatc gttaagttat
     3601 ttacaaaaaa gatcatactt gtaaagaata ctaaaattgc taatgataat gatggaattt
     3661 tagcaataat atccttttga taagacatta aaaatgacgt aacatcttta gcaggaataa
     3721 agtatttaga tccctcaatg tacttcttag ctgatgtacc aaattcaaat gatgataaaa
     3781 ttaatgcaat aattaccggt acaaaatagt acgcaaaacg atatagaagt agcattaata
     3841 atactttttc ctcagggaca cctaaagttt taaatcctag taatacaact aaatcgaaag
     3901 cgccgaaacc accaggaata aagctgacta aacctgataa tgcagcaatg ataaatattg
     3961 caataaagga catgaatgat acatgagcgt caacaattac accacagaaa tataatacaa
     4021 ctgcagctgc taaccattca acacacgaca ctaaagtgca gtacaatcct acaaaacgat
     4081 tgtttttatc gggtggtcta accattgaat aaataatgaa taatggtaag aaaaatgaca
     4141 ctacatataa tacccatctt acccatgtaa tcttatctaa gattaaagat gcatcgaaaa
     4201 catggaatac aattagcaat gataataagc ttaaacctgt caacattgaa ataagtatta
     4261 aggatataaa gtgaactaat ttctttttat catgcgtata gtttttataa accattgctc
     4321 taacgcctgc accaataaag ccaccgaaac ctacaatcgc attcaatgca ttgatgatat
     4381 aacttactct taaaactttg cctaaggata tatccatttt taaagctcta gataaaatca
     4441 catcatacat tgatagaata acaagcgatg ccccaccacc aataaatagt aacactaagg
     4501 acatacggtt aatcttacta aattcaacca acgtatcttt aaagttaata ccagataact
     4561 cccgatacaa tgtgattgct acaaaaataa ataaagctgt agcaaacgta atttttaaga
     4621 ttgaaaatat tttgttttta acttcctgat tcattttttc acatcaattc taattatttc
     4681 tgttataaat caaaatatat catataaacc ataaaaatag catatcattt acctttgatt
     4741 tatataacac ttttgtc
//
LOCUS       DAKTLH010000168         4676 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.172, whole genome shotgun sequence.
ACCESSION   DAKTLH010000168 DAKTLH010000000
VERSION     DAKTLH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.172"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..768
                     /gene="lukF-PV"
                     /locus_tag="O0394_002440"
     CDS             <1..768
                     /gene="lukF-PV"
                     /locus_tag="O0394_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_088621906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     F"
                     /protein_id="HCY0500496.1"
                     /translation="DKDTLILKAAGNIYSGYTKPNPKDTISSQFYWGSKYNISINSDS
                     NDSVNVVDYAPKNQNEEFQVQQTVGYSYGGDINISNGLSGGGNGSKSFSETINYKQES
                     YRTSLDKRTNFKKIGWDVEAHKIMNNGWGPYGRDSYHSTYGNEMFLGSRQSNLNAGQN
                     FLEYHKMPVLSRGNFNPEFIGVLSRKQNAAKKSKITVTYQREMDRYTNFWNQLHWIGN
                     NYKDENRATHTSIYEVDWENHTVKLIDTQSKEKNPMS"
     gene            1017..1202
                     /locus_tag="O0394_002441"
     CDS             1017..1202
                     /locus_tag="O0394_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500497.1"
                     /translation="MFILNTQAHARLDQMAQQFEVVCNGLNENEGQAIQTMDQSASLI
                     RSNLIQVKEQLEKLAVY"
     gene            1293..2210
                     /locus_tag="O0394_002442"
     CDS             1293..2210
                     /locus_tag="O0394_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCY0500498.1"
                     /translation="MFKRTKLILIATLLLSGCSTTNNESNKETKSVPEEMEASKYVGQ
                     GFQPPAEKDVVEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGKGVEVFVHCDDH
                     DIVFNASIPFDKSIIESDSSLRSEDKGDDMSTLVGTVLSGFEYRTQKEKYDNLYKFFK
                     DNEEKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIHKNDKEFKE
                     GMEQARKEVNYAANTDTVTTLFSTKENFTKDNTVDDVIELSDKLYNFKNKPEKSTITI
                     QIGKPTINTKKAFYDDNDPIEYGVYRKDE"
     gene            2267..3205
                     /locus_tag="O0394_002443"
     CDS             2267..3205
                     /locus_tag="O0394_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCY0500499.1"
                     /translation="MFKKAKLILIATLLLSGCSAMNNESKKDTNTETNTETKSVPEEM
                     EASKYVGQGFQPPAEKDAIEFVKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVE
                     VYVHCEDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKY
                     DNLYKFLKENEKQYQYTGFTKEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIH
                     KNDKEFKEGMQRARKELNYTANTNTVATLFSTNDERNRKEKINNVIDLSEKIERTKDM
                     PIKNTITTQLGNKLIGTKKARFDDKKVVSFGAFEDE"
     gene            3198..3404
                     /locus_tag="O0394_002444"
     CDS             3198..3404
                     /locus_tag="O0394_002444"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500500.1"
                     /translation="MNKINDRDLTELSSYRVYQDINKDNDFTVNEKRFKQADVFEDLY
                     REKLKDTNKLREYNYLQNETFKSA"
     gene            3419..4333
                     /locus_tag="O0394_002445"
     CDS             3419..4333
                     /locus_tag="O0394_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCY0500501.1"
                     /translation="MLKKAKFILMATILLSGCSTTNNESNKETKSVPEEMDASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVIGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIDSDSSLRSKDKGDDMSTLVGAVLSGFEYRAQKEKYDKLYKFFK
                     DNEEKYQYTGFTKEAINKTQNSGYENEYFYISAIPYNLAEYRDYFEPLLNKSDSEFSK
                     ELSNVKKQLKDKSKVSVTTTLFSKKKNYTKKSNSENVIKMAEEIKKDKEIPNGIELSI
                     KFSDNKINTVKPNFNGESTSEYGVFDQE"
     gene            4532..4651
                     /locus_tag="O0394_002446"
     CDS             4532..4651
                     /locus_tag="O0394_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500502.1"
                     /translation="MTIFTQLSDRIKKAISKINQANNIPNNARKSPMYHSPIT"
BASE COUNT         1960 a          583 c          810 g         1323 t
ORIGIN      
        1 gataaagata cattaatact caaagctgct ggaaacattt attctggcta tacaaagcca
       61 aatccaaaag acactattag ttctcaattt tattggggtt ctaagtacaa catttcaatt
      121 aattcagatt ctaatgactc agtaaacgtt gtagattatg cacctaaaaa tcaaaatgaa
      181 gaatttcaag tacaacaaac ggtaggttat tcttatggtg gagatattaa tatctctaac
      241 ggcttatcag gtggaggtaa tggttcaaaa tctttttcag agacaattaa ctataaacaa
      301 gaaagctata gaactagctt agataaaaga actaatttca aaaaaattgg ttgggatgtt
      361 gaagcacata aaattatgaa taatggttgg ggaccatatg gcagagatag ttatcattca
      421 acttatggta atgaaatgtt tttaggctca agacaaagca acttaaatgc tggacaaaac
      481 ttcttggaat atcacaaaat gccagtgtta tccagaggta acttcaatcc agaatttatt
      541 ggtgtcctat ctcgaaaaca aaacgctgca aaaaaatcga aaattactgt tacttatcaa
      601 agagaaatgg atagatatac aaacttttgg aatcaacttc actggatagg taataattat
      661 aaagatgaaa atagagcaac tcatacatca atttatgaag ttgattggga aaatcataca
      721 gttaaattaa tagatactca atctaaggaa aaaaatccta tgagctaaac agatagataa
      781 tcaaaaaatc ttaaatatgt taaaatttac aaacactttc tttctatatt agggtaacca
      841 cgtcttaatt gacgtggtta ttttttcagg gcaaaaaaag ggcggattat ttaaataagg
      901 acaaacactt gtggaaaatt taaaaggtta aaaataataa agaacttggt ataacaaggg
      961 ttttatacat ttgcgtacaa cgacgaaatg tcaatttacc atcacattat gacgatatgt
     1021 ttattttaaa cacacaagct catgcgcgtc ttgatcaaat ggcacaacag tttgaagttg
     1081 tttgtaatgg cttgaacgaa aatgaaggac aagcaattca aacgatggat caatctgcct
     1141 ctctaatacg gtcaaactta attcaagtta aagaacaatt agaaaaacta gctgtatact
     1201 aattaattta ttaaatgcta cttgttttca ttgagaataa gtagcttttt caaacataaa
     1261 agttttacaa acacataaat gggtgatgag ctatgtttaa aagaactaaa ctaatcttaa
     1321 tagcaacatt actgctatca ggatgttcaa ctaccaataa cgaatctaat aaagaaacaa
     1381 aatcagtgcc agaagaaatg gaagcttcaa aatatgtagg tcaaggcttc caaccacctg
     1441 cagaaaaaga tgtggttgaa tttgcgaaaa agcataaaga taaaattgcc aaacgaggcg
     1501 aacaattttt tatggataat ttcggtctaa aagttaaagc taccaatgtt gtaggtagtg
     1561 gaaaaggcgt agaagtattc gtgcattgtg atgaccacga tatcgtattt aatgcgagta
     1621 ttccatttga taaatcaata attgagagtg atagctcatt aagaagtgag gacaaaggcg
     1681 atgatatgag tactttagtt ggtacagtgt tgagtggctt tgaatatcga acacaaaaag
     1741 aaaagtatga caatttatat aaatttttca aagataatga agagaaatac caatatacag
     1801 gctttacaaa agaagcaata aacaagacgc aaaatgtcgg atataaaaat gaatattttt
     1861 atatcactta ctcgtcaaga agcttaaaag aatatcgtaa gtattacgaa ccattgattc
     1921 ataaaaatga taaagaattt aaagaaggaa tggaacaagc cagaaaagaa gtgaattacg
     1981 ctgctaatac agatacagta acaacattgt ttagtacaaa ggaaaatttt actaaagaca
     2041 atacagttga tgatgtaatt gaactgagtg ataaactata taattttaaa aataagccag
     2101 aaaaatctac aattacaatt caaataggaa agcctactat taatactaaa aaagcctttt
     2161 atgatgataa tgatccaata gaatatggag tgtatcgtaa agatgaataa atttttttaa
     2221 aatgcttttt aatattctat ataaacacat aaatgggtga tgagctatgt ttaaaaaggc
     2281 gaaattaatc ttaatagcaa cgctactgtt atcaggatgt tcagcgatga ataatgaatc
     2341 aaaaaaagac acaaatacag aaacaaatac agaaacaaaa tcagtaccag aagaaatgga
     2401 agcttcaaaa tatgtaggac aaggctttca accacctgca gaaaaagatg cgattgaatt
     2461 tgtgaagaag catcgtaaag aatttgaaaa agtaggtgaa caattcttta aagataactt
     2521 tggactaaaa gttaaagcta caaatgttgt aggtaaagat gatggtgtag aagtttatgt
     2581 gcattgcgaa gatcatggca ttgtatttaa tgcaagtcta cctttgtaca aagatgccat
     2641 ccatcaaaaa ggatcaatgc gtagtaatga caacggtgat gatatgagta tgatggtggg
     2701 tacagtgctg agtggctttg aatatcgagc gcaaaaagaa aagtatgata atttatataa
     2761 atttttaaaa gaaaatgaaa agcaatatca atatacaggt tttactaaag aagcaattaa
     2821 caagacgcaa aacgtcgggt atcaaaatga atatttttat attacatatt tatcaagaaa
     2881 tttaaaagaa tatagaaaat attacgaacc attgatacat aaaaatgata aagagtttaa
     2941 agaaggtatg caacgagcta gaaaagagct aaactatact gctaatacaa atactgtagc
     3001 aacgttgttt agtacgaatg atgaaaggaa tagaaaagaa aagataaata atgtaataga
     3061 tttatccgag aaaattgaaa gaacaaaaga tatgccaatc aagaatacta taactactca
     3121 attaggaaat aaacttattg gcacaaaaaa agctcgtttt gatgataaga aagtagtgtc
     3181 gtttggagca tttgaagatg aataaaataa atgatagaga tttaacagaa ttgagtagct
     3241 atagggttta tcaagacatc aataaagata atgactttac agttaacgaa aaacgattta
     3301 agcaggcaga tgtatttgaa gatttatata gagagaaact aaaagacaca aataaattaa
     3361 gagagtataa ttatttacaa aatgaaactt ttaaaagcgc ataaataggt gatgagatat
     3421 gcttaaaaaa gcaaaattta tcttaatggc aacgatacta ctatcaggat gttcaactac
     3481 caataacgaa tccaacaaag aaacaaaatc tgtaccagaa gaaatggatg cttcaaaata
     3541 tgtaggacaa ggattccaac cacctgcaga aaaagatgcg attgaatttg caaagaagca
     3601 taaagataaa attgctaagc gaggcgaaca attttttatg gataacttcg gtctaaaagt
     3661 taaagctaca aatgttatag gtagtggcga tggtgtagaa gtattcgtgc attgtgatga
     3721 ccacgacatc gtatttaatg cgagtattcc atttgataaa tcaattattg acagtgatag
     3781 ctcattaaga agtaaggata aaggtgatga tatgagtact ttagttggtg cagtactcag
     3841 tgggtttgaa tatcgagcac aaaaagaaaa atatgataaa ttatataaat ttttcaaaga
     3901 taatgaagag aaatatcaat atacaggatt tacaaaagaa gcaattaata agacgcaaaa
     3961 tagtggttat gaaaatgaat atttttatat ttcggccata ccttataatt tagctgagta
     4021 tagagactat tttgaaccat tgttaaacaa aagtgacagt gaattttcaa aagaattgtc
     4081 aaatgttaag aagcaattaa aagataagtc taaagtttcg gtaactacta ctctatttag
     4141 taaaaaaaag aactatacta aaaaaagtaa cagtgaaaat gtaataaaaa tggcagaaga
     4201 aataaaaaaa gataaagaga taccaaacgg tatagagctt agtataaaat tttcggacaa
     4261 taaaataaat acggttaaac caaattttaa cggtgaaagc acttcagaat atggtgtgtt
     4321 tgatcaagaa taaaattaat gatgaaaatt taacggagaa tagtgtatat tgagtagatc
     4381 aagaataaaa agataattct actattgttg tgaaggcaaa taagtagaag attttaagtg
     4441 taatttctgg tgatttaaat aataatataa atggaagtac tgatataaaa ctttttaacc
     4501 tactagattc ttataatttg ctttccattt tatgacgatt tttactcaat tgagtgatag
     4561 aatcaaaaaa gccatctcaa aaattaatca agcaaacaac attccaaaca atgctcgcaa
     4621 atcaccaatg tatcactctc caattacgta actatgattt aatttaagca tagtta
//
LOCUS       DAKTLH010000169         4575 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.173, whole genome shotgun sequence.
ACCESSION   DAKTLH010000169 DAKTLH010000000
VERSION     DAKTLH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.173"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..517)
                     /locus_tag="O0394_002447"
     CDS             complement(<1..517)
                     /locus_tag="O0394_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCY0500503.1"
                     /translation="MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITS"
     gene            complement(489..1463)
                     /locus_tag="O0394_002448"
     CDS             complement(489..1463)
                     /locus_tag="O0394_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HCY0500504.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMHKYI"
     gene            complement(1515..2138)
                     /locus_tag="O0394_002449"
     CDS             complement(1515..2138)
                     /locus_tag="O0394_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0500505.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            complement(2135..2464)
                     /locus_tag="O0394_002450"
     CDS             complement(2135..2464)
                     /locus_tag="O0394_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HCY0500506.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            complement(2464..3450)
                     /locus_tag="O0394_002451"
     CDS             complement(2464..3450)
                     /locus_tag="O0394_002451"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCY0500507.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            complement(3447..3650)
                     /locus_tag="O0394_002452"
     CDS             complement(3447..3650)
                     /locus_tag="O0394_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCY0500508.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            complement(3631..>4575)
                     /locus_tag="O0394_002453"
     CDS             complement(3631..>4575)
                     /locus_tag="O0394_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCY0500509.1"
                     /translation="MILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEH
                     ILMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGL
                     IGSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIG
                     YYYKVNRNIFWILLIGMLVIFIALQIRIFTIKEDNIYNKLIKDDMTSGNYDNAQNIVK
                     QTINKNYADDQTYYLSGMIMATINSKSEGMTEWERGLRMFPKSGLLNFELAIANRSLN
                     DDEKALKYVRKALNADPKNTDYINLEKELTKSNESKNK"
BASE COUNT         1522 a          833 c          591 g         1629 t
ORIGIN      
        1 agctggtaat gaaaaaagac aatgtttttt gaaaagaaga tagttgaata ttcatagaag
       61 tatataattg ttgaaactgt ggaattactg tgaggtttaa tataataatc atagcaataa
      121 agatagaaac tagtattaag gggtattgca gtgtctttaa caatcgttgt tctgactttc
      181 tattcacttt catataattt acggtttctt ctagaacgtc tataatattg ccaaatcttt
      241 ctgccaaata tacttgcatg acgatagtat cgctataacc tatcagtgat aatatttgat
      301 tgcatggtgc accatttgaa atttcactta gaatggtggt acctaattgc ttatttttat
      361 atgtcatttg aagatttaaa aattgaaaac tttgatacag agtgaaacca tatttcaaca
      421 aattacataa atttgaaagt aagtcgattt gttgggcctt acttaattgt cgcttcttag
      481 aatgtagttt aaatgtattt atgcattgta gtttcacaaa tgacacctgc ttttgtcata
      541 tcatcaagtt tatcttctaa gttcttaaat gatgatggta atgaatgatt atgggaaaag
      601 aaatatcgga gttgttgctg agatagaatt tcacatacta attgtcgctg ttgcttaata
      661 gtagttacaa gtcgttggtt tataattaag ttagttgcct gtatcaattc ttgtacagaa
      721 atgcccattt ctaatagcct taaaatagca cctttacaat cagttgcatg caatgtagtc
      781 agaacaaggt gaccacttaa actagcctgt ataacacact tggcaacatc tttatctctg
      841 atttcaccta ttaaaataac atcaggatca catcttaaaa tagctttaaa cgaatttaca
      901 tagtttatgc cagctttatc attcacatta atttggacga taccaggaat ttgcatctct
      961 acaggatcct ctatagaaat tacatttaaa ttcaaggctt tattcgcgta tgagaccatt
     1021 tgatacatta atgtactctt tcctgaacca gttggtccac taaacaatag taatccttgt
     1081 ttcttattca tgaggtgttt aaaatcattg aatttataag ttgatttctg tggttgaaaa
     1141 aattgaggta caattctgat aacacaactt tcttggccaa gtgacaatgg taaagttgat
     1201 attctcaaaa aatatatttt attgaaaagg taactatatc gaccgctctg tgcgacttgc
     1261 tgtgtagaaa catcaagccc agcttgaaac ttcatataaa ctaataactt ttgataaatg
     1321 ctattcccaa tttgttcata ctgctccaag ttatcattaa ttctaaattt aatacttact
     1381 tcatttttaa ctggaataaa atgtacatca ctcgctttca tttctatcgc tttattaatt
     1441 atttcttgaa atagaatctt caaaaaaaca cctcctacat ataatcacgt aggaggtgtt
     1501 tttattactt caatttaacc gtgtaaaaat ggatttaatt gttcatcatc aaccgtcgta
     1561 tatggaccat gtccagggaa taaaggtaaa tcgccttcta attcaaatat tttatcttga
     1621 atagaatcaa ctagcgtttc ataatcacct ttatataaat ctgtacgtcc gattccatta
     1681 ttaaataatg tatctccaac aactgcgaat tcatcgaaca catatgttaa acttcctggt
     1741 gaatgtccag gtgtgtgtaa cacattaaac ttaaatcctt ctatttctgt gctaccttcg
     1801 tttaactttt caggagttac cttacttgta ataattggta atccatattg cttaaattta
     1861 tctgccccat ttttaacggg atcttttaga aaatcaaact ctgcttcatg catataaacc
     1921 gggacatcga atcgatcaac tatatcatcg actgctccga tatgatcaaa gtgtgcatgt
     1981 gttaataaaa tagcttttaa cggtttattt atttggttta attttttaat aattttttca
     2041 ctttcacctg aagggtcaat cagaataaca gctttgtcat tttcgatgaa atacgtatta
     2101 gtatcaacta agcctaaagt taagcttgaa atcctcatag gttttcacca ctattttcta
     2161 aatgtttttg tactgatgtt agtttatcat tcatttttct agatttgtct cgtcggtcat
     2221 caatacggat atttgtacaa actctactta aacctttatc aaaaggtaat tcatgtatca
     2281 cttgcacaac ttctaataca tcgcttaatt caccttcaat tagagtattc attggtgtta
     2341 attgaaaatc aattttaccc attgctttat attcttgaag ttttttctga atatctgcaa
     2401 tatatttact aacactcgga ccttccgttc caactggaat aacaaccaca tcaacaatag
     2461 ccattatttt accccctctt tatctaatac ataggtcttg attaatcctg ctgctcctgt
     2521 aatacctgca tcattaccta atttcgcttg tacaatttca gtttcaaatt gagcaggtgc
     2581 aaatgttaaa ttatgatatt ctgttttaat attttcaatt aaaataggtc ctgcagtaga
     2641 cattcctcca cctagaacga tatatttcgg attacttgta acactaataa tactacataa
     2701 atatccaata tagtttgcaa ccttttcagt aatgaaaata cagaattggt caccagcttt
     2761 tgccgcatca aaaacagctt ttgctgtaac cttattttct ttaatcaatt ctaatataga
     2821 agatctaaac gtcaacttcg gatagtagaa gttaactaag ttaacaacgc ctgtcgctga
     2881 agcaactgtt tcaatacatc cagaacgacc acaattacat ttaaatcgtt gatcgaagtc
     2941 tgctctaaaa tgacctattt ctgcgccaga gccattatga ccatgtacga tttcaccatt
     3001 tgaaataatt cctccaccta gacctgtacc aagtgtgatg gcaacaacat catcggcacc
     3061 ttcaccagca cctttgtgtt tctcccctaa agcagctatg ttagcatcat tatctacata
     3121 cactggacaa tcaacgaatt gttcaaaaat ctcacgtaca ttaacttttt ctggccaata
     3181 taagtttact gctccattta ctgtaccttt ttcaaagtca acaggacctg gtacaccaat
     3241 acctacgcca agtacatttg aaaaattata attattttca tttacttttt caacaaacga
     3301 atcataaatt cctttcaaaa gtgtatatcc tgtactatca gatgtatcag tgtgaataga
     3361 ccatttatgt aattgttcta attcaggtgt gaaaatacct aatttacaag tcgtcccgcc
     3421 tacatcagct gctaaaataa tttttgtcat ttctgttcat tccttctctg attaattatc
     3481 aacgtacatt tcaaatattc ttccttagat aacagttcat attgatacaa tgatgaaatc
     3541 tcctgttgaa tcatttcgta catatcttct ggatttttaa aatatattag aaatccgtaa
     3601 cttttcaata actgttgtac atcataaaag ttatttattt ttcgactcat ttgatttagt
     3661 caactctttt tctaagttaa tataatctgt atttttaggg tctgcattta atgctttacg
     3721 cacatatttt aatgcttttt catcatcatt taatgaacga tttgctatcg ctaactcaaa
     3781 atttaataaa cctgatttag gaaacattct aagtcctcgc tcccattctg tcataccttc
     3841 ggacttagaa ttgatagtag ccataatcat accacttaaa taatatgttt gatcatcggc
     3901 ataattttta tttattgtct gcttcacaat attttgagca ttatcataat taccactagt
     3961 catatcatct ttgatcaatt tattataaat attatcttct ttaattgtaa aaattctaat
     4021 ttgaagtgca ataaatataa caagcatacc aattagtaaa atccaaaaaa tattacgatt
     4081 cactttatag taatagccaa ttaaagttat taataaacca ccaatgaatc ctccaatatg
     4141 cgccacaata tttatatttg acataaacag agaaacacca actaatatca ctaatgcaat
     4201 taataactgt cctaacattt ttttgttaaa tgtttttgaa acatacatca tcgcaaaaat
     4261 tgatccaatc agaccaaata tagcaccact agccccaact gaaattgtag tcgtattaaa
     4321 tgatagtgat acaaagtttc caaacaaccc tgcaataaag tatacagtta acatccgcca
     4381 tgaaccaata attgcttcga ctattttacc aaaaataaat aatgaaagca tattcataag
     4441 tatatgttca aaactaaaat gtaaaaacat cgatgtaaca attcgatacc attcaccatg
     4501 tacgacatta aaatgcacta acccgccaac atctaataat tttacatccg aaaaattatt
     4561 taaatataaa atcat
//
LOCUS       DAKTLH010000170         4550 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.174, whole genome shotgun sequence.
ACCESSION   DAKTLH010000170 DAKTLH010000000
VERSION     DAKTLH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.174"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            321..3143
                     /gene="fnbB"
                     /locus_tag="O0394_002454"
     CDS             321..3143
                     /gene="fnbB"
                     /locus_tag="O0394_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbB"
                     /protein_id="HCY0500510.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTT
                     VEESGSSATESKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPKTVQAP
                     KVETSRVDLPSEKVADKETTGTQVDIAQPSNVSEIKPRMKRSTDVTAVAEKEVVEETK
                     ATGTDVTNKVEVEEGSEIVGHKQDTNVVNPHNAERVTLKYKWKFGEGIKAGDYFDFTL
                     SDNVETHGISTLRKVPEIKSTDGQVMATGEIIGERKVRYTFKEYVQEKKDLTAELSLN
                     LFIDPTTVTQKGNQNVEVKLGETTVSKIFNIQYLGGVRDNWGVTANGRIDTLNKVDGK
                     FSHFAYMKPNNQSLSSVTVTGQVTKGNKPGVNNPTVKVYKHIGSDDLAESVYAKLDDV
                     SKFEDVTDNMSLDFDTNGGYSLNFNNLDQSKNYVIKYEGYYDSNASNLEFQTHLFGYY
                     NYYYTSNLTWKNGVAFYSNNAQGDGKDKLKEPIIEHSTPIELEFKSEPPVEKHELTGT
                     IEESNDSKPIDFEYHTAVEGAEGHAEGTIETEEDSIHVDFEESTHENSKHHADVVEYE
                     EDTNPGGGQVTTESNLVEFDEDSTKGIVTGAVSDHTTIEDTKEYTTESNLIELVDELP
                     EEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELGYEGGQ
                     NSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNNGNQSFEEDTEKDKPKYEQ
                     GGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTLPQVSGHN
                     EGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPTEPGK
                     PIPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVVPTKKAQSKKSELPETGGEESTN
                     NGMLFGGLFSILGLALLRRNKKNHKA"
     gene            3323..4189
                     /gene="galU"
                     /locus_tag="O0394_002455"
     CDS             3323..4189
                     /gene="galU"
                     /locus_tag="O0394_002455"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCY0500511.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKALGL"
     gene            complement(4400..>4550)
                     /locus_tag="O0394_002456"
     CDS             complement(4400..>4550)
                     /locus_tag="O0394_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0500512.1"
                     /translation="LNNKGMLIKERSHADERIVLIKINKIQYNTIKSIFTDTSKILKP
                     RKFFF"
BASE COUNT         1773 a          662 c          891 g         1224 t
ORIGIN      
        1 agtgacttgt taatgatgca ttgtacaggc gattattatg tatggtttag tatattgttt
       61 taaattagat gaatttgaat ttaatttaaa aagtaagtat aaaaaataca agcctgttat
      121 atcaaggtgt tcaagagctt tgtatgcaat atatatgtga gtttcaaata ataaaaaact
      181 cattatacat aaagttaaca acaatcttaa ctttttatta actcgctttt tttcattgct
      241 tttaaaaacc gaacaatata gaattgcatt tattgagttt taaaaataaa tgaattttgc
      301 atttaaggga gaatattata gtgaaaagca atcttagata cggcataaga aaacacaaat
      361 tgggagcggc ctcagtattc ttaggaacaa tgatcgttgt tggaatggga caagaaaaag
      421 aagctgcagc atcggaacaa aacaatacta cagtagagga aagtgggagt tcagctactg
      481 aaagtaaagc aagcgaaaca caaacaacta caaataacgt taatacaata gatgaaacac
      541 aatcatacag cgcgacatca actgagcaac catcacaatc aacacaagta acaacagaag
      601 aagcaccgaa aactgtgcaa gcaccaaaag tagaaacttc gcgagttgat ttgccatcgg
      661 aaaaagttgc tgataaggaa actacaggaa ctcaagttga catagctcaa ccaagtaacg
      721 tctcagaaat taaaccaaga atgaaaagat caactgacgt tacagcagtt gcagagaaag
      781 aagtagtgga agaaactaaa gcgacaggta cagatgtaac aaataaagtg gaagtagaag
      841 aaggtagtga aattgtagga cataaacaag atacgaatgt tgtaaatcct cataacgcag
      901 aaagagtaac cttgaaatat aaatggaaat ttggagaagg aattaaggcg ggagattatt
      961 ttgatttcac attaagcgat aatgttgaaa ctcatggtat ctcaacactg cgtaaagttc
     1021 cggagataaa aagtacagat ggtcaagtta tggcgacagg agaaataatt ggagaaagaa
     1081 aagttagata tacgtttaaa gaatatgtac aagaaaagaa agatttaact gctgaattat
     1141 ctttaaatct atttattgat cctacaacag tgacgcaaaa aggtaaccaa aatgttgaag
     1201 ttaaattggg tgagactacg gttagcaaaa tatttaatat tcaatattta ggtggagtta
     1261 gagataattg gggagtaaca gctaatggtc gaattgatac tttaaataaa gtagatggga
     1321 aatttagtca ttttgcgtac atgaaaccta acaaccagtc gttaagctct gtgacagtaa
     1381 ctggtcaagt aactaaagga aataaaccag gggttaataa tccaacagtt aaggtatata
     1441 aacacattgg ttcagacgat ttagctgaaa gcgtatatgc aaagcttgat gatgtcagca
     1501 aatttgaaga tgtgactgat aatatgagtt tagattttga tactaatggt ggttattctt
     1561 taaactttaa taatttagac caaagtaaaa attatgtaat aaaatatgaa gggtattatg
     1621 attcaaatgc tagcaactta gaatttcaaa cacacctttt tggatattat aactattatt
     1681 atacaagtaa tttaacttgg aaaaatggcg ttgcatttta ctctaataac gctcaaggcg
     1741 acggcaaaga taaactaaag gaacctatta tagaacatag tactcctatc gaacttgaat
     1801 ttaaatcaga gccgccagtg gagaagcatg aattgactgg tacaatcgaa gaaagtaatg
     1861 attctaagcc aattgatttt gaatatcata cagctgttga aggtgcagaa ggtcatgcag
     1921 aaggtaccat tgaaactgaa gaagattcta ttcatgtaga ctttgaagaa tcgacacatg
     1981 aaaattcaaa acatcatgct gatgttgttg aatatgaaga agatacaaac ccaggtggtg
     2041 gtcaggttac tactgagtct aacctagttg aatttgacga agattctaca aaaggtattg
     2101 taactggtgc tgttagcgat catacaacaa ttgaagatac gaaagaatat acgactgaaa
     2161 gtaatctgat tgaactagta gatgaactac ctgaagaaca tggtcaagcg caaggaccaa
     2221 tcgaggaaat tactgaaaac aatcatcata tttctcattc tggtttagga actgaaaatg
     2281 gtcacggtaa ttatggcgtg attgaagaaa tcgaagaaaa tagccacgtg gatattaaga
     2341 gtgaattagg ttacgaaggt ggccaaaata gcggtaatca gtcatttgag gaagacacag
     2401 aagaagataa accgaaatat gaacaaggtg gcaatatcgt agatatcgat ttcgatagtg
     2461 tacctcaaat tcatggtcaa aataatggta accaatcatt cgaagaagat acagagaaag
     2521 acaaacctaa gtatgaacaa ggtggtaata tcattgatat cgacttcgac agtgtgccac
     2581 atattcacgg attcaataag cacactgaaa ttattgaaga agatacaaat aaagataaac
     2641 caaattatca attcggtgga cacaatagtg ttgactttga agaagataca cttccacaag
     2701 taagtggtca taatgaaggt caacaaacga ttgaagaaga tacaacacct ccaatcgtgc
     2761 caccaacgcc accgacacca gaagtaccaa gcgagccgga aacaccaaca ccaccgacac
     2821 cagaagtacc aagcgagccg gaaacaccaa caccgccaac gccagaggta ccaactgaac
     2881 ctggtaaacc aataccacct gctaaagaag aacctaaaaa accttctaaa ccagtggaac
     2941 aaggtaaagt agtaacacct gttattgaaa tcaatgaaaa ggttaaagca gtggtaccaa
     3001 ctaaaaaagc acaatctaag aaatctgaac tacctgaaac aggtggagaa gaatcaacaa
     3061 acaacggcat gttgttcggc ggattattta gcattttagg tttagcgtta ttacgcagaa
     3121 ataaaaagaa tcacaaagca taatcaatcc aaaattgaca ggtttatttc ataaattata
     3181 tgaagtaagc ctgtttttta aaattaaaac aaatttccca agaaataatt acatactcaa
     3241 tgacactatg aaggcgttct aattagtgtt aaaatgacgt tgatacatag atttaatact
     3301 taggaaaagg agcacattaa ctttgaaaaa aataaaaaag gcaatcattc ccgctgctgg
     3361 tttagggact agatttttac cagcaactaa agcgatgcca aaggaaatgc ttcctatctt
     3421 agataaaccc acaatacaat atatcgttga agaagctgca agagctggaa ttgaagatat
     3481 tattatagtg acaggtcgcc acaaacgcgc gattgaagat cattttgata gtcaaaaaga
     3541 attagaaatg gtgttaaaag aaaaaggtaa atctgaatta ctagagaaag ttcagtattc
     3601 aacggaactt gcgaatattt tttatgtaag gcagaaagaa caaaaaggtt tagggcatgc
     3661 gattagttct gcgcgtcaat ttatcggcaa tgaaccattt gcggtattat taggtgacga
     3721 tattgttgaa tctgaagtgc cagcagtgaa acagttgatt gacgtttatg aagaaacagg
     3781 gcactcagtt ataggtgtcc aagaagttcc ggaagcagat actcatcgtt atggaattat
     3841 tgacccatta actaaaaacg gacgtcagta tgaagtgaaa aagtttgttg aaaagccagc
     3901 acaaggtacg gcaccatcaa acctagctat tatgggacgt tatgtattaa cgcctgaaat
     3961 tttcgattat ttaaaaacgc aaaaagaagg cgcaggtaac gaaattcagt taacagatgc
     4021 gattgaacgt atgaataatg ataaccaagt gtatgcatat gacttcgaag gcgaacgtta
     4081 tgacgttgga gaaaaattag gcttcgttaa aactaccata gaatatgcat tgaaagatga
     4141 cagtatgcga gaagaattaa cacgatttat taaagcatta ggtttataac attaaaagca
     4201 tgatactcag taaaattgga acacgaaatg gtgaactaaa ttgaatgagt gtcatgctta
     4261 atttatgtgt tgtgatatat agtagggtat tgaaataata taaaaacaaa agaaagttaa
     4321 ttatgtattt ttatttatac taaattgaga agtagtatga ttgcttattt attttatcaa
     4381 gaggtaaatc tgtttaaatt taaaagaaaa attttcttgg tttgagaatc ttggaagtat
     4441 ctgtgaatat gcttttaata gtgttatatt gtattttatt tattttgatt aacacaatcc
     4501 tttcatctgc atgggatcgt tctttgatta gcataccttt attatttaat
//
LOCUS       DAKTLH010000171         4520 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.175, whole genome shotgun sequence.
ACCESSION   DAKTLH010000171 DAKTLH010000000
VERSION     DAKTLH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.175"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..299)
                     /locus_tag="O0394_002457"
     CDS             complement(<1..299)
                     /locus_tag="O0394_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002434158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500513.1"
                     /translation="MENTNSNMHKNDNSYVLSALSYLSIFFAPVILPLFVWILADEPT
                     SEHGKKAFINHIMTWVSFFIGRLAFIFSKEVFDKPLDHQLLIFSILLIITIIFFL"
     gene            complement(829..1679)
                     /locus_tag="O0394_002458"
                     /pseudo
     CDS             complement(829..1679)
                     /locus_tag="O0394_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2192..2689)
                     /locus_tag="O0394_002459"
     CDS             complement(2192..2689)
                     /locus_tag="O0394_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0500514.1"
                     /translation="MKLRALEYSDLLFVHELNNEYSIMSYWFEEPYESLTELQHLFDK
                     HLLDESERRFIVEDENQVVGIVELVEINYIHRNCEIQIIIKPEFSGKGYAKFAFEKAI
                     IYAFNILNMHKIYLYVDADNKKAIHIYESQGFKTEGLLKEQFYTKGKYKDAYFMSLLK
                     SEYIL"
     gene            3187..4314
                     /locus_tag="O0394_002460"
     CDS             3187..4314
                     /locus_tag="O0394_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HCY0500515.1"
                     /translation="MKTRAAVLHEMGAKKPYKESKPLKIEYLELDNPSEHEVLIKIHA
                     AGLCHSDLSVINGNRPRPLPMALGHEASGEVIKVGKAVTRVSEGDHVVCTFIPSCGKC
                     IPCKEGRPALCENGAISNEKGEMLEGGMRLSNDEGKVYHHLGISGFAEYSVVSENSIV
                     KIDKKIPFERAAAFGCAIITGIGAVVNTAQIRSGSNVAVVGLGGIGLNAIIGAKLAGA
                     NEIIALDINEDKFELAKQFGATATFNSSDKDIDEQIKEYIPGGVEYAFETAGVVPAMK
                     VAYQITKRGGTTVTTGLPNPKDNFSFPQVTLAAEERTIKGSYVGSCVPDRDIPRFVNL
                     YNQGRLNIDSLISEVITLDEINEGFDRLSNGEVGRIIMKMH"
BASE COUNT         1584 a          645 c          685 g         1606 t
ORIGIN      
        1 attaaaaaga atataatagt aataattaaa agaatgctaa atattaataa ttgatgatcc
       61 aaaggtttat caaagacttc tttagaaaaa ataaatgcca atctacctat aaaaaaacta
      121 acccaagtca taatatgatt aatgaatgct tttttgccat gttctgatgt tggttcatca
      181 gcaagtatcc atacaaataa aggtaaaatg acaggtgcga aaaagatact taaataactc
      241 aatgctgata aaacgtaact attgtcattt ttatgcatat tactatttgt attttccact
      301 tttttcacct tctttacttt tctttactat tataaaaaca ttaatagcga gctattacaa
      361 cagttataag agaagcttac gatattgtaa gtatgtgttg ttataatcaa tataaaatta
      421 agaaagacct gagaatttat attatcatcc acaatcgaag tgagtagcga aatttattgg
      481 aaaaggtact tatattgaag atattgttaa tggttcattt ttaaaaagcc aagacgattt
      541 gaagttaaaa gcttctgact tcactgtcga agataactat aactacggtg tacttatatt
      601 tccagagaat ctttctctta cagataataa taagaaattg gaatctttaa taaaaactat
      661 actatttaca ggtaaacgat atcaatatac ttattttaaa atttgatctt aatagataag
      721 gatataatga tttaaacaat ttaagagata ttattttcac aaaaaaagaa tgctttttgt
      781 tagaggcact gaacccttaa aatgggaact taataaaaac actatgttct aggctgctaa
      841 ttctctgtat tttacagggg ataagtagcc tagtttttgt tgaattcgat tattattata
      901 gtttttaatg tattttcgac aatatctatt acaatatgat tagagttatt aagctgatta
      961 ttgatgtaaa aagtttcaga ctttagcgag gaatggaaac tttctatcgg ggcgttatcg
     1021 gcaggtgttc cctttcggga catacttctg ataatgcctt tttcttcgca taattgataa
     1081 taagcataag atgtataaac gctgccttga tcactatgta atatacaccc ctcaggtata
     1141 tcgatttgat ttaatgtatc attaactaaa cgttggtctt gtttatcatc tattttatac
     1201 gccacaattt ctccgttata aatgtccatt atcgaagata aatacaacat agaatgacca
     1261 aatggtaaat aagtaatatc ggttgttaat acttccatgg gacaactcgc tttaaattgt
     1321 ctttgtaata aattgtccgt tttataatac ggtttaccta ttcttgtcgt ctttttaggt
     1381 ctaactcggc agttcaaatg atgcttctgc atcactctct gtactctctt atgattaatt
     1441 ggtgatgtat aacattgatt aatcaatgct gtaatcttac gataaccgta ggtatattgt
     1501 ttaccaacat gttgtgaaaa tctataactt tccccatttc gataccatcg ccaccaattt
     1561 ttcacttgtg ttctatttct aatatttaat tctttcataa tttcttttgt tggaaatcct
     1621 gctgctttca tttcaactgc tttatacttt gtttctactg aataagaagc tcttttcata
     1681 gaaaaaaaac acctccgtat gattcatttt aatatgaatt caacgaaagt gtttttatat
     1741 aattcccact attggggtca gtctaaaatt aaaattgata acttttttaa taaaatagat
     1801 acactaattt taaaacaagg taaaaaaata gaattattaa aacaacgcaa acaaggctta
     1861 ctacaaaaaa tgtttgtgta attctgataa cgtcctatta tgttaattga aaatagacaa
     1921 aaataaattg agagcatccc ttaccgcaaa gtgaaggatg ctctattttt gttgaaatta
     1981 atataagctt cattctgata gatagtcatt aataattgtt tgcaaatcgt tattaagtac
     2041 cgttgttaca gcgtcatcat tcataaaatc ctcgtagatt ttatcaagaa tttattcctc
     2101 ttacagacat tcgcgagaag tccgttttaa ttttattaga agtaattcag gtttgaacct
     2161 acctaaatga atatatgagt tatcttttat actacaaaat atattcagat ttcaataatg
     2221 acataaaata ggcatcttta tatttacctt tagtgtagaa ttgctcttta agtaatcctt
     2281 ctgttttaaa tccttgtgac tcgtatatat gaatagcttt tttgttatct gcatcaacat
     2341 atagatagat cttgtgcata tttaaaatat tgaatgcata aattatcgct ttttcgaatg
     2401 cgaattttgc ataaccttta ccactgaact caggtttaat aattatttgt atttcacaat
     2461 tacgatggat gtaattaatt tctactaatt caacaatacc tacgacttga ttttcatctt
     2521 caacaataaa acgtctttcc gattcatcta ataaatgctt atcaaataaa tgttgaagtt
     2581 ccgttaagga ttcataaggc tcttcaaacc aataagacat aatagaatat tcattattta
     2641 attcatgaac aaaaagtaaa tcactatact ctaatgctct tagtttcata atcccactcc
     2701 caaaattttc ttatatattt gcattataaa tataaataac gaataagtca ttattcacta
     2761 tgaataaatc tattttaaca attcactaca tactaattct cattttctta ttattctcta
     2821 atatctctga tttattactc agtgaaagat gccctatttt tatcagtata tagtttttat
     2881 tttgatagat attgattaat aatcgtctgt aaatcattgt taagtaaggt tgtgatagcg
     2941 tcgttattca taaaatcttc ataaatttta tcaagtattt ctttaatata ttttttaatc
     3001 ttcacacctt aaattcactt gaaaaccaaa tcacttctaa attatgattt tacataataa
     3061 atttttaaaa attggtatta tataaaataa tctcaataat cagaattttt tgtaaataat
     3121 aatattaaag tgtaaaataa cagataagaa tagacttaat gtatataaat taaaggagtg
     3181 ttggaagtga aaactagagc agcagtttta catgaaatgg gggctaaaaa gccctataaa
     3241 gaatctaaac ctttaaaaat tgaatactta gaacttgata atcctagcga acacgaagta
     3301 ttaattaaaa ttcatgcagc tggattatgt cactctgatt tgtcagtaat taatggtaac
     3361 agacctagac ctttacctat ggcacttggt catgaagctt ccggtgaagt aattaaagtt
     3421 ggaaaagctg ttacaagagt tagcgaagga gatcatgtgg tatgcacatt tattcccagt
     3481 tgtggaaaat gtatcccatg taaagaagga cgtcctgcat tatgtgaaaa cggagcaata
     3541 tctaatgaaa aaggcgaaat gttagaggga gggatgcgtt tatctaatga tgaaggaaaa
     3601 gtatatcatc acttggggat atcaggtttt gctgaatatt ctgttgtctc tgaaaactct
     3661 atagttaaaa ttgataaaaa aatacctttt gaacgtgcag ctgcatttgg ttgtgctatt
     3721 atcacaggta tcggtgctgt ggtgaataca gcccaaattc gttctggtag taatgtagcg
     3781 gttgttggtt taggaggtat tggattgaat gctattattg gagctaaact agcgggagcc
     3841 aatgaaatta ttgctttaga tattaacgaa gataaatttg aattagcaaa gcaatttggg
     3901 gctacagcaa catttaattc aagcgataaa gatatcgatg agcaaattaa agaatatatt
     3961 cctggcggag tagaatatgc ttttgaaaca gcgggtgtag tgccagctat gaaagttgct
     4021 tatcagatta ctaaacgagg ggggacaact gtaacaacag gactgcctaa tcctaaagat
     4081 aatttttctt tccctcaagt tactttagcg gctgaagaac gtaccattaa aggatcatat
     4141 gtaggaagtt gtgtacctga cagagatata ccaaggttcg ttaatctata caaccaagga
     4201 cgtttaaata ttgattcact catcagtgag gttatcactt tagatgaaat taatgaggga
     4261 tttgatcgtt tgtctaatgg tgaagtaggt agaattataa tgaagatgca ttgaatataa
     4321 tagaattcaa gtcgttcctc ctcttgattt ctatgaaaag aataacaatt tgagtaggaa
     4381 gattactgac aaatattaca tttgcattgt gactgccaat ctaattgtat ttttacattt
     4441 catatctata tattagaaga taaatgtttt aaaactaatt actaatagtt tttttagata
     4501 aaaaaagata taggagcatc
//
LOCUS       DAKTLH010000172         4486 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.176, whole genome shotgun sequence.
ACCESSION   DAKTLH010000172 DAKTLH010000000
VERSION     DAKTLH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.176"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            1..935
                     /locus_tag="O0394_002461"
                     /pseudo
     CDS             1..935
                     /locus_tag="O0394_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(998..1831)
                     /locus_tag="O0394_002462"
     CDS             complement(998..1831)
                     /locus_tag="O0394_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0500516.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNNVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQRIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHAVVTIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKKFE
                     G"
     gene            complement(2043..2951)
                     /locus_tag="O0394_002463"
     CDS             complement(2043..2951)
                     /locus_tag="O0394_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HCY0500517.1"
                     /translation="MDLSSPIVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNLKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            complement(3076..4269)
                     /locus_tag="O0394_002464"
     CDS             complement(3076..4269)
                     /locus_tag="O0394_002464"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCY0500518.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     regulatory      complement(4375..4485)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         1447 a          826 c          650 g         1563 t
ORIGIN      
        1 attaagaagt tcaaaaagaa atttgatgat tattccgaaa gaaaaaataa atatgaagaa
       61 caaaaatcga ttcttaaaga tagaaatagt ttttctaaaa ctgatcatga tgcaactttt
      121 atgagaatga aggaagacca tatgaaaaat ggccaactta agccaggata caatttacaa
      181 atagcgacaa attctcaatt tgttttatcc tatgacctat ttcaaaaccc gacagatact
      241 agaactttaa ttccattttt aacaatgatt caaaatacct tcggttattt gtagctgatg
      301 caggttatgg tagtgagcaa aactatatgg ccattataga tgattttaat aaaacgccac
      361 ttattacgta tggcatgttt attaaagata aaactagaaa gtttaaaagt gacattttca
      421 acactcaaaa ttggaaatat gacgaactta atgatgaatt tatatgtcct aataacaaaa
      481 gaataggttt taaaagatat gcataccgta atgatagata tggttttaaa cgagacttca
      541 aactatatga atgcgatgac tgttcagcat gttctttgag gcaacaatgc atgaaaccaa
      601 attcaaaatc caataagaaa atcatgaaga attataattg ggaatacttt aaagtccaaa
      661 ttaatcaaaa gctttctgaa ccagaaacga aaaaaatcta tagtcaaaga aaaattgatg
      721 tagagcctgt ttttggattt atgaaggcta ttttgggttt aactcgaacg tcagttcgag
      781 gaataaataa agttaaacga gagctaggtt ttgtattaat ggcacttaat ataaggaaaa
      841 tagcagctca acgagctgta cattataaaa tacatatcaa aaaagctgat ttctatcaaa
      901 taattaatag aaatcagctt ttttacattg cctaagaact taatgtccca agccctgaca
      961 attcatctaa ccttttcatc agttgagcga ttttaatcta gccttcaaat ttttttggat
     1021 caggtccaat tctcttatct tgatttaaac catcaattcg cgtcatttgt tcatctgata
     1081 attcgaaatc aaatatttgg aaattttcag agattctgtt tggtgtcacc gatttaggga
     1141 ttgtaaccac agcatgctgc acattccatc ttaaaacaac ttgggcaggt gactttccta
     1201 attcttgagc aatgtcttta attgtctcat catttaaaat ttgtgcattc atcaatggtg
     1261 accaagattc catcacgata cgttgtgctg ccaaatataa tttcaattta tgttgggtta
     1321 aatatggatg atattcaact tgattaatta caggtttaat tgacacttgt gccaacaaag
     1381 cttccaaatg ttcaggttca aaattgctga cacctatatt tttaacatta ttatttttat
     1441 ataaatcttc catacctttc catgtatcaa ccattacggc ttcgttcgta cctggccaat
     1501 gtactagata caaatctaag tattttaaac ctaatctaga taaactagct tcgtaagcag
     1561 ctgctacatt ttcacgaccg aaatcctcaa aatataattt tgaagtaata aataagtctt
     1621 ctctagcaat accagttgac tccaatccgg cacgaatgcc agcacctact tgttcttcat
     1681 tcccataaac ttttgcggta tcaatactac gatatccttg ttcaatggca tacttaacac
     1741 tttccatgca attttcatca ttttccacac gaaatgtccc taaaccaatt tgtggcatcg
     1801 tgtttccatt ataaaatgtt ttaacctcca taaatatcgc ctcacctttt tgatgtatta
     1861 taccctgtta tcataacaaa tctgagttga atacatgaga aaaaacactt agagcaatca
     1921 accactaaaa ttctagtaat atctctcaaa tattaatcaa attgtaaaag taattctgtt
     1981 taatttatga caaactaaaa aagccgaagt aacaacatat agtcatcact tcagcctaac
     2041 atttaattga atgattcaat tttatccatc atttgttgta agtcttccac gttgtattga
     2101 atacgaccat ggaatacaaa tttgttaaag aactcgtcta attgttcagc accgacaagc
     2161 actttgacag cactattttg attataattt gaaatcgtta catcgccttc atttttaaga
     2221 ttaaagtata aaattgaagt tggtgtatat ttggcaccta attctttttg taagtcttca
     2281 gccaattgtt taatcgcctc aatttgatct gaataattta caaatgataa tgaacgtttg
     2341 tcatcatttt gatccatcac aatagtttgc ggtctagatt tatctaaatc caatgtatca
     2401 aatacttgtt ccattggtgg taaatcttta aattgaccgc cactaatacc attataaaca
     2461 tgacctttta acaattgaga atcaataata taaagaccag ttcttgttaa tactaaatga
     2521 ctaattcgtt caatattatt aaagccatcc tttggtaaaa agatatttgc cataatgtgc
     2581 atatcttctg gtcgaattcg tttttcttta actaatcttt cacgaatacc aattaatctc
     2641 atgtccgtta catattcact atgatttttc gagaacaatt ttaatgcgtc aatctcacga
     2701 tcttttgtac taaccatgtg attataatct tcttgttgtt ttgtaattgt ctttttattt
     2761 tgaatacgct ctttctctaa agcttcttca tgagactttt taatgttttg ttcttgttgt
     2821 tcatactttt cttctgtttg tcgcttaact tttttcttac tacctaaggc aactaaaaaa
     2881 aggacaaaaa agattaatgc aatgactact gcaataatga gtccaatgac tatcggtgaa
     2941 gataaatcca tcacaacaac gctccttttt aatatatgaa taactttaat tataatagaa
     3001 aagctaaaga ttttcgatac atattatcat ttatataccg aaaatctttt atttagctat
     3061 attcaattca tcttattatt ttactgcgtc ttttaattct tccactttgt ctaatttttc
     3121 ccatgggaat aagacatctg tacgtccaaa atgaccataa gcagcagttt gtttgtaaat
     3181 cggttgtttc aaatcaagca ttttaataat acctgcaggt cttaggtcaa agtgttttct
     3241 aactgcttcg acaagttgcc cttcagaaac tttacctgtt ccaaatgtat caattgcaat
     3301 tgacactggt tctgcaacac caatcgcata tgccaattgt acttcacatt gatctgctaa
     3361 acctgctgca acaatatttt tagccacata acgtgcagcg tatgcagctg aacggtctac
     3421 ttttgtagga tccttaccac tgaagcatcc gccaccatga cgtgcatagc caccgtacgt
     3481 atcaacaatg attttacgtc ctgttaatcc tgcatcacct tgaggtccac cgattacaaa
     3541 gcgtcctgta ggattgatgt agaatttagt ttgttcatta atcaagtttt ctggaacagt
     3601 tggataaatg acatgtgctt taatgtcttc ttgaatttgt tcaagtgtca catcctcagc
     3661 atgttgtgtt gatacgacaa tcgtatcaat acgtactggg ttatcatttt catcatattc
     3721 aacagtgacc tgaactttac cgtctggtcg taaataattt aacgtaccat ctttacgcac
     3781 atctgataaa cgttttgcca attgatgtga taaataaatt gctagaggca tatacgtctc
     3841 tgtttcattc gttgcgtaac caaacattaa accttggtca cctgcacctg ttgcttcaat
     3901 ttcttcttcg ctatctttat cacgatactc taatgcttta tccacgcctt gtgcaatgtc
     3961 aggtgattgt tcatcaatcg cagttaaaat tgccattgtt tcataatcat aaccatattt
     4021 tgctcttgtg tatccaattt ctttaattgt ttctctaaca actttcggaa tatcaacata
     4081 tgttgttgta gaaatttcgc cggcgatcaa tgccatacct gttgtaacag ttgtttcaca
     4141 agctacacgt gcatttggat cgtcttttaa aatagcatct aatattgcat ctgacacttg
     4201 gtcagcgatt ttatctgggt gtccttctgt aacagactct gaagtaaata atcgtttgtt
     4261 atttaacata gtttgctcct ttaaatttat attacgaaaa ttctctctct gtgagctaaa
     4321 taaaaaagac cttctaacta ttaatataga gagaaggcct aatacgtcca ttcgctctta
     4381 tcgttcagac ctatttgtct gcaaacggtt tggcaccttt cttttataaa aaagaggttg
     4441 ctgggtttca ttgggtccat gtccctccac cactcaggat aagaga
//
LOCUS       DAKTLH010000173         4387 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.177, whole genome shotgun sequence.
ACCESSION   DAKTLH010000173 DAKTLH010000000
VERSION     DAKTLH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.177"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..94)
                     /locus_tag="O0394_002465"
     CDS             complement(<1..94)
                     /locus_tag="O0394_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin MazF"
                     /protein_id="HCY0500519.1"
                     /translation="MTHNIEKRINKLKTSGNPKFKKLDSDIHYLL"
     gene            complement(172..354)
                     /locus_tag="O0394_002466"
     CDS             complement(172..354)
                     /locus_tag="O0394_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500520.1"
                     /translation="MKITNCKIKRETVIYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKIDGDKLTKEDSL"
     gene            complement(558..899)
                     /locus_tag="O0394_002467"
     CDS             complement(558..899)
                     /locus_tag="O0394_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500521.1"
                     /translation="MIKKIFTKKHVFLVIEDENHNHSDAVFGKSILLSIYVGVNKKTN
                     SKSGKFIYLDRSKRIVRQSDITKIESANENDVDFYNLLKKEKEIVYSKNIVDKYNLAN
                     YIIYYEVSTKE"
     gene            complement(905..1837)
                     /locus_tag="O0394_002468"
     CDS             complement(905..1837)
                     /locus_tag="O0394_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCY0500522.1"
                     /translation="MKKYDIAVLDFETMNEHMNSPCEVAVSLIKDLSIVKVYSSYINP
                     PNNRYNLKNARIHKIPEDVILKAPKYPDIYQEILYLLKESHLIIAHNALFDISVLKNT
                     NNYYDLPVPNFMYVDSINIFRSFHAISSFKLENLCSLYDIDKEKLHSAKFDVLALSKM
                     LISLAKNNQHYSVLKLIHYMPKQYIRFSKYSNSPTKLFDSGFQKIHMKISEINKIEVE
                     SVIPILKDKNVVFTGNFDTEKQDLMILTRKKGAYIRSDVTAKTDILVEGVQDDKYKDV
                     NGLVSKQRKAREYVGNGAKIQFLNEEDLINLIKE"
     gene            complement(1853..2566)
                     /locus_tag="O0394_002469"
     CDS             complement(1853..2566)
                     /locus_tag="O0394_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0500523.1"
                     /translation="MNKERNIIIAKNIRKFLNDSNMSQKKLAELINIKPSTLSDYLNL
                     RSNPSHGVIQRIADVFEVGKSDIDTTYKDDNDITSIYNKLTPPRQENVLNYANEQLEE
                     QNSKGDNVVDINSYKQEKTPVNVNGCVSAGVGERLHDETLFTEMVKGPIPTHDLALKV
                     NGDSMEPMFKDGEIIFVEKTHNIKNGQIGIFIIEEEAYVKKVFVEDDRLTLVSLNKDY
                     DDLHFYRNESVRLIGKVIL"
     gene            2529..2702
                     /locus_tag="O0394_002470"
     CDS             2529..2702
                     /locus_tag="O0394_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0500524.1"
                     /translation="MFLAIIIFLSLFILLTSFFLIIRNFHIIVKFFTKKTYLMLTIRK
                     FRIILGLRKAVTT"
     gene            2699..2962
                     /locus_tag="O0394_002471"
     CDS             2699..2962
                     /locus_tag="O0394_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0500525.1"
                     /translation="MKTLKELRTDYGLTQKELGDLFKVSSRTIQNMEKDSTNIKDSLL
                     SKYMSAFNVKYDDIFLGNEYENFVFTNDKKKSIILAFKEKQTS"
     gene            2978..3193
                     /locus_tag="O0394_002472"
     CDS             2978..3193
                     /locus_tag="O0394_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCY0500526.1"
                     /translation="MNIQEATKLAMEKGISIRRENQDVYGILPTNLQRYQCLVVSRHY
                     KKKRQTAAGRWQPSADDLIADDWILDY"
     gene            complement(3182..3511)
                     /locus_tag="O0394_002473"
     CDS             complement(3182..3511)
                     /locus_tag="O0394_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500527.1"
                     /translation="MTNEAKFVLLQLYSIYLDRIDEGMSKRSASYFGSDESSFNAFFL
                     DFNFEDYIDAVLELKHRDFVIASAEDGGFLEMALSREGIAYSESESKKDYKTLMDLIR
                     DLKKLII"
     gene            3562..4314
                     /locus_tag="O0394_002474"
     CDS             3562..4314
                     /locus_tag="O0394_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCY0500528.1"
                     /translation="MQALQTNSNIGEMFNIQEKENGEIAISGRELHQALEVKTAYKDW
                     FPRMLKYGFEENTDYTAIAQKRATAQGNMTHYIDHALTLDTAKEIAMIQRSEPGKRAR
                     QYFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVG
                     ELAKIIKQNGVNIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITH
                     SDGHTSISKTPKVTGKGQQYFVNKFLGEKQTS"
BASE COUNT         1518 a          670 c          668 g         1531 t
ORIGIN      
        1 tgagtaaata gtgaatatct gaatctaatt ttttaaattt tggatttcca gaagttttta
       61 atttattaat gcgtttttct atattatgcg tcatcatttc tcctttattc tcgctcacac
      121 tctcaccacc attcaacgtc tacacttgta gacgtttttt gattagtaaa atcataatga
      181 atcttctttg gttaacttat cgccatctat tttttgtgaa ataaattcca agtatttacg
      241 cgcattatgt gacgataaat ctttaggtaa ctcataagtg aatggttgat taccactagt
      301 taaaacttcg tatattacag tttctctttt tattttgcaa ttagttattt tcattataaa
      361 cttcctttca aacactgctg aaatagacgt cttttttaaa taagcataat taatacttca
      421 attctttaat ccacatatat ttaaaagtga ggtagtaggt aataaatata agacttaaag
      481 ttaagattgc ttttttcatg tttcataatt aaaacctctg taaatttaag gttagtatta
      541 tgaaataatg gattggttta ttctttagta ctaacttcgt agtaaattat atagttcgct
      601 aaattgtatt tatctactat atttttggaa taaacaattt ccttttcttt cttcagtaaa
      661 ttataaaaat ctacatcatt ttcgttagct gattctattt tggtgatatc agattgtcta
      721 acgattcttt tagatctgtc aaggtatata aatttccctg atttagaatt agtcttttta
      781 ttcacaccga cgtaaattga aagtaatata ctttttccaa aaacagcatc actgtgatta
      841 tggttttcat cttctataac taagaataca tgcttttttg taaaaatttt tttgatcatc
      901 gttattattc ctttattaaa tttattaagt cttcttcatt taaaaattga atttttgcac
      961 catttccaac atattctcga gcttttcgtt gttttgaaac tagtccgttc acatctttat
     1021 atttatcatc ttgaacacct tcgactaaaa tatctgtttt tgcagttacg tcacttctga
     1081 tataagctcc tttctttcta gttaatatca ttaaatcttg tttttcagtg tcaaaattac
     1141 ctgtaaaaac aacattttta tcttttaaaa tagggattac actttccact tctattttat
     1201 taatctcaga tattttcata tgaatttttt gaaatcctga atcgaaaagt ttagttggag
     1261 agttagaata tttgctaaat ctaatgtatt gcttaggcat ataatgtatt aattttaata
     1321 cactataatg ctgattgttt ttagcgagtg atatcaacat cttcgataaa gctagcacgt
     1381 caaatttagc agaatgtaat ttttctttat cgatatcata taagctacac aaattttcta
     1441 atttaaaact agagattgcg tggaagcttc taaagatatt tatactatcg acatacatga
     1501 agtttggaac aggtaagtca taataattat tagtattttt taatactgaa atatcaaaaa
     1561 gtgcattatg agcaataatt aaatgtgatt cttttaaaag atagagaatt tcttggtaaa
     1621 tatctggata tttaggtgct tttaatatga catcttcagg tattttatgt attctagcgt
     1681 ttttcaagtt atatctatta ttaggaggat taatataaga tgaataaact tttactattg
     1741 ataaatcctt aattaaagat acagcaactt cgcaagggct gttcatatgt tcattcatag
     1801 tttcaaagtc taagactgca atatcatatt ttttcatttg caagtgctcc ttttataaaa
     1861 taacttttcc aattaacctc acactttcat ttctataaaa gtgtagatcg tcgtaatctt
     1921 tatttagtga aactagagtc aatctatcat cttcaacaaa gactttctta acgtacgctt
     1981 cttcttcaat gatgaatata ccaatttgtc cattctttat attgtgagtt ttctccacaa
     2041 atatgatttc gccatcttta aacataggtt ccatagaatc accatttact tttaacgcta
     2101 aatcgtgtgt ggggataggt cctttaacca tttcagtaaa tagcgtttca tcgtgtaaac
     2161 gttctcctac accagcagag acgcaaccat tgacgttaac tggagttttc tcctgtttat
     2221 atgaattaat atctacaacg ttatctcctt tagaattctg ttcttccaat tgttcatttg
     2281 catagttaag tacgttttct tggcggggag gtgtgagttt gttgtatatg gaagtgatgt
     2341 cgttatcgtc tttgtatgta gtatctatgt cgcttttacc aacctcgaaa acatcagcta
     2401 tcctttgtat aacgccgtga gaggggttgg aacgtaaatt taaataatcg cttaaagtag
     2461 atggttttat gttaatgagt tcagcaagtt tcttttgaga catatttgaa tcgttgagaa
     2521 attttctaat gtttttggct ataataatat ttctttcttt gttcatatta cttacctcct
     2581 tttttcttat tatacgaaat tttcatatca tagtaaagtt ttttacgaaa aaaacgtatt
     2641 taatgttgac aatacgaaaa tttcgtatta tattaggttt acgaaaggcg gtgacaacat
     2701 gaaaacatta aaagagttga ggactgatta cggattgact caaaaagagt taggagattt
     2761 atttaaggtc tcatcacgta caattcaaaa tatggaaaaa gactctacaa acattaaaga
     2821 tagtttactt tctaagtata tgagtgcttt taatgttaaa tatgatgata tttttttagg
     2881 taatgaatac gaaaatttcg tatttacgaa tgataaaaag aaatcaatta ttttagcatt
     2941 taaagaaaaa caaacatctt aataggagga ataacaaatg aacattcaag aagcaacgaa
     3001 gctagcgatg gagaaaggaa taagtataag gagagagaat caagatgtgt atgggatatt
     3061 accaactaat ttgcagcgtt atcaatgcct agtcgtatct agacactata agaaaaaaag
     3121 acaaaccgcc gccggaaggt ggcagcctag cgcagacgat ttaatagcag atgattggat
     3181 tttagattat taattttttc aaatctctaa ttaaatccat aagtgttttg taatcttttt
     3241 tggattctga ttctgagtag gcgatacctt ctcgagaaag agccatctca agaaaaccgc
     3301 catcttcagc agaagcaatt acaaaatctc tatgctttaa ttcaagaact gcatcgatat
     3361 agtcttcaaa attaaaatct aaaaagaaag cgttaaatga ggattcatca ctaccgaaat
     3421 aagatgcaga acgtttagac ataccttcgt caattctatc aaggtaaatt gaataaagtt
     3481 gtaaaaggac aaatttagct tcattagtca taagtcattc acctccttaa taggagtata
     3541 gcagaaagga gcataaacaa tatgcaagca ttacaaacaa attcgaacat cggagaaatg
     3601 ttcaatattc aagaaaaaga aaatggagaa atcgcaatca gcggtcgaga acttcatcaa
     3661 gcattagaag ttaagacagc atataaagat tggtttccaa gaatgcttaa atacggattt
     3721 gaagaaaata cagattacac agctatcgct caaaaaagag caacagctca aggcaatatg
     3781 acacactata ttgaccacgc actcacacta gacactgcaa aagaaatcgc aatgattcaa
     3841 cgtagtgaac ctggtaaacg tgcaagacaa tacttcatcc aagttgaaaa agcatggaac
     3901 agcccagaaa tgattatgca acgtgcttta aaaattgcta acaacacaat caatcaatta
     3961 gaaacaaaga ttgaacgtga taaaccaaaa attgtatttg cagatgcagt agctactact
     4021 aagacatcaa ttttagttgg agagttagca aagatcatta aacaaaacgg tgtaaacatc
     4081 gggcaacgca gattgtttga gtggttacgt caaaacggat tccttattaa acgcaagggt
     4141 gtggattata acatgcctac acagtattca atggaacgtg agttattcga aattaaagaa
     4201 acatcaatca cacattcgga cggtcacaca tcaattagta agacgccaaa agtaacaggc
     4261 aaaggacaac aatactttgt taataagttt ttaggagaaa aacaaacatc ttaaaaggag
     4321 gaataacaaa tgaacgaaga aaaattaaag atgattctct tacttcttga agatatacca
     4381 agagacg
//
LOCUS       DAKTLH010000174         4215 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.178, whole genome shotgun sequence.
ACCESSION   DAKTLH010000174 DAKTLH010000000
VERSION     DAKTLH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.178"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..1017)
                     /locus_tag="O0394_002475"
     CDS             complement(<1..1017)
                     /locus_tag="O0394_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HCY0500529.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFKN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLE"
     gene            1287..1484
                     /locus_tag="O0394_002476"
     CDS             1287..1484
                     /locus_tag="O0394_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500530.1"
                     /translation="MEQIKLKTFTAETLELLEKNINAFLSSEEATNLKLVNITIKEIE
                     ERTFPNNEEEFNAILTLSVNK"
     gene            1542..3023
                     /gene="cls"
                     /locus_tag="O0394_002477"
     CDS             1542..3023
                     /gene="cls"
                     /locus_tag="O0394_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCY0500531.1"
                     /translation="MRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
                     YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLA
                     KDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL"
     gene            3198..4106
                     /locus_tag="O0394_002478"
     CDS             3198..4106
                     /locus_tag="O0394_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0500532.1"
                     /translation="MIKLIQISNINKSFNKRCVLKNISFDIEQGKCIALIGKNGAGKS
                     TLIDILIGNVNANSGEIFDKDKLLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENP
                     LPLEEIIELTKFDSSQLNQFVNKLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEI
                     REYFWSIIENLKEDNRTILYTSHYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQS
                     QITLSDEYIRKLKLDKDDLVIQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKV
                     SIVDSYFNNKKQRGSNYDTKLLENRI"
     gene            4075..>4215
                     /locus_tag="O0394_002479"
     CDS             4075..>4215
                     /locus_tag="O0394_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0500533.1"
                     /translation="MILSYLKIEFKVIMRKKTTLILSILFPVIFYILFTSILELPEDV
                     KPK"
BASE COUNT         1521 a          596 c          645 g         1453 t
ORIGIN      
        1 ttctaatagt ttatttaaat tttcttcggt tgtcgcccaa ctggttgcga atctaacaac
       61 acgatgttga tcatcgtatt tttcccaaac agcaaattta actttttgtt ctaactctgc
      121 tattttctcg ttacttaaaa taaaaaattg ttgattggtt ggagaatcaa agtaaagacg
      181 atagccttta tttttaaacc cgtctttcat cttatttgcc atttcgatag catgtctgct
      241 tatattaaaa tataaattgt ccgtaaataa ttctaaaaat tgtatgcctg ttaaccgtcc
      301 ttttgctaaa agggcaccgt gatgcttgat tcgagtggta aattgtttcg gttcattatt
      361 tttcgtaaaa acaatggctt ccccgcataa tgcacctatc ttcgtaccac ctatataaaa
      421 tacatcacaa tatttagcga tgtctttaat agtcatatct gattggtcac tcatcaatcc
      481 ataccctaat cgtgcaccat ccataaataa tggaagctga tattgcttac atactttgca
      541 taactcttcc aattctgatt tagagtataa tgtgccatat tctgtaggat gagaaatata
      601 taccattcct gggaatacca tatggtcctt tttaaaatca cttttaaatg tctccatgta
      661 agtttcaaca tctgaagcac taacttttcc ttccttagag ggtatagtaa ttactttatg
      721 tccactatat tcaattgcac cgccctcatg cacagcaaca tgaccagtgt ctgctgaaat
      781 gaccccttcg taactttcta acattgaatt aataacaacc tgattggttt gtgttccacc
      841 tactaaaaaa cgaattgtag catttggaca gtcaattgta tctttaatct tttcaattgc
      901 ctgagctgtg aattgatcaa agccatatcc cgaagcttgt acaagatttg tatctactaa
      961 tcgttttaat actttttcat gagcaccttc taaataatca ttttcgaatg aaatcactac
     1021 atttccccct aaaactaata tcaacatttt aataagataa accaatttca aaactagttc
     1081 gatatttaaa atgtattatg aatggttaaa gtttgtatcg cattatcgcg aagttgaata
     1141 aatatattat acatcaaaca atacattatg aaaattaatt aattttttat cacagcgact
     1201 tttcatgctg gaacacatgt attaatgatt gaattttact gttaaaagtt ctatgattta
     1261 ggaaatagag aaaagagggg atttaaatgg aacaaattaa acttaaaact tttacagctg
     1321 agactttaga gttattagaa aaaaacatta atgctttttt aagttctgaa gaagctacaa
     1381 atttaaaatt agtaaatatt actattaaag aaatagaaga aagaacattc ccaaataatg
     1441 aagaagaatt caatgcaatt ttaactttat ctgtgaataa ataatatgac gtgaaaggtg
     1501 caaaatgttt ttttgtgcct ttctttatag gagaaaagac tatgcgattt acattttcaa
     1561 acgatttagg aacgttattt actattattt tagccattgg attcatcatt aatttagtat
     1621 tggcttttat tattatcttt ttagaaagaa ataggcgtac agcgagttca acttgggcat
     1681 ggctatttgt actttttgtc ttaccattga ttggttttat tctttacttg ttttttggta
     1741 gaaccgtttc ggcacgcaaa ttgaataaaa acaatggtaa cgtgttaacg gatttcgatg
     1801 gacttttaaa acaacaaata gaaagctttg ataaaggtaa ttatggtact gataacaaac
     1861 aagttcaaaa acatcatgat ttagtacgta tgcttttgat ggatcaagat ggttttttaa
     1921 ctgaaaataa taaagttgat catttcattg atggaaatga tttatatgat caagttttaa
     1981 aagatattaa aaatgcaaaa gaatatatcc atttagagta ctatactttc gctttagatg
     2041 gtttaggtaa aagaatttta catgctttag aagaaaaatt gaaacaaggt ctagaagtaa
     2101 aaatattata tgatgatgtt ggatctaaaa atgttaagat ggcaaatttt gatcatttta
     2161 aatcgttagg tggagaagtt gaagcatttt ttgcttcaaa attaccgtta ttgaatttca
     2221 gaatgaataa tagaaatcat agaaaaatca tcgtaatcga tggtcaacta ggttatgtcg
     2281 gaggatttaa cattggtgat gaatatctag gattaggaaa attaggatat tggagagata
     2341 cgcatttacg tatacaaggg gatgcggttg atgcactgca gttgcgattt attttagact
     2401 ggaattcgca agcgcaccgt ccacaatttg aatatgatgt taagtatttc cctaaaaaga
     2461 acggaccatt gggcaattca ccaattcaaa tagctgcaag tggcccggct agtgactggc
     2521 atcaaattga atacggttat acaaaaatga ttatgagtgc aaagaaatct gtatatttac
     2581 aatcaccata tttcattccg gataattcat atataaatgc cattaaaatt gctgctaaat
     2641 caggtgtaga tgtacattta atgattccat gtaagccaga tcatccatta gtatattggg
     2701 cgacattttc aaatgcctct gacttattat caagtggtgt taaaatttat acgtatgaaa
     2761 atggatttat acattctaaa atgtgcttaa ttgatgatga aatcgtatca gtgggcacag
     2821 caaatatgga ctttagaagt tttgaattaa attttgaagt aaatgccttt gtatatgatg
     2881 aaaatcttgc taaagattta agggtggctt atgaacatga tattacaaaa tcaaaacaac
     2941 taaccaaaga atcatatgcc aatagaccgc tgtctgttaa attcaaagaa tcgttagcaa
     3001 aattagtttc gccaatttta taatttattt gtaaggagtc tcgattatag aggctctttt
     3061 tattttgccc aaaattaaaa taacgataaa tatattatgt aaatctaata atgacatttg
     3121 tcatttcaaa tctatgacaa agtgttctat tttaaatcta ataaatttac taatattaaa
     3181 ttaagaaaaa aatagaggtg attaaattga ttcaaatatc taatatcaac aagtcattta
     3241 ataaaagatg tgttctaaaa aatatttcgt tcgatattga acaaggtaaa tgtatcgctt
     3301 taattggaaa aaatggtgct ggaaagtcaa cgttaattga tatattaatt ggtaatgtta
     3361 atgctaattc tggtgagata tttgataaag acaagttatt acaaagtgaa aatcgcagta
     3421 taatgttcca aaaaacgatg tttccagatc aattaaaagt tattgagatt atcaacttat
     3481 atcaatcatt ttacgaaaat ccattaccat tggaagaaat aatagaactg acgaaatttg
     3541 attctagtca actgaaccaa tttgtaaata aactttctgg tggtcaacaa cgattactcg
     3601 attttgtatt atctttaatc ggacaaccac aattgatctt attagatgaa ccaacatcga
     3661 ctatggatat agaaattaga gaatattttt ggtcaattat tgaaaattta aaagaagata
     3721 atcgaacgat actctataca tcgcactata ttgaagaagt cgaacgtatg tcagacaaaa
     3781 ttattctcat tgaaaatgga gaaataatac ttaatgattc aacgtcacat attagaacca
     3841 atcagcaatc tcagattacg ttatccgatg aatatataag aaagttaaaa ctagataaag
     3901 atgatttagt tattcaaaaa aatcataatg gcactatcaa aattattact tcaaatgtaa
     3961 atgatacgat tttatatctt caacaacttc atattaattt ggatgatatt gaaatacaaa
     4021 aagtctcaat tgttgattca tacttcaaca ataaaaagca aaggggatct aattatgata
     4081 ctaagttact tgaaaatcga atttaaagtt ataatgcgta aaaaaacaac attaatatta
     4141 tctattttat ttcctgttat attctatata ttatttactt cgatattgga attgccggaa
     4201 gatgttaaac ctaaa
//
LOCUS       DAKTLH010000175         4194 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.179, whole genome shotgun sequence.
ACCESSION   DAKTLH010000175 DAKTLH010000000
VERSION     DAKTLH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.179"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            199..1374
                     /locus_tag="O0394_002480"
     CDS             199..1374
                     /locus_tag="O0394_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HCY0500534.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            1352..2542
                     /locus_tag="O0394_002481"
     CDS             1352..2542
                     /locus_tag="O0394_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0500535.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHILWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIVNR
                     EKAK"
     gene            complement(2655..3164)
                     /locus_tag="O0394_002482"
     CDS             complement(2655..3164)
                     /locus_tag="O0394_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HCY0500536.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIK
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
     gene            complement(3358..4116)
                     /locus_tag="O0394_002483"
     CDS             complement(3358..4116)
                     /locus_tag="O0394_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCY0500537.1"
                     /translation="MSEFKTGKINKHVLYSNILNRDVTLSIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDEK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFDGGHQWKDWKPLLSDILLYFLSKNTDDQLYE"
BASE COUNT         1411 a          683 c          625 g         1475 t
ORIGIN      
        1 atactggata attttaaata tacaaataaa acatagaaca ataaattgct ttataaaata
       61 acgttttatt ttgtatcaat tcatctatag attaaaattg ctataaatgt aacgactttg
      121 cgtctttatt tcttttataa tgccgtaaaa taagttacac ttaaataaaa ttttcaaact
      181 aacggagggt ggctattaat ggttactcaa aataaaaaga tattgattat tactggctca
      241 ttcggtaacg gtcatatgca agttacacag agtatcgtta atcaacttaa tgatatgaat
      301 ctagaccatt taagcgtcat tgagcacgat ttatttatgg aagctcatcc aattttgact
      361 tctatttgta aaaaatggta tatcaatagc tttaaatatt ttagaaatat gtacaaaggg
      421 ttttattaca gccgcccaga taaactagac aaatgttttt acaaatacta tggacttaat
      481 aagttaatta atttattgat aaaagaaaag ccagatttaa tattattaac gtttcctaca
      541 ccagttatgt cggtactaac tgagcaattt aacattaata ttccagttgc tacagtgatg
      601 acagactatc gcttacataa aaactggatt acgccgtatt caacaagata ttatgtggca
      661 acaaaagaaa cgaaacaaga cttcatagac gtaggtattg atccttcaac agttaaagtg
      721 acaggtattc ctattgataa caaatttgaa acgcctatta atcaaaagca gtggttaata
      781 gacaacaact tagatccaga taagcaaact attttaatgt cagctggtgc atttggtgta
      841 tctaaaggtt ttgacacgat gattactgat atattagcga aaagtgcaaa tgcacaagta
      901 gttatgattt gtggtaagag caaagagcta aagcgttctt taacagctaa gtttaaatct
      961 aacgagaatg tattgattct aggttatacc aaacacatga atgaatggat ggcatcaagt
     1021 caacttatga ttacgaaacc tggtggtatc acaataactg aaggtttcgc ccgttgtatt
     1081 ccaatgattt tcctaaatcc tgcacctggt caagagcttg aaaatgccct ttactttgaa
     1141 gaaaaaggtt ttggtaaaat cgctgatact ccagaagaag caataaaaat tgtagcaagt
     1201 ttaactaacg gtaatgagca attaactaac atgattagta caatggaaca agataaaatt
     1261 aagtatgcta cacagacaat atgtcgagac ttacttgatt taatcggtca ttcatcacaa
     1321 ccacaagaga tttatggaaa ggttccttta tatgcaagat tcttcgttaa ataattacgc
     1381 taatcataaa aatttcattt taatgcttat tatcttattt ttaatggaat ttgcgagagg
     1441 catgtatatc ctaagctata ttaatttctt gccaacagtt acatccattg ctgtagcaat
     1501 aacgtctcta gcattttcaa tacattttat tgctgatgca tctacaaact ttgttatagg
     1561 atttttactg aaaaaattcg gcacaaaaat cgttttaacg actggattta ttttagcatt
     1621 tacaagttta tttttagtta tttggtttcc agcatcacct tttgtaatca tatttagtgc
     1681 tatgatgctc ggtattgcag ttagtcctat ttgggtcatt atgttatcca gtgttgaaga
     1741 agataaacgt ggcaaacaaa tgggctatgt ttatttttca tggttgttag gcctattggt
     1801 aggtatggtt tttatgaatc tacttatcaa agtgcaccca acacgctttg ccttcatgat
     1861 gtctttagtc gttttaattg catggatatt atattatttt gtcgatgtaa agttaacaaa
     1921 ttataatact cgtccagtta aagctcaatt gagacaaatt gttgatgtga caaagcgcca
     1981 tttattgttg ttccctggta ttcttttaca aggtgccgca attgctgccc tagttcctat
     2041 attaccaaca tatgctacta aggttattaa cgtaagcaca attgaatata ctgttgcaat
     2101 cattattggt ggtatcggct gtgcagtttc gatgctattt ttatcgaaac taatagataa
     2161 ccgcagcaga aactttatgt atggtgttat tctaagcgga tttattttat acatgatatt
     2221 aatttttact ctatctatga ttgttaatat tcacatcttg tggattatcg ctttagctat
     2281 cggtctaatg tatggcatct tattaccagc atggaatacc tttatggcac gatttatcaa
     2341 atcagatgaa caagaagaaa cttggggtgt ttttaatagt atccaaggat tcggctcaat
     2401 gatagggcca ttatttggcg gattaattac acaatttacc aacaatttaa acaatacatt
     2461 ttatttctcg gcattaatct ttttagtctt agctgtattt tatggaagct actttatagt
     2521 aaatagagaa aaagctaagt aaggttaaaa cgtattttaa aataaagaaa atggtcattt
     2581 ttattacatt caataaaaat gaccattttc ttattaattc aaacatacta cgattaacaa
     2641 tactattatg ttacttaagc taatttaaac gtttcaataa ctttccattt atcttcgtca
     2701 tcgtcaaaac gtaacaaagt taattcatcg ataatttctt tatggtctac cccagctaat
     2761 gctacttgac caaaaatatc ttcgaattct tggctagata gtccttgtgc tattgtaaag
     2821 tgtggcacaa aaacatgttc agcttctcca tagaaatctt ctccattaaa gcgattaaac
     2881 aattcttcta agtcgtccgt cttcgcaact ttaaagtaaa tcacattgtt cgttggtttg
     2941 aagctagaag cttttgtagc atgaacttct actgctggta taccattaat acgagctcta
     3001 acctgttcaa tgacagaatc taaatcacca tctttaattt caaatggcgc tttaattgtc
     3061 acatgtggtt taattcgtga atactgttta tcatatcttt tacggtaaga atccaccgct
     3121 tcttgaaatg actttgatgg aattaatgct aatcctaaaa tcatctgaat tcctcctttg
     3181 tttaacctat tgctattata tcaaatttct ttgaaaaccg ttacattaaa aataattcat
     3241 tatacatctt ttgcaatttc gtcaaattta aattaaatta aacagtaaaa tcttttataa
     3301 attatattat agaccagact atatgaagac aattcatact aaatctacta atgtaaatta
     3361 ttcataaagt tgatcatctg tgtttttact taaaaaatac aataatatat ctgacagcaa
     3421 tggtttccaa tctttccatt ggtgaccacc atcaaattca tcgtaataat aagttatatt
     3481 atatttctta atttgttcag ctaattctct attcggtgtt aagaaatcgg cacgcttacc
     3541 atttgttggt aaagtaaaat ctttttcatc tagaccaatg acatgccaaa ttgtcaattg
     3601 ttctttattt gcacattgat ttagcttatc taatactttt tcatctgaat gtggacttaa
     3661 cattgctaca cgactaaaaa tcgttggata ggtcaacgcc gttaataagg caatactacc
     3721 cgctaaacta tcccctacta ataaccttgc attacctact ttcagtgtag aaaacgtcga
     3781 gtcaataaat ggcaatattt ctttaccgac tgattgaata gttaaatgag aacgacttcc
     3841 ttgtggatga aattcctctc tacgcttatc aacgtcttca taatggaatc caacaatgat
     3901 cgcatcatca atacgcgctt ctttgattaa cgattcatat gtacgttgta ttctcccgaa
     3961 acgtaaaaaa tctaatccgt caaagcaaag aatgacatta tatttaacaa gttgattata
     4021 agattctggt aaataaatac ttaacgtgac atctctattt aaaatattac tatataaaac
     4081 atgtttatta atcttaccag ttttaaattc tgacataaaa aagtgcccct agtagttgta
     4141 ttgttattgt accaagagac actatatttt taaaattcaa atttctgagc acct
//
LOCUS       DAKTLH010000176         4148 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.180, whole genome shotgun sequence.
ACCESSION   DAKTLH010000176 DAKTLH010000000
VERSION     DAKTLH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.180"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..623
                     /locus_tag="O0394_002484"
     CDS             <1..623
                     /locus_tag="O0394_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCY0500538.1"
                     /translation="VIYIQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWA
                     LLGFALVMIYQMVVSIVLTQIYGGQQVSPNTEKLIIIARKIPIFIFFVSIIGPLLEEY
                     VFRKVIFGELFNAIKGNRIVAFIIATTVSSLIFALAHNDFKFIPVYFGMGVIFSLAYV
                     WTKRLAVPIIIHMLQNGFVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            complement(648..1901)
                     /locus_tag="O0394_002485"
     CDS             complement(648..1901)
                     /locus_tag="O0394_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500539.1"
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATHTVEAKDNLNGEKPTTN
                     LNHNITSPSVNSEMNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPST
                     DSKPDPNNQNPSPNPKPDPDNPKPKPDPKPDPDKPKPNPDPKPDPDNPKPNPDPKPDP
                     NKPNPDPKPDPDKPKPNPNPKPDPNKPNPNPSPDPDQPGDSNHSGGSKNGGTWNPNAS
                     DGSNQGQWQPNGNQGNSQNPTGNDFVSQRFLALANGAYKYNPYILNQINKLGKDYGEV
                     TDEDIYNIIRKQNFSGNAYLNGLQQQSNYFRFQYFNPLKSERYYRNLDEQVLALITGE
                     IGSMPDLKKPEDKPDSKQRSFEPHEKDDFTVVKKQEDNKKSASTAYSKSWLAIVCSMM
                     VVFSIMLFLFVKRNKKKNKNESQRR"
     gene            2098..2724
                     /locus_tag="O0394_002486"
     CDS             2098..2724
                     /locus_tag="O0394_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCY0500540.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
     gene            3085..3870
                     /locus_tag="O0394_002487"
     CDS             3085..3870
                     /locus_tag="O0394_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HCY0500541.1"
                     /translation="MKVQIYQLPIVFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGEYVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
BASE COUNT         1313 a          629 c          718 g         1488 t
ORIGIN      
        1 cagtcattta catacaactt gttctatttt taatcgccgc cacgacgatt attttaatta
       61 atttaaaaat taaaaatcca acaaaattag aattagaagt taaagaacct aaaaaatata
      121 tcattccatg ggcattgctt ggatttgcat tggtaatgat ttatcaaatg gtagtgagca
      181 ttgtattaac gcaaatttat ggtggacaac aagtaagtcc taatacagaa aagctaatta
      241 ttattgctcg aaaaatacct atatttatct tctttgtatc tattattggt cctttattag
      301 aagaatatgt attcagaaaa gtaatctttg gagaattatt taatgcgatt aaaggtaatc
      361 gtatcgtggc atttattatt gctacaacag taagttcatt aatatttgca ttagcacata
      421 atgatttcaa atttattcca gtttattttg gtatgggtgt cattttttca ttagcatatg
      481 tttggacaaa acggcttgct gttccaatta ttatccatat gttacaaaac ggatttgtcg
      541 ttatattcca attacttaat cctgaggctt tgaaaaaagc cacggaacaa gcgaatttta
      601 tatatcacat ttttattcca taataaaaaa aacgaatcac acacggatta tcgtcgctgt
      661 gattcgtttt tatttttctt tttatttcgc tttacaaata agaatagcat gattgaaaat
      721 accaccatca tagaacatac aattgctagc caacttttac tatatgcagt tgacgcactt
      781 ttcttattat cttcttgttt tttaactact gtaaaatcgt ctttttcatg cggttcaaat
      841 gagcgttgtt ttgaatccgg cttatcttcg ggctttttca aatctggcat tgatccaatt
      901 tcaccagtaa ttaatgcgag tacttgttca tctaaattac gatagtacct ttctgatttc
      961 aatggattga aatattggaa tctaaagtaa ttcgattgct gttgtaatcc atttaaatat
     1021 gcatttccgc tgaaattttg ttttcgaata atattataaa tgtcttcatc agtaacttct
     1081 ccataatctt tgcccaactt attaatttga tttaaaatat acggattata cttgtaagcc
     1141 ccatttgcca aggctaaaaa tcgttgggat acaaaatcat taccagtagg attttgtgag
     1201 tttccttgat tcccatttgg ttgccattga ccttgattag atccatctga agcatttggg
     1261 ttccatgtcc ccccattttt cgagccacca gaatgattgg aatccccagg ttgatcggga
     1321 tctggtgacg ggttaggatt tggcttatta gggtctggtt ttggattcgg atttggcttt
     1381 ggtttatctg gatctggttt tggatccgga tttggcttat tagggtctgg ttttggatct
     1441 ggatttggtt tcgggttatc tggatctggt tttggatccg gatttggctt tggtttatct
     1501 gggtctggtt ttggatccgg ttttggtttc gggttatctg gatctggttt aggattcgga
     1561 cttgggtttt ggttatttgg gtctggtttt gaatctgtac ttgggttttg gttgtttgag
     1621 tctggcttca cattattcga atcaggatta gcatcacgac tattcgaacc tgttccttca
     1681 ttacccgttt gatttgattc gtgaggtgtc ccagtctcat tattattcat ttcactattt
     1741 actgatggtg aagttatatt atgattcaaa ttagtagttg gtttttctcc atttaagtta
     1801 tcctttgctt caactgtgtg cgttgcaagc cctaaactac tactacttaa aattaaaata
     1861 cttgttgaaa gtaacatttt cttaaaccaa tgatatgaca tttcacgaga cacctccctt
     1921 tttcatgtaa gtattaatct aagtataata aatatgcgtt gaaaatgcat ttatatttgg
     1981 aatttgaaaa tgaatattaa tattcttgaa attttcgtaa atattgcttt acaagtacga
     2041 gtataaattt tatacttagt ctattaagta ttctttacta aagaaggagc aataattatg
     2101 ggcttattta aaaaagataa aaaagcaatg acttttgaca atgcaatgga agaaagacgt
     2161 tcaatatata atttgaaaga ttcgatatca attagcgatg acgaattaga atcaatgatt
     2221 gcacatgcag ttaaacatgt tccttcatca tttaattctc aatcaactag aattgttctc
     2281 ttgttaaatg acaacaataa taaattttgg gataacacaa aagcaatttt aaaagaagtc
     2341 atgggagaga atcgtgattt cgagccaact gaacaaaaaa ttgataactt caaacattca
     2401 tacggtacaa ttttgtttta tgaagatcaa gatgttgtaa gtggtttaca agaacaaatg
     2461 ccaaactact atgataattt tgcaatatgg tcaactcaaa caaatgccat gcatcaattt
     2521 gcaatttgga ctgcattagc tactaaaggc ataggtgctt cattacaaca ctataatcca
     2581 cttgttgatg agatgacttc aaatgaattt aatattccta aaagttggaa acttattgct
     2641 caaatgccat ttggtgatat tcgtgaagct gctggcgaaa aaacatttaa tccagtggaa
     2701 gatcgtttcg taataaaaaa ataatcaata tattgttagg aatgggacag aaatgataaa
     2761 gaatcattag ttattcatta tgtagtagtt cttacacatt agccgctgct aatgtgcact
     2821 taagaatagg aatacatgag taaaactcat gtataagaaa tactaatttc taaagaaaaa
     2881 gtatttcttt atgttagagc ccaccccaac ttgcattgtc tgtagaaatt ggtgttccaa
     2941 tttctctatg ttggggcccc atcaactact gtcaatataa ctttgtagag cctaggactt
     3001 tgattttatg tcttgtgctc gttttttgcg tttcaacata acttttagta agttatatat
     3061 aagatctatc aaggatgtga tgttatgaaa gtccaaattt atcaattacc gattgttttt
     3121 ggagatagtt ctaaaaatga aactcaaata acgcaatggt tcgaaaaaaa tatgaatgct
     3181 gaagtagatg tagtcgtttt acctgaaatg tggaataatg gctatgattt agagcattta
     3241 aatgaaaaag cagataataa tctaggacaa agtttttcat ttattaaaca tttagctgaa
     3301 aaatataaag tcgatatcgt agctgggtca gtttctaaca ttagaaacaa tcaaatattt
     3361 aatacagctt ttagtgtgaa taaaagtggc cagctgatta atgaatacga caaagttcat
     3421 ttagttccta tgttaagaga gcatgaattt ttaaccgctg gcgaatacgt agctgaaccc
     3481 tttcaattgt ctgacggtac ttatgtgacc caacttatct gttatgattt acgttttcca
     3541 gaattattaa gatatcctgc tcgtagtggt gctaaaattg cattttatgt ggcgcaatgg
     3601 cctatgtcac gtcttcaaca ttggcattca ttactaaagg cacgtgcaat tgaaaataat
     3661 atgtttgtca ttggaactaa tagcactgga ttcgatggta acacagaata tgctggtcat
     3721 tcgatagtta taaacccaaa tggagattta gttggagaat taaatgaatc cgcagatatt
     3781 ttgactgtag atttaaactt aaatgaagta gaacagcaac gcgaaaatat acctgtattt
     3841 aaaagtatta aattagattt gtataaataa gaagcgcccg aaataatatt taacacatgt
     3901 catgactaaa catagattta tgagagattt ttcataagtc tatgtttatt tttttataat
     3961 agataactca gtaagaaccc atttcgccct ttgcaaatga atgattttct taattaaatt
     4021 gattaatttt ttgacctttt ccaacattag acttattcat atattttaac ggcgggtctc
     4081 ataatgatga ttaactcatc ccttcttcat tacaaaaaag gccgcgagct tgggaggggc
     4141 tcacgacc
//
LOCUS       DAKTLH010000177         4111 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.181, whole genome shotgun sequence.
ACCESSION   DAKTLH010000177 DAKTLH010000000
VERSION     DAKTLH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.181"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(262..594)
                     /locus_tag="O0394_002488"
     CDS             complement(262..594)
                     /locus_tag="O0394_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCY0500542.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
     gene            complement(678..1823)
                     /locus_tag="O0394_002489"
     CDS             complement(678..1823)
                     /locus_tag="O0394_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCY0500543.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            complement(2032..2411)
                     /gene="rnpB"
                     /locus_tag="O0394_002490"
     ncRNA           complement(2032..2411)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="O0394_002490"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(2474..2818)
                     /gene="gpsB"
                     /locus_tag="O0394_002491"
     CDS             complement(2474..2818)
                     /gene="gpsB"
                     /locus_tag="O0394_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCY0500544.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
     gene            complement(2832..3395)
                     /locus_tag="O0394_002492"
     CDS             complement(2832..3395)
                     /locus_tag="O0394_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCY0500545.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            complement(3388..3738)
                     /locus_tag="O0394_002493"
     CDS             complement(3388..3738)
                     /locus_tag="O0394_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HCY0500546.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     ILNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTDG"
     gene            complement(3793..3972)
                     /locus_tag="O0394_002494"
     CDS             complement(3793..3972)
                     /locus_tag="O0394_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001788819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500547.1"
                     /translation="MLLISRGVFDLNDFIRLKVFSSYNNIHDKILYLYRKFLDLLDLT
                     DNTFEKIVKSNYIEC"
BASE COUNT         1282 a          762 c          525 g         1542 t
ORIGIN      
        1 ttgtatttat aattgaatta atattatata ttaaagatcg tcgtgctaaa caataatgca
       61 cttaaagttt tgaactgacg aaatttacaa aatgattctc gtctctctaa ttacttaaaa
      121 cgggttctat aataaatcgt actgatggaa agtttttact ttttatctgt ccgatttttt
      181 gaatttgaag ataaaaaaac atctaaaacg cataagaaga taactctact tcttcaaaat
      241 tacgttatag atgctttttt attaattcat atgcatgtcg tgaattttca tcattaaacc
      301 gtgtgggacg tcatcatgtt taacaatctc ttgtttataa aatgtcttcc catcatcttt
      361 agaaactaat tttacaaagt ctcctttttt cggcttgaaa tcattagatg gttctacttt
      421 gatattatgt ttgactaatc catctttttc actgacaact ttttctgcat ttgtattact
      481 attcatataa ccaaaataag tcgttttttg actagaaaaa atcattaaac atacaacggc
      541 aataccaata acaaccaatg caatagataa acctagaccc atttttttac ccatttatag
      601 actccttcat ttttcaaaaa tacctattat catgagaact taattattac taattttagt
      661 cacgcattag aaacacttta gttttcaata gttttacgtt ctgtcttttt accccagtat
      721 tgataataag tacattctat atatccatta aataacttac gtcgctttgt tgctttacga
      781 tctactaaat attcaaattc tttattactt gttaaaatgt atgtagataa aaatggatgt
      841 tgtttcatta gtttaccaat ataacggtac atttcttcaa cttcttcacg atcaccaata
      901 cgttcaccat atggaggatt tccaattaac gccaccggtt cttctgtatc aattgttaat
      961 gtattgacat cttttacact aaatttaata atatcagaca atccaacttc ttcagcgtta
     1021 cgcttagcaa tctctaccat ttctggatcg atatcagaag catatacttc gatttcttta
     1081 tcataatcag ccatcttatc cgcttcatca cggtaatcat cataaatatt tgctggcatg
     1141 atgttccatt gctctgatac gaactcgcga ttaaaaccag gtgcgatatt ttgagcaatt
     1201 aaacaagctt ctatagctat tgttcccgaa ccgcaaaatg gatcaattaa aggtgtatca
     1261 cctttccagt ttgcaagacg gattaaactt gctgccaacg tttctttaat tggtgcttca
     1321 ccttgtgcta atctataacc acgtctgttc aaaccagaac ctgatgtgtc gatagtcaat
     1381 aatacattat cttttaaaat ggcaacttca acagggtatt tggcacctga ttcatttaac
     1441 caaccttttt cgttatatgc gcgacgtaat cgttcaacaa tagctttctt agttatcgcc
     1501 tgacaatctg gcacactatg tagtgttgat ttaacgcttc taccttgaac tgggaagtta
     1561 ccctctttat caattataga ttcccaaggg agcgctttgg tttgttcgaa taattcgtca
     1621 aacgttgttg cgttaaaacg tccaacaaca attttgattc ggtctgctgt gcgcaaccat
     1681 aaatttgcct ttacaattgc acttgcgtct ccttcaaaaa atatacgacc attttcaaca
     1741 tttgtttcat agcctaattc ttgaatttcc ctagcaacaa cagcttctaa tcccatcgga
     1801 caaactgcaa gtaattgaaa catatatgat tctcctttta tacaggtatt ttattcttag
     1861 cttgtgtttt ttatacattt ccaacaaatt taatcgctga tacattaacg catccgctta
     1921 ctattttaaa acaaggcagt gtcattatat caagacaagg cgttaatttt aagtgttctt
     1981 ttcatgaaaa aagctctcca tcatctagga gagctaaact agtagtgata tttctataag
     2041 ccatgttctg ttccatcgta ctcatcacgt gcactagtca cactggtact caggtgataa
     2101 ccatctgtct acaccacttc atttcgcgaa gtgtgtctcg tttatacgtt gaattccgtt
     2161 aaacaagtgc tcctaccaaa tttggattgc tcactcgagg ggtttaccgc gttccacctt
     2221 ttatatttct ataaaagcta cgtcactgtg gcactttcaa attactctat ccatatcgaa
     2281 agacttagga tatttcattg ccgtcaaatt aatgccttga tttattgttt catcaagcac
     2341 gaacactaca atcatctcag actgtgtgag catggacttt cctctatata atatagcgat
     2401 tacccaaaat atcactttta aaattataac atagtcatta ttagtaagac agttaaactt
     2461 ttgtatttag taattattta ccaaatacag ctttttctaa gtttgaaata cgttttaaaa
     2521 tatctacatt atttgaagat gtatttgttg ttgtattatt cgaagaaaaa cttttattgt
     2581 cctgaggtct tgatgttgct acacgtagtc ttaattcttc taattctttt ttaagtttat
     2641 gattctcttc tgataatttt acaacttcat tattcatatc ggccattttt tgataatcag
     2701 caataatgtc atctaaaaat gcatctactt cttctcttct atagccacga gccatcgttt
     2761 tttcaaaatc tttttcataa atatcttttg ctgataattt caatgaaaca tctgacattt
     2821 tttccacctc attagaaact ttgatcttca gaccactgta agtcattgat gaatgctgtt
     2881 aattcatcga acgtcacaat atcacaagta tagtttgttt tatccataaa atcaactaac
     2941 atctgcttga agaacttagg actcgcctct tgttcctcat cataaatgag caatgtttga
     3001 tccgaatgtt caagcataaa ttgatctgct tgtttaaatt gaaaaggacc ttgatacgac
     3061 gtatgaaaaa tactatcaac ataatctgca tgcttaatta tgttggcata cttactttga
     3121 ttatgttcat tccacttttc tgtatgtcct tggaacggtg taattacagc aaactttaac
     3181 gaatcatatg ttcgttgtaa ttcaataaca acttctgcag tccataattc tatacccatt
     3241 tgcccttgta ttaacaccca ttctaatcct tcatccaaca gttgttcaat tttatgtttt
     3301 ataaattgtt ttaaataatg tacttcaggt gcgtcatctt taaaaatgtt taattcgaat
     3361 gatttgtaac ctgttacata aactgtttta accatcagta ccctcttttg ttacgccatc
     3421 taatatattt tgtaaatcat attgaacact tttaagcttt tctataaata actttcgact
     3481 tgttcgctta aaatgacatt cgacagaaag ttgttcaatg ttagcaatca gtagctcaaa
     3541 tttccttgaa ttcatataag gtacttctat aataaattca cgatgtaatt tgatctcatt
     3601 gagtatgcta tcaatatgtt cagtatatgg ctttacagtt tggtaaaaat catgatcttg
     3661 ttgttgtgat ttcacatttt caaaattttg ttgcatgttg ttaacttcat aaattagtga
     3721 ttcgactaca tcattcatga ggacgcctcc tacatttttt aatttatcac aatatactgt
     3781 attcgtcatg ttttaacact ctatataatt tgatttaact attttttcaa atgtgttatc
     3841 tgttaaatca agtaaatcta aaaacttcct atataaatac aaaattttat cgtgtatgtt
     3901 gttatacgat gaaaatactt ttaatctaat aaaatcattt aaatcaaata cacctctgct
     3961 gattaacaac acatacttgt acttgcctca aaaataaaaa ttactaatca tgatttgact
     4021 tttataacaa aattcaaaaa tattgtaatg agtattcatt tttataagca aaacttttca
     4081 attaattaaa attgtgtgta aaattccaac a
//
LOCUS       DAKTLH010000178         4078 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.182, whole genome shotgun sequence.
ACCESSION   DAKTLH010000178 DAKTLH010000000
VERSION     DAKTLH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.182"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            161..262
                     /locus_tag="O0394_002495"
     CDS             161..262
                     /locus_tag="O0394_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500548.1"
                     /translation="MKFAANKLTLTIFRKFCINNEKEIWIKYEEVFL"
     gene            272..544
                     /locus_tag="O0394_002496"
     CDS             272..544
                     /locus_tag="O0394_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HCY0500549.1"
                     /translation="MFGTILYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASATKHNWWVNRAYYNIY"
     gene            588..2552
                     /locus_tag="O0394_002497"
     CDS             588..2552
                     /locus_tag="O0394_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCY0500550.1"
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRIDQFAKNENVAIYKSTSNYTNKNVDKDIYVFNKSKAATITPFNAKY
                     NIHYLSDDEVLKKDIKGSYFVKDKNFDVSKFINFLKEYGVTAESYKIDHMMIAVGVIK
                     QMNIVVPLSSLLIVYFIYYIFEKNINFKAYAIKYLNGFTLRKIIFENFSKKCTYWVTL
                     IITQILLTTSVLWILNYTGNLDLFILRIVLLSCLFILTISVINLWTFLMLLNLNIANM
                     IKGKQHFKTIRFINTVCKSILLVLIANVMIENTSVIKDLNKIKETEKYWNVLDDYYTI
                     EFAPYHETKQSLIDNMVRSEQLVKASEAENNAILFKPKGDSVDNDNFSPDEGNVILVN
                     NQFWSIYYKQFQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAYHSWFEFVQNKNNKEN
                     KLSIQFINKNDCRIFSFDARDSRHLSFIEAPIIVNVQASDLSNDFYYAMISQGGYLFK
                     NYDALVKNIEKYHLDGEISGITNYKDSVMEMYHENNLKLTVLNFSQIIIAIILIIIIL
                     FDVKYYFEQHRKLLVIKKLYGYSTLRANYQYLLINNIVVIFIGILTNVILHSHYIMML
                     FATILVVQILLQICSLYYHGRRFNEVIKEF"
     gene            2555..2875
                     /locus_tag="O0394_002498"
     CDS             2555..2875
                     /locus_tag="O0394_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCY0500551.1"
                     /translation="MKFIIAILLGLILSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDTPKE
                     VRQE"
     gene            2872..3513
                     /locus_tag="O0394_002499"
     CDS             2872..3513
                     /locus_tag="O0394_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0500552.1"
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIDNLTVNENLDIGLAYK
                     KISKKEKEQIKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPT
                     GALDPKTGQMIIQSLFGLVDENKVLILATHDMAIANQCDEIIDLEQYRKVASM"
     gene            complement(3601..3891)
                     /locus_tag="O0394_002500"
     CDS             complement(3601..3891)
                     /locus_tag="O0394_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500553.1"
                     /translation="MFYACHFKVKIGGVILTRTYNIIGILSCLISFIIMALPMIWYTA
                     SALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLIVLNIIILLFFSLPLLLS"
BASE COUNT         1607 a          501 c          634 g         1336 t
ORIGIN      
        1 ccaaactttg acaacgaatg atggttaaag aaaatctcat ttagtcaccg tctttttaac
       61 gggctcaata ggcgacatgt tcctgcatat cgtctattta tacactgcac aatttaataa
      121 taatacaatt atactttgat gtccatttaa cataaagtgg ttgaaatttg cagcaaataa
      181 attaaccttg actattttca gaaaattctg tataaataac gaaaaagaga tatggattaa
      241 atatgaagag gtgtttttat agaagaataa aatttttggc actatattat atttaacttt
      301 agcacttgga ttatcaacag cagcttatgc atctacagaa tacgcagaag gaggcacttg
      361 gagtcatggt gtcggcagta agtatgtttg gtcgtattat tatcatggtc ataaaggaca
      421 tggtgcaaca gctattggaa aatatagatc atttagtggt tatacaagag ctggtgtaaa
      481 agcaaaagca tcagctacta aacataattg gtgggtcaat agagcgtatt ataacattta
      541 ttaaatacta aaaaatcata gaaaataaac aacgcaagag gtacaacatg aaatggttca
      601 aattaatact tgatgttaca atcttcattc tgattgctat attgttattt gtttatacat
      661 ataaagaaaa cgaagaaata ttgcctgata ctaaataccc tatagcggta actgactgga
      721 ataaaaaata tagtaaaaat gagatttata aacgtataga tcaattcgct aaaaatgaga
      781 acgtagcaat ctataaatca acttcaaatt atacaaacaa aaacgtagat aaagatatat
      841 atgtatttaa taaatcaaaa gcagcaacta tcactccttt taatgctaaa tataacattc
      901 attatttaag tgacgacgaa gtattaaaaa aagatatcaa agggagttat tttgtaaaag
      961 acaaaaattt tgacgtgtct aaattcataa attttttaaa agaatatggt gttactgctg
     1021 aatcatacaa aatagatcat atgatgattg ccgttggtgt cattaaacaa atgaatatag
     1081 tagttccatt atcctcactt ttaatcgttt attttattta ttatattttc gaaaagaaca
     1141 ttaatttcaa agcgtatgcg attaagtatt taaatggttt tacattaaga aaaataattt
     1201 tcgaaaattt ttcaaagaaa tgcacgtatt gggtaacgtt aatcataact caaattcttt
     1261 taactacaag tgtactttgg atattaaatt acacgggtaa tttagattta ttcatattaa
     1321 gaatagttct gctttcatgt cttttcattt taacgattag tgttattaat ttatggactt
     1381 tcttaatgtt actaaattta aatattgcaa atatgattaa aggtaagcag cactttaaaa
     1441 caattcgttt tattaataca gtttgtaaaa gtattctctt agtactaata gctaatgtaa
     1501 tgatagaaaa tactagtgtt attaaagatt tgaataaaat aaaagaaact gagaaatatt
     1561 ggaatgtatt agatgattat tacacgattg aatttgcacc ttatcacgaa acaaaacaaa
     1621 gtttgattga taatatggtg cgatcagaac aattagtaaa ggctagtgaa gcagaaaata
     1681 atgcgatttt attcaaacca aagggtgact ccgttgacaa tgacaacttt tcgcctgatg
     1741 aggggaatgt aatattagta aataatcagt tctggtcgat ttattacaag cagtttcaac
     1801 ctgatattcc gataaaaaat caaaaaaata atgtcgaagt aattattcca caaaagtttc
     1861 atgcaatgcg taatgaaatc aatcaagcat atcattcatg gtttgaattt gtacaaaata
     1921 aaaataataa agagaataag ttatctatac agtttatcaa caaaaatgat tgtcgaattt
     1981 tttcatttga tgcacgagat agtcgccatt tgtcatttat agaggcgcca atcattgtga
     2041 atgttcaggc atcagattta tcgaatgatt tttattatgc catgatcagt caaggcgggt
     2101 atttattcaa aaattatgac gcgctagtaa aaaatattga aaagtatcat cttgatgggg
     2161 aaatcagtgg aataaccaat tataaagata gcgtgatgga aatgtatcat gaaaacaatt
     2221 tgaaattaac agtactcaac ttttcacaaa tcattatcgc aatcatttta ataattatta
     2281 ttttatttga tgtgaaatat tattttgaac agcatcgaaa attactcgta atcaaaaagc
     2341 tatatggtta ttcaacatta agagccaatt accaatactt attaataaat aatatagttg
     2401 ttatttttat tggaatattg acgaatgtaa ttttacattc tcactatata atgatgttat
     2461 ttgcaacgat tcttgtcgtt caaatattat tgcagatttg cagcctatac tatcatggcc
     2521 gacgttttaa tgaagttatc aaggagtttt aactatgaaa tttatcattg caatattatt
     2581 aggattaata ttatccatta ctattgcttt tacaatcata catcatccta tacttgatcg
     2641 ttttaatcct ttcttaaaaa cggagtatag ttatgccaaa gtgccaaaag gtacgcaaca
     2701 atatgttaat attacggctt atagtgaaag aggggaaaag cttgattata aattaacatt
     2761 taatggattt tcacctagta gaacgtatgt cgaaataaag cataaagggc aatatgtcat
     2821 atcgatcaca tatgttgaaa aagaggatac accaaaagag gtaagacaag aatgatagaa
     2881 ttaaaagatt taaccataca aaaaggtaat acacatattt tgaagaaact taatttgaaa
     2941 tttcaatgtg gaaaatcata tgcacttatt ggtaaaagtg gatgcggtaa atctacatta
     3001 ttaaatacta ttgctggact tgaaaaaaca ggaaaacaat atgtctattt taatggtcaa
     3061 ttagaacaat ttaaatctaa tttttacaga gataaattag gatatttatt tcaaaattat
     3121 ggattaatcg ataatttgac agtaaatgaa aatttagata ttggattagc atataaaaaa
     3181 ataagtaaga aagaaaaaga acaaataaag atacgttata tagaacagtt tggtctgtca
     3241 aacagtttaa aaagaaaagt tcatacgcta agtggaggtg aacaacaacg tgtcgcttta
     3301 attagaatga tgttaaaaga tccgattgtt atgttagctg atgaaccaac gggtgcgtta
     3361 gatcctaaaa caggacagat gattattcaa tcattatttg gtttggtcga tgaaaataaa
     3421 gtgctgattt tagcaacaca tgatatggct attgcaaatc aatgtgacga aataatagat
     3481 ttagaacagt atagaaaagt agcatctatg tgatatccaa aagataaaat taaaagccaa
     3541 tcaatgggaa atgttgactt caacactccg tttgattggc ttttatactg tgttactaag
     3601 ttatgaaagt aacaacggca aactgaaaaa aagcaatata ataatattta atacgattag
     3661 taataaatgt aattgatttt tcgttttaat atagcaaaaa acaatgacta aagataacaa
     3721 caatatcatg attgcacctg ggaagaacca taatgctgaa gctgtgtacc aaatcattgg
     3781 taacgccata ataataaaag atataagaca agaaaggata ccaataatat tataagttct
     3841 agtcaaaatt acacctccaa ttttcacttt aaaatgacat gcgtaaaaca atcattttaa
     3901 atacattata caataaaatt aaattgtgcg gtagaagaat ttgaaattaa attgaattgt
     3961 ttttggtagt tggtatattt ataacactct gcaaaagaat gtagcaaaat tagataaatt
     4021 agattattta aaattatgcg tgatattatt atttttatca tgcaaataat tataataa
//
LOCUS       DAKTLH010000179         4023 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.183, whole genome shotgun sequence.
ACCESSION   DAKTLH010000179 DAKTLH010000000
VERSION     DAKTLH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.183"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..1631
                     /locus_tag="O0394_002501"
     CDS             <1..1631
                     /locus_tag="O0394_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCY0500554.1"
                     /translation="KKLTTILFQYKIFPVLMFLVSTGLGIIVITQNILIADFLAKIIR
                     HQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGEQ
                     MTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLFY
                     IIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRTL
                     TMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYNA
                     IKDLGQAFHTGKQSEGASDVVFEFLEQPNYNNEFLLKYEENQKPFIQLTDISFRYDDS
                     DRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNQRDLNIGIL
                     SQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTKGINTIIGEGGEMLS
                     GGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRDN
                     TIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            1628..3301
                     /locus_tag="O0394_002502"
     CDS             1628..3301
                     /locus_tag="O0394_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HCY0500555.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKKRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSVRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAITRMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(3428..3871)
                     /gene="mgrA"
                     /locus_tag="O0394_002503"
     CDS             complement(3428..3871)
                     /gene="mgrA"
                     /locus_tag="O0394_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HCY0500556.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
BASE COUNT         1332 a          573 c          718 g         1400 t
ORIGIN      
        1 tgaaaaaatt aacaacaata ctgtttcaat ataaaatttt tccggtactc atgttcttgg
       61 tcagtactgg tctcggcata atcgttataa cgcaaaatat tttaatagca gattttttag
      121 ctaaaattat aagacatcaa tttcaaggtt tatggattgt attatttatt ttattaggtg
      181 ttttactttt aagagcaact gtgcaatttc taaatcaatg gttaggtgat acattagcat
      241 ttaaagttaa gcatatgctt agacagcggg ttatttataa aaataatggt catccaatcg
      301 gtgaacaaat gactatactc acagaaaaca ttgatggtct agcacctttt tataagagtt
      361 atttgcctca agtgttcaaa tcaatgatgg ttccgctcat cataatcatt gcaatgtttt
      421 tcatccattt caataccgca ttaattatgt taataactgc accatttatt cctttgtttt
      481 atattatttt cggtttgaaa acgcgagatg agtcaaaaga tcaaatgact tatttgaatc
      541 aatttagtca acggttttta aatattgcta aaggtttagt gacgttaaag ctatttaatc
      601 gtacagagca aacagagaag catatttacg acgatagtac tcagtttaga actttaacaa
      661 tgcgcatttt acgcagtgct tttttatcgg gattaatgct cgaatttata agtatgttag
      721 gtattggatt ggttgcattg gaagcaacgc taagcttagt agtatttcat aatattgatt
      781 ttaaaactgc ggcaattgcg attattttag cgcctgaatt ttataatgca attaaggact
      841 tagggcaagc gttccatact ggaaaacaaa gtgaaggtgc cagtgacgtt gtgtttgagt
      901 ttttagaaca accgaactat aataatgaat ttctattaaa gtatgaggaa aaccaaaagc
      961 catttattca gttaacagac atatcatttc gatatgatga ttctgataga ttggtattaa
     1021 atgatttaaa tttggaaata tttaaaggtg atcaaattgc acttgtaggt ccaagcgggg
     1081 caggtaaatc cactttgaca catcttattg caggtgttta tcagccaaca ataggtacta
     1141 taagtacaaa ccagcgtgat ttaaatatag gaatacttag tcaacagcca tatattttca
     1201 gtgcttctat aaaagagaat attacgatgt ttaaagatat agaaaataat actattgaag
     1261 aagtgctaga cgaagtaggt ttattagaca aagtgcaatc tttcacaaaa ggcattaaca
     1321 caataatagg tgaaggaggc gaaatgttat ctggtggaca gatgagacgc atagaacttt
     1381 gccgtctttt agttatgaag ccagatctcg ttatatttga tgagcctgca actggtttag
     1441 atattcaaac agaacacatg attcagaacg ttctgtttca acattttaaa gatacaacga
     1501 tgattgtcat tgcacataga gataatacaa ttcgccattt acaacgacgc ttgtatatag
     1561 aaaatggaag actgattgct gatgatcgca atatttcagt aaatataaca gaaaatggtg
     1621 atgacttatg aaaacacgac taaaatttca agtagataag gatttattgt tagctatagt
     1681 tgttggtgtt tgtggaagtt tagttgcgct cgccatgttt ttcttaagtg gttatatggt
     1741 gacacaaagt gcacttggtg cgccactata cgctctgatg attttagtcg ttacagtaaa
     1801 attgtttggg tttttaagag ctattactcg atacgtagag cgccttattt ctcataaagc
     1861 tacatttaca atgctacgtg atattcgggt acagtttttc ggtaaattag taaatgtcat
     1921 tcctaatgtt taccgtaaac tgagttctag tgatttaatt tcacgtatga ttagtcgtgt
     1981 tgaggcatta caaaatatat atttacgtgt ttattatcca ccagtcgtca tcggtttgac
     2041 agcgctagtt acagtcatag ttttggcgtt catttcaatc ggccatgcgc tattgattat
     2101 ggttagcatg ttgttcactt tactcattgt tccttggtta agctcaaaaa aagcacgtac
     2161 tttaaagaaa catgcagcta atgaacaggc ccgattttta aatcattttt atgattataa
     2221 agctggtatg gatgaactac gtcgatttaa tcaaattaat cattatcgag ataatttgat
     2281 ggctaaatta aatcattttg ataaattaca acttaaagag caacgctttt taacgattta
     2341 tgattttata ttaaatatta ttgctatgct ttcgattttt ggtagtttag ttctaggatt
     2401 aattcaaatt aatgcaggcc aactaaatat tatttatatg acgagtatag ttttaatggt
     2461 cttaacttta tttgaacaag ctgtaccaat gacaaatgtc gcgtattata aagcggatac
     2521 tgaccaagca ttgcacgata ttaatgaagt gatatctgta ccttctacta atggaaaaaa
     2581 acgtcttaat gataagtatg atgcaacgaa catttatgaa gttaaggatg ctagttttaa
     2641 gtattggaat cagcaaacgt atgtgttgtc ggatattaat tttaatgtta atagaggcga
     2701 aaagattgcg attgtgggtc cttctggttc aggaaaaagt acattactac aaattatggc
     2761 agggttatat caattagata gtggctctgt tcgtttcgaa aatatggata tgtttgaaat
     2821 agatgacaaa gataagtttg aatcgttaaa tgtcttgcta caatctcaac aattatttga
     2881 tggtacaata cgtcaaaatt tatttaccga tgaaaaagat gaagcggtgc aagcaatatt
     2941 taagcaatta gatttagaac atttggcact agaacgtcaa attgacttag atggtcatac
     3001 attatctggc ggagaaattc agcgtttagc gattacgagg atgttattaa aagatactgc
     3061 atcaacatgg attttagatg aaccaacaac tgcattagat aaacaaaata gtttaaaagt
     3121 tatggattta attgaagcac atgcagaaac attaattgtt gctacacacg atttaacttt
     3181 attgtcacgt tttgagacca tcattgtgat gataaatggt aaaatagttg aaaagggaaa
     3241 ctatcaacaa ttactagcta atcaaggtgc tttatggaat atgattcaat ataatgcata
     3301 aaaaaactgc ttgataggct aagaaacctt tcaagcagtt ttttaactaa caagcttacg
     3361 cgtgctaatt gtttttctat tcttaataaa ttctaaacat tactttaatt gtcatgacaa
     3421 aagttaatta tttttccttt gtttcatcaa atgcatgaat gactttacct aataagcgat
     3481 taagttcttt aacttcatct tgcgataaag aagaagctga agcgactttg tcagatgcat
     3541 tacttaattc tggtctaata gtttcacttt tgtcagtcaa gtgaataaat acttcacgtt
     3601 gatcgacttc ggaacgttca cgcttaatta agtctacttg ttccattcgt tttaataatg
     3661 gtgatactgt accagtatcg agtgctaatt cagttacgac tttcttgacg tttacaggag
     3721 attcatccca taaaattgtt aagacaagaa attgtgggta tgttagattg tacttcttaa
     3781 aaactttgtt agagtagtag cgattaactt gtctttgagc attgtacaaa ctaaagcata
     3841 gctgttcttt taaattatgt tgatcagaca ttaaagttct cctccagaca tactatccgt
     3901 ttttttctct tttcggattg gtaatcatta aaaagttgat tgtttattaa ttcacaactt
     3961 tctttgattc aatgccatgc taaaattaaa gtatgtttaa agtttagaag atatttttga
     4021 tta
//
LOCUS       DAKTLH010000180         3846 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.184, whole genome shotgun sequence.
ACCESSION   DAKTLH010000180 DAKTLH010000000
VERSION     DAKTLH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.184"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            238..522
                     /gene="groES"
                     /locus_tag="O0394_002504"
     CDS             238..522
                     /gene="groES"
                     /locus_tag="O0394_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCY0500557.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            598..2214
                     /gene="groL"
                     /locus_tag="O0394_002505"
     CDS             598..2214
                     /gene="groL"
                     /locus_tag="O0394_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCY0500558.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEEGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(2309..2855)
                     /locus_tag="O0394_002506"
                     /pseudo
     CDS             complement(2309..2855)
                     /locus_tag="O0394_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            2916..3128
                     /locus_tag="O0394_002507"
     CDS             2916..3128
                     /locus_tag="O0394_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island family protein"
                     /protein_id="HCY0500559.1"
                     /translation="MTDDNVNDHIIKNHIEMIVDRLATDKEFYIFDSLIQGLSYQDIS
                     SALDCSEQSVILWYETILDKIVGVIK"
     gene            3125..3715
                     /locus_tag="O0394_002508"
     CDS             3125..3715
                     /locus_tag="O0394_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCY0500560.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYNANSAHVTACRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGNVPIFINIGEWDGG
                     DEGLDKAVKDVSNDNPNHTVIVDDIPLEDYESIRFL"
BASE COUNT         1364 a          510 c          803 g         1169 t
ORIGIN      
        1 cggtaaaaat tgagacaata tataaataat aacagttagc aatgatgccc ataatcttgt
       61 cataattttc ctccaaatat ttgtttataa tttattttat cgtaaataac ttgaagttac
      121 aaaacttaat taaaaggtta tgacttgaaa ttttgaccaa atttgattat tataaatgta
      181 tgttagcact ctttaatgtt aagtgctaaa ctttaggttt tttaaggagg aacaatcatg
      241 ctaaaaccaa ttggaaatcg tgtgattatt gagaaaaaag aacaagaaca aacaactaaa
      301 agtggtattg ttttaactga tagtgctaaa gaaaaatcaa acgaaggcgt tatcgttgca
      361 gtaggaactg gacgcctatt aaatgatggt acaagagtga ctcctgaagt gaaagaaggg
      421 gaccgtgtcg tgttccaaca atatgctggt acagaagtta aacgagataa tgaaacatat
      481 ctggtattaa atgaagaaga tattttagca gttattgaat aatacagaac ttaattcata
      541 aataaattaa atagaacgaa aatgaaacac aactaaacaa atggaggttt atcatttatg
      601 gttaaacaat tgaaattctc tgaagatgca cgtcaagcaa tgttacgtgg tgttgaccaa
      661 cttgcaaatg cagttaaagt aacgattggt cctaaaggac gtaatgttgt attagataaa
      721 gagtttacag cacctttaat tacgaatgat ggtgtgacga ttgctaaaga aatcgaatta
      781 gaagatccat atgaaaatat gggggctaaa ctagttcaag aagtcgcaaa taagacaaat
      841 gaaattgctg gtgacggtac gacaactgca acagtattag ctcaagcaat gattcaagaa
      901 ggcttgaaaa atgttacaag tggtgcgaac ccagttggtt tacgacaagg tatcgacaaa
      961 gcagttaaag ttgctgttga agcgttacat gaaaattctc aaaaagttga aaataaaaat
     1021 gaaattgcgc aagtaggtgc gatttcagca gcagatgaag aaattggacg ttatatttct
     1081 gaagctatgg aaaaagtagg taacgatggt gtcattacaa ttgaagaatc aaatggacta
     1141 aacactgaac tagaagtggt tgaaggtatg caatttgatc gtggttatca atcaccgtat
     1201 atggttactg attcagataa aatggttgct gaattagaac gcccatacat tttagtaaca
     1261 gataagaaaa tctcgtcttt ccaagatatc ttacctttat tagaacaagt ggttcaatct
     1321 aatcgtccaa tcttaattgt agctgatgaa gttgaaggcg atgcattaac aaatatcgtg
     1381 ctaaaccgta tgcgtggcac atttacagct gttgcagtaa aagcacctgg ttttggtgat
     1441 cgtagaaaag cgatgcttga agatttagct attttaactg gtgcgcaagt gattactgat
     1501 gatttaggct tagatttaaa agatgcatca attgatatgt taggtactgc aagtaaagta
     1561 gaagtaacta aagataatac cactgttgtt gatggtgacg gtgacgaaaa cagcattgat
     1621 gcacgtgtta gccaattgaa atctcaaatt gaagaaactg aatctgactt tgatcgtgaa
     1681 aaattacaag agcgcttagc taaattagca ggtggtgttg cagttatcaa agtaggtgca
     1741 gcaagtgaaa cagagcttaa agaacgtaaa ttacgtattg aagatgcatt aaattctaca
     1801 cgtgcagcag ttgaagaagg tattgttgca ggtggtggta ctgcattagt aaatgtttac
     1861 caaaaagtaa gtgaaattga agctgaaggt gacattgaaa caggtgtaaa tattgtactt
     1921 aaagcattaa ctgcaccagt tcgtcaaatt gctgaaaatg caggattaga aggttctgtt
     1981 attgtagaac gtttgaaaaa cgcagagccg ggtgttggtt ttaacgctgc tacaaacgag
     2041 tgggttaata tgttagaaga aggtatcgtt gatccaacta aagtaacacg ctcagcatta
     2101 caacatgctg caagtgttgc agcaatgttc ttaacgactg aagcggttgt agcatcaatt
     2161 ccagaaaaaa ataatgacca acctaacatg ggtggcatgc caggaatgat gtaaaactgt
     2221 ttatagtttt gactaactag aaaattacat tatgcacgag tatgattcat tctaagtata
     2281 aattagaatg cacgcaaata tattttaatt acgagacgtc ttccatttgg tgactaaact
     2341 tatgggaggc gtcttttttg tatgaatttg tgattttagc atagatattc atagtggtat
     2401 tgatatcttt atgccgtaat cgttcttgta tttctttaat gtgtacacct gattctatga
     2461 gcaatgcgca atgtgtgtat ctgaatgagt gcgtagagat atttttgagt atgctggtac
     2521 gtgacataat ggtttgtatc cacattgata gttttttcat tacaatggtt atcattatat
     2581 aattcgtttt tccaattatt ttgaacgttt atcttataat cacgcaacac ctttattaca
     2641 tttgggtcaa cggaaatctt accgatagaa ctttcagttt tagggggaag tatctgatat
     2701 ttcttcttgt tattatttgg attgtaatat gttttagtaa tactaatcgt gttgttttca
     2761 aaatcaatat cagaccactt caatgctagt atctcgccag cacgacagcc ggtatatgca
     2821 agtgtgctaa agacttcaaa gctagttaac ggggcgatga tgaaataaat actgatgatt
     2881 tctttttaat aggtgatgaa gattctgacg aattgatgac agacgataat gtcaatgatc
     2941 atattataaa gaatcacata gaaatgattg ttgacagatt agcgaccgat aaagagtttt
     3001 atatttttga ctcccttata caaggactta gttatcaaga tattagtagt gccttagatt
     3061 gttcagaaca atctgtaata ttatggtatg aaaccatatt agataaaatt gtgggggtga
     3121 taaaatgagt gagttaactg caaagcaagc acgttttgtg aatgagtata ttagaacact
     3181 gaatgtaaca caaagtgcca taaaagcagg atataacgca aatagcgcac atgtgacagc
     3241 gtgtaggtta ttgaaaaagc cgcacatcaa gcaatatata caagaacaaa aagataagat
     3301 tatagatgag aatgtattaa ccgcaaaaga gttattacat gtgctaacga atgcggcagt
     3361 cggtgatgaa acagaaacga aagaagttgt agtcaagcgt ggagaatata aagaaaatcc
     3421 acaaagtggc aaagtacaat tagtctataa tgaacatgtt gaactgatag aggtaccaat
     3481 aaaacctagt gatcgtttaa aagctcgtga tatgttgggt aaatatcata aattatttac
     3541 agataagcat gatataaacg gtaatgtacc tatattcatt aatattggtg aatgggacgg
     3601 aggtgatgag gggttagata aggcagtgaa agatgtatct aacgataatc ctaatcatac
     3661 tgtgattgtg gatgatatac cgttagaaga ttatgagtca attaggtttt tataaattgg
     3721 gtatatattt tatggtgttg ccagtattta ttgatagtat cggttaagac gatgagaaga
     3781 atgagtgaga tttaaaagag ttgtaaacgc aatatcctaa tactgatttt catattgatg
     3841 atttag
//
LOCUS       DAKTLH010000181         3827 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.185, whole genome shotgun sequence.
ACCESSION   DAKTLH010000181 DAKTLH010000000
VERSION     DAKTLH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.185"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..160)
                     /locus_tag="O0394_002509"
     CDS             complement(<1..160)
                     /locus_tag="O0394_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCY0500561.1"
                     /translation="MLLNLLLIFILIVICPLIFNYTLTLNLTCYILVVISIFLSLIYS
                     IMTYTFPWS"
     gene            355..810
                     /locus_tag="O0394_002510"
     CDS             355..810
                     /locus_tag="O0394_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0500562.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            1050..2432
                     /locus_tag="O0394_002511"
     CDS             1050..2432
                     /locus_tag="O0394_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500563.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANISVTLKGDTKLNDSSK
                     KVTINDHEMAFSSSKTYGPYPQNKDITISASGKAKDKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            2463..2591
                     /locus_tag="O0394_002512"
     CDS             2463..2591
                     /locus_tag="O0394_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500564.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCGKIYEVLRFISFYTFYKL"
     gene            2701..>3827
                     /locus_tag="O0394_002513"
     CDS             2701..>3827
                     /locus_tag="O0394_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCY0500565.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIP"
BASE COUNT         1424 a          473 c          699 g         1231 t
ORIGIN      
        1 tagaccaagg gaaagtatag gtcatgatgc tatagatgag tgacaaaaat atcgatatga
       61 caactaagat gtagcatgtt aaatttaacg tcagagtata gttgaaaatt aacggacaaa
      121 taacgataag tataaatatt aataataaat tcaataacat actgacacct cgcttataat
      181 aaatattaaa tataaatgta gatgatttaa tttattaaag caaggagaaa gcagcaacat
      241 gtaaatctta atttgttata ttatatatgg gtcaatattt ttgtgttttt tagatgtgcg
      301 atacaatgat tgctgaggat actttataaa aatagtaatt agggagaaat aattatggta
      361 aaacatttac aagaccatat tcaattttta gagcagttta taaataacgt taacgcatta
      421 actgcaaaaa tgttgaaaga tttacaaaat gaatatgaaa tttcattaga gcagtctaac
      481 gtattaggta tgttaaataa agaacctttg acaattagtg aaatcacgca aagacaaggt
      541 gtaaataagg ccgcagtaag ccgacgaatt aaaaagttaa tcgatgctaa attagttaag
      601 ttagataaac caaatttaaa tattgatcaa cgtttgaaat tcataacctt aactgacaaa
      661 ggtagagcat atttgaaaga acgtaatgcg attatgacag atattgcgca agatattact
      721 aatgatttaa attctgaaga tattgaaaat gtaaggcaag tattagaagt catcaatcat
      781 cgcattaaaa catatagcaa tcataaatag gtttttgaag gtgttcaaat ataagactaa
      841 atatgtatgt attgaacacc ttctttttag ttataattga gagagtactt tcataaataa
      901 tgtaataagg tacaattcta aagtaaagtt aatcatttct tgaattgtat tcttgggata
      961 atatttgtaa aataataatg gtaaaataat gaaataattt ttgtgatata catataacaa
     1021 ttgaagagga acagaattga ggtgaaagta tgaaatcttg cccgaagtgc ggacaacaag
     1081 cacaagatga tgtacagata tgtacacaat gtggacataa atttgatagt cgtcaagcat
     1141 tatatagaaa gtcaaccgat gaagatatac aaactaataa catcaagatg agaaaaatgg
     1201 tgccttgggc aattggtttt ttcattttaa tcttaattat tatattgttc tttttactaa
     1261 gaaacttcaa ttcacctgaa gcgcaaacta aaatattagt gaatgcgatt gagaataatg
     1321 ataaacaaaa agtagcaaca ttattaagta ctaaagataa taaagtagat tctgaagaag
     1381 caaaagtata cattaactat atcaaagatg aagttgggct taagcaattt gtcagcgacc
     1441 ttaaaaatac ggtacataaa ttgaataaga gtaagaccag cgtagcttct tatattcaaa
     1501 ccagatctgg tcaaaatata ttacgtgtaa gtaaaaatgg cacacgttat atctttttcg
     1561 ataatatgag ctttactgca cctaccaagc aaccaattgt taaaccgaaa gaaaaaacaa
     1621 aatatgagtt taaatctggt ggtaagaaaa agatggttat agctgaagca aataaagtga
     1681 cgccaatagg taattttata ccggggacat atagaattcc agctatgaaa tcaactgaga
     1741 acggtgattt tgcaggccat ttaaaatttg attttagaca aagtaattct gaaacggtag
     1801 atgttactga agattttgaa gaagcaaata tatctgtaac tttaaaaggc gatacaaaat
     1861 taaatgatag ttctaaaaaa gtaactataa atgaccatga aatggcattt tcaagttcca
     1921 aaacgtatgg tccatatcca caaaataaag atattaccat ttcagcttca ggtaaagcga
     1981 aagataaaac atttacaaca cagacgaaga cgattaaagc tagcgattta aaatacaata
     2041 cagagataac tttgaatttt gacagtgaag atatcgaaga ctatgttgaa aagaaagaaa
     2101 aagaagaaaa cagcttgaag aacaaattga tagaattctt tgctggatat tctttagcga
     2161 ataatgctgc gtttaatcag tcggattttg attttgtatc atcatatata aaaaaaggat
     2221 catcttttta tgatgatgta aagaagcgtg tatctaaagg aagtttaatg atgattagtt
     2281 caccacaaat tatagatgct gaaaaacatg gtgataagat taccgcaacc gtaagattaa
     2341 taaatgaaaa cggtaagcaa gtagataaag aatatgagct tgaacaaggc tcgcaagacc
     2401 gcttgcaatt aatcaagaca tcagaaaaat agagtataaa acttaaataa gatgcatgtg
     2461 tcatggaata caaacaacat cctttcaaag tatttacaat tagtgtgaat tactttgaag
     2521 ggattgtttt ttgtggaaaa atttatgaag tgctgaggtt tatatcattt tatacctttt
     2581 ataagttgta atgatgttca tgaaacttgt gactaatgct aatatataaa agagcatatt
     2641 taaatttagt ttctgttcgt gatagttata aactaatcat tatataaagg agatagtcat
     2701 atgaatgatt taacgcattt taacaagcgt tcaccgatat ttattattat tttaggatct
     2761 ttgacggcca ttggagcatt atcgatagat atgtttttgc ctggattacc agatattaga
     2821 catgattttc aaacaacgac gtctaacgca caattaacat tatcgatgtt tatgattggt
     2881 ttagcgtttg gtaatttgtt tgctggtcca atttcagatt caactgggcg cagaaagccg
     2941 cttattatag ctatgattat ttttacatta gcaagtttag gtattgtttt tgtacataat
     3001 atttggctga tggttgcatt aagatttctt caaggagtga caggtggcgc agcagcagtt
     3061 atttcaagag ctatagcaag tgatatgtat agtggtaatg aactaactaa gtttatggca
     3121 ttattgatgc ttgttaatgg tattgcacca gttgttgcgc caacaatagg gggcataatt
     3181 ttaaattatt ctgtctggcg tatggtcttt gtcattctga ctatctttgg cttcgtcatg
     3241 gtcataggtt ctttattaaa agtacctgaa tcattaacag tgacaaatcg tgagtcaagt
     3301 agtggattaa agacaatgtt taaaaacttc aaaatattat tgaagacgcc acgttttgta
     3361 ttaccaatgc tcattcaagg tatgacattt gtaatattat ttacttatat ttctgcgtct
     3421 ccgtttatta ttcaaaaaat ttatggcatg actgcaatac aatttagttg gatgtttgca
     3481 ggaattggta ttacgctcat tatatcaagt caacttactg ggtaccttgt tgattttata
     3541 gactcacaaa aattgatgag aggcatgacg atgattcaga ttatcggtgt aattttagtg
     3601 acgattgtat tgcttaatca ttggaacttt tggattttag cgataggatt tatcatttta
     3661 atagcaccag tgacaggtgt tgcgacactt ggttttacaa tagctatgga tgaaagtagt
     3721 agtggtagag ggagttcatc aagtttatta ggtttagtac agtttttatt tggtggtgtt
     3781 gcatcgccat tagtgggggt aaaaggtgaa gataatccta tacctta
//
LOCUS       DAKTLH010000182         3638 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.186, whole genome shotgun sequence.
ACCESSION   DAKTLH010000182 DAKTLH010000000
VERSION     DAKTLH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.186"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(147..1874)
                     /locus_tag="O0394_002514"
     CDS             complement(147..1874)
                     /locus_tag="O0394_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0500566.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            2295..3590
                     /gene="pbp4"
                     /locus_tag="O0394_002515"
     CDS             2295..3590
                     /gene="pbp4"
                     /locus_tag="O0394_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HCY0500567.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQATNSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRTFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGTCLVAGLALIVHMIINRLFRKRK"
BASE COUNT         1276 a          638 c          551 g         1173 t
ORIGIN      
        1 aattattatg tcgtaacttc tcaaatgatt taaaatagac aaaagcttga agcaatttat
       61 taacatctat atccatcaat gtgccacact ttaatttcct cgatggtata tgtgtatttg
      121 ctccaagctt acttatatat ataaaattaa tctgttaatt tttgagacac tacaaagttt
      181 ttatacttcg catgtgatgc catcagttct gaatgcgtac ctttacctgt tacctgtcct
      241 ttgtctaaga atataatttg accggctttt ttaattgtag acaaacgatg cgcaatgaca
      301 atcgttgttc taccttccat caatgtttct aaagcttctt gaattttcaa ttcactttca
      361 ctatcgagat tagctgttgc ttcatcaagt aacaaaatat caggattttt aacaaaactt
      421 ctagcaatat caatacgttg acgttgtccg ccagacagtt tcaatcctcg ttcacctaca
      481 agcgtgtcat atccttcatc aaattgcatg ataaaatcat gacagttcgc taatttagca
      541 taattaataa gttcttcatc tgaaacatga cgattaattc cgtataaaat attgtctcta
      601 attgtaccac tcatcatcga atttgattgc ataacatatc caattttgcg tcgccactta
      661 gataacggga tatcatagac actttcaagg ccatatttaa tatcacctga ctcaatttca
      721 tacatacgtt ctatcagatt aaatatcgta cttttaccag acccagaagg gcctacaaaa
      781 gcactcactt gaccttgtgg gatttggaac gacacatcat ctaatatttt cttcacatca
      841 tatttaaagt ctacatgttc aaatgacaat acaccgtcat caattaatac attttcagaa
      901 tcttcaagag cttctgtcgg ttcaataggt tcttgcatga tttcgtatat tctactactt
      961 gcaccgactg cctttttata atctgtaact aacgtggaaa gattgattaa aggcatagat
     1021 aactgaataa cataaaatat cattgcaatt aatgtacctg cagtgattgc accagtcgca
     1081 atttctaatg caccaaaacc taaaataatt gcaattgtta gcaacataac tatacctgaa
     1141 attggttgta caactgccgc aattttagcc tgttttaaac ctaatttata tatttcattc
     1201 aaatttttat gtgcattatc taattcaaga cgctctgtat ttgatatttt aacaagacgc
     1261 atttcagtta ggacacgccc taacaaacca ctgaagtttg caatttcaga ttgtgtactt
     1321 gtcgatatct tttgcataat acgacctaga ggaatcataa ttaaaacgaa tatcggtatc
     1381 gttataaatg ttaataatgt cattttccaa tctaaaataa ataacatgat tagtgaccca
     1441 actaatgtaa cgattgatgg taataagtta ggtagctttt gtgaaataaa ttcatttatc
     1501 actttcgtat cgtcagttaa tcgactcatt aattgaccac tttcattttt gtcaaagaat
     1561 ggcattttta attgtatgat atgctcccat aaaactgagc gtatcgcata aataatcttt
     1621 tcaccaattt tacttaataa atataaacct aatccgctta ataaagcatt gatgacaaag
     1681 ataccaccaa ataatgcgat tagattccaa ttgatatggc tcacggaaaa tttatctaca
     1741 attcgtccag taaacagtgg cactaatagt ccacttaagc tccctagtga tgaaatagtg
     1801 atagctgcaa cgataagacc cactggccat gatagttttt taaataagaa aaacaatgga
     1861 ttttctcgtt tcatagtttt acctcttctg tttgaaattt atagttaatt catttagata
     1921 gatacatgca tttctatcat atactttctc ccaacaaaac tttaaaaatc aaataaagtg
     1981 ttgccccaaa ataccgctca taacatttat atgatagaat atttctattg cattttttgt
     2041 aataacttct caaagtcttt atattaaaac atgaaaatta atcgcttttg agtaagtttg
     2101 ctcttcgtac aaacattata acacctttag ctacacacgt aatatatttg cattattttt
     2161 caaattaact gcagaaaact ttattttcaa caatttaatt aaggtgaagt gaattagcaa
     2221 tgatttccgt aagtaggaat aaccattgct tttagggaat atttaagaat tggaaaaggg
     2281 aagattaacg ctttatgaaa aatttaatat ctattatcat cattttatgt ttaacattaa
     2341 gtattatgac accatatgca caagctacta acagtgacgt aacccctgta caagcagcaa
     2401 atcaatatgg ttatgcaggt ttgtcggctg catacgaacc gacgagtgct gttaatgtaa
     2461 gtcaaactgg acaattactg tatcaataca atatcgatac taagtggaat ccagcgtcta
     2521 tgactaaatt aatgacaatg tacttaacat tggaagctgt aaataagggg cagctttcac
     2581 ttgatgacac agtcacaatg acgaacaaag aatatattat gtctacacta cctgagttga
     2641 gtaatacgaa actatatcct ggacaagtat ggacaatcgc agacctatta caaattacag
     2701 tatctaattc tagtaatgcc gcggcattaa ttttagctaa gaaggtttca aaaaacacca
     2761 gcgatttcgt tgatttaatg aataacaaag ctaaagctat cggaatgaaa aatacacatt
     2821 tcgtcaatcc aacgggtgct gaaaattcaa gattacgtac atttgcacca acaaagtata
     2881 aagaccaaga acgtactgta acgactgcta gagactatgc cattttagat ttacacgtga
     2941 ttaaagagac acctaaaata ttagacttta caaagcagtt agcaccaaca acgcatgcag
     3001 ttacgtatta cacattcaac ttttcattgg aaggtgcaaa aatgagtttg ccgggtacag
     3061 atggtttaaa aactggatca agtgatacag caaattacaa ccatacgatt actaccaaac
     3121 gaggtaaatt tagaattaat caagttatca tgggtgcagg agactataaa aaccttggtg
     3181 gcgagaagca acgtaatatg atggggaatg cattaatgga acgctcattt gatcagtata
     3241 aatatgtaaa aatattgtct aaaggtgagc aaaggataaa tggtaagaaa tattatgttg
     3301 aaaatgatct ttacgatgtt ttaccaagtg attttagtaa aaaagattat aaacttgtag
     3361 tcgaagatgg taaagtacac gcggactatc caagagaatt tattaataaa gattatggac
     3421 ctccaactgt agaagttcat cagccaatta ttcaaaaggc aaatactgtt gctaaaagta
     3481 tgtgggaaga acatccatta ttcactatca ttggtggtac atgccttgtc gctggattag
     3541 cacttattgt tcatatgata atcaatcgtt tatttagaaa aagaaaataa aacatactaa
     3601 aaacggacaa gttgcacatt ataaagctgc gaaacttg
//
LOCUS       DAKTLH010000183         3607 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.187, whole genome shotgun sequence.
ACCESSION   DAKTLH010000183 DAKTLH010000000
VERSION     DAKTLH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.187"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(37..435)
                     /gene="tagD"
                     /locus_tag="O0394_002516"
     CDS             complement(37..435)
                     /gene="tagD"
                     /locus_tag="O0394_002516"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HCY0500568.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(498..1559)
                     /locus_tag="O0394_002517"
     CDS             complement(498..1559)
                     /locus_tag="O0394_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCY0500569.1"
                     /translation="MRFTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVIKPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHAILVKAMQKATDIHLVSQMIVGDNDIDTHATSNEE
                     DFNRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKF
                     LIQLKGRKFAHRTKSMLKR"
     gene            complement(1556..2659)
                     /locus_tag="O0394_002518"
     CDS             complement(1556..2659)
                     /locus_tag="O0394_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0500570.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLVLYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEDSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDQVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            complement(2758..3570)
                     /gene="tagG"
                     /locus_tag="O0394_002519"
     CDS             complement(2758..3570)
                     /gene="tagG"
                     /locus_tag="O0394_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HCY0500571.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
BASE COUNT         1142 a          649 c          499 g         1317 t
ORIGIN      
        1 ttaatttatc gtttagtatc gatagttcta tataatttat ttagcatctt taccatataa
       61 ttcttgtttg attttagtcg tcgaaatgcc ttctgtacgt tttaaataaa tgacttcaca
      121 tttatccttt aagaagtcga attcaccttc ccagtcatgt cccataacaa aaacatctac
      181 atcaaatttt tcgacatcgt cttctttttg tccccagccc ttttctggaa tgactaaatc
      241 gacatagcgt attgattcaa gcatcatttt tcgttgttca taatcataat aagatttttt
      301 atgtttaatt tgattaaatt catctgttga taatgctact attaaataat cgcccatctc
      361 tcttgcacga cgaagcaatt cgatatgacc atagtgaagt aagtcatatg tgccatatgt
      421 tattacacgt ttcattaagt tatcctcctc atattgtcac atcataggat tgcagtttat
      481 ggtcatcaat gtacacgtta tcgttttaac attgattttg ttcgatgtgc aaactttctc
      541 cctttaagtt gtatcaagaa ttttttataa gtcggcctat taatgcccac ttgaattaat
      601 gtttgtcgcc acaattcgta tatttctcta tcccaattct caccaataaa ttcaactgta
      661 tttataatgt caaacatgtt ttgcgataca taatcagaat gctgcataga cataatatag
      721 tcttttacga gttgaattgt cgtgtgcgtt actgctgggt gttcgttcat ataatatttt
      781 aatgaattat tgaataaaat acgatcaacc atatcactat ataatagcct ttgttcaggt
      841 aatagtaaca tttccatgac tcgttgtctt attttcataa tttctgtgat atatctatta
      901 aaatcttctt cgttacttgt agcatgtgta tctatatcgt tatctccgac aatcatctgt
      961 gaaactaaat gtatatccgt agctttttgc atcgccttga caagtattgc gtgattgtat
     1021 gtgttagcta aagtttcgtc acactgtaat tcagcaaatt tagcactgaa taactttccg
     1081 tcaaaagaca tcattgataa caaatctggt tgttccgtta atgtaacgat tcgattgtct
     1141 ttaataaact catcagcact tttcaatttc cattcaccat ggtaattgta agctcgctgc
     1201 cctattacca tatcaatatt atcattattt ttgaaagcag cagttaacct atctaattta
     1261 cctggcaata cgattgatgt tggttttatg accataaatg cgtcactatc atgtggatct
     1321 gtcattttga tatccatcaa acctttatta atacacgtag ctatcgaagc attttgtaat
     1381 tgtattaatg aaatatgctt gagttctctt tgtaaccgtt caatcatagg aattgtttga
     1441 tcagtagaac caccatcaat acaaaggatt ctatagtgtt ctttactctc aatagatatt
     1501 aacaattgtc gaattgttgc ctcattatta catgtaggta tgattatcgt aaacctcatt
     1561 ttgtcaccat cttatctata tattctgtga gctgatgtaa acttttatca gtattatact
     1621 tatgccaatc tttaaataac ggacttaata gatgttcttt ttcttgtatc gtcattatta
     1681 aatcttcttc agtatacact ttgtagctat ccggtattgc tttgtaaaat tgattcaggc
     1741 ctctcacctg atcatatgtt ccttcatcat acacataaaa tatagttgga atatctaaca
     1801 agctagcttc tattggcagc gaactatagt cgctaataat tatatctgac attagcatta
     1861 atgtagacgt gtcgattgaa gatacgtcat caatgtctga atcttcaatt gatggatgta
     1921 atttattaat cagtgtatat cctggtaaac atttttcaaa ataagcttta tcaatagccc
     1981 tattatctgc tttatcttct ctatatgttg gtacatataa taccaactta tttgtaattc
     2041 catatttatc ctttaactct gccttaaccg ttgctctatc agctgtgtaa tatttattaa
     2101 ttctcggaag cccaaaatac agcatttgct cttctgttgc acctaaagac tgtttaaaac
     2161 attgtgacat ttgttcacaa cccactaagt aaaaatccgt cgcttgataa actttacggt
     2221 actgctgaac cattgccttg tcagacacat cgacttgatg atctgttaag ccaaagtttt
     2281 ttaatgcacc acttgcatgc cacgtttgaa caatgtgttg attagaagtc ttattatatc
     2341 cacctagtaa taggtaataa ttatcgataa taatcatctg cgcgcttttc aaagccttaa
     2401 tttgttttac caatgttcga ttagtcattt ctatcacatc aacatcgtcg ctaagttcag
     2461 ataaataagg cgcttgtttt ggtgttgtta aaacagtttt ctgatacgac gaattattta
     2521 atgctttgat gataggctta atatcttctg gaaaagtcat cataaatacg atatgcggtt
     2581 tatcaatcac ttgaggcgta atcattttag aaagtattcg aactaccaaa tgataaaatt
     2641 tctttattaa aacgttcata atacaccaac ttaatatgtt atttacttaa attataaaca
     2701 aaaatgaacc ccacttccat ttattaatgg ttagcggggt ttcgtcatat aaatatatta
     2761 caagaagtct gcaaattgat ctctatattt catgtgtaag tacgcaccaa ttgcaaagaa
     2821 aatggcaaca ataccgaaat tgtataacat taatttccaa tgatccatga aataccattc
     2881 gtgatataaa attgctgcac ggtatgattc agcaataaag taaactggat tatatttcat
     2941 catttcatga attaaaccac tgataccatg gttctttggt agccacaaaa ttggtgaaaa
     3001 gtaaaataat attcttaata ttgcttgcat taacatttgt gtatctctaa ctaacacacc
     3061 gagtgttgat gttaataacg tcaccgaggc agttaagaaa aaacaaaacg gtacatatat
     3121 caataattga atgatatgta ttgatggata aataccagta aacatacatg caattatcac
     3181 aagtaaaagt aagcctaaat gtccataaaa tctacttgtc acaatatatg tcggtattat
     3241 cgataacggg aagttcattt tcgatacttg attaaacttt tgtgtaattg ctttagtacc
     3301 ttctaaaata ccttggttga tgaagaacca catactgata ccaaccaata accaataaac
     3361 aaaaggtaca ccatgaattg gtgcattact tcttattcct aatccaaaaa ccatccagta
     3421 aaccataatt tgcataacag ggttaattaa ttcccaagcc acacctaaat agttactatg
     3481 attgataatt ttaacttgaa actgagccag tctttgaatt aaataaaagt tctttacatg
     3541 ttctttaaaa actgttccta ttgctgacat tccattaaac cacactttca aatgtttaac
     3601 tatttct
//
LOCUS       DAKTLH010000184         3590 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.188, whole genome shotgun sequence.
ACCESSION   DAKTLH010000184 DAKTLH010000000
VERSION     DAKTLH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.188"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            85..1461
                     /locus_tag="O0394_002520"
     CDS             85..1461
                     /locus_tag="O0394_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCY0500572.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            1463..2602
                     /locus_tag="O0394_002521"
     CDS             1463..2602
                     /locus_tag="O0394_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCY0500573.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            2577..2942
                     /locus_tag="O0394_002522"
     CDS             2577..2942
                     /locus_tag="O0394_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HCY0500574.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WPKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQTRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            2945..3214
                     /locus_tag="O0394_002523"
     CDS             2945..3214
                     /locus_tag="O0394_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500575.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEHEIIAINDDGEIKIKISLSTKK"
     gene            complement(3415..3582)
                     /locus_tag="O0394_002524"
     CDS             complement(3415..3582)
                     /locus_tag="O0394_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HCY0500576.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
BASE COUNT         1276 a          527 c          679 g         1108 t
ORIGIN      
        1 gcaggtttac ctgataaaaa tacttattca ttatataatg ttaacaatat gtattttaaa
       61 gtttacattg agtgagggat attgatgaac gtaattttag aacagttgaa aacacatact
      121 caaaataaac ctaatgacat agcattacat atcgatgatg aaacaattac atatagtcaa
      181 ctaaatgccc gcatcactag cgcagttgaa tctttgcaga aatattcact taaccctgtc
      241 gttgctatta atatgaaatc accggtgcaa agtattattt gttatttagc tttgcatcgt
      301 ttacataaag tgcctatgat gatggaaggt aaatggcaaa gtactataca tcgtcaattg
      361 attgaaaaat atggtattaa agatgtaatt ggagatacag gtctcatgca gaatatagac
      421 tcaccgatgt ttattgattc aacgcaatta cagcactacc ccaatttatt acatattggt
      481 tttacttcag ggacaactgg actgccaaaa gcatattatc gtgatgaaga ttcatggttg
      541 gcttcttttg aagttaatga aatgttgatg ttaaaaaatg aaaatgcaat agcagcccct
      601 ggaccactat cgcactcgtt aacattatat gcgttattgt ttgctttaag ttccggtcgt
      661 acttttatag gacagaccac ttttcatcct gaaaagttac ttaatcaatg tcataaaata
      721 tcatcataca aagttgctat gtttcttgtt ccaacgatga ttaaatcatt attgttagtt
      781 tacaacaatg aacatacaat ccaatcattt tttagcagtg gagataagct gcattcttct
      841 atttttaaaa agataaaaaa tcaagcaaat gacataaatt tgattgaatt ttttggtaca
      901 tcggaaacca gttttatcag ctataacttg aatcagcaag caccagttga atcagtaggt
      961 gtgctatttc caaatgtgga attgaaaaca acgaatcacg atcacaatgg tataggaact
     1021 atttgtataa aaagtaatat gatgtttagt ggctatgtaa gtgaacaatg tataaataat
     1081 gatgaatggt ttgttactaa tgataatggc tatgtaaaag agcagtattt atatttaacg
     1141 ggacgtcaac aggatatgtt aattattggt ggtcaaaata tatatccagc acatgttgaa
     1201 cgccttttaa cgcaatcttc gagcattgat gaagcaatta tcatcggtat tccaaatgag
     1261 cgttttggtc aaataggcgt attgctttat tctggtgatg tgacacttac acataaaaat
     1321 gtaaaacaat ttttaaaaaa gaaagtgaaa cgctatgaaa ttccatcgat gattcatcat
     1381 gtagaaaaga tgtattacac tgcaagtggt aaaattgcta gagaaaaaat gatgtcgatg
     1441 tatttgagag gtgaattata atatgaatca agcagtcata gttgcagcta aacgaactgc
     1501 atttgggaaa tatggtggca ctttaaaaca tttagagcca gaacaattgc ttaaaccttt
     1561 attccaacat tttaaagaga agtatccaga ggtaatatct aaaatagatg atgtagtttt
     1621 aggtaatgtt gttgggaatg gtggcaatat tgcaagaaaa gcattgcttg aagcggggct
     1681 taaagattca atacctggcg tcacaatcga tcggcaatgt gggtctggac ttgaaagtgt
     1741 tcaatatgca tgtcgcatga tccaagccgg agctggcaag gtatatattg caggtggtgt
     1801 tgaaagtaca agtcgagcac cttggaaaat caaacgaccg cattctgtgt acgaaacagc
     1861 attacctgag ttttatgagc gtgcatcatt tgcacctgaa atgagcgacc catcaatgat
     1921 tcaaggtgct gaaaatgtgg ccaagatgta tgatgtttca agagaattac aagatgaatt
     1981 tgcttatcga agtcatcaat tgacagcgga aaatgtaaaa aatggaaata tttctcagga
     2041 aatattacct ataaccgtta aaggagaaat attcaacact gatgaaagtc taaaatcaca
     2101 tattccgaaa gataactttg gccgatttaa gcccgtgatc aaaggtggga ccgttaccgc
     2161 tgcgaatagt tgtatgaaaa atgatggtgc agttttattg cttattatgg aaaaagatat
     2221 ggcatacgaa ttaggtttcg agcatggttt attatttaaa gatggtgtta cggtaggtgt
     2281 tgattctaat tttcctggca ttggtccagt accagccatt tccaacttac taaaaagaaa
     2341 tcaattaacg atagaaaata ttgaagtcat tgaaattaac gaagcgttca gtgcacaggt
     2401 agttgcctgc caacaagctt taaatatttc aaatacgcaa ttaaatatat ggggtggtgc
     2461 attagcatca ggtcatccat acggtgcaag cggtgcccaa ttagtgactc gattatttta
     2521 tatgtttgac aaagagacta tgattgcatc tatggggata gggggaggtc taggaaatgc
     2581 agcattattt actcgattct aaccagcgat taaatgtgtc attttctaag gatagtgtgg
     2641 ctgcatatta tcagtgtttt aaccaacctt atagaaaaga agtaccacca ttaatgtgtg
     2701 cgtcattatg gccaaaattt gatttattta aaaaatatgc aaatagcgaa ctgattttaa
     2761 caaaatcagc aattaatcaa actcaaaaga tagaagtaga cacaatatat gtagggcatt
     2821 tagaagatat tgaatgccga cagactcgca atatcacacg ttatacaatg gctttaacat
     2881 taactaaaaa tgatcaacat gtcataacgg ttacacaaac ttttattaag gcgatgaagt
     2941 agagatgaag tttaatgaga tatggataaa tgaatatttg gcgctcgtaa atgatgataa
     3001 tccaatacat aatgagattg tgccaggaca attagtgagt caaatgatgc tgatggctat
     3061 gtcattagag acaaaccagt gtcaaattaa ctacgttaaa cctattttaa taaatgaaaa
     3121 tatcgaattc attgaacaac acgaacacga aattatagca attaatgacg atggagagat
     3181 taaaataaaa atttctttga gcacaaaaaa ataaccgata ttagctgcat gaacgcatat
     3241 taattaggag atgaaaggac agctaatatc agttatgtat tgttattatt attgggaaca
     3301 gagatgaata taggttacgt ttctttcttt gcacggggat gcattaatct aaaataataa
     3361 taacaactat atcaatgttt aataaattct ggattattgg aacgattagt caatttaact
     3421 aactttcata tgatctatat cgtcttgtaa taaagagagc aatttgaata tttcagtatc
     3481 actaaatgaa tcgtcacatt taattgaaac atgctgaaac gttttggtta taatttcata
     3541 aactggtgcg ccttcatggt gatactgtcg ataaataatc ataacctata
//
LOCUS       DAKTLH010000185         3574 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.189, whole genome shotgun sequence.
ACCESSION   DAKTLH010000185 DAKTLH010000000
VERSION     DAKTLH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.189"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..297
                     /locus_tag="O0394_002525"
     CDS             <1..297
                     /locus_tag="O0394_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCY0500577.1"
                     /translation="PQKVVTDQAPSTKAAMAKIIKAFKLKPDCHCTSKYLNNLIEQDH
                     RHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIMLAS"
     gene            355..732
                     /locus_tag="O0394_002526"
                     /pseudo
     CDS             355..732
                     /locus_tag="O0394_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            729..1610
                     /locus_tag="O0394_002527"
                     /pseudo
     CDS             729..1610
                     /locus_tag="O0394_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1726..2655
                     /locus_tag="O0394_002528"
     CDS             1726..2655
                     /locus_tag="O0394_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0500578.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGA
                     GKTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLT
                     INAKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILD
                     EPTNGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEI
                     MYEDLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINR
                     LLVLNNIDVFQMRVIEDNLETYFEDLVGGGTIG"
     gene            2648..3385
                     /locus_tag="O0394_002529"
     CDS             2648..3385
                     /locus_tag="O0394_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0500579.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKV
                     PTPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLKNLLIIPISRESFL
                     VSKIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSP
                     PIIILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFL
                     LSLIIIIVTSIFSVIILFSYFKKTDIS"
     gene            3399..>3574
                     /locus_tag="O0394_002530"
     CDS             3399..>3574
                     /locus_tag="O0394_002530"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="BacA; phosphatase activity in Escherichia coli not
                     kinase; involved in bacitracin resistance as bacitracin
                     supposedly sequesters undecaprenyl disphosphate which
                     reduces the pool of lipid carrier available to the cell;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase"
                     /protein_id="HCY0500580.1"
                     /translation="MGFIEQIKAFILGVVEGLTEFAPVSSTGHMIIVDDMWLNTADDL
                     GSASATTFKVVIQLG"
BASE COUNT         1308 a          491 c          632 g         1143 t
ORIGIN      
        1 cctcaaaagg tagttacaga tcaggcacct tcaacgaagg cagcaatggc taaaataatt
       61 aaagctttta aacttaaacc tgactgtcat tgtacatcga aatatctgaa taacctcatt
      121 gagcaagatc accgtcatat taaagtaaga aagacaagat atcaaagtat caatacggca
      181 aagaatactt taaaaggtat tgaatgtatt tacgctctat ataaaaagaa ccgtaggtct
      241 cttcagatct acggattttc cccatgccac gaaattagca tcatgctagc aagttaaacg
      301 aacactgaca tgatatatta gtgattagct atatttttta ctttgcaaca gaaccagcca
      361 tttaatatga ctgttacaat tactaagatt caagcactat tacgccgcac gtatgatttc
      421 accactcaag ttaataattt aacagtagat gattgcgagt tactacttga tgcagcaaag
      481 cttaaattta atgaagaaac catagacctg acacacactg aattgcaaat catgcatagc
      541 ttattccaga acaagggcaa attcgtcagt cgtaatgcgt taatcgaaaa atgctgggaa
      601 tcagaacact tcattgatga caatacgctt gctgtcaaca ttacacgttt acgcaaaaag
      661 ctacaaaaac taggacttac agattttatc gaaacgaaga aaaatgtcgg atatcgtgta
      721 ggtgacgtat gaatttcttg aaatcaatcc aacgtgaaat ttatattgtc atccttactt
      781 gtgctttatt ctactttata ttattcatgt ataatgtgcc gatgcaagca tatatgattg
      841 gtctaggtat tgtattgttt gtttcaatga tttactgggg tatccattac atgaacttta
      901 aaaaagaata aacactacag gaacagaacg aagatttaag aatcgaaaac cataaacttc
      961 gtaatgactt tattaactac caaagtgagt tagagtcata ttttttaatg tgggtgcatc
     1021 aaatcaagac accgattaca gcatcaaagt tactgcttga agatcctgat gaacacgtga
     1081 tttctaacgt gcgtaatgaa gtcttacaaa ttgataatta cacgaatctt gcactgaact
     1141 atttgaaact catgaaccaa aagacataca tggtctttat ggaagtgagt gtagatgaat
     1201 taattcgacc actgattaaa cgttatgcga tacagtttat tcatcatcaa acaaagttgc
     1261 attatgagaa aagtgaagcg gttatattaa cagatgccaa atgggcatca attatgattg
     1321 agcaaatatt aaacaatgca ttaaaatatg cacgtggcaa agatgtgtgg attacgtttg
     1381 acgaaaatac aaagaaatta tcaatccgag ataacggtat cggcattaat gcgagtgatt
     1441 taccgaaaat ctttgataaa ggtttctcgg gctataacgg tagaataagt gagaaatcaa
     1501 gcggtattgg tttatatatt gtcagcacaa tctcaaagcg cttaggccat aaagtaagtg
     1561 ctacatctac attaaacgat ggttcaatat tcaccattca atttaaataa ctccaatctt
     1621 tcatttttgt aagattagcg gctgtttttg taagaataga taaaggcagc cttttctaat
     1681 cgcgcgtaag ataaaggtaa caacaaacta agaaaggtgc cagaggtgaa aaatttgaat
     1741 aatataattg aaataaataa cttaacaaag caatatggaa gtcagatagc agttaataat
     1801 ttgagtataa ctgttgaaag tggacaaatt tatggttttt taggaagaaa tggtgcaggc
     1861 aaaacaacta ctataagaat gcttttaggc ttaattaaac ctacgaaagg taatattaaa
     1921 atatttgaaa aagagttcga aagtaataaa actgaaattt taaaaaatat tggtgctatg
     1981 gttgaaacgc caggttttta tagtaatttg acagctaaag agaacttaac tataaatgct
     2041 aaaatattag gtatacaaaa agaaaatgca attgaagaag tgttagaaat agtaggacta
     2101 gcttctgaaa aagtaaaatt gtttaaaaat tattctttag gtatgaagca acgtttaggg
     2161 attgcgagag ctttattgca caatcccgaa ttattgatac ttgatgagcc gacgaatgga
     2221 ttagatccta ttggcataaa agaaattaga aagttgatta aggatctggc tcaatctaga
     2281 aatataacca ttttaatttc aagtcatata ttgtcagaaa tagagcagtt agtagacaag
     2341 gtgggtatta ttcataacgg agttttgtta gaagaaatta tgtatgaaga tttaaaaaaa
     2401 aagaacagac aatttataag tatatcagta tcagatgaag aaaaagttgc atgtttgttg
     2461 gaaactaact tcggaattta tgactataaa attaaagaaa atggcgaatt taaaatttat
     2521 tcacatattg atagacaaca agaaattaat agattattag tattaaacaa tattgatgtt
     2581 ttccagatga gagtgataga agataatcta gaaacttatt tcgaagactt agtaggaggt
     2641 ggcacaattg gataatctac tccgtacaga atttattaag ttaaaaagat catatatgtt
     2701 tctaataagt attttaggta catgcgtagc accttttatg gtggttgtag cttcttttat
     2761 tagtatgcgg acgaaagtac cgactcctaa tattgtattt aaacagttat ttgaagaagt
     2821 cagtttatat catatgctaa ttattggacc tcctctatat ggggttataa tagcctattt
     2881 gtttggaaga gaatatatag aagatacttt aaaaaactta ttaattatac ctatatctag
     2941 agaaagtttt ttagtaagta aaataatatt attattcttg tggattcaaa cactatctgt
     3001 tattgcatgg ttgctaactt taatgctagg tatgctattg cattttcaag ggttaagcca
     3061 gagtttaata attgattcat tctttacatt tgttattggc ggagtgttat tatggatatt
     3121 atctccacca ataattattt tgtcgatata ttttaaaaat tatgttccca caattgtttt
     3181 aaccataata attactttga taaatttaat gactgctaat tctgaacata gaggattatt
     3241 tccttgggct gcagttggag acatatctag aggaacatta cctaatactt atccatttct
     3301 attatcttta attatcataa ttgttacatc tatattcagt gtaattatat tattctcata
     3361 ctttaaaaaa actgatataa gttaaaagga ggagaaaaat gggttttata gagcagatta
     3421 aagcatttat tttaggtgta gttgaagggt tgacagaatt tgctccagta tcatcaacag
     3481 gtcatatgat tattgttgat gatatgtggt taaacactgc tgacgattta ggaagtgcgt
     3541 ctgcaactac ttttaaggtg gtaatacagt tagg
//
LOCUS       DAKTLH010000186         3568 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.190, whole genome shotgun sequence.
ACCESSION   DAKTLH010000186 DAKTLH010000000
VERSION     DAKTLH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.190"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            7..2082
                     /gene="topA"
                     /locus_tag="O0394_002531"
     CDS             7..2082
                     /gene="topA"
                     /locus_tag="O0394_002531"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCY0500581.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKVEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFAPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            2238..3545
                     /gene="trmFO"
                     /locus_tag="O0394_002532"
     CDS             2238..3545
                     /gene="trmFO"
                     /locus_tag="O0394_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HCY0500582.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
BASE COUNT         1368 a          533 c          706 g          961 t
ORIGIN      
        1 ggggaaatga cattggcaga taatttagtc attgttgaat cgcctgcaaa agcaaaaacc
       61 attgaaaagt atttaggtaa gaaatataaa gttatagctt caatgggaca cgtcagagac
      121 ttaccaagaa gtcaaatggg tgtcgacact gaagataatt acgaaccaaa atatataaca
      181 atacgcggaa aaggtcctgt tgtaaaagaa ttgaaaaaac atgcaaaaaa agcgaaaaac
      241 gtctttctcg caagtgaccc cgaccgtgaa ggtgaagcaa ttgcttggca tttatcaaaa
      301 attttagagc ttgaagattc taaagaaaat cgcgttgttt tcaacgaaat aactaaagac
      361 gctgttaaag aaagttttaa aaatcctaga gaaattgaaa tgaacttagt cgatgcacaa
      421 caagcgcgtc gaatattaga tagattagtt ggctataaca tctcgccagt tctttggaaa
      481 aaagtaaaaa aagggttgtc agcgggtcga gttcaatctg ttgcacttcg tttagtcatt
      541 gaccgtgaaa atgaaattcg aaactttaaa ccagaagaat attggactat tgaaggagaa
      601 tttagataca aaaaatcaaa attcaatgct aaattccttc attataaaaa taaacctttt
      661 aaactaaaaa cgaaaaaaga tgttgagaaa attacagctg cattagatgg agatcaattc
      721 gaaattacaa acgtgactaa aaaagaaaaa acgcgtaatc cagcaaaccc atttacaact
      781 tctacattac aacaagaggc ggcacgtaaa ttaaacttta aagcaagaaa aacaatgatg
      841 gtcgcacaac aattatatga aggtatagat ttgaaaaaac aaggtacgat tggtttaata
      901 acatatatga gaaccgattc tacacgtatt tcagatactg ccaaagttga agcaaaacag
      961 tatataactg ataaatacgg tgaatcttac acttctaaac gtaaagcatc agggaaacaa
     1021 ggtgaccaag atgcccatga ggctattaga ccttcaagta ctatgcgtac gccagatgat
     1081 atgaagtcat ttttgacgaa agaccaatac cgattataca aattaatttg ggaacgattt
     1141 gttgctagtc aaatggctcc agcaatactt gatacagtct cattagacat aacacaaggt
     1201 gacattaaat ttagagcgaa tggtcaaaca atcaagttta aaggatttat gacactttat
     1261 gtagaaacta aagatgatag tgatagcgaa aaggaaaata aactgcctaa attagagcaa
     1321 ggtgataaag tcacagcaac tcaaattgaa ccagctcaac actatacaca accacctcca
     1381 agatatactg aggcgagatt agtaaaaaca ctagaagaat tgaaaattgg gcgaccatca
     1441 acttatgcac cgacaataga tacgattcaa aagcgtaact atgtcaaatt agaaagtaag
     1501 cgttttgctc ctactgagtt gggagaaata gttcatgaac aagtgaaaga atacttccca
     1561 gagattattg atgtggaatt cacagtgaat atggaaacgt tacttgataa gattgcagaa
     1621 ggcgacatta catggaggaa agtaatcgac ggtttcttta gtagctttaa acaagatgtt
     1681 gaacgtgctg aagaagagat ggaaaagatt gaaatcaaag atgagccagc cggtgaagac
     1741 tgtgaaattt gtggttctcc tatggttata aaaatgggac gctatggtaa gttcatggct
     1801 tgctcaaact tcccggattg tcgtaataca aaagcgatag ttaagtctat tggtgttaaa
     1861 tgtccaaaat gtaatgatgg tgacgtcgta gaaagaaaat ctaaaaagaa tcgtgtcttt
     1921 tatggatgtt cgaaatatcc tgaatgcgac tttatctctt gggataagcc gattggaaga
     1981 gattgtccaa aatgtaacca atatcttgtt gaaaataaaa aaggcaagac aacacaagta
     2041 atatgttcaa attgcgatta taaagaggca gcgcagaaat aatattttta tttcctagat
     2101 acattttaag attgttaaat agaatcatta gtgaatctta ttttaaagat agtaaaggat
     2161 taatctaaat aagtgcggat aatataaaca taacaacata attaaaagac ataaatgaca
     2221 ataaaaggag tatagaaatg actcaaactg taaatgtaat aggtgctggt cttgccggtt
     2281 cagaagcggc atatcaatta gctgaaagag gaattaaagt taatctaata gagatgagac
     2341 ctgttaaaca aacaccagcg caccatactg ataaatttgc ggaacttgta tgttccaatt
     2401 cattacgcgg aaatgcttta actaatggtg tgggtgtttt aaaagaagaa atgagaagat
     2461 tgaattctat aattattgaa gcggctgata aggcacgagt tccagctggt ggtgcattag
     2521 cagttgatag acacgatttt tcaggttata ttactgaaac acttaaaaat catgaaaata
     2581 tcacagttat taatgaagaa attaatgcca ttccagatgg atacacaatt atcgcaacag
     2641 gaccacttac tacagaaacc cttgcgcaag aaatagtgga cattactggt aaagatcaac
     2701 tttatttcta tgatgcggct gctccaatta ttgaaaaaga atctattgat atggataaag
     2761 tttacttaaa gtcccgttat gataaaggtg aagctgcata tttaaactgt cctatgactg
     2821 aggatgaatt taatcgcttt tatgatgcag tattagaagc tgaagttgcg cctgtaaatt
     2881 catttgaaaa agaaaaatat ttcgagggtt gtatgccttt tgaagtaatg gcagaacgcg
     2941 gacgcaagac attactattt ggaccaatga aaccagtagg attagaagat ccaaagactg
     3001 ggaaacgtcc ttatgcggtg gttcaattaa gacaagatga cgctgctggt acactctaca
     3061 atattgttgg cttccaaacg catttaaaat ggggagctca aaaagaagtc attaaattaa
     3121 ttccaggctt agaaaatgtt gatattgtta gatatggtgt gatgcataga aataccttca
     3181 ttaattcacc ggacgtatta aacgagaaat atgaattgat ttcacaacca aacatacagt
     3241 ttgcgggaca aatgactggt gttgaaggtt atgtagaaag cgcagctagc ggcttagttg
     3301 caggtatcaa tcttgcgcat aaaatattag gcaagggtga ggtagtattt ccgagagaaa
     3361 caatgattgg aagtatggct tactatattt ctcatgctaa aaacaataag aatttccaac
     3421 ctatgaatgc taacttcggg ttattaccat ctttagaaac tagaattaaa gataaaaaag
     3481 aacgctatga agcacaagct aatagagctt tggattactt agaaaatttc aaaaaaactt
     3541 tataaaatag ttagaaagac tagatatg
//
LOCUS       DAKTLH010000187         3567 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.191, whole genome shotgun sequence.
ACCESSION   DAKTLH010000187 DAKTLH010000000
VERSION     DAKTLH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.191"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..365
                     /locus_tag="O0394_002533"
     CDS             <1..365
                     /locus_tag="O0394_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="plasmid recombination enzyme"
                     /protein_id="HCY0500583.1"
                     /translation="KNKRLEDRLLDEQIKNGKVIDEYNDLADSYNNLLEQNQEKEKEL
                     NRSYKLFNNVFKLIKGVMKEETYHSLINHIDNHLESSKMRETMIVDDNDEQFFKKKYQ
                     RHEPEIIFEDERDDGYTL"
     gene            complement(536..859)
                     /gene="qacC"
                     /locus_tag="O0394_002534"
     CDS             complement(536..859)
                     /gene="qacC"
                     /locus_tag="O0394_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacC"
                     /protein_id="HCY0500584.1"
                     /translation="MPYIYLIISISTEVIGSAFLKSSEGFSKFIPSLGTIISFGICFY
                     FLSKTMQHLPLNITYATWAGLGLVLTTVVSIIIFKEQINLITIVSIVLIIVGVVSLNI
                     FGTSH"
     gene            1484..2422
                     /locus_tag="O0394_002535"
     CDS             1484..2422
                     /locus_tag="O0394_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011167206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCY0500585.1"
                     /translation="MIKKAEEIQAKQSLENENLNFSKTGYSNSRLSVQTLGQSQPKLS
                     FDAMTIVGNLNKNNAQKLSEFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADT
                     WDRRNMRVEFNPNKLTHDEMLWLKKNVIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDK
                     TVKKTVLYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVMSEHLWRVEVELK
                     RDMVDYWNDCFNDLHILKPAWTTLEKINEQAMVYTLLHEESMWGKLSRNTKYKFKNLI
                     KEISPVDLTELMKSTLKANEKQLQKQIDFWLSDFEF"
     gene            2664..>3567
                     /locus_tag="O0394_002536"
     CDS             2664..>3567
                     /locus_tag="O0394_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCY0500586.1"
                     /translation="MPYSIIRVAKVKSKTNTKGIQKHVQRQNKNYENMDIDLEKSYLN
                     YDLVNDSPIDYNQKIDEKIEQNYNGKRKIRKDAVKHIDGLITSGNEFFKNQTLEETKQ
                     FFEHAKTFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITEDGRLSAKEVLGNKKALT
                     EFQDRFNEHINSCGYDLSRGITRGVTPRRHEQISRYKNLTDYHKEEYEHESRKLDRIK
                     QESEEVMEQYQNALDVLKKPINVPYELETEKVGGLFNKETQETGNVVIDKNEFDLLQE
                     QVKASQLITDDYEYIKSGKALKDFE"
BASE COUNT         1396 a          435 c          650 g         1086 t
ORIGIN      
        1 aaaaaaacaa aagattagaa gatagattat tagacgaaca gattaaaaat ggaaaagtaa
       61 tagatgaata caatgattta gcggatagtt ataataattt attggaacaa aatcaagaaa
      121 aagaaaaaga gttaaaccgt tcgtataaac tatttaacaa tgtgtttaag ttgattaaag
      181 gtgtgatgaa agaagaaaca tatcattcgt tgattaacca tattgataat cacttagaaa
      241 gctcaaaaat gcgagaaaca atgattgtag atgataacga tgaacaattc tttaagaaaa
      301 aatatcaaag gcatgaacca gaaattattt ttgaagatga acgagatgat ggctacactt
      361 tataaagacg acgcatttat gccgagaaaa tttattgtgc gttgagaaga acccttaact
      421 aaacttgcag acgaaagtcg gcatagcgtg agctattaag ccgaccattc gacaagtttt
      481 gggtttgtta agggttcgga ggctcaacgt caataaagca attggaataa agcaattaat
      541 gcgatgttcc gaaaatgttt aacgaaacta cgccgactat gattaaaact atagatacta
      601 tagttattag atttatttgt tctttgaaaa taattattga gactacggtt gttaagacta
      661 aacctagtcc cgcccaagtt gcataagtta tatttagtgg taggtgttgc attgttttac
      721 ttaaaaaata gaaacaaatt ccaaatgaaa ttattgttcc taaggatggt ataaattttg
      781 aaaagccttc tgaagattta agaaatgcac ttccaataac ttcagtactt atggatatta
      841 ttaaataaat ataaggcatt tttaactcct tttaattttc gtattttatt taattttatt
      901 ctaagtattt ttcatttgca acgatccttt tattttttat ttgtatcagt atcaatttta
      961 cagtaaacat gcatttatgc cgagaaaatt tattgatgtt gagaagaacc cttaactaaa
     1021 cttggagacg aatgtcggca tagcgtgagc tattaagccg accattcgac aagttttggg
     1081 attgttaagg gttccgaggc tcaacgtcaa taaagcaatt ggaataaagc aataaaagga
     1141 gttgaagaaa tgaaatttag agaagctttt gagaatttta tagcaagtaa gtatgtactt
     1201 ggtgttttag tagttttaac tgtttaccag ataatacaaa tgcttaaata aaaaaagacc
     1261 tgatctgatt agaccaagtc ttttgatagt gttatattaa taacaaaata aaaagaagtc
     1321 gctcactccc tcgccaaagt ttgtgatagc gacttaccta gaaaaaacac taagttgttt
     1381 ttttatatct tgtatatcta gatattaaac gatataagtt tattcttcaa gatatatatt
     1441 cgggtgagcg acttcttaaa ttaaattaag gagttgtttt tttatgatta aaaaagcaga
     1501 agaaattcag gcaaaacaaa gcttagaaaa cgaaaactta aatttttcta aaaccggata
     1561 ctctaatagc cggttaagtg ttcaaacttt gggacaatct caaccaaaat taagttttga
     1621 tgctatgact attgttggaa atcttaataa aaataatgct caaaaactat ctgaatttat
     1681 gagtgttgag ccacaaatta gactttggga tatattacaa acaaaattta aagctaaagc
     1741 acttcaagaa aaagtttata ttgaatatga caaagtaaaa gcagatacat gggatagacg
     1801 taatatgcgg gttgaattta atccgaataa attaacacac gatgaaatgc tatggttaaa
     1861 gaaaaatgtt atcgactaca tggaagatga cggttttaca agattagatt tggcttttga
     1921 ttttgaagat gatttaagcg attactatgc aatgactgat aaaacagtta aaaaaactgt
     1981 tttatatggt cgtaatggca agccagaaac aaaatatttt ggagttcgtg atagtgatag
     2041 atttattaga atttataata aaaaacaaga acgtaaagat aacgcagatg ttgaagttat
     2101 gtctgaacat ctgtggcgtg tagaagttga attgaaaaga gatatggttg attactggaa
     2161 tgattgtttt aatgacttac acattttgaa acctgcgtgg actactttag aaaaaattaa
     2221 tgagcaagct atggtttata ctttgctgca tgaagaaagt atgtggggaa aattaagtag
     2281 gaatactaag tataaattta aaaatttaat taaagaaata tctccagttg atttaacgga
     2341 attaatgaaa tcgactttaa aagcaaacga aaaacaattg caaaagcaga ttgatttttg
     2401 gctatcagat tttgaatttt gagtgcatta aattttatac acgaagtgat cataaaattt
     2461 aaaaacgaat ttgcgaccac catttttggt cgcaggtttt gaacttatgg actgacttgt
     2521 tcagtccatt ttttgtgctt gcacaaaaac tagcctcgca gagcacacgg tttaacgact
     2581 taattacgaa gtaaataagt ctagtgtgtt atactttaat aaataaagct acgcaaaaac
     2641 atgtgcagtg gagggatttt aatatgccgt attctattat cagagttgct aaagtaaaat
     2701 caaaaacgaa tacaaaaggt attcaaaaac atgtacaaag acaaaataaa aattatgaaa
     2761 atatggatat tgatttagaa aaatcatact taaattatga tttagtcaat gatagtccga
     2821 ttgattataa tcaaaaaatc gatgagaaaa ttgaacaaaa ttataatggg aaacgcaaaa
     2881 ttagaaaaga cgcagtaaaa catattgatg gtttaattac atctggtaat gaatttttta
     2941 aaaatcaaac acttgaagaa acaaagcagt tttttgaaca tgctaaaacg tttttagaac
     3001 aagagtatgg aaaagataat ttattatatg caacagtgca tatggacgaa aaaacacctc
     3061 atatgcatta tggggtagtt ccgattacag aagatggtcg attgagtgcg aaagaggttt
     3121 taggtaataa aaaggcacta actgaatttc aagatagatt taacgaacat atcaatagtt
     3181 gtggttatga cttgagtaga ggtataacaa ggggcgttac accgagacga catgagcaga
     3241 taagtcgtta taaaaattta acagattatc acaaagaaga atatgagcat gagagccgaa
     3301 aattagaccg tataaagcaa gaaagcgaag aagtaatgga acagtatcaa aatgctttag
     3361 atgttctaaa aaagcctata aatgtcccat atgagcttga aactgaaaaa gttggtggac
     3421 tgttcaacaa agaaacacag gaaacaggta acgtcgttat tgataaaaat gaatttgatt
     3481 tattgcagga acaagtaaaa gcttctcaac tcattactga tgattatgaa tatattaaaa
     3541 gcggaaaggc tttaaaagat tttgagg
//
LOCUS       DAKTLH010000188         3557 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.192, whole genome shotgun sequence.
ACCESSION   DAKTLH010000188 DAKTLH010000000
VERSION     DAKTLH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.192"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(340..3354)
                     /gene="fnbA"
                     /locus_tag="O0394_002537"
     CDS             complement(340..3354)
                     /gene="fnbA"
                     /locus_tag="O0394_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HCY0500587.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATDNKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAP
                     QTAQPANIETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVE
                     VAQPRTASESKPRVTRSADVAEAKEASNAKVETGTDVTSKVTVEIGSIEGHNNTNKVE
                     PHAGQRAVLKYKLKFENGLHQGDYFDFTLSNNVNTHGVSTARKVPEIKNGSVVMATGE
                     VLEGGKIRYTFTNDIEDKVDVTAELEINLFIDPKTVQTNGNQTITSTLNEEQTSKELD
                     VKYKDGIGNYYANLNGSIETFNKANNRFSHVAFIKPNNGKTTSVTVTGTLMKGSNQNG
                     NQPKVRIFEYLGNNEDIAKSVYANTTDTSKFKEVTSNMSGNLNLQNNGSYSLNIENLD
                     KTYVVHYDGEYLNGTDEVDFRTQMVGHPEQLYKYYYDRGYTLTWDNGLVLYSNKANGN
                     GKNGPIIQNNKFEYKEDTIKETLTGQYDKNLVTTVEEEYDSSTLDIDYHTAIDGGGGY
                     VDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHHA
                     DVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTESNLIE
                     LVDELPEEHGQAQGPVEEITENNHHISHSGLGTENGHGNYDVIEEIEENSHVDIKSEL
                     GYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGNQSFEEDTEKD
                     KPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPSYQFGGHNSVDFEEDTLP
                     KVSGQNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEV
                     PAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPTKKPQSKKSELPETG
                     GEESTNKGMLFGGLFSILGLALLRRNKKNHKA"
     gene            complement(3338..3439)
                     /locus_tag="O0394_002538"
     CDS             complement(3338..3439)
                     /locus_tag="O0394_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500588.1"
                     /translation="MLLKTEQYRLAFIKFKKLMNFAFKGRYYSEKQS"
BASE COUNT          902 a          685 c          528 g         1442 t
ORIGIN      
        1 taacgtcact ataattttta tatcgttcgg tttttgtttg attttaatga ttatttatac
       61 aaaaacagcc gtatttcaag ccgacatttt aaatttaact aaatttgcat ctagttaata
      121 attgcattta tcaaatttgt cttattgatc caatctaatt tgtactcaca aactagttta
      181 aaattctaac tttatctctc agttcgttat caatcatcag acataaacca atgaagcaat
      241 cagaaaacac tctaattttc tattagaaat ttgatttaat ataaaaaaac aggcttactt
      301 catataattt atgaaataaa cccgtcaatt tttgtttaat tatgctttgt gattcttttt
      361 atttctgcgt aataatgcta aacctagaat gctgaataat ccgccgaaca acataccttt
      421 gtttgttgat tcttctccac ctgtttcagg tagttcagat ttcttagatt gtggtttttt
      481 agttggtgcc actgctttaa ccttttcatt gatttcaata acaggtgtta ctactttacc
      541 ttgttccact ggtttagaag gctttttagg ttcttctttg gcaggtggta ctggtttacc
      601 aggttcagct ggtacctctg gtgttggcgg tgttggagtt tctggctcac ttggtacttc
      661 tggtgttggt ggcgttggtg tttccggctc acttggtact tctggtgtcg gtggcgttgg
      721 tggcacgatt ggaggtgttg tatcttcttc aatcgtttgt tgaccttcat tttggccgct
      781 tacttttgga agtgtatctt cttcaaagtc aacactattg tgtccaccga attgataact
      841 tggtttatct ttatttgtat cttcttcaat aatttcagtg tgcttattga atccgtgaat
      901 atgtggcaca ctgtcgaagt cgatatcaat gatgttaccg ccatgttcat acttaggttt
      961 gtctttttct gtatcttcct cgaatgactg attaccttta ttttgaccat gaatttgagg
     1021 tacactatca aaatcgatat ctacgatatt gccaccttgt tcatatttag gtttgtcttc
     1081 ttctgtgtct tcctcgaatg actggttacc gctattttgg ccaccttcat aacctaattc
     1141 actcttaata tcaacgtggc tattttcttc gatttcttca atcacgtcat aattcccgtg
     1201 accattttca gttcctaaac cagaatgaga aatatgatga ttgttttcag taatttcctc
     1261 gactggtcct tgtgcttgac catgctcttc aggtaattca tccactaatt caatcagatt
     1321 actttcagtt gtatattctt tcgtatcttc aactgttgta tgatcgctca ctgcgccagt
     1381 tacaatacct tttgtagact cttcgtcaaa ttcaactaag ttagactcag tagtaacctg
     1441 accaccacct gggtttgtat cttcttcata ttcaacaaca tcagcgtgat gttttgaatt
     1501 ttcatgtgta gattcttcaa agtcaattgg atttgattcc tcagaggact cagtgtatcc
     1561 tccaacgtga cctgcttcgc tatccacagc agtatggtaa tcgatatcaa tagctgatga
     1621 atccgtttct tctattgttt caatgtatcc atcaacatat ccacctccac catctatagc
     1681 tgtgtggtaa tcaatgtcaa gagttgatga atcatattcc tcttcaacag tagttactaa
     1741 attcttatca tattgacctg taagagtttc tttaattgta tcttctttat attcaaattt
     1801 attattttga ataatcggac catttttccc atttccgttc gctttattac tgtataaaac
     1861 taaaccatta tcccaagtta aggtatatcc tctatcataa taatacttat aaagttgctc
     1921 tggatgtcct accatttgtg ttctaaaatc aacttcatca gtaccattta aatactctcc
     1981 atcatagtga acaacataag ttttatctag attttctata ttcaatgaat agcttccatt
     2041 attttgtaaa ttcaaattcc cactcatatt acttgtgact tctttaaatt tagaagtatc
     2101 tgtcgtattt gcatatacac tcttcgctat gtcttcatta ttacccaagt attcaaatat
     2161 cctaactttt ggttgatttc cattctgatt actacctttc attaaagttc cagtaacagt
     2221 cacacttgtc gttttaccat tattaggttt aataaatgca acatgcgaaa atctattatt
     2281 cgctttatta aatgtctcaa tcgatccatt taaattggca taataattcc caataccatc
     2341 tttatattta acatctaatt cctttgaagt ttgttcttca tttagtgttg aagttatagt
     2401 ttgatttcca ttagtttgta cagttttagg atcaataaat aaattaattt ctagttcagc
     2461 cgttacatca accttatctt caatatcatt tgtaaatgta tatctaatct ttccaccttc
     2521 taaaacttca cctgtcgcca ttacgactga accattttta atttctggta cttttctagc
     2581 agttgatacg ccatgcgtat ttacattatt tgataaagta aagtcaaagt agtcaccttg
     2641 atgtaaacca ttctcaaatt tcaacttata ttttagtacc gctcgttgtc ctgcatgagg
     2701 ttctacttta tttgtattgt tatgcccctc aatagaacca atttctactg taactttact
     2761 tgttacatct gtacccgttt ccactttcgc gttactagct tccttagctt ccgctacatc
     2821 tgctgatctt gtcacacgtg gcttactttc tgatgccgtt cttggctgtg ccacttcaac
     2881 ttgtgtttct gcgacttgat tttgtgtagc ctttttaggt gttaaatcta cttgtctttg
     2941 atctccgcta ttgtcttgag attgtgttgt ttccttaact tgaggtttcg cttcttcctt
     3001 aactacctct tctttaactg tttctatatt tgctggttgt gcagtttgtg gtgcttgtac
     3061 tgcttttggt gcttcttcag ttgttacttg tgttgcgttt gacggttgtt ctgttactgt
     3121 tgcgttatat gattgagttt cttctatatg attaacgtta gttgcagttg tttgtgtttc
     3181 acttgtttta ttatcagtag ctgaattccc attttcttct actgtagttg tcttttgttc
     3241 tgatgctgca gcttctttgt cttgtcccat cccaacaacg atcattgttc ctaagaatac
     3301 tgatgctgct cccaatttat gttttctaat gccgtaccta agattgtttt tcactataat
     3361 atctcccttt aaatgcaaaa ttcattaatt ttttaaactt aataaatgca agtctatatt
     3421 gttcggtttt taaaagcaat acaaaaaagc gagttaatga aaagttaaga ttgtcattaa
     3481 ttttatgtta attgataatt ttattatttg aaatatacct ataaattgta ttcaagtcat
     3541 cagaaaccct tgtcaca
//
LOCUS       DAKTLH010000189         3467 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.193, whole genome shotgun sequence.
ACCESSION   DAKTLH010000189 DAKTLH010000000
VERSION     DAKTLH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.193"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            175..519
                     /locus_tag="O0394_002539"
     CDS             175..519
                     /locus_tag="O0394_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0500589.1"
                     /translation="MNRNRMKQIILEYIKNNDSTSFVEIENVFEEQGFKYKGNGAYTS
                     GNHKNIIFWMGWNEEAFNIVADLKRDGLIEMQICPPMYYLIDGKGLRLPIVKNKNIKI
                     DHWLPVTFSLVN"
     gene            744..950
                     /locus_tag="O0394_002540"
     CDS             744..950
                     /locus_tag="O0394_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500590.1"
                     /translation="MLDHTNESVKRVFKYRDKQELLNKYHELLDKYFLHNISFYEEDK
                     NNERIYGLVVHTKNKLGDEDYERV"
     gene            937..1995
                     /locus_tag="O0394_002541"
     CDS             937..1995
                     /locus_tag="O0394_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HCY0500591.1"
                     /translation="MKEFKEQFGYQLSNFDDMDIKGYANLYQKDIGKDVSMIEQGLKQ
                     LSITETEVLLPEQINKKLLGVLNMNEVNQSSNTWVGTLTKQLVSSENNFNIQELPTAR
                     KEVFKELLVNCELPQSLREVITITDDEHVESIPSLTYIKDKLATNGIELSLNGSSKYF
                     DRREGHIYTEVSDSVVHGSDRTLDDLLKEIFINECVAYETTLLLDKNNSSGLIDKGNQ
                     DLSLYNQGIKEVSNTSIYDGIKQAMKDIPQTFRRNVSIVMNTEHHDKLIKELAQMGLG
                     GLAGDLSNLFNVTNVVVTDDAHDVFVGDFGHAIYAKYEPIMYNKKKQALKGIYQFALN
                     YVFDIKIIPELLRIVNIK"
     gene            2179..2670
                     /locus_tag="O0394_002542"
     CDS             2179..2670
                     /locus_tag="O0394_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0500592.1"
                     /translation="MVSDFESNDKITEVELLMHYNPKVINSKIKAMRSQIESLYHLNM
                     NHVITNDNDMLVSVSYPLDKLVLYIIEEKDKLEYYMKTAQDRLNLFKNIIKNYSENEQ
                     QDVMRYMLSSGKVKNEGVIERLKVDIYKVESRKRQERQNQREELHRIEFNKHLEQVKK
                     ELS"
     gene            2670..2822
                     /locus_tag="O0394_002543"
                     /pseudo
     CDS             2670..2822
                     /locus_tag="O0394_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            3099..>3467
                     /locus_tag="O0394_002544"
     CDS             3099..>3467
                     /locus_tag="O0394_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCY0500593.1"
                     /translation="MKPFESHNKQLKILRRRGMEVPSSAKRDLENENYYNIINGYKDL
                     FLELDVNGNFLVPDKYKQGTHFKEVFSLYKLDRKFRNVLLEYLLVFETHIKSRISYYF
                     SEKYREPHSYLYFKNYSSDTS"
BASE COUNT         1353 a          412 c          630 g         1072 t
ORIGIN      
        1 aggtaataaa agattatata aaataccgtg aattttcagt aactcagtaa ccaacagcct
       61 gtatgtattg acatcactgt gtttacagtg gttattgaaa aacataaatt cagtggtttt
      121 tagtatctaa cgtggttgct ttagtaactg gatagattgg aggactagag taacatgaat
      181 agaaatagaa tgaaacaaat catcttagaa tatataaaga acaatgacag cacttcattt
      241 gtagagattg agaatgtatt tgaagaacaa ggttttaaat acaaaggtaa cggcgcatat
      301 accagtggca accataagaa tattatattt tggatgggtt ggaatgaaga agcgtttaac
      361 attgtggcag atttaaaacg cgacgggcta attgaaatgc aaatttgtcc gccaatgtac
      421 tatcttattg atggtaaagg tctaaggcta ccaattgtaa agaataaaaa tataaaaata
      481 gatcattggc taccagtaac atttagttta gtaaattaaa acatgcagat aagcatttaa
      541 aatatcaagt atagatatgg gagtgtgtat atttattaat cataaaagaa tcgcatatca
      601 aataatcaaa catacacctg tgaacgttca attaatgaat acgaaatata taaatcgcgt
      661 tgtaaggcgc tatacaaaaa agcgtgctga ttttgaactt attaaaatgt atatctatca
      721 gattaaaagg agagaggcaa gacgtgctag atcatacaaa tgaatctgta aaacgtgtgt
      781 ttaagtatag agataagcaa gaactattaa ataagtatca cgaattatta gacaaatact
      841 ttcttcataa catttcgttc tatgaagaag ataaaaacaa cgaacgtata tatggtttag
      901 ttgttcatac taaaaacaaa ttaggagatg aagattatga aagagtttaa agaacaattt
      961 gggtatcaac tatctaactt tgatgatatg gatattaaag gatatgccaa tctatatcaa
     1021 aaggatatag gtaaagatgt aagtatgatt gaacaggggt taaagcaatt aagtattact
     1081 gaaacagaag ttttattacc agagcaaatt aacaaaaaat tattaggagt gttaaatatg
     1141 aatgaagtaa atcaatcaag taatacatgg gtaggtacct taacaaaaca attagttagt
     1201 agtgagaata actttaatat tcaagagtta ccaactgcac gtaaagaagt atttaaagag
     1261 ttattggtga attgtgagct acctcaatca ctacgtgagg tgattaccat tactgatgat
     1321 gaacatgttg aatctatacc atcactaaca tacattaaag ataagttagc tactaacggt
     1381 attgagctat cacttaatgg cagtagtaaa tactttgata gacgtgaggg acatatctac
     1441 acagaagtat cagacagtgt agttcatggc tctgatagaa cattagatga tctacttaaa
     1501 gagatattta ttaatgagtg tgtcgcatat gaaacaacat tgttattaga taagaataat
     1561 tcaagtggtt taattgataa aggtaaccaa gacttatcac tttataatca aggtatcaaa
     1621 gaagtaagta acacatcaat atacgatggt atcaagcaag cgatgaaaga tataccacaa
     1681 acgtttaggc gtaatgtatc tattgtgatg aacacagaac atcatgataa gctcatcaaa
     1741 gagttggcac agatgggatt aggtggactt gcaggtgatt taagtaacct atttaatgtg
     1801 actaatgtag tggttactga tgatgcacat gatgtctttg ttggagactt tggacatgcc
     1861 atatatgcta aatacgaacc tattatgtat aacaaaaaga aacaagcatt aaaaggtatt
     1921 tatcaatttg cattgaacta tgtgtttgat ataaaaataa ttccagagtt attgcgcata
     1981 gttaatatta aataaaaaaa ttatttaaat aaaattttat agccccctgc ccatcggctt
     2041 aaaatgtttt ttcgccgggt accggcgggg gcccttcgct tgcaacgcgg ataaactttt
     2101 atgaaagggg gataacttga tttatccctt tttcagatac ttataaatat taaattcgat
     2161 atgtaaaggt gtggttattt ggtatctgac tttgaaagca atgataaaat cactgaagta
     2221 gaacttttaa tgcattacaa tccgaaagtc attaatagca aaattaaagc gatgagatca
     2281 caaatagaaa gcttatatca cttgaacatg aaccatgtga tcacgaatga taacgatatg
     2341 ctggtatctg tatcatatcc attagataaa ttagtacttt atattataga agagaaagat
     2401 aaacttgaat attacatgaa aacagctcag gataggctaa atttgtttaa aaatatcatc
     2461 aaaaactatt ctgaaaatga acaacaggat gttatgaggt acatgttaag tagtgggaaa
     2521 gttaaaaatg aaggagtcat tgaacgttta aaagtagata tttataaagt tgaaagtaga
     2581 aagcgtcaag aacgacaaaa ccagcgagaa gaattacatc gaatagagtt taataagcat
     2641 ttagaacaag ttaaaaaaga actttcatag ataagcatga tattaacggg aatattccta
     2701 tattcattaa tattggtgaa tgggacggag atgatgagga attagataag gcagtgcaag
     2761 atgtatctaa cgctaaccat aatcatactg tgattgtgga tgatataccg ttagaggatt
     2821 gaagaaaatg aagctatgct atttataaat taatactaat tagtttgata ccatagctta
     2881 tttactgaga aagtagactt aaatgtaaca acaccagtgt ttattgataa tattggtggg
     2941 tttgaggagt agcaataaaa taaaggaggt aattgtgtaa aatatctctt tttgttattt
     3001 cttatttatt tacaaccgat aaaattaaat gtattatata tataacgatc tagccataac
     3061 tctattcggg ttatggctac ttttataggg gtaaatttat gaagccattt gaaagtcata
     3121 ataaacaatt gaaaattcta agaagaagag gaatggaagt accgagtagt gctaaaagag
     3181 atttagaaaa tgaaaattat tataatatca taaatggtta taaagattta tttttagaac
     3241 tagatgttaa tggtaatttt ttggttcctg ataaatataa gcaaggtact cattttaaag
     3301 aagtcttttc tttatacaaa ctagatagaa aatttaggaa tgttttatta gagtatttgt
     3361 tagtatttga aactcatatt aaatcaagaa tttcatatta ttttagcgaa aaatatagag
     3421 aaccacattc atatttatac tttaaaaatt attcatctga cacaagt
//
LOCUS       DAKTLH010000190         3399 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.194, whole genome shotgun sequence.
ACCESSION   DAKTLH010000190 DAKTLH010000000
VERSION     DAKTLH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.194"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            289..3132
                     /gene="sdrC"
                     /locus_tag="O0394_002545"
     CDS             289..3132
                     /gene="sdrC"
                     /locus_tag="O0394_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrC"
                     /protein_id="HCY0500594.1"
                     /translation="MNNKKTATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNETTAPSENKTTKKVDSRQLKDNTQTATADQPKVTMSDSA
                     TVKETSSNMQSPQNATANQSTTKTSNVTTNDKSSTTYSNETDKSNLTQAKDVSTTPKT
                     TTIKPRTLNRMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHVNQTTGKTEFWATSSD
                     VLKLKANYTIDDSVKEGDIFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDST
                     TNTTTYTFTNYVDQYTNVRGSFEQVAFAKRKNATTDKTAYKMEVTLGNDTYSEEIIVD
                     YGNKKAQPLISSTNYINNEDLSRNMTAYVNQPKNTYTKQTFVTNLTGYKFNPNAKNFK
                     IYEVTDQNQFVDSFTPDTSKLKDVTDQFDVIYSNDNKTATVDLMKGQTSSNKQYIIQQ
                     VAYPDNSSTDNGKIDYTLDTDKTKYSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTN
                     KDGKQDANEKGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGY
                     TPTTANVGTDDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKGVKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDNDSDSDSDSDSDAGKHTPAKPMSTVKDQHKTAKALPETG
                     SENNNSNNGTLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1387 a          520 c          581 g          911 t
ORIGIN      
        1 ttaattaaaa taagatgtaa tagcgaattg aaaacaagct tagattaaaa gtgagtttta
       61 aaaatataga taaataattt aaagcaatta aagaaaaaaa gtattaaaaa ttgcaattct
      121 taaactgtga ttcatattaa tatttctagc aaataacatt gtaaaataaa gaaaaataat
      181 taaagtattg cactttattg aaatttatat tacgatagta atgcagaaat ttatatatgc
      241 aaaatattat atttatcaaa ttttgatatt ttaaaggagt attattaaat gaataataaa
      301 aagacagcaa caaatagaaa aggcatgata ccaaatcgat taaacaaatt ttcgataaga
      361 aagtattctg taggtactgc ttcaatttta gtagggacaa cattgatttt tgggttaagt
      421 ggtcatgaag ctaaagcggc agaacatacg aatggagaat taaatcaatc aaaaaatgaa
      481 acgacagccc caagtgagaa taaaacaact aaaaaagttg atagtcgtca actaaaagac
      541 aatacgcaaa ctgcaactgc agatcagcct aaagtgacaa tgagtgatag tgcaacagtt
      601 aaagaaacta gtagtaacat gcaatcacca caaaacgcta cagctaatca atctactaca
      661 aaaactagca atgtaacaac aaatgataaa tcatcaacta catatagtaa tgaaactgat
      721 aaaagtaatt taacacaagc aaaagatgtt tcaactacac ctaaaacaac gactattaaa
      781 ccaagaactt taaatcgcat ggcagtgaat actgttgcag ctccacaaca aggaacaaat
      841 gttaatgata aagtacattt ttcaaatatt gacattgcga ttgataaagg acatgttaat
      901 cagactactg gtaaaactga attttgggca acttcaagtg atgttttaaa attaaaagca
      961 aattacacaa tcgatgattc tgttaaagag ggcgatatat ttacttttaa atatggtcaa
     1021 tatttccgtc caggatcagt aagattacct tcacaaactc aaaatttata taatgcccaa
     1081 ggtaatatta ttgcaaaagg tatttatgat agtacaacaa acacaacaac atatactttt
     1141 acgaactatg tagatcaata tacaaatgtt agaggtagct ttgaacaagt tgcatttgcg
     1201 aaacgtaaaa atgcaacaac tgataaaaca gcttataaaa tggaagtaac tttaggtaat
     1261 gatacatata gcgaagaaat cattgtcgat tatggtaata aaaaagcaca accgcttatt
     1321 tcaagtacaa actatattaa caatgaagat ttatcgcgta atatgactgc atatgtaaat
     1381 caacctaaaa atacatatac taaacaaacg tttgttacta atttaactgg atataaattt
     1441 aatccaaatg caaaaaactt caaaatttac gaagtgacag atcaaaatca atttgtggat
     1501 agtttcaccc ctgatacttc aaaacttaaa gatgttactg atcaattcga tgttatttat
     1561 agtaatgata ataaaacagc tacagtcgat ttaatgaaag gccaaacaag cagcaataaa
     1621 caatacatca ttcaacaagt tgcttatcca gataatagtt caacagataa tggaaaaatt
     1681 gattatactt tagacactga caaaactaaa tatagttggt caaatagtta ttcaaatgtg
     1741 aatggctcat caactgctaa tggcgaccaa aagaaatata atctaggtga ctatgtatgg
     1801 gaagatacaa ataaagatgg taaacaagat gccaatgaaa aagggattaa aggtgtttat
     1861 gtcattctta aagatagtaa cggtaaagaa ttagatcgta cgacaacaga tgaaaatggt
     1921 aaatatcagt tcactggttt aagcaatgga acttatagtg tagagttttc aacaccagcc
     1981 ggttatacac cgacaactgc aaatgtaggt acagatgatg ctgtagattc tgatggacta
     2041 actacaacag gtgtcattaa agacgctgac aacatgacat tagatagtgg attctacaaa
     2101 acaccaaaat atagtttagg tgattatgtt tggtacgaca gtaataaaga tggtaaacaa
     2161 gattcgactg aaaaaggaat taaaggtgtt aaagttactt tgcaaaacga aaaaggcgaa
     2221 gtaattggta caactgaaac agatgaaaat ggtaaatacc gctttgataa tttagatagt
     2281 ggtaaataca aagttatctt tgaaaaacct gctggcttaa ctcaaacagg tacaaataca
     2341 actgaagatg ataaagatgc cgatggtggc gaagttgatg taacaattac ggatcatgat
     2401 gatttcacac ttgataatgg ctactacgaa gaagaaacat cagatagcga ctcagattct
     2461 gacagcgatt cagactcaga tagcgactca gattcagata gcgactcaga ttcagacagc
     2521 gattcagaca gcgactcaga ctcagatagc gattcagatt cagacagcga ctcagactca
     2581 gacagcgatt cagactcgga tagcgactca gactcagata gcgactcaga ttcggatagc
     2641 gactcagact cagatagcga ttcagattca gatagcgatt cggactcaga cagtgattca
     2701 gattcagact cagatagcga ctcagattct gacagcgatt cagactcaga cagcgactca
     2761 gactcagaca gtgattcaga ttcagacagc gactcagatt cagatagcga ctcagactca
     2821 gatagcgact cagactcaga tagcgactca gactcggata gcgattcaga ttcagacagc
     2881 gactcagatt cagatagcga ttcggactca gacaacgact cagattcaga tagcgattca
     2941 gattcagatg caggtaaaca tactccggct aaaccaatga gtacggttaa agatcagcat
     3001 aaaacagcta aagcattacc agaaacaggt agtgaaaata ataattcaaa taatggcaca
     3061 ttattcggtg gattattcgc ggcattagga tcattattgt tattcggtcg tcgtaaaaaa
     3121 caaaataaat aatatgatta acttaaccag gtccatgtgg cctggttttt tctgtttaga
     3181 aattcccaga tataaaacaa aaaatcttta aggaatagat acaatttaat ttgttacaga
     3241 aatgtaattg tctatgaatt agaaaagcta agatattaat ttcaagggca aataatacta
     3301 ttttttatga tgattaaata aagattacaa aactcataaa aacttttatt taaacacaaa
     3361 tgttaaataa cgttttacga taaagaaaaa taattaaag
//
LOCUS       DAKTLH010000191         3385 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.195, whole genome shotgun sequence.
ACCESSION   DAKTLH010000191 DAKTLH010000000
VERSION     DAKTLH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.195"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            140..775
                     /locus_tag="O0394_002546"
                     /pseudo
     CDS             140..775
                     /locus_tag="O0394_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            772..1302
                     /gene="sat4"
                     /locus_tag="O0394_002547"
     CDS             772..1302
                     /gene="sat4"
                     /locus_tag="O0394_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HCY0500595.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFEKAVFWYLRF"
     gene            1395..2189
                     /locus_tag="O0394_002548"
                     /note="aph(3')-IIIa"
     CDS             1395..2189
                     /locus_tag="O0394_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCY0500596.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2465..3036
                     /locus_tag="O0394_002549"
                     /pseudo
     CDS             2465..3036
                     /locus_tag="O0394_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            complement(3205..>3385)
                     /locus_tag="O0394_002550"
     CDS             complement(3205..>3385)
                     /locus_tag="O0394_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCY0500597.1"
                     /translation="IEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQI
                     YGFSPCHEISIMLAS"
BASE COUNT         1122 a          537 c          762 g          964 t
ORIGIN      
        1 cgaacactga catgatatat tagtgattag ctatattttt tactttgcaa cagaaccatc
       61 atttttatta aaatactctt ttgtaaaatc tttctgctct tcaatgtatt taatttcttt
      121 acctctaaat ctattatagc tatttgatga ttacaataaa attgatctta ccttattgcc
      181 cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc taattgataa
      241 agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg taagaaagcc
      301 aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac cttatgttat
      361 taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc agattgttcg
      421 ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct ttaaatgaag
      481 tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt gggagaaact
      541 tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat ttctatgcca
      601 tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg cctatccgga
      661 gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca ctggtaaaac
      721 cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac agtgattaca
      781 gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt tgaggtgata
      841 ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt actctatgaa
      901 gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga ggcagattgc
      961 cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga cgataaatgc
     1021 gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga agatatcgcc
     1081 gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat atctatagaa
     1141 tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa taaccttata
     1201 gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac tatgttatac
     1261 gccaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa
     1321 ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag
     1381 gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
     1441 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
     1501 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
     1561 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
     1621 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg
     1681 tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg
     1741 cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct
     1801 tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt
     1861 gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa
     1921 agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca
     1981 tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg
     2041 acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac
     2101 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
     2161 attatatttt actggatgaa ttgttttagt acctagattt agatgtctaa aaagctttaa
     2221 ctacaagctt tttagacatc taatcttttc tgaagtacat ccgcaactgt ccatactctg
     2281 atgttttata tcttttctaa aagttcgcta gatagagttc tatattagag atgtaaagag
     2341 ttttggtgaa gaggtttgtg agtatggctt ttataaccag ggtgcaatga gaagcactaa
     2401 cagcagctag cttaagaacg cataggatat actttttatg gaagtcacag aggaggaata
     2461 aaatttgaac aggattaaca gagtaacctc tattcttatt cagctgcaat caaaaaaata
     2521 attcctgcca aagaaattgc acagcgattt aatataagct taaggacagt ttacagagat
     2581 atccggacac ttgaagaagc cggaatacca attggatctg aggccggaaa aggatatttt
     2641 cttgtagagg gcttcctact tctgccggta atgtttacag cggcggaagt gggtgcattg
     2701 attacagcag ggaaattttt aaattgccat ggagatgaat catttataaa ggattttgat
     2761 tcagctatgt ataaaatcaa atctattttg aagcatggtg aaaaaaacta tgcacaggag
     2821 ttggagaaca gtattaacgt gtacagcaca tctggacaga aaaatacatt ggcggataac
     2881 gttattgcag caattcagac tgcaatctgc aataaaaggg taatatcaat tcaatatcct
     2941 gcatcaggag ggcaagaacc ggaaagcaga atgatagagc ctatatcact ggggttttat
     3001 gaacagaact ggtacttaat cggttttgca ggctaagaaa tgaatatagg aattttcgtg
     3061 tggatcggat taaaagcatt tgcatagaag aagagctctg tcaaagccat gatggctctt
     3121 tggaacaaat attaaaacaa atgctggttc tgttgcaaag taaaaaaata tagctaacca
     3181 ctaatttatc atgtcagtgt tcgcttaact tgctagcatg atgctaattt cgtggcatgg
     3241 cgaaaatccg tagatctgaa gagacctgcg gttcttttta tatagagcgt aaatacactc
     3301 aatacctttt aaagtattct ttgctgtatt gatactttga taccttgtct ttcttacttt
     3361 aatatgacgg tgatcttgct caatg
//
LOCUS       DAKTLH010000192         3366 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.196, whole genome shotgun sequence.
ACCESSION   DAKTLH010000192 DAKTLH010000000
VERSION     DAKTLH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.196"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(136..2136)
                     /locus_tag="O0394_002551"
     CDS             complement(136..2136)
                     /locus_tag="O0394_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0500598.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFVIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDSVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTITKLITNDYDLSFSDNSKSQ
                     IERQQTIENIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGAIFSRQSVMITAFTAQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKNSTISINFNVFGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMIG
                     INNHHIFLTSYVLAVIIISFLYMAMYKITSYIYAKVIHQQRN"
     gene            complement(2133..2888)
                     /locus_tag="O0394_002552"
     CDS             complement(2133..2888)
                     /locus_tag="O0394_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0500599.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLAVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            complement(2996..>3366)
                     /gene="nsaS"
                     /locus_tag="O0394_002553"
     CDS             complement(2996..>3366)
                     /gene="nsaS"
                     /locus_tag="O0394_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HCY0500600.1"
                     /translation="KTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEF
                     DEQSNQLHVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHP
                     VSVVSKQNEGTTFTIQFPDE"
BASE COUNT         1109 a          587 c          481 g         1189 t
ORIGIN      
        1 tattaaaaac cgacaagaca ttttatctta tcggttgaaa tttgttattg ttatttgtaa
       61 tgtttttagg tttcttttta atataatata tttcagtgaa aatacatgat tgattgtgat
      121 ttcactgaaa catggttaat tgcgttgttg atgaataact ttagcataaa tataggaagt
      181 tattttgtac atcgccatat atagaaacga aattataatg acagctagta cgtaacttgt
      241 taaaaatata tgatggttat taatacctat catatttagt aacgtatata caatgttact
      301 agaaattaat gtgtgaatca gtgctactgt tattggtatt gcgaacaaga aagtcatttg
      361 atttcgtgtt atctttgcta ttcttccatt atctaaacca agttttttca taatgtcata
      421 attgtgctga tttgcatagg cttctgagac ttgtttataa tacatcatta gaaatattcc
      481 aatgacaagt acaattgata ctacgccacc tacaaatatt agtccacctg tcaaacgatc
      541 ccaaaccatt agcatttctg atttagaatt tattgacgca tcgtattttt tcgatagctt
      601 ttgtatttgt gatgttgtta atttcgtctt tccaaataca ttaaagttta tcgatattgt
      661 cgaattcttt tgttcatgtg cataaaatcc ttttactttt tgtaattgat tgttatcttt
      721 aacaattaaa accatactat cttgaatcat catcaaattt aacgcatgtg cgtcaagttg
      781 tttcacttta aagacttcat tattaagacc gaccttagac tgctttttaa aaataggtac
      841 attcgtaatc atacctatac tttgattgtc taagtgtaat ttgttttggt gatatcgatt
      901 ataatcttgt gcagtaaaag ctgtaatcat tactgattgt cttgaaaata tcgcgccttc
      961 attaccaatg aagtctattg gaatatcatc actagcctgt tttaacttgt aataagcacg
     1021 attcttttct aaagaagctc taaataatgt cgtttcatat actttgaagt cttttgcatt
     1081 aaccgaaccc tgtatatcac gtttaatatt ctcaattgtt tgttgacgtt ctatttgtga
     1141 cttagaattg tcgctaaatg acaaatcata atcattcgta atcaatttcg taatggtatg
     1201 attcatatca cgataggttg ttaatgtcat tgtgagcgtt acaatcaaaa atgtacacag
     1261 caaagtgatt gttgcaagac tgactgcatt cattttaaga cgtacacgca tcccaactac
     1321 cacaaaaaaa taccgtggat gatagtaaac ttttgatacc tgctgcaata tactaataat
     1381 tatttcactt atacctacaa aaaatgcata agtaccgata ataactaatc ctatgacaat
     1441 ccatatttta aaaaaggcac caaacgtcgt atcttgttgt aatgcaataa agtaacctaa
     1501 atatagtgct gcgcttccta taacaattaa aacgtaacgc aaccatcgtg atatgacact
     1561 agaatcggaa cgatgttgat aagttatcgg ccgttgaaaa ttaatactaa acaagttgaa
     1621 tataagtagc atgcccatta atacagcaat gataatcaaa gtaataaaca tcgctacaga
     1681 gtcaaatgga taatacctta acgtcgcaac ttcactgccc attattttct gtataaacaa
     1741 gaaaaatatc gcaccaagta agtatccgcc ggcaatacta acgactgaaa ttatcacaaa
     1801 ttgtaagata gtttccatca ctacaattaa acgcatactt ttcttggtca tgcccaatgt
     1861 cataaaaatg ctaaactctt ttcgtctttg tgacatcata aagtgatttg catagaaaat
     1921 aaaaataaaa gttaaaagcg ccataaaaaa attagctatg ataataaatg gtactaggaa
     1981 gtcattcttc tgttttatgt agctatttaa cccaattgac acaagcgtat attcaatcat
     2041 gaatagtaca cttaccgcta aaatgaatgg aatgattaca agtcgctgcg taataaattg
     2101 acgtcgcact atgtgccata acaatcttaa cttcatatat tgacacttcc tccattcaca
     2161 agtgctaagc tatctgttat tcgttgttga aaagctaatt gactttcttc accacgatat
     2221 atttcatgat atagacgccc atctttaata aaaatgactc gctctgcata agacgcatcg
     2281 atatttgaat gagttaccat taaaattgtc tgttccaatt gatgaatctc ttgaaataac
     2341 atcatcaatg cctttgatgt ttttgaatca agtgcacctg taggttcatc ggctagtaaa
     2401 atcgtcggct tagtaactaa tgcacgtgca atggcaatgc gttgcttctg acccccagag
     2461 atttcagaag gatacttgtt taagaatccc tctaaatgta attgtactgc caactgatgt
     2521 accctttgtt ctatatcttt tcgtttagca ccagctaaaa ttaatggcat cattatattt
     2581 tctttattcg tcattgttgg taaaagatta aaatcttgaa aaacaaaacc taccatttgt
     2641 tgacgataca atgctttact tttatttttc atattattta aatgtgcgcc atccacaata
     2701 atgtcacctt cagttagtcc atcaaaagaa gcaattaaat ttagtagtgt agacttccct
     2761 gacccagact cacccataat tgcaacaaat tcaccagctc caactgataa attcatttga
     2821 ttaagtgctg tcgtagcatt caaaccttta ccataaacct tttttacatg tttcacttct
     2881 agcaacatgg catgcacctc atttctttgt tagttctata ttaaaacaaa aaaatactaa
     2941 taacatttaa attagtaaaa aaatgttact agtatactta caatattgaa agtttttatt
     3001 catctggaaa ttgaatcgta aatgttgtac cctcattttg tttagatacg actgaaacag
     3061 gatggtttgt gtgtgttgaa atttgtttta cgataaataa accaatccca cttgagttac
     3121 tttggcgctg gccattataa cctgaatagc ccttatcaaa tattttaggc aagtccgctt
     3181 cactaatacc gataccatta tcttttacgt gtaattgatt ggattgctca tcaaattcaa
     3241 tccatatatc tttacctctc gcatacttaa gtgcattatt tattaattgt tctatcatta
     3301 aagaggtcca tctaacgtca gttaatactt cgtgatgaca aggttcataa tggatttttg
     3361 tttttt
//
LOCUS       DAKTLH010000193         3355 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.197, whole genome shotgun sequence.
ACCESSION   DAKTLH010000193 DAKTLH010000000
VERSION     DAKTLH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.197"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..466
                     /gene="sdpA"
                     /locus_tag="O0394_002554"
     CDS             <1..466
                     /gene="sdpA"
                     /locus_tag="O0394_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpA"
                     /protein_id="HCY0500601.1"
                     /translation="LRLFKINLFSFKKLTLSQVIYIIGGALLIYGLDNLYLYFHDVPA
                     NEQQLEQAIRNTPFYISIFTVTIIPAIVEEIVFRGMIIRVIFRKHLFLGLIVSSLVFA
                     SLHESDTWIGYLPYLYSGLIFGLIYIKTKRLEVVIFMHFLNNLLALLFIIWR"
     gene            complement(906..1619)
                     /locus_tag="O0394_002555"
     CDS             complement(906..1619)
                     /locus_tag="O0394_002555"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCY0500602.1"
                     /translation="MAKLNIEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
     gene            1879..2181
                     /locus_tag="O0394_002556"
     CDS             1879..2181
                     /locus_tag="O0394_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500603.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGLFNSFIKLILFCSV
                     INSIVLILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVRTFGFI"
     gene            2437..2802
                     /gene="crcB"
                     /locus_tag="O0394_002557"
     CDS             2437..2802
                     /gene="crcB"
                     /locus_tag="O0394_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCY0500604.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGDLS"
     gene            2799..3152
                     /locus_tag="O0394_002558"
     CDS             2799..3152
                     /locus_tag="O0394_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HCY0500605.1"
                     /translation="MISIILVMIGGGFGAIARSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
BASE COUNT         1054 a          548 c          449 g         1304 t
ORIGIN      
        1 attgaggcta ttcaaaatca atttattctc ttttaaaaaa ttaacattgt ctcaagttat
       61 ttatattata ggcggtgcac tattaattta tgggttagat aatctttatt tatattttca
      121 tgacgtaccg gcaaatgaac aacaattaga gcaagcaata cgaaatacac cattctatat
      181 ttctattttc actgttacca tcatccccgc tattgtggaa gaaattgttt ttcgcggtat
      241 gataataagg gttatcttca gaaaacactt gtttttaggg ttaattgtgt ctagtttagt
      301 ttttgcatca ttacacgaat ctgacacttg gattggttat ttaccttact tatattctgg
      361 tttgattttt ggcctaattt atataaaaac aaaacgatta gaagttgtaa tattcatgca
      421 cttcttaaat aacttgttag ctctgctctt tataatatgg agataatttt gagataacct
      481 ttcatattat tttcaaatta tctatctgat atttatttta ttgaacttga gtcaccactt
      541 aaactcaaaa atacgtgtca tcaagtgcaa aataatttag aactaataca aacataaaaa
      601 cttttacttt acgcttaaaa tcaaaagtca catacctaga tataagtaca acttgtatat
      661 tttgtactct aataggtatg tggcttaaat aatatttaat tcttgagttg catatagtaa
      721 ctgtttttat tcattttcta aaatatgcaa gactaatttc cttttctact cctgttgaat
      781 caatttagac ttttttataa ctcgagcttt tacttagctg aattttgact tcttcttcat
      841 taataactat gatggaaaaa cccccataag cacaatgcct atgagggttt aaatatattt
      901 atatattata gaatagaaag acctgaagat tgaatatctt tcgcaaagcc tttaactgta
      961 tctactgata attcgttaat atcgcgacct aagtttgtat tcactttttt cacaacatct
     1021 gctgggcatg taataatatc tgcaccaatt tcatcagctt gaatcacatt gaataattcg
     1081 cggcaacttg cccataataa tttaacgccg tctttactat gcgtaacttt tacagcctct
     1141 ttcattaatg gtaatggatc tacgcctgta tctgcaatac gtcctgcaaa tactgaaaca
     1201 tatgttggca caccttcagt tactgcttca gttatttctt taacttgttc aattgtgtaa
     1261 acagccgtaa cgtttaatct cacattgtca gctgaaagtt ttttaattaa aggaatcgtt
     1321 gattcacctt ttgtatttac aataggaatt ttaacaaata cattttcacc atattgtttt
     1381 aaaattgctg cttctttttc catagtttct aaatcgtctg caaatacttc aaatgaaatt
     1441 gaagcatctg gaatttcttt cacagcttct tcagcaaaag ctttgtaatc tgttacgccc
     1501 gctttagcca ttaaactagg atttgttgta aaaccatcca cttgtttgtt tttataagct
     1561 gctttcattt cttcaatatc tgcaccgtcc gcaaatactt ctatatttag tttagccata
     1621 taatatagcc tccttgattc ttattaaaat tttaacaaca tctgcatgtc tttttcttac
     1681 aaccatttgt aaaaaatgat ttttatttct ttgttatata aaacatttat atcacgcttt
     1741 tataatatat agctaatttt ttgcctatat tttttaaaaa tgactttagc aaacattgtg
     1801 tctacaagtt aataaaacta ttgttttatg attgtttatg atacgatatg taaatgataa
     1861 tcatccagga ggtctagcat gtctcgttca aaaaaatact tttacttatc tagcttaatg
     1921 attattttaa gctttttctt taatacaaat aacgttttcc taagtggact ttttaattct
     1981 tttattaaat taatactttt ctgcagtgtt attaactcaa ttgtactaat tttgtctata
     2041 atttttgcag atcgttcaat taaatcacta aagcctgatg cagattggat tagaattgcg
     2101 agtaaaagtt tgccttggat tattctaatt gttattttag tacatatctt ttcaattgtt
     2161 cgtacattcg gttttattta aaaaagttaa tatgtcattg tagccttaat acaaaacaat
     2221 acaatgtatc atgctataat gagtaaaaca atttgataac gttgttgcgt ataaaaaata
     2281 ttagattttc gaaatcataa ctatgcatct aatcgctata gttatacaac aagatataac
     2341 atataatgag gtttgataat gcatcgacaa tttttgtcgt cgcgttgcca aaacctcttt
     2401 tttaaattca aactacttct tttcgaggtg aaccaaatgc aatatgtata tatttttatc
     2461 ggtggtgctt taggcgcttt attacgttac ctcatttctt ttctgaatac tgacggaggt
     2521 tttccaatcg gaacactgat agccaatttg actggtgcct ttgtaatggg attgctaaca
     2581 gccttaacaa ttgcattttt ttcaaaccat ccgaccctaa aaaaagctat tacgactggt
     2641 tttcttggtg ctttaacgac tttttcaaca tttcaattag aattaataca tatgtttgat
     2701 catcaacaat ttataacttt actactatat gctgtaacaa gttatgtctt tggtattttg
     2761 ttatgttacg tcggtataaa actaggtggt gatttatcat gatatcaatc attttagtca
     2821 tgattggcgg cggttttggc gcaattgcta gaagtgccat tactgattat tttaatcata
     2881 aatttacttc aaagttacct atcgcaacat tgatagtaaa tctagttggt agttttttaa
     2941 ttggattaac tataggctta tcaatttcaa tctcatggtt ccctgcgttc tttgttaccg
     3001 gttttttagg tggcttaaca actttctcaa cgttagccaa agaacttaca ctaatgatga
     3061 cgccaaaatt taatattaac ctttttctca attattcact tttacaattc atcattggat
     3121 ttatagcttg ttatattggc tatcatattt aaaaataaaa tgcttcattc agcaaatagg
     3181 taaattacga caccttcctg aacgaagcat tttttaattt tcatgcaaat ttttaagcac
     3241 catataatgc ctaccaaatt tcaataatct ttgttgccgt ttaaataatg tgaatgtcaa
     3301 taaattctcc aaactagtcg aaaataaagg gagtgggaca taaatcccta aaaaa
//
LOCUS       DAKTLH010000194         3262 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.199, whole genome shotgun sequence.
ACCESSION   DAKTLH010000194 DAKTLH010000000
VERSION     DAKTLH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.199"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(302..949)
                     /locus_tag="O0394_002559"
     CDS             complement(302..949)
                     /locus_tag="O0394_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0500606.1"
                     /translation="MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITH
                     YMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQRAGMQSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            complement(1062..1610)
                     /gene="hxlB"
                     /locus_tag="O0394_002560"
     CDS             complement(1062..1610)
                     /gene="hxlB"
                     /locus_tag="O0394_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HCY0500607.1"
                     /translation="MAKFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQLLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            complement(1612..2244)
                     /gene="hxlA"
                     /locus_tag="O0394_002561"
     CDS             complement(1612..2244)
                     /gene="hxlA"
                     /locus_tag="O0394_002561"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HCY0500608.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIIDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKDIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            complement(2321..3079)
                     /gene="nagB"
                     /locus_tag="O0394_002562"
     CDS             complement(2321..3079)
                     /gene="nagB"
                     /locus_tag="O0394_002562"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCY0500609.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
BASE COUNT         1003 a          618 c          442 g         1199 t
ORIGIN      
        1 actttttgga attataacta aaaatagcga tagcacataa cttcatgtaa gtgatatgct
       61 atcgccattt tcgacatcgt atttaaatgt ttatactcaa tttacttcaa taactttgtc
      121 attactttaa tttattttta caaaaataaa ttatcaacga gtcagacatt actctttaaa
      181 aattcataat gtttaataaa tatttatttt aaattcctaa tctaaagcga aatatttcta
      241 agtataatct aatatttatg aatatgcatt ttaaccatgg tattctctgt tttaacaagt
      301 tttataatac ggtattaatt tctgatggat cattaataat aaaatccgga tttgaatgaa
      361 gtaatgacct tgcatcatgt gcaccccaag tgacagctgc agattgcata ccagcacgtt
      421 gtgccatctc aacatcaaac gttgaatcac caatatacac cgtttgttgg ctatttaaat
      481 tgtagcgttg cacaattgtg tgtatgcctt caggatttgg tttatatgca cttacttgat
      541 cggatccaac agcttcggta atcaagtgat ttaatccaat agccgataaa tttctttcta
      601 atacatcact cttcttacta gacaccacga aaagtttttt cccttggtta tacaaacttg
      661 taatggcttc agttataccc gcaaattcat aaatatagtc cttttcaata gattgatatg
      721 tatgtctaaa tgtatcgatt aactttgcta atgctgcttc atctaatggt cggtctgcta
      781 attttaaaaa tgattcttca ataggtattc ccatataatg cgttatttct ttagatgatg
      841 gttccgttaa gccacatgct ttaaatgcac tttgtgttgc tacttcacca cattttttcg
      901 tgtctgccaa cgtaccatca aaatcaaaaa tataattgtc aaacttcaat attaacaccc
      961 ctattcgttt attattgata ttatactata atttattatt taaataaaaa aataatcgat
     1021 aatatttctg cctaggcatc atattatcga ctatctttat tttattctaa attagcatga
     1081 ttttgttgca tcgtttgttc cgtaacattc atttcagtca tcaatcccat tacaacacta
     1141 tctaaaaata attgagatgc ttgttcaaac aaacttccta atagttgtgc cgagccttgt
     1201 tcatcatatt ttgtacctgc aggcaaaacg atgttcgtcc cagctagatt gcctattgca
     1261 gaatctttat ttgtagtaat taatacgatg tcagcaccta ctgattttgc tttgtctgct
     1321 aataatctta aatgttccgt ggaacctgaa ccagagataa ttacaaatac atcattcgac
     1381 ttaatcgcag gtgtcgttga ttctccaaca acatgtgcct gtttgccgag ctgatttaag
     1441 cgcattgcaa aactattcgc cacgaatcct gaacgtcctt tgccagctac aaatatatgt
     1501 tcagcatgta gtattttgga tgcaaaagtt gaaaactcat ccgcttcaac atgtgacaaa
     1561 gtcatcttta attcatctag aattaattga tagtcactaa atttagccat attacttacc
     1621 ttcgattgca gcgcgacatt gttttgcagc ttctactgga tcatctgcat ttgcgattcc
     1681 gccaccaaca ataacaagat caggactttc agcgacaata tctttaattg tatctggttt
     1741 aattccacct gctactgcaa ctttagaatt tttaataaca gatttaacgg ttcttaaact
     1801 ttctaatggt gattgccctt ctgcttgtaa atcataacca gtgtgtactg caatataatc
     1861 agcacccatt tcatctagtt cttttgcacg tttttctaaa tcttgaacag caatcatatc
     1921 aactagtaat tgtttattat ttttatgagc ttcttcaata gctgctttaa ttgatgcatc
     1981 ttctgcaaca cctagtattg taattacatc cgcgccaaat ttaattgctt ggctaacttc
     2041 ataatcagct gcatctataa ttttcatgtc tgctaatact tttacattac taatgttgtc
     2101 tgccatatgt ttaactgctg gtaaaccttc gttgtaaatg attggcgtac cgatttctac
     2161 gatatctaca taatctttta ctttatttgc taactcagcc gcgtcttctt tgtttaataa
     2221 atcaattgct aattgtaatt ccactaaaaa tcatccttcc aatgcttttt atctttctat
     2281 tggaaccttt cccgaggcat ttcttaatta aacattgcaa tcaatccatt tcatcgacat
     2341 gaattttcgc aacatttttc gggcaagctt cgtcgtctaa atatatctca acattcgggt
     2401 gtttgtgaag taatgtggct ggaacatcaa cagaaatttc ctgatttaat aaatgtgtaa
     2461 tagcagcacg tttcttttca ccaaatgcga gtaaaatgat acgtttggct tgaagaatat
     2521 tagcaagtcc catcgaaatg gcttgctttg gaacatcatc ttcgttttta aaatatcgac
     2581 tattagcctt aatagtactt tcagtcaaat caacgatatg agtaacgcta tcaaacggcg
     2641 taccaggttc attaaatcca atatgaccat tttcaccaat acctaaaatt tgaatatcac
     2701 gttgaccttg ttgttctaaa acgtcattat attttgacgc ttccgcattc atatcatcgg
     2761 catctccatt tggaatatga atgttctttc tattaaaata aggatattgt ttgaaaagca
     2821 tgtcatccat ataatagtga taactttgcg gatgtgatgc ggttaaacct acatattcgt
     2881 ctaaattaaa cgtggataca ttgtctacgt ttaactgatt tttatttaat aacttaacaa
     2941 gttgttcata caaatctgtc attgtaccac cagttgctaa acctagttta cagtgctgat
     3001 taaatgccat ctctttatat aactcacatg caacatagaa tgatgcttgt tttttcgatc
     3061 ctaagtttaa tactttcatt gtttacatgc ctccacaaat ttaatacatt ttaaaaactt
     3121 atgaattcat tcatacacca ttaatatagc atcattttta atgtttaaca cttttcaaat
     3181 tccaacaaat ttttgatttt taaaagtaaa aaactaatgc tgtatgatta cgtaatcctg
     3241 agtaattacc cttcatgaaa at
//
LOCUS       DAKTLH010000195         3209 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.201, whole genome shotgun sequence.
ACCESSION   DAKTLH010000195 DAKTLH010000000
VERSION     DAKTLH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.201"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..281
                     /locus_tag="O0394_002563"
     CDS             <1..281
                     /locus_tag="O0394_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HCY0500610.1"
                     /translation="SSQILMLTIMNLNFELNEVSDYITVLINKLISHYKEDYLVNFID
                     INENKQDVFKSRKVGLLEGELGVYLTLMTLKNTKILNEKNWTNAFLIS"
     gene            297..815
                     /locus_tag="O0394_002564"
     CDS             297..815
                     /locus_tag="O0394_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HCY0500611.1"
                     /translation="MGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFI
                     NGEILKQFCDNYYDEFEDPFLNHVDIANKHDKIIILPATSNTINKIANGICDNLLLTI
                     CHTAFEKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKTFKKNV
                     VAPEPYKVLEFI"
     gene            825..2198
                     /locus_tag="O0394_002565"
     CDS             825..2198
                     /locus_tag="O0394_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HCY0500612.1"
                     /translation="MKIIKRAIISLIILSLLISITMSNASASEELYYSVEYKNTATFN
                     KLVKKKSLNVVYNIPELHVAQIKMTKMHANALANYKNDIKYINATCSTCITSEKTIDR
                     TSNESLFSRQWDMNKITNNGASYDDLPKHANTKIAIIDTGVMKNHDDLKNNFSTDSKN
                     LVPLNGFRGTEPEETGDVHDVNDRKGHGTMVSGQTSANGKLIGVAPNNKFTMYRVFGS
                     KKTELLWVSKAIVQAANDGNQVINISVGSYIILDKNDHQTFRKDEKVEYDALQKAINY
                     AKKKKSIVVAAAGNDGIDVNDKQKLKLQREYQGNGEVKDVPASMDNVVTVGSTDQKSN
                     LSEFSNFGMNYTDIAAPGGSFAYLNQFGVDKWMNEGYMHKENILTTANNGRYIYQAGT
                     SLATPKVSGALALIIDKYHLEKHPDKAIELLYQHGTSKNNKPFSRYGHGELDVYKALN
                     VANQKAS"
     gene            2221..2913
                     /locus_tag="O0394_002566"
     CDS             2221..2913
                     /locus_tag="O0394_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding subunit"
                     /protein_id="HCY0500613.1"
                     /translation="MAKLVTENISKRFKNQDVLKHINITLENNEVYGLLGINGAGKTT
                     LMKIICGILQQDSGEIKLDNRPMTRNDLHKVGSLIETPATYNHLSAQDNLKIVCLNES
                     VDFSEINSVLSLVNLNVDKKKKVKDFSLGMKQRLGIAMALIKKPEILVLDEPSNGLDP
                     YGIQELRELLKLLTEQGTSIIISSHILSEIQVLADHIGIIHEGELKYQQRNNKDENLE
                     EIFFKITKGDYK"
     gene            2910..>3209
                     /locus_tag="O0394_002567"
     CDS             2910..>3209
                     /locus_tag="O0394_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HCY0500614.1"
                     /translation="MIHLKIEGIKFKNSFSMYVLLISPLVFLCFAIFTVLFAKSNTGT
                     ANSVSPYITLLFNIWPIAFIPIVLCMACNSLFKIEMRNKSFNYYLSNNWSITKEIR"
BASE COUNT         1237 a          404 c          565 g         1003 t
ORIGIN      
        1 tgtcatcaca aatattaatg ttaacaatta tgaatttgaa ttttgaatta aatgaagtgt
       61 ctgattatat cactgtatta ataaataaac tgatttctca ttataaggaa gattatctgg
      121 tgaattttat agacattaat gaaaataagc aagatgtatt taaaagtagg aaagttggcc
      181 ttttagaagg tgaattaggg gtctatctaa cattgatgac actgaaaaat acaaaaattc
      241 tcaatgaaaa aaattggacc aatgcgtttt taattagctg aatggaggcg tatatcatgg
      301 gagaaaatgt attgatttgt ttgtgtggct cagttaatag tatcaatatt agccactata
      361 ttattgaatt aaaatcaaaa tttgatgaag taaatgtaat cgcgtcaaca aacggtagaa
      421 agtttattaa tggtgaaata ttaaagcaat tttgcgataa ctattatgac gaatttgaag
      481 accctttttt aaatcatgta gatatagcaa ataaacatga caagattatt attttacctg
      541 cgacttctaa tacgattaat aaaattgcaa atggtatatg tgacaattta ttattaacta
      601 tttgtcatac agcttttgaa aaactttcta tatttccaaa tatgaattta cgaatgtggg
      661 aaaatccagt tactcaaaat aatattcgat tattaaaaga ttatggtgta tcaatatatc
      721 cagcaaatat ttcagaaagt tatgaattag cgtcaaaaac atttaaaaag aatgttgtcg
      781 cacctgaacc atataaagtt ctggaattca tttgagatta gaatatgaaa atcataaagc
      841 gcgctattat tagtttaata atattgagtc ttttaatatc tattacaatg agtaatgcat
      901 cagcgtcaga agaactatat tacagtgttg aatacaaaaa tacagcaaca tttaacaagt
      961 tagttaaaaa gaagtcctta aacgttgtct ataatatacc ggaattacat gtggcacaga
     1021 ttaaaatgac gaaaatgcat gctaatgctt tagcaaacta taaaaatgat attaaatata
     1081 tcaatgccac atgttcaact tgtattacta gcgagaaaac aatagacaga acatcaaatg
     1141 agtcattatt ttcaagacaa tgggatatga ataaaataac caataatggt gcatcgtatg
     1201 atgatttgcc aaaacatgct aacaccaaaa tagcaatcat agatacaggt gtgatgaaaa
     1261 accatgacga tttgaaaaat aatttctcga ctgattctaa aaatttagta cctttaaacg
     1321 gttttagagg tactgaaccg gaggaaacag gtgatgttca cgatgtcaat gataggaaag
     1381 gacatggcac gatggtgtcg ggtcaaacga gtgctaatgg taagttaata ggtgttgcac
     1441 cgaataacaa atttacaatg tatcgcgtgt ttggtagtaa aaaaacagaa ctgctttggg
     1501 tatcaaaagc gattgttcaa gctgcaaatg atggaaatca agtcattaat attagtgttg
     1561 gtagttatat tattttggac aaaaatgacc atcaaacatt tagaaaagat gaaaaagtag
     1621 aatacgatgc gttacagaaa gcaatcaatt acgccaagaa gaaaaaatct atcgttgttg
     1681 ctgcagctgg taatgatggt attgatgtca atgacaaaca gaaactaaaa ttacagcgtg
     1741 aatatcaagg taatggcgaa gtgaaagatg ttcctgcatc tatggacaat gtcgttacag
     1801 taggatctac agatcaaaag agtaatctat ctgagttttc caattttggt atgaattata
     1861 cagatattgc tgcgcccgga ggatcatttg cttatttaaa tcaattcggt gtggataaat
     1921 ggatgaatga agggtatatg cataaggaga acattttaac tactgccaat aacggaagat
     1981 atatttatca agctggaact tcattagcca cacctaaagt ttcgggagca ctagctttaa
     2041 tcattgataa atatcatctt gaaaaacatc cagataaagc gattgaattg ttatatcagc
     2101 atgggacatc taagaataat aaaccattta gtagatatgg gcatggtgag cttgatgtgt
     2161 ataaagcatt aaatgtagca aatcaaaaag caagttaata aatcaaagga gtttttgatt
     2221 atggcaaaat tagttactga aaacatttcg aagcggttta aaaatcaaga tgtattaaag
     2281 catattaata tcactttaga aaataacgaa gtttatggat tacttggtat taatggagcc
     2341 ggtaaaacga cacttatgaa aattatatgt ggcatacttc aacaagattc aggggaaatt
     2401 aaattagata atagaccaat gacacgaaat gatttgcaca aagttggttc gcttattgaa
     2461 acacctgcga catataatca tttaagtgca caagataatt tgaaaattgt gtgtttaaat
     2521 gaaagcgttg atttcagcga aattaatagt gttttaagct tagtcaattt aaatgtcgat
     2581 aaaaagaaaa aggttaagga cttttcttta ggtatgaaac aaagacttgg aattgcaatg
     2641 gcattaatta aaaagccaga aattttagta ttagacgaac catctaatgg tttagaccca
     2701 tatggaatcc aagaacttag agaacttcta aaattattaa cagaacaagg tactagtatt
     2761 attatttcaa gtcacatttt atctgaaatc caagttttag cagatcatat cggtattatt
     2821 catgagggtg agctaaaata tcagcaaaga aataacaaag atgaaaactt agaagagata
     2881 ttcttcaaaa taacgaaagg tgattacaaa tgatacattt aaagatagaa ggtatcaaat
     2941 ttaaaaattc tttcagtatg tatgttttat taataagtcc gctggtattt ctttgttttg
     3001 ctattttcac agtcttattc gccaaaagta atacgggaac agcgaatagt gtgtcaccat
     3061 atataacttt actatttaat atttggccaa ttgctttcat cccgattgta ttatgtatgg
     3121 cttgtaattc gttatttaaa attgaaatga gaaataaatc atttaattat tacttaagta
     3181 ataattggtc gattacaaaa gaaataaga
//
LOCUS       DAKTLH010000196         3106 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.202, whole genome shotgun sequence.
ACCESSION   DAKTLH010000196 DAKTLH010000000
VERSION     DAKTLH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.202"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            207..857
                     /locus_tag="O0394_002568"
     CDS             207..857
                     /locus_tag="O0394_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HCY0500615.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNQSVKNSERRGMLKGCGGCLISF
                     ILLIILLSACSMMFSNNDNSTNNQSSKTQLTQKDENKNEDKPEEKSETATDEDLQSTE
                     EVPANENTENNQHEIDEITTKDQSDDDINTPNVAEDKSQDDLKDDLKEKQQSSNHHQS
                     TQPKTSPSTETNTQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
     gene            938..1933
                     /locus_tag="O0394_002569"
     CDS             938..1933
                     /locus_tag="O0394_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCY0500616.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVSYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDKSSVEKSESNEETAPVEPMPHSKPTTSEAPPSQNIHNEDSMYDASTE"
     gene            2009..2635
                     /locus_tag="O0394_002570"
                     /pseudo
     CDS             2009..2635
                     /locus_tag="O0394_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2676..3020
                     /locus_tag="O0394_002571"
     CDS             2676..3020
                     /locus_tag="O0394_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HCY0500617.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIENAEHTLFTPYM
                     LETLSSLGVKDSIVDLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLKQYKNVEFND
                     KILINWRIIQEK"
BASE COUNT         1271 a          497 c          513 g          825 t
ORIGIN      
        1 tgtcatgttc gtgtcatttt gagtcgtaat acaactttac aattattaaa ttaacacttc
       61 tacaatccaa aaatattttt aaagttttga aaaaaattaa agtatttcca ctactaattt
      121 ggtaaaatat gattatgtga tatagtgaaa gcgttttaca cttaataact ccctcttaaa
      181 tgcatccagg ttttatgtag taaatcatga aaataacata taaatataga ggagatttac
      241 ctttgaatac agagaacaac aagaatcaaa accaatctgt taaaaattct gaaagacgcg
      301 gcatgttaaa aggatgcggc ggttgcctta tttcttttat tttattaata atcttattat
      361 cagcctgttc aatgatgttt agtaataatg acaattccac taataatcaa tcatcaaaaa
      421 cgcaattaac tcaaaaagat gaaaataaaa atgaagataa gcctgaggaa aaatcagaaa
      481 cagcaacaga tgaggattta caatcaaccg aagaagtacc tgcaaatgaa aatactgaaa
      541 ataatcaaca tgaaattgat gaaataacaa caaaagatca atcagacgat gatattaaca
      601 caccaaacgt tgcagaagat aaatcacaag acgacttgaa agatgattta aaagaaaagc
      661 aacaatcaag taaccatcat caatccacgc aacctaagac ctcaccatca actgaaacaa
      721 acacgcaaca atcatttgct aattgtaagc aacttagaca agtatatccg aatggtgtca
      781 ctgccgatca tccagcatat cgaccacatt tagatagaga taaagataaa cgtgcatgtg
      841 aacctgataa atattaaaca acaagcgaat tgaattcaaa ttgtatttag ctttatgcac
      901 taatcacata gtaaataatg agggagattt tttaggcatg agcaatcaat tcaaaagcga
      961 agaagagcga agacaatggg aacaattcca agctttccaa aatcaacaaa accaacagaa
     1021 ccagcaatac ggacaaaaga aatctaaaaa aggatggttc tggggctgtg gtggttgtct
     1081 agtattattt attttaatta tcatcggtat ttcagcttgt acagctggta ttacaggtaa
     1141 ccttggcgga aatagttcta aagaaacgaa caaaacccat aaaatcggtg aaactgttaa
     1201 aaatggcgac cttgaagtca ctgtaaattc agtggaaact atgaaatctg taggaccatc
     1261 tcttgcacca acaaacgcta aaggtatatt tgtcgttgct gatgtgacga ttaaaaacaa
     1321 aggtaaagaa gcgttaacaa ttgatagttc aatgtttaag ctaaaatccg gtgataaaac
     1381 atttgaagca gataatacag gttcaatgtc tgctaatcaa agtgacaatg gtagtataga
     1441 aaattcattt ttcttacagc gtataaatcc agatagcact gctcaaggta aaattgtttt
     1501 cgatgtgtca gaaaacatag ccaacgcaaa agataaaaaa ttagaagtta tttctagttt
     1561 atttagcgtc aagaagatta catttgattt atccgatgct aaaaaaacat caaaagctaa
     1621 aaaagacaag caagatacag aagtagctgt tgcgagttca aatagcgata atgtaagtta
     1681 tgaagcttcg gctactacac ctgctacaac ttctagtgcg gatactgatt ctgaagatag
     1741 cgaaaagtct agtaaagatg aggataagca gaatgcgtct aaaagtgata aatctagtgt
     1801 agaaaaaagt gaatctaatg aggaaactgc tcctgtagag cccatgcccc atagcaaacc
     1861 taccactagt gaagcaccac ctagccaaaa tattcacaat gaagatagca tgtacgacgc
     1921 ttcaacagaa taaaatttca gggtagctcg gctacccttc ttttacggaa aaattaatta
     1981 tacataatca aatcaaggag ataaaaaaat gaaattcaaa gctatcgttg caatcacatt
     2041 atcattgtca ctattaactg cctgtggtgc taatcaacat aaagaaaata gtagtaaatc
     2101 aaatgacact aataaaaaga cgcaacaaac tgacaacact acacagtcaa atacagaaaa
     2161 gtaaatgaca ccacaagaag ccgaagatat agttcgaaac gattacaaag caagaggcgt
     2221 taatgaatat caaacattaa attataaaac aaatcttgaa cgaagcaatg aacatgaata
     2281 ttatgttgaa catctagtcc gcgatgcagt tggcacacca ttaaaacgtt gtgctattgt
     2341 taatcgacac aatggcacaa ttattaatat ttttgatgat atgtcagaaa aagacaaaga
     2401 agaatttgaa gcatttaaaa agagaagccc taaatacaat ccaggtatga acaatcatga
     2461 tgaaacagat ggtgagtcag aagacattca acatcatgac attgataata acaaagcaat
     2521 tcaaaatgac ataccagatc aaaaagtcga cgataaaaat gataaaaatg ctgttaataa
     2581 agaagaaaaa catgataatg gggcaaataa ttctgaagaa actaaagtta aataatggca
     2641 tactttgatt aatcgtaatt tttaggagaa acgctatgat aacatttgag aatatacaac
     2701 aacttgagaa atacacctta atgactatgc atggtctttt taatcaactt aaactcggca
     2761 ttatttcaat cgaaaacgca gagcatacgc tttttacacc ttatatgttg gaaacgctct
     2821 cttccctagg cgtgaaagac agcattgtcg atttaattca taaagggact gaattagaag
     2881 actttgcggc atttaattta tcaattgaag acacagttac agtctgttta caaagaactg
     2941 aagaactatt aaaacaatac aaaaatgtgg aattcaatga caaaatatta atcaattggc
     3001 gtattataca agagaaatag acatataaaa gtcgaatgta actacgtgag tattgatttt
     3061 attctttgta aattacaagc atttcatatt ataaagtttg aaaaga
//
LOCUS       DAKTLH010000197         3081 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.203, whole genome shotgun sequence.
ACCESSION   DAKTLH010000197 DAKTLH010000000
VERSION     DAKTLH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.203"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            12..182
                     /locus_tag="O0394_002572"
     CDS             12..182
                     /locus_tag="O0394_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500618.1"
                     /translation="MKYKISLAYNLAIIIGSLIILCILISRGYDIYVILIPILTILAS
                     LINLFCDIKKHK"
     gene            complement(290..598)
                     /locus_tag="O0394_002573"
     CDS             complement(290..598)
                     /locus_tag="O0394_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500619.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISFINQTAIADKHK
                     NQIAYIFTLCISLSAVFLMSSILSFEYDFIALAFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(742..1008)
                     /locus_tag="O0394_002574"
     CDS             complement(742..1008)
                     /locus_tag="O0394_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500620.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            complement(1292..2929)
                     /locus_tag="O0394_002575"
     CDS             complement(1292..2929)
                     /locus_tag="O0394_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCY0500621.1"
                     /translation="MDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRG
                     KFGLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANH
                     QITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDAS
                     ELASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVV
                     FTSLHGTSVPIVPELLQSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELA
                     NKSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMI
                     QSIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPF
                     VRDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGFEGKKK
                     INAIMTKFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSPKSNVIRVLFDEG
                     FIALRPSGTEPKIKLYVSLKCPNFDDVAQKINAMIFS"
BASE COUNT         1054 a          477 c          480 g         1070 t
ORIGIN      
        1 tgggaattga aatgaaatat aaaattagtt tggcttataa tttagcaatt ataatagggt
       61 cgctaattat attatgtata ctaatatcta gaggttatga tatttatgta atcttgattc
      121 caattttgac tattttagct agtttaatca atttgttttg tgatattaaa aagcataaat
      181 gattctatat taatgcaact ttgtattcgt acaaggttgt atttttttcg caaacaatta
      241 cttactgaca gcgcatcaat aaatctaacc aacacactat cacaaacatt tatgggtatt
      301 ttgtaatttt ataaaagcaa acgtagaata atgcgataag taataatgct atcgttaaaa
      361 ataagaatgc taatgcaata aaatcatatt caaagcttag aattgaagac attaaaaata
      421 ctgcactcaa acttatacat agcgtaaata tatatgcaat ttggttcttg tgtttatctg
      481 caatggccgt ttgatttata aaagagatta gtgtgaaatc tttagttatt gcgacataaa
      541 tgcctaatat aaccatactt atgcagaata cgatactaaa tacgatgttg acggtcattg
      601 ttgcaccttc ctgatagaat ataatgtact gtcttttagt aatggatgcg tttcttatat
      661 atgtatgttt ctaaaaaaca gtacgttttt atcatttttt aatttattag ttgaatgaat
      721 ggggttatga aaaaggaagt tttaatcttt tgtcatgaaa taaatgggat gaatatcacg
      781 actagaagta atgttacgaa caggagcgta taaatctaga gacgctaaat tcgacatagt
      841 atgttgctca attatggctg atgatgaatt taaagtatgt gcgttggaac tgtcggattt
      901 ttgttcataa tgttttgcat attgcgccat gatgaatagt gtaaaaataa ataaaacaat
      961 aagagatata atgcccataa tcaaaagtat ttgtttagag cctttcatta tttcacatcc
     1021 tttctaaaat atatttgtaa ctaaatttaa aatagttatt tttgtaattc taaacctttt
     1081 tcatcgcgaa aacaattaaa taggtcgcgg tattaattat tatattatta ccgcttaata
     1141 tgaaaaatac atgaaaatta attttctaat atacttttga aaaattatta caaattagcc
     1201 ccttcaaaac gcgaaaacat aaggattcta gtttcaaaag ggctgataag cataaaatga
     1261 aatgtaatat ttcgatgtat aaaattttta attagctaaa aatcatcgca ttaatttttt
     1321 gagctacatc atcaaaattc ggacatttta acgacacata taatttaatt ttaggttcag
     1381 taccagaagg acgtaaagcg ataaatcctt cgtcaaataa gacacgaata acatttgatt
     1441 taggagaatt aatctgcgac gttgtatctt tatccaaatg ataaacctcg ctagttaaat
     1501 aatcttcaat tgctttcact ttgagtcctt gaatctcttg cggtggattt gaacggaatt
     1561 tggtcattat tgcattaatt ttctttttcc cttcaaatcc ttctagcgta tgcgagaata
     1621 atgtatcctc atgtctacca acagtttgat aaatctgttc taattcatct ttcaatgttt
     1681 tgccatataa ttttaactca gaagcgtatt ttataatgag tggcacaatt tgtacggcat
     1741 ctttatcacg tacaaaaggc tctgatagaa aaccgtaact ctcttcaaat gcgaaaatca
     1801 tattttgatg atcatccagt tgtcttattt cctgagcaat aaatttaaag cccgtcagca
     1861 cctctttgta ttcaacatta ttatagcgtg ctaatgactt tgttaactca ctacttacaa
     1921 tagattgaat cattaagcgg tgacgtaatt gagacgtttg ttgaatacga taattaagta
     1981 acagcgcacc aatttggttg ccattaaaat atgtgatatg accgtgtgca tcacgttcag
     2041 caataccaag tcgatctgca tcagggtcag ttgatattaa taagtctgca tgacttttat
     2101 ttgcaagttc gactgcttga tcaaaagcgc gatgatcttc aggattagca ctttgaactg
     2161 agctgaaatt cggatcaggt ttacattgtg cttctacaag attaaattga ttgaaattca
     2221 aagattgtaa taactcaggt acaatcggaa cacttgtacc atgcaaacta gtgaacacaa
     2281 cttgtaaatc agatttagga atatagccaa tcatattttg aatgtgtttc atatagtcat
     2341 cagttacaga tttcggaaaa ggcttgatat aagatgtatt ttgtttagaa ataggtatat
     2401 caatctgtaa tggatcgcca acttcttcga tataacgact tgcaagctca gatgcatcag
     2461 tcgataattg cgcaccatca gaaccatata ctttgatgcc gttatagtct ttcggattat
     2521 gacttgctgt aatcataatg ccagcagtag tattaagatt gcgcaccgcg aacgataatt
     2581 ccggtgtcgt tttatatgta tcaggtaaat aaactgttat ttgatggttt gctaatacat
     2641 tagcaattat ttgggcgaat tcagttgaaa gatgtctaat atcataatga atgactattg
     2701 ttggactgtt tgtttgggca tttaaataac gcgctaaacc taatgccaat ttttcaatag
     2761 taaacttatt aagtcgacct tcaccaagac cgaattttcc gcgtataccc gcagtaccaa
     2821 atgttaattt actttcaaaa ccttctcgct gttcaatgtc agattgctgc tcataaaaat
     2881 gtttaactaa actatcatta gctcgttcta tccataattc tttatccatt gttgctaaac
     2941 atcctttcaa aatctcagtt agacttaata aaacatgaaa actaaagccc ttacatttat
     3001 gtaatgaatt ataaagaaat acgccccaaa agtaaaaaaa cacagcccca agacaatact
     3061 tttcacaagt attatataat a
//
LOCUS       DAKTLH010000198         3074 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.204, whole genome shotgun sequence.
ACCESSION   DAKTLH010000198 DAKTLH010000000
VERSION     DAKTLH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.204"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..867)
                     /locus_tag="O0394_002576"
     CDS             complement(<1..867)
                     /locus_tag="O0394_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCY0500622.1"
                     /translation="MNKLQDTTFLSYLFTIILWGSAFPMIKIALNDFSAESLSAFRLI
                     LATIILLPFVIIKKLPTPELRDIPVIFILGFCGFVIYHTALNFGEALISAGISGILVS
                     TTPIFSSALAYIFLKEHFSKWNWLSSLVAFIGISIISISKDDYTTINVLGVFIILLAS
                     FSESLYFTFQKKYIEKYGFIAFTLYTIMASSPFMLIFIPEIINDIHGATFTSIVSVLY
                     LAIFPTIIPYVLLAYIVKSVGVSDATMSLYLTPIVSLLLSYLLLDELPTTLAIIGGII
                     TLLGVSLSNFFQN"
     gene            980..2377
                     /locus_tag="O0394_002577"
     CDS             980..2377
                     /locus_tag="O0394_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCY0500623.1"
                     /translation="MAKYKDIASDIRDKIITGDWFYGMKIPSHRQLAIQYNVNRVTII
                     KSIELLEAEGFIYTKVGSGTYVNDYLNEAHITNKWSEMMLWSSQQRSQYTVQLINKIE
                     TDDSYIHISKGELGISLMPHIQLKKAMSNTASHIEDLSFGYNNGYGYIKLRDIIVERM
                     SKQGINVGRENVMITSGALHAIQLLSIGFLGQDAIIISNTPSYIHSTNVFEQLNFRHI
                     DVPYNQINEIDTIIDRFINFKNKAIYIEPRFNNPTGRSLTNEQKKNIITYSERHNIPI
                     IEDDIFRDIFFSDPTPSIKTYDKLGKVIHISSFSKTIAPAIRIGWIVASEKIIEQLAD
                     VRMQIDYGSSILSQMVVYEMLKNKSYDKHLVKLRYVLKDKRDFMLNILNNLFKDIAHW
                     EVPSGGYFVWLVFKIDIDIKYLFYELLSKEKILINPGYIYGSKEKSIRLSFAFESNEN
                     IKHALYKIYTYVKKV"
     gene            2567..3040
                     /locus_tag="O0394_002578"
     CDS             2567..3040
                     /locus_tag="O0394_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HCY0500624.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYPKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
BASE COUNT         1162 a          396 c          517 g          999 t
ORIGIN      
        1 attttgaaag aagttactta aactaacgcc tagtagagtg ataattccgc ctataatagc
       61 aagggttgtt ggtagctcgt ctaataacag ataagataat aataaagaaa cgataggtgt
      121 taaataaaga gacattgttg catcagagac accaactgac ttcacaatat aagcaagcaa
      181 aacgtatgga attatagtag ggaatatagc taaataaagt accgatacta ttgatgtaaa
      241 agtggcgccg tgtatatcgt tgatgatttc aggaataaaa ataagcataa atggtgagct
      301 tgccattatt gtatatagtg tgaaagcgat gaagccgtat ttttctatgt attttttctg
      361 gaaagtaaaa tacaaacttt cactaaaaga tgcaagtaaa ataataaaaa cacctaatac
      421 attaatagtt gtgtaatcat ctttacttat tgaaataatg gatattccta taaatgcgac
      481 gagtgaactt aaccaattcc attttgaaaa atgctctttt aaaaatatat aagctaaagc
      541 actagaaaaa ataggcgttg tagagactag aattccagat atacctgcac taatcaaagc
      601 ttcaccaaaa tttaaagctg tgtgatatat cacaaatcca caaaatccta aaataaaaat
      661 aacagggata tctcttagtt caggggtagg caatttcttt ataattacga acggcaagag
      721 aattattgtt gctaaaatta aacgaaatgc cgacaatgat tctgcactaa aatcatttaa
      781 cgcaatcttt atcattggaa atgcagatcc ccacaatatg atagtaaata aatatgatag
      841 aaaagtagtg tcttgaagtt tattcattaa tatcatcact cctttaatta tgtgtttcta
      901 tattaaaaaa tatgatttaa aatgagtaca accaattttg aatggattta cctatccaat
      961 tttaaaaggg agggagaaga tggctaaata taaagatatt gctagtgaca taagagataa
     1021 aataatcaca ggggattggt tttatggaat gaaaatacct tcacataggc agttggcgat
     1081 acagtacaac gtaaatagag taacgattat taaaagtatt gagttattag aagctgaagg
     1141 atttatctat actaaagtag gtagtggaac atatgttaat gactatttga atgaagcaca
     1201 tattacaaat aagtggtctg aaatgatgtt atggtcctct caacaaagaa gtcagtatac
     1261 ggtgcaatta attaataaaa ttgagacaga tgattcgtat atacatataa gtaaaggtga
     1321 attgggtata tcgttaatgc cacatattca attgaaaaaa gccatgtcta atacagccag
     1381 tcatattgaa gacttatctt ttggttataa taatggctat ggttatatca agttaagaga
     1441 tattatcgtt gaacgaatgt caaagcaagg tataaatgta ggtagagaaa atgtaatgat
     1501 cacttcaggc gctttacatg ccattcaact tttatctatt gggtttttag gtcaagatgc
     1561 cataataatt tcgaatacac catcatatat tcactctaca aatgtttttg agcaattgaa
     1621 ttttagacat attgatgttc cttataatca aattaatgaa attgatacca tcattgatag
     1681 atttattaat tttaaaaata aagcgattta tatagaacct aggtttaata acccgacagg
     1741 tcgttcttta acgaatgagc aaaagaaaaa tataattact tatagcgaaa gacataatat
     1801 tcctatcatt gaagatgata tctttagaga tattttcttt agcgatccaa ctccttctat
     1861 caaaacttat gataaattgg gaaaagttat acatataagc agtttttcaa aaacgattgc
     1921 accagcaata agaataggtt ggattgttgc ttctgaaaaa ataatagagc aattggcaga
     1981 tgtaagaatg caaattgact atggatccag tattttgtca caaatggttg tatatgagat
     2041 gttgaaaaat aagtcttatg ataaacactt agtaaagtta aggtatgttt taaaagataa
     2101 acgagacttt atgttaaaca tcctcaataa tttatttaag gatatagcac attgggaggt
     2161 tccaagtgga ggttattttg tatggttagt ctttaaaata gatatagata ttaaatattt
     2221 attttacgaa ttgttaagta aagaaaaaat attaatcaat ccgggttaca tttatggcag
     2281 taaagaaaag agtataaggc tatcttttgc ctttgaatca aatgaaaata ttaagcatgc
     2341 gctctataaa atttatacat atgtgaaaaa ggtttaatta aaacaataat tcgaatcatt
     2401 atgtggcatg ttaaacagct aaatataagc tatgcacatt taacaagcgg atactcctga
     2461 cgcaattata gtatcattac atattgttta atcgtttggg gtaatgtaat taaaaggtaa
     2521 aataggcaga gtgttataca taaaatgtaa agaaagaggc gatagaatgt ctaaaatcag
     2581 gtcttttaca atattaagtc tacttattta cttagctatg atgtgctata cagtagtgac
     2641 ctatccaaaa ttaccaacca aagtgcctat tcattataat ttagcagggg atgctgataa
     2701 ttttgctgat aaatgggtgc tgcttttgat taatagcgca tttatagtga tttggcttat
     2761 atttttcatt gcaggtagat actatgaacg atttgccaaa tggtcacatt ataatcatac
     2821 accacgtgaa attcgagcga ttaaattatt tttaagtacg ttaaatttag agattatgag
     2881 ctatatgtct atcttcacag tattagaaat ttggcaaata caacaccatc atcaattcaa
     2941 tttactatgg tttaatatga tatttatcat tatcattggt ttgacgcttg tcatattttg
     3001 tttacttcct acaattcata aaatgagaga ttctcaataa aaatataaac tctcatgatg
     3061 cagttttgaa gcga
//
LOCUS       DAKTLH010000199         3069 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.205, whole genome shotgun sequence.
ACCESSION   DAKTLH010000199 DAKTLH010000000
VERSION     DAKTLH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.205"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            12..584
                     /locus_tag="O0394_002579"
     CDS             12..584
                     /locus_tag="O0394_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HCY0500625.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDLYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            587..1312
                     /locus_tag="O0394_002580"
     CDS             587..1312
                     /locus_tag="O0394_002580"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HCY0500626.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            1314..2273
                     /locus_tag="O0394_002581"
     CDS             1314..2273
                     /locus_tag="O0394_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCY0500627.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            2365..2814
                     /gene="ndk"
                     /locus_tag="O0394_002582"
     CDS             2365..2814
                     /gene="ndk"
                     /locus_tag="O0394_002582"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCY0500628.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
BASE COUNT         1133 a          406 c          585 g          945 t
ORIGIN      
        1 gaggtgtaga catggaaaca actgttagca aattggaaag acaaatagaa gaaagattaa
       61 agggcgtatc agaatatgaa tccattaata ttaatcatcg gttaggtaag ttacttgatt
      121 tatatgatat tccagatgtt gccaaagtgg catgcctcac aattgacaca tcaatgagac
      181 accttgatga cattacatat aatcatttgt ctaaacattc cattttgatt ggagatttaa
      241 ttagtgcaca tttttatacg ctactagcag aaatcaatga tttatcattt caaaatgaaa
      301 ttagtaaagc aattgttgaa attaatgaat tgaaatcttc attacatcat caagctttaa
      361 atgattatga aatttctcaa gcaatagtaa aaattgaaac acttttccca tatattacgt
      421 tatctcattt cggtatcaat attgatgagt cggaaatata taattattta tttgaagata
      481 tgtctgatta ctatccatca tacttcaaga aatataatca aagtgaagta aagcattact
      541 tacacgatat ccaaaaaagt tatttaaaaa gtagaggtaa ttaagcatgg ctgacaataa
      601 agcaaataaa gagcaagtac atcgcgtttt tcaaaatatt tcaaaaaaat atgatagatt
      661 aaataatatt attagttttg agcagcataa agtatggaga aaacgcgtca tgaaagacat
      721 gggtgttaga aaagggacga aagcattaga tgtttgttgt ggtactggtg attggacaat
      781 cgcattaagc aaagctgtag gaccaacagg tgaagttact ggtattgact ttagtgagaa
      841 tatgttagaa gtaggaaaag aaaaaactgc ttcaatggaa aatgttaaac ttgttcatgg
      901 tgatgcaatg gaattgccgt ttgaagacaa ttcttttgat tatgtaacaa ttgggtttgg
      961 attaagaaat gtgccagact atttagtcgc gttaaaagaa atgaatagag tacttaaacc
     1021 aggtggtatg gtggtatgtc ttgaaacgag ccaaccaact ttgccagtct ttaaacaaat
     1081 gtatgcacta tactttaaat ttgttatgcc tatttttgga aaattatttg caaaatcaaa
     1141 agaagaatat gaatggttac agcaatctac gtttaatttt cctggaaaag aagagttgaa
     1201 gcgcatgttt gaagaagcag gtttcataaa tgtaagagta cgtagtttta cagggggcgt
     1261 tgctgcaatg caccttggct ataaagaaaa agataatacc aaaggtgatt aacgtggcaa
     1321 agttaaacat gaacaatgaa attaagaaag tggaacaacg acttgaaaag gcaataaaaa
     1381 gtaaagattc tgtattagaa caggcatcat tacacttatt gtcttctggt ggtaaaagag
     1441 tacgcccagc atttgttatt ctgagtagcc aatttggcaa agatgaacaa acgtctgaac
     1501 aaacgtatca agttgcagtc gcattagagt taattcatat ggcaacactt gttcatgatg
     1561 acgttattga taaaagcgac aagcgtcgag gcaagttaac catatcaaag aaatgggatc
     1621 agacaactgc tattttaact gggaattttt tattggcatt aggacttgaa cacttaatgg
     1681 ccgttaaaga taatcgtgta catcaattga tatctgaatc tatcgttgat gtttgtagag
     1741 gggaactttt ccaatttcaa gaccaattta acagtcaaca gacaattatt aattatttac
     1801 gacgtatcaa tcgcaaaaca gcactgttaa ttcaaatatc aactgaagtt ggtgcaatta
     1861 cttctcaatc tgataaagag actgtacgaa aattgaaaat gattggtcat tatataggta
     1921 tgagcttcca aatcattgat gatgtattag acttcacaag taccgaaaag aaattaggta
     1981 agccggtcgg aagtgatttg cttaatggtc atattacgtt accgatttta ttagaaatgc
     2041 gtaaaaatcc agacttcaaa ttgaaaatcg aacagttacg tcgtgatagt gaacgcaaag
     2101 aatttgaaga atgtatccaa atcattagaa aatctgacag catcgatgag gctaaggcag
     2161 taagttcgaa gtatttaagt aaagctttga atttgatttc tgagttacca gatggacatc
     2221 cgaaatcact acttttaagt ttgacgaaaa aaatgggttc aagaaacacg tagtatttat
     2281 gaaaagtatt gaaagcgctt taccaacctg ttaatatata atagtaatat acataaacct
     2341 attaaacaca gggggataca taaagtggaa cgtacatttt taatgattaa accagatgca
     2401 gtacaaagaa atctaattgg tgaagtaatt tcaagaattg aaagaaaagg actaaaactt
     2461 gtcggtggta aattaatgca agtaccaatg gaacttgctg aaacacatta tggtgaacac
     2521 caaggtaaac cattttataa tgatttaatt tcatttatta catcagcacc agtgttcgca
     2581 atggtagttg aaggtgaaga tgcagttaat gtatctagac atattattgg cagcaccaat
     2641 ccttcagaag cttcaccagg atcaattaga ggtgatttag gtttaactgt tggtagaaat
     2701 atcattcacg gttcagattc attagagtct gctgaacgtg aaattaatct atggtttaat
     2761 gaaaatgaaa ttactagcta tgcttcacca cgtgatgcat ggttatatga ataaaatata
     2821 aactgtaaac ctttacgatt tatttataaa ggtagaaagg gttttgttat gtggttagtc
     2881 attatgatta tacataacaa ggcccgtttt ttatgttgta gtaaattact tgaaaaattt
     2941 tatagttttt tggtaacacg tattaaaaag agaggaatat tctttatcaa atgaaactaa
     3001 acagagagaa ggggttgtta aaatgaagaa tattatttcg attattttgg ggattttaat
     3061 gttcttaaa
//
LOCUS       DAKTLH010000200         2962 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.206, whole genome shotgun sequence.
ACCESSION   DAKTLH010000200 DAKTLH010000000
VERSION     DAKTLH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.206"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            328..1497
                     /locus_tag="O0394_002583"
     CDS             328..1497
                     /locus_tag="O0394_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HCY0500629.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG"
     gene            complement(1604..2032)
                     /locus_tag="O0394_002584"
     CDS             complement(1604..2032)
                     /locus_tag="O0394_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCY0500630.1"
                     /translation="MILNFNQFENQNFFNANPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS"
     gene            complement(2248..2694)
                     /locus_tag="O0394_002585"
     CDS             complement(2248..2694)
                     /locus_tag="O0394_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0500631.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSKNFDYSDKK"
BASE COUNT          983 a          396 c          491 g         1092 t
ORIGIN      
        1 tgattttcaa gcagtaattg taactaattg aaaaaaatta agaaaagtat gtgagtgttc
       61 ctaaataata tgattaaaat gatggcgaat aagtgtctaa aagcatctta aagggacatt
      121 gtatagggta aatcacttca taaataaggg aaaatcctta tgttcacttt ttcacaatca
      181 taataaaata tatatgtagt caatactttg tctatattga atgttttcat ataaatgaaa
      241 gcatttttaa ataacattga cctctaatat ataggcagag tattgatatc tattaaaaaa
      301 taaatgattt tgatgaaggt gaaacgtatg tacaaaacaa aaggtggctt tcaacttaca
      361 ttacaaacat taagtttagt ggttgggttt atggcttgga gtataattgc gccattaatg
      421 ccctttatta aacaagatgt caatgttact gaaggtcaaa tatcaatcat tttagcgata
      481 ccagttattt tgggatcggt gctccgtgtg ccatttggtt atttaacaaa cattgttggc
      541 gctaaatggg tattctttac tagttttatc gtattgttat tcccgatatt tttcttaagc
      601 caagcacaaa caccgggtat gttaatggct tcaggatttt tccttggtgt aggtggtgca
      661 attttctcag ttggtgttac atcagttcct aaatatttcc ctaaagaaaa agtaggtcta
      721 gcaaatggta tttatggtat gggaaatatc ggtacagcag tttcttcatt tttagcacca
      781 ccgatagcgg gtattattgg ttggcaaaca acagttagaa gttacttaat tattatcgct
      841 ttatttgcat taattatgtt catttttggt gacacacaag aacgtaaaat taaagtacca
      901 ttaatggctc aaatgaaaac attatctaaa aactacaaat tatattactt aagttattgg
      961 tattttatta cttttggtgc ttttgtagca tttggtattt tcttacctaa ctacttagta
     1021 aatcattttg gaattgacaa agtagatgct ggtattcgat caggtgtatt cattgcgctg
     1081 gcaacattct taagaccaat aggtggcatt ttaggtgata aatttaatgc agttaaagta
     1141 ttgatgattg attttgttgt tatgattatc ggtgccatta ttttaggtat ttcagaccat
     1201 atcgcattat tcactgtagg ctgtttaaca ataagtattt gtgcaggtat tggtaacggc
     1261 ttaatcttca aattagtacc atcatacttc ttaaatgaag cgggatccgc aaatggtatc
     1321 gtatcaatga tgggtggttt aggaggattc ttcccaccac tagtaatcac gtacgtagct
     1381 aatttaacag gatcaagtca tttagcattt attttcttag cggtatttgg atgtattgca
     1441 ttatttacca tgcgtcattt ataccaaaaa gaatatggct cattgaaaaa cggttgatat
     1501 gtaatacatg ccattcattt agttaaatac aaagccttaa tatcatgcgc aatattcgta
     1561 gcatgacatt aaggctttag tagtgtattc attttaaatt taactatgaa attggaatag
     1621 atgttgatga agtagtttca ttgcttggtt tgattttagg caaggtaatg gttaataacc
     1681 cattttcaaa actagcagta atatgttgct tatcaacagc ttcaaaatca aattgacgca
     1741 ttaatgattc gaagttacgc tcatctaaaa tgagttgttc agatttgtat tttgcgcttc
     1801 tagtagcttg aatagtgagc gtattattat tgaaatcgat actaatatct tctttattta
     1861 cacctgctag ttcagcttct aagtaatata attcgtctgt ttcatatata ttcgttacaa
     1921 atgaaggtgt tgaaaagtaa ttaaatactt gtttacctaa atctttaaat gtatcacttg
     1981 gattagcgtt aaaaaagttt tgattctcga attgattgaa gttcaatatc atcacttcca
     2041 ttatagaaat ttgtattaca aagccattat atgagtagta ttaaaaaagt taaagtaaaa
     2101 ggggagataa aaaagtaata aatgggtaga tttgtgcctg ataaggaatc agggcaaaag
     2161 aactgagcat cgagatttat acaagcaata aagataatga ataaacatat cgatgcatac
     2221 ttctgcccat caccttatga tagggagtta ttttttgtcg gaatagtcaa aatttttaga
     2281 aacaaaattc cgcaaattat taattatatc tgatgcttct cgttctgaaa tgttgaattc
     2341 attaaagacc ttaacagaaa tttcagcaag agggcttttt attgctttac cttgttctgt
     2401 tagagaaatt tgtaggtttc tttcatcttt ctcttcacgt gttcgaacaa cgtaatcttt
     2461 cttttctaat ttctttagta atggtgtcag tgttccagaa tctaagaaca cacgttcacc
     2521 taattttttg atgttaagtt tttcatcatt ttcaatcgcc attaaaacaa tgtaaccagt
     2581 ataggttaaa tcatattcct taagatagtt tgtgtatttt ttaattattt cttttgaaga
     2641 aacataaaat aaaaaacaca actgtttgct aagatagctg ttttctacat acatccaatc
     2701 actccttgtt aaaataaaca atattatgaa gtgtaatagt tgtcaagtac tgatttcatc
     2761 gcaattattg aattgtctat aattgaattt aaatgttaaa taatgcaaaa tatatcgata
     2821 tcttagtatt ttcatcggta attactgata tgactgattt tttaaattga aaaaaatgta
     2881 attgtatcat ccttaattgt taaaagtagt caattatttt gatagatagt aaaataaaat
     2941 ttgttattaa attttttaaa ag
//
LOCUS       DAKTLH010000201         2957 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.207, whole genome shotgun sequence.
ACCESSION   DAKTLH010000201 DAKTLH010000000
VERSION     DAKTLH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.207"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..1023
                     /locus_tag="O0394_002586"
     CDS             <1..1023
                     /locus_tag="O0394_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCY0500632.1"
                     /translation="KISMKNRFLIMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICL
                     CLSFIPKFGIGKGLFLSLFTSVSAFNNAGFALFKNNLIDYSSDPIVIITISILIIFGG
                     IGHFVVIDFINCKKLSKLSLHSKLVLTTTSILIIIGAITFFLLEQFNTMQHMGLVEKI
                     GNSFFQSVTTRTAGFNSIDIASINKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFI
                     FVLNYIRKENNVSVFNKEISDKHIKLSIVTINISFLFISIITFILSIINPNISLIKLL
                     FEVVSAFGTVGLSMNLTTEYHGITKIIIIFVMLCGKVGLLTLLRTFIPPKSPKNYRYT
                     KGQIYL"
     gene            complement(1876..2319)
                     /locus_tag="O0394_002587"
     CDS             complement(1876..2319)
                     /locus_tag="O0394_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0500633.1"
                     /translation="MSIITRLFNNSDFEKLNQLCKLYDDLGYPTNENDLKKRLKKITN
                     HDDYFLLLLIKENKIIGLSGMCKMMFYEKNAEYMRILAFVIHSEFRKKGYGKRLLADS
                     EEFSKRLNCKAITLNSGNRNERLSAHKLYSDNGYVSNTSGFTKQL"
     gene            complement(2484..2861)
                     /locus_tag="O0394_002588"
     CDS             complement(2484..2861)
                     /locus_tag="O0394_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500634.1"
                     /translation="MFISLAIWSINSMGNKQCISLNLLLSDDTFAVISVMTTLGTAIL
                     GGIAPSIYGIFTSPIDKKSSFRAISDWEQEQRNNVKDLMMLLPFTLLSILIVIFIFQF
                     AYSKLFNTIAIIIFIIILVVMFF"
BASE COUNT         1055 a          483 c          337 g         1082 t
ORIGIN      
        1 aagatatcaa tgaaaaatag attcttgatt atggttacat ggaatattga cgaacctggt
       61 ggtgttatta agctaattaa acacttggct atttatagtt tagtcactga attaattggt
      121 atgatttgtt tgtgtttatc ttttatacca aaatttggta taggcaaagg tttattttta
      181 agcttattca catcagtatc agcttttaat aatgctggat ttgccctttt taagaataac
      241 ttaatagatt attctagtga tccaattgtc attattacaa tctcaatact cataatattt
      301 ggaggtattg gacattttgt cgtgatagac tttattaatt gtaaaaaatt gagtaaatta
      361 tctttacatt ctaaattagt cttaactaca actagtatcc taataattat aggagctatt
      421 acattctttt tattagaaca gtttaatact atgcaacata tgggactagt tgaaaaaatc
      481 ggaaattctt ttttccaatc agtaacaaca cgaacagcgg gttttaacag tatagatata
      541 gcaagcatta acaaatctac cgcattaatg ttaatgctac ttatgtttat tggtggtgcc
      601 cctctcagtg cagctggagg aattaaaata actacttttg cagttgcgtt tatttttgta
      661 ctaaattata tacgtaaaga aaataatgtt tcagtattca ataaagaaat atctgacaaa
      721 catataaaac tatctattgt taccattaat atctcatttc tatttatcag catcattact
      781 tttatattat cgataattaa tccgaacata tcattaatca agttattatt cgaagtggtt
      841 tctgcattcg gaacagtagg gttaagtatg aaccttacca cagaatatca tggtattact
      901 aaaataatta ttatattcgt tatgctttgc ggtaaagtag gactattaac tttattaaga
      961 acatttatac caccaaaaag tcctaaaaat taccgctaca ctaaaggaca aatttatcta
     1021 taaaataact atccatcttt aataagaaat gaactagtgt tttaatctat tacattaaaa
     1081 aaactaccta aacaggtagt tttttattta ttttaggccg attaaatgtc ttatgtcttt
     1141 aattaagtaa aatgcacaag tacaccttta aaaagattta ctcaaaacta aactatacct
     1201 agttaaccgc ttgtgattaa gaaattttaa atgtggtttc ttttaattat gtcaaccacc
     1261 cataaggatt ttaatcaagc actcattcat tcctgcactc gcaatatgca ttacttatag
     1321 aagtggatgc actctcatat tatggcaata ccataactcc cgcaccataa tgatttaaat
     1381 accattatga atctcaatta caaattagca cataaaaaag acgcctccca taagtctagt
     1441 caccaaatgg aaaacgtctc gtaattaaaa tatatttgcg tgcattctaa tttgtactta
     1501 gaatacatca tactcgttca taatgtaatt ttctaattag tcaaaactat atacagtttt
     1561 acatcattcc cggcattatg cactaataaa aacttacttt aatacacgct ttaaagatta
     1621 ttaaatcagc gttttaattt ttagacaacg ctattatttt tagtttttca attctattat
     1681 gtcataatta tgtcactcaa aaactgtttt ccaacattga tttattgttg gaaaatctcg
     1741 aaaatctagt ctatttctcc aacaatcgaa gaattatgcc cttttttctc cctttaaata
     1801 agtcataata cgaggcatac atgcaacatt tacaataaaa tatgttccta catcgtatta
     1861 tacgaatgct cacacttata gttgtttagt aaacccagat gtattgctaa catacccatt
     1921 atcactatat agtttatgtg cagatagtct ttcatttcta ttaccactat ttagtgttat
     1981 tgctttacaa ttcaaccgtt tagaaaattc ttcagaatca gctaataatc tctttccata
     2041 accttttttc ctaaattcag aatgtataac aaacgcaagg attctcatat actctgcatt
     2101 tttttcgtaa aacatcattt tacacatacc acttaaacca attattttat tttcttttat
     2161 caaaagcagt aggaagtaat catcatgatt cgttattttc tttagtctct tttttaaatc
     2221 attctcattt gttggataac ctagatcatc atataattta catagttgat ttaatttttc
     2281 aaaatcactg ttattaaaca atcttgtaat tatgctcata taacccaccc taaaaatatt
     2341 atgtacctat tgttacatac aatagatacg caaaatcatt tctcactgca aatcaggacg
     2401 tttctcagcg taaacaaatc atttctattc ttttgaaaaa tatctgcata tcttcacagt
     2461 aattttataa cagattaata aaattaaaaa aacataacaa caagtattat aataaatatt
     2521 ataatagcta tcgtattaaa tagtttagaa tatgcaaact gaaaaataaa aatcacaatt
     2581 aaaatactaa gtaatgtaaa tggtaatagc atcatcagat ctttaacatt atttctttgt
     2641 tcttgctccc aatcgctaat agccctaaaa ctactttttt tatctatagg cgatgtaaaa
     2701 ataccatata ttgaaggtgc tatacctcct aaaatagcag ttcccaaagt tgtcattact
     2761 gaaattactg cgaaagtatc atccgaaagc aataaattca aactaatgca ttgtttatta
     2821 cccatcgaat ttattgacca aatagctaga gaaataaaca acccaaaatt taaaataaat
     2881 gatatagtaa tagcaattgt ttacaaaaca cgaaattttt catttttatt tatattatcc
     2941 atttttctcc ctttttc
//
LOCUS       DAKTLH010000202         2907 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.208, whole genome shotgun sequence.
ACCESSION   DAKTLH010000202 DAKTLH010000000
VERSION     DAKTLH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.208"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(214..359)
                     /locus_tag="O0394_002589"
                     /pseudo
     CDS             complement(214..359)
                     /locus_tag="O0394_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
     gene            complement(592..1194)
                     /locus_tag="O0394_002590"
     CDS             complement(592..1194)
                     /locus_tag="O0394_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCY0500635.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSSLFIYLDNWWI
                     VTFIFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            complement(1209..1385)
                     /locus_tag="O0394_002591"
     CDS             complement(1209..1385)
                     /locus_tag="O0394_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HCY0500636.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            1584..2570
                     /locus_tag="O0394_002592"
     CDS             1584..2570
                     /locus_tag="O0394_002592"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCY0500637.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            complement(2651..2869)
                     /locus_tag="O0394_002593"
     CDS             complement(2651..2869)
                     /locus_tag="O0394_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HCY0500638.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
BASE COUNT         1107 a          430 c          424 g          946 t
ORIGIN      
        1 aatctgacat gtttagcagg caatatacgg aaaatacttc aaaattttgt tacacatcta
       61 ataaaaatag acaattaaat aaaagatatg ttactataaa aatgtaaaaa gacgacatgc
      121 aggaacatgt cgcctaatga gcccgttaaa aagacggtga ctaaatgaga ttttctttaa
      181 ccatcattct atgtcaaagt tttgaaatga tggttatttt ttattgctta aatttattat
      241 tgctactact ataccaatga aagtgacgat aaacataccg aaacttaaca ttaaatgtaa
      301 tgcgtttgca atagatatca caataggctt ttcctttcaa aagtgttaag taatgccata
      361 ggcaccaccc ccctttactc agatagccac catcaattca acttgctcaa aaatattata
      421 ccatttttat aaaatattta gtaataaaaa ctgactaaaa cctaatctca ataataaccg
      481 cttgctgtta aaaaatatta ccactataat gaagcatgcc tcgaatcttc gtattgctat
      541 tcttaagcaa aaaagctttt cttagaacac atacgtctaa gaaaagctta tttaagcatt
      601 aaaaattaat aacaatataa tagctacaat ttgtgcaacg gatgttgttg ctatacgtcg
      661 agtatactca taacttaatg ataatatcac ttgtgcaaca aatataaatg ttactatcca
      721 ccaattatct aaataaataa ataatgaact agaacaaatt gcagttatta atatagctac
      781 ccatttcggt attgaaaaat gtgttaattc tttttgtaaa acagttctca tatataactc
      841 gtgacatgga atgacaaaaa gtaaagttac taacatttgc cacttccaat tgacacctgt
      901 tcttgctaat tctttaatta atgaactata acttaaatta gaagttataa gtgagaataa
      961 aagttgaagc ataattaaaa taattgccgt aataatacca acaccaattg atgtaagcaa
     1021 ccgcttagat tcaatatctc tttgataaaa tacataactg ataccagcaa ttagcattat
     1081 acctgtatat aaataccagt attcttggtg ttcgccccac ataataaata atatgagatg
     1141 aaatataatg aaacttataa taaaccaagt taaagccttc gagattttat tcattataac
     1201 ttaccttctt attcaacaat cttatatcgt ttatgattga taacagtttt ttccggtaaa
     1261 tcaaggtcat taaaattttc cataattgtt aagtcaacaa taacagagtt atcgttgatt
     1321 ttttcaacac gacctcttaa tccatccata aactcaacaa tgttacccac ttctgcaaca
     1381 gtcattttaa gtcctcctta aatcataact aaattatatt gtactaaaat tcacttaaca
     1441 aataaactat taacaatcaa aaataataaa atgtaacttt aagccgatat ttagaatgat
     1501 ttgtgagtat ataattggca ttttgagcaa agttaaggca taaataagta gagaatatat
     1561 atagatataa gggagagatt gtaatgaaat tcattagtaa taataatatt acagatccaa
     1621 ctttaaactt agcaatggaa gaatatgttt taaaaaattt accagcagaa gaaagttact
     1681 ttttatttta cataaataga ccatctatca ttgttggaaa gaatcaaaat acgatagagg
     1741 aagtaaatca gacatatatc gatgctcaca acatcgatgt agtaagaaga atttctggtg
     1801 gtggcgctgt ttatcatgat actggcaatt taaactttag ttttataaca gatgatgatg
     1861 gtaacagttt ccacaatttc caaaaattca ctgaaccaat tgttcaagca ttacaatctt
     1921 taggcgtaaa tgctgaatta actggtcgta acgatattca agttggacaa gcgaaaatct
     1981 cagggaatgc tatggttaaa gttaaaaata gaatgtttag tcatggtacg ttgatgttaa
     2041 atagtgattt agacgaagtt cagaatgcac taaaagttaa tccagctaag attaaatcaa
     2101 aaggtattaa atctgttcgt aaacgggtag caaacattca agaattttta aatgaccctt
     2161 tagaaattga agaatttaaa aaaatcatct tgaaaactat ctttggagaa actgaagtag
     2221 aagaatataa attaacggat gaagattggg aaaatattga aaagttaagt aacgacaaat
     2281 atagaacttg ggaatggaat tatggtagaa accctaaata taactttgaa cgcgaggaaa
     2341 aatttgaaaa aggatttgta caaattaagt ttgatgttaa acgaggtaaa atcgaacatg
     2401 caaaaatatt cggtgatttc tttggtgtcg gagatgtcac tgatcttgaa aatgcattag
     2461 ttggctgtct acacgacttt gagcacatag aagaagcact atcagaatat gatttatatc
     2521 attatttcgg tgacatagat agacatgaat taattagatt aatgtcataa acgattaaag
     2581 gactattctt acatgtatga gatgttaaga atagtccttt tatgattgcg ttatggaatt
     2641 cgaatcaatg ttattcaccg agaaatgcct ccaaattttt gtattctgcc gtatattgat
     2701 taaatgctgc ttcatcttta ttgacgagtg cgtcatctat aagttctatg agctgtcgtt
     2761 ttcttacctc tcgaagtgct tcattgataa ttaattcaat acccaaatca ttgacagttg
     2821 tgacaaacgc ttctaaagtt gtatgtttaa cattagtatt gtatttcata gaaaccacta
     2881 cctttcttta atttataata agtatac
//
LOCUS       DAKTLH010000203         2905 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.209, whole genome shotgun sequence.
ACCESSION   DAKTLH010000203 DAKTLH010000000
VERSION     DAKTLH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.209"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..544
                     /locus_tag="O0394_002594"
     CDS             <1..544
                     /locus_tag="O0394_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0500639.1"
                     /translation="NIANPNLIFIGQKLKVPMTPLVEPKPKTVSSNNKSNSNSSTLNY
                     LKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGAKDIPYANNFNSEAKIYHN
                     TPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKFQSLDQNWNNGGWRKAEVA
                     HKVVHNYENDMIFIRPFKKA"
     gene            572..1816
                     /gene="fmhC"
                     /locus_tag="O0394_002595"
     CDS             572..1816
                     /gene="fmhC"
                     /locus_tag="O0394_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HCY0500640.1"
                     /translation="MKFSTLSEEEFTNYTKKHFKHYTQSIELYNYRNKINHEAHIVGV
                     KNDKNEVIAACLLTEARIFKFYKYFYSHRGPLLDYFDAKLVCYFFKELSKFIYKNRGV
                     FILVDPYLIENLRDANGRIIKNYNNSVIVKMLGKIGYLHQGYTTGYSNKSQIRWISVL
                     DLKDKDENQLLKEMEYQTRRNIKKTIEIGVKVEDLSIEETNRFYKLFQMAEEKHGFHF
                     MNEDYFKRMQEIYKDKAMLKIACINLNEYQDKLKIQLLKIENEMMTVNRALNENPNSK
                     KNKSKLNQLNMQLSSINNRISKTEELIFEDGPVLDLAAALFICTDDEVYYLSSGSNPK
                     YNQYMGAYHLQWHMIKYAKSHNINRYNFYGITGVFSNEADDFGVQQFKKGFNAHVEEL
                     IGDFIKPVRPILYKFAKLIYKV"
     gene            1989..2861
                     /gene="dprA"
                     /locus_tag="O0394_002596"
     CDS             1989..2861
                     /gene="dprA"
                     /locus_tag="O0394_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCY0500641.1"
                     /translation="MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEW
                     VNREENVHLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDSEYPVLLKEIYQFPLLLF
                     YKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
BASE COUNT         1148 a          336 c          470 g          951 t
ORIGIN      
        1 taatatagca aatcctaatt taatatttat tggtcaaaaa ttaaaagtgc caatgacacc
       61 attagtagaa ccaaaaccaa aaacagtgtc ttcaaataat aaaagtaata gtaatagcag
      121 tacattaaat tatttgaaaa cattagagaa tagaggatgg gatttcgacg gtagttatgg
      181 atggcaatgt ttcgatttag ttaatgtata ttggaatcat ctttatggtc atggattaaa
      241 aggatatgga gctaaagata taccatatgc aaataatttt aatagtgaag ctaaaattta
      301 tcacaacaca ccaactttca aagctgaacc tggggactta gtggttttta gtggaagatt
      361 tggtggagga tatggtcata cagctattgt cttaaatggt gattatgatg gaaaattaat
      421 gaagttccaa agtttagatc aaaactggaa taatggtgga tggcgtaaag cagaggttgc
      481 acataaagtt gttcataatt atgaaaatga tatgattttt attagaccat ttaaaaaagc
      541 ataatttaaa tcgaaggcag gacattgaaa tatgaaattt tcaactttaa gtgaagaaga
      601 atttaccaac tacaccaaaa agcacttcaa acattataca cagtctatag aattatataa
      661 ttatagaaat aaaataaatc atgaagcaca tattgtggga gtgaagaatg ataaaaatga
      721 agttatagct gcatgtttat taacagaggc acgaattttt aaattctaca aatatttcta
      781 ctctcataga ggtcctttac ttgattattt cgatgctaaa ttagtttgtt acttttttaa
      841 agaattatct aaattcattt ataaaaatag aggagtattt attcttgttg atccatattt
      901 aatagagaat ttaagagatg caaatggtag gataataaag aattataata attcagtgat
      961 agtaaagatg ctagggaaaa ttgggtatct ccatcaaggt tatacaacag gatattcaaa
     1021 taaaagtcaa attaggtgga tttctgtatt ggatttaaaa gataaagatg agaatcaact
     1081 tttaaaagaa atggaatacc aaactagaag aaatataaaa aagactattg agattggtgt
     1141 taaggttgaa gatttatcta ttgaagaaac aaatcgattt tataaattgt ttcaaatggc
     1201 tgaagaaaaa catggttttc atttcatgaa tgaagattat tttaaacgaa tgcaagaaat
     1261 atataaagat aaggcaatgt taaagatagc ttgtataaat cttaatgaat atcaagataa
     1321 attaaaaata caattattga aaatcgaaaa tgaaatgatg actgtgaaca gagcattaaa
     1381 tgaaaatcca aattctaaaa aaaataaatc aaaattaaat cagttaaata tgcaattatc
     1441 tagtattaat aatagaatta gtaaaaccga agaactaata tttgaagatg gacctgtttt
     1501 ggatttagct gctgctttat ttatatgtac tgatgatgaa gtttattatc tatcaagtgg
     1561 atcaaatccg aaatataatc agtatatggg tgcatatcat ctacaatggc atatgataaa
     1621 atatgcaaaa tcacataata ttaataggta taatttttat ggaataacag gcgtctttag
     1681 taatgaggcg gatgattttg gtgttcaaca atttaaaaag ggttttaatg cacatgttga
     1741 agaattaatt ggtgatttca tcaaaccagt aagaccaatt ctatataaat ttgcaaaact
     1801 tatttataag gtttaattat aaagtatgtt ggaaattgaa attttaaatt ctttccaaca
     1861 tacttttcac tttttaagtc agtattttta ctccttaaaa taacattaca tcgacaatgt
     1921 ttttgtatta gaaatgtaaa acaaaaatag aatatgattt tgtatgatta caaaaaggag
     1981 ttgatttatt gattaaacta tttttgctta agttatactg ggcacacttt tcgactaaac
     2041 aaattcatca atttttaatg gcatatccta atgtaattaa agaggaggga agaaaaaaag
     2101 atagttattt atgtgaatgg gtgaataggg aagaaaatgt tcatttatta cgtaaatact
     2161 atgcttttat aaaacttgat cataacgata ttattaaaga actgcagaaa ttaaaagtaa
     2221 gttacattac atatatggat tctgaatacc cagtgctatt aaaagaaata tatcaatttc
     2281 cattacttct tttctataaa gggaacatca aattaataaa taatatgcat catttggcag
     2341 tagtaggtgc aagagattct acaagttata cccaacagtc tttagaattt ttattatcaa
     2401 atgataaaag caaatattta acaattgttt ccggccttgc tcaaggagct gatgcaatgg
     2461 cacatcaaat agctttaaaa tacaatctcc ctacaattgc agttttagcc tttggccatc
     2521 aaacacatta tcccaaaagt acattagcat taagaaataa aatagaagaa aaaggtttag
     2581 ttatatctga atatccacca catacaccaa tcgctaaata tagatttcct gagcgcaata
     2641 gaattatcag cggtttgtca aaaggggttt taattactga ggctaaggaa caaagtggca
     2701 gtcacatcac gatagatttt gcattagagc aaaatagaaa tgtttatgtt ttacctggat
     2761 ctatgtttaa tcctatgaca aaaggtaatt tattacgtat ccaagaaggt gctaaggtag
     2821 tattaaacgc taatgatata tttgaagact actatattta aaactaaaaa taacgagttg
     2881 cttcatattt cttttgtttc ccttt
//
LOCUS       DAKTLH010000204         2903 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.210, whole genome shotgun sequence.
ACCESSION   DAKTLH010000204 DAKTLH010000000
VERSION     DAKTLH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.210"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            223..483
                     /locus_tag="O0394_002597"
     CDS             223..483
                     /locus_tag="O0394_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500642.1"
                     /translation="MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICG
                     VTLIVSFTIAMITKNWLSEILFFINIVLVLYYIYPIFYSFIG"
     gene            512..700
                     /locus_tag="O0394_002598"
     CDS             512..700
                     /locus_tag="O0394_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500643.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTSKESKKESYLSFTVI
                     LYIFGFAILIYTFIFGVL"
     gene            888..1457
                     /locus_tag="O0394_002599"
     CDS             888..1457
                     /locus_tag="O0394_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500644.1"
                     /translation="MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIVTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKAY"
     gene            1545..1829
                     /locus_tag="O0394_002600"
     CDS             1545..1829
                     /locus_tag="O0394_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500645.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNKGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            1891..2109
                     /locus_tag="O0394_002601"
     CDS             1891..2109
                     /locus_tag="O0394_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500646.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEINLEN
                     GNISNIDHSLLVSVDPKALEISIFDAVN"
     gene            complement(2605..2805)
                     /locus_tag="O0394_002602"
     CDS             complement(2605..2805)
                     /locus_tag="O0394_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCY0500647.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
BASE COUNT         1029 a          431 c          388 g         1055 t
ORIGIN      
        1 taaaaatatt aacgaattat acaactttaa gagtttttag tatgtaaata ttcttttcat
       61 attaatacaa tacaccccta tatatgctca cttgtttgaa ttaataaact ttagcattct
      121 gcaaataata ttttaaaaat aactatactt tttatttacc tattgaaaaa tagtgttatc
      181 atatttatta agaagctatt tttatactag taggtgtcag tcatgaataa cattttgtta
      241 aatgctatca atatagttat tactaccact tttgttatct ttaatatttt aatcacatat
      301 aataaagatt tagatgattt atgttggctc ctgcctggta ttatcatttg tggtgtgaca
      361 ctcatcgtat cctttaccat tgcaatgata actaaaaact ggttaagtga aatattattt
      421 tttataaata tcgtactcgt tctctattac atttatccta ttttttatag ttttataggt
      481 taataattac ggtatataag gagggtttca catgtcttta cattttgcaa ttctgttttg
      541 gctagcatta attttcttag ttgccgctac gtttatactc gtattaatga aaaaaactag
      601 caaagaatct aaaaaagagt cctatttaag tttcactgtc attctctata tttttggatt
      661 cgctatatta atatacacat ttatatttgg tgtgctataa atgaaattca ttttatatcc
      721 agtctgaaac gtctcaatga tgtttcagac tgttttttat tcttaataga catttcacac
      781 tctcatactt taatatttaa gatatgtttt agtcttacaa gcttattaaa gattacaatt
      841 ctaatttaag actatttaca tatatttctt caacaaggag cttttaagtg aactatatta
      901 aacgaacgat aattttactt atattattcg ttgtcgtatc cccaataaac tcaccaaaaa
      961 caattgcaga taataagtat tcagaaattc aagatgacaa attccaatta caacctggtg
     1021 atattattgt tacaaaaggc cctgtcatgt ggggattttt tggtcattgt agtatcgcga
     1081 ttgatgataa aacgatttta caaattgagg ggcctggcga caaaccaact acacaatcct
     1141 tcgaatcttt taaatataat tatgcaagtg gcaaaaatga ttggatgaaa gtttatcgtt
     1201 gcagctatcc tggtgcaggt aaaaaagcgg ctgaatgggt taagaaaaac tatgagaata
     1261 caaatcatcg ttatctagtt acattaaatt tgaacagcaa aaaatttacg tattgtacta
     1321 aaattatcta ccaagcttat aaatttggtg taagtgaaaa atcagttaaa agttatggac
     1381 tacatattat ttcaccctat gcaattaaag ataattttat agatccatac aaactaaggc
     1441 ttgttaaagc ttattaacac agtttcatca aatagttaaa ttagtttgtg ttttgcaata
     1501 aattgggtat agattacaaa tgatatttca ggaggcccaa tattttgaat gaactaacta
     1561 aagaacaaaa gtatacaata gctaaatttt acaaattata tattgaacgt tcgaataaag
     1621 gagaaacaga aacagtcgct aacttttttg gcgatgctaa agatgcacgt gaaaactatt
     1681 tttgcgatcg tgattatcaa gacttcttaa ctaactgcca aattttaatt caaaacaaat
     1741 acttaactgg tgaagttttg gacgataaca tttacaatat ttctatttta aacaagacat
     1801 ttattgaatt cgaacaaaat tttggttgaa catgaatgtc tcaactataa actttctaac
     1861 cctattttaa agcaggagtg aaaaacatac atgagtgaat ggcatattaa taatcaatct
     1921 acaaaacctt ttttaataca acaagcggaa aagcaattgt ctattgttaa accgttgcct
     1981 aatggtagct tccaaatact cacagaaata aatttggaga atggcaatat tagtaacatc
     2041 gatcattctt tacttgtttc agtagacccc aaagcattag aaataagtat atttgacgct
     2101 gtaaattaaa cgcaatattt ttatttaatt attttaatgt ttcgcctttt aaataaaaac
     2161 aagattactg aattattatg agattttaaa aacaataaat attctcaata attaatcagt
     2221 cttaataaaa aattaacaaa aagttgatta gttaaattat taattaagta gtaagatatt
     2281 atgcgatgtg gtatcctatt attgttaaac attttgagtg tttaacatta cttcttgtta
     2341 tcgccatata cagactttaa ttctcgtcat tattaaagtt ctgtattggc gcttttttat
     2401 attcaaaatc aaaagtcgga cagatgaaaa gtaaaaaact tttccatcag ttcgatttat
     2461 tttagaacca ttatatattg ttaaatctcc aactaaattt attggacaaa aaaataacgg
     2521 ctagtgcttt taccacacca actgttatcg atttaaagca taaaaaaaca gccctatagt
     2581 aaggactgtt tgaataagtt aaaattacat tttaactacg tttgcagctt gctcgccacg
     2641 gtcgccttca acgatgtcga attcaacttt ttggccttct tctaatgatt tgtatccatc
     2701 ttcagcgatt gctgagaagt gtacgaatac gtcgctacca tcttctcttt cgatgaaacc
     2761 aaaacctttt tctgcattaa accatttaac tgtaccgtta ttcatattga atacctccgt
     2821 cgtgcttttg cactaaatat tttgtaacaa attcataaat gaaaaggaga atattctata
     2881 taaataactc acatttttct tca
//
LOCUS       DAKTLH010000205         2901 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.211, whole genome shotgun sequence.
ACCESSION   DAKTLH010000205 DAKTLH010000000
VERSION     DAKTLH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.211"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            178..369
                     /locus_tag="O0394_002603"
     CDS             178..369
                     /locus_tag="O0394_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500648.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
     gene            complement(508..693)
                     /locus_tag="O0394_002604"
     CDS             complement(508..693)
                     /locus_tag="O0394_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500649.1"
                     /translation="MLYILITALNVILNKLKMKDSNIISFIYSRHDRILYVNLVGNRF
                     KNNISMTKATSNIGRGF"
     gene            complement(629..910)
                     /locus_tag="O0394_002605"
     CDS             complement(629..910)
                     /locus_tag="O0394_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0500650.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            1199..1894
                     /locus_tag="O0394_002606"
     CDS             1199..1894
                     /locus_tag="O0394_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0500651.1"
                     /translation="MTVLTDKVAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKEKLQ
                     NVANQLSQDSVKVVPTDVTNKEEVDELIKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPTDRKKLEPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
     gene            2145..2567
                     /locus_tag="O0394_002607"
     CDS             2145..2567
                     /locus_tag="O0394_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCY0500652.1"
                     /translation="MNKKHVFIIIGVILCICIVASVIYLKVKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKEPNTIKTVHFTSLKRGPMGDAVIEGYINENKEDDFVAYGSPEHNYQ
                     FGGSLIKSKNLSTLLKPVHQTKSPDEIKKELESKKNDR"
BASE COUNT         1041 a          416 c          495 g          949 t
ORIGIN      
        1 acttttatat aattaaatat ttcttaattt tccaaaatag tgataaattt gtgaaataca
       61 tcacaaatcc ctttatttat ttggaaattc atgtaatatt agacttgtaa gaagttaata
      121 aatagagaga gacgagagag tttatataaa tactatataa acattggagt gatgattatg
      181 agaaaagaga ttgaagcgct tattttctca gacgtaagta gctatgatat ttacgtgaac
      241 actggtgtaa atcaaggatt aattggtgac atcaaagatg gttacctaac tattgattct
      301 atgccttaca ttgatgctga gcgtttgtat cactttgcta tggaacgtaa atcgttagtc
      361 actaactagt tcttattgcc aatgattact acccctagtc ggcggcaatt gaagtgtgat
      421 tgatgtaact tgccctcgtt ggtgagcaat tgagggcaga cccctttaat taagtaaacc
      481 ctaactcccc acaaatctgg aacgatacta aaagccacgt cctatattgg atgtggcttt
      541 agtcatactt atattatttt taaaacgatt acctacaaga tttacatata aaattctatc
      601 atgtctgcta tatataaacg aaatgatatt agaatccttc attttcaact tgtttaaaat
      661 tacgtttaat gccgttataa gaatatacaa cattatataa ttttacttgg gcgtcatgga
      721 cacttgagaa gggcattctt acatcgtcca tatttttaac ttcgtgtcga aatgcttttt
      781 cagctttttg tctttctgca agagacatac ttagccattc ttctttgcta aataaatctt
      841 cgaatcgaaa atcaaatgcg gttcttttac tttctgctct taatgctaaa acacgctcat
      901 gatatgtcac gttttattcc tccctctaat cattcaatcg aatgataaat cacagtatta
      961 actttattac taatataatg tttttacaat aaagaagtga cgatttttca acatttttaa
     1021 aaataaaaaa tataagcaac gattaattgt atgtttcgaa tggcttgtaa tgtaatttat
     1081 ctatgaaaat gaagcgagtt attatgaccg ttatatgaat tgtgatacgc tttttaaaag
     1141 taatatgtat ttatgattta ccctgaaaaa ataaaaagaa tcaaaggagt cgatcattat
     1201 gacagtatta acagataaag tagcagtagt tacaggtgca ggtagtggta ttggagaagc
     1261 aattgcaaca ttactacatg aagaaggggc aaaagttgtc ttagcaggta gaaataaaga
     1321 aaaattacaa aacgtagcga atcaattgtc acaagatagt gtgaaggtag tgccaacaga
     1381 tgtaacgaat aaagaagaag tcgatgaatt gataaaaatt gcacaacaaa cattcggtgg
     1441 tttggatatt gttatcaata gtgcggggca aatgttgtcg tctaagatta ctgattatca
     1501 agtagatgag tgggatagta tgattgatgt gaatatcaaa ggcactttat atacggcaca
     1561 ggctgcatta ccaactatgt tagaacaatc aagtggccat cttattaaca ttgcatctat
     1621 ttctggcttt gaagtaacga aaagtagtac gatttatagt gcgacgaaag cagcagttca
     1681 cactattact caaggattag aaaaagagtt ggcaaagaca ggcgttaaag taacaagcat
     1741 ttctccagga atggtagata cagccataac tgccgcatac aatccaacag atcgtaaaaa
     1801 acttgaacca caagatattg cagaagcagt attatatgca ttaacacaac caaagcacgt
     1861 taacgtgaat gaaattacag tgcgtcctgt ataaaaaggt gtttgaggtc tggggtatag
     1921 gcatttaatg taatagctat cgattttgtt aatgttagta aggcaataat tacatgaggg
     1981 cataataggc aattattcct tgtacaattc tcagtaattg ctgattttaa caatcaagtt
     2041 aatgaaggcg acgatgttag tgttatggtt tataatctgt aatgttcata attatagatt
     2101 ataaaaattt tttcattttc caccacattt taggagttac tttaatgaac aaaaaacatg
     2161 tttttataat tattggtgtc attttatgta tatgtatagt tgcatcagtc atttatttaa
     2221 aagtgaaata tgacgaaaaa gaaaaacaaa aagcaatata ctataaagaa caacaggagc
     2281 gtattacact ctatcttaag cataatacta aagaacccaa taccatcaaa actgtacatt
     2341 tcacaagttt aaaaagagga cctatgggcg atgcagtaat tgaaggttac ataaatgaaa
     2401 ataaagaaga tgattttgtt gcatatgggt ctccagaaca taattatcaa tttggtggaa
     2461 gtttaatcaa aagtaaaaat ttaagcacgt tattaaaacc agtacatcaa accaaatcac
     2521 ctgatgagat aaaaaaggaa ttagagtcta aaaagaacga ccgctaaaac tatgcggccg
     2581 ttttaatttt gttttcaagt taatcatact tacatataaa aatgaaaagt tgaattttta
     2641 gtacaatttc cactgtattt atctgttaac cactttatac ccgcaatatt tcgttgatct
     2701 tatataaaca cttttggctg cttttccttg acctagatta aaattataga ttttctttgt
     2761 aagagtgtgt tttgtagtat cataaatatg taaactgtat aaaattccac cacattttag
     2821 gagctggtga tagatgtcga aaaagaaaat tttaatatta actagtatta tgttaatcat
     2881 attaattagt gttgcaggta t
//
LOCUS       DAKTLH010000206         2894 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.212, whole genome shotgun sequence.
ACCESSION   DAKTLH010000206 DAKTLH010000000
VERSION     DAKTLH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.212"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(37..288)
                     /locus_tag="O0394_002608"
     CDS             complement(37..288)
                     /locus_tag="O0394_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCY0500653.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            634..2457
                     /gene="lepA"
                     /locus_tag="O0394_002609"
     CDS             634..2457
                     /gene="lepA"
                     /locus_tag="O0394_002609"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCY0500654.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCVLRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
BASE COUNT         1020 a          397 c          556 g          921 t
ORIGIN      
        1 agttcaagca tgcgcttgaa cttttattta aaaagattat ttatttgcag tcattaattg
       61 tgatttaata cggtcagctt tgtttgaatg tattaaatta ctttgagcag ctttgtctac
      121 taacttaact gctaagctta ctaattcatt tttattatca gcgttatttg aaacagctgt
      181 tttagcgttt ttaactgctg tacgcattgc actcttttgt gaaatgttgc gtgcttcagc
      241 tttttcagtt gtttttacac gtttaattgc agatttgata tttgccattt ctgtcacctc
      301 ctaaaagtga gttcatctta tcaaaatttt atttgattac aacaagaaat attttaacaa
      361 aagtctaacc gttgtgcaat catttatttg atggggttag cttaaaattt tgcagattta
      421 ataaattgtg ttggaaacac aatcaatacg ttttataatt ataagtacgt aattaacata
      481 ttaacatatc gcaagtatgt atttaaataa agattgttat aatttcaaag ttcatccaag
      541 attatggcgt ttgcatttac ctattaaaaa cgttattata tcaaagatgc gaaagataat
      601 acgggtttat tttatgaaag tgagaaggat aaaatggata atgagcaacg cttaaaaaga
      661 agagagaata taaggaattt ctcgattata gcacatattg accacggaaa atctacattg
      721 gctgatagaa ttttagaaaa taccaaatca gttgaaacaa gagatatgca agatcagtta
      781 ctagattcaa tggatttaga aagagaacgt ggtattacaa tcaaattaaa cgcagtacgt
      841 ttaaagtacg aagctaaaga tggaaatact tatacattcc atttaatcga tacgcctgga
      901 cacgtcgatt ttacatatga agtgtcacgt tctttggcag cttgtgaggg cgcgatttta
      961 gtagtagatg cggctcaagg tatcgaagca caaacattag caaatgttta tttagcatta
     1021 gataatgagt tagagttatt gcctgttatt aacaaaattg atttacctgc tgcagaacct
     1081 gaacgcgtga aacaagaaat tgaagatatg ataggtttag accaagacga tgttgtttta
     1141 gcaagtgcta aatctaacat tggaattgaa gagatactag agaaaatagt tgaagttgtg
     1201 ccagctccag atggtgaccc agaagcacca ctaaaagcgt taatatttga ttctgagtat
     1261 gatccatata gaggggtaat ttcatcgata agaattgtgg acggtgttgt taaagccgga
     1321 gataaaattc gaatgatggc cactggtaaa gagttcgaag taacagaagt tggaattaat
     1381 acacctaagc agcttccagt tgatgaatta acagttggtg atgttggtta tattattgca
     1441 agtattaaaa atgttgatga ttctagggtt ggtgacacca tcacattagc tagtagacct
     1501 gcatcagaac cattgcaagg ttataagaaa atgaatccaa tggtatattg cggactgttc
     1561 ccaatagata acaaaaatta taatgattta agagaagcat tagaaaaatt acaattgaat
     1621 gatgcatcat tagaatttga gcctgaatcg tcacaagcat taggttttgg ttatagaact
     1681 ggtttcttag gtatgttaca catggaaata attcaagaaa gaattgaaag agaatttggt
     1741 attgaattaa ttgcaactgc accatctgta atttatcaat gtgttttaag ggacggttca
     1801 gaagtgacgg ttgataaccc agcacaaatg ccagatcgtg ataaaattga taaaatattt
     1861 gagccatatg ttcgtgcaac tatgatggtt ccaaatgact atgtcggtgc agtaatggaa
     1921 ttatgtcaac gtaaacgtgg acaatttata aatatggact atttagatga tattcgtgta
     1981 aatattgttt atgaattacc tttagctgaa gttgtatttg atttcttcga tcaacttaaa
     2041 tctaatacta aaggatatgc atcatttgat tatgaattca tcgaaaataa agaaagtaat
     2101 ttagtcaaga tggatatttt attaaatggt gataaagtgg atgcgctaag cttcatagtt
     2161 catagagatt ttgcatatga acgtggtaaa gcattagttg aaaaacttaa aacgttaatt
     2221 ccaagacagc aatttgaagt acctgtacag gctgcaatag gacaaaaaat tgtagcgcgt
     2281 acaaatatta aatcaatggg taaaaacgtt ttagctaaat gttatggcgg tgacataagc
     2341 cgtaaacgta aattacttga aaaacaaaaa gcaggtaaag ctaagatgaa agcagttggt
     2401 aatgttgaaa ttccacaaga tgctttcttg gctgtattga aaatggatga tgaataattt
     2461 taaaaaatca attaacaatt tacaatgaat aaagtttaat aactaaaaag agggagccta
     2521 ggataaatta acgtcctggg ctttacaatg ttatattggc agccatcgac agagttaaaa
     2581 tgagcttata acaatggggc cccaacacag aagctgacga aaagtcagct tactataatg
     2641 tgcaagttgg ggtggggccc caacatagag aatttcgaaa agaaattcta caggcaatgc
     2701 aagttggggt gggacgacga aataaatttt gcgaaaatat catttctgtc ccactccctt
     2761 atgcatgagt tttactcatg taattttatt tttaaggaca tattacatct ggctaatgtg
     2821 taagagccac tacataataa atcattagtg gttctttatt atttctatct cactccctct
     2881 aaacaagaat aaat
//
LOCUS       DAKTLH010000207         2888 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.213, whole genome shotgun sequence.
ACCESSION   DAKTLH010000207 DAKTLH010000000
VERSION     DAKTLH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.213"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            159..1394
                     /gene="arcA"
                     /locus_tag="O0394_002610"
     CDS             159..1394
                     /gene="arcA"
                     /locus_tag="O0394_002610"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCY0500655.1"
                     /translation="MVQGPIQVNSEIGKLKTVLLKRPGKELENLVPDHLSGLLFDDIP
                     YLKVAQEEHDKFAQTLRDEGIEVVYLEKLAAESITEPEVRENFINDILTESKKTILGH
                     ETEIKEFFSKLSDQELVNKIMAGVRKEEIQLETTHLVEYMDDRYPFYLDPMPNLYFTR
                     DPQASIGRGMTINRMYWRARRRESIFMTYILKHHPRFKDKDVPVWLDRNSPFNIEGGD
                     ELVLSKDVLAIGISERTSAQAIEKLARNIFKDANTSFKKIVAIEIPNTRTFMHLDTVL
                     TMIDYDKFTVHAAIFKEENNMNIFTIEQNDGKDDIKITRSSKLRETLAEVLEVEKVDF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNQLLRDKGIKVIEITGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            1480..>2888
                     /gene="arcD"
                     /locus_tag="O0394_002611"
     CDS             1480..>2888
                     /gene="arcD"
                     /locus_tag="O0394_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCY0500656.1"
                     /translation="MDENKLGKTSLIGLVIGSMIGGGAFNIISDMGGQAGGLAIIIGW
                     IITAIGMISLAFVFQNLTNERPDLDGGIYSYAQTGFGDFIGFSSAWGYWFAAFLGNVA
                     YATLLMSAVGNFFPIFKGGNTLPSIIIASILLWGVHFLILRGVETAAFINSIVTVAKL
                     IPIFLVIICMIVVFNFSTFKSGFYGMTSGSVGVFSWGDTMAQVKSTMLVTVWVFTGIE
                     GAVVFSGRAKSKKDVGTATVIGLISVLVIYFLMTVLAQGVIQQNQISKLANPSMAQVL
                     EHIVGHWGSVLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNKAPV
                     NALIITNILVQLFLISMLFTDSAYQFAFSLASSAILIPYTLSAFYQVKYTIQNKSKAN
                     LKQWIIGIIASIYTIWLVYAAGLDYLLLTMLLYIPGLLVYSYVQRDNNKHLTKLDYTL
                     FIFIIVLAIIGIVRLITGN"
BASE COUNT         1003 a          403 c          544 g          938 t
ORIGIN      
        1 actattatat agtacaaaat acatattcat acaaaatttt gcattgaata aatctatata
       61 tgtgaaaacc ctccctaaac aattgtgaat taaatcacac gatgtttttt gaaagcgttt
      121 tataatgtga atgtaataaa gcaaaggagg aatttgaaat ggtacaagga cccattcaag
      181 taaatagtga aataggcaaa ttgaaaactg tgttgttaaa aagaccagga aaagaattag
      241 aaaatttagt acctgatcat ttaagtggtt tattattcga tgatattccc tacttaaaag
      301 ttgcacaaga agagcatgac aaatttgctc aaactttgag agatgaagga atcgaagtag
      361 tttatttaga aaaacttgca gcagaatcta ttactgagcc agaagtacgc gagaacttca
      421 taaacgacat attaacagaa tctaaaaaga caatattagg tcatgaaact gaaattaaag
      481 aattcttttc aaagttatct gaccaagaac ttgtaaataa aatcatggct ggcgtacgta
      541 aagaagaaat tcaacttgaa acaacccatt tagtagaata tatggatgat agatatccat
      601 tttacttaga tccaatgccc aacctttatt ttacaagaga tccccaagct tcaattggta
      661 gaggaatgac aattaacaga atgtattgga gagcacgacg tagagaatct atttttatga
      721 catatatact gaaacatcat ccaagattta aagataaaga tgtaccagta tggttagatc
      781 gtaactcacc atttaatatt gaaggtggag atgaattagt attatcgaaa gatgttttag
      841 ctattggtat atcagaacgt acatcagctc aagcaataga aaagttagca cgtaatattt
      901 tcaaagatgc aaacacaagt tttaaaaaaa tcgtagctat tgaaatacct aatacacgta
      961 catttatgca cctagataca gtactaacta tgattgacta cgataagttt acagtacatg
     1021 cagcaatatt taaagaagaa aataatatga atatatttac catagaacaa aatgatggta
     1081 aggacgatat aaaaattact cgttctagca agttacgtga aacacttgct gaagttttag
     1141 aagtagaaaa agtggacttt attccaacag gtaatggcga cgttattgat ggtgcacgtg
     1201 aacaatggaa tgatggctca aacacattat gtattcgacc aggggttgtg gtgacatacg
     1261 atcgcaacta tgtatcaaac caacttttac gcgacaaagg aattaaagtg attgaaatta
     1321 ctggtagtga acttgtacgt ggacgcggag gcccaagatg tatgagtcag ccgttattta
     1381 gagaagatat ttaaatttga aaggtggtaa aagtcacaag taattcattc attaaatcaa
     1441 ataacaagat ttttaccacc tttattaggg gagattaata tggatgaaaa taaattaggt
     1501 aaaacttcct taattggttt agtcataggc tctatgatag gcggtggtgc attcaatatc
     1561 atctcagata tgggtggcca agctggtgga cttgcaataa ttatcggttg gataataact
     1621 gctattggta tgatttctct tgctttcgta tttcaaaatt taacaaatga gcgaccagat
     1681 cttgatggag gaatttatag ttatgctcaa acagggtttg gagattttat tggtttttca
     1741 agtgcttggg gatattggtt tgcagcattt ctaggtaatg tggcttatgc aaccctatta
     1801 atgtcagctg tgggtaactt tttccctata tttaaaggag gtaacacact tccaagtatt
     1861 atcatagcat caattttatt atggggtgta cattttttaa tacttagagg tgtagaaact
     1921 gcagcgttta taaatagtat tgttacagta gctaaattaa tacctatatt tctagttatt
     1981 atatgcatga tagttgtatt caacttcagt acttttaaat ccggttttta tggtatgact
     2041 agtggaagtg ttggcgtttt tagttgggga gatacaatgg cacaagtaaa aagtactatg
     2101 ttagtaactg tatgggtatt cacagggatt gaaggagccg ttgtcttttc tggacgtgca
     2161 aagtctaaaa aggatgtagg aactgctacc gttattggtt tgatttctgt gctagtcatt
     2221 tatttcttaa tgactgtact agcccaaggt gtcattcagc agaaccaaat ttcaaaactt
     2281 gctaatccat caatggcaca agtattagaa catattgtag gtcattgggg ttcagtgtta
     2341 gttaatatag gcttaattat ctctgtttta ggagcttggt taggatggac attactagct
     2401 ggtgaattac cattcattgt agctaaagat ggacttttcc cgaaatggtt tgctaaagaa
     2461 aataagaata aagctccggt caacgcttta attattacta atatattagt tcagttattt
     2521 ttaattagta tgttgtttac agatagtgcc tatcagtttg cgttttcact tgcatcaagt
     2581 gcaatcttaa ttccatatac actcagtgct ttttaccagg ttaaatatac tattcaaaat
     2641 aaatctaaag ctaatttaaa acaatggata ataggaatta ttgcatctat ttacacaatt
     2701 tggttggttt atgcagctgg attagattat ttactattaa cgatgttgtt atatatacct
     2761 ggattactcg tatacagcta cgtacaaagg gataataaca aacatttgac aaaattggat
     2821 tatacgttat tcatattcat cattgtactt gcaataatag gaatagttcg tttgattaca
     2881 ggtaatat
//
LOCUS       DAKTLH010000208         2887 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.214, whole genome shotgun sequence.
ACCESSION   DAKTLH010000208 DAKTLH010000000
VERSION     DAKTLH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.214"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            311..1009
                     /locus_tag="O0394_002612"
     CDS             311..1009
                     /locus_tag="O0394_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HCY0500657.1"
                     /translation="MKLTTIAKATLALGILTTGVFTAESQTGHAKVELDETQRKYYIN
                     MLHQYYSEESFEPTNISVKSEDYYGSNVLNFKQRNKAFKVFLLGDDKNKYKEKTHGLD
                     VFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKVDAKNGFSINELFFIQK
                     EEVSLKELDFKIRKLLIEKYRLYKGTSDKGRIVINMKDEKKHEIDLSEKLSFERMFDV
                     MDSKQIKNIEVNLN"
     gene            1375..2058
                     /locus_tag="O0394_002613"
     CDS             1375..2058
                     /locus_tag="O0394_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HCY0500658.1"
                     /translation="MKFTALAKATLALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEA
                     LYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFF
                     VQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAFVKGYPYYIKKEKITL
                     KELDYKLRKHLIEKYGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQ
                     IKDIEVNLK"
     gene            complement(2143..2244)
                     /locus_tag="O0394_002614"
     CDS             complement(2143..2244)
                     /locus_tag="O0394_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500659.1"
                     /translation="MENFDVGGGNFTFMEIGIGFMTHKPKRLNDDID"
     gene            2322..>2887
                     /locus_tag="O0394_002615"
     CDS             2322..>2887
                     /locus_tag="O0394_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCY0500660.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPEDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFL"
BASE COUNT         1149 a          373 c          510 g          855 t
ORIGIN      
        1 tgtttttata ataaaaacat atcgaacatt gactacgtta ttaagctgct tttttgtaca
       61 ctttataacc aatagcttaa gatttaaaac taatcggaaa gaacaatgat tcaccaaaaa
      121 aatatttatg ttgctattaa aaatcagtta atacgaatgt taaaatacgt ttgattttca
      181 ttaataatga ttcaagttta tttaaatgag cgttaatgtc agtctgtttt gatgcacctt
      241 ataataaaga cagatagttc aaattacgta ataataacaa tccaatacat taagattgga
      301 gcaaaaaaat atgaaattaa caacgatagc taaagcaaca ttagcattag gaatattaac
      361 tacaggtgtg tttacagcag aaagtcaaac tggtcacgcg aaagtagaac ttgatgagac
      421 acaacgcaaa tattatatca atatgctaca tcaatactat tctgaagaaa gttttgaacc
      481 aacaaacatt agtgttaaaa gcgaagatta ctatggctct aacgttttaa actttaaaca
      541 acgaaataaa gcttttaaag tatttttact tggtgacgat aaaaataaat ataaagaaaa
      601 aacacatggc cttgatgtct ttgcagtacc tgaattaata gatataaaag gtggcatata
      661 tagcgttggc ggtataacaa agaaaaatgt gagatcagtg tttggatttg taagtaatcc
      721 aagtctacaa gttaaaaaag ttgatgctaa aaatggcttt tcgataaacg agttgttttt
      781 tattcaaaag gaagaagtat cattgaagga actggacttt aaaataagaa aactcttaat
      841 cgaaaaatat agattgtata aaggaacgtc tgataaaggt agaattgtta tcaatatgaa
      901 agacgaaaag aagcatgaaa ttgatttaag tgaaaaatta agttttgaac gtatgtttga
      961 tgtaatggat agtaagcaaa ttaaaaatat tgaagtgaat ttgaattagt tcgagttaat
     1021 agcataatag cttaagaagc ggcttaacga caaaatgtga attggcattt gtgtccttat
     1081 ataaggaact gtgttaaata cattactgtt gttaagttgt ttttgtaatt caaagagcag
     1141 aacagagtaa catcatcagt tgtagtaaac gataatccgg taaaacaact aaatgaaata
     1201 atgaaagtca tttaacctga acattaaaat atatttgttt ttcattaaga ataattcaag
     1261 tatatttaaa tcgaggttaa ttatcgtatg aaacgatgca cgttataata aaaatgtatg
     1321 attcaaatta cgtaatgaaa acaatccaat atattaagat tggagcaaat aaatatgaaa
     1381 tttacagcat tagcaaaagc gacattagct ttaggaattt taacaacagg aactttaaca
     1441 acagaagttc attcaggtca tgcaaaacaa aatcaaaagt cagtaaataa acatgacaag
     1501 gaagcattat accgatacta cactggaaag actatggaaa tgaaaaatat tagtgctttg
     1561 aaacatggta aaaacaactt acgttttaag tttagaggta ttaagattca agttttactg
     1621 cctggaaatg ataaaagtaa atttcaacag cgtagttatg aggggttaga tgttttcttt
     1681 gttcaagaaa aaagagataa gcacgatata ttttatactg ttggtggtgt aatacagaat
     1741 aataaaacat ctggagttgt cagtgcacca atattaaata tttcaaaaga aaagggtgaa
     1801 gatgcttttg tgaaaggtta cccttattac attaaaaaag aaaaaataac actaaaagaa
     1861 ctggattata agttgagaaa gcatctaatt gaaaaatacg gactttataa aacaatctca
     1921 aaagatggta gggtcaaaat tagcttgaaa gatggcagtt tttataacct tgatttaaga
     1981 tctaaattaa aatttaaata tatgggggaa gtcatagaaa gcaaacaaat taaagatatt
     2041 gaagttaact taaagtaaat cattacgaat aattaaaagt aattgaagcg gcttaacggt
     2101 gaaatgtaaa ttggtgcgca tagcttatac aaaaaggatg catcaatcga tatcgtcgtt
     2161 aagccgtttt ggtttgtgtg tcatgaatcc tatcccaatc tccataaagg taaaatttcc
     2221 accaccaaca tcaaaattct ccacatcgca acataaccaa atgttataat aaatctatta
     2281 cacaaagaga taaattactt attcaaaggc ggaggaatca catgtctatt actgaaaaac
     2341 aacgtcagca acaagctgaa ttacataaaa aattatggtc gattgcgaat gatttaagag
     2401 ggaatatgga tgcgagtgaa ttccgtaatt acattttagg cttgattttc tatcgcttct
     2461 tatctgaaaa agcggaacaa gaatatgcag atgccttgtc aggtgaagac atcacgtatc
     2521 aagaagcatg ggcagacgaa gaataccgtg aagacttaaa agcagaatta attgaccaag
     2581 tcggttactt cattgagcca gaagatttat tcagtgcgat gattcgtgaa attgaaacgc
     2641 aagatttcga tatcgaacac ctggcgacgg caattcgtaa agttgaaaca tcaacattag
     2701 gtgaagaaag tgaaaatgac tttatcggtc tgttcagcga tatggatttg agttcaacgc
     2761 gactaggtaa caatgtcaaa gaacgtactg ctttaatctc taaagtcatg gttaatcttg
     2821 acgacttacc attcgttcac agtgacatgg aaattgatat gttaggtgat gcatatgaat
     2881 tcctaat
//
LOCUS       DAKTLH010000209         2885 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.215, whole genome shotgun sequence.
ACCESSION   DAKTLH010000209 DAKTLH010000000
VERSION     DAKTLH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.215"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            186..686
                     /locus_tag="O0394_002616"
     CDS             186..686
                     /locus_tag="O0394_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0500661.1"
                     /translation="MTFEEKLSEIYNKIANEISGMIPVEWDQVFTIAYVNDRGGEIVF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEEDLEPWTSCEF
                     DFTRDGKLNVVFDYVDWMNSEFGPIAKENYYMYKKFGVLPETEYEINKVKEIDQYVKE
                     QDEAEL"
     gene            698..1198
                     /locus_tag="O0394_002617"
     CDS             698..1198
                     /locus_tag="O0394_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0500662.1"
                     /translation="MTFEEKLSQMYNKIASEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKPGSDELNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKEEELEPWTSCEF
                     DFTRDGKLNVSFDYIDWVNSEFGPMGREHYYMYKKFGIWPEKEYAINWVEKIKDYVKE
                     QDEAEL"
     gene            1209..1709
                     /locus_tag="O0394_002618"
     CDS             1209..1709
                     /locus_tag="O0394_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HCY0500663.1"
                     /translation="MTFEGKLSQMYNEIANEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKPDSDELNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKEEGLEPWTSCEF
                     DFTRDGKLNVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEYEMEEVKEIEQYIKE
                     QEEAEQ"
     gene            1720..2220
                     /locus_tag="O0394_002619"
     CDS             1720..2220
                     /locus_tag="O0394_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0500664.1"
                     /translation="MTFEEKISKLYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKPGSDDLNYYTDIPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEGLEPWTSCEF
                     DFTSEGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPEMEYEMEEIKEIDQYIKE
                     QDEAEI"
     gene            2231..2719
                     /locus_tag="O0394_002620"
     CDS             2231..2719
                     /locus_tag="O0394_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HCY0500665.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPVEWEKVYTIAYVDDEGGEVVF
                     NYTKPGSEDLNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKKEDLEPWTSCEF
                     DFTRKGNLKVSFDYIDWIKLGFGPSGKENYYMYKKFGILPDMEYEMEEIRAVEKYVKE
                     QE"
BASE COUNT         1135 a          280 c          592 g          878 t
ORIGIN      
        1 aaattattta gaattccagt tatcgtaaca atagttgcag ttattttagt tccattagtg
       61 tatttaggtt taaaatgagt aatattaatt tgaagaagca ttaagcgacg tagaggtgtt
      121 gtttagtgct ttttatttaa cggaaatgaa accaatatct attttgaaaa tataggggcg
      181 ataacatgac tttcgaagaa aaactaagtg aaatatacaa caagattgcg aatgagatta
      241 gtgggatgat accagtagaa tgggaccaag tatttacgat agcttatgta aatgatagag
      301 gtggagagat cgtttttaat tatactaaac caggtagcga tgaattgaat tattacacat
      361 atatccctag agagtataat gtctctgaaa aagtatttta tgatttgtgg acggatttat
      421 atagattgtt taagaagtta agaaacgcat ttaaagaaga agatttagaa ccatggacat
      481 catgtgaatt tgactttaca agagatggta aattgaatgt tgtatttgat tatgttgatt
      541 ggatgaattc agaatttggt ccaatagcaa aagaaaatta ttatatgtac aaaaagtttg
      601 gtgttttacc agaaacggaa tatgaaatta ataaagttaa agaaatcgat caatatgtta
      661 aagagcaaga tgaagctgaa ctataggggg agataacatg actttcgaag agaaactaag
      721 tcaaatgtac aataaaattg caagtgagat cagtggaatg ataccagtag aatgggagca
      781 agtatttaca atagcttatg taactgatca agctggggaa gtcattttta attatactaa
      841 accaggtagt gatgaattaa attattattc agacatacct aaagattgca atgtctcaaa
      901 agatattttt aagaattcat ggtttaaagt ttatcgaatg tttgatgagt taagagaaac
      961 ttttaaagaa gaagagcttg aaccatggac atcatgcgaa tttgacttta caagagatgg
     1021 caaattgaat gtatcttttg attatattga ttgggtgaat tcagaatttg gaccaatggg
     1081 aagagaacat tattatatgt ataaaaaatt tggaatttgg cctgaaaaag aatatgccat
     1141 aaattgggtt gaaaaaataa aagattatgt taaagagcaa gatgaagctg aactataggg
     1201 gagataatat gactttcgaa ggaaaactaa gtcaaatgta caacgagatt gcgaatgaga
     1261 ttagtgggat gataccggta gaatgggagc aagtatttac aatagcttat gtaactgatc
     1321 aagctggaga agtcatcttt aattatacta aaccagatag tgatgaatta aattattatt
     1381 cagacatacc taaagattgc aatgtctcaa aagatatttt taagaattca tggtttaaag
     1441 tttatcgaat gtttgatgag ttaagagaaa cttttaaaga agaagggctt gaaccatgga
     1501 catcatgcga atttgacttt acaagagatg gcaaattgaa tgtatctttt gattatatag
     1561 attggataaa tacagagttt gatcaattgg gccgtcaaaa ttattatatg tacaaaaaat
     1621 ttggggttat accagaaatg gaatatgaaa tggaagaagt taaagaaatc gaacaatata
     1681 ttaaagagca agaagaagct gaacaataga ggtgataaca tgactttcga agagaaaata
     1741 agcaaattat ataatgagat tgcgaatgag attagcagta tgataccggt agagtgggaa
     1801 aaagtatata caatggctta tatagatgat ggaggaggtg aagtattctt taattatact
     1861 aaaccaggta gtgatgactt gaattattac accgatatac ctaaggagta taacatctct
     1921 gtgcaagtat ttgatgattt atggatggat ttatatgatt tgtttgagga attaagagat
     1981 ttatttaaag aagaaggact tgaaccatgg acatcatgtg aatttgactt tacaagcgaa
     2041 ggtaaattaa aagtttcatt tgattatata gattggataa atacagagtt tgatcaatta
     2101 ggccgtgaaa attattatat gtataaaaaa tttggggttt taccagaaat ggaatatgaa
     2161 atggaagaaa ttaaagaaat cgatcaatat attaaagagc aagatgaagc tgaaatatag
     2221 gggagataac atgactttcg aagaaaagct aagtcaaatg tacaatgaaa ttgcaaatga
     2281 aatcagtgga atgataccag tagaatggga aaaagtatat acaattgcct acgtagatga
     2341 tgaaggtgga gaggttgttt ttaattatac taaaccagga agtgaagatt tgaattatta
     2401 ttcagatatt cctaaagatt gcaatgtctc aaaagatatt tttaagaatt catggtttaa
     2461 agtttatcga atgtttgatg agttaagaga aacttttaaa aaagaagatt tagaaccgtg
     2521 gacatcatgt gaatttgact ttacaagaaa gggaaattta aaagtatcat ttgattatat
     2581 agattggatt aaattaggtt ttggcccatc aggaaaggaa aactactata tgtacaaaaa
     2641 atttggtatt ttaccagata tggaatatga aatggaagaa attcgagcag tagagaagta
     2701 tgttaaagag caagagtagc agacatgtta taaaagactg tgcaaaatca ccctcgtttt
     2761 acatttgatt caaagaagaa ggtaaaagat aagattattt gcaacttaaa aagtcaatta
     2821 gcttatcggt atccatacat catggataaa tgagttcaac taattaacaa atcacgatat
     2881 aaatt
//
LOCUS       DAKTLH010000210         2868 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.216, whole genome shotgun sequence.
ACCESSION   DAKTLH010000210 DAKTLH010000000
VERSION     DAKTLH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.216"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..535
                     /locus_tag="O0394_002621"
     CDS             <1..535
                     /locus_tag="O0394_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0500666.1"
                     /translation="VTLLTLNNVLMVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLI
                     SHIAFCIPIVVIIVLPQLYEMNNNMLNAARDLGATEPQLLSNIIIPNILPSIIGGFFM
                     ALTYSLDDFTVSFFVTGNGFSVLSVEVYAMARKGISMEINAISTLLFAVIVLGILGYY
                     LIQYVINKKKLIKRGVK"
     gene            535..1608
                     /locus_tag="O0394_002622"
     CDS             535..1608
                     /locus_tag="O0394_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0500667.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVPNIKNLDSDYMNMSFDRGNKYSLPYFFGTVGILYNKEKYPNESFDSWKS
                     LYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVG
                     DEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKE
                     GAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVY
                     KDLGPEVLSEYNENFLNFKMSLK"
     gene            1682..2701
                     /locus_tag="O0394_002623"
     CDS             1682..2701
                     /locus_tag="O0394_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCY0500668.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
BASE COUNT         1047 a          379 c          515 g          927 t
ORIGIN      
        1 agtaacttta ctaacattga ataatgtctt gatggtatca tccgacgttg tcataggtgc
       61 atcattctta attatgttta caacgattgg ccattttact ggtcttggtt taggattttg
      121 gacagttcta atatctcata ttgcattttg catacctata gttgtgatta tcgtcttacc
      181 acaactgtat gaaatgaata ataatatgtt aaatgctgca agagatttag gagcgactga
      241 accacaatta ttaagcaaca ttattattcc taatatttta ccttctatta taggaggatt
      301 ctttatggct ttaacttatt cactagacga tttcacagta agtttcttcg ttactggtaa
      361 tggctttagt gtgttatcag ttgaagttta tgctatggcg agaaaaggaa ttagtatgga
      421 aattaatgcg atttcaacat tattgtttgc tgttattgta ttaggaatac taggatatta
      481 tttgattcaa tacgtgataa ataagaaaaa actaatcaag cgaggtgtaa aataatgaaa
      541 cgatttttac aactcattat aggtgcatta gttgtgggta tgctttgtct tactttaagt
      601 cattggttta aatctaaaga acaagtgcat acaaatcaaa aaatttacgt atacaattgg
      661 ggcgaatata ttgatccaga gttaattaag aaatttgaaa aagaaactgg cattcaagtc
      721 gtttatgaaa ctttcgattc aaatgaagcg atggaagcca aaattcgcaa tggcggtaca
      781 cattatgatg ttgcttttcc tagtgaatat acagttcaaa aattgaaaag agatcattta
      841 ttgttaccaa tagatcataa taaggtacct aatattaaaa atttagattc agattatatg
      901 aatatgtcat ttgatagagg caataaatat tctttacctt atttctttgg aactgtaggt
      961 attttatata ataaagaaaa gtatccaaat gaatcatttg atagttggaa gtcattgtat
     1021 aatcctaaat ttaaaaacca aattttacta gttgacggtg ctagagagat tataggcatg
     1081 agtttgaata aacttgggta taatcttaat gaccgtaatt cgcaccattt aaaagaagca
     1141 gagcgagatt taaccaaact agcaccacaa gtaagaggtg tcgtaggtga tgaaattacc
     1201 atgatgcttc aacaaaatga aggtaacata gcggttgttt ggagtggtgt tgcagcacct
     1261 ctagtgcaag aaggggataa atataattat gttattccta aagaaggatc gaatttatgg
     1321 ttcgacaata tggtaattcc aaaaacggca caaaataaag agggtgcata taaatttatg
     1381 aattttttat tagatgctaa aaataacaag caaaatacag aattcgtagg ctatgcaacg
     1441 ccaaacaagg ctgctcgaca attgttgcct aaagagatta aagacgacca tcgtttttat
     1501 ccgactaaga aagagcaaga acgccttgaa gtttataaag atttaggacc tgaagtttta
     1561 agtgaataca atgaaaactt tttgaatttc aaaatgtcat taaaataaaa tcataaatag
     1621 tagttattta ctgtatattt agttataata acgggataag tactataagg agttgtaacg
     1681 aatgactgga gaacaattta ctcaaattaa acgtccagta agtagattaa ctgaaaaagt
     1741 tctaggttgg ttatgttggg tgatgttatt agtgcttact gtcattacga tgtttatagc
     1801 acttgtttca ttcagtaata acacatcgat tgctaatctt gaaaatacat taaacaataa
     1861 tgcatttatc cagcaattat tagctggaaa tggctataat acaacacaat ttgtaatatg
     1921 gttacaaaat ggtatatggg ctattatcgt ttactttatt gtttgtttgt tgatttcatt
     1981 tttagctctc atatctatga atataagaat cttgtcaggt ttcttattct taatatcagc
     2041 aattgtaacg attcctttag ttttacttat cgttacttta atcattccga tattattctt
     2101 tattattgcg atgatgctat ttataagaaa agataaagtt gaaatggttg cgccacaata
     2161 ttatgaagag tataacggac cgatttatga ttatcgtgaa cctgtgtatg agcgccccca
     2221 accgaaagat gattattatg atgtgcctaa atatgaaaaa gaattggata aatcaaatac
     2281 tgtatatgat caagaacagg aaagagacaa atatgatcaa tttcctaaac gtgcagttga
     2341 aagtgaatat aatcatgatg agcgcactga ggaagaacca tcagtattat ccagacaggc
     2401 taaatacaaa caaaaaagta ctgaagaact aggtattgaa gatgatggtt attatgcaga
     2461 acctgaagtt gatccaaaag aattgaaagc acaacaaaag cgagaaaaag ctgaaattaa
     2521 agctaagaaa aaagaaaaac gtaaagctta taaccaacga atgaaagaac gtaggaaaaa
     2581 tcaacctagc gcagttagtc aacgtcgaat gaattttgaa gagcgacgtc aaatttacaa
     2641 caatgatatt tctgaagaac gcaattcaag tgaagttaag gacaaaaaag agcaagaata
     2701 aatattgatg atacccagat tagtaagcag aggtcttttg cttattaatc tgggttttta
     2761 tatgaggtta attatcgata acgtttaatt aaagtgttta ggtgtcataa ttttaaatga
     2821 cgatttctca ttactataca cctaaattat catcaatctg aattcaga
//
LOCUS       DAKTLH010000211         2852 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.217, whole genome shotgun sequence.
ACCESSION   DAKTLH010000211 DAKTLH010000000
VERSION     DAKTLH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.217"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            94..465
                     /locus_tag="O0394_002624"
     CDS             94..465
                     /locus_tag="O0394_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCY0500669.1"
                     /translation="MLKKWLGMALITPILTFIIWLFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
     gene            complement(520..1764)
                     /gene="fabF"
                     /locus_tag="O0394_002625"
     CDS             complement(520..1764)
                     /gene="fabF"
                     /locus_tag="O0394_002625"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCY0500670.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVNS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(1776..2717)
                     /locus_tag="O0394_002626"
     CDS             complement(1776..2717)
                     /locus_tag="O0394_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCY0500671.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASLKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSDGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKIKDDDTIVLVGFGGGLTWGAMTIKWGK"
BASE COUNT          866 a          573 c          420 g          993 t
ORIGIN      
        1 tcaataaagc tgaagaaatt aaaaaatcat tcctcaatgt ctgattttaa tgtaaactat
       61 gcgtaagagt tttctataag tgggagtgat caaatgttga aaaaatggct tggaatggca
      121 ctaataacgc caatattaac gtttattata tggcttttta atagccatac tatcattaca
      181 tatttaaata tattgtttta tgtttcatta attattttta ttagcatatt tttgatttta
      241 cttgtacaag aaggcatttt tgatgcgaca agttatggtt ttagaagatt aaagtatcaa
      301 atgtcatctt cgaaaaagaa gaagtctatc tctgatgatc catttttcaa cccacaagag
      361 gtaaaaaaag agcattactt tgtttctaca tggataatcc ccttattatt aataaatatt
      421 ttatatttta ttatgacgat tgtgctgtcg ctcatcttag tataaagaaa ctgaaaaaat
      481 cttcagtaac taaaacaacg ttactgaaga tttttatagt tatgcttcaa atttcttgaa
      541 tactaatact gcgttatgtc caccgaatcc taagctatta ctcattgcat aagtaatatc
      601 aaggtcttgc gcttcatttg gaacaatatc caaatcacat tctggatctg gtgttaccgc
      661 atgaattgtc ggtgcgactt tagagtcttt aattgaaagc gctgagaaga ttgcttcaat
      721 tccacctgtt gcaccaagta agtgaccagt cattgatttt gttgagttaa cttttaagtg
      781 tttagctgct tcaccaaatg tatttttaat agctttaact tcatttaagt caccaacagg
      841 agtacttgta ccatgggcat ttaagtattg tacatcttta ggttcaatac cagcatcatc
      901 catagctgct tgcattgctc tagaaccacc ttcaccttct ggagctggcg ctgtaatatg
      961 ataagcatca cctgtagtac catagccaac tatctcagca taaatattgg cacctcgagc
     1021 ttgtgctgat tctaaagatt caattactaa aataccagca ccttcaccca taacaaaacc
     1081 atctctacct tcttggaatg gacgacatgc tgtttcaatg tcatcatttg tagaaagcgc
     1141 tcgacttgca ctgaaaccag caattgccat atgagtaatt ggtgcttctg taccaccagt
     1201 aatcattgca tctgcatcac cgcgttgcac aattttaaat gcttctccga ttgaatttgt
     1261 acctgttgca catgctgtaa ctgttgcacc atttggtcct tttgcaccta agtcaattga
     1321 tacttgccca gttgccatat caggaattaa cattggtacg aaaaatggac tcacacgtct
     1381 tgggccttta tccattaatt gtttatgtgc aatttcaaat gtttccatac caccgatacc
     1441 agaaccaatc catacaccga ttcgatctgc agtattttca ttgatatcta attgcgcatc
     1501 tttaacagcc tctctagctg ctacaattgc atattgagta aatctatcca tacgacgcgc
     1561 ttcttttttg tcgatatgat cttcaatatt aaagttttta agttctcctg ctaagtgaac
     1621 gctataaggt tcagtatcga tacgtgtaat tttatcgata ccatttacgc cttttagagc
     1681 attctcccat gttgttttga catcattacc gattggagaa agggctccca tacctgtaat
     1741 aactactctt ttattttgac tcattcgtta tcctcctatt ttccccattt tattgtcatt
     1801 gcgccccaag ttaggccgcc accgaatccg acaagaacaa ttgtatcatc atctttgatt
     1861 ttaccatttt ttaattcttg atcgatactt aaaggtattg acgcagctga agtatttcca
     1921 tatttattta cagaaacact cattttgtct tttgaaatac ctaagcgttc tctagctgat
     1981 tccataattc taatattagc ttgatgagga ataaataaat ctatatcatc tgatgttaaa
     2041 ttcgcttttt caactacacg tgttgatgca tcacccataa ttctaacagc aaatttaaat
     2101 acttctcgac cattcatttt cagtttacca gtatctttat ctaaatataa atgtttacca
     2161 cctgtaccat cagaacccat ttcataactt ataatacctc tgccatctga aacttcaccg
     2221 atgataaccg cacctgcacc atctccaaat agaactgcag tagaacggtc agttaaatct
     2281 gttattttag ataatttatc tgcaccgaca actaaaatgt tatgataatc tccagattga
     2341 acatattgtt tagctgtaat cattgaatac ataaatccag aacatgctgc aagttgatcc
     2401 atagaggcaa ctttgcccgt ccctaaacgt tcttgcaaca tatttgcgac agttggaaat
     2461 ggcatatctc cagttgctgt ggcaacaatt atcatatcta tatcttcggg ctgaatacca
     2521 gcgtcagcga ttgcttttaa acttgcttca tatgctaaat ctgaagtatc ttgatcatca
     2581 tctgcccaat gtctttcttt aattccagtc atcttagaaa tccattcatc agatgtatct
     2641 aaaaattgct caaaataggc attgtcaata atcttttctg gcgcatatgc accaaaacct
     2701 ttaataccca cgttcatagt tgaacacctt cttaataaaa tttttaatac caggtattaa
     2761 tttaacatag attacttttt aaactcaagt cagaggtagg aatattgact agttaataaa
     2821 attttaatgc tttattatcg tcaaatttca gt
//
LOCUS       DAKTLH010000212         2802 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.218, whole genome shotgun sequence.
ACCESSION   DAKTLH010000212 DAKTLH010000000
VERSION     DAKTLH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.218"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            8..1084
                     /locus_tag="O0394_002627"
     CDS             8..1084
                     /locus_tag="O0394_002627"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCY0500672.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
     gene            1334..2017
                     /locus_tag="O0394_002628"
     CDS             1334..2017
                     /locus_tag="O0394_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0500673.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(2133..2795)
                     /locus_tag="O0394_002629"
     CDS             complement(2133..2795)
                     /locus_tag="O0394_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCY0500674.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMINKIARIIQTYRQVD
                     TR"
BASE COUNT          984 a          392 c          529 g          897 t
ORIGIN      
        1 aatcaccatg tcacaagcag ttaaagttga acgacgagaa acattaaaac aaaaaccaaa
       61 tacatctcaa ctaggttttg gtaaatattt tactgattat atgttgagtt atgattatga
      121 tgcagataaa ggatggcatg atttgaagat agtaccttat ggtcctattg aaatttcacc
      181 tgctgcacaa ggtgttcatt atggtcaatc ggtattcgaa ggattaaaag catataaaag
      241 agatggggaa gttgcacttt tccgtcctga agaaaatttt aagcgtctta ataactcgtt
      301 agcacgatta gaaatgcctc aagtagacga agcagaattg ttagaggggc taaaacaatt
      361 agttgatatt gaaagagatt ggattcctga aggggaaggt caatcattat atattcgtcc
      421 atttgttttt gcaacagaag gggcacttgg cgttggtgca tcacatcagt ataaattatt
      481 aattatttta tctccttcag gtgcatatta tggtggtgaa actttaaaac caactaaaat
      541 ctatgtagaa gatgaatatg tgcgtgctgt tcgtggcggt gtaggctttg caaaagttgc
      601 aggtaactat gcggcaagtt tattagcaca aactaatgca aataaattag gttatgacca
      661 agtattatgg cttgatggtg ttgaacagaa atatatcgaa gaagttggta gcatgaacat
      721 tttcttcgtt gaaaatggca aagtaattac accagagttg aatggcagta ttttacctgg
      781 tattacacgt aaatctatta tcgaattagc taaaaactta ggatatgaag tcgaagagcg
      841 ccgcgtttca atcgatgaat tattcgaatc atatgataaa ggtgagttaa cagaagtatt
      901 tggtagtggt actgcagcag ttatttcacc tgtgggtaca ttgagatacg aagatcgtga
      961 aatcgttatt aataataatg agactggtga aattactcaa aaattatacg acgtctatac
     1021 tggtattcaa aatggtactt tagaagataa aaatggttgg agagtcgttg taccaaaata
     1081 ttaataaaaa ttgaatataa tcatgaaaac aatatgcaaa agttaatcat gactaaaatc
     1141 cctttcaatg aagcgaagca tggtaataaa ttaagtttac aatgtttatt gttaagtatt
     1201 gaaggggatt tcacttatta ttatatttaa ttcaatattt aaatagaaca atactacgag
     1261 ttcattttca ggggataacg aatatcatag aatcagcata atacgaaata aataaatagg
     1321 agtattgata tcaatggaat ggatattatt tgataaagat ggtacgttaa ttgaatttga
     1381 tagaagttgg gaaaaaatag gggtacgatt tgtacaatca ttgcttgaga ctttcccagt
     1441 acataataaa gaagctgctt taagacaact cggtgtcatt aaagaatcta ttgatccaaa
     1501 atcagtgatg ggttcaggat ctttacaaca aattatccag gcatttaatg atgtgacggg
     1561 acaagataca accgactggt ccaagtcaac aagtcaaaag ctggtagatg aacgtattcc
     1621 tgaaattaat tgggtagaag gtgttaaaga agcacttatc gatttgaaag caaaaggcta
     1681 tcaacttggt attgttacga gtgatactaa aaaaggtgta gaacaatttt tagcacatac
     1741 caatgctacc tcgttgttcg atttgatcat ttctaccgaa gcggatgcct atgagaagcc
     1801 aaatcctaaa gtattatcgc ctttatttga gcaatataat gtagatcctc agaaagtagc
     1861 tatagtagga gacactgcta atgatatgaa gacagcaagt aatgcaaatt taggtatggc
     1921 aataggtgta ttaacaggta ttgcaacaaa agaagaatta catgaagctg atattatttt
     1981 aaatagtgcg gcagatattt tagaagcttt aaattaaaaa gaaagacata gtgtatatgt
     2041 gaattaatgt attcgataga attgataaaa taatcgataa tatctatgga atatacattg
     2101 aatataaaca tatacgctat gtctttagtc ttttatcgtg tatctacttg tcgatatgtt
     2161 tgaataattc gagcaatttt gtttatcata ggatttaaag attcggggtc cttatggata
     2221 tcatattcat taatattgat acgtacaact ggacatgcat taaagctatt aatccaatcg
     2281 tcatagcgtt taaatagctt tttccagtat tcagggtctg tattaatttc catttcgcga
     2341 ccacgttcaa taatacgatc aatgacctca tcatagttac attctaaata aatcattaca
     2401 tcaggtttag gaaaataagg tgtcatgacc atggcattaa ataagtctga atatgttttg
     2461 aaatcttctt tactcattgt gccttcttct tcatgcattt ttgcaaaaat atcaacatct
     2521 tcataaattg atcgatcttg gacaaagcca ccaccatatt caaacatacg cttttgttct
     2581 ttaaaacgtt cagctaagaa gtaaatttgc aaatggaaac tccatcgttc aaaatcgctg
     2641 taaaatttat ctaaatatgg attatgttcg acattttcaa aagacgtttt aaagtttaat
     2701 ttatctgcaa gtgcttgcgt tagtgttgat tttccaacac caactgtacc tgcaatggtt
     2761 ataatggcat tttgtggaat accgtaatta ttcattggta at
//
LOCUS       DAKTLH010000213         2779 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.219, whole genome shotgun sequence.
ACCESSION   DAKTLH010000213 DAKTLH010000000
VERSION     DAKTLH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.219"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(95..343)
                     /locus_tag="O0394_002630"
     CDS             complement(95..343)
                     /locus_tag="O0394_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCY0500675.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            451..1404
                     /locus_tag="O0394_002631"
     CDS             451..1404
                     /locus_tag="O0394_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0500676.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTK
                     QHVFHFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTKLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVELEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            1394..2773
                     /locus_tag="O0394_002632"
     CDS             1394..2773
                     /locus_tag="O0394_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCY0500677.1"
                     /translation="MLHDILRNKFGFESFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQAVLTTLQSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFD
                     EKLQNLFLR"
BASE COUNT         1011 a          444 c          439 g          885 t
ORIGIN      
        1 caaaataata tacacataat aataaaaagc aggtgactat caataaacga tagcttgcct
       61 gctttttcta tagaacattt gtctaaaaaa tcaattattc aaattttaaa gcgtccccat
      121 caaatgattc gtctgcaatt ttaatagaat ctgtagggca tccatcaatt gcatcttcca
      181 tatcttcata taattcctca ggtacttctg cagtaccttg gttatcgtca aggattacga
      241 aagcaatacc ttcgtcgtcg taatcatata tatctggcgc tgctgcaccg catgcaccac
      301 atgcaataca agtatccata tcaacgattg tatattttgc caatgtcttc gcctcctttg
      361 ataaaaatgc taaaatagta atgtgactaa aattttagac agcatcattt ttattttcaa
      421 attatccgtt ttacagagtg agggttaaat ttgcaacaca ttataaaaac agcattacaa
      481 caaacattta actataaaac aaataaaagt atttacaata tcttagttgg taagaaatct
      541 caccaaacct tttttgacgc ttgtagtcaa caacagttgt cattatatca cagtttacca
      601 ctattaaaat atccgtcttt tgagctattt ttagaaaaaa tcaatgaatt taatgctgaa
      661 atggaaatca tgttgcatcc tagatataca tttgaaagca tggggcaaac atttcaagca
      721 attcaactat tagtgcaaac catgtctaat accaaacaac atgtttttca ttttgtacca
      781 atctctcaaa ataataagat acaagaaact gtaaagattg tctataacta cattaaagaa
      841 aaccaattac aaattgattt tgaaaatgaa ctacataact tgtttaaagc aataacttta
      901 aaagggccat gttacttaca ttattatttg caaggctatg atgaaccaat gtatacgaga
      961 cagcaagtta gtttaataga aaagctatct caacagcaat tgtttgaata cgaaatgaat
     1021 aatttagtga caatgatgtt tgaattagaa agtggagaat atactatttt atcaaaaata
     1081 ataatgaaac ctacattatt aaatcaaact tatattactt atacaaaatt gcttgaacaa
     1141 ttcacgatgg aagatatagc ggctcaacaa caagttaaaa tcaatactat cgaagatcat
     1201 gtacttgaaa tcttaatcaa aggttacatg tctaattacg atgattatgt tgaactagaa
     1261 gatcaactcc agtttttgaa tttttatcaa cagcatcgtg gcgaacgatt aaaattttac
     1321 aaagaacaat ttgacacgtt atcatatttt caattaaaag tattaatcgt tggatttgaa
     1381 agaggtgatc tgaatgttgc atgatatttt acgaaacaaa tttggattcg agagttttaa
     1441 accgggacaa caggaaatta tagaaagtat aatgtctcaa caacacactc taggtatact
     1501 tccaactgga agtggaaaga gtttgtgtta tcaaatacct acgtatttat caggtaagcc
     1561 gacattaatt atctcaccgt taatatcttt aatggatgac caagttatgc agttgaaaat
     1621 aaatggagaa aaacgtgtaa catgtattca ctctggtatg gatgaaattg agaaaaagca
     1681 taatattaaa tgtttacgac atagccgctt catctttcta agtccagaat ttctcctgca
     1741 accgtcaaat tttaaattaa tatctatgat agactttggc atgattgttc tagatgaagc
     1801 acattgccta tctgaatggg gatatgattt cagaccacat tatgctctaa taggaaaagt
     1861 aacaaagcat tttaaagaag cggttgtctt agcattgaca gcaactgcac caccgcattt
     1921 acaagatgat ttgacggaaa tgttagcgat tcaattcaat gttattaaaa ctacaatgaa
     1981 tcgcccaaat ataagcttta agcatcttaa ttttcatgat gatgaagata aaattgaatg
     2041 gttgctgccg tttctacaac agtcgggacc aacgattatt tatgtctcat cgaaaaagat
     2101 gtgtctgaat ttagcgcaac ttatttatga ttcaggtttt cttacaggta tttatcatgg
     2161 tgatatgaat tatcaagagc gacacacagt tcaacaacaa tttttaaata atgatattcc
     2221 gattatagtc gcaacgagtg cttttggtat gggaattaat aaaaaagata ttcgcacaat
     2281 cattcacttt catctttcaa caagtccttc taactacatt caagaaattg gccgtgcggg
     2341 tcgcgatggt gaactaagtc aggcaattag tttattccaa ccggacgata aatatatttt
     2401 agaaacgtta ttatttgcag atatgataac agaagaagat gtacaaaatt tcgaaatagg
     2461 agaattttta gctcccgata aacaagccgt tttgacaacg ttgcaatcat tctatagtat
     2521 cggcgccttg aaacagatat ttaagcaatc atttaaacga aagcaattag gattctttcg
     2581 catgattggc tattgcaaat tggatcaatg tagacggaag tatttattag aatttttcgg
     2641 tgaatatcca ccggcacaag atcgatgttg tgacaatgat tctaatataa ctgatatcgc
     2701 aattttaaat aagaagaagg taattagaag tattggattt gatgaaaagt tgcaaaattt
     2761 atttctcaga tagtattac
//
LOCUS       DAKTLH010000214         2738 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.220, whole genome shotgun sequence.
ACCESSION   DAKTLH010000214 DAKTLH010000000
VERSION     DAKTLH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.220"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            196..837
                     /locus_tag="O0394_002633"
     CDS             196..837
                     /locus_tag="O0394_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCY0500678.1"
                     /translation="MEILLCEVRVVNKNPRYRIIKYKNDYLMIDLVSTWLVLFFPFIN
                     WLIPKKYVQISREDFDNLNIVKPVKNKALWPAIGSILLFGTMFRDKIYIPDSHLEKNC
                     VIIICSVLLLSILVFYIYLNQKVKLSIYNDRSSNGKIMIFPSFKNLCFVLFSYFFCGG
                     LSIMFLDVLISLSIQNIIVFIAWVIMTMLFFFINMSSIIDKKIHVIYLRSYKY"
     gene            925..1542
                     /locus_tag="O0394_002634"
     CDS             925..1542
                     /locus_tag="O0394_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0500679.1"
                     /translation="MLCETEIINKNPKYRVIKYDDEYLMVDVIRTWLVYFFPFINWFI
                     PKRCAKISREEYEKLNTVKPVKNKNFWPVVGGTILLGATSRKYIHLLNIQLEKRSVIF
                     ICFVVFLCILIFFVLLNRKLKLKVFDNKKEEQKIILVPTLKNAVLILYGYLLIGGMSI
                     LALSMLLTLENQNLITFIAWGMGLMLFFLMNITLIVNKTVKVIKR"
     gene            1617..2246
                     /locus_tag="O0394_002635"
     CDS             1617..2246
                     /locus_tag="O0394_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCY0500680.1"
                     /translation="MLCESRVINQNPKYRIIKYNNEYFMVDLVSTWIAYFLPMINWFI
                     PKKYAKISREEFESLNIVKPAKNNTFWPVAGFAVLLTTLTRKYIYLLNIHLEKEIVIL
                     TCCMILLGVFALFIYINTKLKLHIFDKNKSNNEKIILIPTFKNICLSLFAYILFGGLS
                     TMALSMLVTSSPQNIIEFLALIGMTACFFLLNMSSVLDKKIHVILKTNK"
     gene            2303..2419
                     /locus_tag="O0394_002636"
     CDS             2303..2419
                     /locus_tag="O0394_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500681.1"
                     /translation="MILNRGAILEQGNKTAKVIKSELITNYIMLATLQIKNN"
     gene            2442..>2738
                     /locus_tag="O0394_002637"
     CDS             2442..>2738
                     /locus_tag="O0394_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0500682.1"
                     /translation="MLCESKIINKNPKYRIIKYNDEYLMIDIISTWISLFFPFINWFI
                     PKRYVKISREEFENLNIVKPAKKNVFWPVAGISTLFAVTLRKYTHLLDTQLDRKL"
BASE COUNT         1024 a          316 c          418 g          980 t
ORIGIN      
        1 ttttttcttt ctaataagca tgggctcaat tagtaataaa aaggtttatg ccaaacttaa
       61 aaagcaatag aaattactaa acttagattg tagttcgtaa gttaagtaat aaacaggaaa
      121 atagtatgtt actaaaatac aaaacactta taaagtatct gaaagtgtat tagatataaa
      181 accaacgaga cggaagtgga gatattgctt tgcgaggtta gagttgtaaa caaaaatccc
      241 agatatagaa ttattaaata taaaaacgac tatctgatga ttgatttagt aagcacctgg
      301 ttagttttgt tctttccgtt tattaattgg ttaataccaa aaaaatatgt ccaaatcagc
      361 agagaagatt ttgataattt aaatattgtc aaaccagtta aaaataaagc tttatggcca
      421 gctataggta gcatactttt atttggcact atgtttagag ataaaatata tatacctgat
      481 tctcatttag aaaaaaattg cgttattatc atttgttcgg ttttattatt aagtattcta
      541 gtgttttata tatatttaaa tcagaaagta aagttatcta tctataatga tcggagtagc
      601 aatggaaaaa ttatgatttt tccatcattc aagaatcttt gctttgtact attttcttat
      661 tttttctgtg gtggattatc aatcatgttc ttagatgttt tgattagttt atctatccaa
      721 aatattatag tatttattgc ttgggttatt atgacaatgc tatttttctt tataaatatg
      781 tcttcaataa tagacaaaaa gattcatgtt atatatttaa ggtcatataa atattaattt
      841 tgatacaaaa gggcacaagt gttttgagaa acagaaacga taatatagct gtgtgaattt
      901 gttattaata ggaagtgata attattgctt tgtgaaaccg aaattatcaa taaaaatccg
      961 aaatacagag ttattaagta tgatgatgag tatttaatgg tcgatgtaat aagaacttgg
     1021 cttgtatact tttttccatt tattaattgg ttcattccaa aaaggtgcgc caaaattagt
     1081 agagaagagt atgaaaaact gaacactgtt aagccagtta aaaataaaaa tttttggcct
     1141 gttgtaggag gaacaatcct actaggtgct acttccagaa agtacataca cttacttaat
     1201 attcaattag aaaaaagatc ggttatattt atatgcttcg ttgtatttct atgtatttta
     1261 atcttttttg tgcttctgaa tcgaaaatta aaattgaaag ttttcgataa taaaaaagaa
     1321 gaacaaaaaa taattttagt accaacatta aaaaatgctg ttcttatatt atatggctat
     1381 ttgttgattg gaggcatgtc aatattagct ttaagcatgt tgctgacatt agaaaaccag
     1441 aatttaataa cttttatagc ttggggtatg gggttaatgt tgtttttctt gatgaatata
     1501 acactcattg tcaataaaac agtcaaagtt ataaaaaggt gaataaataa ctaattatat
     1561 tgtattggcc gttcttcaaa taaaaaataa tttgaataag gtggaagtga taaacagtgc
     1621 tttgcgaatc tagagtcatt aatcaaaacc ctaaatatag aattattaaa tacaataatg
     1681 aatatttcat ggttgattta gtaagtactt ggattgctta ttttttgccc atgattaatt
     1741 ggtttattcc caaaaagtac gcgaaaatta gtagagaaga atttgaaagt ttaaatattg
     1801 tcaaacccgc taaaaataat actttctggc ctgttgcagg atttgcagtg ttattaacaa
     1861 ccttaacaag aaaatatatc tatttgctta acatccattt agaaaaagaa atagttatat
     1921 taacatgctg tatgatactt ctaggtgttt tcgcattgtt tatatatata aatacaaaat
     1981 tgaagttaca tatttttgat aaaaataaaa gtaataacga aaagatcata ttaataccta
     2041 catttaaaaa tatttgttta tccttatttg cttatatatt atttggtgga ttgtcaacaa
     2101 tggctctgag tatgttagta acttcatccc ctcaaaatat aatagaattt cttgctttaa
     2161 ttggcatgac tgcatgcttc tttctactga atatgtcatc ggttctagat aaaaaaattc
     2221 atgttatttt aaaaacaaat aagtagtaaa attgattaac ttaggtagta tcggatactt
     2281 aaatgttggt tcataaaaag caatgatttt aaatcgagga gctatcttag aacagggaaa
     2341 taaaacagcc aaagttataa aaagtgaatt aataactaat tatattatgt tagccacgct
     2401 tcaaataaaa aataattaga ataaggtggg attgataatc aatgctatgc gaatctaaaa
     2461 tcatcaataa aaaccccaaa tatagaatta ttaaatataa tgatgagtac ttaatgatcg
     2521 atataataag cacttggatt agtttatttt ttccttttat taattggttc atccccaaaa
     2581 gatacgtcaa aattagtaga gaagagtttg aaaatttaaa tattgtgaaa cctgctaaaa
     2641 agaatgtttt ttggccagtt gcaggtatct ctactttatt cgcagttaca ttaagaaagt
     2701 atacacattt acttgacact caacttgata gaaaatta
//
LOCUS       DAKTLH010000215         2732 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.221, whole genome shotgun sequence.
ACCESSION   DAKTLH010000215 DAKTLH010000000
VERSION     DAKTLH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.221"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            55..1317
                     /locus_tag="O0394_002638"
     CDS             55..1317
                     /locus_tag="O0394_002638"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCY0500683.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(1411..2685)
                     /locus_tag="O0394_002639"
     CDS             complement(1411..2685)
                     /locus_tag="O0394_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCY0500684.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
BASE COUNT          885 a          435 c          468 g          944 t
ORIGIN      
        1 ttaaagtatt ttaacaagta ttcaataaat aattataaat aggaggaaat aataatgacg
       61 aatgtattaa ttgaagattt aaaatggaga ggtcttattt atcaacaaac tgatgaacaa
      121 ggtattgaag atttattaaa taaagaacaa gtgacgttat actgcggtgc cgatccaacg
      181 gcagatagtt tacatattgg tcacttacta ccattcttaa cattaagacg ttttcaagaa
      241 catggacatc gtcctatcgt tttaattggc ggtggtacag gtatgattgg tgatccatca
      301 ggtaaatcag aagaacgtgt gctacaaaca gaagaacaag tagataaaaa tatcgaaggt
      361 attagtaagc aaatgcacaa tatttttgaa tttggaacag accatggtgc agtgcttgtt
      421 aataatagag actggttagg acaaatctca ttaattagtt ttttacgtga ctatggtaaa
      481 cacgtcggcg ttaattacat gttaggtaaa gattcaatcc aaagtcgttt agaacatggt
      541 atttcatata cagaattcac atacacgatt ttacaagcta ttgatttcgg tcatttgaat
      601 agagaattga attgtaagat tcaagtaggt ggatcagatc aatggggtaa tatcacaagt
      661 ggtattgaat taatgcgtcg tatgtatggt caaacagacg catacggttt aactattccg
      721 cttgtaacta aatcagatgg taagaaattt ggtaagtctg agtcaggtgc tgtttggtta
      781 gatgctgaaa aaacaagtcc ttatgaattt tatcaattct ggattaatca atcagacgaa
      841 gatgtaatta aattcttaaa atactttact ttcttaggaa aagaagaaat tgatcgctta
      901 gaacaatcta aaaatgaagc accgcattta cgtgaagctc aaaaaacatt agctgaagaa
      961 gtaactaaat ttattcatgg tgaagatgca ttaaatgatg caatccgtat ttcacaagca
     1021 ttatttagtg gtgatttaaa atcattatca gcgaaagaat taaaagatgg atttaaagat
     1081 gtgcctcaag tgacattatc aaatgacaca acaaatatcg ttgaagtcct tattgaaaca
     1141 ggcatttctc cttctaaacg acaagcacgt gaagatgtta acaatggtgc gatttatatt
     1201 aatggtgaga gacaacaaga tgttaattat gctttagcac cagaagataa aattgatggc
     1261 gaatttacga ttattcgtcg cggtaagaaa aaatacttca tggttaacta tcaataaata
     1321 taattgcata gctaaataaa ttagagccta ctcatattca ttcctaagaa tgtaatgagt
     1381 aggctcttaa tgtacttttc tgtctgtaaa ttatctaaag aaatctctat cgccttggcc
     1441 aggtgattga cgttctgatc ggctttgacg ttttggttgt tctttttgtt gttttaattt
     1501 cactttaact tcttttgttt taccatcacg gataacggta acagtgactg attcaccagg
     1561 ttttttattt tcatataaat agcttcttaa atcaacatca tctttaattt tcttgccatc
     1621 aatttctgta ataatatcac cttttttaag atcaatatca ctatcagctt tggcgacata
     1681 aataccgtct tctctatcag tatgaagttg ctcgcgctct tcttcaggaa tatctttcaa
     1741 attaattaaa ccaataccaa tcgaagggcg gtcaatttta ccatgtttta caagttgttc
     1801 aattgttact ttaacttcat tacttggaat agcaaacccg ataccttcaa cttgtgtcgc
     1861 agcaattttc attgagttaa taccaactaa attaccatta atatctacta atgcgccacc
     1921 tgagttacct gggttaatag cagcatctgt ttgaagaacg ctaacttttg tattgccacc
     1981 agttgtctca gcgtcaatcg tacgttcgct tgctgaaatg ataccagatg ttacagagtt
     2041 agcaaattgt aatcctaatg ggttacccat tgcgaatacg ctatcgccag tttgtacttt
     2101 tgaagagttg gcaaattgaa tcgctttaat accttttgta ttttcaattt taagtacagc
     2161 aatatcagtt actgcatctt taccaactaa tttcgcttta acttgttttt tattatgtaa
     2221 ttggactcta atttcatttg cgccatcaat aacatgattg tttgtaacga tataagctga
     2281 attgttgttt atttgataga taacacctga acctactcca gcttcagatg gtttagatga
     2341 tttgcctttt aataagtcgt ctacacttga tgctttttgc atgttaataa ctccaacaat
     2401 tgtaggggag acagatttta tcatttcatg aacggtaccg aatttcttgc tttgaccgtc
     2461 taattgattg ccacctttat tatttgttgt ctgaacagtt gaaccatctt tatttaaaat
     2521 tgtactattt aatactttgc ctataccaag tactagaagt gcaccaataa ttccagcaat
     2581 caatgcaacg atgactgttt taaaccatgg aaatttaggt cttctgtatc taggtgtttg
     2641 gctatggttt gttgtagaat gatctgtatg attaaaatct gacatactta acctccatta
     2701 tatgatttat atatgcttta attatgtctt tt
//
LOCUS       DAKTLH010000216         2713 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.222, whole genome shotgun sequence.
ACCESSION   DAKTLH010000216 DAKTLH010000000
VERSION     DAKTLH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.222"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            46..489
                     /locus_tag="O0394_002640"
     CDS             46..489
                     /locus_tag="O0394_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCY0500685.1"
                     /translation="MKIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLRRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            683..2281
                     /locus_tag="O0394_002641"
     CDS             683..2281
                     /locus_tag="O0394_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCY0500686.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDAEIEKWQQQKDSNQ"
     gene            complement(2341..2670)
                     /locus_tag="O0394_002642"
     CDS             complement(2341..2670)
                     /locus_tag="O0394_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500687.1"
                     /translation="MAYSILNLYSILIQYKCKINHKKDIQYLHPRKTLQLPLILKIIL
                     NLALIGIVIIVLTHTEFQLNAGDSNVFLLNWDIRGLTSYIFSFLILVALVSKLILIFI
                     TRRQRTS"
BASE COUNT          985 a          396 c          520 g          812 t
ORIGIN      
        1 taaagtaata tgaccaaaaa tataaatgaa atgaaggcat ttattatgaa aatttatgat
       61 gaatatagaa gttatgtaat agaagaactg gatgattgtc tcacaataca aaaaaataat
      121 gacactgcat attacgatgt gctagaagcg ataaatgatt tatcaaatga ttcattatgt
      181 gtgttaaatc atttatatat taacgaagga caagaagaaa cattcgagca aaaatttttg
      241 cgacgtaata agcatttaaa aaacgttgat ggatttaaag cattaaggtt tttaagacct
      301 agaacagctg gtagacacta tatcattatt acgctgtggg aaaataggca agcattttat
      361 cattggcaaa attcagctga gtacaagcat acacacaagc atcgtggcac ttcgaaaggt
      421 gcagatgtaa aaataattaa tcgtgaatta tcatacaata ttcgtattga attagctgat
      481 atggtctagc ccgagatatc aaccctcaat catttgaaag tccttgaatg ctacgtcatg
      541 tcaaagtgta gtgtcgagga ctttggtatt taaccctatt taattgaaaa ttctgtaaag
      601 ataggcgtat gatgtatata attcatttat aaaattacaa tgttcatcac aggaaaagtg
      661 gatgtatgaa aggaagttag ttatgcaaaa gtcagctcta ttcgcgcctg aaaaatataa
      721 cattatttcg gaaatcgaaa aatatagcca cataccagat aaaaaggcaa ttttatatca
      781 taatactgaa ggcgaagata tatcagtcac ttaccaacag ctcattgaac agtctaataa
      841 agtaggtaat gttttagcat cgcacggttt atctaaaggc gataaagtac tcattatgat
      901 gccacgatct attgcgacgt atgaattata tattgctgct ttaaaattag gtgttgcaat
      961 cattccatgt tctgaaatgc taagaactaa agatttacaa taccgtatca cacatggtga
     1021 aattaatgca gttattgcac tagagccatt cacagttgaa tttgaaaaaa ttaaagaata
     1081 tgatgcgtta actaaatttg ttattgccgg tcaaaaagat ggatggattt cacttgaagc
     1141 tgaaaaagaa aacgcaagta acaaactaga aatggcagat acgacgcgtg atgatatggc
     1201 gattctgtca tatacatctg gtacaactgg caatcctaaa gccgttacac attgtcatgg
     1261 atggggattt gcacatttac aaatggcacc aaaacattgg ttatgtatta atgaagatga
     1321 cttagtttgg gcaacagcag caccaggttg gcaaaaatgg gtatggagtc ctttcttatc
     1381 tgtattaggt tcaggtgcaa ctgcttttgt atttaatgga aggttcagtc ctgaaacata
     1441 cttagaattg cttcaaaaat atcaaattaa tgttttatgt tgtacaccga cagaatatcg
     1501 tatgatggct aaattacaga acttaaatga ttatgactta acgcatttac atagtgccgt
     1561 ttctgcaggt gagccactaa atcgagaagt agttgaacaa ttcaagaaat actttaattt
     1621 aactgtaaga gatggatatg gccagactga aagtaccttg cttataggct ttttaaaaga
     1681 tacaccacag aggattggat caatgggtaa aggcattccg ggtagttcgg tcactgtagt
     1741 agatgatgag gggaatagcg tacctgctaa tacgaaaggg aatattgcag taccacttga
     1801 tttgccagca cttttcaaag ggtattataa agagccagaa cgtacggcga aagcgcaaac
     1861 aggtaaatac tacatcactg gtgacttagc tcatgtagat gatgatggtt atttctggtt
     1921 tgaaggacgt cgagatgaca ttattattag ttcaggctat acgattgggc cttttgaagt
     1981 tgaagatgcg ttaacgaatc atccagccgt taaagaatgt gctgtagtag caaaacctca
     2041 tgatatacga ggaaatattg ttaaagcatt cgttatttta caggaccata ctgcgggtga
     2101 tgatacgtta gttaaagaac tacaacaatt tgttaaaaat gaagttgcac catataaata
     2161 tccgagagag attgaattcg ttgacgactt accaaaaaca aactcaggta aaattcgtcg
     2221 tgttgaacta cgagatgctg aaatagaaaa atggcaacaa caaaaagata gcaatcaata
     2281 aacatagcaa agcagtccaa tgcgtaaggt atgatgatac gcactggact gcttgtttat
     2341 ttatgatgtt ctttgtcgtc tagttataaa tattaaaatt agtttagaaa caagcgcaac
     2401 cagtattaag aatgagaaaa tataagatgt taaacctcga atatcccagt ttaataaaaa
     2461 cacattacta tcaccggcat taagttgaaa ctcagtatgt gttagaacga taattacaat
     2521 acctataagt gcaagattca aaataatttt caatattaaa ggtaattgta atgtttttct
     2581 tggatgcaga tattgaatat cctttttatg attgatttta cacttatatt gtatcaaaat
     2641 agagtatagg tttaaaatgc tataagccaa atgtagatta aaacaatcgt tagctatttg
     2701 ttgtcaaata att
//
LOCUS       DAKTLH010000217         2692 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.223, whole genome shotgun sequence.
ACCESSION   DAKTLH010000217 DAKTLH010000000
VERSION     DAKTLH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.223"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            290..643
                     /locus_tag="O0394_002643"
     CDS             290..643
                     /locus_tag="O0394_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCY0500688.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            711..1106
                     /locus_tag="O0394_002644"
     CDS             711..1106
                     /locus_tag="O0394_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCY0500689.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIVVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            1366..1935
                     /locus_tag="O0394_002645"
     CDS             1366..1935
                     /locus_tag="O0394_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0500690.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKAQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGVTVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISVIGILSLIIFLSVYLANKFL"
     gene            complement(2062..2262)
                     /locus_tag="O0394_002646"
     CDS             complement(2062..2262)
                     /locus_tag="O0394_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCY0500691.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
BASE COUNT          853 a          374 c          522 g          943 t
ORIGIN      
        1 gcagtagttg actgaacgaa aatgcgcttg taacaagctt ttttcaattc tagtcaacct
       61 tgctggggtg ggacgacgaa ataaattttg cgaaaatatc atttctgtcc cactccctaa
      121 tttgagctgg atatactttc atttgaaccc tttattgcta gtttatgaaa gtatcatgaa
      181 agctttatga acatcgcttg agttgccttt acagtagaaa atttaagttt tacactttgt
      241 gtgaatgata cgttttgtat tgaattaatt atagaaaggt acgttgaaga tgttttcaat
      301 tggaagtgca attcttcatt ttgtcattgg tggtatcgct gttgcattag cttcaattat
      361 tgctgataag gtaggtggta agttaggagg tattatagct actatgccgg cagtctttct
      421 tgcggctatt atcgcattag ctttagatca tcgtggtacg caattagtgg agatgtcgat
      481 gaatcttagt actggagcaa ttgtcggtat tctgtcttgt atattaactg tatttttgac
      541 atctctctac attaagcata aaggttatcg gaaaggcgca atattcacag ttgtttgttg
      601 gtttgtcatt tccctcgcaa tattcagtat tagacattta tagtttggaa aatgcgtgat
      661 aattagttgt attcagttat taagtaataa attattggag gcagaacatc atgaaattaa
      721 cattaatgaa attttttgtg gggggatttg cagtattatt aagttatatt gtatctgtaa
      781 cactaccttg gaaagaattt ggcggtatat ttgcaacgtt tccggcagta tttttagtgt
      841 ctatgtttat tacaggtatg caatatggtg ataaagtcgc tgtgcatgta agtcgtggcg
      901 cagtgtttgg tatgacaggg gtattagttt gtattttagt tacatggatg atgttacata
      961 tgacgcacat gtggttgatt agcattgttg ttggtttcct aagctggttc atcagtgcag
     1021 tatgtatttt tgaagcggta gaatttatag cacaaaaaag attagaaaag catagttgga
     1081 aagctggaaa atcgaatagt aaatagtgtg aacgtaatct cttaactagg actaactttg
     1141 caagcattga atagcatgga aaagttgcat cattaataag tgaaattcaa gttggcattg
     1201 agaaaattac aagcgcgtaa tcatacaggt ctgtcttaag ggagtcttcg aaccccgatg
     1261 ttgtcgtatg tcaaaacatt tagtcaatca taaaggtgac ttgatttaac tttatctgat
     1321 agtctgattg taatgattgt actaattgac tggaggcgta tgtaattgaa tctgagtaaa
     1381 caaattaaaa agtataggga acgagatggt tattcacaag aatatcttgc tgaaaagtta
     1441 tatgtatcta ggcagagtat ttctaattgg gaaaatgaca aaagcttacc agacatacat
     1501 aacttattaa tgatgtgtga attgttcaat gtaactttag atgatttagt aaaagggacc
     1561 attccatttg tacctgatat taaagcgcaa cgaagtctta acttatggac atatgtgatg
     1621 cttattttca tgacattagc tgcaatttta atgggacctt tagttgttta ttggaattgg
     1681 acttggggtg taacggtggc aatcattttg ggaataggtt tttatgcatc tatgaaaata
     1741 gaagatttaa aaaaagtgca taaaatggac aactacgatc gaattgttgc ttttatgaat
     1801 ggaaaagatc ctagtgaagt acaaacgact aaagctagaa atacgatgac aaatgcgctt
     1861 tctattatat cagtaattgg tatactcagc ctcataattt tccttagtgt gtatttggca
     1921 aataagtttt tataaatcat cgtggtatcg tctcatatta tttatattat ccaaaatagc
     1981 ataaaaaaat accaacaaga tttagaacct tgttggtaat caaagcgatt catttataat
     2041 gagtcgtttt atgttgtaag attaaacagt ttgtacgtta actgcttggt ctccacgttg
     2101 accttcagtg atttcgaaag taactttttg accttcttct aaagttttgt agccatcgct
     2161 agcgatacct gagaaatgta cgaatacgtc tccgccattt tcttgttcga tgaaaccaaa
     2221 acctttttct gcgttaaacc attttactgt accgttattc atatagaaaa cctccgtgtg
     2281 cttttgcact taatatttgt aacaaattca taactaaaaa agaggatatt ctaaacaaat
     2341 acactacaat ttaattcacg agcttttatt acgtaagacc aactatacgc tcatattggc
     2401 ataatgtaca gtgttttttg aaaataaatt aaaaaagatt tttaaaaacc ttagaaacgt
     2461 tgatttaaag gggtttataa aaataaaaaa attgtagtct tttatggtgt ttgctagttt
     2521 tcaaagtgac atatcgttta aacatgatga ttttataagc aatccataaa aaacaagcag
     2581 cgataaacgc tacttgttga tattaaaatc tgacttgaaa ggtcatagca atgttctata
     2641 ccgatggaat gtgcttactt gcctttttct tcacgacgtt ttaaataata ag
//
LOCUS       DAKTLH010000218         2692 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.224, whole genome shotgun sequence.
ACCESSION   DAKTLH010000218 DAKTLH010000000
VERSION     DAKTLH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.224"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..626
                     /locus_tag="O0394_002647"
     CDS             <1..626
                     /locus_tag="O0394_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCY0500692.1"
                     /translation="ASYLNIPVSIVRPYKEDLTLYQYKKGQVIYHSTDQIKFVYFLVN
                     GCILHESSNITGDNYLRLSKDENIFPMNFIFNETPAPYEICTALTDCKILTLPKDLLE
                     YLCRKHNEIFESLFKKLNETIQFQVEYIMALRANSAKERIERILQILCLSIGDDNGEF
                     YELKQIMTVQLISNLSGLNRKTTGEIIRELKIENIIYQDKRNWIIKK"
     gene            664..1662
                     /gene="argF"
                     /locus_tag="O0394_002648"
     CDS             664..1662
                     /gene="argF"
                     /locus_tag="O0394_002648"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCY0500693.1"
                     /translation="MKNLRNRNFLTLLDFTQKEMEFLLNLSEDLKRAKYAGIEQQKMK
                     GKNIALLFEKDSTRTRCAFETAAYDQGAHVTYLGPTGSQMGKKESTKDTARVLGGMYD
                     GIEYRGFSQRVVEDLAKYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKPYNEINFTY
                     VGDGRNNVANALMQGAAIMGMTFHLVCPKELNPTDELLNRCNDIADKNGGEILITDDI
                     DEGVKGSDVIYTDVWVSMGEPDEVWEKRIKLLEPYRVTKELMKKTGNPHTIFEHCLPS
                     FHDTETIIGKQIQEKYGLTEMEVTNEVFESEQSVVFQEAENRAHTIKAVMVATLGE"
     gene            1682..2611
                     /gene="arcC"
                     /locus_tag="O0394_002649"
     CDS             1682..2611
                     /gene="arcC"
                     /locus_tag="O0394_002649"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCY0500694.1"
                     /translation="MSKIVVALGGNALGQSPKEQLDLLKSTSKSLVSLIDKGYEIVIS
                     HGNGPQVGSINLGLNYAAEHKQGPPFPFPECGAMSQAYIGYQMQESLQNELHSMGIDK
                     QVVTLVTQVQVASDDSAFNNPTKPIGLFYTKEQADKFTKEKGYTFVEDSGRGYRRVVP
                     SPQPISIVELDSIETLITHGTLVIAAGGGGIPVIKENEVYTGVDAVIDKDKTSALLAA
                     HLQSDQLIILTAVDHVYINYGKENQRGLDEVSVDEMKKHISDGQFAKGSMLPKVEAAL
                     QFLEKNTKGSVLITSLAGLGDALDGKIGTLIKN"
BASE COUNT         1036 a          377 c          482 g          797 t
ORIGIN      
        1 tagcatcata cttaaatatt cctgttagta ttgttagacc ttataaagag gatttaacac
       61 tttatcaata taaaaaagga caagtcatat atcattcaac tgatcaaata aaatttgtat
      121 actttttagt aaatggttgt attttacatg aatcttctaa tattactggt gacaattatt
      181 taagattaag taaagacgaa aatatatttc caatgaactt catatttaat gaaacccctg
      241 caccatatga aatatgtaca gctttgacag attgtaaaat attaacttta ccgaaagatt
      301 tacttgagta tttatgtaga aagcataatg aaatatttga aagtctcttc aagaaactta
      361 atgagactat tcaatttcaa gtagaatata ttatggcgtt aagagctaat tcagctaaag
      421 aaagaattga aagaatacta caaattttat gcctttcaat tggggatgat aatggagaat
      481 tctatgaatt aaaacaaatt atgactgttc aattaataag taatttatct ggacttaaca
      541 gaaaaactac tggtgaaata atcagagaat taaaaataga aaatattata tatcaagata
      601 aaagaaattg gattataaaa aaataacatg aataaaatat taattgaaaa ggagatattc
      661 aaaatgaaaa atttaagaaa tagaaacttt ttaactttgt tagacttcac acaaaaagaa
      721 atggaatttt tacttaattt atctgaagat cttaaacgcg caaaatatgc aggaatagaa
      781 caacaaaaaa tgaaaggtaa aaatatcgct ctactttttg aaaaagattc aacacgcact
      841 cgatgtgcat ttgaaacagc ggcttatgat caaggtgcac atgtaacata ccttgggcca
      901 acaggttctc aaatgggtaa aaaagagtct accaaagata ctgctcgtgt tttaggtgga
      961 atgtatgatg gcatagaata tcgtggattc tcacaaagag tagttgagga tttggcaaaa
     1021 tattctggcg ttcccgtatg gaatggttta acagatgaag atcatcctac acaagtactt
     1081 gctgattttt taacagctaa agaagtattg aaaaaaccat ataatgaaat taacttcaca
     1141 tatgttggag atggacgtaa taacgttgca aatgccttaa tgcaaggagc agcaatcatg
     1201 ggcatgactt tccatcttgt atgtcctaaa gaactaaatc caactgatga attattaaat
     1261 cggtgtaatg atatagctga caaaaacggt ggtgaaatcc ttataactga tgatattgat
     1321 gaaggtgtca aaggatctga tgtaatttac acagatgttt gggtatctat gggtgaacct
     1381 gatgaagttt gggaaaaacg tatcaaatta ttagaaccat accgtgtaac taaagaatta
     1441 atgaaaaaaa caggtaatcc acatacaatt tttgaacatt gcttaccatc attccatgat
     1501 actgaaacaa taatcggtaa acaaattcaa gaaaaatatg gtctaactga aatggaagta
     1561 acaaatgaag tatttgaaag tgaacaatca gttgttttcc aagaagcaga aaatagagca
     1621 catacaataa aagcagtcat ggtagcaaca ttaggagagt aaatgaaagg aggatataaa
     1681 tatgtctaaa atcgtcgtag ctttgggtgg taacgcttta ggacaatcgc ctaaagaaca
     1741 attagattta ttaaaaagta catctaaatc tctagtaagt ttaatcgata aaggatacga
     1801 aattgtaatt agtcatggta atggtccaca agttggaagt attaacttag gtttaaatta
     1861 tgcggcggaa cacaaacaag gtcctccttt tcctttccct gaatgtggtg ctatgagtca
     1921 agcttatatt ggctatcaga tgcaagaaag tttacaaaac gaacttcatt caatgggcat
     1981 agataaacaa gttgttacgc tagtaacaca agttcaagtt gcaagcgatg attctgcttt
     2041 caataaccct actaaaccaa ttggattatt ttatacaaaa gagcaggctg ataaatttac
     2101 gaaagaaaaa ggttatactt ttgtagaaga ttctggacgc ggctatcgtc gcgttgtccc
     2161 ttccccacaa cctataagta tagttgagtt agatagtata gaaacattaa tcactcatgg
     2221 aacactagtt atcgctgccg gcggtggtgg aattccagta attaaggaaa atgaagttta
     2281 tacaggtgtt gatgcagtta ttgataaaga taaaacgagt gctttattag cagcacattt
     2341 acaatctgat caattaatca tattaactgc tgtggaccat gtttacatta actatggaaa
     2401 agaaaaccaa agaggtctcg atgaagtgtc tgtggatgaa atgaaaaaac atatctctga
     2461 tggtcaattt gctaaaggaa gtatgcttcc aaaagttgaa gctgcacttc aatttcttga
     2521 aaaaaatact aaaggcagtg ttttgattac atctctagca ggattagggg acgctttaga
     2581 cggtaaaata ggaacattaa ttaagaattg aaattataat actttagttc aattaccttt
     2641 ataaacaaaa gagcctataa tcctagggca ctgaaccctt aaaacgggaa ct
//
LOCUS       DAKTLH010000219         2680 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.225, whole genome shotgun sequence.
ACCESSION   DAKTLH010000219 DAKTLH010000000
VERSION     DAKTLH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.225"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            127..240
                     /locus_tag="O0394_002650"
     CDS             127..240
                     /locus_tag="O0394_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500695.1"
                     /translation="MYFKNSSLNYIPTTFGGEPKETLFYKVSNTLCYVNNS"
     gene            complement(244..327)
                     /locus_tag="O0394_002651"
     tRNA            complement(244..327)
                     /locus_tag="O0394_002651"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(291..293),aa:Leu,seq:caa)
     gene            complement(366..440)
                     /locus_tag="O0394_002652"
     tRNA            complement(366..440)
                     /locus_tag="O0394_002652"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(405..407),aa:Gly,seq:tcc)
     gene            complement(544..618)
                     /locus_tag="O0394_002653"
     tRNA            complement(544..618)
                     /locus_tag="O0394_002653"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(583..585),aa:Gly,seq:tcc)
     gene            complement(626..699)
                     /locus_tag="O0394_002654"
     tRNA            complement(626..699)
                     /locus_tag="O0394_002654"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(665..667),aa:Cys,seq:gca)
     gene            complement(705..776)
                     /locus_tag="O0394_002655"
     tRNA            complement(705..776)
                     /locus_tag="O0394_002655"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(742..744),aa:Gln,seq:ttg)
     gene            complement(788..860)
                     /locus_tag="O0394_002656"
     tRNA            complement(788..860)
                     /locus_tag="O0394_002656"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(825..827),aa:His,seq:gtg)
     gene            complement(863..936)
                     /locus_tag="O0394_002657"
     tRNA            complement(863..936)
                     /locus_tag="O0394_002657"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Trp,seq:cca)
     gene            complement(952..1032)
                     /locus_tag="O0394_002658"
     tRNA            complement(952..1032)
                     /locus_tag="O0394_002658"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(996..998),aa:Tyr,seq:gta)
     gene            complement(1038..1113)
                     /locus_tag="O0394_002659"
     tRNA            complement(1038..1113)
                     /locus_tag="O0394_002659"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1078..1080),aa:Thr,seq:tgt)
     gene            complement(1121..1193)
                     /locus_tag="O0394_002660"
     tRNA            complement(1121..1193)
                     /locus_tag="O0394_002660"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1158..1160),aa:Phe,seq:gaa)
     gene            complement(1212..1287)
                     /locus_tag="O0394_002661"
     tRNA            complement(1212..1287)
                     /locus_tag="O0394_002661"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1252..1254),aa:Asp,seq:gtc)
     gene            complement(1297..1370)
                     /locus_tag="O0394_002662"
     tRNA            complement(1297..1370)
                     /locus_tag="O0394_002662"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1334..1336),aa:Met,seq:cat)
     gene            complement(1385..1477)
                     /locus_tag="O0394_002663"
     tRNA            complement(1385..1477)
                     /locus_tag="O0394_002663"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1439..1441),aa:Ser,seq:tga)
     gene            complement(1516..1591)
                     /locus_tag="O0394_002664"
     tRNA            complement(1516..1591)
                     /locus_tag="O0394_002664"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1556..1558),aa:Asp,seq:gtc)
     gene            complement(1602..1691)
                     /locus_tag="O0394_002665"
     tRNA            complement(1602..1691)
                     /locus_tag="O0394_002665"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1653..1655),aa:Ser,seq:tga)
     gene            complement(1701..1774)
                     /locus_tag="O0394_002666"
     tRNA            complement(1701..1774)
                     /locus_tag="O0394_002666"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1738..1740),aa:Ile,seq:cat)
     gene            complement(1803..1876)
                     /locus_tag="O0394_002667"
     tRNA            complement(1803..1876)
                     /locus_tag="O0394_002667"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1840..1842),aa:Met,seq:cat)
     gene            complement(1898..1973)
                     /locus_tag="O0394_002668"
     tRNA            complement(1898..1973)
                     /locus_tag="O0394_002668"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Ala,seq:tgc)
     gene            complement(1991..2064)
                     /locus_tag="O0394_002669"
     tRNA            complement(1991..2064)
                     /locus_tag="O0394_002669"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2028..2030),aa:Pro,seq:tgg)
     gene            complement(2074..2147)
                     /locus_tag="O0394_002670"
     tRNA            complement(2074..2147)
                     /locus_tag="O0394_002670"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2111..2113),aa:Arg,seq:acg)
     gene            complement(2166..2254)
                     /locus_tag="O0394_002671"
     tRNA            complement(2166..2254)
                     /locus_tag="O0394_002671"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2218..2220),aa:Leu,seq:taa)
     gene            complement(2265..2339)
                     /locus_tag="O0394_002672"
     tRNA            complement(2265..2339)
                     /locus_tag="O0394_002672"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2305..2307),aa:Gly,seq:gcc)
     gene            complement(2343..2424)
                     /locus_tag="O0394_002673"
     tRNA            complement(2343..2424)
                     /locus_tag="O0394_002673"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2388..2390),aa:Leu,seq:tag)
     gene            complement(2428..2503)
                     /locus_tag="O0394_002674"
     tRNA            complement(2428..2503)
                     /locus_tag="O0394_002674"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2468..2470),aa:Lys,seq:ttt)
     gene            complement(2509..2584)
                     /locus_tag="O0394_002675"
     tRNA            complement(2509..2584)
                     /locus_tag="O0394_002675"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2549..2551),aa:Thr,seq:tgt)
     gene            complement(2601..2676)
                     /locus_tag="O0394_002676"
     tRNA            complement(2601..2676)
                     /locus_tag="O0394_002676"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2641..2643),aa:Val,seq:tac)
BASE COUNT          764 a          678 c          621 g          617 t
ORIGIN      
        1 tggttacaga aatttcaaca actttaaagc acgtataatg atgattttca gcttgtacaa
       61 aggagaaaaa agaagacaac caagcccaat aatggactgg ccgcctaata ataaaaactc
      121 taaaagttgt attttaaaaa tagttcttta aattatatac ccaccacatt tggtggagaa
      181 ccaaaagaaa ccttgtttta caaggtttct aatacgttat gttatgtaaa taacagttaa
      241 ttataccggt ggtcggggtc gaaccgacac tccacaagtg gaacgggatt ttgagtcccg
      301 cgcgtctgcc aattccgcca caccggctta atggtaaaca aaaaacttcc ctttggaagc
      361 aattatggag cggaagatag gatttacacc tatacctcgt tccgggaagg aacgtgttct
      421 aaaagttgaa ctactcccgc aaatattaaa ttatggagcg gaagatagga tttacaccta
      481 tacctcattc caggaaggaa tgtattctaa gagttgaaat actcccgcat tattattaaa
      541 ttatggagcg gaagatagga tttgcaccta tacctcgttc cgggaaggaa cgtgttctaa
      601 aagttgaact actcccgcat aaacctggag gcggcaaccg gatttgaacc ggtgataaag
      661 gttttgcaga cctctgcctt accacttggc tatgccgcca ataactgggc tagctggatt
      721 cgaaccaacg agtgacggag tcaaagtccg ttgccttacc gcttggctat agcccattaa
      781 taataagggc ggctgaaggg gatcgaaccc tcgaatgtcg gaaccacaat ccgatgtgtt
      841 aaccacttca ccacagccgc catggcaggg gcagtaggaa tcgaacccac accaaaggtt
      901 ttggagacct ctattctacc gttgaactat gcccctatta aaaataataa atggaggggg
      961 gcagattcga actgccgaac ccgaaggagc ggatttacag tccgccgcgt ttagccactt
     1021 cgctacccct ccataaatgg tgccggccag aggacttgaa cccccaacct actgattaca
     1081 agtcagttgc tctaccaatt gagctaggcc ggctaagaaa tggttcagga cagagtcgaa
     1141 ctgccgacac atggagcttc aatccattgc tctaccaact gagctactga accataataa
     1201 aaatgtaatg atggcggtct cgacgggaat cgaacccgcg atctcctgcg tgacaggcag
     1261 gcgtgttaac cgctacacta cgagacctat aaaatattgc gggaggcgga tttgaaccac
     1321 cgaccttcgg gttatgagcc cgacgagcta ccgaactgct ccatcccgcg ataataaaaa
     1381 ataatggcgg aggaagaggg attcgaaccc ccgcggcccg ttaaggccct gtcggttttc
     1441 aagaccgatc ccttcagccg gacttgggta ttcctccatt attataggta aatcgctatt
     1501 aattataaaa ttaaatggcg gtctcgacgg gaatcgaacc cgcgatctcc tgcgtgacag
     1561 gcaggcgtgt taaccgctac actacgagac cattagtaaa acggaggaag agggattcga
     1621 acccccgcga gccgttaagc ccctgtcggt tttcaagacc gatcccttca gccggacttg
     1681 ggtattcctc caaaattata tggaccttgc aggactcgaa cctgcgaccg aacggttatg
     1741 agccgttagc tctaaccaac tgagctaaag gtcctaaata taattttaca actaataaat
     1801 agtggcggtg gaggggatcg aacccccgac ctcacgggta tgaaccgtac gctctagcca
     1861 gctgagctac accgccttat atagtttgta aataatatgg tggagactag cgggatcgaa
     1921 ccgctgacct cctgcgtgca aagcaggcgc tctcccagct gagctaagcc cccataataa
     1981 ttacagtata tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc
     2041 tctaccaagc tgagctactt cccgtataat taacgcgccc gataggagtc gaacccataa
     2101 cctcttgatc cgtagtcaaa cgctctatcc aattgagcta cgggcgcata tgtttttatt
     2161 gaaaatggtg ccgaggaccg gaatcgaacc ggtacggtga tcactcaccg caggatttta
     2221 agtcctgtgc gtctgccagt tccgccaccc cggcactata aaaatggagc agaagacggg
     2281 attcgaaccc gcgaccccaa ccttggcaag gttgtattct accgctgaac tacttctgca
     2341 tatgcgggtg aagggagtcg aacccccacg ccgtaaggcg ctagatccta agtctagtgc
     2401 gtctgccaat tccgccacac ccgcaaatgg tgagccatag aggattcgaa cctctgaccc
     2461 tctgattaaa agtcagatgc tctaccaact gagctaatgg ctcttccatg gtgccggcca
     2521 gaggacttga acccccaacc tactgattac aagtcagttg ctctaccaat tgagctaggc
     2581 cggcaatatg taagaataaa tggtggagaa tgacgggttc gaaccgccga ccctctgctt
     2641 gtaaggcaga tgctctccca gctgagctaa ttctccgatt
//
LOCUS       DAKTLH010000220         2669 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.226, whole genome shotgun sequence.
ACCESSION   DAKTLH010000220 DAKTLH010000000
VERSION     DAKTLH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.226"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..430)
                     /locus_tag="O0394_002677"
     CDS             complement(<1..430)
                     /locus_tag="O0394_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCY0500696.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEH"
     gene            497..967
                     /locus_tag="O0394_002678"
     CDS             497..967
                     /locus_tag="O0394_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HCY0500697.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            1114..1983
                     /locus_tag="O0394_002679"
     CDS             1114..1983
                     /locus_tag="O0394_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0500698.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKEVMSIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            2004..2570
                     /locus_tag="O0394_002680"
     CDS             2004..2570
                     /locus_tag="O0394_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCY0500699.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
BASE COUNT         1005 a          367 c          472 g          825 t
ORIGIN      
        1 gatgctcaaa actacggtta cgtttagcaa ttctagattt taacacatca aggtctgcat
       61 ctaaaaagat aatcatattc ggcataatca tatcttcagt taaaatatca taaattttac
      121 tgaatttctg aaattcaaca gaactcaaag tatttttagc aaatatctta tttttatgta
      181 tatgataatc actaactaca ccttgattta gttgtgttac atcttgaaat tgcttatatc
      241 tattgcataa aaagaacatt tcagtttgaa aactccattt agagatatct tcataaaagt
      301 ctgataaaaa tggattttct gtgatgattt ctttttcttc ataaaaatct aaagtttgac
      361 ttaatttgtg tgcaagtgaa gatttaccta cgccaatagg accttcaatt gctataaaag
      421 gtttgttcat attcacatct ccaaatgtta aaatagacat tgagtattgt accataagtg
      481 aggtatctgg acataaatga caaatgatat atattttatg acattagcga ttgaagaagc
      541 taaaaaagca gctcaactag gcgaagtacc tataggtgct atcatcacta aagatgatga
      601 agttatcgct agagcacata atttaagaga aacactacaa caaccaacgg cgcatgctga
      661 acatattgca attgaacgtg cagccaaagt gttaggtagt tggcgtttag aaggttgcac
      721 attatatgta accttagaac catgtgtcat gtgcgcagga acaattgtaa tgagtcgcat
      781 tccaagagtc gtctatggcg cagatgatcc taaaggtggt tgtagtggca gtttaatgaa
      841 tttattgcaa caatctaatt ttaatcatcg tgcaattgtt gataaaggtg tacttaaaga
      901 agcatgtagc acattattaa caacattttt taaaaactta agagccaata agaaatccac
      961 caattagacg atagtacata ctaattaaaa tttgttagaa ttacattata tgataaatta
     1021 atgacaatat aaatgttaat agaaaagtat gtgcgctgag tatatattct tatttaagaa
     1081 caagatttta aatttacgaa acgaggtaac ataatgataa aactaatagc cactgatatg
     1141 gatggcacgc tacttaatgc agcacatgaa atttctcaac ctaatattga tgcgattaaa
     1201 tacgctcaag aacaagggat aacggttgtt atcgcgacag gtcgagcatt ttatgaagca
     1261 caagcaccag ttgctgacac agatttaaca gtaccatata tttgtttgaa tggtgctgaa
     1321 gtacgtgatg aaactttcaa tgtaatgagc acttcacacc ttaataaatc gttagtacac
     1381 aaaattacaa atgttttaaa agatgcaggt atttattatc aagtatacac gagtcgtgcg
     1441 atttatactg aagatccaca aagagattta gacatttaca tagatattgc tgagcgtgca
     1501 ggtcaacatg caaacgttga gcgtattaaa aatggtattc aaagacgcat agataatggt
     1561 acgttgaaag ttgttgataa ttatgatgct attgaaaaca tacctggtga attaattatg
     1621 aaaatattag catttgatgg aaatttagaa aaaattgaca aagctagtaa aattttagct
     1681 gaatctccga atttagctat atcatcatct tcgagaggaa atatagaaat aacgcattca
     1741 gatgcacaaa aaggtattgc gctagaaaca attgccgaaa gattagggat tgaaatgaaa
     1801 gaagtcatgt caataggtga caatttaaat gacttatcaa tgttagagaa agttggctat
     1861 ccagttgcga tggaaaatgg tgcagaagaa gttaaaaaaa tagcgaaata tgtcacagat
     1921 acgaatgaaa atagtggtgt tggaaaagct attatgaaat tattacgtga acaacaagtt
     1981 taataaaaaa agaggggtca aatatgaaag gattaattat tattggcagt gcacaagtga
     2041 attcacatac aagtgcacta gcaagatact taactgagca ttttaaaaca catgatattg
     2101 aagcggaaat attcgattta gcagaaaaac cgttaaatca attagatttt tcaggaacaa
     2161 caccgtctat tgatgaaatc aaacaaaata tgaaagattt aaaagagaaa gcaatggcgg
     2221 cggacttttt aatattagga acgccaaact atcatggttc atattctgga atattgaaaa
     2281 atgcattaga tcatctaaat atggattatt ttaaaatgaa acctgtaggc ttaataggaa
     2341 atagtggtgg tattgttagt tcagagccat tgtcacattt aagagtaatc gtcagaagtt
     2401 tactaggcat tgctgtacca actcaaatag caacacatga ttctgatttt gctaaaaatg
     2461 aagatggttc atattactta aatgatagtg aattccaatt acgagcaaga ttatttgtcg
     2521 atcaaattgt atcttttgtg aataatagtc catatgaaca tttaaaataa tattaaaaaa
     2581 tatgtaaata tacattaaaa ataggtatgt agcttaaggt atttcattga gaatgctttt
     2641 aagtcacatg cctatttttt gttctaatg
//
LOCUS       DAKTLH010000221         2654 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.227, whole genome shotgun sequence.
ACCESSION   DAKTLH010000221 DAKTLH010000000
VERSION     DAKTLH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.227"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..541)
                     /locus_tag="O0394_002681"
     CDS             complement(<1..541)
                     /locus_tag="O0394_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500700.1"
                     /translation="MKFTEVEVIEHLVKAYKEAGKPTYPHENLYRGRNHSISGIGEDL
                     LGAYLISRLEGVQIFIDQPLSMIDKSLSTRYPDLLICEDNEIKNILEVKMDLGYQRKD
                     FIDYCRKKEEWISNIVGKQCVLSRKREDKIPMNIADDIKFHVVIYSENNGPKRFDEEI
                     MPIVNETCPHIEVYVLTSGQ"
     gene            complement(670..1041)
                     /locus_tag="O0394_002682"
     CDS             complement(670..1041)
                     /locus_tag="O0394_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0500701.1"
                     /translation="MKTNILNLDNELENTEYVEGKPYVQYGIKYERNQALRNEAIKIH
                     GTTCKVCGFDFKAKYGDLGEGFIEIHHLKPMFSIKREIKVNPQKDLVPLCSNCHKMIH
                     RNTKKPLTIKELTKIVNYNSK"
     gene            complement(1038..1175)
                     /locus_tag="O0394_002683"
     CDS             complement(1038..1175)
                     /locus_tag="O0394_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001791883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500702.1"
                     /translation="MELKLLNKLDDDQLDLEFLQDNGLKGNVQGPMTLKEPLKSYIQK
                     S"
     gene            1231..2549
                     /gene="cstB"
                     /locus_tag="O0394_002684"
                     /pseudo
     CDS             1231..2549
                     /gene="cstB"
                     /locus_tag="O0394_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002488497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
BASE COUNT          901 a          442 c          368 g          943 t
ORIGIN      
        1 gttgaccgct tgttaggaca tatacttcaa tatgtggaca tgtttcatta acgataggca
       61 tgatttcttc atcaaaccgc ttcggtccat tgttttcact gtaaatcaca acatgaaatt
      121 taatatcatc agctatattc ataggaattt tgtcttctct ctttctagac aatacacact
      181 gttttcctac gatatttgaa atccattctt ctttctttcg gcaataatct ataaaatctt
      241 ttctttgata tcctaagtcc attttaactt ctagtatatt tttaatttca ttatcttcac
      301 aaattaataa atccggatat cttgtactta aagatttatc aatcatagat aaaggctgat
      361 caataaatat ttggacacct tccaatctac taatcaaata agcacccagc aagtcttctc
      421 caatacctga aatactatga ttacgtcctc gatataaatt ttcatgagga taagtaggct
      481 ttcctgcttc tttatatgcc tttacaagat gttcgataac ttccacttca gtgaatttca
      541 tactcaatca ccctatctat agatactcta tatttcaaca aacaaagtat atcacacatt
      601 tgaaagcata aatacaaaaa acctcctaat aaattaagag ggttgaatga tagtttataa
      661 aatattaaat tatttgctat tataattaac tattttggtt aattctttaa tcgttaaagg
      721 ttttttagta tttctatgaa tcattttatg gcaattagaa catagtggga ctaaatcttt
      781 ttgtggattt acttttattt ctctttttat tgaaaacatt ggttttaaat gatgaatttc
      841 aataaaaccc tcacctaaat cgccatactt agctttaaaa tcaaatccac atactttaca
      901 tgtagttcca tgaattttaa tagcttcatt tcttaatgcc tgatttcttt catatttaat
      961 accgtactgt acatatggtt taccttcgac atattcagtg ttttctaact cattatctaa
     1021 atttaaaata ttcgttttca agacttttgt atataacttt ttaaaggttc ctttaaagtc
     1081 ataggacctt gaacattacc ttttaaccca ttatcttgta aaaattctaa atctaattgg
     1141 tcatcatcaa gtttgtttaa taactttaat tccatgtaat taccatagtt aatataatta
     1201 tcacccttga taacaaattg actatcatca atttatgata aacatttatc tcaagcatct
     1261 tatttaatcg gttgtcaaaa aactggagaa gccatgatca ttgatcctat ttgtgactta
     1321 tcttcatata ttcgagttgc cgatgaagag agtttaacca ttactcatgc agctgaaaca
     1381 catatacatg caaattttgt ttcaggaatt agagatgttg ctataaagtt aaatgctagt
     1441 atttatgtat cgggcgaaag tgatgacatg ttaggttata aaaatatgcc taaccacact
     1501 cattttgttc aacataatga tgatatttat gtaggaaata taaaattaaa agtgcttcat
     1561 acacctggtc acacgccaga aagtataagt tttttactta ctgacgaagg tgctggagca
     1621 caagttccaa tgggactatt cagtggtgat tttatttttg taggagatat cggtagacct
     1681 gatttactag aaaaagctgt taaagtagaa ggattatctg aaataggcgc aaaacaaatg
     1741 tttaaatcta ttgaaagtat taaagacttg ccagactaca ttcaaatttg gcctggtcat
     1801 ggcgctggta gtccttgtgg taaatcttta ggtgctattc caacatctac gcttggctat
     1861 gaaaaacaaa cgaactgggc tttttctgaa aataatgaag ctacctttat cgataaactc
     1921 atttctgacc aacctgcacc accacatcat tttgcacaaa tgaaaaaaat taatcaattc
     1981 ggtatgaatt tatatcaacc ttatacggtt tatccagcta caagtacaaa cagattaact
     2041 tttgatctcc gcagtaagga ggcttatcat ggtggacata ttgaaggtac aatcaatatt
     2101 ccatatgata aaaatttcat caatcaaatt ggctggtatc taaactataa tcaagaaatt
     2161 aacttgattg gagactatca ccttgtttca aaagcaacac ataacatact ttacaactta
     2221 ttggatatga taatgtgtct ggatatcagt taccacaagc tcaaattcaa acacaatctg
     2281 ttcatagtaa agacataact ggtaatgaag cacatgtctt agatgtacgt aatgataatg
     2341 aatggaataa tggccactta tcccaagcgg ttcatgtgcc acacggcaaa cttttagaaa
     2401 cagatttacc tttcaataga aacgatgtta tttatgtaga ctgtcagtct ggcattagaa
     2461 gttctatagc tattggtatt ttagaacaca aaggttatca caacattatt aatgtaaacg
     2521 aaggttacaa agatatatac ctttcttaat aattatattt atactaatga agaaggacta
     2581 taagtagtta gatatagctt ttagatatag cttatagttc ctttttatat tattggataa
     2641 atatctattt aaaa
//
LOCUS       DAKTLH010000222         2648 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.228, whole genome shotgun sequence.
ACCESSION   DAKTLH010000222 DAKTLH010000000
VERSION     DAKTLH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.228"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            112..2261
                     /locus_tag="O0394_002685"
                     /pseudo
     CDS             112..2261
                     /locus_tag="O0394_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            2407..>2648
                     /gene="sarX"
                     /locus_tag="O0394_002686"
     CDS             2407..>2648
                     /gene="sarX"
                     /locus_tag="O0394_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HCY0500703.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLLVSIRRLIEKE"
BASE COUNT         1057 a          301 c          404 g          886 t
ORIGIN      
        1 atttattttt gatttggtat tcgtattgtg ttaaatttac gtttaattaa ataagttaaa
       61 tattataggg aatgataaaa gtggtactta aatatagaaa gaggtaaagt tatggcaaaa
      121 tcatgcttgc atatacttac taataatgaa tatgcgacaa cgcgttgcca agatggcata
      181 gtcttatttt ggccaattga cggggaaatc gaactacaaa aatttcgtaa aagtaaaata
      241 attgaagatg atatatatat tattaatcat ctggatgtat ttagtattaa gaataataaa
      301 aaaacgatca tgttgtattt gagtagcgat tggtttgcgg aattaggctt tactttcttt
      361 aattaccact atacagcaaa gttgattaaa tcatcctata atttgaaatg tctactatta
      421 aaattgacat atcgatacct tgataatcag cctcttaatg acgctgatat tagaaaatta
      481 caggatatta ttaaaatcat tgcaaaagaa gcaagtatgg ataaaaagat tgcacaaaat
      541 caatatcgat atgcgtatta tggtgatttg cgtgatgagc tcgaatatat ttatcaaaat
      601 gtaaatcaac gattgacatt aaaaagtgtc gctgataaat tatttgtctc aaagtcaaat
      661 ttgtcatcac aattccactt acttatgggc atgggtttta aaaaatatat tgatactttg
      721 aaaattggta aatcgattga aattctactt actactgata gtactattag caacataagt
      781 gaacatttag gttttagtag tagctccact tactctaaaa tgtttaaaag ttatatggat
      841 ataacaccga atgaatatcg taatttatca aaatataata aatgtttaat gctaaagcca
      901 gaaccactag taggcaaaat ggtgcaagaa gtaaaagaaa tcatattgaa ttatattgaa
      961 cattataaaa accacctaac tgatgttata catattgatg aagacaaatt tgaaacacct
     1021 aaattgtttc aaacggttat tcaaataaat acttatacag aaatgaaatt agttttctta
     1081 gaaggaatct ttaaaacctt attgaataag aacagtcaag ttgtcttttt catcatgcca
     1141 tcgattctaa aaagtaaaaa taccatgtcc gaagaagaaa aattcacaat cattaaaaca
     1201 ataattgaaa gtgatctaaa gatagcattt aatataaata atattgaaac aacttatttt
     1261 gttgaagaag cttttatgag tgttttcaga caaatatctc caaacgaatt aagtaatcat
     1321 aataattacg aagtgcattt tgtttttgat ttatcattga tggaaattag aacaatttat
     1381 cgaatgatat taaaattaca taacatcatg ttgaatgtga aattaggatt gaacattacc
     1441 tgtttatttg aaaaaccttc agtttttaaa tcactagtat cacaaataaa gcgacttaaa
     1501 ttcgattcgt taataataga taatgcaaat ttaagtagcc cttatttgat gggggaaagt
     1561 gatgagttac tattgaaaaa tattttgcat tttaaaaatt taaaacaagt aattaatgaa
     1621 ttggatattg aacaagaaaa gctgattttt ctaaatgttg aaaatcataa actgcttaat
     1681 aataaagaac gagatctaag taatagtgct ccattaattt ataagacatt aagtgcgctg
     1741 tatcacaact ttgatggctt tggattaaac atttttgaca atcatcaagc atttaatgcg
     1801 atgcatctat atgacaaaaa tggatttaaa acaacactag gtcttatatt agaaaagttt
     1861 atcgaatatg tctcgaaacc aaaatacgaa aacagttatt attctatttt tgatatagag
     1921 aattattatt gtcttgttat ttatgattgg cgagtgatag agagcgagac aattatgagt
     1981 aattttgagg atagtcaagt ttatataaat tttaaaaaca atgttttaaa cgataaatat
     2041 ctaattgtaa tagaaacatt ggacaaaata gtggcaacat taatcatttg atttctaaag
     2101 aattaagaga taaatatgaa tggaacccta gtttactatc taaaattgac aactacctta
     2161 aaccagcaat agagattaaa gagcataatt ttagtgataa ttccttgaat attaacgtta
     2221 cttttaatgc gttatacata attaaaatag gaaaaaaata acaccttgat atgtattgca
     2281 aaatttaatt tgcatttgtt ggtaattttg acaacgtatt aaaaacaaat gagaaagaca
     2341 atggctgaaa aatttaagat aataatgaca gaagcattgt ctttatatat ttgggggtgc
     2401 aacattttga atactgagaa attagaaaca ttgcttggct tctataaaca atataaagca
     2461 ttatctgaat atattgataa aaaatataag ttgtcgctaa atgatttagc agtcttagat
     2521 ttaacgatga agcattgcaa agatgaaaaa gtacttatgc aatcattttt aaaaactgca
     2581 atggatgagc tagatttaag taggacaaaa ttattagttt ctataagaag actaattgaa
     2641 aaagaaag
//
LOCUS       DAKTLH010000223         2627 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.229, whole genome shotgun sequence.
ACCESSION   DAKTLH010000223 DAKTLH010000000
VERSION     DAKTLH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.229"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            246..1043
                     /locus_tag="O0394_002687"
     CDS             246..1043
                     /locus_tag="O0394_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCY0500704.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
     gene            complement(1631..>2627)
                     /locus_tag="O0394_002688"
     CDS             complement(1631..>2627)
                     /locus_tag="O0394_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HCY0500705.1"
                     /translation="IIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKTAILM
                     AAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYGIPSS
                     SSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFKVIFK
                     NANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVEPQLW
                     VKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLHFPLS
                     TTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLFF"
BASE COUNT          932 a          465 c          396 g          834 t
ORIGIN      
        1 atataatttt ttataattag ttaaaatcac taaaacgctt ttatacacta tcaaatcagc
       61 atttataaaa atatgaaccg atatcctaaa atgttaataa tattacaaga taataacaaa
      121 ccacacaaag ctacttattt ttgataatat ggaaatcgta atataaaacg aaaacttaat
      181 ttactatata aattgtctta ataattttta aaagtagtaa aacataattt taaggaggag
      241 tccctttgaa aaaattagca tttgcaataa cagcaacatc tggtgcagct gcatttttaa
      301 cgcatcatga tgcacaagct tctacacaac atacagtaca atctggtgaa tcattatgga
      361 gtattgctca aaaatacaac acttcagtag agagtattaa acaaaataac caattagata
      421 acaacttggt attccctggt caagttatct cagtaggtgg aagtgatgca caaaatacgt
      481 caaacacttc tccacaagct ggttcagcat catctcatac tgtacaagct ggtgaatcat
      541 taaatatcat tgctagcaga tatggtgttt cagttgatca attaatggca gccaataact
      601 tacgtggtta tttaattatg cctaaccaaa cattacaaat tcctaatggt ggatcaggtg
      661 gtacaacacc aacagctaca acaggtagca atggcaatgc atcatctttt aatcaccaaa
      721 atttatacac tgctggtcaa tgtacatggt acgtatttga ccgtcgtgct caagctggta
      781 gtccaattag cacatattgg tcagacgcta agtattgggc tggtaacgca gctaatgatg
      841 gttaccaagt aaacaacaca ccatcagttg gttcaattat gcaaagcaca cctggtccat
      901 atggtcatgt tgcttatgtt gaacgtgtca atggtgatgg tagtatcttg atttctgaaa
      961 tgaattacac atatggtcca tacaatatga actaccgtac aattccagct tcagaagttt
     1021 ctagctatgc attcatccat taattaaata aattgtactg atatatacta gcaattcaca
     1081 tcatgtgaga ttgctagttt tttatttttg aaaaaaattt tcattttggt acaaaaaatt
     1141 atctcaccct tccctatcat acatatttat attttgtatg aatggtagtt aggtaaaaat
     1201 taacaaccta cctatttgat taaattaaat gcataatata ttaaaaatca aaataatgca
     1261 actaagttga ctatataatg atttaatttg ttataccagt attttacgct ttttcgtcta
     1321 catatacaaa tttatattaa ataaagccca atacaattta ggttaattaa acaagttgat
     1381 aactatttaa ttattccttc attgaagaat ataaactatt aaatcattat tttgctctta
     1441 catatatttt aatgacctaa ctgattatgt tccatggaat acatttataa tatagcctcc
     1501 taattaaaat gctttgtctt ggtcattcta cgtaaattct ataaaatatg ttatctactt
     1561 acataaaaaa ctgaacttca ataccaccat atgtttgtga tactgaagtt cagtttagtt
     1621 ttattttcaa ttagaaaaat aagttaagta tatagaatag taaacctgct aacaatgctg
     1681 aaataggtaa tgtaatcacc catgtaatga tcattcgttg cgcagtgctc cattttacac
     1741 ctttagctcg gttagaagca ccaacaccta agattgatga tgacacaacg tgagttgttg
     1801 ataatgggaa atgtagcgat gatgcaacaa aaattgttaa tgcagatgat aaatcggccg
     1861 cagcaccatt tgctggacgt attttcataa tattaccacc tacagttttg ataattttcc
     1921 agccaccaat tgcagtacca agccccattg ctgtcgcaca ggcaaatttt acccataact
     1981 gtggttcaac actgccatca ttctgtacat tagcgacaat caatgccaac gtaataatac
     2041 ccattgattt ttgcgcatca ttcgtaccgt gagagaatga ttgtaacgct gctgtgaaaa
     2101 tttggaaaaa tctaaagtta cgattcgctc ttgttaaatt tgcattttta aagataactt
     2161 taaaaattga atacatcaag aaaccaacac aaaatgcgat aatcggtgaa acgattaata
     2221 caataataat ttttgtgaaa ccttggtaat gtaacactcc aaatgagcct tcagatgcga
     2281 ttgctgcacc cgcaattgaa cctataagtg catgtgaaga cgaacttgga attccgtaaa
     2341 accaagtagc taaattccaa ataatagccg caagtattgc agctaacaca acaactaatc
     2401 cattttccaa tttaaatgga tcgacaatgt ctttagtaat ggtgcctgca acgcccgtaa
     2461 atgttaaagc acctataaag ttcatcactg ctgccattaa aattgccgtt ttaggcgtta
     2521 acgctctagt agatacagca gtagctactg cattggctgt atcatggaat ccattgataa
     2581 agtcaaatat cagcgagaaa ataactacag ctatagtgac gatgatt
//
LOCUS       DAKTLH010000224         2592 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.230, whole genome shotgun sequence.
ACCESSION   DAKTLH010000224 DAKTLH010000000
VERSION     DAKTLH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.230"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(133..831)
                     /locus_tag="O0394_002689"
     CDS             complement(133..831)
                     /locus_tag="O0394_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HCY0500706.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQQETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(848..1837)
                     /gene="floA"
                     /locus_tag="O0394_002690"
     CDS             complement(848..1837)
                     /gene="floA"
                     /locus_tag="O0394_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HCY0500707.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            complement(1855..2538)
                     /locus_tag="O0394_002691"
     CDS             complement(1855..2538)
                     /locus_tag="O0394_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCY0500708.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
BASE COUNT          781 a          502 c          377 g          932 t
ORIGIN      
        1 tttagactaa tcaatttttt gctgtaattt aaataactaa tatttctcga aaaattctct
       61 ttgtttttta atttttctta acgtaaaaaa acaagcaact accattacga tagtgcttgt
      121 tttcaaaaaa ttttataatt gttttggttt agctaaaatt tctgaccata tcataccatt
      181 gattacttca tctttatcaa attggaacgc tgatttagtc aaatcttttt cgacattttg
      241 agaattaagc agctgcttta atttcaaacg ttttgtacgt tcagataagt atttatcctc
      301 aataatatct ctagcacgtt tttccatttt agcaatttgc ttttcttttt ctctatcaat
      361 gtcagtacga atcgttttaa tatcatctct aagtgatttt tctaattgac gtctaatttc
      421 gtcagaatta tcttgtctag aaggtctatg tttctgctcc accggttctg ctttaggcac
      481 tgtgattggt ttctctgtca tcggttttgt ttcttgttgt tttttaggtg ccataggttc
      541 tacaacaggt ttcgatttag gctcttcctt tggaagttca tcgaataaag gtggtaacgt
      601 atcatcatat tttcgtttcg atgatttctt ttcttcttca tttaattctt cacttatttc
      661 tttaaacgtt cgctcaattt cttcaaaaaa gccacctttt tttggttcat tatcggtaga
      721 tgttttttga ggtggctttt gattttgtct atctttatga ctattttcgc gcatagtagt
      781 aatgatagaa atgatcactg atatgacaaa aattagaata ccgacactca tttaatcacc
      841 tctcgactta atgttcaggt gactcatcat cactttgatc agttcgttta ttaattgcat
      901 ttctcatgcc tgtatcagct tcgatatttt tcaaattata ataatcttta acactgatat
      961 tacctgaacg taatgcttca gccatagcta atggtacttc agattcggct tcaactactt
     1021 tagcatgcat ttcttgtaca cgcgctttca tttcttgctc agttgctaca gccatagctc
     1081 tacgttcttc agcttttgct tgtgcaatat ttttgtctgc taatgcttgt tcagtttgta
     1141 agtctgcacc aatattttta ctaatatcaa cgtcagcaat atcaattgat aaaatttcaa
     1201 atgcagtacc tgaatctaaa cctttgctta aaactgtttt agaaatatta tctgggtttt
     1261 caagtacttc tgtatgatgc ttactagaac caattgttga aacgatacct tcaccaacac
     1321 gtgcgatgat tgtttcttca ccagcaccac caacaagtcg agcaatatta gctctaactg
     1381 tgatacgagc tttggctttc acttcaatac cgttcattgc tacacctgcg ataaatggtg
     1441 tttcaatgac tttaggatta acagacattt gaaccgcttc taatacgtca cgtcctgcaa
     1501 ggtcaattgc agcagcacgt tcgaaaggaa gatcaatgtc agcacgttgt gcagcaatat
     1561 tagcgtcaac aactctgtca acatttcctc ctgctagata atgcgattct aattggtttg
     1621 ttgttaatgc tagtcctgct ttgtgcgctt taattaatgg cgctataact tttcttggag
     1681 atacacgacg taaacgcata ccaaccaatg tacctatacc aacatgaacg ccagctgcta
     1741 acgctgaaat ccataaacca atgggtacaa atgagaataa aataagtaat gcaactacta
     1801 taataactgc tattacgata aaacttaaac taaacatggt atcgctcctt ttaattaatc
     1861 tacttccctc acaactactc ttgttccttc aacttcaagg atttttaccg ttttattgcg
     1921 caaaataaag ttgccatctg aaacagcatc aatacgttca ttttcacaaa aaataatccc
     1981 tgcaggtcga agatctgtaa ctgtttgagc agtctttcct acgaggtgcg agcggttatc
     2041 atgagaattg taacctgact cagaattagt tgaatctttt aagataactt tatccaaaaa
     2101 cggaatcttt ctgttgaaaa tcttcactaa tatcacccat tctacaatcg ttaaaatcaa
     2161 ggcaacgata acattcgcaa gcataaatag caaattatca ccgagcgttg ttatgcttat
     2221 agttatcagt atcatgccaa taataccaat tactgcacca actacaaata attcaattac
     2281 aactaatata acgccaattg agaatattaa gatagaatgc atattgacat ttccttggat
     2341 tagaaatccc aaaaataaaa taagtaatga taatgtggcg ataataccag ctgcattgat
     2401 ttttttagag taaagttgat atacaaatcc taagaatgtc aaacaggtta atattaacgt
     2461 aaaaataggt tgaacaatta tattgctaac ttgttcaacc caagtatctc cagccgttga
     2521 ttccaagata gttgtcattt gtaaaaaatt attataactc acaatttcac ctcgccctca
     2581 cattaattat ac
//
LOCUS       DAKTLH010000225         2453 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.231, whole genome shotgun sequence.
ACCESSION   DAKTLH010000225 DAKTLH010000000
VERSION     DAKTLH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.231"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            178..948
                     /gene="fabI"
                     /locus_tag="O0394_002692"
     CDS             178..948
                     /gene="fabI"
                     /locus_tag="O0394_002692"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCY0500709.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(1143..2228)
                     /locus_tag="O0394_002693"
     CDS             complement(1143..2228)
                     /locus_tag="O0394_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCY0500710.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
BASE COUNT          886 a          349 c          414 g          804 t
ORIGIN      
        1 cgtaaatcaa gtttgataca gaaaggacta aatcaaacat ttatcgttgt aatacgttta
       61 aataacttta ttaaaaagtc ataatagtgt taaaatgtat tgacgaataa aaagttagtt
      121 aaaactggga ttagatattc tatccgttaa attaattatt ataaggagtt atcttacatg
      181 ttaaatcttg aaaacaaaac atatgtcatc atgggaatcg ctaataagcg tagtattgct
      241 tttggtgtcg ctaaagtttt agatcaatta ggtgctaaat tagtatttac ttaccgtaaa
      301 gaacgtagcc gtaaagagct tgaaaaatta ttagaacaat taaatcaacc agaagcgcac
      361 ttatatcaaa ttgatgttca aagcgatgaa gaggttatta atggttttga gcaaattggt
      421 aaagatgttg gcaatattga tggtgtatat cattcaatcg catttgctaa tatggaagac
      481 ttacgcggac gcttttctga aacttcacgt gaaggcttct tgttagctca agacattagt
      541 tcttactcat taacaattgt ggctcatgaa gctaaaaaat taatgccaga aggtggtagc
      601 attgttgcaa caacatattt aggtggcgaa ttcgcagttc aaaactataa tgtgatgggt
      661 gttgctaaag cgagcttaga agcaaatgtt aaatatttag cattagactt aggtccagat
      721 aatattcgcg ttaatgcaat ttcagctggt ccaatccgta cattaagtgc aaaaggtgtg
      781 ggtggtttca atacaattct taaagaaatc gaagagcgtg cacctttaaa acgtaatgtt
      841 gatcaagtag aagtaggtaa aactgcggct tacttattaa gtgatttatc aagtggcgtt
      901 acaggtgaaa atattcatgt agatagcgga ttccacgcaa ttaaataata tcattcaaca
      961 gctttgttca cgttattata tatgtgagca aagctttttt gcttttataa taatcggact
     1021 gatggaaaat tatttgatat ttcatttgac tgatttttta tgcacaaaaa aatcgcctca
     1081 aaagctgtag tttcaacttt tgatgcgata gtatcttata ttcttaaaag acatccacag
     1141 aattagtcct taacattact gtttgcttta tcaataatgc gttggcggta tttgaaaata
     1201 ttactaacaa ccgttttaag tacagcatat aatggcactg caatcagaac taatgtaaag
     1261 ccacctaaat ctcctgctgc taaaataaca acgataattg ttaaaggatg gatacttaaa
     1321 gatttaccca ttacatttgg tgtaatgata ttaccttcaa gttgatgtgc aattaatgta
     1381 atgatacaaa cccatataaa tgtagtagga ctatctataa taccgagtat tgctgcaggt
     1441 gcaaatgata accatgaacc taagaaagga attaagtttg cgacaccagc aaatagtact
     1501 aataaaggaa tatatggtaa gtcaataatt gaataaccga tatataagaa tatacctaaa
     1561 ataacactga cagttacttg accttgaatg taagatttta atgtaaagtt taaatcagtt
     1621 aataaatcta cgaaaaatac tttacgttca cctttgaaaa atttagcaac agctgggata
     1681 aatttttcat ggtcttttaa catataaatt aagaagaatg gaaccataat caataagaag
     1741 atggttgaaa ttaatgatgt aatgtactgt aatgaattag ataaaatatt agtaacgcca
     1801 tcacccattg atttaaccat atttgtaatt ctacttgtta catcttcagg taatttatcc
     1861 atttgagcta gtgcgaattt aataatttgc tctgcttctt tttgtagggc aggtgtctga
     1921 ctaattaaat tgttgatatt tgaaatgatg attggtgcaa caaacgcaac aattaagccg
     1981 ataatagcaa acaaagctaa catgattgtt gttatactag cccatcttgg aaaaccgact
     2041 ttttcaagta agttttgaaa aggtagacag atataaaata aaaaaccact aattaaaaat
     2101 ggaagaaata cagaaccgat gattgtagct attggagtaa atacttcgtg cacttccata
     2161 aatagtttga tgagaatgaa cagcataatc agagcgatgc cagttcggaa ccaaaccttg
     2221 tttaacataa acgatgttcc tcctattttt ctaaacttag ttattattta gtatatccta
     2281 aatagcagtg cttaaaaagg ttaatatttt ttaaatatac ttaaattaac cattcacttt
     2341 ctattttaag atatttactt tcaatagttt gtgagacttt tcaatgactc gtaagtctgc
     2401 aattggatca aacagcacca gtttatatga ataacttatt ttaatattaa acg
//
LOCUS       DAKTLH010000226         2361 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.232, whole genome shotgun sequence.
ACCESSION   DAKTLH010000226 DAKTLH010000000
VERSION     DAKTLH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.232"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            19..864
                     /locus_tag="O0394_002694"
     CDS             19..864
                     /locus_tag="O0394_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0500711.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVPVRQLDYFNQESMVEAFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYAARLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNDFQQLVNDQPQTLQSF
                     LQENI"
     gene            883..1242
                     /locus_tag="O0394_002695"
     CDS             883..1242
                     /locus_tag="O0394_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HCY0500712.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSKKTSETFNISVDKLK
                     DKNINLLLKNQGVYNGLIGVLLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            1766..1918
                     /locus_tag="O0394_002696"
     CDS             1766..1918
                     /locus_tag="O0394_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500713.1"
                     /translation="MVPEEKGSITLSKEAAIIFAIAKFKPFKNRIKNNPQKTNPFLKL
                     HENKKS"
     gene            2179..>2361
                     /locus_tag="O0394_002697"
     CDS             2179..>2361
                     /locus_tag="O0394_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0500714.1"
                     /translation="MTKERRTFSSEFKLQMVRLYKNGKLKNEIIRKYDLKPSIISNSI
                     KQHQNTGPFNHQDNLKS"
BASE COUNT          856 a          370 c          394 g          741 t
ORIGIN      
        1 gattaggaga tgaagtgaat gaatattatg ttaacaggtg ctacaggtca tttaggcaca
       61 catattacaa atcaagccat tgcaaatcac atagatcatt ttcacattgg tgttagaaat
      121 gttgagaaag ttccagaaga ttggcgcgga aaagttcctg ttcgacaatt agattatttt
      181 aatcaagaaa gcatggtaga agcatttaaa ggaatggata ccgttgtgtt tattccaagt
      241 attatccatc catcattcaa acgtattcct gaagtggaaa atttagtata tgcggcaaag
      301 cagagcggcg ttgctcatat cattttcata ggttattacg cagatcagca taataatcca
      361 ttccatatga gtccttattt tggttatgca gcacgtctat tggcaacaag tggcattgac
      421 tatacgtatg taagaatggc aatgtacatg gatccactta aaccatattt accagaattg
      481 atgaatatgc ataaactgat ttatccagct ggcgatggtc gtattaatta tattactaga
      541 aatgatattg ctagaggtgt cattgctatt attaaaaatc cagatacttg gggcaaacgc
      601 tacttattat caggctacag ttatgatatg aaagaacttg ctgcaatttt atctgaggca
      661 tcaggcacag aaattaaata tgagcccgtt tcattagaga catttgcaga aatgtatgat
      721 gaacctaaag gctttggtgc attattggca tcaatgtacg acgcaggagc aagaggacta
      781 ttagaccaag aatccaatga tttccaacaa ttagtcaatg atcaaccaca gacactgcaa
      841 tcatttttac aagaaaatat ttaataataa aggagcgtta tagtgaatat catctcaaca
      901 attttaatca tatttgtggc attagagttt ttctatatta tgtaccttga aacgattgct
      961 acaacttcca aaaagactag cgagacattt aatataagcg tcgataaatt gaaagacaaa
     1021 aatattaacc tacttttgaa gaaccaaggc gtatataacg gtttaatcgg agttttgcta
     1081 atatacggtt tgtttatcag cagtaatcca aaagaaatat gcgcagctat tttagtgtat
     1141 atcattggcg ttgctattta tggtggcctt tcaagcaata ttagtatctt tttcaaacaa
     1201 ggcacattgc cagtattggc actcatatca atgctttggt aagtattggt gtttgggggg
     1261 gtggagatgt agtcggaggt ttggaggatt tgagcgaatt gtgtgtggac tttagactca
     1321 gagtatttca tcctaattat ttcaagcaga ggtgacagta gcgttgcctc tgtttcctta
     1381 tagaaaaatt atttaatgaa gaaaacccat atctgattta attttcagct gataaatact
     1441 ccatatatta gaatggctac tttatctatt gcatcaatcc tttaaaacaa aaaacccatg
     1501 atttcgaaat tcccgtatga tggggttcct actctcatgg atcagttaaa taaatattat
     1561 cactatcagt ttattatttc aatattatta acaatatatg tagtcgtaaa aggaaagagg
     1621 acatgagaac ttcggtgttg attggcatta cataacgctt ccaaacatat tatttggtaa
     1681 caataagaaa ctatttacac aatatatttt gtatagtaaa attattttat aatatttaaa
     1741 tccaattgca caaggagtga ttatcatggt accagaagaa aaaggttcta ttactttgtc
     1801 aaaagaagca gctatcatat ttgcaatcgc aaaattcaaa ccatttaaga acagaattaa
     1861 aaataaccca caaaaaacaa atccatttct taaattacat gaaaacaaaa aatcttaatc
     1921 actttattta tagcatttct aatctcagaa atgctataaa ggacaacgcc ctctttcata
     1981 actatataag tcaagaaacc aagacatttc aaacatcttt aaccccttca atagcacatt
     2041 ccctaaaaca taaagcaata acaatgaacg atcaaaattc cttacgtact tatgatgtgt
     2101 cagtgaatcg tctacactta attgaacaaa ttctatgaga atagatattg ttaaattaag
     2161 aaagtagtca attttattat gacaaaagaa agaagaacat ttagttcaga gtttaagtta
     2221 caaatggtta gattatataa aaatggtaag cttaagaatg aaattatacg aaagtatgat
     2281 ttaaaaccct caattatctc aaattcgata aaacaacacc aaaatactgg acccttcaat
     2341 catcaagata atttaaaaag t
//
LOCUS       DAKTLH010000227         2347 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.233, whole genome shotgun sequence.
ACCESSION   DAKTLH010000227 DAKTLH010000000
VERSION     DAKTLH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.233"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(6..620)
                     /locus_tag="O0394_002698"
     CDS             complement(6..620)
                     /locus_tag="O0394_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCY0500715.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFVYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGLFCIILSTLLLRNKLIN"
     gene            complement(637..1707)
                     /locus_tag="O0394_002699"
     CDS             complement(637..1707)
                     /locus_tag="O0394_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCY0500716.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTLIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            complement(1719..2228)
                     /locus_tag="O0394_002700"
     CDS             complement(1719..2228)
                     /locus_tag="O0394_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0500717.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
BASE COUNT          752 a          431 c          303 g          861 t
ORIGIN      
        1 actatttaat ttattaattt atttctaagt aataacgttg ataaaataat gcaaaataat
       61 ccaccaataa tgccggcaat aatatctgtt ggataatgta cacctagata tacacgtgat
      121 atggaaatca ataaaatcat agctgcacat aaccctataa gaataccttt tgaattacct
      181 tgatttaatc gatttaatag atagatacca cttccaaaat atgcagttga tcccatagca
      241 tgaccgctag gaaaactaaa tcctgttata tcaattaaac gcagcaatgt aggtctttct
      301 ctatcgaata tattttttaa tgctggattc aaaattccag ataatgccat tgttaatgca
      361 aaaaataatg cttcaatttt gtggcgcttt aacatgagat atgccacaag aagtaatgaa
      421 atacataaca ttgcccagac ttcacctact ttagtagccc caagcatgat agatgtcgta
      481 ataaagctct ctgatgaata tacaaactca taaacttcat tatcaatcca tttcccaagt
      541 cttgattcgt ggaaaaacgc gataattcca aaaaccaatg taaaaacgat gagcaaagag
      601 atacgtttcc attgactcat aaagcattac cccccattaa ttcaatgcgt ctttaatcat
      661 cttatcaaat aaagcttctt tcgtataact ttgttcatac gatttcatat tattgataat
      721 tcgagttctt tcctgttcca tttcatttag ttcttgtaat aaaatttgtg ctgttaattg
      781 ttcttcatca atcgctttag catatccttt atcagcaaaa tgatttgcat tgtcaatttg
      841 gtcgcctcgg gattgatcta aacctaatgg tactaataac attggtatac gtaatgttaa
      901 gaactcataa atcgcatttg atccagctct acttattact gtatccgtaa ttgctaataa
      961 atctgttaaa tcctctttaa caaattcata ttgtatatat cctgattttt taacttgagc
     1021 atcttttaat cctttaccag ttaaatgtat cacttgatat tgttgtaata atgcatctaa
     1081 gttttcgcga ataatgctat ttaatttttt acttcctaag cttccaccca taacgagtaa
     1141 aactttttta ttttcattaa agcctgttaa ttgataacca ttatgtgcat taccattttt
     1201 taaatcttct cgaattgttg ctccaataaa atcagctttc tctttaggta agtagtttag
     1261 cgtttcttca aatgttgtat atattttctt ggcaaattta agtgctatct tattcgctaa
     1321 tcctggtgtt aagtcagatt catgaataag agttggtata tttaatgatt tggctgcaat
     1381 aacaacaggc acagatacaa atccaccttt tgaaaataat agatcaggtt tttctttttt
     1441 caaaacttta cgagcatcaa gaataccttt caatacttta aatacgtctt tggcattttc
     1501 taaagaaata tatcttctta atttaccact cgaaatagga taatacttaa tttctggtag
     1561 ttgtgattca atcatttctc tttcaatacc atttttagaa ccaatataaa gcgcttcata
     1621 accttgtgat aatgcagttg gaattaaact taaatttact gatacgtgtc caactgttcc
     1681 ccctccggta aatgcgattt tcgtcattaa tattacctct attcttttaa atttttataa
     1741 tatgcataaa atggttcacc tttatcatat ggcggatatt ccattaattg ttcaccgacc
     1801 ttatgaaatc caaatttggc aaataaacct tgtgcaggtt tgtttaacgc aaaggtgtcc
     1861 gttaaaataa cttctgcacc acgtgcttta actacatcaa taacataatt gaataattct
     1921 gtagcagctc ctttatattc tttcgaacca gttaatcgat gaataacaaa ggcgccttct
     1981 ctatttactg gccagtcaat gtcatcatac cattctgctt ggtcttggtc gacaacaata
     2041 aagccataaa ttttgtcatt ttcctctaat acgtacaaat aatctttagc aatatcttct
     2101 tcaaaatgtt ctaaaagtgg gtactgatcg tcccattgct cgttgtcgtg ttctttcatt
     2161 aattcttttg cttcttctac tagatttaag atttgatcta aatctgacat tttacctaga
     2221 cggatcatgt acgtcactcc ttatatcatt atgttaaaca tgtttttcag tacatgttaa
     2281 tcttacacac tattaacatg ctttaaacct ttattatgaa tgaaaaatag taattatcac
     2341 actatgg
//
LOCUS       DAKTLH010000228         2341 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.234, whole genome shotgun sequence.
ACCESSION   DAKTLH010000228 DAKTLH010000000
VERSION     DAKTLH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.234"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..392)
                     /locus_tag="O0394_002701"
     CDS             complement(<1..392)
                     /locus_tag="O0394_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCY0500718.1"
                     /translation="MGLYEELCINNEKIKIEETDQLPNFQPGCYMNGKIYIRRNLSEV
                     RKAEVLYEQLAHHKLTYGNILDQTKWINRKFENYARRHGFTSAVPLHEIVEAHNYGVR
                     NLYELSEYLQLSESYILEAIEQYKKIYG"
     gene            complement(405..734)
                     /locus_tag="O0394_002702"
     CDS             complement(405..734)
                     /locus_tag="O0394_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0500719.1"
                     /translation="MENNKVRKILSENLQELMNDKNIDQRELAEAIGVSQPTVSNWIQ
                     QTKYPRIKRIQQLADYFNVPKSRITESKKDIHQETIAAHFDKEGLTEEEIEEVNRFIE
                     WVRNRDK"
     gene            895..1086
                     /locus_tag="O0394_002703"
     CDS             895..1086
                     /locus_tag="O0394_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0500720.1"
                     /translation="MPEQLSVRKWRLVRDLKQQEVADILGVNAKTVGHWEKDDTNLSN
                     VTVYALAKLYDIEVDQIKV"
     gene            1170..1346
                     /locus_tag="O0394_002704"
     CDS             1170..1346
                     /locus_tag="O0394_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500721.1"
                     /translation="MNEEKLKMILLLLEDIPRDEWNRLVNEVNNQYSYQADKVGLASD
                     NCQQIANNYKHYGF"
     gene            complement(1343..1582)
                     /locus_tag="O0394_002705"
     CDS             complement(1343..1582)
                     /locus_tag="O0394_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500722.1"
                     /translation="MISIHKRLLTQYLDKEIVTSLDLHLINGEVIKVQEHIKDAESKT
                     LHIIHPKDRVVSLDHVLYFDINVKGEKNNDSPYPS"
     gene            1629..1844
                     /locus_tag="O0394_002706"
     CDS             1629..1844
                     /locus_tag="O0394_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500723.1"
                     /translation="MNKKSEGLDIIIPRIFRRDHASVESLTENERRLRKEILGSIKKG
                     DYSYLEINKVFYALDRELQYRANNNKL"
     gene            1869..2132
                     /locus_tag="O0394_002707"
     CDS             1869..2132
                     /locus_tag="O0394_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0500724.1"
                     /translation="MPKIIVPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYN
                     WLKYYRKDNLGVENLYIDYSPTGTLINISKLEEYLIRKHKKWY"
     gene            2144..2305
                     /locus_tag="O0394_002708"
                     /pseudo
     CDS             2144..2305
                     /locus_tag="O0394_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
BASE COUNT          828 a          366 c          380 g          767 t
ORIGIN      
        1 ataccatata tctttttata ttgttctata gcttctagta tgtatgattc actcaactgc
       61 agatactctg acaactcgta caaattacga acaccgtagt tgtgagcttc tacaatttca
      121 tgtagcggta cagctgaagt aaaaccatgt ctacgtgcat aattttcaaa ttttctatta
      181 atccattttg tttggtctaa aatgttgcca tacgtcaact tgtggtgggc aagttgctca
      241 tataacactt ctgctttacg tacttctgat aaattacgcc ttatataaat ttttccgttc
      301 atatagcatc caggttggaa atttggaagc tggtcagttt cttctatctt tattttttcg
      361 ttatttatgc aaagttcttc gtataatccc aatataaaca ccctttattt gtctctattt
      421 ctaacccatt caatgaatct atttacttct tcaatctctt cttcagttaa tccctcctta
      481 tcaaaatgag cagcaattgt ttcttgatgt atatcttttt ttgattcagt aattcttgat
      541 ttcggtacat tgaagtaatc tgcaagttgt tgaattcttt taattcgtgg atatttagtt
      601 tgttgaatcc aattggagac tgtaggttga gaaactccaa tagcttcagc aagttctctc
      661 tgatcaatat ttttatcatt cataagttct tgaaggtttt cagataaaat ttttctgact
      721 ttattattct ccatagttgt ctcctttaat attacttaat gtaatattaa cttaccataa
      781 ccgacattac tttacaatac tttttataac tttttatgaa gaaatataac tttatctgtt
      841 gacaagtaat acaaagtaat ataaagttat acgtgtgaaa gggaggtgga cgacatgccg
      901 gaacaattat ccgtaagaaa atggagactt gtaagggact tgaaacagca agaagtagca
      961 gatatattgg gcgtcaatgc aaagacagtt ggtcattggg aaaaggatga cactaattta
     1021 agtaatgtta cagtttacgc tttagcaaag ttatatgata ttgaggtaga ccagataaag
     1081 gtctaaattt tttgaccttc gtataacttt ttataacttt taggtaatgt aacaaatgga
     1141 cataaacaaa ttaaaaggag gaacaacaaa tgaacgaaga aaaattaaag atgattctct
     1201 tacttcttga agatatacca agagacgaat ggaatcgatt agtaaatgaa gtaaataatc
     1261 aatacagtta ccaagctgac aaggtaggac ttgccagtga taactgtcaa caaatagcaa
     1321 ataactataa gcactatgga ttttaagatg gataaggact atcgttattc ttttcacctt
     1381 taacgttaat gtcgaaatac aaaacatgat ctaaactgac aactctatct tttggatgaa
     1441 tgatgtgtag agttttgctt tcagcatctt ttatatgttc ttgtacttta ataacttcac
     1501 cattaattaa atgtaaatct aaagaagtaa cgatttcttt atctaaatat tgagtgagca
     1561 atctcttatg tatacttatc acctccttag gttgataaca acattataca cgaaaggtgg
     1621 aacaacaaat gaacaaaaaa tcagaagggt tagacataat cataccaagg attttcagaa
     1681 gagatcatgc gtcagtagaa tctttaacag aaaatgaacg tcgactaaga aaggaaatat
     1741 taggaagtat taaaaaaggt gattacagct acttagaaat aaacaaagtt ttctatgcat
     1801 tagatagaga acttcaatac agagcgaata ataacaaact ttaacattta tcgaaaggag
     1861 tgatagagat gccaaaaatc atagtaccac caacaccaga aaacacatat agaggcgaag
     1921 aaaaatttgt gaaaaagtta tacgcaacac ctacacaaat ccatcaattg tttggagtat
     1981 gtagaagtac agtatacaac tggttgaaat attaccgcaa agataattta ggtgtagaaa
     2041 atttatacat tgattattca ccaacaggca ctctgattaa tatttctaaa ttggaagagt
     2101 atttgatcag aaagcataaa aaatggtatt aggaggatat taaatgagcg acacatataa
     2161 aagctaatta atagcagtac tgtgcttcac agtcttagcg attgtgctta tgccattgct
     2221 gtacttcact acagcatggt caattgcggg attcgcaagt atcgcaacat tcatatacta
     2281 caaagaatac ttttatgaag aataaaaaaa ctgctacttg cgtcaacaag taacagtgac
     2341 a
//
LOCUS       DAKTLH010000229         2317 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.235, whole genome shotgun sequence.
ACCESSION   DAKTLH010000229 DAKTLH010000000
VERSION     DAKTLH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.235"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..116
                     /locus_tag="O0394_002709"
     CDS             <1..116
                     /locus_tag="O0394_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0500725.1"
                     /translation="QNYYKYRKFGILPETEYEINKVKEIEQYVKEQEEAEQ"
     gene            127..627
                     /locus_tag="O0394_002710"
     CDS             127..627
                     /locus_tag="O0394_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0500726.1"
                     /translation="MNFEEKLSEMYNKIANEINEMIPVEWEKVYVIAYVDDGGGQVIF
                     YYTKPRNDELYYYSSIVEDYNVLEEIFDDLWMELYRSFKKLRNIFKEESLEPWTSCEF
                     DFTKEDKLKVSFDYIDWKNTEFDQLGQENYYNYRKFGIIPEMEYEMEEVKQIEQYIKE
                     QEEAEQ"
     gene            638..1133
                     /locus_tag="O0394_002711"
                     /pseudo
     CDS             638..1133
                     /locus_tag="O0394_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
     gene            1144..1635
                     /locus_tag="O0394_002712"
     CDS             1144..1635
                     /locus_tag="O0394_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0500727.1"
                     /translation="MTFEEKLSEMYSEIANKIISMIPVEWEKVYAMAYIDEECGEVFY
                     NYTEPSSDELFYYTSVIKKYNLLKSSFMDSVYELHDQFEELREVFIEEGLEPWTSCEF
                     DFTKEDKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPETEYEMEEVKEIEQYIKE
                     QEE"
     gene            1850..>2317
                     /locus_tag="O0394_002713"
     CDS             1850..>2317
                     /locus_tag="O0394_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCY0500728.1"
                     /translation="MTTKENIDILRKPGAQALSLASLFMILFSCLTFFFGLDYERFPN
                     YLKITTIIELIIIIISLLQWIRFIDFEKESAQKYKKIYARFLVIINVLTTITAVFATC
                     NLYYFVAVQNHYDLFNYWLMGTISIIISYLLLVIGGMFTLLKLPKVTKRWGGKT"
BASE COUNT          940 a          225 c          431 g          721 t
ORIGIN      
        1 gacaaaatta ctataagtat agaaaatttg gaattttacc agaaacggaa tatgaaatta
       61 ataaagttaa agaaatcgag caatatgtta aagagcaaga agaagctgaa caatagaggc
      121 gataacatga atttcgaaga aaaattaagt gaaatgtaca ataaaattgc aaatgagatc
      181 aatgaaatga taccagtaga gtgggaaaag gtatatgtaa ttgcatatgt ggacgatgga
      241 ggtgggcaag ttatatttta ttataccaag cccagaaatg atgagctgta ttattattcg
      301 agtatcgttg aagattataa tgttttagaa gaaatctttg atgatttatg gatggaactt
      361 tatagatcat ttaaaaaatt aagaaatata tttaaagaag aaagtcttga accatggaca
      421 tcatgtgaat ttgactttac aaaagaagat aaattaaaag tttcatttga ttatattgat
      481 tggaagaata cagaatttga tcaattaggc caagaaaact actataacta tagaaaattc
      541 ggaattatac cagaaatgga atatgaaatg gaagaggtta aacaaatcga gcaatatatt
      601 aaagagcaag aagaagctga acaatagagg cgataacatg attttcgaag aaaaactaaa
      661 tgaaatgtac aacgagattg cgaataaaat tagtagcatg ataccagtag aatgggaaaa
      721 ggtatataca atggcttata tagatgatgg aggaggtgaa gtattcttta attatactaa
      781 acagcgatga attgaattat tacaccgata tacctaagga gtataacatt tctgtgcaag
      841 tatttgatga tttatggatg gatttatatg atttgtttga ggaattaaga aatttattta
      901 aagaagaagg actagaacca tggacatcat gcgaatttga ttttacaaga gaaggtaaat
      961 taaaagtttc atttgattat attgattgga taaattcaga atttggtcaa gtaggtcgac
     1021 aaaattacta taagtataga aaatttggaa ttttaccaga aacggaatat gaaattaata
     1081 aagttaaaga aatcgagcaa tatattaaag agcaagatga agctgaacta taggggcgat
     1141 aatatgactt tcgaagaaaa attaagtgaa atgtatagcg agattgcgaa taagattatt
     1201 agcatgatac cggtagagtg ggaaaaagta tatgcaatgg catatatcga tgaagaatgt
     1261 ggagaagtgt tttacaatta tactgaacca agcagtgatg aattatttta ctatacaagt
     1321 gtgataaaga agtataattt attgaaatca agctttatgg attcagtata tgagttgcat
     1381 gatcaatttg aggaactaag agaagtattt atagaagaag gtcttgaacc atggacatca
     1441 tgcgaatttg attttacaaa agaagataaa ttaaaagttt catttgatta tattgattgg
     1501 ataaatacag agtttgatca attaggccgt gaaaattatt atatgtacaa aaaatttggt
     1561 gttttaccag aaacggaata tgaaatggag gaggttaaag aaatcgagca atatattaaa
     1621 gagcaagaag aatagacaat cttaatgtaa gattaaacaa taaagctttg tttagtcttt
     1681 ttagcgttta agtaaaaaac aatagagacc ataaagttgt catcaaataa taatataaag
     1741 ataattttag gcttttatac ttttaatcga aaagaaaaga atctaagtac ttataacttt
     1801 aaatacttaa tatgctatta caaaatcaat gtataggaga taggtaaata tgactactaa
     1861 ggaaaatata gacattcttc gaaagccagg tgcacaagcc ttaagtttag catcattatt
     1921 tatgatactt ttttcatgtc taactttctt ttttggttta gattatgaaa ggtttccaaa
     1981 ttatttaaag ataacgacaa ttatagaatt aataattatc ataattagtt tacttcaatg
     2041 gattagattt atagatttcg aaaaggaaag cgcacagaaa tataagaaaa tatatgcccg
     2101 atttttagtt attataaatg tgctaactac tatcaccgca gtatttgcaa catgtaacct
     2161 ttattatttt gttgctgtac aaaatcatta tgacctattc aattattggt tgatgggtac
     2221 gatttcaatc ataattagtt atttgttatt agtaattggc ggaatgttca cgttattaaa
     2281 attacctaaa gtaacaaaac gttggggtgg taaaact
//
LOCUS       DAKTLH010000230         2311 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.236, whole genome shotgun sequence.
ACCESSION   DAKTLH010000230 DAKTLH010000000
VERSION     DAKTLH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.236"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            61..1050
                     /gene="trpS"
                     /locus_tag="O0394_002714"
     CDS             61..1050
                     /gene="trpS"
                     /locus_tag="O0394_002714"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCY0500729.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
     gene            complement(1092..1973)
                     /locus_tag="O0394_002715"
     CDS             complement(1092..1973)
                     /locus_tag="O0394_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0500730.1"
                     /translation="MQNKSKSPFKIAFSKFIHNKIAMLSVIFLLIITIVSIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPWIWMPPAIMITLTILSI
                     NFVGEGIKDAFNPRGRR"
     gene            complement(1985..>2311)
                     /locus_tag="O0394_002716"
     CDS             complement(1985..>2311)
                     /locus_tag="O0394_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0500731.1"
                     /translation="NSKKDYVLTARSKGLSMNKIYNKHILRNSLIPIITFLGADIVSI
                     LGGAVITETIFSYNGIGKLFLESVIGQDYPLMMALTLFFSFLGLLGNLISDITYGFID
                     PRIRSN"
BASE COUNT          849 a          388 c          350 g          724 t
ORIGIN      
        1 tttaagaaat actgttataa tgataattat taaaatatta ctaagaaagt aggcatttaa
       61 atggagacat tattttcagg catccaacct agtggaattc ctactattgg aaattatatt
      121 ggcgcactaa aacaatttgt tgatgtgcaa aatgactatg attgttattt ctgtatcgta
      181 gatcaacatg caattacaat gccacaagat cgtttaaaat tacgtaaaca gaccagacaa
      241 ttagcagcga tttatttagc ttctggtata gatccagaca aagcaacatt gttcatacaa
      301 tctgaagtcc ctgcacacgt acaagcagga tggatgttaa ctacgattgc ttctgttgga
      361 gaattagagc gtatgacgca atacaaagat aaagctcaga aagcagttga aggtatacct
      421 gctggtctat taacatatcc acctttaatg gcagctgata ttgttcttta caatactaat
      481 atcgttccag ttggagatga ccaaaagcag catatcgaat tgactcgtaa ccttgtagat
      541 agatttaata gtcgctataa tgatgtgctt gtgaaacctg aaattcgtat gcctaaagtt
      601 ggtggacgtg tcatgagttt acaagaccca acaagaaaaa tgagtaagag tgatgataat
      661 gctaaaaact tcatttcatt attagacgag ccgaatgttg cagctaaaaa aattaaaagc
      721 gcagtaactg attcagatgg tattattaaa tttgatcgtg acaacaagcc aggtataaca
      781 aatttaattt caatatacgc tggattaaca gacatgccaa ttaaagatat tgaggcaaaa
      841 tatgagggcg aaggttatgg taaatttaaa ggtgaccttg ctgaaatagt taaagcattt
      901 ttagtagaat tccaagaaaa atacgaaagt ttctataact cagataaact tgatgatatt
      961 ttagatcaag gtagagataa agcacacaaa gtttcattta aaactgtcaa aaaaatggaa
     1021 aaagcgatgg gtttaggacg taagagataa aataaagagg tactagaatt aactagtgcc
     1081 tcttttatta tttaacgtct acctctagga ttaaaagcat ctttaatccc ttcccctaca
     1141 aagtttatac ttaatatagt taatgtaatc attatagcag gtggcatcca tatccacggt
     1201 ttaccactta taacatcccc ttcttgagca tcactcaaca tattacccca agatggtatt
     1261 gctttactaa ttccgaatcc taaaaagctc aatcctgatt ccactacaat cataccggca
     1321 aataacaatg ttgcttgtac gataactaca cttaatatat tcggcaaaag atgtttcaaa
     1381 ataattttat atgtgggtgt accaattgat tttgctgcca aaaagtattc attttctttt
     1441 tcttgaagta ctttaccacg aacaagtctt gcaatacctc cccaacttaa tacgataata
     1501 actagaacaa gaattatggc agatccataa ggatttttaa ttttatctcc aagtgcagca
     1561 tttaatacaa ttgcaaatat taaaaatgga aataacataa caaattcggt tattctcatt
     1621 aataatgtat caacaatacc tccaaaataa ccagaaatca ctccaactgt aattccaatc
     1681 aaaagcattc ctattgtaga tgtaattcca atggataatg aaatacgacc tgcatataac
     1741 aaacgactaa agttatccct accaccagag tctgtaccaa gaatgttttg tgctgtcatt
     1801 tcacctttta tatttaataa atcttgttgg ttcactggaa aaggagctat taatggcgct
     1861 ataattgata caatagttat gattaataaa aaaataaccg ataacattgc aattttatta
     1921 tgaataaatt tagaaaatgc aattttaaaa ggcgattttg acttattttg cattctaatc
     1981 ccccctagtt acttctaatt cttggatcta taaatccata agtaatatca gaaatcaaat
     2041 tacccagtaa acctaaaaat gagaaaaata acgttaatgc catcattaat ggatagtctt
     2101 ggcctattac cgattctaaa aataatttac cgataccgtt atatgaaaag atagtctccg
     2161 taatcacggc tccacctaaa atacttacaa tatcagcacc taaaaatgta ataataggta
     2221 ttagagaatt tctcaatata tgtttattat aaattttatt catagataat ccttttgacc
     2281 ttgccgtcaa tacataatct tttttagaat t
//
LOCUS       DAKTLH010000231         2286 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.237, whole genome shotgun sequence.
ACCESSION   DAKTLH010000231 DAKTLH010000000
VERSION     DAKTLH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.237"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(190..720)
                     /locus_tag="O0394_002717"
     CDS             complement(190..720)
                     /locus_tag="O0394_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0500732.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNTLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            complement(859..1380)
                     /locus_tag="O0394_002718"
                     /pseudo
     CDS             complement(859..1380)
                     /locus_tag="O0394_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1388..1543)
                     /locus_tag="O0394_002719"
     CDS             complement(1388..1543)
                     /locus_tag="O0394_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500733.1"
                     /translation="MMVKKLLKAIKQNFLYEQSNITFKMIVFGYKRLSKMLFQFEINH
                     INFLWEG"
     gene            1979..>2286
                     /locus_tag="O0394_002720"
     CDS             1979..>2286
                     /locus_tag="O0394_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HCY0500734.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     "
BASE COUNT          833 a          364 c          266 g          823 t
ORIGIN      
        1 tggaattata cactaaaatt gcattatagc aattaatttg tatcgatatt ttattatcca
       61 caataatact ttactaacaa acattttatt tattgctatt ttaagaatta caaacgacaa
      121 cgtacgattt gattgcaaac attttttatt attaatatga actctaccta atgtaatcct
      181 agcttgaaat catatttttt caaaagcaga tgtgtaattt atggtacctg tttttcccgc
      241 taaactgttc acttttaatt ctttaattaa aaacgcttcg tctggtatac catcatatgg
      301 tggataaatg tcgtacatac ttgcgcgttg atatcctaga ttggcataat acgtcggcca
      361 tcctaataca ctgataaaat tatatccttt taatattgct tctcgttcta atgcttgaat
      421 caatcgcttc ccaatacctt tattttgatg atgaatatca acagatacag gtgctaacac
      481 taatccaatt tcccgttgtg cctcgttatc aagataaact tcacttagta aaccgtgccc
      541 tacaacttca ttttgaagaa cagctactaa ttctaaggta ttgtcatacg tatcacttag
      601 acgaatttgg tctactagct ctgattcatt accataacca tgttcactat tttcaaatgc
      661 cgttctaatt aactggtcca cttgagcaaa gtcatgttga tgtatttgtc taatttgcat
      721 ctttgcccct ccttttagtt ctatcttata gaaaaatgca ttacaacata aagtaatatt
      781 ttatagtaat gacgccatta tttcattaaa attgaatgat atttttcgca ataaacaact
      841 accctcttaa atcaattttt agataaactg caaatcataa atttctttat tattataagt
      901 attaaaacgg tcgatttgac cttttaatat aacttcctat tgtttgcgac ttacattatg
      961 ataataagtc atacgtacat tcaaatcagc ttcatgacca ttgttctcaa tcgttctttc
     1021 taattcttcg tacacatttt tattaattgg taacatgtac ccttctttac taggtaaaat
     1081 atgcacgtat tttttctgaa tgactttatc cttccccaat aaaacctcta ttgcaatttt
     1141 tttagcagca ccgccaccta aattatatag tttaatataa tctttatgat gatgctcttc
     1201 tgttgccgaa ttcattatat taagttgatc ctcctccctt tctaataaaa tttggttaaa
     1261 acctaaagct ggcagaaagc taattttcat ttgataaagt tgaactgaaa ctgatacaaa
     1321 ataaaataaa gccataataa atgttccaat agatcctata gctgaaataa tgttaatcat
     1381 taagatatca accctcccat aagaaattta tatgattaat ttcaaactga aaaagcattt
     1441 tacttaatcg tttatatcca aaaacaatca ttttaaacgt tatattcgac tgctcatata
     1501 aaaagttttg ctttatagcc ttaagtaatt ttttaaccat caagtacgat tattattacg
     1561 gcaatctatt tgtattgact aaaaaataca ataacgcttt taagagctcc catctttgac
     1621 cagtattttc caaattaata acacgtcttc ttcaatacat atttatctca tacaaataag
     1681 ttgatttttt tatttttaca aacgtaattt cttaacaaat caaaatttta catttaaatc
     1741 tttcgatata ttacaagttt ttttaaaaaa gtaatatatt tctatcaata aaagtaatat
     1801 aatattagta attatagttt cattaattta ttgcaacaat acataggatc attattacaa
     1861 gaattattta atatcatctt agaatctcac aaaatatcaa ctttgtttaa ttactcaaaa
     1921 tgataagtca tttagagttt ttaaaataaa tttttgcgaa ataaaggaga catgttaaat
     1981 gaaaaaagta atggggatat tattagcaag tacacttatc ttaggtgctt gtggacatca
     2041 tcaagatagt gcaaaaaaag agagcactag tcacaaaaag aaagaaaatg acaatgaaga
     2101 attaaatgaa gaacttaaag aatttaaaag caaaaaaaat atggatataa aaattaaagg
     2161 cgatactatt gttagtgaca aatttgaagc taaaataaaa gaaccgttta tcatcaatga
     2221 aaaagatgag aaaaagaaat atatcgcttt taaaatggaa attactgcta aaaaagacga
     2281 taaaga
//
LOCUS       DAKTLH010000232         2280 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.238, whole genome shotgun sequence.
ACCESSION   DAKTLH010000232 DAKTLH010000000
VERSION     DAKTLH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.238"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..1544)
                     /gene="bshC"
                     /locus_tag="O0394_002721"
     CDS             complement(<1..1544)
                     /gene="bshC"
                     /locus_tag="O0394_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HCY0500735.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGT"
     gene            1739..2179
                     /locus_tag="O0394_002722"
     CDS             1739..2179
                     /locus_tag="O0394_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0500736.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
BASE COUNT          725 a          392 c          324 g          839 t
ORIGIN      
        1 gtcccaaaat cattcaaaat ttgaagtgga ttccatattc tttcttgtaa tcctcccatt
       61 ggatggagtg tttcttgaat ttctctaaat tgcttcatac tgatgtcgtt ttctctttca
      121 atgtttaaaa gataacgttt taacaaataa tcatactgtt gtatatgaat ttcattattt
      181 ttattcacaa ggttaatatt attttgattc cccgccactt catctaataa gtctttgttt
      241 agacgacgct gttgttcaat cataccttct accttttcaa taaattgatg tgatgcttgt
      301 tctctaataa acttacttct ttctccttca acaccatcga ctaacacttt ttctaatgga
      361 atattgtatt tcgaaagtaa tttttctata cggtcattta aataagtaat tctaagcctt
      421 ggcatcacga taggcatttc aacatcaaat agttcaaata catcttttag ttcagcccag
      481 tacttaattt cactcggtcc tccaacaaat gccaccgtgt taaataacca ttcttccatt
      541 aatggtcttg tcactacatt attagaaaat aattcaggtt gattttccgc aatatttata
      601 atttcttcct ttatatatgt cttatctgtt ttatttaatt taaaatgctt accatcatac
      661 gaaactaatt gacgcatatt ttcatcatgt aagaataaat gaacatttgt atctgtttgt
      721 atcatcgcat tcaagccttg attttgagta cgttgttgtg ttgctctaaa agcatcatca
      781 agcaactgat gttttttcaa aatcttttta aacataggcg cttccatttt tcttaacggc
      841 tcaaactgcg catctataaa tagaacgcca tatgctttaa atgtttcatg cagtagtgct
      901 ttaaacatat cagtccacga gtcatattgg tcaataattc tgtcacaaat ctccagtaga
      961 ccttgtgtat gaacagtttc tttcatatga atgaacatcg tttttaaagt ttgtttcaac
     1021 tcagccttat caggataata tctagagaca gtcgtctctg gcatctccat tgtatgatat
     1081 ttaaccttat gcagcgaccc atgattttcg ttataaacaa atgtatgatt cacttcatcg
     1141 aaatcatgat cttctcctgc aatccaaaaa actggtacta cttgttgctt atgtgtatcc
     1201 gttaattcct tagataaagt aatgattgaa aatattttat ggaatgtata caatggtccc
     1261 ccgaaaagcc ctgcttgttg tccaccaatc acaacttttg aaccattagc taaatgttgt
     1321 atgtttaatt cttgttcact tgaaagcttt aaatcactca tatattcacg aataacattc
     1381 gctaatgccg cttctcttcc attattttct ttagacatcc ttttttcaaa actagtttgt
     1441 tgagctgcat catattgaaa taatcctgtt attacagggt cactgctctt tatttttggt
     1501 ataaactgat ctttttcatt taaactaact actttacagt ccatgttttt tctccttaag
     1561 tacgcgatta caaactatag tataaagtct ataccggtga ttgacaattt tacggcttga
     1621 aaatcaattt aatcatggaa aatttataat attcattgtt ttacattttc aaatcaatga
     1681 aaaacacaag tggtttaatg tataataata gtagtaaaca aataaggggt agataaatat
     1741 gagtgaaatc aaacgtcttg aaattaatta caaaactgac gaattattcg aaaactttag
     1801 agcgtttggc aacaaagact tatacatggt caatgagtta aacggtcaaa tgattgatgc
     1861 aagttcagat tcaccatttt atggcatatt tgtcggagat caattaggag ctagaatggc
     1921 attactaaaa aaaggtgatg tcgaagaaat ctacttccca gattttgaag attatatatt
     1981 attatggaag ttagaagtat taccaaaata tcaaaacaga gggtacgctt cagaattgat
     2041 tgattttgca aagagtttca atatgccaat taaagccatt ggcagaaatg attctaagga
     2101 tttcttttta catcatggat ttacagatgt ggaagctaaa aatatagagg gacatgatgt
     2161 cttattgtgg aaaccataag ataataatat tcgacactac gagcatgaaa atgcatcttt
     2221 tcgtagtgtc ttttttacaa ttactttctt aagctaatat aagtaaatca ttttcaaatt
//
LOCUS       DAKTLH010000233         2268 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.239, whole genome shotgun sequence.
ACCESSION   DAKTLH010000233 DAKTLH010000000
VERSION     DAKTLH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.239"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..330)
                     /locus_tag="O0394_002723"
     CDS             complement(<1..330)
                     /locus_tag="O0394_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0500737.1"
                     /translation="MLLCESKIINKNPKYRIIKYNDEYLMVDIISTWISLFFPFINWF
                     IPKEYVKISREEFENLNIVKPAKKNVFWPVAGSSALLGVALRKYTHLLDIQLDKKLVI
                     AICCITFI"
     gene            complement(354..599)
                     /locus_tag="O0394_002724"
     CDS             complement(354..599)
                     /locus_tag="O0394_002724"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500738.1"
                     /translation="MYYLKLTRWKNDGLAGFTAGSISAILVYWTNQKNEFGIKDKNDW
                     IGHKLDVGIDAVEKSAEKTVDGVENVMVKLQKVFLII"
     gene            complement(565..1200)
                     /locus_tag="O0394_002725"
     CDS             complement(565..1200)
                     /locus_tag="O0394_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCY0500739.1"
                     /translation="MLCESKVINKNPKYRVIKYGDEYLMIDLVSTWLTLFLPMINWLI
                     PKKYVKISKKEFDDLNIVKPVKNKAFWPVAGSTILFGVTFRKYIPSLNIQLEKNMVIV
                     ICCAIFLGVLILFLFLNRKLRLEIYNNNSSKGKIILFPSLKNFCFTIFYYFLFGGLSI
                     MALSMLLTLNPQNIIGFIGWLVMTAGFFLLNMSSIIDKKIYVLSKTNTVEK"
     gene            complement(1237..1878)
                     /locus_tag="O0394_002726"
     CDS             complement(1237..1878)
                     /locus_tag="O0394_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0500740.1"
                     /translation="MLCETENINKNPKYRIIKYKDEYLMIDLVSTWLALFFPMINWLI
                     PKKYVKISEKDFETLNIVKTAKINSFWPVAGSTVLFGVMLRRYSHLFIVKYEYSIVIL
                     ICCIIILGIFLFFLYLNQKLKLQIYNENKNKSNKIIIFPTLKSLCLSIVLYIYLGGGS
                     FFTIYMLLTIEVQNIILFITLFVIFLFFLFLNMCSLYDNKVHVLFKSNGIEKF"
     gene            complement(2002..>2268)
                     /locus_tag="O0394_002727"
     CDS             complement(2002..>2268)
                     /locus_tag="O0394_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0500741.1"
                     /translation="KLKLKVFDTNIEKNKRVILIPTFKLGCFLVFGYIFAGSFSIFSL
                     IALMTIEPQNIIIFIYWIMMTMLFFLLNMTSIGNEKVRVIMKNN"
BASE COUNT          842 a          349 c          248 g          829 t
ORIGIN      
        1 tataaatgtg atgcaacata tggcaataac taatttttta tcaagttgaa tgtcaagtaa
       61 atgtgtatat tttcttaatg caactcccag caaagcagag ctacctgcaa ctggccaaaa
      121 aacattcttt ttagcaggtt taacaatatt taagttttca aactcttctc tactaatttt
      181 gacgtattct tttgggatga accaattaat aaaaggaaaa aataaactaa tccaagtgct
      241 tattatatcg accattaagt attcatcatt atatttaata attctatatt ttgggttttt
      301 attgatgatt ttagattcgc atagcaacat ttaccagctc catttcttag ggcttatatg
      361 attagaaata ctttttgaag cttcaccatg acattttcaa caccatctac tgttttttct
      421 gcagattttt ctacagcatc tataccaacg tctagtttat gtcctatcca atcgttttta
      481 tcttttattc caaattcatt tttttgattg gtccaatata caagtattgc cgaaatagaa
      541 cctgcagtaa atccagctaa accatcattt ttccaccgtg ttagttttag ataatacata
      601 aatttttttg tcaataatcg atgacatgtt taacagaaag aaacctgcag tcattaccaa
      661 ccaaccaata aagcctatta tattttgagg atttaaagtt aataacatac ttagagccat
      721 tattgaaaga ccgccaaata aaaaataata aaatattgtg aaacaaaagt tttttaatga
      781 aggaaataaa attattttcc ctttactaga gttattatta taaatttcca accttagctt
      841 acgattcaga aataaaaaaa gtattaaaac acccagaaat attgcacaac atattacaat
      901 caccatgttt ttctctaatt gaatattaag tgaaggaata tattttctaa acgtaactcc
      961 gaacaaaata gtactacctg caactggcca aaaagcttta tttttaacag gtttgacaat
     1021 gtttaaatcg tcaaattctt ttttactgat tttgacatat ttttttggaa ttaaccaatt
     1081 aatcatagga agaaataaag ttaaccaggt acttactaaa tcaatcatta aatattcatc
     1141 accatattta ataactctat acttaggatt tttgttgata actttagatt cacatagcaa
     1201 tatttatcac taccattcct ttgtttatta ttcctattaa aacttttcaa ttccatttga
     1261 tttaaataat acatgaactt tattgtcata tagtgagcac atatttaaaa ataagaaaaa
     1321 caaaaatatt acaaacaaag ttataaataa tattatattt tgaacttcaa tcgtcaataa
     1381 catataaata gtaaagaatg aaccaccacc taaataaata tataaaacta ttgataaaca
     1441 aagactcttt aaagtgggaa atatgattat cttattgctt ttatttttgt tttcattata
     1501 gatttgtaac tttaactttt gatttaaata taaaaaaaac agaaaaatac ctagtattat
     1561 gatgcaacaa attaaaatta ctatactata ttcatattta acgataaata aatgggaata
     1621 ccttcttaac ataacaccaa ataagaccgt acttcctgcc actggccaaa aagaattgat
     1681 tttagctgtc ttcacaatgt ttaaagtttc aaaatctttt tcgctgattt tgacgtactt
     1741 ttttggaatc agccaattaa tcattgggaa aaagagtgct aaccatgtac ttactaaatc
     1801 aatcatcaaa tattcatctt tgtatttgat aattctatat ttgggattct tattaatatt
     1861 ttcagtttca caaagcaatg tctccacttc ttttcaattg gttttatatt gatacacttt
     1921 catatacatt agaagtcatt taaattctac tagcgtctta aaaggattgt gtcgacaaca
     1981 tttagaatat tttaaatgta atcaattatt tttcattata acgcgaactt tttcattacc
     2041 tatcgaagtc atatttaaca aaaagaaaag cattgtcatc ataatccaat aaataaatat
     2101 tattatattt tgaggttcga ttgtcataag ggcaattaat gaaaatattg aaaaacttcc
     2161 agcgaaaata tatccgaaaa ctaaaaaaca gccaagttta aacgttggta ttaatataac
     2221 tctcttattt ttttcaatat ttgtatcaaa cacttttaac ttaagctt
//
LOCUS       DAKTLH010000234         2239 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.240, whole genome shotgun sequence.
ACCESSION   DAKTLH010000234 DAKTLH010000000
VERSION     DAKTLH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.240"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..718)
                     /locus_tag="O0394_002728"
     CDS             complement(<1..718)
                     /locus_tag="O0394_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0500742.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYWSSLKNNILNIYEKAQFLSIND
                     VHTSIKLIEMNQGISFLPLYI"
     gene            828..1973
                     /locus_tag="O0394_002729"
     CDS             828..1973
                     /locus_tag="O0394_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCY0500743.1"
                     /translation="MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGM
                     CTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHI
                     KEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDGTRTSREKLKEYMEIISKYQVRT
                     VTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIE
                     TTFLRIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQISENKPI
                     IGENIFKHESGIHQDGTKKNINMYQYLVPSDLGLKNSQVVQFPISNISSKKILHNKFK
                     SIVNTEEIDENISFYKLVNKFSPEVAPEDTVDLLQIIKRRSKDGNFK"
     gene            1957..>2239
                     /locus_tag="O0394_002730"
     CDS             1957..>2239
                     /locus_tag="O0394_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HCY0500744.1"
                     /translation="MEILNDSVKSFEKLYLQLQDGFPVIVPTDTNYNLCSLPNNDLCI
                     DKIFEYKKRSKDKPLSLFIDKPEDWKLYGDNQNTEIVDKLVEIFWPGPLN"
BASE COUNT          869 a          257 c          346 g          767 t
ORIGIN      
        1 ttatataaag aggcaggaat gatatacctt gattcatttc gattaattta attgaagtat
       61 gcacatcatt gatagataga aattgtgctt tttcataaat atttaaaata ttatttttaa
      121 gtgatgacca atattctgga tggttatcac ttattatttt gtatttttca aataaagatg
      181 cctcagttag aagatgatta ttctctttat tgggagcaat caatacaatt ttaccttcgc
      241 atactttttc agaatgaact tctcttagtt taggttgatt tctgctaatc ccgatgtcat
      301 acgtatgatt attaatatct ttttcaatat tttcattttt gacatgaaga gaaacatcga
      361 taaaaggatg ctcgttaaag aaagatttta aaaatttggg cataatgaat gtcgcgatat
      421 atgaagacac gacaacattt aatttcgatt gaaacatcgt ttttttaagt tggatatgtt
      481 tgatgccact ttcatattgt tcaataaaac tttgcgcaat tggaagaaat gtatgaccat
      541 cttcagtcaa gataatttga tttttataag tttcaaatag tttcacattg agatgctgtt
      601 ctaaattttt aatttgctta tgtatagaag gtatagtgag attaatttct tcactagcta
      661 atcgatagtt taacgtcttc gctaaaatga caaatgtata gtaccaatct aaattcatga
      721 acttacctct ttcaaaaaag ttaataattc attagataat atttaatttt tattgcataa
      781 aaataatatt aatattatat cagaggttat cgagggggtg aagtaatatg attcgaattc
      841 aagataatac aataagggat ggtatgcaac aaagtaatgt tcgaaaaagt ctaattataa
      901 aaaaagaagt attgaaacaa attaacaagt taaatataaa ttctgttgaa gtaggcatgt
      961 gtacaactat cgaggatgaa tttaatattc atcaattcag agacatttta agtcctgaaa
     1021 aagaattagt agtattgact aggcttaatg aaaaagaaat aaaaaaaata gtcaaattga
     1081 aaattcataa tttagtggta aaaatactat tgccaatatc tgacttgcat ataaaagaaa
     1141 agcttaattt ttcaaataaa tattatattc agaaaatcaa agactgcttg gatatattaa
     1201 agaaagataa aaaaggagta gatatttgtt ttgaagatgg aacaaggact tctagagaaa
     1261 aattgaaaga atacatggaa attatttcaa aatatcaagt tagaacagtt acatttgcgg
     1321 acactgtagg atgttcgaca ccattagaat acggagatat ttttaattac tttgtaaaaa
     1381 aatattctaa cataattttt tctgctcatt gtcataacga tctagggttg gctactgcaa
     1441 atacattagc tgcaatttta aatggtgcaa agcaaataga aactacattt ttgagaattg
     1501 gtgagagagc gggtaatgct cctattgagg aaataattac tattttgaca aaaaaacaaa
     1561 tagaaagtac ggaattcact ttacccgacg tatataaaac tagtattaat atttctaaaa
     1621 ttctcgattt tcaaatatca gaaaacaaac ctataattgg tgaaaatata tttaaacatg
     1681 aatcaggaat tcatcaagat ggtactaaaa aaaatataaa tatgtatcaa tatttagttc
     1741 ctagtgattt aggattgaaa aattcacaag ttgttcagtt tccaataagt aatatttcta
     1801 gtaagaaaat cttgcacaat aaatttaaat caatagttaa cactgaagaa attgatgaaa
     1861 atatttcttt ctataaactt gtgaacaagt tttcacctga agtagcacct gaagatacag
     1921 tggatttact tcagataata aaaaggagga gtaaagatgg aaattttaaa tgattctgta
     1981 aaaagttttg aaaaacttta tttacaatta caagatggtt ttccagtgat tgttcctact
     2041 gatactaatt ataatttatg tagtttacct aacaacgatc tttgtatcga taaaatattt
     2101 gaatataaaa agcgatcgaa agataagcca ttatcattat ttattgataa gccagaggat
     2161 tggaaattgt atggagataa tcaaaatacg gaaatagttg ataaactagt tgaaatattt
     2221 tggcctggac cattaaata
//
LOCUS       DAKTLH010000235         2213 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.241, whole genome shotgun sequence.
ACCESSION   DAKTLH010000235 DAKTLH010000000
VERSION     DAKTLH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.241"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            54..488
                     /locus_tag="O0394_002731"
     CDS             54..488
                     /locus_tag="O0394_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500745.1"
                     /translation="MKKVIGLLLVSTLALTACGEKEKPKKEENKKSQTQNHKDSKPKT
                     QQEKMKKVEDKNPPNNSIQNNSNNQNQSQNNQLNNNSDPSNNTPANINENDSQNTNLN
                     DEYVVSPGWTKDEQAKAFEEYKKGKEEEARAGASAVPGANIN"
     gene            517..912
                     /locus_tag="O0394_002732"
     CDS             517..912
                     /locus_tag="O0394_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500746.1"
                     /translation="METNKTIDLMNYVEFPKRYTEAKGKLVAQPITTINSARRVENED
                     MTVCYILDQDDDVMDFIFDRDIITVYCPENGTATDEYFCEIIFNSDDTFTLKRLSNYV
                     TIKDRSYPMSKINDVNITGKVVRLFRDFK"
     gene            1002..1148
                     /locus_tag="O0394_002733"
     CDS             1002..1148
                     /locus_tag="O0394_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500747.1"
                     /translation="MNGGENFMVDKNKKQETTRSNPLNKSFEKSGASEKLKSTLSEKA
                     KKKD"
     gene            complement(1145..1759)
                     /locus_tag="O0394_002734"
     CDS             complement(1145..1759)
                     /locus_tag="O0394_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCY0500748.1"
                     /translation="MTQFLGALLLTGVLGYIPYKYLTMIGLVSEKNKVINTPVLLIFS
                     IETCLIWFYSFIIFNNVDLKNLNLIQLLTGLKANILFLFIFVLTVFVFNPLIVKFIIW
                     LINITRKFMKLDCISLLDKRDKLFNNNGKPVFIVIKDFENRIIEEGELKTYNSAGSDF
                     DLLEVERQDFKVSDLPSNDELYIKHTLVDLKQQIKLDLYLMNEY"
     gene            1885..>2213
                     /locus_tag="O0394_002735"
     CDS             1885..>2213
                     /locus_tag="O0394_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCY0500749.1"
                     /translation="MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIR
                     NIKRYSS"
BASE COUNT          950 a          322 c          322 g          619 t
ORIGIN      
        1 gagccgttga gagtttttaa atataaggaa atataaacaa aggagagata catatgaaaa
       61 aagtaatcgg actgctacta gtaagtacat tagctttaac agcttgtggt gaaaaagaaa
      121 aaccaaaaaa agaagaaaat aaaaagtcac aaacacaaaa tcacaaagat agcaaaccaa
      181 aaacgcaaca agaaaaaatg aaaaaagttg aagataaaaa tccacctaat aatagcatac
      241 aaaataattc aaacaatcaa aaccaatcac aaaacaatca acttaataat aattcagatc
      301 catctaataa tactcctgca aatataaatg aaaacgattc acaaaatact aatttaaatg
      361 atgagtatgt cgtttcgcct ggctggacta aagatgaaca ggctaaagct tttgaagagt
      421 acaaaaaagg aaaagaagag gaagcaagag ctggtgctag cgcagtacca ggagccaata
      481 ttaactaata aaacaatata agaaagaaga gctaatatgg aaacaaataa aacaatcgat
      541 ttaatgaatt atgtggaatt tccaaaaaga tacacagagg caaaaggcaa attagttgca
      601 caaccaataa ctactataaa tagcgcaaga agagttgaaa atgaagatat gactgtttgc
      661 tacattttag atcaggatga tgatgtaatg gactttatct ttgacagaga tataattact
      721 gtttactgtc ctgaaaacgg aactgcgact gatgaatatt tttgtgaaat tatatttaac
      781 tcagatgaca catttaccct aaagcgatta tctaattacg ttaccattaa agatagaagc
      841 tacccaatgt caaaaataaa tgacgtaaac attacgggca aagtcgtcag attatttaga
      901 gattttaaat aaacttggct ttaattacga ttaaaagtac ctatatagcg tgacgagaaa
      961 aaggattaaa aaaaatcaaa aaacgcctac tagtgtagac gttgaatggt ggtgagaatt
     1021 ttatggtaga taaaaacaaa aaacaagaaa ctactcgtag taacccatta aacaaaagtt
     1081 ttgaaaagtc aggcgccagc gaaaaattaa aaagcacttt atcagaaaaa gctaagaaaa
     1141 aagattagta ttcattcatt aaatataaat ccaatttaat ttgttgttta aggtctacaa
     1201 gcgtatgttt aatatacaat tcatcgtttg acggtaaatc agatactttg aaatcttgtc
     1261 gctcaacttc tagtaaatcg aaatcgctac cagctgaatt ataggtttta agttcaccct
     1321 cttcaatgat tctgttttca aagtctttta taactataaa tactggttta ccgttgttat
     1381 taaacaactt gtctcttttg tctaataagc ttatacaatc caatttcata aactttctgg
     1441 ttatattaat taaccagata ataaatttaa caattaaagg attaaataca aacactgtta
     1501 aaacaaaaat aaatagaaac aaaatatttg cttttagacc tgtaagcaac tgaattaaat
     1561 tcaaattttt taaatcaaca ttattaaaaa ttataaaact ataaaaccat atcaaacatg
     1621 tttcaataga aaaaatcaat aatacaggag tattgataac cttgtttttt tcactaacta
     1681 aacctatcat tgttagatat ttatatggta tgtaacctaa aactcctgta agaagaagcg
     1741 cccctagaaa ttgagtcatc ttatcaccta ctttttattt tattataaca catttagtac
     1801 ctagtactaa attacgggta gcccgactac ccttattatt ttttaatatt ttatagaaca
     1861 tacgttcttg caggaggtat aaacatgtgg attgaaaaat ttaaaaacaa aaataacgaa
     1921 actaaataca gatactacga gaagtataaa gatccataca cagataaatg gaagcgcgta
     1981 agtgttgtgt tgaacaagaa tacaaaacaa tctcaaaaag aagcaatgtt tcgtttagaa
     2041 gaaaaaataa aagaaaaact gaacaacaag tcgtcaagcg aattaaaaac tttgactttt
     2101 cacgcgctat tagatgaatg gcttgaatat catataaaaa catcaggttc aaagttgact
     2161 actcttaata atataaaaat aagaattaga aacattaaac gatacagctc tga
//
LOCUS       DAKTLH010000236         2193 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.242, whole genome shotgun sequence.
ACCESSION   DAKTLH010000236 DAKTLH010000000
VERSION     DAKTLH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.242"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            108..341
                     /locus_tag="O0394_002736"
     CDS             108..341
                     /locus_tag="O0394_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500750.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
     gene            963..1148
                     /locus_tag="O0394_002737"
     CDS             963..1148
                     /locus_tag="O0394_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500751.1"
                     /translation="MNKHINKKAAKLLEDYKKVQQRKSKETDRGCLKSVIMLIVLFFL
                     IIFGIVACSTNVNFFFQ"
     gene            complement(1801..2151)
                     /gene="scb"
                     /locus_tag="O0394_002738"
     CDS             complement(1801..2151)
                     /gene="scb"
                     /locus_tag="O0394_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-B"
                     /protein_id="HCY0500752.1"
                     /translation="MKFKKYILTGTLALLLSSTGIATIEGNKADASSLDKYLTESQFH
                     DKRIAEELRTLLNKSNVYALAAGSLNPYYKRTIMMNEYRAKAALKKNDFVSMADAKVA
                     LEKIYKEIDEIINR"
BASE COUNT          759 a          306 c          306 g          822 t
ORIGIN      
        1 aaaagatttc tagaaataca attaatgttc atctagatat tgtacgaagt tgtatgaata
       61 agttaaactt aagttgaatc aatttaaatg ttcaaggagg ttaacaaatg gtttttaatg
      121 attgggaaat taacgttgta tactcaggag agcatgaggt tgtaacaaac gaaaagagtt
      181 tatttgtcat tgacgaattt tacgatgttg caatcgcaat ctattattta gatggaaaat
      241 taaaagtagc tcatgtcaac tatggttctg attttacaat tgacgtagct aataaagttt
      301 ttgaattgaa tattcaaaat ccaaatgttg atgagcatta aatattaaaa agcatttaga
      361 atcataaaga gcatactata tatagccttt tacatgtaaa gcaaatgaaa attagataat
      421 ctaattattt caacattaaa caatatatca ttataaagaa gtttttaaaa tagaagtata
      481 aggtggtgaa caaaaattaa cattgtgtat aacattatag tatatatagc atgcatgatt
      541 aatttcggat taattatagt cccattatta acaacaaatg atgcggattt tgtatggact
      601 tcttcatcat taatatatat gattcttctt acgtcgatta tcctaatagt ttatattaaa
      661 actaaagata gcttacagtt aacgatattt atacttaata tagccttaat tattctatac
      721 tgtgcaccat tactattcat ttatgtattc tagtgcttca ttaatatttt aatgcatcag
      781 tttaagtaaa ttccatttgg aaaataaaaa gcaaatatca tcatcaaatt aacatattta
      841 acgttatact ttcataaaat ttgaaacaat tgaacatcct tactttagct ttatatcttt
      901 atttcaagtc atgttttcaa ctattttaat gaattaaaca tatattataa ggagtcaaca
      961 tcatgaataa acatataaat aaaaaagccg caaaactact tgaagattat aaaaaggttc
     1021 aacaaagaaa atcgaaagaa actgatagag gttgtttgaa aagtgtcatc atgcttatcg
     1081 tcttattttt cttaattatt tttggtattg tggcatgttc tacaaacgta aactttttct
     1141 tccagtaaaa gagtgagata gaaatgataa agaaccacta atgatttatt atgtagtggt
     1201 tctttatcat tagctgtagc taatgtgtac ttaaaaaatg aatacatgag taaagctcat
     1261 gcataagaaa tactaattgc taaagaaaaa gtatttcttt atcgatgtcc taccccaact
     1321 tgcattgtct gtagaaattg gggttccaat ttctctatgt tggggccccg ccaacttgca
     1381 catttttgta agctgacttt tcgccagctt ctatattggg gcccccgcca acttgcattg
     1441 tttgttggaa ttggtgatcc tatttctctc tgttgtggcc ccacgagtat tgaaaaaact
     1501 tgttataagc ttattttcat ttagtcaact actaccaata tagcactaga actggaaatg
     1561 ataatttaat attgtacact tttgattgtt taaacatgta gatattttaa aaaataggag
     1621 agcaaagtaa ataattgata tagttatttt gagagtaatc ctaggaacta ttgtatttat
     1681 atttgtctcc cctactttta aatgtcattc attatacata agcattttga tatagaattt
     1741 atcacatatg caaattgaaa acaggttaag accatttttt gtctcaacct gttttattta
     1801 ttatctattt ataatttcat caatttcttt gtatattttt tctaatgcaa ctttagcatc
     1861 agccattgat acgaaatcat ttttcttaag tgccgcttta gctctatatt cattcatcat
     1921 aatcgtacgt ttataatatg gatttaagct tcctgcagct aatgcatata cattcgattt
     1981 gttaagtaaa gttcttaatt cttctgctat gcgtttatca tgaaactgac tttcagttaa
     2041 atatttgtcc agactacttg catctgcttt attcccttct atagttgcta tcccagttga
     2101 tgataaaagt aatgctaatg ttcctgttaa tatatatttt ttaaatttca ttataagctc
     2161 tccttaaaat tgatacttac tgagtatata gtc
//
LOCUS       DAKTLH010000237         2193 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.243, whole genome shotgun sequence.
ACCESSION   DAKTLH010000237 DAKTLH010000000
VERSION     DAKTLH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.243"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            213..2153
                     /gene="ltaS"
                     /locus_tag="O0394_002739"
     CDS             213..2153
                     /gene="ltaS"
                     /locus_tag="O0394_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HCY0500753.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
BASE COUNT          820 a          324 c          347 g          702 t
ORIGIN      
        1 aagcaaatat gagttgtagc ccaaaatact tgttaaatca aagttgaaag ctacaaataa
       61 cgaaaattat aaacttgaat ctgaaagtaa ttactataat tatgacaatg ttaactttta
      121 aacgcactta ttaattaact acataatgtt aatatctaat ttattcaagt actttcgcaa
      181 gatttattat ctaaataacg ggggaaagaa tcatgagttc acaaaaaaag aaaattagtc
      241 tttttgcgtt cttcttatta accgtaataa cgattacctt gaagacgtat ttttcttatt
      301 atgttgattt ttctttaggt gttaaaggtt tagtacaaaa cttaatatta ttgatgaatc
      361 cttatagttt agtagcactg gttttaagtg tgttcctatt ctttaaaggc aaaaaagcat
      421 tttggttcat gttcataggc ggcttcttat tgacgttcct attatatgcc aatgttgtgt
      481 actttagatt cttctctgat tttttaacgt ttagtacttt aaaccaagta ggtaacgtag
      541 aatctatggg tggtgcggtt agtgcatcat tcaaatggta tgactttgtt tatttcattg
      601 atacgttagt ttacttattc attttaatat ttaaaacaaa atggttagac acaaaagcat
      661 ttagtaagaa atttgttcct gtcgtaatgg cggcttcagt agcattattc ttcttaaact
      721 tagcttttgc tgaaactgac agaccagaat tattaacacg tacatttgac cataaatatt
      781 tagtgaaata tttaggacct tataacttta cagtatacga tggtgttaaa actatcgaaa
      841 ataatcaaca aaaagcgcta gcatctgaag atgacttaac aaaagtatta aattatacga
      901 aacaacgtca aacagagcct aacccagaat attatggggt ggcaaagaag aaaaatatta
      961 ttaagattca tttagaaagt ttccaaacct tcttaattaa taaaaaggtt aatggtaaag
     1021 aagtaacacc gtttttaaac aaattatcaa gtgggaaaga gcaattcaca tacttcccta
     1081 actttttcca tcaaacaggt caaggtaaaa catctgactc tgaatttaca atggataaca
     1141 gtttatacgg tttaccgcaa ggttctgcct tttcattaaa aggagataat acgtatcagt
     1201 cattaccagc aattttagat caaaagcaag gctacaaatc tgatgtcatg cacggtgact
     1261 ataaaacatt ctggaacaga gaccaagtat ataaacactt tggtatcgat aaattctatg
     1321 atgcaacata ctatgacatg tcagataaaa acgttgtaaa cttaggcttg aaagacaaaa
     1381 ttttctttaa agattctgct aattatcaag ctaagatgaa atcaccattc tattctcatt
     1441 taattacatt gactaaccac tatccattca cattagatga aaaggatgca actattgaga
     1501 aatcaaacac aggtgatgca acagttgatg gttatattca aacagcacgt tatttagacg
     1561 aagcattaga agaatatatt aatgacttga agaaaaaagg attatatgac aattcagtga
     1621 ttatgattta tggtgaccac tatggtatct ctgaaaacca taacaatgcc atggaaaaac
     1681 tattaggtga aaaaatcaca ccggctaaat ttacagattt aaacagaact ggtttctgga
     1741 ttaaaatccc tggtaaatct ggtggtatca ataatgaata tgctggtcaa gtcgatgtaa
     1801 tgccaacaat tttacatttg gctggtatag atacgaagaa ctatttaatg ttcggtactg
     1861 atttattctc taaaggtcat aatcaagtag ttccattcag aaatggtgac tttataacaa
     1921 aagattataa atatgttaat ggtaagattt attctaataa aaataatgaa ctcataacta
     1981 ctcaaccagc tgatttcgaa aagaataaaa agcaagttga aaaggatctc gaaatgagtg
     2041 acaacgtgct taatggtgat ttgtttagat tctacaaaaa tccagacttc aaaaaggtaa
     2101 atccttcgaa gtataaatat gaaacaggac ctaaagcaaa ctctaaaaaa taatatctaa
     2161 acacgaactc ggattgataa aatatcaatc cgg
//
LOCUS       DAKTLH010000238         2182 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.244, whole genome shotgun sequence.
ACCESSION   DAKTLH010000238 DAKTLH010000000
VERSION     DAKTLH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.244"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(148..954)
                     /gene="yjbH"
                     /locus_tag="O0394_002740"
     CDS             complement(148..954)
                     /gene="yjbH"
                     /locus_tag="O0394_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HCY0500754.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(977..1342)
                     /locus_tag="O0394_002741"
     CDS             complement(977..1342)
                     /locus_tag="O0394_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HCY0500755.1"
                     /translation="MTTTPYDIIGKEALYDMIHYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHIDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            complement(1446..2039)
                     /locus_tag="O0394_002742"
     CDS             complement(1446..2039)
                     /locus_tag="O0394_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCY0500756.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
BASE COUNT          667 a          321 c          327 g          867 t
ORIGIN      
        1 taattttcga actggttaaa ttcgaaaaat atcactaata tatttagatc gatgtgtaac
       61 attacgttct aataatttaa tgttgcaaaa ttaagagtca gtaaaaagac ctcgttacat
      121 ttatggtgta cgaggtcttt aatttattta ttttgatttg attttaggca ttttagattt
      181 ccagaaatcg ccatctggat atttaagttt ttcaattttt tgttgaatgg ctaacttctt
      241 taactctttg tttaaaagtt tttctggcca ttcataaata gtaagtaatt cttccatcgt
      301 tacaagttgt tgttgctgta tataagtttc taatttagga ggaagattct tttcgatagg
      361 tttacccatc aattcattaa ttatataagt atagatgtga tatgggtata atccttcgac
      421 ttttaaacct tcttcatgaa catcttcact aaagaaaacg agagaagggg cttgttcgat
      481 ttccatttct cttgcaatat gcaaatcaat tttcaagctt tcggttagtt tacttttttg
      541 taagtcgtct ttaaatactt ctaaatcaat acctgcattt tgaatacagt cacaaatcat
      601 tgattctgta ataatatctc ttttaggtat gatttcattt tgcattaaat gtataaatcg
      661 ttcggcacgt acacgacctt gtaactcagc tgctttataa gctagggcga tgttatcaaa
      721 gttggatgta ctttgagctt ggcatttcgt taatactttt aacgaaggat ttaatatatg
      781 tctgatacgt atatattgat tatattcaat tcttaatttg gataagattg ctgataattt
      841 gaagcaatcg gagctaaatg gatcgaaaaa tgaataaatt tcgattttac ttacaggtga
      901 tagattgata tcttcacgac tcttattttc cattattcgt aattctccag ccatgtttat
      961 tcacctacaa ttaattttag gaattcacca tatgattagc agttaatctt aagcgctcaa
     1021 ataaataatc tccaacacct tgtggaaacg cagcgcgatt aattgctgtc tgcatatttt
     1081 ctaaccatgc atctctttca aattcagtga ttgtaaaatc tatatgtctt tttcttagca
     1141 taggatgtcc gtgttcttcg gtataaatgt ttggaccgcc caaaaactgt gttaaaaatt
     1201 gtttttgttt acgacttgtt tctgcaaaat ctcctggaaa caggtgatta agtcgttcat
     1261 ctttttctac aagggtgtaa aaataatgaa tcatatcgta taacgcttct ttaccaatga
     1321 tgtcatatgg tgttgttgtc atttaatcac ccattttcaa aaatttactg ttacgaactt
     1381 aagttaatat ataactaata taacatgatt ttaaacattt gaaagaaata tgcatatttg
     1441 ccaatttaat ttatattgtt tgaaagtgtt tcttttttct tgaaaaaacg ttgaacttta
     1501 tttaaaggtt gatgatgttc gaggtttagt tcgtttaata aagattggaa cttttgtaaa
     1561 ccttgattat agtctttaac ttcgaactct aactcataat ccgtagtatc gaaatactca
     1621 cttttatcta aaaccagtaa atcaccttta tatttagttt cttggcgata tgtcgttaat
     1681 gcaccaagta ttgataaagt tgtatctttt acaccaaact gttcaactat aatttgacga
     1741 atgtcatctg gaagattgtc gtttgaaata atcaagttca tctctggttt aatgtcgacg
     1801 atatagttgt attctaatag accaaccttt gctggtgtct ttaaagtcat ttcatattga
     1861 ttgtctttaa ctcttatgcg tagtgcagag cgatgttcct ttaatttgaa atcgggtgta
     1921 tcaatatagt aattgacttg cttaaaaagc acactgtctt taaaatattt ctcttgcaat
     1981 ttattataga ttgatgcagt tatcatttgt ttaaattcta tttcatgatt tgttgccatg
     2041 atatgtatac acctcgtatc aaattcaatt tatcttaact atattatgaa tgacaaagtt
     2101 gaattttaaa agtaatttcc tttatctatt atcaatgtta atttgaccat taaaaatagt
     2161 gttcgtaagt gttttgtatt at
//
LOCUS       DAKTLH010000239         2174 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.245, whole genome shotgun sequence.
ACCESSION   DAKTLH010000239 DAKTLH010000000
VERSION     DAKTLH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.245"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            248..1657
                     /locus_tag="O0394_002743"
     CDS             248..1657
                     /locus_tag="O0394_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0500757.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIRTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSILKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSAGTAVGAGLATFVLGSNPVGWV
                     ILAGLAMSTVFALGTDLIYQNNIFGLKDKVDWVGHKIDNSIDVVKKTTEKSMDSVGNA
                     VSEAKNIISNHINPMKWAW"
     gene            1661..>2174
                     /locus_tag="O0394_002744"
     CDS             1661..>2174
                     /locus_tag="O0394_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0500758.1"
                     /translation="MLFNIKVINKNPRYKVVQYNDEYLLIDLVSTWLVYFFPFINWFI
                     PKRYAKLSEKELENLNVDKQNKNNIFWPVTGSSFLFVVILRKYVHTFEVQLDNKILIS
                     LCFIGFIGIAAFYIYLNKKLKLKIYDDNLDNENRVILVPTFKDGSFIVFTYLLLGGCS
                     ILFLIWLMTIK"
BASE COUNT          818 a          259 c          412 g          685 t
ORIGIN      
        1 cgcaataaaa ttaaaatatt gaaagataat ttttattgat taattgcgaa tatacaaaga
       61 caccattata tccatatgct aaaataagaa aaaaggcgac atgttatctc aatttacctg
      121 catctagact cctttacata agaaataatc atcgccgagt tttaaaattt tacaactttg
      181 tagatgtaaa aagcatttga ttttaaatag tttgaaaatc atatattttt gaaaaaggag
      241 catgccgatg agtattgaca tgtatttaga cagatctcga aaccaagctt caagtgtggg
      301 gaatttgagt caaacaatga attcaaatta tgatgcgttg gaaaaagcaa ttactcaatt
      361 tattaatgat gatgcgctta aagggaaagc gtatacgtca gctaagcaat tttttagtac
      421 ggtgttaatt ccattatcaa caagtatgaa aacattgagt gatttaacga agcaagcttg
      481 cgataatttt gtgtcacgtt atacgagtga ggttgatagc atatctttaa aagaatcaga
      541 gcttgaagaa gatatcagat cattaagtca acaaattacg cgatatgaaa atttgaataa
      601 caatttgaaa aagcatgctt ccgataatca gcaagccatt tcatcgaacc aacaaataat
      661 acgaacatta ggtcaacaaa aacatgaatt agaagagaag ctacgcaaat tgcgtgagtt
      721 taatcaaaaa tcaccagaaa tatttaaaga agttgaagaa tttcaaaaaa ttgtccaaca
      781 aggacttacc caagcgcaga atttttggaa cttttcaaca aatcaattta atattccttc
      841 aggtaaagaa cttgattggg ccaaagcaag tcatgaaaaa tatttgaaag ttgctatggg
      901 gaaaattgaa cataaagcag agaaagaaac tttaaataaa gcagactttg ctgttataaa
      961 ggcatatgcc aaagaacatc cagaagacga tatcccgaaa agtatattga aatatataaa
     1021 tgacaataaa gacagtatta aaagagatat aggattagat attacgtcaa cacttttaga
     1081 gcaaggcggt ataaatgcaa gtaaattcgg tgtatttatc aatacagcag gtggagtgaa
     1141 aggcccagca ggtccaaatt catttgtgga agtcaaacgt acatcaggta atgtgtttat
     1201 agaaaatggt agtaaatttg caaaaggcgg aaaataccta ggtaaaggtg ttgctggtgt
     1261 aggatttggt ataggtatgt atgatgacct tgcaaatgat gataaaacat ttggagaggc
     1321 gttgtcgcat aatggtatga cgcttgcagc tggatctgca gggacagcag ttggagctgg
     1381 attagcgact tttgttttag gaagtaatcc agtaggatgg gtgattttag caggtttggc
     1441 tatgagtaca gtatttgcat taggaacaga tttaatttat caaaataata tttttggatt
     1501 aaaagataaa gtagactggg tagggcataa aatcgataat agtatagatg ttgttaaaaa
     1561 aactacagaa aaatccatgg atagtgttgg gaatgctgtt agtgaagcta aaaatattat
     1621 tagcaatcat ataaatccaa tgaaatgggc gtggtgagtt ttgcttttta atattaaggt
     1681 tattaataaa aatccgagat ataaagtcgt tcaatataat gatgagtatt tgttaattga
     1741 tttagtaagc acttggcttg tatacttttt tccttttatt aattggttca ttcccaaaag
     1801 gtatgcaaaa cttagtgaga aggaacttga aaatttaaat gttgataaac aaaataaaaa
     1861 taatattttc tggccagtta ctggtagttc gtttttattt gtagttattt tacggaaata
     1921 tgtacatacg tttgaagttc aattagataa taagatttta atatctttat gttttatagg
     1981 atttataggt attgcagcat tttacattta cttaaataaa aagcttaaac taaagatata
     2041 tgatgataat ctagataatg aaaatagggt tatattagtg cctactttta aagatggaag
     2101 ttttatagtt ttcacatatc tattattagg aggttgttct atattatttt taatatggct
     2161 aatgactata aaac
//
LOCUS       DAKTLH010000240         2118 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.247, whole genome shotgun sequence.
ACCESSION   DAKTLH010000240 DAKTLH010000000
VERSION     DAKTLH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.247"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            72..1193
                     /locus_tag="O0394_002745"
     CDS             72..1193
                     /locus_tag="O0394_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCY0500759.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIIISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFNYNEENNAIEISE
                     KDDMSIIEMFYKHITDKELSDIQSKKIKNILENELRKED"
     gene            1197..>2118
                     /locus_tag="O0394_002746"
     CDS             1197..>2118
                     /locus_tag="O0394_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCY0500760.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEEVKKEKAQIENRYQQIDLLWQEIESFDDD
                     NIKGLLEVATQQIDKLIENIPLLQARSKEILASVNESKETAIKEFEIIEKKTLENNIL
                     KDNINQLNKNKIDFVQLKEQQPEIEGIEAKL"
BASE COUNT          863 a          256 c          374 g          625 t
ORIGIN      
        1 taattttact tgaaaaggtg aaatgattat attaaaataa aagcgaacaa atgttctata
       61 tggaggcagt aatgaaaatt atacatacag cagactggca cttagggaaa atattaaatg
      121 gcaaacagct tttagaagat caagcgtata ttttagatat gttcgtagaa aaaatgaaag
      181 aagaagaacc tgatatcatt gtgatagctg gagatttata tgacacaaca tatccaagta
      241 aagatgcaat catgttatta gaacaagcga ttggaaagtt aaatttagaa ctgcgtatac
      301 caataattat tattagtgga aatcacgatg gtaaagagag gttaaactat ggggcgagtt
      361 ggtttgaaca taatcagtta tttataagaa cagattttac atcgattaat tcaccaatag
      421 agataaatgg ggttaatttt tatacactcc cttatgctac tgtgagcgaa atgaaacact
      481 actttgaaga tgacaccatt gaaacacatc aacagggaat tacgcgctgt attgaaacaa
      541 tagcaccgga aattgatgaa gatgccgtca atattttaat tagtcatctg actgttcaag
      601 gtggaaagac atctgattct gaaagaccat taactattgg aacggttgaa tcagttcaga
      661 aaggtgtttt tgatatattt gattatgtca tgctaggtca cttgcatcat ccatttagta
      721 tagaagacga caaaattaaa tatagtggct ccttattgca gtattcattt tcggaagcgg
      781 gtcaagctaa agggtataga cgtgtaacaa ttaatgatgg cattattaac gatgtattta
      841 ttcctcttaa gccacttaga caattggaaa ttatctcagg cgaatataat gatgttatta
      901 atgaaaaagt tcatgtgaaa aataaagata attatttaca ttttaaactt aaaaatatgt
      961 ctcatattac tgatccaatg atgagtttaa aacaaattta tcctaatact ttagcgctga
     1021 cgaatgaaac ttttaattac aatgaagaaa ataatgctat agaaataagt gaaaaagatg
     1081 acatgtcaat tatcgaaatg ttttataaac atataactga taaagaatta tcggatatcc
     1141 aatctaaaaa gataaaaaat attttagaaa acgaattgag aaaggaggat taatgaatga
     1201 aaccattaca tttaaagttg aataatttcg gccccttttt aaaagaagaa attgattttt
     1261 ctaaaattga taataatgaa ttgtttttaa taagtggtaa gactggatcg ggtaaaacaa
     1321 tgatttttga tgcaatgact tatgccttgt ttggtaaagc atcaactgaa caaagagaag
     1381 aaaatgattt gagaagtcat ttcgctgatg gtaaacagcc gatgtcagta acatttgaat
     1441 ttcaattaaa tcatcgaatt tataaagtgc atagacaagg cccttatatc aaagaaggta
     1501 atacaacaaa aacgaacgct aaatttgatg tatttgagat ggtggatggc aagtatgaaa
     1561 ttagagaaag taaagtaatt tcaggtaccc aattcattat tgaattatta ggagtaaatg
     1621 cagatcaatt ccgacaattg tttattttgc ctcaaggtga attcaaacgc tttttaatat
     1681 caaacagtcg tgaaaagcaa gggatattaa gaacactgtt tgacagtgaa aaatttgaag
     1741 ctatacgaga aatattaaaa gaagaagtaa aaaaagaaaa agctcaaatc gagaatagat
     1801 atcaacaaat tgacctttta tggcaagaaa ttgaatcatt tgatgatgac aatataaaag
     1861 gcttattaga ggttgccact caacagatag acaaattgat tgaaaatata ccacttttac
     1921 aagctaggtc gaaagaaata ctagcatctg taaatgaaag taaagaaact gctattaaag
     1981 aatttgaaat aatagaaaag aaaacattag aaaataatat attaaaagat aatattaatc
     2041 aactcaacaa aaataaaatt gatttcgttc aattgaaaga acaacaacct gaaatagagg
     2101 gaattgaagc taagttaa
//
LOCUS       DAKTLH010000241         2105 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.248, whole genome shotgun sequence.
ACCESSION   DAKTLH010000241 DAKTLH010000000
VERSION     DAKTLH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.248"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            833..1399
                     /locus_tag="O0394_002747"
     CDS             833..1399
                     /locus_tag="O0394_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCY0500761.1"
                     /translation="MNTKFLGKTLVASALVLTTLGTGLHSSYLGLDTNKVVKTAKAEE
                     KMTDGQLWKKVKDSLHDSDIILSNEYETINVTYLLSNGYSSSVSAPGNDDGGHLTQSI
                     DFKGLKQIDLTKENVYDDFNKKLDAKNTWNSLTEKLKGLGLLQNGQKVSIYSSDSSSP
                     VSGKVGEGVTSGGENTLTKRFINKITID"
     gene            complement(1873..>2105)
                     /locus_tag="O0394_002748"
     CDS             complement(1873..>2105)
                     /locus_tag="O0394_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BsaG protein"
                     /protein_id="HCY0500762.1"
                     /translation="VGVLMFIFNVYFGLEVLGDHSWFYLPITYATRYVYMFIEGSIPV
                     TLTLAIYIIITLLLAVLLFKGFNKWSGRTIKD"
BASE COUNT          767 a          315 c          280 g          743 t
ORIGIN      
        1 gaattgactc atttatttaa atatgcttaa aactcaatat cacgcaatta aaattgactt
       61 tttaggatta ttttggtctt aaatattaat tattgaatta aaattgtaat atttattcgg
      121 aaattaggtg attgaaaata tatggcggaa tggtgaacaa ttcatgtatt tataatcttt
      181 cactttacta tgtattgtgt ttaacaacat tattagcgat tagttcccct attatttatg
      241 acaatatgca gtcaatcaat gaaacatgta tgtatgagta tatttggaac tagtaatgta
      301 ttgttaaata ttgaagatga ttgaattatg gaattttttt cgagatatgt tatttcgggg
      361 atcagatttt gcgattttgc cttggataga gtagttgcga tttgtaaata cgtttttcag
      421 gttcgattca atttcgttgt taagataata tggattagtt tcactgacat gttgatgcat
      481 gtcttttttg ttctgataaa gtataaaatg tttacttaac attacttagt atgacacctg
      541 ctaattcaaa cattatttga gacattcttt tcaaattaat tataaatttt tacctataga
      601 ctagtttgat atttatctac atctcaaaat tctcatcaac aatctttcac atccaacatt
      661 tttactttag tttttataat tcaaaacaac aaaactatgt taaaaaatta ttctattttt
      721 tagttaatag atagttaata catttttgat atttagttaa ttgttctttt aaaaaaatta
      781 ttattatatt ttcattgtaa acgtttacaa tataaaaaaa ggagcaataa aaatgaatac
      841 aaaattttta ggtaaaacat tagtagcaag tgctttagta ttaacaacat taggaacagg
      901 cttacattct tcatacttag gattagatac aaataaagtt gttaaaacgg ctaaagcaga
      961 agaaaaaatg acagatggtc aattatggaa aaaagttaaa gattcattac atgattctga
     1021 tattatttta agtaacgagt acgaaacaat caacgttact tatttattaa gcaatggata
     1081 ttctagcagc gtttctgctc ctggaaatga tgatggaggc catctaacac aatctataga
     1141 ctttaaagga ttaaaacaaa ttgatttaac taaggaaaat gtctacgatg actttaacaa
     1201 aaagctagat gctaaaaata catggaattc tctaaccgag aagttaaaag gtcttggttt
     1261 actacaaaat ggccaaaaag tatctattta ttcttcagat agctcttctc cagtctcggg
     1321 taaagtggga gaaggagtaa ctagtggtgg cgaaaatact ctaactaaac gcttcatcaa
     1381 caaaatcaca attgattaaa ccttaaactc aaatttcctt aattcataaa acattaataa
     1441 ctaacccttt cttacaaacc acctagccca taatatatta aaacgtcatt tttatttcaa
     1501 ttagcggaaa ataaaaaaac cattccgcat taactttggc aagttggaat ggttaaaaaa
     1561 atattttaga agccagcgtt tggcttgctt agacgacatg ttcgcgcatg tcgtcttatt
     1621 tttacattta tataataaca cgcaattcaa taaaatgtct aatcaataaa cctcaattac
     1681 attcaaattc aaaaactttt cacctaatat tcatcgctta aataaacatt acgtttaatt
     1741 aaacagacat tgtcaatcgc gtctaatacc cctgctgtcc gaaaacaaaa ttattttgta
     1801 ttcatattta actgttctct ttcctccctt ttcaacgcaa ctaaatttct tttaccgtta
     1861 gagccccaat ctttaatctt taattgttct accgctccat ttattaaatc ctttaaagag
     1921 taaaactgct aatagcaacg tgataataat atagattgcc aatgttaatg taactggtat
     1981 actcccttcg ataaacatat aaacgtaacg tgtagcatat gtgattggta aatagaacca
     2041 cgaatgatct ccaagcactt ctaatccaaa ataaacgtta aaaataaaca ttaaaactcc
     2101 gacaa
//
LOCUS       DAKTLH010000242         2046 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.249, whole genome shotgun sequence.
ACCESSION   DAKTLH010000242 DAKTLH010000000
VERSION     DAKTLH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.249"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..236)
                     /locus_tag="O0394_002749"
     CDS             complement(<1..236)
                     /locus_tag="O0394_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCY0500763.1"
                     /translation="MNKVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFI
                     ASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGG"
     gene            397..1308
                     /locus_tag="O0394_002750"
     CDS             397..1308
                     /locus_tag="O0394_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0500764.1"
                     /translation="MKRLIGILLCNLFILTACSASVDKTSNSTKTTDYKIENGETLKV
                     PEKPKRVAVLTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVERVAK
                     AKPDLIVVDAMDKNIKKYQKIAPTIPYTYNKYNHKEILKEIGKLTNNEDKAKKWIEEW
                     DDKTRKDKKEIQSKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKD
                     KLQEDKKGYASISKENISKYAGDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYK
                     AEDYWFTDPITLEHLRSKLKKEILNKK"
     gene            1370..>2046
                     /locus_tag="O0394_002751"
     CDS             1370..>2046
                     /locus_tag="O0394_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0500765.1"
                     /translation="MKQLVGIPESMLIPLIARAKEYENEKPIIKDALSKKIFDGLDDM
                     YKNVTCDDMSQIGISIRTVIIDCVTKRLIKDNKDLIVVNIGCGLDTRFQRFNKEKISW
                     IDLDVPESIEIRKTFFKESNSYKMISKSMLDYSWIDDVKNYKFFNSKSNILFIIEGVL
                     MYFDESVMTQLLDTIIKKMGDHNLTFAIEFCSKTIANNTKRHQSVSKLSSQPVFKYGY
                     NDLKKLN"
BASE COUNT          855 a          219 c          337 g          635 t
ORIGIN      
        1 ccacctattt gaattaataa tagtattact atctcaccaa gtatattaaa ctgtgatcct
       61 atatcaactg gggacaagcc agtaactgta aatgcacttg aagctataaa tagggcatct
      121 aaaaaagata ttggcttttt accagtaaaa ggtaaataca ataaaagtgc acctatgata
      181 gttgtagaga agaaaagcat taaataaaaa tataaaggtt tgtggacttt gttcatttta
      241 atttcatact cctttatatt ataaattaat aattagataa tatcataaat gaaaatagaa
      301 atgttactga tgtgtattac taacaggcgt tgactaattc ttttaatatt ttataattga
      361 aaatgaatat caattgaaaa acaaaaggat gatattatga aaagattaat tggaatttta
      421 ttatgtaatt tatttatttt gactgcgtgt tcagcatctg tcgataaaac aagtaactcg
      481 acaaagacta cagactataa aattgaaaat ggtgaaacac tgaaagtacc agagaaacct
      541 aaaagagttg ctgtattaac tggattttat gttggtgatt ttataaagtt aggaatcaaa
      601 ccaattgctg tttcagatat aactaaagat tcttcaattt taaaacctta tttaaaaggg
      661 gttgattata ttggagaaaa tgatgttgaa agagttgcta aagcaaaacc agatttaatt
      721 gttgtagatg ctatggataa aaatataaaa aaataccaaa aaatagcgcc aacaatccca
      781 tatacataca ataaatacaa tcataaagaa atattaaaag aaataggcaa gttgactaat
      841 aatgaagata aagctaaaaa atggattgaa gagtgggacg ataaaactag aaaagataaa
      901 aaagaaattc aaagtaaaat tggtcaagca acagcatctg tgtttgaacc agatgaaaag
      961 caaatataca tatataactc tacatggggt cgtggtttgg atattgttca tgatgcattc
     1021 ggtatgccaa tgacaaagca atataaagat aaattacaag aagataaaaa aggctatgct
     1081 tccatttcaa aagaaaatat tagtaaatat gctggtgatt atatattttt aagtaaacct
     1141 tcgtacggaa aatttgattt tgaaaaaaca catacatggc agaatattga agctgtaaaa
     1201 aaaggacatg taatttcata taaagcagaa gattattggt tcacagatcc tattacatta
     1261 gaacatttga gaagtaaatt aaaaaaagaa attttaaata aaaaataata gaaataagtt
     1321 gtaaaaattt tcttatgcat tggtactaat gtttttaagg agtgattaaa tgaagcaact
     1381 ggttggaatt cccgaatcaa tgttaattcc tttgatagct cgagcaaaag agtacgaaaa
     1441 cgaaaaacca ataataaaag acgcactatc aaaaaaaata tttgatggtt tagatgatat
     1501 gtacaaaaat gttacatgtg atgacatgtc tcaaattgga attagtatac gtactgtgat
     1561 aatagattgt gttactaaga ggcttatcaa ggataataaa gatttaatcg tggtcaatat
     1621 aggttgtggc ttagatacaa ggtttcaaag atttaataaa gaaaaaatat catggataga
     1681 tttagatgta ccagaatcaa tagaaatacg aaaaacattt tttaaagaaa gtaatagtta
     1741 taagatgata tctaaatcta tgctagatta cagttggatt gatgatgtca aaaattataa
     1801 attttttaat agtaagtcaa atatattgtt tattattgaa ggtgtattga tgtattttga
     1861 tgagagtgta atgactcaat tattggacac tattatcaaa aagatgggag atcataattt
     1921 gacatttgcg attgaatttt gctcaaaaac aattgcgaat aatacaaaga gacatcaatc
     1981 ggtatcaaag ttatcctcac aacctgtttt taaatatggt tacaatgatt taaaaaaatt
     2041 gaatga
//
LOCUS       DAKTLH010000243         2035 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.250, whole genome shotgun sequence.
ACCESSION   DAKTLH010000243 DAKTLH010000000
VERSION     DAKTLH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.250"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..256
                     /locus_tag="O0394_002752"
     CDS             <1..256
                     /locus_tag="O0394_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0500766.1"
                     /translation="FVGYLKGKSSLMIFDRHANLKYRYGNRKFWCKGFYVDTVGRNKK
                     VIENYIRNQLQEDIVADQISMEEYLDPFTGEKNKKRKKKE"
     gene            complement(392..1984)
                     /gene="pckA"
                     /locus_tag="O0394_002753"
     CDS             complement(392..1984)
                     /gene="pckA"
                     /locus_tag="O0394_002753"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCY0500767.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
BASE COUNT          624 a          345 c          363 g          703 t
ORIGIN      
        1 atttgttggc tatttaaaag gaaaaagtag tttaatgata tttgatagac atgctaactt
       61 aaagtataga tatggaaata gaaagttttg gtgtaaagga ttttatgtgg atacagtagg
      121 tagaaataaa aaagtgattg aaaattatat tcgtaatcaa ttacaagagg atatcgttgc
      181 agaccaaatc tcaatggaag aatatttaga tcctttcact ggagagaaaa ataaaaaaag
      241 aaagaaaaaa gagtaaccct ttagggttgc tggaatagta gtgcagttgg ctgacttgtc
      301 agtgcccttt tagggctggc cagtaaggaa ggcttatagc cgcagaacaa accacccgtt
      361 cacacgggtg gttttgattt agtattcaaa tttatttgtt gaagctacct ttttcagcaa
      421 tatgttcaac tttttcacca aatttttcga agttcttttc aaaacgttga attaaatctt
      481 ctgcttgtgc tttatatttc tctttgtcgc tccaagcatt aattggattt aaaattgttt
      541 tcggtacatc ttcaatttct acaggaatgc ttaaaccaaa cgtactatct tttgtatatt
      601 ctgcattttt caatttgcca gaaatcgctt ggtttaccat ttgacgtgtg taatgtaagc
      661 tgattctacg tcctacacca tattttccgc cagtccatcc agtattaaca agataaacat
      721 caacatcatg taaatcgata agttcaccta atagatcagc gtaaacagta gggtgtaacg
      781 ggaagaacgg tgctccgaaa catgttgaga atgatggttc aggttctgtc acaccacgct
      841 ctgtaccagc taatttagaa gtgaaaccac tcaagaaatg atacattgct tggtctttat
      901 ttaactttga aatcggtgga ataacaccaa atgcatccgc agttaagaaa ataattgtat
      961 ttggatgtgc tgctttagat ggtactacaa tattgtcaat gtgattaatt ggataagcgg
     1021 cacgcgtgtt ttctgtataa cgattgtctt caaagtccac tgaaccatct tctgcaacta
     1081 cagtgttctc taaaattgca ccatatttga ttgcgtcaaa aatctgtggt tctttttctt
     1141 tggaaagatt aattgctttt gcatagcagc caccttcgat attaaagacc ccgtttttat
     1201 tccagccgtg ttcatcatca ccgattagtt tacggtgtgg gtcagccgat aaggttgttt
     1261 taccagtgcc agatagacca aagaataatg caacatcgcc tttttcacca acgtttgctg
     1321 agcaatgcat gctcataata tcttgcatcg gtaagagata attcattaca gagaagatac
     1381 cttttttcat ttcaccagcg tattcagtac cgccgattaa aatgactttg tgtttaaatg
     1441 aaataatgac aaaggtttca gatttagtac catcaacttc tggatctgct ttaaaatgtg
     1501 gtgcagaaac gatagtgaag ttaggtttaa tctttgtagc ttcttctttt gattcaggtc
     1561 taataaacat atttttagca aataaattat gccatgctaa ttcattgatg actgtaagtt
     1621 ttaacattgt atctttatcg ctaccagcgt agcctttaaa tacgtatagt tcatcttttt
     1681 tatctaaata gtctagtact ttatggtata acttcaagaa agtttcttca tcgataggtt
     1741 gattaatttc tccccaatca atgttatctc tatatgaagg ttcagagaca aaaaatttgt
     1801 ctttaggcga acgaccagta tattttccag tacttgcatt aacagcacca agttctgtta
     1861 ataccccttc gttattgtct aagattttat tataaagttg tgtcgtcgaa agttgaaaat
     1921 gtgacgttgg ttttttcagt aatttgtcaa ttttagttgt ttcagtgtat gtgtctactg
     1981 acatcctaaa tccctccaaa gcgataattt aaatgtaagc ctttacattt aatta
//
LOCUS       DAKTLH010000244         2009 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.251, whole genome shotgun sequence.
ACCESSION   DAKTLH010000244 DAKTLH010000000
VERSION     DAKTLH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.251"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..1153
                     /locus_tag="O0394_002754"
     CDS             <1..1153
                     /locus_tag="O0394_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500768.1"
                     /translation="DGRIPINVWTGDSIARSGRGTLIKLNLENLDALSKLITGETSGM
                     LAECVIFLNESFNISENENKNFADRKKQLSEGFKDKINLFQLEEMERTLISKINSLEE
                     VADETIESISAVKHLLPDFALDALKERINELFKGIKSFIEKVYDSIDNEILEIFKNID
                     HDFRDGVSEEMMKHLKVVKQNIDQIKNQNDIYGRQIADIRSIMKQQDATILDGNFQIN
                     CSGENMVQGLVIPSNYLGRKMKILKDHIDDGIKKIADYVQGIYDEYASKIVDVIKYLI
                     NTIPKIRKNLRHAIEMLNVKKKEFLSLIPNVTCNYIKTKLEELDNTLGKWEPFLNDLK
                     AVSPILDNHLDDIVKNMKPLIVQMIFEPSHYDDMFISRKALTPVFSSVL"
     gene            complement(1196..>2009)
                     /locus_tag="O0394_002755"
     CDS             complement(1196..>2009)
                     /locus_tag="O0394_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCY0500769.1"
                     /translation="IYSQNQVTRQLGDMKGAIKYAVKFYNYPNEYLLTNVKIPKRRKT
                     IEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIASILEVQALTGMRIGEL
                     QALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKRSISINSRTVEIFKKI
                     ILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTKALNMNKKVTTHTFRH
                     THITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDRELTQKLENIPS"
BASE COUNT          701 a          246 c          336 g          726 t
ORIGIN      
        1 ggatgggcga attccaataa atgtttggac aggagattcg attgcacgtt ctggaagagg
       61 aactttaatt aaacttaatt tagaaaatct tgatgcgttg agtaaactga ttactggtga
      121 aacaagtggt atgttagcag aatgcgtaat ctttttaaat gaaagtttta acatctcaga
      181 aaatgaaaat aaaaattttg cagatagaaa gaaacaatta tcagaaggat ttaaggataa
      241 gattaactta tttcagttag aagaaatgga aagaacttta attagtaaaa taaactcact
      301 tgaagaagtt gcagatgaaa caatagaaag tattagtgct gttaaacact tattacctga
      361 ttttgcattg gatgcattaa aagaaagaat taatgagttg tttaaaggta taaaatcttt
      421 tatagaaaaa gtgtatgata gtatagataa tgaaatttta gaaattttca aaaatataga
      481 tcacgacttc agagatggag tatctgaaga aatgatgaaa catttgaaag tagtgaaaca
      541 gaatatagac caaataaaaa atcaaaatga tatttatggt aggcaaattg cagatattag
      601 aagtattatg aaacaacaag atgcaacaat tttagatgga aattttcaaa ttaattgtag
      661 cggcgaaaat atggtacagg gtctagttat accttctaat tatttaggaa gaaaaatgaa
      721 aatattaaaa gaccatatcg atgatggtat taaaaaaata gcagactatg ttcaaggtat
      781 atatgatgaa tatgcatcga aaattgtcga tgtaataaaa tatttgatta atacaattcc
      841 caaaatacgt aagaatttaa gacatgcaat tgaaatgtta aatgtaaaaa agaaagaatt
      901 tttgtccctg attcctaatg taacttgtaa ttatattaaa actaaattag aagaattaga
      961 taatacttta ggcaaatggg agccttttct taatgattta aaagcagtgt caccaatttt
     1021 agataaccat ttagatgata ttgttaagaa catgaagcct ttgattgtac aaatgatatt
     1081 tgaaccatca cattatgatg atatgtttat ttcaagaaaa gctttaacgc cagtgttctc
     1141 aagcgtttta taaagcttgt aaaaaatata agggcaaaaa aagggcggat ttaagctaac
     1201 ttggaatgtt ttcgagtttt tgagttagtt ctctatccat tttttcagtt acatgagtat
     1261 atatgcgaat ggttgttttt tcatctacat gtcctaccct tttcataatt gcttttaaag
     1321 aaacattcat ttctactaat aaagttatgt gtgtatgtct aaatgtgtgc gtggtaactt
     1381 tcttattcat atttaaagct tttgtagttt tcttaagcac accggcgatt tgattattac
     1441 ataaaggatt cccttttttt gttgtgaata tgaaccctct gtcaacatag ctcgaattcc
     1501 atcttttcaa cattttgttt tccagtatta tctttttaaa aatttctacg gttctagaat
     1561 tgatgctgat acttcttttt gaacttatag tctttgtagt gtctttgtat ccgaatcctt
     1621 cctcgtattt aatgcggtga attgtacctg ttatattgat agttttgttt aataaatcta
     1681 tatctttttc ctgcagtgct tgtagttctc ctatgcgcat accagttaaa gcctgtactt
     1741 ctaagatgct ggcaattaaa atgcgatttc gcttgtgtaa cttattatca tttagtatat
     1801 gatcacgtat ctgtaggact tggttcattt ctaaatagtt gtacatttta gattcatctt
     1861 tttcgatatc ctctattgtt tttcttcttt taggaatttt gacattagtt aacaaatatt
     1921 catttggata attgtaaaat ttaactgcat atttaatagc tcctttcata tctccgagtt
     1981 gacgggttac ttgattttga gaatagata
//
LOCUS       DAKTLH010000245         2001 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.252, whole genome shotgun sequence.
ACCESSION   DAKTLH010000245 DAKTLH010000000
VERSION     DAKTLH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.252"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..118
                     /locus_tag="O0394_002756"
     CDS             <1..118
                     /locus_tag="O0394_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0500770.1"
                     /translation="YKNRWGLFGYCRFIPYLKKRLNNKIVLMKYKVPKRQRH"
     gene            complement(490..1713)
                     /locus_tag="O0394_002757"
     CDS             complement(490..1713)
                     /locus_tag="O0394_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCY0500771.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLTDLFDKYDIKS
                     EILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWSYPPFQLTEKDGKLYGRG
                     TTDMKGGLMALVVSLIELKEQNELPHGTIRLLATAGEEKEQEGAKLLADKGYLDDVDS
                     LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNLFKEKYSE
                     LKQQDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIQYLKEK"
BASE COUNT          652 a          326 c          293 g          730 t
ORIGIN      
        1 ttataagaat cgatggggct tatttggata ttgtagattt ataccttatt taaaaaaaag
       61 gttaaacaat aaaatagtac ttatgaaata taaagttcca aagagacaga gacattaagt
      121 tcttagacaa tgtaaaaaag ctgatttcta taaattattt gatagaaatc agcttttttc
      181 aaatgtattt gataatttac agctcgttga gctgctgttt tctagggatt tatgtcccat
      241 tccttttacg agaaggtttt tacgagtttt atgtaactat tagaagctat ctttttatat
      301 gaccattagt caatatatgt atgacaaatc agcacaataa tttcagtttt acatcactta
      361 atcaatgaaa atgtgcttgt tgatatttga atatttacat tcaaacaata ttcatcgcta
      421 tttctatcaa cttaaaattc gcaataaaaa actgccagca cgcgaatagg gcagctgaca
      481 gcatcgttct tatttttctt ttaaatattg aatggaagcc tctttaaaaa tatcaatata
      541 tttcagatac atatcttttt caatatattc atcgatttga tgtgccatta atggattacc
      601 tggtccaaaa atggctaaat caacattgtc cttattatct cctaagaaac tagaggcatc
      661 tgttgcacct acaagcgctg aaacaaatat ttcgtcttgt tctacataac tagaagctac
      721 atctttaatc gtagtaatta atttgctatt tttatcgctt gttacaggtc ggtgattgct
      781 tggaatatcg agtgaaagct tattgctatc cacatcatta atgatatttt ggaaaaacga
      841 ttctataaag tcgttatcat actcaggaac tggtcttacg ttaaattcaa gtgaagcttc
      901 atctggtaca gagttaaatt gtttgccgcc atttataatc gaacatacag ctgtaagacc
      961 agatgcataa tttgcatcct cttcagaaat ttcttttcca atcaatgatt tgaacatagg
     1021 cgcaacatct aattcatgtt tagtatcttg ttgtttaagc tctgaatatt tttctttaaa
     1081 tagattataa aattcaagca gtgtatcaat tgcattgtca ccaataaatg gaactgagct
     1141 atggacagct ttaccagttg cagttacttt acatgacata gaccccttat gtgcataata
     1201 aattccagat ccagttggtt cagcaataat taagctatcg acatcgtcta aatagccttt
     1261 atcagctaat aatttggcac cttcttgttc tttctcttcg ccagcagtag ccagtaatct
     1321 aatcgttcca tgaggcaatt cattttgttc ttttaattcg attagagata cgaccaaagc
     1381 cattaaaccg cctttcatat ctgtagtgcc tcggccatat aatttgccat ctttttctgt
     1441 cagttgaaaa gggggatatg accaattatc ttgatttcct gcatcaacaa catccatatg
     1501 accactcaat gcgagtatag gtgagccgtt accgatttct gcaacgatat tggcgcggtg
     1561 ttcattaact ttcaaaattt cagatttaat atcgtacttg tcgaataaat ctgttaaata
     1621 attacaaacg tctatttcat tattattttc agtttgtagt tcaacaatat ctgctagtaa
     1681 ttgaattttt tctttttcac taaaagttgt cattaagctc acacctttca aagtagtata
     1741 tatatttata taaacataat ttttattctt aaacataatt taaaatgcag aaagtagtgt
     1801 gttatttgat ttcgttctat gaaattaact gtctagtatc aacgatctta ttaacgctga
     1861 tatcggatat gcttaatttt atagtaaatt gtatgagaaa gatgcaggat attatttagc
     1921 taataattat atataaattt caaatcgatg gttattaact actaaataaa aatatttgct
     1981 aaattaatga ctttgtacac a
//
LOCUS       DAKTLH010000246         1990 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.253, whole genome shotgun sequence.
ACCESSION   DAKTLH010000246 DAKTLH010000000
VERSION     DAKTLH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.253"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            130..306
                     /locus_tag="O0394_002758"
                     /pseudo
     CDS             130..306
                     /locus_tag="O0394_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(284..385)
                     /locus_tag="O0394_002759"
     CDS             complement(284..385)
                     /locus_tag="O0394_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500772.1"
                     /translation="MQGFKEKHQELKKALCQIGLMRSISEVKQLNIA"
     gene            complement(508..603)
                     /gene="pepA1"
                     /locus_tag="O0394_002760"
     CDS             complement(508..603)
                     /gene="pepA1"
                     /locus_tag="O0394_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HCY0500773.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
     gene            806..949
                     /locus_tag="O0394_002761"
     CDS             806..949
                     /locus_tag="O0394_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0500774.1"
                     /translation="MTRERRSFSSEFKLQMVRLYKNGKPRNEIIREYDFTPSTFVNGG
                     YKM"
     gene            1553..1936
                     /locus_tag="O0394_002762"
     CDS             1553..1936
                     /locus_tag="O0394_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500775.1"
                     /translation="MSNQNLFDELEKKGYKLEDIFTKEEIKKYKAEDQLRAGKTQYVE
                     TGKDTATLYLSSAYTKTIAALGAGAISVISALTGGLVGAGVGGFLGSIAASNIDTSKG
                     IYIKLKTKKNAAGEYVLTGEKWGYQ"
BASE COUNT          756 a          275 c          348 g          611 t
ORIGIN      
        1 ttgtagtatc tataaaaatc tcgacactat taaaatgata aagtgccgag gttttcttac
       61 ttatttagtt aattcaaagt ttatgccaga ttcataagaa tttgtgacac ttttaatagt
      121 gtaaccattg attattacaa tttatcaaat ggtcctttag aaggtataaa taacaaaata
      181 aaattaatta aacgtacatc ttttggttat ggaagttaca atcatttgcg caatcgaata
      241 ttattatgtt caaaacttta cgctccaaaa agtaaaaagg aagttaagca atgtttagtt
      301 gcttaacttc ggatattgaa cgcatcagtc caatttgaca tagagccttt tttagttctt
      361 gatgtttctc tttaaaacct tgcatatttt acaaaaagaa aaattagcag tataattaag
      421 acaacgaaaa taagtattta cttatacacc aatcccctca ctatttgcgg tagtgagggg
      481 atttttattg gtgcggctat atgtcaccta ttttgtattg cgtctactta gccaataaga
      541 aaaaaacgca atggcacagc cactgatgac tggtgctatg atgtgaacga aaataagcat
      601 caccttatac acctcctctc tgcgtctaaa ttgacgcctg agagataggc gactctacta
      661 ttatatcatc ggcaaatata caagcacagt cacttgcttc tgataagtta tatgattcta
      721 gctgatagat tgaatcgtct acacttaatt ggacaaattc tatgagaata gatattgtta
      781 atttaagaaa gtaggcgatt ttattatgac aagagaaaga agatcattta gttcagagtt
      841 taagttacaa atggttagat tatataaaaa tggtaagcct aggaatgaaa ttatacgcga
      901 gtatgatttc acaccttcga cgtttgtaaa tggcggttat aaaatgtagg aaaatggata
      961 aagcaacatc aaaacacggg tacattcaat caccaagata acttatcgga tgaagaaaaa
     1021 gagctgatta aattacgcaa agaagttcaa catttaaaaa tggagaacga tattttaaag
     1081 caagtagcgc tgattatggg gcaaaaatag aagtcattca aaagaatgca catcaatatt
     1141 cagtatcagc aatgtgtaaa gtcctgataa tactaagaag tacctattat gattctataa
     1201 aaagaaaaga taataaaatc actaaagatg attcaaacat agaacatgcc gtcataaata
     1261 tttttaattc taatagaaaa gtctttggta caagacgaat taaaaatcat ttaaatgaca
     1321 agggtctcac tgtatctgga caaaagatag gtcgatcatg aaaaaatcta gtttctgttt
     1381 atacgaaatc taaatacaaa aatcatctaa aagaaactaa tgaaaaacga attaaaaatc
     1441 tttattatta gctgctggtg tattatttgt tagtccaatt tcattatctt ataattcaga
     1501 tgtagctcat gctgaagata agttagacca ttctcaagca aaggtaatat atttgagtaa
     1561 ccaaaattta tttgatgaac ttgagaaaaa aggttataaa ctggaagata tatttacaaa
     1621 agaagaaata aaaaaatata aagctgaaga ccaattgaga gcgggtaaaa ctcaatatgt
     1681 agaaacaggt aaagatactg caacattata tctttcttct gcatatacaa aaacaatagc
     1741 tgctttaggt gcgggggcta ttagcgttat ttctgcacta acaggaggtt tagttggcgc
     1801 aggtgttggt ggtttccttg gatctatagc tgcatcgaat attgatacta gtaagggaat
     1861 atatataaaa ttaaaaacta aaaagaatgc agccggggaa tacgttctga caggagaaaa
     1921 atggggatat cagtaagggt gattttatgg atagattaaa atattcactt aaagttggaa
     1981 ttttagcatt
//
LOCUS       DAKTLH010000247         1983 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.254, whole genome shotgun sequence.
ACCESSION   DAKTLH010000247 DAKTLH010000000
VERSION     DAKTLH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.254"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..1561)
                     /locus_tag="O0394_002763"
     CDS             complement(<1..1561)
                     /locus_tag="O0394_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCY0500776.1"
                     /translation="MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKSEFDLLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEN
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITV
                     DLFNEGIDIPEV"
     gene            complement(1563..1955)
                     /locus_tag="O0394_002764"
     CDS             complement(1563..1955)
                     /locus_tag="O0394_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HCY0500777.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEKMSLPLMWEFPGGKVEKNE
                     TEKDALIREIREEMKCDLIVGDKVITTEHEYDFGIVRLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
BASE COUNT          633 a          372 c          218 g          760 t
ORIGIN      
        1 tgacttctgg aatatcaata ccttcgttaa aaagatctac agttataata tagttaatct
       61 taccttcttt taatttttct attacaattt ggcgataatt aacactatca tctcctgtta
      121 aagcaacact tttaatccct tttgaactta gtttatccgc taaatcataa gcttcttttt
      181 tgctacttac aaaaatcaat ccttgtaaaa tttcacctga atatccatag taatctgtct
      241 tttgaataat ataattaact ctttcatcag aagttaaata tctcagttta gttacatcat
      301 cttctttaat accttgatgt acataatcag tcacaccaaa ataatgaaat ggacataaaa
      361 tatcactttc taatgctgct tgtaacctta tttcatatgc aatattataa tcaaacagtt
      421 caaatatact taattcatct gatctttctg gtgtagcagt cattcccagc atgaacttag
      481 gtttgaagta gttaaatact cgttgataag tagatgcagc agaacgatgt gcttcatcaa
      541 aaacaatata atcaaattca ttttcatcaa attgcttaaa attatcatct ctagataatg
      601 tttgaatggt tgcaaataaa tatttggcat cgacatctct atgttttcct gtcaacaatc
      661 caaaatcact atcatttttt ataggtaata cttttttaaa ttcttcctta gctctattta
      721 aaatcccctc attatgaaca ataaataaaa atttattagg gtttacttct ctaacatcta
      781 atgcacataa aatcgtttta cctgtaccag ttgcagatat tattaacgcc ttatctttgg
      841 ctttatccct aatagctttt aatgacctta atgcttctgc ttgcattaaa ttgggtacaa
      901 tttccactga ttttttcacc ttatcagcta gcagcatttg agtttgttca acctccgcta
      961 atttttctaa ggaacggtac tcaaatgatt ctttatatga attaatccat tgctcagtca
     1021 gtggggtact cttttgccat aacaagtcaa attcactttt tacactatca actaaatcgc
     1081 catttttcat agtagacagt aaaacattat gctcataatt aacctttaac gcattagatg
     1141 ttaaattaga gcttcctatt accatagaac tataatcctt atgctcaaaa atatatcctt
     1201 tggcatggaa tccagcaata tcagttaatc ttacctctac attttttaat ttaagtaatt
     1261 ctccatacat tttaggacta ttaaacccta agtaattaga tgttaatatt ttccctttaa
     1321 cacccttatt gcttaaatct aatagttgag cctttaagct ggctaaaccg ctttctgtta
     1381 taaaagccac agaaaaataa aacgtttcac atttttgaag ttcatctata attgttgaaa
     1441 gaactttttc atttttatta tttactaaaa gcttcggtgt ataattccct ttatgagaaa
     1501 tatgtttgtc tataaaccct ttatgtaaag attgattgaa atcatttagt aatctactca
     1561 tattatccct cagtcataat tttattaacg gctggtatat ccgctggggc ccaatttaat
     1621 ttatcaagtt cgtttattga caaccattca atactcttat gttcagttag agttggtaac
     1681 tctttgttta aagtacattt gtatgttgtt aacctaacaa ttccaaaatc atattcatgt
     1741 tctgtagtta taactttgtc tccaacaatt aaatcacatt tcatttcttc tctaatttct
     1801 ctaatcaaag cgtctttttc agtttcattc ttttcaacct taccgccagg aaattcccac
     1861 attaaaggca gactcatttt ttcacttctc tgtgcacaaa gaattttgtt atcagaaaaa
     1921 ataatagctc ctactacatt gattactttt ttcataagac tcacccttca atttaaaatc
     1981 atc
//
LOCUS       DAKTLH010000248         1967 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.255, whole genome shotgun sequence.
ACCESSION   DAKTLH010000248 DAKTLH010000000
VERSION     DAKTLH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.255"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(108..350)
                     /locus_tag="O0394_002765"
     CDS             complement(108..350)
                     /locus_tag="O0394_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HCY0500778.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            451..774
                     /locus_tag="O0394_002766"
     CDS             451..774
                     /locus_tag="O0394_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HCY0500779.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            complement(833..1897)
                     /locus_tag="O0394_002767"
     CDS             complement(833..1897)
                     /locus_tag="O0394_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0500780.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
BASE COUNT          648 a          359 c          315 g          645 t
ORIGIN      
        1 tccaatttga cagcgaaaaa agacaggtaa taactgatta taaataattc agtattcctg
       61 tctttgttgt tattcataat atgttctgtt aacttaatat ctttatatta gaatacttgt
      121 tctacttcta ttacaccagg cacttcttcg tgtaatgcac gctcaatacc agctttaaga
      181 gtgattgtag aacttgggca tgtaccacat gcaccatgta attgtaattt aacaataccg
      241 tcttccacgt caatcaatga gcagtcgcca ccatcacgta ataaaaatgg acgaagacgt
      301 tcaataactt ctgctacttg atcaaacatc gttgtatctt cagtaggcat caggcaagtc
      361 tcctttcgag agatgaattt gataatcttt attagtatat tataatagat agtgtaataa
      421 aaatctatca aaccaatggg ggataaatat atggagcacg tgagtgtggt agtttatggg
      481 gcagatgtta tatgtgcaag ttgcgttaat gcgccaacat cgaaagatat ttatgactgg
      541 ctacagccgc tattaaaaag aaaataccca aatatatcat ttaaatatac gtatatagat
      601 attacaaaag ataatgacaa cttaacagat catgatttac aatttattga aagaatagaa
      661 caagatgaac tattttatcc attaattaca atgaacgatg aatatgtagc agatggttat
      721 atacaaacaa agcaaatcac tcgatttata gaccaaaagc ttgtgaatga ataatataca
      781 cataactaaa agcaatgtca gtaatagtgt gaactgacat tgctttttaa ttttaaccat
      841 tgtgatattt atataaccaa agtacgccgg attttaaaat agaagcgagt cgtcctgtta
      901 cagtgcggtc catgatatat gcaaaacctt gcttatctcc taatgaccca acgatacctt
      961 gtacctttag ttccggcatt ttgtcaggta atggttcatt tagccattgc tttttaagca
     1021 catcggcaat ttgatcacct tgaacttcgg ctaactgagc acttggcgca tgtggtaaat
     1081 cagcacaatc accaactaca tagacgttac gatatgttgg tacttgatga tactggttaa
     1141 ctatcacgcg tccattacta tttatatcaa tcggcaagtt acgaacaact tcaacaggtt
     1201 gaattcctgc tgtccataca actaaatcaa tatctttagg ttcatcacag ttatatattt
     1261 taccaggttc aactttatta atatttgaat ttggaacaac ggtaacatta tttttggcga
     1321 accatttcgc aacatactta cttaattttt ctggaaaatt tcttaaaatt cgcggcccac
     1381 ggtcataaag atatatttcc aagtctgatc tactttctct taattcgctg gcaagttcta
     1441 tgccgcttaa tccagcacca acgataccga ctttagcacc ttctggtagt tcactaatac
     1501 tatggaaagt atcccgagcc tttgagagtg tttgaatact atgtgtatat tcttcggctc
     1561 ctggaacgtt atgatattta tcttcacatc ctaaaccaat gattagctca tcataatcaa
     1621 ttttagaatt accgactgag acaatttgag catctaaatc tatgtcgtta atttcaccat
     1681 aaactgtatt cacttgtgga tgattaggga atttcatacg aacatcttta tctgatttcg
     1741 tgcccgcagc taaagcataa aattctggtt tcaatccatg aaatggcata cgatcaacta
     1801 atgtgactgt ataatcttgt ggtaaagaag tagttaaaat gcgtgacatg atacgcatat
     1861 taccatatcc gccgcctaac aaaactaagt ttttcataat gaagtacccc ttttatatgt
     1921 taatatttac tttaattttg aaaagatcaa agtgcataga tatattt
//
LOCUS       DAKTLH010000249         1963 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.256, whole genome shotgun sequence.
ACCESSION   DAKTLH010000249 DAKTLH010000000
VERSION     DAKTLH010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.256"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(70..762)
                     /locus_tag="O0394_002768"
     CDS             complement(70..762)
                     /locus_tag="O0394_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HCY0500781.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            1147..1944
                     /locus_tag="O0394_002769"
     CDS             1147..1944
                     /locus_tag="O0394_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCY0500782.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
BASE COUNT          681 a          271 c          342 g          669 t
ORIGIN      
        1 cgaagtattg tataaataga gaacagcagc aagatatttt ctaattgaaa atcatcttac
       61 tgctgttttt taggaatcga tggggtttaa aaattgatga tagttatcaa tggatatgtc
      121 tctggtttga atcgtcttac cgaatgaata cgaaatatct ataatatttt caatttcgag
      181 ttcggcagtt ttgtcttgag caatgtcata aatgactgca tgccaatgtc catgtttaca
      241 caaaaggcca ataaatataa cgttttcagc tttaatatta tcatcaaccc tatgatctac
      301 taacatcaca tttcgttctg tacaataaat taaaatgtcg gaaaacagca ttgggagtgt
      361 catatgttgt gtgtcatcaa atttaatata aaagtccatg cgtttaagca attttcttaa
      421 gcgtgtttgt ggaagactta aacattgttt tataattgcg ttgatttcgc ttttatatgg
      481 taatgtcgag tacgactgac tttcattaag cactaaaaat aaggctgata attgtgtttc
      541 agataagttt aatgcaattt tagattgtgc ttgttgtatt tggtaaccac catttttccc
      601 ataatgcgca taaattttaa caccttgatt ttctaaatca tcaatatctc ttaaaattgt
      661 gcgtttggat acgttgcaat atttagctaa ttctaaagcg gtcatttttt tattttgttg
      721 aattgcagta attattaaat tttgcctttc agctttattc atgacagcca cctcctaaaa
      781 gatgatatat tacacaaagt ataacataag agtgacaatg aggacatttt caatatcttt
      841 taaaaaaaat cgtaaaaatt ttgtggaaaa cgcttacatt ttaaaaaaga ttgtgctatg
      901 atattacttg taacaggggg agcgattaaa caaaggggta gagctatata acagatagct
      961 tttaatcgtt caagttacat cacaacatct tcaatattta ttacttactt tcctttctat
     1021 ttgtcggcta gcacgtgact agccgatttt tttatgcata aaatgaagaa gtcttcagtt
     1081 gtattgttga ttcttttaat atagggtaat aataaaatga taatgatatg agaagtggtg
     1141 gacgaaatga cagataaaac attacagcaa atagataagt taatatgttc gtggttgaaa
     1201 caaatagaca atgttattcc gcaattaatt atggaaatga ctacagaaac taagcgtcat
     1261 cgatttgatt tagttacaaa tgtagataaa cagattcaac aacaattcca acaattttta
     1321 gcaacacatt ttccggaaca ccaactatta gcggaagaaa aaagtaatga aatgattacg
     1381 aatgaaatta atcatttatg gattatggat cccattgatg gaactgccaa tttagtaaaa
     1441 cagcaagaag attattgtat tatattggcg tatttttatg aaggtaaacc aatgttatcg
     1501 tacgtatatg attatccgca taaaaagctt tataaagcaa tacgaggaga aggtgctttt
     1561 tgtaatggaa ttaagatgga agaaccacca tcgttaaaat tggaagacgc tattatatcg
     1621 tttaatgcac aagtgatgaa tctagatacg gtgcaagatt tatttgacgc ctcatttagt
     1681 tatcgtttag ttggtgcttg cggattagat tctatgcgag tggcaaaagg tcaatttggg
     1741 gcacatatta atacaaatcc taaaccttgg gacattgctg cacagttttt atttgcagaa
     1801 ctattgaatc taaaaatgac gactttagat ggtaaggcaa ttgatcattt aaagggtgca
     1861 ccttttatta taagtaataa agcatgtcat gaaacggtac ttaaaatttt aaatgcaaat
     1921 ggtggttatc aaaaatacag atagatattc tctagagata aaa
//
LOCUS       DAKTLH010000250         1960 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.257, whole genome shotgun sequence.
ACCESSION   DAKTLH010000250 DAKTLH010000000
VERSION     DAKTLH010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.257"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            173..967
                     /gene="tagH"
                     /locus_tag="O0394_002770"
     CDS             173..967
                     /gene="tagH"
                     /locus_tag="O0394_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HCY0500783.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            complement(1028..1792)
                     /locus_tag="O0394_002771"
     CDS             complement(1028..1792)
                     /locus_tag="O0394_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HCY0500784.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
BASE COUNT          648 a          326 c          305 g          681 t
ORIGIN      
        1 taagtttagt ttttaaatat cattgttatc attgatgatt aacattttgt gtcaaaacac
       61 ccactctgat aataacaaaa tcttctatac actttacaac aggttttaaa atttaacaac
      121 tgttgagtag tatattataa tctagataaa tgtgaataag gaaggtctac aaatgaacgt
      181 ttcggtaaac attaaaaatg taacaaaaga atatcgtatt tatcgtacaa ataaagaacg
      241 tatgaaagat gcgctcattc ccaaacataa aaacaaaaca tttttcgctt tagatgacat
      301 tagtttaaaa gcatatgaag gtgacgtcat agggcttgtt ggcatcaatg gttccggcaa
      361 atcaacgttg agcaatatca ttggcggttc tttgtcgcct actgttggca aagtggatcg
      421 taatggtgaa gtcagcgtta tcgcaattag tgctggcttg agtggacaac ttacagggat
      481 tgaaaatatc gaatttaaaa tgttatgtat gggctttaag cgaaaagaaa ttaaagcgat
      541 gacacctaag attattgaat ttagtgaact tggtgagttt atttatcaac cagttaaaaa
      601 gtattcaagt ggtatgcgtg caaaacttgg tttttcaatt aatatcacag ttaatccaga
      661 tatcttagtc attgacgaag ctttatctgt aggtgaccaa acttttgcac aaaaatgttt
      721 agataaaatt tacgagttta aagagcaaaa caaaaccatc tttttcgtta gtcataactt
      781 aggacaagtg agacaatttt gtactaagat tgcttggatt gaaggcggaa agttaaaaga
      841 ttacggtgaa cttgatgatg tattacctaa atatgaagct ttccttaacg attttaaaaa
      901 gaaatccaaa gccgaacaaa aagaatttag aaacaaactc gatgagtccc gcttcgttat
      961 taaataaacc gaaaaaaccg agaatctcca tttaaggatt tcctcggttt tatttttgtc
     1021 atcatgatta tttcgccttt tttatttttc tttttgcttt ggctatttta tacataaata
     1081 ttggaatact ctttaatcta ccaatacgtt tccaatctat taatgctcta tatatccatt
     1141 caatgtttaa ttttctaaag atataaggcg ctctcttttt agccccagca aatacttcaa
     1201 gagaaccacc tacgcccatc atcactgtag attcaaattg gttttcatgt gtcataatcc
     1261 attcttcttg tttagggaat cccataccta caaatatgta atcaggttta aacagtttaa
     1321 ttcgtttcac tactgtctca tcttctaaat caatataacc gtgatgatgt gcaaacgata
     1381 tgtttggata tctttgttgc aatgcatatt gtgccgcttc tacaacttca ttagttgccc
     1441 ctagcaaaaa tactttttga tgatttacat gagcaatttt caaacattca tccatcaact
     1501 caataccagg tatacgatgc gctagaggtt gctttaaacg atgcgaagct ttgactactc
     1561 ctgtcccatc agcaacaata tagctcgctt gatttattaa ctctaaatac gcttgatgtg
     1621 tcgtcgcgta attcactatt tcagggttgg ctgttactat aaaaagatta ttcgttgatt
     1681 gatttgcaaa aaaggtttta atattttcaa ccatttgcaa cattgttgta ttatcaaaat
     1741 cgacccctaa aatgtcaact ttggctgtat tggatctttc ttcaacagtc ataacaggaa
     1801 tctcctatct tatttcatta ttatgttagt gtaatcaata ttaacagtat ttatattcat
     1861 cctatttata tcgaaatgat tttgctttat tttaacacat attcaccttt tcaaaaatta
     1921 aaattagttt aactaaggta agtctaaagt ctaacgctat
//
LOCUS       DAKTLH010000251         1957 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.258, whole genome shotgun sequence.
ACCESSION   DAKTLH010000251 DAKTLH010000000
VERSION     DAKTLH010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.258"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(213..1130)
                     /locus_tag="O0394_002772"
     CDS             complement(213..1130)
                     /locus_tag="O0394_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCY0500785.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNNDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTSEYIERHAELPQDFEDLLDTIRKRDA"
     gene            complement(1130..1621)
                     /gene="lspA"
                     /locus_tag="O0394_002773"
     CDS             complement(1130..1621)
                     /gene="lspA"
                     /locus_tag="O0394_002773"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCY0500786.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRILTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEK
                     EVK"
BASE COUNT          681 a          348 c          242 g          686 t
ORIGIN      
        1 gacgctcaat taaagactct ttatgttgta cttaaactgt tataaggata atactaatca
       61 ggcgctttgt caatctatat tgaaaaatta accatactca gtaaaaatgt taaccttaat
      121 ctattttata atgtgttttc atttaaaaaa acgaaaacga taaacactta tagtttacat
      181 aataatgtta tcgtaattat agcacgacac aattatgcat ctctttttcg aattgtatct
      241 aataaatctt caaagtcttg tggtaattca gcatgtcttt caatatattc acttgttact
      301 ggatgttcga atccaataag tccagcatgt agagcttgac caccaatatc caatgtcttt
      361 ttcggtccat actttggatc accaactaat gggaagccaa tatatttcat gtgaacacgg
      421 atttgatgcg tacgtcctgt ttcaagttga cattcaacaa gcgtataatc tttaaaatgt
      481 tctagtacgt taaaatgtgt cactgcttcc ttaccatcat caacaacagc catagattga
      541 cgatcatttt tgtttctacc aattggcgca tcgattgtac cgtaatcatg aggaatattc
      601 ccgtgaacta aagcgatata ttttctttta acagatttat ccattaattg ttctacaagc
      661 ccacgatgag caatatcatt tttagcaacc attaataaac cagaagtatc catatctata
      721 cggtgaacaa tacctggacg aatttctcca ttaatacctg acaaattttt aatttgatac
      781 attaaaccat taactaatgt attggtataa tgccctggtg atggatgaac taccatgcct
      841 ttcggtttat atacaactgc aacatcgtca tcttcataat aaatatctaa atttaaattt
      901 tcaggtagaa tatcagcttc aaccacttct ttttcagtga caactatatg atcattaagt
      961 ttcactttat aattagattt aacaacttta tcgtttgcaa cgactaaacc tgctttaatc
     1021 caatcttgta tctggttacg agaccaatca ttatttaatt caggcagcaa cttatctaca
     1081 cgcatacctg tttgttcttt atctgtaatg ttaaattcat aagtctccat tacttaacct
     1141 ccttctcctt tttattggaa gtatccttta ataaggcaat aataattaat attacaccaa
     1201 ttgttaaact tgaatctgcg atattaaata ttggaaaatc ataaccaaaa atatttgtat
     1261 caataaagtc aacaacttct cctgttaaaa ttctatcaat aaagtttcca agtgcacctg
     1321 caaaaagtaa actaatagca acttgcataa acaaattata ttgagcatct ttaataaaga
     1381 aatatactaa ggctattaat ataataatgg taataataaa gaaaaatgtc atttttccac
     1441 tcaatattcc ccatgcagca ccattatttc gatgtgatgt tatgtttaaa aagtgcggta
     1501 tcacttcaaa tgaatctcca attttcattg tagtagctat aatatattta gtaacttggt
     1561 caaatataac gacaaatact gctattaaaa tggaagtgcc aataaaatat tttttgtgca
     1621 ttttcgttcc tccaatcaat cgtccatgag acaactcttt atattatagc ttacacctgc
     1681 taataaaaaa agtaagcata ttacattaaa tctaatgtta ctaactcaat acttgataaa
     1741 ctactatgtt ttgacattaa atatgaactt aattattcat ttatcatatt taagatgaca
     1801 ttaaaaatta ggaaagcagg ctggaacata aatccctaaa aagacagtag taagatattt
     1861 tctaattaaa aattatctta ctgctgttct ctatttatac aatacttcgt attgaatggc
     1921 ttcgctatgc ccatctggca cattactgta aaattct
//
LOCUS       DAKTLH010000252         1956 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.259, whole genome shotgun sequence.
ACCESSION   DAKTLH010000252 DAKTLH010000000
VERSION     DAKTLH010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.259"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..216
                     /locus_tag="O0394_002774"
     CDS             <1..216
                     /locus_tag="O0394_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500787.1"
                     /translation="KSVTYKYFPNNDENIKPGIIQMDIDSLEVINAEKSSLEKNTRDN
                     YFIHAIDRIYINTSKGLFPESELVAWG"
     gene            251..373
                     /locus_tag="O0394_002775"
     CDS             251..373
                     /locus_tag="O0394_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500788.1"
                     /translation="MKVIFYCPKRADDILKASMEYQSTDYKRRYISWKTMIAMK"
     gene            349..447
                     /locus_tag="O0394_002776"
                     /pseudo
     CDS             349..447
                     /locus_tag="O0394_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head morphogenesis protein"
     gene            756..893
                     /locus_tag="O0394_002777"
     CDS             756..893
                     /locus_tag="O0394_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500789.1"
                     /translation="MILIMLDSQLDGNVTAKELSESFKELVEEFESIEKAKKNSNSKD
                     K"
     gene            955..1215
                     /locus_tag="O0394_002778"
                     /pseudo
     CDS             955..1215
                     /locus_tag="O0394_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282612.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            1264..1612
                     /locus_tag="O0394_002779"
                     /pseudo
     CDS             1264..1612
                     /locus_tag="O0394_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456552.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1623..1817
                     /locus_tag="O0394_002780"
     CDS             1623..1817
                     /locus_tag="O0394_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500790.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGFVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
BASE COUNT          821 a          207 c          332 g          596 t
ORIGIN      
        1 aaaagtgtta catataaata ttttcctaat aatgatgaaa atattaagcc tggtattatt
       61 caaatggata tagatagtct tgaagttatc aatgctgaaa aatctagttt agaaaaaaat
      121 acaagagata attattttat tcatgccata gacagaatat acataaatac tagtaaaggg
      181 ctatttcctg aatctgaact cgtagcatgg ggataaagaa agtttacttt aaaaatttta
      241 accttcaaat gtgaaggtta ttttttattg cccaaaacgt gctgatgaca ttttaaaagc
      301 aagtatggaa tatcagtcga cagactataa acggaggtat atctcatgga aaacaatgat
      361 agcaatgaag tagcttattt gttaaaagat ggtaaattta caaaagtata tgtcaatcaa
      421 gatagtgtat cttttgtgcc agggtaacaa gcaaccgaat tattatttaa cagtaaaccg
      481 aattcaattg ttatgttaca taaccatcct ggacagtcag gattttcaga atatgattta
      541 tttacatttt ttaagcatcc atcaataaaa tcaatgacaa tcgttactaa taagggacaa
      601 gtaaaattta ttaccaagtc aaataggttt catggtaaaa tagtgagtaa attttgtgct
      661 aaatatttta cgcatattaa tatcattaat gatagccata ttgaaaaatt attgaaaaaa
      721 ctttatagta taaatatgat taaatataaa gtgagatgat tttaattatg ttagattcac
      781 aattagatgg aaatgtaact gcaaaagaat tgagtgaatc ttttaaagaa ttagttgaag
      841 aatttgaaag tatagaaaaa gcgaaaaaga attcaaattc aaaagataaa tagcatcctt
      901 tttagacaga taaagagaaa gtggtgctat ttttatacgc ttttttaact atttactgaa
      961 aatgcagaag cacaaaagtt aacatattta aaagaacttg gtgaagatgg cgaatataaa
     1021 tatgttgcca aaatagatag taaaacatct aaattatgtc attcacttaa cggaaaaata
     1081 tttaaagtta aagatatgat accaggtgtg aatgcgccac ctatgcatcc ttggtgtaga
     1141 agtaccacag tgccacatgt tggcaattgg cgggacaagt tctttaaaga gcgtgaaggt
     1201 aaatatcaag tagaagtaaa agaagcataa ttacaggaaa aagctaaaaa ccagatgaaa
     1261 gaaatgatta aaaatggtaa aataaaaata gaaataaatt gcggaaaaat aaaatagata
     1321 tatgttaggt catcacctat ataatgaaaa taaaaaaaga gccattttaa ataataagaa
     1381 attgcctagc tatacaatac tttctataga tttattgaat gaattgttaa gagaaaaaat
     1441 gtcaacaggc aatctaatat taagtgatga gcgatttgat ttgaaagaga ttattaattt
     1501 taatcaaatt attggaaaaa tacatatcga aaatgtgtat attgaaacca gaaagggaaa
     1561 agtgcattat tcgaagacag gtgctcatat agtaccttat attgataagt aggtgaaaag
     1621 tattgagaat tgaagatgca tatcgtaaag atgttattat cacgcttttg aataatgaag
     1681 aatacgaagg gtttgtaact gactatgaaa atgaattcga gagcgaaaca ggaaatcttg
     1741 ttgtagatat acagactgat tttgctgttt attcgtttga tgaaactgag ataaaaagta
     1801 ttagattatt aaaataatta caaaatagca tcctttctac aaaaagctaa aaaagaaatg
     1861 cttgaaatga taagtaatgg taaaatgacg atgaaattga ataatgttaa gcaaaagcgt
     1921 catattttat gcacaaatga atataataat aagaaa
//
LOCUS       DAKTLH010000253         1929 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.260, whole genome shotgun sequence.
ACCESSION   DAKTLH010000253 DAKTLH010000000
VERSION     DAKTLH010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.260"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..968)
                     /locus_tag="O0394_002781"
     CDS             complement(<1..968)
                     /locus_tag="O0394_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCY0500791.1"
                     /translation="MSNTQKKNVPELRFPGFEGEWEEKKLGNLTTKIGSGKTPKGGSE
                     NYTNKGIPFLRSQNIRNGKLNLNDLVYISKDIDDEMKNSRTYYGDVLLNITGASIGRT
                     AINSIVETHANLNQHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFK
                     EIANLKIFTPTIFEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFTQELRF
                     KDENGEEYPEWENKFIKDIFIFENNRRKPITSSLREKGLYPYYGATGIIDYVKDYLFN
                     NEERLLIGEDGAKWGQFETSSFIANGQYWVNNHAHVVKSNDHNLFF"
     gene            complement(961..>1929)
                     /locus_tag="O0394_002782"
     CDS             complement(961..>1929)
                     /locus_tag="O0394_002782"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCY0500792.1"
                     /translation="GKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLR
                     VGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAV
                     IANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPH
                     GVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLF
                     IDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRY
                     VDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE"
BASE COUNT          522 a          382 c          288 g          737 t
ORIGIN      
        1 ataaaaaaca aattatgatc attactttta actacatgcg catgattatt tacccagtat
       61 tgcccattag caataaagct actcgtctca aactgccccc attttgcacc atcttctcct
      121 attagtaatc gttcttcatt attgaataaa taatctttta cgtaatcaat aattccagtt
      181 gcaccatagt aagggtataa ccccttttct cttaatgaag aagtaattgg ttttcttcta
      241 ttattttcaa agataaagat gtcttttatg aatttgtttt cccactctgg atattcttca
      301 ccattctcat ctttgaatcg caattcttgt gtgaagattt tctgcatata gccttttttc
      361 tgttgttgaa gcaattcaag tttttgttct tctaattcaa tttgtcggtc gagtttgctg
      421 aagaacttgc ctattttttg ctgttcttca aatatagttg gggtgaagat ttttaaatta
      481 gcaatttctt tgaagtttag accttctcga ctacctccac tttgtgcaag gaaaattttc
      541 cttttacctt ttcttgataa tagatactgt ccaaaaaaat tataataata ctcttttttc
      601 aatctaataa tacatacatg ttgatttaaa ttagcatgcg tttcaactat cgaattaatg
      661 gctgttctac ctattgatgc tcctgtaata tttaaaagaa catcaccata gtacgttcta
      721 ctatttttca tctcatcatc tatatcttta ctaatataaa ctaagtcatt aagatttaat
      781 ttaccatttc taatattttg actccttaaa aatggtatgc ctttgtttgt atagttttca
      841 cttccacctt tgggagtctt tccactacct attttggtag taagattccc taacttcttc
      901 tcttcccatt cgccttcaaa tcctgggaat ctcaattctg gcacattttt cttttgtgta
      961 ttactcatct ttcaacaccc caagttcttt caggtatgca ttgatttctt gctcaatttc
     1021 tgcgatttct ttatcgatat ttttcaaatc ttgttggact tgatctaaat caatcggtgc
     1081 ttcttcttcg aatgtatcga catatctcgg tatatttagg ttgtaatcgt tatcggcaat
     1141 ctcttgtagt gtcgcgctgt agctatattt atcaattgtt tccttacgct tatatgtgtc
     1201 tataatacgt tcgacttggg catcgcttaa atgattttga ttttttcctt tttcaaaatc
     1261 attggatgca tcgataaata gtacgttgtc gtcttgttgg cgacattttt taaatactaa
     1321 aatacatgtt ggaatacttg tcccatagaa aatattcgct ggcaaaccaa tcacagcttc
     1381 taagtagttc ttttcttcaa ttaaataacg acgaatgaca ccttctgcag cacctcggaa
     1441 taatacacca tgtgggagta caacggccat ggtaccttca tcgtctaggt aatgtaccat
     1501 gtgttgaata aaggcaaagt ctgctttaga cttaggcgca agtttgccgt aaccactgaa
     1561 tcgttcgtca ttttcaaact ttgaatctgc agtccatttc gcactatacg gtgggttcgc
     1621 aataaccgca tcaaatgtat tgcctaaaaa ggctgggttt tccaatgtgt catcattacg
     1681 gatatcgaag ttctcataac gcacatcatg taataacata ttcatgcgtg ctaagttgta
     1741 tgtagtattg ttacgttctt gaccgaaata acgatacact tgtgtttctt taccaacacg
     1801 taacaacagt gaacctgaac cacatgttgg gtcatacacg tgacgtaatt tatctttacc
     1861 gtctgtgaca atcttcgcca gtatcttaga tacttgttgt ggtgtataga actcgcctgc
     1921 ttttttacc
//
LOCUS       DAKTLH010000254         1864 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.261, whole genome shotgun sequence.
ACCESSION   DAKTLH010000254 DAKTLH010000000
VERSION     DAKTLH010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.261"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            31..774
                     /locus_tag="O0394_002783"
     CDS             31..774
                     /locus_tag="O0394_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500793.1"
                     /translation="MDLLQIEKKGNLTKTVQLMHDEKGSASGESKVNMEFGKQSAVKQ
                     IFDSLLGMSASASASAGINGNITGERVAKTILENTLIYDFLDTVEFRKKKTIIDISEG
                     FKLNIQQDTMTYFATITPIASMFEGSKHLDEEELSFDVSKMNEAIRSIKGYFELIGTR
                     GNENRVFRFNIESFKNNYRIQDLKFMNLTLYSVLVGEISKENLNYDKEFDLNQGAKPS
                     GIGKFKGIRSEATKEDKYQVYDVILAGVK"
     gene            775..>1864
                     /locus_tag="O0394_002784"
     CDS             775..>1864
                     /locus_tag="O0394_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCY0500794.1"
                     /translation="MEIHVYTGPLCGFNEYINSEVKEYHRFMELIREYNIIVRASDTF
                     GTHDVDGIFDTEIENLVIFSNDFASVTTHVITNFINIVTLGRNIKKIYIQNPPKRVFQ
                     SLNSFHDSEIIEINYEYTTLKKDDVINFYKEQMAGSKIIGQTDAKKQMSIDLYKHTSL
                     ENNKPTVMLYYGPSGVGKTELAKEIAKKYKGNITRIQFSMMQNDESFNYIFGDEHAKV
                     SLSKDLVDRETNVVLIDEFDKVLPIYYNVFYQMFDEGIIEDANYKVDVSNCIFILTSN
                     FNSIEEAIVNIGEPVFSRIDSCIKFLYLTQEEKQKVIENKLNEILSSLNEKEKRIVTA
                     YNLLEKYKETEIDIDNIRYLKKIITNDIY"
BASE COUNT          759 a          205 c          320 g          580 t
ORIGIN      
        1 aaaataattt attttgatga actatcagca atggatttat tacagataga gaagaaaggt
       61 aaccttacta aaacagttca gttgatgcat gatgaaaaag gtagtgcaag tggtgaatct
      121 aaagtgaaca tggaatttgg taagcaaagc gctgtaaaac aaatttttga ttctttactt
      181 gggatgtcag cgagtgcttc ggctagtgca ggtataaatg gaaatataac tggagagcga
      241 gtagccaaaa caattttaga gaatacatta atatatgatt ttttggatac agtagagttt
      301 agaaagaaaa aaactattat tgatatttca gaggggttta aactaaatat tcaacaagat
      361 acgatgacat atttcgcgac tattaccccc atagcaagta tgtttgaagg atctaaacat
      421 ctagacgagg aggagctatc atttgatgtt tcaaaaatga atgaagcgat tagaagtatt
      481 aaaggctatt ttgaattaat aggaacaaga gggaatgaaa acagagtttt tagatttaat
      541 atagaatctt ttaaaaataa ctatagaata caagatttaa aatttatgaa tttaacactc
      601 tactcagtac ttgttggtga aataagcaaa gaaaatctaa attacgataa agagtttgat
      661 ctaaatcaag gtgccaaacc tagtggaatt ggaaagttta agggaattag atcagaagca
      721 actaaagaag ataaatatca agtgtatgat gttattcttg caggagtgaa ataaatggaa
      781 attcatgttt atacgggacc gctttgtgga tttaatgaat atattaattc tgaagttaaa
      841 gaatatcacc gcttcatgga attaatcaga gaatacaata tcatagtgcg agcaagtgat
      901 acatttggaa ctcatgatgt tgatggtatt tttgatactg agatagaaaa tcttgtgata
      961 ttttcaaacg actttgcttc tgtaacaact catgttatta ctaattttat aaatatcgtt
     1021 actttaggta gaaatattaa gaaaatctat attcagaatc ctccaaaaag agtatttcaa
     1081 tcactaaata gttttcacga ttctgaaata atagaaataa actatgaata tacaacatta
     1141 aaaaaagatg atgtaataaa tttttataaa gagcagatgg ctggttctaa aataataggt
     1201 caaacagacg ctaaaaaaca aatgagcata gatttatata aacatacttc cttagaaaat
     1261 aataaaccga ccgtaatgct ttactatggt ccatcaggtg ttggaaaaac agaattagcc
     1321 aaagaaatag caaaaaaata taaaggtaat atcactagaa tacaattttc tatgatgcag
     1381 aatgatgaat cttttaatta tatctttgga gatgaacatg ccaaagtatc attgtcaaaa
     1441 gatttagtag atcgtgaaac taatgttgtt ttaattgatg aatttgataa ggtattacca
     1501 atctattaca atgttttcta tcaaatgttt gatgaaggta ttattgaaga tgccaattat
     1561 aaagtagacg tttcaaattg tattttcatt ctaacatcta attttaatag tattgaagaa
     1621 gcaatagtta atattggaga accggtattt tctagaatag attcctgtat taaattttta
     1681 tatttaacac aggaagaaaa gcagaaagta atagaaaata aattaaatga aatactatca
     1741 tctctaaatg aaaaagaaaa aagaatagtt accgcatata acttgttaga gaaatataaa
     1801 gagacagaaa tagatatcga taacattagg tatttaaaga aaataataac aaatgatatt
     1861 tata
//
LOCUS       DAKTLH010000255         1849 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.262, whole genome shotgun sequence.
ACCESSION   DAKTLH010000255 DAKTLH010000000
VERSION     DAKTLH010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.262"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            9..1100
                     /locus_tag="O0394_002785"
     CDS             9..1100
                     /locus_tag="O0394_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0500795.1"
                     /translation="MIDFFKDNKRIERFSFIDNKVHMKETFNVDNKVCYQVFYDEKGY
                     PYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDSKDSIMICDGPGSFP
                     KMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKINGVIVLTEAQRLDI
                     LNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRIDLLIEVAELVVKKDN
                     AVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLEDFKLVVSTSQYEGQG
                     LSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNINDMADKILQLVNNDVLAA
                     EFGSKARENIIEKYSTESILEKWLNLFNS"
     gene            complement(1189..1545)
                     /locus_tag="O0394_002786"
     CDS             complement(1189..1545)
                     /locus_tag="O0394_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCY0500796.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
BASE COUNT          739 a          221 c          302 g          587 t
ORIGIN      
        1 ctgaaaaagt gattgatttc tttaaagata ataaacgaat tgaacggttt agttttatag
       61 ataataaagt gcatatgaag gaaacattta atgtagataa taaagtttgt tatcaagtat
      121 tttatgatga aaagggatac ccatatattt caaggaatat taatgctaat aatggtgctg
      181 taggtaaaac ttatgtgtta gttaataaaa aagaatttaa aaacaattta gcactgtgtg
      241 tttactattt agaaaaacta ataaaagatt ctaaagatag tattatgatt tgtgatggac
      301 cagggagttt tccaaaaatg tttaatacaa atcataaaaa tgctcagaaa tatggcgtta
      361 ttcatgttaa tcatcatgaa aatttcgatg atacgggtgc atttaaaaaa agtgagaaat
      421 atattattga gaatgcgaat aaaattaacg gtgtaattgt attaacagag gcacaaagat
      481 tagatattct taatcaattt gatgtagaaa atattttcac tattagcaat tttgttaaga
      541 tacataatgc tccaaaacat tttcaaactg aaaaaatcgt aggtcatatt tctagaatgg
      601 taccaacgaa gcgaattgat ttgcttattg aagtggctga gttagtcgta aaaaaagata
      661 atgctgttaa atttcatata tatggagaag gatctgtcaa agataaaata gctaaaatga
      721 ttgaagataa aaatttagaa agaaatgttt ttcttaaagg atatacaaca actccacaaa
      781 aatgcttgga agattttaaa ttagtcgttt ctacatctca atatgaaggt caagggttaa
      841 gtatgataga agcaatgatt tctaaaaggc ctgttgttgc ctttgacatc aaatacggac
      901 caagtgattt tatagaagat aataaaaatg gttatttaat agaaaaccat aatattaacg
      961 acatggctga taaaatactt cagcttgtta ataatgatgt attagcagcg gagtttggtt
     1021 cgaaagcgag agaaaacatt atagaaaaat attcaacgga atcaatatta gaaaaatggt
     1081 taaatctttt caatagctaa atgaaaaaat cctaggtatt catgagtacc taggattttt
     1141 acgcatgata aatacaaaaa aagagaggat agtcctctca aacaatagtt aataaaattg
     1201 tagccatttc ttccagctgt aactactaat acaagctaag ttcattacga tatgtatgat
     1261 ttcaaattca taaccactta catatcctct tgtaagttta agtgtgaaga ttgagcctaa
     1321 cataacacaa atataaaaaa cattaacata aggcgttaaa aagccgataa taagtgctaa
     1381 acctccaaca acttcaccaa ttgacatcaa tactgcgatg gtaggtggta aacctaaaga
     1441 gcctaagaag tgaattgttc catctaaatc aacaaatttc aaagtaccat gcactaagaa
     1501 tgatgttgct aacatccatc taattaaaag catacctaat ttcatcatgt cacctccaac
     1561 attcatattt ttaaaacata gtccaaatta tatttgtaaa catgtggaaa gtaaagattt
     1621 tttataagat taattattaa atattctatt gtattgaaca attgttgtag aaagatgttt
     1681 aagtcatcag ttactttttg tatttataaa taaagattag tacagcatag tgcaaagatt
     1741 atagggaaat gaattaacta ttgcatcgct atttatgaat cgtaaacact attatgaaat
     1801 tgcgagagta gtatggacaa ttagaatgat ggtttgagat aaatgagtg
//
LOCUS       DAKTLH010000256         1828 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.263, whole genome shotgun sequence.
ACCESSION   DAKTLH010000256 DAKTLH010000000
VERSION     DAKTLH010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.263"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            118..1828
                     /gene="sasG"
                     /locus_tag="O0394_002787"
                     /pseudo
     CDS             118..1828
                     /gene="sasG"
                     /locus_tag="O0394_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
BASE COUNT          751 a          252 c          371 g          454 t
ORIGIN      
        1 aaaataattt tataaagata agtgttatat tccattgtgc aactcctatt gtatttgtaa
       61 aattacatgt aaattcactg taagcaaagt ggaaaatatg gaaaaaggag tatgcaaatg
      121 agagataaga aaggaccggt aaataaaaga gtagattttc tatcaaataa attgaataaa
      181 tattcaataa gaaaatttac agttggaaca gcatctattt taattggctc actaatgtat
      241 ttgggaactc aacaagaggc agaagcagct gaaaacaata ttgagaatcc aactacatta
      301 aaagataatg tccaatcaaa agaagtgaag attgaagaag taacaaacaa agacactgca
      361 ccacagggtg tagaagctaa atctgaagta acttcaaaca aagacacaat cgaacatgaa
      421 ccatcagtaa aagctgaaga tatatcaaaa aaggaggata caccaaaaga agtagctgat
      481 gttgctgaag ttcagccgaa atcgtcagtc actcataacg cagagacacc taaggttaga
      541 aaagctcgtt ctgttgatga aggctctttt gatattacaa gagattctaa aaatgtagtt
      601 gaatctaccc caattacaat tcaaggtaaa gaacattttg aaggttacgg aagtgttgat
      661 atacaaaaaa aaccaacaga tttaggggta tcagaggtaa ccaggtttaa tgttggtaat
      721 gaaagtaatg gtttgatagg agctttacaa ttaaaaaata aaatagattt tagtaaggat
      781 ttcaatttta aagttagagt ggcaaataac catcaatcaa ataccacagg tgctgatggt
      841 tgggggttct tatttagtaa aggaaatgca gaagaatatt taactaatgg tggaatcctt
      901 ggggataaag gtctggtaaa ttcaggcgga tttaaaattg atactggata catttataca
      961 agttccatgg acaaaactga aaagcaagct ggacaaggtt atagaggata cggagctttt
     1021 gtgaaaaatg acagttctgg taattcacaa atggttggag aaaatattga taaatcaaaa
     1081 actaattttt taaactatgc ggacaattca actaatacat cagatggaaa gtttcatggg
     1141 caacgtttaa atgatgtcat cttaacttat gttgcttcaa ctggtaaaat gagagcagaa
     1201 tatgctggta aaacttggga gacttcaata acagatttag gtttatctaa aaatcaggca
     1261 tataatttct taattacatc tagtcaaaga tggggcctta atcaagggat aaatgcaaat
     1321 ggctggatga gaactgactt gaaaggttca gagtttactt ttacaccaga agcgccaaaa
     1381 acaataacag aattagaaaa aaagttgaag agattccatt caagaaagaa cgtaaattta
     1441 atccggattt agcaccaggg acagaaaaag taacaagaga aggacaaaaa ggtgagaaga
     1501 caataacgac accaacacta aaaaatccat taactggagt aattattagt aaaggtgaac
     1561 caaaagaaga gattacaaaa gatccgatta atgaattaac agaatacgga cctgaaacaa
     1621 tagcgccagg tcatcgagac gaatttgatc cgaagttacc aacaggagag aaagaggaag
     1681 ttccaggtaa accaggaatt aagaatccag aaacaggaga cgtagttaga ccgccggtcg
     1741 atagcgtaac aaaatatgga cctgtaaaag gagactcgat tgtagaaaaa gaagagattc
     1801 cattcgagaa agaacgtaaa tttaatcc
//
LOCUS       DAKTLH010000257         1824 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.264, whole genome shotgun sequence.
ACCESSION   DAKTLH010000257 DAKTLH010000000
VERSION     DAKTLH010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.264"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..490
                     /locus_tag="O0394_002788"
     CDS             <1..490
                     /locus_tag="O0394_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCY0500797.1"
                     /translation="YNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGIT
                     LQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKR
                     GKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQS
                     IK"
     gene            504..815
                     /locus_tag="O0394_002789"
     CDS             504..815
                     /locus_tag="O0394_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCY0500798.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVA
                     N"
     gene            793..1239
                     /locus_tag="O0394_002790"
     CDS             793..1239
                     /locus_tag="O0394_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCY0500799.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISFITELNYIKSQAIANQGYINVRFYENSDTIKVIENNNIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITIYNNNSIYRIIFHIEKGRIRYEKL"
     gene            1226..1525
                     /locus_tag="O0394_002791"
     CDS             1226..1525
                     /locus_tag="O0394_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HCY0500800.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            1443..>1824
                     /locus_tag="O0394_002792"
     CDS             1443..>1824
                     /locus_tag="O0394_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCY0500801.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDILDDFKGVDRNDIEIRQHRIILH
                     KGEEMIEYKLINNKIIKVVNDRGNI"
BASE COUNT          725 a          205 c          289 g          605 t
ORIGIN      
        1 ttataacaat ttaaatatgc tcaataagat aaactttgtg atgaaactac cgctaatatc
       61 aggctatttc caattattta aaacttattt tgtaactaat gaattagtgt tgttttataa
      121 aaatggtatt acacttcaat caatagtaga cgtttatatt aaccatagta gtgatccatt
      181 tagacagttt ctaggtaaat acttattaac ttattcagaa atgggatatg gtttgcctca
      241 aattttagaa aaactaaaat gctttaagcc tcaattaatt aagtttgtgc tacaaggtga
      301 aaagagaggg aagctagaag tagaactaaa gttatattcg caaatattag taaaacaaat
      361 agaagataaa gcgataaaac agactcagtt tttacagcct attttatttt tgattttagg
      421 tttatttatt gtcgcaattt atttagtaat tatgttacca atgtttcaaa tgatgcaaag
      481 tataaaataa tggaggttaa tatatgttta aatttcttaa gaaaactcaa gcgtttacat
      541 tgatagagat gctattagtg ttattaatca tcagtttatt attaatttta atcattccaa
      601 atattgctaa acaaactgct cacatacaat caacaggttg taatgcacag gtaaaaatgg
      661 ttaatagtca aattgaagcg tatgcattga aacataatag aaatccatcg tctattgaag
      721 acttaattgc agatggtttt ataaaagaag cacaaaagac atgtaaatca ggagagacaa
      781 tcacaattag taatggagaa gcagttgcaa attagaaagc agtcagcatt tactatgatt
      841 gagatgcttg tggtaatgat gttaatcagt atatttctac ttttgacaat gacatctaaa
      901 ggattaagca atcttagagt aatagatgat gaggcaaata tcatttcttt tattactgaa
      961 ttgaattata ttaagtcgca agctatagca aatcaaggat atatcaatgt tagattttat
     1021 gaaaacagtg acactattaa agtaatagag aataataata tacgatttct aaaattaaaa
     1081 gtaggcaaaa taattaatgt tgcaaaagtt gatattattg cctttgataa aaaagggaat
     1141 atcaataaat ttggtagcat aacaatttac aataacaatt caatttatag aataatattc
     1201 catattgaaa aaggaagaat tcgttatgaa aagctataag tgtaaaggtt cattcttaat
     1261 agatagtatg gctggatttt tgctaattgg attgattaca ttactattga taccaatgat
     1321 gaatcaaatg caagcgagta taaaccataa actacaaaca attgatgctt ctaaagtaat
     1381 tttgacgact gtatctaaaa ttaataaaga agaacttaag aagggggtaa ctatagggaa
     1441 gtatgatatt aagcaaagtg accaacaaat ttgtgctatt tcaaaaaata ccacttctta
     1501 tcaaaagaca tgtatacagt attaatgtca aagctttttc gctcattgaa atgttagtag
     1561 cgatgatggt tataagtata actttactaa ttgttccaga cttaattaga cttagtaaaa
     1621 cttttctaat tgaaagtagg gatttaacaa ctgtagattt cgaatttttc tcaagagata
     1681 ttctagatga ttttaaagga gtagatagaa acgatattga aattaggcaa caccgtatca
     1741 ttttacataa aggtgaagaa atgatcgaat acaaattaat taataataaa attattaaag
     1801 ttgtaaatga cagaggaaat ataa
//
LOCUS       DAKTLH010000258         1823 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.265, whole genome shotgun sequence.
ACCESSION   DAKTLH010000258 DAKTLH010000000
VERSION     DAKTLH010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.265"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            151..1641
                     /locus_tag="O0394_002793"
     CDS             151..1641
                     /locus_tag="O0394_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HCY0500802.1"
                     /translation="MHRESKYIEYKKSRKGLSNDIWSTYSAFANTEGGTIYLGIEEKK
                     IEDKKVFVSVGVEDPEKMIEDFWNALYGRSKVSQNILSNKDVKIVNIENKACIEIHVP
                     EAPYSKKPIYVDNKKDLVYKRVDDADRIATEEEYKFMIVNSQDDIDTELLDNYDMSDL
                     NHESIENYRKLLLKNTNDERYANMSQLDLMIDLGAYRKDRSSKDKQYKMTTACLLFFG
                     KYNAISDRFPGFQLDYFKKTNYLDTDWKDRISSGDLGNEDLNVYSFFEKVLIKLTDNI
                     EESFSLNDGLTRQNYARDLKVAIREALVNTLMHAYYDTKQSIKIVNCEDFIEFYNPGN
                     MRINKEDFIHGGHSKDRNSILSTLFRRVGYSEKAGSGGPRIFDVVNRHKLKTPEIELT
                     DMDTNVVLWKQDLMKEFEKYPELDKKVIKYIIDYGSISKGEALKMENMTEYQFRNILK
                     KLKDDNLIKKEGEGPATKYVLIESKEADILRTKKVIKSLESFFRNK"
BASE COUNT          737 a          179 c          362 g          545 t
ORIGIN      
        1 cgtagtaatc gcgcgaaaat cgcgtgaaat aatcagagga atcgtttgaa atcatcgtag
       61 taatcgcgcg aaaatcgcgt gaaatactat ggtagacggt ttgagtaaat taatggagta
      121 ttttaatatt tatggtttgg aggaattgcg ttgcatagag aaagtaaata tatagagtat
      181 aagaaatcac gaaaaggatt atctaatgat atttggtcta cgtatagtgc ttttgcaaat
      241 actgaaggtg gtactatata tttaggaatt gaagaaaaaa agatcgagga caaaaaagtc
      301 tttgtttcag ttggtgttga agatccagag aaaatgattg aagatttttg gaatgcacta
      361 tatggaagaa gtaaagttag tcaaaatatt ttatcaaata aagatgttaa aattgttaat
      421 attgaaaata aagcgtgcat tgaaattcat gtaccagaag cgccttattc gaagaaaccg
      481 atatatgtag ataataaaaa agatttagta tataaaagag ttgatgatgc tgatagaatt
      541 gcgactgaag aagagtataa attcatgatt gtaaattctc aagacgatat agatacagaa
      601 ttattagata actatgacat gtctgattta aatcacgaat ctatcgaaaa ttataggaaa
      661 cttctattaa aaaatactaa tgatgagaga tatgcgaata tgagccaact ggatttaatg
      721 atagatttag gagcatatag aaaagataga agttcgaaag acaaacagta taaaatgact
      781 acagcatgtt tattattctt tggtaagtat aatgcgatta gtgatagatt cccaggattt
      841 caattagatt attttaagaa aacaaattac ctagatactg attggaaaga tagaatatca
      901 agtggagatt taggtaatga agatttaaac gtgtatagtt tttttgaaaa agtattgata
      961 aaattaactg ataacattga ggaatcattt agcctaaatg atggtttgac tagacaaaat
     1021 tatgcaagag atttaaaagt agcaattcgc gaagcactgg ttaatacatt aatgcatgcg
     1081 tattatgata ctaagcaaag tattaaaata gttaattgtg aagattttat agagttttat
     1141 aatccgggta atatgagaat aaataaagaa gattttattc atggagggca ttcaaaggac
     1201 agaaatagta tattatcgac gcttttcaga agagtaggat attcagaaaa agctggatct
     1261 ggaggaccaa ggatattcga tgtagttaat agacataagc ttaaaacgcc tgaaatagaa
     1321 ttaacggaca tggacactaa tgtagtactt tggaaacaag atttaatgaa ggagtttgaa
     1381 aaatatcctg agttagacaa aaaagtaata aagtatatta ttgactatgg atcaataagt
     1441 aagggtgaag ccttaaaaat ggaaaatatg acagaatatc agtttagaaa tattttaaaa
     1501 aaactaaaag atgataactt gataaaaaaa gaaggtgaag gtccggctac taaatatgtg
     1561 ttaatagaat caaaagaagc tgatatattg cgaactaaaa aagtaattaa aagtttagag
     1621 tctttcttta ggaataaata aaaaaacaag agataggtgc gaagtgtttg gattatacaa
     1681 atgcttcgca tcttatattt attaataaat atcatagaaa accgtatcat taaccgatac
     1741 gcagagatgc ggttttttag acacttcata aagggatttt gaacgtatca gaacatatga
     1801 ggtttatagg aattgctgtt atg
//
LOCUS       DAKTLH010000259         1809 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.266, whole genome shotgun sequence.
ACCESSION   DAKTLH010000259 DAKTLH010000000
VERSION     DAKTLH010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.266"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..896
                     /locus_tag="O0394_002794"
     CDS             <1..896
                     /locus_tag="O0394_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HCY0500803.1"
                     /translation="EIVTPIYKKSSYRGPEIEIPKYKNTDIEYDKDWFALHIHIDKPS
                     QDTFIIDNLYPFVKHLKDKGDIDQYFLMRYIKQGDILKLRLFRNDENYAEIYSILKNW
                     LPHVRQTTEVSDYEFVSYEPEFFRYGGKNTINEIEAFFEYDTNLAVNIIENDFKFDRP
                     YIVAISIMYLFEMFSISNEERMEIVNNYVPTSFKSKDIRPFKNELVTICNPANNFEYM
                     AKHYSGIYRILKDGNQILSKLNEGLKKTLTTKRSRIIGSLIHMRCNRIFGIDKDQETF
                     VLSIVKEIVKTQKHWCGDKND"
     gene            889..>1809
                     /locus_tag="O0394_002795"
     CDS             889..>1809
                     /locus_tag="O0394_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HCY0500804.1"
                     /translation="MIDMNLILKKKFQEISEVDDFISKASTETDYFEPSTLSHGIPGI
                     ILFLDAYQKVFDINTEQIVHKYIMKLAPYLQSGQYNNSLFGGLSGIAFSMDLASQNGK
                     NYQNILNNIDGHIVNEIENKFDQILQEPLNPLNYDTVSGLAGIGRYLLNRVDENEFNV
                     KALKSILVYFKDIQYSKNSWVVPQESQFLESDKNYFTEGNINLGLAHGVLGPMSLFAL
                     CVIKGITIENHQHILKDMYKFIMDEKFCNHERWLQRYDLISERNHFNFIRNGWCYGNT
                     GVMTTLFLIGQALQDDEIIKMSKKVMLQVVN"
BASE COUNT          682 a          224 c          305 g          598 t
ORIGIN      
        1 atgaaattgt tacaccaatt tataaaaaat catcttatcg aggacccgaa atcgaaattc
       61 caaaatataa aaatacagat attgaatatg acaaagactg gtttgcatta catattcata
      121 tcgataagcc atcgcaagat acatttatca tcgacaattt gtatccattt gtaaaacatc
      181 ttaaagataa gggagatata gatcaatatt ttttaatgcg ttatatcaaa caaggtgaca
      241 ttttaaagtt gaggttattc aggaatgacg aaaattacgc agaaatatac agcatcttga
      301 agaattggtt accacatgtt cgacaaacga cagaagtttc agattatgaa tttgtatcat
      361 acgagccaga attttttaga tatggtggaa agaacacgat taatgaaata gaagccttct
      421 ttgaatatga cacgaattta gctgtcaata ttattgaaaa tgattttaag tttgatcgac
      481 catatattgt tgctatttca ataatgtatt tatttgagat gttttcaatt tctaatgaag
      541 agcgtatgga aattgttaat aactatgtac ctacatcatt taaaagtaaa gatattagac
      601 cttttaaaaa tgaacttgta actatatgta atcctgctaa taattttgaa tatatggcta
      661 aacactatag tggtatttat cgaattttaa aagatggcaa ccaaattttg agcaaattaa
      721 atgaagggct taaaaaaact ttaactacta aaaggtcaag gattattggc agcttaattc
      781 atatgcgctg caatcgaata tttggcattg ataaggatca agaaacattt gttttatcta
      841 ttgtaaaaga aattgttaaa actcaaaagc attggtgtgg tgacaaaaat gattgatatg
      901 aatctaatat tgaagaagaa gtttcaagag atttcagaag tagatgattt catttcaaaa
      961 gcatctactg aaaccgatta ttttgaacct tcaactttat cacatggtat accaggtatc
     1021 attttgtttt tagatgctta tcaaaaagtt ttcgatatta atactgagca aattgtgcat
     1081 aagtacatca tgaaattagc accatattta cagagtggtc aatataacaa ttctttgttt
     1141 ggtggacttt caggtattgc attttcaatg gatttagcat cgcaaaatgg caaaaattat
     1201 caaaatattt taaataatat tgatggtcac attgtaaatg aaatagaaaa taaatttgat
     1261 caaatattac aagaaccatt aaatccatta aattatgata ctgtcagtgg attagctggg
     1321 atagggagat atttgctaaa tagagtagat gagaatgaat ttaatgttaa agcattaaaa
     1381 agcatattag tatactttaa agatattcaa tattctaaaa atagctgggt agtcccacaa
     1441 gaaagtcaat ttttagagtc tgataaaaat tattttactg aaggtaatat caatcttggc
     1501 cttgcacatg gagtgctagg accgatgtct ttatttgcat tatgcgtgat taaaggaatt
     1561 acgattgaaa atcatcagca catattaaaa gacatgtaca aatttatcat ggacgaaaaa
     1621 ttttgtaacc acgaaagatg gttgcagcgt tacgatttaa tttctgaacg taatcatttc
     1681 aattttattc ggaatggttg gtgttatggc aatacgggtg taatgacgac gttgttttta
     1741 atcggccaag cattacaaga tgatgaaata attaaaatgt ctaaaaaagt gatgctacaa
     1801 gtagtaaat
//
LOCUS       DAKTLH010000260         1787 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.267, whole genome shotgun sequence.
ACCESSION   DAKTLH010000260 DAKTLH010000000
VERSION     DAKTLH010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.267"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            29..1429
                     /locus_tag="O0394_002796"
     CDS             29..1429
                     /locus_tag="O0394_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0500805.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
BASE COUNT          588 a          261 c          338 g          600 t
ORIGIN      
        1 aaacatacaa gagggagtgt atgaattcat ggattttaat aaagagaata ttaacatggt
       61 ggatgcaaag aaagctaaaa aaaccgttgt tgcaaccggt atcggtaatg caatggaatg
      121 gttcgatttt ggtgtctatg catatacaac tgcgtacatt ggagcgaact tcttctctcc
      181 agtagagaat gcagacattc gacaaatgtt gactttcgca gcattagcca ttgcgttttt
      241 attaagacca attggtggtg tcgtatttgg tattattggt gacaaatatg gacgtaaagt
      301 tgtattaaca tctacaatta ttttaatggc attttcaaca ttaaccattg gattattgcc
      361 aagctatgat caaattggac tttgggcacc aatactatta ttgcttgcaa gagtactaca
      421 agggttttca acaggtggag agtatgcggg ggcaatgaca tatgttgccg aatcatctcc
      481 agataagcgt cgtaactcat taggtagtgg actagaaatt gggacattat caggttacat
      541 agctgcttca attatgattg ctgtattaac attcttttta acagatgaac aaatggcatc
      601 atttggttgg agaatcccat tcttactcgg tttattccta ggattattcg gcttatattt
      661 acgtcgtaag ctggaagaat caccagtttt cgaaaatgat gttgcaacac aaccagaaag
      721 agataacatt aactttttac aaatcatcag attttattac aaagatatat ttgtatgttt
      781 tgtagctgtt gtattcttca atgttacaaa ctatatggta actgcatatt taccaaccta
      841 tttagaacaa gttattaaat tagatgcaac gacaacaagt gtattaatta cttgtgtcat
      901 ggcaataatg attccattag cattaatgtt tggtaagtta gcggataaaa taggtgaaaa
      961 gaaagtattt ctaattggta ctggtgggct aacattattc agtatcatcg catttatgtt
     1021 attacattca caatcatttg ttgtaatagt aatcggtata tttatattag gatttttctt
     1081 atcaacttac gaagcgacaa tgccagggtc gttaccaacg atgttttaca gtcatataag
     1141 atatcgaact ttatcagtaa catttaatat ctctgtttcg atatttggtg gtacgacgcc
     1201 attagttgca acatggttag ttacgaaaac tggagatcca ttagcacctg cgtattattt
     1261 aacagcaatc agtgttattg gctttttagt tattacattc ttacatttaa gtacagcagg
     1321 aaaatctcta aaaggttcgt atccaaatgt agataacgag caagatagag cttattatgc
     1381 agaacatcca aaagaagcat tatggtgggt taaagaacgt aagaattaga gattttaata
     1441 aaaagtataa atcaatcgta tataagcact ttaaagctag taggttctgc taactttaaa
     1501 gtgcttttta aattgagaac tgtaattagc cgtaataaag tttttgtata tacataaacc
     1561 cccactgcaa tgattatcgc aatgggggaa agaggggact taaagcatat gtttagcttt
     1621 gaatacttaa aattctcttg ctattgaaat gttaggatgt aaatatgtct tagagtattt
     1681 tgtccaacgc aattaatatt gagactctaa ccttcaatat tattatagag aacacaaact
     1741 taaatagatt gggtgactta tttgtgtcag ttattgcgat tgcgata
//
LOCUS       DAKTLH010000261         1763 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.268, whole genome shotgun sequence.
ACCESSION   DAKTLH010000261 DAKTLH010000000
VERSION     DAKTLH010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.268"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            60..1025
                     /locus_tag="O0394_002797"
     CDS             60..1025
                     /locus_tag="O0394_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0500806.1"
                     /translation="MSISEYKNRYLESLYNTFKTELENERTRYFILDTNYTNSDDTEK
                     EFTWDVKQNNKIREGDLFIFRRPTNLSQIAQQFYFFGAGKIEKIERKNNVATAYISKP
                     LLFVDRVLKDNIENLKWEFKERIKEEWEQFFFKNKISNITKNDFLKLLSLSRNVVEIE
                     SNYINNDISLTFKQMADMATRIKKNIYYVGNHEAFVQAKGSAHFEFSKRIKQNYSYRC
                     AITGIKTKDFLVVTHIIPWHENEFIRLDPSNGICLSLFLAKAFKKGFITFSNSYRVVL
                     SKEAEKDAALYEELKIYENQKIELPDCQKPNLKYLDWHREHIFKN"
     gene            1405..1734
                     /locus_tag="O0394_002798"
     CDS             1405..1734
                     /locus_tag="O0394_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500807.1"
                     /translation="MARTYNVIKYVIKKNKDDLKIHGLRLYEFNIKDEKAINISQPIF
                     VNRATLIEKIQNGEAFTHAFRVNHNTYYAGQLISLSLNKNGYINNNFEASRDIIRNIE
                     VSILDYE"
BASE COUNT          716 a          189 c          287 g          571 t
ORIGIN      
        1 ttaataatta aataaaagta tgctaaatat ttataattta caagtgagct gatagaaaga
       61 tgagtataag cgaatataaa aatagatact tagagtcact atataacacc tttaaaactg
      121 agttagaaaa cgaacgcact cgctatttta tattagatac caactataca aatagtgatg
      181 atactgaaaa ggagtttact tgggatgtaa aacagaataa taagatcaga gaaggcgatt
      241 tgttcatttt tagacggcca actaatttat ctcaaattgc tcaacaattt tatttctttg
      301 gagctgggaa aatagaaaaa attgagcgaa aaaataatgt agcaaccgct tacatttcaa
      361 aacccttact ttttgtggat cgtgttttaa aagataatat tgaaaattta aaatgggagt
      421 tcaaagaaag aattaaagaa gagtgggagc agtttttctt taaaaataaa atcagtaata
      481 ttacaaaaaa tgatttttta aaactactaa gtttgagtag aaatgtagtg gaaatagaaa
      541 gtaattatat taataatgat atttcgctaa cttttaaaca aatggctgat atggcaactc
      601 gaataaagaa aaatatttac tacgtaggga atcatgaagc atttgttcaa gctaagggaa
      661 gtgctcattt tgagttttca aaaaggatta aacaaaatta tagttataga tgtgcaatta
      721 ccgggattaa aactaaagac tttttagtcg ttacacatat tattccttgg catgaaaatg
      781 agtttattag actagatcct tcaaatggta tttgtttgtc tttattttta gcaaaagcat
      841 ttaagaaagg ctttatcact ttttctaaca gttatagagt tgttttatct aaagaagcag
      901 agaaagatgc tgctttatat gaagaattaa agatttatga aaatcaaaaa atcgaactac
      961 cagattgtca gaaacctaat ttgaaatatt tagattggca tagagaacat atttttaaaa
     1021 attaattttg ggagaggttg tatgacagat attagtcaat tagtagaggt gccatacatc
     1081 tttcattagt agattgaaaa agagcacttg aaacagataa tttgtattat ttagcctatg
     1141 gattaacaac tttagataat tgttttaaat attaccaatt aattacacaa aatgaacatt
     1201 tttgctattc tcaacaagaa gaaagagaaa gttatcagca aaaatttcaa agtatgatta
     1261 atggtttaaa aattaaaatt ggcatacaaa gttcaaatga tgatgcgcaa taagctacaa
     1321 agtttgattt tttaattgaa cgttatatgt ttttaaaaga agaaagagct tcgaattaat
     1381 atcttaatgg aggaagatta gtaaatggct agaacgtata atgtgattaa atatgtcatt
     1441 aagaaaaata aggatgatct taaaatacat ggattaagat tatatgaatt taatataaaa
     1501 gatgaaaaag ctataaatat tagccaaccc atttttgtga acagagcaac attgattgag
     1561 aaaatacaaa atggtgaagc atttacgcac gcctttagag taaatcacaa tacttattat
     1621 gcaggccaat taatttcttt atcattgaat aaaaatggtt atattaataa caactttgag
     1681 gcctcacgag atattattag aaacattgaa gtaagtattt tagactatga ataaaggtaa
     1741 tactgataga atacattaca att
//
LOCUS       DAKTLH010000262         1727 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.269, whole genome shotgun sequence.
ACCESSION   DAKTLH010000262 DAKTLH010000000
VERSION     DAKTLH010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.269"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            261..>1727
                     /gene="eap"
                     /locus_tag="O0394_002799"
                     /gene_synonym="map"
     CDS             261..>1727
                     /gene="eap"
                     /locus_tag="O0394_002799"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
                     /protein_id="HCY0500808.1"
                     /translation="MKFKSLITTTLALGVLASTGANFNNNEASAAAKPLDKSSSSLHH
                     GYSKVHVPYAITVNGTSQNILSSLTFNKNQNISYKDLEDRVKSVLKSDRGISDIDLRL
                     SKQAKYTVYFKNGTKKVIDLKAGIYTADLINTSEIKAININVDTKKQVEDKKKDKANY
                     QVPYTITVNGTSQNILSNLTFNKNQNISYKDLEDKVKSVLESNRGITDVDLRLSKQAK
                     YTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSIN
                     LNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKN
                     GGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSD
                     LKFTGDPRVGYKDISKKVKSVLKHDRGIGERELKYAKKATYTVHFKNGTKKVININSN
                     ISQLNLLYVQDIKKIDIDVKTGTKAKADSYVPYTIAVNG"
BASE COUNT          751 a          212 c          261 g          503 t
ORIGIN      
        1 ataatcgaca aatctaatcg gaattaagaa atgaatgttg ttatgacatt taagtttgaa
       61 gcttggtata aacaaaacca aacaacattt taggtattgt aaattaatta gttaatcaaa
      121 attaaaagaa cttttaacag tgttaaaaaa ttattgttaa agataattta ttattaatat
      181 tcagttaatt caaaaaatag agaaagtctg gctataatta agttgcaaat acgaattaca
      241 caaaaaagga gagataattt atgaaattta agtcattgat tacaacaact ttagcattag
      301 gtgttttagc atcaacaggt gcaaacttta ataataatga agcgtctgcc gcagctaagc
      361 cattagataa atcatcaagt tcgttacacc atggatattc taaagtccat gttccatatg
      421 caatcactgt gaacggtaca agccaaaata ttttatcaag cttaacattt aataagaatc
      481 aaaatattag ttataaagat ttagaggata gagttaaatc agttttaaaa tcagacagag
      541 gtattagtga tatagattta agactatcga agcaagcgaa atatactgtt tactttaaaa
      601 atggaacaaa gaaagttatc gatttgaaag caggtattta cacagctgat ttaattaata
      661 caagtgaaat taaagctatt aatattaacg tagatactaa aaagcaagtt gaagataaaa
      721 agaaagataa agcaaattac caagttccat acacaatcac tgtgaacggt acaagccaaa
      781 atattttatc aaacttaaca tttaataaga atcaaaatat tagttacaaa gatttagagg
      841 ataaagttaa atcagtttta gaatcaaata gaggtattac cgatgttgat ttaagattat
      901 cgaagcaagc gaaatataca gttaatttta aaaatggaac gaagaaagtt atcgatttga
      961 aatcaggtat ttacacagcg aatttaataa attcaagtga tattaaaagt atcaatatta
     1021 acgtagatac aaaaaaacat atcgaaaata aagctaaaag aaactatcaa gttccatatt
     1081 caattaattt aaatggtaca tctacaaaca ttttatcgaa tctttcattt tcaaataaac
     1141 cttggacaaa ttacaaaaat ttaactagtc aaataaaatc agtactgaag catgatagag
     1201 gtattagtga acaagattta aaatatgcta agaaagctta ttatactgtt tattttaaaa
     1261 atggtggtaa aagaatctta cagttgaatt caaaaaatta cacagcaaac ttagttcatg
     1321 cgaaagatgt taagagaatt gaaattactg ttaaaacagg aactaaagcg aaagcagaca
     1381 gatatgtacc atacacaatt gcagtaaatg gcacatcaac accaatttta tcagatttaa
     1441 aatttacagg tgacccacgt gtaggctaca aagatatctc taaaaaagtt aaatcagtat
     1501 tgaagcatga tagaggtatc ggggaacgtg aattaaaata tgcaaaaaaa gcaacttaca
     1561 cagtacattt taaaaatgga acgaaaaaag tgattaacat aaattcaaat attagccaac
     1621 tgaatctgct ttatgttcaa gatattaaaa agatagatat tgatgttaaa acaggaacta
     1681 aagcgaaagc ggatagctat gtaccatata caattgcagt aaatggc
//
LOCUS       DAKTLH010000263         1680 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.270, whole genome shotgun sequence.
ACCESSION   DAKTLH010000263 DAKTLH010000000
VERSION     DAKTLH010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.270"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            362..763
                     /locus_tag="O0394_002800"
     CDS             362..763
                     /locus_tag="O0394_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HCY0500809.1"
                     /translation="MKKNITKTIIASTVIAAGLLTQTNDAKAFFSYEWKGLEIAKNLA
                     DQAKKDDERIDKLMKESDKNLTPYKAETVNDLYLIVKKLSQGDVKKAVVRIKDGGPRD
                     YYTFDLTRPLEENRKNIKVVKNGEIDSITWY"
     gene            723..815
                     /locus_tag="O0394_002801"
     CDS             723..815
                     /locus_tag="O0394_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500810.1"
                     /translation="MLKTVKSTRLLGIKKHTELNSCTPNVRKQC"
     gene            complement(1132..1461)
                     /gene="ecb"
                     /locus_tag="O0394_002802"
     CDS             complement(1132..1461)
                     /gene="ecb"
                     /locus_tag="O0394_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Ecb"
                     /protein_id="HCY0500811.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQ
                     TNFKKQVNKKVVDAQKAVNFFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLV
                     LKYNTLK"
BASE COUNT          606 a          245 c          214 g          615 t
ORIGIN      
        1 ttgttttata aaaaatatat atcaaaacag ctatatgtat aacaatttgg agtgtttgtc
       61 tatccaactt agcaaacaaa atgtagagaa atgtaaatat cgttttagca ataaaaatta
      121 ttcaatcata gttgagtgac gtttactact attattatac tatcaacaaa ttcaataata
      181 cctcaaataa aaataatacc atcaaattca tcattattct ttttcgattg cattaaacct
      241 tattaatttg cacttaattt taattaatga agtgttaact ggtaaatgta tgaaaccgtt
      301 tgtaaaatag cagtgtactc gcattagtaa gtacaaattt attttacagg gagaacttat
      361 tatgaaaaaa aatatcacaa aaactattat cgcatctaca gtaatcgctg caggtctttt
      421 aactcaaact aatgatgcta aagctttctt tagttatgaa tggaaaggtt tagaaatcgc
      481 aaaaaattta gcagatcaag ctaaaaagga cgatgaacgt attgataagt taatgaaaga
      541 gtctgataaa aatctaactc cttacaaagc tgaaactgtt aatgatctgt accttattgt
      601 taaaaaatta agccaaggtg atgtgaagaa agcagttgtc agaattaaag atggtggtcc
      661 tagagattac tatacttttg acttaactcg tcctttagaa gagaacagaa aaaatattaa
      721 agttgttaaa aacggtgaaa tcgactcgat tacttggtat taaaaaacat actgaattaa
      781 atagttgtac gccaaacgtt agaaaacaat gctaacgtat tggcgtgctt ttttatttta
      841 agtaacttcc aatttattta gcatctttac gactgtttaa taaagcacgt atgattaaca
      901 cggttgccac tatatccgtt acaattttta tgattgttaa tacattcgat ttccttttca
      961 caatagccac ctcgcttgtt caaaacataa acaactattg cattcacttt taagtaatta
     1021 tttatattat ttatcccaat aagctcacca ttcaaataaa cacaatatta taaaataatt
     1081 atacctttgg atatagcaaa aagccacact ctatagcatg gcttctatca attattttaa
     1141 agtattatat tttaaaacta gatcgatttg tctttgtaat tttttctttt cataactgtg
     1201 ttggaaatga attaaattaa cagctctttg tgctttacgg tgtgttgcaa cagttcttgt
     1261 acgtttgaaa aagtttacag ctttttgtgc atccacaact tttttattta cttgtttttt
     1321 aaagtttgtt tgatactgat catatttttt agcagcttca acgtttttag tttgtgcatg
     1381 agattcaccg gcaaacgttg ataccgttaa actaatagca gctatgctta aaattgattt
     1441 cccaataaaa ttctttttca taattaataa cacctatcct caaatattta ttttgtatat
     1501 atttataata atagacagat acaaaaaact acattaacta tttattaact aaacattaat
     1561 atttgtgtca ctacatttct gaatttcaag aattttgcta ctccatattt taaccatcaa
     1621 attattgata taatttatga ctttagaaat ccttttaaat atgtatacga ttcatgattt
//
LOCUS       DAKTLH010000264         1672 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.271, whole genome shotgun sequence.
ACCESSION   DAKTLH010000264 DAKTLH010000000
VERSION     DAKTLH010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.271"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..206)
                     /locus_tag="O0394_002803"
     CDS             complement(<1..206)
                     /locus_tag="O0394_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500812.1"
                     /translation="MNHDNTIQSNWRTVASCLASQNYISIVKGLVHHFTAIDDEEILD
                     KIYEEFINDDSITTVLNNDLQTII"
     gene            complement(220..723)
                     /locus_tag="O0394_002804"
     CDS             complement(220..723)
                     /locus_tag="O0394_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCY0500813.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKIWDDKKPACTVITMYLHL
                     DGVLSLDLTTVLILNQLANSEQYGAVYLVNLFSNIKTPENLKHIKEPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEVMHPLNPKA
                     RQKWTLK"
     gene            complement(739..1050)
                     /locus_tag="O0394_002805"
     CDS             complement(739..1050)
                     /locus_tag="O0394_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500814.1"
                     /translation="MKINRYITRGISEQLSLDLQILLWNMVKDRDNQLNTDYLHIFKL
                     QEDDNMLSITHEQEQPAYKLEYHYTNYIKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            complement(1137..1487)
                     /locus_tag="O0394_002806"
     CDS             complement(1137..1487)
                     /locus_tag="O0394_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500815.1"
                     /translation="MKTTTQELKQYMTRLFQLSNNETWECETLEEAAENILPKRFIND
                     SPLAHLILETYTYYNNELHELSIYPFLMYSNNQLISIGYLDHFDMDFLYLTDTKNTII
                     DERHLLKEGGNNHE"
BASE COUNT          472 a          270 c          295 g          635 t
ORIGIN      
        1 ttaataattg tttgtaaatc attgttaagt accgttgtaa tagagtcatc atttataaac
       61 tcttcataga ttttatcaag tatttcctcg tcatcgatgg ctgtgaaatg atgcaccaga
      121 ccttttacta ttgaaatgta attttgtgat gctaaacaac tagcaaccgt tcgccaattg
      181 gattgtatag tgttgtcatg gttcatagat aatactcctt tactttagtg tccatttttg
      241 acgtgcttta gggttgagtg gatgcataac ttcgttcgtt actggattta tgagcttttt
      301 tactttcttt ttatgaggtt ttaacatttc catcacttgt tcgacacggt cgataacaac
      361 tggtcgcttc gcataggcac cataagcaag aatgactgtg tcactttcac taattgcttt
      421 cattaagtgt atatctgtgt gctcatcata aggctctttg atatgtttaa ggttctctgg
      481 tgttttaata ttagagaaaa gattaacgag ataaacagcg ccatattgct cagaattagc
      541 taattgattg aggatgagaa ccgtagtaag atcgagtgat aatacaccat ctaaatgaag
      601 atacatcgtt atcacagtac aagccggttt cttgtcatcc catatctttt tgagtaagta
      661 gcgatgttgt tcatcatcgc taaatatcgc ttctgtgtgt atcgtacttt tgattgtatt
      721 catatatcgt cacttccttt agtattcttc tggcaaaagc atcacataat aaaaagcgtc
      781 tacatcatct tctcggatga cgtagacttt cttaggtaat gcattttgat tttttatata
      841 gtttgtatag tgatattcca atttgtatgc gggttgttct tgttcatgtg taattgacaa
      901 catattatca tcttcttgta gtttgaaaat gtgtaggtaa tctgtattaa gttgattgtc
      961 tcgatctttt accatgttcc aaagtaagat ttgaaggtct agagatagtt gttcactaat
     1021 gcctcttgtg atgtatcgat tgattttcat actattttcc tccattttgc ttttctttca
     1081 tgatgtcaat cacttcgtta atgactgtaa cagatatttg tgccactttg atccaattat
     1141 tcatggttat tccctccttc ttttagtaaa tgacgttcat cgataatcgt atttttagta
     1201 tctgtgaggt atagaaagtc catatcaaaa tgatccagat aaccaatact gatgagttgg
     1261 ttattagaat acattagaaa tggatagata cttagttcat gtagctcatt attatagtag
     1321 gtataagttt caagtataag atgtgcaagt ggggaatcat taataaaacg tttgggtaga
     1381 atattttctg ccgcttcctc cagtgtttca cattcccatg tttcattgtt agataattgg
     1441 aataagcgag tcatatattg tttgagttct tgagtagttg ttttcatatc attgcctccc
     1501 tagatagtgt gatagtgatg tacttcatat acatcattga gataatatat atttgatttg
     1561 tcatttatta ctgatcccgt tgacaatatg agaaaattcc atataaaaaa ccgctacaaa
     1621 ccttggtatg acaaggaaat cccgaaattc cgcctatttt gacgaacaat ca
//
LOCUS       DAKTLH010000265         1659 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.272, whole genome shotgun sequence.
ACCESSION   DAKTLH010000265 DAKTLH010000000
VERSION     DAKTLH010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.272"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            218..901
                     /locus_tag="O0394_002807"
     CDS             218..901
                     /locus_tag="O0394_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCY0500816.1"
                     /translation="MTTKENIDTLRKPGAQALSLISLFLILFSCLTFFFGLDYERFPN
                     YLKITTIIELIIIVISLLQWIRFIDFEKESAQKYKKIYARFLVVINVLTTITVVFALC
                     NLYYFAAVQNHYDLFNYWLMGSISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTHF
                     GLLLTALSSFIYIEKIIEYILVPNVVESKFIIIVSMLVIAGAQFVAFQFIMQYSRFYI
                     FELNTEDDD"
     gene            1048..1659
                     /locus_tag="O0394_002808"
     CDS             1048..1659
                     /locus_tag="O0394_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCY0500817.1"
                     /translation="MEFLLLIVVAGLYYIIYLTAVMYSEKIVVLPIIIYAIVFVIIGI
                     TYIFIGDSYDQLTNFNVILYMGSLFYAWMAIRNLWNRPLLLKYKNITDSSSGIVNKSE
                     YNSVESLRINIEIAKYKGIISLIVAIVLTVLMTLKSTPQITAETRDLSISFFILSLFI
                     IIIFAVWDLFIRVRKGAFAFVVIRPILFSCWLFILNMILSRLL"
BASE COUNT          583 a          177 c          263 g          636 t
ORIGIN      
        1 agaagaataa actatcttaa tgtaagacta aacaataaag ctttgtttag tctttttagc
       61 gtttaagtaa aaagcaatag ataccgtaaa gttgatgctc atcaaataat aatataaaga
      121 taattttagg tttttaaact tttaatcgaa aagaatctaa gtttatagct ttaaatacgt
      181 aatatgctat ttaaaaaata atgtatagga gatagatatg actactaaag aaaatattga
      241 tactcttcga aagccaggcg cacaagcctt aagtttaata tcattgtttt tgatactttt
      301 ttcatgtttg actttctttt ttggtttaga ttatgaaagg tttccaaatt atttaaagat
      361 aacgacaatt atagaattaa taattattgt aattagttta cttcaatgga ttagatttat
      421 agatttcgaa aaagaaagtg cacagaaata taaaaaaata tatgctcgat ttttagttgt
      481 tataaatgtg ttaactacta tcactgtagt atttgcactg tgtaatcttt attattttgc
      541 agctgtacaa aaccattatg atttatttaa ttattggttg atgggttcga tttcaatcat
      601 aattagctat ttattattag taattggcgg aatgttcacg ttattaaaat tacctaaagt
      661 aacaaaacgt tggggtggta aaactaaaac acatttcggt ttattattaa ctgcgttgag
      721 ctcatttata tatattgaaa aaattatcga atatatattg gttcctaatg tcgtagaatc
      781 taagtttata attattgtga gcatgttggt tatcgctgga gcacaatttg tggcatttca
      841 atttattatg caatacagta gattctatat ttttgaatta aacactgaag atgatgacta
      901 aacaatttta taaattctgt gaagtatact tatatataag ataatcaact tcaacgtata
      961 tcaccatatt attttatact caatgatgtg aatggattta agtggattga tattaaagta
     1021 aaagttatgg aatattgagg tgtgaatatg gagttcttat tattaattgt cgtagccgga
     1081 ctgtattata ttatatattt aactgctgtg atgtattctg aaaaaatagt agtattgcct
     1141 ataatcatct atgccattgt gtttgtaata attggtatca cttatatctt tataggcgac
     1201 agctatgatc aattaacaaa tttcaatgtg attttgtata tggggagttt gttttatgca
     1261 tggatggcta ttagaaatct ttggaacaga ccactattac taaaatataa gaacattaca
     1321 gatagttcaa gtggaatagt taataaatct gaatacaatt cagttgaaag cttacgtata
     1381 aatattgaaa tagctaagta taaagggatt atttctttga tagtagctat agtactaacg
     1441 gtattaatga cattaaagtc aacacctcaa atcactgcag aaacacgtga cttaagtatc
     1501 tcatttttca tactcagctt atttatcatt attatatttg cagtttggga tttgtttatt
     1561 agagttagaa aaggagcgtt tgcttttgtt gtaataaggc caatattatt tagttgttgg
     1621 ttatttattt tgaatatgat tttatcaaga ttattataa
//
LOCUS       DAKTLH010000266         1655 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.273, whole genome shotgun sequence.
ACCESSION   DAKTLH010000266 DAKTLH010000000
VERSION     DAKTLH010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.273"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(38..1606)
                     /locus_tag="O0394_002809"
     CDS             complement(38..1606)
                     /locus_tag="O0394_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCY0500818.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGGIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
BASE COUNT          598 a          348 c          236 g          473 t
ORIGIN      
        1 tcaaatgatc actacatata ctataaacaa aagtagatta ctttttggca cgtgcttgtt
       61 ttaaacgttc tggatacgtc ttcaaccaaa agacagcatt aggtaattta ttcggatcag
      121 gttgataaac tgcattataa ccgttgccta aacgtttctt ttgaatttca taatctttta
      181 aagcatttac ggcaggctta tgtaaaatcc aaatcgcaat gatatttaac caagccatta
      241 gacctacacc taaatcaccg aatgcccatg ctacatcagc tgttttaact gcaccgtaaa
      301 atgtagcgaa caaaataatc acacgagcaa tattaataaa tatcgatgaa acttgattat
      361 tttgattacg cgttaaataa gcaacatttg tttctgtaat gtagtagtac gacaaaattg
      421 tagtaaatgc aaagaagaat aaagcaaatg caataaagta cgaacctacg ccagagaaag
      481 taggatcaaa ttgataacca ctgccatgga acgctttatc aatgccggct tgtgcataca
      541 tcgcagtacc tgaataatct ttacctgttg cattttcaac ataaataccg ccatctttaa
      601 ttaaatgcgg tgtgccattc gcattaaccg taccatcagt cacattatat gtaccagaaa
      661 taagtataat cagagcagtt gcagtacata caaataatgt atcaatataa actgaaaatg
      721 cttgtactag accttgttta cttggatgtg atacttctgc tgccgctgct gcgtgtggac
      781 ctgtaccttg accagcctca tttgaatata atccacgttt aacaccaatc tctatcattg
      841 cgccaacgat accaccaaaa gcagattgta atccaaatgc tgatttgaaa attaatgcaa
      901 ataacgctgg cacttcttgt atattgatac aaatgataat gacagccatc agtatgtaaa
      961 taattgccat aaatggtaca acggctgttg caacattggc aatactacgt acaccaccaa
     1021 aaataattaa tcccaaaata actacaacaa taccacccat taaccattgt ggaacatgaa
     1081 tcgcattatg catagaactt gctatagcgt tagattgcac accaggaagc aatagaccta
     1141 ctgagataat cgtaacaata gcaaagataa ttccgtaaat tttaccaaat ttaccaccaa
     1201 taccatattc aatataatac gctggtccac cacggtattc attattttca actctcttga
     1261 atatttgacc aagtgtagat tcaataaaag cactacttgc acctaaaaac gcagtaatcc
     1321 acatccaaaa tactgcacca ggtcctccta taaatattgc agtagataca ccgacaatat
     1381 tacctgtacc aaccctgcct gctaaagaca tggctatcgc ttgaaaactt gaaataccat
     1441 taggagactt ctctccttga aacattaatc gaatcatttc tttaaaatgt ctaacttgaa
     1501 aaaatcgcat acgcaatgta aatagcacac cagtaattag caaaccataa actaatggct
     1561 tactccaaac aacctcattt aaaaaggtta ctaatttttc aatcataaga ttccccctat
     1621 tgtttaaaca taaaaatatt atacgattaa gcacg
//
LOCUS       DAKTLH010000267         1609 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.274, whole genome shotgun sequence.
ACCESSION   DAKTLH010000267 DAKTLH010000000
VERSION     DAKTLH010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.274"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            266..772
                     /locus_tag="O0394_002810"
     CDS             266..772
                     /locus_tag="O0394_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500819.1"
                     /translation="MIIKILTILLLLCILSYLVTNRKKPFLFLKTLFMGVVFIFIGYI
                     SLAISAVIIYGIIQFITIDFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVV
                     LEHLIQWFLVYFAIYQAVNEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIF
                     NYRMRSYK"
     gene            1031..1528
                     /gene="efb"
                     /locus_tag="O0394_002811"
     CDS             1031..1528
                     /gene="efb"
                     /locus_tag="O0394_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HCY0500820.1"
                     /translation="MKNKLIAKSLLTLAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTD
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLK
                     QGLVK"
BASE COUNT          610 a          183 c          248 g          568 t
ORIGIN      
        1 ttataaagaa ataagagata tattctaatt atggttgata tgttatgatt aatatataag
       61 ttaaaaatta tactatttaa ttgaattatg cattttcgaa gtaaacataa ttcaacactt
      121 acatttttaa atagataaaa tagtgtaaaa tcacgtgcca agattactat agagtaggca
      181 ttgagtatta ttggcaatca cttaagtgat tgttattttt cgtggtcaat aataaagata
      241 taaataaata tattgaggtc aaacgatgat aattaaaatt ttaacaattc tattactact
      301 ttgtatattg agctatttgg ttacaaatag aaagaagcct tttctgttct taaagacact
      361 ctttatgggt gtggtattta tctttatagg atatatttca ctggcaatat ctgccgtaat
      421 tatttatggt attattcaat ttatcacaat tgattttggt agttttttct taatgggtat
      481 tatattgatc ttgatttcaa gtatattcca attatttata gttagattac tttttagaaa
      541 aaagaatgtc gatttgacag aggttgtcgt tttagagcat ttaattcaat ggttcttagt
      601 ttactttgcg atctatcaag cagtaaatga aaaaatggac attaatgata ttaatatcga
      661 caatttccaa tctgtctttt ttgacgtgtc taatttgaat ttagtaattc taccaacgtt
      721 aatcattagc tgggtcacaa tatttaacta tagaatgaga agttacaaat aaaatctatg
      781 agattatacc ttcagacacc aacattcaaa tggtgtcttt tttgttgtgt ggttttattt
      841 ttgaaattcg aaaaagtaga ggcatgaatt ttttgactag tgtataagtg ctgatgagtc
      901 acaagataga tagctatatt ttgtctatat tataaagtgt ttatagttaa ttaataatta
      961 gttaatttca aaagttgtat aaataggata acttaataaa tgtaagataa taatttggag
     1021 gataattaac atgaaaaata aattgatagc aaaatcttta ttaacattag cggcaatagg
     1081 tattactaca actacaattg cgtcaacagc agatgcgagc gaaggatacg gtccaagaga
     1141 aaagaaacca gtgagtatta atcacaatat cgtagagtac aatgatggta cttttaaata
     1201 tcaatctaga ccaaaattta actcaacacc taaatatatt aaattcaaac atgactataa
     1261 tattttagaa tttaacgatg gtacattcga atatggtgca cgtccacaat ttaataaacc
     1321 agcagcgaaa actgatgcaa ctattaaaaa agaacaaaaa ttgattcaag ctcaaaatct
     1381 tgtgagagaa tttgaaaaaa cacatactgt cagtgcacac agaaaagcac aaaaggcagt
     1441 caacttagtt tcgtttgaat acaaagtgaa gaaaatggtc ttacaagagc gaattgataa
     1501 tgtattaaaa caaggattag ttaaataaaa cttcaatcgt tgctgttatc tggaaataat
     1561 taattaaatg ttatgttaat ttttgttaat gaaaaaagta atctattta
//
LOCUS       DAKTLH010000268         1581 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.275, whole genome shotgun sequence.
ACCESSION   DAKTLH010000268 DAKTLH010000000
VERSION     DAKTLH010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.275"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            86..586
                     /locus_tag="O0394_002812"
                     /pseudo
     CDS             86..586
                     /locus_tag="O0394_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532440.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(646..1143)
                     /locus_tag="O0394_002813"
     CDS             complement(646..1143)
                     /locus_tag="O0394_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0500821.1"
                     /translation="MIINLETQQIHFDLEHEIPYFQAPEKNRIRLDIDVLNKKQISNI
                     INVIFNNQSKTKCTFLSEYLYPVKFREKTRIGRFFNITNWYEEIHSTDEKYVIATIKN
                     IKNKDLIDYSLYIKKGLVEPFIVFYNQENIFYISNAVIDIISVSKDTLDYIKSEFNDH
                     CASPY"
     gene            complement(1145..1231)
                     /locus_tag="O0394_002814"
                     /pseudo
     CDS             complement(1145..1231)
                     /locus_tag="O0394_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1246..1572
                     /locus_tag="O0394_002815"
                     /pseudo
     CDS             1246..1572
                     /locus_tag="O0394_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          534 a          247 c          241 g          559 t
ORIGIN      
        1 cctattattt aaaacgtcgt gaagaaaaag gtaaataaat aagccttatg tgctaaactc
       61 aatatgtatt tcctaccaaa gaaaacataa ggagtttgga catcttgaag gagatactat
      121 tgttggtcgt cgtcatggta gtgcaatcat tactttggta gaaagagtat ccaagatgat
      181 tatcacctta cgacccttag gtcgtaaggc aactgatata gaaaacgctc tacactcaat
      241 gttttcatct ttaccttctc atatatttaa atcaattact tttgattgtg gtaaggagtt
      301 ctcaaactgg aaaacattat gtaactgtca cgatatatct atattttttg ctgatccagg
      361 cacgccatca cagcgaggtt taaatgaaca ttctaacgga atacttagac gcagtggatt
      421 agataaagaa ctagatttca atttagtaac agacgatcat atcatcagtg ttgctcaaaa
      481 aatcaatcat cgtcctagaa aatctttagg ttatagaaca ccattggaag tatttatgag
      541 tttcatcgaa gatgataaac ttgtccagct taatttgaca atctaaagat ttataaaaaa
      601 gccgttggtc tcttcgaaac tacggctttt ctctgtgcta taaaatcagt atggcgaagc
      661 acagtgatca ttgaattctg actttatata atcaagagta tctttggata cactgatgat
      721 atctataaca gcattgctaa tgtagaatat attttcttga ttatagaata caataaatgg
      781 ctccactaaa ccttttttaa tatataaaga ataatcaatt aagtctttgt tttttatatt
      841 tttaatagtt gcaataacat acttctcatc ggtagaatgg atttcttcgt accagttagt
      901 aatattaaaa aaacgaccaa ttcttgtttt ttctctaaat ttaacaggat ataagtattc
      961 agacaaaaat gtacatttcg tctttgattg gttattaaat ataacattaa taatattaga
     1021 aatttgcttt ttattgagaa catcaatatc taagcgtatc ctatttttct caggtgcttg
     1081 aaaataggga atttcatgtt ctaaatcaaa atgtatttgt tgtgtttcaa ggttaataat
     1141 cattttaatc tccaattctt tttaggttag catcgtatgt accacttctt gttttcttgc
     1201 tacctaaatt cttaatactg cttgcacctt ttcattttat cttctcatat atttaaatca
     1261 attacttttg attgtggtaa ggagttctca aactggaaaa caatatgtaa ctgtcacgat
     1321 atatctatat tttttgctga tccaggcacg ccatcacagc gaggtttaaa tgaacattct
     1381 aacggaataa ttagacgcag tggattagat aaagaactag atttcaattt agtaacggat
     1441 gatcatatca tcagtgttgc tcaaaaaatc aatcatcatc ctagaaaatc tttaggttat
     1501 agaacaccat tggaagtatt tatgagtttc atcgaagatg ataaacttgt ccagcttaat
     1561 ttgacaatct aaagatttat a
//
LOCUS       DAKTLH010000269         1578 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.276, whole genome shotgun sequence.
ACCESSION   DAKTLH010000269 DAKTLH010000000
VERSION     DAKTLH010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.276"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            8..820
                     /gene="lpl8"
                     /locus_tag="O0394_002816"
     CDS             8..820
                     /gene="lpl8"
                     /locus_tag="O0394_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HCY0500822.1"
                     /translation="MGYIKRMALYMSVFLLIIFIVGCRNMKDEQKKEEQTNKTDSKEE
                     QIKKSFEKTLDMYPIKNLEELYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKQGGLSNE
                     GMVLYLDRNTRTAKGHYFVKTFYNKGKFPDRKNYKVEMKNNKIILLDKVEDTNLKKRI
                     ENFKFFGQYANLKELKNYNNGDVSINENVPSYDAKFKMSNKDENVKQLRSRYNIPTDK
                     APVLKMHIDGNLKGSSVGYKKLEIDFSKGGKSDLSVIDSLNFQPAKVDEDDE"
     gene            839..>1578
                     /gene="lpl9"
                     /locus_tag="O0394_002817"
     CDS             839..>1578
                     /gene="lpl9"
                     /locus_tag="O0394_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl9"
                     /protein_id="HCY0500823.1"
                     /translation="MKSIKRIGLCISLLILIIFVTSCDGDNKIIGDSKEEQIKKSFAK
                     TLDIYPIKNLEDLYDKEGYRDGEFKKDDKGTWLIRSEMKIQLKGENLESRGAVLEINR
                     NTRTAKGHYIVREVVEDSDGMTHNHTKRYPVKMENNKMIPLKPIDDEKVKKEIEEFNF
                     FVQYGNFKELENYKEDEVSYNPEVPIYSAKYQLKNSDYNVEQLRKRYNIPTQKAPKLL
                     LKGSGNLKGSSVGYKNIEFTFIENKEEN"
BASE COUNT          676 a          169 c          307 g          426 t
ORIGIN      
        1 tgaaatgatg gggtatataa aaagaatggc tttatacatg agtgtatttc ttttaatcat
       61 ttttattgtt ggatgtcgaa atatgaaaga tgaacagaaa aaagaagaac aaacaaataa
      121 aacagattca aaagaagaac aaatcaaaaa gagttttgag aaaacattag atatgtatcc
      181 aattaagaat ctcgaggagt tatacgacaa agaaggatac cgagatggcg aatttaaaaa
      241 gggtgataaa gggatgtgga cgatatatac agatttcgcc aaaagtaata aacaaggtgg
      301 attgagtaat gaaggtatgg tcttatactt agatagaaat acacggactg caaagggaca
      361 ttattttgtt aagacattct ataataaggg caaattccca gatagaaaaa attataaagt
      421 tgaaatgaaa aataataaaa ttatcttatt agataaagta gaagatacaa atctaaaaaa
      481 gagaatagaa aactttaaat tttttggaca atatgcaaac cttaaagaat tgaaaaacta
      541 caacaatggt gatgtctcaa ttaatgagaa tgttccaagt tatgacgcaa aatttaaaat
      601 gagcaataaa gatgaaaatg ttaagcaatt aagaagtcgt tataatattc ctactgataa
      661 agcaccggta ttaaaaatgc atattgatgg taatttgaaa ggaagttctg tgggttataa
      721 aaagttggaa attgactttt caaaaggtgg aaaaagcgat ttgtcagtaa tagattcttt
      781 gaatttccag ccggcgaagg tagatgaaga tgatgaatga tgaggatggt gtgtaacaat
      841 gaagtctata aaaaggattg gattgtgcat tagtttgttg attttaatca tctttgttac
      901 atcttgtgat ggtgataata agatcattgg agattcaaaa gaagaacaaa tcaaaaagag
      961 ctttgcgaaa acgttagata tctaccctat taagaatctc gaggatttat acgacaaaga
     1021 aggatatcga gatggcgaat ttaaaaaaga tgataaaggt acttggctaa ttagatctga
     1081 aatgaaaatc caattaaaag gagaaaatct ggaatctaga ggagcagttt tagaaattaa
     1141 cagaaatact agaacggcta aagggcatta tattgttaga gaagttgttg aagatagcga
     1201 tggaatgaca cacaatcata caaaaagata tcctgtgaaa atggaaaata ataaaatgat
     1261 tccattaaaa ccaatcgatg acgaaaaagt aaaaaaagaa atcgaagaat ttaacttctt
     1321 tgtacaatac gggaatttca aagaattgga aaactataaa gaggacgaag tgtcatataa
     1381 cccagaagta ccaatttact ctgcaaaata tcaattgaaa aacagtgatt acaatgttga
     1441 acaattaagg aagcgatata atatcccgac gcaaaaagcg cccaaattat tattgaaagg
     1501 ctcaggtaat ttaaaaggtt catcagtcgg atataaaaat attgaattta cctttattga
     1561 aaacaaggaa gaaaatat
//
LOCUS       DAKTLH010000270         1499 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.279, whole genome shotgun sequence.
ACCESSION   DAKTLH010000270 DAKTLH010000000
VERSION     DAKTLH010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.279"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            88..1035
                     /locus_tag="O0394_002818"
     CDS             88..1035
                     /locus_tag="O0394_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCY0500824.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
BASE COUNT          578 a          171 c          291 g          459 t
ORIGIN      
        1 cagaagtata gtaaaaaaat tattttacta tataataaat agagaaaaca gacaaagtat
       61 gtaaggaata gtaaaggagt ggtgggattg aaacgttcgt ctaaaagtaa aatgagccta
      121 ccgaaaaaaa tatttttatg ggtattcggt attttagtaa tcttagcgat tgtagctgtt
      181 gtgtatgtag ctgctaaaat ttttattact ggtaataaga tacacaatcc gttagatcgc
      241 aatcattcag aattaagaga taaaaaagtg agtttaaacg atggtgatcc attcacaatc
      301 gctttatttg gtgtcgattc agatgctgat cgtaagaaaa agggtggcgg agagcgtagt
      361 gatagtatta tgattttatc tatcaaccct aaaacgaaga aaactgaaat agtaagtata
      421 ccgcgtgata caagagcgga aattgttggc cgtggtacta ctgaaaaaat tgcacatgcc
      481 tatgcatatg gtggaccaaa tatggctgtt aaatcacttg aaaaattaat gaatgtgcca
      541 attgatcatt atgcgacaat cgatatggat ggtttgcata atatgataga tagcatcggc
      601 ggtgttgatg tagtaagtaa tgatacattc actgttgatg gtgttcgttt tacaaaagga
      661 cagcagacac atgtaaatgg tgatcaagca ttgaagttta ttagaagtcg taaagaagaa
      721 ggtgctggtg gtgactttgg tcgacaacaa cgtcagcaaa ttgtgcttga agcgatggcc
      781 aataagattg caagcccatc atcgattaca cattttaata gtctaatgaa tgaaattcaa
      841 aataatgtta aaacagattt aacattaggt gatttgaata cgattagaag caattataaa
      901 gatgctaatg acacgattaa taaacatcaa ttgagtggtc aaggcggcat tcaaagtgac
      961 ggattgtact acttcattcc tagtgagcag tcaaaagctg aaagtactaa gttattaaaa
     1021 gataatctag agtagtaaac taagggaaaa taagttatca aaattgtaca caaattaatt
     1081 agatagaatg atctgtaaat agaataacga atagaactaa tagcccgtaa atagaaggtg
     1141 ataacacttt gtatttatgg gctattttat ttaatgcctg atttgatttt tataaattaa
     1201 tccattacaa atttaattta aagataaata ttaccttcta aatgatagta gatatttatc
     1261 aaacttgggt tattaatttt cagaaaaagg gtatttataa atagtataac gatttttagg
     1321 tgaggtgatt attaaatatt gtcattaata aggttgtgaa agatatggca aaaagcgtga
     1381 aacagataat tggaaaacta ataatgataa ttattttcaa ttaaacaaaa agatgattta
     1441 atttcgcgca aaatatttca aataaaaatg attgtaaaaa ggcaaaatac aaaatttta
//
LOCUS       DAKTLH010000271         1477 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.280, whole genome shotgun sequence.
ACCESSION   DAKTLH010000271 DAKTLH010000000
VERSION     DAKTLH010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.280"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(305..>1477)
                     /locus_tag="O0394_002819"
     CDS             complement(305..>1477)
                     /locus_tag="O0394_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCY0500825.1"
                     /translation="FPGFEGEWEEKQLGDLTDRVIRKNKNLESKKPLTISGQLGLIDQ
                     TEYFSKSVSSKNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRYDSGVLSSLYICFS
                     IKSEMSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFFTILIKYPSLEEQQ
                     KIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEDYPDWENSKIE
                     KYLKERNERSDKGQMLSVTINSGIIKFSELDRKDNSSKDKSNYKVVRKNDIAYNSMRM
                     WQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIHKFKINSQGLTSDTWN
                     LKYKQLKNINIDIPVLEEQEKIGDFFKKMDILISKQKMKIEILEKEKQSFLQKMFL"
BASE COUNT          445 a          263 c          151 g          618 t
ORIGIN      
        1 caatggtcgt gcttcacatt aaacttactt taactaaaaa ttaatcatta ttaagtaatt
       61 taataaatag ttaactaata tcattcaatt cctatcgatc tactctcttt tatttacgaa
      121 taacacttta tctcaactta atctttattt aactacttat tctgtacaca atttcgacac
      181 aaaaaagaca ctgcgctttc acagtgccta aatttaatgt tcgtttttaa ttcactttac
      241 ttttacaact tttatgttaa attatacaaa ttttattctt atgcaatcta tgtatttatc
      301 aaagttataa gaacattttt tgtaaaaagg attgtttctc tttttctaat atttcaattt
      361 tcattttctg tttacttatc aatatatcca tttttttaaa gaaatcacct atcttttctt
      421 gttcctccaa tacaggtata tctatattta tattttttaa ttgtttatat tttaagttcc
      481 atgtatctga tgttaatcct tgtgaattaa ttttaaattt atgaatcatt ctatgtgttt
      541 taaacttata tccaataaat aatgagctag tattttgtgt tggataaagc acagtatatg
      601 cagggctaac aatcccatta taatttgatt taccactagc cccttgccac attctcatag
      661 aattatatgc aatatcattt ttcctaacta ctttataatt acttttatct ttacttgaat
      721 tatcttttct atccaattca ctaaatttta taatgccact atttatagtt actgaaagca
      781 tttgcccttt gtcagaacgt tcgtttctct cttttaaata tttttctatt ttgctatttt
      841 cccaatctgg ataatcttca ccattctcat ctttgaatcg cagttcctgt gagaaaattt
      901 tctgcatata gccttttttc tgttgttgaa gtaattcaag cttttgttct tctaattcaa
      961 tttgtcggtc gagtttgctg aagaacttgc ctattttttg ctgttcttct aaacttggat
     1021 atttaattag aatagtaaaa aaatcattca cagaaacatt taataatccg tgatttcttg
     1081 caccctcaac tgcaattcca gaaacttctc tataccagtg tgtcgaatca aaatatgctt
     1141 ccatgaagtc tttagacatt tcacttttaa tagaaaaaca aatatacaaa gaggacaata
     1201 caccactatc atatctagtt aatcttttaa tagcccctaa tgggtatcca ttagaataac
     1261 ttttgttata cgcgaattct ccattcttta ttagtgtata attttctaga tttttcgacg
     1321 aaactgattt actaaaatat tctgtttgat caattaaacc taactgtccg gatattgtta
     1381 aaggcttttt cgattctaag tttttatttt tcctaattac tctatctgta agatccccta
     1441 actgcttctc ttcccattcg ccttcaaacc ctgggaa
//
LOCUS       DAKTLH010000272         1432 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.281, whole genome shotgun sequence.
ACCESSION   DAKTLH010000272 DAKTLH010000000
VERSION     DAKTLH010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.281"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..191)
                     /locus_tag="O0394_002820"
     CDS             complement(<1..191)
                     /locus_tag="O0394_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500826.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTDWNIQTHIYKFNQIV
                     TNELNKFKGFETSYIINEN"
     gene            240..977
                     /gene="sdpB"
                     /locus_tag="O0394_002821"
     CDS             240..977
                     /gene="sdpB"
                     /locus_tag="O0394_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HCY0500827.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
     gene            complement(1068..1427)
                     /locus_tag="O0394_002822"
                     /pseudo
     CDS             complement(1068..1427)
                     /locus_tag="O0394_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          469 a          182 c          235 g          546 t
ORIGIN      
        1 tgattttcgt ttattatata tgatgtttca aagcctttga acttattcaa ttcattagta
       61 actatttggt taaatttata aatatgcgtt tgaatgttcc aatctgtcat taaagtatta
      121 tatttaatcg tatcaatgat ttgaatcaaa ttagtaattt gttgtctcat atacttagtt
      181 tcgtttatca tgtagaagta cctccaaaaa tcaattatta aacaggaaag gatataacag
      241 tggaaaatga aaaaaagaaa tacacgttta aagatatagc atggagagat ttgcttataa
      301 ttccaattat aggtgttata ttttatgcac tcacaattgc agcttacata ggaccaattt
      361 tgtttggtta ttatgaaatc gatagtaatc tgatatttgt agcgactatc acacaagctt
      421 tagcatatac gattgggatt ggcgcatttt atttatttca tttaaaagtg atgccatcaa
      481 ggcttcgagc tggttttgcg tatatcaaaa aatattggct tagattgtca ataacgtata
      541 ttattgctct catcttaatt tatacatatg aatatatgac acagtttcta cctaaacatt
      601 tgcaatatag tgaaacagcc aatgaacttg agctaaataa aatgtttgaa gtgcctgcat
      661 ttttacctgt agcattctta ttaattgtga ttgtaggtcc aatagttgaa gaaatcgtat
      721 ttagacatat tttaattgga gaattaggta agaagtttaa ttttatagct atgagtattg
      781 tttcggtgtt cttatttgca tttattcatg tgactgatgc aaagtcgccg tttgagtttg
      841 gtccatatct catactatcc atcatattag ttttcactta tttaaaatct ggaagaaacc
      901 taggttcaac aattgcatta catatagcaa ataattttgt ttctttcgtt atatcagtaa
      961 taagtattta tgcgtagata aagaggtcgg gacattaagt ttttaggcaa tgaaaaaagc
     1021 tgatttctat taattatttg atagaaatca gcttttttga tatgcatttt attatgaaac
     1081 tgaataaata aagattcaat tagcgcatca gtattaggat tcactctaaa aagattaata
     1141 gttttataag aaggtgtttg atcttgagct aaccacatca ttcgaatact gttatgaagt
     1201 aatttttcta ttctacggcc agaaaattca gattgagtat atgaatatga ggtgattttt
     1261 aacatcattt ttagatgata ggatgttgcg ccacgatgat gtctgaattc atcgaattcg
     1321 ctatcagcta tctttttaac aatttcatta acatatcgcg aaatatcatt ttgaggaatt
     1381 ctaacagcag tttctattgg tagtgtaagt tgggcaaagt gttttatttt tt
//
LOCUS       DAKTLH010000273         1409 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.282, whole genome shotgun sequence.
ACCESSION   DAKTLH010000273 DAKTLH010000000
VERSION     DAKTLH010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.282"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            47..601
                     /locus_tag="O0394_002823"
     CDS             47..601
                     /locus_tag="O0394_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCY0500828.1"
                     /translation="MNKKLLTKTLIASALVLTTVGSGFHSSSNYNGINNVAKAEQTTD
                     NELWKNVRDALKEANIIDKTDNENVKVTYKLANGGENSIEGTANLNELSNSNNKPINS
                     DSVKSIDLSRKNPNGDRFDANDAWKKLTDKLKEKQIVKDGDTVTIHSKDEKDPKISAK
                     VGEVYNDNKRLMLFKKDIDKITIN"
     gene            complement(836..>1409)
                     /locus_tag="O0394_002824"
     CDS             complement(836..>1409)
                     /locus_tag="O0394_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCY0500829.1"
                     /translation="ATFSSVMSNRKNKPLKHYINTHNGVPLWILVNYLTLGNVSKMYS
                     NLDDDLRLEVAKDYKRKLERDYKTRVQITPSDVDSILQQAHMFRNVCAHEERLYDYKI
                     DRAKSRANIFANYNKIYDKEYVPTMNGSYVFDLLISLCLFLNKHDYIKLMKNMDKLIS
                     NYSHSFYTITIDDLYTKMNFPDQTKILDML"
BASE COUNT          531 a          203 c          197 g          478 t
ORIGIN      
        1 aaattacttt gtaatcgatt gcaaataaat tataggagaa aataaaatga ataaaaaact
       61 attaacaaaa acattgatag caagtgcttt agttttaaca acagtaggtt caggttttca
      121 ttcttcttca aattataatg gtattaataa cgttgcaaaa gctgagcaaa cgacagataa
      181 cgaattgtgg aaaaatgtaa gagacgcttt aaaagaagcg aatattatcg ataaaacaga
      241 taatgaaaat gtcaaagtga cttataaatt agcaaatggc ggtgaaaaca gtattgaagg
      301 taccgccaat ttaaatgaac ttagcaattc taataacaaa ccgatcaata gcgatagtgt
      361 aaaaagcatt gatttgtcaa ggaaaaatcc caatggagat agatttgacg caaatgatgc
      421 atggaaaaaa ttaactgata aattaaaaga aaagcagatt gtgaaagacg gtgatacagt
      481 aactattcat agtaaagacg aaaaagaccc taaaatctca gctaaagtcg gtgaagtcta
      541 taatgataac aaacgattga tgttgtttaa aaaggatata gataaaatca ctataaacta
      601 gttatagata aagacaggtt acttttttaa tgtaacttgt ctttttttaa gaggcagtat
      661 ctcaaatatt aatttataat ttctaaatct atatctatac tttatctatt tctaaatcta
      721 tatctatact ttatctattt ctatatacac actcatccct cactgcaaca cagggcgttt
      781 ctcagcgtct taaaataaaa aaacgccact cgtaagtgac attaaaaaat atcttttata
      841 acatatccag tatttttgtt tgatctggaa aattcatttt tgtatatagg tcatctatag
      901 taattgtata gaaagaatgt gaataattac ttattagttt atccatattt ttcatcaatt
      961 ttatgtaatc atgtttattc aaaaatagac atagtgaaat caacaaatcg aatacataac
     1021 taccattcat tgtaggaacg tattctttat cgtatatttt gttataattg gcgaatatat
     1081 tagctctact tttagccctg tctattttat aatcatacaa tctttcttca tgcgcacaca
     1141 cgttacgaaa catatgtgct tgttgtagta tactgtctac atctgatgga gttatttgaa
     1201 cacgtgtttt ataatctctt tccaatttcc ttttatagtc tttagcaact tccaatcgaa
     1261 gatcatcatc caaattggaa tacatttttg aaacattacc taaagttaaa taattcacca
     1321 atatccatag tggcactcca ttatgagtat taatataatg ttttaatggt ttattttttc
     1381 tattactcat aaccgagcta aatgtagca
//
LOCUS       DAKTLH010000274         1406 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.283, whole genome shotgun sequence.
ACCESSION   DAKTLH010000274 DAKTLH010000000
VERSION     DAKTLH010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.283"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            48..1214
                     /gene="scpA"
                     /locus_tag="O0394_002825"
     CDS             48..1214
                     /gene="scpA"
                     /locus_tag="O0394_002825"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HCY0500830.1"
                     /translation="MKRNFPKLIALSLIFSLSVTPIANAESNSNIKAKDKKHVQVNVE
                     DKSVPTDVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVL
                     NTEGNIDYIVTISPKITKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVV
                     TQNHKAKLVLKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQ
                     YINKLENFKIRETQGNNGWCAGYTMSELLNATYNTNKYHAEAVMRFLHPNLQGQRFQF
                     TGLTPREMIYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGH
                     AMAVVGNAKLDNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYRWYSSIYGY"
     gene            1245..>1406
                     /locus_tag="O0394_002826"
     CDS             1245..>1406
                     /locus_tag="O0394_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HCY0500831.1"
                     /translation="MEQIELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICK"
BASE COUNT          547 a          199 c          219 g          441 t
ORIGIN      
        1 actaaattta atataattat tttataataa aataaaggag tttttatatg aaaagaaact
       61 ttccaaaatt aatagcttta agtttaatat tttctttaag tgttacccct attgcaaacg
      121 ctgagagcaa ttcaaatatc aaagcaaaag ataagaagca tgtgcaagtt aatgtcgagg
      181 acaagagtgt tcctactgac gtacgtaatt tagcgcaaaa agactatctt tcttatgtta
      241 cttcacttga taaaatctat aataaagaga aagcctcgta tacacttggt gaacctttta
      301 aaatttataa attcaacaaa aagagtgatg gtaattatta ttttccagtt ttgaacactg
      361 aaggtaatat cgattatata gttacaattt caccaaaaat cactaagtat tctagttcat
      421 cttctaaata cacaatcaat gtttctccat ttttatctaa agtcttaaat caatataaag
      481 atcaacaaat tactattttg acaaactcaa aaggttacta tgtggttact caaaatcata
      541 aagctaaact agtactaaaa acaccacgtc tagaagataa aaagttaaaa aagactgaga
      601 gtatacctac tggcaataat gtaacgcaac taaagcaaaa agcttctgtc actatgccaa
      661 ctagtcaatt taaaagtaat aattatactt acaacgagca atatataaat aaacttgaga
      721 atttcaagat aagagaaact caaggtaaca atggttggtg tgcaggctat acgatgtctg
      781 aattactaaa tgcgacttat aatactaaca aatatcatgc agaagctgtg atgagatttt
      841 tacacccaaa cttacaagga caacgattcc aatttactgg tttaacgcca agagagatga
      901 tttattttgg acaaactcag ggaagaagtc cacaacttct taatagaatg actacatata
      961 atgaagttga caatcttact aaaaataata aaggaattgc tgttttaggt agtagagtag
     1021 aatcaagaaa tggtatgcac gcagggcatg ccatggcagt tgttgggaac gctaaattag
     1081 ataatggaca agaggttata attatatgga atccatggga taatggtttt atgactcaag
     1141 atgctaaaaa caatgttatt ccagtatcca atggcgacca ttatcgatgg tattcatcta
     1201 tttatggtta ttaaaatttg tattttagaa aggtaaataa tattatggaa caaattgaat
     1261 tatttagtat tgataaattc aaatgtaatt cagaggctaa gtattatctt aatattattg
     1321 agggagaatg gcatcctcaa gatttaaatg atagtccttt aaaatttatt ctcagtacct
     1381 cagacgattc tgattacatt tgcaaa
//
LOCUS       DAKTLH010000275         1387 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.284, whole genome shotgun sequence.
ACCESSION   DAKTLH010000275 DAKTLH010000000
VERSION     DAKTLH010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.284"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..>1387
                     /locus_tag="O0394_002827"
     CDS             <1..>1387
                     /locus_tag="O0394_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lantibiotic dehydratase family protein"
                     /protein_id="HCY0500832.1"
                     /translation="LVSKEIIYSTIRPPLSYSDNLNYILNKLSLHNDDFVKKIREIQK
                     LILAYEKTEIGFGEELYKDIIHHMKALFKCKNYLQIDTKIDMINNYLHQDIATNISEA
                     AYLLWLLSRNNIGFTDLKVLHNRFIEKYGFEQLVNIKDLISDITGFGTTIFQEEETDG
                     NNIVMLKQKFLHALRNNDEIVINEKDVESLINDNEINHYHAPMSADVYAELYLGRFYN
                     QYNELIVISPLTASFNAGATFGRFHHLIDTETLAKLEHEKGHYYQKMICDDNVEMISI
                     NNIPKYPRNHNVLTNHDSYEYSLNLGSSNSYSKYELTLDDIYVGATFNKLYLYSSQLN
                     KRVLFESNNMYNFLKECNLYRLLREISMESVKCIEPMNDVSIDSFSYSPRIRYKNVIL
                     KPAYWKINEMVLPLPKNEEWDQQFLKYQEQFNIPNIVNLVYGDNKLLLNLSLANHRYL
                     LMKEYKKHKRVR"
BASE COUNT          540 a          174 c          212 g          461 t
ORIGIN      
        1 tcttgtctca aaggaaatta tatattcaac aatcagacca ccgttatcat atagcgataa
       61 tttaaattat attttaaata aactttcttt acataatgat gattttgtga aaaaaattag
      121 agaaatccag aaattgatat tagcttatga aaaaactgaa attggatttg gcgaggaatt
      181 atataaagac attattcatc atatgaaggc actattcaaa tgtaaaaact atcttcaaat
      241 tgataccaaa attgacatga ttaacaatta tttacatcaa gatatagcaa caaatatatc
      301 tgaagcagcg tacttattgt ggcttttatc aagaaataat attggattca cagatttgaa
      361 agttcttcac aaccgtttta ttgaaaagta tggatttgaa caactcgtga atattaaaga
      421 tttgatttca gatataacag gtttcgggac aacaattttt caagaagaag aaaccgatgg
      481 aaataatatt gtaatgctta agcaaaaatt tttacatgcc ttgagaaata acgatgagat
      541 agtcataaat gaaaaagatg tagagtcatt gattaacgat aatgaaatca atcattatca
      601 tgcgccaatg agtgcagatg tttatgctga actttattta ggacgttttt acaatcagta
      661 caatgagctc attgttatta gtcctttaac ggcgtctttt aatgctggcg ctacatttgg
      721 gcgatttcat catttaattg atactgaaac tttagcaaaa ttagaacatg aaaaaggaca
      781 ttattatcag aagatgatat gtgatgacaa tgtagaaatg atttctataa ataacatacc
      841 gaaatatccg agaaatcata atgtattaac taatcatgac tcatacgaat attcattgaa
      901 tttaggaagt agtaatagtt attcaaagta tgagcttacc ttagatgata tttatgttgg
      961 tgctaccttt aacaaattat atttatattc tagccaacta aataaaaggg tactatttga
     1021 atcaaacaat atgtataact ttttaaaaga atgtaattta tatcgattgt tacgagaaat
     1081 ttcaatggaa tctgtgaaat gcattgaacc tatgaatgat gtgagtattg attcatttag
     1141 ttattcacca cgaatcagat ataaaaatgt cattcttaaa ccagcatatt ggaaaattaa
     1201 cgagatggta ttacctttac ctaaaaatga agaatgggat caacaatttt tgaaatatca
     1261 agaacaattc aatataccta atatcgtgaa tttagtttat ggagataaca agcttctact
     1321 aaatttatca ttagctaatc atagatattt attgatgaaa gaatataaga agcataaacg
     1381 tgtacgt
//
LOCUS       DAKTLH010000276         1370 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.285, whole genome shotgun sequence.
ACCESSION   DAKTLH010000276 DAKTLH010000000
VERSION     DAKTLH010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.285"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..320)
                     /locus_tag="O0394_002828"
     CDS             complement(<1..320)
                     /locus_tag="O0394_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0500833.1"
                     /translation="MLLCDVRVIYKNPKYKVIQHNGEYLLVDLVSTWFVYFFPFINWF
                     IPKKYAIISEEEFENLNVVKPNKNNVFWSVIGSSVLFGVTLRKYIHVFDVQLDKLVVM
                     ILCAL"
     gene            complement(773..1370)
                     /locus_tag="O0394_002829"
                     /pseudo
     CDS             complement(773..1370)
                     /locus_tag="O0394_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837136.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF443 family protein"
BASE COUNT          501 a          239 c          146 g          484 t
ORIGIN      
        1 agagcacaca atatcattac aactagctta tctaattgaa catcaaaaac atgtatgtat
       61 ttccttaaag taactccaaa caaaaccgaa cttcctataa ctgaccagaa aacattattt
      121 ttatttggtt taacaacatt taaattttca aattcttctt cgctaattat cgcgtacttt
      181 tttggaatga accaattaat gaaaggaaaa aagtacacga accaagtgct tactaaatcg
      241 actaataagt attcaccgtt atgttgaatg actttgtatt tcggattttt ataaatgact
      301 ctgacatcgc aaagcaacat ttaccactcc cgtttcatta gttttaaatg atccccaata
      361 ctttttgtac cttcaccaac tatcgttcca acactatcta cagctttttc tgtggtattt
      421 ttaaacagcg tctatttaat gtcttatcca attagtttta tctcttaatc caaatttatt
      481 agtatgaaaa gtccaatttt taagaattgt ctaaacagat tccgcaacaa aaccagctaa
      541 aaccgtccat actattggat taaaaacgcc aatgttataa gagctatcat acaaaataac
      601 acgctctata tttcattttt aaattaaaac agttttttcc tttatcaatc acaatgggca
      661 ttttcacttg gtatattcaa acattactat ctattttcct gatttaagat atgattatat
      721 ttttttgaac caccgtttaa atatgcgata ctaccaattt cgatcatttt tactactcgt
      781 ttattcttaa aataacatga acatttttat tacctatcga agccatattc actaaaaaga
      841 aaaacatcgt gataataacc caagttaaaa atattattat attttgtgac tctgtcgtca
      901 aaagaatggt taaagtaaat attgaagcgc ttcctaaaaa aaagtacgca aaaactatta
      961 acaatccttg tttgaaagtc ggtattaata caactctatt cttattaacc acgttggtat
     1021 taaaaatttt taatgttaat tttttattta gataaaaata aaaaattaac atccctatta
     1081 acatgataaa aaatacagtg attgctagtt gtttttcaaa ctgaacatta aagaagttac
     1141 cgtactttct caatataatt ccaaatagaa ctgaacttcc tgcaaccggc caaaaaacat
     1201 tatttttaac aggctcgact atatttaacc tttaaaattc attttcgcta atttttgcgt
     1261 attttttggg caaaaaccaa ttaatcatag ggaaaaaata agtaatccaa gtacttacta
     1321 aatcgaccat gaaatattca ttattatatc taataactcg atatttagga
//
LOCUS       DAKTLH010000277         1352 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.286, whole genome shotgun sequence.
ACCESSION   DAKTLH010000277 DAKTLH010000000
VERSION     DAKTLH010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.286"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            50..1306
                     /locus_tag="O0394_002830"
     CDS             50..1306
                     /locus_tag="O0394_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HCY0500834.1"
                     /translation="MIDNFMQVLKLIKEKRTNNVVKKSDWDKGDLYKTLVHDKLPKQL
                     KVHIKEDKYSVVGKVATGNYSKVPWISIYDENITKETKDGYYLVYLFHPEGEGIYLSL
                     NQGWSKISDMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAENKDSSYDLKND
                     YPSGYSHGSIRFKYYDLNEGFTEEDMLEDLKKFLELFNELASKVTKTSYDSLVNSIDE
                     IQEDSEIEEIRTAQKDKTLKEVEAPKGIIPKYKKGVSKTTKNDSEIEKSNKENKLTGK
                     VGEKLALNYFNELIDNKIDEDKKEQFRNILNDNPGSQHGHGYDLVAFDPTNTDKAVEK
                     FIEIKTSTSSSIEEPFFMSLNEMFAMKEYKQKYLILRIFNVSGKEPQFYFIDPYANYS
                     EFKDVDDLIDKVFNVEAIQYKVFGEK"
BASE COUNT          575 a          151 c          228 g          398 t
ORIGIN      
        1 attgaagaaa atgagacata atatatttta taataggagg gaatttcaaa tgatagacaa
       61 ctttatgcag gtccttaaat taattaaaga gaaacgtacc aataatgtag ttaaaaaatc
      121 tgattgggat aaaggtgatc tatataaaac tttagtccat gataagttac ccaagcagtt
      181 aaaagtgcat ataaaagaag ataaatattc agttgtaggg aaggttgcta ctgggaacta
      241 tagtaaagtt ccttggattt caatatatga tgagaatata acaaaagaaa caaaggatgg
      301 atattatttg gtatatcttt ttcatccgga aggagaaggc atatacttat ctttgaatca
      361 aggatggtca aagataagtg atatgtttcc gcgggataaa aatgctgcaa aacaaagagc
      421 attaacttta tcttccgaac tcaataaata tattacatca aatgaattta atactggaag
      481 attttattac gcagaaaata aagattcatc ttatgattta aaaaatgatt atccatcagg
      541 atattctcat ggatcaataa gattcaaata ttatgatttg aatgaaggat tcacagaaga
      601 agatatgcta gaggatttaa agaaattttt agaactattt aatgaattag cttcaaaagt
      661 tacaaaaaca tcctatgata gcttggtcaa tagcatagac gaaatacagg aagacagcga
      721 aattgaagaa attagaacag cacaaaaaga taagacactc aaggaagtgg aagcacctaa
      781 aggaataatt ccaaaatata aaaaaggtgt atcaaagact actaaaaatg attcagaaat
      841 tgaaaaatca aataaagaga ataaattaac cggtaaagtt ggagaaaaat tagcgctaaa
      901 ttactttaat gagctaattg ataataaaat agacgaagat aagaaagaac agtttaggaa
      961 tattttaaat gataatccag gctctcaaca cggtcatggc tatgatttag tagcttttga
     1021 tccaacaaat acagataaag ctgtagaaaa atttattgaa attaaaacat ctacatcttc
     1081 tagtattgag gaaccatttt ttatgtcgct aaatgaaatg tttgctatga aagaatataa
     1141 gcagaaatat ttaatattaa gaatatttaa tgtttccggt aaagaaccac aattttattt
     1201 tatagatcca tatgcaaatt attctgaatt taaagatgta gatgatctca ttgacaaagt
     1261 atttaatgta gaagctattc agtataaagt ttttggcgaa aaatgattac ttgaacaaga
     1321 gctaaaataa aattgtgatc taataaaaat ag
//
LOCUS       DAKTLH010000278         1342 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.287, whole genome shotgun sequence.
ACCESSION   DAKTLH010000278 DAKTLH010000000
VERSION     DAKTLH010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.287"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            27..743
                     /locus_tag="O0394_002831"
     CDS             27..743
                     /locus_tag="O0394_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0500835.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            743..1216
                     /locus_tag="O0394_002832"
     CDS             743..1216
                     /locus_tag="O0394_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCY0500836.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEISNTFSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
BASE COUNT          468 a          182 c          283 g          409 t
ORIGIN      
        1 aaacagttcg aaagcgagat gacattatgg gacgtaaatg gaataacatt aaagaaaaaa
       61 aggcccaaaa agataaaaac acaagtagaa tatatgcgaa atttggtaag gagatttatg
      121 ttgcagcaaa atctggtgaa cccaatccag aatctaacca agctttaagg ttggtgcttg
      181 aacgcgctaa gacatattca gtgccgaatc atattattga aaaagcaata gataaagcta
      241 agggtgctgg agacgaaaac tttgatcacc taagatatga aggatttggc ccaagcggat
      301 caatgctaat tgttgatgcg ttaacaaata atgtaaatcg tactgcctct gatgtgcgag
      361 ctgcttttgg taaaaacggc ggtaatatgg gtgtatctgg atcagttgct tatatgtttg
      421 atcatgtggc aacatttggt attgaaggaa agtctgttga cgaaatactt gaaacattaa
      481 tggaacaaga tgtagatgta aatgatgtga ttgacgataa tggattgaca atagtctatg
      541 ctgaaccaga tcaatttgca gtcgttcaag atgcgcttcg tgcagcaggt gttgaagaat
      601 ttaaagttgc tgaatttgaa atgttacctc aaacagatat tgaactttct gaagcggacc
      661 aagtaacatt tgaaaaatta atcgatgcat tagaagattt agaagatgta caaaacgtat
      721 tccataatgt ggatttgaaa taatgaaatc agcagaacaa tggattgatg aattgcaact
      781 tgaatcacat cctgaaggtg gtttctatag agagacaatt cgagaagtat tgaaagatgg
      841 acgcagagcg ccgtttagta gtatttattt tttacttaca gatgacaata tttcgcattt
      901 tcatcgaatt gatgctgatg aagtatggta ctatcatgct ggcgattctc taacaattca
      961 tatgataaat ccggatgggg aatatacgac tgcaacattg ggtactgata tccaaaatgg
     1021 agatgtattg caatatgtag tgcctaaagg aacaattttt gcttcttcaa tcgaaatatc
     1081 aaatactttt agtttagtag gttgtatgtg tcaaccggca tttgagttta agcagtttga
     1141 attgtttaag caatctgaat taattacgca atatccgcat cttaaatcag taatcgaaaa
     1201 atatgcttta aaataaaagt gatcaatgaa gtggtttgaa ggttgttaat aaacctttga
     1261 gtcacttcat ttttatatgt attcttgatt gaatcagaat agatttgatg cttcagctgt
     1321 ttttaatgaa aatagcatta aa
//
LOCUS       DAKTLH010000279         1304 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.288, whole genome shotgun sequence.
ACCESSION   DAKTLH010000279 DAKTLH010000000
VERSION     DAKTLH010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.288"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            171..1109
                     /gene="lukS-PV"
                     /locus_tag="O0394_002833"
     CDS             171..1109
                     /gene="lukS-PV"
                     /locus_tag="O0394_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     S"
                     /protein_id="HCY0500837.1"
                     /translation="MVKKRLLAATLSLGIITPIATSFHESKADNNIENIGDGAEVVKR
                     TEDTSSDKWGVTQNIQFDFVKDKKYNKDALILKMQGFINSKTTYYNYKNTDHIKAMRW
                     PFQYNIGLKTNDPNVDLINYLPKNKIDSVNVSQTLGYNIGGNFNSGPSTGGNGSFNYS
                     KTISYNQQNYISEVERQNSKSVQWGIKANSFITSLGKMSGHDPNLFVGYKPYSQNPRD
                     YFVPDNELPPLVHSGFNPSFIATVSHEKGSGDTSEFEITYGRNMDVTHATRRTTHYGN
                     SYLEGSRIHNAFVNRNYTVKYEVNWKTHEIKVKGHN"
     gene            1105..>1304
                     /locus_tag="O0394_002834"
     CDS             1105..>1304
                     /locus_tag="O0394_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucotoxin LukD"
                     /protein_id="HCY0500838.1"
                     /translation="MDMKKIVKSSVVTSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDSDKLKISQILTFNF"
BASE COUNT          523 a          172 c          204 g          405 t
ORIGIN      
        1 ttataatttg tttggtaatg aacgggtttt tttcgaaata atagtaaaaa aacacatttg
       61 tagatatttt aaactcggta aatcttttaa taaatattta attttattaa aagttaaaaa
      121 ggtttaatat aaaaatgtaa taaaatttat aaagaaagga aatgattttt atggtcaaaa
      181 aaagactatt agctgcaaca ttgtcgttag gaataatcac tcctattgct acttcgtttc
      241 atgaatctaa agctgataac aatattgaga atattggtga tggcgctgag gtagtcaaaa
      301 gaacagaaga tacaagtagc gataagtggg gggtcacaca aaatattcag tttgattttg
      361 ttaaagataa aaagtataac aaagacgctt tgattttaaa aatgcaaggt tttatcaatt
      421 caaagactac ttattacaat tacaaaaaca cagatcatat aaaagcaatg aggtggcctt
      481 tccaatacaa tattggtctc aaaacaaatg accccaatgt agatttaata aattatctac
      541 ctaaaaataa aatagattca gtaaatgtta gtcaaacatt aggttataac ataggtggta
      601 attttaatag tggtccatca acaggaggta atggttcatt taattattca aaaacaatta
      661 gttataatca acaaaactat atcagtgaag tagaacgtca aaattcaaaa agtgttcaat
      721 ggggaataaa agctaattca tttatcacat cattaggtaa aatgtctgga catgatccaa
      781 atttatttgt tggatataaa ccatatagtc aaaatccgag agactatttt gttccagaca
      841 atgaattacc cccattagta cacagtggtt tcaatccttc atttattgca actgtttctc
      901 atgaaaaagg ctcaggagat acaagtgaat ttgaaataac gtatggcaga aatatggatg
      961 ttactcatgc tactagaaga acaacacact atggcaatag ttatttagaa ggatctagaa
     1021 tacacaacgc atttgtaaac agaaattaca cagttaaata tgaagtgaac tggaaaactc
     1081 atgaaattaa agtgaaagga cataattgat atgaaaaaaa tagtcaaatc atcagttgtt
     1141 acatcaattg cattgctttt gctatccaat acagttgatg cagctcaaca tatcacacct
     1201 gtaagtgaga aaaaggttga tgataaaatt actttgtaca aaacaactgc aacatcagat
     1261 tccgataagt taaaaatttc tcagatttta acttttaatt ttat
//
LOCUS       DAKTLH010000280         1297 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.289, whole genome shotgun sequence.
ACCESSION   DAKTLH010000280 DAKTLH010000000
VERSION     DAKTLH010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.289"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            77..1036
                     /gene="hyl"
                     /locus_tag="O0394_002835"
     CDS             77..1036
                     /gene="hyl"
                     /locus_tag="O0394_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HCY0500839.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWIDRSSERYKIDWEKEEMTN"
BASE COUNT          491 a          188 c          215 g          403 t
ORIGIN      
        1 tcaactgcaa tattctaaat tgacatattg attattgttt cctcaaatcg ttttaaaaaa
       61 tagaaggatg atgaaaatga aaacacgtat agtcagctca gtaacaacaa cactattgct
      121 aggttccata ttaatgaatc ctgtcgctaa tgccgcagat tctgatatta atattaaaac
      181 cggtactaca gatattggaa gcaatactac agtaaaaaca ggtgatttag tcacttatga
      241 taaagaaaat ggcatgcaca aaaaagtatt ttatagtttt atcgatgata aaaatcataa
      301 taaaaaactg ctagttatta gaacgaaagg taccattgct ggtcaatata gagtttatag
      361 cgaagaaggt gctaacaaaa gtggtttagc ctggccttca gcctttaagg tacagttgca
      421 actacctgat aatgaagtag ctcaaatatc tgattactat ccaagaaatt cgattgatac
      481 aaaagagtat atgagtactt taacttatgg attcaacggt aatgttactg gtgatgatac
      541 aggaaaaatt ggcggcctta ttggtgcaaa tgtttcgatt ggtcatacac tgaaatatgt
      601 tcaacctgat ttcaaaacaa ttttagagag cccaactgat aaaaaagtag gctggaaagt
      661 gatatttaac aatatggtga atcaaaattg gggaccatat gatagagatt cttggaaccc
      721 ggtatatggc aatcaacttt tcatgaaaac tagaaatggc tctatgaaag cagcagataa
      781 cttccttgat cctaacaaag caagttctct attatcttca gggttttcac cagacttcgc
      841 tacagttatt actatggata gaaaagcatc caaacaacaa acaaatatag atgtaatata
      901 cgaacgagtt cgtgatgact accaattgca ctggacttca acaaattgga aaggtaccaa
      961 tactaaagat aaatggatag atcgttcttc agaaagatat aaaatcgatt gggaaaaaga
     1021 agaaatgaca aattaatgta aattatttgt tcatgtacaa ataaatataa tttataactt
     1081 tagccgataa cttcagaaat gatgtttttc ggctaatttt tatactgaat ttagtttaat
     1141 taacttatga tatttttaat ttaaactatc tacttgattt gctttcctga cttaaaaatt
     1201 agactaaaca tgacactata tgaggtaatt tttaaaataa gaaatttgaa ttccatgttg
     1261 tcaaaccctt tatataaaag ttttgaaatt gttcaaa
//
LOCUS       DAKTLH010000281         1283 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.290, whole genome shotgun sequence.
ACCESSION   DAKTLH010000281 DAKTLH010000000
VERSION     DAKTLH010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.290"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..1113)
                     /locus_tag="O0394_002836"
     CDS             complement(<1..1113)
                     /locus_tag="O0394_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0500840.1"
                     /translation="MLYLEVLKNRNFTYLLIGNFLRRSCFVLFSLQIIWFTVELTNQS
                     SLKLSMMVMSQTLPFIIFGIFGGAYSDKHNKKKILYLSDLILSLIIIIIPLLAITSNL
                     NYLTLLTISTAITIINCYTDPAFRAILPEIIDEEHLATSNALIDSLQRGSNIILPALI
                     GVIVILVGNVGVFFICSILLFLGFIFNALLKYTNNNMIDRHSKEDFSETWEFLKQSKE
                     IPFIIIIQFACILINTGLWRVVLPLFISNILKEGVGVYGLATSCLGIASLLMSLIMGL
                     LSEKRLIFKFSIGVLVWGIGLSIINVFPSVAILYIGATLLGLGQSIEGLTRSVAIQIK
                     TPNHLMGKVFSISSTSNYAADTLSLGLISIIIPLLSL"
BASE COUNT          493 a          224 c          159 g          407 t
ORIGIN      
        1 taagcttagt aaaggtataa ttatacttat cagtcctaaa gacaatgtat ccgctgcata
       61 attcgatgta gatgagatac taaatacttt ccccatcaaa tgatttggcg tttttatttg
      121 aatggcaact gatcttgtta gtccctctat agattgtcca agtcccaata atgttgcacc
      181 tatatataat atcgccacac ttggaaacac attaataatc gataagccaa ttccccaaac
      241 taaaacacca atactaaatt taaagattaa tcgcttttct gacagcaaac ccataatcaa
      301 tgacattaat aaagatgcta tacctaaaca tgatgtagct agtccatata caccaacgcc
      361 ctcttttaat atattggaaa taaacaatgg taatacaaca cgccaaaggc cagtattaat
      421 caatatgcag gcaaattgga tgattataat aaatggaatt tctttagatt gtttcaagaa
      481 ttcccaagtt tcagaaaaat cttcttttga gtgtctatca atcatgttgt tatttgtata
      541 ttttaaaagt gcattaaaaa taaatcctaa aaatagcaat atactacaaa taaaaaagac
      601 gccaacatta ccaactagta ttacaatgac accaattaaa gcaggtaaaa taatatttga
      661 gcctctttgc aaactatcga ttaacgcatt acttgttgct aaatgctcct catcaataat
      721 ttcaggaaga attgccctaa acgcaggatc cgtatagcag ttaataatgg tgatagctgt
      781 agatatggtt agaagcgtca gataatttaa atttgatgtt attgcaagta aaggaataat
      841 tattataatc aaacttagta taagatcaga tagataaagt attttctttt tattatgttt
      901 atcagaatat gcgccaccga aaataccaaa tataataaat ggaagtgttt gactcataac
      961 catcattgat aattttaaag atgattggtt tgtcaattca acagtaaacc aaattatttg
     1021 taacgaaaac agcacaaaac aactccgacg taagaaatta ccaatcaata aatatgtaaa
     1081 gtttctattt ttcaaaactt ctaaatacaa catatttatc acctctcata aaaataattg
     1141 aatgcatcca ccagcttttt tagaccttct tctaaactct ctttatccaa agcgcaatta
     1201 attctaatat aatttagtca gttaaatatc aattatttcg aaatatacat actacttgaa
     1261 acaccataca taacccccaa aat
//
LOCUS       DAKTLH010000282         1274 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.291, whole genome shotgun sequence.
ACCESSION   DAKTLH010000282 DAKTLH010000000
VERSION     DAKTLH010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.291"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(14..1180)
                     /gene="norA"
                     /locus_tag="O0394_002837"
     CDS             complement(14..1180)
                     /gene="norA"
                     /locus_tag="O0394_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HCY0500841.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLILLVFANDYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
BASE COUNT          469 a          255 c          170 g          380 t
ORIGIN      
        1 caaaatactt atgctacata ttttgttctt tcaattttgc tctatgttgc ttttcaatta
       61 aaacaataac aacacctgct aatgaaacac ctatagccat ataaattggt gcttcaatgt
      121 gtacatcaaa taacgcacct gcgattaaag gacctatgaa attacccata ctagtgaatg
      181 tcgagttcaa tccgcctgca aagccttgcc tttctccagc aatattagaa aaataatttg
      241 taatggctgg tcgtatcata tcaaaaccta tgaagacaac aaaactgatt aacattattg
      301 accaatagtc attagcaaaa actaataata ttaagacaac aactgaatat aataatgacc
      361 aagctataaa tgttaactct gagaaatact tcataaattt atcgaagaaa tagatttgga
      421 aaagtgcccc aaatataccg ccacccgtaa tagcaatcga aatatcttta ggtgaataat
      481 ttaccttgtc agctgtgtat agtgaataca atgtttcaaa tgcagataaa ccaaacgata
      541 atacaagtgt taaaataact ggtgtaataa acactttcca gttaattttc gttagcaatt
      601 gtggctctaa cttttggaaa ccacttgtcg tagacttttt cggatcgtga atcaatacaa
      661 ttgacattat aaatgctaga atacctaatg ctcctgcaaa gtaaaatggc atacgatgtg
      721 aaacttctgc cataaatcca ccaatccctg gtcctaaaat gaatccagaa ttgataatcg
      781 ctgacatgta gccaaagttt tttgcttttt gatggcttgg tgaaatgtca gctattaaac
      841 ctgtcacacc aggcattacc ataccagcac tcataccacc aatcactctc gataacatca
      901 ataccgaaaa attgtggcca actgcaaaca taaattctga cactgaaaac aaaattaatc
      961 ctatacatat aattaatttc ttccctaatt tgtcagctag cgtaccacca aacggcgata
     1021 taatcatttg agataacgca aaagcagcaa ctagtaatcc taaatcacta ccagttaatc
     1081 ccaaatcttt taaataaaca ggcaagactg gtattactaa accgatacct aaaaaaatca
     1141 agaaaatatt aaaatataag acaaaaatct gtttattcat atgctcacct cttttttctc
     1201 catgtcatgc ttaaagctgt tattcttctt ttccacgaca gattgcaatt catgctaaat
     1261 attcgtaaaa agtt
//
LOCUS       DAKTLH010000283         1254 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.292, whole genome shotgun sequence.
ACCESSION   DAKTLH010000283 DAKTLH010000000
VERSION     DAKTLH010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.292"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            123..1237
                     /locus_tag="O0394_002838"
                     /pseudo
     CDS             123..1237
                     /locus_tag="O0394_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT          506 a          175 c          187 g          386 t
ORIGIN      
        1 aaaaaataag acactttgcc aaacttgcac ataaatgttt aattcaataa tttgaatttt
       61 ctgtgttggg tcccttcgta taatttaata aataccacta aactaaatta atgaagtgcc
      121 ttatgtataa aaattataac ttgatccaac ttacactacc aatagaaact tctgttagaa
      181 ttcctcaaat tgatatttcg cgatatgtta atgaaattgt tgaaacgata cctgatagcg
      241 aattcgataa attcagacat catcgtggcg caacatccta tcatccaaaa atgatgttaa
      301 aaatcacctt atatgcatat actcaatctg tattttctgg tcgtagaata gagaaattac
      361 ttcatgacag tattcgaatg atgtggttag ctcaaaatca aacaccttct tataaaacta
      421 ttaatcgtat tagagtgaat cctaatactg atgcgttaat tgaatcttta tttatccagt
      481 ttcatagtca atgtttaaag caaaatctta ttgatggtac aaaagtagaa gctaatgcca
      541 ataggtatac atttgtgtgg aagaaaagta ttcaaaatca cgaatcgaaa ttgaacgaaa
      601 attcaaaaac attatatcgt gacttagttg aagaaaaaat aataccagag ataaaagaag
      661 atggagatag cgatttaaca atagaagaaa tagatttaat tggtagtcat ttagataaag
      721 aaatcgaagg tttaaatcat tctattgaga acgaagatag tactcaaatt agaaaacaga
      781 cccgtaaaaa agaactaaga ttaagaagtt caaaaataaa tttgatgatt attccgaaag
      841 aaaaaataaa tatgaagaac aaaaatcgat tcttaaagat agaaatagct tttctaaaac
      901 tgatcatgat gcaactttta tgagaatgaa ggaagaccat atgaaaaatg gccaacttaa
      961 gccaggatac aatttacaaa tagcgacaaa ttctcaattt gttttatcct atgacctatt
     1021 tcaaaaccca acagatacta gaactttaat tccattttta acaatgattc aaaatacctt
     1081 cggttattta ccggagtata ttgtagctga tgcaggttat ggtagtgagc aaaactatat
     1141 ggctattata gatgatttta ataaaacgcc acttattacg tatggcatgt ttattaaaga
     1201 taaaactaga aagtttaaaa gtgacatttt caacacttaa aattggaaat atga
//
LOCUS       DAKTLH010000284         1238 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.293, whole genome shotgun sequence.
ACCESSION   DAKTLH010000284 DAKTLH010000000
VERSION     DAKTLH010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.293"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..408)
                     /gene="sarU"
                     /locus_tag="O0394_002839"
     CDS             complement(<1..408)
                     /gene="sarU"
                     /locus_tag="O0394_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HCY0500842.1"
                     /translation="MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFY
                     YKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRK
                     KIACVINELDKIIKGFNKERDYIKYQWAPKYSKE"
     gene            737..1093
                     /gene="sarT"
                     /locus_tag="O0394_002840"
     CDS             737..1093
                     /gene="sarT"
                     /locus_tag="O0394_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarT"
                     /protein_id="HCY0500843.1"
                     /translation="MNDLKSKSNIKLMKRVLTTYELRKYLKKYFCLTLDNYLVLAYLD
                     VFKNDEGKYFMRDIISYIGIDQSRIVKSVKDLSKKGYLNKCRDPHDSRNVIIVVSVKQ
                     HNYIKNLLSEININET"
BASE COUNT          470 a          138 c          160 g          470 t
ORIGIN      
        1 ttctttgcta tattttggag cccattgata ttttatgtag tctctttcct tattaaatcc
       61 tttaattatt ttatctaact cattaataac acaagcaatc tttttacgtt gtattggagt
      121 aactgaaaca aaaatacggc gttcatctgc ttcgtttcta gacttattta taaatccttt
      181 tttagatagt gacttaatgt tctttacaac atcagattgt ttgtaaagta tgtcaccaat
      241 gatttctttt agtgaaatac tgtgttcatt tttgtaataa aaatatgcta atatcaacaa
      301 ttcttttaat gataatttat attgttgctt taactcttga gtgagaaaaa atatgtacgc
      361 ttctacattt atgaatttat tgaccttttc gaaagtttga tagtccacct tacattctcc
      421 tactattttc tgatgtccca atactttata ttatttaaag taatataaaa gcaatctctt
      481 ttaaagacat gttaacctaa atattttata tataatatat atatatataa aatataaata
      541 actaaaaatc acagaataac aaatgacaaa ttcatttatt atccaaaaat aaaaacaatt
      601 gaacaaaatc aaaatatagt gttatgattc atttgtgttt atagctagca ccacaaacta
      661 aatatatttg gagttattac tagttaaaga caaatattat ttgacgtgac aattaaaagt
      721 aagggatgaa gacttgatga atgatttgaa aagcaagagc aatattaaat taatgaaaag
      781 agtattaact acatatgagc tgcgtaaata tttaaaaaaa tatttttgtc ttacattaga
      841 taattattta gtattagcat atttagatgt atttaaaaat gatgaaggta aatattttat
      901 gagagacatt ataagttata tcggtataga ccaatctaga attgttaaaa gcgtaaaaga
      961 tttatcaaaa aagggttact tgaataaatg tagagaccca catgatagta gaaatgtaat
     1021 cattgttgtt tctgtaaaac aacacaatta tattaaaaat cttttgagtg aaataaatat
     1081 taatgaaaca taaagtatgt ttcgagattt taatatcttg gtgcagttaa atgtattttt
     1141 taaatgcgtt aaggtatttt ttattttaga aattcaatgc tattgagttg atgggttttt
     1201 ccaacagata agaatgaatt attcattgaa aactaaaa
//
LOCUS       DAKTLH010000285         1229 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.294, whole genome shotgun sequence.
ACCESSION   DAKTLH010000285 DAKTLH010000000
VERSION     DAKTLH010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.294"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..240
                     /locus_tag="O0394_002841"
     CDS             <1..240
                     /locus_tag="O0394_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCY0500844.1"
                     /translation="HHGSKTSSSKEFIEMIKPKISLISSGKNNMYHLPNIEVVKRLQR
                     IRSRIYNSQQNGQVTIDLDDNLKVDSSSYGNASGL"
     gene            297..>1229
                     /gene="holA"
                     /locus_tag="O0394_002842"
     CDS             297..>1229
                     /gene="holA"
                     /locus_tag="O0394_002842"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCY0500845.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIIDACAETDYKLKSSYM"
BASE COUNT          515 a          135 c          212 g          367 t
ORIGIN      
        1 catcatggga gcaagacaag tagttctaaa gaatttatag agatgattaa gcctaaaata
       61 agtttgattt cttctgggaa gaacaatatg tatcatcttc ctaatataga agttgttaaa
      121 cgattgcaaa ggattcgcag tcgcatttac aatagtcaac aaaacggtca agttacaatt
      181 gacttagatg ataatttaaa agttgattca agctcttatg gaaatgcaag tggtttatag
      241 atacagaaac atgatataat ctattgatgt gtcaaacgaa tgaaagggtg taatgaatga
      301 gcgacaatat tgtagctatt tatggagatg tgcctgaatt ggttgaaaaa caaagtgcag
      361 aaatcatatc acaatttttg aaaagtgata gagatgactt taactttgtg aaatataatt
      421 tatacgaaac agagattgca ccaattgttg aagaaacatt aacattgcct ttcttttcag
      481 ataaaaaagc aattttggtt aaaaatgcat atatatttac aggtgaaaaa gcgccaaaag
      541 atatggctca taatgtagat caattaatag aatttattga aaaatatgat ggcgaaaatt
      601 tgattgtctt tgagatatat caaaataaac ttgatgaaag aaaaaagtta actaaaactc
      661 taaaaaagca tgcaaggctt aaaaaaatag agcaaatgtc tgaagaagaa ataaaaaaat
      721 ggattcaaag taaattaaat gagaatttca aagatatcaa aagagatgca ttagatttat
      781 ttattgagtt gacaggtatt aactttaata ttgtctcaca agagatagaa aagttgattt
      841 tatttttagg cgatagacca acaattaata agcaggatgt taaccaaatt attaatagaa
      901 gtttagagca aaatgtattt ttactgactg aatacattca gaaaagaaag aaagaacaag
      961 caattcattt agtaaaagat ttaataacta tgaaagaaga accaattaaa ttacttgcac
     1021 taattacaag taattaccga ttattttatc aatgtaagat tctgagtcaa aaaggatata
     1081 gtggacagca aattgctaaa acaataggag tacatccata cagagtaaaa ttagcgttag
     1141 gacaagtaag acattatcaa cttgatgaat tattgaatat tatagatgct tgtgcggaaa
     1201 ctgattataa acttaaatca tcatatatg
//
LOCUS       DAKTLH010000286         1146 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.295, whole genome shotgun sequence.
ACCESSION   DAKTLH010000286 DAKTLH010000000
VERSION     DAKTLH010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.295"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..>1146
                     /locus_tag="O0394_002843"
     CDS             <1..>1146
                     /locus_tag="O0394_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCY0500846.1"
                     /translation="KLEFNISNLASRYTANNDVDTINNKIIEGISITNNFKYTNISYL
                     KELKGYIENDILDKMKSKYAKNIYLNFKKIFSNLEQSVNNYLELEEEFEEKKKYLSEI
                     YELIDEINIDPYIFLTEHNECNIYKISENEKLELQTYMSKIEKVLLKYQTYLKETCKE
                     CLFYPYLLVQGEAGIGKSHLLAHLSKKLRDENHIIYLFLGQFFTKNEDPWHQILNDLE
                     VTNSVDNFLRSISNKAKETKKRAFIIIDALNEGEGKRLWGNYFQSFINHIKKYSNIAL
                     IFSIRTPFEDVILPKNAIQDNNIVVFQHEGFSKEENYNPIVSFCDFYGLELPKLPILN
                     PEFNNPLFLKLMCEYCVNKFKEFDQTISVAELFTNVLKTVNINLSKE"
BASE COUNT          475 a          122 c          161 g          388 t
ORIGIN      
        1 aaaaattaga atttaatata tcgaatttag cttcacgata tactgcgaat aatgatgtcg
       61 atactattaa taataaaatt atagaaggta tttctattac caataatttt aaatatacta
      121 atatttcata tctaaaagaa ttaaaaggtt atatcgaaaa tgatatttta gataagatga
      181 aatcaaaata cgcaaagaat atttatttga atttcaaaaa aatattctct aatttagaac
      241 aatcagtcaa taattatttg gaactggaag aagagtttga agaaaagaaa aaatacttaa
      301 gtgaaattta tgaattgata gacgaaatta atattgaccc ttatattttt ctgacagaac
      361 ataatgaatg taatatatac aaaataagtg agaatgaaaa attagaactt caaacttata
      421 tgagtaaaat cgaaaaagta ttattaaaat atcaaacgta tttaaaagaa acatgtaaag
      481 aatgcttgtt ttatccttat ttgctagttc aaggtgaagc tggcatagga aaatcacacc
      541 ttttagcgca tctatctaaa aaacttagag atgaaaatca tattatttat ctgtttttgg
      601 gacaattttt tactaaaaat gaagatccat ggcatcaaat acttaatgat ttagaagtta
      661 caaattctgt tgataatttt ttaaggtcaa taagtaataa ggccaaagaa acaaaaaaaa
      721 gagcttttat tattattgat gcgcttaatg aaggtgaagg taaaaggtta tggggaaatt
      781 attttcaaag ctttatcaac catataaaga agtattctaa tatagcttta atattttcta
      841 ttagaacccc ttttgaagat gttatattac ccaaaaacgc aatacaagat aacaatattg
      901 tagtatttca gcatgaaggt tttagtaagg aagaaaacta taatccaatt gtatcttttt
      961 gtgattttta tggattagag ctacctaagt tacctatatt aaatccagaa ttcaacaatc
     1021 cattattttt aaaactaatg tgtgagtact gtgtaaataa atttaaggaa tttgaccaaa
     1081 ctataagtgt tgcagagcta tttacaaatg ttcttaagac agtaaatatt aatttatcta
     1141 aggaag
//
LOCUS       DAKTLH010000287         1121 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.296, whole genome shotgun sequence.
ACCESSION   DAKTLH010000287 DAKTLH010000000
VERSION     DAKTLH010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.296"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(94..308)
                     /locus_tag="O0394_002844"
                     /pseudo
     CDS             complement(94..308)
                     /locus_tag="O0394_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736766.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
     gene            295..1121
                     /locus_tag="O0394_002845"
                     /pseudo
     CDS             295..1121
                     /locus_tag="O0394_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          429 a          169 c          185 g          338 t
ORIGIN      
        1 aaggtataat gtttgcggta aaaataaaga cgaagtgctg gaatacactt cgtcgataat
       61 aaggtagttg aagtttcgat tattttaaat tgatcatttt aagcctacaa atccctctaa
      121 tgtttcacca gatttgtaca cttttgtcgc cttctttttc ctaaataacg taaatgccaa
      181 ggttcatatt gatatcctgt gatgctttct ttgtttttag gatatcttat aataaatcca
      241 taattatggg cattcttagc tatccagcgt ccttcctcag ttttaccaaa actagcatat
      301 agattttcac aacaagttgg taaacaatat acctacggta aaggattaga agagctgtca
      361 gaagtagaac aattaaaatt agaaaataag agaaaagata tagaattgga tattttaaaa
      421 aagtacaagg tattggaaag gaagtggtac caacagtagt catagattta gtggatcaat
      481 taaaagtaaa atattcaatc aaattgctac taaaagtatt aaacatacct aaatcaacat
      541 attaccgatg gaaaaacaaa acccataaaa atgataccgt aacacaaaaa gttattgaat
      601 tatgtaaagc taaccactat acctacggtt atcgtaagat tacagcattg attaatcaat
      661 gttatacatc accaattaat cataagagag tacagagaat gatgcagaag catcatttga
      721 actgccgagt tagacctaaa aagatgacaa aaataggtaa accgtattat aaaacggaca
      781 atttattaca aagacaattt aaagcgagtt gtccaatgga agtattaaca accgatatta
      841 cttatttacc atttggtcat tctatgttgt atttatcttc gataatggat atttataacg
      901 gagaaattgt ggcgtataaa atagatgata aacaagacca aagtttagtt aatgatacat
      961 taaatcaaat cgatatacct gagggttgta tattacatag tgatcaaggc agcgtttata
     1021 catcttatgc ttattatcaa ttgtgcgaag aaaaaggcat tatcagaagt atgtcccgaa
     1081 agggaacacc tgccgataac gccccgatag aaagtttcca t
//
LOCUS       DAKTLH010000288         1099 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.297, whole genome shotgun sequence.
ACCESSION   DAKTLH010000288 DAKTLH010000000
VERSION     DAKTLH010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.297"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..378
                     /locus_tag="O0394_002846"
     CDS             <1..378
                     /locus_tag="O0394_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HCY0500847.1"
                     /translation="QDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFIY
                     ELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEYK
                     KEQEREKEKEKQKDDDHSGLDEV"
     gene            463..716
                     /locus_tag="O0394_002847"
                     /pseudo
     CDS             463..716
                     /locus_tag="O0394_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            732..1055
                     /locus_tag="O0394_002848"
     CDS             732..1055
                     /locus_tag="O0394_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500848.1"
                     /translation="MNNEELEMRLLLMKQSIEQLQEELAPNLKTRDLVLLRYMYSYKE
                     INMLDSYLFQLATNKEQITKKQFKTKLENIREVPEIPIRQVNDILEGYKNSELYVELI
                     NSILK"
BASE COUNT          495 a          117 c          192 g          295 t
ORIGIN      
        1 caagatgata aaaatacagt aaataaatta agagatggtt atcttttaag tgataaaaaa
       61 tataaagatt ggacagaaca taaccaagat caaattaaaa aaggcaaaac tgcacaagcc
      121 atgtttatct atgagttaag aggtgatgga aatattaatt taaatgtcca taaatactca
      181 gaagataaaa cagttgattc taaatcattc aaatttagta aacttaaaac cgaagatttt
      241 tctcatagag cggaaacaag agaagaagta gaaaagaaag aaaaagaatt tgaagaagag
      301 tacaaaaaag aacaagaacg agagaaagaa aaagaaaagc aaaaagatga cgaccacagt
      361 ggtttagatg aagtataaat ttggtttgtt gattgtctta cattcagtta tttaattttt
      421 agagaaactg acttatttat agcttagcca acatgatact ataaagctat gattaaaaat
      481 aaaaaatctg tgtttaatgg tgttaaagaa atcagtcccc gtgccggaaa atcataaaaa
      541 aaagatggga taaatttgta aatcccgcat tgaataatgc attaaaacaa gaagaggcta
      601 catggggaaa tgtacaaggt caagtttcac aagcattgat gggaactggt attaaagatt
      661 ctactgcaag aagcataggg ttttgggttt cccaagttgg tcaagcatta atataaaaag
      721 agtgtgataa aatgaataat gaagaattag aaatgagatt gttattaatg aaacaatcaa
      781 tagaacaatt acaagaagaa ctagcgccta acttaaaaac tagagattta gtgttattaa
      841 gatatatgta ttcatataaa gaaattaata tgctagattc ttacttgttc caattggcta
      901 caaataaaga gcaaataaca aaaaaacaat ttaaaacaaa attggaaaac attagagaag
      961 taccagaaat acctattaga caagttaatg atatattaga aggttataaa aatagtgagt
     1021 tatatgttga gttgataaat agcatcctaa aataaaaata aacatactaa gattagctat
     1081 gaaggaatct atgacgata
//
LOCUS       DAKTLH010000289         1095 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.298, whole genome shotgun sequence.
ACCESSION   DAKTLH010000289 DAKTLH010000000
VERSION     DAKTLH010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.298"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..329
                     /locus_tag="O0394_002849"
     CDS             <1..329
                     /locus_tag="O0394_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCY0500849.1"
                     /translation="KEKQKAIYYKEQQARITLYLKHNTKEPNTIKSVHFTNSETSPMG
                     SAVIEGYINENKKDNFTAYATPEHNYQFGGAMIESEKLSELLKPANQLKSPDDIKKEL
                     NKKKSH"
     gene            347..766
                     /locus_tag="O0394_002850"
     CDS             347..766
                     /locus_tag="O0394_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCY0500850.1"
                     /translation="MSKKHVFIIIGVILCICTVSTVMHFKMKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKETNTIKSVHFTNLETSPMGSAVIEGYINENKEDDFTAYASPEHNYQ
                     FGGAMIKSEGVDKLLKPAHERKSPEKIKEELDKKEGH"
BASE COUNT          453 a          160 c          165 g          317 t
ORIGIN      
        1 aaaaagaaaa acaaaaagca atatactata aagaacaaca agcgcgcatt acactttatc
       61 tcaagcataa cactaaagaa ccgaatacaa tcaaatctgt acatttcaca aactcggaaa
      121 caagtcctat gggaagtgct gtgattgaag gttacataaa tgaaaacaaa aaagataatt
      181 ttactgctta tgctacacca gaacataatt atcaatttgg tggtgctatg atagaaagtg
      241 aaaaattaag cgagttacta aagccagcca atcagttaaa atcaccagat gatataaaaa
      301 aagaactaaa taaaaagaag agtcactaaa gttaggagtt actttaatgt ccaaaaaaca
      361 tgtttttata attattggtg tcatattgtg tatatgtaca gtttctacgg tcatgcattt
      421 taaaatgaaa tatgatgaaa aagaaaaaca aaaagcgatt tactacaaag aacaacaaga
      481 acgtattaca ctctatctta agcataatac taaagaaacg aacacgatta aatctgtaca
      541 tttcacaaac ttggaaacaa gtcctatggg aagtgctgtg attgaaggat acatcaatga
      601 aaataaagaa gatgatttta ctgcttatgc atcgcctgaa cataattatc aatttggtgg
      661 cgctatgata aaaagtgaag gagtagataa attattaaaa ccagcacatg aaagaaaatc
      721 accagaaaaa atcaaagaag aattagataa aaaagaaggc cactagggtc ttctttattt
      781 ttgatttaat cttccaataa tctatgtcat tgctatcgaa ggtgtttcgc aattaatata
      841 aatcacttca tcatcaccaa tacttcccca gttttgtaca gtacattaac acaaacgaac
      901 cacgttaatt taaatggaat aatagtttgg ccattataag aacaatatat atcgaataac
      961 aatgtcgatg acgtgattta ttgacattat caaattttct caaatgtgtg tgatttttta
     1021 taaaaaaggg tagagcaagg tataatatcc tttcaatctg ttttcatatt ttatatattt
     1081 tttaaatata agtgc
//
LOCUS       DAKTLH010000290         1089 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.299, whole genome shotgun sequence.
ACCESSION   DAKTLH010000290 DAKTLH010000000
VERSION     DAKTLH010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.299"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..759
                     /locus_tag="O0394_002851"
     CDS             <1..759
                     /locus_tag="O0394_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0500851.1"
                     /translation="LVLLIILIVGYVIMTKTNGRNAQIKDTFNQTLKLYPTKNLDDFY
                     DKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDMETRGMVLYINRNTRTTKGYYFISEM
                     TDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPNDKLKKEIENFKFFVQYGNFKDINDYK
                     DGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDIPTKQAPKLLLKGDGDLKGSSVGS
                     RSLEFTFVENKEENIYFTDSVQYTPSEDTRYESN"
     gene            complement(763..885)
                     /locus_tag="O0394_002852"
     CDS             complement(763..885)
                     /locus_tag="O0394_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500852.1"
                     /translation="MSYIIKFTFKFFEMATYISQLTLDIELVSSELLVILPGFI"
BASE COUNT          448 a          145 c          197 g          299 t
ORIGIN      
        1 ttggtattac tcattatatt gattgtaggt tatgtcatta tgacaaaaac aaatggtcga
       61 aacgcccaaa ttaaagacac atttaatcaa acattaaaat tatatccaac caaaaatctc
      121 gatgattttt acgataaaga aggctttcga gatcaagaat ttaaaaaggg tgataaaggt
      181 acttggatag ttaattctga aatggtaatc gagccaaaag gtaaggatat ggaaacgaga
      241 ggaatggtgc tctatatcaa tcgcaatact agaaccacaa aagggtatta ttttataagt
      301 gaaatgacag atgacagtaa cggcagacca aaggatgatg aaaaaaggta tccggtaaaa
      361 atggaacata ataaaatcat accaacgaag ccactaccga atgacaagtt aaaaaaagag
      421 attgaaaact ttaagttctt tgtacaatat ggcaacttta aagatattaa tgattataaa
      481 gatggtgata tttcatataa tcctaatgta ccaagttatt cggcaaaata tcaattgaat
      541 aatgatgatt ataatgtcca acagttaaga aaaagatatg atattccaac caaacaagcg
      601 ccgaaactat tattgaaagg cgatggagat ttaaaaggtt catccgtagg ttctagaagt
      661 cttgaattta cctttgtcga aaataaagaa gaaaacattt attttacaga ttctgtacaa
      721 tatactccaa gtgaggatac aaggtatgag tcaaactgaa tatcaaataa aacctggtaa
      781 tataacaagt aactctgaag aaacaagttc gatatctaaa gtgagctgtg aaatataggt
      841 agccatttca aaaaatttaa aggtgaattt gataatgtag ctcaaggaga ttgggttaaa
      901 aaggcgaaga atgaactgga tgatattagt aagaaattaa aaaatattca aagaacggaa
      961 gtttaatagc ttatatgatt cttggagcta agacagcatg cgttcattca tgccattatt
     1021 aatataagca ccgcaacaaa aaagcttcta atgtgataca ggaacctcat attccgtatc
     1081 atgttagaa
//
LOCUS       DAKTLH010000291         1061 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.300, whole genome shotgun sequence.
ACCESSION   DAKTLH010000291 DAKTLH010000000
VERSION     DAKTLH010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.300"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(172..>1061)
                     /gene="sasG"
                     /locus_tag="O0394_002853"
     CDS             complement(172..>1061)
                     /gene="sasG"
                     /locus_tag="O0394_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
                     /protein_id="HCY0500853.1"
                     /translation="KERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKG
                     ESKEEITKDPVNELTEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTGKVIE
                     EPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPIT
                     VNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSGPVNP
                     NNPGLSKDRAKPNGPVHSMDKNDKVKKSKIAKESVANQEKKRAELPKTGLESTQKGLI
                     FSSIIGIAGLMLLARRRKN"
BASE COUNT          225 a          206 c          169 g          461 t
ORIGIN      
        1 atatgaaaat attatcaaaa aaatcaaatt tataacaaaa atacacccct taaagttagg
       61 tctttcaatc caacttttgg ggtgtatatc attctcatca tattctaggt tgtttttaac
      121 aaactaaata tagtgaatgc aaatcaacta ttatttaaat tatgaattat tttaattctt
      181 tcttctacga gccaataaca ttaatccagc aattccaatt atactactaa agatcaaacc
      241 tttttgcgtg ctttctaaac ctgtttttgg taattctgct cgttttttct cttgattagc
      301 tactgattct ttagcaattt tagatttttt aactttatca tttttatcca ttgaatgaac
      361 tgggccattt ggttttgctc tgtctttcga taatcctgga ttgttaggat ttactgggcc
      421 acttggatga gttggtctgc tcggcttctc tgggttttca ggtccttttg gatcttttgg
      481 tttctctcca ccgaactcta caatcttatc tactggttgt tttgtgatct cttctgttgg
      541 ttgaccctcg ccaacttttt cacctgttaa tgggttcact gtgattggtg ttgtgattgt
      601 cttacttcct ggttgtcctt cttgtttcac tcgctcttca ccaggttgta attttggatt
      661 aaactcacgt tttgtttcaa acggtatctc tactgttttt gtttctggtg tacccgtttt
      721 tggtccgtgt ttaatcacat catccactgg ctcttcgatc acttttcctg tgtctggatt
      781 cttgattcct ggtttacctg gtactttttc cgtttgatct gtgggtaagt ttggatcaaa
      841 gatatcttta tgaccttgcg gtattttctc gccaccgaat tctgttaatt cattaactgg
      901 atcttttgtg atttcttctt tcgattcacc tttactaata atttctccag ttaatggatt
      961 ttttagtgtt ggcgtcgtta ttgtcttctc acctttttgt ccttctcttg ttactttttc
     1021 tgtccctggt gctaaatcag gattaaattt acgttctttc t
//
LOCUS       DAKTLH010000292         1057 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.301, whole genome shotgun sequence.
ACCESSION   DAKTLH010000292 DAKTLH010000000
VERSION     DAKTLH010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.301"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(61..180)
                     /locus_tag="O0394_002854"
     CDS             complement(61..180)
                     /locus_tag="O0394_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500854.1"
                     /translation="MGGCPSVTMDACALLQKFDFCNNISHFRHFFAIKQPIER"
     gene            complement(260..400)
                     /locus_tag="O0394_002855"
     CDS             complement(260..400)
                     /locus_tag="O0394_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500855.1"
                     /translation="MSKAYRLYNNIQDDTKQPMAHAVGCFFRCVCHGQHFDVGIPLQA
                     WE"
     gene            complement(404..1003)
                     /locus_tag="O0394_002856"
     CDS             complement(404..1003)
                     /locus_tag="O0394_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HCY0500856.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQARTEEIKYYLAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
BASE COUNT          297 a          209 c          138 g          413 t
ORIGIN      
        1 cattattatt ttatctttgg caactaaata gataactcat attttttaaa agcgaattta
       61 ttatcgctca attggttgtt ttatagcaaa aaagtgtcga aaatgtgaaa tattattaca
      121 aaagtcgaat ttttgtaata atgcacacgc atccattgtt acagacggac aaccgcccat
      181 ccgccactca caaagtcatc cttatatttt aatatcttca tcattcattg tagacaccct
      241 tgccttttgc taacattttc tactcccaag cctgtaacgg aattccaacg tcaaagtgtt
      301 gcccatgaca gacacaccta aaaaaacaac caacagcatg agccattggt tgtttcgtgt
      361 catcttgtat gttgttgtat aaccggtatg ctttactcat taattactta tcatgcatcc
      421 ctttaccttc tagtatttgg ttaatatact tttcaacacg cttttgtctt gtttcactgc
      481 gtttagcttg tccaatatga tatatatatt gatgttgtct gcctggcgtt aatttataaa
      541 atgaagactc taactgtggc atttcttcga acttagcttc taactcttta ggaataacat
      601 attcctctgt tttcttcatt tcaacttttt taccagcttt ttcagcttta attgcttcgg
      661 ctagataata tttaatttct tcggtacgtg cttgaatctc tgttaaattt tcaaatcgta
      721 actgacgagc tgcttgcacc ttttcagtct gttgaatgag tgtatgatat ttatcctcca
      781 aaatggcacc tttatgaaat aatagtgcaa cataattttt gaagccatgg ataagtacta
      841 catttttatt attcaacgtg taacaaggat gcatccattt atacgcttcc tctaattctg
      901 tttcattaaa gattaacgct ctcaaatatt tatattcttc ttgccattga ctctctttat
      961 ttaaaaatgc ttcaacttgt tcatttgatt gtttctcact catagtcatc acttccattt
     1021 tgtttttcaa tacacattgt attatatatc ctaattt
//
LOCUS       DAKTLH010000293         1046 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.302, whole genome shotgun sequence.
ACCESSION   DAKTLH010000293 DAKTLH010000000
VERSION     DAKTLH010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.302"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(224..583)
                     /locus_tag="O0394_002857"
     CDS             complement(224..583)
                     /locus_tag="O0394_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HCY0500857.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(596..832)
                     /locus_tag="O0394_002858"
     CDS             complement(596..832)
                     /locus_tag="O0394_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HCY0500858.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
BASE COUNT          342 a          200 c          127 g          377 t
ORIGIN      
        1 aaaaattaat taattttttc gtagcctgat ttcgattaaa aatgtatccg ctatcacgat
       61 catttaaaat actatttata gtcagattct taattttcaa taagcttttt aataaaacaa
      121 aatatcttta cgcaaaaata ggacataatc aaatatgtcc ttaatcgtta ttgatatcaa
      181 tttgtatccc aaaaattggg ttaaccatta aaaagttatt ttattagcaa ttttcaggat
      241 tacctgcaac ttttgcagtt ctaaatgaac taccacagcc acatgaagca attgcattag
      301 gattgtcgat ttggaaaccg ccacccatta atgattgctt aaaatcaata gtcgtaccat
      361 ttaatacggg tgcatctttt ttgtctacta atacttttaa tccaaagtat tctaagactt
      421 catcattttc accaggcgct tcttctgcac ccataccgta tgttaaacca gtgcacccgc
      481 caccattcac tttaattttt aaatagccat ctggcatttc atttgcttta agcatatctt
      541 ttacttcgta agcagctgct tctgttaata taactgttgg catgataact cctccttaaa
      601 aaatccaagt ttcttttata tgtgcatata tattttgtaa taattcttcc ggcgaatcac
      661 cttcaacaat atcaccattt actaaagcat acaacccggc tgaacatata ccacaatgtg
      721 tcaggcaacc atactctaac acatcgacat ctgggtcatt ttccagttga ttaaaaacat
      781 aatctccacc ttttgccatg ttagagagac aaaattctac gatcggattc atacttcacc
      841 ttcttatttc atttgttaca atattatagc attttaaaac tggtatttta acatgatgtg
      901 ctcaattagc aacaactgat gtttcttatc ccagttatgt aatagtgcct tagttagtac
      961 aaatatttat ttgtagtcat tttcaaaaaa atatataata agcatataaa tacatattta
     1021 cttttttagg ggttattgat gaatca
//
LOCUS       DAKTLH010000294         1018 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.303, whole genome shotgun sequence.
ACCESSION   DAKTLH010000294 DAKTLH010000000
VERSION     DAKTLH010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.303"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..397)
                     /locus_tag="O0394_002859"
     CDS             complement(<1..397)
                     /locus_tag="O0394_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500859.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGNFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAV"
     gene            549..683
                     /gene="graF"
                     /locus_tag="O0394_002860"
     CDS             549..683
                     /gene="graF"
                     /locus_tag="O0394_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HCY0500860.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
BASE COUNT          402 a          157 c          132 g          327 t
ORIGIN      
        1 tcactgcaaa gaatccataa acttgagtca ttaatataag agtaataata aagtacatga
       61 taccgataac taagaaaaag atagaaatat catagtcata cttaatagtt ctaagtaatg
      121 tatgaccaat caacataact aaaataccga atataattat cacaattgca ctaactatag
      181 ggaaattacc tataaatcga ctcattactt taataatttc actgaaaaat gcattcagaa
      241 attcccacac attatgaaca tgtgtatcaa cttttttacc accatttaat tgtttaatta
      301 atggcaagtt aattaaaata atgagaccag tgaagagtga aacgataccg attaaatagc
      361 taaaaataat ttcggcgtgt ttcctaagaa aagacattat gttcacctca aaatcattat
      421 atattaatct aatgttgttg tctttagata aatgaataaa tagtgctata gacttagaaa
      481 agtttaacga aagtacaacc gggtaagatt tatatataaa taattatcca atttgggagg
      541 aattaatcat gtctaatgaa aatcaaaata aaaaagcagc agaaaaagct aaagaagttg
      601 aagaaaaatt aaaagataaa aaagaagaaa aaacagaaga tattaatcaa acaaaacaag
      661 atatccaaga tacactaaat taatttaaat ccccttacaa cttttagttg ttaggggatt
      721 ttatgcatgt gctcggcgca tgtatccctc tccttcctta caacttttag ttgttagggg
      781 cttttatgca tgtgctcggc gcatgtatcc ctcttcttcc ttataaacta taattcgtaa
      841 gccattatgc acgggttctt cactgttgtt aaagaggtat gattgataag tatccctctt
      901 catttatcag ctcaatatta ttacatgtca catattttta tatctttaat tactaacatt
      961 cctactacat cttcaattta aatccaatgc taatattata aattttaaat cgaattaa
//
LOCUS       DAKTLH010000295          987 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.304, whole genome shotgun sequence.
ACCESSION   DAKTLH010000295 DAKTLH010000000
VERSION     DAKTLH010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.304"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..230
                     /locus_tag="O0394_002861"
     CDS             <1..230
                     /locus_tag="O0394_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500861.1"
                     /translation="EMIYESDVNQFSVDLLMPKKQIEALVYNFYEVNNINLSSGLSEK
                     ERNKLLNLISTKLEVSKVAAGLRLYNLGIHI"
     gene            complement(448..>987)
                     /locus_tag="O0394_002862"
     CDS             complement(448..>987)
                     /locus_tag="O0394_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500862.1"
                     /translation="EYIAKYNEYSQLLDATYSQAVAYLLSKYGAVTDDYYKEKSYTRF
                     LNGEIKSISKGKYTRASEGLYCHHISEDKFQNLSDLRFISKFKYSYDVQKKENLVYCD
                     LIEHLILHAIITKESHGQFGVAGLCQMIKPTVIDWYIGEYNPKPAWMQATKARAYLPG
                     ILVEKLLIKIDDMLKGIEI"
BASE COUNT          332 a          148 c          147 g          360 t
ORIGIN      
        1 atgaaatgat ttatgaaagc gacgtaaatc agttttcagt cgacttactt atgcctaaaa
       61 agcaaataga ggcattagtc tataattttt atgaagtcaa taatatcaat ttaagttctg
      121 gtttatctga aaaagaacga aacaaactat tgaaccttat atcaactaag ttggaagttt
      181 cgaaagtagc tgctggatta aggctgtata atttaggaat acatatttga taaatattgt
      241 gaaaatctag tcgatttatt aaaaccaaaa gaaatttaat acaattcaag accatgcaaa
      301 ctcatttata aacattgtcc gtttatagat aggtatgcgt ggtctatttt ataaaatata
      361 tttaattaca ggtttaaaca tctataacaa aatctcaaca gcaaaaaata aatctacatt
      421 aatcatttat ctagactcaa ggaaatctta tatttctatt ccttttaaca tatcgtcaat
      481 tttaatgagt aatttctcta ctaatattcc aggcaaatag gcacgtgctt tggtggcttg
      541 catccatgct ggttttggat tatattcgcc aatgtaccaa tcaatgactg ttggtttgat
      601 catttgacat aatccagcta caccaaattg gccatgggat tcttttgtaa taattgcatg
      661 taaaattaaa tgctcgatta gatcacagta cactaagttt tctttctttt gaacgtcgta
      721 tgagtactta aatttggaaa tgaatcttag atcagataga ttttggaatt tgtcctcgct
      781 tatatgatgg caatataatc cttcgctagc tctagtgtat tttccttttg aaatactttt
      841 gatttctcca tttaaaaatc gcgtgtatga tttttcttta taataatcat cggttacagc
      901 gccatattta cttaaaaggt atgctacagc ttgcgagtat gtagcgtcta ataattgact
      961 atattcatta tatttagcta tatattc
//
LOCUS       DAKTLH010000296          970 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.305, whole genome shotgun sequence.
ACCESSION   DAKTLH010000296 DAKTLH010000000
VERSION     DAKTLH010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.305"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            57..491
                     /locus_tag="O0394_002863"
     CDS             57..491
                     /locus_tag="O0394_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCY0500863.1"
                     /translation="MKLKSFITVTLALGMIATTGATVAGNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
     gene            complement(748..933)
                     /locus_tag="O0394_002864"
     CDS             complement(748..933)
                     /locus_tag="O0394_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCY0500864.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
BASE COUNT          366 a          142 c          156 g          306 t
ORIGIN      
        1 aatttgtgtt atattgattt tgtaagatac tttaactcac aaaggagaga gattatatga
       61 aattaaaatc atttataact gtaactttgg cactgggcat gatcgcaacg actggcgcta
      121 ctgtggcagg taatgaggta tctgcagcag aaaaggacaa actaccggca actcaaaaag
      181 ctaaagaaat gcaaaatgtt ccatatacaa ttgcagtaga tggcattatg gctttcaatc
      241 aatcttactt aaatttacca aaagatagcc aattatcata tttagattta ggaaataaag
      301 ttaaagcttt gttatatgat gaacgcggtg taacacctga gaagattcga aatgcaaaat
      361 ctgccgttta cacgattact tggaaagatg gtagtaaaaa agaagtggat cttaagaaag
      421 atagctacac agcaaacttg tttgattcaa attcaattaa acaaattgat attaatgtaa
      481 aaactaaata aacacttaaa attatcaaat tcaacatgaa gcatttaatt ttacagtgat
      541 gattataaaa taattgcctt gatacaaaga ttactcgtaa atgacatctt tgtattaagg
      601 ctttttctaa atttaaaagt gatgggttag aggtcattga gctttaaaat attcaaaata
      661 caaaacatta atggccaaaa ataaaagccg cctttatctg ggcagcttca ataataagaa
      721 agacatattt cattttatac taaatagtta ttgtgatgaa tctttcggcg gtttaattac
      781 tgcagcaaaa attgctgtga aaatcgtgaa caatactgcc atgataattg gattcactac
      841 atttaagctg tctccaccta ctaggctatt aagtacaaag ttaaccattt gcattaataa
      901 taatgcccaa aagaatgtta cgaggtgttt catgtcattc tacctccact ttaattatat
      961 atattttatt
//
LOCUS       DAKTLH010000297          962 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.306, whole genome shotgun sequence.
ACCESSION   DAKTLH010000297 DAKTLH010000000
VERSION     DAKTLH010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.306"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(763..>962)
                     /locus_tag="O0394_002865"
     CDS             complement(763..>962)
                     /locus_tag="O0394_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCY0500865.1"
                     /translation="FVTGNAPAKLTHANLCNINLKIPCLTEQDKVSALLKSIDNKMNN
                     QMNRIELLKERKKGLLQKMFI"
BASE COUNT          294 a          132 c          121 g          415 t
ORIGIN      
        1 tatgccacta cagtgtcaac attttataaa cactattcat ttgaacatgg tctaaaacga
       61 ataggacatt gtaaatgatt aaatagtctg tctctagttg atgtatatgg tcatttgtta
      121 tgcagataat aattaatcta attaattttt acgttttagt tacttggttg tgattgaggc
      181 tttttgtatc tagttgtaat attctccttt tttagaatca taataggtac ttcttgaaat
      241 ttttagaact ttgcacattg ctgatactga atattgatac gcattttgtt gaaagacttc
      301 attttttgtc tcaaaattag cactgcttgc tttaaatcat cattttacat tatcaatcga
      361 tgtttttctt tgcgtaattt tattaactct ttttcatcac ttgttaaaat gtcttgatga
      421 ttgaatgact cagtattttg gttttgtttt atctaatttg agaaagttaa tgatgttaga
      481 tcatattctc gtataatttc gtttctaagc ttaccatttt cataaagttt aattatttta
      541 atttaaatta tttactaaaa gctcttcagt ctcttgtcat aataaaatcg cctaccctct
      601 tagtttaaca atgtctattc tcatagaatt tgtccaacta aatgtagacg attcactaaa
      661 cttctaaaaa aataaacccc aaattataat agtctcaata ttaatatatt acaatttatt
      721 cataggattt ttatcatttc aattaatagg ttgttatcag ggttaaataa acattttttg
      781 taatagtcct tttttacgtt cttttaataa ctcaattcta ttcatttgat tattcatttt
      841 attgtctata gattttaaca atgcacttac tttatcttgt tcagtgagac aaggtatttt
      901 aagatttata ttgcataagt tcgcatgagt taatttagct ggtgcattac ctgtgacaaa
      961 tg
//
LOCUS       DAKTLH010000298          961 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.307, whole genome shotgun sequence.
ACCESSION   DAKTLH010000298 DAKTLH010000000
VERSION     DAKTLH010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.307"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..>961
                     /locus_tag="O0394_002866"
     CDS             <1..>961
                     /locus_tag="O0394_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCY0500866.1"
                     /translation="SNINLEKIRPPYKSNWYKKEYSNLNIDDYIKSLKNKLDSHLHFS
                     IDKIKNSMTTEYGRGTGAYGDFGRYVFGYAVRNWVKGFKSDQDLSNIALMRIFEMGYD
                     AKLHGEFDMWVNRYDNFNNSIERISKKYQWIAYYEILAKLVDKFPDVQYSGLWDDYIR
                     DIDPTLLLLEIDKESKILVPSPLPSHQSNEWVKNTKVFDETKLFLEIDIDNHRYICLS
                     SKFNFEKREKEIPFEDRDSCYFLAMGYFYNKEDSNEIIKGYENNYDRGINIPRAHSIY
                     LYEYYWSEAYKNYKEGYLTESDGKLCPAIYEYFWELDYSVKDKS"
BASE COUNT          383 a           94 c          163 g          321 t
ORIGIN      
        1 atctaatata aatttagaaa aaattaggcc accttataaa agtaattggt ataaaaaaga
       61 atattcgaat ttaaatatag acgattacat aaaatcttta aaaaacaaat tagatagcca
      121 tttacatttt tctatcgata aaattaaaaa ctcaatgaca acggaatatg gaagaggaac
      181 tggggcatat ggcgattttg gcagatatgt atttggttat gctgtacgaa actgggtaaa
      241 gggttttaaa agtgatcaag acttaagtaa tatagcatta atgaggattt ttgaaatggg
      301 ttatgatgca aaattacatg gtgaatttga tatgtgggtt aatcgttatg ataactttaa
      361 taattcaatt gaaagaatta gcaaaaaata tcaatggatt gcttactatg aaattttagc
      421 taaacttgta gataaatttc ctgatgttca atattctgga ctatgggatg attatattag
      481 agatattgat cctactttac tattacttga aattgataaa gaatcaaaaa tactagtacc
      541 ctcaccactt ccttcgcatc aatcgaatga atgggtaaaa aataccaaag tttttgatga
      601 aacaaaatta tttttagaaa tagatataga caatcataga tatatatgtt taagctcaaa
      661 gtttaatttt gaaaagagag agaaagaaat accatttgaa gatagagact catgttattt
      721 cttagcgatg ggatattttt ataataaaga agatagtaat gaaattatca aaggttatga
      781 aaataattat gatagaggta ttaatattcc tcgagcacat agtatatatt tatatgaata
      841 ttattggagc gaagcttata aaaattataa agagggatat ttaactgaat cagatggtaa
      901 attatgtcca gctatttatg aatatttttg ggagttagat tattctgtta aggacaaatc
      961 a
//
LOCUS       DAKTLH010000299          937 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.308, whole genome shotgun sequence.
ACCESSION   DAKTLH010000299 DAKTLH010000000
VERSION     DAKTLH010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.308"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..>937
                     /locus_tag="O0394_002867"
     rRNA            <1..>937
                     /locus_tag="O0394_002867"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          249 a          217 c          278 g          193 t
ORIGIN      
        1 cactgccgag aaaagcctct agatagaaaa taggtgcccg taccgcaaac cgacacaggt
       61 agtcaagatg agaattctaa ggtgagcgag cgaactctcg ttaaggaact cggcaaaatg
      121 accccgtaac ttcgggagaa ggggtgctct ttagggttaa cgcccagaag agccgcagtg
      181 aataggccca agcgactgtt tatcaaaaac acaggtctct gctaaaccgt aaggtgatgt
      241 ataggggctg acgcctgccc ggtgctggaa ggttaagagg agtggttagc ttctgcgaag
      301 ctacgaatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
      361 ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa cgatttgggc actgtctcaa
      421 cgagagactc ggtgaaatca tagtacctgt gaagatgcag gttacccgcg acaggacgga
      481 aagaccccgt ggagctttac tgtagcctga tattgaaatt cggcacagct tgtacaggat
      541 aggtaggagc ctttgaaacg tgagcgctag cttacgtgga ggcgctggtg ggatactacc
      601 ctagctgtgt tggctttcta acccgcacca cttatcgtgg tgggagacag tgtcaggcgg
      661 gcagtttgac tggggcggtc gcctcctaaa aggtaacgga ggcgctcaaa ggttccctca
      721 gaatggttgg aaatcattca tagagtgtaa aggcataagg gagcttgact gcgagaccta
      781 caagtcgagc agggtcgaaa gacggactta gtgatccggt ggttccgcat ggaagggcca
      841 tcgctcaacg gataaaagct accccgggga taacaggctt atctccccca agagttcaca
      901 tcgacgggga ggtttggcac ctcgatgtcg gctcatc
//
LOCUS       DAKTLH010000300          887 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.309, whole genome shotgun sequence.
ACCESSION   DAKTLH010000300 DAKTLH010000000
VERSION     DAKTLH010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.309"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            42..728
                     /locus_tag="O0394_002868"
     CDS             42..728
                     /locus_tag="O0394_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCY0500867.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFYFATSCL
                     VLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
BASE COUNT          348 a          109 c          168 g          262 t
ORIGIN      
        1 gtttaatatt aaaattaact aaaaagaaag aggtgttagt tatgacagaa tacttattaa
       61 gtgctggcat atgtatggca atcgtttcaa tattacttat agggatggct atcagtaatg
      121 tttcgaaagg gcaatacgca aagaggtttt tctatttcgc tactagttgt ttagtgttaa
      181 ctttagttgt agtttcaagt ctaagtagct cagcaaatgc atcacaaaca gataatggcg
      241 taaatagaag tggttctgaa gatccaacag tatatagtgc aacttcaact aaaaaattac
      301 ataaagaacc tgcgacatta attaaagcga ttgatggtga tacggttaaa ttaatgtaca
      361 aaggtcaacc aatgacattc agactattat tggttgatac acctgaaaca aagcatccta
      421 aaaaaggtgt agagaaatat ggtcctgaag caagtgcatt tacgaaaaaa atggtagaaa
      481 atgcaaagaa aattgaagtc gagtttgaca aaggtcaaag aactgataaa tatggacgtg
      541 gcttagcgta tatttatgct gatggaaaaa tggtaaacga agctttagtt cgtcaaggct
      601 tggctaaagt tgcttatgtt tataaaccta acaatacaca tgaacaactt ttaagaaaaa
      661 gtgaagcaca agcaaaaaaa gagaaattaa atatttggag cgaagacaac gctgattcag
      721 gtcaataatg ctcattgtaa aagtgtcact gctgctagtg gcacttttat aatttttaga
      781 tcacgatatg atttattatc aattcagaat taaaaaagta aatagtatca aaagtaagtg
      841 tatttaatat tagaaaataa aaattttaaa tttagtatta aaatgga
//
LOCUS       DAKTLH010000301          879 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.310, whole genome shotgun sequence.
ACCESSION   DAKTLH010000301 DAKTLH010000000
VERSION     DAKTLH010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.310"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(219..427)
                     /locus_tag="O0394_002869"
                     /pseudo
     CDS             complement(219..427)
                     /locus_tag="O0394_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          293 a          116 c          132 g          338 t
ORIGIN      
        1 aatattatat atacgcttga aaaaataaaa taaaaatatt ataatataat caatcggcat
       61 ctacgtgcgg attataaagt aaagcgtagc ataaccagga gttagtcgat ttttaataaa
      121 ttcgactaaa ttattaataa aggctaaagt aattagttta ctttagcctt tattgcattt
      181 gtaatttata gaactgcgaa caggtgacta ctgttaattt atgtgattaa agtttcaatg
      241 atatttgatt tattagacca tttcaaaatc gcaaggttct catgttctgc tacagtttgc
      301 ggtctttgga agatatatca tttactatta ttaacatgat atattctaat ttgtttgtgg
      361 atgggctgtt tggtttgata tcgcggtaaa tataagcatc attagttata ttgttgaaat
      421 ctaagttgaa tttattatca tacagcaatg tgccaattag ggacattttt aattttgaac
      481 tctacatttt gttgagagta tctggttata tttgcaaaat ggtatgaaca tttatcatca
      541 cttattaagt tgtcttgatg attgaatgac ccagtattta ttcagttcga gaagattgag
      601 agtgttaaat cgtattcgag tataatttcg attctagtca taccattttc ataaagttta
      661 gccatttgta acttaaacta tgaactaaat aatcttctga ctcttgtcaa aataaaatcg
      721 cctacccaaa tagtttaaca atatctattc tcatagaatt tgttcaacta agtgtagacg
      781 attctgcatt atcaataagg ctattcaatt tgctatctta agctgtttcg aaattttcaa
      841 attcgtttca tttaatataa acattgatat tatcacgtc
//
LOCUS       DAKTLH010000302          872 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.311, whole genome shotgun sequence.
ACCESSION   DAKTLH010000302 DAKTLH010000000
VERSION     DAKTLH010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.311"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..649)
                     /locus_tag="O0394_002870"
     CDS             complement(<1..649)
                     /locus_tag="O0394_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500868.1"
                     /translation="MNKLILGIYLYRIFSRAYFYLPFLLIYFLIQGYSIIQLEILMAS
                     YGIAAFLFSLYKEKCFKICNLKDSNKLVVSEIFKIIGLLLLLYQNQYLILVVAQILLG
                     LSYSMMAGVDTAIIKRNITNEKYVQNKSNSYMFLSLLISGIIGSYLYGINIKWPIIMT
                     GIFSILTIIIIRCTLVENRELNLIGETKGKIKKFLPEEKFWILHYSFLRALILGFF"
     gene            complement(642..>872)
                     /locus_tag="O0394_002871"
     CDS             complement(642..>872)
                     /locus_tag="O0394_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HCY0500869.1"
                     /translation="SANISGTADDILITENEAIKHMGENVRYMLRSQNKTNYKTSSTI
                     IKVTDNKIELLREGDIKFEEIKERLGTGIIYE"
BASE COUNT          307 a          137 c           85 g          343 t
ORIGIN      
        1 taaaaaatcc taatattaac gctcttaaaa aagaataatg caatatccaa aacttctctt
       61 ctggtagaaa tttctttatc tttccctttg tttctcctat taaatttaat tccctatttt
      121 caactaatgt gcatcgaata attataattg ttagaattga aaatatacca gtcattatta
      181 taggccattt aatatttatt ccataaagat aactacctat aatccctgaa attaataatg
      241 ataggaacat atagctattt gacttatttt gtacgtattt ctcatttgtt atatttcttt
      301 taattattgc ggtatcaaca cccgccatca ttgagtaact taaccctaat aatatttgtg
      361 ccactactaa aattaaatat tgattttgat ataataacaa caataaaccg atgattttga
      421 atatttcact aacaactaat ttattagaat cttttaagtt acaaatttta aaacacttct
      481 ctttgtatag agagaataaa aatgctgcaa tgccataaga cgccattaat atttctaatt
      541 gtattatgga ataaccttga atcaaaaagt aaattaataa aaacggtaaa taaaagtatg
      601 ctcgtgaaaa aattcggtat aaataaatcc caagtattaa cttattcata aataatacct
      661 gtacctagtc tttcttttat ttcttcaaac tttatatctc cttctcttaa taattcaatt
      721 ttattatctg tcactttaat aatcgtacta gatgttttat agttagtttt attttgactt
      781 cttagcatat atcttacatt ttcgcccatg tgcttaattg cttcattttc agttattaaa
      841 atatcatcgg cagttccaga tatatttgct ga
//
LOCUS       DAKTLH010000303          853 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.312, whole genome shotgun sequence.
ACCESSION   DAKTLH010000303 DAKTLH010000000
VERSION     DAKTLH010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.312"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            169..831
                     /locus_tag="O0394_002872"
                     /pseudo
     CDS             169..831
                     /locus_tag="O0394_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463904.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
BASE COUNT          349 a           96 c          144 g          264 t
ORIGIN      
        1 tttatgttat atcgtaataa gtctttttct gtagtaagag actttatcga gaagcaagaa
       61 tttcccgaaa attataaaat agcatggtat taaagcatcc tttctacaca gataaagaga
      121 aagcgatgct atttttatat gcttttttca ttattcgttg aaaaagaaga gtcacaaaag
      181 ttaacatatt taaaagaact tggtgaagat ggcgaatata aatatgttgc caaaatagat
      241 agtaaaacat ctaaattatg tcattcactc aacggaaaaa tatttaaagt taaagatatg
      301 ataccaggtg tgaatgcgcc acctatgcat ccttggtgta gaagtaccac agtgccacat
      361 gtcggcaatt ggcgagacaa gttctttaaa gagcgtgaag gtaaatatca agtagaaggt
      421 tgttttattg aatcaggtgc acttaataat aaaagtgatg aatacggtat caaaaggaac
      481 agacatgctc aaatttatta taattcagtt agaaatcgag ataaacagat agagatttct
      541 aaaattgcta aaaatactaa tataaataaa aatttaattc aaagagttta tgaacatata
      601 tttgaaaata agtatttatt agaaagtgga tttaaacaat ttgatcctga cttttatatg
      661 gctcaaagtt ggcaacgttt aagagaaggg aaaaatatta aaaaaatgga tataataatg
      721 tagaaacatg aagctcttga acactatcta atgaataagt ataatttaca ctatatagag
      781 gcacataaac ttacagaaat aaaatataat tatagtattt taattaatta gaggtggaaa
      841 tgattgctaa tat
//
LOCUS       DAKTLH010000304          837 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.313, whole genome shotgun sequence.
ACCESSION   DAKTLH010000304 DAKTLH010000000
VERSION     DAKTLH010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.313"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(225..837)
                     /locus_tag="O0394_002873"
                     /pseudo
     CDS             complement(225..837)
                     /locus_tag="O0394_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          281 a          134 c          105 g          317 t
ORIGIN      
        1 catataactc tcatgaaaat tgattaaact aaattaatta gttagcctta aatttaacaa
       61 attaaatcaa accgaatgag gctaaattgg ttatttttca agatatgatt tgtataaatc
      121 aaaacatttc ttacaaatca aatgtgtata aaatgtgata tacattttca accttaaaaa
      181 agttctaaaa aaagatgagc atctactgtc tcatctttca gttctttttt acgggtctgt
      241 tttctaattt gagcacaatc ttcgatttct ttatctaaat gactaccaat taaatctatt
      301 tcttctattg ttaaatcgct atctccatct tcttttatct ctggtattat tttttcttca
      361 actaagtcac gatatagtgt ttttgaattc tcgttcaatt tcgattcgtg attttgaata
      421 ctttcttccg caccaatgta tatctattgg cactagcttc tacttttgta ccatcaataa
      481 aaattgaatt atcatcaata agattttgct ttaaacattg actatagaac tgaataaata
      541 aagattcaat taacgcatca gtattaggat tcactctaaa acgattaata gttttataag
      601 aaagtgtttg atcttgggct aaccacatca ttcgaatact gtcatgaagt aatttctcta
      661 ttctacgacc agaaaataca gattgagtat atgcatataa gatgattttt aacatcattt
      721 ttggatgata ggatgttgca ccacgatgat gtctgaattc atcgaattcg ctatcaggta
      781 tcgtttcaac aatttcatta acatatcgcg aaatatcatt ttgaggaatt ctaacga
//
LOCUS       DAKTLH010000305          835 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.314, whole genome shotgun sequence.
ACCESSION   DAKTLH010000305 DAKTLH010000000
VERSION     DAKTLH010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.314"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..667
                     /locus_tag="O0394_002874"
     CDS             <1..667
                     /locus_tag="O0394_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCY0500870.1"
                     /translation="ISTIQSPIVKGLSMWLAILCLSLLLTFICYLVWTVSYRHRSYNK
                     EVDIIIVLGAGIFTEFVTPMLAARLDRALDIYQQQASATKIIVSGGQGPDEPISEALA
                     MQRYLIAHGVDKTDILMESHSINTYTNFLYSKQIINNLYHEAVKIVCVTSQFHVLRAL
                     RLAQKLGLSIDGVGSHTPYHFFLHVLIKDYLGVMYQYRLLLTLYCSSSWFICLYAAFI
                     YNS"
BASE COUNT          275 a          137 c          131 g          292 t
ORIGIN      
        1 tataagtacg attcagtcac ctattgtgaa aggtttgtct atgtggttag ctatattatg
       61 tctttccctg ttactaacct ttatttgtta tctcgtttgg acagtatcat atagacatag
      121 atcatacaac aaagaagttg atatcataat tgtgttaggt gctggtattt ttaccgaatt
      181 tgtcacacca atgcttgcag cgcgtttaga tcgtgcactt gatatctatc aacaacaagc
      241 atcagcaaca aagatcattg tatctggcgg acaaggacca gacgaaccaa tttctgaagc
      301 tttagctatg caacgttatt tgattgcaca tggtgttgat aaaacagata tactaatgga
      361 atctcactct ataaatacat acacaaactt tttatattct aaacaaatca tcaacaattt
      421 gtatcatgaa gcagttaaaa ttgtttgtgt aacaagtcag tttcatgtat tgcgcgcctt
      481 acgactagcc caaaaattag gtttatctat cgatggtgtc ggaagtcata caccatatca
      541 ctttttctta catgtgctca tcaaagatta tctaggcgta atgtatcagt atagattgtt
      601 attaacgtta tattgttcaa gtagttggtt tatatgtctt tacgccgctt ttatttataa
      661 tagttaatcc acaaacaaac tattattata ggttgtttct aatattgcgt caaactatct
      721 ttaaatattc gttaatcctt gattagacaa ttgttatatt gaaaataaat aaactaaaga
      781 gtctgggaca ttaatcccta aaaaaaacag cagtaagata attttcaatt agaaa
//
LOCUS       DAKTLH010000306          831 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.315, whole genome shotgun sequence.
ACCESSION   DAKTLH010000306 DAKTLH010000000
VERSION     DAKTLH010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.315"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            59..775
                     /locus_tag="O0394_002875"
     CDS             59..775
                     /locus_tag="O0394_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HCY0500871.1"
                     /translation="MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQD
                     ETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQL
                     KDRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAH
                     KIYQGNKPVLTLKEIDFRAREALIKNKILYNENRNKGKLKITGGGNNYTIDLSKRLHS
                     DLANVYVKNPNKITVDVLFD"
BASE COUNT          316 a          125 c          129 g          261 t
ORIGIN      
        1 gctcacattt aaatttctta ttcctcgtat catgattcct ctgaaaggag atgttctaat
       61 gagtaagaac atcacgaaaa atataatttt aacgacaaca ttattactat taggtactgt
      121 attacctcaa aatcaaaaac cagtatttag tttttactct gaagctaaag cttatagcat
      181 tggtcaagat gaaactaaca tcaatgaatt aattaaatat tacacacagc ctcatttttc
      241 attttcaaat aaatggctat atcaatatga taatggaaac atttatgttg aacttaagag
      301 atattcatgg tcagcacata tatctttatg gggcgctgaa agttggggaa atattaatca
      361 gttaaaagat cgttacgtag atgtgtttgg actaaaagac aaagatactg atcagttatg
      421 gtggtcttat agagagacat ttacaggtgg cgttacacca gccgcaaaac cttctgataa
      481 aacttataat ctttttgtgc aatacaaaga taaactacaa acgattattg gtgcgcataa
      541 aatataccaa ggcaataaac cagtattaac attgaaagaa atcgatttcc gtgcacgaga
      601 agcgttaata aaaaataaaa tattatataa cgaaaatcgt aataaaggta agcttaagat
      661 caccggtggc ggtaataact acactattga tttaagcaaa agattacatt cagatctagc
      721 aaatgtttat gttaaaaatc ctaataaaat aactgttgac gtcctctttg attagtatat
      781 gaaggtgact tatacttcat gcactttaat tccaaatcag attatttaaa t
//
LOCUS       DAKTLH010000307          830 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.316, whole genome shotgun sequence.
ACCESSION   DAKTLH010000307 DAKTLH010000000
VERSION     DAKTLH010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.316"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(284..730)
                     /gene="perR"
                     /locus_tag="O0394_002876"
     CDS             complement(284..730)
                     /gene="perR"
                     /locus_tag="O0394_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HCY0500872.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
BASE COUNT          266 a          125 c          103 g          336 t
ORIGIN      
        1 atgcttttta cttcagtttt acaaacaaac ttatactcac tttatggata gttatttagt
       61 agaccttcgt ttgaatattt ttatgtttat ttataaagga tactagttat tagttaatac
      121 cacttccgta gttagaatat tcttttcttc aatttaaata tttttcagaa taagcataaa
      181 aaaaagttaa acaactttca tgtcgtttaa cttcttaaat attacgctta cttatatgaa
      241 gtaagctaac gcttttttaa tttgtcatac taccaaagtt aaattattta tcttggcatt
      301 ctttacaaac tccataaatt tccattcgat gatgtgttac gtcaaagtca gtcatatgct
      361 gagctaatct ttcaatttca tttaactgtg gatattgaaa atcaacaatc ttaccacatt
      421 gttcacatat aatatgataa tgattatgtg tattaaagtc gaatcgactt gatgagtctc
      481 catatgttaa ttcttttaca attccaatat ctttaaacac tcttaagtta ttatatattg
      541 tcgcaacact tatatttgga aaatcaggtg aaagtgcttg ataaatttca tcagctgttg
      601 gatgagtatg tgaagaaatt aaataacgta atattgcttg tctttgaggt gtaattctta
      661 cgcctgcttg tcgcaatgat gcaattgatt cttctagttc atgttcaatt gattctattt
      721 caacactcat ctatatcacc atctttctta tataataatt attattactt actataaata
      781 taactgtagt attgcgcgcg tgtcaatatt tacatgatga tttcatttaa
//
LOCUS       DAKTLH010000308          827 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.317, whole genome shotgun sequence.
ACCESSION   DAKTLH010000308 DAKTLH010000000
VERSION     DAKTLH010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.317"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            252..452
                     /locus_tag="O0394_002877"
     CDS             252..452
                     /locus_tag="O0394_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500873.1"
                     /translation="MTNPTRTKRCGGDVLYGIDNHFQLLLYISENHCQLETKTFFEYF
                     LRIVNKTHDYAILENKKICISH"
     gene            529..660
                     /locus_tag="O0394_002878"
     CDS             529..660
                     /locus_tag="O0394_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500874.1"
                     /translation="MLLPSSKSHIEMMCEMFKLRSFCENKDNNYSFYNSEKSVYDNN"
BASE COUNT          301 a           97 c          132 g          297 t
ORIGIN      
        1 gatgacatta aatagcatct cctcgtgttg attattttgg ttggctgacc aatatttatt
       61 ctagcacgta gagatgcatt ttttgtgaca atggtagaac cttttctgaa ccatacgcat
      121 agcgtatggt tttcttttta caaaaaacaa aagcagtaaa cctaaagtgt cgtaagggtt
      181 tactgctttt ataaaacgtt gttataacgt atattgtctt ttacgggcat ataaaagggg
      241 aatatttgaa aatgaccaat ccaacaagaa cgaaacgttg tgggggggat gttctatatg
      301 gtattgataa tcattttcaa ctactattat acattagtga gaatcattgt caattagaaa
      361 ctaaaacttt ttttgaatat tttttaagaa tagtaaataa aacgcatgat tacgctattt
      421 tagaaaataa aaaaatttgt atttctcatt agaattagaa tatttaaaag tgatgaggtt
      481 taaacattat attgtttaca tactcctttt gaattcatac attatgaaat gttacttcca
      541 agttcaaaat cgcacattga aatgatgtgt gaaatgttta aactacggtc attttgtgaa
      601 aataaagata ataactattc attttacaat agtgaaaagt cagtatatga caacaattaa
      661 tattgcggta aggccttgtg ttacagtatt ctatatttaa gtactgcaat cagaattaac
      721 agaatgccat taactgatta ttaaatattt gagttaataa ataattaatg attgtagctt
      781 gaaaaattta aaacatggtt attgatttgt gataaaattt aaacgta
//
LOCUS       DAKTLH010000309          822 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.318, whole genome shotgun sequence.
ACCESSION   DAKTLH010000309 DAKTLH010000000
VERSION     DAKTLH010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.318"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            1..822
                     /locus_tag="O0394_002879"
                     /pseudo
     CDS             1..822
                     /locus_tag="O0394_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
BASE COUNT          345 a           88 c          130 g          259 t
ORIGIN      
        1 gatactcttg ttagatgata tgctcgacgt caatatatat atataaattc ttttaaaaat
       61 aatacattag agacatttga tgcgactgat cctgaagttg attacatcac tgaaatagga
      121 gattacttat taaaatatga cgttaattct ataaatgatg tcagaattga agtgatagat
      181 aatttggggc attcaaataa tatgctacta aaaacgatac tacatgcaga agttgaaatg
      241 gaagatggaa agaaatattt attacaaaat gggaaatggg gttattttaa tagagaattt
      301 tttgaccttt tgaatgatca tttgaatgag atagaaatta ggtataacac actaactccc
      361 acaggtttag tatttaaaga aggagaagaa ggatatataa aagaaatagt aggaagattg
      421 ccagaagaat atttaatgtt acataaaaaa ttcataaaac caataaataa gaattttata
      481 gtaaaaggaa atggaataga gttggcagac ttatacaata ttaaaaacaa agagcttttc
      541 acgattaaaa gaggaattaa tacatcttta tctctttata gtctagaaca gaatataata
      601 gcaattaacg ccttaaaata tccagaatca tataattttg aagaattaat agaagctatt
      661 cctgataact cggaaaatat atttaatgat atacaacgga gtacaaattt tagtatagta
      721 tggattttac cgatatcatc tattgataat atgcccataa aagatatggt tcatactagt
      781 aacgtaataa ataaaaactt tcaattaact aatttaggtt ca
//
LOCUS       DAKTLH010000310          819 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.319, whole genome shotgun sequence.
ACCESSION   DAKTLH010000310 DAKTLH010000000
VERSION     DAKTLH010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.319"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..260)
                     /locus_tag="O0394_002880"
     rRNA            complement(<1..260)
                     /locus_tag="O0394_002880"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(565..>819)
                     /locus_tag="O0394_002881"
     rRNA            complement(565..>819)
                     /locus_tag="O0394_002881"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          217 a          197 c          143 g          262 t
ORIGIN      
        1 tttcgctcgc cgctactaag ggaatcgaat tttctttctc ttcctccggg tactaagatg
       61 tttcagttct ccgggtgtgc cttctgatat gctatgtatt cacatatcga taacatgaca
      121 taactcatgc tgggtttccc cattcggaaa tctctggatc aaagcttact tacagctccc
      181 caaagcatat cgtcgttagt aacgtccttc atcggcttct agtgccaagg catccaccgt
      241 gcgcccttaa taacttaatc tatgtttcca tcctacagga aacgcgttat taatcttgtg
      301 agtgttcttt cgaacactag cgattatttc ttatgaattc aagcttattt aaaactcttt
      361 attcactcgg ttttgcttgg taaaatctat attttactta cttatctagt tttcaatgta
      421 caatttcttt ttagtcaagc gctcgcataa gcaatatcac tttaaccaaa aaatatttga
      481 atgttaaata aacattcaaa actgaataca atatgtcaca ttattccgca tcttctgaag
      541 aagatgttcc gaatatatcc ttagaaagga ggtgatccag ccgcaccttc cgatacggct
      601 accttgttac gacttcaccc caatcatttg tcccaccttc gacggctagc tcctaaaagg
      661 ttactccacc ggcttcgggt gttacaaact ctcgtggtgt gacgggcggt gtgtacaaga
      721 cccgggaacg tattcaccgt agcatgctga tctacgatta ctagcgattc cagcttcatg
      781 tagtcgagtt gcagactaca atccgaactg agaacaact
//
LOCUS       DAKTLH010000311          814 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.320, whole genome shotgun sequence.
ACCESSION   DAKTLH010000311 DAKTLH010000000
VERSION     DAKTLH010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.320"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..385)
                     /locus_tag="O0394_002882"
     CDS             complement(<1..385)
                     /locus_tag="O0394_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaG protein"
                     /protein_id="HCY0500875.1"
                     /translation="MIINELKSCKLKFSKQALTFVPIIVTILFILFINWYLNVNLWNG
                     RQISLFTASFNAITSLLISINVYQVINFEENIGHFNHILGKANRLNWLNASMIFTYTI
                     TAICILLASINLLWHSHDMKITLMFI"
     gene            complement(382..>814)
                     /locus_tag="O0394_002883"
     CDS             complement(382..>814)
                     /locus_tag="O0394_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HCY0500876.1"
                     /translation="LVHCFLVFIIAYIGDLIINPHPVNAMLLLVTILLMYVVSLPLIP
                     LNFLLTRYFGVFVSILINLVLSVICVLFLTLKSLFWVLPWGIMQRIPLITLGILPNGL
                     VVNHNSKYFNDLNALYISIIVSIIIFAIVTFLNNKKSWRLK"
BASE COUNT          327 a          124 c          105 g          258 t
ORIGIN      
        1 ctataaacat aagtgttatt ttcatatcat gtgaatgcca caataaatta attgatgcta
       61 atagaataca tatggctgta atagtataag taaaaatcat tgatgcattt aaccaattta
      121 gcctattagc ttttcctaaa atatgattaa agtgaccaat attttcttca aaattgataa
      181 cttgatagac gtttatagaa attaatagcg atgtaattgc attaaaactc gctgtaaaca
      241 aacttatttg tcgaccattc cataaattta cgtttaaata ccaatttata aataatataa
      301 acaatatggt tacaataatg ggtacaaatg tgagcgcttg cttagaaaat ttcaacttac
      361 atgactttaa ttcgttaatt atcattttaa tcgccaactt ttcttattat ttaaaaatgt
      421 tactatcgcg aaaataatga tgctaacaat aatcgaaata tataaggcat tgagatcatt
      481 aaagtatttt gaattatggt ttacaactaa accattaggt agtataccaa gcgtaataag
      541 cgggattctc tgcattatcc cccacggcaa cacccaaaat aaactcttca atgttaaaaa
      601 caagacacaa atgactgata atactaagtt tattaatatt gatacgaaca caccaaagta
      661 tcgagttaat aaaaagttga gcggtatcaa tggtagagat actacataca tcaacaatat
      721 tgtcaccaat aacaacatag cattaaccgg atgtggatta ataattaggt cacctatata
      781 agcaataata aatactaaaa agcaatgtac caaa
//
LOCUS       DAKTLH010000312          807 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.321, whole genome shotgun sequence.
ACCESSION   DAKTLH010000312 DAKTLH010000000
VERSION     DAKTLH010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.321"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            247..339
                     /locus_tag="O0394_002884"
     CDS             247..339
                     /locus_tag="O0394_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500877.1"
                     /translation="MFIFKTTSKSHFHNNVKSLECIKIPINKNR"
BASE COUNT          319 a          108 c           92 g          288 t
ORIGIN      
        1 atgaataaaa aatacatccg aacaaacaaa atctctatat tctaattcga tttacataga
       61 ttatacacta aaaaaatgat tctcaatgca aatgaatgtg tctctcatag tctaaaaact
      121 cgtatgtttc gaattttatg attcattgat aatagtacca gttaaaagaa ctaagattgt
      181 aattatcccc ccttaaaatt caagaatata ttttcttaat ctatcagcaa gaggttatat
      241 ttcaaagtgt ttatttttaa aacaacatct aaaagccatt ttcataataa tgttaaatca
      301 ttagaatgta taaagattcc aattaataaa aatagataaa atgcaataaa gttccaaata
      361 cttattatta ttcatcacaa attacgtgat gccctctaca acactaaatc aaggatgata
      421 tttattttat atacattagc attcattgtt tgcttaccca cattatcctg tttagtcttt
      481 tttataactc tagtcttgaa tgtcatattt acaaagaaaa aaacactaaa atatttaaaa
      541 ctaacactat ttattcactt tgttctttta tagcaccttt tatgtttatt ttcttgtatt
      601 caataaatag tatggagatg ataatttaaa gtagatatat ttgaagtata ctatagagta
      661 aattatattg gggaaatatg taaacgaacc ggttgatttt atagtggatt aacacttcat
      721 agagaatata atcaagaaca aaaacagtca ataggtgtga atttttttaa agacggatct
      781 gttgctacta aaaaaagaag tatacaa
//
LOCUS       DAKTLH010000313          805 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.322, whole genome shotgun sequence.
ACCESSION   DAKTLH010000313 DAKTLH010000000
VERSION     DAKTLH010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.322"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(248..679)
                     /locus_tag="O0394_002885"
     CDS             complement(248..679)
                     /locus_tag="O0394_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCY0500878.1"
                     /translation="MFKNILLPYDFENDFSAIPDYLEKVTDEDSVVVIYHVVTENDLA
                     ISVKYYNKHKEDIIREKEKKLTPFLRELEKRDIQYKIDVDFGHIKDTILEKITSGDIN
                     NGEFDLVIMSNHRVDLNIKHVLGDVTHKIAKRSSVPVLIVK"
BASE COUNT          264 a          125 c           83 g          333 t
ORIGIN      
        1 tatactatag attcaacttt gcaacagaac ctatattttg ctataattat taaattaatt
       61 gcctttgtgt tagtattccc tggattacta acactttggt tagctgttct aagtgataca
      121 ggtgcagcaa tattagttat attaaattca ttacgtttat taaaaaaata aaaaagccta
      181 aatactctct cgatttgtga gaaagtattt aggctttttg aattaataaa tttcttactt
      241 cttatgttta tttaacaatt agtactggga cagaacttct tttagcaatc ttatgtgtaa
      301 catctcctaa aacatgttta atattcaaat cgactctatg attactcata attactaaat
      361 caaattcacc attatttata tctccagaag taattttttc taagattgta tctttaatat
      421 gcccaaaatc tacatctatt ttatattgaa tatctctttt ttctaattca cgtaaaaatg
      481 gagtgagttt tttctctttt tctctaataa tatcttcttt atgcttatta taatacttga
      541 cactaattgc aagatcattt tctgttacaa cgtgataaat tacaacaact gaatcttcat
      601 cggtgacttt ttctaaatag tcagggatag cactaaaatc attttcgaaa tcatagggta
      661 ataatatatt tttgaacatc tacaccagcc tcctattact tattatttag atgtctatta
      721 cttatatata ccctaaatat gtatgcttat tctcatattc aaattattca aatatattcc
      781 ttatcatgta ttagattcta ttttt
//
LOCUS       DAKTLH010000314          783 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.323, whole genome shotgun sequence.
ACCESSION   DAKTLH010000314 DAKTLH010000000
VERSION     DAKTLH010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.323"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..>783)
                     /locus_tag="O0394_002886"
     CDS             complement(<1..>783)
                     /locus_tag="O0394_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HCY0500879.1"
                     /translation="IMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFII
                     TFIAQLASFIVAIPSFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPL
                     NYLVDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIVFIISVYYIFWLLAKRKYILV
                     TFWTIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQP
                     SYSISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIIS"
BASE COUNT          317 a          111 c          112 g          243 t
ORIGIN      
        1 gactaataat atattccact tcaccaatat ggtcattgtg tggatgtgtt aaaattagat
       61 actctaattc atttatccct ctttcattta gcgttggtaa aatatgatat ttagaaattg
      121 aataactagg ttgtttagta tcatcaatca ctttcccacc tgtatcaatc aagacatttt
      181 ggttcttacc gccttcatat aaaatactgt ctccctgccc cacatttaac attgtaattt
      241 tgtgatgtga atttgttgga aacgttatta ataatgtcag aataattata gtccaaaacg
      301 taaccaatat atatttacgt ttagccaata accaaaatat gtaataaaca gaaattataa
      361 atactataaa tatccaatca ttaaacttgg gaacagaaaa atgtgattgc ttgattcttg
      421 tgaataggtc tagtaaccag tcatgaaaat taaaacttag gtcaaccaag taatttagcg
      481 gcgttaatcc cacaataaaa tgacttgtaa taaagaataa aatagatagc ggaaacaata
      541 taatcgaata gtacggtaca aaaatcaaat tagataaaaa tcccacccat tgaagttgat
      601 gaaagcttgg aatggcaacg ataaatgaag ctaattgtgc aataaacgta attatgaata
      661 atgattgtaa ctttgacaat tgctgtaaaa aaggaaaaag tagcataata aaaaatgaaa
      721 tgatgaatga aaattgaaat ccaatatcat aaacaactag tggatttaaa ataaacataa
      781 tta
//
LOCUS       DAKTLH010000315          771 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.324, whole genome shotgun sequence.
ACCESSION   DAKTLH010000315 DAKTLH010000000
VERSION     DAKTLH010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.324"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            521..733
                     /locus_tag="O0394_002887"
     CDS             521..733
                     /locus_tag="O0394_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500880.1"
                     /translation="MNQFKEIYNTIEKLLNDKSISNYRINQDTDVSYGGISELRSGKR
                     KVNNLTLETAEKLYNYQKQLEIMIED"
BASE COUNT          335 a           78 c          116 g          242 t
ORIGIN      
        1 atatatggag ttctttcttt ttatacaaga tgtatatgta taaatggaaa tatttaatac
       61 attacataaa aacctttagg tgcacttaga gggatgttaa tcataaaaaa agagattttg
      121 aaaaatatag gctattatac tttagaatta caaattataa atcttgccag tggatatggg
      181 agttcaacat gtcacttacc ccaatttgtg gaatgttaaa taactataaa gaggaaagga
      241 gaaaaaatga taagaatggt taaagaaaaa gaacgaatag caaaattaaa tttcgaatag
      301 ctgtgacgag cttaataatt caagtactta attttatcaa atcgttcttt taaaaaggta
      361 aagggcaaaa gacctttacc acattaaata gggaggtggt atttatgaca agagaattaa
      421 ggaaaaaatt aactctctat cttaacattg caactttaat attatttatt attaatttaa
      481 ctagaaaaaa ataaattatt ctttcatttt aacaaaaatt atgaaccagt ttaaagaaat
      541 ttataataca atagaaaaat tactaaatga taaatcaata tctaattata gaattaatca
      601 agacactgat gtttcttatg gtggtataag tgaattaaga agcgggaaaa gaaaagtgaa
      661 taatttaact ttagaaacag cggaaaaact ctataattac caaaaacaat tagaaataat
      721 gattgaagat taaaataagg ccatattttt gaaatatcat ggagtgagcc t
//
LOCUS       DAKTLH010000316          753 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.325, whole genome shotgun sequence.
ACCESSION   DAKTLH010000316 DAKTLH010000000
VERSION     DAKTLH010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.325"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            147..251
                     /locus_tag="O0394_002888"
     CDS             147..251
                     /locus_tag="O0394_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500881.1"
                     /translation="MYFKNSSLNYIPTTFGGEPKKKHFPKMESSSSEP"
     gene            complement(242..314)
                     /locus_tag="O0394_002889"
     tRNA            complement(242..314)
                     /locus_tag="O0394_002889"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(279..281),aa:Lys,seq:ttt)
     gene            complement(318..392)
                     /locus_tag="O0394_002890"
     tRNA            complement(318..392)
                     /locus_tag="O0394_002890"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(358..360),aa:Gln,seq:ttg)
     gene            complement(402..485)
                     /locus_tag="O0394_002891"
     tRNA            complement(402..485)
                     /locus_tag="O0394_002891"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(449..451),aa:Tyr,seq:gta)
     gene            complement(515..590)
                     /locus_tag="O0394_002892"
     tRNA            complement(515..590)
                     /locus_tag="O0394_002892"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(555..557),aa:Val,seq:tac)
     gene            complement(598..669)
                     /locus_tag="O0394_002893"
     tRNA            complement(598..669)
                     /locus_tag="O0394_002893"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(634..636),aa:Glu,seq:ttc)
     gene            complement(671..745)
                     /locus_tag="O0394_002894"
     tRNA            complement(671..745)
                     /locus_tag="O0394_002894"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(711..713),aa:Asn,seq:gtt)
BASE COUNT          233 a          173 c          163 g          184 t
ORIGIN      
        1 taatcaagtg aatttctttt ggttacagaa atttcaacaa ctttaaagca cgtataatga
       61 tgattttcag cttgtacaaa ggagaaaaaa agaagacaac caagcccaat aatggactgg
      121 ccgcctaata ataaaagctc taaaagttgt attttaaaaa tagttcttta aattatatac
      181 ccaccacatt tggtggagaa cctaaaaaaa agcacttccc aaaaatggaa agttcaagta
      241 gtgagccata gaggattcga acctctgacc ctctgattaa aagtcagatg ctctaccaac
      301 tgagctaatg gctctaatgg ctgggctagc tggattcgaa ccaacgagtg acggagtcaa
      361 agtccgttgc cttaccgctt ggctatagcc caatatatag atggtggagg ggggcagatt
      421 cgaactgccg aacccgaagg agcggattta cagtccgccg cgtttagcca cttcgctacc
      481 cctccagctt attcatataa tttaataatc aaaatggtgg agaatgacgg gttcgaaccg
      541 ccgaccctct gcttgtaagg cagatgctct cccagctgag ctaattctcc aaaataatga
      601 ctcctacggg actcgaaccc gtgttaccgc cgtgaaaggg cggtgtctta accgcttgac
      661 caaggagcca tggctccaca ggtaggactc gaacctacga ccgatcggtt aacagccgat
      721 agctctacca ctgagctact gtggattaat att
//
LOCUS       DAKTLH010000317          750 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.326, whole genome shotgun sequence.
ACCESSION   DAKTLH010000317 DAKTLH010000000
VERSION     DAKTLH010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.326"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..718)
                     /locus_tag="O0394_002895"
     CDS             complement(<1..718)
                     /locus_tag="O0394_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HCY0500882.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYFMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAV"
BASE COUNT          282 a          113 c          119 g          236 t
ORIGIN      
        1 tcactgcata aagattgaag ataatccaga ttgtactata aatgaagata ggtacatgac
       61 tgagttcttt aagtgcacta ccatcccact gtggactcgg acgctggaaa gtcaatttag
      121 caatcgtcca actagattgt agaacttcgc ctaataatac acctaaaata tattgataac
      181 tcattgtgac aagtagttga atttctacta tattttcatc ttttaatata aaatacaaca
      241 tgatagaaat taaagttata acaacaatgg gtgagccttt tctagatgtt aaaattaaaa
      301 aataaataaa tatcaataaa taggtaaata taaagaaact aggtatctga taatggctcg
      361 acgctaaacc tatcaataac ataataggtg gcataaaata accaccaatc gttgtaagcc
      421 attggcctgc tagatgtcta gattgtgtaa ttgcgaatcc ttgttgtaat gtctgttgtc
      481 gctctcgtgg acttgttaca atgactaaat cttttgcacg gccaccagcg agtttattaa
      541 acagtacatg accaaattca tgtgttaaaa cagggatata gtttaaaatg acatctaaat
      601 agttcaaaac aggcttatgt ctatattgat gaatagcaat ataacaagct gcaacaataa
      661 cgataatgta tatattaagt tgaattgtcg tattaaaaaa gtttgataaa taattcattg
      721 ttaacctcat ataagatatt aatttaaagt
//
LOCUS       DAKTLH010000318          741 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.327, whole genome shotgun sequence.
ACCESSION   DAKTLH010000318 DAKTLH010000000
VERSION     DAKTLH010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.327"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
BASE COUNT          164 a          188 c          115 g          274 t
ORIGIN      
        1 ctgctgttca ctttttataa tacttctgaa tgtcttcact tatacttcta gtcacagatt
       61 taaataatca aaagtgcaca ttattaaaat atcaatttca cactcaatgc ggctcatcgc
      121 attcatttct tgtctagcaa cgttctactc tagcggaacg taagttagct accatcctcg
      181 ctaagaacct ttcttgactt gtgacaatcg cttgcttctt tcctctcctt cggctctcgc
      241 ttactcattt agctctacta aactcgttgc gctcttttct cgtttcgtca gattcaaacg
      301 ttttcacttc gccaagccat ttttctttgt gtttactttt tattttgacg ttttagacat
      361 aaaaaaagag acctcacggt ctcaacttgc ctggcaacgt tctactctag cggaacgtaa
      421 gttggctacc atcgtcgcta aagacctttc ttgacttgtg acaatcgctt gcttctttcc
      481 tcttcttcgg ctctcgctta ctcatttagc tctactaaac tcgttgcgct cttttctcgt
      541 ttcgtcagat tcaaacgttt tcacttcgcc aagccatttt tctttgtgtt tgctttttat
      601 tttgacgttt tagacataaa aaaagagacc ttgcggtctc aatgcggctc atcgcatcca
      661 ttttttgcct ggcaacgttc tactctagcg gaacgtaagt tcgactacca tcgacgctaa
      721 ggagcttaac ttctgtgttc g
//
LOCUS       DAKTLH010000319          729 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.328, whole genome shotgun sequence.
ACCESSION   DAKTLH010000319 DAKTLH010000000
VERSION     DAKTLH010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.328"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            207..458
                     /locus_tag="O0394_002896"
     CDS             207..458
                     /locus_tag="O0394_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCY0500883.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIILIAIVTLILRAMRK"
     gene            complement(497..706)
                     /locus_tag="O0394_002897"
     CDS             complement(497..706)
                     /locus_tag="O0394_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500884.1"
                     /translation="MTTQSFIFMNIILLDGATQRHPFFICQILAFLFPKFPHINLLLF
                     NPILSTFSNIIIHIDHFHANKKDDN"
BASE COUNT          276 a           69 c          144 g          240 t
ORIGIN      
        1 aattcggcag aaaaaataat gtaaatgtat atgatattat cggtacagag actttaaata
       61 acgatagcta cattgaataa aattgatatt caattactac ttttaaaaat atttggataa
      121 aaataatttg aatttgtttt agaattgtaa ataaggggta ctacttagaa taacgcaaaa
      181 taaatagaaa aaggagactg aaaattatgt ttggatttat tggaatgtta attgtcggtg
      241 gcttaattgg atgggctgct ggtgctatta tgggtaaaga tatcccaggt ggtattttag
      301 gcaatattat cgcaggtatt attggatcat gggtaggtgg caaactattc ggacaatggg
      361 gtcctgaatt aggaagtatt tacatcttgc cagcattaat tggttcaatt atcttaattg
      421 caatcgtaac gttaatttta agagctatgc gtaaataata aaaaaattca attggaaatt
      481 gaaataaaga tggcattcag ttgtcatctt ttttatttgc gtgaaaatgg tcaatatgta
      541 ttataatatt tgaaaatgta gataagatag gattaaaaag taataaatta atatgaggaa
      601 attttggaaa taaaaaagcg agtatttgac aaataaaaaa aggatgacgt tgggttgcgc
      661 catccaggag tattatgttc atgaatataa aagactgtgt agtcatttaa gaactacacc
      721 catatagta
//
LOCUS       DAKTLH010000320          728 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.329, whole genome shotgun sequence.
ACCESSION   DAKTLH010000320 DAKTLH010000000
VERSION     DAKTLH010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.329"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..>728)
                     /locus_tag="O0394_002898"
     rRNA            complement(<1..>728)
                     /locus_tag="O0394_002898"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          149 a          221 c          158 g          200 t
ORIGIN      
        1 aaggatgtca agatttggta aggttcttcg cgttgcttcg aattaaacca catgctccac
       61 cgcttgtgcg ggtccccgtc aattcctttg agtttcaacc ttgcggtcgt actccccagg
      121 cggagtgctt aatgcgttag ctgcagcact aaggggcgga aaccccctaa cacttagcac
      181 tcatcgttta cggcgtggac taccagggta tctaatcctg tttgatcccc acgctttcgc
      241 acatcagcgt cagttacaga ccagaaagtc gccttcgcca ctggtgttcc tccatatctc
      301 tgcgcatttc accgctacac atggaattcc actttcctct tctgcactca agttttccag
      361 tttccaatga ccctccacgg ttgagccgtg ggctttcaca tcagacttaa aaaaccgcct
      421 acgcgcgctt tacgcccaat aattccggat aacgcttgcc acctacgtat taccgcggct
      481 gctggcacgt agttagccgt ggctttctga ttaggtaccg tcaagatgtg cacagttact
      541 tacacatatg ttcttcccta ataacagagt tttacgatcc gaagaccttc atcactcacg
      601 cggcgttgct ccgtcaggct ttcgcccatt gcggaagatt ccctactgct gcctcccgta
      661 ggagtctgga ccgtgtctca gttccagtgt ggccgatcac cctctcaggt cggctatgca
      721 tcgttgcc
//
LOCUS       DAKTLH010000321          705 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.330, whole genome shotgun sequence.
ACCESSION   DAKTLH010000321 DAKTLH010000000
VERSION     DAKTLH010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.330"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..525)
                     /locus_tag="O0394_002899"
     CDS             complement(<1..525)
                     /locus_tag="O0394_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCY0500885.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLA"
     regulatory      complement(587..>705)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT          254 a          130 c          114 g          207 t
ORIGIN      
        1 cgcaagcctt ctatatagta aaataaatac aatagaaata acgatacctt taataatatt
       61 gaatggtata attcctgaaa caatgattac tttaagatta tttgcgatat cagctaagtt
      121 aaatatcata ccgtacaaag gtaatagaac gaaatagttc aaaatactca acacgatagt
      181 cataacgatt gttgcaatga ttaatccagt aatcaaagat tttgttgaac gtttattttt
      241 atagatggcg taagcagtta ataagaaact tgcgcctgct aaaaagttag caaatggtcc
      301 aactggatcg cccatactaa ataagtagtt caataaattt ttaaccagtg caactacgat
      361 accggcaact ggtccaaacg taaatgtagc tagtagtgac ggtacatcac taaaatctaa
      421 agttaagtat ggtggcaaaa atggtatagg aaacttgata aaagttaaca caaacgcaat
      481 cgcgctcaac atacttattg tgataagacg tttattttgt tgcatattca attttctcct
      541 tccattcatc tcgtaagaat aggaggatta ttgcacaaca aaaaacctcc atcttctccc
      601 atccagacta tactgtcggc tctagaatct cactagatca gccactaata tgatacatac
      661 tagcaggtcg caggctttat ttactgccgg ttgggaatta caccc
//
LOCUS       DAKTLH010000322          690 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.331, whole genome shotgun sequence.
ACCESSION   DAKTLH010000322 DAKTLH010000000
VERSION     DAKTLH010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.331"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..289)
                     /locus_tag="O0394_002900"
     CDS             complement(<1..289)
                     /locus_tag="O0394_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500886.1"
                     /translation="MFTPIVINLLNDVGSIFLVRVGNQLKVIEKVHYEDILESKIVRL
                     FQAFIKDKMDQLFITSEHFAIDIDKLLFQLNLNVRYIKMEGNTSGYLSGRTI"
     gene            complement(403..588)
                     /locus_tag="O0394_002901"
     CDS             complement(403..588)
                     /locus_tag="O0394_002901"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500887.1"
                     /translation="MKQISIGYEEIYGDRIFHSHFLKEHEVKNWKTVNQIIEKEHLDK
                     NEYEAEPPLFFFSGSLD"
BASE COUNT          254 a          116 c           68 g          252 t
ORIGIN      
        1 agattgttct tccacttaaa taacccgaag tattaccctc cattttaata tatctaacat
       61 ttaaattaag ttgaaataat agcttgtcta tgtcaatagc aaagtgttca gaagtaataa
      121 aaagttgatc cattttgtcc tttataaatg cctgaaataa tcgaactatt tttgattcta
      181 aaatatcttc ataatgaact ttctcaataa ctttcaattg attccccacc ctaactaaaa
      241 aaatcgaacc tacatcattt aataaattta taactatagg tgtaaacatt attaaaataa
      301 aacccctcta ctactatatg taacgaaggg acatgatttc aaaataaaat acctttttta
      361 taaatttatt ataatatttt tcattatact atacaaccga atctaatcta acgatcctga
      421 aaagaaaaat aacggcggtt ccgcttcata ttcatttttg tccaagtgtt ctttttcaat
      481 tatttgattg acggttttcc aattttttac ttcatgctct tttaaaaaat ggctatgaaa
      541 aattctatcc ccatagattt cttcatagcc aatgctaatt tgtttcatgt catttcttta
      601 aaattcctaa aaatatataa actaaaagca taagtaacac agcaaaaatt gccgaatata
      661 taattattga aaaagcattg ctttgcattg
//
LOCUS       DAKTLH010000323          689 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.332, whole genome shotgun sequence.
ACCESSION   DAKTLH010000323 DAKTLH010000000
VERSION     DAKTLH010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.332"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(13..654)
                     /locus_tag="O0394_002902"
     CDS             complement(13..654)
                     /locus_tag="O0394_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCY0500888.1"
                     /translation="METLLCESKVINKNPKYRIIKYDSEYLMIDLASNWIVFFFPFIN
                     WLIPKTYVKITKNDYEKLNIVKPVKNKSIGWTIFAGIVLLGGTVRRNTYLFDFQLEEL
                     IVWSSCFIGFLEIIFFYCYLNKKLTLNIYNESKNNELKLRLLPSFKNICFTIFYYLFT
                     GFMSYGAFYLLVFENVQNLILYVSWLFMTMLFMFMNMHSIIDKKVHIFLKSNK"
BASE COUNT          266 a           99 c           74 g          250 t
ORIGIN      
        1 ctaaatttgt aactatttat tagactttaa gaatatatgt acttttttat ctataattga
       61 atgcatattc ataaacataa atagcatagt catgaaaagc caagaaacat ataagattaa
      121 attttgcaca ttttcaaata ccaacaagta aaatgcccca taagacatga aaccagtaaa
      181 taggtaataa aaaattgtga aacaaatatt tttaaaggag ggtaataatc ttaatttaag
      241 ttcattattt ttactttcat tataaatatt taatgttaat ttcttattta gataacaata
      301 aaaaaatata atctctaaaa acccaatgaa acagctgctc caaacaatta gttcttctaa
      361 ttgaaaatca aataaataag tatttcttct tacagtacca ccaagtaaca caatacccgc
      421 gaatatggtc catcctatcg atttattttt aactggtttg acaatattta atttttcata
      481 atcattctta gtgattttga catatgtttt cggtatgagc cagttaataa atggaaagaa
      541 gaagacaatc caattacttg ccaaatcaat cattaaatat tcactatcgt atttgattat
      601 tcgatattta gggtttttat taataacttt agattcgcaa agcaatgtct ccacatccct
      661 ttaattttat gtgtaataca tttttcgat
//
LOCUS       DAKTLH010000324          681 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.333, whole genome shotgun sequence.
ACCESSION   DAKTLH010000324 DAKTLH010000000
VERSION     DAKTLH010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.333"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            50..337
                     /locus_tag="O0394_002903"
     CDS             50..337
                     /locus_tag="O0394_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500889.1"
                     /translation="MEKGIFNYDNANVLKLDTNQLNENIKVIDDIFKNYEQIEPTIEV
                     ENGNTKLKLNGYFIASIISPLNLNKLNNLYVEEEFYHTYNELIVKYTEVKE"
     gene            343..>681
                     /locus_tag="O0394_002904"
     CDS             343..>681
                     /locus_tag="O0394_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HCY0500890.1"
                     /translation="MKKIFMMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYK
                     GNYDEIIKALKKQGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERLKNTIEI
                     EESKGISRYFD"
BASE COUNT          295 a           55 c          110 g          221 t
ORIGIN      
        1 gaagtataaa attattttgt aaaacaattt tgttattaag aggtgcgcta tggaaaaggg
       61 gattttcaat tacgataatg cgaatgtatt aaaattagat actaatcagt taaatgaaaa
      121 cataaaagtc attgacgata tatttaaaaa ttatgagcag atagagccaa ctatagaagt
      181 cgaaaatgga aatacaaaat taaaattaaa tggatatttt attgcgtcaa ttataagccc
      241 attaaattta aataaactta ataatttata tgttgaagaa gaattttatc atacatataa
      301 cgaattaatt gttaaatata ctgaggtaaa ggaataatta taatgaaaaa aatatttatg
      361 atggtacatg agttagatgt gaataaaggt ggtatgacct cttcgatgtt caatagaagt
      421 aaagagtttt atgatgcgga catacctgct gatattgtta ctttcgatta caaaggaaac
      481 tatgatgaaa ttattaaagc tttgaaaaaa caaggtaaaa tggatcgaag aacgaaaatg
      541 tataatgtat ttgagtattt taaacaaatt tcaaataata aacattttaa gtctaataaa
      601 ttgttatata aacatatttc agaaagacta aaaaatacga ttgaaattga agagagtaaa
      661 ggtatttcaa gatattttga t
//
LOCUS       DAKTLH010000325          673 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.334, whole genome shotgun sequence.
ACCESSION   DAKTLH010000325 DAKTLH010000000
VERSION     DAKTLH010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.334"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..601)
                     /locus_tag="O0394_002905"
     CDS             complement(<1..601)
                     /locus_tag="O0394_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCY0500891.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLLN"
BASE COUNT          270 a          108 c           90 g          205 t
ORIGIN      
        1 tattcaacaa attaataaat agattaaatc ccatttctct tggtgaatag tcacctcttt
       61 ttaaacgatt aaaatcatac aaagtataaa gtagaaataa caacagacta acgacggtaa
      121 taatagtgta aaaaatagga ttttgaagaa aaataccaat tagactcgca ataattaatg
      181 ttattaatgt aacgaataaa tatttgccta tactcgatgc atctccaacg aagaaataac
      241 caataatacc aaatgctata aatgcaaaaa ttgctaatgc gatattttta tagaaaatat
      301 ctggtcctaa attttgtaaa tacgtggtaa acgttgcgta tgacaataag ccaatcacta
      361 cagcgtaaat atgtgaaata attaaaccga accgtctcgc tctttcaaaa acaagtgttg
      421 ctaaaataat aatcagtaat ccaaatgaca agggttgtcg ccaacttaat ggtaaaaact
      481 gacctagata gcatcctatg ccaaatataa tccaataata cataaaataa agccacactt
      541 tacttattga ttgagcatgc tggttttgat gataatatga atgatttgta ttttgcgaca
      601 aaataattcc tccttctacc gtcgttttat gcgtcaaatt ttacagtaaa ttttgcttca
      661 tcaaaagaaa taa
//
LOCUS       DAKTLH010000326          623 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.335, whole genome shotgun sequence.
ACCESSION   DAKTLH010000326 DAKTLH010000000
VERSION     DAKTLH010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.335"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            251..436
                     /locus_tag="O0394_002906"
     CDS             251..436
                     /locus_tag="O0394_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500892.1"
                     /translation="MSEYKKKIIELIESNLTGYEISKKTGVSQYVLSQLRQGKREVDN
                     LTLNTTEKLYEYANKVL"
BASE COUNT          286 a           57 c           95 g          185 t
ORIGIN      
        1 aaacattgaa gttttgtaaa aggcgtaagg tatacaagac aaatcgttta tctttgacaa
       61 agtaattgat gatgaaaagt cgttaatatg tagaaacaaa aattgaaaat ataaaattta
      121 actattatga agtgatgata gccattaatg aatagaaaaa taataaaatc atttatagat
      181 aaaaaatacg ttaagttcac agaatttcga aaaaaagcag aaaacattgt acagatatgg
      241 agatgaaaaa atgtcagaat acaagaaaaa aataattgaa ttaattgaaa gtaatttaac
      301 aggatatgaa atttctaaaa aaactggagt ttctcaatac gtactttcac aattaagaca
      361 gggcaaacgc gaagtagata atctaaccct gaatacaaca gaaaaattat atgaatatgc
      421 caataaagtt ttgtaattta actaatgtat aaattaatca agctatgttt atttgattta
      481 actattaata aaaatcatat ggtgaatgga tattataata attaaaatac aaaaatagta
      541 gattccaatt tgtgaaaaag gagtatgtcg tttcatttag aatttattgc acgaaattag
      601 catcgaataa gggaaaataa gtg
//
LOCUS       DAKTLH010000327          616 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.336, whole genome shotgun sequence.
ACCESSION   DAKTLH010000327 DAKTLH010000000
VERSION     DAKTLH010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.336"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            410..>616
                     /locus_tag="O0394_002907"
     CDS             410..>616
                     /locus_tag="O0394_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCY0500893.1"
                     /translation="MNKKYLMIVIIILILILGGIFLKMKYDEKKYYDEQKERITIYMK
                     YNVKGYKNISFANFKENPMDGYSIS"
BASE COUNT          267 a           58 c           90 g          201 t
ORIGIN      
        1 aaactagtaa ataagtttaa ataagatttc gaaaaaatgt gtaatattat aatatgaaaa
       61 tctttaagat tatagaagaa tcatactgac taaaaagatt ttgaaagtga ttaagtattt
      121 aaaacacgcc aatcttaaac cgctattgac aaatatgaag cggaaccacc gttgtttttc
      181 ttttcaggtc caattgaata aatgttatga atagaatgga taaaaatata attatttaag
      241 tgttaaagta aaccaaatca tgatttaaga ttataagaaa gaaaaattga ctaatgttaa
      301 aaccaacaat catatgggtt gtaacgttta tacaatcatc gtaaaactca atatgcttaa
      361 tttattcaaa atgttcgtca ataagtacaa tgaaaaggag attgattttt tgaataaaaa
      421 atatcttatg attgtaatta taattttaat attgattcta ggcggaatct ttttgaagat
      481 gaaatatgac gaaaagaaat attatgatga acaaaaagaa agaataacga tttatatgaa
      541 gtacaatgtg aaaggttata aaaatataag cttcgctaat tttaaagaaa acccaatgga
      601 tggttattct attagt
//
LOCUS       DAKTLH010000328          605 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.337, whole genome shotgun sequence.
ACCESSION   DAKTLH010000328 DAKTLH010000000
VERSION     DAKTLH010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.337"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            66..263
                     /locus_tag="O0394_002908"
     CDS             66..263
                     /locus_tag="O0394_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500894.1"
                     /translation="MTELNNIINSLQSLFESESGYKISKNSGVPYQTVQDLRNGKTKL
                     EDARFRTIIKLYSYYVSLKEH"
BASE COUNT          238 a           93 c           98 g          176 t
ORIGIN      
        1 catccataca ctgactgttt taataacaac attgtatatt gttaattcaa gaaaggggat
       61 taaaaatgac agagttgaat aatattataa actctcttca atctttgttt gagtctgaat
      121 caggctataa aatttcgaaa aattcaggag ttccatatca aacagtacaa gatttaagaa
      181 atgggaaaac caaactagaa gatgctagat ttagaacgat tattaaactt tatagttact
      241 atgtctcatt aaaagaacac taatcatggg acaataaagt attgctataa taatttaatc
      301 attaaatgaa ggaatagagc ctaagacata ataaatcaat gtcttaggct ctacaatatt
      361 atattggtag tagttgaccg aatgaaaatg cccatgtaac aagcattttt caattatagg
      421 cggggcccca acatagagaa tttcaaaaag aaattctaca gacaatgcaa gttggggtgg
      481 gccccaacac agaagctgac gaaaagtcag cttacaataa tgtgcaagtt ggggagtgac
      541 aacgaaataa attttataaa atactatttc tgtcacattc ctactcccaa ttccatttaa
      601 atata
//
LOCUS       DAKTLH010000329          599 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.338, whole genome shotgun sequence.
ACCESSION   DAKTLH010000329 DAKTLH010000000
VERSION     DAKTLH010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.338"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(511..>599)
                     /locus_tag="O0394_002909"
     CDS             complement(511..>599)
                     /locus_tag="O0394_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LSM domain protein"
                     /protein_id="HCY0500895.1"
                     /translation="DGYNSIEIDTGRITYDISENKIKTIVLQ"
BASE COUNT          193 a           57 c          100 g          249 t
ORIGIN      
        1 ttaattaaga tcagttgagt agatgtgttt atttatatat gaaactgctc atttgtagcc
       61 gttgatgata taaaattaca ttattccaaa acaaggtact acagattatt gagtatcttg
      121 ttttatttta tatatttaaa aatgttaatt tgtgctgtat ttactttaat ttgacgacat
      181 ttgagaagta ttatgatggt tgtagttatt ttatgaaagt aatgtattaa caatcgaatt
      241 actaagtcta tattggtagg aatatcgatg tttagtttaa atggaatgca ctatttaagt
      301 ttttaatatg gagatgtttg tgacttttcg atgattaaga tttttatagg tgtgcatcat
      361 ttcaaataaa ctttgtgttt aaaattgagc ttaggaaatc gataggttta gatgaggata
      421 ttgttgaagt tatgtgtctt gtatccttag ttgttataaa agcgcaaaaa atagcaccgc
      481 tttctcttta tctgtgtaga aaggatgcta ttattgtaaa acaatagttt taattttatt
      541 ttctgatata tcatatgtaa ttctacctgt atcaatttct atcgaattat aaccatcaa
//
LOCUS       DAKTLH010000330          587 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.339, whole genome shotgun sequence.
ACCESSION   DAKTLH010000330 DAKTLH010000000
VERSION     DAKTLH010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.339"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..>587)
                     /locus_tag="O0394_002910"
     rRNA            complement(<1..>587)
                     /locus_tag="O0394_002910"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          137 a          172 c          116 g          162 t
ORIGIN      
        1 taaaacgatt ataggtggta caggaatatc aacctgttat ccatcgccta cgcctgtcgg
       61 cctcagctta ggacccgact aacccagagc ggacgagcct tcctctggaa accttagtca
      121 atcggtggac gggattctca cccgtctttc gctactcaca ccggcattct cacttctaag
      181 cgctccacat gtccttacga tcatgcttca acgcccttag aacgctctcc taccattgtc
      241 caaaggacaa tccacagctt cggtaatatg tttagccccg gtacattttc ggcgcagtgt
      301 cactcgacta gtgagctatt acgcactctt taaatgatgg ctgcttctaa gccaacatcc
      361 tagttgtctg ggcaacgcca catccttttc cacttaacat atattttggg accttagctg
      421 gtggtctggg ctgtttccct ttcgaacacg gaccttatca cccatgttct gactcccaag
      481 ttaaattaat tggcattcgg agtttgtctg aattcggtaa cccgagaggg gcccctcgtc
      541 caaacagtgc tctacctcca ataatcatca cttgaggcta gccctaa
//
LOCUS       DAKTLH010000331          549 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.341, whole genome shotgun sequence.
ACCESSION   DAKTLH010000331 DAKTLH010000000
VERSION     DAKTLH010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.341"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
BASE COUNT          196 a           97 c          110 g          146 t
ORIGIN      
        1 aatggtgttt taatatttaa aattgaactt ttgatatatt actatgtctg gtacacaaat
       61 caatgtttta tgctttacaa agttatattg gcagtagttg actgcagtcc acaacataga
      121 ggcttcggaa tgtcagcttc tatttcatgc aagttggtgg agctccaaca tagtggaatt
      181 ggattcccaa tttctacaga cattgcaaat tggggaaacg ggccacaaac tcagaaactg
      241 gtggaaagtc agcttaaaat aacatgcaag ttggcgaggc cccaaaatag tgagatcgga
      301 tttctaattt ctacagacat tgcaaattag ggaaacgggc cacaaactca gaagttggtg
      361 gaaagtcagc ttaaaataac atgcaagttg gcggggccac aacatagaaa aattggatcc
      421 tcaatttcta caaacaatgt aagttgggga aacagcccca acactgaaac tagcagaaag
      481 tcagcttcta tgaatataat aaaaaagcta ggtaacaaaa tgctacctaa cttcatattc
      541 aagataatc
//
LOCUS       DAKTLH010000332          536 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.342, whole genome shotgun sequence.
ACCESSION   DAKTLH010000332 DAKTLH010000000
VERSION     DAKTLH010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.342"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            18..533
                     /locus_tag="O0394_002911"
     CDS             18..533
                     /locus_tag="O0394_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HCY0500896.1"
                     /translation="MKIKTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVIELPEG
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDQDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKID
                     QTSINLIGRKI"
BASE COUNT          199 a           70 c          103 g          164 t
ORIGIN      
        1 tacaaggaga gagcaacatg aaaataaaaa ctgaatttaa agggaacaat ataccatatg
       61 aatacgcagc aggtgcagat gtgagtgatt ctattaacgg gaatccaatt aagtcatttc
      121 catttgaagt aattgaatta ccggaaggga ctaaatatct tgcttggtct ttaattgact
      181 atgatgcaat tcctgtatgt ggctttgctt ggattcattg gagtgtagct aatgtaagtg
      241 ttagtggcaa ttcaatttct ataaaagcag atttatcaag aacaaagggc gactatgtac
      301 aaggtaaaaa tagctttact agtgggttgt tggctgaaga tttttcagaa atagaaaatc
      361 actatgtagg acctacacca cctgatcaag atcatcaata tgaattaaca gtttatgcgt
      421 tagatcattc tttaaatttg aagaatgggt tctacttgaa tgaattttta aaagaagtaa
      481 atcaacataa aattgatcaa acaagtatta accttatagg aagaaaaatt taatac
//
LOCUS       DAKTLH010000333          527 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.343, whole genome shotgun sequence.
ACCESSION   DAKTLH010000333 DAKTLH010000000
VERSION     DAKTLH010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.343"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            315..527
                     /locus_tag="O0394_002912"
                     /pseudo
     CDS             315..527
                     /locus_tag="O0394_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          211 a           55 c           92 g          169 t
ORIGIN      
        1 catttgaatc tcggaaaatg ccactattta tgacgataat ttttacaatc taaaagcagt
       61 aaagactcat aatataacca aaaacattaa taaaagtttg ttaaatgata aggggatgag
      121 tattggtgaa tttactcaaa aggtttaagg gaaaaatcct acctggagag attcaaaaac
      181 taaatggact atttccaaaa ataaagggca atcacatggt ggttcttatt ggaagttaat
      241 aaataacaaa ggaaaaagaa tagcttcttt aactaaagaa ggaaaaatct taagggaata
      301 aggtgttgta aattatgtgc tttgatataa ataatttgga tataaaaaag ttgaatttta
      361 gaaaggtaaa aaacgcaatt catttaagtt taagtggcga taaataccag tttttggagg
      421 acggtaaaac tatcaataat acttattttt agctgtatat gataatgcaa taaacatttt
      481 tgaagatttg ttttcgcctt ctgattctat aaatttggtt catgtag
//
LOCUS       DAKTLH010000334          525 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.344, whole genome shotgun sequence.
ACCESSION   DAKTLH010000334 DAKTLH010000000
VERSION     DAKTLH010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.344"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            80..400
                     /locus_tag="O0394_002913"
     CDS             80..400
                     /locus_tag="O0394_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0500897.1"
                     /translation="MQSIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
BASE COUNT          193 a           73 c           86 g          173 t
ORIGIN      
        1 ttttgaagct aaaatttttt attatgatat aaatttagct ataataaaaa ttattaaact
       61 atctagagga gttgttacaa tgcaatcaat caaaagtaat gaatcattta aatctgtaat
      121 taatagcgat acacctgtaa ttgttaaatt tgaggcagga tggtgcccag actgtcgtgc
      181 tatggattta tggattgacc caatcgtaga acaatataat gattaccaat ggtatactgt
      241 taatcgtgat gaattagaag atgtagttgt tgaaaatgaa gttatgggta tccctagctt
      301 gctagtattt aaaaacggcg ataaaattgc acaccttcat tcagcaaatg ctaagtcacc
      361 tgaacaagtt gaatcatttt tagcagaaac ttttaaataa atactaaaat aaaaatgtgt
      421 atatactgca caataaaaag ctgagactgt tattgtaccg actctagtaa gttagattaa
      481 atctctaact cgatagatgt ccatcaattc atgactcagc tttta
//
LOCUS       DAKTLH010000335          514 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.345, whole genome shotgun sequence.
ACCESSION   DAKTLH010000335 DAKTLH010000000
VERSION     DAKTLH010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.345"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            127..333
                     /locus_tag="O0394_002914"
     CDS             127..333
                     /locus_tag="O0394_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500898.1"
                     /translation="MNEIETIISEIEKLLTNNTPYSISKNSGVPRQTVTDLKVGKTKI
                     KEAKFKTIIKLYEYQRTLENKTEC"
BASE COUNT          217 a           55 c           75 g          167 t
ORIGIN      
        1 caatactatt aggtattagt ttatgaatta actagtttgg caactagaaa aaaacaatgt
       61 attgctaaac gtagttactg cattaggatt attataatta taaatagaaa gagagtgtga
      121 ttaataatga atgagattga aactattata agtgaaatag aaaagttatt aactaacaat
      181 acaccatata gtatttcaaa aaactcaggt gtaccacgtc aaacagttac tgatttaaag
      241 gtaggtaaaa ctaaaataaa agaagctaaa tttaaaacga taatcaagtt atatgaatat
      301 caaagaacat tagaaaataa aacagaatgt taacaaaaat attttgttag gttaggtgga
      361 caagaaatta aatttctaat gatttcatgg ttgtctgtcc acgttttgtc cacctcaata
      421 agataatctc ttagttttag aaattgtaca tggttaaata atcaaaaatg catattatga
      481 aattatcatt gccattaaat cttgaatgat cttt
//
LOCUS       DAKTLH010000336          512 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.346, whole genome shotgun sequence.
ACCESSION   DAKTLH010000336 DAKTLH010000000
VERSION     DAKTLH010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.346"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
BASE COUNT          176 a           70 c           73 g          193 t
ORIGIN      
        1 gaatttgaaa tattagtaaa tgcgttataa aaaaatagag catcctccac gttatggagg
       61 tgctctgttt tttattgaaa agtatcaagt taattaattt aatatgctta ataagttcta
      121 ccttgacctt tttctctagc ttctgttcga tctcttatgt actcagtaca tactggattt
      181 tctgttaatt catcaactgt attagtttaa tcagaagacg tgtctacttt gtaagcttct
      241 aagaacttat tatatgatat agcgtttgag ttttgttgtt cttctataaa tttctgtagt
      301 tatttttcaa aaaccgcatc attaactgat aagcagaagc gtatcacaaa taaaactaaa
      361 aaatagattg tgtataatat aaaaggaagg gatttatatt aaaattttga attcaaaaat
      421 tattgaaagg gaagctacct tagaaattga atctatggcc actaactgct tttttatatc
      481 accacttatt aaaaaatgta agaatattcc tc
//
LOCUS       DAKTLH010000337          506 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.347, whole genome shotgun sequence.
ACCESSION   DAKTLH010000337 DAKTLH010000000
VERSION     DAKTLH010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.347"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            271..506
                     /locus_tag="O0394_002915"
                     /pseudo
     CDS             271..506
                     /locus_tag="O0394_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          204 a           74 c           68 g          160 t
ORIGIN      
        1 tgtaatttta ctttgaaata ctttaaaaaa ataagacact ttgcccaact tgcacataaa
       61 tgtaaaattc aataaaataa attttctgtg ttggatccct tcgtataatt taataaatac
      121 tactaaacta aattaacgag gtgccttatg tataaaaatt ataacatgac ccaacttaca
      181 ctaccaatag aaacttctgt tagaattcct caaaatgata atacgcgata tgttaatgaa
      241 attgttgaaa cgatatctga tagcgaattc gatgaattta tatgtcctaa taacaaaaga
      301 ataggtttta aaagatatgc ataccgtaat gatagatata gttttaaacg tgacttcaag
      361 ctatatgaat gtgatgactg ttcatcatgt tctttgagac atcaatgcat gaagccaaat
      421 tcgaaatcca ataagaaaat tatgaagaat tataattggg aatactttaa agcccaaatt
      481 aatcaaaagc tttctgaacc agaaac
//
LOCUS       DAKTLH010000338          473 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.348, whole genome shotgun sequence.
ACCESSION   DAKTLH010000338 DAKTLH010000000
VERSION     DAKTLH010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.348"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..>473
                     /locus_tag="O0394_002916"
     CDS             <1..>473
                     /locus_tag="O0394_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0500899.1"
                     /translation="LDIINEKQNEWRQRLMVTPFTFTSYYISKVVKTMLQFAIAILVI
                     FMVGHFYKGVAMSAVQWLESGIFLWLGASLLITFGILFSLLNDIQKTSALANIVTIGL
                     AVLGGLWFPINTFPNWLQHVAHVLPSYHLRKLGVDIASNHHINLISFAIILLYAL"
BASE COUNT          150 a           61 c           80 g          182 t
ORIGIN      
        1 cattagatat tattaatgaa aaacaaaatg aatggcgcca aagattaatg gtaacaccat
       61 ttacttttac tagttattat atttcaaaag tagtgaaaac tatgctgcaa tttgcaatag
      121 cgatattagt tatttttatg gttggacatt tttataaagg tgttgcaatg agtgcagttc
      181 aatggttaga gtcaggaata tttttatggt taggtgcgtc tctattaata acttttggca
      241 tattattttc tttgttaaat gatattcaaa aaacaagtgc tttagctaat atcgtaacaa
      301 ttggtttagc agtattaggt ggattgtggt ttccgataaa cacatttcca aattggcttc
      361 aacatgttgc tcatgtttta ccgagctatc atttgcgtaa actaggtgta gatattgctt
      421 caaatcatca tatcaattta atatcatttg ctataatact cttgtatgct tta
//
LOCUS       DAKTLH010000339          464 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.350, whole genome shotgun sequence.
ACCESSION   DAKTLH010000339 DAKTLH010000000
VERSION     DAKTLH010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.350"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            293..397
                     /locus_tag="O0394_002917"
     CDS             293..397
                     /locus_tag="O0394_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500900.1"
                     /translation="MFKVNYSILSYYPEYNIAVSWQRLREGKTIKNKI"
BASE COUNT          163 a           64 c           80 g          157 t
ORIGIN      
        1 gatcgtcttg aaatgctccc ttcaaagttt tcattttttc aatgtcgact tcgaaggggg
       61 cattttcatt aaattgttat agctttttat atttgtataa tgaacatata agtttaagaa
      121 ggtgcgagtg aaggaaataa aaaagctcaa atgtaccaaa ttgttaatct taataaatct
      181 ctactttata aagattgaat ggacattcga gcgttaatca gtcaggaggg actttccctc
      241 ctacaattta ataataatac ttgcttcacc actatacaag gagtgagttg ttatgttcaa
      301 agtgaattat tcgattttaa gttattatcc agaatataat atcgcagtaa gttggcaacg
      361 tttaagagaa ggaaaaacaa taaaaaacaa gatttaatac tgctgcgtca tgaggcgctt
      421 gaacattatt tgatgaataa gtataatttc aactatgatt atgc
//
LOCUS       DAKTLH010000340          452 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.351, whole genome shotgun sequence.
ACCESSION   DAKTLH010000340 DAKTLH010000000
VERSION     DAKTLH010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.351"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(269..448)
                     /locus_tag="O0394_002918"
     CDS             complement(269..448)
                     /locus_tag="O0394_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500901.1"
                     /translation="MKFKTTKECKSNNIFKRSQEINNRESEKGCLWGISMLILLFLLI
                     LFGITACSSSIHFIN"
BASE COUNT          181 a           64 c           57 g          150 t
ORIGIN      
        1 agtaacacac agaagtgtag aaaataaaaa atattggtaa ataaaatcaa taagtttaac
       61 caatatgttg ctcgcttcat accgtatatt gcaacaaaaa ttccgattaa gaaaaatata
      121 gcccctatga taaaacagaa atccgatgct gaattattaa aaaatgaggt gtttagagtt
      181 agaaaatgag ttaatgagtt gactataact aataagatat taattatatt tgtatggttc
      241 ttcacatgat acctccaagt aaaaaaatct aattaataaa gtgaatgctt gatgaacaag
      301 cagttattcc aaacagaatc aataagaaaa gtagaatcaa catgctaatg ccccataaac
      361 aacccttttc actttctcta ttattaattt cttgacttct tttaaagata ttattacttt
      421 tacattcttt agttgtttta aatttcacgt tt
//
LOCUS       DAKTLH010000341          450 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.352, whole genome shotgun sequence.
ACCESSION   DAKTLH010000341 DAKTLH010000000
VERSION     DAKTLH010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.352"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..179
                     /locus_tag="O0394_002919"
     CDS             <1..179
                     /locus_tag="O0394_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCY0500902.1"
                     /translation="SSDLSKGFPRIPILKDVYGFVEIGRELVELHLNYEKQLNWDGVE
                     IIYNNMNPNYKVEK"
     gene            176..>450
                     /locus_tag="O0394_002920"
     CDS             176..>450
                     /locus_tag="O0394_002920"
                     /inference="COORDINATES: protein motif:HMM:NF037202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500903.1"
                     /translation="MKHPKKGVLDTVIYNTDITVTNIPNKVYNYIVNGKPAIEWIIDQ
                     YNVSIDKKSGILDDPNEFSEDPNYILNLLLSVITVSMKTLGLIDKLP"
BASE COUNT          174 a           50 c           83 g          143 t
ORIGIN      
        1 attcaagtga tttgagtaaa ggttttccgc gaataccgat attaaaggat gtatatggat
       61 ttgttgaaat tggtagggag ttagtggaat tacatttaaa ttatgaaaaa caactcaatt
      121 gggatggtgt agaaataatt tataataata tgaatccgaa ttataaagtt gaaaaatgaa
      181 gcaccctaaa aagggcgtgt tggatacagt aatatacaat accgatatta cagttactaa
      241 cattccaaat aaagtatata attatattgt gaatggcaag cctgctattg aatggattat
      301 agatcagtac aacgtaagca tcgacaagaa atctggaata ttagatgatc ctaatgaatt
      361 tagtgaagat ccgaattaca ttttaaatct gcttttatca gttattactg taagtatgaa
      421 aactcttgga ttaatagata aattaccaga
//
LOCUS       DAKTLH010000342          449 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.353, whole genome shotgun sequence.
ACCESSION   DAKTLH010000342 DAKTLH010000000
VERSION     DAKTLH010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.353"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..392
                     /locus_tag="O0394_002921"
     CDS             <1..392
                     /locus_tag="O0394_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCY0500904.1"
                     /translation="NKKQQIVDYMARHFGVYYSLTTISRDLQELEIYKIPVENKKYIY
                     KKINQTNQLSAKKQLEIFSDEIIEFITLNNYVLIKTSPGFAQSISYYIDQLQMKEILG
                     IIGGNDTLMILTSSNEIAEFVCYQLFP"
BASE COUNT          198 a           49 c           58 g          144 t
ORIGIN      
        1 ttaataaaaa acaacaaata gtggattaca tggcaagaca ttttggagtt tactatagct
       61 tgacaactat ttctcgtgac ttacaagaat tagaaattta caaaatccct gttgaaaata
      121 aaaagtatat ttacaagaaa ataaatcaaa caaatcaatt aagtgcaaaa aaacaattag
      181 aaatatttag tgatgagatt attgaattta taacgctaaa taactatgtc ttaataaaaa
      241 catctcctgg ctttgctcaa agtataagtt attacataga tcaattacaa atgaaagaaa
      301 tattaggaat tattggaggt aacgatactt tgatgatttt gacttcttca aatgaaatag
      361 cagaatttgt ttgttatcaa ttattccctt aaaaattaaa tgattatatt aataggaatg
      421 attactaata caaaaaaatg agtaaaaaa
//
LOCUS       DAKTLH010000343          447 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.354, whole genome shotgun sequence.
ACCESSION   DAKTLH010000343 DAKTLH010000000
VERSION     DAKTLH010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.354"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
BASE COUNT          121 a           94 c           59 g          173 t
ORIGIN      
        1 ttcaataaag tatataaaag agcagtaaga tcattttcaa ttagataccg tcttactgct
       61 cttctctatt tatacaatat tttgttttcg atgatatcat tttaaattta tataaacaca
      121 aaattaaagt tgtacatact tagacaatcg aaagtgtaca ttattaaatt atcatttcca
      181 gttctactct agcggaacgt aagttggcta ccatcctcgc taagaacctt tcttgacttg
      241 tgacaatcgc ttgcttcttt cctctccttc ggctctcact tactcattta gctctactaa
      301 actcgttgcg ttcttttctc gtttcgtcag attcaaacgt tttcacttcg ccaagccatt
      361 tttctttgtg tttgcttttt attttgacgt tttagacata aaaaaaagag accttgcggt
      421 ctcaatgcgg ctcatcgcat ccatttt
//
LOCUS       DAKTLH010000344          442 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.355, whole genome shotgun sequence.
ACCESSION   DAKTLH010000344 DAKTLH010000000
VERSION     DAKTLH010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.355"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..>442)
                     /locus_tag="O0394_002922"
     CDS             complement(<1..>442)
                     /locus_tag="O0394_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
                     /protein_id="HCY0500905.1"
                     /translation="LFSESGHHFDAILFLKEDTTLQNNVYIIPFGQAYHDINNLIDYD
                     FGIDFAERAIKNEDIVNKNVNFFQQNRLKEIVNYRRNSVDYVRPSESYISVQGHPQNP
                     QIFGKTMTCGTSISLRVPNRKQQFIDKISVIIKEINAIINLPQKI"
BASE COUNT          144 a           64 c           58 g          176 t
ORIGIN      
        1 aattttttga ggaagattaa taatagcgtt tatttctttg attatcacac taattttatc
       61 tatgaattgc tgctttctat tcggtacacg caatgaaata cttgtaccac aagtcattgt
      121 ttttccaaaa atttgaggat tctgtggatg tccttggact gatatataag attctgaagg
      181 tctaacgtaa tctacactat tccttctata attaacaatc tctttaagcc tgttttgttg
      241 aaaaaaatta acatttttat taactatgtc ttcatttttg attgctcttt ctgcaaaatc
      301 aattccgaag tcataatcaa tcaaattatt tatatcatga tatgcttgtc caaaaggtat
      361 aatatataca ttattttgta atgttgtatc ttctttgaga aataatattg catcaaaatg
      421 atgaccggat tctgaaaata aa
//
LOCUS       DAKTLH010000345          442 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.356, whole genome shotgun sequence.
ACCESSION   DAKTLH010000345 DAKTLH010000000
VERSION     DAKTLH010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.356"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
BASE COUNT          139 a           73 c           66 g          164 t
ORIGIN      
        1 acttattaca agtacatcat atattagttg ataacgatta tcaatgtcgc gtggatttgt
       61 gacacatttc ttttaaaaat tcacaaggtt atggggcaga aatgataaag agccactaat
      121 gatttattat gtagtggttc tgggagtggg acagaaatga tattttcaca aaatttattt
      181 cgtcgtccca ccccaacttg cattgtctct agaaattggg aatccaattt ctctttgttg
      241 ggtccctgaa tatagccttg tagagtctag tacattgatt tgtatcccaa tgtccctata
      301 attgattatt cgctttatct aatgatccta tgactcaact attaaatcat ttttcgaaat
      361 acttaattct aatataatta aattcattta ttgtaatatt gcaaaaatac attgcacacc
      421 ttgttcatca atgctataat ta
//
LOCUS       DAKTLH010000346          407 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.358, whole genome shotgun sequence.
ACCESSION   DAKTLH010000346 DAKTLH010000000
VERSION     DAKTLH010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.358"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..>407
                     /locus_tag="O0394_002923"
     CDS             <1..>407
                     /locus_tag="O0394_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HCY0500906.1"
                     /translation="KTRPELQSILKSFGIKTTGNKPDLIKRIDDNFHIINNLDLPYVY
                     IPTKKGEEILKKTEYLTSFIQSYGEISLERAYYLVENYIDENCDDKVAEIYKFEFQRK
                     YDNGEFDFNHGYNFELNMLIDHYKRDVKDYDNA"
BASE COUNT          170 a           44 c           64 g          129 t
ORIGIN      
        1 aaagacaaga cctgaattac aaagtatttt aaaaagtttt ggtataaaaa caacaggtaa
       61 taaacctgac ttaattaaac gtattgacga caattttcat attattaata acttagattt
      121 accatatgta tatataccaa ctaagaaagg agaagaaatt ttaaagaaaa cagagtactt
      181 aacctctttt atacaaagtt atggtgaaat ttctcttgag cgtgcttatt atttggttga
      241 aaactatata gatgaaaatt gcgatgataa agttgcagaa atatacaagt ttgaatttca
      301 aagaaaatat gacaatggcg agtttgattt taatcatgga tataatttcg aattgaatat
      361 gttgatagat cactataaaa gagatgtaaa agactacgat aatgcca
//
LOCUS       DAKTLH010000347          396 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.359, whole genome shotgun sequence.
ACCESSION   DAKTLH010000347 DAKTLH010000000
VERSION     DAKTLH010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.359"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..156
                     /locus_tag="O0394_002924"
     CDS             <1..156
                     /locus_tag="O0394_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500907.1"
                     /translation="VVTPLFGIGSIVCSINYKKYDAEKGSKLLQIAIIVTIIAFVLNL
                     LAYLGLR"
     gene            288..>396
                     /locus_tag="O0394_002925"
     CDS             288..>396
                     /locus_tag="O0394_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HCY0500908.1"
                     /translation="MTFEEKLSEMYSEIANKISSMIPVEWEKVYAMAYID"
BASE COUNT          147 a           35 c           84 g          130 t
ORIGIN      
        1 gtagttacac cgctatttgg tattggaagt attgtttgta gtattaatta taaaaaatac
       61 gatgctgaaa aaggttcgaa gttattgcaa attgcaatta tcgtaacaat aattgctttt
      121 gttttaaatt tattagcata tttaggttta agataagtaa aacgaatttg aagaagcatt
      181 aagcgacatt tgggtgttgt ttaatgcttt ttatttaacg gaaataagtg tagagaataa
      241 attaatagtt taatcaagag atatttcgaa cacatagggg cgataacatg actttcgaag
      301 aaaaattaag tgaaatgtat agcgagattg cgaataagat tagcagcatg ataccggtag
      361 agtgggaaaa ggtatatgca atggcatata tagatg
//
LOCUS       DAKTLH010000348          368 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.361, whole genome shotgun sequence.
ACCESSION   DAKTLH010000348 DAKTLH010000000
VERSION     DAKTLH010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.361"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            94..368
                     /locus_tag="O0394_002926"
                     /pseudo
     CDS             94..368
                     /locus_tag="O0394_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187510.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
BASE COUNT          147 a           48 c           47 g          126 t
ORIGIN      
        1 acttaaaaat ataaattcga tttaataatt aatttaaatt tagttaatca attttgcatc
       61 tattttgttg taagctatat aaaaggagtg ataatgatgg tgaaaaaaac aaaatccaat
      121 tcactaaaaa aagttgcaac acttgcatta gcaaatttat tattagttgg tgcacttact
      181 gacaatagtg ccaaagccga atctaagaaa gatgatactg atttgaagtt agttagtcat
      241 aacgtttata tgttatcgac cgttttgtat ccaaactgga gacttttaac ataaaattac
      301 ttatcattca aaaagtaaaa cagcataata tcaaggttta taactttatc attatcaata
      361 atacctca
//
LOCUS       DAKTLH010000349          350 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.362, whole genome shotgun sequence.
ACCESSION   DAKTLH010000349 DAKTLH010000000
VERSION     DAKTLH010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.362"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..>350)
                     /locus_tag="O0394_002927"
     CDS             complement(<1..>350)
                     /locus_tag="O0394_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500909.1"
                     /translation="EIINWLFSNYKKLDEETAEKYMIFLTWIFSATNNKLRDLGTKSL
                     VKLFKTFPTKIIGLLKLFENNNDPYIVERLYASVLGATLRIDICEIHIEIANYIYEEI
                     FDKEMVYPHILMRD"
BASE COUNT          127 a           51 c           34 g          138 t
ORIGIN      
        1 atctctcatt aaaatatgag gatataccat ttctttatca aatatttctt cataaatata
       61 attagcaatc tctatatgta tttcacaaat gtcgattcga agtgtcgccc ctaaaacaga
      121 agcatataat ctttcgacta tatatggatc attgttattt tcaaataatt ttaataaacc
      181 aataatttta gttggaaatg ttttgaataa ttttactaaa gatttagtac cgagatctct
      241 taatttatta ttagttgcag aaaatatcca cgttaaaaaa atcatatatt tttccgcagt
      301 ttcttcatct aatttcttat agttactaaa tagccaatta ataatttcaa
//
LOCUS       DAKTLH010000350          327 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.364, whole genome shotgun sequence.
ACCESSION   DAKTLH010000350 DAKTLH010000000
VERSION     DAKTLH010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.364"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..190
                     /gene="agrB"
                     /locus_tag="O0394_002928"
     CDS             <1..190
                     /gene="agrB"
                     /locus_tag="O0394_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HCY0500910.1"
                     /translation="TKKKPIPVRLIKRKKYYAIIVSLTLFIITLIIKEPFAQFIQLGI
                     IIEAITLLPIFFIKEDLK"
     gene            187..327
                     /locus_tag="O0394_002929"
     CDS             187..327
                     /locus_tag="O0394_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HCY0500911.1"
                     /translation="MNTLFNLFFDFITGILKNIGNIAAYSTCDFIMDEVEVPKELTQL
                     HE"
BASE COUNT          121 a           51 c           41 g          114 t
ORIGIN      
        1 aactaaaaag aagcccattc ctgtgcgact tattaaacga aaaaaatatt atgcgattat
       61 tgttagttta acccttttca ttatcacact tatcatcaaa gagccatttg cccaattcat
      121 tcaattaggc atcataatag aagctattac attattacct attttcttta ttaaggagga
      181 cttaaaatga atacattatt taacttattt tttgatttta ttactgggat tttaaaaaac
      241 attggtaaca tcgcagctta tagtacttgt gacttcataa tggatgaagt tgaagtacca
      301 aaagaattaa cacaattaca cgaataa
//
LOCUS       DAKTLH010000351          319 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.365, whole genome shotgun sequence.
ACCESSION   DAKTLH010000351 DAKTLH010000000
VERSION     DAKTLH010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.365"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            124..319
                     /locus_tag="O0394_002930"
                     /pseudo
     CDS             124..319
                     /locus_tag="O0394_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          125 a           59 c           38 g           97 t
ORIGIN      
        1 taaaaaataa gacactttgc ccaacttgca cataaatgta aaattcaata aaatgaattt
       61 tctgtgttgg gtcccttctt ataatttaat aaataccact aaactaaatt aacgaggtgc
      121 cttatgtata aaaattataa catgccccaa ctacactacc aatagaaact tctgttagaa
      181 tccctcaaaa tgatatttca cgatatgtta atgaaattgt tgaaacaata cctgatagcg
      241 aattcgatga attcagacat catcgtggcg caacatccta tcatccaaaa atgatgttaa
      301 aaatcatctt atatgcata
//
LOCUS       DAKTLH010000352          311 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.366, whole genome shotgun sequence.
ACCESSION   DAKTLH010000352 DAKTLH010000000
VERSION     DAKTLH010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.366"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..>311
                     /locus_tag="O0394_002931"
     rRNA            <1..>311
                     /locus_tag="O0394_002931"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           88 a           58 c           94 g           71 t
ORIGIN      
        1 ctgggaggac catctcctaa ggctaaatac tctctagtga ccgatagtga accagtaccg
       61 tgagggaaag gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgct
      121 tacaagtagt cagagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga
      181 gttacgattt gatgcaaggt taagcagtaa atgtggagcc gtagcgaaag cgagtctgaa
      241 tagggcgttt agtatttggt cgtagacccg aaaccaggtg atctaccctt ggtcaggttg
      301 aagttcaggt a
//
LOCUS       DAKTLH010000353          310 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.367, whole genome shotgun sequence.
ACCESSION   DAKTLH010000353 DAKTLH010000000
VERSION     DAKTLH010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.367"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            73..>310
                     /gene="agrB"
                     /locus_tag="O0394_002932"
     CDS             73..>310
                     /gene="agrB"
                     /locus_tag="O0394_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HCY0500912.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTIAYILNIFLFTLITNLTFYLIRRHAHGAHAP"
BASE COUNT          113 a           37 c           51 g          109 t
ORIGIN      
        1 ctacagttag gcaatataat gataaaagat tgtactaaat cgtataatga cagtgaggag
       61 agtggtgtaa aattgaatta ttttgataat aaaattgacc agtttgccac gtatcttcaa
      121 aagagaaata acttagatca tattcaattt ttgcaagtac gattagggat gcaggtctta
      181 gctaaaaata taggtaaatt aattgttatg tatactattg cctatatttt aaacattttt
      241 ctgtttacgt taattacgaa tttaacattt tatttaataa gaagacatgc acatggtgca
      301 catgcacctt
//
LOCUS       DAKTLH010000354          301 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.369, whole genome shotgun sequence.
ACCESSION   DAKTLH010000354 DAKTLH010000000
VERSION     DAKTLH010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.369"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
BASE COUNT          117 a           53 c           21 g          110 t
ORIGIN      
        1 gaatatgact tcatattaaa tcaaacctca cattcttatt agattgcaaa ttcaatagtc
       61 ctaccagaat tgaaatcaaa acattcaaac tattttatcg ttttaacttc ctcgaccatt
      121 ttcacctcct tataatctat ataaattatg tttaccacac gattctttac actcgaaaaa
      181 ttaattttgt taagaaacat ttagccatgt aactttgtaa taaaaacatt ttcaaataaa
      241 tgtaccaaaa actttcatga aataaagtat acttttcaaa tctattaatt ttagaatcat
      301 a
//
LOCUS       DAKTLH010000355          287 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.denovo.370, whole genome shotgun sequence.
ACCESSION   DAKTLH010000355 DAKTLH010000000
VERSION     DAKTLH010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.denovo.370"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(<1..>287)
                     /locus_tag="O0394_002933"
     rRNA            complement(<1..>287)
                     /locus_tag="O0394_002933"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           67 a           88 c           59 g           73 t
ORIGIN      
        1 cttccacctc gaacctatta acctcatcat ctttgaggga tcttataacc gaagttggga
       61 aatctcatct tgaggggggc ttcatgctta gatgctttca gcacttatcc cgtccacaca
      121 tagctaccca gctatgccgt tggcacgaca actggtacac cagaggtatg tccatcccgg
      181 tcctctcgta ctaaggacag ctcctctcaa atttcctacg cccacgacgg atagggaccg
      241 aactgtctca cgacgttctg aacccagctc gcgtaccgct ttaatgg
//
LOCUS       DAKTLH010000356         6870 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.guided.135, whole genome shotgun sequence.
ACCESSION   DAKTLH010000356 DAKTLH010000000
VERSION     DAKTLH010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.guided.135"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..83
                     /locus_tag="O0394_002934"
     CDS             <1..83
                     /locus_tag="O0394_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCY0500913.1"
                     /translation="FVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(255..1226)
                     /locus_tag="O0394_002935"
     CDS             complement(255..1226)
                     /locus_tag="O0394_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0500914.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            1350..1769
                     /locus_tag="O0394_002936"
     CDS             1350..1769
                     /locus_tag="O0394_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0500915.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            complement(2295..3140)
                     /gene="blaZ"
                     /locus_tag="O0394_002937"
     CDS             complement(2295..3140)
                     /gene="blaZ"
                     /locus_tag="O0394_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCY0500916.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            3247..5004
                     /gene="blaR1"
                     /locus_tag="O0394_002938"
     CDS             3247..5004
                     /gene="blaR1"
                     /locus_tag="O0394_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCY0500917.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            4994..5374
                     /gene="blaI"
                     /locus_tag="O0394_002939"
     CDS             4994..5374
                     /gene="blaI"
                     /locus_tag="O0394_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCY0500918.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            5639..6217
                     /locus_tag="O0394_002940"
     CDS             5639..6217
                     /locus_tag="O0394_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0500919.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(6440..6625)
                     /locus_tag="O0394_002941"
     CDS             complement(6440..6625)
                     /locus_tag="O0394_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500920.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
BASE COUNT         2574 a          951 c          945 g         2400 t
ORIGIN      
        1 ttttcgttca catcatagca ccagtcatca gtggctgtgc tgttgcgtat tatacttatt
       61 ggcttagtaa acgcaacaaa taattaatag gcgactttat aagccagcat cgaaaaaatc
      121 cccacactac tgcaatagtg tggggattgg tgcattaatt gttaatcatt ttcgttatat
      181 aaattataac actggttagt agagaaatgc aaaatgattg aattaatagt aaatgtatta
      241 atgaatactc acacttatac ttttaaaata gttcttccta aatgttgtcc ttttaaaagc
      301 tgatctatcg ttttatagaa ttcttcaaat ccaatttcat tgtatagaag tgaatttcct
      361 atattccatt catttgctaa cttactccat atatcttcac ggatattatg atcaacatat
      421 acagaatcaa tgccaattaa tttaatacct ctaagaataa acggtaatat attagcgtta
      481 agtgaatttc ctgctacatt tccacataca gtagcaacac cttgataatt tagacgtttt
      541 aaaccgtata atgttacatt actccccact gtatctaaga aataatcaaa aatacctttt
      601 ttaagcggtt tattatcatc ttcaattatt attacttgtt ttgcacctaa tttttttgcg
      661 atttctattt gcttttcttt tcttacgata gcagtgatat tttcatatcc aattttatgt
      721 aaaatttgta tagcaacact gcccacacca cctgtagccc cagacactaa aatttcaggt
      781 tgtgatttga tattcattcc atatttttct agttcaaaaa ttgataatgc tgccgtaaaa
      841 ccggctgtgc catataccat agactcttta atagacattt cttggggaat aggaacaatc
      901 cactctccag gcacttgtgc atattctgaa aatccacctg tatgtgtcac gccaacatca
      961 taaccagtaa ctaatacatt atcgccttct ttaaacttat cattcgaact tgattcaaca
     1021 atacctgcaa aatctatacc aggaatcata ggataatctc taattacacc gcctttatat
     1081 tgtaaagcta gcatatcctt ataatttatt gaagagtata ccacttttac taatacatca
     1141 cccttcgata aatgagattc atcaactttt tctactttat aactaacatt tgatgcactt
     1201 tttctcacta ctaccgcatt gaacatttat tagccttctt tcacaattgt tttgtagaag
     1261 tatgtttttg ttgcaaaact attttcctac aaaacttatc atgatatgat aagcgagtca
     1321 atactaatga tttaagagag gttttagttg tggaattaga taattgtata aattatttgt
     1381 tgagtacttc tcagaataga gtttttaaac attttaaaag tttattaagt ccctataata
     1441 ttactccagc agaatatggt gttttgaatt gcttgattaa tagtaatttg aatacaccta
     1501 aagcgattgg taatacttta aatttagaac cttcaacaat atctggaata ttagataaga
     1561 tgtccaaaaa ggatctaata acacgtcaaa ctgataatga aaatagaaga actgtgctaa
     1621 taaacagtac aaataaagca aaagacttat ggccaacatt agaaaagaaa gcctatcaac
     1681 ttaataatca ttacttcgct aatttaacta atgaagaaac tgtagtgttt aaaaagatat
     1741 tactaaagat aaataaaact acgttttaat ttttacgtta taaaaaagtt gaagtgcgaa
     1801 ttataatcga gtaaatataa atgatagctt agcttaatta tatattaaag ccaactatac
     1861 ttttattccc gaaaaaccta ctatccaaat ttctaagata ctttagcctc tataataaac
     1921 atattgtaat ataatttatg tattatttag gtttacccta atcgaacaat aaaaaatact
     1981 tgtaaaatgc tattgtcact ttgcttgata tatgagaatt atttaacctt ataaatgaga
     2041 aaaaagcaac gcactttaaa taagatacgt tgctttttcg attgatgaac acctataatt
     2101 aaactattca tctattattt atgatttttt gtatatacaa tatttctagt ttgttaaaga
     2161 gaattaagaa aataaatctc gaaaataata aagggaaaat cagtttttga tatcaaaatt
     2221 atacatgtca acgataatac aaaatataat acaaactata agatgttatc agtatttatt
     2281 atgcatttag aatattaaaa ttccttcatt acactcttgg cggtttcact tatcaactta
     2341 tcatttggct tatcactttt attgtcttta ttcgtaaaaa tgactaaaac aataggttca
     2401 gattggccct taggataaac aaaagcaaca tcatttctag aagcatatgt tattgcttga
     2461 ccacttttat cagcaacctt atagtctttt ggaacaccat ctttaattaa agtgtctccg
     2521 tttttattat taaacattaa atcaagtaag aaatttttat tttttttgct taattttcca
     2581 tttgcgataa gtttatttaa agtcttgccg aaagcagcag gcgttgaagt atcttttttg
     2641 ctctttggtg aatagtaatt taattctatc tcatatctaa ctggatttgt tactttatct
     2701 cctagttctt ttagacgttg tttaactttt ttgattccac cgatttcttt tataatttta
     2761 ttgtttgctg tattatcact atatgtcatt gaagcctcaa taagttcttt taaagtgata
     2821 tcttttccta catatttttc taaaatagga gaataagcaa ctatatcatc tttgttaata
     2881 tgtacttttt tatttaactt attataaggt acttgttcta acaaaatagc actatttatc
     2941 gcttttgaag tcgaagcata ggcaaatctc ttatctgaat taaattttac ttccttacca
     3001 cttttagtat ttaaagcata aacaccaata tgagcattat attttttttc taaatcattt
     3061 aactctttgg catgtgaact gtttgaatta catgcactta aaactaaagc aattacaatt
     3121 aaaaatatta actttttcaa aataaaccct ccgatattac agttgtaatt tttataaatc
     3181 aataatatta caattgtaat attgatgtca attgtttatt ataaatattt gaaagaaggt
     3241 gtcaaaatgg ctaaattact aataatgagc gtagtaagct tttgttttat atttctattg
     3301 ttagtatttt ttaggtacat attaaaacgc tattttaatt atagtttaaa ttataaagtt
     3361 tggtatctaa ctatgcttgc tggattaatt ccttttatcc caattaaatt ctcttttata
     3421 aaatttaata atgtgaataa tcaagcgccc acagttgaaa gtaagtcaca cgatttgaac
     3481 cataacataa ataccaccaa acctattcaa gagttcacaa cagatatcca taagtttaat
     3541 tgggattcaa ttgataatat ctgcacagtt atttggatag ttttagttat tattttaagt
     3601 tttaaatttt tgaaagcctt attatatctt aaatatttaa agaaacagtc actttatcta
     3661 aacgaaaatg aaaaaaataa aatagatacg atacttttca accatcaata taaaaaaaat
     3721 attgtgattc gaaaagcaga ggctattcaa tctccaataa ctttttggta tgggaaatat
     3781 attattttga ttcctagttc atattttaaa agtgtaattg ataaaagact aaaatatata
     3841 atactgcatg aatatgctca tgctaaaaat agagatactt tacatttaat tatctttaat
     3901 atcttctcta ttattatgag ttacaatcca ttagtacata ttgtaaaaag aaagataatt
     3961 catgacaatg aagtagaagc cgatagattt gtccttaata atattaacaa aaatgaattt
     4021 aaaacttatg cggaatctat tatggactcc gtattaaaaa ccccattttc caacaaaaat
     4081 atattaagcc attcatttaa tggtaaaaaa tcattactca aaagtagatt aattaatata
     4141 aaagaagccg atctgaaaaa gcagtcaaaa ttaattctaa tttttatttg tatttttact
     4201 ttttttatca tgataattca aagccagttt ttaatggggc aatccttaac tgattataat
     4261 tataaaaagc ccttacaaag cgattaccaa attttagatg aaagtaaaaa ttttggttca
     4321 aatagtggat ctttcgttat gtacagcatg aagaaagaca aatactatat ttataatgaa
     4381 aaagaaagta gaaaaagata ttctcctgat tcaacttaca aaatttattt agcactgttt
     4441 ggacttgacc gtcatattat aagtgataaa aattcacgta tgtcatggaa tcataaacat
     4501 tatcttttcg agtcttggaa taaggaacaa gatttaaata cagcaatgca aaattcagtt
     4561 aattggtact tcgaacgtat tagtaatcaa ataccaaaga actatactgc ggctcaactc
     4621 aagcaattaa attatggtaa tgaaaatttg ggaagttata aaagctattg gatggaagat
     4681 agtttgaaaa tatctaatct tgaacaagta atagttttta aaaatatgat ggaacaaaat
     4741 aaccatttta gtaaaaaagc aaagaatcaa ttatcttctt cattattgat taagaaaaat
     4801 gaaaagtatg aactgtatgg gaaaacaggt acaggtatag taaacgggaa gtataataat
     4861 gggtggtttg taggttacgt aattacaaat catgataagt attattttgc tacacattta
     4921 tcagatggaa acccgtctgg gaaaaatgct gaattaataa gtgaaaaaat attaaaagga
     4981 atgggtgttt taaatgacca ataagcaagt tgaaatatct atggctgaat gggatgttat
     5041 gaatataata tgggataaaa aatcagtatc agctaatgaa attgtagttg aaattcaaaa
     5101 atataaagaa gttagtgata aaacgattag aacattaatc acaagactat ataaaaaaga
     5161 gattataaaa cgatacaaat cagaaaatat ttatttttac tcatcaaata ttaaagaaga
     5221 cgatattaaa atgaaaactg ctaaaacctt tcttaataaa ctgtatggag gggatatgaa
     5281 aagtttagtg ctgaattttg cgaaaaatga agaattaaat aacaaagaaa ttgaagaatt
     5341 gcgagacatt ttaaatgata ttagtaaaaa gtaattagag gtatgtttca aacataatac
     5401 atatgtaaac catacttgac tcttattaaa ttagttttgt taatgacatt taaaatatat
     5461 tattaaagat gagagtgtcc agaaaataac ccattttctg tacactcttg ttttttgtct
     5521 aaataagtaa gtattacata gaattatgtg ttcaaaaacc caacaaaaaa tctatgttca
     5581 caaaagacct cgtaatcaag ttatggtaca ctaaaggtgt atataggagg tctgtatttt
     5641 gaaaataggt tatgcaagag tttcaactgg attacaaaat ttgaatttac aggaggatcg
     5701 attaaataca tatggttgtg aaaagatatt taatgaccat atgagtggtt caaaaagtaa
     5761 gcgacctggt ttagataaag caattgaatt tgcgaggtca ggagatacga ttgttgtttg
     5821 gagattagat agactaggac ggaacatgga ggatttaatc acgttagtta atgaacttaa
     5881 cgaacgaggt gtgagttttc atagtttaga agaaaatata acgatggata aatcaagttc
     5941 tacaggacaa ttattatttc atttattcgc agcatttgca gagtttgaac gtaatttaat
     6001 tttagagcga tcatcagccg gtcgaattgc agcacgtgct agaggccgat atggaggtag
     6061 acctgaaaaa ttaaataaac aagatttaaa cttacttaag acactttatg ataatggtac
     6121 gccaattaaa acaattgcag agcagtggca agtttcacgt acaactattt atcgttatct
     6181 caataaatta gaggcaaaag agaataaaaa caagtagagc aactaatcta gcttactata
     6241 atgaatctac taaataattt tatatatcat taacatacaa agactttgat tcaatgtcac
     6301 tcgatgtaaa agagacgttc tgacagcgtc taaaatctca taatattttc ctgttatgag
     6361 attttaataa taacacaaga aaaaagcgta caaattcgta cgctttttta atataatacc
     6421 tgtttcacta atgaacgttt taagtatctt catcattaac tgaaactaca gtaataatac
     6481 tcgtcaacaa tattagtata gttaaaatag aaagacttag gactggcata accgaactaa
     6541 aattatattt gaaatataaa agtgctaatg aagagagaaa tgtaaaaata ataaatacca
     6601 aagaccattt ttctaattta ttcattggct tcacctcttt gaacttttat ataatcatag
     6661 gagatatttt tactacgatt tataaaaaat aagaagataa tcattattaa agaaacgatt
     6721 aatataatgt tgaatcctat agtatcacct aattcgttta cacctttgcc taatatcgga
     6781 gatacaaagc tttgtattaa taatgaaaga gatgcccata tagtaaatga ggttctgttg
     6841 caaagttgaa tttatagtat aattttaaca
//
LOCUS       DAKTLH010000357         6633 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.guided.140, whole genome shotgun sequence.
ACCESSION   DAKTLH010000357 DAKTLH010000000
VERSION     DAKTLH010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.guided.140"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            458..1924
                     /gene="msr(A)"
                     /locus_tag="O0394_002942"
     CDS             458..1924
                     /gene="msr(A)"
                     /locus_tag="O0394_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HCY0500921.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
     gene            2023..2922
                     /locus_tag="O0394_002943"
     CDS             2023..2922
                     /locus_tag="O0394_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HCY0500922.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            3408..4016
                     /locus_tag="O0394_002944"
     CDS             3408..4016
                     /locus_tag="O0394_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0500923.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            4262..4600
                     /locus_tag="O0394_002945"
     CDS             4262..4600
                     /locus_tag="O0394_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500924.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            4605..4790
                     /locus_tag="O0394_002946"
     CDS             4605..4790
                     /locus_tag="O0394_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0500925.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(5017..5760)
                     /locus_tag="O0394_002947"
     CDS             complement(5017..5760)
                     /locus_tag="O0394_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCY0500926.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            6186..>6633
                     /locus_tag="O0394_002948"
     CDS             6186..>6633
                     /locus_tag="O0394_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCY0500927.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENR"
BASE COUNT         2523 a          946 c         1023 g         2141 t
ORIGIN      
        1 tgttaaaatt atattataaa ttcaactttg caacagaacc gtattttatg agtacactct
       61 aaattaagga gatgtataat caatctaaaa ttgtattttt atacttttgt tatataatct
      121 ttctacttag agatatattg taaaaaaacc aacaaaatgt tatattaaca ttactatttt
      181 atcgatgaag gaggattaaa atgacagctt ctatgagact caaataaacc tttttgaata
      241 caaaactaaa ttaattcaaa aaggcttgtc cgcaatacac actcatagga tgacttatgt
      301 cattttttat ccttcgatag gaataataaa cgctttcaaa tactaaaaca tcataataaa
      361 tttttaagtg tttgaaagtg taatgtgaat gagacgggag tagcctgtct catttttttg
      421 cgtacacgtc ctttaaatat acaaggagtg tataaatatg gaacaatata caattaaatt
      481 taaccaaatc aatcataaat tgacagattt acgatcactt aacatcggtc atctttacgc
      541 ttaccaattt gaaaaaatag cacttattgg gggtaatggt actggcaaaa ccacattact
      601 aaatatgatt gctcaaaaaa caaaaccaga atctggaaca gttgaaacgg ttggcgaaat
      661 tcaatatttt gaacagctta acatggatgt ggaaaatgat tttaacacgt tagacggtag
      721 tttaatgagt gaactccata tacctatgca tacaaccgac agtatgagtg gtggtgaaaa
      781 agcaaaatat aaattagcta atgtcatatc aaattatagt ccgatattac ttttagatga
      841 acctacaaat cacttggata aaattggtaa agattatctg aaaaatattt taaaatatta
      901 ctatggtact ttaattatag taagtcatga tagagcactt atagaccaaa ttgctgacac
      961 aatttgggat atacaagaag atggcacaat aagagtgttt aaaggtaatt acacacagta
     1021 tcaaaatcaa tatgaacaag aacagttaga acaacaacgt caatatgaac agtatataag
     1081 tgaaaaacaa agattgtccc aagccagtaa agctaaacga aatcaagcgc aacaaatggc
     1141 acaagcatca tcaaaacaaa aaaacaaaag tatagcacca gatcgtttaa gtgcatcaaa
     1201 acaaaaaggc acggttgaga aggctgctca aaaacaagct aagcatattg aaaaaagaat
     1261 ggaacatttg gaagaagttg aaaaaccaca aagttatcat gaattcaatt tcccacaaaa
     1321 taaaatttat gatatccata ataattatcc aatcattgca caaaatctaa cattggttaa
     1381 aggaagtcaa aaactgctaa cacaagtacg attccaaata ccatatggca aaaatatagc
     1441 gctcgtaggt gcaaatggtg taggtaagac aactttactt gaagctattt accaccaaat
     1501 agagggaatt gattgttctc ctaaagtgca aatggcatac tatcgtcaac ttgcttatga
     1561 agacatgcgt gacgtttcat tattgcaata tttaatggat gaaacggatt catcagaatc
     1621 attcagtaga gctattttaa ataacttggg tttaaatgaa gcacttgatc gttcttgtaa
     1681 tgttttgagt ggtggggaaa gaacgaaatt atcgttagca gtattatttt caacgaaagc
     1741 gaatatgtta attttggatg aaccaactaa ttttttagat attaaaacat tagaagcatt
     1801 agaaatgttt atgaataaat atcctggaat cattttgttt acatcacatg atacaaggtt
     1861 tgttaaacat gtatcagata aaaaatggga attaacagga caatctcttc atgatataac
     1921 ttaaaaacta tacactaatt tatattgaat attaggttca tttatatcaa tataaaaaat
     1981 acgttcacaa gaaaaatata atagaaataa ggatccaata aaatgactcg acataatgaa
     2041 attattaaat gtgcagaaaa atatcaatta cacatccaac ctcaaacaat ctcattgaat
     2101 gaatcgggac ttgattttca agttgcattt ggaaaagata aacatggagt agaatgggtt
     2161 ttgagactac caagaagacc tgacgtttat aaacgaacaa aacccgaaaa acaaacggta
     2221 gacttcttac agaagaatgt ttcatttgaa ataccgaagt ggaaagtaca tgcaaaagac
     2281 cttattgctt acccaaaact tacaggtaaa cccgcagcca caatagatcc agaaatacaa
     2341 aattatgtat gggaaattga acacaaacca ttaccagaaa actttattaa cacattagct
     2401 gaaacactcg tagatttaca caacatacca gaagaaaaca ttaacgttca gcatataaat
     2461 atcaaaacca tacaagaaat aaaaaatgac tttcaaagaa gaatgaataa agttaaagaa
     2521 acttatggcg tatcagatga attatggaac agatggaaac aatggttaga aaacgacgaa
     2581 ctatggcctc gacatgcgac catgatacat ggggacttac atccaggaca tataatggta
     2641 gataaccaag caaacgtcac aggtctcata gactggactg aagcaaccca ctccgaccca
     2701 tcaatggact ttataggaca ccatcgtgta ttcgacgacg aaggattaga gcaacttata
     2761 acagcatacg gtaaagctgg aggtgaaata tggccacgaa tgaaagagca tataatagaa
     2821 ctcaatgcag tattcccaat gtttatcgct gagtttgcta tggaatcagg agaatcggcg
     2881 tatgaaacga tggcattgaa agagttaggt atgaaagagt agaacttata ttaaaagagg
     2941 cccattttaa agacgaaggc aatcctgatg aaattttaaa actgcgttta attagtgatg
     3001 gtctttggtt ttctataatg tattaaatat ctggacatgg aacaaatgca acaaatggaa
     3061 agaatcgttt tcgaaatttc taactcctta aatcaggagg ataaataaac ggtcattttc
     3121 attgaaagca cttatataaa tttcatcaat tttaatacct accgtaaata catttcataa
     3181 gtattaaaca gatgattaaa atttttagtt aatttaaata tttatgatag agatgacctt
     3241 caaatgaacg gttctgttac aaagaaaaaa tctatattta tttttagtaa gttttaatta
     3301 tagatatgta cactttgagt acaccctaac catacaaaaa gaattgttga aattataaca
     3361 attaaaacgt atgattaggg tgtatattta ttatggagtg aagatgaatg attataggtt
     3421 atgcgagagt atcatcgata gatcaaaatt tagaaagaca attggataat ttaaagacgt
     3481 ttggtgtgga gaaaatcttt acagagaaac agtcgggaaa gtcagtggaa aatagacctg
     3541 tatttcaaga agcactcaat tttgtcagaa tgggagatcg attcgttgtg gaatctattg
     3601 atcgtttagg acgtaactat gatgaaatta ttgaaactgt gaattattta aaagaaaaag
     3661 acgttcagtt aatgattacc agcttaccta tgatgaatga agtaattggc aatccattac
     3721 tggataagtt tatgaaagat ttaatcattc aaatattagc aatggtttca gaacaagaga
     3781 gaaatgaaag taaacgtcga caagcgcaag ggattcaagt tgcgaaggaa aaaggcgtat
     3841 ataaagggcg ccctttactt tattcaccaa atgctaaaga cccacagaaa cgtgttattt
     3901 atcatcgcgt ggtggaaatg ttagaagaag gcaaagcgat tagtaagatt gcgaaagaag
     3961 ttaatatcac aagacaaaca gtttatagaa ttaaacatga taagggatta tcttgataaa
     4021 ttgattgtca atgcaaatga gcgtctctca cagtgtctca aattccataa catattattg
     4081 aattgagatt ttaataatta tacaagaaaa aagagtacga gttcgcagct ttctatatag
     4141 taattgtttt actaataagg tgttttaaaa tatattataa tgtaaaaagt atttgacatt
     4201 tgtatcaaaa atatttacac tcataaatgt caaatacttt ttacatttat gaggtgttat
     4261 aatgaattta attcctttta ttattatttt aattttttct tgtttatgga cggctataac
     4321 taacaaatat ttaccttcag aaacattaga taaaaaagga caacaagatc gatacgatga
     4381 aagacaacgt aaaatgttta ttgaaatact ggctaaaagt tttatttgga ttgtatattg
     4441 tatgttattt acactagtgc ttaaatttac aggtttgtca ccaagtattg aaggatcttg
     4501 gttttctcaa tatccagaaa ttttctttat aattgtagca ctatttctta tgctgtttaa
     4561 ttactatact acaaataaaa aatatacatc taaaggttaa attaatgaaa aatagaatta
     4621 gagaatatag aaaacaaaaa ggaatttctc aagaaacttt aagtaaagaa ttaaaagtgt
     4681 ctaggcaaac tattaatgca atagaaaaca ataaatatga tcctacattg acattagcat
     4741 ttaagttggc aagattgttt gatacaactg tggatgaatt attttcttaa ccattcattt
     4801 atcacaaaaa gagagtgcga attcgcactc tctttttgtg tagtagtact attaaatttt
     4861 gtctcaataa cgaacgttta taacgcaatc atgatgagag tattaataca gttaattatt
     4921 tgaaatactt tagtttacat aatcgatgat taccagaagt tctcactata taaaagacac
     4981 taaaatttta taacttttag tgcctaatca attattttag ctaccaaaca aacgactcca
     5041 aaaccccttt ttaggttccg tttcttcact cttttcttct atatcactat cattgtcatt
     5101 tagattagct ggaacgtctt ctttttttgc ttcttcacct tgtgtacttt cgtcgctttg
     5161 acttccagat atatccttat attgaatctc tgtttcttta gtttcttcct gatctctgtt
     5221 aatatttaca ggattagtag tatatgtttt ttcttgtgca tcaacgtttt gtctagtgtt
     5281 agtagatgta tcaaagggat aattaaggtg tttttctttt tctaattgat tttctaattt
     5341 ttgaatcttt ttattactct ctaatgccag aacttgctga ttttcaagca attttgatct
     5401 ttcttgcaac tcttttctgt aaaattcaat tgttgtttca tgacgacttt cttgtttttc
     5461 aatttgatta tttaaagtat caatctgatt tttaaggatt tctactattt gagcatcgct
     5521 tttcgattca aaattaatat tatccgaccc ttttttttgt ttgtcttcag tagtactttc
     5581 aaaaccatat ttctttttat tttcatttac tctttgaatg attttttcta tatcggtatc
     5641 ttctttaata aacgaagtgt tttcctgttt tattgtctct atattcaatc gcttaatatt
     5701 attaaaaatc gtttgcttac taacccctaa ttcttcactc aactttttca cacttttcat
     5761 atcatcaacc ctttataaaa tgtttataaa cccttgataa aatattataa gcttataatg
     5821 cttaataata aagtgattaa taaataaaaa taaacatcca tcaacctttt tatcaagggt
     5881 ttatgaaagg tttatgatat ttatcaaggg tttataaacc cttgataaag ctatattttc
     5941 ccttttataa gtttatttaa agtgctataa taaacttaat ataacaaaaa ccccaactac
     6001 tggtaatagt cggggtttgg taataaaacg tttagaaact gtttatttat ataattttcg
     6061 atacttgtat tatataatga ataatatctt agtgcaagca cctaaatcat taaataaatg
     6121 taaagctaaa taaaaacgtt ttattacctc ttaccaaact aaaatatgga aagaggtttt
     6181 ttgtcatgca aaatcaatat tttacagtgc aagaaaatta caaagaaaga ttttatcaaa
     6241 tccctaaagt attctttacc agtgaaaatt ataaaaatct aactaatgat atgaaaatag
     6301 cctatgctat cttacgtgat cgcttaaatt tatctattaa aaatagctgg gttgatgaag
     6361 atggtaacat ttactttgtt tattcaaatg aaaagctaat ggaaatctta aattgtaaaa
     6421 aagaaaagtt aacaaaaata aaaaaagggt tagaaaacga tggtctactc attcaaaaac
     6481 gacgtggttt aaataaacca aatattctat atttaatgaa acctattgtc acagaacgtg
     6541 atatttacaa aatagaaaaa gaagaaaacg atgttgaacc ttacggcgag aaggaagttc
     6601 gaaaatcgaa cgtccagaag ttcgaaaatc gaa
//
LOCUS       DAKTLH010000358         5990 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.guided.152, whole genome shotgun sequence.
ACCESSION   DAKTLH010000358 DAKTLH010000000
VERSION     DAKTLH010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.guided.152"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            complement(90..794)
                     /locus_tag="O0394_002949"
     CDS             complement(90..794)
                     /locus_tag="O0394_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HCY0500928.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            1039..1233
                     /locus_tag="O0394_002950"
     CDS             1039..1233
                     /locus_tag="O0394_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HCY0500929.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            1245..1496
                     /locus_tag="O0394_002951"
     CDS             1245..1496
                     /locus_tag="O0394_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HCY0500930.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            1534..2658
                     /locus_tag="O0394_002952"
     CDS             1534..2658
                     /locus_tag="O0394_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HCY0500931.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIQEVMDGVLEEVLTTYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPVVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            2674..3111
                     /locus_tag="O0394_002953"
     CDS             2674..3111
                     /locus_tag="O0394_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCY0500932.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            3535..4491
                     /locus_tag="O0394_002954"
     CDS             3535..4491
                     /locus_tag="O0394_002954"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCY0500933.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            4691..5170
                     /locus_tag="O0394_002955"
     CDS             4691..5170
                     /locus_tag="O0394_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCY0500934.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            5185..>5990
                     /gene="fakB2"
                     /locus_tag="O0394_002956"
     CDS             5185..>5990
                     /gene="fakB2"
                     /locus_tag="O0394_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCY0500935.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTG"
BASE COUNT         2213 a          894 c         1040 g         1843 t
ORIGIN      
        1 aagttggggt gggacgacga aataaatttt gcgaaaatat tatttctgtc ccactccctt
       61 aaaacttatt cttttgtgta gtaagtgcgt taatagcctt gatctaactt atcaatctta
      121 cctttacgat aaaatgattt agcaatatat ccaaatggta cattgaaaac tgttgaagct
      181 aattttaata cgtacgttgt aataaatatt tcaaatacaa ctgtaccagg taaacttccg
      241 ataaatgcga tagctacaaa taaagctgta tcaattattg agcttaaaaa tgtactacca
      301 tatgcacgga tgaaaaacgt tttatcagaa ctaaatactt ttttaattag tgaaaagata
      361 aatacatcaa tatgttgacc aataatatat gcgacgattg agcctaaagc aatgcgtggc
      421 acaacatcaa agattgcgtg taatgctttt tgtgccatat cttctggtgc aggaataaaa
      481 tgtaatgaca attgcataac aataatcata attaatgttg atgaaaagcc taaccaaact
      541 gctctttttg caactctacg cccataaata tcgtttaata tatctgttgc taaataaata
      601 gaagcaaaca tgacattacc taaagttgct gaaataccaa agatttctac agttttaatc
      661 acttgtatgt tggcaatgat tgtgccaatt gcaacccatg caattaaacc ttgtttacca
      721 aaaaagcgat acataagtac cataagcacg aacgttgcaa taaacgtaac tagtcctaaa
      781 atttcattat acatattaaa atgtcctcct aaattttgat catgcgggtg tttagaaacc
      841 gctcaataaa taactaactc attttactga aaaatcgttt gcgatgcaat agcatttgaa
      901 tataaataca ttttacgtat agaattgtaa aaggtttcat tcaaatctta gggtcaaaaa
      961 tgttataata tttttatgtc aaatttaaaa cagtaacact tatttacaag gttgcaatat
     1021 tttgaagtaa taaaggaagt gtcgcgtatt ttaacttttt cagagcaaaa tgcactcgcg
     1081 aaaatagatg atttaatgaa tacttattgc aatcaatgtc caatcaaaac tcgtctgcgt
     1141 aaattagagg ggaaaacgaa ggcgcatcat ttttgtatca atgagtgttc aatagggaaa
     1201 gaaataaaac aattaggaaa tgaacttcaa taggaggaag tcaaatgaaa attatatcta
     1261 tatcagaaac accgaaccac aacacaatga agattacact tagtgaaagc agagaaggta
     1321 tgacatcaga tacgtatact aaagttgatg attcacagcc agcatttatt aatgacatct
     1381 taaaggttga aggcgttaaa tcaattttcc atgttatgga ctttatttca gtagataaag
     1441 aaaatgacgc aaattgggaa acagtattgc caaaagtaga ggctgtattc gaataaattt
     1501 ttcatcaact agtattcggg gggaataaag tatatggaaa ttttacgtat agagccaaca
     1561 ccaagtccaa atacaatgaa agttgttttg tcatatacaa gagaagacaa gttatctaat
     1621 acttataaaa aagtagaaga aacacaacca agatttataa atcagttgtt atctatagat
     1681 ggtatcactt ccatttttca tgtcatgaac ttcttagctg ttgataaggc accaaaagct
     1741 gattgggaag tcatattacc tgatattaaa gctgcttttt ctgatgcgaa taaggtttta
     1801 gaatctgtaa atgaacctca aattgacaat cattttggtg aaattaaagc tgaattatta
     1861 acttttaagg gtataccgta tcaaattaag ctaacttctg ctgaccaaga attaagagaa
     1921 caattaccac aaacatatgt tgaccatatg actcaagcgc aaacagcaca tgacaatatt
     1981 gtttttatgc gtaaatggct agatttagga aatcgctatg gaaatattca agaagtaatg
     2041 gatggtgtcc tagaagaagt gctaactacc tatccagaat cacagttacc cgtattggta
     2101 aaacatgctt tagaagaaaa tcacgcaact aataattatc atttctatcg acatgtctct
     2161 ttggatgaat atcatgcaac tgataattgg aagactcgat tacgaatgtt aaaccatttt
     2221 ccaaagccga cttttgaaga tataccgctg cttgatttag ctttatctga tgaaaaagta
     2281 ccggttagac gtcaagcgat tgtattatta ggtatgattg aaagtaaaga aattttaccg
     2341 tatttatata aggggcttcg tgataaaagt cctgttgtaa gaagaacagc aggggattgc
     2401 ataagcgatt tagggtatcc agaggcacta ccagaaatgg tgctactatt agatgatcca
     2461 cagaaaatcg ttaggtggcg tgctgctatg tttatctttg atgaaggtaa tgcagagcag
     2521 cttcccgcac taaaagccca tattaatgac aatgcgtttg aagttaaatt acaaattgaa
     2581 atggccatat ctcgtattga aaacggagat gaagcacttg gttcagtatg gaaacaaatg
     2641 gcaaacagaa caatttaatt ggaggaatta aatatgaatg catatgatgc ttatatgaaa
     2701 gaaattgcgc aacaaatgcg tggcgaatta actcaaaatg gttttacaag tttagaaacg
     2761 agcgaagcag tatcggagta tatgaaccaa gtaaatgctg atgacactac ttttgtagtt
     2821 attaactcta catgcggctg tgcagctgga ttagcaagac cagctgcagt agcagttgca
     2881 acacaaaatg aacatagacc tacaaataca gttacagttt ttgctgggca agataaagaa
     2941 gcaactgcta caatgcgaga attcattcag caagcaccat ctagtccttc gtatgcttta
     3001 ttcaaaggtc aagatttagt ttattttatg cctagagaat ttatcgaagg tagagatatt
     3061 aatgacattg caatggactt aaaggatgcc tttgacgaaa attgtaaata gtacacataa
     3121 ataaatataa aggttaacac attttataat attaaaaatg gtgtctgtca ttgaaaatag
     3181 agaatatagt tgtattctat ttgttaaata aagtccgttt ttaccaacta tattttctag
     3241 aaatttaact gttttaatag gacatcaaac ataatattca aatcatgtgt taacctcttt
     3301 tttaaaattt tttagcatta aagttataga tttgggtaaa caattaccaa ttggaaacat
     3361 atatcacgtt acgatggggt aggtacttaa tcagcatttt ataaataaag taacggaatt
     3421 catgatatta atatcatatt cctaaaatga gtgataacaa aatgctacat aaagttaagt
     3481 tatatcaaac taaatataca tactataaat aatgaaaatg aggtgttatc gcatatgttg
     3541 aattcatttg atgcagcata tcacagtctt tgtgaagaag ttttagaaat aggaaataca
     3601 cgaaatgatc gcacaaatac aggtacgatt tcgaaatttg gtcatcaact tcgctttgac
     3661 ttatctaaag gatttccact attaacgaca aagaaagttt cttttaaatt agtagcaacc
     3721 gaattattat ggttcattaa aggagataca aacatccaat acttattaaa atataataat
     3781 aatatatgga acgaatgggc ttttgaaaat tatatcaaat cagacgagta taaaggtcca
     3841 gatatgacag atttcgggca tcgtgcattg agtgatcctg aatttaacga acaatataaa
     3901 gaacaaatga aacaatttaa gcaacgtatt cttgaagatg atacatttgc gaagcaattc
     3961 ggggatttag gaaatgttta tggtaaacaa tggcgagatt gggttgataa agatggtaat
     4021 cattttgatc aacttaaaac agtaattgaa caaattaagc ataatccaga ttcaaggcga
     4081 cacatcgtat ctgcatggaa tccaacagaa attgatacaa tggcacttcc gccttgtcat
     4141 accatgttcc agttttatgt ccaagatggt aagttaagtt gccagttata ccaacgtagc
     4201 gcagatatct ttttaggtgt gccatttaat atcgcaagct acgctttatt gacacacctt
     4261 attgccaaag aatgtggact tgaagtgggt gaatttgtgc atacatttgg agatgcacat
     4321 atttattcaa atcatattga tgcgattcaa acacaattag cacgtgaaag cttcaatcct
     4381 ccaacattaa aaattaacag tgacaagtct attttcgaca taaattatga agatttggaa
     4441 attgttgact atgaatcaca tccagcaata aaagctccaa tagcagtgta gtcattgcat
     4501 agttagctaa ccatatagac atcaaaatga catcatagta ttttcaagtg caaaaaagta
     4561 cttttttgtg ttaaacgttt tcataaatta tgcaaaatca ttatttctat cacactttat
     4621 gataaaaatt gtgttaaatt aaagataact tagtaataaa aaatgaaatg atagaagaag
     4681 gaggataatt atgactttat ccattctagt tgcacatgac ttgcaacgag taattggttt
     4741 tgaaaatcaa ttaccttggc acctaccaaa tgatttgaag catgttaaaa aattatcaac
     4801 aggtcatact ttagtaatgg gtcgtaagac atttgaatcg attggtaaac cactaccgaa
     4861 tcgtcgaaat gttgtactta cttcagatac aagtttcaac gtagagggcg ttgatgtaat
     4921 tcactctatt gaagatattt accaactacc gggccatgtt ttcatatttg gagggcaaac
     4981 attatttgaa gaaatgattg ataaagtgga cgacatgtat attactgtta ttgaaggtaa
     5041 attccgtggt gatacgttct ttccacctta tacatttgaa gactgggaag ttgcctcttc
     5101 agttgaaggt aaactagatg agaaaaatac aattccacat acctttctac atttaattcg
     5161 taaaaaataa gggggaaaac gaccatgaca aaacagatta tagtaacaga ctcaacatcc
     5221 gatttatcta aagaatactt agaagcaaac aacattcatg taattccttt aagtttaact
     5281 attgaaggag cttcatacgt tgaccaagta gatattacat cagaagaatt tattaatcat
     5341 attgaaaatg atgaagatgt aaagacaagt cagccagcca taggtgaatt tatatctgct
     5401 tatgaagaac taggaaaaga tggctctgaa atcataagta ttcatctttc ttcaggatta
     5461 agtggtacat ataacactgc ttaccaagca agtcaaatgg tagatgctaa tgtaactgtt
     5521 attgattcaa aatctatttc ttttggttta gggtatcaaa tacaacacct agtagagctt
     5581 gtaaaagaag gtgtctcaac ttctgaaata gttaaaaagt taaatcattt aagagaaaac
     5641 attaaattat ttgtagttat agggcaattg aatcaattaa ttaaaggtgg cagaattagt
     5701 aaaacaaaag gtttgattgg taatcttatg aaaattaaac caattggtac actagatgat
     5761 ggtcgcttag agcttgtgca caatgcgaga actcaaaatt ctagtatcca atacttgaaa
     5821 aaggaaattg ctgaatttat aggagatcat gaaatcaaat ccattggtgt cgcacatgct
     5881 aacgtcattg aatatgttga taaattgaag aaagttttta atgaagcttt tcatgtgaat
     5941 aattacgata taaatgtaac tacaccagtt atttctgcac atactggtca
//
LOCUS       DAKTLH010000359         5720 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.guided.156, whole genome shotgun sequence.
ACCESSION   DAKTLH010000359 DAKTLH010000000
VERSION     DAKTLH010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.guided.156"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            <1..447
                     /locus_tag="O0394_002957"
     CDS             <1..447
                     /locus_tag="O0394_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HCY0500936.1"
                     /translation="AILVLIIPLNMLISRQYLLIVNKIRLYNASITIGAVINLVLCII
                     LIYFYGIYGAAIARLITEFFLLIWRFIDITKINVKLNIVSTIQCVIAAVMMFIVLGVV
                     NHYLPPTMYATLLLIAIGIVVYLLLMMTMKNQYVWQILRHLRHKTI"
     gene            715..1314
                     /locus_tag="O0394_002958"
     CDS             715..1314
                     /locus_tag="O0394_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCY0500937.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            1682..2407
                     /gene="sasD"
                     /locus_tag="O0394_002959"
     CDS             1682..2407
                     /gene="sasD"
                     /locus_tag="O0394_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HCY0500938.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(2599..3354)
                     /locus_tag="O0394_002960"
     CDS             complement(2599..3354)
                     /locus_tag="O0394_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0500939.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            3590..4297
                     /gene="deoD"
                     /locus_tag="O0394_002961"
     CDS             3590..4297
                     /gene="deoD"
                     /locus_tag="O0394_002961"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCY0500940.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            4304..5656
                     /gene="tet(38)"
                     /locus_tag="O0394_002962"
     CDS             4304..5656
                     /gene="tet(38)"
                     /locus_tag="O0394_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCY0500941.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
BASE COUNT         1952 a          852 c          996 g         1920 t
ORIGIN      
        1 gcgatacttg tattaatcat tcctttaaat atgttgataa gcaggcaata tttattaata
       61 gtgaataaaa taagattata taatgcgtca attactattg gtgcagtgat aaacctagta
      121 ttatgtatta ttttgatata tttttatgga atttacggtg ctgctattgc gcgtttaatt
      181 acagagtttt tcttgctcat ttggcgattt attgatatta ctaaaatcaa tgtgaagttg
      241 aatattgtaa gtacgattca atgtgtcatt gctgctgtta tgatgtttat tgtgcttggt
      301 gtggtcaatc attatttgcc ccctacaatg tacgctacgc tgctattaat tgcgattggt
      361 atagtagttt atcttttatt aatgatgact atgaaaaatc aatacgtatg gcaaatattg
      421 aggcatcttc gacataaaac aatttaagta ccggtaatgc tatactttag aaaattaaga
      481 ttaagaagaa aaggcaattt cttattgaaa aatggaagtt gtctttttta attctcttta
      541 aaagcgggaa acaaaagcag ttaaatgcct ttttgcattc aatattaaat attatatcaa
      601 tttcgaatat ttaaatttta tataattgga tataacaaat aaataataat tattgcaaaa
      661 cacacccgaa attaattatt ataaaagtat attcataaaa ggaggaatat acttatggca
      721 tttaaattac caaatttacc atatgcatat gatgcattgg aaccatatat agatcaaaga
      781 acaatggagt ttcatcacga caaacatcac aatacgtacg tgacgaaatt aaacgcaaca
      841 gttgaaggaa cagagttaga gcatcaatca ctagcggata tgattgctaa cttagacaag
      901 gtaccggaag cgatgaggat gtcagtccgt aataatggcg gtggtcattt taaccattca
      961 ttattctggg aaatactatc acctaattct gaagaaaaag gtggcgtaat agatgacatc
     1021 aaagcgcagt ggggcacttt agatgaattt aaaaatgaat ttgcaaataa agcaacaaca
     1081 ttatttggat caggttggac ttggttagtt gttaatgatg gcaaattaga aattgtgaca
     1141 acgccaaacc aagataatcc attaacagaa ggcaaaacac caatcttact atttgatgtt
     1201 tgggagcatg cctactatct gaaatatcaa aataaacgtc cagactatat gactgcattt
     1261 tggaacattg ttaactggaa aaaagttgat gaattatacc aagcagcaaa ataatataac
     1321 ttaatataat tgaggtggag catctacaag gtgttccact tttttgtact taatatcttt
     1381 acttatttgt ttttgtgtaa tgggatagca cgtaaaagtg gaaaaataat aagataaatt
     1441 tcaactcgaa ataccttatt attgttgact ataacgatga cctaaatatt atatacatta
     1501 gacaacacta ttaattaatc aacatattga cattaaataa attgacaaaa taagtaatta
     1561 ttgtgaatct aaagtgaaat ttttataaaa aaatgtaatg attcaaaaat tttgttgcat
     1621 ttcttttgta atcgtatgat aatgtaaatg taatcaaatt gtaatataag gggacaagac
     1681 aatgaaaaaa ttagcaacag taggttcttt aattgtaaca agcactttag tattctcaag
     1741 tatgcctttt caaaatgcgc atgccgacac aacttcaatg aatgtgtcga ataaacaaag
     1801 ccaaaatgta caaaatcatc gtccttatgg cggagtagta ccacaaggaa tgacgcaagc
     1861 acaatatact gaattagaga aagctttacc ccaattaagc gctggcagta atatgcaaga
     1921 ctataatatg aaattgtatg atgcgacgca aaatattgct gataaataca atgtgataat
     1981 tacaactaat gtaggggtat ttaaaccaca tgctgttaga gatatgaatg gccatgcgtt
     2041 acctttaaca aaagatggca atttttatca aacgaatgta gatgcaaatg gtgttaatca
     2101 tggtggtagt gaaatggtgc aaaataaaac aggtcatatg agtcaacaag gccatatgaa
     2161 tcagaacaca cacatgaacc aacagccaca catgcaacaa ggtcatatgc aatcatcaaa
     2221 ccatcaaatg atgagtccaa aagcaaatat gcattcatca aatcatcaaa tgaaccaaag
     2281 taacaaaaaa gttttaccag ctgctggtga aagtatgaca tcaagtattc ttactgcaag
     2341 tattgccgca ctactattag tatctgggtt attcttagca tttagacgac gttcaacaaa
     2401 taaataaaca taatacgatt aataatagaa aaatcgtgtg attatctgag cgagcctagg
     2461 acataaatca atgtcctagg ctcgctaatg ttatattggc agtagttgac tgaatgaaat
     2521 tgcgcttgta acaagctttt ccatttctta ccaactccct aaatagtcat caaaaaattt
     2581 cttatatttt aataattttt aataatccga ttgtcttata cgtgtcagtg ttaattcaga
     2641 tatttcctgt ggaatatacc acttattaat cataattgga taaggtgttt gtgcgtacag
     2701 tgtttcaata atcagccaac aatgtgtatc accatcaaac acgtgactat gatttttgaa
     2761 gtggggcgct ttggtaatag acatttttaa atctgattga tatgcattgc tataaatcgt
     2821 ttgctcaacg aatgtcttca tgtcgtcttc gttttgtgta ttcactttaa atgtgtcaat
     2881 gacatttaac ggtataaagg taaagcaaaa tgcatcagct tgcttagaat gattgtcctt
     2941 tttttgataa tagcgttcca ttgcaatgac ggcagaagga tggtttgcaa acaaatgatt
     3001 tgtatattca ctttctaaat caacacgata attaattgat gacatagata cgcgagctag
     3061 caatatttga tcaagtggat gcttaaattg atccatactt gaagcgtgtt gggcatttgt
     3121 ttgtggaata acaaagtgtc ccttccctct tgtactctct acgatgccat cttcggctaa
     3181 caattttata gcttggcgca aagtcatacg actgacatca aagcgcgcac aaagttcctt
     3241 ttcagtaggt aatgcatggc cactcggata ttttcctatt tgaatttctt tatataacgt
     3301 attataaatc gttaaaaatt ttggttgtgt ttgcgtcacg tagacaacct ccataaagtt
     3361 acttaatcac tctcatcata caataatttt tactcaaatt ggaaaaatta taaaaattaa
     3421 atatagatag gctttgaaaa ttagttttat acaaggttag tagctgtaac tgtaaaatgt
     3481 tcttaatatt gtcaaaatgt aatgcttgaa agcgctttta aaaaatatta ttatatacat
     3541 ggttagacaa atagacaaat cactatacaa atattgggag gaatatttta tgaaatcaac
     3601 accacacatt aaaccaatga atgacgtcga aattgcagaa acggttctat tgccaggaga
     3661 tccgttaaga gctaagttca ttgcagaaac ttatttggat gatgtggaac agttcaatac
     3721 agtgcgaaac atgtttggtt ttaccggaac atataaaggt aaaaaagttt ctgtcatggg
     3781 ttcaggtatg ggtatgccat ctattggcat ttactcttat gaattaattc atacatttgg
     3841 ttgtaaaaaa ttaattcgcg ttggctcttg tggcgcgatg caagaaaaca ttgatttata
     3901 tgatgtgatt attgcacaag gtgcctctac tgattcaaat tacgttcaac aatatcaatt
     3961 accaggtcat tttgcgccaa ttgcttctta tcaattatta gaaaaagcag ttgaaacagc
     4021 acgtgacaaa ggtgtacgtc atcatgtagg taatgtgtta tcaagtgata ttttctataa
     4081 cgcggataca acagcgagtg aacgttggat gcgtatgggt attttaggtg tagaaatgga
     4141 atcagctgca ttatacatga atgcaattta cgctggtgtc gaagcattag gtgtgttcac
     4201 agtgagcgat catttaattc atgaaacgtc aacaacacct gaggaaaggg aacgtgcatt
     4261 tacagatatg attgaaattg cactgtcatt ggtgtagatg attatgaatg ttgaatattc
     4321 taaaataaag aaagcagtac ctattttatt attcttattt gtattcagtt tggttataga
     4381 caactcattt aaattgattt ctgtagccat tgctgatgac ttaaacatat ctgtaacgac
     4441 agtaagttgg caagcgacat tagccggttt agtaattggt attggcgctg tagtatacgc
     4501 ttcattatct gatgccatta gtatacgcac actatttatt tatggcgtga tattaatcat
     4561 tatcggatca attattggtt acattttcca acatcaattc ccattacttt tagttggacg
     4621 tattattcaa actgccggtt tagctgctgc agagacatta tatgtgatat atgttgcaaa
     4681 gtatctttct aaagaggacc agaagactta ccttggctta agtacgagca gttattcctt
     4741 gtcattagtt atcggtacat tatcaggtgg atttatttct acgtatttac actggacaaa
     4801 tatgttttta attgcattaa tcgtagtatt tacgttgcca ttcctattta aattattacc
     4861 aaaagaaaat aatacgaata aagctcattt agattttgtt ggcttaattc tagtggcaac
     4921 tattgctaca acagtcatgc tgtttattac gaactttaat tggttatata tgattggtgc
     4981 cttaattgcg attatcgttt ttgcgctata tattaaaaat gcgcaacgtc cattagtaaa
     5041 taaatcattt ttccaaaata aacgttatgc ttcattttta tttatagtat ttgtaatgta
     5101 tgctatccaa ttgggttata tttttacgtt cccattcata atggagcaaa tttatcatct
     5161 gcaactagac acaacatcac tgttattagt accgggttat atagtagcag tcattgttgg
     5221 tgcactaagt ggtaaaatcg gcgaatatct gaattcaaaa caagcgatta tcacagcaat
     5281 tattttaata gcactgagct tgattttacc tgcatttgca gtaggtaatc acatttcaat
     5341 cttcgtcatt tctatgatat tctttgcagg tagctttgct ttaatgtatg cacctttact
     5401 taacgaagcc attaaaacaa tagatcttaa tatgacaggt gtggctattg gtttttataa
     5461 tttaattatt aatgtggcgg tatctgtagg tattgcgatt gctgcggctc taatcgattt
     5521 taaagcatta aatttcccag gcaatgatgc attaagttca catttcggta ttattttaat
     5581 tattttaggt ttaatgagta ttgtcggatt agttttattc gtcagcttaa atcgttggac
     5641 acaatctgaa aaataaatag atataaattc gcgagatata ttcgtattta tagtaaaatt
     5701 aaataaagag attatataac
//
LOCUS       DAKTLH010000360         3299 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.guided.198, whole genome shotgun sequence.
ACCESSION   DAKTLH010000360 DAKTLH010000000
VERSION     DAKTLH010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.guided.198"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            39..416
                     /locus_tag="O0394_002963"
                     /pseudo
     CDS             39..416
                     /locus_tag="O0394_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            413..1294
                     /locus_tag="O0394_002964"
                     /pseudo
     CDS             413..1294
                     /locus_tag="O0394_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1410..2339
                     /locus_tag="O0394_002965"
     CDS             1410..2339
                     /locus_tag="O0394_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0500942.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGA
                     GKTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLT
                     INAKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILD
                     EPTNGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEI
                     MYEDLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINR
                     LLVLNNIDVFQMRVIEDNLETYFEDLVGGGTIG"
     gene            2332..3069
                     /locus_tag="O0394_002966"
     CDS             2332..3069
                     /locus_tag="O0394_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0500943.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKV
                     PTPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLKNLLIIPISRESFL
                     VSKIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSP
                     PIIILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFL
                     LSLIIIIVTSIFSVIILFSYFKKTDIS"
     gene            3083..3286
                     /locus_tag="O0394_002967"
     CDS             3083..3286
                     /locus_tag="O0394_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0500944.1"
                     /translation="MGFIEQIKAFILGVVEGLTEFAPVSSTGHMIIVDDMWLNTADDL
                     GSASATTFKVVIQLGGSVAKLNL"
BASE COUNT         1201 a          433 c          587 g         1078 t
ORIGIN      
        1 attagtgatt agctatattt tttactttgc aacagaacca gccatttaat atgactgtta
       61 caattactaa gattcaagca ctattacgcc gcacgtatga tttcaccact caagttaata
      121 atttaacagt agatgattgc gagttactac ttgatgcagc aaagcttaaa tttaatgaag
      181 aaaccataga cctgacacac actgaattgc aaatcatgca tagcttattc cagaacaagg
      241 gcaaattcgt cagtcgtaat gcgttaatcg aaaaatgctg ggaatcagaa cacttcattg
      301 atgacaatac gcttgctgtc aacattacac gtttacgcaa aaagctacaa aaactaggac
      361 ttacagattt tatcgaaacg aagaaaaatg tcggatatcg tgtaggtgac gtatgaattt
      421 cttgaaatca atccaacgtg aaatttatat tgtcatcctt acttgtgctt tattctactt
      481 tatattattc atgtataatg tgccgatgca agcatatatg attggtctag gtattgtatt
      541 gtttgtttca atgatttact ggggtatcca ttacatgaac tttaaaaaag aataaacact
      601 acaggaacag aacgaagatt taagaatcga aaaccataaa cttcgtaatg actttattaa
      661 ctaccaaagt gagttagagt catatttttt aatgtgggtg catcaaatca agacaccgat
      721 tacagcatca aagttactgc ttgaagatcc tgatgaacac gtgatttcta acgtgcgtaa
      781 tgaagtctta caaattgata attacacgaa tcttgcactg aactatttga aactcatgaa
      841 ccaaaagaca tacatggtct ttatggaagt gagtgtagat gaattaattc gaccactgat
      901 taaacgttat gcgatacagt ttattcatca tcaaacaaag ttgcattatg agaaaagtga
      961 agcggttata ttaacagatg ccaaatgggc atcaattatg attgagcaaa tattaaacaa
     1021 tgcattaaaa tatgcacgtg gcaaagatgt gtggattacg tttgacgaaa atacaaagaa
     1081 attatcaatc cgagataacg gtatcggcat taatgcgagt gatttaccga aaatctttga
     1141 taaaggtttc tcgggctata acggtagaat aagtgagaaa tcaagcggta ttggtttata
     1201 tattgtcagc acaatctcaa agcgcttagg ccataaagta agtgctacat ctacattaaa
     1261 cgatggttca atattcacca ttcaatttaa ataactccaa tctttcattt ttgtaagatt
     1321 agcggctgtt tttgtaagaa tagataaagg cagccttttc taatcgcgcg taagataaag
     1381 gtaacaacaa actaagaaag gtgccagagg tgaaaaattt gaataatata attgaaataa
     1441 ataacttaac aaagcaatat ggaagtcaga tagcagttaa taatttgagt ataactgttg
     1501 aaagtggaca aatttatggt tttttaggaa gaaatggtgc aggcaaaaca actactataa
     1561 gaatgctttt aggcttaatt aaacctacga aaggtaatat taaaatattt gaaaaagagt
     1621 tcgaaagtaa taaaactgaa attttaaaaa atattggtgc tatggttgaa acgccaggtt
     1681 tttatagtaa tttgacagct aaagagaact taactataaa tgctaaaata ttaggtatac
     1741 aaaaagaaaa tgcaattgaa gaagtgttag aaatagtagg actagcttct gaaaaagtaa
     1801 aattgtttaa aaattattct ttaggtatga agcaacgttt agggattgcg agagctttat
     1861 tgcacaatcc cgaattattg atacttgatg agccgacgaa tggattagat cctattggca
     1921 taaaagaaat tagaaagttg attaaggatc tggctcaatc tagaaatata accattttaa
     1981 tttcaagtca tatattgtca gaaatagagc agttagtaga caaggtgggt attattcata
     2041 acggagtttt gttagaagaa attatgtatg aagatttaaa aaaaaagaac agacaattta
     2101 taagtatatc agtatcagat gaagaaaaag ttgcatgttt gttggaaact aacttcggaa
     2161 tttatgacta taaaattaaa gaaaatggcg aatttaaaat ttattcacat attgatagac
     2221 aacaagaaat taatagatta ttagtattaa acaatattga tgttttccag atgagagtga
     2281 tagaagataa tctagaaact tatttcgaag acttagtagg aggtggcaca attggataat
     2341 ctactccgta cagaatttat taagttaaaa agatcatata tgtttctaat aagtatttta
     2401 ggtacatgcg tagcaccttt tatggtggtt gtagcttctt ttattagtat gcggacgaaa
     2461 gtaccgactc ctaatattgt atttaaacag ttatttgaag aagtcagttt atatcatatg
     2521 ctaattattg gacctcctct atatggggtt ataatagcct atttgtttgg aagagaatat
     2581 atagaagata ctttaaaaaa cttattaatt atacctatat ctagagaaag ttttttagta
     2641 agtaaaataa tattattatt cttgtggatt caaacactat ctgttattgc atggttgcta
     2701 actttaatgc taggtatgct attgcatttt caagggttaa gccagagttt aataattgat
     2761 tcattcttta catttgttat tggcggagtg ttattatgga tattatctcc accaataatt
     2821 attttgtcga tatattttaa aaattatgtt cccacaattg ttttaaccat aataattact
     2881 ttgataaatt taatgactgc taattctgaa catagaggat tatttccttg ggctgcagtt
     2941 ggagacatat ctagaggaac attacctaat acttatccat ttctattatc tttaattatc
     3001 ataattgtta catctatatt cagtgtaatt atattattct catactttaa aaaaactgat
     3061 ataagttaaa aggaggagaa aaatgggttt tatagagcag attaaagcat ttattttagg
     3121 tgtagttgaa gggttgacag aatttgctcc agtatcatca acaggtcata tgattattgt
     3181 tgatgatatg tggttaaaca ctgctgacga tttaggaagt gcgtctgcaa ctacttttaa
     3241 ggtggtaata cagttaggag gttctgttgc aaagttgaat ttataatata attttaaca
//
LOCUS       DAKTLH010000361         3226 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.guided.200, whole genome shotgun sequence.
ACCESSION   DAKTLH010000361 DAKTLH010000000
VERSION     DAKTLH010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.guided.200"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            122..757
                     /locus_tag="O0394_002968"
                     /pseudo
     CDS             122..757
                     /locus_tag="O0394_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            754..1284
                     /gene="sat4"
                     /locus_tag="O0394_002969"
     CDS             754..1284
                     /gene="sat4"
                     /locus_tag="O0394_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HCY0500945.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFEKAVFWYLRF"
     gene            1377..2171
                     /locus_tag="O0394_002970"
                     /note="aph(3')-IIIa"
     CDS             1377..2171
                     /locus_tag="O0394_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCY0500946.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2447..3018
                     /locus_tag="O0394_002971"
                     /pseudo
     CDS             2447..3018
                     /locus_tag="O0394_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         1078 a          508 c          736 g          904 t
ORIGIN      
        1 attagtgatt agctatattt tttactttgc aacagaacca tcatttttat taaaatactc
       61 ttttgtaaaa tctttctgct cttcaatgta tttaatttct ttacctctaa atctattata
      121 gctatttgat gattacaata aaattgatct taccttattg cccttggaag agttagataa
      181 ttacctaaag ggcgataaat taataaaggt tctaattgat aaagattgta gaattaaaag
      241 ggacatagtt ccgactgata tagattatca tgtaagaaag ccaagcgcaa gggagtatga
      301 tgattgctgc aatgaatttt ggaatgtaac accttatgtt attaaaggat tgtgccgtaa
      361 ggaaatttta tttgctattg atcattttaa tcagattgtt cgccatgagc tgctgagaat
      421 gatatcatgg aaggtcggca tcgaaacagg ctttaaatga agtgtaggca agaactataa
      481 gtttattgaa aggtatatat ccgaggattt gtgggagaaa cttttgtcca cctaccggat
      541 ggattcctat gaaaacatat gggaagcatt atttctatgc catcaattgt tcagggcggt
      601 atccggtgag gtggcggaaa ggcttcatta tgcctatccg gagtatgata ggaatataac
      661 aaaatatacc agggacatgt ataaaaaata cactggtaaa accggctgcc tggatagcac
      721 atatgccgct gatatagaag agaggcggga acagtgatta cagaaatgaa agcagggcac
      781 ctgaaagata tcgataaacc cagcgaacca tttgaggtga taggtaagat tataccgagg
      841 tatgaaaacg agaattggac ctttacagaa ttactctatg aagcgccata tttaaaaagc
      901 taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata tattgacaat
      961 actgataaga taatatatct ttactaccaa gacgataaat gcgtcggaaa agttaaactg
     1021 cgaaaaaatt ggaaccggta cgcttatata gaagatatcg ccgtatgtaa ggatttcagg
     1081 gggcaaggca taggcagcgc gcttatcaat atatctatag aatgggcaaa gcataaaaac
     1141 ttgcatggac taatgcttga aacccaggac aataacctta tagcttgtaa attctatcat
     1201 aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaaagct
     1261 gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta
     1321 taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg
     1381 ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag
     1441 atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat
     1501 atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg
     1561 acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc
     1621 atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt
     1681 atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct
     1741 ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg
     1801 aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag
     1861 acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag
     1921 aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca
     1981 aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg
     2041 ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt
     2101 ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg
     2161 aattgtttta gtacctagat ttagatgtct aaaaagcttt aactacaagc tttttagaca
     2221 tctaatcttt tctgaagtac atccgcaact gtccatactc tgatgtttta tatcttttct
     2281 aaaagttcgc tagatagagt tctatattag agatgtaaag agttttggtg aagaggtttg
     2341 tgagtatggc ttttataacc agggtgcaat gagaagcact aacagcagct agcttaagaa
     2401 cgcataggat atacttttta tggaagtcac agaggaggaa taaaatttga acaggattaa
     2461 cagagtaacc tctattctta ttcagctgca atcaaaaaaa taattcctgc caaagaaatt
     2521 gcacagcgat ttaatataag cttaaggaca gtttacagag atatccggac acttgaagaa
     2581 gccggaatac caattggatc tgaggccgga aaaggatatt ttcttgtaga gggcttccta
     2641 cttctgccgg taatgtttac agcggcggaa gtgggtgcat tgattacagc agggaaattt
     2701 ttaaattgcc atggagatga atcatttata aaggattttg attcagctat gtataaaatc
     2761 aaatctattt tgaagcatgg tgaaaaaaac tatgcacagg agttggagaa cagtattaac
     2821 gtgtacagca catctggaca gaaaaataca ttggcggata acgttattgc agcaattcag
     2881 actgcaatct gcaataaaag ggtaatatca attcaatatc ctgcatcagg agggcaagaa
     2941 ccggaaagca gaatgataga gcctatatca ctggggtttt atgaacagaa ctggtactta
     3001 atcggttttg caggctaaga aatgaatata ggaattttcg tgtggatcgg attaaaagca
     3061 tttgcataga agaagagctc tgtcaaagcc atgatggctc tttggaacaa atattaaaac
     3121 aaatgctggt tctgttgcaa agtaaaaaaa tatagctaac cactaattta tcatgtcagt
     3181 gttcgcttaa cttgctagca tgatgctaat ttcgtggcat ggcgaa
//
LOCUS       DAKTLH010000362         2127 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.guided.246, whole genome shotgun sequence.
ACCESSION   DAKTLH010000362 DAKTLH010000000
VERSION     DAKTLH010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.guided.246"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            60..2069
                     /gene="mecA"
                     /locus_tag="O0394_002972"
     CDS             60..2069
                     /gene="mecA"
                     /locus_tag="O0394_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCY0500947.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
BASE COUNT          897 a          287 c          360 g          583 t
ORIGIN      
        1 tttgacataa atactacatt tgtaatatac tacaaatgta gtcttatata aggagtatat
       61 tgatgaaaaa gataaaaatt gttccactta ttttaatagt tgtagttgtc gggtttggta
      121 tatattttta tgcttcaaaa gataaagaaa ttaataatac tattgatgca attgaagata
      181 aaaatttcaa acaagtttat aaagatagca gttatatttc taaaagcgat aatggtgaag
      241 tagaaatgac tgaacgtccg ataaaaatat ataatagttt aggcgttaaa gatataaaca
      301 ttcaggatcg taaaataaaa aaagtatcta aaaataaaaa acgagtagat gctcaatata
      361 aaattaaaac aaactacggt aacattgatc gcaacgttca atttaatttt gttaaagaag
      421 atggtatgtg gaagttagat tgggatcata gcgtcattat tccaggaatg cagaaagacc
      481 aaagcataca tattgaaaat ttaaaatcag aacgtggtaa aattttagac cgaaacaatg
      541 tggaattggc caatacagga acagcatatg agataggcat cgttccaaag aatgtatcta
      601 aaaaagatta taaagcaatc gctaaagaac taagtatttc tgaagactat atcaaacaac
      661 aaatggatca aaattgggta caagatgata ccttcgttcc acttaaaacc gttaaaaaaa
      721 tggatgaata tttaagtgat ttcgcaaaaa aatttcatct tacaactaat gaaacagaaa
      781 gtcgtaacta tcctctagga aaagcgactt cacatctatt aggttatgtt ggtcccatta
      841 actctgaaga attaaaacaa aaagaatata aaggctataa agatgatgca gttattggta
      901 aaaagggact cgaaaaactt tacgataaaa agctccaaca tgaagatggc tatcgtgtca
      961 caatcgttga cgataatagc aatacaatcg cacatacatt aatagagaaa aagaaaaaag
     1021 atggcaaaga tattcaacta actattgatg ctaaagttca aaagagtatt tataacaaca
     1081 tgaaaaatga ttatggctca ggtactgcta tccaccctca aacaggtgaa ttattagcac
     1141 ttgtaagcac accttcatat gacgtctatc catttatgta tggcatgagt aacgaagaat
     1201 ataataaatt aaccgaagat aaaaaagaac ctctgctcaa caagttccag attacaactt
     1261 caccaggttc aactcaaaaa atattaacag caatgattgg gttaaataac aaaacattag
     1321 acgataaaac aagttataaa atcgatggta aaggttggca aaaagataaa tcttggggtg
     1381 gttacaacgt tacaagatat gaagtggtaa atggtaatat cgacttaaaa caagcaatag
     1441 aatcatcaga taacattttc tttgctagag tagcactcga attaggcagt aagaaatttg
     1501 aaaaaggcat gaaaaaacta ggtgttggtg aagatatacc aagtgattat ccattttata
     1561 atgctcaaat ttcaaacaaa aatttagata atgaaatatt attagctgat tcaggttacg
     1621 gacaaggtga aatactgatt aacccagtac agatcctttc aatctatagc gcattagaaa
     1681 ataatggcaa tattaacgca cctcacttat taaaagacac gaaaaacaaa gtttggaaga
     1741 aaaatattat ttccaaagaa aatatcaatc tattaactga tggtatgcaa caagtcgtaa
     1801 ataaaacaca taaagaagat atttatagat cttatgcaaa cttaattggc aaatccggta
     1861 ctgcagaact caaaatgaaa caaggagaaa ctggcagaca aattgggtgg tttatatcat
     1921 atgataaaga taatccaaac atgatgatgg ctattaatgt taaagatgta caagataaag
     1981 gaatggctag ctacaatgcc aaaatctcag gtaaagtgta tgatgagcta tatgagaacg
     2041 gtaataaaaa atacgatata gatgaataac aaaacagtga agcaatccgt aacgatggtt
     2101 gcttcactgt tttattatga attatta
//
LOCUS       DAKTLH010000363         1558 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.guided.277, whole genome shotgun sequence.
ACCESSION   DAKTLH010000363 DAKTLH010000000
VERSION     DAKTLH010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.guided.277"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            89..508
                     /locus_tag="O0394_002973"
     CDS             89..508
                     /locus_tag="O0394_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCY0500948.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(764..>1558)
                     /locus_tag="O0394_002974"
     CDS             complement(764..>1558)
                     /locus_tag="O0394_002974"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HCY0500949.1"
                     /translation="ILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVD
                     YGNVYHDHEELIDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSI
                     GVINIDAHFDTRAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKI
                     DYVFADELLSHVSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPH
                     TVLELAKRIIPSDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
BASE COUNT          560 a          275 c          216 g          507 t
ORIGIN      
        1 agatcatcgt ataaaaatta atgacgtcat ttcaaaaatc gatacaaaaa taatttatta
       61 taaaaattct aagaaagaag tgaagcagat gttaaaatct attaatcata tatgcttttc
      121 agtcagaaat ttaaacgatt caatacattt ttatagagat attttacttg ggaaattgct
      181 attgactggt aaaaaaactg cttattttga gcttgcaggc ctatggattg ctttaaatga
      241 agaaaaagat ataccacgta atgaaattca cttttcatat acacatatag ctttcactat
      301 agatgacagc gaatttaaat attggcatca gaggttaaaa gataataacg tgaatatttt
      361 agaaggaaga gttagagata ttagagatag acaatcaatt tactttaccg accctgatgg
      421 tcataagcta gaattacata ctggcacact tgagaacaga ttaaattatt ataaagaggc
      481 taaaccacat atgacatttt acaaataagg tgtcattata aaaaggcctc ttgaactccg
      541 ttaaaatttt aattaattat tatataataa gagaactttt caaacaatac agttgttatt
      601 tttgctattt caacaaacat aaataagcag taagatgact acaacttaag agtcttctta
      661 ctgcaattat ttttcaaata tatcaacgtt aatataactt ctattaagaa atactcacat
      721 tctgccctgc aatgcaaatc tcgtcacata taaatatttt taattatttt aaaaaatgat
      781 gcactaaatt agcaacgagc ttagcagttc tattgtcagc gtcatatgtt ggattcatct
      841 cagcaatact aactgaagac accttatcac ttggaataat acgttttgct aattcaagaa
      901 cagtatgtgg atacaaacct aacactgccg gcgcacttac cccaggcgca aacgcactat
      961 caatgacatc catacaaatc gtaaacataa tgacatcatg ttcatgtaca aaacgttcaa
     1021 tcatatcttt aattgttggt gatacgtgac tcaataattc atctgcaaag acataatcaa
     1081 tctttttctc tttagcataa tcaaataaac tttgcgtatt accaccttga gcaataccaa
     1141 gcactaaata atctgtgttt tcatcttctt ctaaaatttg tctaaagctc gttccagatg
     1201 tagattgttg ttcagcacgt gtatcaaaat gcgcatcaat atttatcaca ccaatagatt
     1261 gtgttggata gactttacgt gttgctaaat attgagcata cgcaatatca tgtccaccac
     1321 ctaataaaaa tgtttgtcta tgattagcaa ttgacttcgc tgcaagcata gcaaattctt
     1381 tttgagtatc aattaattcc tcatgatcat gataaacatt tccgtaatcg actaaagttt
     1441 cacattgatt caaatccggc aaacctgcaa atgcttgttt aatcgcatct ggtccttctt
     1501 ttgcaccaat gcgccccttg tttaaagcaa cacctttgtc aacagcatag cctaatat
//
LOCUS       DAKTLH010000364         1533 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.guided.278, whole genome shotgun sequence.
ACCESSION   DAKTLH010000364 DAKTLH010000000
VERSION     DAKTLH010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.guided.278"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            71..1057
                     /locus_tag="O0394_002975"
     CDS             71..1057
                     /locus_tag="O0394_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCY0500950.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            1029..1289
                     /locus_tag="O0394_002976"
     CDS             1029..1289
                     /locus_tag="O0394_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCY0500951.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(1280..>1533)
                     /locus_tag="O0394_002977"
     CDS             complement(1280..>1533)
                     /locus_tag="O0394_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCY0500952.1"
                     /translation="VFGFMKAILGFTRMSVRGLNKVKRELGFVLMALNIRKVVAQRAE
                     NNQKIYKKDNFYIISIEIVFFHLSKNFMSRTHYDLFFYC"
BASE COUNT          551 a          210 c          255 g          517 t
ORIGIN      
        1 gtagtatatt acaaatgtag tatttatgtc aaaataatgt tataattttt gtgatatgga
       61 ggtgtagaag gtgttatcat cttttttaat gttaagtata atcagttcat tgctcacgat
      121 atgtgtaatt tttttagtga gaatgctcta tataaaatat actcaaaata ttatgtcaca
      181 taagatttgg ttattagtgc tcgtctccac gttaattcca ttaataccat tttacaaaat
      241 atcgaatttt acattttcaa aagatatgat gaatcgaaat gtatctgaca cgacttcttc
      301 ggttagtcat atgttagatg gtcaacaatc atctgttacg aaagacttag caattaatgt
      361 taatcagttt gagacctcaa atataacgta tatgattctt ttgatatggg tatttggtag
      421 tttgttgtgc ttattttata tgattaaggc attccgacaa attgatgtta ttaaaagttc
      481 gtcattggaa tcgtcatatc ttaatgaacg acttaaagta tgtcaaagta agatgcagtt
      541 ctacaaaaag catataacaa ttagttatag ttcaaacatt gataatccga tggtatttgg
      601 tttagtgaaa tcccaaattg tactaccaac tgtcgtagtc gaaaccatga atgacaaaga
      661 aattgaatat attattctac atgaactatc acatgtgaaa agtcatgact taatattcaa
      721 ccagctttat gttgttttta aaatgatatt ctggtttaat cctgcactat atataagtaa
      781 aacaatgatg gacaatgact gtgaaaaagt atgtgataga aacgttttaa aaattttgaa
      841 tcgccatgaa catatacgtt atggtgaatc gatattaaaa tgctctattt taaaatctca
      901 gcacataaat aatgtggcag cacaatattt actaggtttt aattcaaata ttaaagaacg
      961 tgttaagtat attgcacttt atgattcaat gcctaaacct aatcgaaaca agcgtattgt
     1021 tgcgtatatt gtatgtagta tatcgagctt cacatgaaac agctaaagaa gctttgggcg
     1081 ataaagagtt aagagccatt gcacatgagt taactaaaac agttaaggat aacatgagtg
     1141 ttgattggtc taaacgagac agtgctaaag ctaaaatgag agttcaagtt agacgcctat
     1201 taaagaaata tggctatcca ccagatcttc aaaaaatggc tgtggaacaa gttgtagagc
     1261 aagcagaatt aatggcaagt cagcaataaa aaaataaatc ataatgagtc cgggacataa
     1321 agttcttgga taagtgaaaa aagacaattt ctattgaaat aatatagaaa ttgtcttttt
     1381 tataaatttt ttgattattt tcagctcgtt gagctactac ttttcttata ttaagtgcca
     1441 ttaatacaaa accaagttct cttttgactt tattgagtcc tcggacagac atccgagtga
     1501 aacccaaaat agccttcata aatccaaaaa cag
//
LOCUS       DAKTLH010000365          581 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 5902
            SAMN04868257-rid16189873.guided.340, whole genome shotgun sequence.
ACCESSION   DAKTLH010000365 DAKTLH010000000
VERSION     DAKTLH010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868257
            Sequence Read Archive: SRR5415210
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516783.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516783.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:22:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,978
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 15 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868257-rid16189873.guided.340"
                     /isolate="5902"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: MA"
                     /collection_date="2011"
                     /collected_by="FTenover,ITickler"
     gene            166..489
                     /gene="qacC"
                     /locus_tag="O0394_002978"
     CDS             166..489
                     /gene="qacC"
                     /locus_tag="O0394_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacC"
                     /protein_id="HCY0500953.1"
                     /translation="MPYIYLIISISTEVIGSAFLKSSEGFSKFIPSLGTIISFGICFY
                     FLSKTMQHLPLNITYATWAGLGLVLTTVVSIIIFKEQINLITIVSIVLIIVGVVSLNI
                     FGTSH"
BASE COUNT          215 a           92 c           79 g          195 t
ORIGIN      
        1 caagtttagt taagggttct tctcaacatc aataaatttt ctcggcataa atgcatgttt
       61 actgtaaaat tgatactgat acaaataaaa aataaaagga tcgttgcaaa tgaaaaatac
      121 ttagaataaa attaaataaa atacgaaaat taaaaggagt taaaaatgcc ttatatttat
      181 ttaataatat ccataagtac tgaagttatt ggaagtgcat ttcttaaatc ttcagaaggc
      241 ttttcaaaat ttataccatc cttaggaaca ataatttcat ttggaatttg tttctatttt
      301 ttaagtaaaa caatgcaaca cctaccacta aatataactt atgcaacttg ggcgggacta
      361 ggtttagtct taacaaccgt agtctcaata attattttca aagaacaaat aaatctaata
      421 actatagtat ctatagtttt aatcatagtc ggcgtagttt cgttaaacat tttcggaaca
      481 tcgcattaat tgctttattc caattgcttt attgacgttg agcctccgaa cccttaacaa
      541 acccaaaact tgtcgaatgg tcggcttaat agctcacgct a
//
